LOCUS       DAEEGA010000001        84557 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEEGA010000001 DAEEGA010000000
VERSION     DAEEGA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 84557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84557
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.001"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            311..1744
                     /gene="cscA"
                     /locus_tag="KLH46_000001"
     CDS             311..1744
                     /gene="cscA"
                     /locus_tag="KLH46_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBE0376863.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1752..2810)
                     /locus_tag="KLH46_000002"
     CDS             complement(1752..2810)
                     /locus_tag="KLH46_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE0376864.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2978..3196
                     /locus_tag="KLH46_000003"
                     /pseudo
     CDS             2978..3196
                     /locus_tag="KLH46_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3214..4542
                     /gene="dsdA"
                     /locus_tag="KLH46_000004"
     CDS             3214..4542
                     /gene="dsdA"
                     /locus_tag="KLH46_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBE0376865.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4650..6188)
                     /gene="emrY"
                     /locus_tag="KLH46_000005"
     CDS             complement(4650..6188)
                     /gene="emrY"
                     /locus_tag="KLH46_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBE0376866.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6188..7351)
                     /gene="emrK"
                     /locus_tag="KLH46_000006"
     CDS             complement(6188..7351)
                     /gene="emrK"
                     /locus_tag="KLH46_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBE0376867.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7767..8381
                     /gene="evgA"
                     /locus_tag="KLH46_000007"
     CDS             7767..8381
                     /gene="evgA"
                     /locus_tag="KLH46_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBE0376868.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8386..11979
                     /gene="evgS"
                     /locus_tag="KLH46_000008"
     CDS             8386..11979
                     /gene="evgS"
                     /locus_tag="KLH46_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBE0376869.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(12035..13180)
                     /gene="yfdE"
                     /locus_tag="KLH46_000009"
     CDS             complement(12035..13180)
                     /gene="yfdE"
                     /locus_tag="KLH46_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBE0376870.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13254..14198)
                     /gene="yfdV"
                     /locus_tag="KLH46_000010"
     CDS             complement(13254..14198)
                     /gene="yfdV"
                     /locus_tag="KLH46_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBE0376871.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14268..15962)
                     /gene="oxc"
                     /locus_tag="KLH46_000011"
     CDS             complement(14268..15962)
                     /gene="oxc"
                     /locus_tag="KLH46_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBE0376872.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(16016..17266)
                     /gene="frc"
                     /locus_tag="KLH46_000012"
     CDS             complement(16016..17266)
                     /gene="frc"
                     /locus_tag="KLH46_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBE0376873.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17779..18402)
                     /locus_tag="KLH46_000013"
     CDS             complement(17779..18402)
                     /locus_tag="KLH46_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBE0376874.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18699..18974
                     /locus_tag="KLH46_000014"
     CDS             18699..18974
                     /locus_tag="KLH46_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0376875.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(19051..19293)
                     /locus_tag="KLH46_000015"
     CDS             complement(19051..19293)
                     /locus_tag="KLH46_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBE0376876.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19646..20566
                     /gene="lpxP"
                     /locus_tag="KLH46_000016"
     CDS             19646..20566
                     /gene="lpxP"
                     /locus_tag="KLH46_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBE0376877.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20921..20992
                     /gene="ypdK"
                     /locus_tag="KLH46_000017"
     CDS             20921..20992
                     /gene="ypdK"
                     /locus_tag="KLH46_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBE0376878.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(21057..22295)
                     /gene="alaC"
                     /locus_tag="KLH46_000018"
     CDS             complement(21057..22295)
                     /gene="alaC"
                     /locus_tag="KLH46_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBE0376879.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22672..24158
                     /locus_tag="KLH46_000019"
                     /pseudo
     CDS             22672..24158
                     /locus_tag="KLH46_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24173..24907
                     /gene="ypdB"
                     /locus_tag="KLH46_000020"
     CDS             24173..24907
                     /gene="ypdB"
                     /locus_tag="KLH46_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBE0376880.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEVFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24920..25777
                     /locus_tag="KLH46_000021"
     CDS             24920..25777
                     /locus_tag="KLH46_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0376881.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYRARFQG"
     gene            complement(25780..28275)
                     /gene="ptsP"
                     /locus_tag="KLH46_000022"
     CDS             complement(25780..28275)
                     /gene="ptsP"
                     /locus_tag="KLH46_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBE0376882.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28300..29337)
                     /gene="ypdE"
                     /locus_tag="KLH46_000023"
     CDS             complement(28300..29337)
                     /gene="ypdE"
                     /locus_tag="KLH46_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBE0376883.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVIGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29337..30420)
                     /gene="ypdF"
                     /locus_tag="KLH46_000024"
                     /pseudo
     CDS             complement(29337..30420)
                     /gene="ypdF"
                     /locus_tag="KLH46_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30435..31682)
                     /locus_tag="KLH46_000025"
     CDS             complement(30435..31682)
                     /locus_tag="KLH46_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBE0376884.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31704..32030)
                     /locus_tag="KLH46_000026"
     CDS             complement(31704..32030)
                     /locus_tag="KLH46_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBE0376885.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32249..33214)
                     /gene="glk"
                     /locus_tag="KLH46_000027"
     CDS             complement(32249..33214)
                     /gene="glk"
                     /locus_tag="KLH46_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBE0376886.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33418..34674
                     /locus_tag="KLH46_000028"
     CDS             33418..34674
                     /locus_tag="KLH46_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBE0376887.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34789..35115
                     /locus_tag="KLH46_000029"
     CDS             34789..35115
                     /locus_tag="KLH46_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBE0376888.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35256..36494)
                     /locus_tag="KLH46_000030"
     CDS             complement(35256..36494)
                     /locus_tag="KLH46_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBE0376889.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFYIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36830..38032
                     /gene="nupC"
                     /locus_tag="KLH46_000031"
     CDS             36830..38032
                     /gene="nupC"
                     /locus_tag="KLH46_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBE0376890.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38082..40194)
                     /locus_tag="KLH46_000032"
                     /pseudo
     CDS             complement(38082..40194)
                     /locus_tag="KLH46_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40402..40477)
                     /locus_tag="KLH46_000033"
     tRNA            complement(40402..40477)
                     /locus_tag="KLH46_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40442..40444),aa:Ala,seq:ggc)
     gene            complement(40517..40592)
                     /locus_tag="KLH46_000034"
     tRNA            complement(40517..40592)
                     /locus_tag="KLH46_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40557..40559),aa:Ala,seq:ggc)
     gene            40813..41172
                     /locus_tag="KLH46_000035"
     CDS             40813..41172
                     /locus_tag="KLH46_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBE0376891.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41174..41566
                     /locus_tag="KLH46_000036"
     CDS             41174..41566
                     /locus_tag="KLH46_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBE0376892.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41618..43033)
                     /gene="gltX"
                     /locus_tag="KLH46_000037"
     CDS             complement(41618..43033)
                     /gene="gltX"
                     /locus_tag="KLH46_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBE0376893.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43292..43367
                     /locus_tag="KLH46_000038"
     tRNA            43292..43367
                     /locus_tag="KLH46_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43325..43327,aa:Val,seq:tac)
     gene            43412..43487
                     /locus_tag="KLH46_000039"
     tRNA            43412..43487
                     /locus_tag="KLH46_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43445..43447,aa:Val,seq:tac)
     gene            43492..43567
                     /locus_tag="KLH46_000040"
     tRNA            43492..43567
                     /locus_tag="KLH46_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43525..43527,aa:Lys,seq:ttt)
     gene            43598..43723
                     /locus_tag="KLH46_000041"
     ncRNA           43598..43723
                     /ncRNA_class="other"
                     /locus_tag="KLH46_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43999..44340
                     /locus_tag="KLH46_000042"
     CDS             43999..44340
                     /locus_tag="KLH46_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HBE0376894.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44331..45257)
                     /locus_tag="KLH46_000043"
     CDS             complement(44331..45257)
                     /locus_tag="KLH46_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0376895.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45347..46345
                     /locus_tag="KLH46_000044"
     CDS             45347..46345
                     /locus_tag="KLH46_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBE0376896.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46342..46560)
                     /locus_tag="KLH46_000045"
     CDS             complement(46342..46560)
                     /locus_tag="KLH46_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBE0376897.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46562..48577)
                     /gene="ligA"
                     /locus_tag="KLH46_000046"
     CDS             complement(46562..48577)
                     /gene="ligA"
                     /locus_tag="KLH46_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBE0376898.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48648..49634)
                     /gene="zipA"
                     /locus_tag="KLH46_000047"
     CDS             complement(48648..49634)
                     /gene="zipA"
                     /locus_tag="KLH46_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBE0376899.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49864..50625
                     /gene="cysZ"
                     /locus_tag="KLH46_000048"
     CDS             49864..50625
                     /gene="cysZ"
                     /locus_tag="KLH46_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBE0376900.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50810..51781
                     /gene="cysK"
                     /locus_tag="KLH46_000049"
     CDS             50810..51781
                     /gene="cysK"
                     /locus_tag="KLH46_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBE0376901.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            52165..52422
                     /gene="ptsH"
                     /locus_tag="KLH46_000050"
     CDS             52165..52422
                     /gene="ptsH"
                     /locus_tag="KLH46_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBE0376902.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52467..54194
                     /gene="ptsI"
                     /locus_tag="KLH46_000051"
     CDS             52467..54194
                     /gene="ptsI"
                     /locus_tag="KLH46_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBE0376903.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54235..54744
                     /gene="crr"
                     /locus_tag="KLH46_000052"
     CDS             54235..54744
                     /gene="crr"
                     /locus_tag="KLH46_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBE0376904.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54786..55637)
                     /gene="pdxK"
                     /locus_tag="KLH46_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54786..55637)
                     /gene="pdxK"
                     /locus_tag="KLH46_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBE0376905.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55742..56116
                     /locus_tag="KLH46_000054"
     CDS             55742..56116
                     /locus_tag="KLH46_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBE0376906.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            56149..56872
                     /locus_tag="KLH46_000055"
                     /pseudo
     CDS             56149..56872
                     /locus_tag="KLH46_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(57076..57987)
                     /gene="cysM"
                     /locus_tag="KLH46_000056"
     CDS             complement(57076..57987)
                     /gene="cysM"
                     /locus_tag="KLH46_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBE0376907.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(58121..59218)
                     /gene="cysA"
                     /locus_tag="KLH46_000057"
     CDS             complement(58121..59218)
                     /gene="cysA"
                     /locus_tag="KLH46_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBE0376908.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(59208..60083)
                     /gene="cysW"
                     /locus_tag="KLH46_000058"
     CDS             complement(59208..60083)
                     /gene="cysW"
                     /locus_tag="KLH46_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBE0376909.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(60083..60916)
                     /gene="cysT"
                     /locus_tag="KLH46_000059"
     CDS             complement(60083..60916)
                     /gene="cysT"
                     /locus_tag="KLH46_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBE0376910.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60916..61932)
                     /gene="cysP"
                     /locus_tag="KLH46_000060"
     CDS             complement(60916..61932)
                     /gene="cysP"
                     /locus_tag="KLH46_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBE0376911.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62236..63027)
                     /gene="ucpA"
                     /locus_tag="KLH46_000061"
     CDS             complement(62236..63027)
                     /gene="ucpA"
                     /locus_tag="KLH46_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBE0376912.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(63214..64014)
                     /gene="murR"
                     /locus_tag="KLH46_000062"
     CDS             complement(63214..64014)
                     /gene="murR"
                     /locus_tag="KLH46_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBE0376913.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            64178..65074
                     /gene="murQ"
                     /locus_tag="KLH46_000063"
     CDS             64178..65074
                     /gene="murQ"
                     /locus_tag="KLH46_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBE0376914.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            65078..66502
                     /gene="murP"
                     /locus_tag="KLH46_000064"
     CDS             65078..66502
                     /gene="murP"
                     /locus_tag="KLH46_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBE0376915.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66682..67581)
                     /gene="yfeX"
                     /locus_tag="KLH46_000065"
     CDS             complement(66682..67581)
                     /gene="yfeX"
                     /locus_tag="KLH46_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBE0376916.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67677..68252)
                     /locus_tag="KLH46_000066"
     CDS             complement(67677..68252)
                     /locus_tag="KLH46_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBE0376917.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68313..68762)
                     /locus_tag="KLH46_000067"
     CDS             complement(68313..68762)
                     /locus_tag="KLH46_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBE0376918.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68749..69174)
                     /locus_tag="KLH46_000068"
     CDS             complement(68749..69174)
                     /locus_tag="KLH46_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBE0376919.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69388..70257
                     /gene="amiA"
                     /locus_tag="KLH46_000069"
     CDS             69388..70257
                     /gene="amiA"
                     /locus_tag="KLH46_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBE0376920.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70261..71160
                     /gene="hemF"
                     /locus_tag="KLH46_000070"
     CDS             70261..71160
                     /gene="hemF"
                     /locus_tag="KLH46_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBE0376921.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(71166..72218)
                     /gene="eutR"
                     /locus_tag="KLH46_000071"
                     /pseudo
     CDS             complement(71166..72218)
                     /gene="eutR"
                     /locus_tag="KLH46_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72264..72764)
                     /gene="eutK"
                     /locus_tag="KLH46_000072"
     CDS             complement(72264..72764)
                     /gene="eutK"
                     /locus_tag="KLH46_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBE0376922.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72777..73436)
                     /gene="eutL"
                     /locus_tag="KLH46_000073"
     CDS             complement(72777..73436)
                     /gene="eutL"
                     /locus_tag="KLH46_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBE0376923.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74618..75409
                     /locus_tag="KLH46_000074"
     CDS             74618..75409
                     /locus_tag="KLH46_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0376924.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            76129..76524
                     /locus_tag="KLH46_000075"
     CDS             76129..76524
                     /locus_tag="KLH46_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBE0376925.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLHPQLIEQSL"
     gene            76542..76805
                     /locus_tag="KLH46_000076"
     CDS             76542..76805
                     /locus_tag="KLH46_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0376926.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76828..77370
                     /locus_tag="KLH46_000077"
     CDS             76828..77370
                     /locus_tag="KLH46_000077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0376927.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77509..77796
                     /locus_tag="KLH46_000078"
     CDS             77509..77796
                     /locus_tag="KLH46_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0376928.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77835..78041
                     /locus_tag="KLH46_000079"
     CDS             77835..78041
                     /locus_tag="KLH46_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0376929.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78353..78838
                     /locus_tag="KLH46_000080"
     CDS             78353..78838
                     /locus_tag="KLH46_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBE0376930.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78819..79097
                     /locus_tag="KLH46_000081"
     CDS             78819..79097
                     /locus_tag="KLH46_000081"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0376931.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            79090..80550
                     /locus_tag="KLH46_000082"
     CDS             79090..80550
                     /locus_tag="KLH46_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE0376932.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80584..81393
                     /gene="murI"
                     /locus_tag="KLH46_000083"
     CDS             80584..81393
                     /gene="murI"
                     /locus_tag="KLH46_000083"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBE0376933.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81512..82206)
                     /locus_tag="KLH46_000084"
     CDS             complement(join(81512..81960,81960..82206))
                     /locus_tag="KLH46_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0376934.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82725..84098)
                     /gene="eutA"
                     /locus_tag="KLH46_000085"
     CDS             complement(82725..84098)
                     /gene="eutA"
                     /locus_tag="KLH46_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBE0376935.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(84073..84552)
                     /gene="eutP"
                     /locus_tag="KLH46_000086"
     CDS             complement(84073..84552)
                     /gene="eutP"
                     /locus_tag="KLH46_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBE0376936.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
BASE COUNT        21967 a        21406 c        20061 g        21123 t
ORIGIN      
        1 aatccggaaa caagtgagcg tttccggatt cttacacagc cactggatcg gtcaactgat
       61 ccttaactga tcggcattaa tactttggct gacatttttt tcccttaaat tcatctgagt
      121 tacgcatagt gataaacctc tttctcgcaa aatcgtcatg gatttactaa aacatgcata
      181 ttcgatcaca aaacgccata gttaacgtta acatttgcga tattcatcgc atttatgaaa
      241 gtaagggact atatttttat aaaagttaac gttaataatt caccaaattt gcttaaccag
      301 gatgattaaa atgacgcaat ctcgattgca tgcggcgcaa aacgcactag caaaacttca
      361 tgagcaccgg ggtaacactt tctatcccca ttttcacctc gcgcctcctg ccggatggat
      421 gaacgatcca aacggcctga tctggtttaa cgatcgttat cacgcgtttt atcaacatca
      481 cccgatgagc gaacactggg gaccaatgca ctggggacat gccaccagtg acgatatgat
      541 ccactggcag catgagccta ttgcgctagc gccaggagac gataatgaca aagacgggtg
      601 tttttcaggt agtgctgtcg atgacaatgg tgtcctctca cttatctaca ccggacacgt
      661 ctggctcgat ggtgcaggta atgacgatgc aattcgcgaa gtacaatgtc tggctaccag
      721 tcgggatggt attcatttcg agaaacaggg tgtgatcctc actccaccag aaggaatcat
      781 gcacttccgc gatcctaaag tgtggcgtga agccgacaca tggtggatgg tagtcggggc
      841 gaaagatcca ggcaacacgg ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg
      901 ggccttcgat cgcgtactgg cccacgctga tgcgggtgaa agctatatgt gggaatgtcc
      961 ggactttttc agccttggcg atcagcatta tctgatgttt tccccgcagg gaatgaatgc
     1021 cgagggatac agttaccgaa atcgctttca aagtggcgta atacccggaa tgtggtcgcc
     1081 aggacgactt tttgcacaat ccgggcattt tactgaactt gataacgggc atgactttta
     1141 tgcaccacaa agctttttag cgaaggatgg tcggcgtatt gttatcggct ggatggatat
     1201 gtgggaatcg ccaatgccct caaaacgtga aggatgggca ggctgcatga cgctggcgcg
     1261 cgagctatca gagagcaatg gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt
     1321 acgccagcag catcaatctg tctctccccg cagaatcagc aataaatatg ttttgcagga
     1381 aaacgcgcaa gcagttgaga ttcagttgca gtgggcgctg aagaacagtg atgccgaaca
     1441 ttacggatta cagctcggca ctggaatgcg gctgtatatt gataaccaat ctgagcgact
     1501 tattttgtgg cggtattacc cacacgagaa tttagacggc taccgtagta ttcccctccc
     1561 gcagcgtgac acgctcgccc taaggatatt tatcgataca tcatccgtgg aagtatttat
     1621 taacgacggg gaagcggtga tgagtagtcg aatctatccg cagccagaag aacgggaact
     1681 gtcgctttat gcctcccacg gagtggctgt gctgcaacat ggagcactct ggctactggg
     1741 ttaacataat atcaggtgga acaacggatc aacagcgggc aagggatccg cgtcactctt
     1801 cccccttcac gaccttcaat aatatgcaat gcagcttccc gcccgataat gtcatgtgga
     1861 agctgaattg tggtcagcgg cggtaaaaac agatgcccga cgccaaccag attatcaaag
     1921 cccattacag cgacatcctg cgggatacgt acccccttcg ccaaaagaac ctgataagcc
     1981 acaaaggctg cgcgatcgtt accacatatc agaacatcaa aatctggttt gcccgatttg
     2041 aagtgagcat tgagtaaact tgcgagatcg gtgtagtgat catcacctgt tgccatgtga
     2101 aattgtttca cctcagccag atctcgtcca gcatcacgcc aagcctgctc aaatccctgc
     2161 cgacgatacc ctgttgccaa cgcactttcc ggtagccaga agcataatgg ttgacgatag
     2221 cccgccgcga gcaaatgctg tgttgattca tattgtgcag tgtaatcatc agggatataa
     2281 ctgggtaacg ctgggtcatc cgccacacag ttcgccaata caatattttc accatacaga
     2341 gactcaggca gcgtgatatg tcgcagcccc attgtagtat agataatgcc atccggacgg
     2401 tgggcaagca gctgacgtgc cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa
     2461 aaactattcc agccgaactc gctggcggtt tgctcaatgg caagcagaat atcaacagag
     2521 aaaggagtgg tagccgtgtc ctgcgccagc acggcgagag tcgacggctt acgtccttga
     2581 gcgcgcatct tacgggcgga aagatcagga acataattca gggtctggat tgcctgcaat
     2641 acgcggtcac gcgttgcagg acgcacagat tctgcattat gcatcacccg ggagactgtc
     2701 atcatcgaca ctcccgccag gcgtgcgaca tcctttaatg aagccatacc caagccgttt
     2761 gccgtaaaac gggcactgta gcagaaacag acgtcactgg cgagatccaa cgccctatca
     2821 cctgacacag caatacaata aaaaataaca ataattcccg gacaattgtc cccaattccg
     2881 cctctgttct cgccaacgag taaccatata ggcaagtaag taaaacatgc caactggcat
     2941 cgccaacctg gagaagaaaa tataaatatg gatcattgtg ctgccactgt atcccgacat
     3001 cagcccggaa attattgcga ttgctatcgg ttcaggtgca attggctgca cgatcgttac
     3061 ggactcgctt ttctggctgg tgaagcaata ttgcggcgct acgctcaatg aaacatttaa
     3121 atactatacg acagcgacat ttatcgcttc agtcgtcgct ctggcgggca cattcctgct
     3181 gtcatttatc atctaagcgc aaagagacgt actatggaaa acgctaaaat gaactcgctc
     3241 atcgcccagt atccgttggt aaaggatctg gttgctctta aagaaaccac ctggtttaat
     3301 cctagcacga cctcattggc tgaaggttta ccttatgttg gcctgaccga acaggatgtt
     3361 caggacgccc atgcgcgctt atcccgtttt gcaccctatc tggcaaaagc atttgctgaa
     3421 acagctgcca ctggggggat tattgaatca gaactggttg ccattccggc tatgcaaaaa
     3481 cggctggaaa aggaatatca acaaccgatc agcgggcaac tcctactgaa aaaagatagc
     3541 catttgccca tttccggctc tataaaagca cgcggcggga tttatgaagt cctggcacat
     3601 gcagaaaaac tggctctgga agcggggttg ctgacgcttg atgatgacta cagcaaactg
     3661 ctttctccgg agtttaaaca gttctttagc caatacagca ttgctgtggg ctcaaccgga
     3721 aatctggggt tatcaatcgg cattatgagc gcccgcattg gctttaaggt gacagttcat
     3781 atgtctgctg atgcccgggc atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc
     3841 gtggaatatg agcaagatta tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct
     3901 gacccgaact gtttctttat tgatgacgaa aattcccgca cgttgttcct tgggtattcc
     3961 gttgctggcc agcgtcttaa agcgcaattt gcccagcaag gccgtatcgt cgatgctgat
     4021 aaccctctgt ttgtctatct gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc
     4081 gggcttaaac tggcgtttgg cgatcatgtt cactgctttt ttgccgaacc aacgcactcc
     4141 ccttgtatgt tgttaggcgt ccatacagga ttacacgatc agatttctgt tcaggatatt
     4201 ggtatcgaca accttaccgc agcggatggc cttgcagttg gtcgcgcatc atgctttgtc
     4261 gggcgggcaa tggagcgtct gctggatggc ttctataccc ttagcgatca aaccatgtat
     4321 gacatgcttg gctggctggc gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg
     4381 ggtatggccg gacctcagcg cgtgtgtgca tcagtaagtt accaacagat gcacggtttc
     4441 agcgcagaac aattgcgtaa tgccactcat ctggtgtggg cgacgggagg tggaatggtg
     4501 ccggaagaag agatgaatca atatctggca aaaggccgtt aataacgttt caacgcaaca
     4561 tcgcaatcct ttccctgggt gagcgatgct gccgatggcg cagacttaag atccccggtc
     4621 ttacccgcta taaccccctc cttttatcat cacccaacgc ctttcgctgt aaacggcggt
     4681 ttcgcaaacc aaaccaaaac cgtaagtaag ataaaagcaa tagccgccat acggaaaatc
     4741 tcatttgcag aaatagaaag tgactgctgg gttatttcat tattaatttc attaagaact
     4801 cctgaaagcg aaccatagta tttatccata atttgtgacg atgaattaaa cacggggtta
     4861 aattgatcga tggttgctgt caactgacta tggtgtaacg attcgcgtcg tccccacagc
     4921 gtcattgtca acgacgtacc aactgatcct gacaaggtac gaaaaaaatt actcatactc
     4981 gaggcattgg caaatttatt atctggcaag cctgaaaacg aaatcgttgt taagggtaaa
     5041 aagaaacagg caacggcgaa tccctggaaa aactgtggca aaatgatgcc tgtaaaatca
     5101 atcgttggca taaatgtcac agaacgccag taatagcaaa ccgcatacat caaaaaactg
     5161 aatgtcacta acacccgcat gtctatttta ttgccataac gtcctatcaa aggtgaaatc
     5221 aatagtggca tgatgccgat gggcgcataa gcaagcccgg cccatatcgc attatacccc
     5281 atcgttttct ggagtaactg cggcataagg acgatcgctc cagagtaaaa taaatacgcg
     5341 caggtgatac tcacaatacc aatggtgaaa ttacgggact taaacaaact gagatcaaga
     5401 atcgggttct ctgaggtcga ttcccaaatg actaaagaga tcagagaaat aactgatact
     5461 actgttaata ttattattgt actcgagttg aaccaatcca gatcgcgccc tttatcaagc
     5521 ataatttgca agccaccaac accgagcact aacagggtca gtcttggtag attcattttt
     5581 accggtgaag tttcagtttc tcttccttta agtaaggtta agcataatgt caggacgata
     5641 atccccatag ggacattgat taaaaatatc caaccccagc taaagttatc gcaaatatag
     5701 ccgcccaata tcggcccaca tatcggagcg ataatcacgg tcattgacca taatgccaga
     5761 gcaaatgttc ttttttctgg tggataattc cttaataata aactctgtga cagtggaatt
     5821 aacggccccg ccattaatcc ctgaacgact ctaaaaaata tcagcacatc aagattggtc
     5881 gataaactac acattaagga agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt
     5941 aattcgccta ttctttgtgc cagcctgccg gtaacaggga tcgcaatggc atttgctacg
     6001 ccaaacgagg tgataaccca ggtgccttcg tctgttgatg ctcccagaaa gccagatatt
     6061 gtcggtattg cgacgttaga aatagtggaa tccaacattt gcataaacgt cgctaatgac
     6121 aatgcaatag taacgcacca taacgtccca ccggttaatg gtgccggagt tgatttagtg
     6181 attgccatta aagttgtcca ttatgcgaaa taatgttgct aatttctttt tctatcggac
     6241 tggtatcgat aactaaagcc ttactggtat aagcaggcat ggaggtcacg gttgaagcca
     6301 gatcaggcat ctcagcaatg tcttcgtcct tcgtatcaat agttgcagtc atcgataaac
     6361 caatgcgcaa ggggtgttcc atgagttctt ttggatcaag agaaacttca accggtacac
     6421 gctgaacgat tttgatccag ttccctgtcg cattttgtgc aggtaataag gagaacgcat
     6481 tgccggttcc catattgatc cctgtcaccc gaccatgaaa cacaacattt tcaccataaa
     6541 gatcgctgat aatattgact gattgaccaa tccgtacatc cgtgagttgt gtttctttaa
     6601 agttggcatt aacccacatt tgacgtgccg gtacgacagc cattaacgat tgtccggaac
     6661 tcactgtttc gccgacctga acacttcgct gggcaatata gccagtaacc ggactcctta
     6721 tatccgtacg tttaagcacc aaccaggctt ctttggttgc atccgccgct tcaactactt
     6781 gtggctgacg gtttaatggt gtgttcatta ctaaagcttt attcgcttta taagcctgga
     6841 tagcggcatt cagtgccgct ttgctactta ttaacgtatc tttggtatgc tccagcgttt
     6901 cttttgaaat aaccccctgc ttcgctaaag gcacacgacg gttataatct tctaaagatt
     6961 gttgatactg aatacgtgct gaagcgactt cggcactgta ttgtttatcc tgtaagtaca
     7021 gtttattcgt ttgccgaaca atatttgcca gattattttt agctttattg agtgcgatag
     7081 tggcatcagt tttatccagt gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt
     7141 tatgattaac tacagtgaca ctacctgaga cttgtgcaga aattggatct gcattccccg
     7201 tgacataggc gtcatctgta ctaatcatgt cttctaattc cattgaccaa taggcatagg
     7261 cacctgaaaa cgcaataaat aaaactatca ccaataaaga aaagtatttt tttctgttag
     7321 actgtttttt atttgaatta atctgttcca ctattatctc tcatttcgca tagatgaaat
     7381 ttatgaattg aatgaaaaga gtattcattc ttcatatcaa acacagtgca tgtgttgcac
     7441 attatcccag gaacgttgga ggcctaattt aaatgaggtg caaaaaaaac aacagaggac
     7501 taaaccgtgg cttttgcaat acaattctta cgcctgtagg attagtgaga agacttatag
     7561 tgctaacttg aaactataaa tcatcggtat aatccctgat tttattgttg acatttcatt
     7621 tatgccgact atttatatgg tatacttgtc gaattatctt aaaggaaact cagattttct
     7681 tatttttatt gagaaaatga gatgacgcct tatgtctgta ttactacagg gagaagggag
     7741 atgcttcatt gcaaagggaa taatctatga acgcaataat tattgatgac catcctcttg
     7801 ctatcgcagc aattcgtaat ttattgatca aaaacgatat tgaaatctta gcggagttaa
     7861 ctgaaggcgg aagcgccgtt cagcgggtgg aaacacttaa gcctgatata gtcatcattg
     7921 atgtcgatat ccccggagtt aacggtatcc aagtgttaga aacgctgagg aagcgccaat
     7981 atagcggaat tattattatc gtctccgcta aaaatgacca tttttacggg aaacattgtg
     8041 ctgatgctgg cgctaatggt ttcgtgagta aaaaagaagg catgaacaat atcattgcgg
     8101 ctattgaagc tgcaaaaaat ggctactgct atttcccctt ctctctcaac cggtttgttg
     8161 gaagtttaac gtccgaccag caaaaactcg actccttatc gaaacaagaa attagtgtca
     8221 tgcggtatat tcttgatggc aaggataata atgacattgc ggaaaaaatg ttcatcagca
     8281 acaaaactgt cagcacgtat aaaagtcgcc tgatggaaaa attagaatgt aaatcactga
     8341 tggatcttta cacattcgcg caacgtaaca aaatcggcta atcacatgaa gtttttaccc
     8401 tatttttttc ttctctgttg tggtctttgg tcgaccataa gtttcgcaga cgaagattac
     8461 atcgaatatc gtggcatcag tagtaacaac cgtgtcacac ttgatccagt acgtctgagc
     8521 aacaaggaat tacgttggtt agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc
     8581 caaacggcta ccttgttgca taccgattcg cagcaacagg ttcgtggtat taatgctgat
     8641 tatttaaatc ttttaaaaag agcgttaaat atcaaattaa cactccggga atacgcagat
     8701 catcaaaaag caatggacgc gcttgcagaa ggtgaagtcg atatagtgtt atcacattta
     8761 gttacttcgc cgcctcttaa taatgacatt gctgcaacca aaccattgat aattaccttt
     8821 ccggcgctgg taaccaccct tcacgactca atgcgaccgc ttacctcacc aaaaccagta
     8881 aatattgctc gggtagcaaa ttacccccca gacgaggtaa ttcatcaatc atttccaaaa
     8941 gcaacaatta tctcttttac aaatttatat caggcattag catccgtctc agctgggcac
     9001 aatgattact ttattggtag taacatcatt accagcagta tgatttcccg ctatttcact
     9061 cactccttaa atgtagtgaa atattataac tcgccgcgtc aatataattt tttcttgacc
     9121 agaaaagaat ctgtcattct taatgaagta ctcaatagat ttgttgatgc tttaacaaat
     9181 gaagttcgct atgaagtatc acaaaattgg cttgatacag gaaacctggc ctttctgaac
     9241 aaaccattag aactcactga acatgaaaaa cagtggatta agcagcatcc caatttaaag
     9301 gtgctggaaa atccttactc gcccccctat tctatgacgg atgaaaatgg ctcggttcgg
     9361 ggcgttatgg gggacattct taatattatt accttgcaaa caggtttaaa tttttctccg
     9421 atcaccgttt cacacaatat ccatgctgga acacaactta gccccggagg atgggatata
     9481 atacctggcg ctatttatag tgaagatcga gaaaataatg ttttatttgc tgaagccttc
     9541 ataacaacgc cttacgtttt tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa
     9601 aaaggaatga aagttgccat tccatattat tatgagctgc attcgcaatt aaaagagatg
     9661 tatccggagg ttgaatggat acaggtcgat aatgccagcg ctgcatttca caaggttaag
     9721 gaaggtgaac ttgatgctct ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat
     9781 tactatccta atgaacttta tcattttctt attcctggcg ttccgaatgc atcgctttcg
     9841 ttcgcttttc ctcgcggaga accggaactt aaggatatta ttaataaagc actgaatgca
     9901 attcccccaa gcgaagttct gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc
     9961 attgacacat gggacctata tagcgagcaa ttttatattg ttacgacatt atccgtttta
    10021 ttagttggca gtagcctttt atggggattc tacctgttac gctcagttcg tcgtcgtaaa
    10081 gtcattcagg gtgatttaga aaaccaaata tcattccgaa aagcactctc ggattcctta
    10141 ccgaatccaa cttatgttgt aaactggcaa ggtaatgtca ttagtcataa tagtgctttt
    10201 gaacattatt tcactgcgga ttactacaaa aatgcaatgt taccattaga aaacagtgat
    10261 tcacccttta aagatgtttt ttctaatgcg catgaagtca cagcagaaac gaaagaaaat
    10321 cgaacaatat acacacaggt atttgaaatt gataatggca tcgagaaaag atgcattaat
    10381 cactggcata cattatgcaa tcttcctgca agtgacaatg cagtatatat ttgtggttgg
    10441 caagatatta ctgaaacgcg tgatctaatt aatgcactcg aggtagaaaa aaataaagcg
    10501 ataaaggcta ccgtagcaaa aagtcagttt ctggcaacga tgagtcacga aataagaaca
    10561 ccaataagct ctattatggg cttcctggaa cttctgtcgg gttctggtct tagcaaggag
    10621 caacgggtgg aggcgatttc acttgcctac gccaccggac aatcactcct cggcttaatt
    10681 ggtgaaatcc ttgatgtcga caaaattgaa tcgggtaact atcaacttca accacaatgg
    10741 gtcgatatcc ctactttagt ccagaacact tgtcactctt tcggtgcgat tgctgcaagc
    10801 aaatcgatcg cattaagttg cagcagtaca tttcctgatc attacctggt taagatcgac
    10861 cctcaggcgt ttaagcaggt cttatcaaat ttactgagta atgctctcaa atttaccacc
    10921 gagggggcag taaaaattac gacctccctg ggtcacattg atgacaacca cgctgttatc
    10981 aaaatgacga ttatggattc tggaagtgga ttatcgcagg aagaacaaca acaactgttt
    11041 aagcgctaca gccaaacaag tgcaggtcgt cagcaaacag gttctggttt aggcttaatg
    11101 atctgcaaag aattaattaa aaatatgcag ggcgatttat cattagaaag tcatccaggc
    11161 ataggaacaa catttacgat cacaatcccg gtagaaatta gccagcaagt ggcgactgtc
    11221 gaggcaaaag cagaacaacc catcacacta cctgaaaagt tgagcatatt aatcgcggat
    11281 gatcatccga ccaacaggct attactcaaa cgccagctaa atctattagg atatgatgtt
    11341 gatgaagcca ctgatggtgt gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg
    11401 cttattactg acgttaatat gccgaatatg gatggttttg agttgactcg caaactccgt
    11461 gagcaaaatt cttccttacc catctggggg cttacagcca acgcacaggc taacgaacgt
    11521 gaaaaagggt taagttgcgg catgaactta tgtttgttca aaccgttgac cctggatgta
    11581 ctgaaaacac atttaagtca gttacaccag gttgcgcata ttgcacctca gtatcgccac
    11641 cttgatatcg aagccctgaa aaataatacg gcgaatgatc tacaactgat gcaggagatt
    11701 ctcatgactt tccagcatga aacgcataaa gatctacccg ctgcgtttca tgcactagaa
    11761 gctggcgata acagaacttt ccatcagtgt attcatcgca tccacggtgc ggctaacatc
    11821 ctgaatttgc aaaagttgat taatattagc catcagttag aaataacacc tgcttcagat
    11881 gacagtaagc ctgaaattct tcagctgctg aactctgtta aagaacacat tgcagagctg
    11941 gaccaggaga ttgctgtttt ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc
    12001 taatgtggga gctatttacc agcacatctt acatttatga tgagaactcc tggcgaattt
    12061 gttccccatg ctggtcgagc gttgccgctc ccggcataac atgcgggtcc gcgcagccgc
    12121 tgatttttat cggattaccc ggcatcatta ttcctccggc ttcaatcaac atatttctcg
    12181 cctgagtttg tggcaaatta atggcctcag ccacacttaa taacggcgcg acgggcacac
    12241 caacttcatg tattttggct aaccaaactt cagctgcctg cgtttttaac gttcgctcaa
    12301 tatattgttt aagaatagcc tggttttgta cgcgtaaaat attgctgcta aatcggggat
    12361 cattaaccag ttccgtaagc tccagtgcct ggcataacgc agaaaaaagc ttgtcattac
    12421 cacaacaaat cgtaatcggc ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg
    12481 gatggcgatt tcccaggcgt tgtggtgact tccctgtcgc aatatatgcc atcagaccat
    12541 gctccagaaa actcagcgtg gcatcaaaca tcgctatatc gacatgcgcc cctctctggc
    12601 tcttttcgcg gccataaagt gcactcacta ttccgctgaa taaatagaca ccgccgcata
    12661 gatccgcaag agaggtacca acacgcactg gcggagcatc agggtaccct gtttccatca
    12721 ttatcccact cattgcctga atgatggtat cgtaggcagg agcatctttt agcggaccgg
    12781 tatgtccgaa acctgacgat gaagcatata tgaggcgcgg gttgatttct tgtaaccttt
    12841 cccatgaaaa ccccagtttt tccattgtac ctaggcgaaa attctcagct aatacatcag
    12901 cttgtttgag catatttata aatatacttt tatcgtgatc attctttaaa tcaagaacca
    12961 cactctcttt gccatgatta ataaaactgt aatagagtga ctgtccatcc acatagggac
    13021 caaatgtgcg ggtatcatca ccatgacctg gtggctcaac tttaattacc cttgcgccca
    13081 tattataaag aagttgagtt ccgaaaggtc cattaaggac atgtgtcata tcgataacta
    13141 ataagccttc aaacggccct ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa
    13201 gccgaaggtg aaaagacact tcggcattta tgtaaaatca ataacatgat taattatgaa
    13261 accagtcgac tgacataaat ccacaaggga gccgtgacga caaagcccag tacgctcaca
    13321 gccaatgacg ctgtaccagt acgggtgtag acattaaacc ggctggcaat aatgattccg
    13381 gagaatgccg gcggtaatgc gcctgccagt accatcatct gcaaatgttc gctgttcaaa
    13441 tgacatgcca taccaacgag aagcagcgcc agtggcatca gaatcaactt caggaaggtg
    13501 ttataagcaa tttcggcact gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg
    13561 aataccgcta cccctgagtt agccttcgca atcagattaa aggttgggtc ccatgctgcc
    13621 ggaattttta ccccaaccag caccaggatc gttgccagaa caggtgccca tactactggc
    13681 tcctttgccg cagaaattaa tgcgctcaga ttactattct tcttaccatc cgctcctgag
    13741 gaaggattca gcaaatacag accaatagga atagtaattg cgttaacaat aatagaaata
    13801 attgctacca ctaaacctgt tgatacggaa tcaccataaa taggatcgag aactgcaaac
    13861 cccaggaatc caatggtagg tgaacccgca attaatgcac atacagctgc ttctgcatgg
    13921 gtacgtttaa aaaatttgta gcaaccaaac caggagaaaa agaaacatcc gacaataacc
    13981 acaagtgata caagggtcag acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta
    14041 atagaaacaa atagagccgc aggaagagcg tagtttaata ccagtttatt aaatgcgcgg
    14101 gcttgatctt ctgaaaaagt ttctcgtctg ccgctaaaat aacccaacag catgattacg
    14161 ataataggca ataaatcgcc aataaaaaat gttagcataa tagtatttcc gataagggtc
    14221 ccctcaccat cgcataatga gttggcaaga ggagacgatg tcagtattta attaccagcg
    14281 acttgttttg ggttaagttt ggtaatatgg ccactttcag ttcctgctgc agggtcgata
    14341 accacattaa taatggtcgg tttgcgcgac tgaataccgg tggttaaagc atgacgaagt
    14401 tcatctgtcg tggtgacgtt atagccaacg ccacgaaacg catccattaa tttgtcatac
    14461 cttgcatggt gcaacagatc cgttggtgat ggtgcaccag cgccactgag atcaacaccg
    14521 tctcctctgt agatgccgcc attattaaaa ataacgatcg tcaccggcag gttatatcga
    14581 caaatcgttt caatttccat cccactgaaa ccaaaagcac tatcaccttc aatggcgaca
    14641 accggagaac cagaagtcac gctagcaccg atggcatagc ccataccgat gcccatgaca
    14701 ccccaggtac cacaatccag acgacgacgt ggtttataca tatcaataat atttcgtgca
    14761 ttatccaggg tatttgcacc ttcattaact aaataaatat cctggttctc gcgcaatacg
    14821 tcgcgcacag cacttaatgc attaaagtaa tttaatggtt gagtatctgt acttaatttt
    14881 tcatgcattt tttgtgcatt ttgctgcttg tggatattta aaatatcgcg ccataccagt
    14941 ggagtcgtaa atgtgttttg tttcagttct gccagcatac cttgcatact ggatgcaata
    15001 tcaccaacga ctggcacagc aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc
    15061 agttgaataa actgtgtatc tgccgcccat ccttttttac cgtgtgccaa taaccaattc
    15121 agtcgtgcac caacaagcat gacaacgtca gcatttgcca gggcaaacga acgcgcagct
    15181 gccgcagaaa gtggatgcgt atcttcaagg atccctttcg ccatagacat tggcaggaat
    15241 ggaatctgag cactttcaat aaattcacga agctgttcat cagcttgtga atacgccgcg
    15301 cctttgccaa ggataattaa tggccgttca gcttttgcta aaagcgaaat tgcgctagtg
    15361 actgacttcg ggcatggcaa taatgctggc gacggatttt caactttaac aatcgtggtt
    15421 aacgcttcgt ctttttccat cgtcgcggcc aggacatttg ctggcaaatc aagataaact
    15481 ccgccagggc gacccgatac agagacccgg atagcgcgtg ccaatgcaat gccaagatcc
    15541 tgcggctgat taacgcgaaa tgctgctttg gcatacggtt ttgccgcatt catttggtcc
    15601 agctcttcat aatcaccttg ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc
    15661 atgataatcg gaaaaccatt taccgttgcg ttggccaatg cggtcaaacc attgaggaat
    15721 cctggcgcag aaactgtcag gcagatcccc ggtttttggg taagaaaacc gcttgccgca
    15781 gcggcatagc ctgccgactg ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg
    15841 gcatggcgtg ccatatccgt cacaggaata cctacaacac cataaatagt gtcaatatta
    15901 ttctgtttta atgcttcaac gatgatatgc ataccatctg tcatttgaag ttgatctgac
    15961 atgattaata acctcgaaat ttttataaat tgggagccag aagcccccgt taatatcaga
    16021 tggcgtggtt ttgcttcatt gcagcaattt catcgtcgct ataacccagc tcctgcaata
    16081 cagcagcggt atgttcacct aatagcggcg cagctttaat atccggcgta aaggcagaga
    16141 atttcattgg acaaccaacg gtcagatatt ttccacgcaa cggttgttcc acttcaacaa
    16201 cactgccact ttggcgcaaa gagggatcaa gtgaaatttc tttcatactt aaaaccggtg
    16261 cacaaggaat atcaaactga gtcaaatagg ccaccgcttc atgtttatca atagtgacag
    16321 tgtatttttc gatttcagca aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac
    16381 tgtatgccgg atcggtaatc cattctggtt tgccgatggc tttacaggtg ttttcccagt
    16441 tttgctcctg aatagtgaaa taaatatagg cgttaggatc ggtttcccag cctttacatt
    16501 tcaggatcca gccaggctga ccgccaccac ctgcattacc accgcgggga actgcatcac
    16561 caaatgtacc attcggatac tgcgggtatt cttccagata acccaattta tcgagacgct
    16621 gctggtcacg taatttcacg cggcaaaggt tcaatacggc atcctgcatt gacatggtga
    16681 ctcgttgccc acgccccgtt ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca
    16741 aatgcattcc ggtgttgctg tcacccaacg ctgcagcgct taccagcggc ggaccatccc
    16801 aaaaacccgt agtggatgcc gcgccacccg ctgcctgagc aacgttttca taggctttta
    16861 cattcacata aggcgaacac tcatcaaacc ctttgatcga accaaaaatc agacgtggat
    16921 tgatttcttg aatatgctcc caggtgaagc ccatgtgatc aatggcccct ggatgaaagt
    16981 tctcgactaa gatatcagct tcgcggatca gcttttccat tacctctttg ccttccgctg
    17041 ttttggtatt taactcaata gaacgtttgt tactgttaag catggtgaag taaagcgcat
    17101 cgatatcagg aatatctcgc agttggtgac gcgttacgtc accaacgccg ggacgttcaa
    17161 ttttaataac gtcagcgcca aaccaggcca gcatttgagt acaagatggg ccagattgca
    17221 caccggtgaa atcgagaact ttaattcctt gaagtggagt tgacatagca atacctatta
    17281 gttaattaaa tatgtgttaa tttagcgttt atttaaggca taacgccctc tataaataat
    17341 ctgcaaaata tcttcgtagc ggcactcgat atttttaaat tcctgtatca agtactgaaa
    17401 attattttat cgccacttcc aattcctttc tgcatggtaa gtagtgagga cgaaaagaag
    17461 aatatgataa atattgattc gtggtaatca gagagagtcg cgacatttgt gatgttattc
    17521 ttacaactgt aagaagcgcc ggtgatttac aagtaagagt atgattacgc tttttaaagc
    17581 gtaccataaa gcataacata ataaatgatt tgtgatgaca cgggattatt gcggtttact
    17641 ttgaggaaaa taaaatgcgt gtttatagaa ccgcgagcgc caaataattt tatgaccaca
    17701 acctgtcgta ttgtggtcat aaaaaattgt cggctggagg gagttaaatc ctttacctca
    17761 atatactgcc tgtcagcatt agttcacaaa cagtgcttcg ctatcgacga tgattccatc
    17821 ttccgcacct ttaagcgcca ggtttgcctc gtagtattgc tttttatcaa gcagtttctg
    17881 cgcatcagca agtgcattac gagtctgttt aagcggcatc agatattggt tttccattac
    17941 gccaacaccg gccaggcgaa gctcttccat tgcgcccttt ttatcccctt tcgccatttt
    18001 ttcattggca atttttatcg cggcttcttt ttcgggtgtt gctacataac tctcagaaat
    18061 tccgacagat gcattaatga caatatattg gtcatcattg agattggtct ttttacccgg
    18121 cttagcgaac tttgcccatt cggtactgtc atctgacagc aaagcggaag cttcattggt
    18181 cagttctttt gccttttccg gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc
    18241 acgcatcgca tataaacctt gttcagagat acgctctgct gctgccgttt tctgggtttg
    18301 ctgagtttgc tgttgtgccg ctaccggtgc attatcagca gcccatacag ttgaagatgc
    18361 cagaattgct gtgaccatcg tagccataat taaacgtttc atacttacct cattctttaa
    18421 cagttttaga aaacgccgcc atcatattgg gtgagcatga aatgagaaat cagagagcaa
    18481 cggtcatttt gaaagggtta tcttacaatt gtagttatta tcgcaaagcg gcatttagtc
    18541 ttacagttgt aggaatatgc ctcctgtcgc agagaactat tcttatatac cacaaagaga
    18601 tgtcatttat tatattgatg taaatattga ttaattatat gtaaatacag ccataacatc
    18661 gtatggttct cagtgaacca ttttcaggat aactttctat gaaagtaaac ttaatacttt
    18721 tcagcttatt tttattggtc tctattatgg catgcaatgt ttttgcattt tccatttctg
    18781 gtggtgtaag tgagaggagc tataaagaga ctgaaaaaac atcagcgatg acgaccacac
    18841 actctacaaa acttcagcca tcacaggcga ttttgtttaa gatgagagaa gatgtgccac
    18901 cattaaacct cacagaagaa atcacgccta cttatccgac aaaggcgaat tatcttattc
    18961 atcctgtgcg atagcgtctg tcacatggca ggtgaacaat catgtctgat gctgatgtga
    19021 acacctgccc aaatgagcca tcctgaccca ctatcgcctg ccatacccca ccataaagcg
    19081 aatcatcaag ccacaagccg gggcaagaaa cataaatatc cccgtcacca gttcattacc
    19141 gccagttaac cacatggtga agtaataaat gagtgcagcg aggatcacca tgccgaagaa
    19201 agaagagacc gccgatacca cgttcagtac ctgaccgatg tagccgctta cgcagacaat
    19261 acacagggcg agaaacatcc ataaataaat catagctcaa cctcctgccg cgctaaaaaa
    19321 cctgtaactt cagtcacttt aatagtaaga caatccttaa cctctggcaa tttttgctaa
    19381 gcatcaatga aaacagcacg ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt
    19441 gcacgattaa taatcagtag ctgaaagcag tcagccagtg ataaagtaga gccaccgcat
    19501 tcatgctcct cgtggttatg tcctgactag tctttacact ctttacagga accattgtcg
    19561 tacatgatgg cccaaccaat tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa
    19621 caattcgtat ctacagaagg taactatgtt tccacaatgc aaattttccc gcgagtttct
    19681 acatcctcgc tactggctca catggtttgg gcttggtgta ctctggcttt gggtacagct
    19741 tccttatcct gttctctgct ttctcggcac gcgtattggc gcaatggcgc gaccattcct
    19801 gaaacgtcgt gaatctatcg cccgtaaaaa cctggaactt tgtttcccgc agcattctgc
    19861 ggaagaacgc gagaagatga ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga
    19921 aaccggcatg gcatggttct ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg
    19981 gttggataac cttaaacgcg cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca
    20041 ttttatgtcg ctggaactgg gcggccgcgt gatgggactg tgccaaccaa tgatggctac
    20101 ctatcgtcca cataataatc agctgatgga atgggtgcag acccgtgggc gcatgcgctc
    20161 taacaaagcg atgatcggca gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg
    20221 tgaagcggta tggtttgctc ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc
    20281 gttctttgcg gtggaaaatg tcgccacaac caatggcacc tatgttctct cccgtctctc
    20341 tggcgcagcc atgttgaccg taacgatggt aagaaaagcg gattacagcg gatatcgttt
    20401 gttcatcacc ccagagatgg aaggctaccc gacagatgaa aatcaagccg ctgcttatat
    20461 gaacaagatt atcgagaaag agatcatgcg cgcaccggag cagtacctct ggatccaccg
    20521 tcgctttaaa acgcgcccgg tgggagaatc gtcgttgtac atttaacaaa ctgctcttac
    20581 tctttccctc ctcaaatcgg ccatgataac ctcatgattt ttatagcttt tttcttaagt
    20641 tttttccttc gctgaatatc taattattac cgtcagaaat taaactgtcg ccgatatgag
    20701 acacttgtaa gttactaaaa gtaaataaga aaatgcctct tgtctcgttt cagttttagg
    20761 cgtacattag cgccgtctcg agcagtttag ctcagaattc tggggtgatg cccacggtaa
    20821 aatacagggc ttttaaggtc gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa
    20881 gtcattcagg cgtaacagta attacgcgga gagatgtaaa gtgaaatatt tctttatggg
    20941 catttctttt atggtcatcg tttgggccgg tacttttgcc ctgatgatct aaaagcaaac
    21001 tgtgcaatga aaaacaggag ccatcaggct cctgtttgca tttctgacga cggtgtttat
    21061 tccgcgtttt cgtgaatatg tttgctgctg acgggtagta aaccgtcggc acggaacatc
    21121 gctttaatcc ctctgatggc ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga
    21181 acatgcgtat caccatagtc gccaaagcca atccccggcg agacacaaac cttcgcttcg
    21241 ttaaggagct tcttggcaaa ttccagcgat cccatggccg catatggttc cgggattttc
    21301 gcccagacat acatcgaagc cttcggcatt tcgaccatcc agcccgcttc atgcagccct
    21361 ttaaccaata catcacgacg gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga
    21421 tcgccctcga gtgccgcaat cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga
    21481 tagcttttga ttcgcgccag ggcactgacc agcgttttgt tgccgaccat aaagccgata
    21541 cgccagcccg ccatgttgta gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt
    21601 gcacccggca cctgcataat tgacggcgct ttccagccat cgtagacgat atcggcatag
    21661 gccaggtcat ggaccaccag cacatcgtag catttcgcca gcgccacaac cttttcgaag
    21721 aactccagtt ccacgcattg cgcggttggg ttcgacggga agccgaggat catcatcttc
    21781 ggtttcggat aactttcacg aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc
    21841 accagcggca ctgagcgtac ctgcgccccg gcaatcaccg cgccataaat atggatcggg
    21901 taacttggat tcggcaccag caccgtgtca ccatgatcca gcgtcgccag catcagatgc
    21961 gccaggccct ctttcgaacc aatagtgacg atggcttctg attccgggtc gatttcaacg
    22021 tcgtagcgat cctgatacca gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa
    22081 gtggagtaac catgcgtgtc cgggcgctgg gccacagtac ataatttttc gacgatatgc
    22141 ggcggagtcg caccgtccgg gttacccatg ctgaaatcga taatatcttc gccgcgccga
    22201 cgcgcagcca ttttcagttc agcggtgatg ttaaaaacgt agggcgggag acgatcaatg
    22261 cgcgtaaagc gacgttcagg gcgagtgtca gccataattt tctcagatta acgttagcgc
    22321 ccggaccgtc cgagcgacgc tgccacaatg gtggctgttt tgaaaatagc ctgattaatt
    22381 tctgtctgtc cagcgtttta gaacattttt atcaatggat ttaaatgagg aactccactt
    22441 caaaaacagg aaatttttct acgctaacaa gaaaatcacc accaatttta ttaacattat
    22501 aatttcaata aatttaccag ctagaagtgc attatcaaga gatacattgc taatacacca
    22561 tttatctttc aatccccttt taaatttatg gtttttcctc aaaaacggcc tgctggtcat
    22621 cagccttcag gttacctatc atagaggttt aatccttatt cagagtcacc cgtgcacgaa
    22681 atattcaaca tgctgctggc ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc
    22741 tttctcatcc gtatccgcct gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa
    22801 gaattgctcg ccgttaccgc cattttctcg ctgttcgccc tgttcagcac ctggtccggc
    22861 gttcccgtag aaggctcgct ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt
    22921 ctgtttggcc cgtgggtagg catcattacc ggcgtgattg cgggtattca ccggtattta
    22981 attgatatcg gcggcgtgac ggcgatcccc tgctttatca ccagcattct ggcgggttgt
    23041 atatcaggct ggataaacct gaaaatcccc aaagcacagc gctggcgctg gatatcgcca
    23101 acaaaacgct accgctgttt cgccatgtca atagcgagtc attacgcaag gtctgcgaaa
    23161 ttatccgcga tgacattcac gccgatgcgg tggcgattac taataccgat catgtgctgg
    23221 cctatgttgg cgtgggtgaa cataactatc agaatggcga tgacttcatt agcccgacta
    23281 cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca
    23341 caccagagat tcattccatg ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa
    23401 cgctgaaaat ttactactgc cacgcgcatc agatcacctc gtcattacag gaaatggcgg
    23461 tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc acgcgccgag cagctacgtg
    23521 aaatggcaaa taaggcagag cttcgcgccc tgcaaagcaa aattaatccc cattttctgt
    23581 ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt
    23641 tgatctttaa tctgtcgcgt tatctgcgct ataacattga attaaaagac gatgagcaaa
    23701 tcgatatcaa aaaagagctg tatcaaatta aagactatat tgccattgag caggcccgct
    23761 ttggcgacaa gctgacggtt atctatgata ttgatgaaga ggtgaattgc tgcattccca
    23821 gcctgctgat ccagccgttg gtggagaacg ccattgtcca cggtattcag ccttgcaaag
    23881 gtaaaggcgt tgtcaccatc agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc
    23941 gagataccgg gcacggcatc gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc
    24001 cgggcaataa aattggcctg ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg
    24061 ggctgcatat ccgccgcctg gagccgggga cggaaattgc gttttacatt cctaaccaac
    24121 gcaccccagt cgcctcacag gctacgttat tgctttgagc cggagtgata ttgtgaaagt
    24181 catcattgtt gaagacgaat tcctggcaca acaggaactg agctggctaa ttaaagagca
    24241 cagccagatg gagattgtcg gcacctttga cgacggtctg gacgtgttga agtttttgca
    24301 gcataaccgc gtcgacgcca tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt
    24361 gctggcgcaa aacatcagcc agttcgccca taaaccgttt attgtgttca tcaccgcgtg
    24421 gaaagaacat gcggtagaag cgtttgaact ggaggtgttt gactacattc tcaaaccgta
    24481 tcaggagtca cgtattaccg ggatgctgca aaaactggaa gcggcctggc aacaacagca
    24541 gaccagcagt acgcctgccg cgacggtaac gcgtgagaat gacaccatta atctggtgaa
    24601 agatgagcga ataatcgtca cgccaattaa cgatatctat tacgccgaag cgcacgagaa
    24661 aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt
    24721 ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat cgctcatttt gtgtcaatct
    24781 gaacaaaata cgcgaaatcg aaccgtggtt taataacacc tacattctgc gactgaaaga
    24841 tctggatttt gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc agttaatgca
    24901 tctttaatcg ggaactttca tgaaagcacc aggcttgccc gccgatcaac aatttttcgc
    24961 cgatctgttc agcggcctag tgcttaaccc gcaactactg gggcgcgtct ggtttgccag
    25021 ccagcctgcc tcgttaccgg tgggcagttt atgtattgat tttccccgtc tggatatcgt
    25081 gctgcgcggc gaatacggca acctgctgga agcaaagcag cagcgaatgg tggaaggaga
    25141 aatgctgttt attccggcac gcgcggctaa tttaccgatc aacaacaaac cggtgatgct
    25201 gttaagcctg gtgttcgcac cgacctggct tgggttatcg ttttacgata gccgcaccac
    25261 gtcgttgttg catcctgctc gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga
    25321 agcgatgctt accgccctca cccatcttag ccgttcgccg ctggagcaga atatcattca
    25381 gccactggtg ttaagtttgc tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa
    25441 ttcacaaccg cgcggcgatt ttctctatca cagcatttgt aactgggttc aggataatta
    25501 tgcccagccg ctcacccgcg agagcgtggc gcagtttttt aatatcacgc ccaatcatct
    25561 gtcaaaactg tttgctcagc atggaacgat gcgttttatc gagtatgtgc gttgggtgcg
    25621 aatggcgaag gcgaggatga ttttgcagaa atatcatctg tcaattcatg aagtggcaca
    25681 gcgttgcggt tttccggata gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct
    25741 gacgccggga gagtacagag cccgttttca gggctaacgt cagaaggtta attctgtttc
    25801 cagcagcgtc aggatacttt gtgcatcttg cgcggcaaac aacgactggc ggaagttttt
    25861 attcaccagt ttacgcgcca gctgcgagaa gactttcaca tgattcatcc cttcgttagc
    25921 acccagcgtc agcatgatca ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg
    25981 tttcgccagc cgggcaatgc tgatactgga atgacggatc cactgagatt tggtgtgcgg
    26041 gatcgctacg ccaaaaccaa cgccggtggt aacaatctct tcccgctgcc agacatcttc
    26101 ttccagctca aacggatgtt cagtgcgccc gttaacgccg aggttgccgc acaggaactg
    26161 gatcgcctgc tctttattgc taaaatcctg atcaacaaag atattctcca gcgccagcag
    26221 tgggcgaacg tcttcttccg gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact
    26281 gcggcattca catgcctgac gcgccagttc ccgacacgcc tcgctatcca gttgacgaag
    26341 ctggcttttc accgccggaa tacgcgggct actcatactc agctcatcca ggcccagccc
    26401 aagcagtagc ggcagataac ggctttcacc gcccagttca ccgcaaatgc ctacccattt
    26461 gccccgctga tgcgcagtgg taactatttg ctgcaacatg cgcaggaacg atggcgtaat
    26521 cgggttatat agcggcgata cgcgcgggtt attacgatcg accgcataca gatactgggt
    26581 catatcgttg gagccgatac tgaagaaatc gacctcatcg cagaagtggt cgatgatgta
    26641 gcacaccgac ggcacttcca ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc
    26701 atcgcgctta agctcaacga tcgctttttg gatctcgcct ttcacccata agatctgatc
    26761 gaggctgtga accatcggga tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat
    26821 ggcccgcagt tgagtgcgga acaggccagc aaattccggg taaatacgta ccgcgcgata
    26881 gccgaggaac gggttctctt cctggggaat attcagataa ggaatgcttt tatcgccgcc
    26941 gatgtccatc gtgcggaaga taatcggctt gtcgcccgcc gccagtagca cctgctggta
    27001 ggcttcaaat tgctcctgct cgtccggcgc gctgtcgcga tccatataga gcatttcggt
    27061 acggaacagc ccgacacctt ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt
    27121 gccgatattc gccgcaatat caatacgctt gttatcacgg gaataggcca gctgcgcggc
    27181 agcctgcgcc tgttgttttt ggcgtttatc cgccagcgtc tgcgcgacct gataataacc
    27241 gctcaccgcg tcattcgggt taatcgccag cacgccgcac tgggcgtcaa gcactgcagg
    27301 ttgcccggca taacgggcaa tcgcatccag cggcaagcca ctcagtaccg ggatcgccga
    27361 ggcacgggcc agaatcagtg tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc
    27421 aagatttttc aaatcaaggc tcaaaaactg gcttggggtt aaatcttcag ccaccagaat
    27481 ggtcggtttt tccagcacca gattgttgcg cggcttcagt tccggccagg tgatatgcag
    27541 caactgttcg ctgatatcgc gaatgtcgct gacccgctcg cgcagataat cgctggcaga
    27601 agcagatagt ttggcgcaaa cctgctccat attgctgatg atcgccgccc ccagtccctg
    27661 atgctgttct gtcatcaggc gacggatatt gcctgcaaat tcatcatcct gaatcagcga
    27721 caaatgagcg ctgaggatag ttttgctttc gccgtcacgc tcacgcaatt gctgattcag
    27781 ttgctcggca agcgttgcca ggctgtgctc cagccgggtg gaatcttgcg cactggcggg
    27841 gattgcccga taactgtcga ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac
    27901 gccgcttgcc agcacattgc cgtacagtaa atccgggtta aggcggctta atgaacgcgg
    27961 cagcggatgc gccgtcagtt ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg
    28021 caccaggatg tactcttcca gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt
    28081 caggctgcaa ctgtcattaa ataaggtgcc agttccaatc aacgccagcg agcttttagc
    28141 atcagccttt gcgttctggc gatggttgat aaaggtgatt tcgctttgcc actggctgca
    28201 ctgttcttta agttcccacg ccggacgagc atgtagaccg ttaggcaaag gacagagaaa
    28261 ttgaatcgtt aacataaccg ctccgggtta atcaggagat catctgaaat ccgtcagttg
    28321 aacaaccgtc tcacgcgtaa gacgttgaat aagggcagat aaaagttgct ccatctgcaa
    28381 aatgtcgcgg caatcggcaa tcgatgccgc gcaatgtccg tggcgggtgg caggccctat
    28441 taccaatgtg ggtacgccag taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt
    28501 actgaacata tccgcctgca acggaacgcc aatttctgcc gccacggttt caatccacgc
    28561 ggtaagtttt ggcggcgcaa tcagcgattt atcgcttaac accagcatcg gcccgttacc
    28621 aatctgacga tggttagccg cgccataatc aaagtttttc gcccagcagg cggtatcaag
    28681 cacaatggcg acgtccggcg acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac
    28741 ctcttcgctg gaacttgcca ccagccacac ttccgcaggc agttcggcgt cgtgcagctc
    28801 acgtagtaac gtcaccagca gatagcaact gaggcgatca tcaaaggctt tgcccatcac
    28861 ccgctggtga gggaggacct gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat
    28921 tcccgcctgc atcacttcgt cataggtgcg cgcgccaatg tccacgcgca tggcgctaac
    28981 gtcattcccc tgccggtcgc cgtcaagcag gcctggaatt ttgcactctt cacgggtggt
    29041 gatgcgcacc ggctgcagct ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc
    29101 aatagcgcct tcgcgggaga tgctgcgcac cataaatccc acttcgtcca tatgggcgca
    29161 gatcatcacc ttcggacctg tcgattcatt gaggcggatc agcaccgatc ccagaccatc
    29221 aaatcgcact tctttttgca ggcgagccgc ttcttccagc aggatctgcc gcacttcctg
    29281 ttccgaggag gcgatcgcat ctgcctcgct caacgctttt aatagcgata aatccattat
    29341 gcctctcccg tgagcaacac tgttttcggc atggcgtaga gcacttctgc gccttgcggg
    29401 gtaaccagca caacatcttc gatgcgcacg cccccttgcc ctagcaaata aatccccggc
    29461 tccacggtca gcaacatgcc tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga
    29521 tcttcatgga cttcaatgcc gatagcgtga ccagtgttat gaccgaaata gtcgccaaat
    29581 ccagcctcgg taatgacccg gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg
    29641 cgaattgcag agattgccgc gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc
    29701 gggtgagatt cggcgctcac cccttcgcca ttcaccagca aggtgcgcgt catatcagca
    29761 gtagccctga tacagcgcac caaaatcgag agtgacaaac tcgcccgctg caacaatctt
    29821 gtcgctggct ttgccgtgcg gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa
    29881 agaggctttt tctgcgccct gctggcgcat aaaccactcc agttcagcgg ctatctcgcg
    29941 ctcgctcatc cccgcctgaa taaagcggcg aatatgctcc gctccgcgat cggcaatccc
    30001 acaggcgagg cggattatct ccacctcctc tggcgttttg atttgccgca gcacatccgg
    30061 cgtggcgcta accagtttcg cattgagttc agacttccag cggtgcgcgg tttcccaact
    30121 cacctgctgg ccttcaaaac cgagcgtttg caactgctca tcggcaatga tttgattgac
    30181 gatagtggta agcgtgttcg tcgcgtcaag caaatgcagc tggtagcctt gcgcacgggc
    30241 ttccacctca acgaaatagc gcgaatctac cagaatgtgc gcactttcac ggctaataac
    30301 cacatagcct gatccggtgg agatccccag atgcggctgt ttgttctgcc gtgaggagag
    30361 aagcaccgca tccagttgtt gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat
    30421 ctggattttc cttcttacag gctatcgatt aacaatttgc ctttgcggaa catcatcaga
    30481 cgcaggaaca ccaccatcag cgcagtaatg attgccccca gcgcgatccc cgccatataa
    30541 acgcccaggt tagtgaccag cggccatgcc cagatagcgg attccgggaa ccattgcact
    30601 gcgcccagcc agacagctgc ggttgagccg acaatcgcgc cgaccatata tgacggaata
    30661 gcggtgatgg ggctttccag cgcaaacgga atcgcaccct cactaatccc cataaaggcc
    30721 aggaacatcg cggtcttacc ctgcggataa agttgagcgt tgaacaggcg tttgccggtt
    30781 aaacgacggt caataatggt cgccagaccc agaccaatcg gcggaataac gatagcgata
    30841 gaacgagcgg tgaccggtaa tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg
    30901 gctttgttga tcgggccacc aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac
    30961 atcagcgcgc ccttctcacc tgcggcagtc agcacagtac ggataccgcc gttgatccag
    31021 ccaccaaagg gagtgatgac gtagtacatc gccagcatga caaaaatggc ggaaagaatc
    31081 ggtagcaaaa atgtggtttt gaacgctaac aggaaatccg gtaactggat tttctggttc
    31141 atccatttca caaggtaacc ggcaacgata gaaataatca gcgcaccaat gaaggtggac
    31201 ggcaccggcg aagaggtcgc ccattgcata gtgctgggat cgaagttcag cagttgcgtc
    31261 ggctgggtag acatcaaccc gccgataaaa ccagccggga acgccagttt gccaccgata
    31321 gagttcgcca caaatgcagc aaacatcgga atggcaaaac caaacaacac gccgccgaag
    31381 gactgcgaca gccaggcaaa tttaagtaag gagaggtcga aaccggagaa tttcccgcta
    31441 ttaagagcat ccatgatgcc gatctcagca ggaattttaa gccagctata agcaatcagc
    31501 tggctgaaag cgaggatcac accgcccata attaaggtcg gcaccatgcg tgaaatccct
    31561 gacatcacat gctgcgggag ttcaccccag aagctgcttt tagaggcctg cggattctta
    31621 actgccgctg ccgcaccgga tgcgccaggc acaacggttg cactgcgttt tttaatggcc
    31681 ataacaatta tccctgtcga atattactgt tgttcagagg caatcatctc ttcgatttct
    31741 ttgatgatgc ccgcagcgtt tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt
    31801 gattcgaaac gttcgttatc ttccggggta actgccacgg aatggatgat gatggtcgct
    31861 tcggcgatat cctgcgccgt caggcgattc tggatgccgt ccgcgccctg ggtttcaatt
    31921 ttcacttcat aaccggcttc taccgccgct tcttccagca cctgagcggc cataaaagtg
    31981 tgagctaggc ccattgggca ggcacataag gcaatcagtt tcttactcat cgttaattcc
    32041 tcgttcagtg atagttgacg agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag
    32101 acaaataatg catttactgg agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc
    32161 cttgtgaggg gacacaaaga aaaattgaat taactgtatg atttaaaaga ttatcgggag
    32221 agttacctcc cgatataaca ggaaggattt acagaatgtg acctaaggtc tggcgtaaat
    32281 gtgcaccgga accgagaaga cccggatagt catggacgat gagatacacc ggaatatcat
    32341 ggacatattc tttaaagcgc cctttatctt caaatgcggc acggaaaccg gaggctttga
    32401 agaactcaag gaagcgcggc acgataccgc ccgcaataaa cacgccgcca aatgtcccga
    32461 gattgagcgc cagattgccg ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc
    32521 ggcggcaatc ggtgcagctg tcagccagcg cgcgttcggt aatatctttt ggcttgagat
    32581 tttctggcag gcggttgtca gctttcacaa ttgcgcgata caaattcacc agcccagggc
    32641 cagaaagcac gcgctccgcc gaaacatgac caatttccgc acgcaatatt tcgaggataa
    32701 tggcctcttc ttcactattc ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta
    32761 cccaacgctt atcgacatgg accagatgcg caaccccaag ccccgttccg gcaccgtaaa
    32821 ccgcaatagg cttaccttcg accggttctg cgccaccaaa ctgaatcaga tgctcttttt
    32881 tcagcatcgg gatcgccatc gatacagcgg taaaatcgtt aataatttcc agatggctaa
    32941 aaccgagatt ctttttcatt tcggcaattg agaacgccca ggtatggttg gtcattgcca
    33001 cccagtcacc ggtaattggg caagcgatgg caatacagcc gtctttcacc tcgaccttat
    33061 gttcttcaag ataaacgcga atgaccgctt cgaggctggg gtaatcaagc cctgaatagg
    33121 tcttagcctg cgagatttca ccactggcaa tatcacacag agcaagacgt gcgttggtgc
    33181 cgcccacatc accgactaat gcatactttg tcattcttca actgctccgc taaagtcaaa
    33241 ataattcttt ctcacactgt aaatacctgg gggcataaca acaacgccgg aaaggcaggc
    33301 tccctgtaaa tatcgatctg ggtcacacaa ttactttatc gtttcagcac caattgcagc
    33361 gatgcctttt tgcaagctgg gcaaactaag tatctgaccc cgcataagga atagaacatg
    33421 ctccatccgc gagccagaac catgttgtta ttatcgctcc ccgccgtggc aattgggatt
    33481 gcgtccagtc ttattctgat tgtggtgatg aaaatcgcct cggtattaca gaatttgctc
    33541 tggcaacgac tgccgggaac tctggggata gcccaggatt cacccctctg gatcatcggt
    33601 gtattaacgc taacgggtat tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc
    33661 ggaccagacc ccgcctgtga accgctgatc ggcgcaccgg ttccgccctc tgcgctacct
    33721 ggacttatcg tagcattaat tctcggtctt gctggcggcg tcagcctggg gccggaacat
    33781 ccgatcatga ccgtcaatat cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc
    33841 gtcaaccgaa tggagtggac tattttagcc tctgccggaa ccatcggcgc gctgtttggt
    33901 acacctgttg cggcggcgtt gatattttcg caaaccttaa atggcagtag tgaagttccg
    33961 ctatgggatc gtctttttgc gccgttaatg gcggcagcag ctggtgcact tactaccgga
    34021 ttatttttcc atccccattt ttcactgccc attgcgcatt acggacagat ggaaatgacc
    34081 gatattctca gcggtgcaat tgtcgcggcg attgccatcg cagcagggat ggtcgccgta
    34141 tggtgcttac cacggttgca cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg
    34201 ggtattggcg gatttattct cggaattctg ggggttattg gtggaccagt ttcgctgttt
    34261 aaagggctgg atgagatgca gcagatggtg gcaaatcagg ctttcagcac cagcgattac
    34321 tttttgctgg cggtaattaa acttgccgcc ctggtcgttg ctgccgccag tggctttcgc
    34381 ggtgggcgaa tcttcccggc agtgtttgtc ggcgtggcat tagggttgat gctgcatgag
    34441 cacgttcccg ccgttccagc ggcaataacc gtttcctgcg ctattctcgg catcgtgctg
    34501 gtggtaacac gcgatggctg gttaagtctt tttatggcgg cagtcgttgt acccaatacc
    34561 acattgctac cgctgctctg tatcgtcatg cttccggcat ggctgttatt agcaggtaag
    34621 ccgatgatga tggtcaatcg tccgaagcaa cagccacccc acgataatgt ttagcaaaaa
    34681 atgctctcct ttatgattta agagttatgg cgatgatacg gggctttacc tccccgtaat
    34741 attgcgttaa caggccgctg acggcttcga tcgtgaagga gaataacaat gttcaggtca
    34801 ctgtttctgg cggccgccct gatggcattt accccgcttg cagcaaatgc aggtgaaatc
    34861 accctactgc catcgatcaa attacaaatt ggcgatcgcg atcattacgg taattactgg
    34921 gacggtggtc actggcgaga tcgtgattac tggcatcgca attatgaatg gcgcaaaaac
    34981 cgctggtggc gtcatgataa tggctatcat cgtggctggg ataagcgtaa agcatatgag
    35041 cgtggctatc gtgaaggctg gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat
    35101 gggcaccgcc attaaacgcg tcctccaatg gagcacaatg cctgatgcga cgctagcgcg
    35161 tcttatcagg cctacaaacc ggctctttaa cgcgtagggc ggataaggcg tttacgccgc
    35221 atccgccaat agtgccagat gcgacgctca ttcaactaca atcccagcgc cgtccccacc
    35281 aacaaccaga tattcagcgc cacgaccagc actacaatca cccagcctgt ctgttttacg
    35341 cgtttgctgt tcaccagatc gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga
    35401 accagcgcca gggcgatacc aaaacttaac agcacctgac tcataaccag aatccgtgtc
    35461 ggatctaatc ccatcagaat gacaataaat gacggcaaca tggtgactgt acgacgcacc
    35521 cacagcggga tatagaagcg aatgaatccc tgcatcacca cctgccccgc cagtgtcccc
    35581 accaccgttg aggacagtcc ggcagcaacc agacttaacc caaagaccgt tgccgcagca
    35641 tggcttaaca gcggttgcag cgtcagataa gcctcatcaa gatcggcaac accagtatga
    35701 ccagaaaagt ggaacgccgc cgcagctgta gccatcatca ccagattgac aaaaccggca
    35761 atcgtcatgg cgatagccac atcccatttg gtggcggaat aacgttgttg acgcgaaccg
    35821 ccatgtaaat gctgagtgag cgaagagtgc aaataaatca catgcggcat aatcgtcgcc
    35881 cctaacacgc ctgctgccag gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt
    35941 cctttaccca gctgcgccag gttaggctgg gagaaaatca actcgacaat gtaagccgcg
    36001 gcaacaaaca acagtaaccc gccaatcact ttctccagcg gtttttgccc gcgacgttgc
    36061 agcattaaaa tcaggaaagt cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca
    36121 ccaagaatga gtttaaaacc gatcgccgca ccaataaatt ccgccaggtc ggttgccatc
    36181 gcaataattt ctgcctgaac ccaatagaac cacactacgg gacgcggata gtgatcacga
    36241 atctgctccg ccagattttt accggtggca atccctagtt tggcagagag gatctgaatc
    36301 agcatcgcca tcaggttggc ccaaacgaca acccacagta gctgatagcc gaagctagca
    36361 cccgcctgaa tattggtcgc aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac
    36421 gcaggtccca ttaatgcgag cctcatcttg cgcgccgccc gtccgctgct actctcaacg
    36481 cgatagttcg tcatcttgtg cctctaaaac atagcctttg ctatgtttca tgctatgcta
    36541 aacgagaatg attatcaaat tcatttaaat ggattgtggt gatttctctg atagaccaag
    36601 attatgatta cgaaaagatt gatgacttga atgttatggt tatgtttaat gttagcacat
    36661 ttacataact ttcagcttcc atacacaaca tagcagaaat gtatgacaga tcactatttt
    36721 tgaagcctgt cacaggacgt cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt
    36781 tacatagaat gtgcacggaa atttaacctg cctcatattt ggagcaaata tggaccgcgt
    36841 ccttcatttt gtactggcac ttgccgttgt tgtgattctc gcactgctgg taagcagcga
    36901 ccgcaaaaaa attcgtatcc gttatgttat tcaactgctt gttatcgaag tgttactggc
    36961 gtggttcttc ctgaactccg acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt
    37021 cgaaaaactg ctcggatttg ccaacgaagg gactaacttc gtctttggta gcatgaatga
    37081 tcaaggcctg gcattcttct tcctgaaagt gctgtgccca atcgtcttta tctctgcact
    37141 gatcggtatt ctccagcaca ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct
    37201 gctctccaaa gtcaacggta tgggcaaact ggaatccttt aacgccgtca gctccctgat
    37261 tctgggtcag tcagaaaact ttattgccta taaagatatc ctcggcaaaa tctcccgtaa
    37321 tcgtatgtac accatggctg ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc
    37381 atacatgacc atgctggaac cgaaatacgt cgttgctgcg ctggtactga acatgttcag
    37441 cacctttatc gtgctgtcgc tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat
    37501 ccagatgtcc aacctgcacg aaggtcagag cttcttcgaa atgctgggtg aatacattct
    37561 ggcaggtttc aaagttgcca ttatcgttgc cgcgatgctg atcggcttta tcgccctgat
    37621 cgccgcgctg aacgcactgt ttgccaccgt tactggctgg tttggctaca gcatctcctt
    37681 ccagggcatc ctgggctaca tcttctatcc gattgcatgg gtgatgggtg ttccttccag
    37741 tgaagcactg caagtgggca gtatcatggc gaccaaactg gtttccaacg agttcgttgc
    37801 gatgatggat ctgcagaaaa ttgcttccac gctctctccg cgtgctgaag gcatcatctc
    37861 tgtgttccta gtttccttcg ctaacttctc ttcaatcggg attatcgcag gtgcagttaa
    37921 aggcctgaat gaagagcaag gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg
    37981 ctctaccctg gtgagtgtgc tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac
    38041 ataaaaaagc cggggatcat tccccggctt ttttacgcct gttaatcagc taatggctgc
    38101 gggcgaccaa tcaaataccc ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc
    38161 tgctgttgcg tctcgacaaa ctccgcgacc acactcaatg acttcgcttt cgccagatcg
    38221 gtaattgatc tcacaatcat cgcatccagc gtattcgtga caatatcttt cacaaagacg
    38281 ccatcaattt tgatgatatc agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg
    38341 gtgccaaagt catcaatcgc aatccggaaa ccaaacttat gcagctgctc gatgttgtac
    38401 atgctgcttt ctgcgttaga aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc
    38461 ggggagatgt gataacgctt aaacagacga ataatccgcc cggcaatatt cttttgcagc
    38521 agcgtgagcg gcattaaatt gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt
    38581 gccagccact tcaacaagga ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac
    38641 tgagcaataa ggggcagaaa cttatccggg gtcataatgc cgccgtcata tttcagtcgc
    38701 gcgaggatct catcataacc ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc
    38761 aaatcaccct gatctaacgc cgtgcgaatg gtattcagca atagcacctg tttagtggtc
    38821 tgcccggaaa ccatctcctc tctgctatcc agcgccagca catgatgatg tgcgcaggat
    38881 tgctccgcca gccagcttaa ctgccccaac aagggttgca gggtttcctg atttccatca
    38941 aaacgccccc aggcagcacc atagcccata tccagcccgg tattgttcca gtgaatttgc
    39001 cgactattca ggatgttaac catatgctgg agtcgccctt ccgtttccgg cccgctcagc
    39061 actaacagca gttcactacc cggcaattga tacaactttt cgttttcctg catcaacggc
    39121 agcagcgtac ggcaaattga gcggatacag tgaacgcgca tcattaagcc gtaatgacga
    39181 ctcataaact caagattatc aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc
    39241 ggcgcttgtt ccaacgcacg aaagttgggt aggagcgtta acggatctgt tagcgcctgc
    39301 aaatgccagc ggcgattaag ccattcactg cgatgataaa tgcgcaccat atagagcagg
    39361 caaacgctaa aggaaatcag caccgcgaga ataaatgcca gcgaatattc ggtttccacc
    39421 ccttgcaaaa agttctggtt gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga
    39481 tttaaaaacg gatagcgaag attaccgacc ccgagggtaa agatgatgaa gaaaacgggt
    39541 accaggtaac cggcaataaa gtcgttttca taaggtgtgc acaacagaag tagcagcacg
    39601 cttaacgctg ccagccagct taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta
    39661 aatcagcact gcggtgaaaa ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc
    39721 gccgaaaaag gtggatatct ttagcgggaa atcaaagaag ctccccacaa gatacatgct
    39781 gcatttgatg ccgatcggcg tcaccaaacc aagccagaac agacgttgcc agacatatcg
    39841 gctggtcaat ccataacgcc aacgcgtccc cagctgccag cgtaatattg cgcaggcaca
    39901 caacacgaca aacgtctggc tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa
    39961 gttccacaga ttggtgcaaa acatcccgca taaaacggga acgacaccgc gccagccaaa
    40021 gataaacaag attgacagca ttacgcacag tggcatccac gccagaaaga tataactgga
    40081 atggataatg gcaagtggcg aaataaaacg ggatagctga ataagtacca cggtgagcgt
    40141 aaacgctagt gtgaatatct tgatattttt tatcaggtta tgctccacaa acatgagtac
    40201 actactctca ctgatcgggg ttttcaggca cacaaataat aagtttattc gcatcgcgaa
    40261 gcaaacaggg gccagcctgt tgccgatttt tcatccaatg atgctttcac caggcggcgc
    40321 taacccggac gtagactgta ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct
    40381 ttgcagcgag ggttggaaat ttggtggagc taagcgggat cgaaccgctg acctcttgca
    40441 tgccatgcaa gcgctctccc agctgagcta tagccccacg atgcgtttac gtaccaaatt
    40501 tgctgggtgc aaaatttggt ggagctaagc gggatcgaac cgctgacctc ttgcatgcca
    40561 tgcaagcgct ctcccagctg agctatagcc ccgtcacgta aagcttgtcg agttgacggg
    40621 cggcatcata tgaattccgc ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat
    40681 cgctgaaaaa tcaggcaaat gaacaatttt ggaaaaccgc tcgcattcag tagttattca
    40741 tgtcacggtt tcctgtaaag tggtgttata aaatgaacta ctaatagacc cacatacatt
    40801 cagggaattg ttatgttcaa ggagaggatg acaccagatg aacttgccag actgaccggc
    40861 tatagccgcc agaccattaa taaatgggta cgcaaggaag gctggacaac gtcaccaaaa
    40921 cctggcgtcc agggtggcaa agcccgactg gttcacgtca atgaacaggt tcgtgaatat
    40981 attcgcaatg ctgaacgtcc agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg
    41041 ccgcttgaag tattgctggt gacactggcg aaagagatga cgccagttga acaaaaacag
    41101 tttacatcct tgcttctgcg ggaagggatt atcggattgt tacaacgctt agggattcgc
    41161 gatagcaaat aatatgaaaa gattacgcaa taaaatgacc accgaagaac tggctgaatg
    41221 cctcggtgtg gctaaacaaa ctgttaaccg ttggaccaga gaaaaaggct ggaaaacgga
    41281 aaaatttccc ggcgtgaaag gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg
    41341 cgagtttatt cagaacacgc cagccttcca taacacacca atgttgatgg aagctgaaga
    41401 gagaattgct gaatacgctc ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc
    41461 gatagataat atgacagaca ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg
    41521 aattcgcaat ttcctcgccc gtctcgatat tgacgaatca gcataaataa aaaacggcag
    41581 gatattatct cctgccgttt atctttttac acgctaatta ctgctgattt tcgcgttcag
    41641 caataaaatc cagcgctttg ttgatacgct cgatactgcg ggtcttacca attgcgtgaa
    41701 cggtaacatc cagtgctgga gactgccccg caccggttac ggcgacacgc agcggcatac
    41761 caactttacc catacccact tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt
    41821 tttcagcggt ccagtcagta atcgcggcca gtttgtcacg aaccacttcc agcggctgac
    41881 gcgctaccgg acgcagatgt tttttcgccg catcggcatc gaactcagca aaatcttcgt
    41941 agaaataacg gcagctctgt gccatctctt tcagcgtctt gcagcgttcg cccagcagtt
    42001 tcaccagatc agccagctgc gggccgttac gggtatcgat attttcctgc tcaatgtgcc
    42061 actgtaagtg agtagcaaca tactccggcg gcagcgcgtt aatgtagtga tggttcagcc
    42121 acagcagctt gtcggtgttg aacgcactgg cagatttgct gacggcattc aaagtgaagt
    42181 atttgatcat ctcttcacga gtgaagattt cctgatcgcc gtgggaccag cccagacgca
    42241 ccagatagtt cagcagtgct tctggcaaat aaccgtcatc acgatactgc attacgctga
    42301 ctgccccgtg acgtttggac agttttttac cgtcatcgcc attgatcata gaaacgtgcg
    42361 cgtaaaccgg caccggcgct ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt
    42421 tgatatggtc ttcgccacgg ataacgtggg tgatttccat atcccagtca tcgacaacca
    42481 cacagaagtt ataggttggg gaaccatcgg tacggcggat aataagatcg tccagttcct
    42541 ggttgctgaa ctcgatcgga ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg
    42601 ggttagcaaa acgtacaaca cacggttcat catcagcatg atgctcatgg ctgtggcggc
    42661 agcgaccgtc ataacgcggc ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca
    42721 ggcgctcttt agagcaatag catttataag cagtgccctc ttccagcatc tgatcgatca
    42781 ccgcgttgta gcgatcaaaa cgtttggtct ggtagtacgg accttcatcc cactccaggc
    42841 tcagccagtt catgccatcc ataatggctt cgatagcttc cggcgtggaa cgctcaagat
    42901 cggtgtcttc aatacgcagc acgaactcac cgccgtggtt acgtgcaaaa agccaggagt
    42961 aaagagcagt acgcgcgccg ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag
    43021 ttttgatttt catgaaatgg ccttacgttt agaaagatgc cgacaaccgg caaatcctgg
    43081 aaaaattgag tgggcgatat tctatcactc ccgcctgatt cctcaatgta gttcgggttt
    43141 ctaccttacg cattattgtt ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca
    43201 tcttgcttaa atttacgacg aacgaacaat ttctttagaa aatgcgttga ctcattttga
    43261 actctcccta taatgcgact ccacacagcg ggggtgatta gctcagctgg gagagcacct
    43321 cccttacaag gagggggtcg gcggttcgat cccgtcatca cccaccaact actttatgta
    43381 gtctccgccg tgtagcaaga aattgagaag tgggtgatta gctcagctgg gagagcacct
    43441 cccttacaag gagggggtcg gcggttcgat accgtcatca cccaccactt cgggtcgtta
    43501 gctcagttgg tagagcagtt gacttttaat caattggtcg caggttcgaa tcctgcacga
    43561 cccaccaatg taaaaaagcg ccctaaaggc gcttttttgc tatctgcgat actcaaagat
    43621 tcgaacctgc agcaggtttg agttgagcgc agcgaaacaa cggagccgct cgcggcgacg
    43681 gcccgaaggg cgagcgaagc gagtcatcct gcacgaccca ccaatgcaaa aaagcgccct
    43741 aaaggcgctt ttttgctatc tgcgatactc aaagattcga acctgcagca ggtttgagtt
    43801 gagcgcagcg aaacaacgga gccgctcgcg gcgacggccc gaaaggcgag cgaagcgagt
    43861 catcctgcac gacccaccaa tgcaaaaaag cgccctaaag gcgctttttt gctatctgcg
    43921 atttgtaaga ttgatttttc ttctcaagtt ccttgttctt ctaccgatat tacctcgtac
    43981 agtgacgagg actgaacaat ggccactatc tcttcaactt caattccttc catacaaaca
    44041 cagagtagta acagggcatc acaaggtagc gatgttgctt cacaaatcgc gcggataagt
    44101 cagcaaatca tcaaactcac tcaacaaatt aaagagattg tagacactag cggcagtgct
    44161 gaagacaagc aaaaacaggc cgaattgatt caacagcaaa tcaccctact ggaaacacaa
    44221 ctggcgcagt tgcagaaaca gcaggcagaa aaggcgcagg agaaagagca gcgtttgtct
    44281 ctgaatgtat cccttctaaa tcctgttgaa aaaacgactc atatcgatat ctatatctga
    44341 tacaacggat ccccttcccg accagccatt agcgcattgc cctggtctcg taccacgtcc
    44401 cataacgctt cagcggcggt ggaaagtgaa cggttcttcc tgcgcaccag catcaactgg
    44461 cgttcaacca ccggcgtaat gcgcttaacc accagtgggc taccttcagg caatggtagc
    44521 gccagtgcag gaagaatact gatgccgatg cctgcggcta ccatcgggaa cagcgtcgcc
    44581 ggatgtccaa tctcctgcac aatattcgcc tgaataccat tgcgcgccag cgctgcgtca
    44641 atcagcggtc ggctgcctga cgcgtaatcc tgcaacacca atttcgctcc ctgtaatgcc
    44701 tgccagggta cataatcttc cacggccaaa gcgctatcgc gatggcaaag cagaaagaaa
    44761 ggctcggaaa gaatcgcttc acattgcaga tcgcccactg ggcctggatc gatgacgatg
    44821 ccaaagtcga catctccctg gcgaatactt tccatcaccc actgctgcgg acgatcatgc
    44881 aggacaaact gaatatctgg atagcggcga tggctttcag caatgcattg cggaataaga
    44941 tgcgcggaaa tggtctggct ggcagcgacc cgaacttttc cgctcagttg ttgccccata
    45001 cgcccggtat cgcgcaacgt gctgttaagt tcatccagca gtcgctcaag acgcaaagcc
    45061 aactgctgcc ctgcatctgt aagcaccact tcacgcgtgg ttctgtccag caagcgaaca
    45121 ccagtatgat tttccagctc cttcacactg tgacttactg ccgactggct caagccgata
    45181 cgctctcctg cacgactaaa acttttctcc tgcgctactg tgacgaaaac ctttaattgt
    45241 tttaaagaat aattcatctg ttttcttcat gaatggatgc aataaatcaa ttttatttct
    45301 caaactggaa gaagcacaat agaaccatcg atcatctgga gtctttatga aactttttcg
    45361 tatcctcgat cctttcacct taaccctgat cacggtggtg ttgctggcct ctttctttcc
    45421 ggccagaggc gatttcgtcc ccttctttga aaatctgacc accgcagcta ttgccctgct
    45481 gttctttatg cacggcgcga agttgtcgcg tgaggcgatt attgctggcg gtggtcactg
    45541 gcgactgcat ttgtgggtaa tgtgcagcac cttcgtgctg tttccgattc tgggtgtact
    45601 gtttgcctgg tggaaaccgg taaatgtcga cccgatgctc tactccggtt ttctctactt
    45661 gtgcattctc ccggctaccg tgcagtctgc aatcgccttc acgtcaatgg cgggcggtaa
    45721 cgtcgcggcg gcggtttgtt ctgcgtcagc atccagcctg ctggggattt tcctttcacc
    45781 attgctggtt ggtctggtga tgaatgttca cggtgcaagg ggcagccttg agcaggtcgg
    45841 taaaattatg ctgcaactgc tgctgccgtt tgtgttgggg catctttccc ggccgtggat
    45901 tggtgactgg gtgtcgcgca ataaaaaatg gattgcgaaa actgaccaga cgtccattct
    45961 gttggtggtt tatacagcgt tcagcgaagc cgtcgttaat ggtatctggc ataaagttgg
    46021 ctggggatca ttgctgttta tcgtggtggt cagctgcgtt cttctggcta tcgtgattgt
    46081 agttaacgtc tttatggcac gccgactgag cttcaataag gcagatgaaa ttactatcgt
    46141 cttttgtggt tcgaaaaaga gtctggcaaa tggcatcccg atggcaaaca ttctgttccc
    46201 cacatcggtg atcggtatga tggtgctgcc cctgatgatt ttccatcaga tccaattgat
    46261 ggtctgtgcg gtgctggcgc gtcgatacaa acgccagacc gaacagttac aggcgcagca
    46321 ggaaagcagc gccgataaag cttaaagcgg acgcttcagg ggctggacca gttgcgtcag
    46381 cccctcggtt ttaatcagca gcgtgatttg cattagctca ccgagcttcc ctgccgggaa
    46441 ctcatctttg cgggcaaacc acagcagata ctcctccggc aggtcaatta agcgacgccc
    46501 tttgtatttg ccaaacggca ttatcgtatt ggctatttca atcagctgct ctttttccat
    46561 ctcagctacc cagcaaacgc agcatttccg cttcgtcgat gacttcaatg cccagttcct
    46621 gcgccttcgc cagtttagat cctgcagctt cacccgctat caccagatcg gttttcttcg
    46681 acacgctgcc cgcgactttc gcgcccagtt cgaccagtcg agctttagcg tcatcacgcg
    46741 acatctggct taagctgccc gtaagcacca cggttttacc agcaaacggg ctgtcaatct
    46801 cttccgcgtt gataacgatc ggcgcaggcc agtgaacacc ttccgccaac agctcgctga
    46861 tgacattgcg gttgctttct tcggcaaaga agttgtgaac gtgggatgca acgacaatgc
    46921 caacatcagg caccttttgc agctcttcaa tcgaagcggc ttccagcgct tccagcgtgc
    46981 cgaaatatgc cgccagacct gctgcggtgg cctcgccgac ttcacggatg ccaagtgcat
    47041 agaggaagcg agcaaaggtg gtttctttcg ctttttccag cgcgttaacc acgttttgtg
    47101 ccaattttgg ccccatacgc tccagtccgg tcagtttgcc tgcggtgagt ttgaacagat
    47161 ctgccggagt atggacatat tctttttcaa ccagctgatc gatgattttg tcgcccattc
    47221 cgtcaacatc catcgcacgg cgggaaacaa agtgtttcag cgactcttta cgctgcgcac
    47281 cgcaaatcag gccaccggta cagcgggcaa ccgcttcacc ttccacacgc tcaacgtcag
    47341 aaccacatac cggacaatgc gtcgggaata caacctcacg ggtatcttcc gggcgttcag
    47401 aaagcacgac gttaaccacc tgcgggatca cgtcgccagc gcggcgaatc accactttat
    47461 cgccaatgcg taaaccaaga cgttcgattt catccgcatt gtgtaaggtt gcgttactca
    47521 ccagcacgcc agcaacatgg acaggttcca gacgcgcaac aggcgtaatc gcgccagtac
    47581 gcccaacctg aaactcgacg tcacgcacaa aggtcatctg ctcctgcgcc gggaatttaa
    47641 acgcaaccgc ccagcgcggg gcacgcgcga caaagccaag ctgctcctgc tgttccagtg
    47701 agttgacctt aatcaccacg ccgtcgatat caaagcccag cgtcgggcgg tcttcttcca
    47761 ctttgtgata gaacgccagc acttcttccg ccgattcaca aagcgttacc cgatcgctga
    47821 ccggcacccc ccacttttta aattgcagta aacggccaag atgagtatcc ggcagctcgc
    47881 caccttccag aacaccaacg ccatagcaga aaaaagtgag cggtcgcttc gctgtaatac
    47941 gcggatcaag ctgacgcagt gaaccagctg ccgcattacg tgggttagca aaaactttcc
    48001 cgcccgtgcg tcgcgcatct tcgttaatct tttcgaaccc cgcctgcggc aggaacactt
    48061 caccacgcac ttccagacgc gccgggatat tctctccgtg cagcttcagc ggaatggcgc
    48121 gaatagtacg cacattagac gtgatatctt ccccggtggt gccatcgcca cgggtcgcgg
    48181 cactgactaa aacgccattt tcatacagaa tactgacggc aaggccatcc agcttcagct
    48241 cacagcacca ggtgactttc tcgttgtttt tcagacggtc ctgcacacgt ttgttgaaag
    48301 caagaaagct ttcttcatca aaaacgttat ccagtgacag cattggtact tcatggcgta
    48361 tctggctgaa agctgccagc ggcgcagcac ctacacgttg ggtaggcgaa tcaggcgtaa
    48421 tcagttctgg atgtttggtt tccagctcgc gcagttcgcg catcagcctg tcgtattcag
    48481 cgtcgggaat ttccggcgca tccatcacat gataaagata ttcatgatgg caaagcgtcg
    48541 ttcgcagttc tgtcagttgt tgttcgattg attccatatc gcaccatcaa tgctaaaaac
    48601 ccccgacaag cgggggttcg aagaggagtt aatttgcctt aagtgtatca ggcgttggcg
    48661 tctttgactt cgcggatgat gtcctggtac tcgcgcaatt tctgcggagt catcatacgg
    48721 cgctgatcgt caagcacgac accgcccact tcatcggcaa tatgctgcgc agattgcagc
    48781 atcagcttga agttctgcag ctcgtcaccg taagacggta cctgcataaa gatagtgaca
    48841 cccggagtag tgaaatcctt catttcagga tcaaaggttc ccggtttcac catattcgcc
    48901 aggctgaata acgccgggcc gctgccatcc gggctaagat gacgatggta aatattcata
    48961 tcgccaaaaa tgaagcccgc ttgttgaatg ctgttaagaa gcagttcacc gtttagctcg
    49021 ctaccgtgat gcgccgcgac gttcatgata atcaccgctt ctttgcgctt cggtttatcc
    49081 ataactggag ccggttccgc tacaggctca ggctgtggtg ccgctacggg ttctgcaggc
    49141 tggaaagcct gttgtgccgg ttgcggtgct gaatgcacag gctgcggcgc tggcgcgacc
    49201 tgtggcgaaa ctggctgctg caacggctgt tcaggctgcg gctgataggc aggctgctgc
    49261 accggctgcg ctggacgcgg agcatgttgc ggcggtacct gcgcttcagg cggctgctgg
    49321 accggttgac gcggctgcgc agacgcataa ggcggtaggt actggtgttg cggcgacgga
    49381 cgagcagcct catgctcctg agcgttagcc ggggcatgat tcacgcggtg aacacgaacc
    49441 tcaccaacgc cctcatcatc ttcgacatcc tcgtcataag aatcgtcgtc acgttttgac
    49501 ttcattcgtt ttaatggccg atcgcggaac atagaagatc gttctttacg gctggtccag
    49561 aaaccatgta ccagtaaagc gattatggcg atcgcgccaa caatgattaa tatcagacgc
    49621 aaatcctgca tcattatatt ctctgttgtt ctaacacctt gccaccacgg caaacattta
    49681 ctcactaaga gtatttgccg attacctcaa gtgcaagtgc actattaact ttcacagcac
    49741 aaagatagat gaaatcgtgc tttttgctgt tttttcgaac atatcctaac tgtccattgc
    49801 gcaattaccc ggtaaaatac gcagaatttt cctgggattg gtcaaaagga gctcatcctg
    49861 attatggttt catcattcac atctgcccca cgcagcggtt tttactattt tgcgcaaggc
    49921 tggaagctcg tctcgcaacc tgggattcgg cgtttcgtta ttttaccgct gctggtcaat
    49981 attttgttga tggggggcgc attctggtgg ctctttacac agctcgatgt ctggatcccg
    50041 actttcatga gttacgttcc ggactggcta caatggctga gttatctgtt gtggcctctg
    50101 gctgtcatct ctgtgctgtt agtgtttggc tatttcttct ccacgattgc taactggatt
    50161 gccgctccgt ttaacggttt attggctgaa caactggaag cacgattgac tggcgctaca
    50221 ccgccagata ccgggatttt cggtatcatg aaagatgtgc cgcgaatcat gaaacgcgaa
    50281 tggcaaaaat ttgtctggta tctgccgcgc gcaattgtat tactaattct ttacttcatc
    50341 cctggtattg ggcaaaccgt cgcgccggta ctgtggttcc tgtttagcgc ctggatgtta
    50401 gccatccagt attgcgatta ccccttcgat aaccacaaag tgccgtttaa agagatgcgc
    50461 accgccctgc gcacacgcaa aatcaccaat atgcagtttg gtgctttaac cagcctgttt
    50521 acgatgatcc cgctgcttaa tctgttcatc atgcccgttg ccgtttgtgg cgcgacggcg
    50581 atgtgggtcg attgctatcg cgataaacac gcgatgtggc ggtaacaatc taccggttat
    50641 tttgtaaacc gtttgtgtga aacaggggtg gcttatgccg ccccttattc catcttgcat
    50701 gtcattattt cccttctgta tatagatatg ctaaatcctt acttccgcat attctctgag
    50761 cgggtatgct acctgttgta tcccaatttc atacagttaa ggacaggcca tgagtaagat
    50821 ttttgaagat aactcgctga ctatcggtca cacgccgctg gttcgcctga atcgcatcgg
    50881 taacggacgc attctggcga aggtggaatc tcgtaacccc agcttcagcg ttaagtgccg
    50941 tatcggtgcc aacatgattt gggatgccga aaagcgcggc gtgctgaaac caggcgttga
    51001 actggttgaa ccgaccagcg gtaataccgg gattgcactg gcctatgtag ctgccgctcg
    51061 cggttacaaa ctcaccctga ccatgccaga aaccatgagt attgaacgcc gcaagctgct
    51121 gaaagcgtta ggtgcaaacc tggtgctgac ggaaggtgct aaaggcatga aaggcgcaat
    51181 ccaaaaagca gaagaaattg tcgccagcaa tccagagaaa tacctgctgc tgcaacaatt
    51241 cagcaatccg gcaaaccctg aaattcacga aaaaaccacc ggtccggaaa tctgggaaga
    51301 taccgacggt caggttgatg tatttattgc tggcgttggg actggcggta cgctgactgg
    51361 cgtcagccgc tacattaaag gcaccaaagg caagaccgat cttatctctg tcgccgttga
    51421 gccaaccgat tctccagtta tcgcccaggt gctggcaggt gaagagatta aacctggccc
    51481 gcataaaatt cagggtattg gcgctggttt tatcccggct aacctcgatc tcaagctggt
    51541 cgataaagtc attggcatca ccaatgaaga agccatttct accgcccgtc gtctaatgga
    51601 agaagaaggt attcttgcag gtatctcttc tggagcagct gttgccgcag cgttgaaact
    51661 acaagaagat gaaagcttta ccaacaagaa tattgtggtt attctaccat catcgggtga
    51721 gcgttattta agcaccgcat tgtttgccga tctcttcact gagaaagaat tgcaacagta
    51781 atgccagctt gttaaaaatg cgtaaaaaag caccttttta ggtgcttttt tgtggcctgc
    51841 ttcaaacttt cgcccctcct ggcattgatt cagcctgtcg gaactggtat ttaaccagac
    51901 taattatttt gatgcgcgaa attaatcgtt acaggaaaag ccaaagctga atcgatttta
    51961 tgatttggtt caattcttcc tttagcggca taatgcttaa tgacgtacga aacgtcagcg
    52021 gtcaacaccc gccagcaatg gactgtattg cgctcttcgt gcgtcgcgtc tgttaaaaac
    52081 tggcgctaac aatacaggct aaagtcgaac cgccaggcta gactttagtt ccacaacact
    52141 aaacctataa gttggggaaa tacaatgttc cagcaagaag ttaccattac cgctccgaac
    52201 ggtctgcaca cccgccctgc tgcccagttt gtaaaagaag ctaagggctt cacttctgaa
    52261 attactgtga cttccaacgg caaaagcgcc agcgcgaaaa gcctgtttaa actgcagact
    52321 ctgggcctga ctcaaggtac cgttgtgact atctccgcag aaggcgaaga cgagcagaaa
    52381 gcggttgaac atctggttaa actgatggcg gaactcgagt aatttcccgg gttcttttaa
    52441 aaatcagtca caagtaaggt agggttatga tttcaggcat tttagcatcc ccgggtatcg
    52501 ctttcggtaa agctctgctt ctgaaagaag acgaaattgt cattgaccgg aaaaaaattt
    52561 ctgccgacca ggttgatcag gaagttgaac gttttctgag cggtcgtgcc aaggcatcag
    52621 cccagctgga aacgatcaaa acgaaagctg gtgaaacgtt cggtgaagaa aaagaagcca
    52681 tctttgaagg gcatattatg ctgctcgaag atgaggagct ggagcaggaa atcatagccc
    52741 tgattaaaga taaacacatg acagctgacg cagctgctca tgaagttatc gaaggtcagg
    52801 cttctgccct ggaagagctg gatgatgaat acctgaaaga acgtgcggct gacgtacgtg
    52861 atatcggtaa gcgcctgctg cgcaacatcc tgggcctgaa gattatcgac ctgagcgcca
    52921 ttcaggatga agtcattctg gttgccgctg acctgacgcc gtccgaaacc gcacagctga
    52981 acctgaagaa ggtgctgggt ttcatcaccg acgcgggtgg ccgtacttcc cacacctcta
    53041 tcatggcgcg ttctctggaa ctgcctgcta tcgtgggtac cggtagcgtc acctctcagg
    53101 tgaaaaatga cgactatctg attctggatg ccgtaaataa tcaggtttac gtcaatccaa
    53161 ccaacgaagt tattgataaa atgcgcgctg ttcaggagca agtggcttct gaaaaagcag
    53221 agcttgctaa actgaaagat ctgccagcta ttacgctgga cggtcaccag gtagaagtat
    53281 gcgctaacat tggtacggtt cgtgacgttg aaggtgcaga gcgtaacggc gctgaaggcg
    53341 ttggtctgta tcgtactgag ttcctgttca tggaccgcga cgcgctgccc actgaagaag
    53401 aacagtttgc tgcttacaaa gcagtggctg aagcgtgtgg ctcgcaggcg gttatcgttc
    53461 gtaccatgga catcggcggc gacaaagagc tgccatacat gaacttcccg aaagaagaga
    53521 acccgttcct cggctggcgc gctatccgta tcgcgatgga tcgtaaagag atcctgcgcg
    53581 atcagctccg cgctatcctg cgtgcctcgg ctttcggtaa attgcgcatt atgttcccga
    53641 tgatcatctc tgttgaagaa gtgcgtgcac tgcgcaaaga gatcgaaatc tacaaacagg
    53701 aactgcgcga cgaaggtaaa gcgtttgacg agtcaattga aatcggcgta atggtggaaa
    53761 caccggctgc cgcaacaatt gcacgtcatt tagccaaaga agttgatttc tttagtatcg
    53821 gcaccaatga tttaacgcag tacactctgg cagttgaccg tggtaatgat atgatttcac
    53881 acctttacca gccaatgtca ccgtccgtgc tgaacttgat caagcaagtt attgatgctt
    53941 ctcatgctga aggcaaatgg actggcatgt gtggtgagct tgctggcgat gaacgtgcta
    54001 cacttctgtt gctggggatg ggtctggacg aattctctat gagcgccatt tctatcccgc
    54061 gcattaagaa gattatccgt aacacgaact tcgaagatgc gaaggtgtta gcagagcagg
    54121 ctcttgctca accgacaacg gacgagttaa tgacgctggt taacaagttc attgaagaaa
    54181 aaacaatctg ctaatccacg agatgcggcc caatttactg cttaggagaa gatcatgggt
    54241 ttgttcgata aactgaaatc tctggtttcc gacgacaaaa aggataccgg aactattgag
    54301 atcattgctc cgctctctgg cgagatcgtc aatatcgaag acgtgccgga tgtcgttttt
    54361 gcggaaaaaa tcgttggtga tggtattgct atcaaaccaa cgggtaacaa aatggtcgcg
    54421 cctgtagacg gtaccattgg taaaatcttt gaaaccaatc acgcgttctc tatcgaatct
    54481 gatagcggcg ttgaactgtt cgtccacttc ggtatcgaca ccgttgaact gaaaggcgaa
    54541 ggcttcaagc gtattgctga agaaggtcag cgcgtgaaag ttggcgatac tgtcattgaa
    54601 tttgatctgc cgctgctgga agagaaagcc aagtctaccc tgactccggt tgttatctcc
    54661 aacatggacg aaatcaaaga actgatcaaa ctgtccggta gcgtaaccgt gggtgaaacc
    54721 ccggttatcc gcatcaagaa gtaattcttg ccgcagtgaa aaatggcgcc catcggcgcc
    54781 attttttatg cttccgccag cggcggcaaa atcaattcat cgctctcatg ctgctgggtg
    54841 tagcgcatca cttccagtac gcgcaacccc gcacggtgca ccgcatcgtt taacgccttc
    54901 cctttcagca aaccactgat aagctgagca caaaacaggt cgccagtccc tttcaggtcg
    54961 gtttttaccc gtgaatggga aatgacattc acgctgtcgg cactgaccac caccacctgc
    55021 atctcctgat tttcttcatt accggaggcg ctggtaatca ccacccattt taatgtgtct
    55081 gaaagcagac tttttgcggc agcaatggca ctgtcgagat cgcggcaatt tttaccggtc
    55141 aggatttcca gctcaaagat attgggggta attccctgcg ccagcggcag taaatattgt
    55201 cgatacgcct cgggaaggtc aggtttgaca taaattccgc tatcaatatc gccaatcacc
    55261 ggatcgacca tgatcaatag gtcaggatgg tctttgcgta gcgcagtcag ccactcggca
    55321 aggattttga tttgcgatgc cgttcccata tagcccgtgg ttacagcacg aagttggcgc
    55381 agcgcatcac gctcctgaag cgcacgcaaa tagccgctaa accattcgtc cggaatcgca
    55441 ccaccgtaga aagtgtcata atgcggcgta ttgctcagca ataccgtcgg cacggcaaag
    55501 acattcaagc cgttctgttt tatagcaggc accgcaatgc tgttgcctac gcttccgtac
    55561 actacctgcg actgcacggc gacgatatcc gcctgcagcg ccctactctt atcgttaaac
    55621 aacaacaaac tactcattta attttttctc cttgccgatg atcctcatcg tattccaacc
    55681 gaaactttac ctgattctgg cagtcaaatc ggctattaca aaacaaagat aaggtaattc
    55741 agtgaagaaa atcatttgtc tggccattac gctgctaatg acactccccg cttacgcgaa
    55801 gttaactgtt catgaagaag cccgcatcaa cgccatgctg gagggattag cacagaaaaa
    55861 ggatttgata tttgtgcgca acggtgatga acatacctgc gatgaagcgg tttcccacct
    55921 gcgtctgaag ctcggcaata cccgtaaccg cattgacact gccgagcagt ttattgataa
    55981 ggtagcttcg tcgtcatcga ttaccgggaa gccgtatatc gtgaagatcc ccggtaagag
    56041 cgatgagaac gcacagcctt ttttacatgc gttaattgcg cagacggata aaacggtgcc
    56101 tgcggaagga aattaatccg ctttggtaag gcatttacag gaggtaacat gaaaaaatgc
    56161 tttatttatc acgatgaaaa atcgaataaa ttttggtgga tagattacga aggggatagt
    56221 ttagctgtca actatggcaa ggtaggtagt attggtaaat tccatacaaa agaattcgat
    56281 aatgaagaac agtgtctgaa agaagccagt aaattgattg ccgcaaaaat gaaaaaagga
    56341 tatcaagaaa ctccagactt taacttcatg gatctctact attttgatga tgaagagatt
    56401 gggttacatc ttaaaacgtc acacccaaac ttccagtgcc attttactga tccactttat
    56461 atgtgttgct gggatgaaga atctcctttt ggtagcgatg aaggtgctga tgctctaaac
    56521 gaacttgaaa atagcctccg ttctggactg tgctgatttc cctcaaatgt taattgaaac
    56581 tatgtggggt atgaaataca tcgctatgga cagtattctt gaagaggatg tgcgtgcgca
    56641 attactagcc gatgaaatga gctctatcca gagcaatatg attacctacg cgactgcatt
    56701 cggtcagatc aaagtcatgg gtaaaatctc ccataaactt aaaaagatgg gactcaatgc
    56761 actagcgcgt catcagctta ccgcaaaaat tcttcaatgg ggtgacggtc aggactcacc
    56821 aatacttcaa aaaatgattg atgaccttac tgcttttcca cacgaaaatt aaaaactgca
    56881 tttgtcggca gcaacaactg ccgacaaatc agattattga caaagtgcac tttgtttatg
    56941 ccggatgcgg cgtaaacgcc tgatccggca taaaaatatt gctaattcaa tatattgcag
    57001 aaacattgta ggcctgataa gcggagcgca tcaggcagtt ttgcgtttgt catcagtctc
    57061 caatgctatt aatccttaaa tccctgcccc ctgactaaaa tgctcttccc caaacactcc
    57121 ggtggagagg tagcgatcgc cacgatcgca gatgatcgct accaccaccg caccagggtt
    57181 agcttttgcc acccgcagtg ctccagcaac cgcgccgcct gagctgacgc cacagaatat
    57241 tccttcccgc accgccagtt cgcgcatggt gttttccgca tcgcgctgat gaatatccag
    57301 cacctcatcc accagcgcag cgttgaaaat ccccggcaga tattccgcag gccagcggcg
    57361 aatgcccgga atactgctgc cttcttccgg ttgcaggcca acaatggtca ccggtttgga
    57421 ttgttcgcgc ataaagcatg aaacgccggt gatagtgccg gtcgtcccca tgctggagac
    57481 aaaatgagtg atgcgcccgc cggtttgctg ccagatttcc ggcccggtgg tggtgtaatg
    57541 cgcataaggg ttatcgggat tattgaactg atcgagcagc tttccttcgc cacgattcgc
    57601 catctccagc gccagatcgc gcgcaccttc catgccctgc tctttggtga caagaatcag
    57661 ttccgcacca taagcacgca tcgccgcacg gcgttcctgg ctcatgttgt cgggcatcag
    57721 caatttcatg cgatagcctt tcagcgcggc aatcattgcc agcgcaatgc cggtgttacc
    57781 actggtggct tcgattaaga catcacccgg tttaatttcc ccgcgctttt ccgcctcgac
    57841 gatcatcgaa agtgccgcac gatctttcac cgaacctgcc gggttattgc cttccagttt
    57901 taaccacact tcactgccgt tatccggccc cattcgctgc aacttcacca gaggcgtatt
    57961 gcctattgtt tgttctaatg tactcacaat ctctatccat acgtggtgtt gcctgatgcg
    58021 acgcttgcgc gtcttatcag gcctacaggt tacaaacctt gccattaaaa agcccggatc
    58081 gcgggaagcg cctccgggcg tttaacattc actcaaccta tcaggcgctt tgtgcgagag
    58141 caagttcctc atcgcgggtt tcaatacgct cgtcgccgtt atacagccgc gcatgttgca
    58201 gaccaacgaa taaacgctcg ccatgctgcg gggcatcgtc gccatgcatc acgaccgtca
    58261 gcggttcgtt gtaccacccc agcggctgca ccactaattg ggtgtagtga cctttcgggc
    58321 tggcttccag tacctgtacc ggcagcggcg aatcgagact ggtacggcgg ctgatatcca
    58381 cttcccacgg gcgcaggaag agatccaccg gcccctgata cgcaggtgtg tagcccagcg
    58441 gccagcgatg cgcgccaaca tggaactgcc cgccgcgaat ggttccctgc aggcggttca
    58501 cttcgcccat aaattcgagt acaaaacggg tcgctggttc gcgccatacc tgattcggcg
    58561 catcagcctg ttcaatattc ccctgactca tcaccactac acgatcggct acttcggtcg
    58621 cctcttcctg gtcgtgggtc acaaacacgc tggtgaattt cagctcttca tgcagttgac
    58681 gcagccagcg acgcagctct ttacgcacct gcgcatccag cgcgccgaac ggttcatcaa
    58741 gcagcagaat ttgcggttct acagccagcg cgcgcgccag cgccacacgc tgtttctggc
    58801 cgccggaaag ctgcgccgga taacgatccg ccagatgcgc aagctggacc atttccagca
    58861 attttgtcac tttcgctttg atggctgcgg cattcgggcg ctcgcgacgc ggcagcaccg
    58921 tcaggccaaa agtgatattg tcgaataccg tcatatggcg gaacagcgcg taatgctgga
    58981 acacgaaacc gactttacga tcgcgcgcgt gcaggcgact cacgtcggtg ccgtggaagc
    59041 gaatatgccc gctggtttga tgctccagcc cggcgataat ccgcagcagc gtggttttcc
    59101 cggaaccgga cggccccagc aacgcgacca tctgaccaga aggaatatcc agtgagatat
    59161 cgttcagcac ctgggtgcga ccaaaagact tcttgatatt ggcaatctca atgctcatga
    59221 tgctcctcct gctgcgcgcg tttttcctga ttctccaggc gccactgcaa catactcttt
    59281 aaaaacaggg tgataatcgc catcaacgtc aacagcgcgg cggcggtaaa ggagccgacg
    59341 gtgttgtagt cctgctccaa caattcaatc tgtaacggca gcgaaagtgt ttcgccgcga
    59401 atcgagccgg aaaccaccga cactgcacca aactcgccaa tcgcgcgggc gttggtcagc
    59461 accacgccgt aaagcagcgc ccagcgaata ttcggcaatg tgacgcgacg gaacatctgc
    59521 cagccggacg cgccaagcaa aatcgccgct tcgtcttcct ggctgccctg gcttaacatc
    59581 accggcacca gctcgcgcac cacaaacgga cacgtcacaa agatggtgac cagcaccatt
    59641 ccgggccagg agaacataat ttgcagatta tgttcatcca gccagccgcc gagcgggccg
    59701 ttggagccgt agaacagcag atacaccaga ccggcaacca ccggcgatac agcaaacgga
    59761 atgtccagta gcgtcagcag taactggcgt cccgggaagt taaagcgcgt caccagccag
    59821 gccagcagaa tgccgaacac caggtttacc ggtacggcaa tcagcgcgat catcaccgtc
    59881 agccagatgg cgtgcagcat gtccggatcg gccagattct gtaaaaccgg catcagcccc
    59941 ttgctgaatg cctgcacgaa gatgtaaatc atcggcacca gcaggatgaa cgccgaaacc
    60001 agcatcccga tgccaatcag aaaccatttg ccccagttaa tcgggcgcgc gtcataacgc
    60061 ttcaattggg taacttccgc cattaatgac ctaccacacg ccgaccaaag cgactttgca
    60121 gagtgttaat tgagaacagc agcagcagag atgccgcgag gatcaccgaa gcaatcgcgc
    60181 tcgctgccgg gtaatcaaac tcctgtaagc gcacaaaaat catcagcgac gtcacttccg
    60241 tcttccacgc gatatttccg gcgataaaaa tcaccgcgcc aaattcacca agactacggg
    60301 taaacgacag cgccacgccc gccaccagcg ccggagaaag ctccggcagc accactttgc
    60361 agaaactctg ccagcgcgtt gcaccaagcg tttccgccgc ttcttcatat tccgggccta
    60421 actcttccag caccggctgc acggtacgca ccacaaacgg aatgctggta aaggccatcg
    60481 ccaccgcaat accaagccag gtataggtga ctttgatatc aaacttcgcc agccattcac
    60541 cgtaaaaacc gtttacggaa aagagcgagg ccagcgttaa accggcgact gccgttggca
    60601 gcgcaaaggg taaatccatc agcgcatcaa gcagcgtgcg gcccggaaag cgataacgtg
    60661 ttaaaatcca cgccatcagc agaccgaaaa cgccgttaaa aatcgatgcc acaaacgccg
    60721 acagcagcgt cactttgtag gccgcgacca cctgcgggtt ggtgatcacc tcccagtact
    60781 gcgcccagct catctcagaa agctgcatca ccagcgcaga gagcggcagc agcaatatca
    60841 ggcacacaaa cagcagactg gtgccaaggc ttaaagtaaa gcccggcagc acgcgtcttg
    60901 aagaaactgc aaacattact tacgccccgc cgctaacagc ttgtctaact cgccgccgct
    60961 ggtgaagtgg gttttcatca cttccggcca tgagccaaat ttgtcttcca cgcggaacag
    61021 ttcggtctgc gggaatttat ctttcagttt gtccatgact tccgggttat tcacacggta
    61081 gtaatagtcg gtgatgatgg tctgcgcctg tgggctgtag agccagttca ggtaggcttt
    61141 tgcggctttt tccgtaccgt tggcctgcac atttttatcg acccacgcca ccgggaattc
    61201 cgccagaata ttggttttcg ggatcaccac ttcaaagccc tgtgcttcat actgtttacg
    61261 gatgttgttc acttccgatt caaagctaat cagcacatcg cccaagccac gctcggcaaa
    61321 agtggtggtt gcgccacgac cgccggtatc gaacacttca acgtttttca ggaactgggt
    61381 cataaactgt tcggttttgg ctttatcgcc accgtcagct ttatccgctg cgccccatgc
    61441 cgccagatag gtataacgcg cgttacccga cgttttcggg ttcgggaaaa tcagcttcac
    61501 gtcggagcgc accaggtcgt tccagtcgtg gatattcttc gggttgccct tacgcaccag
    61561 gaagcccatg gtggagtaga acggcgagct attattcggc aggcgcgact gccagtcagc
    61621 cgggatcagc ttgcctttgt cgtgcaggat ttgtacgtcg gtcacctggt tataagtgac
    61681 aacgtctgct tttaagccct gcaaaatcgc cagcgcctgt tttgatgacc cggcatgaga
    61741 ttgttttatc gtcagtttgt cgccgccgtt atcttttgcc cattgctgtt caaacggcgg
    61801 attcagggcg gcaaacagct cgcgggagac gtcataagaa ctgttcagca gttccgttgc
    61861 ctgtacatgg cccgccagca gcagagaagc gaccagcgcg agtgagttct ttttcagtaa
    61921 gttaacggcc attgcgcacc cttataaatt taatgacttt ctaatagcca tcatatttat
    61981 aacggacgtg aaaggagtaa cggttttata taccgtttgg tgatttggaa gttaaaaagg
    62041 gaataagaac ttggttcaac aggcgtaatt gttgcaggca gtttggacac ggacagcgcg
    62101 gagaaaccgg agcgtacaca tagtacgtga ggatttcgag cactgcccgg ggccaaaatg
    62161 acaaatgaaa tagcctggtg aacttagttc aagacgaaat cctccccaca catgcaggga
    62221 ggaaacagag gtgaatcaga taccgacgct aaccgtctcc ggcagtgtgc tgccgccatc
    62281 aatcacattc tgtgtaccgg ttaaatagct ggattcatcc gatgcgagga aggccgccag
    62341 ttcgccgact tccagcggat cggcgaggcg acacagcggg attgcttttg ccatttcagt
    62401 cagcaccgat tctggatctt ccgggttcga ctggcgggca atgctttccg ccattggcgt
    62461 gcggacgtat cccgggcaga tggcgttaac gcgaataccg gattgcgcgt actccaccgc
    62521 cagcgattta gtcaggccaa caatcgccgc tttcgttaag gcatacgccg tttcgccagg
    62581 atcggccacc atatcaccag tgactgaaga catcatcaca atgcgaccat ctttgcgcgc
    62641 aatcatctcc ggcagcaccg ccttcgtgac gttccatacg cctttaatat tgatatcaat
    62701 atggaaatcg cgatcttcat cgctcatatc gaggaaactg cccagacgac aaacgcctgc
    62761 gttattcacc aggatatcaa tgcgcccttc tttttccttc gcgcgtttga tagctgcggc
    62821 taccgacgcc gggtcacgca catcggcgac aaccgccgta cagcgatgac cacgaccaca
    62881 cagttcgtcc gctagctttt cgatctcagg ggagatatcc agcaagatta ggttcgcgcc
    62941 atgacgtgca aaagttctgg caattccttc gccaattccc tgcaatgcgc ccgtaatcag
    63001 tgctgtcttg cccgtgagtt tacccatttt aatgctctcc ttgttaatac agagcgttac
    63061 actccattcc ctctgaaaat acaggataaa caaaatattg ctgcggatca gctcactaat
    63121 tcaggaaaat ctcaatccag tcgctgcttt atgcatcatt tcaggcgttg tgttagctca
    63181 ctgctgcgct gaatcatttt caatgacgcc acatcattga tgttgcacaa gaccaacaaa
    63241 cagtaaatcg gtaacagaat tttgcgccgt gcgggtagac atcgacgagc tacgccattc
    63301 ggtttcaccg gaaacggtat cgagcgtgaa atgcgccagc cgccgcagcg gagagtcagc
    63361 cagagaagta atggcaatca ccgttgcccc ctgctttcgt gccgcttctg cgcacaagac
    63421 aatctctttt ttactgccgc tgtaagagat agcgatttgt acatcgcctt ttttcaacgc
    63481 ctgggaaacg gtggcttgta cgtgcgtgtc ggcttcgcag gccacgcgat agccaatttt
    63541 catcagtttg aacgacagat cgcgccccac cagcgccgat ccgcccaggc ccgttatctg
    63601 gataaacggt gctttactga tgacctcaat gattttttgc agccgcgcgt agtcgaacaa
    63661 cgcacaggtt tgctccagcg ccagctcttt ttcgcgattc agtttgcggg caatcacttc
    63721 cagcgaatca tcactggtaa tcgaactgtg caggtgcagc gccgtggcat tcgttttctc
    63781 ccggctggca ctgtattcgc caattaacgc catacgcaat tcagtaaatc cttgcgcgcc
    63841 gagcttctgg gcaaatttca caatgctcga ctggctgata ccgagctgtt tcgccatctg
    63901 ccgtgaagag acggatttca gctcgctcac tctggcccgc agaaagtcag caattttttg
    63961 ctcgttcccg gtaaattcac tttccgcatt gcgtattttc gtcaggtaca acatcttcca
    64021 ctctcctggg ctggcagcaa aaattccgct taagttctta tgacgctctt cacactctgc
    64081 gagtgtagac tcaatgattc ctttaagtct gtcaattcgg gatatatgat tccatacatc
    64141 tgcaattatt tgatcgtaaa tagtaaggtc accaccgatg caactagaaa agatgattac
    64201 tgaaggctcg aacaccgcct cggctgaaat tgaccgcgta tcgacgctgg aaatgtgccg
    64261 gattatcaac gatgaagata aaaccgtacc gcctgccgtt gatcgcgtcc tgccggatat
    64321 cgccgcggcg atcgatgtta tccacgccca ggtcagcggc ggcggtcgtc tgatttatct
    64381 cggtgcggga acatccggtc gtctggggat tctggatgcc agcgaatgtc cgcccaccta
    64441 cggcgtgaaa ccgggtctgg tggttggttt gattgctgga ggcgaatatg ccattcagca
    64501 cgcggtggaa ggagctgaag atagccggga aggtggtgtt aacgatctga aaaatattaa
    64561 tttaacggca caggatgtgg tggttggcat tgctgccagc ggtcgcacgc cgtatgtgat
    64621 tgccggactg gaatacgcac gccagctcgg ctgccgcaca gtgggaattt cctgtaatcc
    64681 ggggagcgcc gtttcaacca ccgctgagtt tgccattaca ccgattgtag gtgccgaagt
    64741 tgttaccggt tcttcgcgga tgaaagcagg tacagcgcag aaactggtgc tcaatatgct
    64801 ttccaccggg ctgatgatta aatccggcaa agtgttcggc aacctgatgg tcgatgtggt
    64861 cgccaccaac gaaaaactgc atgtgcgcca ggtcaatatc gttaaaaacg ccaccggatg
    64921 taatgccgaa caagcggaag cggcgttaat tgcctgcgag cgcaactgta aaacggccat
    64981 tgtgatggtg ctgaaaaatc tcgatgccgc agaagctaaa aaacgcctgg atcaacacgg
    65041 cggctttatt cgtcaggttt tagacaagga ataacccatg gccaaagaga tcagcagtga
    65101 acttctgaac accattctta cccgtgtcgg cggaccggga aatatcgcca gttgtggtaa
    65161 ctgtatgacg cgcctgcgtc tgggtgtaca tgacagttca ctggttgatc ccaatatcaa
    65221 aacgctggaa ggcgtgaagg gcgtcatttt gaccagcgac caggtgcagg tcgtttttgg
    65281 gcctggtaaa gcgcatcgtg ccgcaaaagc gatgagcgag ctgctgggcg atgcaccggt
    65341 acaggatgcc gcagaaatcg ccgcccagaa caaacgtcag ttaaaagcca aacaaacctc
    65401 cggcgtgcaa caatttctcg ccaaattcgc caccatcttc acgccgctga tccccggttt
    65461 tattgccgcc ggtctgctgc tggggatagc gacgttaatt gccacggtga tgcacgttcc
    65521 ggcagatgct cagggcacgc tacccgacgc gctgaatttt atgaaggtgt tcagcaaagg
    65581 tttgttcacc ttcctagtga ttctggtggg ctataacgcc gcccaggcat ttggcggcac
    65641 gggcgtcaat ggcgcaatta ttgccgcact ctttttgctc ggttataacc ccgctgcaac
    65701 caccggttac tacgccggtt ttcacgattt ctttggtctg cccatcgatc cgcgcggcaa
    65761 tattatcggc gtgctgattg ccgcgtgggc ctgcgcccgc attgaaggca tggtgcgccg
    65821 ctttatgccg gacgatctcg acatgctgct gacctcgtta atcaccctgc tgatcaccgc
    65881 tacgcttgcg tacctgatca ttatgccgct gggtggctgg ttattcgaag gtatgtcatg
    65941 gctgtttatg cacctgaaca gtaatccgtt cggttgtgcg gttttagccg ggctgttcct
    66001 gatcgccgtg gtatttggcg tgcatcaggg ctttattcct gtttacctcg cgttaatgga
    66061 cagccaggga ttcaacagct tatttcccat cctttcaatg gcaggcgcgg gccaggtggg
    66121 cgcggcgctg gcactctact ggcgggcgca accgcacagt gcgctacgca gtcaggtacg
    66181 cggggcgatt attcccggcc tgctgggcgt tggtgaaccg ctgatttacg gtgtcaccct
    66241 gccccgcatg aagccgtttg ttaccgcctg tttaggcggc gcggcgggcg gtttgtttat
    66301 cggattgata gcctggtggg gtctgccgat gggcttaaac agcgcctttg ggccgtctgg
    66361 tctggtggcg ctgccgctga tgacttccgc acaaggcatc cttccggcaa tggcggttta
    66421 tgctggcggg attctggtgg catgggtttg cgggtttatt ttcaccacgc tctttggctg
    66481 ccgtaacgtc aatctggact gatattatga aacggacaat gctctatctt tctctgctgg
    66541 ctgttagttg tagcgtcagc gccgcaaaat accctgttct gacagaaggt agttgatcaa
    66601 gtatatgcgg cgttaaagca gtaataaaaa ggccagtcag taatgttact gcctggcctt
    66661 taatcaatga atcagaaacg cttacagcgc catcaacttg tccagcgacg gtgcgaaata
    66721 atagccgccg gttaccggtt tggtgaaacg caacatcgca tcacgtttgc catcggtatc
    66781 gccaaacatg ctcagcagtt gctgctcaat gttatgcaga cgcgcgcagt aggcgcagaa
    66841 gtacagaccg tgagtgccac tggcggtgcc gtacggcagg ctctggcgaa caatcttcag
    66901 ccctttgcca tcttctttca gatcaacgcg ggtgaggtga gaggtttccg gacgttcgtc
    66961 gccgtcaatc tcttcgttgg cctctttggt gcgcccgatc atcatctcct gatcgtgaac
    67021 gctcatccgg ttgagctgct tcaggttgtg ctcccaacgc tgcacaaaca catagctgcc
    67081 gccggaatcc acgccgtctt tgataaccgc cacttcgcgg cgcgtctctt cacccgccgg
    67141 gttttctgta ccgtcaacaa agccgctcag gtcacgctct tccacccaac ggaagccgtg
    67201 gatctcttct ttcacttcaa tgcagtcacc aaaggcttcc atcgccgcct gagcgacaga
    67261 gaagtttacg tcgtgacgca gagaaagaat gtggatcaac acatcgaact gggtcgtcgg
    67321 cgcaaggcct ttaccgtagc ccggaaaatc tttcagctct tctgccccaa cgccgccgct
    67381 cagagcgcgc caggtgttgt taccaaaggc aaccaccgca ccaagatgcg cgtccgggaa
    67441 tttcgcttca aaagttgcca gtttgtcggc aaatgtttta ctggccgcac gcagggcgtc
    67501 aacttcccct ttcacgttgg cttcgatcca aatcgccgcg cggcaatgtt ctggcaaaat
    67561 gccactctga acctgagaca ttgttcctcc tgaaaataag aatgccacgc aatccgtggc
    67621 attgttgagc gctattttac gtgttttttc gcgacggcgg tttgttcaga cgcaaattaa
    67681 cgccgccaga taattttact gactttccag tttttcaggg tatcgtccga aggcattaac
    67741 ccttcaggac cacgccattc cccgctgaac tggtagctaa tatgttgact gccttcggct
    67801 ttacattcga cggcacgatt atcatcacca tcagcttttt ggcaattgcc aaatgctttg
    67861 ctgtaaaggt cgctaaacgg tgtaccgatt ttaacaccgg tgtcagcagg aatatcgcta
    67921 tccagcacgt caatgcgact gatcgtgcct tgatcgccgt taatcaccat cgccacgttg
    67981 tcgcctttca tcacttcaaa aaagcgcacc acgttgccgt tcgcggtttt cattccgctg
    68041 cgcaggcgat aatcgccatc aagcgcatca gcaatggctt gttcttgcag tggtgtggac
    68101 gccgttaatt cacccacgcc ctgctcgctc actttggtgg acgatccaaa ccagttccac
    68161 gggttagcgg cagaccagtt aactgacgac atcgtggaac agccggtcag catcaacggc
    68221 atagcgcata acattaaacg cagcgatttc atgtcacttc ctttggttat tcaataacgt
    68281 tgcttggagt gcaaattcac caaaaagtgc cgttattctt tttcttcatt aaaacaagcg
    68341 cgtaaacgtc gattggtcag cagccagatt aacgccacaa tatccgccac taccagcgcc
    68401 agaccgatac cgctaacgga ttcaccgttc agccacagcc acggttgcca gcaaagtaaa
    68461 accacctgcg ccaacagcaa cagaaaatag agcacatgcc aggtgcgagg aaacgaagcc
    68521 cgccgaccgc tcaacaaaaa cgccagcacc gccggaatac cgggaatcag ccccagccag
    68581 aaattatcgt gatcgggata aaacagattt agcagcgcag taccctgctc acgcgacgca
    68641 ccggcaatga caaacagcac ccaggttcgc gcctgaagca atagcacaag ccagaagagc
    68701 aagggtaaac gcaggcgtcc gtgcgcatca taatggacag gatggaactc agtactcttc
    68761 atcttcaatc aaacgcttac ccagactcag cacgtcggcg tgttcatatc ccaggcgttc
    68821 atacattccg agcaccatgt cgttatcttc cggcacgttg atctgaattt tcgggcagcc
    68881 gcgagcaatc agctttttct ccagccggtt aagcaacgca ttggcaattc cacgcccacg
    68941 aaactctgga tgcacgccaa gataataagc agacccgcga tgcccgtcat aaccgcccat
    69001 caccgttccg accacttcac cgtttacctc agcgaccaaa aacaaactga cgtcatggtt
    69061 catcttacgc tcgatgtcca tttccggatc gttccacgga cgcagcaagt cgcaacgctc
    69121 ccaaagggtg atgacctctt cgaaatcttc ctggcgaaat acgcgtatct ccatggtatt
    69181 cgttaccttt ttgcgggtta aaaggctgat tatggcgtga acggtcgaat tagccaatat
    69241 ctgacgaaaa tcggttgaaa aagtggcata atggggagtt gtcaactatt gaaatgaaaa
    69301 gtaaaacaat tctcaacagc aaaccgtcgt aacggattac gcgatacgat ataacatctg
    69361 gaactttatt attacaactc aggccgtatg agcactttta aaccactaaa aacactcact
    69421 tcgcgccgcc aggtgctgaa agccggttta gctgccctga cgttgtcagg aatgtcgcaa
    69481 gccattgcca aagacgaacc tttaaaaacc agcaacggac acagcaagcc gaaagccaaa
    69541 aaatctggcg gcaaacgtgt cgttgttctc gatccaggcc acggcggaat tgataccgga
    69601 gcgatcggac gcaacggttc gaaagaaaaa catgtggtgc tggcgattgc taaaaacgtc
    69661 cgttccattt tgcgtaatca tgggattgat gcgcgtttaa cgcgttctgg cgatacgttt
    69721 atcccacttt acgatcgcgt tgaaatcgcc cataaacatg gcgcagatct gtttatgtca
    69781 attcatgccg atggctttac caacccgaaa gctgccggtg cttcggtatt tgccctctct
    69841 aaccgtgggg caagtagcgc aatggcgaaa tacctgtctg aacgcgaaaa ccgcgccgat
    69901 gaagttgccg gtaaaaaggc gactgacaag gatcacctat tgcagcaagt actgtttgat
    69961 ctggtgcaaa ccgatactat taaaaacagc ctgacgcttg gctcgcatat tctgaaaaag
    70021 attaagccgg tgcataaact gcacagccgc aacaccgaac aagccgcgtt tgtggtgttg
    70081 aaatcgccgt cggttccttc ggtgctggtg gaaacctcgt ttatcaccaa cccggaagaa
    70141 gagcggctgt taggcacggc ggcgtttcgt cagaaaatcg ccacagcgat tgctgaaggc
    70201 gtgatcagtt atttccactg gttcgacaac cagaaagcac attcgaaaaa gcgataagtt
    70261 atgaaacccg acgcacacca ggttaaacag tttctgctca accttcagga tacgatttgt
    70321 cagcagctga ccgccgtcga tggcgcagaa tttgtcgaag atagttggca gcgcgaagct
    70381 ggcggcggcg ggcgcagtcg ggtgttgcgt aatggtggtg ttttcgaaca ggcgggcgtc
    70441 aacttttctc acgtccacgg tgaggcgatg cctgcttccg ccaccgctca tcgcccggaa
    70501 cttgccgggc gcagtttcga ggcgatgggc gtttcactgg tagtgcatcc gcataacccg
    70561 tatgttccca ccagccacgc gaatgtgcgt ttttttattg ccgaaaaacc gggtgcagag
    70621 cccgtttggt ggtttggcgg cggcttcgat ttaacccctt tctatggttt tgaagaagac
    70681 gccattcact ggcaccgcac cgcccgtgac ctgtgccagc catttggtga agacgtttat
    70741 ccccgttaca aaaagtggtg cgacgattac ttctacctca aacatcgcaa cgaacagcgc
    70801 ggcattggcg ggctgttctt tgatgatctg aacacgccag atttcgacca ctgttttgcc
    70861 tttatgcagg cggtaggcaa aggctacacc gacgcttatt taccaattgt agagcgacgt
    70921 aaagcgatgg cctacggcga gcgcgagcgc aattttcagc tctaccgtcg cggtcgttat
    70981 gtcgagttca atctggtctg ggatcgcggc acgctgtttg gcctgcaaac tggcgggcgc
    71041 accgagtcga tcctgatgtc aatgccgcca ctggtacgct gggagtatga ttatcagcca
    71101 aaagatggca gcccagaagc ggcgttaagt gagtttatta aggtcaggga ttgggtgtaa
    71161 ctccctcacc cccattcccg catccgctga tgcaacgtca acgactgctt ctcggcaaac
    71221 agctgctggt aatccgtggc aaattgcccc agatgccaga atccccactg catggcggcg
    71281 tcttttaccg ttgtgctttg cgaccacggg cttatcagtt cgcggcgtac ggcgttcagg
    71341 cgaatgcgtt tcagccacgc gtttgggcca atgcctaaaa tagcgtgaaa cgcgttttgt
    71401 agcgtgcggc gactgacatg cagttgatta cacaaatcca gcaccgtcac cggttcggac
    71461 atgttttcca gcacatattc acgggcgcgg gaaagcaacc gacggtaact ctgatgactg
    71521 atgctttccg ccgtcaccat tggttgcgct tcttccagca tcgcccccat cgccattagc
    71581 aaattatccc ccagcacttt tcgcactgca ggctgatgga gattttccgg gttctcgcaa
    71641 aacgtcgcca gcgcctgttg gacaaagccc cacagcgcgg ctttatgctg ctctttcact
    71701 tccagcgccg actggctacg cagcatatgt aatacccgat ccggattgtg caaaaagtta
    71761 gcctgacagg tgatgacatc ttcagacagc actacgccca ggatcgtata atcgtccggc
    71821 gtacttaatt caaactcggt tccaccgggg cgcgtcgcta tttctgcgct tcccagacat
    71881 tgcgaaccga taaatccctg ctcaccgcgc gtcgccggaa tgccaaacca gaacgagttc
    71941 ggccagacca ggcacgactg acgcagcgcc agaccggtgt attcacgaaa aacctgaata
    72001 tcatcgagta gaatttccgt aaattcacca tgaaacttgc ccggatgcag ctgatcgtaa
    72061 atctgctgct aggcggtaat cgtcaaagca tgttcataga catccgttgt ctgtcgttga
    72121 tgaacattat ccacttcgac cttcggcgtg agcttcaggt tttcgggtaa gggttcatga
    72181 taaagatggt gcaaattggc tgtacgggtc tttttcatga tgttaatgcc gggtgttgta
    72241 ggacacccgg cacctccgac aggttaatgg ggcttgagac gataacgact actgcgttta
    72301 cgtaacgtcc cggcagaaaa gagctgttcg agcgcatttc tggctttttc cagcggccag
    72361 ccaaagtggg cggccacttc tcctgccgtc attccctgac atactgatgt taacagcgcc
    72421 aacaactcgt cagcagattc cgcgacgata actggcgcgt cgggttcctt tacgggttgc
    72481 ttcggctggc ggttaaagcc agtaaccagc cactgggtgt catcgtctgg ccgaccgatc
    72541 tccttacggc tgatgacacg cccggtacgc atcgcggcag cacaacctgc gtccagcgcc
    72601 gcacgacacg ccgccagatc gccttccacc accagcgtta agcgaccagg gtcaagcact
    72661 tcgtgactga gcagacgaac gttagctgct ttgagcatgg catccgctgc atctatcgcg
    72721 gcgaccatgc cgtccacttc cagcaatccc agtgcattga tcatcggcaa cctccgttac
    72781 gcacgctgga ttggattacg cgcgatttcc agcactgcat cggtaaaggc gttacaggct
    72841 gctttacacg cggcctggct accggttaaa aatgctgccg agtagttggt ttcagacggt
    72901 ggcgggacgt aggtcaccag ctgtacgtcg gcagatttca acgcggcatc aatgccgtag
    72961 gtcgcttcca gcggcggtgc caccagatac gccatcggat cgccaagcgt gatcccggcg
    73021 gttgatgaaa gataagaacc ggtacgcgaa accacatgcg ccaggaaggc ggtgttttct
    73081 gcgtcattgg cccactggaa agctgcgcca ttttcaatat gtgcaaccat cgcatccaga
    73141 ccggcacgca cttccgccgg gtttggccca ccaagcataa tcagcacttc accggcagtc
    73201 ggtgacgggc cgtgtgccgc tcccgcatac agcgagcgac catataccac ttcgaccatc
    73261 gcttgtttgg tcgcttcgtc agccgcaatg taggttacgt cgtcagaatc ggcagaaatc
    73321 agcccgaggc tacgaatatg cggcggcaat tttagttcac gcgcaaattc agcgttaacg
    73381 gaggcaatca cccgcatggc ggttaccgac ggtcgaatca aatctaaagc tggcatgatg
    73441 cctccttatc gggtcatgtt gatgccggat gctttctgct ccagcatacg tttcgccaaa
    73501 tccacaatga cggcacagca gcacgaaaga gaagcaaaag tcagctacct ggtacactag
    73561 gcagcgggtc agcaaagggg ggcaaatgcc ccttttttca tgtcagataa tcagatcatt
    73621 acctcaataa actaataatc acatcgcggt tacaaccagt ttgatagtga tagctcaaac
    73681 ccagacgtgg cgctggctgc aagcaaaata agccgcaaaa atgcgacccc atgtttacta
    73741 gccgcaaaaa tgcggtccta tttgccagaa ccttttatta ttcaattggt tagtgcaaga
    73801 aaaagcgtaa ttactgttca agaagtatcc agaaaggtca tgattttgac gctgaaaaaa
    73861 aataggtggg catttttgcc cactcactag ccgcaaattt gcccactcac tagccgcatt
    73921 tttgcggccc aaagtaataa atacagagaa ataataaata cagatcagcg ccaagatttt
    73981 tcgtcttttc ggcggaatca tgagactagt gcttacgtcc tgataacctc cagagcagac
    74041 cttaacttct gtgcagcagg actacgctca ttgccgctcg gctaggtcaa atcctcacga
    74101 taaagctgtt cggattgtga ccataatgtt aaaggactct ttaaatcatt ccttgacgaa
    74161 ttaactagct ggcttaagcg gaatagcatg caggataaaa acaaaaccag cttagcagtg
    74221 gggagagctt cgcttcgctc gtctcctggg gccaatgctc aatgaaacga cgtaaggcac
    74281 ggagcgctca ccataggtag acacataaac atctgagagc gcaccttcta tcgcgctacg
    74341 cgctcaataa aaaccaacga caccgccaca ctgcaagacc aatggttttt atcgcgtata
    74401 cagaccagac aggaaacagc agcaaagcag cgtatagcca ggtgcagaca gcacactcac
    74461 acgacgcaca gcaggcttaa gtgatcactc aggaaacttt atccccgccg ccgatcaccg
    74521 cgaactatca tgaccgcgcc aaattcgaaa ggaatggccc atagaacgcg atgaacacac
    74581 ataagcgcta cacttactta acgtcgccac agagcgattg agagcgctta aaatcgatgc
    74641 gatttcatta aatttttctc actcgcctga atggtggcga tcaggactga tcgcaacatt
    74701 gccccgcctc gatattgagc gcataataga tacatacatt aagcggaaaa gagataagtt
    74761 tattgtgaaa atcagggggt tacatatcat gcgcatatca gaacaacaac ggcagatcct
    74821 cgactatctg gcaaaaaacg aatcaatcac cgcacgacaa gccgcagatc tcgtttatgg
    74881 tggcaatgtt acccgcgtcc aggttgacgc cgcccgccgc tcactcatga cgatggttag
    74941 taatgggcta atcaggaaac aaggccgcgc tttcgtagct gaaagacata tcaatcacgc
    75001 ttatagccag gacgttgaag aattgcgccg ccagatcctt tgggtattca gcaaacaggg
    75061 tcaggagtat ttcctttctg aacacccaaa tttcgccgcc aaagagttaa cagtaagcgg
    75121 attgtcgtgc gcactggatt atcttgtcgg tgaaggttgc gttacttaca agcagctatg
    75181 gcacgccctg aatacgcttg tagaagatgg tttactcatt gccacgcgtg agaacatcga
    75241 atactggata atgccgcgtt caggtcgccg caatcatgca cggtatattt caggcaacag
    75301 atacagaacg ccggacttta atccgacaca ggaagatcgg gaccgtgccg aaatgatgtg
    75361 cagggaacag gagaaaagaa ttatggataa gttattttcg atgcagtgaa cacaagcggg
    75421 cgcaatgccc gtttttattg ccttattcca cgcctgacga atcaatttga tcacacctga
    75481 gaaaatgcat agccagaagt taaggccgtt atattgcgat tgggagcgtt ttaaacggta
    75541 tcaatttaat taattctgat gtggtgattt gcaggcaaaa aaaacccgcc ggagaagcgg
    75601 gaaatagaga catatcgttg tagttattaa cagatcactt tgaggtcgag ggcggctaac
    75661 cctcgctaat attatcccgc tattaccttg cgggtgcaaa taatctttta ttgctttgat
    75721 gatttaataa atagcacttt ttgctaacaa aaactgctgt caatatatgg ccaaaaatgt
    75781 caacagaaaa aataaggttt ataatattaa gtatcacatg ggtaatgggt tcagggaatc
    75841 ggaatccacc gcctcacgga aattcaaaat ttgcttcttc tttcctccgt gggcggcttc
    75901 tttttcgtat cgaggtattt atgaaagcaa taacattaga acagttcgca gcaatggcga
    75961 atgtatcgac cagaacgctg aggcgctggc taaaaaaacc atcaaccgga gtccctttgc
    76021 catttacccc acgcggtact cgccaattat tctgggagca cgaatgcaaa gaatggttgt
    76081 acagaacaga acgataataa tcacgcatca cattttgagg taaacatcat gaccaatatc
    76141 gaaagattag aacgctgtgc cgatattatg cggcgcagat ggatttatga ccctgaagcg
    76201 ggtttattgt ttagccgtga aacaaaagac gttatcaaag gcagcatgac caataaaggg
    76261 catttggttg taacggtaca tgaaggtaaa ttcaaagtga cactcaccta tcagaaagcc
    76321 tgctatgtat acacatacgg cgcttatgac gagacattat atgaagtgca ccacgttaat
    76381 ttgaacaaac aggataaccg catcaagaat attcgcctga tgccgaaaga aaagcaccgc
    76441 cggatacata aaaacgtccg caaacttatt cggctgggcc acacacaatg gcttcacccg
    76501 caattaattg aacaatcact ataaacacag gagtaaatac catgcattca ttagacagtt
    76561 attttcagcg cacgacagcc cctaaatcag ccgcccagga acgccgtgag gaattccatg
    76621 agaaagtgat gcgttctgct gattatattg cggacaagtt cgtggaaacg gttcgtccac
    76681 ttgttgatga agtagctgat aaacttcagt ctgaaatgcc ggaagatatg gaaggcactg
    76741 caaagcggcg actcatttgc gaattaagtc gtcgttttgg cgtgtcaata agcgctttta
    76801 aataatcgaa aaaagggggg aaagataatg gacgcatttg aggtactggc aaataaaggc
    76861 gctgaactgg tagcacagcg tgataaaacc gccaacgaag gggaacgcgc actgctaaat
    76921 aaacagatta aagcgatccg tatggcccag tttaaattaa tcagtaatga agttatcgaa
    76981 acaattaaac cgcagatccc acaaatcgtt gctgacgcta ttaaagccgc cggactggag
    77041 aaacgcattg ccgggctaaa aacgaacgca gagaaaatgg agatgcacaa acagagcggt
    77101 gctccgaatc ccgatgagta cttctcaccg gatgttgagt ttatggcgca ggttgaggaa
    77161 cgtctgggta gctgtctcac cgaggaacag cgtcgctatt ttgatggagt ggatagttcg
    77221 gcaggtattg acctgaactc gtatttcggt cgtgaaattg agcattttga tgccgccccg
    77281 tctatgccgg agccagaacc agaaatggcc accgatcctg aagctgagca acggaaacgc
    77341 gtattcaatg cgatagggat tcggtactga tcggataaaa gaaagccggg aattaccccg
    77401 gctgacttaa caatgtgacc aatgaacaaa tcggacaaga caaacaggat aagcagtctc
    77461 gaaacaatgt tcgggaacta tattagcaca agtccggaga aaatcatcat gttggcacta
    77521 cgcatcatgc agggcatcgc taagaccctc gcagaacatg tcttagacct gaaacattcg
    77581 ccactgagca aacaggccat gaagcgccaa acgcttcgtc tgtgggctga atattcgctt
    77641 ggaactatca ataaaatcat cgacatgaaa tcggggccga gcaaccagag cgcggaggaa
    77701 atggagttca tcaggcgttt aattctcatt cgcagagaca ttcatagcca gttacacagc
    77761 gtgggaatcg atattaatga tgggacgggc gattaatttt cggacaagta ctatgcgcgc
    77821 gcgaggagct gaacatgaag caagatattg cagacaggct ggagatactc gaaggccagc
    77881 gggcagaagc gaaacaacta cgtaagcagg ccagaagagc gcacaggaac aacgaagcag
    77941 agttactaac caaatacatc agtttcacca attactgcat ctacgagtgt tacaaggaag
    78001 atgcagagga ctggctggat tcattgccag aacagtactg agcagaataa caccacgtca
    78061 tgttgttctg aggtgagcat atcacctctg ttcaggtggc caaatttagt gtgccacttc
    78121 agcatcaccg ttgaaatcac aataacgatt tttttgtaaa tatgctctca catatgtagg
    78181 aatagaccag gttggcattg aaacacatct accatgacca taataaagta attttttgat
    78241 atgtttatgg ttttatatta attaaaatat atctaaaatt gatagtttta aaattgcaaa
    78301 atccagattg tgtttgtaat gtttaatctg attgtattga gtggcgttta ttatgaaaaa
    78361 taaatcaggt aattcttgtt atagagaagc agtcgatatt catagctcac ttgagctttc
    78421 atttatattg cataaatatc atttgcgtgt agatgagaag aaatctcgaa atgtattcag
    78481 cgccatacgt gatgagctca tccttgatgg ggattcacga caaaacttag ctactttctg
    78541 tcaaacatgg cttgatgatg aaatccgtga gttgcttaat ttagctattg ataaaaacat
    78601 gatttataaa gatgaatatc cacaaatggc ggaacctgaa ggtcgctgtg tgcgtatgct
    78661 ttccgattcg tggagtttcc ccgattcgcg acacacctta ggttgctcaa ccattggttc
    78721 atctgaagct gctatgctcg gccgcttggc gttaaaatgg cagtggtgta aaaaacgcga
    78781 agtacaaggt aaatcaaccg aacctgattt gcgatcctgt gcaaatatgc tggcataaat
    78841 tctgccgtta ctgtgatgtg aaattgtgtg aaattccact tgatgatcat cacctgataa
    78901 tgaatgctaa agaggttttg aagcatgtcg atgaaaacac tattggtgtc gttacgactt
    78961 taggtattac ttatttttgt aaagcagatt gcgttagtgt ttttaagata agtgttaaag
    79021 ttaatattag tttttatatt tacatcgtaa ttatatgttt ctattttaaa aatgttcgag
    79081 agtataaaaa tgtctaatgc taatatcgca cccaaaagta agaaaatgac agtggggaca
    79141 ctcgctatca tgaatataac tgctgtagta agtctacgat ttataccttc tgaagcagaa
    79201 tacggcttag gtgccatttt ttactatacc tttgctgcaa ttatgttttt agtacctgtt
    79261 gcctttgttg cggctgagct agctacaaca tatccacaaa aagggggggc atttcgatgg
    79321 ataggagaag catttggggc tcgctggggt tttgtaggaa tgcttatgac ctggctacaa
    79381 gtcatcccct actttccaac agtactgaca tttggtgcag tctcggttgc atttattgat
    79441 gttgatatgg gggtggcaga atcaattgcc agtaataaat ggtatatttt atttttcgta
    79501 ctatttgttt actggggagc tatttgtgtt gcattacgtg gcgttggtat tttctcgcag
    79561 gtatctaagt ggtgcggtat tatagggaca attattccag ctattatcct tgttattctt
    79621 ggtttctcct atctgtggtt cagcggtaaa ccagcggtga tcccattagg ttggggtgat
    79681 cttttcccta atatagctaa cttccagaat attgttcttg ccgccagcat atttcttgct
    79741 tatgcaggga tggaaatgaa tgcggtccat gttaacgatt tagataatcc aactaaaaaa
    79801 tacccaattg caattaccat cgcttctata ggtactattg caatattcct gctcagtact
    79861 ttaggggtcg cctttattat tcctacagat aaaattagtc taactcaaag tttgttggtt
    79921 gcctacgata tgctgttcga atgggctgga gtgccatggc ttggctctgt gatggcattt
    79981 atgctagcaa ttggcgtact gggtggggtg gttacttgga ttgcagggcc taatacaggt
    80041 gtattagcga tcgcgaaagc tggttattta cctaagtttt tccaaaaaac caatcgtcat
    80101 ggaatggggc accatctaat gtttgtacaa ggtataattg tctcagttct ttctgttact
    80161 tttgtgataa tgccttcagt tcaagcagct tttcagattt tatcacagtt aacagttatg
    80221 ctctatttag ttatgtatat gctcatgttc gctagtgcta tctatctacg acattctcaa
    80281 cctgatacaa aacgcccata tcgagcccct gctttatttt tctgggcaac acttggattc
    80341 ttagggtctt ttctggcttt tgtattaagt tttattccgc ccgagcaaat cccaactggt
    80401 agtccaacca gctatgttct ttatttgtta gtattagttg ccctattttt attaattcca
    80461 atattaattt acgccttccg aaaatctgac tggaaagatc caaactctga tttcgaacca
    80521 ttttcttggg aaaaaagaac tagtaattaa attttccata agctacaaaa gatgaggtta
    80581 aatatgttta gtgctgaatc tccaattggt gtaatagact caggtgtagg tggattgaca
    80641 tcggtaaagg aaataattaa tttgcttcct ggtgaagata ttatctattg tggagataat
    80701 atgaatgcac cttatggcaa ccgttctgcc gatgatatca tcagtttgac taaaaaaatg
    80761 ctgacttttt tacagtcaag gaatgtcaaa ctagttgctg tcgcatgtaa tactatatcc
    80821 tcaactttgg aatccgaaga atacagcgga tatgctaaga gctttccgtt tcctatctta
    80881 tccatcattg aacctgcagt agaagatgtg attcgccagc aatataaaaa tgttggtatt
    80941 atcgctactg agtttactat taaaacgggg tgtcacaaag agctaataaa aaaattgaat
    81001 tcaacaataa atgtatttgg agaaccaagc aaaaatcttg caatgttaat tgaggaaggt
    81061 aatcttaatg ctcctgcaat cttgaatgac atcaaaaaac atgttaacca tttaatttcc
    81121 ctccatccag taaatgaaat tattctcggc tgtactcact atccaattgt tcagaatttt
    81181 tttgaagcag ttgcaccgga tataaaattt attaatcctg cacatgatca ggctatctct
    81241 attaaaaatc acttgggaca gctaaattta ttaaactcct caaatatagg aactttgcaa
    81301 ataaatacta gtggaagtat ggagatctac aaaacggtac taggggaatt gagcattacg
    81361 aaacctcata cattcagtat caggcagttt taaatttatc cggtgcaccg taaaatcccg
    81421 tccttctgaa ctgcacccca aaagttggac accaactgag taaggtgcat tttatggaca
    81481 aacccagtta catactgggt agtgcatcca attagtagaa catgtgtttt tcgataaacg
    81541 ctccgatcac tttttcgtgg atctcaactg agcgtgagaa gcagattgtt ttacgaccca
    81601 accgcttaat gcgggtgcgt agcgtcagat tattacgctc aatgcgttgg gtgaatattt
    81661 tgccggtcag atgcttattc ttcggcacct cccggccata gctgccccag tcatcgctgg
    81721 tcagcatgcc gatgttgaag ggtgtaagca gtgccagtag ctcccggcac gtttgatcgg
    81781 ttcggggacc aaaagtgtag gccagtacac cgcctgtttt ggtgttgtac gcgtacccga
    81841 gccaatgttg ccgggcttta ctgccaacgt agctccattg ctcatcaagc tcgcagataa
    81901 acgccacatc agcatgggca acaggcgaag aagttattcg ctttggcgcg agttttttaa
    81961 agtccggatg acggtgttac taccaatttt cagtgtcctg gcggtatcgc gaaccccggc
    82021 accattaaag gccatttcag ttatcagctc tttcataccc ggcttacgtg cttgataagt
    82081 ataagtgaac tgaaacacac ggtggcagtc acggcagcga agtctgtcac ggcctttagg
    82141 gttctgacca tggcggtaaa cctgagctga ctgacaacgg ggacaatgaa tattaacgct
    82201 ggtcatgaga taacctcaaa agcccgtatt atacatcaga ttcaactaat tagaggcatc
    82261 acccatccag cattataaaa catactaaca ataactaaat tataataatc acaacttaca
    82321 atcaaataat aaatgcataa gattaacaat gattttttcg ggttatattt atctaaaaaa
    82381 gcagaatatt atgtaactta ttattttcta agcgaaaatt tattgtgcgg tcagactaac
    82441 aaacttgcgt accacccgaa gaacatgcga agatttaagt attctttctt ttttggtttt
    82501 tatttttcat tttctctttt cttctctttc cggttctgtt ctggttttgg ttttttcctt
    82561 tttattttta ttctttcttc agggttatgc taaaacacca aaacattttt caccacaaaa
    82621 gcatccaatc atcttgccag accaatcacc ttaaccggat acactgccgg atataatccc
    82681 actaacaccc agacaccaag caccatacct gataactacc gtattcagga aggaaatgcg
    82741 agtgatttca ccgtcaccgg cacaaccgat ccgccaaaaa gaggcgtacc aatgtcgata
    82801 tagtcccccg cgcggacaat cacttcgtca atgactgcca acgggagttg ttgtagctgt
    82861 gggcgcaaca acatgcccag agctttacca aagtcctgcc cggccaccac cagcagggga
    82921 tgcggattcg gaaaacgcgc gacgaaatcg accagcgcgt tgatgaccgt cagaaccgcg
    82981 gcgtaacgca caggcagcga ggcgggaagc gccagcacgt acacgtcagt tttgggatca
    83041 agatccagct gaagcagcgc ctgttgccag gcactcacca gatccgtttc atcaatcggg
    83101 atcgccaccg gcaaattgcg cagcggcagt tgtacgccct ccagccagat tgtgctgcca
    83161 gagagcgaaa gggtatgtgc acccgcgcca atcaccgtgg agcgtacggt ttgcgccgga
    83221 aactgcacat tcatctcacg caggcgcgga tggtcatgca gcgccgttgc cagaagtggg
    83281 ccaatatcgg caaaacagaa cgggttggcg ggctggtggc gataacattc gcccacgcct
    83341 ccagaaagcg taatgatttc gggcgtaaca cctgcgggca gcaaaccggt ttgcatcaat
    83401 gcctgcgcga gcggcgaaag cgttccgtca atcacttcga caatcagcgc cgccatccgc
    83461 cgggtaacct gcaccagctg cgcgccggtc agcgaacggg cgtcagtgcc tgcaccgaag
    83521 cactcatcca caatcatctg ccccggttta tgagcgtaaa ccacgcgccc ctggctgtcg
    83581 gtttccagca ggcgaccacc gacgttgaga caggcagtgc cgctgatttt tccggcatcg
    83641 aacagggcgt agttcgcggt gccaccgccg atatcgatat tcagtacccg acacagccgt
    83701 tgttcagaaa gggtttgcgc cccggctccg tgaccggcga tcacggattc gaggtgcggc
    83761 ccggcgctgg cgacgacaaa atcgcccagc gactgttttt ccttcatcac tttttccatc
    83821 agctgtgcga ccaggctttc ggtaaactgg ccttccggca gctgggcaat gatggtttcg
    83881 cggatgcgct ggctttcggt tttgtcggct ggcacgctgg cgggcacgga cgccgttacc
    83941 gggatcgatt catcacattc ggtgatggta aaacccagta gatccgccac ttcacgggct
    84001 tccggggtaa taatgctggc gcgcagaacc acggacattg cctgttcgcc gcgcgcgtgt
    84061 gcttcacgaa tatcattcgc tgtgatgagt tttttcacct gcttcctcct gtttggtgag
    84121 agatgccaga taatccacca gttgctgcac gctttgcggg tcgtggctgt ttagctcgaa
    84181 aatcggctct tcgaaacccg tctcaagcaa taactttcgc gttgcggcga cgtcagcatc
    84241 tggcatgtcc gttttactga tgacggcgat ttgccgtttg ctgacgccaa tatccagcaa
    84301 cccggcaggc aaccgacttt ccgggtcatt tgcgccatgg acatagatca gcatgtctac
    84361 atcctgtagc gtggtaatta aggcgtgata ccagcgggga tggctaaaat attcacccgg
    84421 cgtatcaata tcgcccttat cattaaattc cacggccagt gtttttctgg cgagggtata
    84481 attcccctgc aacgcattaa ataacgttgt ttttcctgcc ccgacgctac ccacaaacgc
    84541 aattcgtttc atgaatg
//
LOCUS       DAEEGA010000002        78784 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEEGA010000002 DAEEGA010000000
VERSION     DAEEGA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78784
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.002"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..229
                     /locus_tag="KLH46_000087"
     CDS             <1..229
                     /locus_tag="KLH46_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HBE0376937.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            649..1264
                     /locus_tag="KLH46_000088"
                     /pseudo
     CDS             649..1264
                     /locus_tag="KLH46_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1367..1690
                     /locus_tag="KLH46_000089"
     CDS             1367..1690
                     /locus_tag="KLH46_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HBE0376938.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1757..3214)
                     /gene="nuoN"
                     /locus_tag="KLH46_000090"
     CDS             complement(1757..3214)
                     /gene="nuoN"
                     /locus_tag="KLH46_000090"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBE0376939.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3221..4750)
                     /gene="nuoM"
                     /locus_tag="KLH46_000091"
     CDS             complement(3221..4750)
                     /gene="nuoM"
                     /locus_tag="KLH46_000091"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBE0376940.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4914..6755)
                     /gene="nuoL"
                     /locus_tag="KLH46_000092"
     CDS             complement(4914..6755)
                     /gene="nuoL"
                     /locus_tag="KLH46_000092"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBE0376941.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVFSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6752..7054)
                     /gene="nuoK"
                     /locus_tag="KLH46_000093"
     CDS             complement(6752..7054)
                     /gene="nuoK"
                     /locus_tag="KLH46_000093"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBE0376942.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7051..7605)
                     /gene="nuoJ"
                     /locus_tag="KLH46_000094"
     CDS             complement(7051..7605)
                     /gene="nuoJ"
                     /locus_tag="KLH46_000094"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBE0376943.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7617..8159)
                     /gene="nuoI"
                     /locus_tag="KLH46_000095"
     CDS             complement(7617..8159)
                     /gene="nuoI"
                     /locus_tag="KLH46_000095"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBE0376944.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8174..9151)
                     /gene="nuoH"
                     /locus_tag="KLH46_000096"
     CDS             complement(8174..9151)
                     /gene="nuoH"
                     /locus_tag="KLH46_000096"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBE0376945.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9148..11874)
                     /gene="nuoG"
                     /locus_tag="KLH46_000097"
     CDS             complement(9148..11874)
                     /gene="nuoG"
                     /locus_tag="KLH46_000097"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBE0376946.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11927..13264)
                     /gene="nuoF"
                     /locus_tag="KLH46_000098"
     CDS             complement(11927..13264)
                     /gene="nuoF"
                     /locus_tag="KLH46_000098"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBE0376947.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13261..13761)
                     /gene="nuoE"
                     /locus_tag="KLH46_000099"
     CDS             complement(13261..13761)
                     /gene="nuoE"
                     /locus_tag="KLH46_000099"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBE0376948.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13764..15566)
                     /gene="nuoC"
                     /locus_tag="KLH46_000100"
     CDS             complement(13764..15566)
                     /gene="nuoC"
                     /locus_tag="KLH46_000100"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBE0376949.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15660..16322)
                     /gene="nuoB"
                     /locus_tag="KLH46_000101"
     CDS             complement(15660..16322)
                     /gene="nuoB"
                     /locus_tag="KLH46_000101"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBE0376950.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16338..16781)
                     /gene="nuoA"
                     /locus_tag="KLH46_000102"
     CDS             complement(16338..16781)
                     /gene="nuoA"
                     /locus_tag="KLH46_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBE0376951.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17412..18350)
                     /gene="lrhA"
                     /locus_tag="KLH46_000103"
     CDS             complement(17412..18350)
                     /gene="lrhA"
                     /locus_tag="KLH46_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBE0376952.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19270..20487
                     /gene="alaA"
                     /locus_tag="KLH46_000104"
     CDS             19270..20487
                     /gene="alaA"
                     /locus_tag="KLH46_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBE0376953.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20571..21170
                     /gene="yfbR"
                     /locus_tag="KLH46_000105"
     CDS             20571..21170
                     /gene="yfbR"
                     /locus_tag="KLH46_000105"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBE0376954.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21229..23061)
                     /locus_tag="KLH46_000106"
     CDS             complement(21229..23061)
                     /locus_tag="KLH46_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBE0376955.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23148..23798)
                     /gene="hxpA"
                     /locus_tag="KLH46_000107"
     CDS             complement(23148..23798)
                     /gene="hxpA"
                     /locus_tag="KLH46_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HBE0376956.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23809..24303)
                     /locus_tag="KLH46_000108"
     CDS             complement(23809..24303)
                     /locus_tag="KLH46_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBE0376957.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24386..24841)
                     /locus_tag="KLH46_000109"
     CDS             complement(24386..24841)
                     /locus_tag="KLH46_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBE0376958.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25179..26381
                     /gene="ackA"
                     /locus_tag="KLH46_000110"
     CDS             25179..26381
                     /gene="ackA"
                     /locus_tag="KLH46_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBE0376959.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26456..28600
                     /gene="pta"
                     /locus_tag="KLH46_000111"
     CDS             26456..28600
                     /gene="pta"
                     /locus_tag="KLH46_000111"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBE0376960.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28790..30310
                     /gene="yfcC"
                     /locus_tag="KLH46_000112"
     CDS             28790..30310
                     /gene="yfcC"
                     /locus_tag="KLH46_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBE0376961.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30343..30885)
                     /gene="yfcD"
                     /locus_tag="KLH46_000113"
     CDS             complement(30343..30885)
                     /gene="yfcD"
                     /locus_tag="KLH46_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBE0376962.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30943..31494)
                     /gene="yfcE"
                     /locus_tag="KLH46_000114"
     CDS             complement(30943..31494)
                     /gene="yfcE"
                     /locus_tag="KLH46_000114"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBE0376963.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31550..32194)
                     /gene="yfcF"
                     /locus_tag="KLH46_000115"
     CDS             complement(31550..32194)
                     /gene="yfcF"
                     /locus_tag="KLH46_000115"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBE0376964.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32330..32977
                     /gene="yfcG"
                     /locus_tag="KLH46_000116"
     CDS             32330..32977
                     /gene="yfcG"
                     /locus_tag="KLH46_000116"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBE0376965.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33034..33396
                     /gene="folX"
                     /locus_tag="KLH46_000117"
     CDS             33034..33396
                     /gene="folX"
                     /locus_tag="KLH46_000117"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBE0376966.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33417..34310
                     /locus_tag="KLH46_000118"
     CDS             33417..34310
                     /locus_tag="KLH46_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBE0376967.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34358..35248)
                     /gene="rpnB"
                     /locus_tag="KLH46_000119"
     CDS             complement(34358..35248)
                     /gene="rpnB"
                     /locus_tag="KLH46_000119"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBE0376968.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35445..36218)
                     /gene="hisP"
                     /locus_tag="KLH46_000120"
     CDS             complement(35445..36218)
                     /gene="hisP"
                     /locus_tag="KLH46_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBE0376969.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36226..36942)
                     /locus_tag="KLH46_000121"
     CDS             complement(36226..36942)
                     /locus_tag="KLH46_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0376970.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36939..37625)
                     /gene="hisQ"
                     /locus_tag="KLH46_000122"
     CDS             complement(36939..37625)
                     /gene="hisQ"
                     /locus_tag="KLH46_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBE0376971.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37715..38497)
                     /gene="hisJ"
                     /locus_tag="KLH46_000123"
     CDS             complement(37715..38497)
                     /gene="hisJ"
                     /locus_tag="KLH46_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBE0376972.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38718..39500)
                     /gene="argT"
                     /locus_tag="KLH46_000124"
     CDS             complement(38718..39500)
                     /gene="argT"
                     /locus_tag="KLH46_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBE0376973.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39766..40335)
                     /gene="ubiX"
                     /locus_tag="KLH46_000125"
     CDS             complement(39766..40335)
                     /gene="ubiX"
                     /locus_tag="KLH46_000125"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBE0376974.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40430..41947)
                     /gene="purF"
                     /locus_tag="KLH46_000126"
     CDS             complement(40430..41947)
                     /gene="purF"
                     /locus_tag="KLH46_000126"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBE0376975.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41984..42472)
                     /gene="cvpA"
                     /locus_tag="KLH46_000127"
     CDS             complement(41984..42472)
                     /gene="cvpA"
                     /locus_tag="KLH46_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBE0376976.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42731..43393)
                     /gene="dedD"
                     /locus_tag="KLH46_000128"
     CDS             complement(42731..43393)
                     /gene="dedD"
                     /locus_tag="KLH46_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBE0376977.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43383..44651)
                     /gene="folC"
                     /locus_tag="KLH46_000129"
     CDS             complement(43383..44651)
                     /gene="folC"
                     /locus_tag="KLH46_000129"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBE0376978.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44721..45635)
                     /gene="accD"
                     /locus_tag="KLH46_000130"
     CDS             complement(44721..45635)
                     /gene="accD"
                     /locus_tag="KLH46_000130"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBE0376979.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45791..46450)
                     /locus_tag="KLH46_000131"
     CDS             complement(45791..46450)
                     /locus_tag="KLH46_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0376980.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46533..47345)
                     /gene="truA"
                     /locus_tag="KLH46_000132"
     CDS             complement(46533..47345)
                     /gene="truA"
                     /locus_tag="KLH46_000132"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBE0376981.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47345..48358)
                     /locus_tag="KLH46_000133"
     CDS             complement(47345..48358)
                     /locus_tag="KLH46_000133"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBE0376982.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48424..49560)
                     /gene="pdxB"
                     /locus_tag="KLH46_000134"
     CDS             complement(48424..49560)
                     /gene="pdxB"
                     /locus_tag="KLH46_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBE0376983.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49659..50654
                     /gene="flk"
                     /locus_tag="KLH46_000135"
     CDS             49659..50654
                     /gene="flk"
                     /locus_tag="KLH46_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBE0376984.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50651..51829)
                     /locus_tag="KLH46_000136"
     CDS             complement(50651..51829)
                     /locus_tag="KLH46_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0376985.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52084..53325)
                     /gene="fabB"
                     /locus_tag="KLH46_000137"
     CDS             complement(52084..53325)
                     /gene="fabB"
                     /locus_tag="KLH46_000137"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBE0376986.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53484..55490
                     /gene="mnmC"
                     /locus_tag="KLH46_000138"
     CDS             53484..55490
                     /gene="mnmC"
                     /locus_tag="KLH46_000138"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBE0376987.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55611..55889)
                     /locus_tag="KLH46_000139"
     CDS             complement(55611..55889)
                     /locus_tag="KLH46_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBE0376988.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55923..56471)
                     /gene="epmC"
                     /locus_tag="KLH46_000140"
     CDS             complement(55923..56471)
                     /gene="epmC"
                     /locus_tag="KLH46_000140"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBE0376989.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56471..57280)
                     /locus_tag="KLH46_000141"
     CDS             complement(56471..57280)
                     /locus_tag="KLH46_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBE0376990.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57280..58104)
                     /gene="mepA"
                     /locus_tag="KLH46_000142"
     CDS             complement(57280..58104)
                     /gene="mepA"
                     /locus_tag="KLH46_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBE0376991.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58108..59193)
                     /gene="aroC"
                     /locus_tag="KLH46_000143"
     CDS             complement(58108..59193)
                     /gene="aroC"
                     /locus_tag="KLH46_000143"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBE0376992.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59228..60160)
                     /gene="prmB"
                     /locus_tag="KLH46_000144"
     CDS             complement(59228..60160)
                     /gene="prmB"
                     /locus_tag="KLH46_000144"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBE0376993.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60326..60877
                     /gene="smrB"
                     /locus_tag="KLH46_000145"
     CDS             60326..60877
                     /gene="smrB"
                     /locus_tag="KLH46_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBE0376994.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60981..61871)
                     /locus_tag="KLH46_000146"
     CDS             complement(60981..61871)
                     /locus_tag="KLH46_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBE0376995.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61858..62382)
                     /locus_tag="KLH46_000147"
     CDS             complement(61858..62382)
                     /locus_tag="KLH46_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0376996.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62379..62849)
                     /locus_tag="KLH46_000148"
     CDS             complement(62379..62849)
                     /locus_tag="KLH46_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0376997.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62846..63394)
                     /locus_tag="KLH46_000149"
     CDS             complement(62846..63394)
                     /locus_tag="KLH46_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0376998.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63399..64121)
                     /locus_tag="KLH46_000150"
                     /pseudo
     CDS             complement(63399..64121)
                     /locus_tag="KLH46_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64141..64863)
                     /locus_tag="KLH46_000151"
     CDS             complement(64141..64863)
                     /locus_tag="KLH46_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBE0376999.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64923..66155)
                     /locus_tag="KLH46_000152"
     CDS             complement(64923..66155)
                     /locus_tag="KLH46_000152"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBE0377000.1"
                     /translation="MRADWQTNYQHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLK
                     AKLALGEDYLRSDIFDGFNYVGGSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQM
                     GRVIYETQVPAGPFRIQDLGDSVSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVR
                     YKIMMGRPQEWGHHVEGEFFSGAEASWGIANGWSLYGGALGDENYQSAALGVGRDLST
                     FGAVAFDVTHSHTKLDKDTAYGKGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENF
                     MTMSEYLDASDSGMVRTGNDKEMYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYN
                     IMLSHYFNMGSIRNVSISMTGYRYEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGR
                     TAVRSVISAVSMTRLTIS"
     gene            complement(66204..66782)
                     /locus_tag="KLH46_000153"
                     /pseudo
     CDS             complement(66204..66782)
                     /locus_tag="KLH46_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499769.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66864..67427)
                     /locus_tag="KLH46_000154"
     CDS             complement(66864..67427)
                     /locus_tag="KLH46_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE0377001.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(68111..68596)
                     /gene="sixA"
                     /locus_tag="KLH46_000155"
     CDS             complement(68111..68596)
                     /gene="sixA"
                     /locus_tag="KLH46_000155"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBE0377002.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(68799..70943)
                     /gene="fadJ"
                     /locus_tag="KLH46_000156"
                     /pseudo
     CDS             complement(68799..70943)
                     /gene="fadJ"
                     /locus_tag="KLH46_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
     gene            complement(70943..72253)
                     /gene="fadI"
                     /locus_tag="KLH46_000157"
     CDS             complement(70943..72253)
                     /gene="fadI"
                     /locus_tag="KLH46_000157"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBE0377003.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72433..72717)
                     /locus_tag="KLH46_000158"
     CDS             complement(72433..72717)
                     /locus_tag="KLH46_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBE0377004.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            73089..74429
                     /gene="fadL"
                     /locus_tag="KLH46_000159"
     CDS             73089..74429
                     /gene="fadL"
                     /locus_tag="KLH46_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBE0377005.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            75180..75854
                     /locus_tag="KLH46_000160"
     CDS             75180..75854
                     /locus_tag="KLH46_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377006.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLFLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTKYQNEINQLSALG
                     VNFDDI"
     gene            complement(76036..76791)
                     /gene="mlaA"
                     /locus_tag="KLH46_000161"
     CDS             complement(76036..76791)
                     /gene="mlaA"
                     /locus_tag="KLH46_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBE0377007.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            77085..78017
                     /locus_tag="KLH46_000162"
     CDS             77085..78017
                     /locus_tag="KLH46_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBE0377008.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            78091..78165
                     /locus_tag="KLH46_000163"
     tRNA            78091..78165
                     /locus_tag="KLH46_000163"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78124..78126,aa:Arg,seq:cct)
     gene            78327..>78784
                     /gene="intS"
                     /locus_tag="KLH46_000164"
     CDS             78327..>78784
                     /gene="intS"
                     /locus_tag="KLH46_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBE0377009.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT        19966 a        21039 c        19327 g        18452 t
ORIGIN      
        1 ggaaagcaat tctgacagcg ccccctatac gttattaaaa aactatgctg acagatttaa
       61 aaaatattca gcagaagagc agtacgaaat tgattttcaa catcgactgg taaacagaaa
      121 ttgttattta gataaatatg gcgatgcacg aatcaatgct tcttatacac aattagaaat
      181 aaaacactca caacccaaaa atagagcatc cggcaaaaga gtgagttaac atcattgtta
      241 atcaattcga ccagtcagta aatatgctga ctggtcgaac ataatttgct aaaaagtcat
      301 cactgactga tgtcagtccc accatctgcc agttcaatct acgggtattt ccagcatatc
      361 gcggcacgcg attgctggtc tgttgatgta gtggattgta gttgctgact tttcttaccc
      421 tcagatttct gccccacttg tcaattcaat ctgccaataa ataacataat tatattttta
      481 ttcctcacag aaattccttt ttaaaaatag cgaacccact gtaaaaaaca ctttttgtat
      541 acctctacca agcgtaataa aataaacgct cataattcat tataaataat aatgaaataa
      601 cgccttttat tgataattta catatgaaaa aaattttcct tttgttatat tatttctgtt
      661 ttcactccct ctgattattt ttaccaaccc tgggtcactg cattatcgcc aacgccccga
      721 cacgtaagtc ctgagcaatt agaaaaaaca gttcgttatc ttacacaaac tgtgcatcca
      781 cgtaatgccg gcaatattga taatctgaat aggtccgcag agtatataaa agagttcttt
      841 gtcagtagcg gtgccagagt taccgcgcag ggcgtccccg attggctatt acgactctgc
      901 gcctggaagc cagaattacc cttacccggc aatgtcctgg ctttatcccg atcggggaga
      961 ctttattgct gtggtcggca gaatgcagga tatcaacgcc gttcgtcagg taaaagcggc
     1021 attgttgtca tcccgggatt tatctgttta ttctataaat gccccagggt ttattcccgg
     1081 tattgatttt tctgatcacc tcaattattg gcaacacgat attcccgccg taatgattac
     1141 tgacaccgct ttttatcgta ataaacaata ccacttgccc ggtgataccg cagacagatt
     1201 gaattatcag aaaatggctc aggtggtaga tggtgttata accttgttat acaacagtaa
     1261 ataataaaaa tttctcgagg cgggtccaga aacagcgaca tgtacatttt attttccctc
     1321 gtctgtttta agatgcgaaa cataaaattc aatggaggaa aaataaatgg gaatgattgg
     1381 ctattttgca gaaattgact cagaaaagat taaccaactg cttgagtcta ctgaaaaacc
     1441 tttgatggat aatatacatg acacgctttc aggattacgc cgactggaat cactggatcg
     1501 aagtgagctg cgtaaacagt tttccattaa gcgactcaat gaaatggaga tttatccggg
     1561 agtgaccttt tcagaagagt tagaggggca actctttgcc tcgatcatgc ttgatatgga
     1621 aaagctgatt agcgcgtatc gacgtatgct gcgacaaggg aatcatgcgc tgacggttat
     1681 tgttgggtga tggtgtcaga gcgccaaaaa aaaccgccga acatgtcggc ggctttctga
     1741 cttacaaact aacagtttac atcagcggca ttgctaaccg cacaatacta atcagcggtt
     1801 gtggccatac acccagcacc agtaccaaca gtgcagaaat cagcaccacg ataccgcccg
     1861 cgctgtactg ccagtttgac ggtgcatcgc gacccggttg ttccggtgcg tgcaggtaca
     1921 ggctcaccgc cacgcgcagg tagtagtaga ggccgattgc cgaaccgaca accacggcac
     1981 ccaccagcca ccacaagtgt gcctggacac cgaccgccag cacgtagaac ttaccgataa
     2041 agcccagcgt catcgggata ccggccagag acagcatcat caccgtcatc actgccgcga
     2101 ggatcggacg atgccagaac agaccgcggt aagagaacag ggaatcagca tccgggccac
     2161 gatacgggct ggacatcagg ctgaccacgc cgaacgcgcc gaggctgctg aacagataac
     2221 cggccaggta aacccctacc gcttccatcg acatctcgcc ggtttgcagc gcaatcagcg
     2281 ccaccagcag atagccgagg tgagagatag atgagtaacc gagcagacgt ttgatgttgg
     2341 tctggctcaa cgccatcagg ttaccgaaga tgatggaggc aaaggcgata atcgccagta
     2401 ctacgcgaat cgcttcgctg tcacccaccg gtgcgtagag gaacagacgc atcaccacac
     2461 cgaagatagc gattttgctc gccgtcgcca ggaaagtgga aaccggcgca ggcgcgccct
     2521 ggtatacgtc tggcgtccac aggtggaacg gcaccagaga gagtttgaag ccgaggccaa
     2581 caatcatcat gccgaaacct gccagcaaca gcggctcgtt gagcataccg tcgccgaggt
     2641 ttttacccag cgcgacaaac gacaggtcgc cagactgcgc atacaccagc gccataccga
     2701 acagcaggaa agaagacgct gcggcagaaa ggatggtgta tttgatactg gcttccagcg
     2761 aacgtttctg gcggaaagcg taaccgacca ggccaaacag cggcaaagag atcagttcaa
     2821 taccgaggaa cagagacgcc agatggtttg cattcgccag caggatcccg cccagcgcgg
     2881 caattaacac caacaggtag aactcatcct tgttgtcgtt atagccttca agccacgggt
     2941 aggcgaaagt acaggtggcg aggctcgcca acaataccag cccggtgtaa agcatggcga
     3001 aaccatcaac gcgcatcagc ggcgtaacgt ccatagcgcc cgcctggcca acaaaccaga
     3061 gcgaaaccag cgccgcgtta agcccaataa ccgagagcgt agcgttgagg aaatgattgc
     3121 gtcgccacgc aatggagagc atcacaacca ccaccgtcaa gccgacgatc agcaacggta
     3181 gcagtgcgat caggttttgt ggagttattg tcatggcgat ttacggcctt gtagtagtaa
     3241 cggaattaac aaaccactgc tggatattgc caatcgcgga gtgcgaggta tccagaatcg
     3301 gctgcggata gaagcccagc agtaccagca gcaccaccag caacaggatc ataaacagct
     3361 cacgcagcga catccctggc agttcctggc tggcaatctg gcttttcgct ttaccgaagt
     3421 aagcgcgatg caacatcgcc agcgaataaa cagatgcaaa gaccagccca aaggtagaga
     3481 taacggtaat caccgggaca acctggaagc tgccgaacag aatcataaat tcgccgacga
     3541 agttaccggt gccaggcatc ccaagcgttg ccaccgcaaa gaacagcgac agtgctggca
     3601 gccatttcat cttgctccac agaccgccca tcatgcgcat gtcgcgggta tggatacgtt
     3661 cataaagctg accgcagaga ataaacagac ccgccgccga caagccgtgc gcaatcatct
     3721 ggattaccgc gccctggtag gccaactggc tgccggtgta gattgcaatc agcacgaagc
     3781 ccatgtggga aaccgaggtg taggcgatca gacgtttgat atcggtctga gcgaaggtca
     3841 tccacgcacc gtagaagatg ccgataacac ccagccacat agcgattggc gcgaactctg
     3901 ccgacgcgtt cgggaacagc ggcagggaga aacgcaacaa accgtaagcg gcggttttca
     3961 gcaagatccc cgcgaggtcc acggaaccgg cggtcggagc ctgggagtgc gcatccggca
     4021 gccagccatg cagcggaacc accggcattt tgactgcgaa ggcgatgaag aagcccagca
     4081 tcaacagata ttccacacca ctggacattg gcgtattcag cagctcttca tagttgaagg
     4141 tccagacgcc ggtcgcattg tagtgaacaa aaaccagcgc caggatggcg atcaacatca
     4201 ccagaccact cgcctgggtg taaatgaaga acttggttgc cgccgtgata cgcgttttac
     4261 cgtcagaggc tttatgcccc cacagtgcga tcaggaagta catcggcacc agcatcattt
     4321 cccagaagaa gaagaacagg aacatgtcga tggcaaggaa cacgccgata acgccgccca
     4381 ggatccacat caggttgagg tggaagaagc cctgatattt ttcgatctct ttccacgaac
     4441 atagtaccgc cagcacaccg agcagaccgg tcagcacgac catcagcagc gacagaccgt
     4501 caatggcgag atgaatagag ataccaaaac gcgggatcca cggcatgtcg aattcagact
     4561 gccactgcgg aattccggcg gattgcgtca gtgaataacc gccctgcaac cacagttgca
     4621 gcgacagcgc cagcgtcaat cccatggtga tcagcgcgat ccagcgtggc accttgacgc
     4681 caaagcgttc ggtctgccag cacaggaagc cgccaataaa gggaattaat attagccagg
     4741 gtagtaacat ggcgatcttt attccttgta aaagtcccgt caggaccgga ttttcaacga
     4801 attctcacga cagaaaattc tcttcggact gggggtagtg tcggatgcgc cgcgaaggca
     4861 gcttatccga cctacgttct tatatgccca ggggcaatcc cacaatcctt aactcaacgc
     4921 agtaccatca acagtgccag caccacgacc gcaccgatgc tcatggatgc cacataccag
     4981 cgcagataac cgttctcgct taacagcaga cctttacctg caaagcggga aaaaaccgcc
     5041 gggatgttca tcattgagtt cagcggatcg cgtttcagca accaggcaat acccaggaac
     5101 ggcttgacga acactttgtc gtacagccag tcaaagcccc aagcgttgta ccaccaggta
     5161 ctcagcaggc ggcccggcgc actgttggcg atggaggtca ccagagaacg tttacccagc
     5221 cacagccagg ctgccagcag aatgccgacc accgcgacca cgccagaggt aatttccagg
     5281 gtcaacatgc tgccgtgcgc cagttccgtc gtttgcggaa gcacgccctg cagcggcggt
     5341 acaatcagtg cgccaacgaa ggtggaaagg atcagcagca caatcagcgg caggctgtga
     5401 gttacccctt tcacggcgtg agcgtgaatt tgttcttttc cgtggaagac gatgaaaatc
     5461 atacggaagg tgtagagcga ggtcataaac gcaccgacca gacctgccac catcagattg
     5521 atatgaccat tcgccatcgc acccgcgagg atctcatcct tactgaagaa gcccgcagtg
     5581 accagcggca gtgccgacag tgctgcgccg cccaccagga agcagagata aaccagcgga
     5641 atagatttac gcagaccgcc catcttgaag atgttctgtt cgtgatggca ggccagaatg
     5701 acggaaccgg atgccaggaa cagcagcgct ttaaagaacg cgtgagtcat caagtggaaa
     5761 atcgccgcat cccatgcctg cacgccaagc gcgaggaaca tgtagccaat ctggctcatg
     5821 gtagagtaag cgagaacacg tttgatgtcg gtctgtacca gcgcggcaaa accggccagc
     5881 agcagcgtaa ccgccccgac aatacccacc agatgcagaa cttccggcgt catcaggaac
     5941 aggccgtggg tacgggcgat caggtagaca cccgcggtta ccatcgttgc ggcgtggatc
     6001 agcgcggaga caggcgtcgg gcccgccatc gcgtcagcaa gccatgtctg caacggcaac
     6061 tgcgcagatt taccgaccgc accgcccagc agcatcagcg tcgcccacat cagcatgtta
     6121 ttgccgtcag caaagtgcgc tggtgccagt tccaccattt cgcggaagtt cagggtgccc
     6181 agttcgttgt aaagaatgaa cagtgcgaaa gcgaggaaaa cgtcacccac acgggtcacg
     6241 acgaacgctt tcattgctgc tgcgccattc ttcggatcgg tgtaatagaa accgatcagc
     6301 agataggagc acaggcccac gccttcccag ccgaggtaca tcagcagcag gttatcggcg
     6361 agaaccagaa ctaccatgct ggcgatgaac aggttggtgt aggcgaagaa gcgagagtag
     6421 ccctcttcac cgcgcatgta ccaggaggcg tacatgtgaa taaggaaacc cacaccagtg
     6481 accaccgaga gcatggtcag cgacaggccg tccagcacca ggttaaaacc gatgttaaag
     6541 tcgcctaccg acatccacgt ccacagcggc tggctgtatg cctgctcgcc gttagcgaag
     6601 aaatcaacgc cgataaaggc ggttaccagc gccgccaggc ccacagagcc aacgccgacg
     6661 atcgccgaaa cgttttcaga ccagcgccca cgggagaacg ccagcaggac gaagccaatc
     6721 aatggcaaaa taatggttaa ggcaagcatg ttcatccgcg catctcactt actgaatcga
     6781 tgttcaggtt ctggcgacga cggtgaagtt gcagcagcag cgcaaggccg atactcgctt
     6841 ctgccgccgc gaggctgatg gcgagaatgt acatcacctg accgtcggtc tgaccccagt
     6901 agcttccggc gaccacaaag gccagcgcgg aggcgttaat catgatttcc agaccaatca
     6961 gcataaacag cagattgcga cggataacca gaccggttaa gccaagaacg aataagattg
     7021 ccgcgaggat cagtccatgt tgtaagggga tcatgcgtgc tcctccgttt ttcttttcgc
     7081 gctgtcgtct ttacgattgc tcagcacttc acccgcacgc tcttcacgac cgacgtggaa
     7141 ggccacaacc agacccgcga gcagcagcat agaagccagt tccaccgcca gtacgtaagg
     7201 cccgaacagc gtaataccca ctgctttagc actgattggc gtaccgtcga taccctgatc
     7261 gttaacaccg aggatggcgt aaacaatcac caccagcatg atggccgaca aaattgccgg
     7321 gccaatccac acctgcggtt tcagccactg gcgttcctgt tcgatttctg aaccgcccag
     7381 gttgagcatc atcaccacga acacgaacag caccataatg gcacccgcgt agacgataat
     7441 ttccagcgca ccggcgaagt aagcgcctag tgagaagaac accccagaaa tcgccagcag
     7501 cgaaataatc aggtacagca gtgcgtgtac cggattggta tgggtgatca ctcgcaaggt
     7561 cgcaagtatg gctatcaggc cacagatata aaaagcgaac tccattgccc ctctccttac
     7621 ggtaacaggc tcttgacgtc gataggcttg gcttcgttct ctgcttcgcc cttatctttg
     7681 ccgtcgattg ccatacctgc catccggtag aagttatatt ccgggtattt gcccggaccg
     7741 gagatcagca gatcctcttt ctcgtaaacc agatcctggc gcttgtattc ccccatttcg
     7801 aaatccgggg ttaactgaat cgccgtggtc ggacaggctt cttcgcacag accacagaaa
     7861 atgcagcgtg agaagttaat gcggaaaaat tccggatacc agcgaccgtc tttggtttct
     7921 gctttttgca gcgagataca gccgaccggg caggctaccg cacagaggtt acaggctacg
     7981 caacgctctt cgccgtccgg gtcgcgggtc agaacgatac gaccacgata acggggcggc
     8041 agatagaccg gctcttccgg gtacattcgc gtttcgcgtt tggcgaacgc gtgcaggccg
     8101 atcatccaga tactacgaac ctgggtgccg aaacctacta acaattcttt taaggtcatg
     8161 gtcttattgc cccttattgc gcctgccaga gaatgacagc cgccgttacc agcaagttga
     8221 tcagcgtcag cggcaggcag attttccagc cgaaggacat tacccggtca taacgcggac
     8281 gcggtaacga cgcacgaatc aaaatgaaca tcatcataaa gaacgcggtt ttcagcgcga
     8341 accagatgaa tggcggtaac aacgggcctt gccagccacc gaagaacagc gtcaccatca
     8401 atgcagagat ggtcacaatc ccgatgtact cacccacgaa gaacagaccg aacttcatac
     8461 cggaatattc aatgtggtaa ccatccgcca gttcctgctc ggcttccggc tggtcaaacg
     8521 ggtgacggtg acacaccgcc acgcccgcga tggcaaaggt aataaaacca aagaattgcg
     8581 ggatgacgtt ccacacatgc gcctggctgt tgacgatgtc ggtcatgttg aatgaaccgg
     8641 cctgcgccac cacgcccatc agggaaagcc cgaggaacac ttcgtagctc agggtctgcg
     8701 cagaagcacg catcgcaccc agcagcgagt atttgttgtt acttgaccag cccgcaaaca
     8761 gcaccgcgta aaccgccaga cctgccatca tcaggaagaa caaaatcccg atgttcaggt
     8821 cggcaaccac ccaacccgga ctgactggca caattgcaaa ggccagcagc agcgaggtaa
     8881 aggcaatcat cggtgccagg gtaaagatga cgcgatccga gaatttcggg atccagtctt
     8941 ctttaaagaa cattttgatc atgtccgcaa ccagctggag cgaaccgccc cagccaacac
     9001 ggttaggtcc ataacggttc tggaacagac ccagcaggcg acgttcgcca aagctcatga
     9061 atgccccaca ggtgacaacc accagcagga tcaccaccgc tttgaggatg gtcagcagga
     9121 tctcaatcag ttccggtgat atccaactca ttgttgtgcc tccttgagat cctcaagatg
     9181 cgcgccagcc agcaccggag caatgccgga catacccatc ggcaagccca cctgccctgc
     9241 cgtcagtcct tcggcgattt caaccggcag cgtgaccgtg ttgccatcgt aactaaagga
     9301 gacgcgtgta cctgcgttca cacccaactt cgcggcatcc gctgggttga gtttgatgta
     9361 cggctgcggc atacggctct ggaagaccgg agcacgctgt gacaattcat cgctgccaaa
     9421 caggtggtag tacggcgcga tacgccattt cccgtcctgc ggctggaagc gtgccggtac
     9481 gctggtgaag taatccagac cgttttcgct ggtttcaaac agacgcacgc ccggatcgcc
     9541 aaagcgcagt ttgccgccca cttcgtcctg gaatttgttc cacgcctgcg gggagttcca
     9601 gcccggcgcc caggcaaacg gcacttgcga acggtgcgcg gtcggctggt tgttaccttc
     9661 catcgagaag gtgaacatgg tgtcaatatc ctgcggctga cgcggctcat gaacgctgat
     9721 attggcgcgc atggcggtac gaccgctgta acggtgcggt tcacgggcca gtttctgccc
     9781 acgaatacgg aatgtcgcat ccggcgcagc atctttgata cccgccagtt ccgggatttt
     9841 cgccacaaca gcgtcaatta catggtcgag ctgcgtccag tccacttcac ggctcagcaa
     9901 ggtgctgtgc agcgagtgca gccagcgcca gctttccagc atgacagttt tgctgtcgta
     9961 ataagccgga tcgtaaacct ggaagaaacg ttgggcgcga ccttcattat taattaccgt
    10021 accgtcgctt tcagcaaagc tggcagagga aagcaccaga tgggcgtttt ccatgatcgc
    10081 tgtgcgttga tgatcaacca ccatcaccag cggcgcttta gccagcgcag cattcacgcg
    10141 ggtagcagaa gcgtaacgat gcagatcgtt ttccaacacc accaccgcgt cggcgcgtcc
    10201 ggtttccagt tcggttaacg cttcttcaag cgaaccgcca cccataatgc ccagccccat
    10261 gctgttgacg gaacgggcaa tcatggtgat accaacgtca gcgccgcgac ctttcagggc
    10321 tttcgcgacg ttagccgcag cctgaatcac ctctatgcta ccggcgttcg tcccggagat
    10381 aatcaacggt ttcttcgcac ctgccagtgc ctgcacgatg acgtcgattt tgttttgcag
    10441 ctcaggttcg ataccgtcaa ccgctggtgc ggagttatcc agcgcatggg cgatggcaaa
    10501 acctaaacgc gcctgatctt caaccggtgc gcggtaagtc cacgccgcga tatcatccag
    10561 acgagtgtca tcaacgttgg taacaaacag cggatgcttc gcacgttgac cgatgttgag
    10621 gattgccgca atctgccagt cagccacttt ctgtgctgca gccatttcgc gcgctttacc
    10681 tttcaccgcc tgacgcactg ccagtgcgac gcgcgcgccg gtctgggtaa cgtcttcgcc
    10741 cagcaccagt accgcatcgt aagattcgat ttcgcgcaga gccggagtat aaatgccgcc
    10801 ttcacgcagc actttcagcg ccagttgcag acgttcctgc tcaccgtgag cgataccggt
    10861 gtagaagttt tcttcgccca ccagttcacg cagcgcaaag ttgctttcca cgctggcacg
    10921 cggagaacca ataccgatca ctttcttcga ctgacgcaga atatctgccg cgccctgcat
    10981 tgcctgttcg gcgttgaggg taatgaaatc atcgccacga cgctgtactg gctgacgcgg
    11041 acgatccttc aggttgacgt aaccgtaacc gaaacgacca cggtcgcaga ggaagtagtg
    11101 gtttaccgta ccgttgtaac ggttttcgat acgacgcagt tcgccgtaac gttcaccggg
    11161 gctgatgtta cagccgatgg aacattgctg gcagatgctc ggcgcaaact gcatatccca
    11221 tttacggttg taacgctcgg agtgcgtttt gtcggtaaat acgccggtcg ggcaaatttc
    11281 gaccaggtta ccggaaaatt cgctttccag cgtgccgtct tccgggcgac cgaagtagac
    11341 gttgtcgtgc gcgccataaa cgcccagatc tgtaccgtca gcgtaatctt tgtagtaacg
    11401 cacacagcgg tagcaggcga tgcagcggtt catttcgtga gagatgaatg gccccaaatc
    11461 ctgattacgg tgggtacgtt tggtgaaacg gtagcgacgg aagctgtgtc cggtcatcac
    11521 ggtcatatcc tgaagatggc agttaccgcc ttcttcacat accggacagt cgtgcgggtg
    11581 gttggtcatt aaccactcga ccacactttc acggaactgt ttcgcttctt cgtcgtcaat
    11641 ggaaataaag gtgccatcgg aagccggtgt catacaggac atcaccaggc gaccacgcgt
    11701 gtcttccgcg ttttggtatt gcttcaccgc acactggcgg caagcaccga cgcttcccag
    11761 cgccggatgc cagcaaaagt aaggaatatc aaggcccaga gacagacaag cttccagcag
    11821 gttgtccgct ccgttgacct cgtattcttt gccgtctaca tgaattgtag ccattagcat
    11881 gcttccagtt ttctcggtca gggaccgagc gttaatcgaa attcggttac cagcgctctt
    11941 tcagcaggtt cggctgaatc ccattaatca aatgggtatt gctgaacggc tgtttgattc
    12001 ccgcctcaaa ttcttcgcgg aaatatttga tggcgctctg taacggctcc actgcaccag
    12061 gtgcgtgggc acagaaagtt ttacccgggc ctaagaatcg acacagttgc tcaagtgttt
    12121 cgatatcgcc cggctggcct tcgccacgct ccagcgcacg cagaattttc acgctccacg
    12181 gcagaccgtc gcggcacggc gtacaccagc cgcaggactc acgggcgaaa aactcttcca
    12241 ggttacgcac cagcgacacc atgttgatct catggtcaac cgccatcgcc agcgccgtac
    12301 ccagacggct gcccgcttta ccgatacttt cgaattccat cggcagatca aggtgcgctt
    12361 cggtcaggaa gtcagtcccc gcgccgcctg gctgccaggc tttaaatttc agaccatcac
    12421 gcataccacc ggcgtaatct tcgaggatct cgcgtgcggt ggtgccgaac ggcagttccc
    12481 acagtcccgg atttttcacc cgaccggaga agcccatcag cttggtgcca gcatctttac
    12541 ttttcgagat gttctgatac cactccacgc cgttagcgag gatcgccgga acgttacaca
    12601 gggtttcgac gttgttgaca caggtcggtt tgccccatac gccggaggtt gccgggaagg
    12661 ggggtttcga gcgtgggtta gcacgacgcc cttccaggga gttgattaac gctgtttctt
    12721 ccccgcagat gtagcgccct gccccggtat ggacgaacag ttcgaagtcg aaacctgttc
    12781 ccataatgtt tttgccaagc aagcccgctt cagtggcttc ggcaatggcg cggcgcagat
    12841 ttactgccgc ttcgatatat tcgccacgca ggaagatgta gccacggtaa gctttcagcg
    12901 caaacgcgga gatgagcata ccttccacca gcaggtgcgg cagttgctcc atcaacaggc
    12961 ggtctttata ggtgcccggc tccatttcat cggcgttaca cagcaggtaa cggatgttca
    13021 tggattcgtc tttcggcatc aggctccact tcaggccagt cgaaaagccc gcgccgccgc
    13081 gccctttcag accagcgtct tttacctgat taacgatttc gtccggagac agcccggtca
    13141 gcgccttacg cgccccttcg taaccgtttt tgctgcggta ttcgtccagc cacactggct
    13201 gtttgtcatc gcgcagacgc caggtcagcg gatgcgtttc gggagtacgg ataatgtttt
    13261 tcatttatac cgctccagca gttcagggat cgcttccggg gtcagatgcg cgtgagtgtc
    13321 ctcatcgatc atcatgtttg gccctttatc acagttcccc aggcagcaag ttggcagcag
    13381 cgtaaagcgg ccatcaaatg tcgtttgccc tggtttgatg ttcagctttt tctcgagcgc
    13441 cgcctgaata ccctgatacc cgttgatatg acagaccacg ctgtcgcaat aacggatcac
    13501 atggcgacca accggctggc ggaagatctg actgtagaac gttgccacac cttcgacgtc
    13561 gcttgccgga atacccagca catcggcgat cgcgtggatc gcaccatccg gcacccagcc
    13621 acgctgcttc tgaacgattt tcagcgcttc aatggacgcc gcacgcgggt cttcgtagtg
    13681 gtgcatctcg tgctcaatcg cttcacgctc tgccgcactc agctcaaaag cctcggtttg
    13741 tggttgttga ttctcgtgca taattagcgg tccacatctg acataacaaa atcgatactg
    13801 cccagataaa caatcaggtc agacaccagg ctgccgcgga tcgccgccgg aatttgctgc
    13861 aaatgcgcat aactcggcgt gcggatacga gtgcgataac tcatggtgct accgtcgctg
    13921 gtcaggtagt aactgttgat ccccttggtc gcctcaatca tctggaaaga ttcattggca
    13981 ggcatcaccg gcccccacga cacttgcagg aagtgggtga tcagggtttc gatatgttgc
    14041 agcgtgcgct ctttcggcgg cggcgtggtc agcgggtgat ccgctttgaa cgggccttcc
    14101 ggcatgttgt tgaggcactg ctcaagaatg cgcagactct ggcgcagctc ttccactttc
    14161 agcattacgc gggtgtagca gtcagaaaca ccaccaccaa ccgggatttc aaagtcgaag
    14221 ttttcatagc cagaataagg acgcgcctta cgcacgtcga agtcgatccc ggtagcacgc
    14281 aggcccgcgc cagtggtgcc ccactccagc gcctctttcg cgccataggc ggcaacgccc
    14341 tgggaacgac ctttcagaat ggtattttgc agcgccgctt tctcgtaaga cgccagacgt
    14401 ttcggcatcc agtcgaggaa ctcacgcagc agacgatccc agccgcgcgg caggtcgtgc
    14461 gctacgccgc caatacggaa ccacgccggg tgcatacgga aacccgtgat cgcttccacc
    14521 agatcgtaaa ttttctgacg atcggtaaag gcgaagaaca cgggcgtcat tgcgccgacg
    14581 tcctgaataa aggtcgagat gtacagcagg tggctgttga tacggaacag ttcggagagc
    14641 ataacgcgaa tgacgttaac gcgatccggc acggtgatcc cggccagttt ctctaccgcc
    14701 agcacgtaag gcatttcgtt aacgcagccg ccgaggtatt caatacggtc ggtatacgga
    14761 atgtagctgt gccaggactg gcgctcgccc attttctccg caccacggtg gtggtagccg
    14821 atgtctggta cgcagtcgac aatctcttcg ccgtcgagtt gtaaaacgat acggaaagcc
    14881 ccgtgcgccg acgggtggtt cggaccgagg ttgaggaaca tgaagtcctc gttttcggtg
    14941 ccgcgcttca tcccccactc ttccggtttg aaggtcaggg cttccatctc cagatcctgt
    15001 ttggctttgg tcagctcaaa cggcgagaat tcggtagcgc gcgccgggta atctttacgc
    15061 agcgggtgac ctttccaggt ttgcggcatc atgatgcggc gcaggttcgg gtgaccgtcg
    15121 aaagtaatgc caaacagatc ccaggtttca cgctcatacc agttagcgtt cgggaacagt
    15181 ttggtgaagg tcggtacgtg caggtcgttt tctgccagcg ccaccttcag catgatgtcg
    15241 cggttacgat cgatagaaat cagatggtag aaaacggaaa aatccgcggc aggtaaccct
    15301 tcgcggtgtg tgcgcagacg ttcgtccatg ccgtgtaagt caaacagcat gacgtaaggt
    15361 ttcggcagtt tctttaagaa atcgccaact tccagtaatt gttcacgctt gatccacaca
    15421 acgggaaccc cggtgcgagt cgcctgaaca gtaaaggcat ccggcccaaa acggttgcgc
    15481 agttcgccaa ttaccggatc atcaagatga tcgcgggtct gccaggcggg ttcttgcgcg
    15541 gttaagtcgg tcatattgtt caccattgca aatggtccgt ggtgactgtc ggcgcagtaa
    15601 gcttcgcgaa atagatttag gaataagcga agaaaaatcc ctttgccgac aggcgcaaat
    15661 taaatctcgt caggtgtacg caggttggtt acggcaatgc gttcaccgcg cttgcgttcg
    15721 cgctctgatt gcatattggc gcgataaacg ccctgatcgc caaccaccca ggagagcgga
    15781 cgacgttctt tgccgataga ttcctgcaac agcatcagtg cctgcatgta cgcttcagga
    15841 cgcggcgggc agcccgggat atacacatca accgggatga atttatcgac gccctgcaca
    15901 acggaataaa tatcgtacat accaccagag ttggcacagg cacccattga gataacccat
    15961 tttggttcca gcatctggtc atacagacgc tgaataaccg gtgccatttt ggtaaagcag
    16021 gttcctgcaa ccaccatcag gtcagcctga cgcggcgaag cacgcaatac ttctgcgcca
    16081 aaacgcgcca cgtcatgcac cgcggtaaac gaagtcacca tctcaacgta acagcaggaa
    16141 agaccgaagt tatacggcca aattgagttt ttacgacccc agttaaccat gtcattgagc
    16201 ttgcccataa acacgttttt gttaacttct tgctccagag ggtcggttac gatctcctgc
    16261 ttttgcaggg ggtaacggtc gttctcaccg ttgggatcta tgcgggtgag cgtataatcc
    16321 atcttaatgc ctcgcggtta gcgttgacga ttagcgatac tgttcgtttc cgggttcata
    16381 cgctcgcggc gtgaacgcgc gggcgtccag tccagcgcgc caatacgcac cagataaacc
    16441 agacctgcca gtaacacaaa aataaaaatt gcagcttcca caaagcctac ccagccgctt
    16501 tcgcggatag aggttgacca tgcgaacaga tacagcgctt caacgtcgaa gataacgaag
    16561 aacatggcca ccagataaaa cttggcggac aggcgtaagc gggcggagcc gaccgagtcg
    16621 ataccggatt caaacggcac gtttttcgac ctcgcgcgtg cgcgaccgcc taaaaaccaa
    16681 ccgcctacca gcatcaggca acacaggcca atggcaacga taagaaagat agcgaatgcc
    16741 cagtgatgag cgatgacttc agtggatgtt gacatactca ttgcttactc atcaaaagta
    16801 gcgccagatt cactgctctt cacggcagat ggacgccaca tcgattcatg gggaggaata
    16861 aaaaaaacct tacaataact gtagaaattc ttttatacag ctaattgatg tggtctttta
    16921 ctcctttcta taaccttttg tcaactttaa caaaagcttc ttcacattag tttacataat
    16981 atcaacacca ttagcattta atgccctttc accccagatc cttgacgact ccaggataat
    17041 tagatgttgt tgaatcgtgt ccgttgtgaa gcaatggaaa aaatacgggt ctattttgac
    17101 aggaatttgt gtcgattcct ccccccaaaa gagagtattt tcttgatctg tgacacgctt
    17161 ttgttattcc ataacaaaaa cgcagcaaca aatttacgta ttttttaaca tcattgtagc
    17221 aggtgatttt tttcaggcga ttatttgtgc attcgggacg tgaatctctg gtgattgaaa
    17281 aataaacagt tttgtacgtt ctgcactatg cgaggaagga tttttactaa aaaaaagccg
    17341 ctggggttta aaacaccccc agcggctcgt tttttacact attgtctcag gaattatcta
    17401 tcgtccgtcg attactcgat atccctttca atcaacaatg aatcatcccc ttccggagca
    17461 gacattgggc tgtattgcca cggattatgg tagctctcca tcgcctgata gatcacctgg
    17521 gccagctcat tattactgga cgggtcatag cacagcaaat attcggtatc aggtagcggc
    17581 ggtaagccat ctactccact cagcacacgc aggtccgggc tcatcatctc aaccggcctt
    17641 gccgtcacgc caagaccggc tttcactgcc gcacgaacgg ccggaagcgt cgaggcgaca
    17701 taagccagtc gccatggaat atctacttta ttaagcgtcg ccagcaccat atcgcgaaac
    17761 ggacttggat catccagcaa tacaagaggg atcggctcgc ctttttgcaa tacgtattcc
    17821 gctgcgcagt accagtgtgt tggcgaggta cgcaggttca atgccttaaa cgctgaagga
    17881 cgatgggtgg ttaccatcaa atccacttcc tgggattcca gcatttctgc catataggca
    17941 ttacgtttaa cgcggacatc cagcgctaat ttcggataaa ccgaactcac gcgatttaac
    18001 aggaaaggta agatcgtatc ggcagattca tctgaagcac cgatagttaa cacgccctga
    18061 agattactga acattaatga tgagcaggcc tcatcattaa aacgcaggat tttcctggcg
    18121 taaccaagaa gttgaatgcc atgttcagtt aacagtttgt tgcgaccgtg acgagcgaac
    18181 agttctttcc caacgagttg ttccagacgc tgcatttgct gacttacggc ggactgagta
    18241 cgacacacag cggcagctgc ggcagcaaaa gtgttcagat cggcaacagc aacaaatgtt
    18301 ctcagcagat cgaggtcgag gttaattatc ggacgatttg cacttatcat atattatcac
    18361 ttactggcgg ctcatactga gctggttaat gctgtgcaca cacaaacaag caattccatt
    18421 tgtaatgtgc ctccctggca gtttcatccg gaacccggac gaaagtaaaa atgcatatga
    18481 gttgcactaa aaaagcgact cacattgttc cgttataatg cctgaagtag atcacagaat
    18541 atatcttcag ggatcgcata tctattaagt tactcactct tttctattta tgacatgcgc
    18601 gtgtttgtat aaatgtaaat gtgagtcctt gttccactct cgtgcagcat cgctggtcat
    18661 acgcgaacac gtacaaacag caatggtgaa gcggcatcaa gagcagggat ccgtcattta
    18721 tgcgagcatt ttaccccaaa aaccttttat ttataagggt cattgcgaat tatctgatgc
    18781 aaagttatgt tatgttaggc aaagtaatct tctattatta ataagcacat caaaaccttt
    18841 ttgaatatta aataataatt aattagcatc atcctcattc attaattccg cttaacaata
    18901 gtttcacacc ttacccccat tgcgacacct cggaaatcat cacgctgtta aactctccgt
    18961 aatgcggaaa tatcatcatc gcgtttcatt agtgaattct taaatgaggc attttcacac
    19021 aattatctta cagataaaaa aaccagactt gcaattaaga attagaacaa ttcactatat
    19081 aacattgcat gtaaagcata tacacctcat tattttgtca ttattaagtt attaacagca
    19141 caatcgagcc ttcccctctg gcaaaatctt attctgcaga ccttcaaaac accgtcctgg
    19201 gggagtacat tgttctaagc tgacttccac ggcagggagt ggcgataaca gcaaaaaagg
    19261 tcaagattca tgtcccccat tgaaaaatcc agcaaattag agaatgtctg ttatgacatc
    19321 cgtggtccgg tgctgaaaga agcaaaacgc ctggaagaag aaggtaacaa ggtactgaaa
    19381 ctgaacatcg gcaacccagc cccattcggt tttgacgcgc cagatgaaat cctcgttgac
    19441 gtgatacgca acctgcctac cgctcaaggg tattgcgatt ccaaagggct ttactccgcg
    19501 cgtaaagcca tcatgcagca ctaccaggct cgtggcatgc gtgatgttac cgtggaagat
    19561 atttacatcg gcaatggtgt atcggagctt atcgttcagg caatgcaggc attgctgaac
    19621 agcggggacg aaatgttggt tcctgcacca gattacccac tctggaccgc ggcggtttcg
    19681 ctttccagcg gtaaagcggt gcattatctt tgcgatgaat cctctgactg gttcccggac
    19741 ctcgatgata ttcgcgctaa aattacgcct cgtacgcgtg ggattgttat tatcaaccca
    19801 aataacccaa ccggcgcggt atattccaaa gagcttttaa tggagattgt ggagattgca
    19861 cgtcagcata atctcattat cttcgccgat gaaatttatg acaaaattct ctacgacgat
    19921 gctgagcatc actcaattgc gccgctggca cctgacctgc tgaccattac ctttaacgga
    19981 ctgtcgaaaa cgtaccgcgt tgcaggcttc cgtcaggggt ggatggtgtt gaacgggccg
    20041 aaaaaacacg ccaaaggcta catcgaaggt ctggaaatgc tggcttcaat gcgcctgtgt
    20101 gctaacgttc ctgcgcaaca cgccattcag accgcgctgg gtggttatca gagcatcagt
    20161 gaatttatta cccctggcgg tcgtctttat gagcagcgta accgcgcgtg ggaactgatc
    20221 aacgatattc cgggcgtttc ctgcgtgaaa cctcgtggtg cgctgtatat gttcccgaaa
    20281 atcgacgcca aacgctttaa cattcatgac gatcagaaaa tggtgctgga tttcctgttg
    20341 caggaaaaag ttctgttggt gcaagggacg gcattcaact ggccgtggcc ggatcacttc
    20401 cgcattgtca cgctaccgcg tgtcgatgat atcgagctgt ctttgagcaa gttcgcgcgt
    20461 ttcctttctg gttatcatca gctgtaatct taatttcact gccggagatt gcatccggca
    20521 gtgttatccc gccacaatga cctgatgatg tcatcatacg taaggtcact atgaaacaga
    20581 gccatttttt tgcccatctc tcccgcctga aactcattaa ccgctggccg ctaatgcgca
    20641 acgtgcggac ggaaaatgtg tccgaacaca gtttgcaggt agcgatggtc gcccatgcgc
    20701 tggcagctat caaaaatcgg aaatttggcg gtaatgtcaa cgccgaacgt atcgctttac
    20761 tggcgatgta ccacgatgcc tcagaagtgc tcaccggcga tctccctact ccggtgaaat
    20821 acttcaatgc gcaaatcgct caggagtaca aggctattga aaaaatcgct cagcagaaac
    20881 tggtcgatat ggttccggaa gaactgcggg atatctttgc gccgttaatt gacgagcatg
    20941 catatagcga tgaagaaaaa tcgctggtga aacaggcaga tgcactgtgt gcatatctga
    21001 aatgtctgga agaactcgcg gccggaaata atgaattctt gctggcaaaa acgcgactgg
    21061 aagcgacgct tgaagcgcgt cgcagccagg agatggacta cttcatggaa gtatttgttc
    21121 ccagcttcca tctttcgctc gatgagatta gccaggattc accgctgtaa gcagccggag
    21181 tctgcgtcgc atcagtcaat aagcgccgga tgcgacatca ggctcttgtc aaaacggaaa
    21241 cagcatcggg atcatcacca cacaaaccgc catcacgata atggtgaacg gtacccccaa
    21301 cttcacaaag tcactgaagc tgtaatttcc cggacctaaa accagggtgt taacaggtga
    21361 agacaccggt gtcataaagg cggcggatgc tgccatcgcc acgaccatcg caaatggata
    21421 aggcgacact cccatcgttt tggcagcagc cagcgcaatc ggagccatca acaccgccgt
    21481 cgcggtatta gagataaaca gcccaataac cgccgacaag acaaacaaac accccagcat
    21541 catatgtggc ccataaccgc cgccaatatc catcagccct ttcaccgcca gcgcgacacc
    21601 tcccgttttc tgtaatgcca cagcaaacgg catcatccca acgatcaaaa taatgctcgg
    21661 ccagtgaatg gatttatagg cgctttcagc atctatacag cggaatttcc ccatcagcag
    21721 gcaggcgatg atagcggcaa caggattagg aatttcattt gtcagcatta acgccaccat
    21781 tagcaccaga cagaaaatgg catggggtgc ctggctgtgc gcgggcgatg cttcactcac
    21841 ctcttccggt aagttcagcg ctacgaagtc gcggccctgt ttggccagca taccgatcag
    21901 tttccagtta cccacaacca ggatgatatc gcccagcagc agaggctcat ccgccagcga
    21961 accttccagc gccacgccat tgcgcttgag ccccaccaca ttcagtccgt agcgggtacg
    22021 aaaaccaatt tcgcgcaccg atttaccaat cagttctgac tcaggaatta atgaaatctc
    22081 tgccatgccc acatcaaggg cctggtcaga aaaatactcg ccgcgcagta ccatcggctc
    22141 cagcaattgc tcactacaaa attgccggag atcgacatca gccgcagaca tatcaataag
    22201 caaaacgtca cgcgcgcgaa attcagaaac cccattaacg ttcacgataa cgcgacgaaa
    22261 acgccgccag cgttcaacac cgatgacgtt agcgccataa cgctcacgta atttgagatc
    22321 atccagccgt tgacctatca ttggcgatcc ggggcgaata gccagacgtc gcgcacgccc
    22381 ggtcagtcga tattcacgga taagatcgcg aaaggttcga cgcgtccagc cttcgcgctg
    22441 cggggtctgg gtatcccctt tcagcatgaa acgcatcact aacatataca aaatacccag
    22501 caccagcacg accaggccaa taggtgttac gctaaagaaa ctgaagccgt gatagccttc
    22561 acgcagcaat tcactgttga ctaccaggtt cggcggcgtc gccaccagcg tcatcatgcc
    22621 gctaatcagc ccggcaaaac ttaacggcat catcagacgt gacggcgaag tttgcatacg
    22681 catggcaacg cttaacacca cgggaataaa gatagcgaca acgctggttg aactcataaa
    22741 cgcgccaagc cccgcgacgg tcagcatcaa caaaaccaac attttgattt cactgttgcc
    22801 cgccactttg accagccatg ttcccattac ggtggcaaca ccggtacgga ccaaaccatc
    22861 gccaataata aacaaggcgg caatcaggac aacgttagga tcagaaaagc cggaaaatac
    22921 ttcggggacc gtcagcgttc cgcttaatgc aaacgcgaca ataacaaaca aagcgaccgc
    22981 atccatacgc actctgcccg tcgcaaacaa gacgatggca acggccagta atgaaagaac
    23041 ccaaatcaat tcaccgttca caacttatcc ttgttaattg agggggatga cttgattctg
    23101 ccataaaaaa gccccgacga gacggggcta aatcatgatc aggtgtttca ctgaataata
    23161 acatcgccat ttggctgttt ggtcacagta atttgctcca gactgtggag gaccaaatcg
    23221 acctcattca ggcgcggggt atctgccgga gcgttaaccg caatgacatg gcaacccgcc
    23281 gccaggccag aaagcacgcc agcgggagca tcttccacca ccacacactc ctgcggcgca
    23341 agccccagca gctgcgcacc taacagatac gcatcaggtt ctggttttcc gcgcttcact
    23401 cgctcagcgg ttacaaacac ctctggtgcg ggaagccccg ctattttatg gcgcgctcgc
    23461 gctaccggca tggagccaga agtcacaatg gcccacggaa tacctgcttt attcaaatga
    23521 ttgagtaagg cgattgcccc aggaagcgcg gtaatacctt cggtttccgt ggcctcgatt
    23581 tgctccagac gcgtaaactc ggcggcaata tcagcctcgg atttgcccgc cataaaatgg
    23641 cgcaaagagg tgatcgcctg tttaccgtga atgaaagcca gcacctcttc cggcgctaac
    23701 ccatgacgtc tggcccagtt gctccacgcc cgttctaccg caggcaggga atccaccagc
    23761 gttccatcaa gatcaaacag aaaacctttg caccgcacgc gggcctcctc aggcgttgat
    23821 aatttgatta atttcgttgg cgctcaaatg gtactgacgc gggcaggcat gccacacatt
    23881 aagcatgcgc tgatattttt cccacattgg cgtctgggcg ttaaaaccat gagtcccagc
    23941 gtcaaaatgg gtatagcgcc cttccacatt aaccataaag cggacataac cgaggtaacg
    24001 tgcttcagtg gcggcgtcaa agccgaggaa ggtgacacga cgttcatcga tggattgctg
    24061 gtcctgcaaa ttagaccagg aaacatacaa cgcatgatac atctccataa tgtcgatgat
    24121 agtgcggcag gtttcttctt tcagctcgcc aaactcgcga tccaattcac gcatctgtaa
    24181 tccgtaacca cgctcaataa ttgtttgcag gcgacggtaa cgttcagcat ttgccggatc
    24241 gagcatagtc atcatcttgt actgattaga caaaataaga cgttgcgcgt tggtcatttc
    24301 cattgttgac tcctgtatca ctctactacg gtgaaaaaaa agaaggctga gtatgccttc
    24361 ttttatatgc gtaatcaggg gtcaattaca aatcatcaag gaaagtttta tccagttgtt
    24421 tgaaggcgcg cttaagcgtg tcagctaatg cctggtaatc aggcttgcct tcaacgggtg
    24481 ccaacacctg tccagactcc tgcaatttac cgcgaacttc ataaaaccag ttaaggattg
    24541 cagggggtaa tggcgttaca gaacgcttgc ccagccacca caatccctgc atgggtaaac
    24601 ttaaggcgaa cagtgcagtg gcaactgccg gcccaagcag accgcccagg gcaatctgcc
    24661 agcagagagt aaatacggcg atcggcggca taaaacggat cgcataacgc gtcatcttga
    24721 taacgcgatt ttcgacaaag accggggcaa ggcgtttttc cagtggccac gtctttgagt
    24781 aatgctgtcc gcggcgaaac aagctaaaaa aattaacaga acgattatcc ggcgttgaca
    24841 tgcttcacct caacttcaca tataaagatt caaaaatttg tgcaaattca caactcagcg
    24901 ggacaacgtt caaaacattt tgtcttccat acccactatc aggtatcctt tagcagcctg
    24961 aaggcctaag tagtacatat tcattgagtc gtcaaattca tatacattat gccattggct
    25021 gaaaattacg caaaatggca tagactcaag atatttcttc catcatgcaa aaaaaatttg
    25081 cagtgcatga tgttaatcat aaatgtcggt gtcatcatgc gctacgctct atggctccct
    25141 gacgtttttt tagccacgta tcaattatag gtacttccat gtcgagtaag ttagtactgg
    25201 ttctgaactg cggtagttct tcactgaaat ttgccatcat cgatgcagta aatggtgaag
    25261 agtacctttc tggtttagcc gaatgtttcc acctgcccga agcacgtatc aaatggaaaa
    25321 tggacggcaa taaacaggaa gcggctttag gtgcaggcgc cgctcacagc gaagcgctca
    25381 actttatcgt taatactatt ctggcacaaa aaccagaact gtctgcgcag ctgactgcta
    25441 tcggtcaccg tatcgtacac ggcggcgaaa agtataccag ctccgtagtg atcgatgagt
    25501 ctgttattca gggtatcaaa gatgcagctt cttttgcacc gctgcacaac ccggctcacc
    25561 tgatcggtat cgaagaagct ctgaaatctt tcccacagct gaaagacaaa aacgttgctg
    25621 tatttgacac cgcgttccac cagactatgc cggaagagtc ttacctctac gccctgccgt
    25681 acaacctgta caaagagcac ggcatccgtc gttacggcgc gcacggcacc agccacttct
    25741 atgtaaccca ggaagcggca aaaatgctga acaaaccggt agaagaactg aacatcatca
    25801 cctgccacct gggcaacggt ggttccgttt ctgctatccg caacggtaaa tgcgttgaca
    25861 cctctatggg tctgaccccg ctggaaggtc tggtcatggg tacccgttct ggtgatatcg
    25921 atccggcgat catcttccac ctgcacgaca ccctgggcat gagcgttgac gcaatcaaca
    25981 aactgctgac caaagagtct ggcctgctgg gtctgaccga agtgaccagc gactgccgct
    26041 atgttgaaga caactacgcg acgaaagaag acgcgaagcg cgcaatggac gtttactgcc
    26101 accgcctggc gaaatacatc ggtgcctaca ctgcgctgat ggatggtcgt ctggacgctg
    26161 ttgcattcac tggtggtatc ggtgaaaatg ccgcgatggt tcgtgaactg tctctgggca
    26221 aactgggcgt gctgggcttt gaagttgatc atgaacgcaa cctggctgca cgtttcggca
    26281 aatctggttt catcaacaaa gaaggtaccc gtcctgcggt ggttatccca accaacgaag
    26341 aactggttat cgcgcaagac gcgagccgcc tgactgcctg atttcacacc gccagctcag
    26401 ctggcggtgc tgttttgtaa cccgccaaat cggcggtaac gaaagaggat aaaccgtgtc
    26461 ccgtattatt atgctgatcc ctaccggaac cagcgtcggt ctgaccagcg tcagccttgg
    26521 cgtgatccgt gcaatggaac gcaaaggcgt tcgtctgagc gttttcaaac ctatcgctca
    26581 gccgcgtacc ggtggcgatg cgcccgatca gactacgact atcgtgcgtg cgaactcttc
    26641 caccacgacg gccgctgaac cgctgaaaat gagctacgtt gaaggtctgc tttccagcaa
    26701 tcagaaagat gtgctgatgg aagagatcgt cgcaaactac cacgctaaca ccaaagacgc
    26761 tgaagtcgtt ctggttgaag gtctggtccc gacacgtaag caccagtttg cccagtctct
    26821 gaactacgaa atcgctaaaa cgctgaatgc ggaaatcgtc ttcgttatgt ctcagggcac
    26881 tgacaccccg gaacagctga aagagcgtat cgaactgacc cgcaacagct tcggtggtgc
    26941 caaaaacacc aacatcaccg gcgttatcgt taacaaactg aacgcaccgg ttgatgaaca
    27001 gggtcgtact cgcccggatc tgtccgagat tttcgacgac tcttccaaag ctaaagtaaa
    27061 caatgttgat ccggcgaagc tgcaagaatc cagcccgctg ccggttctcg gcgctgtgcc
    27121 gtggagcttt gacctgatcg cgactcgtgc gatcgatatg gctcgccacc tgaatgcgac
    27181 catcatcaac gaaggcgaca tcaatactcg ccgcgttaaa tccgtcactt tctgcgcacg
    27241 cagcattccg cacatgctgg agcacttccg tgccggttct ctgctggtga cttccgcaga
    27301 ccgtcctgac gtgctggtgg ccgcttgcct ggcagccatg aacggcgtag aaatcggtgc
    27361 cctgctgctg actggcggct acgaaatgga cgcgcgcatt tctaaactgt gcgaacgtgc
    27421 tttcgctacc ggcctgccgg tatttatggt gaacaccaac acctggcaga cctctctgag
    27481 cctgcagagc ttcaacctgg aagttccggt tgacgatcac gaacgtatcg agaaagttca
    27541 ggaatacgtt gctaactaca tcaacgctga ctggatcgaa tctctgactg ccacttctga
    27601 gcgcagccgt cgtctgtctc cgccagcgtt ccgttatcag ctgactgaac tggcgcgcaa
    27661 agcgggcaaa cgtatcgttc tgccggaagg tgacgaaccg cgtaccgtta aagcagccgc
    27721 tatctgtgct gaacgtggta tcgcaacttg cgtactgctg ggtaatccgg cagagatcaa
    27781 ccgtgttgca gcgtctcagg gtgtagaact aggtgcaggc attgaaatcg ttgatccaga
    27841 agtggttcgc gaaagctatg ttggtcgtct ggtcgagctg cgtaagaaca aaggcatgac
    27901 cgaaaccgtt gcccgcgaac agctggaaga caacgtggtg ctcggtacgc tgatgctgga
    27961 acaggatgaa gttgatggtc tggtttccgg tgctgttcac actaccgcaa acaccatccg
    28021 tccgccgctg cagctgatca aaactgcacc gggcagctcc ctggtatctt ccgtgttctt
    28081 catgctgctg ccggaacagg tttacgttta cggtgactgt gcgatcaacc cggatccgac
    28141 cgctgaacag ctggcagaaa tcgcgattca gtccgctgat tccgctgcgg ccttcggtat
    28201 cgaaccgcgc gttgctatgc tctcctactc caccggtact tctggtgcag gtagcgacgt
    28261 agaaaaagtt cgcgaagcaa ctcgtctggc gcaggaaaaa cgtcctgacc tgatgatcga
    28321 cggtccgctg cagtacgacg ctgcggtaat ggctgacgtt gcgaaatcca aagcgccgaa
    28381 ctctccggtt gcaggtcgcg ctaccgtgtt catcttcccg gatctgaaca ccggtaacac
    28441 cacctacaaa gcggtacagc gttctgccga cctgatctcc atcgggccga tgctgcaggg
    28501 tatgcgcaaa ccggttaacg acctgtcccg tggcgcactg gttgacgata tcgtctacac
    28561 catcgcgctg actgcgattc agtctgcaca gcagcagtaa tctcgtcatc atccacagct
    28621 ttgcgctgcg gatatctgaa ccggaaataa tcactatttc cggtttttta ttctcttaat
    28681 ttgcattaat cctttctgat tatcttgctt aactgcgctg catcaatgaa ttgcgccatc
    28741 tcactttgca tacttaccac tttgttttgt gcaagggaat atttgcgcta tgtccgcaat
    28801 cactgaatcc aaaccaacaa gaagatgggc aatgcccgat acgttggtga ttatcttttt
    28861 tgttgctatt ttaaccagtc ttgccacctg ggtagttccg gtggggatgt ttgacagtca
    28921 ggaagtgcag tatcaggttg atggtcaaac aaaaacacgc aaagtcgtag atccacactc
    28981 atttcgcatt ctgactaacg aagcaggcga acctgagtat caccgcgtac agctgttcac
    29041 gacgggcgat gaacgcccgg gcctgatgaa cttcccgttt gaagggttaa cctcaggatc
    29101 gaaatacggg acagccgttg gcatcatcat gtttatgctg gtgattggcg gcgcgtttgg
    29161 cattgtgatg cgtacaggaa ccattgataa cggtatcctg gcgcttattc gccatacccg
    29221 cgggaatgaa attctcttta ttcctgcgct gtttattctg ttttcacttg gcggcgcggt
    29281 atttggtatg ggagaagagg ccgtcgcctt tgccattatc atcgcaccgc taatggtccg
    29341 gctgggctat gacagtatta ccaccgtcct ggtgacttat attgccacgc aaatcggttt
    29401 tgccagttcg tggatgaacc cgttttgtgt ggttgttgct caggggattg ccggcgttcc
    29461 ggtgctttct ggctccgggt tgcgcatcgt ggtgtgggtt atcgccactc tgattggcct
    29521 gatctttacc atggtgtacg cctcacgagt gaaaaagaat cctcttctgt cacgcgtgca
    29581 tgagtccgac cgcttctttc gtgaaaagca ggcggatgtt gaacaacgtc cgtttacctt
    29641 tggtgactgg ctggtattga ttgtcctgac agccgtaatg gtctgggtga tttggggcgt
    29701 gatcgttaat gcctggttta ttccagaaat tgccagccag ttcttcacca tgggtctggt
    29761 gattggcatc atcggcgttg ttttccgcct taacggcatg acggttaata ccatggcttc
    29821 atcctttacc gaaggggcgc gaatgatgat cgcccctgcc ctgctggtgg gtttcgccaa
    29881 agggattttg ctgctggttg gtaatggtga agcaggtaat gccagcgtgt taaatactat
    29941 cctcaacagc attgccaatg ccattagcgg tctggacaac gcggtcgcgg cctggtttat
    30001 gttgctcttc caggctgtat ttaatttctt cgtgacgtcc ggttctggtc aggcggcatt
    30061 aactatgccg ttactggcac cgcttggcga tctggtcggt gttaaccgtc aggttaccgt
    30121 gctggcgttc cagtttggtg atggcttcag ccacatcatt tacccaacct cagcttcgtt
    30181 aatggcaacg ctcggtgtgt gccgggtgga cttccgtaac tggctgaagg tgggcgcgac
    30241 cctgcttgga ctgctgttta ttatgtccag cgtcgtagtg atcggcgctc agttgatggg
    30301 ctaccactaa aaatgttaag agccgcaatt gcggctcttt tttcattctg ccgtttcagt
    30361 ctctacggct tcatttttgg cattgcgctt catccacaac gccagcgctt tcagcgagtc
    30421 tggagtgaac tcatcgcagc gtgcggtgat ttcttccggc gtcagccagc aaactccact
    30481 gacttcatct tcctgtaggg cgaagggacc gtgagagacg caactgaaca acgcgcccca
    30541 gacacggcaa tttttatctt cgaaatagaa ctgcccgtgc tcggcaaagg ggacaccggc
    30601 aatgcccaac tcttcttccg cttcgcgacg cgcggattcc agcagttgct catcggcctg
    30661 aactacaccg cctgcggtcg catctaacat gccgggtaaa aagtcttttg tctcggtacg
    30721 acgctggacc agaattttgc ccatgccatc atgcacgacg atgtaagttg cacgatgacg
    30781 cagacactgt gcccgcattt gttcccggct ggcttgtgca atgacttcgt tctcttcatt
    30841 gacaatatcc acccattcag tacttgccaa acgacgctgt tccattatca ggaaaccttc
    30901 tttttctggc gctcttacgg cgcattttga gttgtgggta aattacggat taatcgcgac
    30961 ctgcgcaatg atactttgat cattgagtgc gattacacta agtacgtcat tatccagcat
    31021 gccataaccc gccggattac cgcctttcgg aatactcacc gagccggggt tgaagtggaa
    31081 aatctccccc cgctgttctg ccactggcag atgggtatga ccgtacacca gcacatcgtt
    31141 ctggtttaaa gcaggtagat tttccgggcc aaaaagatga ccatgcgtca aaaacagacg
    31201 ttgtttttcc agtaatacct gttgccacgg cgcggttatc gggaaatgca gcatcatttg
    31261 atccacttcg ctgtcgcagt tgccacgcac agcgataacc ttatgtgcca cttcattaag
    31321 ccgttcagcg actttggctg gcgcgtaacc ctccggtaaa gcattacgcg ggccgtgatt
    31381 caacacgtcg ccaaggatca ccagccactg ggcaccgctt tggacaaaca actccacaac
    31441 acgttccgtc gccggtaacg acccatgaat gtccgatgca aacatcagtt tcatcactca
    31501 ctcctcgtcg aaaaaacgta agctatgata ctggattctg tcgttgctat cagccagatt
    31561 gcttcgccga aagtgcaata aaacgctgga cagacgctcg ctgccactgg aatgtcgcat
    31621 aatccaccag gcgttctggc acctcatcgc catgtagtac caggcggtta atcattagcg
    31681 ccagatcagt atcagcaatg caccattcac caaataaatt cggctgaccc agtgctaaca
    31741 aatgttctgc catcgcgaac agtttctctg cactggcttt tccctcggcc gttagtggcg
    31801 cttttttcgc ccccgcaaag acaacatccg tcggacgctc ttcgcggatg ggcatcagat
    31861 cgctgcgcag ccaggcctga atctgtcgtg cacgcgcacg attttctaaa tcaagcggat
    31921 aaatacgttc ccaggtcggt ggcgcaaatc gatcttccag atactccgca atggcagaag
    31981 attcactcaa ctcaaaatca tcgatttgta ataacggcac acggcgtgtc tgaccgtaac
    32041 cttgccacgt cggctgcaaa tgttcaccgc tgtcgaggtc gatggtcttg atatgaaacg
    32101 acaggccttt ttcctgcaac gccacccagg cggataacac ataaggggag aaaaagtggg
    32161 catctgacca aagcgtgata gcgggtttac tcattatgtc ctcggcaata aagggggctg
    32221 gctatcaaac tatagagcct ttttgtcact gtcacgtgac gaatactcac gtaagtcgcc
    32281 gcgctttcta tacttgtggt gttcagatga ttaaacaacc ggagctgcaa tgatcgatct
    32341 ctatttcgcc ccgacaccca atggtcacaa aattacgctg tttctcgaag aagcagagct
    32401 tgattatcgc ttgattaagg tagacctggg gaaaggcggt cagtttcgtc cggaattttt
    32461 gcgcatttcg cccaacaaca aaattccggc aattgttgat cattctcctg ccgatggcgg
    32521 cgaaccgtta agcctctttg agtctggtgc cattttgttg tatctggctg agaaaacagg
    32581 actctttttg agtcatgaaa cgcgtgagcg cgccgccaca ttacagtggt tattctggca
    32641 ggtaggcgga ctggggccga tgcttgggca aaatcatcat tttaatcacg cagcccccca
    32701 aaccattcct tacgctattg aacgttatca ggttgaaact cagcgtcttt accatgtact
    32761 aaacaagcgg ctggaaaact cgccctggct gggaggcgag aactacagca ttgcggatat
    32821 tgcctgctgg ccgtgggtta atgcctggac tcgcctgcga attgacctcg caatgtatcc
    32881 ggcagtcaag aactggcatg agcggatccg ttcgcgccct gccaccgggc aggcactgct
    32941 aaaagcacaa ctcggtgatg agcgttcgga tagttaacag aaacaggttc tcgtgtatta
    33001 tttcatccta agtaaaacaa cggagaacct gcaatggcac aacctgccgc tattattcgt
    33061 ataaagaacc ttcgtttgcg tacgtttatc ggaattaagg aagaagaaat taacaaccgt
    33121 caggatattg ttatcaatgt gacgatccac taccccgccg ataaagcgcg cactagcgaa
    33181 gatatcaacg atgcgctgaa ttatcgcacc gtaacgaaaa acattattca gcatgtagag
    33241 aataaccgtt tctctttgct ggaaaaatta actcaggatg tgctcgatat cgcacgtgaa
    33301 catcactggg tgacgtatgc tgaagtggag atcgataaac tgcacgcgct gcgctacgcc
    33361 gattcggtat ccatgacctt aagctggcag cgttaatcgc catatcggga ggctgcatga
    33421 acatagtgat caccggaggg acgggattaa ttggtcgcca tttgattccg cgtttgctgg
    33481 agctgggcca tcaaattacg gtagtgacgc gtaacccgca gaaagccagt tccgttctcg
    33541 gccctcgggt gacactatgg caagggcttg ccgatcaaag caacctcaac ggcgttgatg
    33601 cggtaatcaa cctggccgga gaaccgattg ctgataaacg ctggactcac aagcaaaaag
    33661 agcgtctctg ccaaagccgc tggaatatca cgcaaaaact ggtcgatttg attaatgcta
    33721 gcgacacgcc gccgtcggta ctcatttccg gctcggcaac gggctattat ggcgacttag
    33781 gtgaagtggt ggttaccgaa gaggaaccgc cgcataacga atttactcat aaactctgcg
    33841 cccgctggga agagattgcc tgccgggcgc aaagtgacaa aacgcgagtg tgcctgctgc
    33901 gaaccggcgt ggtactggca ccggacggcg gtattctcgg taaaatgctg ccaccgtttc
    33961 gtcttggcct gggcgggccg attggttccg gtcggcagta tctggcctgg attcatattg
    34021 atgatatggt caacggcatt ctctggctgc tggataacga gctgcgcggg ccatttaata
    34081 tggtttcgcc ctacccggta cgcaatgaac aatttgccca tgcgctcggt catgcgctgc
    34141 atcgcccggc tattttgcgc gtccccacaa ccgccattcg gctgttaatg ggcgaatctt
    34201 cagtactggt attaggtgga caacgcgcgc tgcctaaacg gctggaagaa gcgggttttg
    34261 cgtttcgctg gtacgattta gaagaggcgc tggcggatgt cgttcgctga tgtggtttac
    34321 agcaaacatc cgccagttaa ctccctgtgt tacaggatta gtggctttgc gcgataagat
    34381 cgtctggtga aagtcgggtc accatcataa ctaactctct gtctaaacct ctatccagca
    34441 tctcctgagc aatacgcagg gcttcttcgt gtttgccctg cattgcgcct tcttcacgta
    34501 atctgtcagc aatggtcatc agtttctcct tttcttgtgg tgcgcgttct gttatctcac
    34561 caataaatgc acggaaacgc tgggcatccc ctgtttgtaa tacgtaatta aacagggctt
    34621 ttagctgtct gtcattagtg ttccctgtaa ctagcagcga aacaatttgg tcgactaatc
    34681 ccaacagatc gcgctgacga atatgtttct gaattaactc caacagcgcc attttgcggt
    34741 gttgcataat ctcgtcatcc ggcaccacgg taatatccac caacggaaac gctgatgaat
    34801 atattttgcg ggctatagca ggctcggcaa attcatcaag ccagcagagt gaataaggat
    34861 aaggacttct gcaaccatga taaaacagca tcgggatcac caatggaagc tctttatagc
    34921 ccgcatcaag atggttttgc attgccgcaa tggaataacg catcatgcga aaagccatta
    34981 attcttccgg cttactttgg tgctctatca ctacataaat ataacccact ccctcctgcg
    35041 ttttcacaga ccacaagagg tcggaataat attgccgcag gtcttcatca ataaaactgt
    35101 ttggttctag tttaagcgtc gttaaatcac acagtttgcg cagcggcgcg ggaagatgaa
    35161 tattaataaa attccgcgcg gtgtctggat ggcgtaaaaa agatttaaat accgcatcat
    35221 gcggcgtgga agttgtcgat attgtcatgg cgttccgtca cccctcaaat ttaagatgac
    35281 gcgacaataa ccacgattaa cacccaatgc agcaggcagt ttttatcttt ggacagcgaa
    35341 ttcagaagta tttttagcgg actgcaactt ttacatttca gcggaagctg actcgcaaat
    35401 tccccttgaa aatactccgg cagcgttagc caccggagag gaaattattt cagcgatccc
    35461 ttaaggaact gttgcagacg agggctttgc gggttgccaa ataactgctc cggcgcgccc
    35521 tcttcttcta ttttcccctg atggaggaaa atgacgtgag tagaaacatg gcgagcaaag
    35581 cccatttcgt gagtcactac caccatggtt ttcccctctt ctgccagttg ctgcataata
    35641 cgtaacactt cgcctaccag ttcaggatcg agcgccgagg taggttcatc aaacagtaaa
    35701 acttctggtt ccatcgccag cgcccgcgcg atagaaacac gctgttgctg accgccggaa
    35761 agatgcaccg gatatttccc ctgcgcacgt tcgtctatcc cgactttcgc cagatacttc
    35821 accgcccgct cgcgcgcttc ctgcttgctc aggcccaaca cctgaatcgg cgcttccatg
    35881 acgttttcca acaccgtcat atggctccag agattgaagt gctggaatac catcgtcagg
    35941 cgtgtgcgca gtaagcgcag ttgattttta tcggcgactt tgagttgacc gtctttgtcg
    36001 cgcaccagat tgatcgtctg gccattgacc acgatcgatc cttcactcgg tttttcgagg
    36061 aagttaatgc agcgcagaaa ggtacttttc cccgatcccg acgatccgat gatgcttatt
    36121 acatctccgg cattcgcttg cagtgagacc cctttcagca cttcgtgttc gccatagcgt
    36181 ttgtgcaaat cgataacgtt taatttattc tcggacatcg tattctcagt gcgttgaaga
    36241 aggtttcaca tgctgcaacc agcgtttttc cgctctgcga aagaggctga tcaggacata
    36301 agagatgatt aaatagagca ccgcggcaat gccgaaggcg gtaaaaggtt gatacgtggc
    36361 ggcgttaata tcgcgggcta ttttcagcag atccggcaca gtggccgtaa acgccagcgc
    36421 agtagagtgc agcatcagga tcacttcgtt gctgtatgcc ggtaacgcaa tacgcagcgc
    36481 agaaggcaaa ataatgcagc gatacatttt aaaggtcgag aagccatagg ctctggcggc
    36541 ttcaatttcc ccatgcggaa ccgaacggat tgccccagca aaaatctcgg tagtgtaagc
    36601 gcaggtgtta agcgtcagcg ccagcacggt acagttcagg ccactgcgga agaaagcgtt
    36661 aaggaattcg gttcccttaa caatctcaag cgtgtacatg ccggaataga acaccagcaa
    36721 ctgaacatac agcggcgtac cgcgaaaaat ataggtaaat aaccagattg gaaactggat
    36781 gtatttatta ctggagacac gaccaatcgc cagaaacagc gccaggactc cgcctatcac
    36841 taccgacaaa ataagcagcc acagagtgat cgccacacca gtaaagcgat aaccgtcggt
    36901 ccacagcagc ggtttccagt attcatgtaa gatttcgatc acaggtcagc cctcttcaca
    36961 cccacggagt agcggcgctc aaggaacagc agcacaccat tggaaacggt ggtgaaaacc
    37021 aggtaaatca cgccacagac gatggcgaaa tagaacggtt cccaggtact tttgcctgcc
    37081 agctgcgtgg ctttgaccac atcttccagg ccgagtaacg aaaccagtgc ggtagatttg
    37141 aggatcacct gccagttgtt gccaatgcct ggcagggcgt aacgcatcat cgccggaaac
    37201 atgatccgcc gaaacacttg cccgcgagta aaaccgaacg ccgtcgccgc ctctatatgc
    37261 cctttcggca ctgccataaa cgcgccacgg aacgtttcgg taaaataagc accgtagata
    37321 aaaccgagag tgataatacc agcgaccatc ggatcgatat caatctgccc gacgcccatc
    37381 gcctccgtca ccatgtttag cgcaatctgc aaaccataga aaatcagcag catcaacacc
    37441 aagtccggca cgcctcgaat cagcgtggtg tacccttcga aaatcagccc cgaaagccga
    37501 ttttgcgaga gcttaccgcc agcgccaatt aaaccgatga ttacagcgag cactacagag
    37561 ctgatagcca gctccagcgt gacgagcgca ccctgtaaaa taacacctga aaacccatac
    37621 aacatgctgc ctgtcctgtc gtgtgtggtg aattaccgcc tcttgtctcc ctccggttac
    37681 ccgaagggag cgggctttct ttcaccgatg gcgattaacc accataaaca tcaaaatcga
    37741 agtacttttt cgctaatttc tcgtaagtac cgtcagcgcg catttcggca aaggctttgt
    37801 tcagtgcctc gcgcagttcg ttatcttctt tacgcaggcc catgccggtc cctacgccaa
    37861 acagtttttc atctttaaca gacggaccac cgaatttgta atctttaccg acgggttgtt
    37921 tgaggaaacc ttcgctggca gcgacctcat cctggaacgc ggcatcaata cgtccggcag
    37981 tcaggtcaga ataaatgttg tcctgcccct gatacgagac gatttcaatg ccttttggtg
    38041 cccagtgttc attaccgaac gtctcctggg tggtgccctg caatacgcct acccgtttgc
    38101 ctttcagcga ctcgactgtc ggctgaatgt cagaattttt cgccaccacc agacgagaat
    38161 cggcagcgta cagtttgtcg gtgaaggcaa tttcttgctg acgtttttcc gtaatggaaa
    38221 gcgatgacat gatggcgtca atcttcttcg cttttaagga cgggattaac gcatccagcg
    38281 ggttttcgac aaacgtacat tgcgtattga tgcgcttgca caactccttc gccagctcga
    38341 tatcgaaacc aaccagttcg ccttgtgaat tttttgattc aaatggcgca taggtcgggt
    38401 cggtaccgat gcggatgttt tgcggaattg cagcaagtgc cgcagttgcg ctggagaagg
    38461 ccagaaccag agagagcgat agcaccagtt ttttcataat gtcctcaact gacagtcttt
    38521 ttatagggaa tatttacagg tttgttacac ttatcgtgcc ataaaattga ccatcaaggc
    38581 aaaatatccc gccccgttag acgtattatt tttcattaaa aatgcttaag tatgtatttc
    38641 aatgcaccac gatagtgcaa caaacctggc gtgcaccatg atgatcattc catcaggtac
    38701 agcttcccag cgacgtatca gtcaccgtag acattaaagt cgaaatactt tttcgccatc
    38761 ttgtcgtagg tgccgtcctg acgcagctcg ccaagcgcct tattgaaggc agccgtcagt
    38821 tcagcatcat ctttacgtag ccctacaccg gtgccatcac cgaagtattt tttgtctttt
    38881 actgatgagc cagcaaaagc gaaatcttta ccagcaggtt gcttgaggaa tccttcgctg
    38941 gcagcaactt catcttgtaa cgcagcatcc agacgtcctg cagccagatc ggaatagacc
    39001 aaatcctggt tggcataggc caccacatcc acgcctttac tacgccaggt ctcgttagcg
    39061 taagcttcct gggttgatcc ctgcagcaca ccaacatgtt tacctttcag tgaatccagc
    39121 gttggctgaa tcggtgaacc tttggcagca atcaaacgag aatctgcggc gtacagcttg
    39181 tcggagaagg caatctcctg ctgacgttta tcggtaatgg aaagcgacga aataatagcg
    39241 tcgatttttt tcgctttcag tgaggggatc agcgcgtcaa agtcactggc aacccaggta
    39301 catttcacct gcatccgttt gcacatctcg ttaccgagat cgatatcaaa gccaacaaaa
    39361 tcacctttag catctttcga cgagaacagc gcataggtgg tatcggttcc gatacgtacc
    39421 gtctccggta gcgccgcata gctggaagcc gctgcggaga gaccgactaa taaagacaga
    39481 gcgagaatcg acttcttcat acataaccct caagtgaaaa tggctttttt atgttgtgta
    39541 ttgtgttgtg tgtttgcagg ctctttcatg caggtcttat gccatcttga cgacaacagt
    39601 aacattcaac gttaaatatg ttaataagac gttgcattat tgtcctgaag ttgaagatag
    39661 caggtatagc ggttgaatcg cagcgtttcg attgaatggc agaaacaaaa tgtcgaggat
    39721 ttgatcgcgg ttgcaaaatt gccctgaaac agggcaacag cggagttatg cgccctgcca
    39781 gcgggcaaag agatcttcag gaagggttat tgcaaactgg tcaagaacac gattaaccgt
    39841 ctgatttatc acatcatcaa gggattgcgg gcgatgataa aacgccggaa cgggaggcat
    39901 aatcaccgca ccgatttctg ccgcctgagt cattaaacgc agatggccta agtgcaatgg
    39961 tgtttcacgc acgcagagca ccaacgggcg acgctctttc agcaccacat ctgccgcacg
    40021 ggtcagtaag ccatcagtat agctatggac aatgccggac agggttttga ttgaacaggg
    40081 taaaatcacc atccccagcg tctggaaaga accggaagag atgctggcgg aaatatcgcg
    40141 cgcatcgagc gtgacatcag ctaatgcctg cacttcgcgc agggaaaaat ccgtttcgag
    40201 ggataaggtc tggcgcgccg cctggctcat caccagatgc gtttcgatat ctgtgacatc
    40261 gcgcagaacc tgtaataagc gcacgccata aatcgcgccg ctggcaccgc tgatgcctac
    40321 aatgagtcgt ttcataaata gttgccctgt cagacttgca ggcagacttt gcagtatttc
    40381 ggcgggagtt gcaagtcagg gtgccagacc ggcaccctca gcgaaggcat catccttcgt
    40441 tatgcatttc gagattttcc acttcgttct gacgttgcac tgctttggcg tcatcattac
    40501 gtaacgtatc gaggaaatcg aggtagccct gatcaacatc tttggtgacg tagacgccgt
    40561 tgaacaccga gcattcaaac tgctggatat ccggattttc agcgcgaacg gcgtcgatca
    40621 gatcgttcag atcctggaaa atcaacccgt cagcaccgat gatctggcga atttcatcaa
    40681 cttcgcgacc gtgagcgatc agttccgtgg cgctcggcat atcaatacca taaacgttcg
    40741 ggaagcgaat ttccggtgcc gcagaagcga ggtacacttt cttcgctccg gcttcgcgtg
    40801 ccatctcgat aatctgctca gaagtggtgc cacggacgat ggagtcgtcg accagcagga
    40861 cgtttttatc gcggaactcg gcgcggttgg cattcagttt acggcgcacg gacttacgac
    40921 gcagctgctg gcccggcatg ataaaggtgc ggccaacata gcggttttta acgaagccct
    40981 ggcggtacgg tttgcccaga atacgagcaa tttccagcgc gatatcacac gaggtttctg
    41041 ggatcgggat caccacgtcg atatccagat cttcccattc gcgggcaatt ttctcgccca
    41101 gtttcgtgcc catattcaca cgcgcgctgt aaacggaaat tttgtcgata aacgagtccg
    41161 ggcgggcaaa gtatacatac tcaaacaggc acggattgct gaccggattg tcagcacatt
    41221 gacgggtaaa caactgccct tcttcagtga tgtaaatcgc ttcgcccggc gcgacgtcac
    41281 gcaggaaatc aaagcccagc gtatcgagcg ctacgctttc ggaagcgacc atatattctg
    41341 tacggttctc gtcaatatca cgttttccca gtaccagcgg acgaatcccg tttggatcgc
    41401 ggaaagcaac cataccgtgg ccgataatca tcgccacaca ggcatacgcg ccgcggatta
    41461 agcggtttgt ggcagcaatg gcagcgaaaa tattgtcggc ttccagcggg tagtggcgga
    41521 agttgtccag ctcgctggcg aagatattaa gcagaatttc cgagtcggaa gtggtgttga
    41581 tgtggcggcg tttttcttca aacagttttt tacgcaactc gtgagcgttg gtcagattgc
    41641 cgttgtgggc aagcgtaatg ccatacgggg agttaacgta aaacggctgc gcttcagagg
    41701 cgctggagct gccagccgta gggtaacgca catgaccaat gcccatattg ccctgcaaac
    41761 gctgcatatg gcgagcttca aatacatcgc tcaccagccc gttcgcttta cgcaaacgga
    41821 agcagttatt ggcatctatg gtgatgatgc cggcggcatc ctgaccgcga tgctgaagca
    41881 ccgttaaggc atcataaatc gactggttaa ccggcataac accggcgata ccgacaatac
    41941 cgcacatacg tctttttcct cgttaagcca catctcagag cacttacgct ctgggcaaga
    42001 aacttgacga gctttgcaga taatcaaaaa accatctgat gataaaactg aattgcggga
    42061 tcagctgtga tttgctccag tcttcacttt tcgacacccc ggtaaaggag tcgagaaaga
    42121 agagaatggc ggcaacaatc aacacaccgc gcaacgcacc gaaacagacg cccagcaccc
    42181 gatcggtgcc tgacaacccc gttttctcca ccaactggcc tatcacgaag ttcacgatag
    42241 caccaacgat cagggtagcg ataaacagta ccgcgatggc aatcccattt cgaaccagtt
    42301 cgtcttcaaa gcccgtaaac cagactgaca ggtaagtgta gtaatgactg gcaacaaaga
    42361 aagcacaacc ccatgtcacc agcgataacg cttcacgaac aaagccgtgg atcaggctaa
    42421 ccagagagga aaaagcaatc accgcgatta tggcgtaatc aatccagacc atatgtgtcc
    42481 cacgatttta cgccctgtca tcctgttcgg ggcgcattct aacagaaaaa gaaaacgttt
    42541 gcgtagggat ttccttcccg cgcatcaata aaaatggcgc tgaaaaaata ttcaacgcca
    42601 tcgacttttt atgcctttgc ggcatcgtgc aatgcgtgtc ggatgcggcg taaacgcctt
    42661 atccgaccta cggttctaac actgcccaag cctgataaga cgcgccagcg tcgcatcagg
    42721 caagactgta ttaattcggc gtatagccca ttaccacgcc acttaagcca gaaagttgct
    42781 tcaactcacc cagcgaacct ttcagcttat ctttcgaggc atccggccca accagaatac
    42841 gggtaatttt accctgcact ggcgtggatg gcgacgtata aacccgataa ccggcaccgc
    42901 gcagcttacc gacaatctca ttcactttat cggcattttt cagcgcaccc agttgcacaa
    42961 cataggcttt acccgttggc gctgcttttt cttccacgac cggcttcggt tctggcttcg
    43021 gcgcaggtgg tgcttcaacc tttggtttag gcggctccac cggtttcggc ttcggtgggg
    43081 cgaccggtgc aggttccggt tcaaactcgg tgttattggc tgcaatagtg gccggatcga
    43141 gcgacggtgc tgcggcatca cctgcccgca cctcttccgc tgcgccttcc ggcggctgcg
    43201 tcggcaacgc ctgggtggcg gctggcatca tatcaggctc atcacgatcg cccgctttcg
    43261 gcaccagcgg gatagccgcg aactcatcct gataatgttt tttctgcccg tccagcagcc
    43321 ctggaagtac aatcaccccc agcgccacca gcacgatcgt gcccactaac cgattctgaa
    43381 acttacttgc caccgcttct cctcgcgtca atcacttcca tgacatgtgc gaccgtgtgg
    43441 aaagaaccac acaccagcac ggtgtcttcc gctttagcgt ccgccattgc ggcatcccat
    43501 gcctgcgcaa cgctatcaaa tgatttgccg ttacccaaat gctcaagcag ttgttctgcc
    43561 gtggcaccgc gcggcccttc cagtggcgca caataccagt catcaaccac gcttttcaac
    43621 caggccagag ttccggcaat atctttatca tgtagcatac cgataaccgc cagcacgcgc
    43681 ccgtttttcg gtagcgcttt catacgcccg gtgagatatt ccgccgcatg tggattatgc
    43741 gcgacatcaa aaataacgcg tggcgactcg ctcacaatct ggaaacgtcc cggcaaaatt
    43801 gcgctggcaa tcccgtcgcg aatggcattt tcactgactt ccagcccgct ggcacgcagt
    43861 gccgccagcg ccgttgcggc attcggttgc gggacaagcg gcaacggcag attttccagc
    43921 gtgccgtgag catcgctaaa cgcccagtca tgatcggtga cggaatagtt ccactcaacg
    43981 ccccgacgtt gtaacagtgc acctttttcc tgcgccacat cagcaatggt agaaggcatt
    44041 tccggctcac cgacaattgc cggtttttcg ctgcggaaga tgcctgcttt ctcgcgacca
    44101 atactttcgc gatctggacc cagccagtcg gtatgatcca gcgcaatact ggttactacc
    44161 gcgacatcgg catcgacaat attggttgcg tccagacgac cgcccagccc tacttccaga
    44221 atcaccacgt caagttgtgc ctgcttgaac agccacaacg ccgacagcgt accgtactcg
    44281 aaataggtca gggaaatatc accgcgtgcc gattcaatct ccgcaaaaga ggcggtgtgg
    44341 gccgattccg gcaattcctg gccctgcaca cgtacgcgct cggtataacg caccagatga
    44401 ggcgaactgt agacgcccac tttgtaccct gccgccatca gaatcgactc cagcgtacgg
    44461 caggtggtgc ctttgccatt cgtacccgca acggtaaaca caaatggcgc tggtttcagg
    44521 acgccaagac gcgccgcgac ctggctcacg cgctcaaggc cgagatcgat agttttactg
    44581 tgcaggtttt ccagataaga aagccacgaa gccagaggcg acgcggcttg aggagtgcgt
    44641 ttgataatca tggtatccgc tgattcgtta cggtgagaat agcaaaaggg cagagccagt
    44701 ggccctgccc ttatcagtta tcaggcctca ggttcctgat ccggtaccgg gggtaccact
    44761 acgccttcac gcggcgcttc aggattcggc gctggcagat tcatcaactt cgccagaatg
    44821 ctcgccagtt tcaggcgcat ttccggacga cggacgatca tgtcgatcgc gcctttctcg
    44881 atcaggaatt cactgcgctg gaatctaggc ggcagttttt cgcgaacggt ctgttcgata
    44941 acacgcggac cggcaaagcc gattaacgct ttaggttcag cgatgttgag atcgcccagc
    45001 atggcgaagc ttgcagaaac accgcccatc gtcgggtcgg tcagcacgga gatgtacggc
    45061 aagccgcgct cctgcatttt tgccagtgcc gcagaggttt tcgccatctg catcagcgac
    45121 atcagtgctt cctgcatacg tgcgccacca gaggcggaga agcagatcag cgggcagtta
    45181 tcttcctgcg cctgctcaac ggcacgcacg aaacgtgcac ccacaacaga ccccattgaa
    45241 ccgcccataa aggcgaactc gaatgccgca gcgacaaccg gcattccata cagagtgcct
    45301 ttcatcacca ccagcgcatc tttttcgccg gtttctttct gcgcagatgc cagacggtct
    45361 ttatacttct tggagtcacg aaacttcagc tcatctttcg gctcaagctc gctacccagc
    45421 tccacaaggc ttccttcatc taacaggcta tgcaggcgat tacgcgctgt catacgcatg
    45481 tgatggtcac acttcggaca gacctcaaga ttacgttcca gctcagcgcg gtataaaacc
    45541 tgaccgcagc tatcacactt agtccacacc ccttcaggaa tgctcgcctt gcgggtggga
    45601 gtaatgttgc ttttaattcg ttcaatccag ctcattaggg acctttctgt ctgaacctgg
    45661 ttcgatgcca gttttatctt tggggacgca taatgccatt tttgccccca acagaccatg
    45721 aatgttgcac attaaaacat aacagcccga aactttggat aaaaaagtgg tcgaaccgcg
    45781 gagttacttt ttattttgcg gcgcgtgccg cagcgcgttt gtgacggatt atttcgatga
    45841 cgcccggcaa aatagaaacc acaataatcc cgacgatcag cagcttaagg ttatcctgaa
    45901 ccatcgggat tgtaccgaag aaatagcctg cgtaggtaaa aagcagtacc cacaacagtg
    45961 cgccgatcac gttataagca gcgaaatgac ggtacgacat gtggcccatt cccgcaacaa
    46021 acggggcgaa cgttctgacg atcggcacaa aacgggcgag aataatcgtt ttgccgccat
    46081 gtttctcata aaactgatgg gttttgtcga gataactgcg acggaaaatt ttcgaattgg
    46141 gattactgaa taacttttca ccgaacaacc gcccgatagt gtaattgacc gcgtcaccca
    46201 caatcgcggc gatcagcatc agcactacca tcatatggac gttgagatcg ttggtttcca
    46261 gtgatgccaa cgctccggca acaaacagca atgaatcacc cggtaaaaac ggcattacca
    46321 ccagaccggt ttcacagaac aaaattaaga acaaaatggc ataaacccag acgccgtact
    46381 ccgcgactag ctctgccaga tgcacgtcaa tgtgcaggat aaaatcgatg aggaaataaa
    46441 tcaggtccat atttgcctat gccttgtact cgtcatcttt caggctgtaa ctacgttggc
    46501 tacagcctga aatgttccga gcattattct cgttagtccg ccagaaatag cgggcccatt
    46561 ggcggttttg gaagatcata ccggtcaggg taatccaccg cgaccagata cagcccttcc
    46621 gcttttgccg ttgctgccgc cagcgttctg tcctttgccg ccagcaactc tgctatccag
    46681 ctctccggct ggttgtgggc accgacttcc atcaggctgc cgacaatatt cctgaccata
    46741 tgatgtacaa aggcattcgc tttgatatca accaccacat aaggaccgtg acgcgtgaca
    46801 ttaatatgca taacgttgcg ccacggggtt cgggactggc actgcaccgc acggaacgag
    46861 gtgaaatcat tctcgcccag caagcattgc gcagcccgat gcatccgttc agcgtccagc
    46921 ggttcgtaaa aatgggttac ccctttactc agtaccgccg ggcgcagccg atgattgtag
    46981 atgatgtagc gataacggcg agccgtggcg ctaaatcgtg catgaaaatc atcaggtaca
    47041 gctttaaccc aacgcacggc gatgtcacca ggtaaattcg catttacgcc caaggtccac
    47101 gccgcgtctt tgcgctgcgc ggttgtttcg aaatgcacca cttgcccggt accgtgtacc
    47161 cctgcgtcag tacgcccggc gcagaagacg gtgatgggtt cgttcgccac ctgggagagc
    47221 gccttttcca gcttctcctg cacactgcgg acttcattct gccgttgcca gccgtaatac
    47281 ttactgccgt cgtactcaat gcccagcgca attttataaa ctggcagttg ttgctggtcg
    47341 gacattagta cagatactcc tgcaccagtt tctcggcgat ttttactgcc atcagcgcgc
    47401 cgccaaagcg aacgttatcg gccaccgacc agaactggac ttgctccggc ataccgtagt
    47461 cattacgcac gcagccaaca gaaagatgcg gcgtacccga agcatcccct acctgagtcg
    47521 ggaattcgtt ctcttcagag agcacaatat cttcgccctg agcaaacgca tcacgcgctt
    47581 cttctgccgc cagcggacgc agtgcttcaa agttgaccat ctgggcatga ccgtagaata
    47641 ccggtgcctg gacgacgctt gccgaaatca tcagcccttc gtcctgcagg attttgcgta
    47701 cttcgtcaac gatacgacgt tcttcacgca cgctaccttc gctatccggc aacaacggca
    47761 gcatattgaa cgccagttga cgaccgaaga aatcttcttc gtcaatcgga atgccgttga
    47821 gcaatttcgc actctgcccc gctaacgcat cgaccgcttt tttgccctgg gcggaggctg
    47881 aaatcaggct ggtaacactg atacgcgaca aaccgccctg atcgattaac ggtttcagtg
    47941 ccgccagcag ctggctggtc agactgtctg gtacggcgat gacattccgg ttgcggtaat
    48001 cggtcagtac aaacgggttt acttccggca ccaccagcgg tacgtcgggt tcgagagcaa
    48061 acagtccgct gctgtcgatc accaggcaac ctgagttggt cgcttcttca acccaggcag
    48121 cggtagcttc tttgcctgcg acaaaaaatg ccagctgcgc ctgcgtccag tcgaattcag
    48181 cggcatcctg cacggtgatt gttttaccac caaagcgcag ttgttcgcct gcgctttcgt
    48241 tacgtgccag tgcataaatt tccccaaccg ggaactgacg ttcagccagc gtttcaagca
    48301 gggcttcgcc catagcgcca gttgcgccca ggacggcaat gttccagcct tcagacatgg
    48361 tggtttactc cagaaatagc aaagctccct gccaatgtta cagcagagag catgaagaag
    48421 agattaacgt gccggatgat gaacggcgtt aaaacccagt ttacacagca atgatgccgc
    48481 actggcgtca tcacaaatta catacagaga tgaccattca cggcgctcaa gatagttttt
    48541 gcgcagttta tcgaactcac ccggtatccc ggcgacttta cgcagcggtg cgtcatcgcg
    48601 gcgcacatca tacaccaaat gcaccagcct tttcagcgtc ggttgatcga gcggaccatg
    48661 cagcgtaatg cggccaaact caggcgcggg cagcaacgta tccagcgcaa cgtgttgttc
    48721 atgcccaata aacttgctat aggcttcaaa cacttgcgtc gtgccacgcg ctttaccctc
    48781 cagggtatag cctgcgatat gcggcgtgcc gatatccact tttgtcagca attccacatt
    48841 aagttccggc tcgccttccc agacatccag caccacgctt aacttctggc cttcattcag
    48901 gcaggtcagc agcgcggtat tatcgacgac tgcgccacgg caggcgttaa tcagaatcgc
    48961 tccgggcttc aggctacgga tcagtttttc atccgccaga tgtagcgttt tgtacggacc
    49021 atctttaaag agtggcgtat ggaaagtcag aatatccgcg cgctggacta actcatccag
    49081 cgagcggaaa tcaccctcat ccccacggtc ggcgcgaggc ggatcgcaaa gtaaggtttt
    49141 aatccctaac gcttccagtc gcgcctgcaa tcgacgtcca acgttaccaa cgcccacgat
    49201 ccccaccgta cggtcgtgca gtgaaaatcc atcgcgttcg gcaagcatca gcagggagga
    49261 gaaaacatat tccaccaccg caatcgcatt acagccaggt gcagcggaaa aaccaattcc
    49321 cgcctgcttc aaccatgctt catcgacatg gtcggtcccc gcagtggcag ttccaacaaa
    49381 tttaatgggt tttcctgcca gcaaagattc attcactttc gtgaccgaac gcaccatcag
    49441 cgcatccgcg tctgccagtt gagcgacggg gattggacgc ccgggaaccg cgatcacctc
    49501 acccaaacgg ctaaataagt cgcgggcata aggcatattt tcatcaacaa ggattttcac
    49561 gtttgtgtta cctgtatgag acgagagtta accggacaag tatgccataa tctcgcggcc
    49621 aggcatactt gcgaagattt caggtataag gatacgtaat gatacaacct atttccggcc
    49681 ctcctcctgg gcagccacca ggtcagggag ataacctgcc gtctggcgcg ggcaatcagc
    49741 ctttatccag tcagcaacgt acttcgctgg aaagcttaat gacgaaagtg acctcactga
    49801 cgcaacaaca aagagcagaa ctgtgggcgg gtatcaggca cgatattggt ctgtcgggag
    49861 attcaccgct gctttcgcgt cacttccctg ccgctgagca taatctggcg caacgtctgc
    49921 tggccgcgca aaaaagccat tctgcccgcc agcttttagc gcaattaggg gagtatttac
    49981 gtctggggaa tcatcgtcag gcggtcacgg attatatccg tcataacttt ggtcagacgc
    50041 cgctgaatca gctctcaccg gagcaattaa aaaccattct caccctgttg caggaaggga
    50101 agatggttat tccgcaacca cagcagcgcg aggcgaccga ccgtccttta ttaccggcgg
    50161 agcacaatgc gctaaaacag ctggtgacca aacttgcggc ggcaacgggg gaacccagca
    50221 aacagatctg gcaatcgatg ctggaacttt ccggggtgaa agatggcgag ttaattccag
    50281 cgaaactgtt taaccatctg gtgacctggc tacaggcgcg tcagacgcta agccagcaaa
    50341 atacgccgac gctggaatca ctacagatgg cgctaaaaca acctttagat gccagtgaac
    50401 tggcggcgtt atcggcatat atccagcaaa aatatggcct ttctgcgcaa tcatcgcttt
    50461 cttctgccca ggccgaggat attcttaatc agctttatca acggcgggtt aaagggattg
    50521 atccgcgtga tatgcaaccg ctgcttaatc cttttccacc gatgatggac acgttgcaaa
    50581 atatggcaac gcgtcccgcg ctgtggatac tgttagtcgc gattatcctg atgctggtct
    50641 ggctggttcg ttaaccccga cgaaatgaca gtatcgtgac aataataccc agcaccgcag
    50701 agatcgcccc ggcaagaaat accgaagagt aaccaaacgt ggtcgccagc attcccgcca
    50761 gcggcccgga gacgccgagg gcgatatcct gaaacgcggc gtaaccgccc agtgcggtgc
    50821 cgcgaacttg tgaggggacg cgtttaacca cctccacgcc cagcgcagga aagataagcg
    50881 aacatccggc tccggttaac gccgcgcccg ctaatgcgac ccatgcacct ggggcttgcc
    50941 agagcagcaa caagcccacc gtttctacaa gcagagagac aatcgccact ttcacgccgc
    51001 caaaacggtc cggcatccag ccaaacatga cgcgcatcac gacaaatgcg ccgccaaacg
    51061 cggtaagagt aaagcccgcc atcgcccatc ctttgctggc aaagtagagc gaaacgaaag
    51121 tcccgataac cgcaaaacca acgccttgta gtgccagccc taaccctggt ttccagataa
    51181 gcccgacaac gctccacagc gatggacgtt ctcccgccag ggccggtact ttgcgcactg
    51241 tgccgttaca ggcccacgcc agtaagggta ataccattgt ggtgatcgcc agtgcggcaa
    51301 aaccgtaatg gctatgaatc aacaggccaa gcggcgcacc aacagcgagg gcaccgtaaa
    51361 tcgccattcc gttccatgac atcactttgc cagagtgttt tggccctacg atgcctaacc
    51421 cccaggtcag agcgcctgtc agtaactggc tttcaccaaa cccaagaatc aaacgcccga
    51481 tgaccaacag ggcaaatttg aacggtgcgg agacaggcaa aatcgccgcc agcagcaacg
    51541 cgccgccagc cagaccacaa gctaacatcc cctgaagcgc cgaacgtttt gcgccatatt
    51601 gatcggccag tcgcccggcg taaccacgcg tcagcaccgt agccagaaac tgaatcccga
    51661 cggcaatgcc gaccatggta ttgccatagc ccagatcatg atgaacaaac agcgggataa
    51721 ccggcaacgg caaccctacg gtcatgtagg tgagaaaaac cgcaaaagag atgcggaaga
    51781 gcgaaaaact ggcagaagat cgtgtttcgg tttggcttac agcagtcatg cattactcca
    51841 gaatgcagcg caaggcgagg agtatccccg tctcatctct ctggtttcag ggttacggtg
    51901 cgttggcagg atttaacgcg tacgtctttt cagaaggaaa tcgacaaagc gggaagtttg
    51961 cctagaactg gtggcgattg tcaatgatgt gaaaaaggga accatcaggt tcccttttgc
    52021 gttagtgccg gaggccgcat tggcgcgtaa cgtcggatgc gacgctggcg cgtcttatcc
    52081 gacctactcc ggcctactgc gaattaatct tttcagcttg cgcattacca gcgtggcgtt
    52141 ggtgccgccg aagccgaagc tgttagacat aacagtggtc agttcgcgat cggtcgtttc
    52201 ggtcacgatg ttcagacccg cagcctgctc gtccagctct tcaatgttga tgctcggggc
    52261 gataaagccg tgttccagca tcagcagaga gtagatagct tcctgcacac ccgcagcgcc
    52321 cagtgagtga ccggtcatgg ctttggttgc agaaatcgcc gggctcttat cgccgaacac
    52381 ttcacggata gctgccagct ctttcacgtc gccaaccgga gtcgaagtac cgtgagagtt
    52441 caggtaatcg attggggtat caacgccatg catcgccatc tgcatacaac gtactgcgcc
    52501 ttcgccagac ggagcaacca tgtctgcacc atcagaggtt gcgccgtagc caacgatttc
    52561 agcatagatg taagcaccac gcgccagcgc gtgttccagc tcttcaacca ctaccatacc
    52621 gccgccgcca gcgataacga aaccgtcacg gtgagcgtcg taagtacggg aggctttttc
    52681 cggggtgtcg ttgtatttag tagatagcgc acccatcgcg tcgaattcgc aggccatttc
    52741 ccagcacagc tcttcgccgc cgccagcaaa cacgatgtcc tgtttgccca gttggatctg
    52801 ctctactgcg ttaccgatac agtgtgcgga agtcgcacac gcggagctga tggagtagtt
    52861 aacgccatga attttaaacg gcgtggcgag acaggcagaa acgccggatg ccatcgcttt
    52921 agtgaccaca tacgggccaa ccgctttcag gccgcgcggg ccgcgcattg cgtcagcgcc
    52981 gaacacctgg aaacgcgggg aaccgccgcc ggaacctgca atcaggccaa cgcgcgggtt
    53041 attctggtaa gcttccggag agaggcccgc atcagcgatt gcctgctcca tagaaaggaa
    53101 tgcataaatg gatgcgtcgc tcataaagcg cacaactttg cggtcaatga ggccagtggt
    53161 atccagtttt atgttgcccc agacgtggct acgcatgccg gaatccttca gctcctgaga
    53221 gaaagtgatc cctgaacgtc cttcacgcag agatgccagg acttcctgct ggttattacc
    53281 gatgctggaa acaatgccca ggccagtaat cactgcacgt ttcattcaat acctctgtaa
    53341 gtcgcacata gagtaagttt cgaatgcaca atagcgtaca cttgtacgcc gaacaagtcc
    53401 gatcagccat ttaatagaga tatttgcgca gccttacaca catcgctaag atcgagccac
    53461 cgcctgtaag acgagtaact tacgtgaaac actactccat acaacctgcc aacctcgaat
    53521 ttaatgctga gggtacacct gtttcccgag attttgacga tgtctatttt tccaacgata
    53581 acgggctgga agagacgcgt tatgtttttc tgggaggtaa ccaattagag gtacgctttc
    53641 ctgagcatcc acatcctctg tttgtgatag cagagagcgg cttcggcacc ggattaaact
    53701 tcctgacgct atggaaggca tttgatcagt ttcgcgaagc gcatccgcaa gcgcaattac
    53761 aacgcttaca tttcattagt tttgagaaat ttcccctcac ccgtgcggat ttagccttag
    53821 cgcatcaaca ctggccggaa ctggctccgt gggcagaaca acttcaggcg cagtggccaa
    53881 tgcccttgcc cggttgccat cgtttattgc tcgatgaagg ccgcgtgacg ctggatttat
    53941 ggtttggcga tattaacgaa ctgaccagcc aactggacga ttcgctaaat caaaaagtag
    54001 atgcctggtt tctggacggc tttgcgccag ctaaaaaccc ggatatgtgg acgcaaaatc
    54061 tgtttaacgc catggcaagg ttggcgcgtc caggcggcac gctggcgaca tttacgtctg
    54121 ccggttttgt ccgccgcggt ttgcaggaag ccggattcac gatgcaaaaa cgtaagggct
    54181 ttgggcgcaa acgggaaatg ctttgtgggg tgatggaaca gacattacca ctcccctgct
    54241 ccacgccgtg gtttaatcgt acgggcagca gcaaacggga agtagcgatt atcggcggtg
    54301 gtattgccag cgcgttgttg tcgctggcgt tattacggcg cggctggcag gtagcgcttt
    54361 attgcgcgga tgaagccccc gcgctgggtg cttccggcaa tcgccagggg gcgctgtatc
    54421 cgttattaag caaacacgat gaggcgctaa accgcttttt ctctaatgcg tttacttttg
    54481 ctcgtcggtt ttacgaccaa ttacccgtta aatttgatca tgactggtgc ggcgtcacgc
    54541 agttaggctg ggatgagaaa agccagcata aaatcgcaca gatgttgtca atggatttac
    54601 ccgcagaact ggctgtagcc gttgaggcaa atgcggttga acaaattacg ggcgttgcga
    54661 caaattgcag cggcattact tatccgcaag gtggttggct gtgcccagca gaactgaccc
    54721 gtaatgtgct ggaactggcg caacagcagg gtttgcagat ttattatcaa tatcagttac
    54781 agaatttatc ccgtaaggat gactgttggt tgttgaattt tgcaggagat cagcaagcaa
    54841 cacacagcgt agtggtactg gcgaacgggc atcaaatcag ccgtttcagc caaacgtcga
    54901 ctctcccggt gtattcggtt gccgggcagg tcagccatat tccgacaacg ccggaactgg
    54961 cagagctgaa gcaggtgctg tgctatgacg gttatctcac gccacaaaat ccggcgaatc
    55021 aacatcattg tattggtgcc agttatcatc gcggcagcga agatacggcg tacagtgagg
    55081 acgatcagca gcagaatcgc cagcggttga ttgattgttt cccgcaggca cagtgggcaa
    55141 aagaggttga tgtcagtgat aaagaggcgc gctgcggtgt gcgttgtgcc acccgcgatc
    55201 atctgccaat ggtaggcaat gttcccgatt atgaggcaac actcgtggaa tatgcgtcgt
    55261 tggcggagca aaaagatgag gcggtaagcg cgccggtttt tgacgatctc tttatgtttg
    55321 cggctttagg ttctcgcggt ttgtgttctg ccccgctgtg tgccgagatt ctggcggcgc
    55381 agatgagcga cgaaccgatt ccgatggatg ccagtacgct ggcggcgtta aacccgaatc
    55441 ggttatgggt gcggaaattg ttgaagggta aagcggttaa ggcggggtaa tctgctctcg
    55501 cagtgtttgc cggatgcggc gtgaacgcct tatccaacct acgtgtgacg tgtaggcctg
    55561 ataagacgcg ataagcgtcg catcaggcat ggtgctccag acgccgcaac ttacagctgt
    55621 gacgcctgct gaaacaggtt ttcccacata tcggtcacca gcgcctggtc acgcggcgac
    55681 aattcaccgg caccaatggc tttttccaga ctctggctaa cggtcgtatg caccgcctga
    55741 gcggagtggt catcaccact ttccagttct gcgatggcta atgtcaggtg gccacgcaaa
    55801 tacccactgg caaacaactc atcatcactg gcatggtcaa ccatgccgtc gattaatgcc
    55861 agaatgcgtg attcaaactc cgcgatcatc ttctttcctc attgtaaaac gtggcgctga
    55921 cttcagttga gcgcttccgg ccacgggaac tgttccgccg taagttccgg cgtgtgataa
    55981 taattttgta gtgctttaat gaagcgtgcc gggcgttccg gaatgccatt tgtcagataa
    56041 tccatcacct gcgcatgtac ccgccgttga aacaccacac gatccggttc gaaatcccct
    56101 tccagattgt cgcaactgac gttaaacgga tatccagccg ccacgcagaa taaccagtcg
    56161 aacgcctgcg gcttcacttc aacatcttca aactggcttt gcgtctgggc atcgcgtccg
    56221 tccgggcaat accagtagcc gaaatcaacc agctcacggc gcgcttttcc ggcaatacac
    56281 cagtgcgaaa tctcatgaat tgcgctggca taaaagccat gagcaaagac gattcggtta
    56341 tacggtactt ccgcatcagc aggaagatag atcggttcgt cgtcgccttt aatcagacgg
    56401 gtattaaatt catcggcaaa gcagctatta aaaatttcaa tcaactgctc gtagtggtgt
    56461 gtactgttca ttagttcatc cccaaccagt ggaggatctc ctgtccgtgg ctatcgtaaa
    56521 gtaatttggc actcatcacc gccgagacga taacaatcat cgggcggatc agcttttgtc
    56581 ctttgctcaa caccaaccgt gaacccatgc gcgcgccgag gaactgcccc accagcatga
    56641 caaagcccgt cgcccaaatc actttgccgc cgagaataaa cagcagcaga ccgccaatgt
    56701 ttgacgttgc gttgagtaat ttggcgtgag ccgtggcttt ggcgaggttg aatccgcaca
    56761 gcataacgaa ggccagcgcg taaaacgatc cggcagccgg gccaaagaat ccgtcataaa
    56821 aaccgacgca gccaccggca attaacgcga agggtaaacc gtacatccgg cgctggcgat
    56881 cttcttcacc cagctttggc atcagcaaga aatagaggcc gatacaaatc accagaatgg
    56941 gcaaaatctg ccgtaagaca tcagcctgaa cgtactgtac caacagtgcg ccgctcattg
    57001 agccgacaaa ggtcatggca atattgagtt tctgatcgct taaactaacc actttgcggc
    57061 gaataaagta gatagtggcg gaaatagagc cgccgcaggc ttgcagttta ttggttgcca
    57121 gcgcgttagc gggagacatt cccgcagcca tcaatgccgg aatggtgagt aacccaccgc
    57181 caccggcaat cgagtcgata aatcccgcca gcatggcgac aaaaaagaga actcccagca
    57241 acagcggaga aaccataaac aggctattaa acgtttccat tagatcacgt gctcatccag
    57301 tagcgcctgg caggaaggcg gcaacggagg cggtgtcttc ttctcaggct ttgttgttcc
    57361 cggttttgga ggttcaaacc agctttgcag ttctgccccg caaccatcgc ctgatggcgg
    57421 taaaggttga tcttcacact ccagactatc ggcagggcaa cgtaatcgta catgcatatg
    57481 cgcgcgatgc tggaaccagg gtcgcacttt gcgcaaccag tcgcgatcgg tgcccgcatc
    57541 aaggcaaagt tgttgtttaa tcgccggatt aacaaaaatg cgcgtgacgt ctttgtcctg
    57601 ggcggcgagt ttgatcaagc tgaaaatttc tggcttccac agcgtggaga caacgtgttt
    57661 accgtcgcgg gaaactaagt ccagtgcttg cgggcgcaag agctgcgcgg aggtccagcg
    57721 agttttcggc agttgcagaa agatatcgac atccagtccg gtctggtggc tggcatgacc
    57781 gccgttgaaa cgcccaccag cgggcatccc catatcgcca atcagcaccg tacccatgcc
    57841 cagattgctc acctggctac tcagacgctg gataaacatc accagatccg ggtgaccgaa
    57901 atagcgacgc tgatcggtac gcatgacctg ataatgttcg gactgtatcg gcaacgtgtc
    57961 agcgccgaca atacagccat tagaaaaact gcctatcgat tgtgcgctac ccggcacagg
    58021 ttgggttatt ttttgccacg gcgtcgctgc caggctagcg ctactggcaa gcagagccag
    58081 cagcgcaatc gcggttttgt tcatttttta ccagcgtgga atatcagtct tcacatcggc
    58141 attttgcgcc cgctgccgta acaggtgatc cattaaaacg atcgccagca tcgcttcagc
    58201 gatcggcact gcacggatcc cgacacaggg atcgtgacgg cctttggtga tcatctcaac
    58261 ttcttcgcca aagcggttaa tagtgcgccc cggcacggta atgctggagg tcggtttcag
    58321 cgccatatga gcaatgattt gctgcccgct gctgataccg ccgaaaatgc cgcccgcatg
    58381 gttgctctgg aaaccgtctt tggtgatttc gtcgcggttc tggctgccgc gcagcgccac
    58441 tacgtcaaaa ccgtcgccaa tttccacgcc tttcaccgcg ttgatgctca tcagcgcatg
    58501 ggcgatgtcg gcatccaggc ggtcaaagac cggctcgcca agtccggcgg ggacaccact
    58561 ggcaacaacg gtgactttcg cgccgatgga gtcgccctct tttttcagcg cgcgcatcag
    58621 ttcatctaac gcgtcgattt tgtcggggtc cgggcagaag aacggatttt gttcaaccag
    58681 cgaccagtct ttgatttcca gcggaatgtc gcccatctgg gtcaggcagc cgcggatttc
    58741 aataccaaat ttctcggcga gatatttttt ggcaatcgcc cctgccgcca cgcgcatggc
    58801 ggtttcgcgg gcggaagaac gtccaccgcc gcgataatcg cgcagaccgt atttttgttc
    58861 gtaggtgtaa tcagcatggc ctggacggaa gacgtcttta attgcgctgt aatcctgaga
    58921 acgctggtcg gtattttcga tcaacaagcc aatgctggtg ccagtagtaa cgccttcaaa
    58981 aacaccggag agtattttga cctgatccgg ctcgcggcgc tgggtggtat agcgcgatgt
    59041 cccagggcga cgacgatcaa ggtcatgttg caggtccgct tccgtcagcg gaatgcctgg
    59101 cggaacacca tcgacgatgc agccgagcgc cagcccgtgc gattcgccga aggtggttac
    59161 gcgaaagagt tgtccaattg tgtttccagc catcacggct ccgttattgt tgtgtttgcg
    59221 tgtttactta atctttataa atcgcgaaat gttctcgtgc ggcaataagc tgctctttag
    59281 tgagcataaa cacaccatcg ccgccgttat caaactccag ccaggtgaac ggaacatcgg
    59341 gatattgttc cataagatgt accatgctgt tgcccacttc acaaatcaac acgccatcat
    59401 cagcaaggta atctgccgcg ttaccgagaa tgcgacgcgt cagtttcagg ccgtcggtgc
    59461 cagatgccag gcccagttcc ggctcgtgac ggtattcgtt tggcaggtcg gacatatctt
    59521 ccgcgtcgac atacggcggg ttagtgacaa tcagatcgta ttgcactttc ggcaaatcac
    59581 ggaacagatc ggaacgaatc ggaatgacgt tgtggatcag accgtgttct tcgatgttct
    59641 gttcggcaac cgccagcgcg tcgggagaga tatctaccgc gtcgacttcc gcttccggga
    59701 aggcataagc acaggcaatg gcgatgcagc cgctaccggt acacatatct aaaatatgct
    59761 gcggttgctt gctgatgagt ccggcaaatt tattgttgat cagttcacca atcggcgagc
    59821 gcggcaccag cacgcgttca tcgacgtaaa attcatgacc gcagaaccag gctttgttgg
    59881 tcaggtaagc caccggaatg cgctcgttga cgcggcggat cacgcgttca acaatacggt
    59941 gtttttcgct ggaggtcaga cgcgcggtgc gcatatcttc cgggatatcc agcggcaggt
    60001 agagcgatgg taacaccagc tgtacggctt catcccacgg gttatcggtg ccgtgaccgt
    60061 accagatatt tgccgcgctg aagcggctta ccgaccagcg caacatgtcc tgaatggttt
    60121 gcagctcatt tactgcttca tcaacgaaaa ttttatccac gtattcctcc agggcatgat
    60181 cgcaataatt tcggcggcta gtttgccatg aagatgacga taaatcagca ttcacgcgcg
    60241 gtgagtgagg aaaaatacgt ttaaaacgat cgattgtgct acgagtcggg taaactgtag
    60301 gaaaattaga aatagagaca gataaatgaa aaagaaaaca acacttagcg aggaggacca
    60361 ggctctgttt cgccagttga tggcggggac tcgcaagatt aagcaggaca cgattgtcca
    60421 ccgaccgcag cgtaaaaaaa tcagcgaagt gccggtgaaa cgcttgatcc aggagcaggc
    60481 tgatgccagc cattatttct ccgatgagtt tcagccgtta ttaaataccg aaggtccggt
    60541 gaaatatgtt cgcccggatg tcagccattt tgaggcgaag aaactgcgcc gcggcgatta
    60601 ttcaccggaa ttgtttttgg atttacacgg tctgacgcag ctgcaggcca agcaggaact
    60661 gggcgcgttg attgccgcct gtcgccgcga acatgtgttt tgtgcctgcg tgatgcatgg
    60721 gcacgggaag catattttga agcaacaaac accgctgtgg ctggcacaac atccgcatgt
    60781 gatggcattt caccaggcac cgaaagagta cggcggtgat gcggctttgt tggtgttgat
    60841 tgaagtggaa gaatggttgc cgccggagtt gccctgaatg ctggcagcaa atgccggagc
    60901 agcgtaaacg ccttatccgg cctacaaata tgtgataatt caataaattg caattcactt
    60961 cgtaggcctg ataagacgca tcagagcaat actacgaact tcagttcatt caatacatac
    61021 tgaaaacgac acgtcgaatc aatcggttgc ccactactgg tattcacatt caccacaata
    61081 tcgtgtagta ctttatccgt taccggatga cgagttggcg taaccgttac cgttgattta
    61141 tcacaaccgt aagaggtaaa ggttaacaat tctgataccg cgcggcagta tgctcctctg
    61201 ccatcattat cagaaaaatt actgccatca cagagtctgc cgccttccat caggtaggtc
    61261 gaaagcgtgg tggtcactgt tgaccccgtg gactgaattc gtaacgcctt attatggctg
    61321 acataatttt cactccagga gatatcgtat gaagcgaccg gtatcgttgg gcatattgtg
    61381 ttatgcactg taggggccgt atagcttcca aatccgggta aatctgtata ggaataaaag
    61441 gatgcgatcc aagggcaacc ataatcatca gatgcaacgg taattttaaa cgtcatatat
    61501 gcacaacgaa aaattttcgt atctatgcca gggcaatttg agttattaat tgtatcaaag
    61561 tcagtcagta ccaaagttgg agctgaaccc gacccagacc aggaaataac ccgcaacgta
    61621 tcgcctttat acgcaaaaga atcatatttc ccatatgcaa cctcctttgg cgttatcacc
    61681 gtaacattga aaacactgct cgctacagca ccggttacct tgtccatggc ataataaact
    61741 ctcacactgt caaaactcgc actcataagt ttatctccag cataactctt agatgcgccg
    61801 acaacactca gaacgagcag cattaggctc gcgaaaatta tggtccattt tttcatttca
    61861 gaaatactca aaggttgccg tcactgtggc actaaagtct ccggacgtta catctcgccc
    61921 gctctttgcc tgtaaccagg tattaaaatt taggctgtta tttccctgat tcagcgggag
    61981 cgtgacgcct gttgtattgt tgattggcac cgatgtgcct gccgcagttt caataccaat
    62041 cccagcgccg gatgctgatg acgaggagtc aaaggccaga aagccgggta gagcactatc
    62101 ttctgttcct gtcaacgtta ctttgacatt aaacaatgta gaactatgac aatctttcaa
    62161 ttgaaacacc aatggcactc gctctgactg cccaaggttc attaaatcac tggcggcaat
    62221 ggcaggaaaa tgtacttctg ccaactggct accattaatc actggcgtac acgatttact
    62281 gattaaattg ccggtaaagt gcaaattatc tcctgctatt gtggttccac tgcccagcaa
    62341 catcaggcta ctaataaaaa acaacgtacg ctttttcttc attggtaatc tacctgtaac
    62401 gtggcccagg cctcaaagtc cgcctctggc agaatgacac cactcttttt tactggcaca
    62461 gccgttaaca ccggtaaatc ggtttcattc accacaagtg gcgtattgat cgtaaaactc
    62521 tgccctgcct gtttgagctg aatgcctaaa ccagtgatat tgctgcttac tgcagcatta
    62581 tcaaaatcgc ttggtgtgcc actccagctc aaggtcattt ccattaatgg gtcgcgaacc
    62641 gccgtatcgc aggtaatgga atacggcacg gtttgctcat aattgctgcc attaatgtca
    62701 tcaataagca catcctgaaa agttatttca atggtttgag cgttgctaat cgtgcagtta
    62761 ggcggctgca ctaaataacc gtgaaaagag acgtttgaga gtgccatcga gcaaaagccc
    62821 cacagcaaaa taagcgagat ccgtttcatt ggtaatccac cacaattgtg gcactggcgt
    62881 taaattcagc tgccgctaac gttgtaccac tctgtttcac cggcacagct tccagaacgg
    62941 gaactcccga ggaaaaatta aatggcaacc atgagccaga ggttaagtcg agtattgtgt
    63001 gatcactact tttctgaacc cgaataccaa acccaggaat acccgtgctg ataactgttt
    63061 caccgttaat cacgaccgtt gtcgcctgca tctgtagacg caggtcatcc attgccagat
    63121 tgttacaaac aagcgagtag tcaatgggac gtttataatc aaccctattg atcttattaa
    63181 tgaatacatt accaaactca actgccgcac catttaccgt acaaggagga ggggcatcga
    63241 caagcacccg taaattcata cttactgact tactcgctgc aactgcaaag ccgctaccaa
    63301 cacaacatat cagtaaccca gttaccgccc agacagaaca agatcctttc atactggctc
    63361 tcccggcctc agcctttgcg gctctgctct tcatcgacct agcaactgtt accgttgcaa
    63421 gtgaaaaaga gcggcatccg agcgccatag tcgttgacat aggtcagtac cggcacgcta
    63481 cccatcttca cattcagcgg tgcagaggtt ttcggcgaca tcactattag cgagaaaccg
    63541 gctgccggat tgccattttt ctgtgtgctg gcattactga aaatgacgta atacgacgtc
    63601 gggttattga cggtaaattc ctgaccgttg cgggttaaag taatcttata ttgctagggg
    63661 tttttcatgc tgacgttctc cagcgccttc ggtcgccaga acaacttaat acgcgtttgt
    63721 agtgctattt gcagcgtgtt ggctttgttt gattttggcg ggatctcgcg cacgttgaag
    63781 tagaacaggc tttcacgatc ggcaggcaat ttgttgatat ccggcattcc ctgcactttt
    63841 acctgcccgt tcatcattga atcgatgcgt tgcaccggtg gcagaacagt taacggtgaa
    63901 ctgatcttgt tacccttttc atcttcgatc cagctttggg ccagataagg cagtttcgga
    63961 tcgttattgc gcaacgtcac gctgattgat ttgtcgcttt cattaaatat caaacgggta
    64021 cgatctggcg ttacagaagc ctgggcagac aaactggcag cgctaagcag taacgctagc
    64081 gtcgtagcac ccaatttggc agagcaaagt aaatcagaca tggatcattc cttaatcacg
    64141 ttattggtta gctgaaacag aaacaggggg attggtgggt gtcacctgtt gcgtctgctc
    64201 ctgaatgaca ggctttatgt catcaggaac aaccggcgcg acgttgcctt tatgctggca
    64261 tggcagtaac aagccgttaa acagatcggc aggcagcggg tccggcaggt tgatatcgca
    64321 atgcgcaaca ccactccaga acacactcat gtgttcgcca ggtttcaccc cagccagata
    64381 aacattgccg tcatcatcga caaggcccac tgtctgctcg ttatcatttt ttacttctgc
    64441 gccaaacgga ggatggctgc cgtcttgtaa acgcagcacc gccatcgctt tttgaccact
    64501 gatgacggca aatttgcggt agccaatcgc cccttcagtt agcgtggctt gtaccaccga
    64561 ctgggttgct tcggcgtttt ccggcagttt gttgaggtcg atatacgcct gattgcggta
    64621 atagttattg acgtcagaaa cgacggcttt accaaacata ttggtataaa cagcagcacc
    64681 gttaccttct accggaacat cggcaacgcc atcggcatca atcaacaagc gtgtcccgcc
    64741 catattctgg gtacggtgaa gtgcgccacc gtgggcagta agcgttgcgc cgccctgtaa
    64801 cgacaagccc gcagaggtgt attgcccttc atggtagttc gcactgaggt caacctgcgc
    64861 cagcgaacca tcatggctgt aatagccgtc aacgctggtg tgtttgtcac tggtgccgac
    64921 gttcaactga tagtgagtcg cgtcatcgac acggctgaaa taaccgacct gactgctgtc
    64981 cgtcccactg ccatagtacc gttatagcta acggtactgt tgtcgcccca cggcatactg
    65041 agcgaaatgt acatgccttt gtcggcctgg ttgtcatact cgtaacggta gccagtcatc
    65101 gagatgctga cattacgaat actgcccata ttgaaatagt gcgagagcat gatgttgtag
    65161 tttgtctgct cctcgcgatc ccagtaggta tggcgggtat agttgagata aaccgaaaca
    65221 cccgcatcgc ggaagttctg gttataagtg gcggtgtaca tctctttgtc gttgcccgtg
    65281 cgtaccattc cgctgtcact ggcatccaga tactcgctca tggtcataaa gttctcttcc
    65341 gagaagcgat atccagcaaa agtaacgcgg ctgttgagct ggtcaaagtc tttggaataa
    65401 ctcacacgga aggagttacc gtccagcgaa cctttgccat aagcggtgtc tttatccagt
    65461 ttggtgtgcg agtgagtaac atcaaacgcg accgcgccga atgtagacaa atcgcgaccg
    65521 acaccaagcg ccgcagactg atagttttca tctcccagtg cgccgccata taacgaccag
    65581 ccgttagcga tcccccagga agcttcagca ccagaaaaaa attcaccctc gacatggtgt
    65641 ccccactctt gcggacggcc catcatgatc ttatagcgaa cctgacctgg acgagtgagg
    65701 tatggcatcg aggcggtgct gatgtcatat tcctgcacct ggccgttctg ttcttcaatg
    65761 cgaatatgca acgtaccgga gacagaatca ccaagatcct gaatacgaaa cggtccagcc
    65821 ggaacctgcg tttcgtaaat cacacgcccc atctggctga cggtcacttt tgctgtggtg
    65881 tgtgccacgc cggaaatgtc tggcgcgtag ccgcgcagat tgggaggcaa catttgatcg
    65941 tcagtactga cactgccacc aacatagtta aaaccatcaa aaatatcgga tctgaggtaa
    66001 tcctcgccca gcgccagttt ggcttttaat gatggtaacg cccgcctggc atagtagcga
    66061 ctccactccc attttttttg agtttcatcg ccgctgaatt catcgtcatc attactgcga
    66121 gtatgttgat agttagtctg ccagtcagca cgcatacgcc acggccctag gttaacccca
    66181 accgtcccgt tgccgctgat ctcgttacta tcatcaccgc cattttcttc gtgccgggtt
    66241 tgtgcgttga tgctgtagtc cgcgacgatc ccggagatgc cgtcatccca acgtgaaggc
    66301 ggatcccagt cgggataagt atattcgagg taagcctgtg gcagtgaaat gactaatgcg
    66361 gactggctta aatcagcctt aatttccatg ccttccagtt gaccggattt caggcattta
    66421 gcatcgtggc tccattgcag atttttcgcc acgtcttctt ttaaaccaaa ctgcgctacc
    66481 agttccggtg tcagacaagc atagcttttg ctcgcgtcat cttcaccagc ataccagtaa
    66541 atatcgtact cttccgccaa tggctgttta tttagttgaa cctgtaaatt gtattttccg
    66601 ggctcaacat atccctagct ggaaaaacgc ttcagatcaa tttttgtgtc gccttttaat
    66661 tccagaaaac gggaatcaaa ctgaatgtcg tcttcagccc agacactgct ataactccct
    66721 gacattgcca gcgcaatgca ccacggaaga atccgtaatc gaaaaagaga atggtcaggc
    66781 atacacgttt ctatccgttt ttattcttgt tcggtcaaaa tacatttaaa tcccccatag
    66841 cagtgtcgct atgggggtat gccttacagg taagtaatct ggaaggtggt atttgcttca
    66901 aaattaccca gatctggcgc atcagctgcg ccgaccagcc aggcgttaaa gttcagtgtc
    66961 tgcttcgctt tgcctttgtt ggtagaagtg tctttcacta ttttctggtc aatacccatg
    67021 ccgcttttgt aagaggtacc cagagagtca ccgatcgcca ggctaacatt gttaaatgcc
    67081 gcaccggtat cggtattgaa aatggtgtaa taattgccgc tatttgcaga agaaacggta
    67141 ccagtaaagg tagtggtcat tgtttcctga gtatcaaata cgcaatcttg cagacgaatc
    67201 tggaaacttt tcggcgtggt ggtgccgtta tttttcagtt tttcggcacc gatatcaccc
    67261 atatcaactt ccagtttgtg atcgtctgga acgatagaac acactgagtc ttcaatggtg
    67321 ccgtaaaaac gtgcagtacc gttgttaccc gcagcgattg ttgccgtaga ggtgaacacg
    67381 cccatcacca ttgccgcagc aatagctgtt ttaacaaact tactcattat ttgtccttaa
    67441 actataaata attaaaaata taaacattgc aatacattga ttcagtcaat agccaatgtt
    67501 tatacccagc cattgaatac ggcatgcgac ccaaaaagaa caggtcgtag aaaattgtat
    67561 tcattggtgt ggggccgcat agtgatgttt ccacacccct gatccaattt aattttacta
    67621 aagagtaaat gtagatttaa ttaatatatt gacagaagaa aggttatctt aaggacatcc
    67681 ttaaaaaata cggaataagt atttagaaaa aatagaaagt atatcaataa tgaatatcaa
    67741 cagcttaaaa aacaaacaat gaacatataa tgcgattggc atttatccac ttttgacaat
    67801 ataatatata aacactatag ccagaattta atcaagtttc aattacatcc tgcctgatta
    67861 ttcaactcta ttttctctat tatctgtatt aataaatgac aatgagtgtc aaacagatat
    67921 tattcatttc aacatgtaac tttgggaaaa atcaagatac gcgcagaaga ataatttccg
    67981 aatgaatgta gaaagtgttt tatttataga taaggagagt cttcgatagt agtgaggttg
    68041 acagatatcc gctgcctaat gagcaattat gcccaatgaa tctacctcat tgggcataat
    68101 ttggaacaca tcagatagct tttgccatct tcagattaca cgggctcatc tgccagttga
    68161 atgtaccgtt tccgctttca tcaagcgtta cgctggcaat ggccgaggtg gtaaacatag
    68221 gcggcgtttc gcccggacat aactcggcaa ccagataccc gactaacggt aagtgagaga
    68281 tcaccagtac cgaagcaaca ccttcattgg tcaacgcctg caaataggca ctgaccagac
    68341 caacatcgcc gcagggcgtt aactccggca gaacttctgc actggaaggc aggttcagac
    68401 aatcccctac ttcttccagt gtttgctcgg ctcgcaggaa cgggctcacc agaacgcgtt
    68461 cgatttccac tttttgacct ttcagccagt tcgccatcag gcgagattcg tcacaaccat
    68521 tagtggtcag aggacgaacc gaatcactgg cggaatcgag ggctgcgtcg ccgtgacgca
    68581 tgataaaaac ttgcatattg caccgctttt gttaaccaga ttcgcccgtt ttctttcatc
    68641 tgaaaccaga gaaagaaaac ggtggccggc attgtgcctt atccattcac cgaatgaaac
    68701 gctgtttttt acctcaatgg cgtaagtata gtcaatatgc gtttacattt tgaccaacac
    68761 tccgccattc agcgcggatt catatagctt tgaccttctt attgcaggtc agttgcagtt
    68821 gttttccaaa aactttcccc acgcgcgccc atctcgacca aacgctcgca aggggtaaaa
    68881 cgggaaccat actgcgtggc cagtcgttgc attattgcaa ccacttcgcc cgcgccgaga
    68941 gaatcgatat agcggaacgg tccaccgaga aatggcggaa aaccaatgcc aaataccgca
    69001 ccaatatccc cgtcacgcac gctacggata acctgctcat caacacaacg tactgcttca
    69061 ttcagcatca acatcacaca ccgttcagca acctgcggtg cggagagtca cccctgccct
    69121 tgtgcgccaa tgagcgggta aatggcagga tcgacctgtt ttttgctttt acgccctttc
    69181 tgaccataaa gatagaaacc ccgaccattt tttctgcctt tgcgatcgtc gttcaaaatt
    69241 gaagaaacaa catttgcagg cgcgctaaaa cgttctccat aagcggcttc cagtacagga
    69301 ataattttag tcccggtgtc gattcctacc tcatccaaaa gttggattgg gcctaccgga
    69361 aaaccaaatt tcactagcgc ggcatcaatg tgctctaccc gttcaccttc ggtcagcatg
    69421 cggatagctt cattaatgta aggcgctaag atgcgattga cgtaaaaccc ggctttgtca
    69481 cgcacgacaa tgggcgtttt gccctgtttt ttcgccagtt ttaccgtagt ggcgatggtt
    69541 tgcgccgatg tacccgcatg aggaataatc tctaccagcg gcattttttc caccggactg
    69601 aagaaatgca ggccaataac ttgctcaggt cgcgcggcgt gagcggcgat atcaccaatc
    69661 ggtaaagatg acgtgttcga agcaaaaatg gtatgagcgg cgcaattttg ctccacttcc
    69721 gccaccatct gttgttttaa ttcgagattt tcaaacaccg cttcaataat cagatcgcga
    69781 tgggcaaagc cgcgatagtc cgtcgttccg gagattaatg ccagctgttt gtcacgttcg
    69841 ctggctttga gatgacgacg gcgaactttg ccctccagct gatcccaact gtacttcagc
    69901 gcatgattta tgccctgcgg gttgatatct ttaattctga ccggaagccc cgctttacaa
    69961 gcagtgacat aagcaatacc gccgcccatc aagccaccac ctaaaattcc cacgctgttt
    70021 aatgccgcag gcggtgcatc actaccggga tctttcttca cgtccgtact ggcaaaaaag
    70081 atactacgca aagcttgcga ttgcggcgtc atcgccagtt cgccaaaggc gcgagcttca
    70141 gcgtcatagc cgctgctggt gccctgcgct aatcccgttt caacaacctc caggatgcgt
    70201 tctgtcgccg gataattgcc ttgagttttg tgttctgttt tcttgccgac cattttgaac
    70261 agcagcgcac gacctaacgg ccccgccaga atacgctcgc gtacaggtag agggcgggaa
    70321 gatgggcgat ccttcttcgc cagctcaaca gcggcttcca gcaaaatgga gtgcggaaca
    70381 acgtcatcca ccagccccag ctttaatgcc tgtttcgccc gaagttgttt tccggtgaga
    70441 atcatctcta atgctgtact gacgcctatc agacgcggta aacgctgggt gccgcctgaa
    70501 ccgggtaaca atccaagttg tacttcagac aaaccgagca ccgttttagg atcgtcagta
    70561 caaacgcgac cgtggcacgc cagcgccagc tccagcccac cgcccaggca agcgccatga
    70621 atagccgcga taaccggaat gggcaaagca tgaatctccg ccatcaactg ttggccctgc
    70681 cgcgccagtg cttccgcttc ttgcgccgtt ttgcagttgc caatcatgtt gatgtctgct
    70741 ccggcgataa aattgtccgg tttcgcggag ataaacacca cgccgcgcaa ctctttgttt
    70801 tcacggagtt gcttaataat ggcgcgcacc tgcgaggcaa attccgcctt cagggtattc
    70861 attttctcat ccggtacgtc gatggtgatg atagcaatgt tgtccagacg aacattaaga
    70921 gtaaacgctg atgccatttc cattattccg cctccagaac cattgccgcg ccaagcccac
    70981 cggcagcaca ggcggtaact aaaccaaatc caccgccgcg acggcgaagt tcatgcaatg
    71041 tctgggtaat catccgcgcg ccggtcgccg cgaagggatg tccgtaagca atcgaaccgc
    71101 caagcacgtt aaatttgcta tcgtccactt cgccagtggc atgtgcacgc cccagtactt
    71161 cacgagcaaa acgttcacta cccagcaact gaatattcgc cagtgtctga gctgcaaagg
    71221 cttcgtgcat atcgatcaat gtcagttcgc tcatcgtcaa accggcacgt tccagcgcca
    71281 gcggtgttga ccaggctgga ccgagcaaca tgtcctgcca gacatcaatc gcagtaaatg
    71341 cgtagctgcg cagatacccc agcggcacca gccctaattc tttcgcccgg gattcagtca
    71401 tcaggatcac cgctgccgcg ccatcggtca gcggcgtact gtttgccgcc gttaccgttc
    71461 cgtgtttgcg atcaaacgcc gggcgcagct ttgcgtaatc ggcaagcgag gaattaccgc
    71521 gaatattgtt gtcttcgaca agcggttgtt tataaggagg gataaaggca gtcatcacct
    71581 cttctttgag ttttccgtct gaccatgcct gagcggcacg ctgatgcgaa cggtgcgcta
    71641 atgcatcttg ctgttctcgg gtgatgccgt aggttttcgc catttgttcg gcggtgtcgc
    71701 ccatccgcaa gccagtagaa tattctgcta ccgcaggcgg tacgggcatt aagtcgcgca
    71761 aacgcaggcg agagaagagt ttcaggcgct ggctcatggt tcgagctttg ttgacatcaa
    71821 ccagcacgtg cgccagtttt ttactgacgc caattggcaa tacggaagag gaatctgccc
    71881 caccggcaat ccccgctcgg atagttcccg ccatcaggct ttctgcgacg tttgcaaccg
    71941 cctggaaact ggtagcgcaa gcgcggctga cgctgtaagc atcggtatgc acattcattc
    72001 cggtaccgag aacaatttca cgcgcaatgt tgggggcttc aggcatttgt acgacctgac
    72061 caaagaccag ctgttcaatc acttcagcgg ggatctcgct gcgtgccagc agttcgccta
    72121 ccaccatctt ccctaaatca accgtgggaa tgccatgaaa agccgtcgcc tggcgggcaa
    72181 aaggcgtacg taaaccgcta acaatggcga tacgatcgcc ctggcgggta accagtggta
    72241 aaacctgacc cataacactc ccctgtaaaa aaataaataa agtggtctga cctgatcata
    72301 gtcttaacca tttttttaca tttagccaag cggagaaaaa ggaaagtggg agctatgaca
    72361 cagtgaagac agaagaaaga aaagaaaacg cccctgccag caactgacag gggcgtacgt
    72421 acccgagagg aattaacgca gacccagctg gaagatcagc atttcagctt cgcaggcaaa
    72481 agtgaaatcg atatccaggc gcacaccgtc agactcttca gtgaaagtcg gggagatttt
    72541 gcacggttcg gattccacgc tacgggattt ttcagtcagc gccgccagcg tttgttctgc
    72601 ttctgcgcgg tttgcaaaca cgcggctgta agacgcggtg cagtcggagt tgtccataat
    72661 ggtgccaaca tccatacagc agcaaaccgg ggtttcatca gcactacatt tactcatcgt
    72721 tgatttcctc tgtatgtgca cccaaggtgc cagataaacg ttgtggatat tttacgcttc
    72781 cggaaagtgc tgctccagtt gttaattctg caaaatcgga taagtgaccg aaatcacact
    72841 taaaaatgat ctaaaacaaa attcacccga atccgcgagt gcgcaacctc caaattttgc
    72901 cagctggatc gcgtttctta gatcatattt gaaaaaagat ggaaacatac ttgcaacatt
    72961 ctagctggtc cgacctatac tctcgccact ggtctgattt ctaagatgta cctcagaccc
    73021 tacacttcgc gctcctgtta cagcacgtaa catagtttgt ataaaaataa atcattgagg
    73081 ttatggtcat gagccagaaa accctgttta caaagtctgc tctcgcagtc gcagtggcac
    73141 ttatctccac ccaggcctgg tcggcaggct ttcagttaaa cgaattttct tcctctggcc
    73201 tgggccgggc ttattcaggg gaaggcgcaa ttgccgatga tgcaggtaac gttagccgta
    73261 accccgcatt gattaccatg tttgaccgcc cgacattttc tgcgggtgcg gtttatattg
    73321 acccggatgt gaatatcagc ggaacgtctc cgtctggtcg tagcctgaaa gccgataaca
    73381 tcgcgcctac ggcatgggtt ccgaacatgc actttgttgc accgattaac gaccaatttg
    73441 gttggggcgc ttctattacc tctaactatg gtctggcaac agagtttaat gatacttatg
    73501 caggcggctc tgtcgggggt acaactgacc ttgaaaccat gaacctgaac ttaagcggtg
    73561 cgtatcgctt aaataatgca tggagctttg gtcttggttt caacgccatc tacgctcgcg
    73621 cgaaaattga acgtttcgca ggcgatctgg ggcagttggt tgctggtcag attatgcaat
    73681 ctcctgccgg gcaaactcct caagggcaag cattggcagc taccgccaac ggtatcgaca
    73741 gtaataccaa aatcgctcat ctgaacggta accagtgggg ctttggctgg aacgccggaa
    73801 tcctgtatga actggataaa aataaccgct atgcactgac ctaccgttct gaagtgaaaa
    73861 ttgacttcaa aggtaactac agcagcgatc ttaatcgtgc gtttaataac tacggtttgc
    73921 caattcctac cgcgacaggt ggcgcaacgc aatcgggtta tctgacgctg aacctgcctg
    73981 aaatgtggga agtgtcaggt tataaccgtg ttgatccaca gtgggcgatt cactatagcc
    74041 tggcttacac cagctggagt cagttccagc agctgaaagc gacctcaacc agtggcgaca
    74101 cgctgttcca gaaacatgaa ggctttaaag atgcttaccg catcgcgttg ggtaccactt
    74161 attactacga tgataactgg accttccgta ccggtatcgc ctttgatgac agcccagttc
    74221 cggctcagaa tcgttctatc tccattccgg accaggaccg tttctggctg agtgcaggta
    74281 cgacttacgc atttaataaa gatgcttcag tcgacgttgg tgtttcttat atgcacggtc
    74341 agagcgtgaa aattaacgaa ggcccatacc agttcgagtc tgaaggtaaa gcctggctgt
    74401 tcggtactaa ctttaactac gcgttctgat aacgcgttcg cctagataaa gtcacctgct
    74461 atgcaggtga ctttaactcc cccacttcac cgaaagtagt ttcctcattg cttgcctcct
    74521 gagttttgca aacagcctga tggcagcttg ccctttcaaa tcagtaagta gtatccacag
    74581 acaaacctga agggaaatgc atattttcaa gcgttctgct cgtccttctc aaagagttta
    74641 cttttctgca tttccaggat actcaacccc cctggctatt gtgcgcttat acactcaaat
    74701 taaagatagg ttctaaataa atgagcgttt tttgatagtc tatttcatta ggtaatatat
    74761 atttgtaaca aatcaatcaa catggaataa aatcatgcta ccatctattt caatcaacaa
    74821 taccagcgca gcttacccag aatccatcaa tgaaaataac aatgatgaaa ttaatggatt
    74881 agtacaagag ttcaaaaacc atttttaatg gtaaggaagg aataagcacc tgtattaaac
    74941 atctacttga gcttataaaa aacgccatac gagtaaacga cgatccttat agatctaata
    75001 ttaataattc ctcagttact tatattgata ttggctccaa tgatacagac catattacta
    75061 ttggtatcga caaccaagaa ccaatagaat tacctgcgaa ctataaagac aaagaactcg
    75121 tccgtactat cattaatgac aacatagttg agaagactca tgatatcaat aacaaggaaa
    75181 tgatcttcag cgcattaaaa gaaatatatg atggagatcc tggttttatc ttcgataaga
    75241 tatcacacaa actcagacat acggtaacgg aatttgatga gagcgggaaa agcgaaccaa
    75301 cggacttatt tacctggtac ggtaaagata aaaaaggcga ctctctcgct attgtaatta
    75361 aaaataaaaa cggaaatgat tacttatttc tcggttacta cgatcaggac gactaccaca
    75421 ttcaaagagg aattcgtatt aatggtgata gtctcaccca atattgtagt gaaaacgcca
    75481 ggagtgcttc agcgtggttt gaaagcagta aagctatcat ggcagaatca tttgcaactg
    75541 gttccgatca tcaggttgta aacgagctca acggggaaag actgagagaa cctaacgaag
    75601 tttttaaacg tttaggtcga gcaataagat ataattttca agtggacgat gcaaaattta
    75661 gacgcgataa tgtaaaagaa ataatttcta ctttattcgc taacaaagtt gacgttgacc
    75721 atcctgaaaa taaatacaaa gactttaaga atctcgaaga taaagttgaa aaaagacttc
    75781 aaaatcgcca gacgaaatat caaaatgaaa ttaatcaact atctgcacta ggtgttaatt
    75841 ttgatgatat ttaaaattat ttttccagtt ttttgatttt tcaacgcctg aatatcggaa
    75901 atagtcagtg acaaacatca gtatttttac atcattacgt ccgggtcatt gtcggtttat
    75961 ctccttttac ttgtggtaat tgagggataa caacaaaaaa ggtgagtttt gcgactcacc
    76021 ttttttattt gtttcttatt cagaatcgat atcttttaaa tcatcctgaa tcgcttgcgc
    76081 gttcggattt tcctgcggtt tgagttcgcc gccattagcg atgaaatcat gacgctggaa
    76141 gtacgcttcg cgcaccataa tataaggatc ggacgactga cgcagcagac catcggaatc
    76201 cagcagctga gcgcgggttt cgatcccttc aagcgtccat ttacccacag acatcggcca
    76261 ggtcagccag gaaagaaccg ggtaaagagc atccgccata tcaccaccgt catcacgcag
    76321 cgtgaagcta ccgtagaacg gtaactgaac gtaaggccca taacccacgc cataatgacc
    76381 aagcgtacta ccgaagcggt gaggttcagt ccgttgcagt ttcgggttcg ccatccctgc
    76441 aacatcaata aagccgccca tccccaaaat ggtgttcagg aaaaagcggg taaagtggac
    76501 catcccctga taagggtcgc cctgcaagaa gtagttaacc atcaccgcag gttcttcaag
    76561 gttgccagta aagttgctca aaccattacg cgccggttgc ggaacataat cacgccaggc
    76621 gacagcgacc ggtcgaacaa tatacgggtc taatacgttg aagttgaagt tgtacatggt
    76681 gcggttgaac ccttctaacg ggtcagaacg cccttgctga tctgtaccgg aactcgcaca
    76741 ccccaccaga agcgtagttc ccagagcaag cgccgacagg cgaagcttca taaatgtctc
    76801 cctgtttttt tatggcttat gcagtttgcc atccatgacg gaacgatacc gtatccgcct
    76861 gtttaggtgt gggcgattgt aacagcacgt caactgatgt ccagacgccc tgatttgctg
    76921 atttgatcat agcctggtaa tcgccgccct gtaggctact tgattctata gaaacagaaa
    76981 aaggcaaacg cagccttttc tacattttca gagtaactcc cgccagttgc gagcaaaaaa
    77041 gccgctacgc tttagctata cgtgctaatt caagagaaga gaccatggac aacgacaaaa
    77101 ttgatcaaca cagcgacgaa attgaagttg agagcgaaga aaaagagcgc ggcaaaaaaa
    77161 tagaaataga tgaagaccga ctcccctccc gagcgatggc aattcatgag catatccgcc
    77221 aggatggtga aaaagagcta gaacgcgacg caatggcgct actgtggtca gccattgcgg
    77281 cgggtctgtc gatgggcgct tcgttactgg caaaagggat atttcatgcc gaactggaag
    77341 gtgtgccggg cagcttcttg ctggagaatc tcggttatac ctttggtttt attatcgtca
    77401 taatggcccg ccagcaatta tttaccgaaa acaccgtgac tgcggtacta cccgtcatgc
    77461 aaaaaccgac aatgagcaac gttggcttac ttatgcggtt atggggagtc gtgctgctgg
    77521 gtaatattct cgggactggt attgctgcgt gggcatttga atatatgcct atcttcaatg
    77581 aagaaactcg cgatgcattt gtcaaaatcg gcatggatgt gatgaagaac acccccagcg
    77641 agatgtttgc caacgcgatc atttccggct ggctgatcgc cactatggta tggatgtttc
    77701 ctgcagcagg tgcggcaaag attgtggtga ttatattgat gacctggctt attgccctgg
    77761 gtgacaccac ccatatcgtg gtcggttctg ttgaaatcct ctatctggtg tttaacggta
    77821 cgctgcactg gagcgatttc atctggccct tcgcactacc tactttagcg gggaacatct
    77881 gcggcggcac ctttatcttc gcgttaatga gtcatgcaca gattcgtaac gacatgagca
    77941 acaagcgtaa agcagaagca cgccaaaaag cagaacgtgc ggaaaacatt aagaaaaatg
    78001 ataaaaaccc agcataaatg gcgagggttt aagcaatcga gcggcagcgt acttaccccg
    78061 cagtccatta gcgggtatac tcgccgcatt gtcctcttag ttaaatggat ataacgagcc
    78121 cctcctaagg gctaattgca ggttcgattc ctgcagggga caccatttat cagttcgctc
    78181 ccatccgtac cagtccgcaa aatcccctga atatcaagcc ttccgtatat tcacagttcg
    78241 tcatggttcg cgtcagatcg ttgacagccg cactccatga cgggtaaaaa gtggataaaa
    78301 taattttacc caccggattt ttacccatgc tcaccgttaa gcagattgaa gcagcaaagc
    78361 cgaaagaaaa accataccgc cttctcgatg gtaatggcct gtacctttat gtccctgtat
    78421 ccgggaaaaa ggtatggcag cttcgctaca agattgacgg taaggagaaa atcctgaccg
    78481 tcggaaaata tccgcttatg actttgcagg aggcaaggga taaagcatgg actgcgagga
    78541 aagacatctc ggctggcatc gatccggtaa aggcgaaaaa ggcttcgtct aacaacaatt
    78601 catttagtgc gatttacaag gaatggtacg aacacaagaa gcaagtctgg tcagtagggt
    78661 atgcgactga acttgcaaaa atgtttgatg acgacatttt acccatcatc ggcggccttg
    78721 agattcagga tattgagccg atgcaactgc tggaagtaat ccgcagattt gaagatcgcg
    78781 gtgc
//
LOCUS       DAEEGA010000003        75863 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEEGA010000003 DAEEGA010000000
VERSION     DAEEGA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75863
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.003"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..211)
                     /locus_tag="KLH46_000165"
     CDS             complement(<1..211)
                     /locus_tag="KLH46_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377010.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRY"
     gene            complement(213..646)
                     /locus_tag="KLH46_000166"
                     /pseudo
     CDS             complement(213..646)
                     /locus_tag="KLH46_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(636..836)
                     /locus_tag="KLH46_000167"
     CDS             complement(636..836)
                     /locus_tag="KLH46_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBE0377011.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            966..1145
                     /locus_tag="KLH46_000168"
     CDS             966..1145
                     /locus_tag="KLH46_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377012.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            1270..1599
                     /locus_tag="KLH46_000169"
     CDS             1270..1599
                     /locus_tag="KLH46_000169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377013.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(1649..1837)
                     /locus_tag="KLH46_000170"
     CDS             complement(1649..1837)
                     /locus_tag="KLH46_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBE0377014.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(2212..3440)
                     /locus_tag="KLH46_000171"
                     /pseudo
     CDS             complement(2212..3440)
                     /locus_tag="KLH46_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            3689..4810
                     /gene="roxA"
                     /locus_tag="KLH46_000172"
                     /gene_synonym="ycfD"
     CDS             3689..4810
                     /gene="roxA"
                     /locus_tag="KLH46_000172"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBE0377015.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(4859..6085)
                     /gene="pepT"
                     /locus_tag="KLH46_000173"
     CDS             complement(4859..6085)
                     /gene="pepT"
                     /locus_tag="KLH46_000173"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBE0377016.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            6335..7471
                     /gene="potA"
                     /locus_tag="KLH46_000174"
     CDS             6335..7471
                     /gene="potA"
                     /locus_tag="KLH46_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBE0377017.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            7455..8312
                     /gene="potB"
                     /locus_tag="KLH46_000175"
     CDS             7455..8312
                     /gene="potB"
                     /locus_tag="KLH46_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBE0377018.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            8309..9103
                     /gene="potC"
                     /locus_tag="KLH46_000176"
     CDS             8309..9103
                     /gene="potC"
                     /locus_tag="KLH46_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBE0377019.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            9100..10146
                     /gene="potD"
                     /locus_tag="KLH46_000177"
     CDS             9100..10146
                     /gene="potD"
                     /locus_tag="KLH46_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBE0377020.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(10302..11123)
                     /gene="cobB"
                     /locus_tag="KLH46_000178"
     CDS             complement(10302..11123)
                     /gene="cobB"
                     /locus_tag="KLH46_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBE0377021.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(11139..12050)
                     /gene="nagK"
                     /locus_tag="KLH46_000179"
     CDS             complement(11139..12050)
                     /gene="nagK"
                     /locus_tag="KLH46_000179"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBE0377022.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(12079..13323)
                     /gene="lolE"
                     /locus_tag="KLH46_000180"
     CDS             complement(12079..13323)
                     /gene="lolE"
                     /locus_tag="KLH46_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBE0377023.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(13323..14024)
                     /gene="lolD"
                     /locus_tag="KLH46_000181"
     CDS             complement(13323..14024)
                     /gene="lolD"
                     /locus_tag="KLH46_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBE0377024.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(14017..15216)
                     /gene="lolC"
                     /locus_tag="KLH46_000182"
     CDS             complement(14017..15216)
                     /gene="lolC"
                     /locus_tag="KLH46_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBE0377025.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            15478..16551
                     /locus_tag="KLH46_000183"
     CDS             15478..16551
                     /locus_tag="KLH46_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBE0377026.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            16679..20125
                     /gene="mfd"
                     /locus_tag="KLH46_000184"
     CDS             16679..20125
                     /gene="mfd"
                     /locus_tag="KLH46_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBE0377027.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            20268..21230
                     /gene="ldtC"
                     /locus_tag="KLH46_000185"
     CDS             20268..21230
                     /gene="ldtC"
                     /locus_tag="KLH46_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HBE0377028.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(21313..21525)
                     /locus_tag="KLH46_000186"
     CDS             complement(21313..21525)
                     /locus_tag="KLH46_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE0377029.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            21810..22442
                     /gene="comR"
                     /locus_tag="KLH46_000187"
     CDS             21810..22442
                     /gene="comR"
                     /locus_tag="KLH46_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBE0377030.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTPLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(22504..23043)
                     /locus_tag="KLH46_000188"
     CDS             complement(22504..23043)
                     /locus_tag="KLH46_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBE0377031.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(23270..24574)
                     /gene="ndh"
                     /locus_tag="KLH46_000189"
     CDS             complement(23270..24574)
                     /gene="ndh"
                     /locus_tag="KLH46_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBE0377032.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISTAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(24982..25524)
                     /locus_tag="KLH46_000190"
     CDS             complement(24982..25524)
                     /locus_tag="KLH46_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE0377033.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(25547..26572)
                     /gene="nagZ"
                     /locus_tag="KLH46_000191"
     CDS             complement(25547..26572)
                     /gene="nagZ"
                     /locus_tag="KLH46_000191"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBE0377034.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(26583..27407)
                     /gene="thiK"
                     /locus_tag="KLH46_000192"
     CDS             complement(26583..27407)
                     /gene="thiK"
                     /locus_tag="KLH46_000192"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBE0377035.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(27388..28029)
                     /gene="lpoB"
                     /locus_tag="KLH46_000193"
     CDS             complement(27388..28029)
                     /gene="lpoB"
                     /locus_tag="KLH46_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBE0377036.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(28044..28421)
                     /locus_tag="KLH46_000194"
     CDS             complement(28044..28421)
                     /locus_tag="KLH46_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBE0377037.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(28424..28783)
                     /gene="hinT"
                     /locus_tag="KLH46_000195"
     CDS             complement(28424..28783)
                     /gene="hinT"
                     /locus_tag="KLH46_000195"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBE0377038.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            29122..31307
                     /gene="fhuE"
                     /locus_tag="KLH46_000196"
                     /pseudo
     CDS             29122..31307
                     /gene="fhuE"
                     /locus_tag="KLH46_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(31367..32800)
                     /gene="ptsG"
                     /locus_tag="KLH46_000197"
     CDS             complement(31367..32800)
                     /gene="ptsG"
                     /locus_tag="KLH46_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBE0377039.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(33095..33892)
                     /locus_tag="KLH46_000198"
     CDS             complement(33095..33892)
                     /locus_tag="KLH46_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBE0377040.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(33903..34907)
                     /gene="holB"
                     /locus_tag="KLH46_000199"
     CDS             complement(33903..34907)
                     /gene="holB"
                     /locus_tag="KLH46_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBE0377041.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(34904..35545)
                     /locus_tag="KLH46_000200"
     CDS             complement(34904..35545)
                     /locus_tag="KLH46_000200"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBE0377042.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(35535..36557)
                     /gene="yceG"
                     /locus_tag="KLH46_000201"
     CDS             complement(35535..36557)
                     /gene="yceG"
                     /locus_tag="KLH46_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBE0377043.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(36560..37369)
                     /gene="pabC"
                     /locus_tag="KLH46_000202"
     CDS             complement(36560..37369)
                     /gene="pabC"
                     /locus_tag="KLH46_000202"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBE0377044.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(37489..38730)
                     /gene="fabF"
                     /locus_tag="KLH46_000203"
     CDS             complement(37489..38730)
                     /gene="fabF"
                     /locus_tag="KLH46_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBE0377045.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(38817..39053)
                     /gene="acpP"
                     /locus_tag="KLH46_000204"
     CDS             complement(38817..39053)
                     /gene="acpP"
                     /locus_tag="KLH46_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBE0377046.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(39264..39998)
                     /gene="fabG"
                     /locus_tag="KLH46_000205"
     CDS             complement(39264..39998)
                     /gene="fabG"
                     /locus_tag="KLH46_000205"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBE0377047.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(40011..40940)
                     /gene="fabD"
                     /locus_tag="KLH46_000206"
     CDS             complement(40011..40940)
                     /gene="fabD"
                     /locus_tag="KLH46_000206"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBE0377048.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(40956..41909)
                     /gene="fabH"
                     /locus_tag="KLH46_000207"
     CDS             complement(40956..41909)
                     /gene="fabH"
                     /locus_tag="KLH46_000207"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBE0377049.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(41977..43047)
                     /gene="plsX"
                     /locus_tag="KLH46_000208"
     CDS             complement(41977..43047)
                     /gene="plsX"
                     /locus_tag="KLH46_000208"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBE0377050.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(43128..43301)
                     /gene="rpmF"
                     /locus_tag="KLH46_000209"
     CDS             complement(43128..43301)
                     /gene="rpmF"
                     /locus_tag="KLH46_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBE0377051.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(43353..43874)
                     /gene="yceD"
                     /locus_tag="KLH46_000210"
     CDS             complement(43353..43874)
                     /gene="yceD"
                     /locus_tag="KLH46_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBE0377052.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            44073..44657
                     /gene="yceF"
                     /locus_tag="KLH46_000211"
     CDS             44073..44657
                     /gene="yceF"
                     /locus_tag="KLH46_000211"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBE0377053.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(44769..45728)
                     /gene="rluC"
                     /locus_tag="KLH46_000212"
     CDS             complement(44769..45728)
                     /gene="rluC"
                     /locus_tag="KLH46_000212"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBE0377054.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            46301..49486
                     /gene="rne"
                     /locus_tag="KLH46_000213"
     CDS             46301..49486
                     /gene="rne"
                     /locus_tag="KLH46_000213"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBE0377055.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACTSPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(49682..50635)
                     /gene="flgL"
                     /locus_tag="KLH46_000214"
     CDS             complement(49682..50635)
                     /gene="flgL"
                     /locus_tag="KLH46_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBE0377056.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(50647..52289)
                     /gene="flgK"
                     /locus_tag="KLH46_000215"
                     /pseudo
     CDS             complement(50647..52289)
                     /gene="flgK"
                     /locus_tag="KLH46_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(52355..53296)
                     /gene="flgJ"
                     /locus_tag="KLH46_000216"
     CDS             complement(52355..53296)
                     /gene="flgJ"
                     /locus_tag="KLH46_000216"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBE0377057.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(53296..54393)
                     /gene="flgI"
                     /locus_tag="KLH46_000217"
     CDS             complement(53296..54393)
                     /gene="flgI"
                     /locus_tag="KLH46_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBE0377058.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(54405..55103)
                     /gene="flgH"
                     /locus_tag="KLH46_000218"
     CDS             complement(54405..55103)
                     /gene="flgH"
                     /locus_tag="KLH46_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBE0377059.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(55156..55938)
                     /gene="flgG"
                     /locus_tag="KLH46_000219"
     CDS             complement(55156..55938)
                     /gene="flgG"
                     /locus_tag="KLH46_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBE0377060.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(56076..56827)
                     /gene="flgF"
                     /locus_tag="KLH46_000220"
                     /pseudo
     CDS             complement(56076..56827)
                     /gene="flgF"
                     /locus_tag="KLH46_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(56847..58055)
                     /gene="flgE"
                     /locus_tag="KLH46_000221"
     CDS             complement(56847..58055)
                     /gene="flgE"
                     /locus_tag="KLH46_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBE0377061.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(58080..58775)
                     /gene="flgD"
                     /locus_tag="KLH46_000222"
     CDS             complement(58080..58775)
                     /gene="flgD"
                     /locus_tag="KLH46_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBE0377062.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(58787..59190)
                     /gene="flgC"
                     /locus_tag="KLH46_000223"
                     /pseudo
     CDS             complement(58787..59190)
                     /gene="flgC"
                     /locus_tag="KLH46_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(59194..59610)
                     /gene="flgB"
                     /locus_tag="KLH46_000224"
     CDS             complement(59194..59610)
                     /gene="flgB"
                     /locus_tag="KLH46_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBE0377063.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            59765..60424
                     /gene="flgA"
                     /locus_tag="KLH46_000225"
     CDS             59765..60424
                     /gene="flgA"
                     /locus_tag="KLH46_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBE0377064.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            60500..60793
                     /gene="flgM"
                     /locus_tag="KLH46_000226"
     CDS             60500..60793
                     /gene="flgM"
                     /locus_tag="KLH46_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBE0377065.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            60798..61214
                     /gene="flgN"
                     /locus_tag="KLH46_000227"
     CDS             60798..61214
                     /gene="flgN"
                     /locus_tag="KLH46_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBE0377066.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(61254..62789)
                     /gene="murJ"
                     /locus_tag="KLH46_000228"
     CDS             complement(61254..62789)
                     /gene="murJ"
                     /locus_tag="KLH46_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBE0377067.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(62956..63822)
                     /locus_tag="KLH46_000229"
     CDS             complement(62956..63822)
                     /locus_tag="KLH46_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE0377068.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(63824..64471)
                     /locus_tag="KLH46_000230"
                     /pseudo
     CDS             complement(63824..64471)
                     /locus_tag="KLH46_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
     gene            complement(64482..65066)
                     /gene="rimJ"
                     /locus_tag="KLH46_000231"
     CDS             complement(64482..65066)
                     /gene="rimJ"
                     /locus_tag="KLH46_000231"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBE0377069.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            65302..66504
                     /gene="mdtH"
                     /locus_tag="KLH46_000232"
     CDS             65302..66504
                     /gene="mdtH"
                     /locus_tag="KLH46_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBE0377070.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHRLM
                     AGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSYMG
                     FSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKRAA
                     RRLLERDA"
     gene            66568..67215
                     /gene="grxB"
                     /locus_tag="KLH46_000233"
     CDS             66568..67215
                     /gene="grxB"
                     /locus_tag="KLH46_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBE0377071.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            67349..67909
                     /locus_tag="KLH46_000234"
     CDS             67349..67909
                     /locus_tag="KLH46_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE0377072.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            68015..69061
                     /gene="pyrC"
                     /locus_tag="KLH46_000235"
     CDS             68015..69061
                     /gene="pyrC"
                     /locus_tag="KLH46_000235"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBE0377073.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            69135..69380
                     /gene="dinI"
                     /locus_tag="KLH46_000236"
     CDS             69135..69380
                     /gene="dinI"
                     /locus_tag="KLH46_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBE0377074.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            69670..69924
                     /gene="bssS"
                     /locus_tag="KLH46_000237"
     CDS             69670..69924
                     /gene="bssS"
                     /locus_tag="KLH46_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBE0377075.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            70039..71157
                     /gene="solA"
                     /locus_tag="KLH46_000238"
     CDS             70039..71157
                     /gene="solA"
                     /locus_tag="KLH46_000238"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBE0377076.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            71205..71318
                     /locus_tag="KLH46_000239"
     CDS             71205..71318
                     /locus_tag="KLH46_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HBE0377077.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            71579..72145
                     /locus_tag="KLH46_000240"
     CDS             71579..72145
                     /locus_tag="KLH46_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBE0377078.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            72149..72724
                     /locus_tag="KLH46_000241"
     CDS             72149..72724
                     /locus_tag="KLH46_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBE0377079.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(72766..73818)
                     /locus_tag="KLH46_000242"
     CDS             complement(72766..73818)
                     /locus_tag="KLH46_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBE0377080.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            74043..74963
                     /gene="lpxL"
                     /locus_tag="KLH46_000243"
     CDS             74043..74963
                     /gene="lpxL"
                     /locus_tag="KLH46_000243"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBE0377081.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            75135..75332
                     /locus_tag="KLH46_000244"
     CDS             75135..75332
                     /locus_tag="KLH46_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBE0377082.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            75356..>75863
                     /locus_tag="KLH46_000245"
     CDS             75356..>75863
                     /locus_tag="KLH46_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE0377083.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKV"
BASE COUNT        18494 a        20066 c        18762 g        18541 t
ORIGIN      
        1 tatatctgtc gtgcaataga tctatttctt gcagttccat tattacaggc tcaagaagcg
       61 ttattaatgc cgtggcaatt cttgattttt gtttgtcgcg ttcattgtcg ccaagtgttt
      121 caagccatat gcgcaatatt tccagcatgt tttcactgtg agaaagtgca agaaatgcgc
      181 ggtctattgt ttcgtggtaa atatcacgca tattaatccc cgtccgtcgt ttttcttaaa
      241 ataacctctg tcacgaaatc agcataatcg gcggcgatat tcagtacatc aagcccgttg
      301 atttatattc tctcgtggaa agtaagaaaa aagccgctct aataagttct ggcattgacg
      361 aaagcgcatc agccgcatca tcaggaacgc cggaaaattc ctgtttcagg gaattaaaac
      421 gatcatcacg cataaccccc ccattttcac aatcggcaac aagaatattt ttagcgtcat
      481 tcagtgaccg cgttgcgatg tatcggatac attccagggc gaactgtgtc cggtctttct
      541 gttcttctcc ctgcgcaagc tctgccgtgc attcaatatc aataagcgcg tgcatcagcg
      601 tagtgagtgc ggcggctgct gcgttcggtg tggttttatt gtgcataggt ttccccctga
      661 cgaatacggg cggcgaatac agcaacacaa ccggacgggc aacggctacg cgcttcgcgt
      721 tccgtccaag cggttacgtg gatgatttgg gattctccgg cactcagtgc cagaaaacgc
      781 cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg
      841 gctcctagtt tacgttgttg gttagaagcc ccgttactgc tcctacagtg cggggctttg
      901 ctttttgtgt tgattgtgtg taatgtgtca ctacacataa atacattacc tgaggtgtaa
      961 ttaacgtgtc aacacacaaa aacgaaaggc gtggtaatcc tccgttccag ttccgtttag
     1021 atcctgactt acgggaaaag atggaaaagg cacaacaaca ggacggcgat gattctcttg
     1081 ctgcctggat aaagcgaata atccgcaaag aattacagca acgcggcata gaacccaaag
     1141 gataaaaact atcagcgccg tggtgtgagg tactacggcg cattgcttta caggcgcaca
     1201 caatgaccaa cgaagaatca accgaaaaaa catcacctgc acgaaaaaga cgacgcagaa
     1261 agaaagagca tgaatcagaa cgattcgcgc cgtgttcttt tgcccttgag aagtacctta
     1321 aagaatactc atcaaaggag agaacttcgc agttatggca acgcactgaa ccagactgat
     1381 tgcattgccc accagcctga tagcggctat catccccgtg cttatgtttg ggatcacaca
     1441 cacaaacggc gcagcgggtt atctgttcag aaaggcggct ccatttcggg gccgcttttt
     1501 ttatgcctga aaaaccccaa ttttgtggtt ttccagtttc accagggcga acgaatcccc
     1561 gcccacgttc tggcgtatat tcaatcttca tggttatagc tctgtgttca gatgattgat
     1621 gtgtggcggc tgcgtgccgc cagcgtgatt aatgaactgc cttgcagcta tccttccagg
     1681 ccagaacctc ggataaagac cagccaacgg aacgaccgcc aagtttacga cgtgatggga
     1741 attgtccggc cttttccagg cggtagcggc atgagcggct aaggcctgtt agcttttcgc
     1801 attctttttc acgtataaac cgatcagtgc ttaacactat tgcccccttt cgtttcttaa
     1861 agagttattt cgtgttctat tgcgttggga tgtgtctgat tgtgtcagga tgattcagag
     1921 ttggcaaatg ttgaggccgc atggtttaca gaaagaggaa taatcaggat aaaatccttt
     1981 aaattcatgt taatacaaag gcataaaata ttgtttcatg ccttttttct cgctatttaa
     2041 agagtgattc gctagtgtat aaaaaatcag caacacatta aaaatcagct atttataaat
     2101 ctgtacactt tttcacctct tgttcgtagt tgttccgctt tgttgctcat tgttgcacgt
     2161 tgtatctgtt cgcatatcca gtatgcgcat actgaaaaaa cacgaaaaaa attattttct
     2221 tctggctact ggtagcgtgg ttacgttttc atgtgttccc gccagtatcc ctaaccgctc
     2281 cgtccacata tccagcgcat tgcgtttagc atccagataa cgggaatgat tataaactcg
     2341 ctgcattcct ggcatctggt ggcctgtaag ctgctccacg acgtgaggat ccacgcctaa
     2401 atcgttcagc atcgttgtaa aggtgcgccg gatgtcatgt agtgaccagt gagggtgatt
     2461 tagcctcctg tgcgctaatc tgccgtactg cgacacgctt gtttcctgtt tcacttcccc
     2521 cagcaataag cccgtgtgcc tgttctgctc caccagctgc gtgacgaacg gcagtattgc
     2581 ttccggtatg ggccggaata ttgccacctt cgttttgctg tgttctttcg gcacggtcca
     2641 gagcatttcg gtaaaatccc actcgctgat ctccgataac ctcagttcta ccgtccggca
     2701 tccgaacaca atcaggaggc ggattaacgc gatgtagtag ggggagaata tttttttgtc
     2761 cagtgcctgc aataattcgc ccagttcttt ggtgcttaag acacgctcgc ttatatccgg
     2821 ttttttccca acgtccgcca cactcatatc atcaagaacg ttgctgattg catagcgccg
     2881 cctccggcag aacttaagcg cctgtttgca cgtctgtagc aagaatccgg cagtaacagg
     2941 cgttcgcttt gccacctggt caaaacaggc cagccagtgc cgtagctcgc atttatccag
     3001 cggcatagca ccaatgtgct gtattacgtg attattaagg cgctttttca gggcggcata
     3061 atccacgcgg ttttcctttg cgtacgactc aagccagtag gtgagcgcat cgccaaccgt
     3121 taccggcttt aacgcttcct gtacggtgta attaagctca tgacgtggat tttcccctct
     3181 gccagccatg cgcgacactg ggcggctttt tccctggctg atttcaggtt cagatcagga
     3241 taatttccca gcttaatgcg ttccggtggt gccccccttc ccgttccggc cctgtaagtg
     3301 aaataccagg ttaaaaggcc actggttgaa tgcctgacgc tcaggtttcc accgtcatta
     3361 agaaaggctg ttttttgggc gggtgtgccg ttgattttcc tcagctgtgt atcgctcagt
     3421 ttgttaagtg ctctgctcat aattttgatt ccggtcatca caattaacta caccattaac
     3481 tacaccgatc gttgcacaaa gggctacaac gtgaaacaag ctggaacaag gaaaatcaca
     3541 aatctgttta taatcaaaaa cataatgaac aatctgaaac attatgaaac ggcaaaaaac
     3601 actaattgat aaaatatgtc catacttcac gcattacgtt aagcatccgt tataatcggt
     3661 tgcagatacc agcctgtgga tgcttaacat ggaataccaa ctcactctta actggcccga
     3721 ttttcttgaa cgtcactggc agaaacgccc ggtggtgtta aaacgcggct ttaataattt
     3781 tattgacccg atctctccag acgagttggc gggtctggcg atggaaagcg aagttgacag
     3841 tcgactggtc agtcaccagg atggcaaatg gcaggtcagc cacggcccgt tcgaaagcta
     3901 cgatcatctc ggtgaaacca actggtcatt actggtacag gcagtaaacc actggcatga
     3961 gccgaccgcc gcgctgatgc gaccgttccg tgaactaccg gactggcgta ttgatgatct
     4021 gatgatttct ttttctgtac ctggcggcgg cgtcggcccg catctcgatc agtacgacgt
     4081 gtttatcatt cagggtaccg gacgtcgtcg ctggcgagtg ggcgaaaagc tgcaaatgaa
     4141 acagcactgc ccacatccgg atctgttaca ggtcgatccg ttcgaagcca tcatcgatga
     4201 agagctggag cctggtgata ttctttatat tccgccagga ttcccgcatg aaggctacgc
     4261 gctggaaaat gcgatgaact attccgtggg ttttcgcgcg ccaaatacgc gggaattaat
     4321 tagcggattt gccgattatg tgctgcaacg tgaactgggc ggcaactact acagcgatcc
     4381 ggatgttcca cctcgtgctc atcctgcgga tgttctgccg caagagatgg ataaactgcg
     4441 tgagatgatg ctcgaattga tcaaccagcc ggaacacttt aagcaatggt ttggcgagtt
     4501 tatatcccag tcacgtcatg aactggatat cgcgccgccg gaaccgcctt atcagccaga
     4561 tgaaatctac gatgcgctga aacaaggtga tgtgctggtg cgcctgggtg gtctgcgcgt
     4621 attgcgcatt ggcgacgacg tgtatgccaa tggtgagaag atcgattccc cgcaccgtcc
     4681 ggcactggat gcactcgcca gcaacattgc gctgactgcg gagaattttg gcgatgcgct
     4741 ggaagatccg tcattcctcg cgatgctcgc ggcgctggtc aatagcgggt attggttctt
     4801 cgaagggtaa gtttggattt aagccggatg cagcatcaca tgccgcatcc cttttcgctt
     4861 acttccgttg cgccgttaac tcggcaatac ggacgatcac ctgcaccgct ttttccatac
     4921 cttccagaat cacaaactca tgcttaccat gatagttgta accgccagtg aacaggttcg
     4981 ggcaaggtaa tcccataaac gacaactgcg cgccgtcggt accgccgcgg atcggtttca
     5041 gttccggttc aatatcgcag tcacgcatcg cctgctgggc gatatcgaga atatgcggat
     5101 gctcaaccac tttctcgcgc atattgtagt aactgtcttc aatcaccaat tcaatgtagc
     5161 aatcaggatg taaccctttg cccacttttt tggcgatctc catcatttta cgtttacgcg
     5221 cttcaaactg tttacggtcg aaatcacgga tgatgtagtg catatcggcc cgttcaacgg
     5281 tgcctttcat gctcgccagg tgatagaaac cttcatagcc ttctgtcatt tccgggcttt
     5341 catccgccgg aacttccgca tgaatacgtg ccgccagcga cagcgcattt accatcactc
     5401 ctttcgccgt gcccggatga acattgttac cgacaatttt tatattgacc gatgcggcgt
     5461 tgaagttttc aaactccagt tcgcctacgc caccaccgtc aacagtgtaa gcccagcggg
     5521 catcgaaggc atcaacatca aaatgtttcg cccctttgcc cacttcttca tccggggtaa
     5581 aggcgacgcg aatatcacca tgcggaatgt ttttctgttg caataccgcc agcgcggtca
     5641 tgatttctgc aatacctgct ttgtcatcag cacctaacaa ggttttacca tcggtggtaa
     5701 tcaacgtctg acccagtagc tgatgcagca ccgggaacat aaccggtgat aaaacttcat
     5761 cgccgatacc cagcgcaata tcgccaccgc gatagttttc aacaatttgc ggattaacat
     5821 ttttgccgct gcaatccggt gaggtatcca catgagaaat aaagccaatc gccgggatat
     5881 cgccagggac gttagccggt aacgtcgcca tcaaagtgcc cttctcactt aaggtcacat
     5941 tgataagccc catttcttcg agctgctctt tcagcagatg caataacttc cattggcctt
     6001 ccgtgctggg aacctgtctc acccctgctt ttgattgggt atccagagac acgtagttca
     6061 aaaatcgctc aagtagttta tccatgtagt caccctcact ttttgtgaca acattattaa
     6121 gaagcaagaa aagacaaata ttgcgtcagg tcacttttac ccctgcaagc gggaatattt
     6181 atcagcatta ccttaatgaa tataaagcta agccagtaat tcacaacaaa gattggcgat
     6241 tcaagcggtt tgccgtagaa ttaccgccct cattaagagt caccaaggtg gttaaccaca
     6301 aaccccgcat cggtaagcca tccgttgcgt ttacatggga cagagtaaaa aattgaataa
     6361 acaaccgagt tcgctttcac cgctggtgca attggcggga attcgcaaat gctttgatgg
     6421 taaagaggtc attccccagc tggatctgac tatcaacaat ggcgagttcc tcacgctgct
     6481 tggcccttct ggctgcggta aaacaaccgt tcttcgcctg attgcaggtc tggaaactgt
     6541 tgattccgga cgcatcatgc tggataacga ggacatcacc cacgttccgg cggaaaaccg
     6601 ctatgtgaac actgttttcc aaagctatgc acttttcccc cacatgaccg tgttcgaaaa
     6661 tgtggccttt gggttgcgca tgcaaaaaac ccccgctgct gaaattacgc cccgcgtgat
     6721 ggaagccctg cggatggtgc agctggaaac cttcgctcaa cgtaaaccgc atcagctctc
     6781 tggtggtcaa cagcaacgcg tcgccattgc tcgcgcggtg gttaacaagc ctcgtctgtt
     6841 gttgctggat gagtcgctct cagcgctgga ttacaaactg cgtaagcaaa tgcagaacga
     6901 gctgaaagcg ttacagcgta agcttggcat tacattcgtt ttcgtgactc acgaccagga
     6961 agaagcactc accatgtcgg acaggattgt ggtgatgcgc gatggtcgca ttgagcaaga
     7021 cggcacgccg cgtgaaatct acgaagagcc gaaaaacctg tttgttgccg ggttcattgg
     7081 cgaaattaat atgtttaacg ccacggtgat cgagcgactc gacgagcagc gcgtacgcgc
     7141 caacgttgaa ggccgcgaat gtaatatcta cgttaacttc gccgttgaac cggggcaaaa
     7201 actgcatgtt ctgctgcgcc cggaagactt acgtgttgaa gagattaacg acgacaacca
     7261 cgctgaaggg ctgattggtt acgttcgtga gcgtaactac aaaggcatga cgctggagtc
     7321 ggttgttgaa ctggaaaatg gcaagatggt gatggtcagc gaattcttca atgaagacga
     7381 tcctgacttt gaccactctc tcgaccaaaa aatggccatt aattgggtag aaagctggga
     7441 ggtcgtactg gctgatgaag aacacaagta agttccagaa tgtagtgatt gtcactattg
     7501 tcggttggct tgtgttgttt gtctttctgc ccaacctgat gatcattggc accagctttt
     7561 tgacccgcga cgacgccagt ttcgtcaaaa tggtctttac gctggataac tacacgcgtc
     7621 tgctcgatcc gctctatttt gaagtgctat tgcactcgct gaatatggcg ctgatcgcca
     7681 ccctcgcctg cctggtgctg ggctatccat ttgcctggtt tctggcgaag ctgccacaca
     7741 aggtgcgtcc gctgctgctg tttctgctga ttgttccgtt ctggaccaac tcattgattc
     7801 gtatctacgg gctgaaaatt ttcctcagca ccaaaggcta tctcaacgag tttttgctct
     7861 ggctgggcgt tatcgacaca ccaatccgta tcatgttcac gcccagtgcg gtgattatcg
     7921 gtctggttta cattctgctg ccgtttatgg tgatgccgct gtactccagt atcgaaaaac
     7981 tggataagcc gttgcttgag gcggcgcgcg atctcggtgc cagcaagtta cagactttta
     8041 tccgcatcat tattccactg acgatgccgg ggattattgc cggatgtctg ctggtgatgc
     8101 tgccagcgat gggcctgttc tatgtgtccg acctgatggg cggtgcgaaa aacctgctga
     8161 tcggtaacgt catcaaggta cagttcctca atattcgtga ctggccgttt ggtgcagcca
     8221 ccagcattac gctgactatc gtaatgggcc tgatgttgct ggtttactgg cgcgcttctc
     8281 gtttgctgaa taagaaggtg gaactcgaat gatcggtcga ctgcttcgcg gcggttttat
     8341 gaccgctatc tacgcgtacc tgtatatccc aatcattatt ttgattgtga actcctttaa
     8401 cagctcgcgc tttggcatca actggcaggg ttttaccacc aaatggtata gcctgctgat
     8461 gaacaacgac agcctgttac aggcagcgca gcattcacta acaatggcgg tgttttcggc
     8521 gacgtttgct acgcttatcg gttcactgac ggcagttgcg ctgtaccgtt atcgctttcg
     8581 cggtaagccg ttcgttagcg gaatgctgtt tgtggtaatg atgtcaccag atatcgtgat
     8641 ggcaatttcg ctgctggtgc tgtttatgct gctgggtatt cagcttggct tctggtcgct
     8701 gctgttctcg catatcacct tctgcctgcc atttgtggtg gtgacggtgt attcgcgcct
     8761 gaaaggtttt gacgtgcgga tgctggaagc ggcgaaagat ctcggtgcca gcgaatttac
     8821 cattctgcga aaaatcattc tgccactggc aatgccagcg gtggcggcgg gctgggtgtt
     8881 aagctttacc ctgtcgatgg acgatgtggt agtttcttca ttcgtcaccg gaccaagtta
     8941 tgaaattctg ccgttaaaaa tttattcgat ggtcaaagtc ggcgtatcgc cggaagttaa
     9001 cgcgctggca accatattac tggtgctgtc gctggtgatg gttattgcca gccagcttat
     9061 tgctcgtgat aaaacgaaag gtaacacagg ggacgttaaa tgaaaaaatg gtcacgccac
     9121 ctgctcgcgg cgggtgctct ggcactgggc atgagcgccg ctcacgccga tgacaacaac
     9181 acgctgtatt tctacaactg gaccgagtac gtgccgccag gactgcttga acagttcacc
     9241 aaagaaaccg gtattaaggt tatctattcg acttacgagt cgaacgaaac catgtacgcc
     9301 aagctgaaaa cgtacaaaga cggtgcctat gacctggtgg ttccttcaac ctattacgtc
     9361 gataaaatgc gtaaagaggg gatgatccag aagatcgaca agtcgaagtt aacgaacttc
     9421 agcaatctcg atccagacat gctcaacaag ccttttgacc cgaataacga ctactccatt
     9481 ccgtatatct ggggtgcgac ggcgattggt gttaacggtg atgcggtgga tccgaaatct
     9541 gtcaccagct gggccgatct gtggaagcca gagtacaaag gcagcctgct gttgaccgac
     9601 gatgcccgtg aagtgttcca gatggcgctg cgtaagctgg gctactccgg taacaccacc
     9661 gatccgaaag agattgaagc tgcatataac gagctgaaaa aactgatgcc aaacgtcgca
     9721 gcgtttaact ccgataaccc ggctaacccg tacatggaag gcgaagttaa cctcggcatg
     9781 atctggaacg gttctgcttt cgttgcacgc caggcgggta cgcctattga cgtggtgtgg
     9841 ccgaaagaag gcggcatatt ctggatggac agcctggcga tcccggcaaa tgccaaaaac
     9901 aaagaaggcg cgctgaaatt gatcaacttc ctgctgcgcc cggatgtggc aaaacaggtt
     9961 gctgaaacta tcggttatcc aacgccaaac cttgcggcgc gtaagctgtt aagtccagaa
    10021 gtggcaaacg ataagacgct ctacccggat gctgaaacca ttaaaaatgg cgaatggcag
    10081 aatgacgttg gcgcagccag cagcatttat gaagagtatt atcagaagct gaaagcagga
    10141 cgttaattca gcaagcgggc ggtggtatta ccgcccgttt tttagttagc aaggatgtgg
    10201 ctaatgagtc aggacagtaa agttttatcc cgctctttcg tcggtattgt ggtccgcttg
    10261 taggcctgat aagcgtagcg catcaggcaa tgcttcctgc tttataatcc ctccagcaac
    10321 ttttcaacaa attctggcac cacctggctt gccgggccgt aatatttctc ggcaaattca
    10381 ttaccaacct gactcggttc aagattcagt tcaacggtgt gagcaccatg tagtttggct
    10441 tcgtgtacaa acccagccgc cggatagaca tgcccggaag taccaatggc aataaagaca
    10501 tccgccatcg acaacgccat ataaatttca tccatgccga gtggcatttc gccaaaccaa
    10561 acgacgtgcg ggcgcaaggg tgccgggaac tggcaacaat ggcatttatc ttctggcgta
    10621 acgtctccgg tccagtcgag aacctgacca ctttgcgaac aacgaacttt cagcaattcc
    10681 ccatgcatat gaatgacatt ggtattacct gcgcgttcat gcaggttgtc tatattctgc
    10741 gttaccagca aaaagcgatc gccgagagcg tcttgcagtt tagccagcgc aagatgcgcg
    10801 gcgttaggct gaatttctgg ctgctgcagc tgtcgacgac gggcgttata aaacgcttgc
    10861 accagttcag gatcgcgatc gaaaccttcc ggcgttgcca catcttcaac ccgatgttct
    10921 tcccacaggc catctgcggc gcgaaaggta cgaatacctg attccgcaga aattcctgcc
    10981 cctgtcagta cgagtactct tggtttttcc attgcttccg gcaccacttt atctctgaaa
    11041 aaaatacgct gacgcaaacg ctcgcgcagg cgacgtttat ttttacgaaa acgacttaac
    11101 cgatgacccc gacgcgacag catagcaacc tctgttgttt aatcggttag atgtaggaag
    11161 gccgcaccac gcattcctcc cgcatcaccg tggcgcgcgc gttcaatgcg cggaacacga
    11221 gctacaggta agagatgaca aggcaacctg tccgccagtt gcgttgtgat tgccgggaaa
    11281 ttcgataagc caccaccaat gacgaccagg tcagggtcaa caatggtcag gatatttccc
    11341 agagaaaccg ctaataaatc cagataacgc tcaacgtgcg cccttgcctg ctcatcgcct
    11401 tgatcataaa gcgcaataat ttcgggagcc tgcaacggtt gatgataata gtgttgatac
    11461 agccacgcaa aaccgcgacc agacagataa ttttcaatgc agccatgctg accacagccg
    11521 cagcggcgta acgggaaatc caaccccatc atggttaacg catcaaccgg cagacgcata
    11581 tggccaaact cgccggtaat gtagctttta ccagtaatcg gtttgccgtt gaaaaccagc
    11641 ccgccgccaa cgccggtgcc gagaattaac cccatcacca gtggatattg cgtaaattca
    11701 tcatcccagg cttctgaaag ggcaaaacag ttggcatcgt tatcaaggcg tacatcgcga
    11761 tcaagacgtg cgctcaggtc ggcacgcagc ggtttgccgc tggcagcggg gacattagcg
    11821 gcatacagct taccatcttc tgtttccggc attcccggaa taccgatgcc gacagaacct
    11881 ttacagccaa aacgttggtc agcttcagct accagctcac acactgcatc taaaaatgcg
    11941 tcatagctgt cacgcggtgt cggcacccgc ttttcccact gcaactgccg accgctatca
    12001 aacacgccaa gcgcaatttt tgttccacca atatcaaacc cgtaatacat tgccgctcct
    12061 tgttttaatg tactgccttt actggccgct aaggactcgc gcagggtcaa tattactggc
    12121 gcgccgcgcc ggataccaac ttgccaaaag actcagcaac aatgctgtga ccagtacgta
    12181 gaagacgtcc agccagtgca attccgatgg caggaagtca ataaaataaa tatcgctgga
    12241 gaggaactga tgaccgatca gcttttcaat ccactcaata atcggggtaa gttgcagtga
    12301 aacaactacg ccgataacca ccccacacag gctgccgaac agccctgcca gcaatccata
    12361 ccagacaaag atggcgcgaa ttaaaccatc tttcgccccc agcgttctta atactgcgat
    12421 atcgccactc ttgtctttca ccgccatcac taaggtggag acgatgttga aacaggccac
    12481 gccaatcacc agtaccatcg ccagatacat aatggcgcgg atcatctgga tatcgcgata
    12541 catatagccg taagtaccaa tccaactttt aatataaaca tagctgttgg tcacttcacc
    12601 cgcatcgcgt accagcttat tggcgttgaa aacatccgtc attttaaggg caatacctga
    12661 cacgctggaa cccatatcaa gatattgttg ggcatccgcc agcgggatca tggcaaaact
    12721 gtgatcgagt tgaccactca actgcaaaat accggcaatg tgcaaacgca cacgttttgg
    12781 ctgcatcagt ttatgctcgg gattcgagtt ggggatcatg atcgacaccc aatcgccctg
    12841 cttcactttc agcgcatccg ccacgccttt gccgatgata atttgctgtt cgcccgcttt
    12901 aaaattgcgc cacgcatccc cctgaacaaa cgagggtaat gcgctcagac gctgttcctg
    12961 ttgtgggtta acgcccttca cctggattgc gcgcagattc gctccacttt ccaccagacc
    13021 ggtgaaattg atatacggcg cagcggcggc aatacccggc actttctgca cgttatccag
    13081 tgcttcctgc cagttagtcc atggctgatt taccgcttcg atttcgccat gcggcaccac
    13141 cgccagaatg cggttattca gttcgcgttc aaagccgttc atcgcgctta agccgacgat
    13201 caataccgcc acgccgaggg caatgccaat ggtagaaatg acagagatta gcgacaccat
    13261 gccgccgcgt cgccgtccgc gactaaaacg caggccaatc aataacgata aaggcatcgc
    13321 cattactccg cccccatcag gctcagttcc gccgtcagac gtccatcacg catttccagt
    13381 tggcggctca tacgtttcgc cagttgcagg tcgtgagtga ccaccaggaa agcggtgccc
    13441 tgcaagcgat tcaactcccc aagcaactgg aagatgctgt cggcgttacg cgcatcgagg
    13501 ttaccggtag gttcatccgc cagtaccaga cgcggattat tgaccagcgc acgggcaatc
    13561 gctacacgct ggcgttcgcc gccagaaagt tcagatgggc ggtgattcgc acgatgctcc
    13621 agccccaccg cttttaacat ctccagtgca cagctgttga tttcagcggg ctttttcttg
    13681 ccaatcagca gtggcatagc cacgttttcc agggcagtaa aatccggcag caggtggtga
    13741 aactgataaa taaagcccag cttctggttg cgcagttctg ctttcgccgc tgaagacagt
    13801 ttgctcatcg gctgaccgtt aaagatcaca tcgccggagg ttggcgtatc cagcccgcct
    13861 aacaggtgca gcaaggtact tttaccggaa ccagagctac cgacaattgc catcatttca
    13921 ccttcgccca cgctgaaact gacattgagc agcacatcgg tttgcacact gccttcctga
    13981 tagcgtttgc acaggttgtc gcattgcaac aggatcttat tcataacgta aagcctcagc
    14041 gggttgtgtg gcggcagcgc gccatgaagg gtaaagcgta gacagcagcg cgatagccat
    14101 cgccaccagc gcaataacaa tgacctgtaa aggttcgata gccaccggca gcgccgcgcc
    14161 atcaagcagg acgccgatta tcggcatcag attatttaac tggctggcaa gcagtgcgcc
    14221 aagcgccgct ccgaggattg caccgataat cccggcgcta gccccttgca ccataaagac
    14281 catcatgatt tgtcgcggag ttaagccttg cgtttgcagg atcgcgactt cgccctgctt
    14341 ctccatcacc atcagcccca gcgaggtaat aatgttaaac gccgcaacag cgacaatcag
    14401 gctcagcagt aagcccatca tatttttttc catgcgtacg gcctggaaca gctcgccttt
    14461 acggtcacgc cagtcctgcc atttgctgcc ttcaggcagt ttttgctgac ttaaagagtc
    14521 aactctcagc ggctcatcca gccacaaacg ccagccggta atattggctg ccggataacg
    14581 catcagacgc gatgcatcct caatattcac cagcatttca tagccatcga cttcactgtt
    14641 ggcggcgaaa gtgccaatca cattgaacag gcgctgactt ggaatacgcc ccatcggcgt
    14701 gaactggctg gcagatggca ccatcacgcg gatttgatca ccgcgattga cgcccaactg
    14761 tgaggcaagt tgttcgccga ggatgacatt atatttcccc ggctcgaggt cagtttgttt
    14821 cacattgacc agatacggcg ttagtggatc tttttgcgcc gggtcgatac cgagcatcac
    14881 cccgaccgcc acgctgcgcg cgctttgcag taccacatca ccggtagtaa taggtgcgac
    14941 gcgattaacg ccgtccagtt tgactgccgt ttccgggagt tgctgcgggt taagagagcc
    15001 atgctcagaa gagagaattg cctgtggcat caggccaagg atgttgtttt gcagctcgcg
    15061 ctcaaagccg ttcatcactg acaatactgt gaccagcgcc atcaccccga gggtaatgcc
    15121 gatggtagaa agccaggaga cgaaacgacc gaagcgatcc actgcacgcc cacgcatgta
    15181 acgcaggcca ataaatagag cgacaggttg gtacatgaaa tccgtctggt tgctgtagca
    15241 aagacacgag tatataagcg aaagcaagat gggtaaacgt ctgaactgta agtgatcgtc
    15301 agggctaatc tgaaaagccc ccgtaaatca atcggctaaa ttgccatgtt ttatcatcaa
    15361 tcttgccgcc tgatcttaat ctgaaaaatc atccaataca gacagatacc cattacacct
    15421 cgggtcataa ccagcaggat caaccgcatg taaattaaaa aagagcgtgt tgttctgatg
    15481 aaacaaaaag agctatggat taaccagatc aaagggttat gtatttgtct ggtggtgatt
    15541 tatcactcgg tcattacctt ttatccgcat atgaccactt ttcagcatcc gttatcggaa
    15601 gtcctgagca aatgctggat ctatttcaat ctttaccttg ccccctttcg tatgccggtt
    15661 tttttcttta tatccggcta tttgattcgc cgctatatcg acagcgtacc gtggggaaat
    15721 tgtctcgata aacggatctg gaacatcttc tgggtgctgg cgctttgggg cgtggtgcag
    15781 tggctggcgt taagtgcgct gaaccagtgg ctggcacctg agcgcgattt aagcaatgcc
    15841 tccaatgccg cttatgccga ttccaccggt gagttcctgc acgggatgat caccgccagc
    15901 accagcttgt ggtatctgta tgcgttaatt gtctatttcg tggtatgtaa aatttttagc
    15961 cgtctggcgc tgccactatt cgccttgttt gtactgctga gtgtggcggt taatttcgtt
    16021 cccacgccgt ggtggggaat gaacagtgtg atacgcaatt tgccttatta cagccttggc
    16081 gcatggtttg gcgcaacaat aatgacctgt gttaaagagg tgccgttgcg ccgccatctg
    16141 ctgatggctt ctttgcttac cgttctggcg gtcggtgcct ggttgtttac tatctcgctg
    16201 ctgttgtcgc tggtatcgat tgtggtgatc atgaagctgt tttatcagta cgagcaacga
    16261 ttcggtatgc gctccaccag cctgctgaat gtgattggct ccaacaccat tgctatctac
    16321 accacccatc gcattctggt tgaaatattc agcttaactc tgcttgcgca aatgaacgca
    16381 gcacgctggt cgccgcaagt cgaactgaca ctcctgctgg tttatccctt tgtcagtttg
    16441 ttcatctgta ctgttgcggg cttgctggta agaaaacttt cacagcgtgc attcagcgat
    16501 ctgttgttct ccccgccttc actgcccgct gccgtcagtt actctcgtta aaccatcacc
    16561 ccagccgggg cgattcaatt tgctaaacca tgtcgattac ggataatgaa cagcattgct
    16621 tatcagagat atgcccccat atgttgaggc atatcctaac gagaatctga caaccgttat
    16681 gcctgaacaa tatcgttata cgctgcccgt caaagcgggt gagcagcgtc tgctgggcga
    16741 gttaaccggc gcagcctgtg caacgctggt agcggaaatt gccgaacgtc acgccggtcc
    16801 ggtggtactc attgcaccag atatgcaaaa tgctctgcgt ttgcatgatg aaatcagcca
    16861 gttcaccgat cagatggtga tgaatctggc ggactgggaa actcttccct acgacagttt
    16921 ttcgcctcat caggacatta tctcctcgcg cctttccacc ctttaccagc taccgacgat
    16981 gcagcgtggc atactgattg ttccggtgaa tacgcttatg cagcgcgttt gcccacacag
    17041 ttttctccac ggtcatgcgc tggtgatgaa aaaaggtcag cgcctgtcac gagatgcatt
    17101 acgaacccaa ctggacagcg ccggttatcg ccatgttgac caggtgatgg agcacggcga
    17161 atacgccacg cgcggcgcgt tgctggatct cttcccgatg gggagtgagc tgccttatcg
    17221 tcttgatttc tttgatgatg aaatcgacag cctgcgggtg tttgacgtcg acagccagcg
    17281 cacgctggag gaagtagaag cgatcaatct gctgcccgcg cacgaatttc cgaccgataa
    17341 agcggcaatt gaactgttcc gcagccagtg gcgcgatacc ttcgaagtga agcgcgatcc
    17401 ggaacatatt taccagcaag tgagtaaagg cacattacct gctgggatcg agtactggca
    17461 gccattgttc ttcagcgaac cactgccgcc gctgttcagt tatttccctg ccaatacctt
    17521 gctcgtgaat actggcgatc tggaaaccag tgccgaacgt ttccaagctg acacgctggc
    17581 gcgttttgag aatcgcggcg tcgatccgat gcgcccgctg ttgccaccac aatcgctctg
    17641 gctgcgggtg gacgagctct tctcagagct gaaaaactgg ccccgagtgc agctaaaaac
    17701 tgaacattta ccgacaaaag ccgcgaatgc caatttaggt ttccagaaac tgccagacct
    17761 ggccgttcag gcgcaacaaa aagcgccgct ggatgcgctg cgtaagttcc tcgagacttt
    17821 cgacggtccg gtggtgttct cggtagaaag tgaaggtcgc cgtgaagcgc tgggtgaact
    17881 gctcgcacga attaaaattg ctccgcaacg cattatgcgt cttgatgaag ccagcgaccg
    17941 tgggcgttat ctgatgattg gcgctgccga acatggtttt gtcgataagg cgcgtaatct
    18001 ggcgctgatc tgcgaaagcg atctgctcgg tgaacgtgtt gcccgccgtc gtcaggattc
    18061 tcgccgcacc atcaaccccg atacactgat ccgtaacctt gcggagctgc atattggtca
    18121 gccggtggtc catctggagc acggcgtcgg tcgctatgcc ggaatgacca cgctggaagc
    18181 tggcggcatt actggcgagt acctgatgct cacctatgcc aacgacgcca aactgtatgt
    18241 tccggtgtcg tcactgcatc tgattagccg ttacgcaggt ggcgcggaag aaaacgcccc
    18301 gctgcataaa cttggcggcg atgcgtggtc acgcgcgcgg cagaaagcgg cggaaaaagt
    18361 gcgtgatgtg gcggcggaat tgctggatat ctacgcgcaa cgcgccgcca aagagggctt
    18421 cgcgtttaaa cacgatcgtg agcagtatca gttattctgc gacagcttcc cgtttgaaac
    18481 cacgccggat caggcgcagg ccattaatgc ggtacttagc gacatgtgtc agccgctggc
    18541 aatggatcgt ctggtgtgcg gcgatgttgg ctttggtaaa acagaagtgg cgatgcgcgc
    18601 cgctttcctg gcagtagata accacaagca ggtagcggtg ttggtgccta ccacccttct
    18661 tgcgcagcag cattacgaca acttccgcga ccgtttcgcc aactggccgg tacgtatcga
    18721 aatgatctcc cgtttccgca gcgccaaaga gcagacgcaa atccttgcgg aagtggcgga
    18781 agggaaaatc gatattctga tcggtacgca caaactgctg caaagtgacg tcaagtttaa
    18841 agatttaggc ctgctgattg tcgatgaaga acaccgcttc ggggtgcgtc ataaagagcg
    18901 cattaaagcg atgcgcgcga acgtggatat tctgacgctt actgcaacgc cgatcccacg
    18961 tacgctgaat atggcaatga gcggaatgcg tgacctgtcg attatcgcca cgccgcccgc
    19021 ccgtcgtctg gcagttaaaa cctttgtccg tgagtatgac agcctggtgg tccgggaggc
    19081 gatcctgcgt gaaattttgc gcggaggaca ggtttattat ctctacaatg atgtggaaaa
    19141 cattcagaaa gccgccgaac ggctggcaga actggtgcct gaagcacgga ttgccatcgg
    19201 tcacgggcag atgcgcgagc gcgaactgga acgggtgatg aatgatttcc atcatcaacg
    19261 tttcaacgtg ctggtttgta ccaccattat cgaaaccggg atcgacatcc cgacagccaa
    19321 caccattatc attgaacgtg cggatcactt cggtctggcg cagctgcacc agttacgcgg
    19381 tcgcgtcgga cgttcacatc atcaggcata tgcatggctg ctgacgccgc atccaaaagc
    19441 gatgactacc gatgcacaaa aacgtcttga agcgattgcc tcgctggaag atctcggggc
    19501 aggttttgcg ctggcaacgc acgatctgga gattcgcggc gcgggtgaac tgcttggcga
    19561 agaacaaagc ggttcaatgg aaaccatcgg tttctcgctg tatatggagt tgctggaaaa
    19621 cgccgtcgat gcactgaaag ccggacgcga gccgtcgctg gaagatctca ctagccagca
    19681 aacagaagtc gagctgcgga tgccgtcgct attgccagat gatttcatcc ctgacgtgaa
    19741 cacgcgtctg tcgttctaca aacgtattgc cagcgccaaa acggaaaacg aactggaaga
    19801 gatcaaagtc gagcttatcg atcgcttcgg cctgctgcca gatccggcgc gtaccctgct
    19861 ggatattgcc cgtctgcgcc agcaagcgca gaaactgggg atcaggaagc tggaaggtaa
    19921 tgagaaaggc ggggtgatcg aatttgccga gaagaatcac gttaatccgg cctggttgat
    19981 tggtttgctg caaaaacagc cgcagcatta ccgccttgat ggtccgacgc gcctgaaatt
    20041 tattcaggat ttgagtgagc ggaaaacgcg tatcgaatgg gtacgccagt ttatgcgtga
    20101 actggaagag aacgcgatcg cttaatgtaa atcgggggcg tttctgcgcc cccatctgca
    20161 acatttacaa attctttgca ctttcctgca ctatccgaca ctgtcaccat ccataattca
    20221 ggcttatctg tttattacaa taaccttata tttattatgt atttttggtg atgatcaaaa
    20281 cgcgtttttc tcgctggcta acgtttttta cgttcgccgc tgccgtggcg ctggcgctac
    20341 cggcaaaagc caacacctgg ccgctgccgc cagcgagcag tcgtctggtt ggcgaaaaca
    20401 aatttcatgt ggtggaaaat gacggtggtt ctctggaagc catcgccaaa aaatataacg
    20461 tcggctttct cgctctgtta caagctaacc ccggcgttga tccttacgta ccgcgcgcgg
    20521 gcagcgtgtt aacgatcccg ttgcaaaccc tacttccaga cgcgccgcgc gaaggcattg
    20581 tgatcaacat tgcggagctg cgtctctatt actacccgcc gggtaaaaat tcggtaaccg
    20641 tgtatccaat aggtattggt cagttaggtg gtgacacgct gacaccgaca atggtgacca
    20701 ccgtttcaga caaacgtgca aacccaacct ggacgccaac ggcaaacatc cgcgcccgtt
    20761 ataaagcaca gggaattgag ttgcctgcgg tagtgccggc tggaccggat aacccaatgg
    20821 gccatcatgc gattcgtctg gcggcctatg gcggcgttta tttgcttcac ggtacgaacg
    20881 ccgatttcgg cattggcatg cgggtaagtt ctggctgtat tcgtctgcgg gatgacgata
    20941 tcaaaacact ctttagccag gtcaccccag gcaccaaagt gaatatcatc aacactccga
    21001 taaaagtctc tgccgaacca aacggtgcgc gtctggttga agtacatcag ccgctgtcag
    21061 agaagattga tgacgatccg cagctgctgc caattacgct gaatagcgca atgcaatcat
    21121 ttaaagatgc agcacaaact gacgctgaag tgatgcaaca tgtgatggat gtccgttccg
    21181 ggatgccggt ggatgtccgc cgtcatcaag tgagcccaca aactctgtaa atcctggata
    21241 aaaaaaatcc ccgcgacacg gcatgtcgcg gggaaaaaat cagcagtagc aggcattaat
    21301 gagggttaat gcttatttat aaattactgc tgttccatgg agggtattcg gaccggttac
    21361 agaagtaata cggaaagatt ttgcgcccat ctcatccgct ttttgcgcca gctgctcttc
    21421 cagcgatccc agatttgtcc ccgcgttagc actgatagta ccgacttttt gttggccttc
    21481 tggcgttgac tgaacttcga cagccgcaaa gttggcaaat gacatggagc ttaaaatcgc
    21541 cgcagcgatg aggtttttac gtttttcata atagtggcct tatgcagatg aatgacgtcg
    21601 caagaaatta cttaacgatc gataactaaa tgatagatgt gatctggatc acatacaaga
    21661 tattttttat aacaatcatt aattaattta aaaatcttta aatttcagtc aaatagaaag
    21721 caattatttt tatgggatct ggactggtga acggaggtga ttatttttat aataaccttt
    21781 cattcacagt caccaggtac aacggcatca tggcaactga ctcaacacaa tgtgtaaaaa
    21841 aaagccgtgg ccgcccaaaa gtgttcgaca gggatgccgc gcttgataag gccatgaaat
    21901 tgttctggca acacggttat gaagcgactt ctcttgcgga cctcgtcgaa gcaaccggag
    21961 ccaaagcgcc cacgctatac gcggaattta ccaacaaaga ggggttattt cgtgccgttc
    22021 tcgaccgcta tatcgatcgt tttgccgcta agcatgaagc acagctgttt tgtgaagaga
    22081 aaagcgtgga gtctgcgctg gctgactatt ttgcggccat cgccaactgc tttaccagca
    22141 aagacacccc ggcaggctgc ttcatgatca acaactgcac ccccctctcc ccagattcag
    22201 gagatatagc caatacgttg aaatcacgcc atgcgatgca agagcgcact ttgcagcagt
    22261 ttttatgtca acgacaagcg cgcggggaaa tcccgaccca ctgtgacgtg acacatctgg
    22321 cagaattcct taattgtatt attcagggga tgtcgatcag cgcacgcgaa ggtgcatcgc
    22381 tggaaaaact gatgcagatt gccggaacga ctttgcgttt atggcccgaa ctggtgaaat
    22441 aagtcgcaaa aaaggcccct cagcctgagc gaatgagggg ccaaattgca gagtacagcc
    22501 ttgttatact ttgttattca aaatcagttg cccgttacta tctagcggga tctgcgtacc
    22561 cggatcgcgg tccatgcgga ttttgccctg ctgatcgcca atcttatagg tcacatcata
    22621 accgagcatt ttttctgact tgtcatacac cgttttacaa cgctgttgcg tagtcgtgta
    22681 agtatcgctt tcctggagag agccctggat ctggttaccg gcatatccac cacccagcgc
    22741 ccccacaaca gtggcgacat ctttaccgcg accaccacca aactgatgcc ctatcacgcc
    22801 accagcaaca gcgccgagca ccgacccggt aatgcgattt tcatcctgca ccggtcgacg
    22861 atgggtcact gtgacgttgc gacactcctg acgcggtgtt ttaaccgttt ccttgattgg
    22921 ggttgcagaa acaacctgag cgtattgcgg gccccgttca aacacgttca gactggccac
    22981 tgccgctacg cccaacgcag ctgcgacacc aatcccgata cccgccaaca ttgatttatt
    23041 cacgggaaca cctccttctt tgcagttatg ccaatcatgc aagcgaagga gaaaagcagc
    23101 tatcagacag aagatgaaaa gcgcgggaat gactggaaat acttaaggat tcggaggagt
    23161 ctgaaggaat gcctgatgcg acgcttaacg cgtcttatca ggcctaccgc tcgtaccatt
    23221 tgtccagggc ggataaggcg tttataccac atccgccagt gtacgtcgat taatgcaact
    23281 tcaaacgcgg acggataacg cggttaatac tccccaccag catcattaat ccggttttaa
    23341 agtaaccatg cagcgcaatc tgatgcattc ggtatagcga gatatataca aagcgcgcaa
    23401 ttcgtccttc aatcatcatt gagccgcgcg tcaggttacc catcaggcta ccaacggtgg
    23461 agaagttcga cagcgatacc agcgaaccat gatctttata ctgataattt ttcagcggct
    23521 taccgttcat ctgcgccaga atgttgttca ttgcgcaagt cgccatctgg tgtgcagcct
    23581 gagcacgcgg cggaacaaag cccccttccg gacgcgggca tgacgcgcag tcgccaatag
    23641 cgtaaatgtc tggatcgcga gtggtttgca gcgtcggttc caccaccagc tggttgatac
    23701 ggttagtttc aagaccaccg atatctttca ggaagtctgg cgctttgatc ccggctgccc
    23761 ataccatcag atcagcctca atatattcgc catctttagt gtgcaggccg ccttcatcag
    23821 cactggtgac catggtttgc gtcagcacgc gaacgccaag tttcgttagc tcgttgtggg
    23881 ccgcagtaga gatacgtggc ggtaatgcag gcaaaatacg ttctcccgct tctaccagcg
    23941 ttacgttcag ggcttcgttg gtcaggcctt tgtaaccgta gctgtgcagt tgcttgatcg
    24001 cgttgtgcaa ttcagcggag agttctacac ccgtcgcgcc gccgccgaca atcgcaatgt
    24061 tcactttgcc atttgcgccc aggttggcag agtatttcag gaacagattc agcatctcct
    24121 ggtggaagcg acgcgcctgg tgcgggttat cgaggaaaat gcagttctct ttgacgcctg
    24181 gcgtattgaa atcgttagag gtgctaccca gcgccatgac cagggtgtca taggcgattt
    24241 tacgttccgg aaccaacagt tcacctttct cgtcgcgtag ttctgcaata gtgattgttt
    24301 tcgcttcacg atcaatatca atgacggaac ccaactggaa ctggaaacca tgattgcgcg
    24361 catgggccag atagctcaac gcatcgacgc cttcatcaag cgagccagtc gccacttcgt
    24421 gcagcagtgg tttccacagg tggctgtggt tacgatcgac cagcgtaatt ttggcttttt
    24481 tcttacgtcc cagcttatgc cccagctgtg ttgccatttc cagcccacca gcaccgccgc
    24541 cgacaatcac aatctttttc aatggcgtag tcaacgtgac ccccttaaat ttattaacca
    24601 attgttaatt aatggttatt aacatagctc ttaattaaca acaggttaca gcaatgaaaa
    24661 tgttcttcgc tattgagaat aacatgaatg gtgcattggt cataccaaaa ttgatgttaa
    24721 tcaagttttg ttgcttaaaa gataggtaac tttaccgggg gagatcaaaa aatagagttt
    24781 attgtttatt atttgagcaa attcggaagg gtaatatagg gtgaagcgta tatcgagggc
    24841 gtttggtcgt cctgataaga tgcgcagcat cgcatcaggc attgatgctc gactgtcgga
    24901 tgcggcgtgg acgccttatc cgacttacag aattcggagc gcatcaggta ccggcaaggt
    24961 tacctgatgc gacgagggca tttacccgag ggttttgaac gctttaatgc gctgtaaatg
    25021 cggggagata ttcttgaatt tgtgcgtctg ctcttcgtcc cagacaatct cgtaataatg
    25081 atgcaactct tcagatgtcc gctggctgtt aagtgcttca tcattacgcg acaaaataac
    25141 cagacaacga tcgcgattct tctcgcggaa gttggtcaca cacttagtgg caatatcggc
    25201 atactcttcc gggcgatcaa tcttgccttc catgttctca taagggaaca aattagggtt
    25261 gaagatcacc tggcggatat cgcaaagaaa accaatccgt tccgcccagt atccgcccag
    25321 gccaacgccg caaattagcg gacgctcgtc aacgttcagt tgcaacattt tgtccacttc
    25381 tttaagcaga tgctgcatat catgtttcgg atgccgcgta ctgtagctta tcaagcgtac
    25441 atccgggtca ataaactgca attgtaagac tttctcgtgg ttacccggac tgttagagtc
    25501 aaaaccgtgt aaatagataa tcatcgcttc ctcacataag ccagggttag tgacctgctt
    25561 tctcttcctg ccagcgttca tgtaactgat tcagacgggt gctgatcgct ttccagcgag
    25621 ccgagtccat cagttcctgt cgcgaaaatg aacctttatg atacaaacgt gtaacacgtt
    25681 ctgccttgat cggtgacaga ttatctaaca cgctgacggc ccctttacga ttattgcaga
    25741 ccaggatcat atcgcaaccc gcatccagtg atgcctgccc gcgttcggca taactgccca
    25801 taatcgcggc accttccatc gataaatcgt cagagaaaat cacgccgtca aaacccagtt
    25861 cctgacgcaa aacggttttc agccagtagg gagaaccgct cgccggacgc ggatcaacat
    25921 cactgtagat cacatgcgca ggcataatgg cgtcgagttt attttcgcgg attaaggaac
    25981 tgaagaccga catatcttta gcgcgaatct ccgcttgtgg acgtggatcg cacggtgttt
    26041 ctttgtgtga gtctgccgtt actgcaccgt gtcctgggaa gtgtttcccg gtcgttttca
    26101 ttccggcttc atgcatacca tcaataaacc ggctggcaat tgccagggct ttttgtggat
    26161 cggcatgata agaacgctcg ccaatcgccg cgctgatatg cccgacatcc agcactggcg
    26221 caaagctgat atcaatatcc atagcgatca tttcgctggc catcagccaa cccgcctctt
    26281 gcgccagttt gccgccctct tccattccca acagcgcagc aaaggattgt gctgccggta
    26341 agcgggtaaa accttcgcga aaacgctgca cgcgtccacc ttcctgatct accgccacca
    26401 ccagatgatt gcgcgatgct gcgcggatct ggcgcaccag ttcacgtaac tgggcaggat
    26461 catgatagtt acgcgtaaag agaatcagcc ctcccaccag cggatgcgcc agtatttcac
    26521 gctcttccgc gtccagttcg taaccttcga catccaacat tactggaccc acactgctct
    26581 ccttattgtt ttattaatag ctgccgccag gtgtcatcgg ccagcctgat aaattgttga
    26641 tcgccggttt gtcgccagcg gtactcaaac caccctcctt tgagcatcag cagccaggga
    26701 aaccatcgcc tgacctgacg ccataattgc gccggataaa tcttcgcgcg agtggcatag
    26761 tcattgacca attgccggtg ctgttcagta ttttccaccc acaccgccgc cagttccagc
    26821 gcgatatcac catctccagc atactcccag tcgatgagtt ttaaccctga tgcgctatgc
    26881 actaaatttc cggcgtggac atccatatgc aatggactta agcgtaaagg tcgtggttcc
    26941 cgcgctttgc gcagacgttt taacattcgc agccaaccca cagtccgccg cgccggatcg
    27001 ctttgctgcc agtacagttc cagtaagggc aacagcgtta ttcgccagcc aaaacgtggt
    27061 tgttgatgta gataatacag caagcctgcc agttcgttgg tatccggcaa atacgttttt
    27121 accgcgccgg gcagatagtc gactaccatc cagtcacgga gatataaatg cggcttcggt
    27181 gcaatgcttg cgggtagttg tgataaagcc cgatactggc gcaagaacgc ggactgcggc
    27241 gcatcaggat cgtgcggctg acgcacaaca aaacgctggc cctgatgttc aatgagaaaa
    27301 ctaccgccac taagcccact attaaacgtc gtgacgggat gaaactgcgg gaaaaagcgc
    27361 gacagcaatt cgtcgcgcgt gatgggatta ttgctgcgaa acggcacctt tacctgacca
    27421 gataatttcg cccgtctgca ccagcatcag ctgcatttgt agggtcggag cgttaacgtt
    27481 gccagaggcg ctggagtaca gcacgtaatg agcgccgaca ttgcgggcaa tgcctatggc
    27541 tttactacgg gtgcctaaac tgtcctgcgg cgacaaacct aactgttgct tagccatcga
    27601 cagttgctgg gcggaaacca gggtaaattt cccgttatta gccagcgcat ttcgcagcgt
    27661 ttcggtcgct tctgcggcat tcagcgaacc gttagtacgg ttgttaacgc tatcaaccag
    27721 caggacgcta cccgcagtca ccccgtcagc cccaagcatc ttactgacca tcggctgcat
    27781 tgcgccattc cagtcataat ggcgaatatg cggcgcaggc ggtgccgttc gatcttcgtg
    27841 ctcaattggg cctggctgct gcgggatcgt cggcaccgag ggcactgtgg ggacaggctg
    27901 ttgtggctcg gctggttgtt ccggcgctgg tttcacttct tctaccggtg caggttcacg
    27961 ttgccccaca cacccggcga gaaacatcgc cagcgcggta atcaaggcgt agcgactcat
    28021 ttttgtcatc aagattcacc cctttacaaa tatagataaa gtctgacttt gtgcgccccc
    28081 agaaaattgg cgctgccgta cagcgttacc gccgaatgtg cgggaatggt aacgctgcgt
    28141 ggcctttcca gaggatgcat ctccagccct ctggcgtcat accagtaaaa acgataatga
    28201 acggtaacgg gttcttgcct ttcgttataa agcgttgagg atgctgaagg ttgaatatca
    28261 gacgtcgaaa ggacgggctt ttctgcactg atgcccgcag ccaataaaga tgactccatc
    28321 accagcgatt gctcatcatt caccggaatt tccggatgtg aacgacagcc caccagcagt
    28381 aacaacacca gagacaccag cccaaagcat ccttttctca tcgttacaga cctttatgcg
    28441 ccagcattgg tcccagcgga cggccaccca acaagtgcat atggatgtgg taaacctctt
    28501 gtccgccatg gcggttggta ttcatgatca gacgatagcc atcttcggca ataccttctt
    28561 gctcagcaat ttttgccgct acggtgatca tgcgtcccag cgcctgctca tgctcagctg
    28621 acacgtcgtt cacagtcgga atgagaatat tcggaatgat cagaatatgc gttggcgctt
    28681 gcggcgaaat atcgcgaaac gccgttacca gatcatcctg gtagacgata tcggagggga
    28741 tctcacgacg aataattttg ctgaatatag tttcttctgc cacgacattt tcctttttca
    28801 aatagccatg cgttgcgcca tgctacgccg cgccacactg cgagtataga gtatggtcga
    28861 gttaaacctg ctctttcaat cttaactcac gatttcttaa gcaaaaaatc gttttgctgc
    28921 cgtatatatc gccattattc ccatttctgc ctgcgaatgc gctgaaaaga cattctctgt
    28981 ttggtttaca cgacaaatat gaatgcgtat atttctcatt tgcatttgca tttgcagcaa
    29041 aattattcgc acactaaata taaccgctca tcaccctcct ccggcatgag cctgacgaca
    29101 acataaacca agagatttca aatgctttca acacaattta acagggataa tcaatatcaa
    29161 gtcatcacca aaccgtcact acttgccggt tgcatagcac tggcactatt accttctgcc
    29221 gcttttgctg caccagccac tgaagaaacg gtgattgttg agggttcagc cacagctcca
    29281 gatgatggcg aaaatgatta cagcgtaacg tctacctctg cgggtaccaa aatgcagatg
    29341 actcaacgtg atattcctca gtcggtcact attgttagcc agcagcggat ggaagatcag
    29401 cagttacaaa cgctgggcga agtgatggaa aacacgctgg ggatcagcaa aagtcaggcg
    29461 gattccgatc gtgctcttta ttattcccgc ggattccaga tcgataacta tatggttgat
    29521 ggtatcccca cctattttga atcgcgctgg aatctgggcg acgcactttc tgatatggca
    29581 ctgtttgaac gcgtagaagt agtgcgtggc gcgacaggac tcatgaccgg gacgggtaat
    29641 ccatctgcgg caattaatat ggttcgaaaa cacgcgacct gtcgtgaatt taaaggcgat
    29701 gtctcggcgg aatacggtag ctggaacaaa gaacggtatg tggcggattt acaaagccca
    29761 ctcaccgaag acggtaaaat ccgcgcgcga attgtcggcg gctaccagaa taacgactca
    29821 tggctggacc gctacaatag tgaaaagacc ttcttctcgg gcattgtcga tgctgattta
    29881 ggcgatctta cgacgctttc agccggttac gaatatcagc gcattgatgt taatagccca
    29941 acctggggcg gtttaccgcg ctggaatact gatggcagca gcaacagtta cgatcgcgca
    30001 cgcagtaccg cacctgactg ggcgtacaac gataaagaga tcaacaaggt ctttatgacc
    30061 ctgaagcagc ggtttgctga tacctggcaa gcgacactga atgccaccca ctctgaagtc
    30121 gaatttgaca gcaaaatgat gtatgtcgat gcctatgtaa acaaagcgga tggtatgctg
    30181 gttgggccat acagtaatta tggacctggc tttgatcatg tcggcggcac cggttggaac
    30241 agtggcaaac gtaaagttga tgcgctggat ttgttcgctg acggtagtta tgaattgttt
    30301 ggtcgtcagc acaatctaat gtttggtggc agttacagca aacaaaacaa tcgttcagtt
    30361 catgggccaa catcttcccg gatgaaattg gcagtttcta caactttaat ggcaatttcc
    30421 cacaagccga ctggtcatca cagagcctgg cgcaggacga taccacacat atgaaatcgt
    30481 tatatgctgc cactcgtgtc acccttgccg atccgctgca tctgatcctc ggcgcacgtt
    30541 ataccaactg gcgggttgat acgctgactt acagcatgga gaaaaaccac accacgcctt
    30601 acgctggtct ggtgtttgac atcaatgaca actggtcgac ctacgccagc tatacctcta
    30661 ttttccagcc gcaaaatgat cgtgacagtt caggcaaata tctggctcca atcaccggta
    30721 acaactacga gctgggtctg aaatcggact ggatgaatag ccgtctgacc accacgttag
    30781 ccatcttccg tattgagcag gataatgtcg ctcagtccac cggtacacct atccccggca
    30841 gcaacggcga aaccgcctat aaagcggtgg atgggacagt cagtaaaggg gtggaatttg
    30901 aactcaacgg cgcaattacc gacaactggc agctgacatt tggcgcaacg cgctatcttg
    30961 cagaggataa cgaaggaaac gccgttaatc ctaatctgcc acgcaccacg gttaaaatgt
    31021 tcaccagcta tcggttgcct gtcatgccag agttgacagt cggcggtggt gttaactggc
    31081 aaaatcgcgt gtataccgac accgtgacac cgtatggcac cttccgcgcc gagcaaggta
    31141 gctacgcgct ggtggatctc ttcacccgct accaggtgac gaaaaacttc tcgttacagg
    31201 ggaacgtcaa taacctgttc gacaaaacct acgataccaa catggaaggt tctatcgtct
    31261 acggcgcacc gcgtaatttc agcattaccg gcacgtatca attctgaata acacctgtaa
    31321 aaaaggcagc catctggctg ccttagtctc cccaacgtct tacggattag tggttacgga
    31381 tgtactcatc catctcggtt ttcaggttat cggatttagt accgaaaatc gcctgaacac
    31441 cagaaccagc aaccactacg cccgctgcgc ccagtttctt caggccagcc tgatccactt
    31501 tagacacatc agcaacgctg acgcgcagac gggtaataca tgcgtcgagg ttagtaatgt
    31561 tttctttacc accaaatgca gcaaccagag ccggtgccat ttcgctggta cctgttgctt
    31621 ttgcatcttc agtcgcgtct tcacgacccg gcgttttcag atccagtgct ttaatcagca
    31681 cgcggaagat ggtgtagtaa acaatcgcat aaccgatacc gacgatcggt aacagccaca
    31741 gtttgctgct gttaccagac agaacgatga agtcgatcag accgtgcgag aacgacgtac
    31801 cgtcacgcat ccccagaaga atacagattg ggaatgccag gcctgccaga atcgcgtgga
    31861 tgatgtacag gatcggcgca acgaacatga agaagaactc gatcggctcg gtgataccgg
    31921 tcaggaacga ggtcagcgcc gcggagatca taataccgcc cactttcgca cggttttctg
    31981 gtttagcaga gtgccagata gcaattgcgg cagctggcag accgtacatt ttgaacagga
    32041 agccaccaga cagtttaccc gcagtcgggt cacccgccat ataacgcgga atgtcgccgt
    32101 ggaaaacctg acctgctgcg ttggtgtatt caccaatctg catctggaaa ggtacgttcc
    32161 agatgtggtg cagaccaaac ggtaccaggc aacgttcgat gaaaccgtaa atgccaaacg
    32221 caactaccgg gttctggtaa gcagcccact gagagaaggt ctggattgca gaaccaatcg
    32281 gcggccaaat gaaggacagc acaacgccag taaagatggc agccaggcca gaaatgatcg
    32341 gcacaaagcg tttaccggca aagaagccaa gatactcagg cagcttaata cggtagaaac
    32401 ggttaaacat gtacgctgca atcgcaccgg agataatccc tccgagtacg ccagtatccg
    32461 ccaagtgttt agaggcgatt tcttcagcag gtaaatgcag taccagtggc gcaaccacgg
    32521 ccatggtttt aaccatgatg ccataggcaa caactgcggc cagcgcggat acgccatcgt
    32581 tattggtaaa gccgagggcg acaccgatcg caaaaatcag tggcatgttt gcaaagacgg
    32641 aaccgcctgc ttctgccata acatgcgata caacggcggg cagccagctg aaattcgcgg
    32701 aaccgacgcc cagcagaata cctgcgatag gtagtacgga taccggcagc atcagcgatt
    32761 taccgacctt ttgcaggtta gcaaatgcat tcttaaacat aattgagagt gctcctgagt
    32821 atgggtgctt tttttacgtt ctcacgcgtg gcaagggggg agagcctcgc cgtgtacagg
    32881 gcatctaagc gccctttatt tattacacag agtaaaataa ttcagtgcca atttgtttga
    32941 cggctatcac gtttcaactt cagatcattg gagaagttgc acattttgac aaaacatatg
    33001 tcacaatatt tcaaacgcgg ttattcaccg cccaattttc ggcggtgaac tttactcgtt
    33061 ttagccatta attacgagct ttaaaaaaac tcattcaacg gatggattga aggcgggaag
    33121 cgtcgatgtg aaacagacgg gcgaagttat cggtggttac ctgcgccagt tcttcaacgg
    33181 caacaccttt caacacagcc atgtattctg caacgtcacg aaccatcgca ggttgattct
    33241 cttttcctcg atgcggtacc ggcgcaaggt aaggtgagtc agtttccacc agtaatcgat
    33301 ccagggggac ataacgcgca gcatcgcgca gttgctccgc attacggaag gtcacaatgc
    33361 cggaaaagga gatgtaaaat ccgagatcca gtaatttacc cgccgtttct ctgtcctctg
    33421 taaaacagtg tagtacgcca ccgcaatccg tcactttttc ttcgcgcaga attgccagcg
    33481 tatcggcacg ggcgtcacgg gtatggacga taaccggctt gttcagttca cgaccaatct
    33541 ggatatgatg gatgaaggac tcttgctgac gtactttagt ttccggtgtg taataataat
    33601 ccagcccggt ttcacccagc gctacaacac cctcttctgc cgccagacgg cgtaaatctt
    33661 ctacatcgta gggatcattc tggttgagtg gatgcacgcc acaagaaaat acgacgttgt
    33721 cacgttcgcc taccagatcc cgcatatgta aataacccgg taatgttgtg gcgactgcca
    33781 gacaaaattt cacatcgcgt gcggcggctt tcgccagaac gtcatccacg tccttatgca
    33841 aagattcata atccagacca tcgagatggc agtgtgagtc gactaaaaac atgatgtctc
    33901 tcttaaagat gaggaaccgg tagcacaacg cccggttgca ggtaatgctc aatacgcagt
    33961 aaaagatcgg tgatgagaag ctcgcggttg atgcctgtga cagacattaa ctgttcacga
    34021 atgtggcaaa catcccccag tatagcctgc aggcgcgagg gagaaagatg gtttgccagt
    34081 tcggcgacca gtcccggcac atcaacattg gtcacctgcg cagcgccatg atggcgtttt
    34141 agcgcatcca tcagcaacgt tgccagccag tgtaaacgcg ccggagcttg ttcatgatta
    34201 agggccgcca gcagcgaata ccagtcgccc gatggcacgc tatatgccaa cgcctgacac
    34261 aatgtttcac gagcctgcca gttatctccc tgaaacaacg ccagtgccgc gccaggcgaa
    34321 ccggcgctta agcgcaatgc ggcaagtaat gcatcctgtg acattgtcac ttcgcgtgaa
    34381 agccaagtca cggcgtactg ttctggaggc ggcgcaaggt aatgtaaccg acaacgacta
    34441 cgtaatgttg ccagtaaacg ttcaggctcg cgggtagcca ggaaaaacca ggtttctgct
    34501 ggtggctctt caagcgtttt cagcaatgcg ttagccgcgg cgtcggtcag taaggcagca
    34561 tcggttaccc agacaacttt cgcaccacct aggcgtgcgt gctcattcag cttttcggtg
    34621 acctcacgta ccgcatcaat gcccagcgta tttttccctt tttcgggagc cagggtgtag
    34681 taatcgggat gcgtgccagc ctgcatcaac tgacatccac gacagtgacc gcaacttttg
    34741 tggccctgcg gttgttggca gagtaaataa cggctcaggg cgtagattaa agcatcatcg
    34801 cccatgcccg gtaacgcctg aatgagtagc gcatggtgac ctcttccggc ctgatagctg
    34861 gctaccagtt tttcgaaatc aggtcgtaac catggatacc atctcatgcg tccaactcct
    34921 tcacccagtg ggtcacggta gtgcggattg catccatcac ggcctccagc ggctgggtgg
    34981 catcaatggt atgaatactt ttatcttgtg cagccagttc cagatagcgg gcgcgggtgc
    35041 gattaaagaa atcgaaagac tcttgctcaa tacgatccag ctcgccgcgc gcacgcgcgc
    35101 gttttaagcc aacttccggg gtaacatcga gatagagcgt taagtctggg cgaaaatccc
    35161 cgagaacagc atcacgcagt gttgccagca tatgttggtc aataccacgt cctccgccct
    35221 gatacgcctg agtggaaaga tcgtggcgat cgccaatcac ccaggtgccg ttagccagcg
    35281 caggtttgat gaccgtttct accagttgaa cgcgcgcggc ataaaacatc agaacttcgg
    35341 ctttatcggt aatgacttca tcgcctaccg atttgatatc cagcaccagg cttcttaact
    35401 tttcggcaag ttgcgtaccg ccaggttccc gagtgaaaac catgtcgcgg atacccagtt
    35461 gctcgagcgt ctcaaccacc acattacgcg cggtagtttt gcctgcgcct tccagcccct
    35521 caatgacgat atacttactg cgcatttttt tccttaagca ctttcagata atcctgcaca
    35581 gacttgttat gactggcaag attggtatta aacgtgtgac cacctttacc atcggccaca
    35641 aaatagagat acggcgtttt tgccggatgc gcagcagcct tcagcgaatc cgcccccggc
    35701 gtagctatcg cacccggcgg caaaccggta atggtatagg tgttatacgc tgtcggcgtt
    35761 tccaggtctg cacgagaaag tttgccatta taacgctctc ccatcccgta aatcacggtt
    35821 gggtcggtct gcaggcgcat accaatgcgt aaacggttga taaataccga ggcaacctga
    35881 tcgcgttcac tggcaacggc ggtttctttt tcgataattg atgccatcgt caccagctga
    35941 tttttatctt tataaggcag accgtccgca cgcccttccc aggcgctatc gaccgctttc
    36001 accattttct tgtgaactcg cttgagtaac gcgacatcgg tggtattggc ggtatacatc
    36061 aaggtgtctg gccagaacca accttcaatc cactccgggt tttccagttc aagtgcctgc
    36121 gctacggtgg cgtacttatc gtcactcagc gtgtgcttga tatacggagc ctcacgcaat
    36181 tgcttgaggt agtcgctcag acgcatccct tctaccagtc gcagcgggaa ctgtgcttct
    36241 ttaccgcttt ccaacaattt cagcatctcg cgcacggtca tctgcggtgt aaagcggtaa
    36301 gtcccggctt taaagtgaga aagatccggt tcgatacgca gcagccattg aaaaacccgt
    36361 gggcgattga tgatcttatc agcataaagc tgttcaccga gcgccagacg tccggtccct
    36421 ggcttcaggg taaatatcgt ctcttctttg ataagcaatt tgctgtcggc aagatggcga
    36481 accttccaga cgcccacacc agcggcgata cccagtacca ccagcaataa caagataatc
    36541 aataacactt ttttcatgac taattcgggc gctcacaaag tggggctaaa tattcatata
    36601 acgttgctga cgaaaaggtg acatcgccac aggcacgtac gggcatcact ggcattaacg
    36661 cattacaaat aaccatctca tctgcctgca acgcctcctc cagcgaggct tgcacttcga
    36721 caagctgata agaggattgt gccagtaaac ggatacagaa ttgtcgcata atgccgttaa
    36781 cacctgcctg atccagtcgc ggcgtataaa ctacattgcc cttccgccag aacaaattag
    36841 ccgcacagca ttccgtaacc cacccttcgc tgtcaaggac cagcgcctca tcagcgtttg
    36901 tctgctcaag atgagagcga atcaatactt gctcaagccg attaagatgt ttaatacctg
    36961 caagatgagg attgcgcccc agccgcaccg ggcttagcgc caacgtcatc ccttcgttac
    37021 gcaaacggtc gtaatgtgca ggataagccg taacggagag aatccgcgtt gctggtccgc
    37081 tgttcaatgt gctgtaccct cgcccgccac taccgcgact gatcacgact ttcagcacac
    37141 cattttgctg ttctgctgcc agcgttttca tctcttgttc aagctgaggc cagaagtcac
    37201 aggaaatcat caaccgctga caagcatcct gtagtcgcag gatatgcgcc gataacaaac
    37261 tgactttacc gtcgataact ctggcggtgg taaaacaacc atcaccaaac tgcgttgccc
    37321 gatcgcttac tgccagcgat tcctgcttat aaccgttaat taagaacata ccggctcctt
    37381 atcagagaat tgttagtgtg gcagcatgtt cactacggaa caagtcggaa taaaagctaa
    37441 gaaaaaaggc ccgcaagcgg accttttata tgggtggaaa atgacaactt agatcttttt
    37501 aaagatcaaa gaaccattag tgccaccgaa gccgaaggag ttacacagcg tgtattccat
    37561 tccgctgacc tgacgtgctt catgcggtac gaaatccaga tcgcaacctt catccgggtt
    37621 atccaggttg atggttggcg gaacagcctg atcgcgcagc gccaggatgg agtagataga
    37681 ttctactgca cccgccgcac ctaacaggtg accggtcata gatttcgtgg agcttaccaa
    37741 cacacggctt gcagcttcac cgaagatggt tttcaccgcc tgcgcttcag ctttatcgcc
    37801 agccggcgta gaagtaccgt gcgcgttaac gtagccaatc tgactcgctt caatgcctgc
    37861 atcacgcaga gcatttgcca ttgccagagc tgcgcctgcg ccattttctg gcggtgacgt
    37921 catatgataa gcatcgctgc tcataccaaa gccgacgagt tcagcgtaaa ttttcgcacc
    37981 gcgttttttc gcgtgttcgt actcttcaag taccagcata ccggcaccat cgcccagtac
    38041 gaagccatca cgctctttat cccacgggcg gctcgccgct tgcgggttat cattgcgggt
    38101 agataatgca cgtgccgcgc caaaaccacc aacgcccagc ggcgtactgg ctttctctgc
    38161 gccacctgca accatcacgt cagcatcgcc atacgcgata atacgcgcag catggccaat
    38221 gttgtgcacg ccggaagtac aggcggtcgc gatagagatg cttgggccac gcaggccata
    38281 catgatagtc agatgacctg ccaccatgtt cacaatcgtt gacggaacga agaatgggct
    38341 gatcttacgt ggaccaccgt tcatcagaga tgtgtggttt tcttcgatca gtccgaggcc
    38401 gccaatcccg gagccaattg cggcaccaat gcgggttgcg ttctcttccg ttatttcaag
    38461 gccagaatcc tgcatggcct gaacgccagc gacaattcca tattgaatga aggcatccat
    38521 cttgcgctgt tctttgcgcg agataatgtc ctcacagtta aaatccttta ctaagccagc
    38581 aaatttcgtt gcataggcgc tagtatcgaa atggtcgatt aggctgatgc cactctgacc
    38641 ggcaagcaga gctttccagg tagactctac ggtattgccg acaggagaca acatgcccag
    38701 tccggtcaca actacacgac gcttagacac gtttgtcctc caaggaggga aaaatgattc
    38761 tagtgggaca aaaagataaa actcaggcgg tcgaacgacc gcctggagat gttcacttac
    38821 gcctggtggc cgttgatgta atcaatggca gcctgaacgg tggtgatttt ctcagcttct
    38881 tcgtccggaa tctcagtatc aaactcttct tccagagcca ttaccagctc aacggtgtca
    38941 agagaatccg cgcccaggtc ttcaacgaaa gaagcattgt tggtaacttc ttcctgctta
    39001 acgcccagct gttcgccgat aattttctta acgcgttctt cgatagtgct catactctta
    39061 aatttcctat caaaactcgc tttcgcgatg gttttcgtag tgtataaaat gttgaaaaat
    39121 ttgcaactaa atcccggcag gtcttaccac gattttacgt tattttgagg ccttaccccc
    39181 caataacgca aatcattttg cactaattat tactcataac cacgcagact gcgcgcaaca
    39241 tgagcatttt gtgcaaatcg cggtcagacc atgtacatcc cgccgttcac atgcaaagtt
    39301 tcacccgtga tgtaagctgc ttcgtcggat gccaggaatg caaccgcgtt ggcgatttcc
    39361 tgtgcgccgc cgaggcgacc cgcaggaacc tgcgccagga tacccgcacg ctggtcatcg
    39421 ctcagcgcac gtgtcatgtc cgtttcaata aagcccggag caacaacgtt tacagtaata
    39481 ccgcgtgacg caacttcgcg cgccagtgat ttactgaagc cgatcaagcc cgctttcgcc
    39541 gcagcgtagt tggcctgacc gccatttccc atggtaccaa ccacagaacc gatagtgata
    39601 atacgaccat gacgcttttt catcatagcg cgcattaccg cttttgacag acggaaaaca
    39661 gatgaaaggt tagtttcgat aatatcgttc cactcttcat ctttcattcg cattaacagg
    39721 ttatcacgag tgataccggc attattgacc aggatatcca cttcaccaaa ttctgcgcga
    39781 attttttcca gaacagattc gatagatgcc gggtcggtca cattcaacat cagacctttg
    39841 ccgttggcac ctaaataatc actgatcgcc tgagcgccat tttcactggt cgcagtgcca
    39901 ataactttcg cgccacgggc tgcgagcgtt tcagcaattg cgcggccaat tccgcggctt
    39961 gcaccggtta ccagtgcgat ttttccttca aaattcatga ttttcctctt ttaaagctcg
    40021 agcgccgctg ccatcgctga aggttcgttc agcgccgagg cggtcagggt gtcgacaatg
    40081 cgtttcgtca ggccagtaag caccttgccc gggccgactt catagagatg ttctacgcct
    40141 tgcgctgcca tgtactcaac agacttcgtc cactgaaccg ggttatacaa ctgacgtacc
    40201 agtgcgtcac ggatggcatc accattggtt tcgcatttca catcaacgtt attcacaaca
    40261 gaaactgttg gtgcgttaaa ggtgattttc gctaattcta ctgccagttt gtcggctgct
    40321 ggtttcatca gcgcacagtg agacggtacg ctcactggta acggcagcgc gcgttttgcg
    40381 ccagcagctt tacaggcagc gccagcacgc tcaaccgctt ctttatgacc ggcaataacc
    40441 acctgtcccg gagagttaaa gtttaccgga gaaacgacct gaccttctgc agcttcttca
    40501 cacgctttcg caataggcgc atcatccaga ccgatgattg ccgccatagc gcccgtgcct
    40561 tccggtacgg cttcttgcat gaacttaccg cgcatctcaa ccagacgcac cgcatcagcg
    40621 aaatcaatca cgccagcgca aaccagcgcg gagtattccc ccaggctgtg accggccatc
    40681 attgccggtg ctttaccgcc ctgctgctgc catacgcgat acagcgcaac agatgcagtc
    40741 aacagcgctg gctgggtttg ccaggtttta ttcagttctt cagctggccc ctgctgggtc
    40801 agcgcccaca ggtcgtagcc cagcgccgca gaagcttcag caaacgtttc ttcgacaatt
    40861 ggatagctcg ccgccatatc agccagcatt ccaacggttt gagaaccctg tccagggaac
    40921 acaaatgcaa attgcgtcat gttttaatcc ttatcctaga aacgaaccag cgcagagccc
    40981 caagtgaatc caccgccaaa ggcttcaagc agaaccaact gccccggctt aatgcgcccg
    41041 tcgcgtacag cttcatccag cgcgcacggg acagaggccg cagaggtatt accgtggcga
    41101 tccagcgtca ccacgacatt gtccatcgac ataccgagtt ttttcgccgt tgcactgata
    41161 atacgcaggt tagcctgatg cggaaccagc cagtccagtt gagaacggtc aagattattc
    41221 gccgccagcg tctcatcaac gatgtgcgcc agttccgtta ccgcaacctt gaagacttcg
    41281 ttgcccgcca tcgtcagatg aattgaattc tctggattca cgcggtcggc gtttggcagc
    41341 gtcagcaatt caccataact accgtcggca tgcagatggg tggaaatgat tcccggctct
    41401 tcagaggcag ccagcaccgc agcgcccgcg ccatcgccaa aaataataat agtcccacga
    41461 tctgttggat cgcaggtgcg cgccagtaca tcggaaccga cgaccagagc atacttcacc
    41521 gccccagatt tcacgtattg atcggctacg cttaatgcat aggtgaaacc tgcacaggct
    41581 gctgcaacgt caaatgccgg gcaaccttta atgtccaaca tgctttgaat ctgacaagct
    41641 gcgctcggga aagcgtgcgt agcagaagtc gttgccacaa cgatcaggcc aatctggtct
    41701 ttctcaatgc ccgccatctc aattgcgcgt gtcgccgctt caaagcccat ggttgaaacg
    41761 gtttcgtttg gcgcggcaat gtggcgttcg cggataccgg tacgagtgac aatccactcg
    41821 tcagaggtgt ccaccatttt ttccaaatcg gcgtttgtcc gcacttgttc gggcagatag
    41881 ctgccagtac caataatctt cgtatacatg tacgctcagt cacttttcgg ttatataccg
    41941 tcacttgcaa actgcgagtt cgctggcagc gtcctgctac cgcagagttc cgcttttgcc
    42001 accgtccagc agctcaaaac cagctgggta tacagattcc aggcgagcgg caattcgctg
    42061 aggaacttgt cgctgcaccg cctgcactgc ctgttcaatc gcgaccgcaa aagctcgctg
    42121 attggctgca ccatgacttt ttatcaccgt gccgcgcaat cctaacagac aggcgccgtt
    42181 atactggtcg gggttgaggt gactgaatcg cctcgtcagg ctcttttgta gccaacgctt
    42241 taataacagt agccaccacg accgtttttt cccttcaccc tgagatttca gcagagaaag
    42301 gaacatcctg acaacacctt ccatcgtctt taacgtgaca tttcctgtaa agccgtcaca
    42361 aaccagcaca tctgtcttgc cagttaacaa ctcattggct tcaagatagc cgatataatt
    42421 gatagaaggg attgttttaa gcaccgctga ggcatcccga atactgtcaa gaccctttac
    42481 ttcttcttca ccaatattga gcaacgccac gcgaggattg ggaatttcca ccacctcttc
    42541 agccagaact gagcccataa tggcaaattg caccaacatt gtgctgtcac aatcgacgtt
    42601 ggcccctaaa tcgaggacca ccgttttgcc cttttgctga tgtggtaata ccgtcaccag
    42661 cgccggacgc tcaatcccct ccaggggctt gagtaataat tttgccagcc ccatcagtgc
    42721 cccggtatta ccggcactga cacaggcttg cgctcgacct tctttcacca gctccagggc
    42781 catacgcatt gaactcccac gactggcgcg gatagcttgc gaaggccggg catcactggc
    42841 gataactgac tgcgcaggaa taatctgcag acgcgaacgt tgttcaaagt cagctttagc
    42901 aagtaatggc gtgatggcgt cgggattgcc gactaaaaga agagtgagtt gcgaattaga
    42961 attcagtgcc tgcaatgctg caggcactgt cacggaaggg ccaaaatccc ctcccatgac
    43021 atctaacgcc agggttagac gtgtcaaggt atcgtcgccg cccggtttgg tctttcccca
    43081 gttgcctggg gaaaatcctc actaagcttc atcacgcaga cgcgtgatta cttagcgatg
    43141 accttgcggc cgcggtagta accgtcggca gtgatgtggt gacgcaggtg tttttcacca
    43201 gaagttttgt ctacagacag gctggtgact gcggtcagcg cgtcatggga acgacgcatg
    43261 ccacgtttgg aacgggttgg tttattctgt tgtacggcca tggaccttac tcctcaatta
    43321 cggtttatcc ccgatccaac ggggagcacc aattacttac gctttaagct ggctaatacg
    43381 gcaaatgggt ttggcttttg cacttcttca ggcagttcac caaagaccat gtccgcttcg
    43441 gacacttcac agtgttcaga atcatgcacc ggaactaccg gcaaggcgag gatgatttca
    43501 tcttcaacca ttgcaagcag atcgatttca ccgaattcgt taacctcaat cggttcatac
    43561 gcttccggca gtgcttcagc ctgttcgtct gaacgcacag gactaaaaca atacgttgtg
    43621 tagacctgat gagtaaacgg cttcccgcaa cgctgacact cgagcgttac cgtcaccttc
    43681 gcatcgccgt ttaacactgc gagacgttgg ttatcgatag cgaacgacat ggagcattcc
    43741 acatcactgt ccacactgac tacggattcg gcgacgcgct caacctgatc aggggtatag
    43801 ataccctggt aatcaaggcg tttttgagcc gtacgaaccg gatcgagagt caggggtaat
    43861 tttacctttt gcatagggcg cgcatattaa ctttgtaacg tcatagagtc aaagaaaaag
    43921 gcagcctgtg gttgcctttt gccaataatt cgcacacatt gcgggttact gctttatttt
    43981 cgttcagtgc tgataacgaa catcaacctg caaaacgaag cgcatagttt aaaatggcta
    44041 tcatcaatac gctatatctg atggaaaaaa atatgcctaa acttatttta gcctccacct
    44101 cgccatggcg tcgcgctctt ctggaaaaac tgcaaatctc ttttgaatgt gcagcacctg
    44161 aggtcgacga aaccccacgc agcgacgaat caccgcgaca gttggtgctt cgactggcac
    44221 aagaaaaagc gcaatctctg gcgtcacgtt atccggatca tttaattatt ggttcagacc
    44281 aggtatgtgt tcttgatggc gaaatcaccg gtaaaccgtt aacggaagaa aatgcccgtc
    44341 tgcaattacg caaagccagc ggcaatatcg tcaccttcta tactggactg gcgttgttta
    44401 attcggcgaa tgggcatcta caaacagaag tggagccttt tgacgtccat ttccgtcacc
    44461 taagcgaggc ggagattgat aattacgtgc gtaaggagca tcccctgcac tgcgcgggta
    44521 gctttaagag tgaaggattt ggcattacgc tgtttgagcg tttagaggga cgtgacccta
    44581 acacgctggt tggtttaccg ctaatcgcgc tgtgtcagat gttacgtcgg gaagggaaaa
    44641 acccgctgat gggatagttg tttgcgccgg atgcggttac gtcgcatccg gcaatcggtg
    44701 cagacggcct gatgcggcgt aaacgcctta tcaggccaac cgcccattat caggcttata
    44761 tgcttgtatt agcgcgcgtt acgcagcttt tgcaaacaac gcttcaaacc ttcatccatc
    44821 ggcgcttcga tacgcatcac ctcaccggtc cccggatggg taaacttcaa cgctgcggcg
    44881 tgcaggaaca gacgatttaa tcccgtgcct gcttcagtga gctgtctgtc aaattcacgg
    44941 tcaccgtagc gatcgtcaaa ggcaatcgga tgacccgcat actgtgtatg cacacggatc
    45001 tgatgagtac gtccggttac cgggctacaa cgcaccaggg tggcaaatgc atagcgttct
    45061 tccactttaa agcgtgtttc cgacggtttg ccttcctgac tgacacgcac gatacgttcg
    45121 ccgctttgca gaatattttt caataacggc gcttgaacgc tcttcacatg cgactgccac
    45181 tgaccgcgca ccagcgccag gtaatctttt tgcattcctt tttcacgtaa ttgctcatgc
    45241 agagaacgca acgccgagcg ttttttcgct accagcaaca cacctgaagt gtcccggtcg
    45301 agacgatgaa ccagttcaag gaaccgcgct tccgggcgca acgcccgcaa accttcgata
    45361 acgccgaagc ttaaaccact gccgccatgt accgccgtac cggaaggttt attcagcacc
    45421 aggatatgat catcttcata taagatgacg tccgccagcg ccgccacctt ttgcagatgt
    45481 ggcgaaaccg cctcttcttc ccgctcagca acgcgaaccg gtgggatacg cacctcatca
    45541 cccgcttcga gtttatattc aggcttaata cgttttttgt tcacccgcac ttcaccttta
    45601 cgcaaaatac ggtaaatcat actttttggt acgcctttca attgggtacg caaaaagtta
    45661 tcgatacgtt gccccgcttc gtcagcggtg atagcaacaa tttttacgga tggagtctct
    45721 gttttcatgg tgggcgattc taattagcca acaggattcg cgccactcat ttttctatgc
    45781 ttatatttag ttttgcacct tattacttca ctacgtgatc actttattga tggttattaa
    45841 accaatcacc agcaagaagt gaaaaaactg tgagtaagcg ggtgataaat ggtaaaagtc
    45901 atcttgctat aacaaggctt gcagtggaat aatgaggccg tttccgtgtc catccttgtt
    45961 aaaacaagaa attttacgga ataacccatt ttgcccgacc gatcatccac gcagcaatgg
    46021 cgtaagacgt attgatcttt caggcagtta gcgggctgcg ggttgcagtc cttaccggta
    46081 gatggaaata tttctggaga gtaataccca gtctgtttct ttgataattg cgctgttttt
    46141 ccgcatgaaa aacgggcaac cgacactctg cgcctctttg agctgacgat aaccgtgagg
    46201 ttggcgacgc gactagacac gaggccatcg gttcacaccc ggaaaggcgt tactttgccc
    46261 gcagcttagt cgtcaatgta agaataatga gtaagttacg atgaaaagaa tgttaatcaa
    46321 cgcaactcag caggaagagt tgcgcgttgc ccttgtagat gggcagcgtc tgtatgacct
    46381 ggatatcgaa agtccagggc acgagcagaa aaaggcaaac atctacaaag gtaaaatcac
    46441 ccgcattgaa ccgagtctgg aagctgcttt tgttgattac ggcgctgaac gtcacggttt
    46501 cctcccacta aaagaaattg cccgcgaata tttccctgct aactacagtg ctcatggtcg
    46561 tcccaacatt aaagatgtgt tgcgtgaagg tcaggaagtc attgttcaga tcgataaaga
    46621 agagcgcggc aacaaaggcg cggcattaac cacctttatc agtctggcgg gtagctatct
    46681 ggttctgatg ccaaataacc cgcgcgcagg tggcatttct cgccgtatcg aaggcgatga
    46741 ccgtaccgaa ttaaaagaag cactggcaag ccttgaactg ccggaaggca tggggcttat
    46801 cgtgcgcacc gctggcgtag gcaaatctgc tgaggcgctg caatgggatt taagcttccg
    46861 cctgaaacac tgggaagcca tcaaaaaagc cgctgaaagc cgcccggctc cgttcctgat
    46921 tcatcaggag agcaacgtaa tcgttcgcgc cttccgcgat tatttacgtc aggatatcgg
    46981 cgaaatcctt atcgataacc cgaaagtcct cgaactggca cgtcagcata tcgctgcatt
    47041 aggtcgcccg gatttcagca gcaaaatcaa actgtacacc ggcgagatcc cgctgttcag
    47101 ccactaccag atcgagtcgc agatcgagtc cgccttccag cgtgaagtcc gtctgccatc
    47161 cggtggttcc attgttatcg acagcaccga agcgttaaca gccatcgaca tcaactccgc
    47221 acgcgcgacc cgcggcggcg atatcgaaga aactgcgttt aacaccaacc tcgaagccgc
    47281 cgatgagatt gctcgtcagc tgcgcctgcg tgacctcggc ggcctgattg ttatcgactt
    47341 catcgacatg acgccagtac gccaccagcg tgcggtagaa aaccgtctgc gtgaagcggt
    47401 gcgtcaggac cgtgcgcgta ttcaaatcag ccatatttct cgctttggcc tgctggaaat
    47461 gtcccgccag cgcctgagcc catcactggg tgaatccagc catcacgtct gcccgcgctg
    47521 ctccggtacc ggtaccgtgc gtgacaacga atcgctgtcg ctctctattc tgcgtttgat
    47581 cgaagaagaa gcgctgaaag agaacaccca ggaagttcac gccattgttc ctgtgccaat
    47641 cgcttcttat ctgctgaatg aaaaacgttc tgcggtgaat gcgattgaaa cgcgtcagga
    47701 cggtgttcgc tgcgtgattg tgccaaacga tcagatggaa accccgcact accacgtgtt
    47761 gcgcgtgcgc aaaggggaag aaacgccgac cttaagctac atgctgccga agctgcatga
    47821 agaagcgatg gcgctgccgt ctgaagaaga gttcgctgaa cgtaagcgtc ctgaacaacc
    47881 tgcactggca acctttgcca tgccggatgt accgccagcg ccaaccccag ctgaacctgc
    47941 cgcgactgtc gtagccccag caccgaaagc tgcaacggca acaccagcag ctcctgcaca
    48001 acctgggctg ttaagccgct tcttcggcgc actgaaagcg ctgttcagcg gtggtgaaga
    48061 agccaaaccg accgagcaac caacaccgaa agcagaagcg aaaccggaac gtcaacagga
    48121 tcgtcgcaaa cctcgtcaga gcaaccgccg tgaccgtaat gagcgccgcg acacccgtag
    48181 tgaacgtact gaaggcagcg ataatcgcga agaaaaccgt cgtaatcgtc gccaggcaca
    48241 gcagcaaact gccgagacgc gtgagagccg tcagcaggct gaggtaacgg aaaaagcacg
    48301 taccaccgac gagcagcaag cgccgcgtcg tgaacgtagc cgccgccgta acgatgataa
    48361 acgtcaggcg caacaagaag cgaaggcgct gaatgttgaa gagcaatctg ttcaggaaac
    48421 cgaacaggaa gaacgtgtac gtccggttca gccgcgtcgt aaacagcgtc agctcaatca
    48481 gaaagtgcgt tacgagcaaa gcgtagccga agaagcggta gtcgcaccgg tggttgaaga
    48541 aactgccgct gccgaaccaa ttgttcagga agctccagct ccacgcacag aactggtgaa
    48601 agtcccgctg ccagtcgtag cgcaaactgc accagaacag caagaagaga acaatgctga
    48661 taaccgtgac aacggtggca tgccgcgtcg ttctcgccgc tcgcctcgtc acctgcgcgt
    48721 gagtggtcag cgtcgtcgtc gctatcgtga cgagcgttat ccaacccagt cgccaatgcc
    48781 gttgaccgta gcgtgcacgt ctccggaatt ggcctctggc aaagtctgga tccgctatcc
    48841 aattgtacgt ccgcaagatg tacaagttga agagcagcgc gaacaggaag aagtacaagt
    48901 gcagccgatg gtgactgagg tccctgtcgc cgccgctgtc gaacctgttg ttagcgcgcc
    48961 agttgttgaa gaaatggctg aagtcgtaga agcccccgta ccggttgccg aaccgcaacc
    49021 ggaagtggtt gaaacgacgc atcctgaagt gattgccgcc gcggtaactg aacagccgca
    49081 ggtgattacc gagtctgatg ttgccgtagc ccaggaagtc gcagaacacg cagaaccggt
    49141 ggttgaaccg caggaagaga cggcagatat tgaagaagtt gccgaaactg ctgaggttgt
    49201 ggttgctgaa cctgaagttg ttgctcaacc tgccgcgcca gtcgtcgctg aagtcgcagc
    49261 agaagttgaa acggtggccg cggttgaacc agagatcacc gttgagcata accacgctac
    49321 cgcgccaatg acgcgcgctc cggcaccgga atatgttccg gaggcaccgc gtcacagtga
    49381 ctggcagcgc cctacttttg ccttcgaagg taaaggtgcc gcaggtggtc atacggcaac
    49441 gcatcatgcc tctgccgctc ctgcgcgtcc gcaacctgtt gagtaataat tagctcaaag
    49501 taatcaagcc ctggtaactg ccagggcttt tttatttcat gtttgaatca acatattaaa
    49561 attcatcaaa tggcatcctt gctaaccaac aatgcaaaat aggcaaaaaa atgcccgccc
    49621 cggcgatccc agagcgggca gacaaaacat acccagtttc atgatatgtt ttaaagcgag
    49681 cttatttgtt gagctggaag agcgacaatc cctgcatatc ggtaaatgct ttatacgatg
    49741 cctgcaatgc cgtttgctgc atgatgtaag atgaaatagt tgcattccag tccacatcaa
    49801 ccagatcgct catctgctgc gtttgcccta aagcgcgatc gctacctaag gaatccagcg
    49861 actccagttt gttcagctgc gtgcctaatt ccgcgcgaac ggtaaggaca ttattcagcg
    49921 agtttttcag tccacggttg gttttatcca atgccgcagc ggcgatttct ttatccgctt
    49981 cgctatccgc gaccggcgtt ttcagggctg cgatggcgct atccagcatg gcaaaaagat
    50041 tggtttcaga agcgctaccg tctggttccg ctaccgcgtt actggtaata ctgtcgaaaa
    50101 ttttgtcgcc cgtatgtcct atcaccatcg aacgcgaagc atcgacctgt tgcttaatac
    50161 tttctgttcc gccaacataa tcaccgttag cctcactaaa cggcgcagtc tctgttttat
    50221 aaccggcaaa aatgtagcgc ccattaccgt cagtggtgtt cgccagattc agcaactggt
    50281 cacgaagccc ctgaatatcc gtagccagcg aggcccggtc atcgtcactc aaggtgccat
    50341 tgctggcgta gacaattttt tcctgagcat tctggatagc agtggtgacc tggctaagta
    50401 cactctcttc cagtgacact ttttgagtgg cgaaagtacg tgccagcgtg tactggctgt
    50461 tttgcgcctg tgcctgggag agaacaacag cttgtgatgc agcaatggga tcgtctgaag
    50521 ggttaacgac tcgcttaccc gtcgacatct gctcgccgta cttcatccat tctgcctgag
    50581 aattggtgat gccgcgcatg ttttgctggt acatcatctg ggtactgaaa cgcattttat
    50641 ctccccttag cgaatgttaa tcagcgcatc aaaaatcgcg tttgccgtct gcagaacctg
    50701 cgcattcgcc aggtaatact gctgaaaacg ttgcagattt ccgtactcct catcgagatt
    50761 gacaccggaa atcgactgct gctgattgga aagctgcgtc accacattac cttgcgtggt
    50821 gctactggtt ttcaacgtcg cggttttatt accgatatca ctcactaacg aggcataagc
    50881 gtcgttaaag gatttcgcac cgcccaccgt tttactgttg ctttgcagat ccagcagggc
    50941 ctgaccgttg cggttgtcgc tatcacccgc atcttcttcg ctcgccatcg cgattttcgc
    51001 ttcgtcggtg attaatacat ccatgttgac gatggcgtca cttactggtt tcagcgtgaa
    51061 gctgtcgtta acggcaggcg ttcctgtaaa cgtcaactcc agaccatcaa atgccacttt
    51121 accgttggca tctggtgtcg ccgtaaaagt ggtattgctg gcaaggcggg tgacctgcca
    51181 ctgattatta tcgaacgaga ttttgtaatc tgtcgccagt accacggagg catcagttac
    51241 cgtggcaccg atcgcaacgt cacctttgtt tttcgtgttt tgcagaaccg cgggcttacc
    51301 gatagtaaag aaatcttcac cagcatcgcc gttagcatca aatccggctt tgtgttggct
    51361 gttgaaagcc tcggcaaatg ccagcgccag ttgtccaagc gtattacgcg tctggtccag
    51421 atcctgagaa cggaatgtca gaatgccgcc cagcgacccg gtattcagta atttctccgg
    51481 gatctcaata ttgcctgccg tcccatcaac gtaagcgaca gtcgtacgag aagggtcggc
    51541 gctggaagga actgccgcca gttgccgcgc cgtacttccc tgaaccagtg agtaaccatt
    51601 ggcatcgtga tgttataagt accgccatcc tgaacgctga cttctacgcc aacaatctgg
    51661 tttaattcgc tcaccagttg atcgcgttga tccagcagat tgttaggtga cgcccctgcc
    51721 cccacgcctg tcaggcgcga gatttgatcg ttcaggctgg caatttgttt agcgtaattg
    51781 ttgatctgat caacgctggc acctatcgcg atattgacct gtttgtcctg gtcgcgcaga
    51841 tattgatcgg tggttttaaa ctgattcacc aacccttctg atttcccaat cagcgcctgg
    51901 cgcgccgccg ggtcttccgc gttactcacc agcgtttgca ggctggtgaa gaaatcctgc
    51961 atctgtgttg ccagcgaaga ggtactggtg gagagcatat tgtcgatttt cgacatctgc
    52021 tcatagcggg cggtcaggcc gctactttgc gtctgcgcag cacgtaactg gttggtaata
    52081 aacgtatcat actcacgctg cacaccagaa acgtagacgc cattgccaac ccagccgcca
    52141 gcgcccaacg tgctattggc ctgcgccata atagtggttt ggcgggtata tccggcaacg
    52201 ttatagctgg agatattatt acttgccgta tttaacgccg cctgggccgc gttcagtccg
    52261 ctcatggcgt tattgatcaa gctggacatg gaggttcctt ataagccttc aattacagag
    52321 tattatcggc agcgacccgc cggacttgag ttattcagaa cagattatca atgttcatgc
    52381 tgtaggtttt gctcaccttg tcgcttatcg atttcatctg ctggatcatg ttggtgagtt
    52441 tacgggcata gtgaggatcg gtagcatagc ccgcgtcctg taggacctgc gccccctgtt
    52501 ccgcactcgc ggcggtcgtc acggcggcgt agcgcgggtt acgtgttaac agcccaacgt
    52561 aatccgacaa tgcttccaga tacgagctgt agacccgaaa cttcgctttt actttcttcg
    52621 cttcgccgtt ttcatattca gtcgtggtga tttcagtgac ttgccctttc cagttgccag
    52681 aggctttgac accaaacagg ttatagcttg gctcgccgtt ttcgcggcgg atttgccgtt
    52741 gcccccaacc agattcaagc gcagcctgag cgaggatcaa atgatgtggc acaccgcttt
    52801 gctggcttgc cagttgggcg ggcagcgaga gttgcgcgag gaatgctcta ctgtcacccg
    52861 gcagcgaatc atcgtagtta cgtggtacgg ccttttgcac cagctgcgaa agcgtctggt
    52921 tttgataacg caccacggtt tcgagcggga atttcatcgg tgctgctggc gtggactcct
    52981 ctggcaatgg ttgttctggc gtcatctgtt taaccatcat ctctgcaagc cccagacctt
    53041 tgcccgccgt catctgttgg gcaatttgct ggtcatacat actggtatac agacgagtgt
    53101 gctcgctgct gaacaggcca tcttttggta aagcgtcgcg catgcttttc aacatcatct
    53161 gcacgaacat cccttccacc tgacgggcca ccggacggat atttgccgcc ggatcttcgc
    53221 tcgctttcgc ctttagttcg ttgagtgatt gcgcatccca ggccgcactt gccagtagtt
    53281 tgctgtcgct gatcatcaga tgatttccag ttttgcccgc agacatcccg cactttgcat
    53341 tgattgcaaa atagacatca gatccatcgg cgtagcgccc agcgcattga gcgcacgcac
    53401 cacgttattg aggctggcgc tggaacgtac gctttgcagc gaaccgccgc tctggcgtaa
    53461 atcgatctgc gtttgtggag taacaacagt ctgtccacca ccaaacggtg tatctggctg
    53521 gctgacattg gcctgacgat taactgttac tgagagattt ccctgcgcta ccgcgcagct
    53581 gtcgagggtc acttcgcgat tcatcaccac cgaaccggtg cgcgagttaa tcactacttt
    53641 agcgtcctgc ggggtgacat taacctgcat attctggata tcggcaagga aacggacctg
    53701 ggaactgttg ccacttggta cgcgcacctg aatagtccgc gcatctaacg cggtggcgct
    53761 gccatatcca cgcacgcggt tgatggtgtc agcgatttgc tgcgccatgc tgaaatcttc
    53821 gtcgttaagt tgcaaattaa gggtattccc gacgccaaac tggctgggca attcacgttc
    53881 aataaccgca ccattggtga tccgtccacc gttcagttgg ttaacctgaa cactgctacc
    53941 gcctgcggag gctcctgcgc cgccaaccag aatattgccc tgcgccagcg catacacctg
    54001 actgtcaacg cccttaagcg gtgtcatcaa caacgtacct ccacgcaagc ttttggcatt
    54061 acccatggaa gaaaccacca catcgatggt ttgcccctga cgtccaaacg gaggaagtga
    54121 cgctgtcacc attaccgcag cgacgttttt tagctgcata ttggtgcccg tcggaacggt
    54181 aattcccagc tgtgagagca tgttattaag cgtttgtgtg gtaaacggcg tctgggttgt
    54241 ctggtcacca gtgccatcca gccccactac caagccatag ccaatcagtg agttttgcct
    54301 taccccctga acactggtga gatcgcgaat acgctcagcc tgagccgccg tcgtaaccag
    54361 tagaagaatt aatgcagaga gaaatttaat caccacagcc tcacttacat tggcgacagg
    54421 ttaaggaaga aacgctgcaa ccagcccata ttttgcgctt cgttaatgta gccattgcct
    54481 acgtattcaa tacgcgcatc cgccacctga gtagacggta cggtattgct gccgctgata
    54541 gtgcgtggat taaccacgcc cgagaagcga ataaattcgg taccctgatt aatggcaatc
    54601 tgtttttcac ccaccacatg caggttgccg ttgaccagta cctggtcaac cgtcaccgtc
    54661 aacgtgccgc taaaggtatt gctggcattg gccccgccct ttccgttgaa cgtgttacca
    54721 ccggaggctt cgacatcggc acgagcgtta ccaaacagcc cctgcaaata gcgcggcaca
    54781 gtatcaaagc caaaattagt tttaccgtca cggctggcat tcgcagagga gcttttgctg
    54841 gcgctgacgt tctcctgcaa cacgatggtc agcgtatcgc caatattgcg tggtcgacga
    54901 tcttcaaaca gcggttgata gccatagtta atcggctgag cagactggaa aatagaaccg
    54961 ttggcgacgg gcgtcggacc gggaaccggt tgtgcactgg tcgccccctg caccagcggc
    55021 gtggagggta tccaggcgca gccggttagt gaaagcacca acaagctgga aatggcataa
    55081 gtatgcgcag cgtttttttg catggctatc atcttcaaaa atcagtaaac cggtgaacct
    55141 gccaccggtt atgccttaga gttgcgtcag tttttgcagc atctgatcgg tggtggacac
    55201 cgctttactg ttgatttcgt aagcgcgttg cacctgaatc atattgacca gttcttccgc
    55261 cacgttgacg ttagacgttt caacataccc ttgatacagc agtcccgcgc cgttcaggcc
    55321 cggcgtgctt tcgttcggtg caccggagga ttgcgtttcg gtgtagaggt tttcgccaat
    55381 gctctccagt ccggtatcat tcataaaggt ggtgagattg agctgcccaa cctgaaccgg
    55441 agctgcctgg ccttgttggg ttacgctgac cacgccatca cgaccgatgg tgatacttaa
    55501 cgcattcgcc ggaatggtga tcgctggctg cacctgaaaa ccaccagccg tcaccagctg
    55561 cccgttctga tccacctgga aagagccgtc acggatatag gctgatgagc catctggcaa
    55621 cattacctgg aaaaagccct gccctttaat cgcgacatct ttgctgttat tggtctgcga
    55681 cagattgccc tggctgtgta agcgttcagt ggcgaccggg cgtacccccg tgccgatttg
    55741 taatccggag ggtaaggtgg tttgttcgga agactgtgcc cccggctggc gaatggtttg
    55801 ataaagcaga tcttcaaaca ccgcgcgctg acgcttaaaa ccgttagtac tgacgtttgc
    55861 caggttgttg gcaatgacgt ccatattggt ttgctgggcg tcaaggcccg ttttggcgat
    55921 ccataatgaa ctgatcatat aatgtcctgt agtggattgc tgacaagcag agcagaagca
    55981 cgcgacagtg tgaatttccc ctcaccctaa ccctctcccc aaaggggcga gggaatagtc
    56041 cgcgcgcttt tattgcaatg tcatcaatcg ttcaattaac tcatcgacag cagttggttg
    56101 gcacggcctg cgttatcatc gacgctgctg atcaccttca tctgcatttc aaaacgccgc
    56161 gcgctggcaa tcatgtcgct cattgccgca acggcattga cgttactgcc ttccagaacc
    56221 cccgacatca cacgcaaggt tggatctgcc tgcagtaccg gccctcgcgt ggcctgggtt
    56281 tctgcgctta aacgaaaaat gccgtcgtca ccgcgctgca cttcgctgcc cgtggctttc
    56341 accagtttca gacgccctac tggcgcaact gtatttgccg gatcgcccgg attgagcgcc
    56401 gagattgtgc catcggcagc aatcgtgatt tccgcccctt ccggcacagc aattggcccc
    56461 gcctcgccta tcaccggatg cccctgaatt gtcagttgcc cggtgggatc aacctgaatg
    56521 ctgccattac gcgtataccc ttcgctgccg tcagcggtct gcacggccag ccatcctgct
    56581 gcaacgcaac gtccagcggg cgcgaggtgt aatccatctt gccgggcgtc atgtctgcac
    56641 ccggtgttga cgccgttacc agcgtgcgcg tgggcagtga aagcccttcc accggcactg
    56701 cgcgcaaggc gtttaactgc gcgcgaaaac cgggcgtaga ggcattggcc agattactgg
    56761 cggttaccgc ctgttgattc agcgtctggc tggctgctcc catcgcagta taaattgcgt
    56821 gatccattga gctatcccgt cagcgattag cgtaagttaa cccgcgtgtt gaggatctgg
    56881 tcctgggttt tgatggtctg ggcgttagac ttatagttac gctgggcaac gatcatattg
    56941 accagttctt tactgagatc gacgttggac gcttccagcg caccgttggt cagggtgcca
    57001 aagtttcccg tcccggctgt ccccaacagc gccacgccag aagattgcgt cgcagaccag
    57061 acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa agttcgccag tacaatctgc
    57121 cccagcagtt gggtttgttc gttggaattg ttgccgacaa ccgtaccgtc atcattgatt
    57181 tgataactca ccagatcgcc cggtttgtag ccattctggg tggttgccac aatgttgtta
    57241 gcgccggtat tttgctgcat ggagttgagg aagctcagac taaacgtggc gggttctgca
    57301 ccgttaattg cgccggttgc gatattattc gccatcgcac catccactaa tgtgccatta
    57361 gcattaaatt ccagtgttgt cgctgtcttc gcaatgctgt ttggatcact gctatcctgg
    57421 gtgtagacct gccagttatt atccccggtc ttcacaaaat agacgctcat gtcatgagca
    57481 ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc cgcattgctg
    57541 gcgctgaatg gcgtaacagt aggaagcgga tcactggaat tcaggttgat ctgcatcgac
    57601 gccgtggtgg tagttttcgc tgccatcagg gtattcggga tcgaaatatt ggtcggattc
    57661 gccccttgct gaatagtcgg cggcgtaccg gttaccgggt aacccgtcag ctgtaaacct
    57721 tgcgtattca acaggttacg gttttcatcc agcttaaatt gtccgttacg gctgtagaac
    57781 accgaaccgt tgctgtctac cagacggaaa aaaccgttct ggctgatagc aacgtccaga
    57841 cctcgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc ggcaactttt
    57901 acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt gcctgattta
    57961 aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt ggtggcggca
    58021 gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa tgtgaaggct
    58081 taaattatct gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag atccagcgta
    58141 ttaccgctgt tgccgcggat cacaccctgc accagagcaa actgcagcgg ctgggcaacc
    58201 agttgtgtac caccgttact ggcgctaatc gctacattgt aagaaccgtt cggcgcagtg
    58261 ctgccatcag tcaacgaacc gtcccaggtg aaactgtgaa ctccggcggt cagttcacca
    58321 atatcaatgg tgcgcacaac cgcgccattt ttatcggtga tggtggccgt aactttgtct
    58381 gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg tcacagcccc ttcttcactg
    58441 ccggttcccg caagaacagt tgtgccgggg atcatcacgc cgtgaccgat caggttactg
    58501 gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccgagcgt ggtattgagt
    58561 ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg acgtcagctc gttgttttcc
    58621 attggattgg tcgggtcctg gtttttcagc tgcgccacca gcaaagtcag aaaactgctt
    58681 tgtaaatctg cggcgttgct gcccgtgagc gaactactgc tggtggtact gacgccggta
    58741 tttgtcggat cggtggtggt taccgcaatg gacatagctt tctcctttat tgaccgagcg
    58801 taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg gcctgatagc
    58861 tgcgtgacgc cgacatggtg ttaaccatct ccccgacgac atcaacgttc ggcattttta
    58921 cgtagccctt tgcatctgcc agcggattac ctggttcata aaccagtttg tccggggcct
    58981 gactttctta acatcggcaa cctttacgcc gcctgtcgca gcacctggtg cagcgttaac
    59041 ctggaatacc acctgttttg cccgatatgg ctgtccatca ggaccggtca cgctatcagc
    59101 atttgccaga ttactggccg ccacgttcag gcgctgggac tgggcagtta acgccgaccc
    59161 ggcgatatca aaaatattca gcagtgccat ccgttaattt ccgctctgta aaacgttcat
    59221 catgcctttg atttgcccgc tcaacgcgct aaggctcatc tggtattgca ggctgttatc
    59281 ggcaaactgg gtgcgttcgc gatccatatc gacggtatta ccgtcaagcg aaggctggtc
    59341 cggaatacgg tattgcagtt ctgcggtagg aggcgtcagc gcctgcgccg ggatgtgttg
    59401 cgttgaggtc atcgtcagtg caaccacact ggttgcatcc cgtccacgtt gcatgacttt
    59461 tttaagttca ctggcaaaat cgatatcgcg cgcctgataa ccaggggtat cggcattggc
    59521 gatgtttgct gccagcactt cctgacgctg ggcgcgcaga ttgagcgcct cttgttgaaa
    59581 acgtaaggcg gcgtcgagct tatcgagcat atctcctccg caggtatcaa aattctgcca
    59641 tcagcttaaa tgcctttacg cgcgcgttat cggcggaata aacgcaaaat gggtcgctat
    59701 ttatgccgtt gatggtcatt gcggacaggt acaattcacg ttgtagaaat ggctgggggc
    59761 gaaaatgctg acaataaaac gtagcgtggc gatcatcgcg atactgttca gtccgttaag
    59821 tacggcgagc aatctcacat cgcaattgca caactttttt agcgcccaac tcgcgggggt
    59881 aagtgatgag gttcgtgttt ctattcgtac agcgcccaat ctactaccgc catgcgagca
    59941 gccattgctt tcgatgagca ataattcccg cctgtggggc aatgtgaatg tgttggcacg
    60001 ctgcggtaac gacaaacgat atttacaggt taatgtgcag gccacaggaa attatgtggt
    60061 tgccgcgatg cctattgtgc ggggaggaaa gctggaagct ggcaatgtca aactgaaacg
    60121 tggacgcctg gataccctgc caccgcgtac ggtgctggat atcaatcaac ttgttgatgc
    60181 cgttagcctg cgcgatctat cacccgatca acctatccag ttaacccagt ttcgccaggc
    60241 atggcgggta aaagcgggac aacgcgtcaa tgtgatcgcc agcggtgatg ggtttagcgc
    60301 caacgcagaa ggtcaggcgc tgaacaatgc agccgtcgca cagaatgcgc gggtgcgcat
    60361 ggtatcggga caggtagtca gcggcgttgt tgatgcagat gggaatattc ttataaacct
    60421 gtaagctgtt aaagattacc cgtcccttgc cgataaataa gcaacacatg ataaaagcgc
    60481 cctcaatgag gaataaacca tgagtattga tcgcacttcg cctctgaagc ctgtaagcac
    60541 cgttcaaccg cgcgaaacca ccgacgcgcc ggtaacgaac agccgggcgg caaaaacaac
    60601 cgcctccacc agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga tgcaacccgg
    60661 cagcagtgat atcaatcttg aacgtgtcga agcgttaaaa ctggcgattc gtaacggtga
    60721 actaaaaatg gacaccggca aaattgccga tgcgctgatc aacgaagcgc agcaagactt
    60781 gcagagtaac tgatcgtatg acacgtcttg cagagatcct cgaccagatg tccgctgtgc
    60841 ttaacgatct caaaacggta atggatcaag agcagcaaca tctctctatg gggcagatca
    60901 acggcagcca gttgcaatgg attacagaac aaaaaagctc actgctggcg acgctggatt
    60961 acctcgaaca gttacgcaag aaagaaccca atacagcaaa tagcgttgat attagtcaac
    61021 gctggcagga aattactgtg aaaacgcagc aactacgcca aatgaatcaa cataacggct
    61081 ggttactgga aggacagatt gagcgcaatc aacaggcgct ggaaatgttg aaaccgcatc
    61141 aggaaccgac gctatatggg gcgaacggtc agacctcaac accccatcgc ggcagtaaaa
    61201 agatttcgat ctgaatatta tctccggcct gcactgcagg ccggaatgca ttgttacacc
    61261 gtccggcggg caaattcttt aactttgaag cccagtaccg ccagtgcagc gaagtacgcg
    61321 gcaatccccg ccagcacgac cgccattaaa cgcagtaagc gccagggcat ggtacccaat
    61381 gaccactccg gcatgatatg taacatacct aaaagcacgc cagacatcac cagtaccgcc
    61441 accaccagac gcaacagaaa cgccatccag ccgggttgcg gagtaaagat tttctgctta
    61501 cgcaactgcc agtaaagcag cgaagcattc agacacgccg ccagaccaat agaaagtgac
    61561 agcccggcat gtttcaacgg accaataaac gccaggttca tcaattgcgt taaaatcagc
    61621 gtaacgatgg caattttcac tggcgtttta atgtcctggc gggaataaaa gccaggagcc
    61681 aacactttca ctacaatcag gccaatcaaa cccaccgagt aggcaattaa cgcccgctgg
    61741 gtcatcagcg catcaaacgc ggtaaattta ccgtactgga acagcgaaac ggttaacgga
    61801 cctgaaagaa tgcccaacgc aaccgcactc ggcagtgcca acaggaaaca aagacgcaac
    61861 ccccagtcca tcaaacggtt gtattcatca tgattgccac tggcaaaact tttcgacagc
    61921 gacggcagca aaatggtgcc aagcgccacg cccagcacac cggagggaaa ctccattaag
    61981 cggtcggcgt aatacatcca agacaccgaa ccggaagcaa gaaacgaggc aaaaatggtg
    62041 ttgatgatta aggagatctg gctcacagag acgccaagga tcgccggtcc catctgtttc
    62101 accacgcgca ttgctccagc atcgtggaag ttaatgcgcg gcaggaccag catgccgatc
    62161 ttcttcaggt gcggtagctg atacaccagc tgcaggatgc cgccgaccgt aacagcccac
    62221 gccagcgcca gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa accaatcatg
    62281 ctgatgttaa gcagtgttgg cgcaaacgcc ggaatcgaga agcggttcca cgtattcaga
    62341 atcgctccca ccagcgacgc cagggagatc agcaagatat agggaaaggt aatctttagt
    62401 agctggctgg tcagggcaaa tttgtcagct gtgtcagcga agcctggcgc ggtcaccatg
    62461 atcacccacg gtgcggcgag catgccagcg accgtcacaa ccgccagcgc aagtgtcagc
    62521 aggccagaaa cataagagac aaagacccgc gtggcgtctt caccctgctt acttttatat
    62581 tccgccagaa tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat acggcgtaac
    62641 aagttaggaa gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc gccaaagatt
    62701 ctggcgacaa ttgcgtctcg tgcgaagcca agcacacgcg aaaacatggt catcgagctg
    62761 acggcggcca gcgattttaa taaattcatc ggtgttctaa tccggaccca cgggcaaaac
    62821 gcctgcaagg caggcgctga aagaggcgaa tagtctacct agattatggt gaattgctac
    62881 cgccagatgt tacagggttt attcactcat cgcatcgcgc cagatcttgt caacgatacg
    62941 ttgcgccagc actgcctatt cgccggcggt ttgcggaact gtctgatttt gcacgcattc
    63001 aatgaagtgc cgcgcacagc cgacaaatcc acgttgctca agagtgctct gccaaccagg
    63061 aatcggttta ttcactacac cctgcccgcg ctcctcacgc cattcgcgca tatccgtaat
    63121 gtcgatgagc gcaccgtcag tcacggcctg cacggtttca cgctgacttc cggcacggcg
    63181 atgcatacaa gtggtgatct gcaaaggacc ggccgaaaaa tggtgctcgg caaacagcat
    63241 ttcgccagcg tcgttagtca gtagcgtacc gccatccaga gaggctttgc cgcccgacaa
    63301 ccacagcgcg gtatccacca catgcagata atcatccagc aacgtgaaat aaagatcgtg
    63361 tggcccgacg ctattgctac gatgtttatc cattcttagc gaggctgcgg tggcgagttg
    63421 cgtttttaac tcaccgtaga gtggtgcgaa acgacggtta aaaccgacca tcaacgtcag
    63481 ttttttccgc gccgccagtt ccaccagcct ttcagcatcg cgcagatttt ctgccagcgg
    63541 tttatcgaca cagacatgta cccccgcatt gagtaacgta ctgaccacgt caaagtggct
    63601 ggcggtgctg gagtgcacaa aaaccgcatc acaactggcg gcaaggctgg ataacgaatc
    63661 ggcataagga atgcgccagc tgtcacaaat tggcagggct ttggcgcgcg taggcgacca
    63721 ggctccttgt aacgtccagt cagacgctgc cgccagcacc ggtaaccacg ctttttgcgc
    63781 aatgccgcct aatcccacta cgccgatacg taattttttc actttaatct cccagatggg
    63841 ccagcaacga atcaagacgt tgtttcagtt ctgccacttc gatttccagg gcttcgacgc
    63901 gggtctataa atcaccgtca accatgttcg acatagccgt taccgccggc tgatcttcaa
    63961 cctcaccact gaaaagatgc atgtagcggc tttcgcgttt acccggttcg cgggccagac
    64021 gcaccacaaa aggaccatct tcgcgattcg ccagttgttc cagcgtcgac tccacttccg
    64081 ccatatcgct gaattcatac attcgcgcgg cgcggctgcg cagttcaccc ggcgtctggg
    64141 caccacgcaa taacaacgtg gtgattaacg ccacttctgc ggcgctcagt ttcagatcgc
    64201 caaattctga attacaaaaa cgttgctcat atttggtgac ccgattacca aaaccgctca
    64261 ctgtgcgtag ataatgacgt ttgaccagat tatccagctg ttcctgcact tcggattcac
    64321 tcagattcat gaccggttta cggttcgttt tctgattaca ggccgtgact acaccattga
    64381 ctgagagcgg gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa ccaattacgc
    64441 gagcttccaa ggcagtaagt tgatatttca tgagattctc cttagcggcc gggcgtccag
    64501 tctggggtag ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat caacagatag
    64561 tctttcgcat agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc gctgcgttta
    64621 ttgtgcggca tataattagc cataatgcga tgaatatgtt gggtgcgctg catataacga
    64681 atggctgcgg tcagggcttc aaacatgagt cctttgccct gccatttttg cccaatcgaa
    64741 taaccgagat agcaggcatg aaaagagccg cgaacaacat tggaaaaatt ggcaacgcca
    64801 ataatctctt tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc ttgtttatga
    64861 aattcgttaa tcatccctag cctggcctgc cagcctgatg gataacagtg gctttcgtcg
    64921 cgcactggct cccagggctt gaggaaatgg cgattctctg cgtaataatc cgccagacgc
    64981 caggcatcac gatcatgcac cagacgcacg accagtcggt ctgtggttaa gcgcactttt
    65041 ggcacgttac tgcgatagcc aaacattcta tacctactcc ttcccgtaac gtctttaata
    65101 cgctgtaagt attactatac ctgcgcaacc agaccctgtg aaaagtccaa aatgccattt
    65161 tctgcattat tcacatattt gataaaaact ctctatcaaa gcgacgtttt gataaaaaaa
    65221 tattgtcccc gaagtgatgg gaaaagtctt agcggcaatg cagaatagaa agtgctcatc
    65281 ttttatttcc cgggagggga aatgtcccgc gtatcgcagg cgaggaacct gggtaaatat
    65341 ttcctgctca tcgataatat gctggtcgtg ctggggttct ttgttgtctt cccgctgatc
    65401 tctatccgct tcgttgatca aatgggctgg gccgccgtca tggtcggtat tgctctcggt
    65461 ctacgccagt ttattcagca aggtctgggt attttcggcg gcgcaattgc cgaccgcttt
    65521 ggtgccaaac cgatgattgt taccggtatg ctgatgcgcg ccgccggatt cgccacaatg
    65581 ggtatcgccc acgaaccgtg gctattgtgg ttttcatgcc tgctctcggg actcggcggc
    65641 acgttgtttg atccgccgcg ttcggcgctg gtggtgaaac taatccgtcc acaacaacgt
    65701 ggtcgttttt tctcgctgtt gatgatgcag gacagtgcca gtgcggtcat tggcgcattg
    65761 ttggggagct ggctgttgca atacgacttt cgcctggtct gcgccacagg ggccgttttg
    65821 tttgtgctat gtgcggcgtt caatgcgtgg ttgttaccgg catggaaact ctccaccgta
    65881 cgcacgcccg ttcgcgaagg catgacccgc gtgatgcgtg acaagcgttt tgtcacctat
    65941 gtcctgacgc tggcgggtta ctacatgctg gctgtacaag tgatgctgcc aattatggtc
    66001 aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga agcgtgtctg
    66061 tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg tctggaacac
    66121 cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg catggtcagc
    66181 ggcctacaac aacttttcac cctgatttgt ctgttttata tcgggtcgat cattgccgag
    66241 cctgcgcgtg aaaccttaag tgcttcgctg gcggacgcaa gagctcgcgg cagctatatg
    66301 gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg tggtggctgg
    66361 ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat gctgggaatt
    66421 attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg cgccgcgcgt
    66481 cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca tactgaaagt
    66541 gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca ctgcccttac
    66601 tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt acatgttctg
    66661 ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt tcccattctg
    66721 caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta tgtcgataaa
    66781 ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga gtggctgcgc
    66841 aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc ggcatttgat
    66901 gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc cagcgcgggt
    66961 aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag cgatgatttg
    67021 cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggcgaact ttcggaagat
    67081 gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat caactggcca
    67141 agccgcgttg ctaattaccg cgataatatg gcgaaacaga cacaaatcaa tttgttatca
    67201 tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt gccgccgttt
    67261 tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt gtcaggatat
    67321 tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg ctgcattgat
    67381 cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca ccgaacaaga
    67441 aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt tacccggcgt
    67501 ggctgacgcc catattgttc tgacaaactt gaccagccaa attggtcgcg aagagccgaa
    67561 taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg gtagtcagaa
    67621 agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa aaggtgcgat
    67681 cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa tgcaaacggt
    67741 gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta accagcaacc
    67801 tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa aactggcgaa
    67861 aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat gacgagggcg
    67921 cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct ttgtgtccgg
    67981 caaaaacatc ccttcagccg gagcatagag attaatgact gcaccatccc aggtattaaa
    68041 gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt taaaaactgt
    68101 cgtgccgtat accagcgaaa tttatggacg ggctatcgta atgcccaatc tggctccgcc
    68161 cgtgaccaca gttgaggctg ccgtggcgta tcgccaacgt attcttgacg ccgtacctgc
    68221 cgggcacgat ttcaccccat tgatgacctg ttatttaaca gattcgctgg atcctaatga
    68281 gctggagcgc ggatttaatg aagccgtgtt caccgctgca aaactttacc cggcaaacgc
    68341 aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg tacttgagcg
    68401 catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg cagatatcga
    68461 catttttgat cgtgaagcgc gctttataga aagcgtgatg gaacctctgc gccagcgcct
    68521 gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg actatgtccg
    68581 tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt ttaaccgcaa
    68641 ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc tcaaacgcaa
    68701 tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag tattcctcgg
    68761 tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct gcgcgggctg
    68821 cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga tgaatgctct
    68881 gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatggct tgccggtcaa
    68941 cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca tcgcactgac
    69001 tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg ttaaacaata
    69061 aaaatcgtag cttcctgttg tcattaggtt attttacctg tataaataac cagtatgttc
    69121 aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca ttgccagctg
    69181 gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg tttcctgata
    69241 atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt attggcgcaa
    69301 caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa agcgccgatg
    69361 actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccggatcg ccccggcttt
    69421 tttattttac ttacgaattt tgagtacaaa atttctacac ttcctcaaaa taattgcaca
    69481 atatgctaaa aaaatatgaa tgacatgctg ttttattgtt catataaatc ttaaaagcag
    69541 aaatttatct gttcccccct ttaaaaattc ggtgaatacc cttacttatt ggtatactga
    69601 agttgcttca gataaaaacg cattgaacct cgaataacgt tgtctagtaa cacgaattag
    69661 ggggccatga tggaaaaaaa taatgaagtc attcagactc atccgctcgt agggtgggac
    69721 atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt tgcactacca gaccccaaat
    69781 aagtccgagc aggaagggac tgaagttggt cagacgctct ggttaaccac tgatgttgcc
    69841 agacaattta tttcgatatt agaagcagga atcgccaaaa ttgaatccgg tgatttcccg
    69901 gtaaacgagt atcggcgtca ttaacatgac atgaccatcc acccaaaagg cacctcaccg
    69961 gtgcctttac catttctggg ttgtatatct gcattcagtt aattactctg cttaaaccat
    70021 gagagcagag agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg ttccgtaggc
    70081 gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac cgacgcccat
    70141 atgccaccgc atcaacacgg cagccaccac ggcgatacgc gattaattcg ccatgcttat
    70201 ggtgaaggcg aaaagtatgt cccgttggtc ctccgcgcgc aaacgctgtg ggatgaactt
    70261 tcccgccaca acgaagatga tcccattttt gtacgctctg gtgtcattaa cctcggcccg
    70321 gctgactccg catttctcgc caacgtcgcc cacagcgccg aacagtggca actcaacgtt
    70381 gaaaagctcg atgcgcaagg gattatggcc cgctggccag aaatacgcgt cccggacaac
    70441 tacatcggct tatttgagac tgattccggt tttttgcgca gcgaactggc gattaaaacc
    70501 tggatccaac tggcgaagga agcgggctgt gcgcaactgt tcaactgccc ggtcaccgca
    70561 attcgtcatg acgatgatgg cgtaactatt gaaacggttg acggtgagta tcaggcgaaa
    70621 aaagcgattg tctgcgcggg aacatgggta aaagacctgc tcccggagct gcctgtccag
    70681 cccgtacgta aagtatttgc ctggtatcag gccgatggcc gctatagcgt gaagaataaa
    70741 ttcccggcgt ttaccggtga actgcccaat ggcgatcaat attatggttt tccggcagaa
    70801 aacgacgcgt tgaagattgg caaacataac ggaggccagg ttatccattc agcggatgaa
    70861 cgtgttccgt ttgcggaagt ggccagtgat gggtcagaag ccttcccgtt cttgcgcaat
    70921 gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct gcacctatga taattcgcct
    70981 gacgaagatt ttattatcga taccctaccc ggccacgata atacactgct cattaccggc
    71041 ctgagtgggc acggttttaa atttgcgtca gttttagggg aaatagctgc cgattttgcg
    71101 caagaccaaa aaagcgattt tgatttgacg ccattcaggc tttcccgctt ccaataatca
    71161 tcattacggc ctccagaatg aggccgtttt tacaggagta tttaatgcgt cggctgttgc
    71221 actatctcat caataatatt cgcgagcatt tgatgcttta tctttttctc tggggattgt
    71281 tggcaattat ggatctgatc tatgtgtttt atttttagtt ttccgcactg acataaactg
    71341 acaaatattt aatctccctt tcatttagcg tgaaaaattg aatgatatta ttcctgcttc
    71401 tacagcactc accagacatc tctgatgctg aacccactaa acttatgaag attctttcat
    71461 tattaattca aaattattga aataaaaata aatatcattc aatttgttat ttttgtgttg
    71521 caattcattc tccgacacgc cattttcttt ctctcgcaaa ttcgtattgg atgcacttat
    71581 gtcattcaca aatacccctg aacgctatgg tgttatatct gcggccttcc attggctaag
    71641 cgcaattatt gtctacggca tgtttgcttt aggtctgtgg atggtcacgc tcagttatta
    71701 cgatggctgg tatcacaaag cacccgaact gcataaaagc atcggtattt tgttaatgat
    71761 ggggctggtt attcgcgttc tgtggcgtgt catatctcca ccgccgggtc cgctgccgag
    71821 ttattcgccc atgactcgtc ttgccgcaaa agctggacat ctcgcattgt acctattgct
    71881 gttcgccatc ggcatcagcg gctatctgat ctcaactgcc gatggtaaac cgatcagcgt
    71941 ttttggctgg tttgacgtcc ccgcgaccct tgccgacgct ggcgcacagg cagactttgc
    72001 cggtgccctg catttttggc tcgcgtggag cgtcgtagta ctgtctgtta tgcacggatt
    72061 tatggccctg aagcatcatt tcatcgataa agacgacact ctgaagcgca tgctgggaaa
    72121 gtcgtcatct gactatggag tataaaaaat gaaaaaaagc ctgcttggtt taaccttcgc
    72181 gtccctgatg ttctctgccg gttcagcggt tgccgccgat tacaaaattg acaaagaagg
    72241 tcagcacgcc tttgttaact tccgcataca gcatcttggc tatagctggt tatatggcac
    72301 ctttaaagat ttcgacggta cttttacctt tgatgaaaaa aatccggctg ccgataaagt
    72361 gaatgtgaca attaacacca ccagcgtcga tactaaccac gccgaacgcg ataaacatct
    72421 tcgcagtgcg gattttctca ataccacaaa atatccacag gcaacattca cctccaccag
    72481 cgtgaagaaa gacggtgacg aactggatat taccggcgat ctgacgctga atggcgtaac
    72541 caaacccgtc acgctggaag cgaaattaat tggtcagggc gacgacccat ggggcggtaa
    72601 acgtgcaggc tttgaggccg aaggcaaaat taagctcaaa gacttcaata tcaagacaga
    72661 tttaggtcca gcttctcagg aagtggatct gattatttca gtggaaggcg tacagcagaa
    72721 gtaagagtga aatgttgccg gatggcacgc atccggcaat gatatttatt ctgttggatc
    72781 aggaataccc agtgttgtat tcagacgtcc acgagactta ttaaagatct tattgccatt
    72841 ttcacgtcct gcccgacggc gtcgctgttc ttctggcggt aacgcgcttt cttcgcagca
    72901 aatctcacta caacaacctt tgtatttttc cgcgcatact ggacactgaa taaacagcag
    72961 gtggcagcca tcatttttac agttggtatg gctgtcgcac ggcgcgccgc actggtggca
    73021 atgcgcgata atctcatccg atatacgttc gcccatccgc tcgtcaaaaa caaaattttt
    73081 gccaataaaa cgcaccggca agccctgctc gcgcgcctta cgggcgtatt caataattcc
    73141 gccctcgata tgccagactt tgttgaatcc gttatgtttc atccaggcac tggccttttc
    73201 acaacgaatg ccgccggtgc agtacatgac gattttttta tctttatgtg cctgcatcat
    73261 ctcgactgct tttggcagct gctcacggaa ggtatctgcc ggaatttcga gcgcgttttc
    73321 aaagtgcccc acttcatact catagtggtt acgcatgtcg ataaatagtg catcgggatc
    73381 gtcaagcatg gcgttcactt ccgccgcttg cagatactca ccaacattgc tggcatcaaa
    73441 gtgaggatcg tcaataccgt cggcaacaat gcgatcgcgt accttcatgc gcagtaccca
    73501 gaaggatttc ccgtcatcat ccaacgcgat attcaggcgt aaaccctcta aagccgggtc
    73561 gaaggcatag agctgcgcgc gaaatgtttc aacattgctc gcaggtacgc tgatttgcgc
    73621 gttaatgccc tcatgcgcca gatacactcg cccaaaaaca ttcagcgcgg taaacagctg
    73681 atataaagcg tcacgggtcg ccttaggatc ggcgatgtgg aaatacttgt aaaacgaaat
    73741 ggtggttcgc ggttcgctct cagccaacat tttggctttt aacgcgtcgt tggaaatgcg
    73801 gttgtgtaac actggcatgg tgtacggttc ctgcgagatg ggaaagtaaa aatccgcggc
    73861 atgatatagc aattatcgat aattaacatc cacacatttt acgctacatt tgcgcattaa
    73921 aaattatttg ttatttacaa gcgcggcaat ttcgcccagt cttcagccac aaattttggt
    73981 tgcgggcgaa aaaatgcgac aatacataca attgcccgta taggttgaaa aacaggattg
    74041 atatgacgaa tctacccaag ttctccaccg cactgcttca tccgcgttat tggttaacct
    74101 ggttgggtat tggcgtactt tggttggtcg tgcaattgcc ctacccgatt atctaccgcc
    74161 tcggttgtgg attaggaaaa ctggcgttac gttttatgaa acgacgcgca aaaattgtgc
    74221 atcgcaacct ggaactgtgc ttcccggaaa tgagcgaaca agaacgccgt aaaatggtgg
    74281 tgaagaattt cgaatccgtt ggcatgggcc tgatggaaac cggtatggcg tggttctggc
    74341 cggaccgccg aatcgcccgc tggacggaag tgatcggcat ggaacacatt cgtgacgtgc
    74401 aggcgcaaaa acgcggcatc ctgttagttg gcatccattt tctgacactg gagctgggcg
    74461 cgcggcagtt tggtatgcag gaaccgggga ttggcgttta ccgcccgaac gataatccac
    74521 tgattgactg gctacaaacc tggggccgtt tgcgctcaaa taaatcgatg ctcgaccgca
    74581 aagatttaaa aggcatgatt aaagccctga aaaaaggcga agtggtctgg tacgcaccag
    74641 atcatgatta cggcccgcgc tcaagcgttt tcgtcccgtt atttgccgtt gagcaggctg
    74701 cgaccacgac cggaacctgg atgctggcac ggatgtccgg cgcatgtctg gtgcccttcg
    74761 ttccacgccg taagccagat ggcaaagggt atcaattgat tatgctgcca ccagagtgtt
    74821 ctccgccact ggatgatgcc gaaactaccg ccgcgtggat gaacaaagtg gtcgaaaaat
    74881 gcatcatgat ggcaccagag cagtatatgt ggttacaccg tcgctttaaa acacgcccgg
    74941 aaggcgttcc ttcacgctat taaatctccc atgccggatg cttcagaatg gcatccggca
    75001 ttaccacagc aaatccccct gatttagcga taaaagctct ctggattgcg ccccctggaa
    75061 gtcgggcgca taattagtgt gtttatcttt tcttcttatg ttcaccgcgc ctggcgcacc
    75121 aacagcggat tgctatgtca ccctgtgaaa atgacacccc tataaactgg aaacgaaacc
    75181 tgatcgtcgc ctggctaggc tgttttctta ccggggccgc cttcagtctg gtaatgccct
    75241 tcttacccct ctacgttgag cagcttggtg ttaccggcca ctccgccctg aatatgtggt
    75301 ccggtattgt cttcagtata atgggttcct aaacacatca ttttaggtat tgaatatgag
    75361 acgggcaatc agctacatca gattttcaag tgaacgacaa ctgaaagggg actctgtacg
    75421 ccgacaatct aagctggtta ctgactggct tgataagaac cctgagtttt acttggattc
    75481 atccttgagc tttaaagatt tggggaaaag tgcttttagt ggtaagcacc tgaaaggtgg
    75541 tctaggtgac tttttaaccg ctatcgagaa agggcttgtt aaagctggtg atacgctact
    75601 aatcgagagt ttagaccgtc tgtcacgtca ggatattgat attgcttctg agctattacg
    75661 ccgtatctta agggctggtg tagatgtagt cacactgagc gatggagaac attacacacg
    75721 agaaagttta aaagacccgt tagccctgat taaaagcatt ctgataatgc agcgggcgca
    75781 tgaggaaagt ttgagaaagt ctgagcgtgt ccaagctgca tggaaccgta aaaaagaact
    75841 catctccgaa ggtatcaagg tat
//
LOCUS       DAEEGA010000004        75618 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEEGA010000004 DAEEGA010000000
VERSION     DAEEGA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75618
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.004"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(437..643)
                     /locus_tag="KLH46_000246"
     CDS             complement(437..643)
                     /locus_tag="KLH46_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBE0377084.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            956..1045
                     /gene="kdpF"
                     /locus_tag="KLH46_000247"
     CDS             956..1045
                     /gene="kdpF"
                     /locus_tag="KLH46_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBE0377085.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1045..2718
                     /gene="kdpA"
                     /locus_tag="KLH46_000248"
     CDS             1045..2718
                     /gene="kdpA"
                     /locus_tag="KLH46_000248"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBE0377086.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2741..4788
                     /gene="kdpB"
                     /locus_tag="KLH46_000249"
                     /pseudo
     CDS             2741..4788
                     /gene="kdpB"
                     /locus_tag="KLH46_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4797..5369
                     /gene="kdpC"
                     /locus_tag="KLH46_000250"
     CDS             4797..5369
                     /gene="kdpC"
                     /locus_tag="KLH46_000250"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBE0377087.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5362..8042
                     /gene="kdpD"
                     /locus_tag="KLH46_000251"
                     /pseudo
     CDS             5362..8042
                     /gene="kdpD"
                     /locus_tag="KLH46_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8039..8716
                     /gene="kdpE"
                     /locus_tag="KLH46_000252"
     CDS             8039..8716
                     /gene="kdpE"
                     /locus_tag="KLH46_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBE0377088.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8991..9095
                     /locus_tag="KLH46_000253"
     CDS             8991..9095
                     /locus_tag="KLH46_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBE0377089.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9406..11603
                     /gene="speF"
                     /locus_tag="KLH46_000254"
                     /pseudo
     CDS             9406..11603
                     /gene="speF"
                     /locus_tag="KLH46_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11600..12919
                     /gene="potE"
                     /locus_tag="KLH46_000255"
     CDS             11600..12919
                     /gene="potE"
                     /locus_tag="KLH46_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBE0377090.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12975..14615)
                     /gene="pgm"
                     /locus_tag="KLH46_000256"
     CDS             complement(12975..14615)
                     /gene="pgm"
                     /locus_tag="KLH46_000256"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBE0377091.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14641..15186)
                     /gene="seqA"
                     /locus_tag="KLH46_000257"
     CDS             complement(14641..15186)
                     /gene="seqA"
                     /locus_tag="KLH46_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBE0377092.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15371..16135
                     /gene="ybfF"
                     /locus_tag="KLH46_000258"
     CDS             15371..16135
                     /gene="ybfF"
                     /locus_tag="KLH46_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBE0377093.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16275..16568
                     /gene="ybfE"
                     /locus_tag="KLH46_000259"
     CDS             16275..16568
                     /gene="ybfE"
                     /locus_tag="KLH46_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBE0377094.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16708..17238
                     /gene="fldA"
                     /locus_tag="KLH46_000260"
     CDS             16708..17238
                     /gene="fldA"
                     /locus_tag="KLH46_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBE0377095.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17448..17534
                     /gene="uof"
                     /locus_tag="KLH46_000261"
     CDS             17448..17534
                     /gene="uof"
                     /locus_tag="KLH46_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBE0377096.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17527..17973
                     /gene="fur"
                     /locus_tag="KLH46_000262"
     CDS             17527..17973
                     /gene="fur"
                     /locus_tag="KLH46_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBE0377097.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18057..18383)
                     /locus_tag="KLH46_000263"
     CDS             complement(18057..18383)
                     /locus_tag="KLH46_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377098.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18420..19826)
                     /gene="chiP"
                     /locus_tag="KLH46_000264"
     CDS             complement(18420..19826)
                     /gene="chiP"
                     /locus_tag="KLH46_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBE0377099.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20405..22069)
                     /gene="glnS"
                     /locus_tag="KLH46_000265"
     CDS             complement(20405..22069)
                     /gene="glnS"
                     /locus_tag="KLH46_000265"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBE0377100.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22272..24218)
                     /gene="nagE"
                     /locus_tag="KLH46_000266"
     CDS             complement(22272..24218)
                     /gene="nagE"
                     /locus_tag="KLH46_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBE0377101.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24551..25351
                     /gene="nagB"
                     /locus_tag="KLH46_000267"
     CDS             24551..25351
                     /gene="nagB"
                     /locus_tag="KLH46_000267"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBE0377102.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25411..26559
                     /gene="nagA"
                     /locus_tag="KLH46_000268"
     CDS             25411..26559
                     /gene="nagA"
                     /locus_tag="KLH46_000268"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBE0377103.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26568..27788
                     /gene="nagC"
                     /locus_tag="KLH46_000269"
                     /gene_synonym="nagR"
     CDS             26568..27788
                     /gene="nagC"
                     /locus_tag="KLH46_000269"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBE0377104.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27836..28588
                     /gene="nagD"
                     /locus_tag="KLH46_000270"
     CDS             27836..28588
                     /gene="nagD"
                     /locus_tag="KLH46_000270"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBE0377105.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28985..30649
                     /gene="asnB"
                     /locus_tag="KLH46_000271"
     CDS             28985..30649
                     /gene="asnB"
                     /locus_tag="KLH46_000271"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBE0377106.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31028..31104
                     /locus_tag="KLH46_000272"
     tRNA            31028..31104
                     /locus_tag="KLH46_000272"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31062..31064,aa:Met,seq:cat)
     gene            31113..31197
                     /locus_tag="KLH46_000273"
     tRNA            31113..31197
                     /locus_tag="KLH46_000273"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31147..31149,aa:Leu,seq:tag)
     gene            31221..31295
                     /locus_tag="KLH46_000274"
     tRNA            31221..31295
                     /locus_tag="KLH46_000274"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31253..31255,aa:Gln,seq:ttg)
     gene            31330..31404
                     /locus_tag="KLH46_000275"
     tRNA            31330..31404
                     /locus_tag="KLH46_000275"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31362..31364,aa:Gln,seq:ttg)
     gene            31420..31496
                     /locus_tag="KLH46_000276"
     tRNA            31420..31496
                     /locus_tag="KLH46_000276"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31454..31456,aa:Met,seq:cat)
     gene            31545..31619
                     /locus_tag="KLH46_000277"
     tRNA            31545..31619
                     /locus_tag="KLH46_000277"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31577..31579,aa:Gln,seq:ctg)
     gene            31657..31731
                     /locus_tag="KLH46_000278"
     tRNA            31657..31731
                     /locus_tag="KLH46_000278"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31689..31691,aa:Gln,seq:ctg)
     gene            complement(31885..33060)
                     /gene="ubiF"
                     /locus_tag="KLH46_000279"
     CDS             complement(31885..33060)
                     /gene="ubiF"
                     /locus_tag="KLH46_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBE0377107.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33206..34630
                     /gene="miaB"
                     /locus_tag="KLH46_000280"
     CDS             33206..34630
                     /gene="miaB"
                     /locus_tag="KLH46_000280"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBE0377108.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34783..35823
                     /locus_tag="KLH46_000281"
     CDS             34783..35823
                     /locus_tag="KLH46_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBE0377109.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35820..36287
                     /gene="ybeY"
                     /locus_tag="KLH46_000282"
     CDS             35820..36287
                     /gene="ybeY"
                     /locus_tag="KLH46_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBE0377110.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36377..37255
                     /gene="corC"
                     /locus_tag="KLH46_000283"
     CDS             36377..37255
                     /gene="corC"
                     /locus_tag="KLH46_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBE0377111.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37280..38818
                     /gene="lnt"
                     /locus_tag="KLH46_000284"
     CDS             37280..38818
                     /gene="lnt"
                     /locus_tag="KLH46_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBE0377112.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39215..40123
                     /gene="gltI"
                     /locus_tag="KLH46_000285"
     CDS             39215..40123
                     /gene="gltI"
                     /locus_tag="KLH46_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBE0377113.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40293..41033
                     /gene="gltJ"
                     /locus_tag="KLH46_000286"
     CDS             40293..41033
                     /gene="gltJ"
                     /locus_tag="KLH46_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBE0377114.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41033..41707
                     /gene="gltK"
                     /locus_tag="KLH46_000287"
     CDS             41033..41707
                     /gene="gltK"
                     /locus_tag="KLH46_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBE0377115.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41707..42432
                     /gene="gltL"
                     /locus_tag="KLH46_000288"
     CDS             41707..42432
                     /gene="gltL"
                     /locus_tag="KLH46_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBE0377116.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42550..43485
                     /gene="rihA"
                     /locus_tag="KLH46_000289"
     CDS             42550..43485
                     /gene="rihA"
                     /locus_tag="KLH46_000289"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBE0377117.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43569..45230
                     /gene="hscC"
                     /locus_tag="KLH46_000290"
                     /pseudo
     CDS             43569..45230
                     /gene="hscC"
                     /locus_tag="KLH46_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45290..46741)
                     /gene="djlC"
                     /locus_tag="KLH46_000291"
     CDS             complement(45290..46741)
                     /gene="djlC"
                     /locus_tag="KLH46_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBE0377118.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46738..47445)
                     /locus_tag="KLH46_000292"
     CDS             complement(46738..47445)
                     /locus_tag="KLH46_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBE0377119.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47547..48101
                     /locus_tag="KLH46_000293"
     CDS             47547..48101
                     /locus_tag="KLH46_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBE0377120.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLRWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48111..49538)
                     /locus_tag="KLH46_000294"
     CDS             complement(48111..49538)
                     /locus_tag="KLH46_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBE0377121.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHEGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49535..50242)
                     /locus_tag="KLH46_000295"
     CDS             complement(49535..50242)
                     /locus_tag="KLH46_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBE0377122.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50406..51383
                     /locus_tag="KLH46_000296"
     CDS             50406..51383
                     /locus_tag="KLH46_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBE0377123.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51453..51935)
                     /locus_tag="KLH46_000297"
     CDS             complement(51453..51935)
                     /locus_tag="KLH46_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBE0377124.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52170..54752
                     /gene="leuS"
                     /locus_tag="KLH46_000298"
     CDS             52170..54752
                     /gene="leuS"
                     /locus_tag="KLH46_000298"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBE0377125.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54767..55348
                     /gene="lptE"
                     /locus_tag="KLH46_000299"
     CDS             54767..55348
                     /gene="lptE"
                     /locus_tag="KLH46_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBE0377126.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55348..56379
                     /gene="holA"
                     /locus_tag="KLH46_000300"
     CDS             55348..56379
                     /gene="holA"
                     /locus_tag="KLH46_000300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBE0377127.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56381..57022
                     /gene="nadD"
                     /locus_tag="KLH46_000301"
     CDS             56381..57022
                     /gene="nadD"
                     /locus_tag="KLH46_000301"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBE0377128.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57046..57657
                     /locus_tag="KLH46_000302"
     CDS             57046..57657
                     /locus_tag="KLH46_000302"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBE0377129.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57917..58234
                     /gene="rsfS"
                     /locus_tag="KLH46_000303"
                     /gene_synonym="rsfA"
     CDS             57917..58234
                     /gene="rsfS"
                     /locus_tag="KLH46_000303"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBE0377130.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58238..58705
                     /gene="rlmH"
                     /locus_tag="KLH46_000304"
     CDS             58238..58705
                     /gene="rlmH"
                     /locus_tag="KLH46_000304"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBE0377131.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58736..60637
                     /gene="mrdA"
                     /locus_tag="KLH46_000305"
     CDS             58736..60637
                     /gene="mrdA"
                     /locus_tag="KLH46_000305"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBE0377132.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60640..61752
                     /gene="mrdB"
                     /locus_tag="KLH46_000306"
                     /gene_synonym="rodA"
     CDS             60640..61752
                     /gene="mrdB"
                     /locus_tag="KLH46_000306"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBE0377133.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61763..62851
                     /gene="rlpA"
                     /locus_tag="KLH46_000307"
     CDS             61763..62851
                     /gene="rlpA"
                     /locus_tag="KLH46_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBE0377134.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            62990..64201
                     /gene="dacA"
                     /locus_tag="KLH46_000308"
     CDS             62990..64201
                     /gene="dacA"
                     /locus_tag="KLH46_000308"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBE0377135.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64312..64575
                     /locus_tag="KLH46_000309"
     CDS             64312..64575
                     /locus_tag="KLH46_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBE0377136.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64676..65317
                     /gene="lipB"
                     /locus_tag="KLH46_000310"
     CDS             64676..65317
                     /gene="lipB"
                     /locus_tag="KLH46_000310"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBE0377137.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65576..66529
                     /locus_tag="KLH46_000311"
     CDS             65576..66529
                     /locus_tag="KLH46_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBE0377138.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66738..67703
                     /gene="lipA"
                     /locus_tag="KLH46_000312"
     CDS             66738..67703
                     /gene="lipA"
                     /locus_tag="KLH46_000312"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBE0377139.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67804..68007)
                     /gene="tatE"
                     /locus_tag="KLH46_000313"
     CDS             complement(67804..68007)
                     /gene="tatE"
                     /locus_tag="KLH46_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBE0377140.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68136..68924)
                     /locus_tag="KLH46_000314"
     CDS             complement(68136..68924)
                     /locus_tag="KLH46_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBE0377141.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69017..69400
                     /gene="crcB"
                     /locus_tag="KLH46_000315"
     CDS             69017..69400
                     /gene="crcB"
                     /locus_tag="KLH46_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBE0377142.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69454..69663)
                     /gene="cspE"
                     /locus_tag="KLH46_000316"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69454..69663)
                     /gene="cspE"
                     /locus_tag="KLH46_000316"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBE0377143.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69838..70398)
                     /gene="pagP"
                     /locus_tag="KLH46_000317"
     CDS             complement(69838..70398)
                     /gene="pagP"
                     /locus_tag="KLH46_000317"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBE0377144.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            70986..72371
                     /gene="dcuC"
                     /locus_tag="KLH46_000318"
     CDS             70986..72371
                     /gene="dcuC"
                     /locus_tag="KLH46_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBE0377145.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTDK"
     gene            complement(72412..72753)
                     /locus_tag="KLH46_000319"
                     /pseudo
     CDS             complement(72412..72753)
                     /locus_tag="KLH46_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73091..73600)
                     /locus_tag="KLH46_000320"
     CDS             complement(73091..73600)
                     /locus_tag="KLH46_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBE0377146.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73763..74008
                     /locus_tag="KLH46_000321"
     CDS             73763..74008
                     /locus_tag="KLH46_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0377147.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73983..75614
                     /locus_tag="KLH46_000322"
                     /pseudo
     CDS             73983..75614
                     /locus_tag="KLH46_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18611 a        18992 c        19206 g        18809 t
ORIGIN      
        1 aacataacat cttattggat tagatatgtt aacaattaat tattcttaat atatcaataa
       61 agtatatatt actctcttta ctatctgttt ttattgcggt agcgatacta ataacaaaat
      121 tcggaattta tttagatgct ggaattagaa aaagggatga cggctttctc accttaccct
      181 gaagtttcac gaaagtgtgt aaggaagtat gcaatctaac tggagatttc tgatgatcta
      241 gtcgcttaga gcgacaatga aggtgaccgc atcctgggta gccctatgaa aggatactat
      301 tgaaacgtga tgctaaaaca gtgatgaaat gaataatacg atggaaacgt cttccatgtt
      361 aattatcatg gaagacgttg atgttgaaca cctaaaccag ccccctgata cgaggctggc
      421 ttaagcgcgc aggcaatcag taaaaatcac cggttgcctt ttcggcctga tccatccaca
      481 ccggtttatc gctggttttc gaccagacgc ggtgcaggta gctgtaaaag cgggcgcggt
      541 ctttccagaa aagcatgaaa ggcagcgcca gaacgcccgc tgcaaccgca tatgtacggc
      601 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc tccccctttt
      661 gtatctaccc ggtgaatagc accggaaaaa tgaatttgtt tatctgatga aaatagtacc
      721 gccttttgtg taattttact actcatccga ccacttattt ttgcttattg atggtttatt
      781 tacattcaca ctgtaattaa attacacaaa agttaaatta atactaacca tcagttaaat
      841 catggccttt gccattttta tacttttttt acaccccgtc cgcagatttt tgcgaaatct
      901 ttgcagccag aattctactc ttccggtatc acttttaggc cactggaggt gcactgtgag
      961 tgcaggcgtg atagccggcg tgctgctggt gtttttatta ctgggttatt tggtttatgc
     1021 cctgatcaat gcggaggcgt tctgatggct gcgcaagggt tcttactgat tgccacgttt
     1081 ttactggtat taatggtgct ggcgcgtcct ttaggcagcg ggctggcgcg gctgattaat
     1141 gacattcctc ttccaggtac agcgggcgtt gaacgtatac tctttcgttt acctggcgtc
     1201 tctgaccatg agatgaactg gaagcaatat ctttgtgcca ttctcggcct gaacatgctg
     1261 gggctggcgg tgctgttttt tatgttgctc ggtcagcact atctgccgct taatccacag
     1321 cagttgccgg ggctgtcgtg ggatctggcg ctgaataccg ccgtcagctt tgtcacgaat
     1381 accaactggc aatcttatag cggtgaaacc acgttgagct atttcagcca gatggcaggc
     1441 ttaacggtac aaaactttct ttctgccgcc agcgggattg cggtgatttt tgccttcatc
     1501 cgtgcgttta ctcgccagag catgagcaca ctcgggaatg cctgggtcga tctgctacgc
     1561 atcacgttat gggtgctagt ccctgtggcg ttgttgatcg cactgttttt tattcaacaa
     1621 ggtgcgcagc aaaactttct gccctatcag gcagtgaata ccgttgaagg agcgcaacag
     1681 ctgttaccca tgggaccagt agcttctcag gaagcgatca agatgctcgg tactaacggc
     1741 ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa acccaaccgc actgaccaac
     1801 ttcgtgcaga tgctggcgat cttcttgatc ccaacagcgc tgtgctttgc ctttggtgaa
     1861 gtgacgggcg atcgccgcca ggggcgcatg ttgctgtggg cgatgtcagt gatttttgtc
     1921 atctgcgtag gcgtggttat gtgggcagaa gttcagggta atcctcatct gctggcgctc
     1981 ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc gtttcggcgt gctggtcagt
     2041 agcctgtttg cggtcgtgac tacggcggcg tcctgtggcg cggtgattgc gatgcatgat
     2101 tcgtttaccg ctctcggcgg catggtgccg atgtggctaa tgcaaattgg tgaagtggtg
     2161 ttcggcggtg tcggttctgg tctttacggc atgatgctgt ttgtcctgct ggcggtgttt
     2221 attgccgggc tgatgattgg tcgtacaccg gaatatctgg gtaaaaaaat agacgtacgc
     2281 gagatgaaac tgactgcact ggcgattctg gttactccaa cactggtact gatgggcgcg
     2341 gcgttggcga tgatgaccga cgccggacgt agcgccatgc ttaaccctag cccgcatggt
     2401 tttagcgaag tgctgtacgc cgtgtcgtcc gccgctaaca acaacggcag cgcctttgcc
     2461 ggattaagcg ccaactctcc gttctggaac tgtttactgg cgttctgcat gtttgtcggt
     2521 cgcttcgggg tgattatccc ggtgatggca attgccggtt cgctggtgag taaaaagagc
     2581 caacccgcca gctccggcac gctgccaacg cacggcccgc tgtttgttgg cctgttaatc
     2641 ggcaccgtgt tgctggttgg cgcactgacc tttatccctg ccctggcgct tggtccggtg
     2701 gcggaatatc tctcctgatg atattgagtg agcactgaat atgagtcgta aacaactggc
     2761 gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa gcggtgaaaa aattaaaccc
     2821 gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg atcggcagtc tgctgaccac
     2881 ctgtattagc atcgcgatgg caagcggtgc gatgcctggc aatgcgctgt ttagcgcggc
     2941 aattagcggc tggctgtggg tcaccgtact gttcgctaat ttcgccgagg cgctggcaga
     3001 aggccgcagt aaagcgcagg ccaacagtct taaaggggtg aaaaaaactg cctttgcccg
     3061 caagctgcgt gagccgaaat atggcgctgc ggcggacaaa gttcctgccg atcaactgcg
     3121 taaaggtgac atcgtgctgg tagaagctgg cgatattatc ccctgcgatg gtgaagtcat
     3181 tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg gaatcggcac cggtgatccg
     3241 tgaatccggc ggcgattttg cctccgtcac cggcggcacg cgtattcttt ctgactggct
     3301 ggtgattgag tgtagcgtta accccggtga gacatttctg gatcggatga tcgcgatggt
     3361 ggaaggcgca cagcgacgca aaacaccgaa cgagattgcc ctgactatcc tgctgattgc
     3421 tctgaccatc gtctttttac tggcaaccgc cacgctgtgg ccgttttccg cgtggggcgg
     3481 taatgccgtg agtgtaacgg tactggtggc gctgctggtc tgtctgatcc caaccactat
     3541 tggtggcctg ttgtcagcga tcggcgtcgc cgggatgagt cggatgctgg gcgcgaatgt
     3601 gattgccacc agcggacgtg cagttgaagc ggcaggtgac gttgacgttc tgctactgga
     3661 taaaaccggc accatcacac tcggtaaccg tcaggcgtcg gagtttatcc ccgcgcaggg
     3721 cgtggatgaa aaaacgctgg ctgacgccgc acaactggct tcgctggctg atgaaacgcc
     3781 ggaaggtcgc agtattgtga tcctcgccaa gcaacgtttt aacctgcgcg agcgcgatgt
     3841 gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa agccggatga gcgggatcaa
     3901 catcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc attcgccgcc atattgaggc
     3961 taacggcggt cacttccctg ccgatgttga tcaaaaagtc gatcaggttg cgcgtcaagg
     4021 agccacgctg ctggtggtgg tggaaggttc tcgtgtgctg ggcgttattg cgctgaaaga
     4081 tatcgtcaaa ggcggtatta aagagcgctt cgcccagctg cgcaaatggg cattaaaacg
     4141 gtgatgatta ccggcgataa ccgtctgact gccgccgcga tagctgcgga agcgggtgtc
     4201 gatgattttc tcgccgaagc gacaccggag gccaagctgg cattgatccg tcagtatcag
     4261 gcggaaggtc gtttggtagc gatgaccggc gacagcacca acgatgctac ggcgctggcg
     4321 caggcagatg tcgcggtggc gatgaactcc ggcacccagg cggctaaaga ggcgggcaat
     4381 atggtggatc tcgactctaa cccgaccaag ttgatcgagg tggtgcacat tggcaaacag
     4441 atgctgatga cccgtggctc gctgaccacc ttcagcattg ccaacgatgt ggcgaaatac
     4501 ttcgccatta ttccggcggc gttcgcggca acatatccgc agttaaatgc gctgaacatc
     4561 atgcgcctgc attcgcccga ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt
     4621 atcgtctttt tgattcctct ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct
     4681 gccatgttgc gccgtaactt atggatttac ggtctgggtg ggctgctggt gccgtttatt
     4741 ggtatcaaag tcattgattt actgctaacc gtttgtagtc tggtgtgagg tttaccatga
     4801 gtggattacg tccggcatta tcgacatttc tctttctgtt attgattact ggcggcgttt
     4861 acccgctgct gaccaccgca ctggggcaat ggtggtatcc ctggcaggcc aacggttcgt
     4921 tgattcgtga aggtgatacg gtgcgcggtt cggcattaat cgggcaaaat tttaccggca
     4981 acggctattt tcatggtcgc ccgtcggcaa cagcagaaat gccctataat ccacaggctt
     5041 ctggcgggag caatctggca gtcagtaacc cggagctgga taaacagata gccgcacgcg
     5101 ttgccgcatt acgggccgct aacccggacg ccagcacgaa cgttccggtt gaactggtga
     5161 cggcgtcggc aagcggactg gacaataaca tcaccccgca agcggcggcc tggcagatcc
     5221 cacgcatcgc aaaagcgcgt aatctcagcg ttgaacagct cacgcagctg atcgcaaaat
     5281 acagccaaca accgctggtg aaatatatcg gccagccggt cgtcaacatt gtcgaactca
     5341 atctggcact ggataagctt gatgaataac gaacccttac gtcccgaccc cgaccgtctg
     5401 ctggaacaaa ctgccgcgcc gcatcggggg aagctgaaag ttttcttcag tgcctgtgca
     5461 ggcgtcggga agacctgggc gatgctggca gaagcccagc gactgcgggc gcaagggctg
     5521 gatattgtgg ttggtgtggt agaaacccat gggcggaaag ataccgccgc catgcttgaa
     5581 gggctggctg ttctgcctct gaaacgccag gcgtaccgtg ggcggcatat cagcgagttt
     5641 gatctcgatg ccgccctcgc ccgccgtccg gcgctgatct taatggacga actggcgcac
     5701 agtaatgcgc caggttctcg tcatcctaaa cgctggcagg atatcgaaga actgctggaa
     5761 gctggcattg atgttttcac aaccgtcaac gttcagcatc tggaaagtct gaatgatgtg
     5821 gtcagcggcg tcaccggaat tcaggtacgg gaaaccgtgc ccgatccttt tttcgatgcc
     5881 gccgacgacg tggtgctggt ggacttgccc ccggacgatc tgcgccagcg gctgaaagaa
     5941 ggcaaagtct atattgccgg gcaggcggag cgcgccattg aacatttttt ccgcaaaggc
     6001 aatctgatcg ccctgcgcga actggcactg cgccgtactg ccgatcgcgt tgatgagcaa
     6061 atgcgcgcct ggcgggggca tcctggcgaa gagaaagtct ggcacacgcg cgacgcgatc
     6121 cttttatgca tcggccataa caccggcagc gaaaactggt ccgcgcggcg gcgcggctga
     6181 cgtcacggct ggggagcgtc tggcacgcgg tgtatgttga aacccctgcc ctgcatcgct
     6241 taccggagaa aaaacgtcgg gcaattctca gcgccttacg tctggcgcag gagctgggcg
     6301 cggagacggc aacactttct gatccagcgg aagagaaagc ggtagtgcgt tatgcccgtg
     6361 aacataatct cggcaagatt attctcggtc gcccagcctc gcgccgctgg tggcgtcggg
     6421 aaacatttgc tgaccgactg gcgcgcatcg cccccgatct cgatcaggtg ctggtcgcgc
     6481 ttgatgaacc acccgcccgc acgattaaca acgcgccgga tagccgctca tttaaagaca
     6541 agtggcgggt acagattcag ggatgcgtgg ttgccgccgc gttatgcgcc gttatcacct
     6601 taattgccat gcagtggctg atggcgtttg atgccgccaa cctggtgatg ctgtatctgc
     6661 ttggcgtggt ggtggcgcta ttttatggac gctggccttc ggtggttgcc accgtcatta
     6721 atgtggtgag tttcgatctc ttttttatcg ccccacgcgg cacgctcgcc gtctctgatg
     6781 tgcaatatct gctgaccttc gcggtgatgt taaccgtcgg gctggtgatc gggaacctta
     6841 ctgctggcgt gcgttatcag gcgagggtag cccgttaccg cgagcaacgc acacggcact
     6901 tatatgaaat gtcgaaagct ctggcggtgg gccgcagtcc gcaggacatc gccgccacca
     6961 gcgaacaatt tattgcctcc acgtttcatg cccgcagtca ggtgttgttg cccgatgaca
     7021 acggtaaatt gcagccgtta acacatccgc aaggagttac gccatgggac gatgccatcg
     7081 cgcagtggag ttttgataaa ggcctgcctg cgggcgcggg caccgacacg ttacccggtg
     7141 taccgtacca gattttgccg ctaaaaagcg gcgagaaaac ctacgggctg gtggtggtgg
     7201 agccggggaa tctgcgccag ttgatgatcc cggaacagca gcgcctgctg gagactttta
     7261 cgctgttagt cgccaatgcc cttgagcggc tgacgctaac cgccagcgaa gaacaggcgc
     7321 ggatggcaag cgaacgtgaa cagatccgca acgccctgct ggcagcgctt tcgcatgatt
     7381 tacgcacgcc gcttacggtg ctgtttggtc aggcagaaat cttaacgctc gatctggcaa
     7441 gcgaaggatc accccacgcc cgccaggcca gcgagattcg tcagcatgtg ctgaacacta
     7501 cccgactggt gaataatcta ctggatatgg cgcgaattca gtccggcggc tttaatttga
     7561 agaaagagtg gttaacgctg gaagaagtag tcggcagcgc gctgcaaatg ccggaaccgg
     7621 gtttatcgtc gcccatcaat ctttctctgc cagaaccgct gaccttaatc cacgttgacg
     7681 ggctactctt tgaacgggtg ctgattaatc tgctggagaa cgcggtgaaa tatgcgggtg
     7741 cgcaggccga aattggtatc gatgcccacg ttgagggcga aaatctacaa ctggatgtct
     7801 gggataacgg ccccggtctt ccgccaggcc aggagcagac gatatttgat aagtttgctc
     7861 gcgggaataa agagtcggcg gtgccgggtg tcgggcttgg gttggcaatt tgtcgggcga
     7921 ttgtggatgt acacggcggc accattaccg cgttcaaccg accggaaggt ggtgcctgtt
     7981 ttcgtgttac acttccccag caaaccgctc ctgaacttga agactttcat gaggatatgt
     8041 gacaaacgtt ctgattgttg aagatgaaca ggctattcgt cgctttctgc gcacggcgct
     8101 ggagggcgac gggatgcgcg tctttgaggc cgaaactctg caacgcggct tgctggaagc
     8161 ggcaacccgt aagccagatt tgattattct cgatctcggc ctgcccgatg gtgatgggat
     8221 tgagtttatc cgcgacctgc gccagtggag cccggtgccg gtgattgtgc tttccgcacg
     8281 cagcgaagag agcgacaaga tcgccgcgct ggatgccgga gcggatgatt atctgagtaa
     8341 gccgtttggt attggcgaat tgcaggcccg tctgcgcgtc gctttacgcc gccactccgc
     8401 caccgccgcg cccgatccgc tggtgaaatt ttccggtgtt accgtcgatt tagccgcccg
     8461 cgtgattcac cggggtgagg aagaggtgca tctcacacca attgagttcc gcctgctggc
     8521 ggtgctgctc aacaatgccg gaaaagtact cacccagcgc cagctcctta accaggtgtg
     8581 ggggccaaac gcggtcgaac acagtcacta tttgcgtatt tatatgggac atctgcgaca
     8641 aaaactggaa caggatccgg cccggcctcg ccacttcatt actgaaaccg gtattgggta
     8701 tcggtttatg ctttgaattg ttattcaata aattcgctta aatagtgatt aatttattat
     8761 taaacctgcg ccgaatatta ttatttggca ggtttaattt cttaacgcaa atataaaaca
     8821 cgaaattaca acatttaagt aacaaaacag catctaatat ggcgatattc ataattaatg
     8881 atttattatc tggggtatga tcgttttttg ttgatcttct tcacagatta tagccatttc
     8941 atggatagaa taactctacc ttcaactgac acagcaagcg gtaaaggtaa atggaaaata
     9001 ataaccgctt aatgcctcat ataaggcgga caacccatat catgaagttt gcccatcgta
     9061 atagttttga ctttcatttc tttaatgccc gctagtcttc tgactaaagg gcaccccaac
     9121 gtacaggtct ccctgacttt aagcattaca ggttaatacc tgtattcctc ggtgctcata
     9181 tactgctaac ccttttaaac tctaaataat tcgagtcgca gcacttgcaa cttgaggtat
     9241 gacgagtata gccagttacc gggctggtct gggttattgc atctgcaaaa agcaaactac
     9301 tgatttattt atcagcggtg gagctttgct ttttttccga cgtgatcgat ttctcctttg
     9361 agaaattgag gacctgctat tacctgaaat aaagagatga ataaaatgtc agaattaaaa
     9421 attgccgtta gccgttcttg cccggattgt ttttccactc atcgtgcatg cgtgaatatt
     9481 gacgaaagta attatattga cgttgccgcc attattttat cagtcaatga tgttgaacgt
     9541 ggaaaactcg ataaaataga cgctactggc tatggcattc ctgtttttat tgcaacggaa
     9601 aatgaagaac gtgtaccagc agaatatctg ccacgtattt ctggtgtctt cgagcattgc
     9661 gaatcacgga aagagtttta tggacgtcag ttagaaaccg ctgctagcca ttatgaaacg
     9721 caactgcgcc cgcccttctt ccgcgcactg gtcgattatg tcaatcaggg caatagcgct
     9781 tttgactgcc cgggtcatca gggaggtgag tttttccgtc gtcatccggc agggaatcag
     9841 ttcgtggagt acttcggaga aacgctgttc cgttccgact tatgcaacgc agacgtagca
     9901 atgggcgatc tgctgattca cgaaggcgcg ccatgcattg cacagcaaca tgcggcaaaa
     9961 gtgtttaatg ccgataaaac atacttcgtt ctaaatggca cttcatcttc taacaaagtg
    10021 gttttaaacg ccctgctaac accgggtgat ctggtgctgt ttgatcgcaa taaccacaaa
    10081 tctaaccacc acggagcgtt gctacaggct ggtgcaacac cggtttatct ggaaacggca
    10141 cgtaacccgt atggctttat cggtggcatt gatgcgcact gttttgaaga aagttacctg
    10201 cgtgagctga tcgcggaagt cgcaccgcag cgggcaaaag aggctcgtcc tttccgcctc
    10261 gctgtgattc agttaggcac ctacgacggt acgatttata acgcccgcca ggtggtggat
    10321 aaaattggtc atctgtgtga ctacattctg tttgactccg catgggttgg ctatgagcaa
    10381 tttatcccga tgatggctga ctgttcgccg ctgttgctgg atcttaatga gaacgatccg
    10441 ggtattctag ttacgcaatc cgtacataaa cagcaggctg gtttttctca aacttcacaa
    10501 atccataaaa aagacagcca catcaaaggg caacagcgtt atgtaccgca caaacgcatg
    10561 aacaacgcct ttatgatgca cgcctccacc agcccgttct atccgctgtt tgccgcactg
    10621 gatatcaacg ccaaaatgca tgaaggtgtc agcggtcgta atatgtggat ggattgtgtg
    10681 gtaaatggca ttaatgcccg caaactgatc ctcgataact gtcagcatat tcgtccgttc
    10741 gtacctgaac tggtggatgg taaaccctgg cagtcgtatg aaacagcgca aattgcggtt
    10801 gatctgcgct tcttccagtt tgtaccaggg gaacactggc attcttttga aggctatgca
    10861 gagaatcaat actttgtcga tccttgcaaa ctgttgctga caaccccagg tattgatgca
    10921 cgtaacggcg aatatgaagc gttcggtgta cccgcgacga ttcttgctaa cttcctgcgc
    10981 gaaaatggcg tagtgccgga aaaatgcgat cttaactcca tcctcttcct gctgactccg
    11041 gcagaagata tggccaaact tcagcaactt gtgccctgct ggtacgcttc gaaaaactgc
    11101 ttgagtccga cgcgccatta gcagaagtgc tgccttccat ctacaaacag catgaagagc
    11161 gctacgccgg ttataccctg cgtcagttgt gccaggaaat gcatgatttg tatgcccgcc
    11221 acaacgtgaa acaactgcaa aaagagatgt tccgcaaagc gcacttccca cacgtcagca
    11281 tgaatccgca agaagccaac tacgcctatt tacgcggtga agtggagctg gttcgtctgc
    11341 cggatgccga aggtcgtatc gctgccgaag gggcgcttcc ttatcctccg ggtgtgctgt
    11401 gtgttgttcc gggtgaaatc tggggtggtg ctgttttgcg ttacttcagc gctctggaag
    11461 aagggatcaa cctgctgcca ggttttgcac cggagctgca gggtgtctat atcgaagaac
    11521 atgatggtcg taagcaagtt tggtgctatg tcatcaagcc tcgtgatgcg caaagcaccc
    11581 tgttgaaagg ggaaaaatta tgagtcaggc taaatcgaac aaaatgggcg tcgttcagtt
    11641 aaccatactg acgatggtca acatgatggg ctccggtatc atcatgctgc cgacaaagct
    11701 tgccgaagtt gggacaattt caattatctc ctggctggtg acagccgtag gctcaatggc
    11761 actggcatgg gcattcgcaa aatgcggtat gttcagccgt aaatcaggtg gtatgggcgg
    11821 ttatgccgag tatgctttcg gtaaatccgg taactttatt gcgaactata cctacggcgt
    11881 ctcactgctg attgctaacg tcgcgattgc tatttcggcg gttggttacg gcaccgaact
    11941 gctcggcgcg agtttgtcgc cagtgcagat tggtcttgcg accatcgggg tgctgtggat
    12001 ttgtaccgtg gctaactttg gtggtgcgcg cattaccggg caaatcagta gcattaccgt
    12061 gtgggggggc attattccgg tggtaggcct gtgcattatc ggctggttct ggtttagccc
    12121 gacgctttac gttgattcct ggaatccgca tcatgcaccg ttcttcagtg cagtaggttc
    12181 ttccatcgct atgacgctgt gggcttttct tggtctggag tctgcgtgtg cgaatactga
    12241 tgtagtggaa aacccggaac gtaatgtgcc aatcgcggta ctcggcggta cgttaggtgc
    12301 ggcggtgatt tatatcgtct ccaccaacgt gattgcaggg attgtgccta atatggagct
    12361 ggcaaattca acggcaccat ttggcctggc cttcgcacag atgtttacac cagaagtggg
    12421 taaagtcatt atggcgctga tggtgatgtc ctgctgcggt tcgctgcttg gctggcagtt
    12481 caccattgcc caggtgttta aatcttcatc tgatgaaggc tacttcccta aaattttctc
    12541 ccgtgtaacc aaagtggatg caccggtgca gggaatgttg accattgtga ttatccagag
    12601 cgggttggcg ctgatgacga ttagcccgtc gctaaacagt cagttcaacg tgctggttaa
    12661 cctggccgtg gtgaccaata tcattccgta tattctgtca atggcggcat tagtcattat
    12721 tcagaaggtc gctaatgtgc ccccctcaaa agcgaaagtt gcaaactttg ttgcttttgt
    12781 tggcgcgatg tatagctttt atgcgctgta ctcatccggg gaagaagcca tgctgtacgg
    12841 ttccatcgtg accttccttg gttggacact gtatggtctg gtatcaccac gctttgaact
    12901 gaaaaataaa cacggttaaa aaagcaaagt aaaaaagggc gatcttgcga ccgctctttt
    12961 tttattaaat gtgtttacgc gtttttcaga acttcgctaa caatctcaac cgcttctttc
    13021 tcaatctgct tgcgatgttc ttcaccgagg aagctttcgc agtagatctt atatgcgtct
    13081 tccgtgcctg acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt cagaccgcca
    13141 atagaagcac cgttgcccgg agcagcagtc aggcgcgcgg tgatcgggtc acctgccagg
    13201 gtgctggcgc tcaccatttc cggagacagc ttagacagcg ccgctttttg tgcggaagtc
    13261 gcagctgcct gcaaacggtt gtagctcggc gcaccaaagc gttttgccag ttcgttgtag
    13321 tgttcctgcg ggttcttacc ggtgacagcg gtgatttccg ccgccagcag acacataatg
    13381 atgccgtctt tgtcggtgga ccacggcgtg ccgtcgaaac gcaggaagga agcccctgcg
    13441 ctctcttcgc cgccaaagcc gaagctgccg tcgaacagac catcaacaaa ccatttgaaa
    13501 cctaccggta cttctaccag cttacggccc aagtcattga ccacacggtc gatcatcgca
    13561 gaagaaacca gcgttttacc gacagcaaca tctttgcccc actgcggacg atgctggaac
    13621 aggtagttga tcgccaccgc caggtagtgg ttcggattca tcaaacctgc cggagtgacg
    13681 ataccgtgac ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt atcacgcagt
    13741 gccagcagac ccgccatcgc acactcggag gagcagtcca tacggatcgc accgtcttta
    13801 tcgaggtgca taaagcggaa ggtttgatcg acctgatcgt taacgatggt caggttgagg
    13861 ttgtaatact cgccaatgcg cttccagtat tcgataccgg aaccgcccaa cggatcaacg
    13921 cccagcgtca ggcccgcttt ctgaatcgcc gccatatcaa cgatatcggc cagcccttcc
    13981 acgaacggct gcaccagatc ctgctctttc acatagccgg atgccatcgc ttcgtcgagg
    14041 gagatacgct tcacgccttt caggccatcg gccatcagtg cgttggccct gtcttccacc
    14101 actttggtga cgttagtatc agccgggcca ccatttggcg gattgtactt gataccacca
    14161 tcttccggcg ggttatggga cggtgtaatc acgataccgt ctgccagcgg gccacctttt
    14221 ttattgtgaa ccaggatggc attggaaacg gcaggcgtcg gagtgaagcc attgttttcc
    14281 tgcacaatga catcaacgcc gttcgctgcc agcacttcca gtacggaaat gaacgcaggc
    14341 tcggacaggg cgtgagtatc tttacccaca tagcaagggc cagtgatgcc gtttttcgca
    14401 cgttcttcag caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa gctgtggcgc
    14461 gctgcactgc cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc attccctgct
    14521 tctggtttca gtacataata ttgcgccgtc agttgggcga cgttaatcaa atcactctgt
    14581 tgtgcaggtt ggcctgcacg attgtggatt gccattgctt tgtcctttgt ctgcaacgtt
    14641 ttagatagtt ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg actgcatgat
    14701 gtgttcgatc atgctgcatt tacggccggt gttggtgttg gtgatcaccc aatacggcgt
    14761 gcctggcaca tgtttcggct tggtctgatt accatttttc agcagcgttt gttcatctgc
    14821 cgcaaagtac acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa acgcctgggc
    14881 gtcaagagaa tatagtgtag acaacagcag cataaagcga ttgaccgctc gcttttgctc
    14941 tgcgtattcg tccgaaagca gaagttcacg cattgcgcga accttgtctt taatcgtttt
    15001 gaccggcttc gcttcgacga tagcaggtga cgcaacgcga acctctttcg tcaccggagc
    15061 agcaggctgt gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg atgcgctctc
    15121 gccgatatgc ttagtgtggc tggcaatata gctgtagagt tcatcatcaa cttcaatcgt
    15181 tttcatctta atccagtgca gtgtcttagc tgaatacaag tcgccaagag tatatgaata
    15241 aatcccggca gcggatagcg tcaagcctgg cttgcagaaa aactcggaat aaaccttatt
    15301 tgttgcagcc ctgtggcaca atggtcaaca taatacccta acccgacaac gcgaaaaaaa
    15361 gaactttgcc atgaaattaa atatccgcgc gcaaactgca caaaaccagc acaataattc
    15421 tcccatcgtt cttgtccatg gtctgtttgg tagcctcgac aaccttggcg tactggctcg
    15481 cgatctggta aacgatcaca atatcatcca ggttgatatg cgtaaccacg gtctttcacc
    15541 gagagagccg gtaatgaatt acccggcgat ggcgcaggat cttgttgata ccctggatgc
    15601 actgcagatc gacaaagcaa catttatcgg tcactccatg ggcggtaaag cggtaatggc
    15661 acttactgca ctggcccccg atcgcatcga taaactggtg gcgatcgata tcgcaccggt
    15721 cgactatcac gtacgccgtc atgatgagat tttcgcagct atcaacgcgg tcagtgaatc
    15781 ggacgcacaa actcgccagc aagcagcagc aataatgcgc cagcatctta atgaagaagg
    15841 ggtgattcag tttctgttga aatcttttgt tgacggggag tggcgcttta acgtgccggt
    15901 attgtgggat cagtatccgc atattgtggg ttgggagaaa atcccggcat gggatcaccc
    15961 tgccctgttt atccctggcg gcaattctcc gtatgttagc gagcagtacc gtgatgattt
    16021 actggcacaa tttccacagg cacgggcgca tgtgattgca ggcgcgggtc actgggtcca
    16081 tgctgaaaaa ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg attaacgcaa
    16141 ctgattaaaa acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg tgactcatcg
    16201 cgctgatgta ttatggcgcg ctatccatcc gggcagaggc ctggctgatt gtttcccccg
    16261 aagtcaccaa gatcatggcc aaagaacaaa cggaccgtac gacattagat ctgttcgcgc
    16321 acgagcgtcg accgggacga ccgaaaacta atccgctttc gcgcgatgaa cagctgcgta
    16381 ttaataaacg caaccagcta aaacgcgaca aagtacgtgg ccttaagcgt gtcgaactga
    16441 agctgaacgc ggaagctgtc gaggcgctga acgagctggc ggaatcgcgt aatatgagcc
    16501 gtagcgaact gatcgaagag atgttaatgc agcaactggc ggcgctgcgt agtcagggca
    16561 tcgtttaaat ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt gctgattccg
    16621 caagactgcc tgttctgcta tgattgcctt tatccgtggg caattttcca cccccatttc
    16681 aataagtttc aagaggttat ttcactcatg gctatcactg gcatcttttt cggcagcgac
    16741 accggtaata ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa agacgttgcc
    16801 gatgtccatg acattgcaaa aagcagcaaa gaagatctgg aagcttacga cattctgctg
    16861 ctgggcatcc caacctggta ttacggcgaa gcgcagtgtg actgggatga cttcttcccg
    16921 actctcgaag agattgattt caatggtaaa ctggttgcgc tgtttggttg tggtgaccag
    16981 gaagattacg ccgaatattt ctgcgacgca ttgggcacca tccgcgacat cattgaaccg
    17041 cgcggtgcaa ccatcgttgg tcactggcca actgcgggct atcatttcga agcatcaaaa
    17101 ggtctggcag atgacgacca ctttgtcggt ctggctatcg acgaagaccg tcagccggaa
    17161 ctgaccgctg aacgtgtaga aaaatgggtt aaacagattt ctgaagagtt gcatctcgac
    17221 gaaattctta atgcctgatg tgatgcggcg tagactcatg tctacgccgt attaatagat
    17281 aatgccaatc aaaataattg ctacaaattt gtaacttttg ctgttgtacc tgtacaatgt
    17341 cccagtgttc aagtggcctt gccgttgtaa atgtaagctg tgccacgttt ttattaacaa
    17401 tatttgccag ggacttgtgg ttttcattta ggcgtggcaa ttctataatg atacgcatta
    17461 tctcaagagc aaattctgtc acttcttcta atgaagtgaa ccgcttagta acaggacaga
    17521 ttccgcatga ctgataacaa taccgcccta aagaaagctg gcctgaaagt aacgcttcct
    17581 cgtttaaaaa tcctggaagt tcttcaggag ccggacaacc atcacgtcag tgcggaagat
    17641 ttatacaaac gtctgatcga tatgggtgaa gaaattggtc tggctacggt atatcgcgta
    17701 ctgaaccagt ttgacgacgc tggtatcgtc acccgccaca attttgaagg cggtaaatcc
    17761 gtatttgaac tgacacagca acatcaccac gatcacctga tttgcctcga ctgcggcaag
    17821 gttatcgaat ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc cgcaaaacat
    17881 ggcattcgcc tgactaacca cagtctctat ctttacggtc actgtgccga aggcgattgc
    17941 cgcgaagatg agcacgcgca cgaaggcaaa taagccagcc tgaacgagaa aagccaacct
    18001 gcgggttggc ttttttatac aagggaaata agaaaagcga gagttacagc tctcacttat
    18061 ttgttattac tgcgtatttc ctgccagacc ttatcacaat cggctttcac cgcctgatca
    18121 tttccggttt gcgttgcacg cagacactgc tgataatcca gtacgcgtac actttcctgg
    18181 tcggcaaact gctgatgttg tttctctttc ttcagcacgt ttaacacgct ctggcaggct
    18241 tcaatttttt ctggcgaacc ttgcgcggtg ttgatacagg cgctgtatgc ctctttcaga
    18301 cggctgtctt cctgtggtgc ggttgattgc gcacaagcta ccagccccga tgccatcatg
    18361 gcgatgagaa tcagtttttt cattgaatgc ggcaggcaca aaacctgccg cgtcgggcat
    18421 cagaagatgg tgaatggtgc gattaccata aatttcacgt cacgctcatc ctggaagatg
    18481 ttaccgtaac cgccgcccca gcttgggatg tcggagtggt tgtcgtattc ggtgaagtgc
    18541 agtttgaaca tcgtgccttt ggcgcgaccg tcctgaatgg tgtagaccgc atccaggctg
    18601 taggcagact cttcaatagt ccggtttttg tcgtagtacg catccggatt gctctgccag
    18661 gtcgcaggtt tagcatccca tgcgtaaacg taggaagcgc cgatggcgaa gcctggaaga
    18721 ttccagtttt tcaggtcata catcgcactg aagaagaccg ctttttcgcc gttagcgttg
    18781 aagtcggaac ggttatccca ccagatatcc aggcggccgt tggaggaagc gtaggttggg
    18841 gtcatacgtt gcaggaagta tccctgctga ctgtcagcct taatccaggt gccttcgagg
    18901 cgcaaatcca ctacgtcagc cgcccggtaa ccaaaggtca acgcttgcag ccaggcggtg
    18961 ccgtcataaa gatcgttgac gctgcgatcg tcaactttat cgcgggtacc atagaactgg
    19021 tagctggtgg ttaacgggct accggcgata tcaaatttgt agctggcttt ggcaaaatat
    19081 tgatcgatat acccttccgc ctgaccaaat gccgcttcca gtacgaagtt atttttgaag
    19141 tcgtatttcg caccgatgga gtgcagataa tcaactttgg tggttttatc gttctgataa
    19201 aactcatcca tttccagatg ccacggcgct ttgtattcgt tggtccacat gtaggagaaa
    19261 ctcaaggcac cagcatcgcc gtaatcaaaa ttcgccccgg cttccgcccc ctgataagta
    19321 cccggcataa agctccagtg cggcgctaac agcgtttgac cggttggctg aatgtaacct
    19381 gccctcgccc aaaccggacc gtatttaaat ttggccgcag ctttatacag gcttataccg
    19441 cttttgtcgc cggaccagtc ttcgtcatag gctttattac tttttgaaaa cgcgatttcg
    19501 ttcgggtggg agctgtcgcc gttttccgcc atttcaatcg ccgtaaacgc agcaatatca
    19561 aggccgaaca tatcagcagc ataaccggac tgaaaatcga ggttggcatt ccaggtggag
    19621 tgagaaaggt tggttttgta tttgtcgccg tcggtaacat ctttacggtc gcgttcacgc
    19681 tgccagtaat agataccgcc ggttaaggtt gaatcgtcga tgaatccttc ggcgcgagcc
    19741 tccggcattg ccataaagcc cgacattgct gtaacaccgg caatagccag cgccagcgta
    19801 ctacgtttgc cactaaacgt acgcatgggt taatcctctt tgacgtataa attgctgcac
    19861 caaaggtgaa cagcgaaaaa aaataaaaat taaaaaagta gcgttgagaa aagtctcgct
    19921 gactacagga acagactatt tttcgcgacg cgaattatca atctctttct gtaaccagaa
    19981 tgtaagaaca tgagcaaccg cacatatttt gatgattttt gttacaaggc ttaagtttgc
    20041 ggttgaaagg cttgcagaag aataaaaacg tgaaattcca gcaagatgaa agcggttaca
    20101 ttttatttaa ttatgttttg tgattttaaa tttattttat ggataaattg cgaagcggaa
    20161 ttaattcgcg aagaaaagca gaaaaaacgc cgcagagcgc ggcgtttggg attgtttgct
    20221 atttcttgcc ggatgcggcg tgaacgcctt atccggccta caaaagcacg caaattcaat
    20281 atattgcaga gatcatgtag gcctgataag cgaagcgcat caggcaattt agcgtttgaa
    20341 atttattgtc tatttattgc cggatgcgac gtaaatgcct cacccggctt ggtagaactt
    20401 aaaattactc gcctactttc gcccaggtat cacgcagccc aacggtgcgg ttaaataccg
    20461 gtttttcggc cgtggaatgg cggctgtcga ggcagaagta accttcacgc tcaaactgga
    20521 atgctttacc cgcaaccgca tctttcagcg acggttcagc aaagccctgt ttgatcacca
    20581 gcgattccgg gttaatcacc gacaggaaat catccgcagc acctgggtta ggcacgctga
    20641 acagacgatc atacaaacgg atttcaaccg gcagcgcatg tgccgcgctc acccagtgaa
    20701 taacaccttt gactttacga ccatctgccg gatctttgct taaggtgtcg gcgtcataag
    20761 tacagaagat ggtggtgata ttaccttcgg catctttctc cacgcgttca gccttaatga
    20821 cgtaagcatt acgcagacgc acttctttac ccagcaccag acgtttgtac tgcttgttag
    20881 cttcttcgcg gaaatcggcg cgatcgatcc aaatttcacc gctaaacggc acctgacggc
    20941 tccccatttc cggtttgttc ggatggttcg gcatggtgac catttcgcct tcgccctgat
    21001 agttttcgat aaccagtttc accggatcga taaccgccat tgcgcgcggc gcattttcgt
    21061 tgagatcttc acggatgcag gattccagcg acgccatctc aatggtgttg tcctgcttgg
    21121 tcacgccgat gcgtttgcag aactcacgaa tagaagccgc agtgtaacca cgacgacgca
    21181 gaccggaaat ggtcggcata cgcgggtcat cccagccttc aacgtgcttg tcggtcacca
    21241 gcaggttcag cttacgcttg gacatcacag tgtattccag attcaggcgc gagaattcgt
    21301 actggcgcgg atgaacagga atggtgatgt tatccagtac ccagtcgtac agacgacggt
    21361 tgtcctggaa ctcaagcgta cacagagagt gcgtaatacc ttccagcgca tcgctgatgc
    21421 aatgggtgaa gtcgtacatc gggtagatgc accacttgtt gccagtctgg tggtgttcag
    21481 caaatttaat gcggtacagc accggatcgc gcattacgat aaatggcgac gccatgtcga
    21541 ttttcgcacg caggcaggct ttaccttctt caaaaccacc ggcacgcatt ttttcgaaca
    21601 gcgccaagtt ctcttcaacg ctgcggtcgc ggtacgggct gtttttaccc ggctgcgtca
    21661 gggtaccgcg gtattcgcgg atctgttccg gtgtcagttc atcaacgtac gccaggcctt
    21721 tattgatcag ttcgatcgca taggcgtgga gctgatcaaa ataatcggag gagtaacgga
    21781 cgttaccaga ccagtgaaaa cctaaccact ctacgtcgtt tttgatcgaa tcgacatact
    21841 cgatatcttc ttttaccggg ttagtgtcgt cgaaacgcag gttgcactgg cctttatagt
    21901 cctgggcgat cccgaagttc aggcagatag atttcgcatg gccaatatgc agatagccat
    21961 tcggctccgg cgggaaacgg gtgtgtactg tgatgtgctt accactggcc agatcttcat
    22021 cgatgatctg acggataaag ttagtcgggc gggcttctgc ctcactcatc gtggattcct
    22081 caaagcgtaa acaacgtata acggcgtatg atcttataag cgggacaggc tgacaactgt
    22141 tagtttttta aaattgataa tctaacaagg attatgggga tgattgatga aaaaaaaagc
    22201 ggcagagaag atctccaccg cttgtcattg ttggatgcga cgctgccgtg tcgcatcagg
    22261 cataaagcag attacttttt gatttcatac agcggtgttt gacccgccac aacatggccc
    22321 tgagctttaa tgagcaagcc actgaaatcg tcgatattgc tgcaaaccac cgggctaatc
    22381 atcgagcggg cgttagcgtt caggtaatcc agatccattt ccagaatcgg ttgccctgcg
    22441 cttacctgcg caccctcttc caccagacgt ttaaagcctt taccttccag cgctacggtg
    22501 tcgataccca tatggacgac gatctccgcg cctttttcgg tttccaggca gaacgcgtgg
    22561 ttggtgttga agattttcac gattgtccct gcggctggtg atacgacgat tttatctgtc
    22621 ggtttcaccg ccacaccgtc acccaccgct ttgctggcga atgcttcgtc aggaacctga
    22681 tccagtgcca cgacatcacc ggtaatcggc gataccagct ccgcgataga taccgcgttt
    22741 ggtacagcct gcggttttgc tacaggcgcg gcagttgccg gagttgcttc agctgacgca
    22801 gcggctaccg gaccacgggc aacgactttc ttcatcgcat cgccgatgga ttctgctttc
    22861 gcgccaacaa tcacctgaat agtctgtttg ttcagtttca ctacaccaga agcacccaga
    22921 cgtttacaca tcgtatcgtt gacgcgggca gagtcagcca cggtcaggcg cagacgggta
    22981 atacaggcat caatcgcttt caggttgtca gtaccgccaa ccgcagcaat atagttggtt
    23041 gccagttgag tcagaccttc ttcagtattg ctgttggctt cttcagtaac gatctcgtct
    23101 tctttatctt cacgacccgg cgttttcagg ttgaacatgc ggataaccaa actgaacacc
    23161 acgaagtaga tagcgaagaa gataacgccc atcaccagca gcatccagac gttctggctg
    23221 gcggcaggca ggttatacat caacgcgtag tcgatagcac ccgcagagaa agagaagccg
    23281 gcatggatac ccagcagcgt tgccacaaac aggctgatac cggtcagcag tgcgtgcagg
    23341 aggtacagca gcggcgcaag gaacatgaac aggaattcca gcggctcagt cacaccggtc
    23401 aggaacgcag taacagcaac agaaagcagc atcccgccaa ccatcggacg acgctctttc
    23461 ggtgctgcga agtacatcgc cagcgccgca cccggcagac cgaacatcat gatcgggaag
    23521 aagccggaca tgaacatccc cgcggtgccg tcaccagcgt agaaacggtt gatgtcgccg
    23581 tggaaaaccg tacccgccgc gttggtgaat tcaccaatct ggaaccaggc gatggtgttc
    23641 agcacctgat gcagaccggt tgggatcagc agacggttga tgaaaccaaa gataccggaa
    23701 cccagcgcgc ccgcagaaac gatccactcg ccgcctgcat ggatagcgtg ctgtaccggc
    23761 ggccagacgt aaccaaaaat ggccgccagc accagacaga agaagccggt ggcgatcggc
    23821 acaaagcgtt tgccgccgaa gaagctcagg aagtccggca gtttaatatc ggaccaacgg
    23881 ttataggctg cgccaccaac cagaccggta atgatacccg ccagtacacc catgttaatt
    23941 tctgggttga tggtcaccat cgctttggtt aacacaaagt aacctaccgc acccgccagc
    24001 gccgccgcac ctgcgctgtc tttcgaccag ctggatgcca caccgatggc gaagattaat
    24061 gcgaggttat caaaaattgc accgcccgcc tgggcaataa acgcaacgtt aagtaaatct
    24121 ggctgaccga atcgcagcaa cagcgccgcc accggcagca ccgcgatagg gagctgtaac
    24181 gccctaccga gtcgctggaa aaaacctaaa atattcatct tattccccct acgagaaccc
    24241 tatttggctc gtttaaagcc gtatttttat tttgctgcaa attttactgc cgatgttctg
    24301 taatcagatt gttagatcat ctgctacaga gtgtgtgaaa atttaattcg catcgcaaat
    24361 taaacgcgtg tcttttgtga gttttgtcac caaatatcgt tatcatcact cccttttact
    24421 ggctaaacca gaaaacttat tttatcattc aaaaaatcaa gtcggattga cgcctgtctg
    24481 cgcaaatcca ggttacgctt aaagatgtct aatccgccaa cggcttacat tttacttatt
    24541 gaggtgaata atgagactga tccccctgac taccgctgaa caggtcggca aatgggctgc
    24601 tcgccatatc gtcaatcgta tcaatgcgtt caaaccaact gccgatcgtc cgtttgtact
    24661 gggcctgccg actggcggca cgccgatgac cacctataaa gcgttagtcg aaatgcataa
    24721 agcaggccag gtcagcttta agcacgttgt caccttcaac atggacgaat atgtcggtct
    24781 gccgaaagag catccggaaa gctactacag ctttatgcac cgtaatttct tcgatcacgt
    24841 tgatattcca gcagaaaaca tcaaccttct caacggcaat gccccggata tcgatgccga
    24901 gtgccgccag tatgaagaaa aaatccgttc ttacggaaaa attcatctgt ttatgggcgg
    24961 tgtaggtaac gacggtcata ttgcatttaa cgaaccggcg tcttctctgg cttctcgtac
    25021 tcgtatcaaa accctgactc atgacactcg cgtcgcgaac tctcgtttct ttgataacga
    25081 tgtcaatcag gtgccaaaat atgccctgac tgtcggtgtt ggtacactgc tggatgccga
    25141 agaagtgatg attatggtgc tgggtagcca gaaagcactg gcgctgcagg ccgccgttga
    25201 aggttgcgtg aaccatatgt ggaccatcag ctgtctgcaa ctgcatccga aagcgatcat
    25261 ggtgtgcgat gaaccttcca ccatggagct gaaagttaag actttaagat atttcaatga
    25321 attagaagca gaaaatatca aaggtctgta attgttatcc ctgccctggt tccttgctca
    25381 gggcaatatt ttttaaaatc gggggtcaga atgtatgcat taacccaggg ccggatcttt
    25441 accggccacg aatttcttga tgaccacgcg gttgttatcg ctgatggcct gattaaaagc
    25501 gtctgtccgg tagcggaact gccgccagag atcgaacaac gttcactgaa cggggccatt
    25561 ctctcccccg gttttatcga tgtgcagtta aacggctgcg gcggcgtaca gtttaacgac
    25621 accgctgaag cggtcagcgt ggaaacgctg gaaatcatgc agaaagccaa tgagaaatca
    25681 ggctgtacta actatctgcc gacgcttatc accaccagcg atgagctgat gaaacagggc
    25741 gtgcgcgtta tgcgtgagta cctggcaaaa catccgaatc aggcgttagg tctgcatctg
    25801 gaaggtccgt ggctgaatct ggtaaaaaaa ggcacccata atccgaattt tgtgcgtaag
    25861 cctgatgccg cgctggtcga tttcctgtgt gaaaacgccg acgtcattac caaagtgacc
    25921 ctggcaccgg aaatggttcc tgcggaagtc atcagcaaac tggcaaatgc cgggattgtg
    25981 gtttctgccg gtcactccaa cgcgacgttg aaagaagcaa aagccggttt ccgcgcgggg
    26041 attacctttg ccacccatct gtacaacgcg atgccgtata ttaccggtcg tgaaccgggc
    26101 ctggcgggcg cgatcctcga cgaagctgac atttattgcg gtattatcgc tgatggcctg
    26161 catgttgatt acgccaacat tcgtaacgct aaacgcctta aaggcgacaa actgtgtctg
    26221 gttaccgacg ccaccgcgcc agcaggtgcc aacattgaac agttcatttt tgcgggtaaa
    26281 acaatatact accgtaacgg actttgtgtg gatgagaacg gtacgttaag cggttcatcc
    26341 ttaaccatga ttgaaggcgt gcgtaatctg gtcgaacatt gcggtatcgc actggatgaa
    26401 gtgctgcgta tggcgacgct ctatccggcg cgtgcgattg acgttgaaaa acgtctcggc
    26461 acgctcgccg caggtaaagt agccaacctg accgcattca cacctgattt taaaatcacc
    26521 aagaccatcg ttaacggtaa cgaggtcgta actcaataag agaaagtatg acaccaggcg
    26581 gacaagctca gataggtaat gttgatctcg taaaacagct taacagcgcg gcggtttatc
    26641 gcctgattga ccagtacggg ccaatctcgc ggattcagat tgccgagcaa agccagcttg
    26701 cccccgccag cgtaaccaaa attacgcgtc agcttatcga acgcgggctg atcaaagaag
    26761 ttgatcagca ggcctccacc gggggccgcc gcgctatctc catcgtcacc gaaacccgca
    26821 atttccacgc aatcggcgta cggcttggtc gtcatgacgc caccatcact ctgtttgatc
    26881 tcagcagcaa agtgctggca gaagaacatt acccgctgcc ggaacgtacc cagcagacgc
    26941 tggaacatgc cctgctgaat gccattgctc agtttattga tagctaccag cgcaagctgc
    27001 gcgagctaat cgccatttcg gtgatcctgc cagggcttgt tgacccggac agcggcaaaa
    27061 ttcattacat gccgcatatt caggtagaaa actgggggct ggtagaagca ctggaagaac
    27121 gttttaaagt gacctgtttc gttggtcacg atatccgtag tctggcgctg gcggagcact
    27181 acttcggtgc aagtcaggat tgcgaagact ccattctggt gcgtgtccat cgcggaaccg
    27241 gggccgggat tatctctaac gggcgcattt ttattggccg caacggtaac gtcggtgaaa
    27301 ttggccatat tcaggtcgaa ccgctgggtg aacgctgcca ctgcggcaac tttggctgcc
    27361 tggaaactat cgccgccaac gctgccattg aacaacgggt gttgaatctg ttaaagcagg
    27421 gctaccagag ccgtgtgcca ctggacgact gcaccatcaa aactatctgc aaagccgcga
    27481 acaaaggcga tagcctggcc tcggaagtga ttgagtatgt cggtcgtcat ctgggtaaaa
    27541 ccatcgccat tgctatcaac ttatttaatc cgcaaaaaat tgttattgct ggtgaaatca
    27601 ccgaagccga taaagtgctg ctccctgcta ttgaaagctg cattaatacc caagcgctga
    27661 aggcgtttcg tactaatctg ccggtggtac gttctgagct ggatcaccgc tcggcaatcg
    27721 gcgcttttgc gctggtaaaa cgcgccatgc tcaacggtat tttgctccag catttgctgg
    27781 aaaattaatg tgcttttata gtggcgctta ttgttgtcaa tattctgggt agtccatgac
    27841 cattaaaaat gtaatttgcg atatcgacgg cgtgctgatg cacgataacg tcgccgtacc
    27901 gggtgcagcg gaatttttgc acgggattat ggataaaggc ctgccgctgg tgttgctgac
    27961 caactatcct tcgcagactg ggcaagatct ggcgaaccgc tttgccactg ccggtgtcga
    28021 tgtgccggat agcgtgtttt atacctctgc gatggcgacc gccgatttcc tgcgtcgcca
    28081 ggaaggcaag aaagcgtatg tggtgggcga aggcgcactg attcatgaac tgtacaaagc
    28141 cggtttcact attaccgatg tgaaccctga tttcgtgatt gttggcgaaa cacgttccta
    28201 caactgggac atgatgcata aagcagccta tttcgtcgct aacggtgcac gctttatcgc
    28261 caccaacccg gacacccacg ggcgcggttt ttatcccgct tgtggcgcgt tgtgtgcagg
    28321 gattgagaaa atctccgggc gcaaaccgtt ctatgttggt aagcccagcc cgtggatcat
    28381 ccgcgcggca ttaaacaaaa tgcaggcgca ttcggaagaa acagtgattg tcggcgataa
    28441 cctgcgtacc gatattctgg cgggcttcca ggccggtctg gagacgattc tggtgctttc
    28501 tggtgtttca tcgctcgacg atatcgacag tatgccgttc cgcccaagct ggatttaccc
    28561 gtcagtcgct gaaatcgacg ttatctgaat accacccacg tctgcggacg tggttggctc
    28621 tgatgacaaa cgccaaactg cctgatgcgc tacgcttatc aggcctacgc agctcctgca
    28681 atatattgaa ttttcatgct tttgtaggcc ggataaggcg ttcacgccgc atccggcatg
    28741 aacaaatcgc actttatcag caatttggcc cccgtaaaat ataaccattc aataaaaccc
    28801 gctaaaaatt atcgattcat caataaatac atcctcacac cagacccttt ttcaccaatc
    28861 gacaatcacc attacgtttt ttatttttgc actgccaaat agcttgcgca cagtgctgct
    28921 ttgcggcatt tttttaaaca agcaaacaca acaagcaaca aataccaggt taacggagaa
    28981 ggttatgtgt tcaatttttg gcgtattcga tatcaaaaca gacgcagttg agctgcgtaa
    29041 gaaagcactc gagctgtcac gcctgatgcg tcatcgtggc ccggactggt ccggtattta
    29101 tgccagcgat aacgccattc tcgctcacga acgtctgtca attgttgacg ttaacgcggg
    29161 ggcgcaacct ctctacaacc aacaaaaaaa ccacgtactg gcggtaaacg gtgaaatcta
    29221 caaccaccag gcattgcgcg ctgaatatgg cgatcgttat cagttccaga cagggtctga
    29281 ctgtgaagtg atcctcgcgc tgtatcagga aaaagggccg gaatttctcg acgacttgca
    29341 gggcatgttt gcctttgccc tgtacgacag cgaaaaagat gcctacctga ttggtcgcga
    29401 ccatctgggg atcatcccac tgtatatggg gtatgacgaa cacggtcagc tgtatgtggc
    29461 ctcagaaatg aaatcgctgg tgccagtttg ccgcacgatt aaagagttcc cggcggggag
    29521 ctatttgtgg agtcaggacg gcgaaatccg ttcttattat catcgcgact ggttcgactt
    29581 tgatgcagtg aaagataacg tgaccgacaa aaacgagctg cgtcaggcac tggaagattc
    29641 cgttaaaagc catctgatgt ctgatgtgcc ttacggtgtg ctactttctg gtggtctgga
    29701 ttcctcaatt atttccgcta tcaccaagaa atacgcagcc cgtcgcgtgg aagatcagga
    29761 acgctctgaa gcctggtggc cgcagttaca ctcctttgct gtaggtctgc cgggttcacc
    29821 ggatcttaaa gcagcccagg aagtggcaaa ccatctgggc acggtgcatc acgaaattca
    29881 cttcactgta caggaaggtc tggatgccat ccgcgacgtg atttaccaca tcgaaactta
    29941 tgatgtgacc actattcgcg cttcaacacc gatgtattta atgtcgcgta agatcaaggc
    30001 gatgggcatt aaaatggtgc tgtccggtga aggttctgat gaagtgtttg gcggttatct
    30061 ttacttccat aaagcgccca acgctaaaga actgcatgaa gagacggtgc gtaaactgct
    30121 ggccttgcat atgtatgact gcgcgcgcgc caacaaagcg atgtcagcct ggggcgtgga
    30181 agcacgcgtt ccgttcctcg acaaaaaatt ccttgatgtg gcgatgcgca ttaacccgca
    30241 ggataaaatg tgcggtaacg gcaaaatgga aaaacacatc ctgcgtgaat gttttgagtc
    30301 atacctgccc gcaagcgtgg cctggcggca gaaagagcag ttctccgatg gcgtcggtta
    30361 cagttggatc gacaccctga aagaagtggc ggcgcagcag gtttctgatc agcaactgga
    30421 aactgcccgc ttccgcttcc cgtacaacac gccgacctca aaagaagcgt atctgtaccg
    30481 cgagatcttt gaagaactgt tcccacttcc gagtgccgct gagtgcgtgc cgggcggtcc
    30541 ttccgtcgcg tgttcttcag ctaaagcgat cgaatgggat gaagcgttca aaaaaatgga
    30601 cgatccgtct ggtcgtgcgg ttggcgttca ccagtcggcg tataagtaag aaatgcggta
    30661 ctgccccgga gaaatccggg gctgttttag ttattgcgct gcttgttgat tctgatatgc
    30721 agtaaggaaa tgttccgggt ttgccacatc actgtttgac aactcatcac tgtaggccag
    30781 tatttcatag attaatttcc caccactatt gttgaattcc gaatttcatc ctgtacgaca
    30841 aaagaatggc tgtagggtgg ggaaaaacac aaagtaaaca ataattgacg aatatagcgc
    30901 cacgctgttc gcaacctaac caaacagtca ctttcgagca attttccttg aaaaagaggt
    30961 tgacgctgca aggctctata cgcataatgc gccccgcaac gccgataagg tatcgcgaaa
    31021 aaaagatggc tacgtagctc agttggttag agcacatcac tcataatgat ggggtcacag
    31081 gttcgaatcc cgtcgtagcc accatctttt ttgcgggagt ggcgaaattg gtagacgcac
    31141 cagatttagg ttctggcgcc gcaaggtgtg cgagttcaag tctcgcctcc cgcaccattc
    31201 accagaaagc gttgtacgga tggggtatcg ccaagcggta aggcaccggt ttttgatacc
    31261 ggcattccct ggttcgaatc caggtacccc agccatcttc ttcgagtaag cggttcaccg
    31321 cccggttatt ggggtatcgc caagcggtaa ggcaccggtt tttgataccg gcattccctg
    31381 gttcgaatcc aggtacccca gccatcgaag aaataatctg gctacgtagc tcagttggtt
    31441 agagcacatc actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccaaatt
    31501 ctgaatgtgt cgaatacgtt cggcaaattc aaaaaccaat ttgttggggt atcgccaagc
    31561 ggtaaggcac cggattctga ttccggcatt ccgaggttcg aatcctcgta ccccagccaa
    31621 tttattcaag acgcttacat cgtaagtgca cccagttggg gtatcgccaa gcggtaaggc
    31681 accggattct gattccggca ttccgaggtt cgaatcctcg taccccagcc acattaaaaa
    31741 agctcgcttc ggcgagcttt ttgcttttct gcgttcattc aatgtcgaat gcgatgttga
    31801 cacgtcttat ccttcaatgt cggatgcgac gctgccgcgt cttatccgac ctacggctgg
    31861 cacgcatccg gcaatgttgt aaggctacaa ccctaacgca tatttcagcg cctgacgttt
    31921 caacacgcca gcacgctctg ccgccattaa cccgagatta cgcacaaaac gcagcggcgg
    31981 cagattatta ctgaatccgg cataaaacag atccatacca ctttgcataa tgaagttatc
    32041 cgccatgcgc cgcatctggt aacgcttgag gacaggataa ctggcccacg cttcaccgta
    32101 gctgcgggca ttcaccagaa catcaatcag ggcatcgaca tcacgataac caagattcac
    32161 cccctgcccc gccagcggat ggatggtatg cgcggcatcg cccaccagcg caagccccgg
    32221 ctgcacatac tgcaacgcat gtcggcgcgt cagcggaaac gcaccagcgg caagcggtgt
    32281 aacgtaaccc agacgcgacg ggaaatgctt cgcgatttcc gcctggagtt gcgccatatt
    32341 catattctgc aactggcgaa tacgcgccgg agagtcatac cacaccagcg atgcccagtt
    32401 atcaaacaac ggcagaaacg cacgcggtcc gtccggggta aattgctgcc aggtgctgtc
    32461 gccgggatcg ttctcacact gtacgctaat caacatgcac gactgcgcat actgccaggc
    32521 atgaacgcca attcccgcca tctgccgcac ctgagaattt gcgccgtcgg caccaatcac
    32581 cagtttcgcg cggatcgttt caccgccctt cagttccagc tcctgaagat cattatctcg
    32641 atgtaatgca atcagcgagg ttggcacacg taacgttact ttcggatgcg cttccagcgc
    32701 ctgccacaac gcctgttgca ggactgtgtt ttccaccatg tagccaagca acggcaactt
    32761 taattcggct gcgtcaaata ccacatgtgc cgtttcccac tcccaggttt ccagtctgcg
    32821 gtaaggatgg caacgcatag cctgtactgc atcccagacc cctaaccctt taagcaatga
    32881 taccgaagcc gcgctgatcg ccgagatccg cacgtccggt tggctgtcag cgacaaacgg
    32941 tgcaggttct gcgtgctcga tcaccgttac cgaaaatccg tgctgtgcca gccccagcgc
    33001 cagtgcgccg ccgaccattc ctccgccgac aatggcaatt tccgttggtt gatttgtcat
    33061 ggtcaatcat cctgttagca atatgcttta agtttacagg atttttaccc ccaagggctg
    33121 ataacgctca tactggtcac aacggcagca aagcattaca ctatgcgccc tgcattcctg
    33181 gctactattt cgcaagagca agtcgatgac caaaaaactc catattaaaa cctggggctg
    33241 tcagatgaac gagtacgatt catcgaagat ggccgatctg ctggatgcca cccacggcta
    33301 tcaactgacc gacgtggcgg aagaagcgga tgtgctactg ctgaacacct gctcaatccg
    33361 cgagaaggct caggaaaaag tcttccatca gttgggtcgc tggaaactgt taaaagagaa
    33421 gaatccagac ctgattatcg gcgtcggtgg ctgcgtggca tcgcaagaag gcgagcacat
    33481 tcgccagcgc gcccactatg tcgatattat ttttgggccg caaacgctgc accgtctgcc
    33541 ggagatgatc aactccgtgc gcggcgaccg cagcccggtt gtagatatca gcttcccgga
    33601 aatcgagaag tttgaccgtc tgccggaacc gcgcgccgaa gggccgaccg cgtttgtctc
    33661 catcatggaa ggctgcaata aatattgcac ctactgcgtg gtgccttaca cccgtggtga
    33721 agaggtaagc cgtccgtctg acgatattct gtttgagatt gcccagcttg cggctcaggg
    33781 cgtgcgtgaa gtcaacctgc tcggtcagaa tgtgaatgcc tggcgtggtg agaactacga
    33841 cggcaccacc ggatcgtttg ccgatctgtt gcgtctggtt gctgcgatcg acgggatcga
    33901 tcgtattcgc tttaccacca gccatccgat cgaattcacc gacgatatca tcgaagtgta
    33961 ccgcgacacg cctgagctgg tgagcttcct gcatctgccg gtacagagcg gttccgatcg
    34021 cattctgaac ctgatgggac gtacccatac ggcgctggag tacaaagcga tcatccgtaa
    34081 actgcgtgcg gcgcgtccgg atattcagat cagttctgac ttcattgttg gcttccctgg
    34141 cgaaaccacc gaagacttcg agaaaacgat gaagctgatt gccgacgtca atttcgacat
    34201 gagctacagc tttatcttct ctgcacgtcc aggaacgcct gccgccgata tggttgatga
    34261 tgttccggaa gaagagaaga agcagcgtct gtatattctg caagaacgta tcaatcagca
    34321 agcgatggca tggagccgtc ggatgctcgg caccacccag cgtattctgg tagaagggac
    34381 ttcgcgtaag agcatcatgg agctttccgg gcgtaccgaa aataaccgcg tggtcaactt
    34441 cgagggcaca ccggatatga tcggtaaatt cgtcgatgta gaaattaccg acgtctaccc
    34501 gaactctctg cgcggtaaag tggtgcgaac tgaagatgaa atgggtctgc gcatggcaga
    34561 aacaccggag tcggtgattg cccgcacccg caaagaaaac gaccttggcg tgggttatta
    34621 tcagccgtaa ttctcaggcc tgccgttccg gtgggccttg ttttttctct ctttatcccc
    34681 atctttatcg caatgcttgc gcctttttcc tgtggcgtga ataatttcct tataggccgg
    34741 tgaatgttca gcttccggcg gcatacagat aagaggaacg gtttgaacat agacactcgc
    34801 gaaatcaccc tggagccagc agacaatgcg cgtctgttga gcctgtgcgg cccgtttgat
    34861 gacaacatca agcagctcga acgccgtctc ggcatcgaga tcaatcgccg cgataaccac
    34921 tttaaactga ccggccgtcc gatttgcgtc accgctgcgg cagacattct gcgtagcctg
    34981 tatgtcgata ctgccccgat gcgcggtcag attcaggata tcgaaccgga acagatccac
    35041 cttgcgatta aagaagcgcg ggtactggag caaagcgcgg agagcgtgcc ggagtacggc
    35101 aaagcggtca atatcaaaac taaacgcggc gtaattaagc cgcgcacgcc aaaccaggcg
    35161 cagtacatcg ccaatattct cgaccatgac attaccttcg gcgttggccc ggcgggtacg
    35221 ggtaaaacct acctggcagt ggctgcggca gttgatgccc tggagcgtca ggaaattcgc
    35281 cgtattctgc tgactcgtcc ggcggtcgaa gccggtgaga aactgggctt cctgcctggc
    35341 gatttaagcc agaaagtaga cccgtatctg cgcccactgt acgacgcgct gtttgaaatg
    35401 ctgggctttg agaaagtcga gaaactgatt gagcgcaacg ttattgaagt cgcgccgctg
    35461 gcctatatgc gtggtcgtac gctgaacgac gcgtttatca ttctcgatga gagccagaac
    35521 actaccatcg aacagatgaa gatgttcctg acccgtatcg gttttaactc aaaagcggtt
    35581 atcaccggcg acgtcacaca gatcgacctg ccgcgtaata ctaaatcagg cttacgtcat
    35641 gccattgaag tgctggccga tgtcgaagag atcagcttta acttcttcca cagcgaagac
    35701 gtggttcgtc acccggtggt ggcgcgtatc gttaacgctt atgaagcctg ggaagaagca
    35761 gaacaaaaac gtaaagcggc gctggcagca gaacgcaagc gcgaagaaca ggaacaaaaa
    35821 tgagtcaggt gatcctcgat ttacaactgg catgtgaaga taattccggg ttaccggaag
    35881 agagccagtt tcaaacatgg ctgaatgcgg tgatcccgca gtttcaggaa gagtcggaag
    35941 tgactattcg cgtggtcgat accgccgaaa gccacagtct gaatctgacc tatcgcggta
    36001 aggataagcc gaccaacgtg ctctccttcc cgtttgaagt gccgcctggc atggagatgt
    36061 cgctactggg cgatctggtt atctgccgtc aggtggttga gaaggaagct caggagcaag
    36121 gcaaaccact ggaggcgcat tgggcgcata tggtggtgca cggcagtctg catttgttag
    36181 gttacgatca cattgaagat gacgaagcag aagaaatgga agccctcgaa acagagatta
    36241 tgcttgctct gggctatgag gatccgtaca ttgccgagaa agaataattt gccaggcatt
    36301 tgactggcag acgtccccgc cgttggtgat taacgacggc gttttgatta actaacatga
    36361 gaacccatac gacgccatga gcgacgacaa ttcacacagt agtgacacga taagcaacaa
    36421 gaagggattt ttctccctgt tactcagcca acttttccac ggtgaaccga aaaaccgtga
    36481 cgaactgctg gcgctgatcc gtgattccgg gcagaacgac cttatcgacg aagatacgcg
    36541 cgatatgctc gaaggggtga tggacatcgc agaccaacgc gttcgcgaca tcatgatccc
    36601 ccgctcccag atgattaccc tgaaacgcaa ccagacgctg gacgaatgcc ttgatgtcat
    36661 catcgagtcc gcccactcac gtttcccggt gattagcgaa gacaaagatc acattgaagg
    36721 gattctgatg gcgaaagact tgctgccgtt tatgcgcagc gatgctgaag ccttcagcat
    36781 ggacaaagtg ttacgtcagg cggttgtcgt tcctgaaagt aagcgcgtag accggatgct
    36841 gaaagagttt cgctctcagc gttaccacat ggcgatcgtt attgacgaat tcggtggggt
    36901 ttccggtctg gtgaccattg aagacatcct ggaactgatt gttggtgaga ttgaagacga
    36961 gtatgacgaa gaagatgata tcgacttccg tcagctgagt cgtcatacct ggaccgtgcg
    37021 cgcactggct tccattgaag acttcaacga agcgttcggc acccacttta gcgatgaaga
    37081 agtcgacact atcggtggtc tggtgatgca ggcgtttggg catcttccgg cgcgtggcga
    37141 aactatcgac atcgacggtt accagttcaa agtggcgatg gccgatagtc ggcgtattat
    37201 tcaggttcat gtcaaaatcc cggatgactc accccagcca aagctggatg aataaaaccg
    37261 aaactggata gataactaca tggcttttgc ctcattaatt gaacgccagc gtattcgcct
    37321 gctgctggcg ttattattcg gtgcctgcgg aacgctggcc ttctctcctt acgacgtctg
    37381 gcctgcggcg attatttcgc tgatggggct tcaggcgttg acctttaacc gtcgcccact
    37441 ccagtctgcc gctattggct tttgctgggg atttggcctc tttggcagcg gtattaactg
    37501 ggtctatgtc agcatcgcga cctttggcgg gatgcctggc ccggttaaca tcttcctggt
    37561 ggtgctgctg gcggcgtatt tgtcgctgta taccggactg tttgctggcg tgctgtcgcg
    37621 tctgtggccg aaaaccacct ggctgcgcgt ggcgattgcc acccctgctc tctggcaagt
    37681 gaccgagttt ctgcgcggtt gggtgctgac tggcttcccg tggttacagt tcggctatag
    37741 ccagattgat ggcccgttaa aagggctggc accgctaatg ggcgtggaag ccattaactt
    37801 cctgctgatg atggttagtg gcctgctggc actggcgttg gtcaaacgca actggcgtcc
    37861 gctggtggtg gccgtcgtgc tgtttgccct acccttcccg ctgcgttaca tccaatggtt
    37921 taccccgcaa ccggagaaaa ccattcaggt ttcgatggtt cagggcgata ttccgcaatc
    37981 gctgaaatgg gacgaaggcc agcttcttaa tacgctaaag atttactaca acgcaacggc
    38041 accgctgatg ggcaaatcat cgttgattat ctggccggag tcggcgataa ccgatctgga
    38101 aattaatcag caaccgttcc tcaaagcact ggacggtgag ttgcgtgata aaggtagctc
    38161 gctggtaacc gggattgtcg acgcgcgtct caataagcag aaccgctacg atacctacaa
    38221 caccatcatc acgctgggta aaggtgcacc gtacagctac gaatcagccg atcgctataa
    38281 caaaaaccat ctggtgccgt ttggcgagtt tgtcccgctg gagtcgattc tgcgtccgtt
    38341 agcaccgttc tttgatctgc cgatgtcgtc gttcagccgt gggccatata tccagccgcc
    38401 gctgtcggca aatggtattg agcttactgc ggctatttgc tacgagatca ttctcgccga
    38461 gcaagtgcgc gataacttcc gcccggatac cgactacctg ctgactatct ccaacgatgc
    38521 gtggtttggt aaatctattg gtccatggca acacttccag atggcgcgaa tgcgtgcgct
    38581 ggagctggcg cgcccactgt tgcgcagcac caacaacggc attacggcgg tgattggccc
    38641 gcagggtgag attcaggcga tgatcccgca gttcgcccgc gaggtgttaa ccactaacgt
    38701 gacgccgacc accggactca caccatacgc acgtaccggc aactggccgc tgtgggtgct
    38761 gacggcactg tttggttttg ctgctgtgtt gatgagtctg cgtcagcgac gtaaataatc
    38821 ccttcttatc gtgccggaat acatcccata tttcggcacg ctcaccggat gcggcatcag
    38881 tgcattatcc ggataacaat attctcctat ccaactttgg cacatctatt gctttgttat
    38941 acaaggcaaa gcttgaacca gcatcagtac aacccggttg caccacagca atccaccggt
    39001 agcaccaaaa gagtgcattt taagaatttt gcttcagaat ggtgcggtgc acctcgcaaa
    39061 aacaaacaat aaaacatcaa tatctttaca tttagccaaa ctaaatgtta cctaacaggc
    39121 acaacactgc acaataaagt tgcagacgat aacaacacaa acactcacaa cgggtatcca
    39181 tgcgttctta acgcagaaga taaaggtgtt ggatatgcaa ttacgtaaac ctgccacagc
    39241 aatcctcgcc ctggcgcttt ccgcaggact ggcacaggca gatgacgccg ccccggcagc
    39301 aggcagtacg ctggacaaaa tcgccaaaaa cggtgtgatt gtcgtcggtc accgtgaatc
    39361 ttcagtacct ttctcttatt acgacaatca gcaaaaagtg gtgggttact cgcaggatta
    39421 ctccaacgcc attgttgaag cagtgaaaaa gaaactcaac aaaccggact tgcaggtaaa
    39481 actgattccg attacctcac aaaaccgtat tccactgctg caaaacggca ctttcgattt
    39541 tgaatgtggt tctaccacca acaacgtcga acgccaaaaa caggcggctt tctctgacac
    39601 tattttcgtg gtcggtacgc gcctgttgac caaaaagggc ggcgatatca aagattttgc
    39661 cgacctgaaa ggcaaagccg tagtcgtcac ttccggcact acctctgaag ttttgctcaa
    39721 caaactgaat gaagagcaaa aaatgaatat gcgcatcatc agcgccaaag atcacggtga
    39781 ctctttccgc accctggaaa gcggtcgtgc cgttgccttt atgatggatg acgctctgct
    39841 ggccggtgaa cgtgcgaaag cgaagaaacc agacaactgg gatatcgtcg gcaagccgca
    39901 gtctcaggaa gcctacggtt gcatgttgcg taaagacgat ccgcagttca aaaagctgat
    39961 ggatgacact atcgctcagg tgcagacctc cggtgaagcg gaaaaatggt ttgataaatg
    40021 gttcaaaaat ccaattccgc cgaaaaacct gaacatgaat ttcgaactgt cagacgaaat
    40081 gaaagcactg ttcaaagaac cgaatgacaa ggcactgaac taattacaag aaccaggggc
    40141 ggaaattcca gccctctcga ttgttacgta gcacggacag actatacgcc tgatggtcgt
    40201 tccccatcgg gcctgaaaac cgcaatacgc tgggtaacaa tcttcgaggg tagcagttaa
    40261 cgctgctacc ctcttttttc tggagtagat ttatgtctat agactggaac tggggtattt
    40321 ttttacaaca agccccgttc ggcaatacca cctatcttgg ttgggtctgg agtggttttc
    40381 aggtgacgat cgctttgtcg atctgcgcct ggattatcgc tttcctcgtc ggttcatttt
    40441 tcggcatttt acgtaccgtt ccaaaccgtt ttctctccgg tctgggtacg ttgtatgtcg
    40501 aactgttccg caacgtgccg ttgattgtgc aattctttac ctggtatctg gtgatcccgg
    40561 agctgctgcc ggagaaaatc ggcatgtggt ttaaggccga gctggatccc aatattcagt
    40621 ttttcctttc atccatgctc tgcctggggc tgtttaccgc cgcccgtgtt tgcgaacagg
    40681 tacgcgccgc tattcagtcg ctaccgcgtg ggcagaagaa tgccgcgctg gcgatgggcc
    40741 tgacgctacc gcagacttat cgatacgtac tgctgcctaa tgcttatcgc gttatcgtcc
    40801 cgccgatgac ttccgagatg atgaacctgg tgaaaaactc cgccatcgcc tcgaccatcg
    40861 gtctggtgga tatggcggcg caggcgggta aattgctcga ctactcggct cacgcctggg
    40921 agtcgtttac cgccatcacg cttgcgtatg tgttgattaa cgcttttatc atgctggtga
    40981 tgacgctggt tgaacgtaaa gttcgcctgc ccggcaacat ggggggcaaa taatgtacga
    41041 gtttgactgg agttccattg ttccttccct gccatatctg ctcgacgggc tggtgatcac
    41101 cctgaaaatc accgtcacgg cagtcgtgat tggtattttg tggggtacca tgctggcggt
    41161 gatgcgcttg tccagcttcg cgcctgtttc gtggtttgcc aaagcctacg ttaacgtatt
    41221 ccgctcgatt cctttagtca tggttttgct gtggttttac ttgattgtgc cgggatttct
    41281 gcaaaatgtg ctgggattat cgccaaaaaa tgatattcgt ttaatttcgg cgatggtggc
    41341 gttttcgatg tttgaagcgg cctactattc agagattatc cgtgccggta ttcaaagtat
    41401 ttcccgtggt cagtcgagcg ccgcgctggc gttggggatg actcactggc agtcgatgaa
    41461 actgattatt ctgccgcagg ctttccgcgc gatggtgccg ctgctgctca ctcagggcat
    41521 cgtactgttc caggatacct cgctggtgta tgtgttatct ctggccgatt tcttccgtac
    41581 cgcctcaacc attggtgagc gtgatggtac gcaggtcgag atgatcctgt ttgccggatt
    41641 tgtttatttc gttattagcc ttagcgcgtc gttgttggtc agctacttga aaagaaggac
    41701 agcataatga ttaccctgaa aaatgtttca aaatggtatg gtcactttca ggtgctgacc
    41761 gactgctcaa ccgaagtgaa aaaaggcgaa gtggtggtgg tttgcggccc gtctggttcc
    41821 ggcaaatcaa cgctgattaa aaccgtcaac ggcctcgaac cagtgcagca aggtgaaatc
    41881 accgtcgatg gtatcgtggt taacgacaag aaaaccgatc tggcaaagct gcgctcccgc
    41941 gtcgggatgg tattccagca tttcgagctg ttccctcatc tgtcgattat cgaaaacctg
    42001 accctggcgc aggtgaaagt gcttaaacgc gataaagcgc ccgctcgtga aaaagcattg
    42061 aaattgctgg agcgcgtggg actttccgca catgccaata aatttccggc gcagctttcc
    42121 ggcggtcagc agcagcgcgt ggcaatcgct cgcgcattgt gtatggctcc tattgcgatg
    42181 ctgttcgacg aaccgacatc ggcgctggat ccggagatga tcaacgaagt gctggacgtg
    42241 atggtggaac tggcgaacga aggaatgacc atgatggtgg tgacccacga aatgggcttt
    42301 gcccgcaaag tggcgaatcg ggtgatcttt atggacgagg gtaaaattgt cgaagactcg
    42361 ccgaaagacg ctttcttcga tgatccgaaa tcggaccgcg caaaagactt cctcgcgaaa
    42421 atcctgcatt aatcactctg gcgcacatac ccaattgcgc gccatctcac gctttatcgt
    42481 ttcgcccctc gtatctgtta catcctggcg ttacccttgt cgcaaagaag cacacaacaa
    42541 ggagcaacaa tggcactgcc aattctgtta gattgcgacc caggtcatga tgacgctatc
    42601 gcaatagttc tcgcactcgc ctcaccagag cttgatgtca aagcaattac gtcttccgcc
    42661 ggaaaccaga caccagaaaa aaccttacgc aatgttctgc gtatgctgac cttgcttaat
    42721 cgcaccgata ttccggtggc aggcggcgcg gtaaaaccgt taatgcgtga gttgattatc
    42781 gcggacaatg tgcacggcga aagcggtctc gacggcccgg cattaccgga accgacgttc
    42841 gcaccgcaaa actgtacggc ggtggagctg atggcgaaaa cgctgcgcga aagtgcggaa
    42901 cctgtcacga ttgtttctac cggaccgcaa actaacgttg ccttgctgct caatagccat
    42961 ccggaactgc atagcaaaat tgcccgtatc gtgattatgg gcggcgcaat ggggcttggt
    43021 aactggacgc ctgcggctga atttaatatt tacgttgacc cggaagcggc agaaattgtc
    43081 ttccagtcag ggatcccggt ggtgatggcc ggtctggatg ttactcataa agcgcaaatc
    43141 cacgttgaag acaccgagcg tttccgcgcg attggcaacc ctgtttcaac cattgttgcc
    43201 gaactgctgg atttcttcct cgaatatcat aaagacgaaa aatggggctt tgtcggcgca
    43261 ccgctgcatg acccatgcac catcgcctgg ctgttgaaac ccgagttgtt taccactgtt
    43321 gagcgctggg ttggcgtgga aacacagggg aaatataccc agggtatgac ggttgttgat
    43381 tattattatc tgactggcaa taaaccgaat gccactgtaa ttgtcgatgt tgatcgtcag
    43441 ggctttgttg atttactggc tgatcgtctg aaattttacg cttaacactc gtcattgatt
    43501 aagatcacca cgcgttacct gggtggtctt aatctattta tctctttttc tttcaagata
    43561 taaatgcaat ggataatgca gaactcgcca taggtatcga tctcggcact accaatagtt
    43621 taattgccgt ctggaaagac ggtgccgcgc aattaattcc aaataagttc ggtgaatatt
    43681 taacaccatc cataattagc atggatgaaa ataatcatat tttagtcgga aaaccggctg
    43741 tatcacggcg tacttcgcat ccggataaaa cggcagcgtt atttaaacgt gcaatgggca
    43801 gctggcggtt aggcagcgac acatttaacg cgccagaact gtcctctttg gtattacgct
    43861 cattaaaaga agatgccgaa gaatttctgc aacgtccgat taaagatgtg gtgatctccg
    43921 ttccggctta tttcagtgat gaacaacgca agcatacccg tttagcagcg gacttagccg
    43981 ggttaaatgc ggtacgctta attaatgaac ccacagcagc tgcgatggcg tatggcctgc
    44041 atacccaaca aaatacccgt tcgctggtgt ttgatctcgg tggcggcacg tttgacgtta
    44101 cggtgcttga gtacgccacg ccggtgattg aagttcacgc ctccgctggc gacaacttcc
    44161 tcggtggcga agattttacc catatgttgg tcgatgacgt tttaaagcgc gcggatgtca
    44221 caaggaccac gcttaacgag agtgaactgg cagccttgta cgcctgtgtg gaagcggcaa
    44281 aatgtagcaa tcaatcgcca atgcacattc gctggtagta ccaggatgaa acgcgggaat
    44341 gcgaatttta cgagaacgaa ctggaagatt tgtggttgcc gctgctcaat cgcttgcgag
    44401 tgccgattga acaggcgttg cgcgatgcgc gtctgaagcc gagtcaaatc gacagtctgg
    44461 tgctggttgg cggcgcgtca caaatgccgc tggtgcagcg aatcgccgtg cgtctgtttg
    44521 gcaaattacc gtatcaaagt tacgatccga gcaccattgt cgcgctgggc gcagcaatcc
    44581 aggccgcctg ccgcttacgc agtgaagata ttgaagaagt gatcctcact gatatttgcc
    44641 cttactcgtt gggcgttgag gttaaccgcc agggcgtttc cggcattttc tcgccgatta
    44701 ttgaacgaaa caccactgtg cccgtgtcgc gtgtagaaac ttattcaacc atgcacccgg
    44761 aacaggattc aattacggtt aacgtctatc agggagaaaa ccacaaagtt aaaaacaaca
    44821 ttctggtgga atccttcgat gtgccgttga agaaaaccgg ggcttatcag tcgattgata
    44881 ttcgctttag ttatgatatc aacgggttgc ttgaagttga cgtgcttctg gaagacggca
    44941 gcgttaagtc cagagtgatt aaccacagcc cggtaacatt gagcgcgcag cagattgaag
    45001 agagtcggac gcggttatcc gcattgaaaa tttatccgcg cgatatgctc atcaatcgca
    45061 cctttaaagc caaactggaa gagttgtggg cgcgggcgct gggtgacgag cgagaagaga
    45121 ttggccgggt gatcaccgat tttgatgcgg cgctgcagtc aaacgatatg gcccgcgtcg
    45181 atgaggtcag gcggcgggcg agcgtttatt tagccattga gacctcataa tcacggctgc
    45241 cggatgcggc gtaaatgctg catccggcat cggcgcaaaa aacacatcgt caactaaaca
    45301 acatcgcgat tttgccgaga gcataaccta taaccagcac cgttaaactg attttgataa
    45361 tgctggtaat gctctgtggt ttttcaccct catcattttt ttcttcatgg ttttcaggtg
    45421 ttggctcatc gacatcttca tcctccggct cgagttttac tttcgtcaac agcatccggg
    45481 tgataatggc ctctaatgga tgagcatctc cgccttccag ccttgtatta agacgggcca
    45541 gcatctcttg tttcggaaac gcttcaagta ctacctgttc cgctacccga tacaaatcca
    45601 gtaacggtga atcttcttct gtctcgctac cgactctcga tttccaccac gcccatgctt
    45661 tgcgtttgaa gaataacgac tcaataaacg attgacctaa cggcgataat tttccgctat
    45721 gtaaaatttc cgcccataac agccgcgtcc gggcgctctc accaggaaca tatttcagcg
    45781 gttgaccttg cgcatctacc atgctccgcg cttcgataac catcactaat aacgcgaaat
    45841 aatccgggca atgacgcttg ctccactgca acaacaaatt atcagcttgt gtagatgggt
    45901 aactttccca gtacgcgccg agatcagcaa gcaggctttc cccctcgccg cagtacaaac
    45961 gttgtgcaca ctggtaatac cgggcatctt cgctttctgg ttcttccgct tgccactgtt
    46021 tggctacagg aatgagttcc accatacctg tttgcaccga gctgtaccag cgcaacagtt
    46081 tgcgatgtaa ctttgcatca tcaggaatga accacggtcc ttccatcgcc aaaaacgcag
    46141 tgacatagtc aggatggtaa cggcagcaac gttccagcgc gaagtaatat tcaacagtct
    46201 ggttctgtaa cgcgacggga tattccagaa ggctaaggaa atcgaaacaa tcaccagcct
    46261 gaatggcctc aagaaaagcc tccctttctt cctcatccat tccttcgctg tcattaaacg
    46321 aatgccagtt caaacgctct gccaacaaag aagcacaatt taatgacaga tatcgcgctt
    46381 ctatagcgat tgcacacagc ggccagcgta attgatcgac atcctccatg ttccaggtat
    46441 ttaattgctg aataaatttc tgccaggcgg agggctgaaa acgatcactt tctgaatcca
    46501 gtaatgtcct gaatgcacgt agtatttcat gttcagcggt ggcatctttt tcttcatcat
    46561 ctgcttcctc gacaggattt accgccagtc gcagcgcctc ttcataggcc tggcgtaatg
    46621 ctttaaaccc ctgcggatcg gtttcgggat gacacaacgg taagcgagca agataagcct
    46681 ggcggataat gtctatttgc gtcgtgcttt caatttcgag gatttgccaa catgttttca
    46741 taggtcgctc acatccttaa gcgttaccgg gcattcaggc tggacagtta atggctgcca
    46801 gggaagcgtg gcatagaaac ggtcgcttcc cgctgccagc tcttcgaaca tccgactccc
    46861 ggcgctatcg tatttgtaca gggttagcgc ctcgcgtagc gaagcgttgt cttcgcactg
    46921 agaagattgc cagtagagac gccctagtga ataggcagaa aaatagtgca tccagctgtg
    46981 atagtaatgg tgagctcgtg catagacgcg cgattgcagc cataaactct cttcttccgt
    47041 cagccagttg ttgagcacac cggcccgtga cagaaacccc attcgaacat aatcccaggc
    47101 tttgattccc ccttctccac agcacatagc ggtttgggca acgaattggg tataaatcaa
    47161 ccctctctcc gagccaccag caattaatgc tttccactct tcaggggaag agcgttgcca
    47221 tgtcagatat aagccagcta aatcattggc atgaccatca tcagtcatct gataaatagt
    47281 ctgaattaaa tcatcacgat tatttattcc ccaggatgat gagagatcga attcttttat
    47341 cccggtatac atttttggcg agtcatgcgc gggtttgcaa ctctgattaa gtatttccat
    47401 gggcgcagaa agcgcaaaaa gaagatattg ttcttcctta ttcatttttt aataatccct
    47461 taagtgcatt aactgactac ttttttatta ttgatgagta taaacttgac gctttcaaaa
    47521 taaaaagaaa atcgaagcat tcacacatga ataaaaaatt aatgtatata ttcgcaattt
    47581 ttatagttgc agcaattacc tgtattagcc aacccaagaa aacgatgttg cgtgataaag
    47641 ccatggtgaa ttatgccttt gattatttaa gatcaccagg cagtctaccg ttaaccacgg
    47701 cagccacgga gctttccgcg attcatggtc actcaacatc gcaatatcgc cttggcgaat
    47761 tttatcttca tggtagcgac ggtaaaccac tggattatac acaggcgaga tactggtatg
    47821 agcaatcagc agaacaggaa aatccgcgcg cacaaagcaa actgaggtgg atctacctga
    47881 aaggcctggg agtcaaaccc gatacccgta aagcaattct ctggtataag gaagcagctg
    47941 aacaagggta tgctcatgct caatatactt tgggtttgat ctacagaaat ggcactggta
    48001 ttaatgttaa ccattatgaa tctcaaaaat ggttaaaact ggccgccaaa caacattaca
    48061 aaaatgcgga aagattactt gccggacttc ccgcacatta acgtcgactg tcacccgaag
    48121 agatggagta ttttccctat caaaccggca aagataaaaa tgtagaaaat aattttgatg
    48181 acgcccagac agcccagccc gccatcatca acgggcattg gctgtggttg cggctccggc
    48241 tcgacggttt cctgaccggt aaaattcgcc gcctgaatta ataatcgacg aataattaat
    48301 gcttccagtg gacaacgatc ttctgcatca aggcggctca caatcggcat taatgattct
    48361 tctgtcggtt ggctggcctg cactaattgt ttggcaacta aatataatgg ccataaggga
    48421 tgttctgtat cacgcgtgag tgagatccgc ggcttacgtc cttcgtgcaa aaccatctcg
    48481 accagtgaac gccccagcgc agagagtttt tcatcgctga gtatttcgta taaatgaaaa
    48541 cgcgtacggg cgctgtcgcc ggggacataa agtagcggtt ttccctgatc gttaaccaga
    48601 tcacgcgctt caatcatcat caccactaat gggtaataat agacccgatg ctgacggcac
    48661 cattgcaaca ttaaagcatc cgcctgggtg gagggatact cgcgccagta gtaacaaagt
    48721 tctgccagca ggctttcgcc ttctccacaa tagacgcgtt gtgcgtattc ataataacgc
    48781 gcatcttcat tgtcaggttc agcttcccgc cacgaccggg cgtaatccag gagttcgggg
    48841 attcccaggc ggcttgcgct ataccagcgg agtaaatttc tgtgcagttt tgcatcatcg
    48901 gggataatca ctgcgcgatg ttgcgctaaa tacaatgtca gccattctgg atgcgatgac
    48961 caaatccgag cgaacatttg gtaaaaatcg atggtgtcat tttgcacggc aactggcaga
    49021 tgcagaatgg tctggaagtt aaaaacattc cccttcgcaa tggcatataa aaaggattcc
    49081 agttcttctt cgtctatttc cccgacgttt tcctcctgca accaccgcaa tctttctgct
    49141 aataacacca cgcactcgaa ggataaatgg gcagtgttca tggcgattgt gcacagcgac
    49201 cagcgtaatt catcaatctc ttccatcgag caataattta attgctgaat gaatcgctgc
    49261 caggcggagg acagaaaacg ttcgctatca gaggcaagta acgcgcgaaa ggcgagcaga
    49321 atttcatgtt ctgctacctc atattcttct ggttgccaaa cagatttagc aggcgactgc
    49381 gcaatccgta gcgcctcctc atacgcctga cgaagttgtt taaaaccctg cggatcggtt
    49441 tctggatgaa aggacggtaa cagcgccaga taagcgcggc ggataatatc gacgtcagtc
    49501 gtttcctcta tatcgaggat cttccagcaa tttttcataa gtcgctcata tccttaagcg
    49561 tttccgggca tgtggggtag tcagccagtg gttgccagga caaggtaggg taatacgcat
    49621 catcactggc aaataattga taaaacatat cattgccgga atcgtcgtat tctttacgcg
    49681 ctaagaattc gcgtaataaa ggcagatgat catcttcggg agactgccaa tattgccgac
    49741 caaaggtata accggcaaag tattgccgcc agttactata ataacgtaga gcccgtagat
    49801 gaatacgcga ctggatccat agactttctt cttcactcaa ccagttatta agtacgccca
    49861 ttcgacttaa aaaacccatc cggacataat cccatgcttt aattcctccc tcaccacagc
    49921 attcggctgt gctggcgaca aattgcgcat aagcctgacc ttgctcattt aattcagcaa
    49981 gatagtcacg ccattcacat ggagaataac gaaaccagcg gtgataaaac cctgccaacc
    50041 gagcggcatg accgttctcg atcattcggt gaattatgcg cagcaggtca tcacggtcat
    50101 caattcccca tgaggaagaa aggtcaaaaa agcatgtacc taaatacatt ttgggggatg
    50161 aatgcgaagg taaacagcat tcattgcgta tttccatcgg agtggaaagt gcaaacaata
    50221 ttttttgcga ccccatgtcc atataattta tccttaaaca ttcatttaat gaatgtaaac
    50281 tacatttcga ggctcagtta tttatacttt tctgacgcaa cataatacgc ataaaataag
    50341 gcttacgcaa aaatggaata cataaaaata taatatattt tatctacaac cataaggaaa
    50401 tgataatgat ttttacgtca agttgctgcg ataatttatc aatagatgag attatcgaac
    50461 gtgctgaaaa aggcgattgc gaggctcaat atattgttgg gttttattat aatcgcgata
    50521 gcgcaattga ctctccggac gacgaaaagg ccttttactg gctgaagctg gccgctgagc
    50581 aaggtcattg tgaagcacag tattccttag ggcaaaagta taccgaggat aaaagccgtc
    50641 ataaagataa tgagcaagcc atcttctggc tgaaaaaagc tgccctacaa ggccatactt
    50701 tcgcttccaa cgcccttggc tggatactgg atcgtggaga agaccccaac tataaagaag
    50761 cggttacctg gtatcagata gccgcggaga gcggaatgtc ttatgcgcaa aataatcttg
    50821 gatggatgta cagaaatggc aacggagtcg cacaagacta tacgctggca tttttctggt
    50881 acaaacaagc tgcattacaa ggccatagtt acgcacaaga caatctggcc gatctttata
    50941 aagacggaga aggcgttgct caaaacaaga cactcgccgc attctggtat ttgaaaagcg
    51001 cacagcaggg taatcggcac gcccagtttc aaattgcatg ggattataac gctggcgaag
    51061 gggtggacca ggactataag caagcgatgt actggtatct gaaggctgcc gctcaggaaa
    51121 gcgtcggcgc ttacgtcaac atcggttata tgtataaaca cggacaaggc gttgagaaag
    51181 attatcaggc tgcctttgaa tggtttacga aagccgctga atgcaatgac gccactgcct
    51241 ggtataacct ggccattatg tatcactacg gagaaggaag acctgtcgat ctccgacagg
    51301 ctctcgacct gtatcgtaaa gttcagtcat ccggaaccag agatgtcagt caggaaattc
    51361 gtgagactga agctttactg tagaaataaa aacaagggcc ggggaatttc attctgccca
    51421 tattctcttt attgctgtgc gaaatcagac tattacggct caaacgggcg tctctggaac
    51481 tggtcataac cacatttcgg gcatagcgtc agcacttccg gtgtgtagat cgggagatgg
    51541 aagtgacatt tctcgcagac cagatttccc agcccgacca cttctccgct gtgataaacc
    51601 ccatgatgat tgaggtcctg gaaaatttcg cgccattcaa gctgcgtttt atcggtgatg
    51661 tctgccagct cctgccacaa gctttcttta atcacccgca taaagacgct gtcagattct
    51721 tctttcaggc tctcttcata gctcatggcg aactcttcca ggtcacgtct gacagctcgc
    51781 gtcagctcat cgatctcggt tcgcgttaac tcccctgttt ttattacgcg ctcgcgcgcc
    51841 tgttccacca gtgcgtcgat atcacgttcg ccattgcgca ggcgttcgtt cagtgacgcg
    51901 accagttcac ggtaatattg agcaaccttg ttcatcgttt catctcccgg gtcgttaact
    51961 gactataata atagacgttc tttgttcctt gctttgctaa tacgcccaca aactctgtaa
    52021 attaatgtac gaataatccg ggcatcattt caacgtcgag tgcgcgaaag gctgttttga
    52081 cgcggacgtt tgggctatgc tatgcggatc tgaaaaacca cctcaacgct acatttgtag
    52141 ccgtattgaa aacaggacca ctggctgcca tgcaagagca ataccgcccg gaagagatag
    52201 aatccaaagt acagcttcat tgggatgaga agcgcacatt tgaagtaacc gaagacgaga
    52261 gcaaagagaa gtattactgc ctgtctatgc ttccctatcc ttctggtcga ctacacatgg
    52321 gccacgtacg taactacacc atcggtgacg tgatcgcccg ttaccagcgc atgctgggca
    52381 aaaacgttct gcagccgatc ggctgggacg cgtttggtct gcctgcggaa ggcgcggcgg
    52441 tgaaaaacaa caccgctccg gcaccgtgga cgtacgacaa catcgcgtat atgaaaaacc
    52501 agctcaaaat gctgggcttt ggttatgact ggagccgcga gctggcaacc tgtacgccgg
    52561 aatactaccg ttgggaacag aaattcttca ccgagctgta taaaaaaggc ctggtatata
    52621 aaaagacttc tgcggtcaac tggtgcccga acgaccagac cgtactggcg aacgaacaag
    52681 ttatcgacgg ctgctgctgg cgctgcgata ccaaagttga acgtaaagag atcccgcagt
    52741 ggtttatcaa aatcactgct tacgctgacg agctgctcaa cgatctggat aaactggatc
    52801 actggccaga caccgttaaa accatgcagc gtaactggat cggtcgttcc gaaggcgtgg
    52861 agatcacctt caacgttaac gactatgaca acacgctgac cgtttacact acccgcccgg
    52921 acacctttat gggttgtacc tacctggcgg tagctgcggg tcatccgctg gcgcagaaag
    52981 cggcggaaaa taatcctgaa ctggcggcct ttattgacga atgccgtaac accaaagttg
    53041 ccgaagctga aatggcgacg atggagaaaa aaggcgtcga tactggcttt aaagctgttc
    53101 acccattaac gggcgaagaa attcccgttt gggcagcaaa cttcgtattg atggagtacg
    53161 gcacgggcgc agttatggcg gttccgggtc acgaccagcg cgactacgag tttgcctcta
    53221 aatacggcct gaacatcaaa ccggttatcc tggcggctga cggctctgag ccagatcttt
    53281 ctcagcaagc cctgactgaa aaaggcgtgc tgttcaactc tggcgagttc aacggtcttg
    53341 accatgaagc ggccttcaac gccatcgccg ataaactgac tgcgatgggc gttggcgagc
    53401 gtaaagtgaa ctaccgcctg cgcgactggg gtgtttctcg ccagcgttac tggggcgcgc
    53461 cgattccgat ggtgacgctg gaagacggta ccgtaatgcc aaccccggac gaccagctgc
    53521 cggtgatcct gccggaagat gtggtgatgg acggcattac cagcccgatt aaagcagatc
    53581 cggagtgggc aaaaactacc gttaacggta tgccagcgct gcgtgaaacc gacactttcg
    53641 acacctttat ggagtcctcc tggtactatg cgcgctacac ttgcccagag tacaaagaag
    53701 gtatgctgga ttccgaagcg gctaactact ggctgccggt ggatatctac attggtggta
    53761 tcgaacacgc cattatgcac ctgctctact tccgcttctt ccacaaactg atgcgtgatg
    53821 caggcatggt gaactctgac gaaccagcga aacagttgct gtgtcagggt atggtgctgg
    53881 cagatgcctt ctactatgtt ggcgaaaacg gcgaacgtaa ctgggtttcc ccggttgatg
    53941 ctatcgttga acgtgacgag aaaggccgta tcgtgaaagc gaaagatgcg gcaggccatg
    54001 aactggttta taccggcatg agcaaaatgt ccaagtcgaa gaacaacggt atcgacccgc
    54061 aggtgatggt tgaacgttac ggcgcggaca ccgttcgtct gtttatgatg tttgcttctc
    54121 cggctgatat gactctcgaa tggcaggaat ccggtgtgga aggggctaac cgcttcctga
    54181 aacgtgtctg gaaactggtg tacgagcaca cagcaaaagg tgatgttgcg gcactgaacg
    54241 ttgatgcgct gactgaagat cagaaagcgc tgcgtcgcga tgtgcataaa actatcgcta
    54301 aagtgaccga tgatatcggc cgtcgtcaga ccttcaacac cgcaattgcg gcgattatgg
    54361 agctgatgaa caaactggcg aaagcaccga ccgatggcga gcaggatcgc gctctgatgc
    54421 aggaagcact gcaggccgtt gtccgtatgc tcaacccgtt caccccgcac atctgcttca
    54481 cgctgtggca ggaactcaaa ggcgaaggcg atatcgacaa cgcgccgtgg ccggttgctg
    54541 acgaaaaagc gatggtggaa gactccacgc tggtcgtggt gcaggttaac ggtaaagtcc
    54601 gtgccaaaat caccgttccg gtggacgcaa cggaagaaca ggttcgcgaa cgtgctggcc
    54661 aggaacatct ggtagcaaaa tatcttgatg gcgttactgt acgtaaagtg atttacgtac
    54721 caggtaaact cctcaatctg gtcgttggct aagcgcggga ggaagcgtgc gatatctggc
    54781 aacactgttg ttatctctgg cggtgttaat caccgccggg tgtggctggc atctgcgtga
    54841 caccacgcag gttccttcca ctatgaaggt catgatcctg gactcaggcg atccgaacgg
    54901 gccattaagc cgtgcggtgc gtaaccagtt acgtctgaat ggtgtcgagt tgcttgataa
    54961 agaaaccacg cgtaaggacg ttccatccct gcgtttgggt aaagtgagca tcgcgaaaga
    55021 taccgcatcg gtattccgta acggtcaaac agcagagtat cagatgatca tgacggtgaa
    55081 tgcgaccgtg ttgatccccg gccgtgatat ctacccgatt agcgccaaag tcttccgttc
    55141 attcttcgat aacccgcaaa tggcgttagc gaaagataac gaacaagaca tgatcgtaaa
    55201 agagatgtac gaccgtgctg ccgaacagct gattcgtaag ctgccaagca tccgtgctgc
    55261 ggatattcgt tccgacgaag aacagacgtc gaccacaacg gatactccgg caacgcctgc
    55321 acgcgtctcc accatgctgg gtaactgatg attcggttgt acccggaaca actccgcgcg
    55381 cagctcaatg aagggctgcg cgcggcgtat cttttacttg gtaacgatcc tctgttattg
    55441 caggaaagcc aggacgctgt tcgccaggta gctgcggcac aaggattcga agaacaccac
    55501 actttttcca ttgatcccaa cactgactgg aatgcgatct tttcgttatg ccaggctatg
    55561 agtctgtttg ccagtcgaca aacgctattg ctgttgttac cagaaaacgg accgaatgcg
    55621 gcgatcaatg aacaacttct cacactcacc ggacttctgc atgacgacct gctattgatc
    55681 gtccgcggta ataaattaag caaagcgcaa gaaaatgccg cctggtttac tgctcttgcg
    55741 aatcgcagcg tgcaggtgac ctgtcagaca ccggagcagg ctcagcttcc ccgctgggtt
    55801 gctgtgcgcg caaaacagct caacttagaa ctggatgacg cggcgaatca ggtgctctgc
    55861 tactgttatg aaggtaacct gctggcgctg gctcaggcac tggagcgttt atcgctgctc
    55921 tggccagacg gcaaactgac attaccgcgc gttgaacagg cggtgaatga tgccgcgcat
    55981 ttcacccctt ttcattgggt tgatgctttg ttgatgggaa aaagtaagcg cgcgttgcat
    56041 attcttcagc aactgcgtct ggaaggcagc gagccggtta ttttgttgcg cacattacaa
    56101 cgtgaactgt tgttactggt taacctgaaa cgccagtctg cccatacgcc actgcgtgcg
    56161 ttgtttgata agcatcgggt atggcagaac cgccggggca tgatgggcga ggcgttaaat
    56221 cgcttaagtc agccgcagtt acgtcaggcc gtgcaactcc tgacacgaac ggaactcacc
    56281 ctcaaacaag attacggtca gtcagtgtgg gcagagctgg aagggttatc tcttctgttg
    56341 tgccataaac ccctggcgga cgtatttatc gacggttgat atgaaatctt tacaggctct
    56401 gtttggcggc acctttgatc cggtgcacta tggtcatcta aaacccgtgg aaacgctggc
    56461 gaatttgatt ggtctgacgc gggtcacaat catccctaat aatgttcctc cgcatcgtcc
    56521 ccagccggaa gcgaacagcg tgcagcgtaa acacatgctt gaactggcga ttgccgacaa
    56581 gccattattt actcttgatg aacgcgagct aaagcgcaat gccccctctt acactgcgca
    56641 aacactgaaa gagtggcggc aggaacaagg accggacgtg ccgctggcgt ttattattgg
    56701 tcaggattca ttgctgacct ttccgacctg gtacgaatac gaaacgatac tcgacaatgc
    56761 acatttgatc gtctgtcggc gtccaggtta cccacttgaa atggcgcaac cgcaatacca
    56821 gcaatggctg gaagatcatt tgacacataa cccggaagat cttcaccttc agcctgccgg
    56881 taaaatttat ctggctgaaa cgccgtggtt taacatctcg gcgaccatca tccgcgaacg
    56941 tttgcaaaac ggtgaatcgt gtgaggattt attgccggaa ccggtattga cttacattaa
    57001 ccaacaaggc ttgtatcgct gatacctgtc gttacggagg gcatgatgcg actgtggtta
    57061 attcgtcatg gcgaaacaca agcgaatgtc gatggtctgt acagcggtca tgcgcccaca
    57121 cccctgaccg cacgcggtat tgagcaagcg caaaatctgc atacactgct agatgatgtt
    57181 tcctttgacc tggttttatg cagtgaactg gaacgggcac agcataccgc gcgactggtt
    57241 ctcagtgacc gccagctccc cgttcatatc atccctgaac tcaacgaaat gttttttggc
    57301 gactgggaga tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg
    57361 tggtgcaatg actggcagca tgctatcccc acgaatggtg aaggatttca ggcattttcg
    57421 caacgtgtgg aacgctttat cgcaaggctt agtgaatatc agcactatca gaatatttta
    57481 atcgtcagcc atcagggcgt actgagtctg ttaattgctc gtttaattgg catgcctgcc
    57541 gagtctatgt ggcattttcg cgttgaccag gggtgttgga gcgccattga tatcaaccaa
    57601 aaattcgcga cgctacgcgt cctcaatagc cgtgccatcg gggtcgagaa cgcatgactt
    57661 ttctgttttt ttacgggtaa gccgcaacga ccattgacag ccccgggcag gctgatattc
    57721 tccgcagcca gactttttcc gccagacacg actttgtaga aattgtttta caaaaatggc
    57781 gatgcaatct gcggcgcggg gtgggatgat agcccacttt cgaaagccga ttcggcgaca
    57841 attgtcccga aatcgcctct ggttcaggta tactgacaga ccatttttat ctatttgatt
    57901 cacccagggg gaaaacttgc agggtaaagc actccaggat tttgttatcg acaaaattga
    57961 tgacctcaaa ggtcaggaca tcatcgcctt agacgttcaa ggcaaatcca gcatcaccga
    58021 ctgcatgatc atctgtacgg gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt
    58081 tgtgcaggag tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc
    58141 cgactggatt gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg aagagagccg
    58201 tcgcctgtat gaactggaaa aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt
    58261 gggaacgaaa atgccggact gggtacaaac cggttttacc gagtacctgc gtcgttttcc
    58321 gaaagatatg cccttcgagc tgattgaaat tccggccggc aaacgcggca agaatgcgga
    58381 catcaagcgc atactcgaca aagagggtga gcagatgttg gcggccgcag gcaaaaaccg
    58441 cattgtcacc ctcgatattc caggcaagcc ctgggatacg ccgcagttag ccgctgagct
    58501 ggaacgctgg aagctggatg gtcgcgacgt aagtctactg attggcgggc ctgaagggtt
    58561 gtcgcctgcc tgtaaagcgg cggctgagca gagctggtcg ctgtcggcgc ttaccctccc
    58621 ccatccgctg gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac
    58681 caaccatcct tatcaccgtg agtgataagg gagctttgag tagaaaacgc agcggatgaa
    58741 actacagaac tcttttcgcg actatacggc agagtccgcg ctgtttgtgc gccgggcgct
    58801 ggtcgccttt ttggggattt tgctgctgac cggcgtgctt atcgccaacc tgtataatct
    58861 gcaaattgtt cgctttaccg actaccagac ccgctctaat gaaaaccgca ttaagctggt
    58921 gcctatcgcg cccagccgcg gcattatcta cgaccgtaac ggtatccctc tggccctcaa
    58981 ccgcactatc taccagatag aaatgatgcc ggagaaagtc gataacgtgc agcaaacgct
    59041 ggacgctttg cgcagcgtgg tagatctgac cgatgacgat attgctgcat tccgaaaaga
    59101 gcgcgcacgt tcacaccgtt tcacctctat tccggtgaaa actaacctaa ccgaagtaca
    59161 agtagctcgc tttgccgtca atcagtaccg ttttccgggt gtcgaagtta aaggctataa
    59221 acgtcgttac tatccttacg gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat
    59281 caacgataaa gacgtcgaac gcctgaataa tgacggcaaa ctggccaact atgcggcaac
    59341 gcatgatatt ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc acggtcagac
    59401 cggttatgaa gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt
    59461 accaccgcaa gccggacacg atatttacct gacgctggat ctcaaactcc agcaatatat
    59521 tgaaacgctg ctggcgggta gccgcgcagc tgtggttgtt accgaaccgc gtacaggtgg
    59581 ggtgctggcg ctggtttcca cgcctagtta tgacccaaac ctgtttgttg acggtatctc
    59641 cagcaaagat tattccgcct tgctgaacga cccgaataca ccgctggtga accgcgccac
    59701 acagggggtt tatcctcccg cgtctacagt taaaccctat gtggcggttt cggcattaag
    59761 cgccggggtg atcacgcgca atacgacgct gtttgaccca ggctggtggc aactaccagg
    59821 gtcggaaaaa cgttatcgtg actggaaaaa atggggccac gggcgtctga atgtcacaag
    59881 atcgctggaa gaatctgcgg ataccttctt ctatcaggtg gcctacgata tggggatcga
    59941 tcgcctctcc gaatggatgg gtaaattcgg ttatggtcat tacaccggta tcgacctggc
    60001 ggaagaacgt tccggcaaca tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc
    60061 gtggtatcag ggtgacacca ttccggttgg tatcggccag ggttactgga cagcgacccc
    60121 aatccagatg agtaaggcac tgatgatcct gattaatgac ggtatcgtga aggttccgca
    60181 tttgctgatg agcaccgccg aagacggcaa acaggtgcca tgggtacaac cgcatgaacc
    60241 gcccgtcggc gatattcatt ccggttactg ggagctggcg aaagacggta tgtacggtgt
    60301 tgctaaccgc cctaatggta cggcgaataa atactttgcc agcgcaccgt acaaaattgc
    60361 ggcgaaatct ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca
    60421 caaaattgcc gagcgtttac gtgaccacaa actgatgacc gcctttgcgc catacaacaa
    60481 tccgcaagtg gctgtcgcca tgattctgga gaacggcggc gcgggtccgg cggttggtac
    60541 gctgatgcgc cagatcctcg accacattat gctgggtgat aacaacaccg atctgcctgc
    60601 ggaaaatcca gcggttgccg cagcggagga ccattaatca tgacggataa tccgaataaa
    60661 aaaacattct gggataaagt ccatctcgat cccacaatgc tgctgatttt actggcattg
    60721 ctggtttaca gcgccctggt tatctggagc gccagcggtc aggatattgg catgatggag
    60781 cgtaaaatcg gccaaatcgc gatgggtctg gtcatcatgg tggtgatggc gcaaattcct
    60841 ccacgcgttt atgaaggctg ggccccctat ctctatatca tctgtattat tttgctggtg
    60901 gcggtagatg ctttcggagc catctctaaa ggcgctcaac gctggctgga cctcggtatt
    60961 gttcgttttc agccgtcgga aattgccaaa atagccgtac cactgatggt tgcgcgcttt
    61021 atcaaccgcg acgtttgccc gccatcgttg aagaatactg gcatcgcgct ggtgctgata
    61081 tttatgccca cgctgctggt ggctgcacag cctgacctgg gaacatcaat cctcgttgcg
    61141 ctttccggtc tgtttgtact gttcctctct ggccttagct ggcgtctgat tggcgtcgca
    61201 gtagtgctgg tagcggcgtt cattccgatt ctgtggttct tcctgatgca tgattaccag
    61261 cgccagcgcg taatgatgct cctggacccg gaatcagacc cactcggcgc gggctatcac
    61321 attattcagt ctaaaattgc tattggctcc ggcggattac gcggcaaagg ctggctgcac
    61381 ggcactcagt cacagcttga atttctcccc gaacgccata ctgactttat cttcgcggta
    61441 ctggcggaag agctgggatt agtgggcatt ctgattctgc tcgctctcta cattctgctg
    61501 atcatgcgcg ggctgtggat agccgccaga gcgcaaacca cctttggtcg cgtcatggct
    61561 ggtggcttaa tgctgatatt attcgtttat gtcttcgtaa atattggtat ggtaagcggt
    61621 attctgccgg ttgtaggggt tccgctccca ctggtcagtt atggaggatc ggcgctaatt
    61681 gtgctgatgg ctgggttcgg gattgtaatg tcaatccaca cccacaggaa aatgttgtcg
    61741 aaaagcgtgt aagaggtgcg caatgcgtaa gcagtggctc gggatctgca tcgcggcagg
    61801 aatgctcgcg gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg taccgcagcc
    61861 tgcggtatgt aacggcccca tagttgaaat tagcggggcg gacccgcgtt tcgaaccact
    61921 gaacgcgacg gcaaatcagg attaccagcg cgacggtaaa agctacaaaa tcgtgcagga
    61981 tccgtctcga tttagccagg cgggactggc ggcaatctat gatgccgaac caggcagtaa
    62041 cctgacggcc tctggcgaag ctttcgatcc gacaaagctg acggcggccc atccaacgct
    62101 tccgatcccc agctacgcca gaatcactaa cctggctaac gggcgaatga tcgtggtgcg
    62161 cattaatgat cgcggccctt acggcaacga ccgcgttatt tcgctttctc gcgcggcagc
    62221 tgaccgtctt aacacgtcaa acaacaccaa agttcgtatc gatccgatta ttgtcgccca
    62281 ggatggttcg ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac agacttacgc
    62341 cctgcctgca cctcccgatt taagcggtgg cgcgggaaca agttcagtgt ctggcccgca
    62401 gggtgacatt cttccggtca gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc
    62461 ggtaaccagt agcggtttcc tcggcgctcc aacgacctta gcgcctagtg tactggaagg
    62521 cagcgaaccg acgcctgcgc cacagcccgt tgttacagct tcgtcgacaa cgcctgcaac
    62581 ctcgcctgca atggtgacac cgcaagccgc ctcgcaaagc gccagcggca actttatggt
    62641 gcaagtcggg gccgtaagcg atcaggctcg tgcgcaacag taccaacagc aactgggaca
    62701 gaagttcggc gtccccggtc gcgtaactca aaatggcgcg gtctggcgga tccagcttgg
    62761 cccattcgcc agcaaagccg aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca
    62821 attacagtca tttattacta ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt
    62881 ctgtaaatga cgttaacaaa gtcacgcgga aagtcagatg cctgccggta gtgcatttgc
    62941 tatagtaggg cactttttta attccatcac ggatgtcgta gttcagacca tgaataccat
    63001 tttttccgct cgtatcatga agcgcctggc gctcaccacg gctctttgca cagcctttat
    63061 ctctgctgca catgccgatg acctgaatat caaaactatg atcccgggtg taccgcagat
    63121 cgatgcggag tcctacatcc tgattgacta taactccggc aaagtgctcg ccgaacagaa
    63181 cgcagatgtc cgccgcgatc ctgccagcct gaccaaaatg atgaccagtt acgttatcgg
    63241 ccaggcaatg aaagccggta aatttaaaga aactgattta gtcactatcg gcaacgacgc
    63301 atgggccacc ggtaacccgg tgtttaaagg ttcttcgctg atgttcctca aaccgggcat
    63361 gcaggttccg gtttctcagc tgatccgcgg tattaacctg caatcgggta acgatgcttg
    63421 tgtcgccatg gccgattttg ccgctggtag ccaggacgct tttgttggct tgatgaacag
    63481 ctacgttaac gcactgggcc tgaaaaatac ccacttccag acggtacatg gtctggatgc
    63541 tgatggtcag tacagctccg cgcgagatat ggcgctgatc ggccaggcat tgatccgtga
    63601 cgtaccgaat gaatactcga tctataaaga aaaagaattt acgtttaacg gtattcgcca
    63661 gctgaaccgt aacggcctgt tatgggataa cagcctgaat gtcgacggca tcaaaaccgg
    63721 acacactgac aaagcaggtt acaatcttgt tgcttctgcg actgaaggcc agatgcgctt
    63781 gatttctgcg gtgatgggtg gacgtacttt taaaggccgt gaagccgaaa gtaaaaaact
    63841 gctaacctgg ggcttccgtt tcttcgaaac cgttaaccca ctgaaagtag gtaaagagtt
    63901 cgcctctgaa ccggtttggt ttagtgattc tgatcgcgct tcgttagggg ttgataaaga
    63961 cgtgtacctg accattccgc gtggtcgcat gaaagatctg aaagccagct atgtgctgaa
    64021 cagcagtgaa ttgcatgcgc cgctgcaaaa gaatcaggtc gtcggaacta tcaacttcca
    64081 gcttgatggc aaaacgatcg agcaacgccc gctggttgtg ttgcaagaaa tcccggaagg
    64141 taacttcttc ggcaaaatca ttgattacat taaattaatg ttccatcact ggtttggtta
    64201 aaaattgaac acttgaaagt gtaatttccg tccccatata ctaagcatca gtaaaaaaac
    64261 tcccgccttc tggcgggagt tgctatttaa ttacgttacg ccggagctga catgaaaacc
    64321 aaacttaacg aactgcttga attccctact ccttttactt acaaagttat ggggcaggcg
    64381 ttacctgagc tggttgatca ggtggttgaa gtggtacagc gccatgcgcc aggtgactac
    64441 accccaacgg taaaaccaag cagcaaaggc aactaccact cggtatctat cactatcaac
    64501 gccactcata tcgagcaggt tgaaacactg tatgaagaac tgggcaaaat cgatattgtc
    64561 cgcatggttc tgtaactcgc ttctccgtta cccggctcct ggtcgggtaa ctcccctctc
    64621 ctcgctgtga tatacttttc ccccactttt actcattctc cacggagatg ccgttttgta
    64681 tcaggataaa attcttgtcc gccagctcgg tcttcagcct tacgagccaa tctcccgggc
    64741 tatgcatgaa ttcaccgata cccgcgataa tagtaccctt gatgaaatct ggctggtcga
    64801 gcactatccg gtattcaccc aaggtcaggc aggaaaagcg gagcacattt taatgccggg
    64861 tgatattccg gtgatccaga gcgatcgcgg tgggcaggtg acttatcacg ggccgggaca
    64921 acaggtgatg tatgtgttgc ttaacctgaa acgccgtaaa ctcggtgtgc gtgaactggt
    64981 gaccttgctt gagcaaacag tggtgaatac cctggctgaa ctgggtatag aagcgcatcc
    65041 tcgggctgac gcgcctggtg tctatgtcgg ggaaaagaaa atttgctcac tgggtttacg
    65101 aattcgacgc ggttgttcat tccacggtct ggcattaaac gtcaatatgg atctttcacc
    65161 atttttacgt attaatcctt gtgggtatgc cggaatggaa atggctaaaa tatcacaatg
    65221 gaaacccgaa gcgacgacta ataatattgc tccacgttta ctggaaaata ttttagcgct
    65281 actaaacaat ccggacttcg aatatattac cgcttaattc catacatcaa tggcccaatt
    65341 tacactgggt cattactcat aatacctgct aatgctttac atctccttcg cgtttgtata
    65401 ttttctttat cacttgcaga aatgcggtgc cacgccttgc tccgtaactc accccattga
    65461 ttaacctatt gaaatagttg attatggtga gcaaaagcgt tccgttccaa ttagacataa
    65521 gattattgta attttttaaa tattattttc ccatgaataa aattggagta agtgcgtgga
    65581 tagtaataat caaattgaac cctgtttaag cagaaagtcg tcagaaggta aaccacaaat
    65641 atttacaacc ttacgaaata ttgatcttaa ccttctgact atttttgaag ctgtatatgt
    65701 acataaaggg atcgttaatg cagcgaaagt gcttaatctg accccctcgg caatcagtca
    65761 gtctattcag aaactgcgcg ttatattccc tgacccattg tttattcgca aaggccaggg
    65821 tgtcactcct accgcatttg cgatgcatct acatgagtat atcagtcagg gccttgagtc
    65881 cattcttggc gcgctggata tcgaaggaag ctatgataag caacgaacga taactattgc
    65941 gaccactccc tcggtcggag ccctggtcct tcctgtcatc tatcgggcga ttaaaactca
    66001 ctatccgcag cttttactgc gcaacccgcc catcagcgac gcggaaaacc aactcagtca
    66061 gtttcaaacc gatctcatca tcgataacat gttttgcacc aatcgtacgg tgcaacatca
    66121 tgttctgttc accgacaata tggtgttaat ttgccgcaag ggaaatccac tactctcttt
    66181 agaagatgac agagagacta tcgacaacgc tgcgcatgta cttctgttac cggaagggca
    66241 aaatttcagc ggtctgcggc agagagttca agagatgttt ccggaccggc aaatcaattt
    66301 caccagctac aacattttga caatcgctgc actggttgcc aacagtgaca tgttagcgat
    66361 tattcccagc cgtttttata acctgtttag tcgttgctgg ccgctggaaa aactgccttt
    66421 tccgtcctta aatgaggagc aaatagattt ctctatccac tacaacaaat tcatcctgcg
    66481 tgatcccatc ctgcacggag taatagatgt tatccgtaat gcattttgaa aaagtattgt
    66541 tacagccaga tcacagcgca gaacgataaa atggcacaat ccaacaacaa ttttacattt
    66601 tcgcgaccgc tttggctgct ttcaggtccg tttcaatgat atactgccag tcgttaattc
    66661 aaaaatagtt gataattaca acaatctatt gaattgaaac gctttccttc gtaattcgca
    66721 actggaacac gcacgctatg agtaaaccca ttgtgatgga acgcggtgtt aaataccgcg
    66781 atgccgataa gatggccctt atcccggtta aaaacgtggc aacagagcgc gaagccctgc
    66841 tgcgcaagcc ggaatggatg aaaatcaagc ttccagcgga ctctacacgt atccagggca
    66901 tcaaagccgc aatgcgcaaa aatggcctgc attctgtctg cgaggaagcc tcctgcccta
    66961 acctggcgga atgcttcaac cacggcacag caacgtttat gatcctcggc gctatttgta
    67021 cccgccgttg tccgttctgt gatgttgccc acggtcgccc ggtagctcct gatgccaatg
    67081 aaccagtgaa actggcgcag accattgccg atatggcgct gcgttatgtg gttatcacct
    67141 ccgttgaccg tgatgacctg cgcgatggcg gtgcccaaca ctttgcggat tgtattactg
    67201 ccattcggga aaaaagcccg caaatcaaaa ttgaaactct ggtgccggat ttccgcggtc
    67261 gaatggatcg tgctctggat attctgactg caacgccccc agatgtgttc aaccataacc
    67321 tggaaaacgt accgcgtatt taccggcagg tacggcctgg cgcagattac aactggtcgc
    67381 tgaagctgct ggaacgcttt aaagaagcgc atccggaaat cccgaccaag tctggtctga
    67441 tggtgggtct gggtgaaacc aatgaagaaa ttattgaggt aatgcgcgac ctgcgccgtc
    67501 atggtgtgac gatgttaacg ctggggcaat atttgcagcc aagccgccat cacctgccgg
    67561 ttcaacgtta cgttagcccg gatgagttcg acgaaatgaa agccgaagcg ctggcgatgg
    67621 gctttaccca tgctgcatgc ggtccgtttg tccgctcttc ttaccacgcc gatttgcagg
    67681 cgaaaggaat ggaagttaag taatcctgta ataattactt acatctgtct gataaaaaac
    67741 ccgctttttg aagcgggttt tttgtatcga acaagatatt gagggagcgt cctgctcgcc
    67801 acgtcactct ttatgagaga gcttttcagc ctgaagatca acgtctgcgc cttttttcgc
    67861 cgcagcatcg tcatcattca tcgccttctt gaacccttta atggccgctc caaggtctcc
    67921 gcccagcgta cgtaacttct tagtcccaaa cagcagaacg accagcgccg caactaccag
    67981 cagtttggta atactaatct cacccataga taccttcttg acatataacc ggcaacagaa
    68041 cgcgccttaa aaaccagaga atattaacgg tcattcgacg cgcgcacaca atagcaatct
    68101 gtaacaaggt gaatcaagcg ccgagtaaaa aaacatcata ataattgcgg cggcgcaaag
    68161 cgacggttgt ttaagatggg cagttgcgcg cgcacctgac gcacacgttc gggcgtcact
    68221 tccgccataa tgagtgcagg catttctgac gctgccgcaa tggtgacgcc aaagggatct
    68281 ataatccggc tttgcccgat atttttgttc ccgcactccc ccgccgccac catataacag
    68341 gtggtatcca gcgcacgagc ggcaagcaac gttgaccagt gatgctcttt gagcggccca
    68401 cgaacccagg cggcaggaag taccaggatt tcagctccct gtaaagcctg tgccagcgcc
    68461 agctctggaa agcgtaagtc ataacaggtc atcagaccga ccttcatccc ttccacctcc
    68521 agtaacggag ctatttcatt accagcatca acatggcgtg attcctgaat ggcaaatgca
    68581 tcatagagat gcagtttggc ataacgggcg acgatgtttc ctgcctgaag tgccaccagc
    68641 atattccatg cgcgccccgg cgttgaagga agatgaatcg tcagaattgt cgtcatcatg
    68701 ttacgtttac tttctcgccg taaacgtccg aggaattcgc cttccagcag ctgtgctgat
    68761 ttaaccgata gatctgcatc atgatcatcg cgcgccagca atgcttccgg caggacaaac
    68821 agcgatacgt cgttttccgc cgcctgcgcc atcaacgagg cacaaatctc agcgttcttt
    68881 tcccacacag atgtaacagc aaactgtcct gcagcaacca gcattgccac ctcctgtcag
    68941 agtcgtaaaa ttcgccaacg acgattattc ggcgttacac ttatcactca tacaaatcaa
    69001 atagcaggat tttgcagtgt tacaacttct tttagcagtt tttattggcg gtggtacggg
    69061 aagcgtagcg agatggctgt taagtatgcg atttaacccg ctgcatcagg cgattccgtt
    69121 ggggacgctg gcagcaaacc tgattggggc attcatcata ggaatgggat tcgcctggtt
    69181 cagcaggatg acgaacattg atccagtgtg gaaagtatta atcaccaccg gattttgtgg
    69241 cggtctaaca accttctcaa cattttcggc agaagtggtg tttttgttac aagagggccg
    69301 ctttggctgg gcattactga acgttttcgt caaccttctg gggtcttttg ccatgaccgc
    69361 actggcattc tggctgtttt cggcctcaac cgtacactaa agaccaaaaa aaacccgctg
    69421 attaagcggg ttttgaattc ttgctgacgt atcttacaga gcgattacgt ttgcagcaga
    69481 agggcctttg gcaccgttag tgatttcgaa ctctacgcgc tgaccttcag caagagtttt
    69541 aaaaccatta gtctggattg cagagaagtg tacgaacacg tctttgctgc cgtcttccgg
    69601 agtaatgaaa ccgaatcctt tggactcatt aaaccactta acgttacctt taatcttaga
    69661 catcaaaatt acctttacat gaaaaataga cacaaatgct gtgtcggtta tcagtacacc
    69721 aattgtggta cgctttgtcc agtgcaactt tactaaaaag tgataaaagt cgcgttattt
    69781 ttgatgttgc ttattttagt tattgatttt aaagaagtta ctaaaacttc atttgtctca
    69841 aaactgaaag cgcatccagg caaagtacac attgccattg ttgtaggtac ccggaatgta
    69901 ggtcatctga aaagtcactg ggccataacc cacggaggcc aatggcagta gaaccgggag
    69961 agggatgtaa ttccagttat cgcgtgccgt tacgccagcg gtgaatccca gacctaaatg
    70021 aaaattttca tccgccagcg gtcgccaggt actttcccat ccgtatccgg caatcggttc
    70081 ccatttgttc cacgagtcct taaatgccat ggcatacagg ccatgccagt ttcctttttc
    70141 atcccaacgc gacaggccaa aaccgccacc ccacggtcgc tcgttatagc gatcggtttt
    70201 ttctttgtcg taagcgaaac gtgcatgcca ggtgatggca ggaatatata aatcataatg
    70261 ttcaggctgt tgccaggttt gtgcaatatt ttctctaaac gttgtcatcc tctcatctgc
    70321 gttagcaaaa actttaccaa cgctgattaa ctgaataaaa acaaaggaaa agatagcgac
    70381 atatttactc acgttcattt gtgaccataa aacatttatc aaaaatctac tactagcata
    70441 gcaaagctac aattaacata accttaatag acccaacata aagaataatc tgaatagctg
    70501 ctctatttat ttccgcttgt tcaagttaaa tatttccgcg catcgattaa agatgatgcg
    70561 tcgttttctg cgatgggaat agtcaaaaaa gaaaaaccaa gtcttttttg atgacaaaat
    70621 gcaatcaagg aaaaattatt ttattttttt aacgagatac aacaatcatc ttaacgaagt
    70681 atataatata tatcgtcaac catgctcata ttagattatt ttttgagcgc acagccgcag
    70741 ctaacaccat tgcaattaac aaatttgcat caatccacca tcaatttaca cacattatta
    70801 tgtgataatt gccaaccgct aaatatgcgt tttgttatct atgtataaaa acagcaactt
    70861 caatgtctta atggcagttt ttcttgattt taatcagcat tcatcgccaa tttattgggc
    70921 atattttttc cttaagcttt aggaagtttt tattatttac tttgggcctg gagacaggaa
    70981 aaattatgct gacattcatt gaactcctta ttggggttgt ggttattgtg ggtgtggctc
    71041 gctacatcat taaagggtat tccgccactg gtgtgttatt tgtcggtggc ctgttattgc
    71101 tgattatcag tgccattatg gggcacaaag tgttaccgtc cagccaggct tcaacaggct
    71161 acagcgccac ggatatcgtt gaatacgtta aaatattact aatgagccgc ggcggcgacc
    71221 tcggcatgat gattatgatg ctgtgtggct ttgccgctta catgacccat atcggcgcga
    71281 atgatatggt ggtcaagctg gcgtcaaaac cattgcagta tattaactcc ccttacctgc
    71341 tgatgattgc cgcctatttt gttgcttgtc tgatgtcact ggccgtctct tccgcaaccg
    71401 gtctgggtgt tttgctgatg gcaaccctgt ttccggtgat ggtaaacgtt ggtatcagtc
    71461 gtggcgcagc agctgccatt tgtgcctccc cggcggcgat tattctcgca ccgacttcag
    71521 gggatgtggt gctggcggcg caggcttccg aaatgtcgct gattgacttc gccttcaaaa
    71581 cgacgctgcc tatctcaatt gctgcaatta tcggcatggc gatcgcccac ttcttctggc
    71641 aacgttatct ggataaaaaa gagcacatct ctcatgaaat gttagatgtc agtgaaatca
    71701 ccaccactgc ccccgcgttt tatgccattt tgccgttcac gccgatcatc ggtgtactga
    71761 tttttgacgg taaatggggt ccgcaattac acatcatcac tattctggtg atttgtatgc
    71821 tgattgcctc cattctggag ttcctccgca gctttaatac ccagaaagtt ttctctggtc
    71881 tggaagtggc ttatcgcggg atggcagatg cgtttgctaa cgtggtgatg ctgctggttg
    71941 ccgctggggt attcgctcag gggcttagca ccatcggctt tattcaaagt ctgatttcta
    72001 tcgctacctc gtttggttcg gcgagtatca tcctgatgct ggtattggtg attctgacca
    72061 tgctggcggc agtcacgacc ggttcaggca atgcgccgtt ttatgcgttt gttgagatga
    72121 tcccgaaact ggcgcactct tccggcatta acccggcgta tttgactatc ccgatgctgc
    72181 aggcgtcaaa cctgggtcgt accctatcac ccgtttctgg cgtagtcgtt gcggttgccg
    72241 ggatggcgaa aatctcacca tttgaagtcg taaaacgcac ctcggtgccg gtgcttgttg
    72301 ggctagtgat tgttatcgtt gctacagagc tgatggtgcc aggaacggca gcagcggtca
    72361 cagacaagta aatagtaatg ccggaggcaa gtttcctccg gctttatcat gtcacccact
    72421 gtggtatatg cgttgtggtc tgccaacttt gccgtgaaca atttcagcaa taatcagatg
    72481 gcggctggcg caatattcaa gataacgcct ggcagtggtg cggctgatgg tcagtgcctg
    72541 cgccaccgtt tctgccgtat gttgcacacc aggctcttta aacagttttc gcaccgcgtt
    72601 tagcgtcaag gggtcaatgc cggtcggcag ttcgtctttg ggttcaccgc gagcataggc
    72661 attaaacatc tcatcaattt gcttctggct ggcgctatca atactttcca gcatatgttt
    72721 acgctggcgg aaacgggtta gcgtttgccc cagtgtattg attcacttga agtacgaaaa
    72781 aaccgggaga acattggatt attcgggatc tgatgggatt agatttggtg gggcttgcaa
    72841 gcctgtagta caaattttag gcgttaacca atgaaacgcg aaagatagta aaaaattgct
    72901 tttgtttcat tgaaaaaacg aaaaacaaaa acactataaa tcatttcaat catagcttca
    72961 aaaaacgttc aaaaccgata acaaccaggc tgtcaccaaa tgactcatat cacaaatcag
    73021 ctcatgccgt ttaggtatgt tacatgtgtg attatgtgag gtgaagtatg ttttagctgg
    73081 ctcatggttg ttatacggct tttttcgcat cttgtggttc caacaaagga gctacaggct
    73141 cttcctgttt atgaacacca tacattgaca aaatctcttg gcgagtttct ggaggtaatt
    73201 tatgcagcgc caaagctgtt tgtagtgttt ggcgatcaat acccagcgtt gccaataggg
    73261 tgtcaacgga gatggagggg cgttcttgca ttaatccgga aagcccatat ttagccatgt
    73321 ttagctctat ctctcggact tcattttctc ctagtttatt gattaacagg gagaacattt
    73381 tcaatgtatc agcattcatt tcttcgatag caattttttg catagccggt attgccggga
    73441 tatattcaac cccaggcccg cgacgccctt cttgtgaacg cttcggccat ccttctttct
    73501 ttgctcgttt atgcaccccc tgtattgtcc caggcattcc atccagttca gtaatttgtt
    73561 tgggtgtcat ccactggttt atctcatttg cgcttttcat aaactaagtt taccagattt
    73621 atctatataa aaatcaatgg attacaaaat aaaccacatt aactgatata aaccggtttt
    73681 gtttatttgg taatgtataa aacagataat caagacgaat tatttgtttt ggtaaccctt
    73741 tatcatacgc aatatggttg gtgtgcgaaa gatgagtaac gattggcatc gggccgacgt
    73801 gattgcggcc ctcaaaaaaa caggaacccc tctggcaaaa ttgtccagac gttcaggtta
    73861 ttcaaccggg acgcttgcaa atgcattagt caggccatgg cctaaaggtg aacgcattat
    73921 tgctgacgcc ttaaacatga aacctgagga aatttggcct agtagatatc taagggatca
    73981 caatgagaga gtggatatca gcaattgaat gcatgggttt atctggttta cctggttcat
    74041 taatgggggt tcacaaaaga gctcagagag aaggatggct caagagaaga agagaaggaa
    74101 ttagaggggt tgcttacgaa taccatatat cttctcttcc taacattgct cgagctgaag
    74161 ttttgctcca acaaggaaaa tttaaaacca gtcagggatg ttttgaaatc gcccgcccca
    74221 cgctggaagc tcatgattat gatcgtgagg cactatggag caaatgggat aaagccagcg
    74281 attcccagcg cagcctggct gaaaaatggt tgccctcaat tcaggccaca gacgaaatgc
    74341 tgaaccaggg gatttcaacg aaaacggctt ttgcgaccgt tgcagggcat taccaggtca
    74401 gcgcatccac tttgcgggac aagtattacc aggtacagaa gtttgcgaag cctgactggg
    74461 cggctgcact tgttgatgga cgtggagcat cccgtcgcaa tgttcacaaa agtgaatttg
    74521 acgaggatgc ctggcagttt ctgattgcag attatctgcg accggaaaaa cccgccttcc
    74581 gcaaatgtta tgagcgtctg gaactggcag cccgcgagca tggctggagt attccctccc
    74641 gtgccacggc ctttcgccgg attcagcaac tggacgaggc aatggttgtt gcctgtcgtg
    74701 aaggtgaaca tgcactgatg catctgatac cggcacagca gcgaactgtg gaacacctgg
    74761 acgccatgca gtggatcaac ggcgacggtt atctgcataa cgtctttgta cgctggttta
    74821 acggtgatgt gatccgtccg aaaacatggt tctggcagga tgtgaaaacc cgaaaaattc
    74881 tgggctggcg ctgcgatgtg agcgagaaca ttgattcaat tcgcctctcg ttcatggatg
    74941 ttgtgactcg ctacagtatc ccggaggatt ttcacatcac cattgataac acccgtggtg
    75001 ctgcgaataa atggctgacg ggaggcgcgc ccaatcgcta ccgctttaag gtaaaagagg
    75061 acgatccaaa aggactgttt ttactgatgg gtgcgaaaat gcactggaca agcgttgttg
    75121 ccggtaaagg ctggggccag gcaaaacctg ttgaacgtgc tttcggtgtt ggtgggcttg
    75181 aggaatacgt tgataagcat ccggcactgg ctggcgcata tacggggcca aatccgcagg
    75241 caaaacctga taactatggc gaccacgctg ttgatgcaga gctgtttctg aaaacctttg
    75301 ccgaaggtgt ggcgatgttc aatgccagaa caggccgtga aacagaaatg tgcgggggca
    75361 aactctcgtt tgatgatgtt ttcgagcgtg aatacgccag aacgattgtg cgtaagccaa
    75421 ccgaagaaca aaaacggatg ctgttactgc ctgccgaggc ggtgaacgtt tcacgcaaag
    75481 gcgagtttac gcttaaagtt ggcggctccc ttaaaggcgc gaaaaacgtt tattacaaca
    75541 tggcattaat gaatgccgga gtgaaaaaag ttgtggtcag atttgatcca cagcagttac
    75601 acagcacggt ttattgag
//
LOCUS       DAEEGA010000005        72935 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEEGA010000005 DAEEGA010000000
VERSION     DAEEGA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72935
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.005"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="KLH46_000323"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="KLH46_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBE0377148.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="KLH46_000324"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="KLH46_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBE0377149.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="KLH46_000325"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="KLH46_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBE0377150.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="KLH46_000326"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="KLH46_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBE0377151.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="KLH46_000327"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="KLH46_000327"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBE0377152.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="KLH46_000328"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="KLH46_000328"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBE0377153.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDNLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="KLH46_000329"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="KLH46_000329"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBE0377154.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="KLH46_000330"
     CDS             complement(4598..5320)
                     /locus_tag="KLH46_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE0377155.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="KLH46_000331"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="KLH46_000331"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBE0377156.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="KLH46_000332"
     CDS             6520..6738
                     /locus_tag="KLH46_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBE0377157.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="KLH46_000333"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="KLH46_000333"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBE0377158.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="KLH46_000334"
     CDS             complement(7472..7672)
                     /locus_tag="KLH46_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBE0377159.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="KLH46_000335"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="KLH46_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="KLH46_000336"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="KLH46_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBE0377160.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="KLH46_000337"
     CDS             10101..12014
                     /locus_tag="KLH46_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0377161.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="KLH46_000338"
     CDS             12014..13036
                     /locus_tag="KLH46_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBE0377162.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="KLH46_000339"
     CDS             13030..13248
                     /locus_tag="KLH46_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBE0377163.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="KLH46_000340"
     CDS             13302..14171
                     /locus_tag="KLH46_000340"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBE0377164.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="KLH46_000341"
     CDS             complement(14226..14630)
                     /locus_tag="KLH46_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBE0377165.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="KLH46_000342"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="KLH46_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBE0377166.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="KLH46_000343"
     CDS             15615..17705
                     /locus_tag="KLH46_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBE0377167.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="KLH46_000344"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="KLH46_000344"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBE0377168.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="KLH46_000345"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="KLH46_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBE0377169.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="KLH46_000346"
     CDS             complement(19673..20275)
                     /locus_tag="KLH46_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBE0377170.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="KLH46_000347"
     CDS             complement(20265..20432)
                     /locus_tag="KLH46_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBE0377171.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVILTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="KLH46_000348"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="KLH46_000348"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBE0377172.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="KLH46_000349"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="KLH46_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBE0377173.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="KLH46_000350"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="KLH46_000350"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBE0377174.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGSIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="KLH46_000351"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="KLH46_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBE0377175.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="KLH46_000352"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="KLH46_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBE0377176.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="KLH46_000353"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="KLH46_000353"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBE0377177.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="KLH46_000354"
     CDS             28269..28436
                     /locus_tag="KLH46_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBE0377178.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="KLH46_000355"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="KLH46_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="KLH46_000356"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="KLH46_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBE0377179.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="KLH46_000357"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="KLH46_000357"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HBE0377180.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="KLH46_000358"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="KLH46_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBE0377181.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="KLH46_000359"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="KLH46_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBE0377182.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="KLH46_000360"
     CDS             complement(33764..34849)
                     /locus_tag="KLH46_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBE0377183.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPGGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="KLH46_000361"
     CDS             complement(34861..36165)
                     /locus_tag="KLH46_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBE0377184.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="KLH46_000362"
     CDS             complement(36177..36530)
                     /locus_tag="KLH46_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBE0377185.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="KLH46_000363"
     CDS             complement(36541..37419)
                     /locus_tag="KLH46_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBE0377186.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="KLH46_000364"
     CDS             complement(37416..38642)
                     /locus_tag="KLH46_000364"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBE0377187.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="KLH46_000365"
     CDS             complement(38643..39806)
                     /locus_tag="KLH46_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBE0377188.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="KLH46_000366"
     CDS             complement(39890..40252)
                     /locus_tag="KLH46_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBE0377189.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="KLH46_000367"
     CDS             complement(40269..41174)
                     /locus_tag="KLH46_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377190.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="KLH46_000368"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="KLH46_000368"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBE0377191.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="KLH46_000369"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="KLH46_000369"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBE0377192.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="KLH46_000370"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="KLH46_000370"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBE0377193.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="KLH46_000371"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="KLH46_000371"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBE0377194.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46116)
                     /gene="damX"
                     /locus_tag="KLH46_000372"
     CDS             complement(44830..46116)
                     /gene="damX"
                     /locus_tag="KLH46_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBE0377195.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDSAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46208..47296)
                     /gene="aroB"
                     /locus_tag="KLH46_000373"
     CDS             complement(46208..47296)
                     /gene="aroB"
                     /locus_tag="KLH46_000373"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBE0377196.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47353..47874)
                     /gene="aroK"
                     /locus_tag="KLH46_000374"
     CDS             complement(47353..47874)
                     /gene="aroK"
                     /locus_tag="KLH46_000374"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBE0377197.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48275..49513)
                     /gene="hofQ"
                     /locus_tag="KLH46_000375"
     CDS             complement(48275..49513)
                     /gene="hofQ"
                     /locus_tag="KLH46_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBE0377198.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49425..49829)
                     /gene="hofP"
                     /locus_tag="KLH46_000376"
     CDS             complement(49425..49829)
                     /gene="hofP"
                     /locus_tag="KLH46_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBE0377199.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49819..50259)
                     /gene="hofO"
                     /locus_tag="KLH46_000377"
     CDS             complement(49819..50259)
                     /gene="hofO"
                     /locus_tag="KLH46_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBE0377200.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50243..50782)
                     /gene="hofN"
                     /locus_tag="KLH46_000378"
     CDS             complement(50243..50782)
                     /gene="hofN"
                     /locus_tag="KLH46_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HBE0377201.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50782..51561)
                     /gene="hofM"
                     /locus_tag="KLH46_000379"
     CDS             complement(50782..51561)
                     /gene="hofM"
                     /locus_tag="KLH46_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBE0377202.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51681..54233
                     /gene="mrcA"
                     /locus_tag="KLH46_000380"
     CDS             51681..54233
                     /gene="mrcA"
                     /locus_tag="KLH46_000380"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBE0377203.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54398..54958)
                     /gene="nudE"
                     /locus_tag="KLH46_000381"
     CDS             complement(54398..54958)
                     /gene="nudE"
                     /locus_tag="KLH46_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBE0377204.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55278..57413
                     /gene="igaA"
                     /locus_tag="KLH46_000382"
     CDS             55278..57413
                     /gene="igaA"
                     /locus_tag="KLH46_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBE0377205.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57478..58146
                     /gene="yrfG"
                     /locus_tag="KLH46_000383"
     CDS             57478..58146
                     /gene="yrfG"
                     /locus_tag="KLH46_000383"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBE0377206.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58157..58558
                     /gene="hslR"
                     /locus_tag="KLH46_000384"
     CDS             58157..58558
                     /gene="hslR"
                     /locus_tag="KLH46_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBE0377207.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58583..59452
                     /gene="hslO"
                     /locus_tag="KLH46_000385"
     CDS             58583..59452
                     /gene="hslO"
                     /locus_tag="KLH46_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBE0377208.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59596..61320)
                     /locus_tag="KLH46_000386"
     CDS             complement(59596..61320)
                     /locus_tag="KLH46_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBE0377209.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTPMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61699..63321
                     /gene="pckA"
                     /locus_tag="KLH46_000387"
     CDS             61699..63321
                     /gene="pckA"
                     /locus_tag="KLH46_000387"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBE0377210.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63397..64749)
                     /gene="envZ"
                     /locus_tag="KLH46_000388"
     CDS             complement(63397..64749)
                     /gene="envZ"
                     /locus_tag="KLH46_000388"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBE0377211.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64746..65465)
                     /gene="ompR"
                     /locus_tag="KLH46_000389"
     CDS             complement(64746..65465)
                     /gene="ompR"
                     /locus_tag="KLH46_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBE0377212.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65693..66169
                     /gene="greB"
                     /locus_tag="KLH46_000390"
     CDS             65693..66169
                     /gene="greB"
                     /locus_tag="KLH46_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBE0377213.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66266..68587
                     /locus_tag="KLH46_000391"
     CDS             66266..68587
                     /locus_tag="KLH46_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBE0377214.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69025..69252
                     /gene="feoA"
                     /locus_tag="KLH46_000392"
     CDS             69025..69252
                     /gene="feoA"
                     /locus_tag="KLH46_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBE0377215.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69269..71590
                     /gene="feoB"
                     /locus_tag="KLH46_000393"
     CDS             69269..71590
                     /gene="feoB"
                     /locus_tag="KLH46_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBE0377216.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71590..71826
                     /gene="feoC"
                     /locus_tag="KLH46_000394"
     CDS             71590..71826
                     /gene="feoC"
                     /locus_tag="KLH46_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBE0377217.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72029..72916
                     /gene="rpnA"
                     /locus_tag="KLH46_000395"
     CDS             72029..72916
                     /gene="rpnA"
                     /locus_tag="KLH46_000395"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HBE0377218.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        17579 a        19764 c        18448 g        17144 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaacagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag attgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggtaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattgcacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct ctcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacggatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagaatgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcccgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 gtcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcag catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct agcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 acttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acaagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacattacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tgactgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aagaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgtgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gtcatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggcgcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact gtacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgcccccc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgcgccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaacgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag tacgcttcgg cgtctgtgct accggcttcg gctccgtttt
    45301 cacggttgct tgcggttttt tcggttcaat caccgcctgc tgacgaactg ggcgcgtagt
    45361 ggacggacgt tcagcggttt gcgttttcgc cgagtcacgc gatgcattgc cattgcgaac
    45421 aggcgcaacc gttgcgggtt cagtcggcaa cgtggaattg accgccacat tgttcagctg
    45481 ttgctgattt tgtggctggg tcagcgcatt gttcaggtca ccctgcactt caacacgttg
    45541 ttgaccatcc gttgccaccg gggtttgccc ttgagtcggc gtagaagaga tcggtggcag
    45601 cgaaacatcc tgctgagtat tttccgcaga cgtggttccc ggcgctggct gcacgccatt
    45661 cgcctgatcg gtcgcattgc cagcaagatc aatactcttc tcgccagacg cggtttgatc
    45721 gctggaagtg gtcgaggggg cttttagcgc agaaccgata ccgatgatca acagcagtag
    45781 aaccagaatg ccgacgccca tcatcatata ctgacgagaa gcgggtttac tggctgcttt
    45841 tttgcgctta cgcggacgac gctctacgcg ctcttcatcc acggtttcat cttcggattc
    45901 gtcaatttct tcttcgattt ccggttcctc attgcgctct ttttgcgcac gagtcggacg
    45961 gcgatcgtca gtgtcatcaa gttcaatatc atcaaaattg atctgcggtt cgccacgttc
    46021 agtacgctca gaacgttcag aagattgacg agaacgacca gtacgacgat cgctgggatc
    46081 gggtttcagc tcgtcttctg gtttgaattc atccatttaa caccccacta aaaggttaat
    46141 gcttaccacg ttgcaattaa cctgaagcta atagaccgct tgataagcgg cctgaccttt
    46201 cttgttgtta cgctgattga caatcggcaa tggcgttaag aacaagctcg tgcgaaacgc
    46261 cgctgcgaac ttcactctta ccaattgcca acggaagaat taagcgcatc tctcccgcaa
    46321 ggactttctt gtcacgcagc atatgcggta aatacgcctg cgcggacatt tcgcgcggcc
    46381 cattgaccgg taacccagcc cgcgtgagca gggttataat acgctgcgtt tcggcagaac
    46441 taaactgccc gagacgttcc gacgtccgcg ccgccatcac catacccgca gcgaccgctt
    46501 caccatgtaa ccaattgcca taccccattt cagcttcaat ggcatgacca aaggtgtgtc
    46561 ccagattcag taaagcacgt aacccggttt cgcgctcgtc ggcggcgaca acttctgcct
    46621 tcagttcaca acaacggcga atacagtacg ccattgccgg accgtccaga cgcaacaacg
    46681 catccagatt ctcttccagc cagttaaaaa acgcaccgtc aagaataatg ccgtatttga
    46741 tgacttctgc cagccccgac gctaactcac gcgggggaag cgttttcaga cagtcgagat
    46801 ccaccaccac tgaagcaggc tggtagaacg cgccaatcat gtttttaccg aggggatggt
    46861 tgaccgcagt tttgccgcca acggaggaat cgacctgcga cagtaacgtc gtcgggactt
    46921 gaatgaaacg aacaccgcgc tgataactcg ccgccgcgaa gccggtcaga tcgcccacta
    46981 cgccgccgcc aagcgccacc agcgtagtat cgcgaccatg cggcttttgt aacaacgccg
    47041 taaagacggt atcgagtaca gctaggcttt tatactgctc gccgtcaggg aggataacgc
    47101 tatcgacgtt aacacccgcc tgttcaagta cgccgcggac cttatcgaga tacagaggag
    47161 ccagggtttc gctggtgacc aacatgacct gctcgcccga tttcagcggt aagaatgaag
    47221 ctggttcatt aaacaaacca gatgcgatgg taattgggta actacgttcc ccgagagtaa
    47281 cgacaatcct ctccataacg cgacatccac cttaattact gtacccgcag acgagtgtat
    47341 ataaagccag aattagttgc tttccagcat gtgaataatc tggtttgcaa ccactttagc
    47401 gctttgatca tcagtacgaa tggtcacgtc ggcaatctct tcatacagcg gattgcgttc
    47461 attggccaac gcttccagaa cttcacgcgg cggtgtttca acgtgcagca acgggcgttt
    47521 tttatcacgc tgcgtgcgtg caagttgctt ttcgatggtc gtttcaagat aaacgacaac
    47581 gccacgagcg gaaagacggt tacgcgtttc acgggatttc acagagccgc cgccagtagc
    47641 cagcacaata ccctgtttct cggtcaactc attgatgacc ttttcttcgc gatcgcggaa
    47701 gccttcttcg ccttctaaat cgaaaaccca gcccacatca gctccggttc gtttctcaat
    47761 ctcttgatcg gaatcgtaaa attccatatt gagctgttga gctaactggc gcccaatagt
    47821 gctttttccg gcacccatag gcccaaccag aaagatattg cgtttctctg ccattttttc
    47881 ggtactacta agactattcg ttaatgataa acccgcttcg ctcagagagc gccgcaggac
    47941 atgaactgaa acctcataag atattgcgag agtcagactg aaaattatct caatactcca
    48001 gcgggtttgg caactgaata aatcacccat ccctctgcat atctggtcgc tgcaagcgcg
    48061 ctgccttgct accaccgctc tggcgataaa tcaccgggta agattagcgt aaaaaagaca
    48121 gcaaaatgcc gcctgaatga taaatcatca tcatggaacg caggcagcga agagcaccaa
    48181 ctctcaaatc ggtactcctt gtatgctaaa tacctgcgcg cgtcaaatag atgaaacacg
    48241 ttcagcataa aaacattacc gcttttacgg ctgtttactc actggaaacc agtcgtggcg
    48301 tgataaacac cactaactcg cgtcgttcat cttcttttcc gtcatgacga aataattgcc
    48361 cgaaccaggg aatgtcgcca agcaacggta cgctatcctg acccgattta tttttacggg
    48421 taaaaatgcc gcccagcgcc aacgtttctc cgctttttac ctcgacctgc gtttcgatct
    48481 cctgcttatc aatcgccagc acttcgccat cggcctgctg tagcacctgc cccggaacgt
    48541 tctggctgat gtgtaatttc agccggatgc gacctttttg taacaccgtg ggcgtgacct
    48601 ccatccccag gacggcctct ttaaattcca ccgacgtcgc gccactttcc ccgctggaaa
    48661 cctgatatgg aatttcgctc tcctgtttaa tgctggcagg ctgaagatgt gaggccagca
    48721 gacgcggact ggcgataata tccagctgct gtttttgttc gagcgcggaa agctcaagat
    48781 ccagcaagcg tccgttgatg cgcccaatgt taaaaccgac atgcgttgtc gccgtcgcta
    48841 cggagaggtc gctaccaagc gtggtgactt gcccaacgcc accagcgtgt tgcgcatcgg
    48901 ccagcgtcca tttcacgcct aactcacgca aacttttttc attaatggtg acaatatgcg
    48961 ccgacagctc aacctgcccg accggcagat ccatttgcgc tacccactgt tcaagcgcgc
    49021 ttaacgccgt tttgttatct cgtagcaaaa ggcgattggt gcgtttatcg acggtcatac
    49081 tccctttggc actcagtagc ttctcccccg ctttcgccag ttctcccgcg tcggcgtatt
    49141 gcagggttat actgcgattt tccagcggca gatttgcctg cgcccgcgcc tgctccgcct
    49201 cctggcgggc gatattgtca ttctgccagg caatggaatg cactgagaga atgttgcctt
    49261 cctgccgcgt tatcagtccg gcgcttttca ctacagtttg tagtgcctgc ttccagggaa
    49321 catctgttag atgtaacgac accgtaccgc tgacgtctgg cgacacgacc aggttcaact
    49381 tctcctgttc agccagcgcc tgcaacacct gagctaccgg aacgtcatcc accatcagcg
    49441 tcactttttg cggctttgcc gcctgtacgc cgggtatcag catcaacagt agtgcggcta
    49501 tccattgctt catttgtatc tccttgccgt tgccacaacc attgtggcgg ttcgcagttt
    49561 gtcccggtac ccagcgttag tacgtctggc gtcagctgta aaattgtcca gccgttttcc
    49621 agcacatcgt tttgctgcac ccgtcgccat ttcttttgcc cgtcttttat tacaccgatg
    49681 atgcgctcgc ctcgccctac catcccctga tagcgccact ggctaagttc gctaatccgg
    49741 catagatctt ccggcgattt aaaagggtca cgcataccgg ttaaaaggca caatgcaata
    49801 cctgccaaca accagcgttt aaccctcatg cggcatctcc agttgtagcg tgaacaaaag
    49861 atcatcacct tccacgctta acgaaaaacg gctcacgctg acgttgcgct ctgccagccg
    49921 tgtaaatgcc gacggcactg cttcccacag cgttttcaac gccaactcgc ctccctgcgc
    49981 ggatggatgc caggaaacca gttgcgcgcc ggataactga aaatccagtg gcgaaaaggg
    50041 cagcgttttt tcctcgctga agggagtggt gtctaccagg cgatacagtg cggcccactg
    50101 ctggtgatga cttgcccgta gtcgaattaa tgcgtcgcgc tcttcatggt gtgtcgatga
    50161 tagaaaaatg agcgtaacta acatcagcaa ccagaatgcc cagcaaaact ggcggaggcg
    50221 gggtgatgtg gcgaaccacc agtcaaagaa catgttcatc gctaaccttc cttgttaact
    50281 gatactcaaa ttgccagcgt ccctgcgcat cctgctgcgt ggctccccgc tgattgagat
    50341 gaaaagacgc atcctggcgg agtgacgttt ctagtgcgtt taacgcggta atgcttgttg
    50401 tcagcccctt gatctccagc gttccctgct gccagcttat cgttgtcagc caggcgtgct
    50461 cgggtaaaag cgccgccagt gcttccagcg cagattgcca gtcgcgggta aattgtcgct
    50521 ggcgctgccg ttgcgaacgc tgttcgcgta actgctgttg ctccagcaaa cgtggcttcg
    50581 ttatctgtaa gctgcgggcg agttgttgtt ccgcctgaag caaaactgcg tctatgcgcg
    50641 cttcggcgct gcctgtcaga cgcagtatta gcgttatccc gacggccagc agcagaggcg
    50701 caacgaacat cagcaaccag aaacgcagaa aagcggtccg gcgttgctgt cgccagggca
    50761 aaaaattaat tggcgggttc atcagtacgc cttcccaagc gccagcccca gcgcgatggc
    50821 aaagtctcca ccaggcggcg gtagcggcgg ctgacgaaca gaaacggcct cccaggggtc
    50881 aaatccgcct tcgccacata tcgcgacgct gtcgggatca acggataacg ctgccgccag
    50941 ctccttcgcg ctagtcatcc ccaccgccag tttgcgcccc cagctatagc gcgtcgccca
    51001 cagccactgt tcgttatcac gccaggccag acattgctga tgagaaggta aaaaaggcag
    51061 gaatcgctgt aatgcactgg catccggggt gatcgcacta acatgaacac gcaacctttc
    51121 tgccagcgta agcagcgttg ccagctcttt gctttgcgcg gcagtcacgt tataagcggg
    51181 gctgagtgag tcttcgctgt aatcgaagcg cagggagtcc ggatccatat ccagctcgcg
    51241 ggccatcgtc cctgacagcc aggccgtttg ctcccgctca ccaagggaca tcgacgggcg
    51301 cggaaatgac cgctgtaatg tgcgactggc gggaaacgcc aacataatgt gatgacgctg
    51361 cggcagttcg cgactccacg gtaacaacgt tttagccagc tgctgcgcat caacaatccg
    51421 cccatctttg ataatgtcgt gcgccagcgg caaccgccac cagcgttgca aaaagcattc
    51481 ttttgcgccc cgcacgatcg caaccgctgc cgcttcttgc tgttgtaaat gcaaaccaat
    51541 ttgccagatc ttaaatgtca ttgtgatgat ctccttatca cccgtcactc tgacgggtat
    51601 atcaatgcgt ctggcttgcc tttatactac cgcgcgtttg tttataaact gcccaaatga
    51661 aactaaatgg gaaatttcca gtgaagttcg taaagtattt tttgatcctt gcagtctgtt
    51721 gcattctgct gggagcaggc tcgatttatg gcctataccg ctacatcgag ccacaactgc
    51781 cggatgtggt gacattaaaa gatgttcgcc tgcaaattcc gatgcagatt tacagcgccg
    51841 atggcgagct gattgctcaa tacggtgaga aacgtcgtat tccggttacg ttggatcaaa
    51901 tcccaccgga gatggtgaaa gcctttatcg cgacggaaga cagccgcttc tacgagcatc
    51961 acggagttga cccggtgggg atcttccgtg cggcaagtgt ggcgctgttc tccggccacg
    52021 cgtcacaagg ggcgagtacc attacccagc agctggcgag aaatttcttc ctcagtccag
    52081 aacgcacgat gatgcgtaag attaaggaag tcttcctcgc gattcgcatt gaacagttgc
    52141 tgacgaaaga cgaaatcctc gagctttatc tgaacaagat ttaccttggt taccgcgcct
    52201 atggtgtcgg tgctgcggca caagtctatt tcggtaaaac ggtcgaccaa ctgacgctga
    52261 acgaaatggc ggtgatagcc gggttgccga aagcgccttc cacctttaat ccgctctact
    52321 caatggatcg tgccgtcgcg cggcgtaacg tcgtactgtc gcggatgctg gatgaagggt
    52381 atatcaccca gcaacagttc gatcagacac gcactgaggc gattaacgct aattatcacg
    52441 cgccggagat tgctttctct gcgccgtacc tgagcgaaat ggtgcgccag gagatgtata
    52501 accgttatgg cgaaagtgcc tatgaagacg gttatcgcat ttacaccacc atcacccgca
    52561 aagtgcagca ggccgcgcag caggcggtac gtaataacgt gctggactac gacatgcgcc
    52621 acggctatcg cggcccggca aatgtgctgt ggaaagtggg cgagtcggcg tgggataaca
    52681 acaagattac cgatacgctg aaggcgctgc cgacctatgg tccgctgctg cctgccgcag
    52741 tcaccagcgc caatcctcag ggagcgacgg cgatgctggc ggacgggtcg accgtcgcat
    52801 tgagtatgga aggcgttcgc tgggcgcgtc cttaccgttc ggatactcag cagggaccga
    52861 cgccgcgtaa agtgaccgat gttctgcaaa cgggtcagca aatctgggtt cgtcaggttg
    52921 gcgatgcatg gtggctggca caagtgccgg aagtgaactc ggcgctggtg tcgatcaatc
    52981 cgcaaaatgg tgccgttatg gcgctggtcg gtggctttga tttcaatcag agcaagttta
    53041 accgcgccac ccaggcactg cgtcaggtgg gttccaacat caaaccgttc ctctacaccg
    53101 cagcgatgga taaaggtctg acgctggcaa gtatgttgaa cgatgtgcca atttctcgct
    53161 gggatgcagg tgccggttct gactggcagc cgaagaactc accaccgcag tatgctggtc
    53221 caattcgctt acgtcagggg ctgggtcagt cgaaaaacgt ggtgatggta cgtgcaatgc
    53281 gggcgatggg cgtcgactac gctgcagaat atctgcaacg cttcggcttc ccggcgcaaa
    53341 acattgtcca caccgaatcg ctggcgctgg gttcagcgtc cttcacccca atgcaggtgg
    53401 cgcgcggcta cgcggtcatg gcgaacggcg gcttcctagt ggacccgtgg tttatcagca
    53461 aaattgaaaa cgatcagggc ggcgtgattt tcgaagcgaa accgaaagta gcctgcccgg
    53521 aatgcgatat tccagtgatt tacggtgata cgcagaaatc gaacgtgctg gaaaataacg
    53581 atgttgaaga tgtcgctatc tcccgcgagc agcagaatgt ttctgtacca atgccgcagc
    53641 tggagcaggc aaatcaggcg ttagtggcga agactggcgc gcaggagtac gcaccgcacg
    53701 tcatcaacac tccgctggca ttcctgatta agagtgcttt gaacaccaat atctttggtg
    53761 aaccaggctg gcagggtacc ggctggcgtg caggtcgtga tttgcagcgt cgcgatatcg
    53821 gcgggaaaac cggtaccact aacagttcga aagatgcgtg gttctcgggt tacggtccgg
    53881 gcgttgtgac ctcggtctgg attggctttg atgatcaccg tcgtaatctc ggtcatacaa
    53941 cggcttccgg agcgattaaa gatcagatct caggttacga aggcggtgcc aagagtgccc
    54001 agcctgcatg ggacgcttat atgaaagccg ttcttgaagg tgtgccggag cagccgctga
    54061 cgccgcctcc gggtattgtg acggtgaata tcgatcgcag caccgggcag ttagctaatg
    54121 gtggcaacag ccgcgaagag tatttcatcg aaggtacgca gccgacacaa caggcggtgc
    54181 acgaggtggg aacgaccatt atcgataatg gtgaggcaca ggaattgttc tgattaaaaa
    54241 ggcgcttcgg cgccttttca gttggctgac aaagtgcatt tgtttatgcc ggatgcgggt
    54301 gaacgcgtta tccggctaac aaaatcgtga aaaatcaata tattgcagga accccataag
    54361 cctgataagc gttgtgcatc aggcaaactt cacgcattta cactcgcccc tgccctttca
    54421 accattcgcg cacgaggaac agcgcgctga cattacgcgc ttcgttgaag tcagggtctt
    54481 ccagcaaatc catcatatgc gccagcggcc agcgcacctg tggtagcggc tctggctcat
    54541 cgccttccag cgattccggg tagagatctt gcgcgaccac gatattcatt ttgctggaaa
    54601 agtaagacgg tgccatgctg agcttcttca aaaaagtcag atcgttcgca ccaaatccaa
    54661 cctcttcttt tagctcgcgg ttagcggctt cgtagacgct ttcacccgga tcaattaatc
    54721 ctttcgaaaa acctaattcg taggattcag ttcccactgc gtattcgcgg atcaggatca
    54781 ggtgatcatc cacaatcggc acaatcatca ctgcttcccg gttggttgga cgcattcgtt
    54841 cataaacacg ccgcacgcca ttgctgaact ccagatccac gctctcgacg gtaaacagtc
    54901 gggaacgggc tacagtttca acattcagaa tggtgggttt ttgtaatgat ttgttcatcg
    54961 tgggatctat gctgtgaaat cagcggttat tgtgcgatat cggacacgct ttcggcaatg
    55021 tgaattgcat gttatttaca tttatgtaac ttaataaata atcgtcctca aatcaaatta
    55081 aaagtcaata ggttgaaata actccaggaa tttgctgata ttccgccttc agagggtttg
    55141 ctatgatcag cggttactgt gatgtgctta atgatgctca agttaagctc cacgcttgcc
    55201 gatagccaac cgcagaatca tgtattgtgt ccggtgcgac tgaccacgcc tgacagacta
    55261 agtaagatgg ggaaagcatg agcaccattg tgattttttt agctgctttg ctggcctgct
    55321 cactacttgc gggatggctg ataaaagtgc gatccagacg gcgtcagctg ccctggacca
    55381 acgccttcgc ggatgcgcaa acgcgtaaac tcacacctga agaacgtagc gccgttgaaa
    55441 attatcttga gagcctgacg caggtattac aggtgcctgg cccaacggga gctagcgcgg
    55501 caccgatctc tctggcgctg aatgccgaaa gcaacaacgt catgatgctg acacacgcta
    55561 tcacacgtta cggcatctct accgacgatc cgaataaatg gcgttactac ctcgattcgg
    55621 tagaagtcca cctgcccccc ttctgggaac agtacatcaa cgatgagaat accgttgaac
    55681 tgattcatac cgattcgctg ccgttggtta tttcactcaa cggtcatacg ctgcaggagt
    55741 acatgcagga aacgcgcggc tatgccttgc aacctgttcc gtccacgcag gcgtcgattc
    55801 gcggggaaga gagtgagcaa atcgagctac tcaatattcg caaagaaacg catgaagaat
    55861 atgcgctgag tcgtccgcgc gggctgcgtg aagcgttgct gatcgtcgcc tccttcctga
    55921 tgttcttttt ctgcctgatt accccggatg tatttgttcc gtggctggca ggcggcgcat
    55981 tactgctgct gggcgcaggt ctgtgggggc tattcgcgcc cccggcaaaa tcctccctgc
    56041 gagaaattca ttgtctgcgc ggtacacccc gtcgttgggg attgtttggc gaaaacgatc
    56101 aggaacagat caacaatatt tcactcggta ttatcgacct ggtctatccc gcacactggc
    56161 agccatacat tgctcaggat ctcggtcaac aaaccgatat tgatatctat ctcgaccgcc
    56221 acgtagtgcg tcaggggcga tatctttcgc tgcatgatga agtaaaaaac tttccgttac
    56281 agcactggct gcgcagtacg attatcgctg cgggctcgct gctggtgctg tttatgctgt
    56341 tattctggat cccgctggat atgccgctga aattcactct ctcatggatg aaaggcgcgc
    56401 agaccattga agccaccagc gtaaaacaac tggctgacgc tggcgtgcgg gtaggcgata
    56461 cattgcgtat tagcggtacg ggaatgtgta atattcgcac ttccggaacc tggagcgcga
    56521 aaaccaattc acctttttta ccgtttgact gctcgcagat catctggaat gacgcccgct
    56581 cattaccatt accagaatct gaactggtca acaaagcgac ggcattgact gaagcagtta
    56641 atcgccagct gcaccctaaa ccggaagatg aatctcgcgt cagtgcctca ttgcgttcag
    56701 cgattcaaaa atccggcatg gtattgcttg atgattttgg cgacattgta ctgaagacag
    56761 cggatttatg ttctgccaaa gatgactgtg tgcgactgaa aaatgcgctg gtcaatctcg
    56821 gcaacagtaa agactgggac gcgctggtaa aacgcgccaa cgccgggaag ctcgatggcg
    56881 tgaatgtgtt attacgcccg gtgagtgcgg aatcgctgga taacctggtg gcaacctcca
    56941 ccgcgccgtt tatcacgcat gaaacggcgc gagcggcaca atcactaaac agtccggccc
    57001 ccggcggatt cctgattgtc agcgacgaag gcagcgattt tgttgatcag ccctggcctt
    57061 cggcatcact ttacgactac ccgccgcaag aacagtggaa cgctttccag aaactggcac
    57121 aaatgctgat gcatacgccg tttaacgccg aaggtatcgt cacaaaaatc ttcactgacg
    57181 ccaatggtac gcagcatatt ggccttcatc cgatcccgga tcgttccggc ctgtggcgct
    57241 atctcagcac cacattgctg ctactgacga tgctgggtag cgccatttac aatggcgtac
    57301 aggcctggcg tcgttaccag cgtcatcgca ctcgcatgat ggagattcag gcctattatg
    57361 aaagctgcct gaacccgcaa ctgatcaccc cttcagaaag ccttatcgaa taacacgttt
    57421 gcgcggcagg ttatgctacc ctgtcgcgca aattgcttca ctctggagat ttccctcatg
    57481 catatcaaca ttgcctggca ggacgtagat accgttctgc tggatatgga cggcacgttg
    57541 ctcgacctcg ccttcgataa ctatttctgg caaaagctgg tgcctgaaac atggggcgcg
    57601 aaaaacgggg ttacgccaca ggaagcgatg gaatatatgc gccagcaata tcacgacgta
    57661 cagcatacgc taaactggta ctgtcttgat tactggagtg agcaactggg tctggatatc
    57721 tgtgcgatga ccaccgagat gggaccgcgc gccgtactgc gtgaagatac cattccgttt
    57781 cttgaggcac tgaaagccag cggtaagcag cgaattttgc tcaccaatgc tcatccgcac
    57841 aacctggcgg taaaacttga gcatacgggt ctggacgcac accttgattt attactttcc
    57901 acccacacat ttggttatcc gaaagaggat cagcggttat ggcatgcggt ggccgaagct
    57961 acgggtctga aagctgaaag aacgctgttt attgatgaca gcgaagcgat tctcgatgct
    58021 gccgcgcaat ttggtattcg ttactgcctc ggcgtgacta atcctgattc cgggattgcc
    58081 gagaaacagt atcaacgcca tccgtcactg aatgactacc gccgcctgat cccctcgcta
    58141 atgtgaagga gacgccatga aagagaaacc tgctgttgag gttcgactgg ataaatggct
    58201 atgggctgcc cgtttttata aaacccgcgc gctggcccgt gaaatgattg aaggcggtaa
    58261 ggtgcattac aacgggcagc gcagcaagcc gagcaaaatc gtcgagctga atgccacgct
    58321 cactctgcgc cagggaaatg acgaacgcac ggtgattgta aaggcgatta ctgaacagcg
    58381 tcgccccgcc agcgaggcag ccttgctgta tgaagagact gcggaaagtg tagagaaacg
    58441 cgaaaaaatg gcgatggcac gtaaacttaa tgccttaacc atgccgcacc cggaccgacg
    58501 cccggacaaa aaagagcgcc gcgacctgtt acgatttaaa cacggcgaca gtgaataact
    58561 gtcacctgca agagagatga ttatgccgca acatgaccaa ttacatcgct atctgtttga
    58621 aaattttgcc gttcgcggcg aactggtaac cgtttcggaa accctgcaac agatccttga
    58681 gaaccacgat tatccgcagc ccgttaaaaa cgtgctggca gaactgctgg ttgcgaccag
    58741 cctgttaacc gctacgctga agtttgatgg tgatatcacc gtacagctgc agggcgacgg
    58801 tccgatgaat ctggcggtta ttaacggtaa caataaccag caaatgcgcg gtgtggcgcg
    58861 cgtgcagggc gaaattccag aaaatgccga cctgaaaacg ctggtcggca atggttacgt
    58921 ggtgatcacc attaccccga gcgaaggcga acgctatcag ggcgtagttg gtctggaagg
    58981 tgataccctg gcggcctgcc tagaagatta ctttatgcgt tctgaacagc tgccgacgcg
    59041 cctgtttatt cgcaccggcg acgtagacgg caaaccggct gcaggcggta tgttgttgca
    59101 ggtaatgcct gcgcaaaata cccaacagga cgactttgac cacctggcga cgctaaccga
    59161 aaccatcaaa accgaagaac tgctgacctt accggcaaac gaagtgttgt ggcgtttgta
    59221 tcacgaagaa gaggtgacgg tttacgatcc gcaggatgtg gagttcaaat gcacctgctc
    59281 gcgtgaacgt tgcgccgatg cgctgaaaac gctgcctgat gaagaagttg atagcatcct
    59341 ggcggaagat ggcgaaattg acatgcattg tgattactgc ggtaaccact atctgttcaa
    59401 tgcgatggat attgctgaaa tccgcaacaa cgcgtctccg gcagatccgt aagttcatta
    59461 atgagtatgt ccggcagaga gggctctgcc ggattttacg acttaatggc ctgatgtgct
    59521 gcgcttacca gcgcctatga aataaatcgc aaattatttt ggtcagatac agcgtcgtat
    59581 ccgataaaag cgttgtcact tacttaagtt tatttccagc gtttcaccat ctaccgtcag
    59641 atcgcgccac gctttcggcc tcgtccccag ttttccatcc ttcgcagctg tgagtaattt
    59701 ttctttcgtt atttcgttcg gaagtaaact catatcaagc gcgtcccatt cttttctgtc
    59761 tgagtcaaag ccatagacaa tgactctgtc atcattaaaa gcaaacagga tccgctccgc
    59821 ctgaccatca ctattcagat cttgctcgac cagtacgcag gcgtcttttt caatacaggt
    59881 catcacgtta tagcgatcct ggattaaggc cgaccagaat gtcgcatccg gtttaacaga
    59941 accaggggca attaacacat tttcggcgaa tactttttcc gatacctgtt cctgaagatg
    60001 ttgctctcca tctaacgcca tcagcagatc ccgcgcgcgt ttcgggtctt tcataaaccc
    60061 ggcatcgctt ttcagcgact caagcgccgc acgtccatag cgaccgctct gttcgagcat
    60121 gtagatagtt acctggtctg gcgtgttttt gccgctctga taacgcgcca tatgactgtt
    60181 cacgctaata cgcatactgt ccagcaccgg cgaattaaga agcaccagta tcaccaacac
    60241 caataacgaa accgcaaggt tcactttgcc ctgaagaaca aggggatttt gcccattacg
    60301 ccagacgata ctgacaaaat accccagcga ccacaccagt aacaccagct ccgccagcgc
    60361 gccctgcaag cggtcgacag tccagccgta ctgagcgacc cgcagccata acccccaggt
    60421 ggcgacgaac acatacagcg gagcaaccag caaagcggtt ttaatcaggc aacgcaacgg
    60481 ccccgtccag ggaagtgacg ctttttgcgg atcgcggaca atagccatta agatcaattg
    60541 caaaaaggcc agcgttaaca gcaacccggc ggcggagatg tggcgagaaa tcgcgctcag
    60601 gcccgtaaac ggcagggtga tgataaacat cggggttagc aatgatacta acggcagcaa
    60661 ccccgtggcg attagcgtga acaacttttg aatagagtcg attaaacgtg actgtgtacg
    60721 cgcgaggatc accgccagtg cggtaaccag accaaatgta agatacataa accagtcggt
    60781 tgcaaaaaag agcgtattga aaaacgtgat gccaacaagt ttaaacaact caccccacag
    60841 taaaagcacc agccacgtca ggccattgga gagaaaaatc accagtaata taagtacgtt
    60901 atgccatact gactggtaaa agtagctata gcggctactg ccattgcgga tacgaaggct
    60961 ttgttgtatc cacggtagca acaacattgc catcaacagc agatagcagc caaaatccca
    61021 aatcgccttt tcagctctcc aggggttcat gccatcagtt tgccacttca gccaaccgct
    61081 catccctgtc gtggcaataa acaccagcgc cagccatccc caaaggcgct tctgtttaaa
    61141 agagatcacg gagaagagta aaacggatga aaacgcgacg gtcgcaggca cgccataaac
    61201 aatccaactg tgattttttc cggcaagcca ggcgataagc aggtagcaaa ccagcccctg
    61261 aagtaatccg gtagcaatca tcccccagcg tgttgccggt gaaagttcaa cgttatccat
    61321 gttggttatc cagaatcaaa aggtgggtta attatcgcat ccgggcagta gtattttgct
    61381 tttttcagaa aataatcaaa aaaagttagc gtggtgaatc gatactttac cggttgaatt
    61441 tgcatcaatt tcattcagga atgcgattcc actcacaata ttcccgccat ataaaccaag
    61501 atttaacctt ttgagaacat tttccacacc taaaatgcta tttctgcgat aatagcaacc
    61561 gtttcgtgac aggaatcacg gagttttttg tcaaatatga atttctccag atacgtaaat
    61621 ctatgagcct tgtcgcggtt aacaccccca aaaagacttt actattcagg caatacatat
    61681 tggctaagga gcagtgaaat gcgcgttaac aatggtttgc ccccgcaaga actcgaggct
    61741 tatggtatca gtgacgtaca tgatatcgtt tacaacccaa gctacgacct gctgtatcag
    61801 gaagagctcg atccgagcct gacaggttat gagcgcgggg tgttaactaa tctgggtgcc
    61861 gttgccgtcg ataccgggat cttcaccggt cgttcaccaa aagataagta tatcgtccgt
    61921 gacgatacca ctcgcgatac tttctggtgg gcagacaaag gcaaaggtaa gaacgacaac
    61981 aaacctctct ctccggaaac ctggcagcat ctgaaaggcc tggtgaccag gcagctttcc
    62041 ggcaaacgtc tgttcgttgt cgacgctttc tgtggtgcga acccggatac tcgtctttcc
    62101 gtccgtttca tcaccgaagt ggcctggcag gcgcattttg tcaaaaacat gtttattcgc
    62161 ccgagcgatg aagagctggc aggtttcaaa ccagacttta tcgttatgaa cggcgcgaag
    62221 tgcactaacc cgcagtggaa agaacagggt ctgaactccg aaaacttcgt ggcgtttaac
    62281 ctgaccgagc gcatgcagct gattggcggc acctggtacg gcggcgaaat gaagaaaggg
    62341 atgttctcga tgatgaacta cctgctgccg ctgaaaggta tcgcttctat gcactgctcc
    62401 gccaacgttg gcgagaaagg cgatgttgcg gtgttcttcg gcctttccgg caccggtaaa
    62461 accacccttt ccaccgaccc gaaacgccgc ctgattggcg atgacgaaca cggctgggac
    62521 gatgacggcg tgtttaattt cgaaggcggc tgctacgcaa aaactatcaa gctgtcgaaa
    62581 gaagcggaac ctgaaatcta caacgctatc cgtcgtgatg cgctgctgga aaacgtcacc
    62641 gtgcgtgaag atggcactat cgactttgat gatggttcaa aaaccgagaa cacccgcgtt
    62701 tcttatccaa tctatcacat cgataacatc gttaagccgg tttccaaagc gggccacgcg
    62761 actaaggtta tcttcctgac tgctgatgct ttcggcgtgt tgccgccggt ttctcgcctg
    62821 actgccgatc aaacccagta tcacttcctc tctggcttca ccgccaaact ggccggtact
    62881 gagcgtggca tcaccgaacc gacgccaacc ttctccgctt gcttcggcgc ggcgttcctg
    62941 tcgctgcacc cgactcagta cgcagaagtg ctggtgaaac gtatgcaggc ggcgggcgcg
    63001 caggcttatc tggttaacac tggctggaac ggcactggca aacgtatctc gattaaagat
    63061 acccgcgcca ttatcgacgc catcctcaac ggttcgctgg ataatgcaga aaccttcact
    63121 ctgccgatgt ttaacctggc gatcccaacc gaactgccgg gcgtagacac gaagattctc
    63181 gatccgcgta acacctacgc ttctccggaa cagtggcagg aaaaagccga aaccctggcg
    63241 aaactgttta tcgacaactt cgataaatac accgacaccc ctgcgggtgc cgcgctggta
    63301 gcggctggtc cgaaactgta atgatttgaa gctggagaat atctatccag tatcttatag
    63361 aaagcaaaac gggaggcacc ttcgcctccc gtttatttac ccttcttttg tcgtgccctg
    63421 cgcccgcgtt accggcactg gcagccaggc gcgaatggaa agcccgcccc gctcgctggt
    63481 gccaagctcc agcatcccgt tatggttatc cacgatacgc tgcacaattg ccagccctaa
    63541 tcccgtgccg ctaatggtgc gcgcactgtc gccgcggaca aacggctgga acaggtgctt
    63601 acgttgttcc ggcgcaattc ccggaccgtc atcttccacc tggaaccagg cgcgattcgg
    63661 ctccgttccg ctgctgactt tgatccagcc attgccataa cgggcggcgt tgaccaccat
    63721 attcgccacc gcgcgtttga tcgacagcgg gtgcattttc acttcaatgc tgccggggta
    63781 aagcgcggtt tcaatttccc gctcatagcc actttcggca gcaatcacct caccgagcac
    63841 tgcattaaga tccgccattt ccatcggcat ctcctgcccg gtgcgcaggt agtcgataaa
    63901 ctgctcaatg atggcgttgc actcttcgat atctttattg atcgattctg ccagatagcc
    63961 atcctgctcg ctcatcatct cagtcgccag gcgaatacgc gtcagcggcg tgcgcaagtc
    64021 gtgacttacc cccgccatca gcagcgtgcg gtcatccgcc agttgcttaa caccagccgc
    64081 catatggtta aaggcacggg taacggaacg cacctccgaa gcgccatatt cacgcagcgg
    64141 cggcggaata atccctttac caacctgcaa ggctgcgtgt tcgagatcga ccaacggtcg
    64201 gttctggata cgaataaaca gccacgcccc gcctatcgcc aatagcataa tcgccagcgt
    64261 atagcggaac agcggagaga aatcgccctg atgaatttcg gtcagcggca cgcgtaccca
    64321 gatattgggc gacagccagg ttttcagcca gacgacaggc gaacttttgt tgacctcaac
    64381 gcgcacttcc gtcgggccgc ccagttgctg tgccatctga tggcttaaga attcatagtg
    64441 ttgcgcccaa cgcagacctg cctcttcggc agcctcgttg gagtagagag agatccccag
    64501 ctcacggtag atctcccgac ggaaagcggg aggcacaacc aactgcgtgc cgtcctccag
    64561 ttgcagtttg tcggtcatca acatacgcac ttcgtacgcg aggactttat taaactgctg
    64621 gaggctcggc aaaatcgcga agttcagcac caccagataa gtcgtcacca ggctggcgaa
    64681 cagcaaggtg acgatgagca ataacgtacg ggcaaatgaa cttcgtggcg agaagcgcaa
    64741 tcgcctcatg ctttagagcc gtccggtaca aagacgtagc ccaggcccca aacggtctga
    64801 atgtaacgcg gatgcgctgg atcttcttct accatgcggc gcagacgcga aatctgcacg
    64861 tcgatggagc gttccattgc ggaatattca cgaccacggg caaggttcat cagcttatcg
    64921 cgggagagcg gctcacgcgg atggctgacc agtgccttca gtaccgcaaa ctcaccgctg
    64981 gtgagcggca tcggctcatc ttcgcggaac atttcgcgcg taccgaggtt aagtttgaac
    65041 ttaccgaaag caattaccgc ctcttcctgt gacggtgcgc ctggcagttc gttcgcctga
    65101 cgacgcagca ccgcacgaat acgggccagc agttcacgcg ggttaaacgg ttttggaatg
    65161 tagtcgtcag cgccaatctc caggcctacg atacggtcca cttcttcccc tttcgccgtc
    65221 accataatga tcggcatcgg gttgctctga ctacgaagac gtcggcaaat cgacaagcca
    65281 tcttcaccag gtaacattaa atccagtacc ataagatgga aagattcacg agtcagcagg
    65341 cgatccatct gttctgcatt agcgacgctt cgaacctgga agccttgttc ggtgagataa
    65401 cgttccagca gcgcacgcag gcgcatgtcg tcatcgacca ccagaatctt gtagttctct
    65461 tgcattgttt gtactcccaa aggttcgcaa caatttgtaa gcgtgtattc ttaaaaaagc
    65521 tcacgttcgt caccagctaa atctggtata aatttcagcc taaattgtta caaagcatat
    65581 taaacagcag cttaagtata caatttattc ggcgaaacat tattgattct gttgatatga
    65641 tcacgttata cccaatgtgc gcattatcaa acagacaaag ggaatcaacg agatgaaaac
    65701 gcccctggtt acccgggaag ggtatgaaaa actcaaacaa gagcttaatt atctctggcg
    65761 tgaagaacgc ccggaggtca caaaaaaggt gacctgggcc gcaagtctgg gcgaccgcag
    65821 cgaaaatgct gactatcagt ataataaaaa gcgtctgcgt gaaatcgacc gtcgcgtgcg
    65881 ctatctcact aaatgcctgg aaaatctcaa aatcgtcgat tactcccctc agcaggaagg
    65941 caaagtattt tttggcgcgt gggtggagat tgaaaacgac gatggcgtga ctcaccgttt
    66001 ccgtattgtc ggctacgatg aaatttttgg ccgtaaagat tacatctcta tcgattcccc
    66061 gatggcccgc gcattgctga aaaaagaagt cggcgatctg gcggtggtga atacccctgc
    66121 cggggaagcg aactggtatg ttaatgctat cgagtacgtg aaaccgtaag gaagagtctt
    66181 aacctcctgc cgatggctgg catttttgcc agccagtccg tataactatc ccctgatttt
    66241 tgatccgaaa agatgaactc aaaccatgat gaatgattcg ttctgccgca ttattgcggg
    66301 tgaaattcag gcgcgcccgg aacaggttga cgctgccgtt cgcctgcttg acgaagggaa
    66361 taccgtgccg tttatcgcac gttatcgtaa ggaaatcacc ggcggtctgg atgacacgca
    66421 gctgcgtaat ctggaaacgc gtctgagcta tctgcgcgag ctggaagaga gacgtcaggc
    66481 gatcctcaaa tccatttccg agcaaggcaa actcaccgat gatctggcga aggccatcaa
    66541 cgccacccta agcaaaaccg aactcgaaga cctctacctg ccctacaaac ctaaacgccg
    66601 cacccgcggg caaatcgcca ttgaagcagg gcttgagccg ttggctgacc tgctgtggag
    66661 cgatccgtca cacacgccag aagtcgccgc tgcacaatat gttgatgccg ataaaggcgt
    66721 ggcagatacc aaagccgcgc tggatggcgc gcgctatatc ctgatggaac ggtttgccga
    66781 agatgccgcg ctgctggcga aagtgcgtga ttatctgtgg aagaacgcgc atttggtttc
    66841 tacggtggtg agcggtaaag aagaggaagg ggcgaaattc cgcgactatt tcgatcatca
    66901 cgaaccgttg tccacggtgc cttctcaccg cgcgctggcg atgttccgtg ggcgtaacga
    66961 aggcgtactc cagctttcgc tgaatgccga tccgcagttc gatgagccgc ccaaagagag
    67021 ctattgcgag caaatcatca tggatcacct tggcctgcgc ctgaacaatg ccccggcgga
    67081 tagctggcgc aaaagcgtag tgagctggac ctggcgcatc aaggtgctga tgcatctgga
    67141 aaccgaactg atgggtaccg tgcgcgaacg cgcggaagat gaagcaatca acgtctttgc
    67201 ccgtaacctg cacgatctgc tgatggcggc ccctgccgga ctgcgtgcaa cgatgggcct
    67261 cgatccgggt ctgcgtaccg gggtaaaagt ggcggtggtc gatgccactg gcaaactggt
    67321 ggcgaccgat accatttacc cgcacaccgg acaagccgca aaagcagcga tgaccgttgc
    67381 tgccttgtgt gaaaaacata acgttgaact ggtggcgatc ggcaacggta cagcttcccg
    67441 cgaaactgaa cgtttctatc tcgacgtgca gaagcagttc ccgaaagtga ccgcacagaa
    67501 agtgatcgtc agcgaagctg gcgcgtcggt ttactcagct tccgagctgg cagcgcagga
    67561 gttcccggat ctcgacgttt cgctgcgtgg tgcggtatct atcgcccgcc gtttgcagga
    67621 tccgctggcg gagctggtga aaatcgatcc gaaatctatc ggcgtaggtc agtatcagca
    67681 tgacgtcagc cagacgcaac tggcccgcaa actggatgca gtagtagaag actgcgtaaa
    67741 cgccgttggc gtcgatctca acaccgcttc tgttccgcta ttaacccgcg tggcgggcct
    67801 gacgcgcatg atggcgcaaa acatcgtggc ctggcgcgat gagaacggcc agttccagaa
    67861 ccgtcagcaa ctgctgaaag tcagccgtct ggggccgaaa gccttcgagc agtgcgcggg
    67921 cttcctgcgc attaaccacg gtgataaccc gctggacgcc tctaccgttc atccggaagc
    67981 ctatccggtg gtggaacgca ttctggcagc aacacagcag gcactgaaag atctgatggg
    68041 taacagcagc gaactgcgta acctgaaagc gtctgacttt actgatgaaa aattcggtgt
    68101 gccgacagta actgacatca tcaaagagct ggaaaaaccg ggtcgcgatc cgcgtccgga
    68161 atttaaaacc gctcagtttg ccgatggcgt cgagacaatg aacgacctgc aaccgggtat
    68221 gatcctcgaa ggcgcagtga ccaacgtcac caactttggc gcgtttgtcg atattggcgt
    68281 gcatcaggac ggcctggttc acatctcttc attatcgaac aagtttgtgg aagatccgca
    68341 taccgtggtg aaagcgggcg acattgtgaa ggtgaaagtg ctggaagtgg atcttcagcg
    68401 taaacgtatc gccctgacta tgcgtctgga cgagcagcct ggcgaaacca actctcgtcg
    68461 cggcggcggt aatgaacgcc cgcaaaacaa ccgcccggca gccaaaccac gcggtcgtga
    68521 agcgcagcct gccggtaata gcgcgatgat ggatgcgctg gcggcagcga tgggtaaaaa
    68581 acgttaaacg cccggactgg cccctaaacc tgcgcgataa ggcgttcacg ccgcatctgg
    68641 caacgaacgc tttgcctgac ataaaagtgc cggagaatat ctccggcact tttattccac
    68701 agccaaactc acaatatact ccgttaatat ttatcacttc attaacaact gaaaccttaa
    68761 ttaaacatta gccagtccgg gtaactcact attcgaatta tattttcgct gcgatataac
    68821 cttgagccac atcaacattg agtcagatta ttattcaaac caacattcgc acacatttta
    68881 agtattgctg atagaaacca ttctcattat cattgtgttg ttgattattt aatctctcct
    68941 ttgttggcaa atcatctggt ctcatgtcgc ggtcaaacgc cccatgaggt agttatccag
    69001 ttaatgagaa acaagtaggc acctatgcaa tacactccag atactgcgtg gaaaatcact
    69061 ggcttttccc gtgaaatcag cccggcatat cgccaaaaac tgctttctct aggcatgtta
    69121 cctggctcct cttttgatgt ggtgcgcgtc gctccactcg gcgaccccat tcatatcgaa
    69181 acccgtcgtg tgagcctggt attacgcaaa aaagatctgg ccttattaga agtggaagcg
    69241 gtttcctgtt aatacggtga taacaacaat gaaaaaatta accattggct taattggtaa
    69301 tccaaattct ggcaagacaa ccttatttaa ccagctcact ggcgcacgtc agcgtgtagg
    69361 taactgggct ggcgttaccg tcgaacgtaa agaagggcaa ttctccacca ccgatcatca
    69421 ggtcacgctg gtggacctgc ccggcaccta ttctctgacc accatctcat cgcagacctc
    69481 gctcgatgag caaatcgcct gtcactacat tttgagtggc gacgccgacc tgctgattaa
    69541 cgtggtggat gcgtctaacc ttgagcgtaa cctgtacctg acgctgcaac tgctggaact
    69601 cggcattccc tgcatcgtgg cgctgaacat gctcgacatt gccgagaagc aaaatattcg
    69661 tattgaaatt gatgctctat cggcacgtct tggctgcccg gtgatcccgc tggtttcaac
    69721 ccgtggtcgc ggtattgaag cgctcaagct ggcgattgat cgctataaag ctaacgagaa
    69781 tgtggaactg gtgcattacg cacagccgct actcaacgaa gcagattcac tggcaaaagt
    69841 gatgccttcc gacatcccgc tgaaacaacg tcgctggctg ggcctgcaaa tgctggaagg
    69901 cgatatctac agccgcgcct acgccggtga agcgtcgcag catctggatg ccaccctcgc
    69961 ccgtctgcgt aatgaaatgg atgatccggc gctgcacatt gccgatgcgc gttaccagtg
    70021 cattgctgcc atctgtgatg tggtaagcaa caccctgacg gcagaaccca gccgtttcac
    70081 cactgcggta gataaaatcg tgctcaaccg tttcctcggt ctgccgattt tcctctttgt
    70141 gatgtacctg atgttcctgc tggctatcaa catcggcggg gcgttacagc cgctgtttaa
    70201 cgtcggctcc gtggcgctat ttgtgcatgg tattcaatgg attggctaca cgctccactt
    70261 cccggactgg ctgactatct tcctcgccca gggcctgggt ggcggtatta acaccgtgct
    70321 gccactggtg ccgcagatcg gcatgatgta cctgttcctc tccttccttg aggactccgg
    70381 ctatatggcg cgtgcagcgt ttgtgatgga ccgcctgatg caggcgctgg gcttgccggg
    70441 gaaatccttt gtgccgctga tcgtcggttt cggttgtaac gtaccgtcgg taatgggcgc
    70501 acgtacgctt gatgcaccgc gtgaacgtct gatgaccatc atgatggcac cgtttatgtc
    70561 ctgcggcgcg cgtctggcta tcttcgcagt attcgcggct gccttcttcg ggcagaacgg
    70621 tgccctggcg gttttctcgc tgtatatgct cggtatcgtg atggcggtgc tgactggcct
    70681 gatgctcaag tacaccatca tgcgcggtga agcgacgccg tttgtcatgg agctgccggt
    70741 ctaccatgta ccacacgtta aaagcctgat tatccagacc tggcagcgtc tgaagggctt
    70801 cgttctgcgt gctggtaaag tgattatcat cgtcagcatt ttcctgagcg ctttcaacag
    70861 cttctcgttg agcgggaaaa tcgtcgataa catcaacgac tcggcgctgg cttccgtcag
    70921 ccgggtgata acccctgtct tcaagccgat tggcgtacat gaagataact ggcaggcaac
    70981 ggttggcctg tttacgggcg caatggcaaa agaagtggtg gtgggtacgc tcaacaccct
    71041 ctacaccgca gaaaatattc aggacgaaga gttcaatccg gcggaattca acctcggtga
    71101 agagctgttc agtgcggtag atgaaacctg gcagagcctg aaagacacct tcagccttag
    71161 cgtactgatg aaccccattg aagctagcaa aggcaacggt gaaatgggta ccggggcgat
    71221 gggcgtgatg gatcagaaat tcggtagcgc agctgccgca tacagctacc tgattttcgt
    71281 cctgctgtat gtaccatgta tctcggtgat gggagccatc gcccgtgaat caagccgtgg
    71341 ctggatgggc ttctccatcc tgtgggggct gaatatcgct tactcactgg caacattgtt
    71401 ctatcaggtc gccagctaca gtcagcaccc aacttacagc ctggtgtgca ttctggcggt
    71461 aatcctgttt aacatcgtag ctatcggtct gctgcgccgc gcgcgtagcc gggtggatat
    71521 cgaactgctg gcaacccgca agtcggtaag cagttgctgc gcagccagca ccaccggtga
    71581 ttgccattaa tggcttcact tattcaggta cgtgatttgc tggcgttacg gggccgtatg
    71641 gaagcgaccc agataagcca gacattgaac actccgcagc caatgattaa cgccatgctg
    71701 caacagctgg aaagcatggg caaagccgtg cggattcagg aagaacctga cggctgcctc
    71761 tctggcagtt gtaaaagctg cccggaaggc aaagcctgtc tgcgcgagtg gtgggcgctg
    71821 cgttaaccat actccatcgc ctgttttgaa aagcccggta tgcgtttgca tccgggcttt
    71881 tttgcgtgcg gctttccata aaaatgcaat tcttgcagcg caacgtaagt tcctctgaaa
    71941 gcgtctcgca gggatgaaaa ctcgctaatg cacagatgtg gagtggcgcg tagagtcgcg
    72001 gcattcaaac aacaggtgaa ggaacgccat gagtaaaaag cagagttcca ccccacatga
    72061 tgcgctgttc aaactctttt tacgccaacc ggagacggca cgcgattttc ttgcgtttca
    72121 tttaccggca cccattcacg cgctctgtga tatgaaaacc ctcaagctgg agtcgaggag
    72181 ctttattgat gacgatctgc gtgaaagcta ttccgatgtg ctgtggtcgg tgaaaacgga
    72241 acaaggacca ggatacatct attgtctgat tgaacatcaa agcacctcaa acaaactgat
    72301 cgcatttcgc atgatgcgtt acgctattgc cgcaatgcaa aatcaccttg atgctggata
    72361 caaaacgttg ccgatggtgg tgccattgtt gttttaccac ggtattgaaa gcccctatcc
    72421 ctattcgctg tgttggctgg attgtttcgc cgatcccaaa ctggcaaggc agctttatgc
    72481 ctccgcattt ccgctgattg atgtcaccgt catgcctgat gatgaaatca tgcagcaccg
    72541 acgtatggcg ctgctggagt taattcaaaa acatattcgt caacgcgatc tgatggggct
    72601 ggtagagcaa atggcctgct tattaagtag tggatacgct aatgacagac aaatcaaagg
    72661 gctgtttaat tacatactgc aaactggcga cgctgtacgt tttaacgatt ttatcgacgg
    72721 cgttgccgaa cgttcaccga aacacaagga gagtttaatg actattgcgg aaagattgcg
    72781 gcaggagggg gaacaatcca aagccctgca tatagccaaa ataatgcttg aatccggagt
    72841 ccctcttgca gacatcatgc gatttaccgg gctgtcagaa gaagagttgg ctgcggcgag
    72901 tagactggcc ccctgaatct ccagacaacc agtat
//
LOCUS       DAEEGA010000006        70704 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEEGA010000006 DAEEGA010000000
VERSION     DAEEGA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70704
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.006"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..794
                     /locus_tag="KLH46_000396"
     CDS             <1..794
                     /locus_tag="KLH46_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE0377219.1"
                     /translation="LPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDD
                     DNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFA
                     LIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQF
                     VVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYL
                     SGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            805..1896
                     /locus_tag="KLH46_000397"
     CDS             805..1896
                     /locus_tag="KLH46_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0377220.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTAAITVMVR"
     gene            complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="KLH46_000398"
     CDS             complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="KLH46_000398"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBE0377221.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2864..4900
                     /gene="lpoA"
                     /locus_tag="KLH46_000399"
     CDS             2864..4900
                     /gene="lpoA"
                     /locus_tag="KLH46_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBE0377222.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4858..5253
                     /locus_tag="KLH46_000400"
     CDS             4858..5253
                     /locus_tag="KLH46_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBE0377223.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5273..5863
                     /gene="diaA"
                     /locus_tag="KLH46_000401"
     CDS             5273..5863
                     /gene="diaA"
                     /locus_tag="KLH46_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBE0377224.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5873..6448
                     /gene="yraP"
                     /locus_tag="KLH46_000402"
     CDS             5873..6448
                     /gene="yraP"
                     /locus_tag="KLH46_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBE0377225.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6562..7602)
                     /locus_tag="KLH46_000403"
     CDS             complement(6562..7602)
                     /locus_tag="KLH46_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBE0377226.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETLQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVCILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7675..8310)
                     /locus_tag="KLH46_000404"
     CDS             complement(7675..8310)
                     /locus_tag="KLH46_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBE0377227.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8438..8956
                     /gene="yhbO"
                     /locus_tag="KLH46_000405"
     CDS             8438..8956
                     /gene="yhbO"
                     /locus_tag="KLH46_000405"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBE0377228.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8936..9379)
                     /locus_tag="KLH46_000406"
     CDS             complement(8936..9379)
                     /locus_tag="KLH46_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBE0377229.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9430..9732
                     /gene="yhbQ"
                     /locus_tag="KLH46_000407"
     CDS             9430..9732
                     /gene="yhbQ"
                     /locus_tag="KLH46_000407"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBE0377230.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9719..10222)
                     /locus_tag="KLH46_000408"
     CDS             complement(9719..10222)
                     /locus_tag="KLH46_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE0377231.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10216..10740)
                     /locus_tag="KLH46_000409"
     CDS             complement(10216..10740)
                     /locus_tag="KLH46_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBE0377232.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10949..11944
                     /locus_tag="KLH46_000410"
     CDS             10949..11944
                     /locus_tag="KLH46_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBE0377233.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11953..12831
                     /locus_tag="KLH46_000411"
     CDS             11953..12831
                     /locus_tag="KLH46_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBE0377234.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12912..13919
                     /locus_tag="KLH46_000412"
     CDS             12912..13919
                     /locus_tag="KLH46_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBE0377235.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="KLH46_000413"
     CDS             complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="KLH46_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBE0377236.1"
                     /translation="MATRTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="KLH46_000414"
     CDS             complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="KLH46_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBE0377237.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="KLH46_000415"
     CDS             complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="KLH46_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBE0377238.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="KLH46_000416"
     CDS             complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="KLH46_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBE0377239.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="KLH46_000417"
     CDS             complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="KLH46_000417"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBE0377240.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRAMTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="KLH46_000418"
     CDS             complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="KLH46_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBE0377241.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21297..22241)
                     /gene="truB"
                     /locus_tag="KLH46_000419"
     CDS             complement(21297..22241)
                     /gene="truB"
                     /locus_tag="KLH46_000419"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBE0377242.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="KLH46_000420"
     CDS             complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="KLH46_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBE0377243.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22806..25454)
                     /gene="infB"
                     /locus_tag="KLH46_000421"
     CDS             complement(22806..25454)
                     /gene="infB"
                     /locus_tag="KLH46_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBE0377244.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="KLH46_000422"
     CDS             complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="KLH46_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBE0377245.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="KLH46_000423"
     CDS             complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="KLH46_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBE0377246.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27653..27729)
                     /locus_tag="KLH46_000424"
     tRNA            complement(27653..27729)
                     /locus_tag="KLH46_000424"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27693..27695),aa:Met,seq:cat)
     gene            28077..29420
                     /gene="argG"
                     /locus_tag="KLH46_000425"
     CDS             28077..29420
                     /gene="argG"
                     /locus_tag="KLH46_000425"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBE0377247.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29428..31035)
                     /locus_tag="KLH46_000426"
                     /pseudo
     CDS             complement(29428..31035)
                     /locus_tag="KLH46_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31513..31599)
                     /locus_tag="KLH46_000427"
     tRNA            complement(31513..31599)
                     /locus_tag="KLH46_000427"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31563..31565),aa:Leu,seq:gag)
     gene            complement(31614..31946)
                     /gene="secG"
                     /locus_tag="KLH46_000428"
     CDS             complement(31614..31946)
                     /gene="secG"
                     /locus_tag="KLH46_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBE0377248.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="KLH46_000429"
     CDS             complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="KLH46_000429"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBE0377249.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33504..34352)
                     /gene="folP"
                     /locus_tag="KLH46_000430"
     CDS             complement(33504..34352)
                     /gene="folP"
                     /locus_tag="KLH46_000430"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBE0377250.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="KLH46_000431"
     CDS             complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="KLH46_000431"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBE0377251.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="KLH46_000432"
     CDS             complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="KLH46_000432"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBE0377252.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37231..37524
                     /gene="yhbY"
                     /locus_tag="KLH46_000433"
     CDS             37231..37524
                     /gene="yhbY"
                     /locus_tag="KLH46_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBE0377253.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37680..38156)
                     /gene="greA"
                     /locus_tag="KLH46_000434"
     CDS             complement(37680..38156)
                     /gene="greA"
                     /locus_tag="KLH46_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBE0377254.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38404..39837
                     /gene="dacB"
                     /locus_tag="KLH46_000435"
     CDS             38404..39837
                     /gene="dacB"
                     /locus_tag="KLH46_000435"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBE0377255.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="KLH46_000436"
     CDS             complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="KLH46_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBE0377256.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKVIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41211..42176)
                     /locus_tag="KLH46_000437"
     CDS             complement(41211..42176)
                     /locus_tag="KLH46_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBE0377257.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="KLH46_000438"
     CDS             complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="KLH46_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBE0377258.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="KLH46_000439"
     CDS             complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="KLH46_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBE0377259.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43150..44121
                     /gene="ispB"
                     /locus_tag="KLH46_000440"
     CDS             43150..44121
                     /gene="ispB"
                     /locus_tag="KLH46_000440"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBE0377260.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44348..44626
                     /gene="sfsB"
                     /locus_tag="KLH46_000441"
     CDS             44348..44626
                     /gene="sfsB"
                     /locus_tag="KLH46_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBE0377261.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44674..45933)
                     /gene="murA"
                     /locus_tag="KLH46_000442"
     CDS             complement(44674..45933)
                     /gene="murA"
                     /locus_tag="KLH46_000442"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBE0377262.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="KLH46_000443"
     CDS             complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="KLH46_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBE0377263.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="KLH46_000444"
     CDS             complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="KLH46_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBE0377264.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="KLH46_000445"
     CDS             complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="KLH46_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBE0377265.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="KLH46_000446"
     CDS             complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="KLH46_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBE0377266.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="KLH46_000447"
     CDS             complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="KLH46_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBE0377267.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="KLH46_000448"
     CDS             complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="KLH46_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBE0377268.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49714..50679
                     /locus_tag="KLH46_000449"
     CDS             49714..50679
                     /locus_tag="KLH46_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBE0377269.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50693..51679
                     /gene="kdsD"
                     /locus_tag="KLH46_000450"
     CDS             50693..51679
                     /gene="kdsD"
                     /locus_tag="KLH46_000450"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBE0377270.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51700..52266
                     /gene="kdsC"
                     /locus_tag="KLH46_000451"
     CDS             51700..52266
                     /gene="kdsC"
                     /locus_tag="KLH46_000451"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBE0377271.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52263..52838
                     /gene="lptC"
                     /locus_tag="KLH46_000452"
     CDS             52263..52838
                     /gene="lptC"
                     /locus_tag="KLH46_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBE0377272.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52807..53364
                     /gene="lptA"
                     /locus_tag="KLH46_000453"
     CDS             52807..53364
                     /gene="lptA"
                     /locus_tag="KLH46_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBE0377273.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53371..54096
                     /gene="lptB"
                     /locus_tag="KLH46_000454"
     CDS             53371..54096
                     /gene="lptB"
                     /locus_tag="KLH46_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBE0377274.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLISH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54144..55577
                     /gene="rpoN"
                     /locus_tag="KLH46_000455"
     CDS             54144..55577
                     /gene="rpoN"
                     /locus_tag="KLH46_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBE0377275.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55600..55887
                     /gene="hpf"
                     /locus_tag="KLH46_000456"
     CDS             55600..55887
                     /gene="hpf"
                     /locus_tag="KLH46_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBE0377276.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56005..56496
                     /gene="ptsN"
                     /locus_tag="KLH46_000457"
     CDS             56005..56496
                     /gene="ptsN"
                     /locus_tag="KLH46_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBE0377277.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56542..57396
                     /gene="rapZ"
                     /locus_tag="KLH46_000458"
     CDS             56542..57396
                     /gene="rapZ"
                     /locus_tag="KLH46_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBE0377278.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57393..57665
                     /gene="npr"
                     /locus_tag="KLH46_000459"
     CDS             57393..57665
                     /gene="npr"
                     /locus_tag="KLH46_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBE0377279.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57878..58504
                     /gene="yrbL"
                     /locus_tag="KLH46_000460"
     CDS             57878..58504
                     /gene="yrbL"
                     /locus_tag="KLH46_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBE0377280.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGGDKEIRRE
                     LKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCRYE
                     EDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLIPL
                     ATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="KLH46_000461"
                     /pseudo
     CDS             complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="KLH46_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="KLH46_000462"
     CDS             complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="KLH46_000462"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBE0377281.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="KLH46_000463"
     CDS             complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="KLH46_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBE0377282.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLELNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62540..63469)
                     /locus_tag="KLH46_000464"
     CDS             complement(62540..63469)
                     /locus_tag="KLH46_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBE0377283.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAVRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64144..68604
                     /gene="gltB"
                     /locus_tag="KLH46_000465"
     CDS             64144..68604
                     /gene="gltB"
                     /locus_tag="KLH46_000465"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBE0377284.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68617..70035
                     /gene="gltD"
                     /locus_tag="KLH46_000466"
     CDS             68617..70035
                     /gene="gltD"
                     /locus_tag="KLH46_000466"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBE0377285.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70219..70698
                     /locus_tag="KLH46_000467"
     CDS             70219..70698
                     /locus_tag="KLH46_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBE0377286.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17314 a        19178 c        17549 g        16663 t
ORIGIN      
        1 ggttaccgcg tagcctggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
       61 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
      121 aaagtctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
      181 atggaacctt cagtgccgga tacagttacg gtaatgacag cagccaatac aattacggcg
      241 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
      301 cttttgcgct tatcgatgct aacggggctt ctggcgtgag gatacaaaac tatccgggga
      361 ttgctactga tccctttggc tatgcagtgg ttccttatct cacgacttat caggaaaacc
      421 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgaacaa acaacacagt
      481 ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
      541 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
      601 gcaacgatga tacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
      661 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
      721 gtcagtttgc ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacag
      781 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
      841 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagccgacag cactagcggt
      901 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
      961 ggacgggagt ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc
     1021 tctgcgggta aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga
     1081 catcagacag gatattacaa actcaatgac agcctggata ttaaaaccac attacaggca
     1141 aacgacattc caggactcac aaccgaccag gttgtctctg ttaacacccg attcacacag
     1201 ataaaaagca gcaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg
     1261 tctcgttatg gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag
     1321 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcgtgg
     1381 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     1441 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaagtt
     1501 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     1561 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     1621 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccagg
     1681 cgaagaagtt cagacaatgt gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     1741 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     1801 gtcaatatgc gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     1861 cagggcacag ccgccattac cgtcatggtg cggtaaaaga agacgttacg ttttgattaa
     1921 cgcaacgctt cggcactgtt acccctgctg ctccagcgca tacttataca gcgcattttt
     1981 cttcacgcca tgaatttctg cggccagcgc cgccgctttt ttcagcggca gttctgcctg
     2041 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     2101 ttcgacaatc agcaccattt cgcctttgcg acggttttca tcttccttta cccacgccag
     2161 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     2221 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     2281 gtgggtagat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     2341 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagatggtaa
     2401 acccgcggcg cttaacgcag tgatagcagc acacggtccg ggtagcggca ccacacgaat
     2461 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     2521 ggcatcggaa accagcgcaa tgttttgccc ctcttgcagc tttgccagca gcgtttcggc
     2581 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     2641 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     2701 ctctaacgta cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     2761 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     2821 ttaatattga gcattgcgta aaaaaaatat cactggatac attatggtac cctcaacatt
     2881 ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg
     2941 ttgtggcacc catactcccg atcagtccac tgcttatatg cagggcacgg cgcaggctga
     3001 ttctgccttt tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca
     3061 attactcgcc attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt
     3121 taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggc
     3181 agagattaaa ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac
     3241 acctgccgat ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag
     3301 ccaggggcgt ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgcttgg
     3361 cgcgaaagaa aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca
     3421 ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga
     3481 tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc
     3541 cgactggcag aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt
     3601 taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa
     3661 tggccaggca gcggtatttg gtcgcactat tcagcaaggc tttgaagccg cgaaaaatat
     3721 cggcactcag ccagtggcgg ctcaggtagc tgccgcacct gccgcagacg tagcagaaca
     3781 acctcagccg caaactgcgg atagcgttgc cagcccggca caagcctcgg ttagcgatct
     3841 gaccggtgat cagcctgcgg cccagccggt gcctgtaagc gccccggcga caagcaccgc
     3901 agcggtaagc gcacccgcaa atccatccgc agagctgaaa atctacgata cctcatcaca
     3961 accacttagc cagatcttaa gccaggttca gcaggatggc gcgagtattg tggttggtcc
     4021 gttgctgaaa aataacgttg aagagttgct gaagagcaac accccgctga acgtactggc
     4081 actgaaccag ccggagaata tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc
     4141 ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct
     4201 ggtgctgatc ccacgcagtg cattgggcga tcgcgtagcc aatgcgtttg cgcaagagtg
     4261 gcagaaactg ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg
     4321 cgcgggtgtt aacggcggtt ctggtattgc tttaacgggt agcccgatta ctcccagagc
     4381 gacaaccgac tccggcatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga
     4441 ccagttcacc aataatggcg gtcgtgtcga tgcggtctac attgtggcaa cgccgggtga
     4501 aatcgctttt atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaacgct
     4561 gtacgccagt tcccgcagcg cacaaggcac cgcaggcccg gatttccgac tggagatgga
     4621 aggcttgcag tacagcgaaa tcccgatgct ggcaggcggt aatctgccgt taatgcagca
     4681 ggcgctcagc gcggtgaaca acgattattc gctggctcgc atgtatgcga tgggcgttga
     4741 tgcctggtcg ttggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa
     4801 cggtaatacc ggaagtctga cggctaaccc ggattgcgtg attaacagga agttatcatg
     4861 gctacagtac caacaaggtc aggtagtccc cgccagttaa ccaccaaaca gaccggcgat
     4921 gcgtgggaag cacaagcgcg tcgctggctg gaaggcaaag gactgcggtt tatcgccgct
     4981 aacgtgaacg agcgtggcgg cgagatcgat ctgataatgc gtgaaggcct gaccaccgtt
     5041 tttgtcgagg tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc
     5101 cgcagcaaac aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg
     5161 agttttgata ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag
     5221 tggattaagg atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga
     5281 aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct
     5341 tccggatgcc atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa
     5401 aatcctctgt tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat
     5461 gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa
     5521 tgttgtctta acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt
     5581 gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg
     5641 cgatattgtt aaagcagttg aagccgccgt tacgcgtgat atgaccattg tggcattgac
     5701 cggctatgac ggcggcgaac ttgcaggttt gttagggcca caggatgtgg agatccgcat
     5761 tccttcgcat cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg
     5821 cgatctgatc gataacacgc ttttccctca ccaggatgat taaggagaat acatgaaggc
     5881 attatcgcca atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc
     5941 cgtagtgggt accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac
     6001 ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca
     6061 gattaagaaa gaaacccgta ttaatgtcac ggcctatcag ggcaaagtgc tgctggttgg
     6121 gcagtcacca aatgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg
     6181 tgccaacgaa gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcatc
     6241 taacgatacg tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa
     6301 atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac
     6361 tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg taaagcgggt
     6421 taccacagcg tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc
     6481 cgcgtcttat caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca
     6541 tccgacatgt tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga
     6601 cactgccacc atcgcccctg tcagccataa ggctttcgcc gggaacgctt tgcgcagcat
     6661 aatcagtgac ggcaaactca ctgccgggag cgtcatcaac aatgccagcg ccggagcggt
     6721 tcccatacct gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac
     6781 aaacaagcat cctgctaccg ccatcgccac cacccacatc aggctgttat cgacagcacc
     6841 atcggcatgg gggaataacc agacgcgtgc ggcacccaac accagtactg caaggatgca
     6901 aaccgggatc gtactccaga aaagtgtcca tagcgccctg ccccagcggc taaaaaaacc
     6961 gctctgtgct tccggtatgt caatttcgac cggtgcctgc gtttgcagtg tttcacgcac
     7021 ccatttttgc accagcgtcg caatcagcaa cgccatcacc agcccggcca ccagacgaat
     7081 cgccgcaaaa ccccagccga ggacaaaccc cataaacacc agcgtcgccg ggtttaacac
     7141 cggattgccc atccagaatg ccagcgcacc gcccatcgat acctgttggc gacgcattcc
     7201 cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcagcgaaa acagcgttcc
     7261 cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat
     7321 cagcacctga atcaacgaac cgaggatcac ccccagcacc gccgctttcc atactgcgag
     7381 gaaatagatc atcgcgtaat ccaacgccgc ttgccacgga ttagcatccg cctgcgcaag
     7441 gatagattta ccgatactgt gggtttcggc tgcagtaaac gctttgccgt agtaaggttg
     7501 ccatttcaca taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca
     7561 ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa
     7621 gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc
     7681 agctcttttg cgtaattctg aagagcttaa aatcgtcacg ccttcatgct caggccgcat
     7741 cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttacctgg
     7801 taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc
     7861 cagtaacatc gacgggcgag caatggtcaa tttcggccag ttctgggcaa ttaatgcttc
     7921 ttccatctcc cctttgacgc ggttatagaa aaacggcgag tgggcattgg cacccatagc
     7981 actgaccacc agcatatgct gcgcgcccag tcgccgcccg gttaatgcgg tatccactac
     8041 cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc
     8101 gagacaacaa aacacaatgt cgatgggttc ggtgacctgc gccaacgcgt cactcagttg
     8161 cggatcatgg ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg tcggcgcagc
     8221 aatggcgtta actttcggtt cattaatcaa catccgcagc aggtgaccgc ccaccagccc
     8281 cgttgcgcct gtaatcagta cctgactcat cttcgctcct ttacagaatt gtctgccttg
     8341 cgctccccgg ctcatgcacc agtcttaata gaccatgagg taattatccc cgattgtgga
     8401 aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt
     8461 aatcactgat gaatttgaag attcagaatt tacttcaccc gcagacgagt tccgtaaagc
     8521 cggacacgaa gtgattacta ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg
     8581 agaagccagc gtgaccatcg ataaatccat cgatgaagtg acgccagcgg agtttgatgc
     8641 cctgttgcta ccgggtggtc attcaccgga ttatctgcgt ggcgacaacc gttttgtcac
     8701 ctttacccgt gattttgtga atagcggcaa acctgttttc gccatctgtc acggtccgca
     8761 gctactgatc agcgccgatg tgattcgggg gcgcaaactg accgcagtta aaccgatcat
     8821 tattgatgtt aaaaatgcgg gcgtggaatt ttacgatcag gaagtcgtgg ttgataaaga
     8881 tcagctggtt accagccgga caccggacga tctgccagcg tttaaccgcg aggcgttacg
     8941 cctgctcggt gcctgagtcg cgtagccaaa tcattttttt accaaagccc agcgtgttgt
     9001 cggtgaattt gatttcatcg agccggattt cccacaccgg ggccgacagc attctggcaa
     9061 ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc
     9121 ggatctcacc tttaaactgc acaccgcgaa ttaacgctac cgttttcggc tgaccgttta
     9181 ccgttccggc aaccgcagcc tgcggcccac tcatctgcgc gtggcgcgtt ttttcttccg
     9241 tcaaaatgta gaaggcgact ttctgcgcat caaaaagata aaaggcatta gcgcaccaga
     9301 gttccccttc ctgctgcaca caccaggtga caacatgttg ttttgccagc caacggctga
     9361 tggcgatgag tgtttccatt gctgttctct tttatactgt gggcctgacc ttaacatgcg
     9421 atgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacaa taagctttat
     9481 accgggatca ccacggatgt cgaacgccgc tatcagcagc accaaagcgg caaaggggcg
     9541 aaagcactgc gcgggaaagg agaactgacg ctggcgtttt ctgcgccagt gggcgaccgt
     9601 tcgctggcac tacgggcgga atatcgcgtt aagcaactga ccaaacggca aaaagagcgt
     9661 ctggtagcgg aaggcgcagg gtttgcagaa ctgttgagca gcctgcaaac cccggagatt
     9721 aaaagcgatt gaaatgctcg tgatactcaa ccagaccggt tacgccattc agcgcgtcat
     9781 ccgctaaacg atgtaccagg aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt
     9841 gatgcgccgc cagttcaaag ccgaaacgac tgtacagcgc cggatcgccc agcgtcacca
     9901 ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt tggcgtgcca
     9961 gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc catgccgacc cattgcaggt
    10021 cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga ccttcgtcat
    10081 ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga accagcttcg
    10141 cttccgcatc actttcgaat gagcgacgca gcagggcatc aatacccggc gcatcaatgg
    10201 gaatttctac tcgaattagc atggttcacc taccgatgtc tgtttggttt caggcgcggt
    10261 tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta aggctttcgg
    10321 catttgctcc agttcaatgg cgtccatcag gtttttcaca tacaccccca gctccgtatc
    10381 gccttcaatc accagccgac gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc
    10441 aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg tgttctggct
    10501 gaccaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat cacgcacatg
    10561 aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccagcg cctgacggaa
    10621 ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg tcagttttac
    10681 cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt tatccaacac
    10741 gagttttact ccctgtttca acaatcatcc tattttgcca tatcagaaaa ataacatagc
    10801 ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact ttaactgcct
    10861 taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac atttttgcgt
    10921 tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg gaaatctccc
    10981 ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc taaaagatga
    11041 taccaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc aggaagcggt
    11101 gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt ttgcgcatcc
    11161 ggacggttac gcccgttggc agcgtgccgt ggatatggcg gcgcagctgg gtgccgacgc
    11221 gctgatcctc gccgacctcg ccatgctgga gtacgccgcc gagcgttacc cgcatattga
    11281 acgtcatgtg tcagtgcagg cttcggcgac caatgaagag gcgattaact tttatcatcg
    11341 ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca
    11401 gctggcacgg gttactcctg tcccgctgga agtgtttgct ttcggcagcc tgtgcattat
    11461 gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca acactgtggg
    11521 tgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg ccgcaggggc tggaatcccg
    11581 cctgaacgaa gtgctgatcg accgttatca ggacggcgaa aacgcaggtt atccgacgct
    11641 gtgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag aaccaaccag
    11701 tctcaatacc ctggaactgc tgccggagtt aatggctgcg aatattgctt cggtgaaaat
    11761 tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg gcgaaagtct ggcgtcaggc
    11821 tatcgaccgt tgtaaggccg atccgcaaaa cttcgtaccg caaagcgcgt ggatggagac
    11881 gctcgggtcg atgtccgaag gcacgcaaac cacccttggc gcgtatcacc gtaaatggca
    11941 gtgagaagag caatgaaata ttccttaggg ccagtgctgt ggtactggcc aaaagagacg
    12001 ctggaagatt tttatcagca agccgccgcc agcagcgccg acgtgattta tcttggtgaa
    12061 gcggtatgca gcaagcgccg ggcaaccaaa gttggcgact gtctggagat ggcaaaatcg
    12121 ctcgccggaa gtggcaagca gattgtgctc tccacgctgg cgctggtgca ggcatcctct
    12181 gaactgggcg aactgaaacg ctatgttgag aacggtgaat ttctgattga agccagcgat
    12241 ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg
    12301 aactgctaca acgcggtgac gctgaaaata ttgctcaaac agggcatgat gcgctggtgt
    12361 atgccggtgg agctttcccg cgactggctg gtgaatctgc ttaatcagtg cgatgagctg
    12421 ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctacc gctggcctac
    12481 tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc
    12541 tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta
    12601 ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga gctggcatcc
    12661 atgcagggat tggttgatgt agtccgcctg tcaccgcagg gcactgacac tttcgcgatg
    12721 ctcgacgcct tccgcgctaa tgaaaatggc gctgtgccac tgccgttgac ggcaaacagc
    12781 gactgtaacg gctactggcg gcggctggca ggactggagc tgcaagccta agtaaatagc
    12841 tcactttgtt aacaacttta actattcttt aatgcagtat taaagattaa tcggtaacaa
    12901 agtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg gcccccatac
    12961 ccgaaggctc ttcagcgcgg gaagcgttct cccactctct cgatctcgcc cgtctggctg
    13021 aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgacc ggcattgcca
    13081 gtgctgcaac atcggtattg attggctatc tggcggcgaa taccaccacg ctgcatctgg
    13141 ggtctggcgg cgtgatgttg cctaaccact taccgttggt cattgccgaa cagttcggca
    13201 cactcaatac gctctatccg gggcgaatcg atttggggct gggtcgtgct ccgggtagtg
    13261 accaacggac aatgatggcg ctacgtcgtc atatgagcgg cgatattgat aatttccccc
    13321 gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg catgtgcgcc
    13381 cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagc ctttacagtg
    13441 cacaactggc ggcgcagctt ggtctgccat ttgcgtttgc ctcacacttc gcgccggata
    13501 tgttgttcca ggcgctgcat ctttatcgca gtaacttcaa accgtcggct cggctggaaa
    13561 aaccatacgc gatggtgtgc atcaatatta tcgccgccga cagcaaccgc gacgctgaat
    13621 ttctgtttac ctcaatgcag caagcctttg tgaagctgcg ccgtggcgaa accgggcaac
    13681 tgccgccgcc gattcaaaat atggatcagt tctggtcacc gtctgagcag tatgacgtgc
    13741 agcaggcgct gagtatgtcg ttggtaggcg ataaagcgaa agtgcgtcat ggtttgcagt
    13801 cgatcctgcg cgaaaccgac gctgatgaga ttatggttaa cgggcagatt ttcgaccacc
    13861 aggcgcgcct gcattcgttt gagctggcga tggatgttaa ggaagagttg ttgggatagt
    13921 gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct gtcctacgta ggcctgataa
    13981 gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac cgctgattac
    14041 tgatacaccg gcagtaaatt aaagctcgat aaaatatgca ccagtgcgtt gccgacgcca
    14101 aacaccagaa tcagcgcaat catcggcttg ccaccccaga cgcggaattt cgggctgcca
    14161 aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca gatggtagcc
    14221 gctaaaccag cataaccaat ggcatacagg aatccgttcg ggaacagcag accacccaca
    14281 acaggcgggg caaaggtcag caatgccgtt ttcaagcggc ccacagccga gtcgtcgaaa
    14341 ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa cgaactcgct
    14401 accgcaaagt ttgagaacac gaccagcaga aggtccaggc tacggctgtt cagcacgccg
    14461 cttaatgctt gtaccaacac atcaatatta ccgcccttct cggcgatgcc gataaactcc
    14521 ggacgaggga tgttgcccat tgtcgccagc aaccagatgg tatacagcgc cagtgccatc
    14581 agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta atacttcatc
    14641 aggcttggca cgttaccgtg ataaccaaac gacgccaggc agaacggcag ggtcatcagc
    14701 aaatacggcg cataagatgc attgctttcg gcaacgttga acaatgtcgc aggctgcaca
    14761 tgccccagca ggctaccgaa ggtgaggaag aaggtaatga ctttcgcccc cagcacaatc
    14821 gccgtcatgc ggctgacggc tttagtactc aaccacacca caaacgctac cagcagtgca
    14881 aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca tctctgcgaa ggtgtgatgc
    14941 agaatcgaac cactggcaga aatataggca taggtcagga tatagagcac aaaggcaatg
    15001 gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat ggtgtcaaaa
    15061 ctcgaaccga ttcggtaatt caggttagct tccagaatca tcaagccgga atgcagcata
    15121 cagaaccagg taaagatcag cgctgccatt gaccagaaaa accacgcccc ggacatgacc
    15181 actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac cacgccgcca
    15241 agcagcgacg gtgacgtttg ggtggtggtt cgtgttgcca tgagggcttc tctccagtga
    15301 aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacatgagt acaaaagaca
    15361 gaaaaaaagc cccgatggtt aaaatcgggg ctgtatatat tattttacag attgtgttcg
    15421 ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga gtcatcacga
    15481 cgcggtgcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc acgaccacct
    15541 tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gctcgccacc gaaaccacga
    15601 ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa ctgcatgttc
    15661 atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc cggcatacct
    15721 ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccgat gtaacggctg
    15781 ctgatgtcgc cttcgttagc aatcgcgcca acgatatgac gaacttcaac accatcatcg
    15841 cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc acgacgcgga
    15901 cgatcttcac ggtcgccacg cgggccacgg tcgttacgat cgcgcggacc acggtcatca
    15961 cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat cagagtacgt
    16021 tcaccctgtg ccattttcag cagtgccgca gccagagttt cgagatccag ctcttcacct
    16081 tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc gctgctttcc
    16141 agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag cagttctgcg
    16201 ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt gcgcagcaga
    16261 cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc acgaccggta
    16321 cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt aaccaccagg
    16381 ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag gatgtccaga
    16441 cgaccgtctt taaggcgttc cagtgtctgc tcacgcagcg cctggttcat gtcaccgttc
    16501 agcgcggcgc tattgtagcc gttacgctca agagcttcag ccacttccag agtcgcgttt
    16561 ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa acgtaccagt
    16621 gcttcgtttt tacgcatacc ccacacagtc cagtagctct ggctgatgtc aggacgggta
    16681 gtcacgctgg actgaatacg cacttcctgc ggctctttca taaagcggcg ggtaatgcga
    16741 cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc cgggatctgc
    16801 gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc
    16861 agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg gtccagcaga
    16921 cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa ttgcacgtca
    16981 taacgctggc cgccgtacag ggcaaccaca tttacgccgc gcatgtgttt agagaaatcc
    17041 gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac cagaatctgt
    17101 ggtgctttca gctcaggatc aagattctgc agcagaggta aagagaatgc tgcagttttt
    17161 ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg tggaatacac
    17221 tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc aaggatagga
    17281 gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc
    17341 aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa ttttcattga
    17401 aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc acccgttaag
    17461 gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc tggtccgatt
    17521 ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg tatcggtgct
    17581 caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca
    17641 aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg agatgctcag
    17701 cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa
    17761 tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc gatttttcga
    17821 agtgctgttt caatacttct ttagcctgct tctcatcgag cttctgctcg gcgagataaa
    17881 gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc agatcatctt
    17941 gcgctaactt gtcacgaccg ccgtaatata atgcaatccc gcgattcaag tgcgcgtagt
    18001 tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca tcaaaattgc
    18061 ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc ggtcggattg
    18121 ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta tcatacaaca
    18181 ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga
    18241 tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat ggtaccgcga
    18301 ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa
    18361 cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg ttgaatgaac
    18421 gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg
    18481 agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt gctgcagcag
    18541 gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg cgatcaactt
    18601 ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact ttctcaacgc
    18661 gtttgtcagc gatttgagag atgtgtacca gaccttcttt accgccgccg atggcaacaa
    18721 atgcgccaaa gtcaacgata cgggtcactt taccagtgta gacgcggccc acttcgattt
    18781 ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg tcggtcgctg
    18841 caatcttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct tcggtcatgg
    18901 cacggataac agagccgcct ttaccgataa cgtctttgat cttgtccggg ttgatcttga
    18961 tggtatggat acgtggtgcg aactcagaga tatcgccacg cggcgcgttg atcgcctgtt
    19021 ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagcgca acctgcatga
    19081 tctctttggt gataccttca attttgatat ccatctgcaa tgcagagata ccctcgcggg
    19141 aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca
    19201 gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca acggcagctt
    19261 tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa gccatagaag
    19321 aggaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg aatttgtcca
    19381 tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca atttcacgac
    19441 gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag ttgtagtgga
    19501 acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg tcacgagcag
    19561 tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc gcagaaccgt
    19621 gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct ttttcacgac
    19681 cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc tcgatcgcgt
    19741 gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc agcgtcgcga
    19801 tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt
    19861 aagcatcgct caggcgagct tcagccagtg cagcaacgcg cgcgttcagc gcttcgttta
    19921 ccggctccgg ctgccagtcc caacgtggtt taccggcttc tttcaccagt tcattgatgt
    19981 tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc atctggtctt
    20041 cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta ccggcaacaa
    20101 ccagatccag tttgctctct ttcaactcgt cctgagtcgg gttcagtacg tactggtcat
    20161 tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa cggaatacca gacagagaca
    20221 gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacttgcggg ttaacagaaa
    20281 ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc ggccgaatcg
    20341 ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct tcacgacgga
    20401 agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta acggtcagtg
    20461 ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca aataccgcgg
    20521 tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg ccggtttcca
    20581 gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc aatgtaatat
    20641 cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat cttctgcgca
    20701 tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc tcattagccg
    20761 cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc cagtgaattg
    20821 ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact cgcaagaatt
    20881 agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct ttacgtttca
    20941 ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga cggctgtggt
    21001 gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca gtcagcagtg
    21061 ctacctgaac ttcagtagaa ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt
    21121 tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag aatgaaagga
    21181 cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa caatttacgc
    21241 gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg ttaacattac
    21301 gccgggtatt caaccaccag gcgacgaggc gcaacgcggc cttcatcgtc aatttcgccc
    21361 ataccgataa atttgccgtt ctcaccttct gtaacgcgaa ccagtccttc cagcggcgca
    21421 ccggatgtac gaactgggtt accatttttg aagtaaacag aagacgttaa cggaagattc
    21481 accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc aagtaactcc
    21541 gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt cacgcagatg ttccagggtc
    21601 acaatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat aacatgcgcg
    21661 ccacaaccga gtttttcacc caggtcatca atgatggtgc ggatataagt gccttttgag
    21721 cagtgaattt ccagctccag ctcattgcct tcgtggcgaa taaacagcaa ttcataaacg
    21781 gtaatcgggc gcgcttcacg cggtacttca atgccctgac gcgcatattc gtacagtttt
    21841 ttaccctgat atttcagtgc tgaatacatc gaagggatct gttcgatatc gccacggaaa
    21901 gtatccagcg ccgccgccag ttgctctgcg ctaaaggtta ccggacgttc ttcaacgatt
    21961 tgtccgtcgg catcagaagt atcggtacgc tgtccaagac gcgcaatgac ccgatagcgt
    22021 ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca aattggcaac
    22081 atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc gttataaatc
    22141 cgtttcactt tttgcagcgc atcattgctg gacataccct gcggtttatc cagcaacaaa
    22201 acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt
    22261 ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc
    22321 gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc
    22381 gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcttgcaac gctttgatgc
    22441 ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt cacatatact ttggcatacg
    22501 ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccagg cgaggatctt
    22561 taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct acgcgctgcg
    22621 ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct gttagcccct
    22681 ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgccagcg
    22741 tcacatcagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat ggaaccctaa
    22801 aaaccttaag cgatggtacg ttggatctcg atgatttcga atacttcgat cacatcgcca
    22861 gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg
    22921 ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat aaccacgttg
    22981 tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacagcct
    23041 gcgatggcac caaatttcgg cgatttgaac acgtcacgaa cttccgccag accgataatc
    23101 tgctgtttca gttccggaga cagcataccg ctcatcgccg ctttcacttc gtcaatcagg
    23161 ttatagatga cggagtagta acgcagatcc aggctttccg cttcaatcac tttacgtgca
    23221 gaggcatcag cacgtacgtt gaagccaacc aggatggcgt tggacgccgc agccagggtg
    23281 gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca
    23341 gtagacagtt tcagcaagga gtcggagatc gcttccacag aaccctgtac gtctgccttc
    23401 aggacgatgt tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt
    23461 ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg atacagtgca
    23521 acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggtacg
    23581 ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac ttcctgaccc
    23641 agttcgttac gcatcgcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc
    23701 ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg
    23761 aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag ctccagaact
    23821 tccgcctgca gcaggatagc gtccagcagt tcatcgatac cagtacccgc tttcgcagac
    23881 acatgtacga actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt
    23941 tcgttcttaa cgcgatccgg atcggcttct ggtttatcga tcttgttcac tgcaacgacc
    24001 accggaacct gcgccgcttt cgcgtgctgg attgcttcga tcgtctgcgg cattacgccg
    24061 tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg agcacgcatt
    24121 gaagtaaacg cggcgtgccc cggggtgtcc aggaaggtga tcatgccgtt ttcagtttca
    24181 acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt
    24241 gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca
    24301 accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt cgctcattac cgcctcttcc
    24361 agctcgtttt cacgacgcag gataacttta tggcccatct cttcagcaac cagctgtgcg
    24421 gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat catcgctttg
    24481 atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatagtttcg
    24541 ccgatcacaa cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa
    24601 gaacctttac gttttccgcc tttaccgcca cgtactgctg cgcgtgcttc ttcacgatca
    24661 gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc gttacgacca
    24721 cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga
    24781 gaagtagtga cgtgataatc gctggaatct tcagtcggtt ccgcgttatc agtccatttg
    24841 ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta
    24901 cgacgcgctt cttcttcagc tttacgcttg agctctgcag cttcctgctc acggcgggct
    24961 ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc
    25021 gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt
    25081 tcagcttttt gttgcgcctc gcgtttagcc gattcttctg cctcacgacg ggcttgctct
    25141 tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca caaaggtgcg tttcttgcgg
    25201 acttcgattt gtaccgattt gctttttcca ccggtaccag gaatgttaag ggtgctgcgt
    25261 gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt tctgattcag gtggtcaatc
    25321 aaagtctgtt tctcttgtgc agacacagag tcgtcagcag acttccggat acctgcatca
    25381 gcaaattgct gtaccaggcg ttccacggag gtctgtcgct ctgcggccag cgttttaatc
    25441 gttacatctg tcatgctgtt ccttcctgct acagtttatt acgcttcgtc accgaaccag
    25501 caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa cccttcgata
    25561 tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac gccacgggcg
    25621 gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc gtcagccggt
    25681 ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc atttttagca
    25741 cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa cagctctttc
    25801 atcggcacat aggccagttc ttccagcgtc gagaaacctt cttctaccag aacagtcgcg
    25861 aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg cgcttccgcc
    25921 tgatgcttag cctgcaggtc gtcaacggtc attacgttga gttcccaacc gctcagctgc
    25981 gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccaggtt accagcttca
    26041 acggcgatat ccatggtgtg tttatcttca tccaccacga tagaagcaac gtctgccggt
    26101 gccattgcgt taatcacaaa ctgcgccggg ttatcatccc acaggacgat atcgatacgc
    26161 tcgccgccca gttcagtaga aaccgcctga acacgcgcgc cacgcatacc tacgcaagca
    26221 cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg agaacccggg
    26281 tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttccggcac ttcaatacgg
    26341 aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc gccacgcgct
    26401 tccgggcgaa cggaataaag cacgccacga acgcggtcgc cagggcggaa gttttcacgc
    26461 ggcagcatat cttcgcgcag gatcacggct tcagcgttgt tgcccagatc cagagagatg
    26521 ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg ttcacggaac
    26581 tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac ctgttttgcc
    26641 gtctgggtgg tgatacggtc aaaggtaaca gactcaatct gatcttcaac gtaatcgccc
    26701 aagttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt ggtcggctgg
    26761 gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa aatcaccgct tttgcgatcg
    26821 atctgtacac ggacgtcgat ctcttgctca tattttttct ttgttgctgt cgccagcgcg
    26881 ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga tacggcttca
    26941 actacagcca aaatttcttt gttcatcgcg ggcttttcac ctcatccaga ctattaaaag
    27001 tggggaacca ggttcgcctt ctggatatta ctcagcgcga acacttcatc tttaccttcg
    27061 acggtaactg tgatcatttc accgtctacc gctttgataa cgccctgcca tttacgacgg
    27121 ttttgtaccg ccatacggag aaccagagtc acctcttctc cgacaaaacg ggcgtagtgt
    27181 tcagccgtga acagtgggcg atcgagaccc ggtgaggaga cttccaggtt ataagcaacg
    27241 gtgatgggat cttcaacatc cagcacagca cttacctggt ggctcacatc agcacaatca
    27301 tcaacattga tgccatcttc actatcaata tagatgcgca gtgtggatgt gcgaccgcga
    27361 ataaattcga tgcctaccag ttcaaaaccc agggcctcaa ctggcgcagt aatcatctct
    27421 gttaattttt gctctaatgt ggacaagccc acccccaaga cataaaaaaa gggcctaaag
    27481 cccagttatt ctgtagtcag ataacaaaaa accccgataa atcggggctt tatataactg
    27541 aaccctataa ccgcaactgc ggtctggagc actttccaga aggatttttt caaatcccac
    27601 tacgaaggcc gaagtcttca cagtatattt gaaaaaggac tctaagggaa agtggttgcg
    27661 ggggccggat ttgaaccgac gaccttcggg ttatgagccc gacgagctac caagctgctc
    27721 caccccgcgc ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc
    27781 tgggatttgg tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca
    27841 cattttgtca acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt
    27901 gaatactttt gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa
    27961 actcttcctg tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat
    28021 tcagttagag cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga
    28081 cgacgattct caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc
    28141 tggacaccag tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata
    28201 ctgcaaacct gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg
    28261 aatacggcgc ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta
    28321 ttgccgctat tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca
    28381 cgacgccgct gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg
    28441 gcgtgaatat ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc
    28501 gttatggtct gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact
    28561 ttattgatga actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg
    28621 actacaaaat gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc
    28681 atgaagcgaa ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg
    28741 gcgtgaaatt ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg
    28801 agcaaggtca tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc
    28861 tggaagctaa ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc
    28921 gtatcatcga agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca
    28981 ttgcgtatga acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg
    29041 cgcatggtcg tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc
    29101 tgatgctgcg tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc
    29161 tggagctgcg ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct
    29221 acaagccaga gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta
    29281 ttggtcaatt gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt
    29341 atgccaaaac tggcctgctt tcctcctctg ccacttcagg cctgccgcag gtggagaata
    29401 tggaaaacaa aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg
    29461 cttatcctct gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc
    29521 cacaacccgt gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc
    29581 cggctttgtt acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat
    29641 attgttttcc gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat
    29701 cgggacatga tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt
    29761 cgtagggtca cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt
    29821 taataattca aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca
    29881 ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc
    29941 cggttcatgg cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg
    30001 taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc
    30061 catggcgcgc atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga
    30121 gcttagccag aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta
    30181 attatgaatg tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc
    30241 agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc
    30301 aacttgcggt gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga
    30361 ctcccccaca atcaacacgt aggtatcaat acttgtatcc ctgactgata attgaaaata
    30421 cggcaccgtg tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata
    30481 gttgagattg aaaaacggcg tataggtagc aaatcacgac gctagtatat atggactgaa
    30541 cgctttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga
    30601 aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt
    30661 tattattact gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat aagggatata
    30721 catccccagc attttcacaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc
    30781 atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc
    30841 agtcagtaac acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt
    30901 aacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg ctccggaaga
    30961 ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt
    31021 aaaatcttct agcaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata
    31081 taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata
    31141 tcagaccgaa acactgttag ttttttcttt ttaaatgatg cgaccattgc gttctcagta
    31201 attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta
    31261 tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact
    31321 ggagttgaca caataaagtg ccaattatgt cagcagtagg gaaaagttta taactaaggc
    31381 gtaccggcac catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga
    31441 tacctgacaa ccattcatca gaccaggacc aaaacgaaaa gagacgcttt tcagcgtctc
    31501 ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
    31561 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
    31621 gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggt
    31681 gcactcagat tttcccattc gctacctcta ttggttttgt tgctattgat gttacccagc
    31741 accagactga tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta
    31801 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
    31861 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg
    31921 aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
    31981 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
    32041 taaccaaaac gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa
    32101 aaacagcaaa accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca
    32161 gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
    32221 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
    32281 gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa
    32341 ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta acgtacgtta accagaatct
    32401 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg
    32461 ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga
    32521 tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
    32581 cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt
    32641 caagccccat gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa
    32701 gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt
    32761 caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag
    32821 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
    32881 caccgtttgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg
    32941 gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc
    33001 taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga
    33061 tacggctggc tttacccagt tctgccgaat caacgcagct gatctccttt tccatttccg
    33121 cttcgatggc ctcttctacc gcatccggca gtttagtgcc gtcgatagag aagaatttaa
    33181 tcccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga
    33241 aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa
    33301 ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg
    33361 tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
    33421 aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc
    33481 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
    33541 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc
    33601 gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc
    33661 cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg
    33721 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
    33781 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
    33841 cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga
    33901 gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
    33961 tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcatt aataatgtga
    34021 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag
    34081 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca
    34141 acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg
    34201 ccagcattga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca
    34261 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
    34321 tccagtgaag taccctgggc aaagagtttc attatgttat ccctggtatg aattgataag
    34381 aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa
    34441 cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
    34501 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
    34561 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
    34621 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt
    34681 cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt
    34741 cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga
    34801 atacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
    34861 gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag
    34921 aaacatgttc cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa
    34981 tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag
    35041 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt atgcaccgga tcgtgttccg
    35101 gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat tctttctgcg
    35161 cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct
    35221 cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
    35281 tcaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg
    35341 cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac
    35401 cgcgaacatc tggcaagccg accacaacct ggcggtcgaa acggccagga cgcagcaggg
    35461 ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
    35521 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
    35581 cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
    35641 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
    35701 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
    35761 caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt
    35821 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt
    35881 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
    35941 gatcttccgt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
    36001 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc
    36061 aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
    36121 cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
    36181 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
    36241 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
    36301 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
    36361 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
    36421 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
    36481 gtttacgccc ggtcgctaca atatacactt ctcgcgaacg tgcacgagaa gagtccggct
    36541 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
    36601 cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
    36661 ctagcgccag ttccaccaga tacatggctc gggggatatc caccgccggt gttccgctca
    36721 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
    36781 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
    36841 gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca
    36901 catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa
    36961 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaatccct
    37021 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
    37081 tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa
    37141 ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg
    37201 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
    37261 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
    37321 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
    37381 atcgccaccg aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
    37441 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
    37501 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa
    37561 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
    37621 gcagagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct
    37681 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    37741 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    37801 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    37861 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    37921 ttggtgacat caatcacctg cgcgtttgac agcttggctt cgatgtcttt gatacggcct
    37981 tcgcagaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    38041 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    38101 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    38161 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    38221 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    38281 ggtcagctac aattcacatt ttgatggtca ttttaccctg aagttcccga agggtcatcg
    38341 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    38401 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    38461 gccgcgaatg ttgatgagta cgttactcaa ctccccgcag gtgccaacct tgccctgatg
    38521 gtgcaaaaag tcggtgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    38581 cccgccagta cccagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    38641 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    38701 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    38761 gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    38821 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    38881 tttagcgctc cgcctgctgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac
    38941 agtgccccaa agcctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    39001 atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg
    39061 gatgtggtgc caagcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    39121 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg tgcaattctg
    39181 aaagatgagt taaaacaggc gggtatcacc tggagcggaa cactgctgcg ccagactcag
    39241 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt
    39301 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgatcggc
    39361 catgcgcgct tcaatgtgcc tggaacatgg cgggctgggt cggacgccgt gcgtcagatc
    39421 ctgcgccagc aagccggtgt cgatattggt aacaccatta ttgccgatgg ttcagggctt
    39481 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa
    39541 cacgacaatg aacttaactt tatctccatg ctgccgcttg cgggctatga cggctctttg
    39601 cagtaccgtg caggtctgca tcaggcgggc gtggatggca aagtctcagc gaaaaccggt
    39661 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    39721 gcatttgtac aatatctttc tggctatgca gtaaaacccg cggatcagcg taatcgccgt
    39781 attccgttag tgcgttttga aagccgtttg tacaaagata tttatcagaa caattagtca
    39841 aaaagaaacc ccggcgcatg gccggggttt cagattattg acaaagtgcg ctttgtttat
    39901 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc gtgcaaattc aatatattgc
    39961 aatcatcttg taggcctgat aagtgtagcg catcaggctt atttggcatt ggtcattagt
    40021 gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc
    40081 cagtcttcat catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac
    40141 atgaattcga ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg
    40201 ataaaggtca tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc
    40261 agataatatt tatcttccca gcccagcgct tcagcgatca ctttcgcttt ctcttcggct
    40321 tctgccttat ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga
    40381 tcctggctgt atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg
    40441 ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc
    40501 aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg
    40561 gcaacaacga agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg
    40621 gtaaacggat aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat
    40681 ttacccgcgt ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc
    40741 agctcacgct tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac
    40801 gatttgaaac gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga
    40861 ccgtgtttag tcatatcacc catggtttca ccagtaccct ggtcgattac acgcgtacct
    40921 accggcactt taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg
    40981 ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg
    41041 ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg
    41101 cctttcggaa tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca
    41161 acgaccagaa tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct
    41221 gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca
    41281 atggcggaat acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt
    41341 aacatcggtc tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc
    41401 gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc
    41461 gcttccgcca aggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg
    41521 agctgccagt ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcgatt
    41581 gtacataaag tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc
    41641 ttttgcgcca cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc
    41701 caggtgtaat cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc
    41761 agaccgctca ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg
    41821 ataaatacgc tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg
    41881 gtcggactca ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg
    41941 gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac
    42001 ctcttcttca ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac
    42061 acgattgtcg gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc
    42121 cagcaaattg ctgtggtaag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg
    42181 tattccctgc aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat
    42241 cgaatgtaaa aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa
    42301 acttattcag cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc
    42361 actttaccgt ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca
    42421 gcgtggaatt tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca
    42481 ccgaaacgct taacgcccaa gcgtttagct tctgaatcgc gaccgttacg tgtggagccg
    42541 ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa
    42601 ttttcacatc agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac
    42661 gaaacttaac gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta
    42721 cgccgccatc aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca
    42781 cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct
    42841 gaccttcgct tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact
    42901 ccgcttccgc gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc
    42961 gcgaatatta cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa
    43021 aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatt accctatatt
    43081 cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg
    43141 gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt
    43201 aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat
    43261 tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga
    43321 gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga gtttatccac
    43381 acggcgactc tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct
    43441 accgccaacg ccgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc
    43501 cgcgcgttcc agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa
    43561 gctgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa cgatccggac
    43621 atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct
    43681 gccgcgcagt gttccgggat tctggctggc tgtacgccgg aggaggagaa aggcctgcag
    43741 gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat
    43801 gccgatggcg aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg
    43861 ctgccgctgc tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc
    43921 gccatcgaac aaggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct
    43981 tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca
    44041 gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc
    44101 gctgttcaac gcgatcgtta atcccctccc ttcatcccgc gcagtgcgcg ggatgattcc
    44161 agtaaattcc ataaaaacac taattcagcg ctataacacc tgaaaaaagg gcgataaatc
    44221 tttatatatt ctgaatattc acactcttta caggaacttt ttagcgcaat agaccatcag
    44281 gagtatagtg atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg
    44341 aggaaagatg gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg
    44401 caagaaagga acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct
    44461 ggcaaacgca ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg
    44521 aactgacccc tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat
    44581 cgacagaacg cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta
    44641 gccccgcgaa cggggctgcc agctctcaga cgattactcg cctttcacac gctcaatatt
    44701 tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat
    44761 acgatcaacc accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc
    44821 acgcagatcg gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac
    44881 ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa
    44941 gcggttttca aagaccgttt cggtgatgaa cccggtccct tcagccacca ggttcaacag
    45001 cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac
    45061 agccttcgga cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc
    45121 cgctccagca tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg
    45181 gcagataatt ttgccacgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg
    45241 atccggcagc acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg
    45301 atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac
    45361 gatttccggt tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc
    45421 acacatgatg gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt
    45481 cagacgacca tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa
    45541 ttgttcgagg ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg
    45601 tagtgaaact tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc
    45661 acgcatggtt ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat
    45721 atgcacagaa ccattacgtt ctactttcgc acccagctgg cttagcagtt tcattgatgt
    45781 atcgacgtct ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc
    45841 ggcaaaaaga ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag
    45901 cttcgttggc ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat
    45961 atccgctacc ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca
    46021 ctccgcaggg gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc
    46081 catcagcggg ccatagaccg tctgctgttt tttaacccga ctcataccgt caaacaactc
    46141 acccacggca ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga
    46201 gagagcgttc atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag
    46261 ttaataaacc agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc
    46321 tcgctgataa atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg
    46381 ccatatccgg cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt
    46441 cgcgagggta tacactttgt cgtttacccc ctgaagcgtc acgttgttgc cctgcttttt
    46501 cgccagatcg ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg
    46561 gctaagatcg atgcaggtaa tccccttcac cgcctcctca cgcatttccc aaagcggtag
    46621 cagaacgtcc tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct
    46681 cagtgactcg ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc
    46741 agttgcgcag tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt
    46801 ttggtggtga tcatactgac gccttcagca atcatgtcgt aagcttgcca gtttcccgtc
    46861 tgggagtttt tacgccactg gaagtccaga cgcaccggcg gacggccatt cgggtcaata
    46921 atggtaacgc gaataggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga
    46981 taggtttgac cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag gtactcacgg
    47041 aaagcggcaa agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc
    47101 accagcgcac cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta
    47161 cgcagataat ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt
    47221 ttctgcgccg cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca
    47281 ctcagaggtg caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc
    47341 tcctgaaatt atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca
    47401 gctggcgcat cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca
    47461 atgagatctt ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg
    47521 atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttctccc
    47581 agcaggccgg aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt
    47641 tcaatttcca gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc
    47701 cggcccacaa caacaccacc aatgctgacc ggagagcgcg ctttcaggcc gccaatgtta
    47761 tcgaacgtcg cataaagcgt gtaggtcgat tcagtacgta tggacgtcac gttcgccgcc
    47821 ttcaggcaaa caaacagcgc cgccagcagt gctgctaata aaaagatacc cacccaaatt
    47881 tcattttttt tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc
    47941 cagccccaga acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc
    48001 ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc
    48061 gaacaccacg ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg
    48121 cattgccgac cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagcgatcc
    48181 gccccagatc cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taaccccagc
    48241 ccagaaacgg ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga
    48301 gagttgctct gtagcgcgca tcaggccgat ttctgcggtt aacgccgaac cagcacgccc
    48361 ggcaaacaac agcgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag
    48421 catacccaga ctggtttccg cgctataagt ggtcagaacc agataacctt gcagccccaa
    48481 caccattccg atgaacacgc cagaaactac aataatcagc atcgacagga cgccgacatt
    48541 atagagctgg cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc
    48601 attgaataac attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc
    48661 gagcgacgcc agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt
    48721 gataatcgcc ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact
    48781 gacgtacgcg cggatcagga ttcgtctgca acgcctgggc actgccatga gcgacaattt
    48841 ttttgtccgc caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag
    48901 aaaccaccac acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta
    48961 cgcccatggt gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct
    49021 ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag
    49081 gcattagttt agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat
    49141 gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata
    49201 cgttcatatc agtgaacaac gccccggact gaaataacat gctcatccgt ttacgcactg
    49261 tatacaggcg cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat
    49321 gatctggtgc gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg
    49381 gccccatgat cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc
    49441 agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct
    49501 ccataattca cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga
    49561 acccaacata tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac
    49621 tttttagccg cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg
    49681 gaccggcgag tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt
    49741 gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt
    49801 tgccgaacct ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca
    49861 tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc
    49921 gttggtacag ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg
    49981 ctggttcgtc cttttaccgt ccattccgat gttctgcgcc gtgaattacc cttaatgttg
    50041 ttggtcagcg tggtggccgg ttccgtactc tatgacggac aacttagtcg aagcgatggt
    50101 atctttctcc tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag
    50161 gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc
    50221 gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg
    50281 gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac gatggctctg
    50341 acggcaattg ctatcggaac cagtctgccg gaactggcaa ccgcaatagc gggggttcgc
    50401 aaaggtgaaa acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc
    50461 atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccact ggcgtacagt
    50521 cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc
    50581 tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg
    50641 gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca
    50701 cgtagagtta caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga
    50761 acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga
    50821 aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat
    50881 cgggcgcaaa atggccgcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc
    50941 tagtgaagcc gcgcatggtg atttaggcat ggtcacccca caggatgtgg tgattgctat
    51001 ctctaactca ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta agcgtcttca
    51061 cgtaccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt
    51121 gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag
    51181 caccaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg
    51241 ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct
    51301 gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc
    51361 cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg
    51421 cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga
    51481 tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg
    51541 tgtgcgccct ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac
    51601 ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact
    51661 gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg
    51721 cttgcgacct gttacggccc tgtcagcgcc gacgttatgg caaaagcaga gaacattcgt
    51781 ctgctgatcc tcgacgttga tggcgtactg tcagatggcc tgatttatat gggcaataac
    51841 ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc
    51901 tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt
    51961 gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc
    52021 gatttgctgg aaaaattggc gattgccccg gaaaatgtgg cttatgttgg cgatgatctc
    52081 atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca
    52141 ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcgc
    52201 gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg
    52261 atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga
    52321 tcggcattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc
    52381 ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct
    52441 atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc
    52501 agccggtact taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata
    52561 aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac
    52621 ttgtgccaga ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga
    52681 cgcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg
    52741 gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa
    52801 catcctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt
    52861 ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt
    52921 gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc
    52981 atcgtcaccc agggcaccat caaaattaat gccgacaaag tggtcgttac ccgtccgggc
    53041 ggcgaacaag gtaaagaagt gattgacgga tacggtaaac cggcaacgtt ctaccagatg
    53101 caggacaacg gtaaacccgt tgaaggtcac gcttcccaga tgcactacga actggcaaaa
    53161 gattttgtcg ttctgacggg taatgcttat ctgcagcagg tcgatagcaa cattaagggc
    53221 gataagatca cttacctggt gaaagagcag aaaatgcagg ctttcagcga caaaggcaag
    53281 cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca aaaacaacaa aggccagacc
    53341 ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca
    53401 aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa
    53461 attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta catggttgta
    53521 ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg
    53581 cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc
    53641 cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct
    53701 gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg
    53761 cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc
    53821 gcactggctg cgaatccgaa atttattctg ctcgacgaac cgtttgccgg ggttgacccg
    53881 atctcggtta tcgacattaa acgcatcatt gaacacctgc gcgacagcgg cctgggcgtg
    53941 ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc
    54001 agtcaggggc atttgatctc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt
    54061 aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt
    54121 agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac
    54181 tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac
    54241 ttcagcagga gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc
    54301 atgaagaaat cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc
    54361 tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca
    54421 ccgctggtac accatccggc accagcggtg actacattga cgacgagctg ccggtctacc
    54481 agggcgaaac gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt
    54541 tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat gaaaccggtt
    54601 atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg
    54661 acgaggttga agccgtcctt aagcggatcc aacggtttga cccggtcggt gtagcggcaa
    54721 aagatctgcg tgactgcctg ctaatccaac tctcccaatt cgataagacc acgccgtggc
    54781 tggaagaggc cagattgatc attagcgatc atctcgatct gttagccaat cacgacttcc
    54841 gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga
    54901 tccagtcgct cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca
    54961 ttccagatgt gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca
    55021 gcattccgcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaacg
    55081 atggtgacag ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc
    55141 tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag
    55201 ccttctttga gcaaggtgaa gaatatatga aaccgatggt actggccgat atcgcccagg
    55261 ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc
    55321 cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg
    55381 gcgaagcttc ctccacggcg attcgtgcac tggtgaagaa attaatcgcg gcggaaaacc
    55441 cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg
    55501 tggcacgccg cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc
    55561 gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga
    55621 aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagctaaatt tgccaaactt
    55681 gagcaatatt ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga gaaagtcacc
    55741 cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt
    55801 caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa
    55861 cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct
    55921 gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga
    55981 gtgggcaggt tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt
    56041 gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg
    56101 gaaatcatca gcgagctggc ggcgaaacaa cttagcctgc cgcctcaggt ggtttttgaa
    56161 gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg
    56221 catggcaaac ttgaagaaga tactctgcgt gccgttggcg ttttcgttca gctcgaaacg
    56281 cctatagctt tcgatgccat cgacaaccaa ccggtggact tactttttgc cctgctggtg
    56341 ccggcagacc aaactaaaac gcacctgcat actctgtcgc tggtggcgaa acgtctggcg
    56401 gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc
    56461 attacggata ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga
    56521 cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt
    56581 aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc
    56641 gtagtgttgt tacccgatct ggctcgaact ctggccgatc gcgagatttc tgccgccgtc
    56701 agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac
    56761 ctgcctgacg ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt
    56821 cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa
    56881 agtgctatcg acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt
    56941 gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac ccgtctgctg
    57001 ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc
    57061 cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg
    57121 aaactgcgtc caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca
    57181 gaagtacaca attttatcta ccagacgcga agctatcttg agctatggtt acctatgctg
    57241 gaaaccaaca accgtagcta cctgacggtc gccattggtt gtaccggcgg gaagcaccgt
    57301 tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag
    57361 tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca
    57421 caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt
    57481 ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggctgaagcc aacagcgtta
    57541 ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggtc
    57601 cacaggaaga ggaagcactg gccgccgtta tcgccctctt taattctggt tttgatgaag
    57661 attaatcttc atcactttga catacaactc ccttcaagct cccccgacaa taagaaaatc
    57721 acgtactgaa atcgttctca atcaacgtca tttgtacatt ttgtgcgctt ttcactttca
    57781 gaagaacctt aagaaaacct taagaggcat tgtttaggtt ttgtttaagt taatcgacca
    57841 tactggagat cgtcagaaaa tatttccagg agatggcatg attcgcttat ctgaacaaag
    57901 tcccctgggc accggaagac atcggaaatg ttatgcgcat ccggaagatg cccaacgctg
    57961 tatcaagatt gtctaccatc gtggtggcga taaagagatc cgccgtgagt taaagtacta
    58021 cgcgcatctt ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt
    58081 tgagacggat tgcggaaccg gttatgtcta cgatgtgatt gccgattttg acggcaagcc
    58141 ttccattacg ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact
    58201 gcgccagcta ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc
    58261 gctgaagccg cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt
    58321 ctgcgacaac attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg
    58381 tctgcgtaag caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc
    58441 cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc
    58501 ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa
    58561 aatccacgcc tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg
    58621 attaggtaat acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt
    58681 gtggaaataa cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat
    58741 attcaggtaa accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa
    58801 tccagcttcc agcccttttc gtacccagct acgcccatcc cataaaaaga gattttggcc
    58861 gtctgctgag aaatcgttga agcaccgcga atacggtttt cattgcgctc gttgtgcgcc
    58921 agggctttct caatggaagc gacatcaaag ccccagtgct caggaaattt ctgatcttct
    58981 gcggcaatca ccgccagtcc catccacggc gagatttgat ccatactgac ccagtcagaa
    59041 tgtgccacgt aacgaaaatt gccatgcagc caggcgctga cctgtcgctc gaccattacc
    59101 gctgagaagg gaacaggcgc aacgctaaac aacgcgatgc ccccgcccca gaaaacggcg
    59161 aggacaacca ttaaccgcaa aagaaaacgg cgaacgaaac taaacaccgt taagcggctt
    59221 ttactcattc agccagaacc agcacgcggg aaaccagctt atcaatgccg ctcgccgctt
    59281 ctgcaatgtt ctgcgccagc atatatgctg gggtggtgac aatcttattg tcttcatcaa
    59341 ccacgatatc atcgacagga cacggcacat gctccgcgcc catctcttcc agcacttctg
    59401 cggtatcgat atcagtaccg atggtcaaac gcagcgggaa atcgaaaatt ttcggcagca
    59461 tcgccggggc aatacacata aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca
    59521 gcgcctttaa ttcacggtca acggtgcatt cgctaccaag actggcaaaa ttgcttaaat
    59581 tcttcgccgc gccaaccccc cccggcacaa tcaacgcatc cagttcagcg gcatcggcct
    59641 gggccagagg acggatttca ccgcgcgtta ttcgtgccgc ttcaatcagc acattgcgcg
    59701 tttccgtcat cgcttcgcca gttaaatggt tgataacatc aacctgctgc ttatccggtg
    59761 caaagcagac cgcctgcgca ccgctgcgtg aaatagctaa caacgtcaac accgcttcat
    59821 gaatttcaga accgtcatag acgccgcatc cgctcagaat tacgccaatt ttcttcattg
    59881 tgatcatcct ttcgcaattg actgaaacac attaattttt ttaataaaaa tggtacgcat
    59941 cacacattta actgattcat gtaacaaatc atttaagttt tgctatctta actgcgtgcg
    60001 gcctgaaaaa cagtgctgtg cccttgtaac tcatcataat aatttacggc gcagccaaga
    60061 tttccctggt gttggcgcag tattcgcgca ccccggtcta gccggggtca ttttttagtg
    60121 gcttttgcca cccacgcttt cagcacttct acgtcgtgac gccactcttc tttcatctct
    60181 tcaatccatt caccgacgtt atcttcccag gcgggaaggt caggagactg aatttgctga
    60241 cccagctgtt gcagatggcg taaccccact gaccccgccg cacctttaat tttatgtcct
    60301 tcctcaacaa tgcctttttt atcctgcgcc gtcagattca actccagcac gctgacatag
    60361 cccggcatca tcttctcaaa caccgctaac ccgtcggtga tcagcttcgg tcctacaagt
    60421 tcgagatact gttccagcat gggaatatcg agcaatgctt ctgatttact gttctcttct
    60481 gtcgtcaccg tactctcctc atcatcctgg gtatcccaga atttcttgat catcgcggtt
    60541 agcgccggaa ccgaaagcgg cttactcagc acatcatcca ttccagcatt gaggtactct
    60601 tgtttgtctt tcagcacgtt agcggttaag gccaccagcg gcggtaaatc ctcgcgcgga
    60661 taacgcttcg tcagttcacg agagatatcc agcccggtca tatctggcaa ctgaatatcc
    60721 agcaacacca ggtcgtattc gcccggttta aacatctcca gcgccgcctt gccggtcatg
    60781 gcgacatcaa cgctgttacc taatttttcc agcacagaac gcgcgacaat cacgttcagt
    60841 tcaatgtctt ccaccagcag cacattcagc gccggtaaag gcatatcgtc ttcatcaaac
    60901 gcatcatcga cctcttctgc caccgacggt gcgtggatcg tcaacgtaaa ggttgaacct
    60961 ttgccctgtt cactggtaac cgtaatatcg ccgcccatat ttttcgccag acgacgagaa
    61021 acggccagac caataccggt gccggtggca ggtttaccgc catgactgtc tttcacctgg
    61081 taatacatgg cgaagatttt atccagctca tcctgcggaa tgccgatacc agaatcttcc
    61141 acttcaaaat gcagcatatc gccttcatcg tagcgcacgc gcacggtaac ctggccttgc
    61201 tgggtgaatt tgacggcgtt actgatgagg ttccacagga tctgccgtaa acgcgtcccg
    61261 tcggtaatga cctgatgcgg taatggcagc gtaggctcca ggttaaagcg caatcctttt
    61321 tgttgcgcct gcaatgcgga gagattttcc agatcggcaa ggaagctggt gaaatcaacc
    61381 ggctgattat caagctggac cttgcgccgt tccatcttat ccatgtcgat aatatcgtta
    61441 aagatattcc ccagcgtgac ggccgaaaca tggatagttt tgagatattt ttcctgctcg
    61501 gcggtgagtt cggtatccag cagaatgcgg ctcaggccga cgataccgtt cagcggtgta
    61561 cgcaattcgt gactgatggt ggaaataaac gtcgttttgt cgcggctggc gcgttcaagc
    61621 gcatcctgat accgcttacg ctcggtaatg tcgcgaccaa agcccatcaa accgtgacgt
    61681 ttacccacgc ggtcgtagta cggcacttta cggatttcaa agcaggcttt gcgcccgtcc
    61741 gggtaatcca gccactgttc ataggtcagt gacacattat gacggaacac tttttcatcg
    61801 gtttcaatga cttttgcggc ggcttccggt gagtaaacat cagcaggttt caggtgaacc
    61861 agttgttttt cgctttttcc ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct
    61921 ttatcttcgt tacgataaaa aaccaagtcg ggtgaagcat caaggaagga acgtaagaat
    61981 gaggattgct gctcgagctg aatttgtgtc tcttcgcgct ctttgatttc aattttcagt
    62041 tggccgaagg tttcctgcag ttctgcttcc gctttttcac gaacggcaat ttcctgattt
    62101 agctgggcaa tattatcttt taactgaacg ttgaggctca aatcgcgctc gcgcatctcc
    62161 tccagttttt gcaccagccg tgacagacgt tgtcgtgact cctccagttg ctcgacgacc
    62221 accgatagaa agtagaccgc ccacggcgta atcagcaaac caaagaagat agaacgaata
    62281 acatcaatgc tttcgacctg accatgcagc accatggtta ccgccatttg taccacaatg
    62341 gcaagaacga cgagggccag cgccagcaac attgagaagc gcaccagacc taacttcatc
    62401 atcaggtcaa cataatactg cgccagcaga cgaatttgct tcattaggga attccttcac
    62461 gacaacctgc cccaataata cccaattctg acagttacgt tataaattgt gtgagaaatg
    62521 cggaattccc ctcaacgacc tactccgttg gaggtagcca cggacgtccc agcgccgatc
    62581 cctgtacgcc atgttcattc agatagcgat ccagctcgac cattcccgtc catcgatttt
    62641 cgcaccacag cggagcaagc agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga
    62701 tcacttccgg tggcgtatgg cgaatcattt ctccggcagt gagcgtataa tcctccagtt
    62761 caataccgtt caaacgcacc gcttcccagg ctttcgccat aatgctgcct ttcacaatat
    62821 gcagcggatg cagctttatg ccgtccacgc tggtttcaac cactcgctcc agcgtttgca
    62881 aacattcagc ctgcccttcg ccaggcaggc cgacaattaa gtgggaacac accttcagcc
    62941 cgcgctgacg tgccagctgg gttgtacgct gataacaggc aaaatcatgg ccgcggttga
    63001 tgcgatgcag tgttttgtcg tgggcggttt gtagccccag ctccagccac acttcgtaac
    63061 cctggtcctt atattcgcaa agcagatcca gcaccgcatc cggcacgcag tccgggcggg
    63121 taccaacaca caaaccgaca atattggcct ggctcaccgc ctgctgatac atagaacgca
    63181 gcacctgaac ttccgcaaag gtgctggtat acgcctgaaa gtaggccaga tagcgtttag
    63241 cgcggttaac taaattcgcc tggtgcgcca gttgctcggc aatggaacga tgctgctgcg
    63301 cttcatcggc aaacgaggca acattacaga atgtgcagcc accacgcccg atggtaccgt
    63361 cacggttagg gcagctaaaa ccgccgtgca gcgtcagctt atgcaccttt tgcccataac
    63421 gacgggtgag atcaccacca aacatattga ctaatttctg taactgcata atctgataga
    63481 ccgcgccttg aaaagaggcc aaagcctgcc atttttagct caattcggcg atgacctggg
    63541 tcaatcgtcc cggcctgctt ttatcaactg cataatcaat caaaattacc gaaatttcat
    63601 gcacaatcac ataaatcact tttgcttatc ttgtgtcaga tttttttatc tccttatgga
    63661 ttttaggcaa aaacagtagc atgaaacgtc attaccaatt aaggcagtat aaaatgctgg
    63721 ttttctcgtc agttcaaggc aggataaggg ttaacacgcc tttatgacag tcaggaattg
    63781 actgtttctc taacgactct ccttttagcc ttaaagataa aatctatttt aatttcagct
    63841 atttaataaa gaattttgcg ctaaagcaca tttctgtacc aataagcttg ccatttgacc
    63901 tgtatcagct ttcccgataa gttggaaatc cgctggaagc tttctggatg agcagcctgc
    63961 tcatcatatt tatgcagtaa ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg
    64021 acaacaaggg ggcgaatgcg aggcgcgcgt atgacacgca aaccccgtcg ccacgctctt
    64081 tctgtgcccg tgcgcagcgg ttcggaagtg gggttcccgc agagcctggg ggaggttcac
    64141 gatatgttgt acgataaatc ccttgagagg gataactgtg gtttcggcct gatcgcccac
    64201 atagaaggcg aacctagcca caaggtagtg cgtactgcaa tacacgcact ggcccgcatg
    64261 cagcaccgtg gcgcgattct cgccgatggt aaaaccggcg acggttgcgg cttgctgtta
    64321 caaaaaccgg atcgcttttt tcgcatcgtt gcgcaggagc gcggctggcg tttagcaaaa
    64381 aactacgctg tcgggatgct cttcctgaat aaagatcctg aactcgccgc tgccgcacgc
    64441 cgcatcgttg aagaagaact gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc
    64501 cccactaacg aaggcgtgct gggtgaaatc gccctctcct ctctgccacg cattgagcaa
    64561 atttttgtga acgccccggc aggctggcgt ccacgcgata tggagcgccg tctgtttatc
    64621 gcccgccgcc gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt ctgtagcctg
    64681 tcgaatctgg tgaacatcta taaaggtctg tgtatgccgg cggatctgcc gcgcttttat
    64741 ctggatcttg cggacctgcg tctggaatcg gccatttgcc tgttccacca gcgcttctcc
    64801 actaacaccg taccgcgctg gccgctggcg caaccgttcc gctatctggc gcataacggt
    64861 gaaatcaaca ccatcaccgg taaccgccaa tgggcgcgtg cgcgtaccta taaattccag
    64921 acgccgctga tccctgacct gcacgacgcc gcgccattcg tcaacgaaac cggttctgac
    64981 tccagttcca tggataacat gctggaactg ctgctggcag gcgggatgga tatcatccgc
    65041 gccatgcgcc tgttagtgcc acccgcctgg cagaacaacc cggatatgga cccggaactg
    65101 cgtgccttct ttgactttaa ctccatgcat atggagccgt gggatggtcc ggcgggcatc
    65161 gtgatgtccg atggtcgctt cgctgcctgt aacctcgacc gtaacggtct gcgtccggca
    65221 cgctacgtca ttaccaaaga caagctcatc acctgcgcct ctgaagtcgg tatctgggat
    65281 taccagcctg acgaagtggt cgaaaaaggc cgcgtcgggc caggcgaact gatggtgatc
    65341 gacactcgca gtgggcgtat tctgcactcg gcagaaaccg atgacgatct gaaaagccgc
    65401 cacccttata aagagtggat ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg
    65461 cccgatgaag aagtgggtag ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa
    65521 cagtttaact acagcgcgga agagctggac tccgtaattc gcgtactggg cgaaaacggt
    65581 caggaagcgg tcggttcgat gggcgatgat accccattcg ccgtgctctc cagtcagccg
    65641 cgcattattt acgactactt ccgccagcag tttgcccagg tgactaaccc gccaatcgac
    65701 ccgctgcgtg aagcgcatgt tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc
    65761 ttttgcgaag cagagggcca ggcgcaccgt ttaagcttta aatcgccgat tctgctctac
    65821 tccgatttca aacagctcac gacgatgaaa gaggagcact accgcgcaga tacgctggat
    65881 atcacctttg acgtcactaa aaccacgctc gaagcgacag tcaaagagct atgcgacaaa
    65941 gccgaaaaaa tggtacgtag cggcaccgtg ttgctggtgc tctccgaccg gaatatcgct
    66001 aaagatcgcc tgccggttcc ggctccgatg gcggttggcg cgatccagac ccgtctggtc
    66061 gatcaaagcc tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg
    66121 caccacttcg ccgtgttgct gggtttcggc gcgacggcta tttatccgta ccttgcctat
    66181 gaaacgctgg gccgcctggt agacacccac gcgattgcca aagattatcg taccgtgatg
    66241 ctcaactacc gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc
    66301 tccaccatcg cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct acacgatgat
    66361 gtagtgggcc tgtgcttcca gggcgcggtc agccgcattg gtggggcaag ctttgaagac
    66421 ttccagcagg atctgctgaa tctgtcgaaa cgtgcctggc tggcgcgcaa gccgattagc
    66481 cagggcggcc tgctgaaata cgtccacggc ggcgaatacc acgcctacaa cccggacgtg
    66541 gtgcgcacgc tgcaacaggc ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg
    66601 aagctggtta atgagcgtcc ggcaaccacg ctgcgcgatc tgctggcaat tacgccgggt
    66661 gaaaacgcgg tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa acgctttgat
    66721 accgccgcga tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg
    66781 atgaatagca tcggcggtaa ctcgaactcc ggtgaaggcg gcgaagaccc ggcgcgctat
    66841 ggcactaaca aagtgtcgcg catcaagcag gtggcttctg gtcgcttcgg cgttactccg
    66901 gcgtatctgg tcaatgccga cgtcattcag attaaagtcg cccagggcgc gaagccgggc
    66961 gaaggtggtc agttgccggg cgacaaagtc actccttaca tcgccaaact gcgctattcg
    67021 gtgcccggag tgacgctgat ctccccgccg ccgcaccacg atatctactc tatcgaagac
    67081 ttagcgcagc ttattttcga cctcaagcag gttaacccga aagcgatgat ctccgttaag
    67141 ctggtgtccg aaccaggcgt tggcaccatc gcgactggcg tggcgaaagc ttatgcggac
    67201 ttgatcacca tcgcgggcta tgacggcggc accggcgcaa gcccgctgtc gtcagtgaaa
    67261 tatgcaggct gcccgtggga actcggtctg gtggaaaccc aacaggcgct ggttgccaac
    67321 ggcttgcgtc ataaaattcg tttgcaggtt gatggcggcc tgaaaacggg tgtcgatatc
    67381 atcaaagcgg cgattctcgg cgcagaaagc ttcggcttcg gcactggccc aatggtggcg
    67441 ctcggctgta aatatctgcg tatttgccac ctgaacaact gcgccacggg tgtagcaact
    67501 caggatgaca aactgcgtaa gaatcactat cacggcctgc cgttcaaggt gactaattac
    67561 tttgagttta tcgcccgtga aacccgcgag ctgatggcac agctgggagt aacgcgtctg
    67621 gtggatctga ttggtcgcac cgacctgctg aaagagctgg acggtttcac cgccaaacag
    67681 cagaaactgg cgctgtcgaa gctgctggag actgccgagc cacatgcagg taagtcactc
    67741 tactgcactg aaaacaaccc gccgtttgat aacggcctac tgaacgcgca gttgctgcaa
    67801 caggcgaaac cgtttgtcga tgaacgccag agcaaaacct tctggttcga tatccgcaac
    67861 accgaccgtt ctgtcggcgc gtcgctttca ggctatatcg cccagacgca cggcgatcag
    67921 gggctggcag ccgatcctat caaagcgtac ttcaacggca ccgcaggcca gagtttcggt
    67981 gtgtggaacg cgggcggcgt ggaactgtac ctgaccgggg atgccaacga ctatgtcggt
    68041 aaaggcatgg cgggcggctt aatcgccatt cgtcctccgg ttggttccgc cttccgcagc
    68101 catgaagcaa gcattatcgg caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat
    68161 gccgcaggcc gcgcgggtga acgtttcggc gtgcggaact ccggtgctat caccgtggta
    68221 gaaggcattg gcgacaacgg ctgtgaatat atgacgggcg gtattgtctg tattctgggt
    68281 aaaaccggcg ttaacttcgg cgcgggcatg accggcggct tcgcttacgt tctcgatgaa
    68341 agcggtgatt tccgcaaacg cgttaacccg gaactggtcg aagtcttaag cgtcgacgat
    68401 ctggcgatcc atgaagagca tctgcgcggt cttatcaccg agcatgttca gcataccggt
    68461 tctcagcgcg gtgaagagat tctggcgaac tggtcaacct tcgccacgaa attcgcgctg
    68521 gttaaaccga agtccagtga tgtaaaagca ctgctgggtc accgtagtcg tagcgcagca
    68581 gagttgcgcg tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt ttatcaattt
    68641 atcgacctgc agcgcgttga tccgccaaag aaaccgctga agatccgtaa aattgagttt
    68701 gttgaaattt acgagccgtt ttccgaaggc caggccaaag cgcaggctga ccgctgtctg
    68761 tcgtgcggca acccatactg cgagtggaaa tgcccggtac acaactacat cccaaactgg
    68821 ctgaagctcg ccaacgaggg gcgtattttt gaagcggcag aactgtcgca tcagaccaac
    68881 acactgccgg aagtatgcgg acgtgtctgc ccgcaagacc gtctgtgcga aggttcctgc
    68941 accctgaacg atgagttcgg tgcggtgacc atcggcaaca ttgagcgtta tatcaatgat
    69001 aaagcgttcg aaatgggctg gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa
    69061 gtggcgatta tcggtgcagg cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac
    69121 ggcgtaaaag ccgttgtctt cgaccgtcat ccagaaattg gcgggctgct gaccttcggt
    69181 attccggcct tcaagctgga aaaagaggta atgacgcgtc gccgtgaaat cttcaccggc
    69241 atgggtattg aattcaaact caataccgaa gtgggccgcg acgtacagct ggacgatctg
    69301 ctgagtgatt acgatgccgt gttccttggc gttgggactt atcagtcaat gcgcggcggg
    69361 ctggaaaacg aagacgccga tggcgtgtac gcagcgctgc cgttccttat cgccaacacc
    69421 aaacagttaa tgggctttgg tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa
    69481 cgcgtggtag tacttggcgg tggcgacact gcgatggact gcgtgcgcac gtctgtacgc
    69541 cagggggcaa agcacgttac ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc
    69601 cgccgcgaag tgaaaaacgc gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca
    69661 ctggggattg aagtgaacgg taacggcaaa gtcagcggcg taaaaatggt acgtaccgaa
    69721 atgggcgaac cggatgccaa aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat
    69781 atcgttccgg cagatgcggt gatcatggcg tttggtttcc gtccacacaa catggaatgg
    69841 ctggcaaaac acagcgtcga gctggattca caaggccgca tcatcgctcc ggaaggcaac
    69901 gacaacgctt tccagaccag caacccgaaa atctttgctg gcggcgatat cgtccgtggt
    69961 tccgatctgg tcgttaccgc catcgccgaa ggtcgcaaag cggcagacgg cattatgaac
    70021 tggctggaag tttaagctct gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa
    70081 ttctcctttc tttctggaaa tcgcctcgca taatctgaag aaaaccaatg taatacgctc
    70141 atctggattg ttaccggtag atgcccggcg tatagctgat tccattattc aacgcagcgc
    70201 catataaagg gacagataat ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa
    70261 atccacaacg gcattattca tctggtcgat agcgcccgaa cggaaacggt acgtagcgtt
    70321 aacgcgctga tgaccgcgac gtactgggaa attggccgac ggattgtcga atttgaacaa
    70381 ggtggtgagg ccagagcagc ttatggtgca cagttgattg atcggctatc gaaggattta
    70441 agtcgacggt ataaacgtgg ggtctctgca aaaaacttac gccaaatgag gcttttttac
    70501 ctcttttttc aacatgttga aattcgccag acagtgtctg gcgaattaac accattggga
    70561 attccgcaga caccatctgc agaattccca tcggcaaaga tttggcagac gctgtctgcc
    70621 aaattgagag gctgtttgat atttccacag cttttgtatt gcatctccta ttttcggttt
    70681 gtatattcat gcaaataaac gaag
//
LOCUS       DAEEGA010000007        69409 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEEGA010000007 DAEEGA010000000
VERSION     DAEEGA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 69409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69409
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.007"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..255)
                     /locus_tag="KLH46_000468"
     CDS             complement(<1..255)
                     /locus_tag="KLH46_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBE0377287.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIV"
     gene            complement(610..2781)
                     /gene="glcB"
                     /locus_tag="KLH46_000469"
     CDS             complement(610..2781)
                     /gene="glcB"
                     /locus_tag="KLH46_000469"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBE0377288.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(2803..3207)
                     /locus_tag="KLH46_000470"
     CDS             complement(2803..3207)
                     /locus_tag="KLH46_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HBE0377289.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(3212..4431)
                     /gene="glcF"
                     /locus_tag="KLH46_000471"
                     /pseudo
     CDS             complement(3212..4431)
                     /gene="glcF"
                     /locus_tag="KLH46_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(4442..5494)
                     /gene="glcE"
                     /locus_tag="KLH46_000472"
     CDS             complement(4442..5494)
                     /gene="glcE"
                     /locus_tag="KLH46_000472"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBE0377290.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(5494..6993)
                     /gene="glcD"
                     /locus_tag="KLH46_000473"
                     /pseudo
     CDS             complement(5494..6993)
                     /gene="glcD"
                     /locus_tag="KLH46_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            7244..8014
                     /gene="glcC"
                     /locus_tag="KLH46_000474"
     CDS             7244..8014
                     /gene="glcC"
                     /locus_tag="KLH46_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HBE0377291.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(8138..9279)
                     /locus_tag="KLH46_000475"
                     /pseudo
     CDS             complement(8138..9279)
                     /locus_tag="KLH46_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            9641..11371
                     /locus_tag="KLH46_000476"
     CDS             9641..11371
                     /locus_tag="KLH46_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HBE0377292.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            11368..12282
                     /locus_tag="KLH46_000477"
     CDS             11368..12282
                     /locus_tag="KLH46_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBE0377293.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            12315..12563
                     /locus_tag="KLH46_000478"
     CDS             12315..12563
                     /locus_tag="KLH46_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBE0377294.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            12563..13734
                     /locus_tag="KLH46_000479"
                     /pseudo
     CDS             12563..13734
                     /locus_tag="KLH46_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(13770..14849)
                     /gene="lptG"
                     /locus_tag="KLH46_000480"
     CDS             complement(13770..14849)
                     /gene="lptG"
                     /locus_tag="KLH46_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBE0377295.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(14846..15916)
                     /gene="lptF"
                     /locus_tag="KLH46_000481"
     CDS             complement(14846..15916)
                     /gene="lptF"
                     /locus_tag="KLH46_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBE0377296.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(15947..16507)
                     /locus_tag="KLH46_000482"
     CDS             complement(15947..16507)
                     /locus_tag="KLH46_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBE0377297.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(16519..17343)
                     /locus_tag="KLH46_000483"
     CDS             complement(16519..17343)
                     /locus_tag="KLH46_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377298.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(17343..18692)
                     /locus_tag="KLH46_000484"
     CDS             complement(17343..18692)
                     /locus_tag="KLH46_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBE0377299.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(18738..19496)
                     /locus_tag="KLH46_000485"
     CDS             complement(18738..19496)
                     /locus_tag="KLH46_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377300.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(19528..20241)
                     /locus_tag="KLH46_000486"
     CDS             complement(19528..20241)
                     /locus_tag="KLH46_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBE0377301.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            20415..21107
                     /locus_tag="KLH46_000487"
     CDS             20415..21107
                     /locus_tag="KLH46_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBE0377302.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(21156..22655)
                     /gene="pitB"
                     /locus_tag="KLH46_000488"
     CDS             complement(21156..22655)
                     /gene="pitB"
                     /locus_tag="KLH46_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBE0377303.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(22947..24806)
                     /gene="gss"
                     /locus_tag="KLH46_000489"
     CDS             complement(22947..24806)
                     /gene="gss"
                     /locus_tag="KLH46_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBE0377304.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            25011..25877
                     /gene="yghU"
                     /locus_tag="KLH46_000490"
     CDS             25011..25877
                     /gene="yghU"
                     /locus_tag="KLH46_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBE0377305.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(26000..26248)
                     /gene="hybG"
                     /locus_tag="KLH46_000491"
     CDS             complement(26000..26248)
                     /gene="hybG"
                     /locus_tag="KLH46_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBE0377306.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(26382..26723)
                     /gene="hypA"
                     /locus_tag="KLH46_000492"
     CDS             complement(26382..26723)
                     /gene="hypA"
                     /locus_tag="KLH46_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBE0377307.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(26716..27204)
                     /gene="hybE"
                     /locus_tag="KLH46_000493"
     CDS             complement(26716..27204)
                     /gene="hybE"
                     /locus_tag="KLH46_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBE0377308.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRSE
                     NA"
     gene            complement(27197..27691)
                     /locus_tag="KLH46_000494"
     CDS             complement(27197..27691)
                     /locus_tag="KLH46_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBE0377309.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(27691..29394)
                     /gene="hybC"
                     /locus_tag="KLH46_000495"
     CDS             complement(27691..29394)
                     /gene="hybC"
                     /locus_tag="KLH46_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBE0377310.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(29391..30569)
                     /gene="hybB"
                     /locus_tag="KLH46_000496"
     CDS             complement(29391..30569)
                     /gene="hybB"
                     /locus_tag="KLH46_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBE0377311.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(30559..31545)
                     /gene="hybA"
                     /locus_tag="KLH46_000497"
     CDS             complement(30559..31545)
                     /gene="hybA"
                     /locus_tag="KLH46_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBE0377312.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(31548..32666)
                     /gene="hybO"
                     /locus_tag="KLH46_000498"
     CDS             complement(31548..32666)
                     /gene="hybO"
                     /locus_tag="KLH46_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBE0377313.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(32855..33142)
                     /locus_tag="KLH46_000499"
     CDS             complement(32855..33142)
                     /locus_tag="KLH46_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBE0377314.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(33261..34148)
                     /locus_tag="KLH46_000500"
     CDS             complement(33261..34148)
                     /locus_tag="KLH46_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBE0377315.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            34305..35345
                     /gene="gpr"
                     /locus_tag="KLH46_000501"
     CDS             34305..35345
                     /gene="gpr"
                     /locus_tag="KLH46_000501"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBE0377316.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(35385..35879)
                     /locus_tag="KLH46_000502"
     CDS             complement(35385..35879)
                     /locus_tag="KLH46_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBE0377317.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            36070..36954
                     /gene="yghA"
                     /locus_tag="KLH46_000503"
     CDS             36070..36954
                     /gene="yghA"
                     /locus_tag="KLH46_000503"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBE0377318.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(37226..37651)
                     /gene="exbD"
                     /locus_tag="KLH46_000504"
     CDS             complement(37226..37651)
                     /gene="exbD"
                     /locus_tag="KLH46_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBE0377319.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(37658..38392)
                     /gene="exbB"
                     /locus_tag="KLH46_000505"
     CDS             complement(37658..38392)
                     /gene="exbB"
                     /locus_tag="KLH46_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBE0377320.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            38644..39831
                     /gene="metC"
                     /locus_tag="KLH46_000506"
     CDS             38644..39831
                     /gene="metC"
                     /locus_tag="KLH46_000506"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBE0377321.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            39971..40630
                     /locus_tag="KLH46_000507"
     CDS             39971..40630
                     /locus_tag="KLH46_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0377322.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(40670..41569)
                     /gene="yqhC"
                     /locus_tag="KLH46_000508"
     CDS             complement(40670..41569)
                     /gene="yqhC"
                     /locus_tag="KLH46_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBE0377323.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            41763..42926
                     /gene="yqhD"
                     /locus_tag="KLH46_000509"
     CDS             41763..42926
                     /gene="yqhD"
                     /locus_tag="KLH46_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBE0377324.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            43031..43858
                     /gene="dkgA"
                     /locus_tag="KLH46_000510"
     CDS             43031..43858
                     /gene="dkgA"
                     /locus_tag="KLH46_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBE0377325.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            44058..44984
                     /locus_tag="KLH46_000511"
     CDS             44058..44984
                     /locus_tag="KLH46_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBE0377326.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            45035..45292
                     /gene="yqhH"
                     /locus_tag="KLH46_000512"
     CDS             45035..45292
                     /gene="yqhH"
                     /locus_tag="KLH46_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBE0377327.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(45335..47554)
                     /locus_tag="KLH46_000513"
     CDS             complement(45335..47554)
                     /locus_tag="KLH46_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBE0377328.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVVHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIAPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(47665..49077)
                     /gene="ftsP"
                     /locus_tag="KLH46_000514"
     CDS             complement(47665..49077)
                     /gene="ftsP"
                     /locus_tag="KLH46_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBE0377329.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(49152..49889)
                     /gene="plsC"
                     /locus_tag="KLH46_000515"
                     /gene_synonym="parF"
     CDS             complement(49152..49889)
                     /gene="plsC"
                     /locus_tag="KLH46_000515"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBE0377330.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(50123..52381)
                     /gene="parC"
                     /locus_tag="KLH46_000516"
     CDS             complement(50123..52381)
                     /gene="parC"
                     /locus_tag="KLH46_000516"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBE0377331.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(52927..53409)
                     /locus_tag="KLH46_000517"
     CDS             complement(52927..53409)
                     /locus_tag="KLH46_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBE0377332.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(53462..53854)
                     /gene="ygiW"
                     /locus_tag="KLH46_000518"
     CDS             complement(53462..53854)
                     /gene="ygiW"
                     /locus_tag="KLH46_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBE0377333.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            54006..54665
                     /gene="qseB"
                     /locus_tag="KLH46_000519"
     CDS             54006..54665
                     /gene="qseB"
                     /locus_tag="KLH46_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBE0377334.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAAKLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            54662..55936
                     /gene="qseC"
                     /locus_tag="KLH46_000520"
     CDS             54662..55936
                     /gene="qseC"
                     /locus_tag="KLH46_000520"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBE0377335.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(55982..56314)
                     /locus_tag="KLH46_000521"
     CDS             complement(55982..56314)
                     /locus_tag="KLH46_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HBE0377336.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            56633..57214
                     /gene="mdaB"
                     /locus_tag="KLH46_000522"
     CDS             56633..57214
                     /gene="mdaB"
                     /locus_tag="KLH46_000522"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBE0377337.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            57245..57559
                     /locus_tag="KLH46_000523"
     CDS             57245..57559
                     /locus_tag="KLH46_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBE0377338.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(57607..59499)
                     /gene="parE"
                     /locus_tag="KLH46_000524"
     CDS             complement(57607..59499)
                     /gene="parE"
                     /locus_tag="KLH46_000524"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBE0377339.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(59528..60109)
                     /gene="yqiA"
                     /locus_tag="KLH46_000525"
     CDS             complement(59528..60109)
                     /gene="yqiA"
                     /locus_tag="KLH46_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBE0377340.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(60109..60936)
                     /gene="cpdA"
                     /locus_tag="KLH46_000526"
     CDS             complement(60109..60936)
                     /gene="cpdA"
                     /locus_tag="KLH46_000526"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBE0377341.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(60961..61383)
                     /locus_tag="KLH46_000527"
     CDS             complement(60961..61383)
                     /locus_tag="KLH46_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBE0377342.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(61384..62013)
                     /gene="nudF"
                     /locus_tag="KLH46_000528"
     CDS             complement(61384..62013)
                     /gene="nudF"
                     /locus_tag="KLH46_000528"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBE0377343.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            62218..63699
                     /gene="tolC"
                     /locus_tag="KLH46_000529"
     CDS             62218..63699
                     /gene="tolC"
                     /locus_tag="KLH46_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBE0377344.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            63847..64518
                     /locus_tag="KLH46_000530"
     CDS             63847..64518
                     /locus_tag="KLH46_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBE0377345.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            64524..65684
                     /locus_tag="KLH46_000531"
     CDS             64524..65684
                     /locus_tag="KLH46_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBE0377346.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(65722..66510)
                     /gene="ygiD"
                     /locus_tag="KLH46_000532"
     CDS             complement(65722..66510)
                     /gene="ygiD"
                     /locus_tag="KLH46_000532"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBE0377347.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPVAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            66653..67426
                     /gene="zupT"
                     /locus_tag="KLH46_000533"
     CDS             66653..67426
                     /gene="zupT"
                     /locus_tag="KLH46_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBE0377348.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(67484..67654)
                     /locus_tag="KLH46_000534"
     CDS             complement(67484..67654)
                     /locus_tag="KLH46_000534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377349.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(67915..68568)
                     /gene="ribB"
                     /locus_tag="KLH46_000535"
     CDS             complement(67915..68568)
                     /gene="ribB"
                     /locus_tag="KLH46_000535"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBE0377350.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(68672..68820)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            68942..69232
                     /gene="ubiK"
                     /locus_tag="KLH46_000536"
     CDS             68942..69232
                     /gene="ubiK"
                     /locus_tag="KLH46_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBE0377351.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        17194 a        18546 c        17207 g        16462 t
ORIGIN      
        1 gacaataatc cacgcaattg gccataatcc ataaataaag ccatagcccg cagcagcaaa
       61 tgccatatca atcggcattt taaaggcgaa tatcgcaatc aggatagata atataagggt
      121 tattgctcca gcgacatgtc ctttcagacg taataccgcg agtgcaacga agaagaatat
      181 tatcgggatc agggcgacca gagcggatag ccccagtcct cccatcggca tatacatttg
      241 ggtccaggta accatattgt tcagtctctt attatcttta tatgcttgat atacttaagg
      301 ttgtaataag caaaagagga ctgaactgta aaatataggc gttatacttt acagcaacag
      361 tacgccgcta acgcaattgc tacctctggc ataacaagta tatcgggtaa gggtttctgt
      421 tccgcacacg caggcgcaga gtatcgttaa gatgtccata ttgttgtttt aggcccgcta
      481 gtaatgcgct acgggtattt aatattgtta aaccctgata atcgctccgg ttatttccgg
      541 gataaatgta ctaccgcagt tactatcata gccccgacaa taaaacttgc cggggctttt
      601 ttgacgctat taatgacttt ctttttcgcg taaacgccag gcgtgtaata acggttcggt
      661 ataaccgttt ggctgtttca cgccgaggaa gattaaatcg ctggcagctt taaaagcaca
      721 cgagttagcg aaattccccg ccatcggacg ataagccgga tcgccagcat tttgctgatc
      781 aaccactttc gccatattct ccagcgacgc ctgcacctgt tctttggtca gaataccgtg
      841 acgtaaccag ttggcgatat gctggctgga gatacgcagc gttgcgcggt cttccatcag
      901 cgccacgttg tgaatatccg gcacttttga acaaccaatc ccctgctcca cccaacgcac
      961 cacgtacccc agaatcccct gcacgttgtt atccagctct tgttggatct cttgcgccga
     1021 ccagttagcg ttttcagcca ccggaatagt cagcagatcg tccagcagcg gttcaaattc
     1081 agcactgaac tcggtctggg caatgttggc ttgcacgctc tgtacgttgg tatggtggta
     1141 gtgcagcgca tggagcgtag cagcggttgg tgacggaacc caggctgtgt ttgccccggc
     1201 acgcagttgg tcgcccttct ggctgtacat gtctgccatc aggtccggca ttgcccacat
     1261 gcctttaccg atttgcgctt taccgcgcag cccacagaac agaccggaaa gcacgttatt
     1321 acgttcgtag gctttgatcc acggcgtcga tttcatctgg tttttacgca gcatcgggcc
     1381 agcttccatc accgaatgca tttcatcgcc ggtacggtcg aggaaaccgg tattgatgaa
     1441 cgccacgcgg ttgcgcgcct gagcgataca gctacgcaag ttcagcgagg tccggcgttc
     1501 ttcatccata atgcccattt tcagggtatt cggtgccata ccgagcattg tctcaatgcg
     1561 ggtaaacagt ttgttggcga acgccacttc ctgcggaccg tgcattttcg gtttcacaat
     1621 atagacgctg ccagtgcgcg agtttttctg cacttttaaa tcatagaggg caatcgcgcc
     1681 agtcatgacg ccatcaagaa taccttccgg gatttcattg ccttcgctgt cccaaatcac
     1741 aggaatggtc atcaaatgac ccacgttgcg gataaacagc aacgagcgtc cgtgcagaga
     1801 aatttcagag ccatcggcgg cggtgtactg acgatcgtca ttcagtttac gtacgatttg
     1861 ccgaccgttt ttctccattt tctcttgcag agtcccctgc atcaggccca gcagattgcg
     1921 gtacagcagg attttatctt ccgcatcaac cgccgcgacc gaatcttcgc agtcgagaat
     1981 ggtactgata gcagcttcga cgataacatc gttgatgtgc gccggatcgt ctttgccaat
     2041 ccgcccgttg gcatcaattt gcagttcaat atgcaggccg ttatttttca gcaaaatgca
     2101 ggtcggcgca gcggcatcgc cacggtaacc gacaaactgt gctggagtac gtaacgtggt
     2161 ttctttacca tttttcaact ggatgcgtaa ttgtttatcg accaccttaa acgccaccac
     2221 atcctgatag ctgccgtttt ccagcggtag tgattcatcg aggaaacgtc gaacccaggc
     2281 gataacctgc tcaccgcgct gcggatcgta gccactgacc atcgcccctt cctgcgggat
     2341 gatgtcgctg ccgtataacg catcgtacag tgagccccag cgagcgttcg ccgcgttcag
     2401 cgcgtagcgg gcgttcattg ccggaaccac cagctgtggc cccgcctgac tggtgatttc
     2461 gctgtcaatg cccgtggttt ccaccgtcac gcgctccggt tgcggcacca ggtagcccag
     2521 ttcacgaagg aaagatttat aggccgcttt atcttttacc ggccccggat tgctgcgatg
     2581 ccactcatca agcgccgcct gaatgcgatc gcgttctgcc agcaactgac gattttctgg
     2641 tgccagatcg tgaacgatct catcaacatt gtgccagaat gcagcagcgt ccagttccac
     2701 gcccggcaaa acttctccat ccacaaaacg tttaaaattg gcgtcaatgc gtaaacggcc
     2761 ctgggttatg gtttgactca ttgtttctct cctcgttttc gcttatttcg ccaacaccgc
     2821 tgctgccact ttcgcgacct gcgcatcctg tgctccggtt aaaccagaaa cgcccacggc
     2881 accaataatt tgcccatcaa caacaaccgg tacgccgcct tccagcgacg tcagtaacgg
     2941 cgcagtcacg aacgcggtac gtccgttgtt caccatctct tcatagccct tagtttcacg
     3001 acgccccagc gcggcggtac gcgctttctc ctgggagatg taagccgcaa tcggcgcgca
     3061 atcgtccatg cgacttaacg ccagcagatg accgccgtca tcggcaacag caatggaaac
     3121 agaccagtta tttttctgcg cctcttcctg acctgcggca ataattgcac tcgccatttg
     3181 ctggctaaga atgactttag ttttcatttt tttattcctt ttcaagggct tgttctacaa
     3241 tttcaatcca gtgacgcaca gaggtacgac cggcgctcgc cagatgcgtc tggcaaccaa
     3301 tgttggcggt gacgatcatt tccggtttgc cgctttccag cgcattcatt ttgttatccc
     3361 gcagctggcg tgccagatcg ggatgcgtta acgcatatgt tcccgctgaa ccgcagcaca
     3421 gatggctgtc gggaacgtcc gttaaggtaa atccaagacg aagtaacact ttttccactt
     3481 cgccgttcag cttttgcgca tgttgtaggg tacacgggca gtggaaggcc agttttttat
     3541 cgccgcgaat tgccagtttt tccagcggtt cctcgcgcag aagttcgact aaatcgaccg
     3601 ccagttcact gacctgacgc gctttatcgg catataacgc atcgtttttc agcatctgcc
     3661 catactcttt gacgaacgcg ccgcaaccgc tggcggtttg caaaattgcc tcggcacctg
     3721 cttcaatcgc gggccaccag gcatcaatat tattgcgcgc ccgtgccaac cctttctcct
     3781 gcgcattaag atgatagtcc accgcgccac aacagcctgc ttcgttagct gacatgacgc
     3841 tgatccccag acgatccagc actcgcgcag ttgccgcgtt ggtgttgggc gaaagcgtag
     3901 gctgggcgca gccttccagc attaaaaccc gacgcttatg gcgcagcggc ggacgcggtt
     3961 tagctttcac cgtttcagca ggcagttttg ctctgacctg ttccggtaaa aacggtcgca
     4021 gcaccagccc tacctgcgtc agcgcacgga agaccgccgg acgcggcact acctggcgca
     4081 atccttcgcg cagcattcgc tccggcagtg ggcgtttcac tttctgctcg acaatatcac
     4141 gcccgatatc cagcaaattg tgatagcgca caccagaagg acaggtggtt tcacaattgc
     4201 ggcaagtgag gcagcgatcg agatgctcct gtgttttaag cgtgacttcg ttgccttcca
     4261 gcacctgttt aatcagatag atgcgcccgc gcggcccgtc cagttcatcg cccagaagct
     4321 gataggttgg gcaggttgcg gtacaaaatc cgcagtgaac acaggcgcac aggatgctgt
     4381 cggcttccag cgcgcgttct gccgcatctc ttcggttaat cgggtttgca tagcctgctc
     4441 ctcaaagttc cgcgtacatg cgaccggggt taaacacgcc gcaagggtcg agctgctgtt
     4501 taagctgctg gtgatagcgg aataaaggag ccgatagcgg ggcaaagcca ccatctccgg
     4561 cactaaagcg ggtcgcatga ccgccagcgt tgcgggcgat gcgatggatt tgattgtcat
     4621 cggctgtcga tttcagccag cgtaacgccc cgccccagtc gatcagttgc tcgccgggta
     4681 aatccatcat cggcgcatcg ctgggtaatg aaatgcgcca taaggtacct ggtaacgaga
     4741 agaacggcag ttgttgttca cgcaattgct gccagaactg acccgcaacc tcttcgccac
     4801 ccagcagttc acgcgctgct tttaccgatc cttcgccgcc ctcaaggcgg atccacaacg
     4861 cattgtcgaa gtaacataag ccactaatgg gtaatggctg gagttgccac tcggcgattt
     4921 cactcatggc ttcttgcagg ctgatttccc gacgcaggct cagagaggca cgcggtcgcg
     4981 gtaacacttt cattgagatt tcagtgagca cgccaagaca accatagctt ccggccatta
     5041 accgtgagag atcgtatccg gcaacgtttt tcatcacttc gccaccaaaa cgcagatgtt
     5101 ttccagtgcc ggtaatgatg cgcgtgccga ggacaaaatc gcggaccgaa ccgctccacg
     5161 ggcgacgcgg ccccgccagc ccgcaggcga ccatcccgcc ccaggtggct tcttcaccat
     5221 aatgcggcgg ctcgcaggga agcatttgcc cggcgctctc cagcgccgct tcaatcgcca
     5281 ccagcggcgt tccggcacga gcggttatca ccagctcggt cgggtcgtaa ttaacaatgc
     5341 cgcgatgaca acgcacatcc agcgtttgcc cggtgactgg gcgacctaaa aaggctttgc
     5401 tattgctgcc ctgaatcacc agcggcgttt tatcgctaat tgcctgattc acctgctcca
     5461 gcagcgcctg actgtaatca cactcgcgta gcatcaaaag cgctccagtt caggaaaagg
     5521 taaatgaccg tgatgcacat gcatggcacc aaattcagca cagcggtgta gcgtgggaat
     5581 gtttttccca gggttcagca aaccatcggg gtcaaacgcc gccttgaccg catggaaggt
     5641 cgtgatttca tcgctgttga actgggcgca catttgattg attttttctc gcccgatgcc
     5701 atgttcccca ctgatgctgc cgccaacttc aacgcagagt tcgaggatct tcccgcccag
     5761 ctcttccgcg cgggcaaatt caccaggttc gttggcatcg aaaaggatta acgggtgcat
     5821 gttgccatct ccggcatgaa agacgttggc aacacgtaaa tcatattgct gcgataaacg
     5881 ggcaatgcct tccagtacgc caggcagggc gcgacgcggg atggtgccat ccatgcagta
     5941 gtaatccggg gagatacgtc ctaccgccgg gaacgcattt ttgcgaccgg cccagaaacg
     6001 tacgcgctct gcttcgtcct gtgccaaacg gacgtcagtc gcgcccgctt tcaacaagat
     6061 gtagttaacc cgctcgcagt cttcctgtac gtcagactcc acgccgtcca gttcgcataa
     6121 caaaatcgct tcggcgtcga cgggataacc ggcatgaata aaatcttccg ccgcgcggat
     6181 cgacaggtta tccatcatct ccagcccgcc ggggataatg ccattggcga tgatgtcacc
     6241 aaccgcaagt ccggcttttt ctaccgagtc aaagctggct aacagaaccc gcgccacggg
     6301 cggcttcggc agcagtttta ccgtcacttc ggtggtcacg ccgagcatac cttccgatcc
     6361 ggtgaacagc gccagcaggt caaaaccagg tgaatccagc gcgtccgatc ccagcgtcag
     6421 cgcctcgccg tccagcgttt gcacttcaat tttcagcagg ttatgtacgg tcagaccata
     6481 tttcaggcag tggacgccac cggcattttc cgccacattg ccgccaattg aacaggcgat
     6541 ttgtgaggaa ggatccggcg cgtaatagag attatgcggc gcaacggcct aggagatcgc
     6601 caggttacat acgcccggct gcacgcgcgc gcggcgacca acggggttaa tgtcgaggct
     6661 ctctttaaag cgcgccatca ccaacaacac acctttttcc agcggcagcg cgccaccaga
     6721 aagcccggtg cctgcaccac gggtcaccac cggtacacgc aggcgatggc agacagccag
     6781 aatcgctgtc acctgttcca tttgcttagg cagaacaacc agtaatggac gcgtgcgata
     6841 cgcgctcaac ccgtcacact cgtaaggaat gatctcctca tcggtatgca ggatctcaag
     6901 tccagggaca tgctcacgca gtgccatcag taccgatgtg cggtcgacat cgggtaaagc
     6961 gccatcaaga cgctcttcgt acaagatgct catgagtagg cttcgctttg ttgtgttgtg
     7021 tggcagctga tttttgcgcg ctgcttctgt gaacagttat taagcgggct tttcgttttc
     7081 gtctatctct ttagctaccg gtcagaccat tttttttcca gctctgtgac cttgtcttgg
     7141 ttaacttaat gttaaattga tgtaacataa tcacttgcgt gatgtgcgtg ttttgcgagt
     7201 taagaacaga aaaattggtc ctacctgtgc acgaggtccg ggaatgaaag atgaacgtcg
     7261 ccctatttgc gaagtggttg cagagagtat cgaacggtta attatcgacg gcgtactgaa
     7321 ggtcggtcag ccgcttccct cggaacgtcg actgtgtgaa aagctcggct tctcacgctc
     7381 cgcactgcgt gaagggctga ccgtgctgcg cgggcgcggg attattgaaa cggcgcaggg
     7441 tcgcgattct catgtcgcac ggcttaatcg ggagcaggac accagcccgc tgatccatct
     7501 gttcagtacg cagccgcgaa cgctgtacga tctgctcgac gttcgcgcat tactggaggg
     7561 cgaatcggca aggctggcgg caacgctggg aacgcaggct gattttgttg tgataacccg
     7621 ctgttatgaa aaaatgctcg ccgccagtga gaaccacaaa gagatttcgc tgatcgaaca
     7681 tgcgcagttg gatcacgctt tccatctcgc catttgtcag gcttctcaca atcaggtgct
     7741 ggtgtttacg ctgcaatcat tgaccgatct gatgtttaat tcagtgtttg ccagcgtaaa
     7801 taatctctac catcgaccac agcaaaaaaa gcagatcgat cgccagcatg cgcggatcta
     7861 caacgcggtg ttgcagcggc tgccgcacgt cgctcagcgc gcagcgcgcg atcatgtacg
     7921 gaccgtgaaa aagaatctcc acgatatcga gctggaaggc caccatttga ttcgctcggc
     7981 ggtgccgctg gagatgaaca aggtgggaat gtgagcagag ttttacccta tccccaaggc
     8041 gaggggaccg cccctgcact taacaggatc tggtgttaaa tctgttcatc tataccctct
     8101 cccgaatgac cgcccctgcg ctaaacagga tctggtatta aatttgcttt tcatacaatc
     8161 ggtaacgctt gtacggctcc gccccaatgc gttccagcat gttattcatg cctgtattgg
     8221 tttcgaggat ccatgacatc tccagcgcat cgatcttccg gcgggcaaac ggatcgcgta
     8281 aggcttcaat caataacagc gcaatcaccg ggccgatgcg gctgaactga tactcgtcgc
     8341 gcacgcccat cagcggtact cgcgcagttc gcacaccgct gactttcaaa cgccacagca
     8401 attttgccca gccgaaggga aagagcgatc cgttcagatc ggcaatcgcc tcgttgatgt
     8461 taggcaagcc acaataaacg cgcagggtgc agaatcaatc tcagcgatgt agatcatatc
     8521 gtccggcacc agatatttaa gttggtcgcc catggtcgcg aattcatgtt cggtaaacgg
     8581 cacaaatccc cagttgtgct gccagccaga gttgaaaatc tcacgcagga tctgcatctc
     8641 ttcggcaaac cgctgacgat tgatgcagcg aatggtcacc tttttgcgca cctgatccat
     8701 cagttttttt agcgccggag agaaagtgag atcggttcgc tgcatccacc acgccagtaa
     8761 atcaatgcct ttgtgataac ccagttgttc aatatgcgcg gcataccacg gtttgccgtg
     8821 tggcatcatc gcacagggtg gtgtgtcaaa accttcaatc agtaatccgc tttcctgatt
     8881 gatattcagg ctgaaaggcc cgctgatctt acttgcacct tgtgacttca accacgcttc
     8941 cgctgcgcca aacaacgcgg caaaatcctg cggatcatca atggcgtcaa tcatgccgaa
     9001 atgaccggta tcttcaccgt aacgctcacg gtgcaaggta tctatttgcg cggtaatacg
     9061 cccaactatc tgccccgctt tttttgccac ccacgcctgc cagatgatat ggtccgtccc
     9121 cggatttttc gcagacaaat gctcgttgcg ttcaatgaat aaagggggta tccagtttgg
     9181 atcgtcggga taaagtgatg acggaaaagc gataaatgcc ttaaggtcat ttttattaat
     9241 tactttttca attttaatca tcagcagatc gcattccata tctctgtgga taacaaataa
     9301 ttaatggtga aaaacaggtt gcaacagcaa cgagcggcat cttaataatc aactcaatga
     9361 atacaaacga cgaataaata gtattaatta atgattatga taaggtcaga ggctacacag
     9421 aaacgcctca atgccttttg tcaatttaag gcgaccggac aaggcttctt cttaatatat
     9481 tcataaatta aatatcattc ataataaaaa caaaaaactt aaactcatta tttaaatcaa
     9541 tgttcatttt cacaccgatg aaaatactat ctcatttatc ttttatgcct atataatcca
     9601 ccgcattatt gtcattttat ttttgatatc caattttttc gtggtgtata tgtctaataa
     9661 aatctttacg cattccctac ctatgcgcta tgccgatttt ccaacgctgg ttgatgcttt
     9721 ggactacgcc gctctgagta gagccggaat gaatttttat gacagacgtt gccaacttga
     9781 agatcaactg gaatatcaga cattaaaaac gcgtgccgaa gctggtgcga agcggttgtt
     9841 atcgctgaac ctgaaaaaag gcgaacgcgt ggcgctgatt gccgaaacga gtagcgggtt
     9901 tgtagaggct ttttttgcct gtcagtatgc tggcttagtc gccgtcccgt tggcgattcc
     9961 aatgggcgtc ggtcagcggg attcctggag cgccaaactc cagggtttac tggcaagttg
    10021 ccagcccgca gccattatca ctggtgatga gtggttgcca ctggtcaatg ccgcgacgca
    10081 taacaacccc gaattacatg ttttaagcca cgcttggttt aaggcattac cggaagccga
    10141 tgttgcgctc cagcgtccag ttccgaacga tatcgcctac ctccagtaca cctccggcag
    10201 cacccgtttt ccccgtggcg tcattatcac ccatcgcgaa gtgatggcta atctacgtgc
    10261 tataagccac gatggcatta aattacgccc tagcgaccgc tgcgtctcct ggcagccttt
    10321 ctaccatgat atgggactgg tcggctttct cctgcccccc gtcgccacgc aactttcagt
    10381 agattatttg cgcactcagg attttgccat gcgtcctttg caatggctta aattgatcag
    10441 taaaaatcgc ggcaccgttt ccgttgcgcc gccgtttggc tatgaattgt gccaacgccg
    10501 cgtgaatgaa aaagatctcg ctgaactgga tctttcctgc tggcgcgtcg ctggtattgg
    10561 tgctgagccg atctccgcag aacaactcca tcaattcgct gaatgtttcc gtcaggttaa
    10621 ctttgacaat aaaactttca tgccgtgcta cggactggca gaaaatgcgc tggctgtcag
    10681 cttctctgat gaagcctccg gggttgtggt taacgaagtg gatcgcgaca tcctcgaata
    10741 tcagggtaaa gccgtcgcgc cgggtgcaga gacacgcgcc gtatcgactt tcgtcaactg
    10801 cggcaaagcg ttgccggaac atggtattga aatccgcaat gaagcaggta tgccggtcgc
    10861 ggaacgtgtg gtaggccata tttgcatctc cggtcccagt ctgatgagcg gttactttgg
    10921 cgaccaggtt tcgcaagacg agattgccgc gacgggctgg ttagacaccg gcgacctcgg
    10981 ttatctgctg gacggttatc tgtatgtcac cggacgcatt aaagatctga ttattattcg
    11041 tggccgtaat atctggccgc aggatattga atatatagcg gaacaggaac cggaaattca
    11101 ttctggcgat gcgattgctt ttgttaccgc ccaggaaaaa atcattttgc agatccagtg
    11161 tcggatcagc gacgaagaac gtcgcgggca gcttatccac gcgctggcgg cacggatcca
    11221 aagcgaattt ggcgtgaccg cggctatcga tctgttgccg ccccacagta ttccccgaac
    11281 gtcctccggc aagcctgccc gtgcggaagc gaaaaaacgt tatcagaagg cttatgctgc
    11341 cagtcttaat gtgcaggaat ccctggcata aatcaaactg tcgcggtgac gggcgctacc
    11401 gggtttatcg gtaaatatat tatcgataac ctgctcgccc gcggctttca cgttcgcgca
    11461 ttgacgcgta ctgcccgcgc tcacgtcaat gataatctca cctgggtgcg cggttcgctg
    11521 gaagatactc attcacttag cgagctggtt gccggagcca gcgtagtggt ccattgcgcc
    11581 gggcaagtgc gcgggcacaa agaagagatt ttcacccgct gtaacgttga cggcagcctg
    11641 cgcctgatgc aagcagcaaa agagagcggc ttttgccaac gttttctgtt tatctcttcg
    11701 ctggcggcgc gccatcccga gctctcctgg tatgcaaaat ccaaatacgt cgccgagcaa
    11761 cgactggcag caatggcgga cgaaattacg ctgggcgttt ttcgcccgac agccgtgtat
    11821 ggtcccggcg ataaagagtt aaaaccgctg tttgactgga tgctacgcgg cctgctgcca
    11881 cgacttggtg cgccagatac acaactctct tttcttcacg tcaccgattt tgcgcaagca
    11941 gtgggtcagt ggttaagcgc cgaaactgta cagacgcaaa cctatgaatt atgcgatggc
    12001 gtcgccggtg gctatgactg gcaacgcgta cgacagcttg ccgccaaagc ccgttgtggt
    12061 tccgtgcgaa tggttgacat tcctctgccg gtactcacag gccttgcgga tatcagtacc
    12121 gcgttgagtc gcctggcagg taaagaacct atgctgaccc gctcgaaaat tcgtgaatta
    12181 acccacgccg actggtcggc aagtaataac cgtatttctg aagatatcaa ttggtttccc
    12241 gggattagcc tggaacacgc attacgcaac gggctatttt gattgtcttg ttttaaaggt
    12301 gtgttaacgt ttctatggta aatcgtgaaa tagtaatgga ttacatcctt tcctgtttac
    12361 aggatttggt cgaaaacggt gtggaaatta agcccgacag cgatctcgtc aacgatcttg
    12421 gcctggagtc gatcaaagtg atggatctct taatgatgct ggaagacaga ttcgatattt
    12481 ccattcctat taatattctg ctggatgtca aaacccctgc gcaattaatg gaaactctac
    12541 ttccatggct ggagaataaa taatggggct atacgataaa tatgcgcgcc ttgctggcga
    12601 gcgtctgcaa tttagtgaca acgggttaac accgtttggc acctgtattg atgaagtgta
    12661 ttccgccacc gaaggccgga tcggcaataa aaaagtgatt ctggccggga ccaataatta
    12721 tctcggttta acctttaatc atgatgccat cagcgaaggt caggccgcgt tagcggccca
    12781 aggcaccggc accaccggtt ctcgtatggc aaacggcagt tatgcgccgc acctggcgtt
    12841 agagaaagag attgccgagt ttttcaatct ccctaccgct atcgtttttc aaccggttat
    12901 accgctaatt taggcgtcat cagcgcactt gccgaccata acgccgtggt gttactggat
    12961 gccgacagcc acgccagtat ttatgacgcc tgttctctcg gtggcgcgga aattattcgt
    13021 ttccgccata acgatgctaa agatctggaa cggcgcatgg ttcgcctcgg tgaacgggcg
    13081 aaagaggcga ttatcatcgt tgagggcatc tacagcatgc tcggtgacgt tgcgccgctg
    13141 gcggaaattg ttgatatcaa acgtcgtctc ggtggttatt tgattgtcga tgaagcgcat
    13201 tcgtttggcg tgttaggcgc aacggggcgc ggactggctg aagctgtcgg cgttgaagat
    13261 gatgtggata ttatcgttgg tactttcagt aaaagtctgg cctcgattgg cggttttgct
    13321 gtcggttcgg aagcgatgga agtgctgcgc tacggcagcc gcccgtatat ttttaccgcc
    13381 tctccttctc cgtcctgcat tgcgaccgtg cgctcgtcgt taagaactat tgccacacaa
    13441 ccagagctgc ggcaaaagct gatggataac gccaaccatc tgtatgacgg tctgcaaaaa
    13501 ctgggttatg agttaagttc acatattagc ccggtagtgc cagtaataat cggttcgaaa
    13561 gaggaaggtt tacgcatctg gcgcgagctt atctcgccgg gcgtgtatgt gaatcttatc
    13621 ctgccgcctg ccgcgcctgc aggcatcacg ctgctgcgct gtagcgtaaa tgctgcccac
    13681 agtcgtgagc aaatcgatgc catcattcag gcatttgcta ctctgaagca gtaatgtgag
    13741 cccgccgtgg tgtttgcgcc acggcgggtt taatgttgtc tgtcatacca atacaccagg
    13801 ctcaacgcca ccaacacaaa cgctaccggt aaaccgagcg cggtaacagg tgcgcttaac
    13861 gcaaatagca atcccagatt aaccatgatt tgatagctga tccaggtgag taagcccacg
    13921 atgacgccta cagcgagacg gctacccatc ccaggcgaac gcggggcact aaaggtaaac
    13981 ggcacagcca gcaaaatcat cgccagggtc aggatgggtt gccccagttt ttcccacagt
    14041 gcaaggcggt attcgctgct gggttgcccg gtttcctgca aataatggat gtaatgatta
    14101 agctgacgga cagaaaaagt attccccggc attgataact cttcaagatt catactggtg
    14161 aaggccgatt gccaggcaag attatctaat gtttccagcg tttctttgcc attcagccat
    14221 tttttatgat tcacaccatg caggacccac gttttgtcgt cttcaatggt ggcactgcgt
    14281 gcataaatgt aggattcaag cgagagatcg tcgcgataat gaaatatctc cacgcccacc
    14341 ggctggccct gctcgttcag cgatttcacc gtcacaaatt cattaccacg cctggcccac
    14401 agcatattgc cggtaatgtc atcgtcctcc cccaacgcgg tggcggtcga tttgatttgc
    14461 aacgcctgtt gctgcaatgg cgacgccacc cactcatcta tcgcgcctaa cgaaacagtc
    14521 cacaatatcc cggcaaccag cgccaccagt gcaatacgga agatagaaaa ccccgtgctg
    14581 cgaatggcgg taagctcact gtttttcgat aactgcccca gtccaacaat accacccaat
    14641 aacgcgataa acggcgaaag ttcgaccagt gtgcgcggta aggtcattag caccaccagc
    14701 accgcctgag tccagcgata accgcccggg ctgacaccat ccagttcgtt aatcaggttg
    14761 aaggtggtaa aaagcggcag caatagccct gcagcggcgg caaaaccgag aaagagatga
    14821 cggattaaat agcgactgaa aacattcatc gggaaaattt ctgcagcaaa gagaagtcac
    14881 gtgcgaccag tatcagcagg cccattagca tgagtccggg cactaaccac aaaccaggaa
    14941 tgaggggaat cgcgccgtta gccaccagtg tacggcagac gtcgccgcca taaaaaatgg
    15001 caataaacag caacgtcagt ggcaataacg ttgaaaatcg cccttgccgc ggttttaccc
    15061 gacttaatga aatggccagc agcgccatca acaatgcggt caggccacgg ctttgtcgcc
    15121 attgcagttc ggcatgatcg gcaggaaagg cggagcgcgc cagctccgtg accgatttcg
    15181 ctttgcgttt gacgttcggg ctttgatcca gcggattcag atgtaattgc agattacggt
    15241 agatctgttc gttgtcgtca cgcccctgat gatcgagaag ataggcagtg ccgttgtgca
    15301 acataacggt cggtttctcg ggtgatgggt caaccacatc aaccgaaccg gcgcggaaaa
    15361 tacgggttcg attggcagta gaagtataaa tcagcgcgtc agtcaggcga ttattatcct
    15421 gatcaaccgt ctgcgaaagg atcattcgtc cgttatcgtt agtgttaaat ttctttgccc
    15481 gcaactggcg aacatccagc tccgactgcg actgctgctc cagttgataa atctgcgcat
    15541 aagcccacgg acgaccatac atcgacagaa gggtgacaaa aatacttagc ggtatcgcca
    15601 gataaagaac ggctttgtac aagcgtcccg gactgccccc cgccgcagag atagcggtaa
    15661 tttccgagtc ggtatacatc tgccctagcg tcacgccaac tgacacatac agaccaacag
    15721 gtaacaacat ctccagtgca atcagtactt tgtagaaaac gatatccagc acaacatcaa
    15781 gagctaatgt cccatttgcc gcttcggtca gataacgctg tgcggagtag ctggcgaaaa
    15841 taaagatcag aaaaccgacg ataatcagca ccagacggcg cgtaccacgc atgatgtagt
    15901 gttcaaccag cttcatctca ctaacgccct caatcctttg agttttttat tcattgatca
    15961 gtttctgcgc caacgcaatg acatgatcta cctcttccgg cgtcaggctg ccgtcctttg
    16021 cccactgaat ttgcccggct ttattcagca ccagaatggt tgaactctgc tccggcaagc
    16081 gccaggcaac gcgtccctgt ccattgccgt caataacgaa ttgcgcccac ggatagcggc
    16141 gtttattttt ttcgattttg ccaacaacaa aatagccagt accaaagatg gcatcatcgg
    16201 tgttcacaat ggtggtgggc tgaaaacgat cctgcggaaa gtttgccgct tcaaccgctt
    16261 taattaacag tgagttcttt ttcttcgccg acttacgccc ggcgatatac tgaataatgc
    16321 ggacttttcc cgctaattgt gtgctgttcc acttttggta gtgaatttcg tcgtcgctat
    16381 tcagaagcag ctcgccgcct tcctggatat aaacaggggt aaccgttttg ccagtgacga
    16441 aattgtgtgc ctgcacaccc ggggctaaga gcatgataat caacgcaata atcaaacgag
    16501 atgacatcat tgctccggct atagcggaaa acgcgaaatc agcagctcta agcgctgaca
    16561 aatatcgcta tccagcgcca acggtgcata atacgactgg cggttttcca tatcatccgc
    16621 caccatgtcg tcgtgtttat ctcccatcag gccattaatg gcggcgagga aacgggaaat
    16681 ctgtaacgcc tctttatttt caggatcaag cgatcttaag gtgtcgtaat aagaaacctg
    16741 ctggctgatg atttttcgca catcatgaaa ttccctgccg gttagtcgcg gtttggcaat
    16801 aatctctttc agggtatgca tttgcttttg ccgataggca ataaaactgc ttacgctttc
    16861 taactggcta tggcgtaatg aatagttcac caacgaccag accggagagc ttaaataaat
    16921 ccgtaacagg ttgccgtaat acgacacaat atttttctta ccgttacgaa agccgtcctg
    16981 aaaacgcccc agaaaaacgg tcgttttgcc gaataaaaat ccggcctgat gcttctttaa
    17041 atacagccgt tgcgcaaaac gcaggtgctt gtagcgcgct cgcatgtatt taaattgttt
    17101 gcgctcatca gcagtcagct catcgccttt ggcttgtttg cgaactaaac gcagtaattc
    17161 ctgacgatca acgccattga gccagaattg caggcataac gcgtaattat catgaatgat
    17221 ttcggtggta caacttagtg caatagtcgg cggcaaacta acctgtgcat caacgacatc
    17281 atcttcttca atcgcaacaa atagacgctc gctcgcctca agcgggaaat attttctcat
    17341 catcaggatt tctctccggc tgcgttataa agcaaggaat ggcgctgaaa aaatgccttt
    17401 aaggttggaa ttaacgagag cgccacgtcc agacattgcc caccgaagta agcaaacgct
    17461 gctcccagca caccaaacag atgacagaac atgaacagca atacgatgta gccgattgag
    17521 gcgatggttt gcgccacgag aaaagcgcgc tgttttcccg ccatcaatag caatgactcc
    17581 tgcgggaagc ccagcatcga aatcacaatt gcgcccagca tcacctgaat caggtcatac
    17641 gcttcgagat acttaacgcc aaacaccagc gaaatgagcg gcttgccgac gatcaacacc
    17701 gcgagagcca ccagaatacc gattccgccc gccagtaaac cggacttcac acctaacaac
    17761 cacggtctgg tggtgcgcgg atctaaacgc atcacctctg ggtaaaaact tttacccagc
    17821 aaacccgctg gcgttccggc ggcgtcgaag aatgtcatgg cgattttaaa taacccggcg
    17881 gcagcgggtc ctaacacgat ccccaccaac actgtgctgc acgagttacg cgccgaccag
    17941 atggagtggg caatgttggt tgaccagaca aaactccacg cgcctttaat gtgtcgggca
    18001 gactcaaaca gattcaattt gaaggcgtta tggatatttc tacggcgtaa ttcgcgcgcg
    18061 gcaaaccacc agtacatggt gccgccaacc agattcgaca cgtaccaggc aataacaaaa
    18121 cccgcaaaac caaagtcaaa ataccaggct gcgacgctcc ccgctgcgcg cagaaaaggt
    18181 ttcgtcgcct gctgtacagc aatcaaatca aagcgatcta ccgcacgcag aatgccggtc
    18241 ggcgtggagg aagccattga aggaatgagc gtgcaataca gcgccgccag ccaaaagctt
    18301 tggtcatcca gacctaatga atgggaaagg aatggcagta aggcaatgcc gccgacaatc
    18361 gccaccgcgc cgctgacgat atccagcgag aaggaaaatg agacgacatt gcgaaattgc
    18421 tgcggattat tgttggtcaa ggctggcgtt ccgtactgaa ccaccagctg ccatgtctga
    18481 aacttaataa aatcgctgat cgacttggcg tacgattgca caatcaccag tacaccaaac
    18541 atggcgggcg tcatcccttt accggcacac gagagcgcca acagccccag caaggcgctc
    18601 tcgacattac tggagcctaa ccaggcgcta ttgcgaataa tggtgcgaaa cgcgccatct
    18661 gcaaaccaat gtttgatgtt aaaacccgcc aattcagcct gaccttatcg ttgatagtaa
    18721 aaaatatccc gccagcctta agttaaactt cggcggtcag aaacgatggc aaccagagaa
    18781 accgccttct gtacctgttc cagcacttcg ctgtagggcg ctctggaatc aatctcaaga
    18841 atttttgtgc cgttatagcc aatcgtcgac atgacgccga ttttgtcctg cagctcggca
    18901 tactcatggt caggcttgcg ggagatggca gtatcaatat caatccccaa acgaatgatt
    18961 aattcaggac ggtattgcgc cattttttgg tataaactcc gttcgcgctg cgccagaaac
    19021 atgctgattt tcccggtcgc acgttcgacg ccgatccccg gtccgtcata ataaaagccc
    19081 gaaatttcag cctgcgggaa gcgatcgctg accaccagaa cgccactttg cgccagtcgc
    19141 tgaaccttgc gtagattcgc cattcttcgc agcgagaagc agtacataat caccgccgcc
    19201 catagcgccg gagatttggt tttcatgctt tgggttttcg aggatttggc cgccaatcgc
    19261 cgttccagcc agacgccaac caacggcaat cgtttgattt tgtcgccgtc ttcgccggag
    19321 agcagcccca gatagcggcg ctcggtttgc cagtgttgtt gcagcgattt caccaggtcg
    19381 gtggtcagcg tggatttacc ggtgccatca cacccgacca ccgcaatcag ccccggaatg
    19441 tagttgggct gcggcgcggt tgtactatta acagtttgtg tttgtagtgc atccattcgt
    19501 taaatcatcc cctatccatt ttggtgatca ggaaagcgcg gcgtgaattg cgcgtagtga
    19561 cgcttgcata atttcatcgg ctggttgccg accatccagt tcaaggatct tggcgccatt
    19621 gaatgtcagt tgcggcgtaa cggcgatttt ttcctgtaac gctgccagtt ggtggtcagg
    19681 tttacgcgca aacgcggttt gttcatcaat gccaagacga atcaacaata cgggtaaata
    19741 agacgccatc cattggtaca gcttcaactc gcgctgcctt aacattttta tccaaccgtt
    19801 accgcccgtg gtttttgcca attgcgggcc atcaaagcga aaacccggca cttcaacctg
    19861 cgggtagcgg tcggtgatga gcagatagcc ttgctggctt ttacacaaca ttttgcgaaa
    19921 cttgtacgcc cgccagcagg aaagcagata gataaccagc gcagtaatat tgccaggcgg
    19981 agttaagggc ttttcgtgta catgtgccgc tttacttcgc agataacacc cacaaggtgc
    20041 gccaataaca gggagctgtg aaatccattc gccaattcgc ccggacgatt gcccgagata
    20101 aatgtgttct gttggcattc ttgctgccag ttcatttacc aggcttgccg tgagggtcga
    20161 tttacctgaa ccatcacatc ctacaatggc aataacacgc actggcgtgg aattaattat
    20221 tgacatatta cgttgattca cgaaaaaccc ggcagtaaat aatgtattga atattacgtg
    20281 gtccgtgctc agaatatccg ttcaacctta ttgtgacaat ggctaattgt gacattgacc
    20341 tgaattacgc tttcataaaa acatattaac caaataaata tttttaatgg atatttaaat
    20401 taaaggatat attcatgcag tcaataacac ctccattaat tgccgttatt ggtagcgatg
    20461 gttcaggcaa gtcaacggtg tgtgaacatc ttattaccgt tgtcgaaaaa tatggtgctg
    20521 ccgaaagagt tcatttagga aaacaggccg gaaatgtcgg tcgtgcagtg acaaaattac
    20581 ctttgatggg aaaatcctta cataaaacaa ttgaacgaaa tcaggtgaaa acagcaaaaa
    20641 aattgcctgg accagttccg gcgctggtaa ttacagcgtt tgtcgcccgt cgcttactgc
    20701 gctttcgtca tatgcttgcc tgtcgtcgtc gcgggttaat tgttctaacc gaccgttatc
    20761 ctcaggacca aattcctggc gcttacgatg gtacggtgtt cccacctaac gttgaaggtg
    20821 gtcgttttgt ctcatggctg gcaagccagg aacgtaaagc gtttcactgg atggcgagcc
    20881 ataagcctga tctggtcatc aaactcaatg ttgaccttga agttgcctgt gcacgtaaac
    20941 ccgaccataa acgggaatcg ctggcgagga agattgccat aacgccacag ttaacctttg
    21001 gtggtgcaca actggttgat atcgatgcca atcagccact ggaacaggtg ttggttgatg
    21061 cagaaaaagc gattacggat tttatgaccg cgcgtggtta tcactagtca aaaatggaaa
    21121 tgcccgatcg ccaggaccgg gcattttcag gaaggttaaa tcaactgcaa tgctatccag
    21181 tacagcccac cagaaagaaa aattgccgcc ggtaaagtaa acacccacgc catcaggatg
    21241 ctggttaccg ttttacgctg taacccaccg ccgtccacca ccatcgtccc tgctaccgca
    21301 gacgagagga cgtgtgttgt ggagacaggc atcccaatat aactggcaag gccgatagac
    21361 actgccgccg tcatttgtgc cgccatgcct tgcgcatacg tcatgccgcg cttaccaatc
    21421 ttctcaccga tggtcatcgc tacacgacgc cagccaatca tggtgccaat gccgagcgcc
    21481 agtgctaccg ccatgatgat ccacaccgga gcgtactcaa tggtgcttaa catatcgctg
    21541 cgcagttttt tcagcaggtt ctggtcttct ttactgacgc ctggcagttt cgccagcttc
    21601 gcggaggtat cggagatgca cagcataatg cggcgcagct ggctgcgctg actcacgctt
    21661 aacggctcgt aactttccat attgcctggc agcatcgttt taacgcgcgc aatcgcatca
    21721 aaggtatttg ccggatgaca gtgaaactct gttacttgcg tgccatcagt cgatgctgca
    21781 ggcaatggag gttccatcgc aatcaacttc tgcggcagtt caggatgctg ttgcaggtag
    21841 tgttcgaagt tggtaacggc atcgcgggta cgggtaattt catagccgga cgcattcata
    21901 ttgacgacga agccagcagg ggcaatcccc accagtacca gcattaccag gccgatccct
    21961 ttctgcccgt cgttcgcgcc gtgcgaaaac gccacgcccg cagcggaaac aatcagcgca
    22021 atacgcgtcc agaatggcgg tttacgtttg ccctttttct ttttgcgatc ttccggaatg
    22081 cggtgaatac ggtcacgctt tttcgtccca ctccagaaac ggcgcagcag gaaaatcaga
    22141 ccacccgcaa tgaccaggcc gacgataggg gaaacaatca gcgaggagaa aattttggtt
    22201 acttcacgca ggtttaacgc atccatcacc gatgagccgg ttaacagcgc gttggttaaa
    22261 ccgatgccga taatcgcacc aatcaaggtg tgcgaactgg aggccggtaa accaaagaac
    22321 cacgttccca ggttccagat aatcgccgcc agcagcatgg aaaagaccat cgccaaaccg
    22381 tgagttgacc ccatattcag caacaaatcg attggtaaca tatggacaat ggcataggca
    22441 acgctaagtc cgcccaataa cacgccaaaa aagttaaaaa atgccgccat caccacagca
    22501 agttgtggct gcatggcacg agtataaata acggttgcca ccgcattcgc cgtgtcatga
    22561 aaaccattga ttgcttcgta gaacaacaca aatgccagag caagtaataa caaaagcccc
    22621 gtatatatat caaggccaac aaataaattt agcatatatt agattacgcc attttgaatt
    22681 tacgaacgga cgcattatca gtgactttaa cggcatgggc aaagtgaaat atcatttttt
    22741 aacgataaaa agagtgttat ttcttgtgta tcagacaata agcattcatt caaaaccttt
    22801 agaaataatc aaaacacagg tattccactg gttaatatat tcacatatga aatgaataaa
    22861 tattggaata tataaatatt gaatattttg attaatcccc gtactaatta ttcttcataa
    22921 tcagtacggg gttgctacaa catgtattac tttttcacca caattaacgg ttcaatatca
    22981 ctctcttttt tgatgaccag tgattcatca ccgcgcaaac acgtcccacc gtagttgccg
    23041 ccaacggtga aggtacatac ctgaatgtat ttaccgtcca ctttcggcaa acaccacagt
    23101 tgctgataga tgtttttctg ctcggcaaat ttaccgctgg ttttgtccag cacctcttca
    23161 tgatggctga ccaggtcgat attgctacca cagcgaccgg cgatcggttt cactgcgtaa
    23221 cctgttttca ccaattcatc attaacagtg aaatcggtat ccagcaggta acgatggtgc
    23281 gggaacagcg accagaggat cggcagaatc gctttgttgc cggggatcac cgtccacagc
    23341 ggctcaaaga ccagcacttc cgggcgcagc aatacgtcga taagacgcac ttcgttttgc
    23401 ggatgaccgg tacggattgg caccgcagca aactcacggt cgctaacttc acgaatctga
    23461 tcaaacgcgg tttcccacgc ccaggttttc cacacgcagt taaccagtcg cccttcccca
    23521 tcaatcagtt gcccggcagc atcccagccc agttcatcca gcccgcgcaa gatacgcgtt
    23581 tcaaagcccg cctggtgcag cgcctgctcc ataaactgcg cgtgatagtt ttcctcgata
    23641 tctttgtcct gcatgatatg gacaaacgga cgtgcacgac tgtgtttcca ggcaccagcc
    23701 agttcgttaa tcagcccttc cgccggattg aagccgttgc ctttatagcc ctgctccgcc
    23761 caacgttcga ggatcaagcc cgcttcggta tgacaggagg cggagtcggc gttgtattcg
    23821 taaaccttca ggccacgctc atccatgcag aaatccatac gaccagtgat catatggtga
    23881 cggcgacgct gccaggagag acgcaaacgt ggccagagga ttttcggaat gtcgaacagc
    23941 gccagcaggt tgtcatcttt cagcaccttg tcggttgcgt gaagatacat cagatgcagc
    24001 tcgttggtgg ctttaattag ctcctgctcg gcactctcgg taatggtgta gtaatgatac
    24061 ggatcctgat tgatcacctg accgttggcc tgcacatagg cgttttgtag cggatctttt
    24121 tcatccagcc atttaccgtc aaactggcct ttgttttcca gacgcgctcc actgattttc
    24181 agcagctcgc ctgcaatttc cggctgcggt aagctgtatt cagtatcttc cgtctggatc
    24241 atccagccca gaatggtggt gtcatcaaaa gtgtctttaa gggtatagca gccgttttcg
    24301 accaccatct ccagctcgcg cgtccactgt tgcccttgcg gcaacgggga atgaatcacg
    24361 ttctgttccg caatacggac tttgttgcca tgcaattggg taatgatggc gacatggcca
    24421 gtgtctttaa attcaccgcc tttatcccag ataagaagcg cacccgcgac cggcgcacgc
    24481 ggcgagccgt taggaaatgc ctgcaatggc aggatgttgt cattaaccac ttctcgcagg
    24541 aagcgcagcg agaaaatctc ccacgccata cccacgtcag taaagaccac accgtaattc
    24601 agaaagagaa aacggcgagc aaattcaacg cattgccact tgtggcccat atattcgtcg
    24661 tcgatatagc tacggaatac ggcgtcatct tcgtattcct gcggatcgag agaactgtaa
    24721 tctgaagagt agattgctac cccacctggg gcgtagccca ataatgtccc gaacggggca
    24781 tcctggctgg tcgttccttt gctcatcact ttacctttaa caatacaacc taagcagttg
    24841 gcgtaattgt tgtgctctga ttacctgctc agcgacatta accggacaga ggtcagacta
    24901 atcatacacc tcatcgcagc ggctggcgca aagggttaaa aaattcacat tctgtacagc
    24961 aagtgacctg ctacactgct tcaacactac cactcagaag gcaactcact atgacagaca
    25021 atacttatca gcccgcgaaa gtctggacgt gggataaatc cgctggcggc gcgttcgcca
    25081 atatcaatcg cccgttttct ggtccgacgc atgaaaaaac gctgcccgtt ggcaaacatc
    25141 cattgcaact ttattcgctg ggaacgccga acggtcagaa agtaacgatt atgcttgagg
    25201 agctactggc gctgggcgtt actggtgcag agtacgacgc ctggctgatt cgtattggcg
    25261 atggcgatca attctccagc ggctttgtcg aagtgaaccc aaactcgaag atcccggcgc
    25321 tgcgcgatca tacgcataat ccgccgatcc gcgtgtttga atctggttcg atcctgcttt
    25381 atctggcgga gaaatttggc tacttcctgc cgcaggatct ggcaaaacgg actgaaacgt
    25441 tgaactggct gttctggtta cagggtgccg cgccgttcct cggcggtggt tttggtcact
    25501 tttaccatta cgcgccggtg aaaattgagt acgccatcaa ccgctttacc atggaagcca
    25561 agcgcctgct cgacgtgctg gataagcaac tggcgcagca taagtttgtt gcgggcgatg
    25621 agtacaccat tgcggatatg gcgatttggc cgtggtttgg caacgtggtg ctcggcggcg
    25681 tgtatgatgc cgcagagttt ctggatgcgg gaagttataa acatgtgcaa cgctgggcga
    25741 aagaagtggg cgaacgtccg gcagtgaaac gtgggcgtat tgttaaccgc accaacgggc
    25801 cgctgaacga gcagttgcat gagcgccatg acgccagtga ttttgagaca aatacagaag
    25861 ataagcgtca ggggtaaggg ttggtgttcg tcgcagcaag ccatccaggc cggataaggc
    25921 gttcacgccg catccggcaa tcgtgcacaa tgcctgatgc gacgctgccg cgtcttatca
    25981 ggcctacaaa agctaatcat cacgcgctgg taatgtcgta atccatttgg cgcagtgcgt
    26041 ctaatgtggc tttggcttca tcttcgtcga tgatgctcat ggcaaatccg acgtgtacca
    26101 gcacccactg gcccagcaga tcggcagggt taccttcgca aatcagggcg atattcacgt
    26161 cgcgcttgat accacatact tcaacctgcg caagctggtg aatatcttca ccgacagcca
    26221 gcacctggcc tggaacgcca atacacattg ttaactccgg ttatttcgat tgccggacaa
    26281 acgcgaaaaa gaacctttag cgggtgttcc ctctcaccct aaccctctcc ccggaggggc
    26341 gaggggaccg tccgtgcgct ttttaggcgt tgtcctcagt cttattcaac ttcaatactt
    26401 ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac
    26461 tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat cccagcacca ggcctgggcg
    26521 ggtttataga cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca
    26581 aaactaaaac ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg
    26641 gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc
    26701 tggcaaagcg acaactcatg cattttcgct cctcctgcgg ccaaacagca gggcgcgacg
    26761 ccctgcgtgt ggtacatccg gattggtgac tggcaacgaa aggatcattc gtgcgcagtc
    26821 atccgtcagg cgttggcctt cttcaatcga catgctgtgc gaaagcggcg acatcagcga
    26881 acaggagagg tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tggtgccata
    26941 cggcagttgc agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc
    27001 ggggaagata actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc
    27061 gaacagcgta aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg
    27121 catcgaacgc cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg
    27181 gaaacctgct atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt
    27241 cgcgcagcgc agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg
    27301 ttaagccgat gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg
    27361 gaaactcacc ggtgaagcgc agggccgaca atacgtcggc caggccaagc tgatgcggag
    27421 agattttgtt ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg
    27481 gagtgttctt tttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt
    27541 cgccaagcag ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa
    27601 tgtatcgttg ctctaacgct tcgacaatcc gcacgccgat ggcttcatcg gtcagcaaaa
    27661 tattgccgac ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt
    27721 gccgtcggca tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt
    27781 acgcaccact tccagcggtt tattcggatc ggcaaccggt gtacccacca gcgactgctc
    27841 gtaaggaccg acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac
    27901 cgcctggtag ttgctgatga taccgtcttt aataaccatc cagtgagaga gcataccgcg
    27961 cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggcttcac
    28021 aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag
    28081 gatatcctgc aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg
    28141 cagctgtgcc acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt
    28201 cagtttgttc tgggtagatt cgcgacctgc cgccagtttc accagcatat tagccagcgg
    28261 acccacttct accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc
    28321 gtcagaccaa ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc
    28381 gtctttatac caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga
    28441 atgagaagtg atcggacgat acgaggacag atccgcattc tcaatgtagc ctcccgggaa
    28501 caggaagctg ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc
    28561 acctttaccg cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt
    28621 ataaacctgc tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg
    28681 ctcaaggttc aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc
    28741 caggttctga atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg
    28801 ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag
    28861 tttcatcgcc gggtgacccc agtagccatt ggcgaaaata cccaactgac cgctggcaac
    28921 cagatctttg atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt
    28981 cgaaacgcct ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat
    29041 gtccacccag tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg
    29101 cgcagccaga atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc
    29161 actttctgcc gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg
    29221 ttgcacaatc atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg
    29281 ccacatggta ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat
    29341 gcgtaaatgc ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg
    29401 ctcgcctcat gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga
    29461 atgagtacga tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct
    29521 tcccaggtcg ggaagtaggc gtaaccgccg cccgggttga atgccaccaa cgaataggtc
    29581 agacgccagg ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa
    29641 tcattacgca gcttcgccac acgcagaacg accagcggga agagcatcag caggacttca
    29701 atccagaaca tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag
    29761 ataagctcgc caaagcgcag cccgatgaaa atcgccagca acacactgat ggtgttggtc
    29821 agcttgacga acaggctctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagt
    29881 gaaccttcaa agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc
    29941 ggcaacattt catagctctg ccagagcgga tgcaccttgt agcccgccga gatcatcagc
    30001 gaccccattg aagactggtg catggtcggc agcagcgcac cgagcgcgat gatgaagaac
    30061 atcaccttgt tcagacgttg tagcgacacc ttccagccca gacgttcaaa cagtgccgga
    30121 gcaaactcca gagccatcac gccgatgtag atggtcatac agaccgccgt ctcgaacagt
    30181 accgagttca cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc
    30241 acgtcgatag tgatcgacaa gccacccagt gagtaaccaa acagactcgc caacagcgcc
    30301 ggacgcacca gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc
    30361 cagccgccac aggcaaagcc ggtgccaatc agcaggtcaa acgcgatcca cacgccccac
    30421 gggaagccgc cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc
    30481 aggagcatac agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg
    30541 ccgcccagcg gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt
    30601 ggtgttgcga cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc
    30661 tttatacagg gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt
    30721 cggcaggtcg agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc
    30781 ctctttctcg ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc
    30841 agatttcagc gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt
    30901 tttcgcctcc gccatcagct cttcacgcgt accgaaaatc accgcgcccg ccgggcacac
    30961 ttctacgcag ccaggcagac cgcctttatc gagacgttcc acacctttct ggttgcacag
    31021 ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtacgg
    31081 acaggcgacc atgcagtaac ggcaaccggt gcacacgtct ttgtcgtaat ggacaatgcc
    31141 ggttttcgga tcttttttca gtgcagagac cgggcacaca gagacacagt tcggatcgac
    31201 gcagtgcata cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac
    31261 ccctgtgccg ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt
    31321 gttcgaccag gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt
    31381 ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc
    31441 cggaattggc gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag
    31501 caatgccccg caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt
    31561 gagtcagcgt tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca
    31621 ccggcaacca gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg
    31681 ccctctttag cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca
    31741 agctgatgga tgcctttatg gaagccgata cctttttcgt tacagccata gcacgggtga
    31801 ccaatcgcca ccggccacac accgccaaca tcgcagaatt gcagcgttga gcagttgccg
    31861 taagtttctg gccctttaca gccgaggtgg tacaggcacc agccttcgcg gtggccttca
    31921 tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg
    31981 tgaatcagac ggccataggc gaaggtcgga cggtttttgt catccagttt cggcggtttg
    32041 ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc
    32101 ggaatattga taacggtttt gcctggcaga acttcttgca agctgactgc gccagttggg
    32161 ttaactccag ctgcggcaac accgccccac gcggagcagg aaccaatagc gataatggct
    32221 gctgagcctt ccgccgcttt gcggatgtga tccacaatcg gctcaccggc aaccatgcaa
    32281 taaataccgt tatcttttaa tgggatggaa ccatccacca ctaacacata ctgccctttg
    32341 tacttctcga gagcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc
    32401 acttcgtgat attccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt
    32461 gcacgaagca gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc
    32521 ggacgctgcg ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc
    32581 atggtggcgg ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa
    32641 tggatgaggg tgttatctcc agtcatttat agttattccg ttgcgaggac ctggcatata
    32701 ttttgcctca atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta
    32761 tggttatcga tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg
    32821 taattaatta aagcaagata atacgtatgt ttgatcaatt ttcgaggcaa taaccggcca
    32881 taaaaaagcg caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt
    32941 tatccagtcc atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc
    33001 cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac
    33061 agaattttgt cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc
    33121 ctttaccaaa atggttattc atcgttcacc tcattcgtta tatatcgcca tatatagcga
    33181 tattttaggc aaagaacgac ttttgcgcac gctatttttt catacttgtt taagacgtaa
    33241 tcccgtaaga gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc
    33301 caggcaagat cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga
    33361 ttaacccccg gatagatata agcctcataa accttattat tgtctttcaa cgccgcctcg
    33421 taaggaggcc agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc
    33481 gcttcaatct tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag
    33541 gccagttccg gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg
    33601 gtaataccca ctttacctgt cgcttgcgga tagcgttgca taaactcaat tgcggcaaag
    33661 aaatcattca tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca
    33721 tttcccggat aacctccaac ggaacttaat ccgtcaggtg ccagggcgat ataccccgcc
    33781 ttcgccactc gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc
    33841 acccccacgg ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc
    33901 tcgccgtgac catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta
    33961 aactctacct gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc
    34021 gtcatcccgc ccaccgcata cttcgccgca aggttgagga actcacgttt cgaaattttc
    34081 ccgtgaacgt aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa
    34141 cgcggcatcg ttgcattcct caactggtgt ttttttaagc aaagcataag cgcgtgtttt
    34201 tgcccagttt ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa
    34261 actagaaaca ttgtttcgaa attgaacgga ggaaaggaga ggtcatggtc tggttagcga
    34321 atcccgaacg ttacgggcag atgcagtacc gctattgcgg aaaaagtggt ttacgcctgc
    34381 ccgcattatc gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc
    34441 gtgcaatcct gcgtaaagcg tttgatttag gcattacgca ctttgattta gccaacaatt
    34501 acgggccgcc tccaggaagc gcagaagaga actttggtcg cctgctgcgc gaggattttg
    34561 ctgcttatcg cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc
    34621 cgtacggctc tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc
    34681 gtatggggct tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga
    34741 tggaagaaac cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg
    34801 ggatctcctc ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga
    34861 aaattccgct tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa
    34921 gcggcctgct ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg
    34981 ctcagggatt gctgaccgga aaatatctca acggcattcc gcaagattca cggatgcatc
    35041 gtgaagggaa taaagttcgt ggtctgacgc cgaaaatgct taccgaagcc aacctcaaca
    35101 gcctgcgctt attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt
    35161 taagctggtt gctgaaagat gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg
    35221 agcaactaga ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg
    35281 cgcagattga tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata
    35341 aatgacctgt taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga
    35401 gtcagtcggt caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg
    35461 acgtaccaca tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtaag
    35521 tgaatggaag aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc
    35581 attttcccca gccacttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa
    35641 ttctcataac cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc
    35701 agcgacagca acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg
    35761 tgaataatct cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag
    35821 tacacggggg caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata
    35881 cattcctgtc gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca
    35941 acagcaaatc aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc
    36001 ctggttccct tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg
    36061 agaaaaacga tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc
    36121 aaacagaaac aaccgacgcc aggcatccag gcgaagatga caccgatacc ggattgcggc
    36181 gagaaaacct atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc
    36241 gattccggaa taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg
    36301 atcagttatc ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa
    36361 tgcggacgca aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg
    36421 ctggttcacg aagcgcacaa ggcgttaggc gggctggata ttatggcgct ggtcgccggg
    36481 aaacaggttg ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt
    36541 gccattaacg ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa
    36601 ggtgcaagtg tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg
    36661 gactatgcgg ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc
    36721 gcggagaaag gtattcgggt gaatattgtc gcgccaggcc ctatctggac ggcactgcaa
    36781 atttccggcg gacaaacgca ggataagatc ccgcagtttg gtcagaaaac gccgatgaag
    36841 cgtgcggggc aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg
    36901 agctacgtca ccgcagaagt gcacggcgtg tgcggcggcg agcatttagg ttaaaaaaat
    36961 gcccggttgt gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa
    37021 aacgccttat ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc
    37081 ctgataagcg tagcgcatca ggcattgagc acctaatgcc tgatgcgtcg tgaacgcctt
    37141 atccggccta caaacgcacg aagattcaac atgttgcaat agattttgta ggcctgatga
    37201 gcgtagcgca tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac
    37261 ctatcttcag gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat
    37321 cgacggtttt gtccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat
    37381 tcaacgccgt aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt
    37441 ctgccttcac cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag
    37501 caggcaagtt caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca
    37561 gcagaaccaa catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat
    37621 cgtcgaggtt ttcgttaaga tgcattgcca tcggacatta tcctgcgcgt aatttttgtg
    37681 cgacacgaac cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca
    37741 acaatacctg cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa
    37801 atacgttata gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca
    37861 gagcttctgc gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac
    37921 caataaagct gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg
    37981 cgccgatggt tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc
    38041 gacgctccag acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg
    38101 acagttccag ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa
    38161 aatcagcggc gatatcgttg gcctggttta aggaacgtgc ttcagccagc agttgctgct
    38221 cgcgcttaag gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc
    38281 aggtgactac agaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt
    38341 actgatacat accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc
    38401 tccaggacgc aagtcacaaa atctgcgcat aataatatca aaacgacgtc gaattgatag
    38461 tcgttctcat tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc
    38521 agtaaaaaag tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag
    38581 gatgcgcccg cgaatattca tgctagttta gacatccaga cgtataaaaa taggaatccc
    38641 gacatggcgg acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac
    38701 actcttggcg cggtaaatag cgtgattcag cgcgcttctt cgctggtatt tgagagtatg
    38761 gaagccaaaa agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc
    38821 ggaacgttaa cccatttttc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc
    38881 tgcgcgctat ttccctgcgg ggcggcagcg gttgctaatt cgattcttgc ttttgtcgaa
    38941 cagggcgatc atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc
    39001 aaaatcctca gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat
    39061 atcgttaagc atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc gggttccatc
    39121 actatggaag tccacgacgt tccggcgatt gttgccgccg tgcgcagtgt ggtgccggat
    39181 gccatcatta tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt
    39241 ggcatcgatg tttctattca ggccgccacc aaatatctgg ttgggcattc agatgcgatg
    39301 attggcactg ccgtgtgcaa tgcccgttgc tgggagcagc tacgggaaaa tgcctatctg
    39361 atgggccaga tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta
    39421 ggtgtgcgtt tgcgtcaaca tcatgaaagc agtctgaaag tggccgaatg gctggcagaa
    39481 catccgcaag ttgcgcgagt taaccaccct gccctgcctg gcagtaaagg acacgaattc
    39541 tggaaacgag actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc
    39601 aatgatgaag agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg
    39661 tggggcgggt atgaatcgtt gatcctggca aatcaaccag aacatatcgc cgccattcgc
    39721 ccacaaggcg agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat
    39781 gtcgacgatc tgattgccga tctggacgcc ggttttgcac ggattgtata acattgccac
    39841 ttttggacaa ttttgcagac attttattgt gaaaagtctt aaattgttgc gtccgggatc
    39901 aaggcgtccc ggacgattca ggagtacaat aggcagataa aggcttaaac gctgttccac
    39961 aggaaagtcc atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc
    40021 cgcgctggcg gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt
    40081 tttagaaaac ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc
    40141 aggcgcattg attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc
    40201 cgcagcaagt ctgggctgct ggctaagtta tattcagggg agctggttag ggaataccaa
    40261 aacggtgaaa ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat
    40321 gtttgaccgc cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac
    40381 gctgctgcca accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa
    40441 ctggttaagt ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat
    40501 gattccgttc gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat
    40561 tgccttgtta accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg
    40621 taacgcctga cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct
    40681 gcatcgcccg cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact
    40741 cacggctaaa ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca
    40801 tgccgtcatg gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca
    40861 acggcgaggt gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg
    40921 cttctgccgc cagttgctcg acgctcaggt tttcggtgta tttattctca attcgtttca
    40981 gcacgcggct aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc
    41041 cgcaaggtcc ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac
    41101 gcgcatccag tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt
    41161 ctgataacgt ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt
    41221 cgccaatgtc catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg
    41281 ccagcggcac ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca
    41341 gcaggtattc attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc
    41401 cggaaaagag aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct
    41461 cgccatacaa caaacgcaca tcgggcaaca gttctgacaa actattttct ttatttttca
    41521 gtttattaac tttatccgcc agcaagcgac aaatctcttc acgtttcata tcgcgtaatt
    41581 tcttaggaat aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct
    41641 ggagaaatag gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac
    41701 aatgaaaaac atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag
    41761 taatgaacaa ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg
    41821 ctggtttacg cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca
    41881 gcgtgaaaaa aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc
    41941 tggaatttgg cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac
    42001 tggttcgcga acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg
    42061 gcaccaaatt tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc
    42121 tgcaaacggg cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc
    42181 cagcaaccgg ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca
    42241 agcaggcgtt ccattctgca catgttcagc cggtatttgc cgtgctcgat ccggtttata
    42301 cctacaccct gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg
    42361 tggaacagta tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca
    42421 ttttgctgac gctaatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg
    42481 tgcgcgccaa cgtcatgtgg gcggcgactc aggcgctgaa cggtttgatt ggcactggcg
    42541 taccgcagga ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg
    42601 atcacgcgca aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca
    42661 agcgcgctaa gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgacg
    42721 atgagcgtat tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc
    42781 cgacccacct ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac
    42841 tggaagagca cggcatgacc caactgggcg aaaatcatga cattacgttg gatgtcagcc
    42901 gccgtatata cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat
    42961 ttcgtccaga cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa
    43021 ggaggaacgt atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca
    43081 gctgggactg ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc
    43141 gttagaagtg ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt
    43201 cggcaaagcc ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct
    43261 gtggaacgac gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca
    43321 gcttgattat atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt
    43381 cgaagcatgg aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt
    43441 gtgcaacttc cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt
    43501 gataaaccag atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc
    43561 gacacacaaa atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt
    43621 cgatcagaaa gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt
    43681 tatccgctgg catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg
    43741 tattgccgaa aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcaaaat
    43801 tgcaaaactc gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaacg
    43861 tgcaaattct cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc
    43921 ccctcgcgtc ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc
    43981 atcatgcaga gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac
    44041 ggagaggtga aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta
    44101 ccgtacacgc gcagcctcct tcacagaccg tagaacaaac agtccggcag atttatcaga
    44161 actataaatc agatgccagt acgccttatt tcggtgaaac cggagagcgg gcgataactt
    44221 cagcgcgtat tcaacaggcg cttaccctga acgacaatct tacactaccg ggcaatattg
    44281 gctggctgga ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgttagaaa
    44341 gcgtagcgat aacccaaact gacgtcgatc atgccgatgc cgttgtgcgc tttcgtatct
    44401 ttaaagatga taaagaaaag accacacaga cactgaaaat ggtggcggaa aatggtcgtt
    44461 gggtcattga tgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga
    44521 atgaaaaaac gctggccgct ttagcttcat tgcagaaaga acagccagaa tcctttgttg
    44581 ctgaactctt tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact
    44641 cttaccgcca ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg
    44701 atgaagatat gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag
    44761 agtcgctatt ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc
    44821 gcattcatgt tcgttttacg ctgaccaatg gcaacaatga agagcaagag ctggttttac
    44881 agcggcgcga aggtaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac
    44941 ttaagcagat tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca
    45001 attagccgga acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc
    45061 tgttgttctg gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcaact
    45121 ggcgagcaat gtacaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc
    45181 actacgccca caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga
    45241 tgcacaggac tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa
    45301 tccccggcag catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc
    45361 gtttcttcgc accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag
    45421 caggcgtctg acgctgagtc gccatcggcg tatgtttagt caacgccgga cgggtattgc
    45481 ggttctggcg acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc
    45541 gacggctgcc aatcagatgc tttttgccca tcgcttccag cgcctgacgg attaacggcc
    45601 agtttgccgg atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct
    45661 tcggtacgaa gacgccttcg ctcttataac caatcttcgc cagcggtttt ttcccggtgt
    45721 aatacatggt ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac
    45781 ggaaacgatg ctttttcagc cacagcgcca aattcaccat atcttcatca cgcgtaccgg
    45841 ggtgcgcaga gatgaaatac gggatcagat actgctcttt acctgcctgt ttcgagtagg
    45901 tatcgaacag ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgaca
    45961 acggcccttc ttcggtatgt tccggagcaa tcttcagata accgccgacg tgatgggtcg
    46021 ccaactcttt gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga
    46081 taaggatttt tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt
    46141 cgtggttagt gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag
    46201 tttgttcagc gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga
    46261 gatcggaaat cacgcccgta aaacctggaa cggtgtcgcg gatcgcttcg atctcattaa
    46321 tgatcgaatc ttccgaacgg ctctgaataa tgcgcccttc gtgctcggta atagaacaga
    46381 aagaacagcc gccaaagcag ccgcgcataa tgttgaccga aaaacggatc atttcgtaag
    46441 ccggaatacg ggcattgcca taggccggat gtggcacgcg cttgtacggc agcgcaaaaa
    46501 cgctgtccat ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt
    46561 caccgtgttt ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc
    46621 gcgaagcatg ggcgtacagc actttatcgc ccttcacttt ctcgaaagaa ggcagcaata
    46681 cgtaggtttt ttcccacggt ttctggcgcg gtggctgcac gattacagct ttcgcttcct
    46741 gctttttcgg cgcaacaggt ttgttatccg cacacggcaa atcttcgcca tacggatgcg
    46801 ggattggggc gattttccct ggggtatcaa gacgggtgga atccacaccg ctccagccag
    46861 gcagcgcctc ttttacgata attgcggtat tacgcacatc gcggatttca ctaatcggct
    46921 cgcccatcgc cagacgatgc actacttcca ccagcggacg ttcaccgtta ccaaacatca
    46981 gcatgtcggc tttcgaatct accagaacgg aacggcgcac ggtatcggac cagtaatcat
    47041 aatgcgcggt acggcgcagg ctagcctcaa taccgccgag gatcaccggc acatctttcc
    47101 acgcctcttt acaacgctgg gtataaacca gcgtggcgcg atccggtcgc ttacccgcga
    47161 cattatctgg cgtgtaggca tcatcatgac gtaaacggcg atcggcggta tagcggttga
    47221 tcatcgaatc catgttgcca gcagtaacac caaaaaacag attcggttta cccagacgca
    47281 taaagtcgtc tttgctgctc cagtccggct gggcgatgat cccgacgcga aagccctgcg
    47341 cttccagcat acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc
    47401 cagtaaccaa aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca
    47461 tcggcaaaaa cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt
    47521 cgcgatccgg ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat
    47581 aatcaaaggc cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc
    47641 gccctcctcg ggcgagtatg aagattacgg taccggattg accaacagtt gcccaatcga
    47701 gccacggtcc gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga
    47761 aggctgaccg aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca
    47821 gcctctgtct tccgggaacg gcatcgcgcc attcacgtta cggatctgga acattacgcc
    47881 ttcaatatgg aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg
    47941 ctgcgcggtg acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc
    48001 acccagactg atatcgcgac tgcgaattgg cgaaccagcc atgatttcag ttggcagcaa
    48061 gcgcatcgga agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac
    48121 caaggtagaa accagaatac tggatggctc aaagaagccg cgaatacgat caacaatgct
    48181 cgccgcttca ccacaggtga tcgacacttc atcgccgttg ctcatatcca ccagaatctc
    48241 gcggcgctcg cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc
    48301 ctgatcgcca gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg
    48361 agagttcgac gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg
    48421 gctttgtaca ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt
    48481 gtattctggc gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc
    48541 cacaccataa tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat
    48601 tccggcaagg ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca
    48661 cagagtagct gcgttctggc gaataggcag tacgggtgcc cagtcagcgt ttggcgacat
    48721 catccgtgcc ggaccgccca tcagcgggcc tggtacctgt agcccggcca ccgtcattga
    48781 gacattttct gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg
    48841 gatagtcggc cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt
    48901 aaatgaccag tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag
    48961 tagcggcgga acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc
    49021 gcctgcacaa agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt
    49081 tgctccatgt aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac
    49141 agattgccga cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg
    49201 gcgatttttt gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg
    49261 ccatactgac tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt
    49321 cgattaagat taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc
    49381 gccgcaattg ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg
    49441 cgggttcctt ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact
    49501 tccgcaatgg tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt
    49561 aaccagtaca actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc
    49621 gtcggcggtt gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg
    49681 atatagatag cattgccgta gttttcagca tctgcaggtt tgcggcactc aactttcagg
    49741 ccgaacagtg gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta
    49801 cgcgggctga aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg
    49861 gtaataataa gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat
    49921 tgtacgtcac ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat
    49981 tgttaatcgc aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg
    50041 ttgaatacgc tgccggatga cgacttaacg tttcatccgg cgttccttgc tagcgggagg
    50101 aaacagcgcc ctccccggca tattactctt cgctatcacc gctgctggca cggcgaggag
    50161 agtcgatctc aacacgatcg atacgctgca aaccgcgcat caacgtaccg cggcgtccac
    50221 gttcgccagt gactttctgt agctcttccg gacgcagttt aattttgcgt ttcccaacat
    50281 gaatggtcag cgtgctttgc ggcggcagaa cgtacagttg cgccagacca tcctcgccac
    50341 gcgcggcttc tgccgatgga atgttgataa tcttgttgcc tttgcccttc gacagctgcg
    50401 gcagatcact taccgggaac atcaacatac ggcctgcctg agtgattgcc agcagcatat
    50461 cggaagcatc ttcaatcacc accggcggca taacatgggc attttccggt aaggtgatca
    50521 aagccttacc cgcacggtta cgcgctacca gatcgttaaa ggtgcagacg aaaccgtaac
    50581 ccgcatcgga agccatcagc agtttctgat cgtcgctttc catcagcata tggtcaacgg
    50641 tcgccccagg cggcaacgtt aatttgccgg tgagtggttc gccctgacca cgcgccgacg
    50701 gcagcgtaat cgggtcgatg gcatagctac gaccggtgga atcaacaaac actaccggtt
    50761 ggttactctt acctttcacc gccgctttga agctatcacc cgctttataa ttcaggcccg
    50821 gcgcgtcgat atcatggcct ttagcgctgc gtacccagcc catctgcgac agcacaatgg
    50881 tgacaggttc agacggcagc atgtcgtgct cgctcatcgc tttcgcttct tcgcgttcct
    50941 gcaacggcga acgacgatcg tcaccgtagg cttgcgcgtc tgcctgcagt tctttcttca
    51001 gcaggttatt cattttacgc tcggaagcca aaatgccctg caactggtcg cgctcttttt
    51061 ccagctcact ctgctcaccg cgaatcttca tctcttccag tttggcaaga tgacgcagtt
    51121 tcagttcgag gatcgcttcc gcctgggttt ccgtaaggcc aaaccgcgac atcagcgccg
    51181 gtttcggttc atcttcgtta cgaatgatct caatcacttc gtcgatgttg agaaacgcca
    51241 ccagcaaacc ttccaggata tgcaggcgct tgaggacttt ctccagacga tagttcagtc
    51301 ggcggcgcac ggtatcgcga cggaacacca gccattcgga gaggatttcc agcaggtttt
    51361 tcaccgccgg acgaccatcc agaccgatca tattgaggtt aatgcgatag ctcttttcca
    51421 gatcggtggt agcgaagagg tggttcatca cctgatccat atccacgcgg ttggaacgcg
    51481 gcacaatcac cagacgggtc gggttctcgt ggtcagattc atcgcgcaga tcgtcaacca
    51541 tcggcagctt tttgttgcgc atttgcgcag caatttgctc cagtacgcgc gcacctgaaa
    51601 cctgatgcgg taatgcgctg ataaccaccg cgccatcttc tttcttccac accgcgcgca
    51661 tacgcactga accacgtccg ttctcgtaga ttttacgaat ctcggcgcgc gaagtgataa
    51721 tttccgcttc agtcggataa tccggcccct gcacgatatc cagcagctga tcgagcgtag
    51781 ttttcggctg gtcgattaat gcgattgccg cctgagctac ttcacgcagg ttatgcggtg
    51841 gaatatcggt cgccatgccg acggcaatac cggtggtgcc gttaagcaaa atgtttggca
    51901 gacgggcagg tagcattttc ggctcctgca aagtgccgtc gaagtttggc acccagtcag
    51961 ccgtcccctg ccccagctcg ctcaatagca gctcggaata tttcgacaac cgggattcgg
    52021 tgtaacgcat tgccgcgaac gatttcggat cgtccggcgc gccccagttc ccctgaccat
    52081 caaccagcgg ataacggtaa gagaacggct gcgccatcag gaccatcgct tcataacagg
    52141 cgctatcgcc gtgcggatgg tatttaccca gtacgtcacc gacggtacgg gccgattttt
    52201 taaatttggc gctggcattc aggcccagtt cagacatcgc atacacaatg cggcgctgaa
    52261 caggtttcag accatcacca ataaacggca acgcacggtc catgatcacg tacatggagt
    52321 agtttaagta ggcgttttcc gtaaattcat gtagcgcaag gcgctctgcc atatcgctca
    52381 ttaattctga ttcctcaact tattcgccag ccttcaaagg ggcaatattg ccggagatac
    52441 tacattatct gccgcgtcga gtcacaggag atcagcgtga atagcgccgc cggacgtgct
    52501 ccgacggcga taaaggcatc aatgtgcctt gatatacaac tctttgctgt agacttgatc
    52561 ggcatagagg gcagcacaag gttgcgccca tgccacacca ctcggcatgg gggtccgctc
    52621 cgggcgattc gagaagttga ggttatgcga gtccagggaa gcctaaagca tcaaagtagt
    52681 cgactgtaag tatacggttc agcccagttt agctgtgtct ccaccagtgc cccgcgcctc
    52741 ctgccataag tgcggcttgc tcatggatat ccccgagacg gcttacggcg caccggataa
    52801 taaatttcgc gtatacatgg tattccttac cggagaaatt ataagcccaa caacaatacg
    52861 cctgtgacat tgttcgctca aacttatcgc gagtgatttg gctcacactc cggtaaagat
    52921 tccccatcac gccaacggca cataaacatc cgtttccagt cgctgctctg tcaccccttc
    52981 ggcaagattg gtgtagtgaa acagaatcgg tgctttacgc attttctcgc cgcttgcagg
    53041 cagccagtgg cgaataatgc cccatatcgt gtgtgaaata tcgtccagct cgccaacgtg
    53101 gcgggccacg gcataacgtc caccggtaag ttcaccattg ctcacaccat aacgattatc
    53161 gggaatcggt tcgctaacgc tgccgcagat atcaaagcga aacgcttccg gtgcggtggt
    53221 ggcaggggcg tcccaggcga cgccaaaagt ctggctttgg ttaacaggag aaagccccgt
    53281 ctctttacgc cacatgataa atttcgccac actgtagttg agcaactcag ggctacagcg
    53341 gtgcggcaac atcgccacag ggattgatgg gaaatcgata atgtttacat ccagtgtcag
    53401 gtttgtcatt ttgcctccct gcgaaattat tcccgggaac gcgtcccggg agcggtaaca
    53461 attacggatt tactttgcgg atctgtttga cgtcaatttc aacagaattc cagtctttat
    53521 cgacttcacc ctgaatctca accgtatctt tcggcgtcac cgtcacaccg ttccagcgtt
    53581 tgtgatcgat atcaacattg atagtaccgc tggcatcttt aaacacgtag agatcgtcag
    53641 agatgcgttc aacgatattg ccgcgcaggg ttacccaggt gtcgtcacgc agggattttg
    53701 cgctttctac agtcgttacg ctgccgttcg gcccctgaaa tcctccggcc tgactttgcg
    53761 ttaccgatgg gccagaaaaa ccgccctgct ctgctgccat caccggtgca ctgcacaggg
    53821 ccattactgc gattactgct gcgaattttt tcatgtttat tactcccttt aatgtctgtt
    53881 tccgagcatt taacaagata gtccttaaca acttcttaag ggaaaaaaat aaaatttaat
    53941 gctgtacaga gcgcgttaca acacggttta ctggcagcaa atacggttat cgcagggatg
    54001 aaaaaatgcg aattttactg atagaagatg acatgctgat tggtgacggc atcaaaactg
    54061 gccttagtaa aatgggtttt agtgtcgact ggtttacaca aggtcgtcag ggaaaagagg
    54121 cgctttatag cgcaccttat gatgcggtga tcctggattt aaccttacca ggcatggatg
    54181 gtcgcgatat tttgcgcgaa tggcgagaga aaggtcagcg tgagccggta ctgaacctga
    54241 ccgcgcgtga tgcgttagcg gaacgtgtag aagggctgcg tctgggagct gacgattatc
    54301 tgtgtaaacc ttttgcgttg atagaagtcg ccgccaagct ggaggctctg atgcgccgaa
    54361 ccaacggcca ggccagcaac gagctgcgcc acggtaacgt catgctcgac cccggcaaac
    54421 gtatcgccac gctggctggc gaacccttaa cactgaaacc aaaagaattt gccctgctgg
    54481 aattactgat gcgtaacgct ggtcgggtac tgccgcgcaa actgattgaa gagaaactgt
    54541 atacctggga cgaagaggtc accagtaatg ccgttgaagt gcatgtgcat catctgcgac
    54601 gcaaactcgg tagtgatttt attcgtaccg tgcatggtat tggctacaca ttaggtgaga
    54661 aatgaaattt acccaacgtc ttagtctgcg cgtcaggctg acgctaatct ttttaattct
    54721 ggcctcggtg acctggctgc tttccagctt tgtcgcctgg aaacaaacaa cggataacgt
    54781 cgatgaattg ttcgacaccc aactgatgct gtttgccaaa cggttaagta cgctcgatct
    54841 caacgaaatc aacgcggcgg atcgcatggc acagacgcca aataaattaa aacacggtca
    54901 tgttgatgac gatgcgctga cctttgccat ctttacccac gacggcagaa tggtccttaa
    54961 tgatggcgat aacggagaag atattcccta tagctatcaa cgggaaggtt ttgctgacgg
    55021 gcaactggtc ggtgaagacg atccttggcg ttttgtctgg atgacctcac ctgatggcaa
    55081 atatcgcatc gttgttggcc aggaatggga ataccgtgaa gacatggcgc tggcgattgt
    55141 tgccgggcaa ttgatcccgt ggctggtcgc actgccgatt atgttaatca tcatgatggt
    55201 actactgggt cgtgaactcg cgccgctgaa caaactggcg ctggcactac gtatgcgtga
    55261 ccctgactcg gaaaaaccac taaacgcgac tggcgtaccc agcgaagtgc ggccactggt
    55321 tgagtcgcta aatcaactgt tcgcccgcac acatgcgatg atggttcgag aacgacgctt
    55381 tacctccgat gcagctcacg aactacgtag cccgttaaca gcgctgaaag tgcaaaccga
    55441 agttgcgcag ctctctgacg atgatccgca ggcgcggaaa aaagcactgc tccaattaca
    55501 ttccgggatc gatcgcgcta ctcgtctggt tgatcaactg ctcacgctat cgcggctgga
    55561 ctcactggat aaccttcagg acgtcgcgga gatcccgctt gaagatctcc tacaatcgtc
    55621 ggtgatggat atttaccaca cggcgcagca ggcgaaaatt gacgtgcgac tgacactcaa
    55681 tgcccacggc atcaaacgca ccggacaacc gctattgcta agtttgttgg tgcgaaattt
    55741 gctggataac ggccccggtg tgacaccaga ggcactggcg cgaattggcg aacgcttcta
    55801 tcgcccaccc ggacaaaccg ctaccggcag tggacttggg ctatcgattg tccagcgaat
    55861 cgccaagctg catgacatga atgttgagtt tgggaatgcg gaacaaggtg gatttgaggc
    55921 gaaggtaagc tggtaacttc tctgtctatg agagccgtta aaacgactct catagatttt
    55981 actaatagca aaatataaac cgtccccaaa aaagccacca accacaaacc agacaaataa
    56041 cgagcgagaa tacatatagc caaaactcga acatttcccg cataaagagt tttcttaaga
    56101 taagaataat aagtggcgta agaagaaaaa atgctgcaat cattgcttgc aatatacgat
    56161 cttcgcctga aacggaaggt atactgcaaa tattgcctcc cgactcaagc atccagtcat
    56221 acttattgaa agcaaaaaaa gaatctatta accaataagt aagcattata tatagcaatg
    56281 cttcaaatat tgttttaatt ttctgcttta acatatatca gtacgctcat aaaaatccat
    56341 ttataattat caacatgaca cgttgatatt ttcatctttt acttattcaa agccatattt
    56401 tcaatcccca aaaaaaccga accaaacgtg taaattattc cggtgcgagt aagaattatg
    56461 aggaatggct atcagtattg tcattttcag aaaatattta tcctgcatcg gtgagtcaga
    56521 gctagatcag acttttgcta aattcgcaaa agactttgca cattttgcta atttcacagt
    56581 accgctctgt gacgtactat agtcgacaaa cgtctcacct tgagattaaa aaatgagcaa
    56641 catcctgatt atcaacggcg cgaaaaaatt cgcccactcc aatggtcaac tgaacgacac
    56701 cctgaccgaa gtcgcggatg gcacactgcg cgacctcggg catgatgtcc gcatcgttcg
    56761 cgccgacagc gactacaatg tcaaagcgga agtacaaaac tttctctggg ctgatgtggt
    56821 gatctggcag atgccaggct ggtggatggg cgcgccgtgg acagtgaaaa aatatattga
    56881 tgatgtattc accgaaggtc acgggactct gtatgccagc gatggtcgta cccgcaaaga
    56941 tccgtcgaaa aaatacggtt ccggcagcct ggtacagggc aaaaaatata tgctttctct
    57001 aacctggaac gcaccaatgg aagccttcac cgaaaaagat cagttcttcc acggcgttgg
    57061 cgttgacggt gtgtatctgc cgttccataa agcaaaccaa ttcctcggta tggaaccgct
    57121 gccgacattt atcgctaatg acgtgataaa aatgcctgat gttccccgct atactgaaga
    57181 atatcgcaag catcttgtgg aaatttttgg ttaactagag ctcaggcttt agaaggagtt
    57241 aaccatgctt accgtaatcg cagaaatccg tactcgtcct ggtcaacatc accgtcaggc
    57301 ggtattggat cagtttgcta aaatcgttcc aaccgtactg aaagaagaag gttgccacgg
    57361 ctatgcgcca atggtggatt gcgctgctgg cgtgagtttc cagtctatgg caccggattc
    57421 tatcgtgatg attgagcagt gggaaagcat cgcgcacctt gaagcgcatc tgcaaacccc
    57481 gcacatgaag gcgtacagcg aagccgtaaa aggcgacgtg ctggagatga atatccgtat
    57541 tctgcagcca gggatttaat cctgccttgt ttgcccggcc atcctgaccg ggcaatgttc
    57601 tttcctttaa acctcaatct ccgccatgtc gcctttctct tgcaaccagt tgcggcgatc
    57661 ttccgagcgt ttcttcgcca gcagcatatc catcatcgcg tcagtacgct gatcgtcttc
    57721 atcatcgata gtcaactgca ccagacggcg agtgttcgga tcaagcgtgg tttcacgcaa
    57781 ttgcatcggg ttcatttccc ccagaccttt aaaacgctgg acgttcggct tgcctttctt
    57841 gcgttttaat tgctcaagta cgccctcttt ctcttcttcc gtcagcgcgt aataaacctc
    57901 tttcccgaga tcaatacggt agagcggtgg cagtgcgacg taaacgtgac cgtgtttcac
    57961 caacgcgcgg aagtgtttta cgaacaaagc gcagagcagc gtggcaatgt gcagaccatc
    58021 agagtccgca tccgcgagga tacagatttt gccataacga agctggctca gatcgtcgct
    58081 gtcaggatcg ataccgatcg ctaccgaaat atcatgcact tcctgcgagg ccagcacttc
    58141 gtcggaagaa acttcccagg tgttaaggat cttacctttc agcggcatga tcgcctgata
    58201 ttcacgatcg cgcgcctgct tggcagatcc gcctgcggag tcaccttcca ctaggaacag
    58261 ttcggtacgg ttaaggccct gcgcggtaca atcagccagt ttgccaggca gcgccgggcc
    58321 gctggtcagc tttttacgca ccactttttt agccgcacgc atacgacgct gggcgctgga
    58381 aatcgccatc tctgccagca actccgccgc ctgaacgttc tggttcagcc acaggatgaa
    58441 ggcatctttc accacgcccg aaacgaatgc cgcacactga cgcgaagaga gacgctcttt
    58501 ggtctgcccg gcaaactgcg gatcctgcat tttaaccgac agaacatagg cgcagcgatc
    58561 ccagatatct tccgccgaca gcttcacgcc gcgcggcaga atgttgcggt attcacagaa
    58621 ctcacgcatc gcgtccaaca ggccctgacg cagaccatta acatgggtac cgccctgcat
    58681 cgttgggata aggttgacgt agctttcggt cagcagttca ccgccttccg gcagccacag
    58741 tagcgcccag tccacagctt cagtatcgcc agcgaaatta ccgataaacg gtttttctgg
    58801 cagcgtcggt aaaccgttta ccgcttccgc caggtaatca ttcagaccgt cctgatagca
    58861 ccagcgctgt tcggtgttgt tgatctcatc tttaaaggta atctcaacgc cggggcacag
    58921 tactgctttg gccttcagca catgcgtcag gcgtgaaaca gaaaaacgcg ggctgtcaaa
    58981 gaaggtttca tccggccaga agtgcacgct ggtaccggta ttgcgtttac cgcaagtgcc
    59041 gacaacctgt aaatcctgca ccttttcgcc attttcaaag gcgatgttat aaacctgacc
    59101 atcgcggcgc acgttaactt ctacgcgctt cgacaaggcg ttaactaccg aaatccccac
    59161 gccatgcagg ccgccagaga actggtaatt tttgttagag aatttaccgc ctgcatgcag
    59221 acggcaaaga atcagttcaa ccgccggtac tccttcttcc gggtgaatat ccaccggcat
    59281 cccgcgccca tcgtcaatca cttctaacga ctggtcagca tgtaagatta catccacgcg
    59341 ttttgcgtga cccgccagtg cttcatcgac actgttatca ataacttctt gccccaaatg
    59401 gttagggcga gtggtatcgg tatacatccc cggacggcgg cgaaccggct caagcccggt
    59461 gagtacctca atggcatcag cgttataagt ttgcgtcatg gtttaagtta gcaattcgag
    59521 ttgatcgtca gagatggtgc agaccaagaa aatcgacgat cgggttgaaa taatcttcga
    59581 agcccgtgaa tgcgtggttg ccgccttcta tgacagtctg gcggcaggaa gcgtagtacg
    59641 ccaccgcctg gcggtaatcc agcacttcat ctcccgtctg ttgcagcagc cagatcaaat
    59701 ccggcgcttc cagcgggtca atctgcatga ctttgagatc gtaaatatgg cgtgactcta
    59761 gcacatattg ctgcccggtg taggggttct cgttctgacc gagatagtcc gtcagcaatt
    59821 caaacgggcg taccgccggg tttaccacca ctgcgggcag cataaaacat tgcgacaacc
    59881 aggtggcgta atatcccccc agtgacgaac cgacaatacc cagcgaatcg ccgccatgtt
    59941 ccaggacaat ggattccagc agttctgccg cgtcggaagg atacggcggc aactgcggaa
    60001 tgatcatctc aacgtcaggg tgatgttccg ccagccagtt ttttaacaag ctcgcttttg
    60061 cagagcgcgg cgagctgttg aaaccgtgta aataaagaag cgtagacatc agtagccttc
    60121 tgaagcggta tcaggttgga aacgtgtgtc cgccaggcga tgcacctcgg tggtcagcgt
    60181 gccatcagca tgtaactcga gagtacgcca gccgggcgcg atagtatcca gcgtaaagtt
    60241 ggaacagtgc ggcttaaact gcacacaggt cgacggcgtt gccagcaggc ggcgaccgtt
    60301 ccagtcgaga tccagctcct gatgaatatg accgcacagc aagtatttga cgtgcggaaa
    60361 cttcgccagc acggtatcca gttcgcccgc gttacgcaga ctgtgttgat cgagccaact
    60421 acaacccgca ggtagcggat gatgatgcag cagcagcaac gtatggcgtt ctggcgcatc
    60481 ggccagttta cgttccagcc actcaagctg aaactcgctc agctcaccgt gcggcacgcc
    60541 aaacacctgg ctatccagca acaggatttg ccattgctca ccaataaaca cccgcttcgc
    60601 cggggagata cccgcatcct gtaacgcgct gtacatcgcg ggctggaaat cgtggttgcc
    60661 cggcagccag acgcagggcg cacgaaaact tgcgatgcct tcagcgaaat gctgataggc
    60721 cgcagaggat tgatcctgcg ctaaatcacc tgtcgcgaca atcaggtcga attcgtgctg
    60781 gtgtggacga atcgcctcca gcaccgcctg gtagctttcc caggtgttta cccctaacag
    60841 ggcttcgtgc ttttgtgcaa acaggtgagt atcggtaatt tgtaaaatcc ttcctctggc
    60901 ctcaccagcc agaggaaggg ttaacaggct ttccaaatgg tgtccttagg tttcacgacg
    60961 ctagtaaacc ggaatcgcca tcgctccatg tgctaaacag tatcgcaacc agtccgctaa
    61021 aaactggtta atttgatgct tttcgtcgcg ttgatgcaac tttttattag gataatcata
    61081 ccgcgctttg aagcgaaaaa tctgctggct tgaacacact tcagccacca tcgcgtcatg
    61141 atacagacgc accgtcattg acggaaggct ccagtaactg atcgcgggcg cagtctgttc
    61201 tattgtcact agggtagtgt atcgggtcga ttccacaatc gtcagccgat attgtgcgtt
    61261 tgccacctga tagcttacag tttcgccggg tgcgtcattg cgcggtaaca aacggcgcaa
    61321 ttgtgaaaag ttcatctcgc acaggcgcat catttcagga aagtcaggtg tgtaacgctt
    61381 catttatgcc cactcatttt ttaacgcttg atgatgcagc tgcagccatt gcaaagcgat
    61441 gaccgacgct gcgttgtcga ttttcccctc ttctacccac tggtatgcct gttcccggct
    61501 taccacatga acgcgaatat cttcgttttc atcagccaga ccatgaatac cgcttgcggt
    61561 cgtggcgtcc acttcgccca ccataattga cgaacgctca ctggtgcccc ccgggcttgc
    61621 caggaaactt aacaccggtt tggtccgttt gactatcagt cccgcctctt caatcgcttc
    61681 gcgacgggca acatcttcca cactttcacc ctcttcaatc atcccggcaa ccatctccag
    61741 tagccagggg gtttcgctgg tgtcgtacgc ggcaatccga atctgctcaa tcagcacaac
    61801 ttcatcacgc actgggtcaa agggtagcaa gactgcggcg tgaccgcgct caaaaatttc
    61861 ccgccgtacc tcatgactca tttgcccgtt gaatagacga tgacgaaatc tataaagatc
    61921 taatgaaaaa aagccgcgat aaagtgtttc tcgtgcaata atttctacat cgtttttgcc
    61981 aaatgtaacg ggcaggttgt ctggcttaag cattgttaat gtcctagcac taatagtgaa
    62041 ttaaatgtga atttcagcga cgtttgactg ccgtttgagc agtcatatgt taaattgagg
    62101 cacattaacg ccctatggca cgtaacgcca accttttgcg gtagcggctt ctgctagaat
    62161 ccgcaataat tttacagttt gatcgcgcta aatactgctt caccacaagg aatgcaaatg
    62221 aagaaattgc tccccattct tatcggcctg agcctttctg ggttcagttc gttgagccag
    62281 gccgagaacc tgatgcaagt ttatcagcaa gcacgcctta gtaacccgga attgcgtaag
    62341 tctgccgccg atcgtgatgc tgcctttgaa aaaattaatg aagcgcgcag tccattactg
    62401 ccacagctag gtttaggtgc agattacacc tatagcaacg gctaccgcga cgcgaacggc
    62461 atcaactcga acgcgaccag tgcgtccctg cagttaactc aatccatttt tgatatgtcg
    62521 aaatggcgtg cgttaacgct gcaggaaaaa gcagcaggga ttcaggacgt cacatatcag
    62581 accgatcagc aaaccttgat cctcaacacc gcgaccgctt atttcaacgt gttgaatgct
    62641 attgacgttc tttcctatac acaggcacaa aaagaagcga tctaccgtca attagatcaa
    62701 accacccaac gttttaacgt gggcctggta gcgatcaccg acgtgcagaa cgcccgcgcg
    62761 cagtacgata ccgtgctggc gaacgaagtg accgcacgta ataaccttga taacgcggta
    62821 gagcagctgc gccagatcac cggtaactac tatccggaac tggcggcgct gaatgtcgaa
    62881 aactttaaaa ccgacaaacc acagccggtt aacgcgctgc tgaaagaagc cgaaaaacgc
    62941 aacctgtcgc tgttacaggc acgcttgagc caggacctgg agcgcgagca aattcgccag
    63001 gcgcaggatg gtcacttacc gactctggat ttaacggctt ctaccgggat ttctgacacc
    63061 tcttatagcg gttcgaaaac ccgtggtgcc gctggtaccc agtatgacga tagcaatatg
    63121 ggccagaaca aagttggcct gagcttctcg ctgccgattt atcagggcgg aatggttaac
    63181 tcgcaggtga aacaggcaca gtacaacttt gttggtgcca gtgagcaact ggaaagcgca
    63241 catcgtagcg tcgtgcaaac cgtacgttcc tccttcaaca acattaatgc ttctatcagt
    63301 agtattaacg cctacaaaca agccgtagtt tccgcgcaaa gctcattaga cgcgatggaa
    63361 gcgggctact cggtcggtac gcgtaccatt gttgatgtgt tggatgcgac caccacgctg
    63421 tacaacgcca agcaagagct ggcgaatgcg cgttataact acctgattaa tcagctgaat
    63481 attaagtcag ccctgggtac gttgaacgag caggatttgc tggcactgaa caatgcgctg
    63541 agcaaaccgg tttccactaa tccggaaaac gttgccccgc aaacgccgga acagaatgct
    63601 attgctgatg gttatgcgcc tgatagcccg gcacccgtcg ttcagcaaac atccgcacgc
    63661 actaccacca gtaacggtca taaccctttc cgtaactgat gacgacgacg gggcttcggc
    63721 cccgtctgaa cgtaaggcaa cgtaaagata cgggttatct gccgcattct tcccccttct
    63781 cgcttcaatt tcgaccagcc atcctctatt ctgatgggta tttaccactg gtcccggaag
    63841 acaaaaatga aacggacaaa atccatacgc cacgcatcgt tccgcaaaaa ctggagcgca
    63901 cgccatctga caccagtcgc tctcgcggtt gccactgttt ttatgctggc tggctgtgaa
    63961 aagagtgatg aaacagtgtc tctctatcaa aatgctgacg actgttcagc tgcaaaccca
    64021 ggcaaaagcg ccgaatgtac caccgcgtac aacaatgcgc tgaaagaagc cgaacgtact
    64081 gcgccgaaat acgccacccg tgaagactgt gttgctgaat ttggtgaagg tcagtgtcag
    64141 caagcaccag cccaggctgg catggcacca gaaaaccagg cgcaggctca gcaatccagc
    64201 gggagtttct ggatgccgct gatggccggt tacatgatgg ggcgtctgat gggcggcggc
    64261 gcgggatttg cacagcagcc gctgttctcc tcgaaaaacc cggccagtcc ggcttacggt
    64321 aaatataccg acgcgacggg taaaaactat ggcgcagccc agccaggccg caccatgacc
    64381 gtaccgaaga cggcaatggc accaaaaccg gcgaccacca ctaccgttac ccgtggcggt
    64441 tttggtgaat ctgttgccaa acaaagcact atgcagcgta gcgcaaccgg cacctcttct
    64501 cgttcaatgg gtggctgata ccgatggaaa gagtcagtat taccgagcgc ccggactggc
    64561 gtgagaaagc ccacgaatac ggtttcaatt ttcacaccat gtacggcgag ccgtactggt
    64621 gtgaagatgc ttactacaag ttgaccctcg cccaggttga aaagctggaa gaagtcaccg
    64681 ccgaactgca ccagatgtgc ctgaaagtgg tggaaaaagt gatcgccagc gatgagctga
    64741 tgaccaaatt ccgcatacca aaacacacct ggagttttgt gcgtcaatca tggctgactc
    64801 accagccatc gctttattcg cgtcttgatc tggcgtggga tggcactggc gaacctaaac
    64861 ttctggaaaa taacgccgat acgccaacgt cactatacga ggcggcgttc tttcagtgga
    64921 tctggctgga agatcagctt aacgccggta acttgtcgga gggcagcgac cagtttaaca
    64981 gtctgcaaga aaaactgatc gatcgcttcg ttgagctgcg tgaacagtat ggcttccagt
    65041 tgctgcatct cacctgctgt cgcgacacgg tggaagatcg cggaaccatt cagtatttgc
    65101 aggactgcgc aaccgaagct gaaatcgcca ccgagttcct ctacatcgac gagataggtt
    65161 taggcgaaaa aggccagttc acggatttac aggatcaggt gatttctaac ctgttcaagc
    65221 tttatccgtg ggaatttatg ctgcgcgaaa tgttctccac caagctggaa gatgctggcg
    65281 tacgctggct ggaaccggcg tggaagagca ttatctccaa caaggcactt ctaccgctac
    65341 tgtgggagat gttcccgaat cacccgaacc tgctgcccgc ttattttgcg gaagatgatc
    65401 atccgcaaat ggaaaaatat gtggttaaac cgatcttctc ccgtgaaggc gcaaacgtgt
    65461 cgatcattga gaacggcaaa accattgaag cagcggaagg tccgtatggc gaagaaggga
    65521 tgattgttca gcaattccac ccgttaccga aattcggcga cagctatatg ctgattggta
    65581 gctggctggt gaacgatcaa cctgccggaa ttggcattcg tgaagaccgt gcgttgatca
    65641 cccaggatat gtctcggttt tatccacata tttttgttga ataagccacg ataccggatg
    65701 gcactcgcca tccggtaatt gttagcctat ctgcaccgac agcatactta aactgcccat
    65761 ttctatacca tcgaccggaa tggtaattgg ctcctgccca tcccacacac ctaacacata
    65821 caacaacggc agatagtgct ctggcgttgg gttcgataac gcgccacctt catggtcgag
    65881 ataattcacc agaggatgct gctctaccgg cccttgccac gtcagattcg ctttcacata
    65941 ctcattaaac gacgtcgccc acggatacgg tgaactatca ccgtgccact tcactgtgcg
    66001 caggttatgc accacgttac cgctggcgac caacattatt ccttcatcac gcagcgccgc
    66061 gagtttgcgc cccatttcga aatgccaggc gacaggtttg ctactgtcga tactcaactg
    66121 caccataggg atatcggcgt caggatacat cttaatcagc acgccccacg agccgtggtc
    66181 aaagccccag gcttctttat ccagcgtcac cgggatcggc gctaacagct caaccagacg
    66241 ctgtgccagc gcaggcgaac ccggagcagg ataatgcgta tcgtacagcg cctgcgggaa
    66301 gccaccaaag tcatgaatcg tgggcggcgt ctccatcgcg gtcactcctg ttccacgggt
    66361 aaaccagtga gccgaaacca ccacaatcgc ttgcgggcgt ggcaatgtca tccccaactt
    66421 ctgccagctg cgggtataca aattatcttc cagcacgttc atcggactac cgtgacctaa
    66481 aaacaatgct ggcatacgtg ttgaagacat gatgatatcc ttaactaaag gtgtcatttt
    66541 gatatcctca caatacgctt gttcggcgga gtaagaaccc ggataacaat gatgatgatc
    66601 atcagttatt ttgacgatct gcctgaaggt gaagatttat aaggagttgt cgatgtcagt
    66661 acctctcatt ctgaccatac tggcgggggc agccacgttt attggcgcgt ttctcggcgt
    66721 tctcggacaa aaaccctcga accgcttact ggcgttttcg ctaggttttg cggcggggat
    66781 catgttgctc atctcattaa tggaaatgct tcctgccgca ctagcagctg aaggaatgtc
    66841 gccggtgttg ggttatggaa tgtttatctt cggtctgctt ggctactttg gcctggaccg
    66901 catgttgccg catgcgcatc cgcaggattt aatgcaaaaa tcggtgcagc tgttgccaaa
    66961 atcgatcaag cgcacagcca ttctgctcac tctcggcatc agtctgcata acttcccgga
    67021 agggattgcc acctttgtca cggcgagcag caacctggag ctgggatttg gcatcgcact
    67081 ggccgtcgcg ttgcacaata tccctgaagg tctggcagtg gcaggcccgg tttatgcggc
    67141 aacggggtct aaacgcaccg cgattctgtg ggcggggatt tctggactag cagaaattct
    67201 tggtggtgtg ctggcgtggt taatcctcgg tagcatgatt tccccggtgg tcatggcggc
    67261 aatcatggcg gcggttgcag gaattatggt ggcgctctcg gttgatgaat taatgccgct
    67321 cgccaaagag attgacccta ataataaccc cagctatggc gtactatgtg gaatgtcagt
    67381 gatgggattc agtttagtgc tgctacaaac ggcgggaatt ggttaaaaac aacatcagaa
    67441 gttgtccagc acatggacaa cttctgccag cttcacgaaa gccttattct accttttcgg
    67501 ctgtctcttc tctcgtactg tttaagtatt tgttccgcgc ttcaagcagt gcgtctctgt
    67561 cctggcgaaa cgcccgacaa taacgtttca aatgcaggaa ataacccacc acaaccagag
    67621 caatcaatac aatccagtac caggcgataa acatcctttt acccttaata tttaacaata
    67681 ctaaacattt agcgcataaa tttcacatat cctttttcgg atatattcat atggtcgcat
    67741 aataacggaa caattatatg attttgatca taatgaaaaa gaatgcctga tattaatgaa
    67801 attttctcat agaaagagaa gcggaattaa catcttgcat agcagggcaa aataaaaacc
    67861 gaagccagct acaacttcgg tttcttgatt aaggcagtaa ttaagcagcg gttttcagct
    67921 ggctttacgc tcatgtgcct gacggtatgc caccaggtct tcaatagtca cgagcgccat
    67981 attgtgttta ttggcaaact caatgcactc tggtgcacgc gccatcgtgc catcgtcatt
    68041 agtcagctca cacagcacac cagccggttt aaagcctgcc agcgtcatca aatcaatagt
    68101 cgcttcagta tgaccgccac gcgtcagtac accacctgcc tgcgcgcgaa gtgggaaaac
    68161 gtggcccggg cgattcagat cagaaggttt tgcgccatcg gcaatcgctg cgcgaacggt
    68221 cgtaatacga tcagcggcag aaacaccggt agtcacacct tcagccgctt caatggtcac
    68281 ggtaaaaccg gtgccatagg cgctggtgtt attttctacc atcattggca gatcaagttg
    68341 tttacggcga tcttcagtga tgcacaggca aacaataccg ctaccgtggc gaatggtcag
    68401 cgccatctgc tcaacggtca tggtttctgc cgggaagatc atatcgcctt cgttttcacg
    68461 gtcttcatca tcaagcacca ttacaccgcg tccttcacgc agcgcagcca gtgcattttc
    68521 aacacgttcg aaaggcgtac caaaagagga aagtagcgtc tgattcatgg taaaaaaacc
    68581 tcactaaaat tatggttacc agaatcaggg cagtcttagg agtggcggca tatagccaaa
    68641 ataacgtgag cgggtccatg cccgacagaa tcgttactct ctcccatccg gactctaacc
    68701 gtcggccccg gaattacacc ggatctgctg tcctttgagt tcgcacccaa agcgctcgcg
    68761 ggctttcaac tgagttgatt taccgccggt ggggaatttc gccccgccct gagaataagc
    68821 ggattcacta taacgctaat gattagcggc agcaacgcat agcttcacat aattctggtt
    68881 tatgacttac ccttatcgca ctacaatggt actcaacacc tatcagtaca gggaaactac
    68941 aatgattgac ccgaaaaaaa ttgagcaaat cgctcgccag gttcacgaat caatgcctaa
    69001 aggaatcagg gagttcgggg aagatgtgga gaaaaaaatc cgccaaaccc tacaagctca
    69061 gctgacgcgc ctcgaccttg taagccgtga agagttcgac gtccaaacgc aagtcctgtt
    69121 acggactcgt gagaaactgg cgctgcttga acaaagaatc agcgaactgg aaaatcgcag
    69181 tactgagatc aaaaagcagc cagatccaga gactctcccc ccaacgctgt aaacatttgt
    69241 ctttaatgca ctgtaaatta tgaattaatc ctctaattta cagtgttttc tattacatta
    69301 tcgcttaatc ccattcagag tatcatcaca aaaatgaatg attgcaattg aaaatataat
    69361 tgttatgtat tctttaagtt ttacttaatc gatatttaga ctggccccc
//
LOCUS       DAEEGA010000008        67100 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEEGA010000008 DAEEGA010000000
VERSION     DAEEGA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67100
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.008"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="KLH46_000537"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="KLH46_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBE0377352.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="KLH46_000538"
     CDS             complement(1150..1236)
                     /locus_tag="KLH46_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBE0377353.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="KLH46_000539"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="KLH46_000539"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBE0377354.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="KLH46_000540"
     CDS             complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="KLH46_000540"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBE0377355.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3585..3629
                     /locus_tag="KLH46_000541"
     CDS             3585..3629
                     /locus_tag="KLH46_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377356.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="KLH46_000542"
     CDS             complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="KLH46_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBE0377357.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="KLH46_000543"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="KLH46_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBE0377358.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="KLH46_000544"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="KLH46_000544"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBE0377359.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="KLH46_000545"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="KLH46_000545"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBE0377360.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="KLH46_000546"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="KLH46_000546"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBE0377361.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="KLH46_000547"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="KLH46_000547"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBE0377362.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="KLH46_000548"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="KLH46_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBE0377363.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="KLH46_000549"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="KLH46_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBE0377364.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="KLH46_000550"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="KLH46_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBE0377365.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPSSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="KLH46_000551"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="KLH46_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBE0377366.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="KLH46_000552"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="KLH46_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBE0377367.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="KLH46_000553"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="KLH46_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBE0377368.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="KLH46_000554"
     CDS             complement(16981..17745)
                     /locus_tag="KLH46_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0377369.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="KLH46_000555"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="KLH46_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBE0377370.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQR
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="KLH46_000556"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="KLH46_000556"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBE0377371.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="KLH46_000557"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="KLH46_000557"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBE0377372.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSLAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="KLH46_000558"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="KLH46_000558"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBE0377373.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="KLH46_000559"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="KLH46_000559"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBE0377374.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="KLH46_000560"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="KLH46_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBE0377375.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSSEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHTILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="KLH46_000561"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="KLH46_000561"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBE0377376.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="KLH46_000562"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="KLH46_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBE0377377.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="KLH46_000563"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="KLH46_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBE0377378.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="KLH46_000564"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="KLH46_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBE0377379.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="KLH46_000565"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="KLH46_000565"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBE0377380.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="KLH46_000566"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="KLH46_000566"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBE0377381.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="KLH46_000567"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="KLH46_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBE0377382.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGTIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="KLH46_000568"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="KLH46_000568"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBE0377383.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="KLH46_000569"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="KLH46_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBE0377384.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39849)
                     /gene="kefC"
                     /locus_tag="KLH46_000570"
                     /pseudo
     CDS             complement(37988..39849)
                     /gene="kefC"
                     /locus_tag="KLH46_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
     gene            complement(39842..40372)
                     /gene="kefF"
                     /locus_tag="KLH46_000571"
     CDS             complement(39842..40372)
                     /gene="kefF"
                     /locus_tag="KLH46_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBE0377385.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40420..41814)
                     /locus_tag="KLH46_000572"
     CDS             complement(40420..41814)
                     /locus_tag="KLH46_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0377386.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41873..42160)
                     /gene="fixX"
                     /locus_tag="KLH46_000573"
     CDS             complement(41873..42160)
                     /gene="fixX"
                     /locus_tag="KLH46_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBE0377387.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42157..43443)
                     /locus_tag="KLH46_000574"
     CDS             complement(42157..43443)
                     /locus_tag="KLH46_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE0377388.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43494..44435)
                     /locus_tag="KLH46_000575"
                     /pseudo
     CDS             complement(43494..44435)
                     /locus_tag="KLH46_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44450..45220)
                     /locus_tag="KLH46_000576"
     CDS             complement(44450..45220)
                     /locus_tag="KLH46_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBE0377389.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45692..47206
                     /gene="caiT"
                     /locus_tag="KLH46_000577"
     CDS             45692..47206
                     /gene="caiT"
                     /locus_tag="KLH46_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBE0377390.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47237..48379
                     /gene="caiA"
                     /locus_tag="KLH46_000578"
     CDS             47237..48379
                     /gene="caiA"
                     /locus_tag="KLH46_000578"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBE0377391.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48508..49725
                     /gene="caiB"
                     /locus_tag="KLH46_000579"
     CDS             48508..49725
                     /gene="caiB"
                     /locus_tag="KLH46_000579"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBE0377392.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49799..51352
                     /gene="caiC"
                     /locus_tag="KLH46_000580"
     CDS             49799..51352
                     /gene="caiC"
                     /locus_tag="KLH46_000580"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBE0377393.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51461..52246
                     /gene="caiD"
                     /locus_tag="KLH46_000581"
     CDS             51461..52246
                     /gene="caiD"
                     /locus_tag="KLH46_000581"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBE0377394.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52252..52842
                     /gene="caiE"
                     /locus_tag="KLH46_000582"
     CDS             52252..52842
                     /gene="caiE"
                     /locus_tag="KLH46_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBE0377395.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52928..53323)
                     /gene="caiF"
                     /locus_tag="KLH46_000583"
     CDS             complement(52928..53323)
                     /gene="caiF"
                     /locus_tag="KLH46_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBE0377396.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53585..56806)
                     /gene="carB"
                     /locus_tag="KLH46_000584"
     CDS             complement(53585..56806)
                     /gene="carB"
                     /locus_tag="KLH46_000584"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBE0377397.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56824..57972)
                     /gene="carA"
                     /locus_tag="KLH46_000585"
     CDS             complement(56824..57972)
                     /gene="carA"
                     /locus_tag="KLH46_000585"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBE0377398.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58428..59249)
                     /gene="dapB"
                     /locus_tag="KLH46_000586"
     CDS             complement(58428..59249)
                     /gene="dapB"
                     /locus_tag="KLH46_000586"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBE0377399.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59416..60330)
                     /gene="rihC"
                     /locus_tag="KLH46_000587"
     CDS             complement(59416..60330)
                     /gene="rihC"
                     /locus_tag="KLH46_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBE0377400.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60396..61346)
                     /gene="ispH"
                     /locus_tag="KLH46_000588"
                     /gene_synonym="lytB"
     CDS             complement(60396..61346)
                     /gene="ispH"
                     /locus_tag="KLH46_000588"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBE0377401.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61348..61797)
                     /gene="fkpB"
                     /locus_tag="KLH46_000589"
     CDS             complement(61348..61797)
                     /gene="fkpB"
                     /locus_tag="KLH46_000589"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBE0377402.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61884..62378)
                     /gene="lspA"
                     /locus_tag="KLH46_000590"
     CDS             complement(61884..62378)
                     /gene="lspA"
                     /locus_tag="KLH46_000590"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBE0377403.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62378..65194)
                     /gene="ileS"
                     /locus_tag="KLH46_000591"
     CDS             complement(62378..65194)
                     /gene="ileS"
                     /locus_tag="KLH46_000591"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBE0377404.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65237..66178)
                     /gene="ribF"
                     /locus_tag="KLH46_000592"
     CDS             complement(65237..66178)
                     /gene="ribF"
                     /locus_tag="KLH46_000592"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBE0377405.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66186..66404)
                     /locus_tag="KLH46_000593"
     CDS             complement(66186..66404)
                     /locus_tag="KLH46_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBE0377406.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66507..66770
                     /gene="rpsT"
                     /locus_tag="KLH46_000594"
     CDS             66507..66770
                     /gene="rpsT"
                     /locus_tag="KLH46_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBE0377407.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16291 a        18257 c        17266 g        15286 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggattagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcagcgt
      301 gacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 gcaccgccgc ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg
     3361 catcgtaaat atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat
     3421 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
     3481 tttgacggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
     3541 ccagagacat aaacgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata
     3601 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
     3661 caatttctct atttttttaa ttaatcctaa aatagagagt ccacacattc agaataaaat
     3721 tctacattca ataaaaaatg cagaataaac cagacattca tgtctggcct attctgcatt
     3781 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
     3841 cccggcagct tcatgccaag agagataaca ggttctgctg ttttgtttta acggcagcgg
     3901 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
     3961 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
     4021 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
     4081 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
     4141 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
     4201 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
     4261 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
     4321 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
     4381 aagatgaaat acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg
     4441 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
     4501 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
     4561 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatattttg
     4621 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
     4681 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
     4741 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
     4801 tttatcatgc atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat
     4861 acgggttctg ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat
     4921 aatccataat gtttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgatcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caatgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggc ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cggtctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc gcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacgga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tagcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccgactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaaccgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaagacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ctggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctagtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgatgaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agttcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagccacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggaac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggttgaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cactatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgcgga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca ggtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatgtt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtac aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagcccca
    39721 cgggccaata atgcagccgg cgatcaggta gccaagtacc gatcccagac caagacgtac
    39781 cgcaatgggt acaatcagcg ccgccgatcc gaggtagatc agcgcctgaa ccagcgtatg
    39841 actatccatg atgggcctcc tgccattcca gcagacgttg cttatagtgg cgcgcctgcc
    39901 cttcgagggt ttcgtcgtca caaataaagg tgcagtgcat ggcaaacggt ggcagccagt
    39961 tcagcccgca gtagattgcc gtcgcctgta gcggctgcga cagcacatca aagcccggat
    40021 gcgcaccaat ttcaaaatgg ctttccccgc cgccggtcgt caccgcccac agcaaatgtt
    40081 tgccatgcag cgccgtgccg ccgtgaccgt aagcccagcc gtgcgacaaa actttatcga
    40141 tccaaagttt aagaagcgga ggaatgctgt accactgcat cgggtgctgc cagacgatca
    40201 gatcggcgcg agagagcgcc tcctgctcgg cggcaatatc gatattgaag tcaggataga
    40261 gttgataaag agagcgaatt tcgacgcctt ccagcgtcct tgcctgttca agcatccgtt
    40321 tattcgcatg ggaatgatgc ggatacggat gcgcataaat gatgaggatc atagttatta
    40381 gcccgcgtgt ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    40441 agtcaataat attgattcag aaaagagaga aatctgaaca aataatccca ttctctgccc
    40501 gcggatcgtc atattgctgg tctgcgccag tgacattcct cgagtctccg gggcaaacgc
    40561 tacggaaatc aacaagccaa acagcgagat acccgccccc attagcatcg tgttactgat
    40621 accgtaatta ttgataaata tcggcagtgc ccaggtcgaa acaatggtgc caatacgact
    40681 taaggacata atcacgccca cggcagaggc gcggatatcc gtcgggaaga gttcattagg
    40741 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    40801 cactaccagc cagatcccca tatccgggat tagccccaaa accgccagcg ccagcgtcat
    40861 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttagcc acagcatcgg
    40921 cggaatacag ccgagcataa agaacaggct aatcaccaca ttccctagtg ccgcgttttt
    40981 gccaaccccc aatcccaacg aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    41041 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    41101 attaaacaga tcgcgaaaac gggtttgctg cggctgctct tcatcgaaag ccaccggttc
    41161 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    41221 gcggggagat tctggcagtt cgaatcgacc aatcaaaatc aacaaacaag ggatcgccgc
    41281 gctacccagc atccagcgcc aaccaccttc cacatcataa agccagtagc cgaccagatc
    41341 ggcgcaggtc gcgccgacat accacatcgc ggcaataaag ctgatggaaa acgcccgctg
    41401 acgggtactg gagaactcgg tgatcatcga agtggcgatg ggataatctg caccgatgac
    41461 aatgccgatg agtacccgca tcaccaacag ttcgacgggg gatgaaacac acatcgtcgc
    41521 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtccgacttt
    41581 atcggagata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    41641 cagcaagcca atccagtcag cgtccagttt cagcgccgga gtcagttgct ccagcgccac
    41701 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    41761 gcggcggtga atagaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    41821 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    41881 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    41941 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    42001 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagcacct
    42061 gtttatcagc atcggccttc acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    42121 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    42181 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    42241 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    42301 ttccgggttg ccgctaaaca tgcgtgggtt atcaaggaac gccggtagtt tctggtacat
    42361 gcgcatatcg cgcagcgggc cactctccag atgctgacga tattccgcga gtttttgctt
    42421 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcagctt ccccggcggc
    42481 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    42541 accggcaatc aatacgccgt caccgaccag ctccggcagc atgttgatgc ctgcttccgg
    42601 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg caacggccgg
    42661 atgctgtttg aaatcttcca gcatttgcgg caccgatttt ttcgcgtcat gcagatgatg
    42721 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    42781 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    42841 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    42901 cacatccgtc ggtttaacgc gttttgccat ccccaacttt tcggcaagga tggagttcac
    42961 cccatcagca aggagcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    43021 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    43081 cgcttcttcg gcctgctcca tcaaccaggc atcaaattta ctgcgcaaaa cggagtaaga
    43141 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    43201 cgtcataaac gcgagttttt catgggtgat caggcgttct acgggggcgg aatcggcgaa
    43261 acctggaata atgtgttcca gactgtgggc atagagacgc ccgccagtaa cgttcttggc
    43321 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    43381 tgcggcaacc gaaccggcaa gccctgcacc gacgatgatg gcgtcaaaga tatcttcgga
    43441 cataagagct ccctgtcagc ggcagagccg taatgcagcc ctgccagagt ggatcaacgc
    43501 gctaaagctg cggtcagtgc cggaaggatc ttcacggcat cgccaacaat gccgtaatcc
    43561 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgacgcg
    43621 ttagcgccaa ccatgtgctg gatctacccg gagatcccca ccgccaggta cagttcaggt
    43681 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttct
    43741 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    43801 gcaatgttct ctttgctgcc aatgccgcga ccgacgctga ccaccagacg ggctttgtcg
    43861 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    43921 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    43981 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    44041 gccagaccgc cgtaaaccat gtgtttcact gttgctttac cgtcctgtac gctgacggtg
    44101 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    44161 ccgcgacggg tgtttggcaa cagcaccaga ccgtcagcgc cgtgctggcg aatagtgtca
    44221 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    44281 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggca
    44341 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agagggggta
    44401 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    44461 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    44521 gctgacgctc gcgctgtttc ggcgcggcaa cctgttgttc agaccaggcg tccacggcgt
    44581 taaaaccaat atccgccgcc gaccatacct ggactggctt tttcgccgcg ccgagaatgg
    44641 ctttcatcga aggaatttgt ggggagttga tatcagtgga aacagcaaca accgcaggca
    44701 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    44761 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    44821 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcagat
    44881 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    44941 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agcacatctt
    45001 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggctgtc acctgcgcct
    45061 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttgagatcg tattggctta
    45121 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    45181 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    45241 aggaaacgtc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    45301 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    45361 gttaagttcc tcaccaatat tgaaaacatg gcgtagcaaa aagaaatttt taatattgtt
    45421 ttatagatca ccaatattga aagtcagatg ctattttttt acccacaaaa gaatataaca
    45481 aactgattaa taaccaaaat taacgaaaaa cgccctctgt tcactcgaaa caaaaatgtg
    45541 acaccaatca cagaatacag cttattgaat acccattatg agttagccat taacgcgtcc
    45601 acgaggttaa taataattat attaaatgtt aacaaaaata aaacaaacgg gaacgcaaaa
    45661 taaatattcg ttttcacagt ggaattaact catgaagaat gaaaagagaa aaacgggaat
    45721 agaaccgaag gttttctttc cgccattaat aatcgtcggc atactttgtt ggcttacagt
    45781 cagagatctg gatgcggcga atgtcgttat taatgctgta ttcagttacg tcaccaatgt
    45841 atggggatgg gcatttgaat ggtatatggt ggtgatgctt ttcggttggt tctggctggt
    45901 gtttggcccg tatgccaaaa agcgtttagg taacgaaccg ccagaattta gcaccgccag
    45961 ttggatcttt atgatgttcg cctcctgtac gtctgctgcc gtactgttct ggggatcgat
    46021 tgagatctac tactacatct ccaccccgcc gtttggctta gaaccgaact cgacaggagc
    46081 gaaagagttg gggctggctt acagcttgtt ccactgggga cctctgccgt gggccactta
    46141 cagcttcctt tcagtcgcct tcgcttactt cttctttgtc cgcaaaatgg aagtgattcg
    46201 ccccagctcg acactggtgc cgctggtagg tgaaaaacac gccaaagggt tgttcggtac
    46261 catcgtcgac aacttctatc tcgtcgcctt gatcttcgcg atgggtacca gtctgggcct
    46321 tgccacgccg ctggtgaccg agtgtatgca atggttgttt ggcattccgc ataccctgca
    46381 actggacgct atcatcatta cctgctggat tatcctcaac gccatttgcg tcgcttgcgg
    46441 tctgcaaaaa ggggtacgta tcgccagtga cgtgcgtagt tacctgagct tcctgatgct
    46501 gggttgggtg ttcatcgtca gcggtgccag cttcatcatg aactacttca ccgattcggt
    46561 ggggatgttg ctgatgtatc tgccgcgcat gttgttctat accgatccca tcgctaaagg
    46621 cggcttcccg cagggctgga ccgtgttcta ctgggcatgg tgggtgattt acgccatcca
    46681 gatgagtatc ttcctcgccc gcatctcccg tggtcgtact gtgcgtgaac tgtgcttcgg
    46741 catggtgatg gggctgacag catccacctg gatcctgtgg accgtgctcg gtagtaacac
    46801 tctgctgttg atggataaaa acatcatcaa cattccaaat ctgatcgaac agtacggtgt
    46861 ggcgcgcgcc atcatcgaaa cctgggccgc tctgccactc agcaccgcca ccatgtgggg
    46921 cttcttcatc ctctgcttta ttgccaccgt tacgctggtt aacgcctgct cttataccct
    46981 ggcgatgtct acttgccgcg aagtacgcga tggtgaagaa ccaccgctgc tggtgcgtat
    47041 cggttggtca attctggttg gcattatcgg tattgttctg ctggcgctcg gcggcctgaa
    47101 accgattcaa accgccatta tcgccggagg atgcccgctg ttcttcgtca acattatggt
    47161 gacgctctcc tttattaaag acgcgaaaca gaactggaaa gattaattaa cccccaaaat
    47221 atcaagaggt tgaaagatgg attttaattt aaatgatgag caggaactgt ttgtcgccgg
    47281 tatccgcgaa ctgatggcca gcgaaaactg ggaggcctat tttgccgagt gcgaccgtga
    47341 cagcgtctac ccggaacgtt ttgtcaaagc actggcggat atgggtatcg acagtctgct
    47401 gatccctgaa gagcacggtg gtctggacgc ggggtttgtt actctcgccg ccgtgtggat
    47461 ggagctggga cgtctggggg caccaaccta tgtactgtac cagctgccgg gcgggttcaa
    47521 taccttcctg cgcgaaggca cacaagagca gatcgacaag attatggctt tccgcggcac
    47581 cggtaagcag atgtggaact cagcgattac cgaaccgggc gcgggctccg acgtgggtag
    47641 cctgaaaacg acttataccc gtagaaatgg taagatttat cttaatggta gtaagtgttt
    47701 tattaccagt agcgcctaca ccccgtacat cgtagtgatg gcgcgcgacg gggcttctcc
    47761 ggacaaacct gtctacaccg aatggtttgt tgatatgagc aaaccgggca tcaaagtgac
    47821 caaacttgag aagctcggtc tgcgtatgga tagctgctgt gaaatcacct ttgacgatgt
    47881 ggaactggac gagaaagaca tgttcggtcg ggaaggtaac ggctttaacc gcgtcaaaga
    47941 agagttcgac catgaacgtt tcctggtagc cctgaccaac tacggtacgg cgatgtgcgc
    48001 ctttgaagat gcggcgcgct acgccaacca gcgcgtgcag tttggcgagg ctattggtcg
    48061 tttccagttg attcaggaaa aattcgccca catggcgatc aaattaaact ccatgaaaaa
    48121 catgctgtat gaagcagcgt ggaaagcaga caacggcacc atcacctctg gcgatgcagc
    48181 gatgtgcaaa tacttctgcg ccaatgcggc atttgaagtg gtggatagcg caatgcaggt
    48241 gctgggcggt gtcgggattg cgggcaacca ccgcatcagc cgcttctggc gtgacctgcg
    48301 tgtagaccgc gtttccggag gatctgacga aatgcagatc ctgacgctgg gtcgtgcggt
    48361 gctgaagcaa taccgctaag tccgtgcgct gcctgatgcg acgctgacgc gtcttatcag
    48421 gcctacggga gcacgaatgt aagccggata aggcgtttac gccgcatccg gcagtcatgc
    48481 gctacataaa tttttcagga gctaataatg gatcatctac ccatgccgaa attcggaccg
    48541 ctggccggat tgcgcgttgt cttctccggt atcgaaatcg ccggaccgtt tgccggacaa
    48601 atgttcgcag aatggggcgc ggaagttatt tggatcgaga acgtcgcctg ggccgacacc
    48661 attcgcgttc aaccgaacta cccgcagctc tcccgccgca atttgcacgc gctgtcgtta
    48721 aatattttca aagatgaagg ccgcgaagcg tttctgaaat taatggaaac caccgatatc
    48781 ttcatcgaag ccagtaaagg tccggccttt gcccgtcgtg gcattaccga tgaagtactg
    48841 tggcagcaca acccgaaact ggttatcgct cacctgtccg gttttggtca gtacggcacc
    48901 gaggagtaca ccaatcttcc ggcctataac accatcgccc aggccttcag tggttacctg
    48961 attcagaacg gtgatgttga ccagccaatg cctgccttcc cgtataccgc cgattacttt
    49021 tctggcctga ccgccaccac ggcggcgctg gcagcactgc ataaagtgcg tgaaaccggt
    49081 aaaggcgaaa gtatcgacat cgccatgtat gaagtgatgc tgcgtatggg ccagtacttc
    49141 atgatggatt acttcaacgg cggcgaaatg tgcccgcgca tgagcaaagg taaagatccc
    49201 tactacgccg gttgcggtct gtataaatgt gccgacggct acatcgtgat ggaactggtg
    49261 ggcattaccc aaattgaaga gtgctttaaa gatattggcc tcgcacatct actcggtacg
    49321 ccggaaatcc cggaaggcac tcagcttatc caccgtatcg aatgccctta cggcccactg
    49381 gtagaagaaa aactcgatgc ctggctggcg gcacatacca tcgcagaagt taaagaacgc
    49441 tttgccgaac tgaatatcgc ctgtgccaaa gtactgaccg taccggaact ggaaagcaat
    49501 ccacagtatg ttgcccgtga atcaatcact cagtggcaaa cgatggatgg tcgcacctgc
    49561 aaagggccga acatcatgcc gaaattcaaa aataaccccg gacaaatctg gcgcggaatg
    49621 ccctcacatg gcatggacac ggctgccatt ttgaaaaata tcggctacag cgaaaacgac
    49681 attcaggagt tggtcagcaa aggtctggcc aaagttgagg actaaacgcg tggcgtcagt
    49741 cggcttgcct gactagcgcc cgcccaataa caaacgaaca aaaatggata gaggtgcaat
    49801 ggatatcatt ggcggacaac atctacgtca aatgtgggac gatctggcgg acgtttacgg
    49861 tcataaaacg gcgctgattt gtgaatccag cggcggagtc gttaaccggt atagttatct
    49921 tgagttaaat caggagatta accgcacggc aaacctgttt tatacgctgg ggattcgcaa
    49981 aggcaacaag gttgcactac atctcgacaa ctgcccggaa tttatctttt gctggttcgg
    50041 gctggcaaaa attggcgcga ttatggtgcc gattaacgcc cgcctgttac gcgaggaaag
    50101 cgagtggatc ttgcaaaata gccaggcgtg cctgctggtg accagtgcgc aattttatcc
    50161 tatgtatcaa cagattcagc aggaagatgc cactcaattg cggcacattt gcctgacaga
    50221 tgtggcactt cccgctgatg atggagtgag ttcatttact caactgaaaa atcaacaacc
    50281 tgccaccttg tgctatgcac cgccgctatc gactgacgat acggcggaaa ttctcttcac
    50341 ctccggcacc acctcccgac cgaaaggtgt ggtgattacc cattacaacc tgcgcttcgc
    50401 tggatattac tccgcctggc agtgtgcact gcgtgacgat gacgtctaca tgacggtaat
    50461 gcctgcgttt catatcgatt gccagtgtac tgcggcgatg gcggcgtttt ctgccggggc
    50521 cacctttgtg ctggtcgaga aatacagcgc ccgcgccttc tggggacagg cgcagaagta
    50581 ccgcgccacc attaccgaat gtattccgat gatgatccgt acgttgatgg tgcagccgcc
    50641 ttcagcgaac gatcggcaac accgcctgcg ggaagtgatg ttttatctca acttgtcgga
    50701 gcaggaaaaa gacgcatttt gtgaacgctt cggcgttcgc ttgctgacgt cttatgggat
    50761 gacggaaacc attgtgggca ttatcggcga tcgccctagc gataaacgac gctggccgtc
    50821 gattggtcgg gcggggtttt gctacgaagc ggagatccgc gacgatcaca atcgcccgct
    50881 cccggcaggt gagatcggtg aaatctgtat taaaggcgta ccagggaaaa ccatcttcaa
    50941 agagtatttt ctcaacccga aagccactgc gaaagtgctg gaagccgacg gctggctaca
    51001 taccggcgat accggatacc gcgacgaaga aggctttttt tatttcgtcg atcgccgctg
    51061 caatatgatc aaacgcggtg gcgagaatgt ctcctgcgtg gagctggaaa atatcatcgc
    51121 cacccatccg aaaattcagg acatcgtggt tgtgggtatt aaagattcga ttcgcgatga
    51181 agccatcaaa gcatttgtgg tgctgaatga aggtgaaaca ttgagcgaag aggaattttt
    51241 tcgcttctgc gaacaaaata tggcgaaatt taaagtgccc tcttatctgg agatcagaaa
    51301 agatctgcca cgtaattgct cggggaaaat aattagaaag aatctgaaat aaatgaaacg
    51361 tcagggaact accctgccag ctaataatca cgccctaaaa caatatgcat tttttgcagg
    51421 gatgttgtcg tccctaaaaa agcaaaaatg gagaaaagga atgagtgaat cattacatct
    51481 gacccgcaat ggatcaattc tggaaattac ccttgatcgc ccaaaagcga atgctattga
    51541 tgcaaaaacc agctttgaaa tgggcgaagt atttctaaat ttccgtgacg atccgcaatt
    51601 acgtgtcgcc attattaccg gtgccggaga gaaattcttt tccgcgggct gggatttaaa
    51661 agcggcagca gaaggcgaag caccggatgc tgactttggc ccgggtggtt ttgcgggatt
    51721 aaccgaaatt ttcaatctcg acaaaccagt tatcgcagct gtgaacggct acgcctttgg
    51781 cggcggtttc gaactggcgc tggcggcaga ttttattgtt tgtgccgata acgccagctt
    51841 cgccctgccg gaagccaaac tgggcatcgt tcctgacagc ggcggtgtgc tgcgtctgcc
    51901 gaagatcctg ccgcctacca tcgtcaatga aatggtgatg accggcagac gaatgggcgc
    51961 agaagaggcg ctgcgttggg ggatagtcaa ccgcgtggtt agccaggcgg aactgatgga
    52021 taacgcccgc gaactggctc agcagctggt taacagcgcc ccgctggcga ttgcggcgct
    52081 gaaagagatc taccgcacca ccagcgaaat gccggtagaa gaagcgtatc gctatattcg
    52141 cagcggcgtg ttgaaacact atccatcggt tctgcattcg gaagatgcca ttgaagggcc
    52201 gctggcgttt gccgagaagc gcgatccggt gtggaaagga cgttaacaac cgtgagctat
    52261 tacgcctttg aggggttaat tccggtggtt cacccgacgg cgtttgtcca tcccagtgcc
    52321 gtcttgattg gcgatgtgat tgtgggagcc ggtgtctaca tcggcccact cgcctcactg
    52381 cgtggtgact acgggcggtt gatcgtgcaa gcgggagcca atattcagga tggctgcatt
    52441 atgcatggct actgcgacac tgacaccatc gttggggaaa acggccatat cgggcatgga
    52501 gcgatcctgc atggttgtgt gattggtcgc gatgcattgg tcgggatgaa cagcgtaatt
    52561 atggatggcg cggtcattgg cgaagagagc attgttgccg caatgagctt tgtcaaagca
    52621 ggctttagtg gcgagaaacg ccagttgttg atgggtacgc cagcccgcgc tgtacgcagt
    52681 gttagtgacg acgagttaca ctggaaacgg ttgaatacca aagagtatca ggatcttgtc
    52741 gggcgctgtc atgcatcgtt acatgaaacg cagccactga ggcaaatgga ggaaaatcgc
    52801 ccccgtttgc aggggacgac ggatgtgacg cctaaacggt gatcttgccg gatgcgacct
    52861 ggttgctgga taagatgcta caacatcgta tctggcaagc aggacgcccg gtatcaacga
    52921 gataaaatta acgacgcata ctctttgaca acatcatctg ccacttctgc tcgcggttaa
    52981 gttccgtgag cggaacggca ggaatacgcg gtgtctttct tacgccgact aatttttccc
    53041 gactgttatt gcgcaggcgc gcgtaaattt gctcatcgat atcgaccact ttaaccagtc
    53101 gctgacactg gcatccccgc ccttccagct tattagggat catcttaact tcgcagctta
    53161 tttctgtgac ttccgacaga atataagtca gggtattaac cgccttgctg tgttcaatat
    53221 cgaaatgaat agagatctct cttgctatca cccaccgatt ttcagccatc atccattcgg
    53281 cgattaacaa gtagagtggt ttttcaacat atccttcaca catacgaact ccagtttcac
    53341 aaattaaaag tctggctatg actcgttttg aagcaattaa acaaggccat attaaaattg
    53401 tggatatatc gatagtatgc aacttcaccg tcgataagtg tgagctaaat cctgcttatg
    53461 tcatcaaaaa tatcgattcc ccaaaagatt tacttaatgg gcaaaaaaaa acggtcctca
    53521 tcagagaacc gtattagtta ttcgatcaaa ttagcggatg aaaaatatcc gccacgacac
    53581 gctattattt gatctgtgcg tgcatttcct gcaccgaaat tactttttca gtcgcatcgg
    53641 cattcagcgc catcgcggta gcgaaaccac cgttcagggt ggtgtcataa tgcactttat
    53701 attgcagcgc actgcgacgg atcacgcggg agtcttcaat cgcacgacgg cctgaggtgg
    53761 tgttgatgat gtaggtatat tcgccattct tgatacggtc ctgaatgtgc ggacggcctt
    53821 catgcacctt gtttaccaga cgcggattga tacccgcttc gcccagcaca atcgccgtgc
    53881 cgtgggtcgc atccagctcg aagccctgtt tcagcagttt tgccgccagg tccaccacgc
    53941 gttctttatc gccttcgcgc acggaaagca gcgcacgacc gtgtttcttc atggtggagt
    54001 tgctgcccag ctgcgctttg gcgaacgctt cagcgaaggt gcggcccacg cccatgactt
    54061 ccccggtaga gcgcatttct ggccctaaca gcgggtcaac gcctgggaat ttattgaacg
    54121 gcagcaccac ttctttcacc gagtagtacg gcggaataac ttctttggtt acgccctgct
    54181 cagccagcga tttgccagcc atcacacgcg ccgccacttt tgccagcggt acgccggtgg
    54241 ctttggagac gaacggaaca gtacgcgccg cacgcgggtt tacttcaatc aggtaaactt
    54301 cgttgttttt caccgcaaac tgcacgttca tcagaccgcg cacctgcaat tcgaaggcca
    54361 gtttctgcac ctgctggcgc atcacatcct gaatttcctg acttaaggtg tacgctggca
    54421 gcgaacatgc ggagtcaccg gagtgcacac ccgcctgctc gatgtgctcc atgatgccgc
    54481 caatcagcac catttcgccg tcgcagatgg catccacgtc aacttctact gcgtcatcaa
    54541 ggaaatgatc cagcaacact ggcgcatcgt tagacacgct aaccgccgtc tggaagtagc
    54601 gacgcaggtc agcttcgtca tagacgattt ccatcgcccg accgccgaga acgtaagacg
    54661 gacgcaccac cagcgggtag ccaatctctt ttgccttctc aactgccatt tcaatagcag
    54721 taacggtggc gttcgccggt tgtttcagtt tcagacggtc aaccgcatgc tggaagcgtt
    54781 cacggtcttc cgcacggtcg atagcatccg ggctggtgcc gataaccggt acgccagcgg
    54841 cttccagtgc acgcgccagt ttcagcgggg tctgaccgcc gtactgaacg ataacgcctt
    54901 tcggcttctc aatacgcacg atttccagca catcttccag agttaccggc tcgaagtaga
    54961 ggcgatcgga agtgtcgtag tcggtagaga cggtttccgg gttacagtta accataatgg
    55021 tttcgtaacc gtcttcacgc agcgccagcg aggcgtgtac gcagcagtag tcgaattcga
    55081 taccctgacc gatacggttt ggaccgccgc caagcaccat gattttttca cggtcggtag
    55141 acggattcgc ttcgcactct tcttcataag tggagtacat gtaagcggtg tcggtggcaa
    55201 actctgccgc acaggtatcc acgcgcttgt agaccgggtg caggtcatat tggtcacgca
    55261 gcttacggat ttccgcttcg cgtacgcccg ccagttttgc caagcgcgca tcggcaaagc
    55321 ctttgcgttt cagctggcgc aggaattcag cgttcaggcc agtgatgccc acttccgcca
    55381 ctttctcttc cagacgcacc agttcttcaa tctgtaccag gaaccagcgg tcaatgttag
    55441 tcaggttgaa gacgccgtcc acagacaggc ccgcgcggaa agcatcggcg atgtaccaga
    55501 tacgctctgc gcctgcgtct ttcagttcgc gacggatttt ggttaacgct tccgggtcat
    55561 ccaggctcac tttcgggtcg aagccagttg cgcccacttc caggccgcgc agcgcttttt
    55621 gcagggattc ctgctgcgtg cgaccaatcg ccatcacttc gccaaccgat ttcatctgag
    55681 tggtcagacg gtcgttagca ccggcgaatt tttcgaagtt gaagcgagga attttagtaa
    55741 ccacatagtc gatggacggc tcgaaggagg ccggagtacg tccgccagtg atgtcgttca
    55801 tcagttcgtc tagggtgtaa cccaccgcca gtttcgccgc cactttagca atcgggaaac
    55861 cggtcgcttt cgacgccagc gccgaagaac gggacacgcg tgggttcatt tcgataacaa
    55921 tcaggcgacc gtttttcggg ttcaccgcaa actggacatt ggaaccaccg gtttcaacgc
    55981 cgatttcacg cagcaccgcc atcgaggcgt tacgcatgat ttgatattct ttgtcggtca
    56041 gcgtttgggc tggcgcgaca gtgatggagt caccggtgtg gatgcccatc gcatcaaagt
    56101 tttcgataga gcagacgatg atgcagttgt cgtttttatc acgcaccact tccatctcgt
    56161 actctttcca gccgatcagc gactcatcaa tcagcaactc tttggtcgga gagagatcca
    56221 gaccgcgggc gcaaatttct tcaaactctt cacggttata agcgataccg ccgccgctac
    56281 cgcccatggt aaaggatggg cgaataatgc acgggaagcc cacgtcagcg gcaacagcca
    56341 gcgcttcttc catcgtgtgt gcgataccgg aacgcgcggt ttccagacca attttcttca
    56401 tcgctacgtc gaaacgacgg cggtcttctg ctttatcaat cgcatcggca gtggcaccaa
    56461 tcatggtgac accgaactct tccaacacgc cctgacgttc cagctccagc gcgcagttca
    56521 gcgccgtctg accgcccatg gttggcagca ccgcgtccgg gcgctctttt tcaataatct
    56581 tgcgtaccac ttcccagtga atcggctcga tgtaggtcgc atcggccatt tccgggtcgg
    56641 tcatgatggt cgccgggttg gagttcacca gaatgacgcg gtaaccctct tcgcgcaggg
    56701 ctttacatgc ttgcgcgcca gagtagtcaa actcacacgc ctgaccgata acaatcgggc
    56761 ccgcacccag aatcaggata ctttttatat ctgtacgttt tggcatggct cttttactcc
    56821 tgattactta gcggttttac ggtactgctc aattaacttg ataaagtggt cgaacaacgg
    56881 cgcggcgtcg tgtggaccag ggctggcttc agggtgaccc tggaagctga acgccggctt
    56941 atcgatgcga tgaatgccct gtaacgtacc gtcgaacagg gatttatgcg tgacacgcag
    57001 gtttgcaggt aatgttgctt catccaccgc aaaaccgtgg ttctgggcgg tgatcatcac
    57061 cacgtttttc tccacatctt taaccggatg gttgccgccg tggtgaccaa atttcatttt
    57121 gacagtcttc gcaccgctcg ccagcgccag cagctgatga ccgagacaga tgccgaatac
    57181 cggaatatcg gtttcgagga acttctggat ggcggtaatg gcgtaatcgc acggggccgg
    57241 gtcgccagga ccgttggaga ggaagatgcc gtctggattc attttcagca catcttccgc
    57301 agaagtttgc gccggaacga tggtcaggcg acagcctcta tccaccagca tccgcaggat
    57361 gttgcgcttg gcaccaaaat cataagccac gacgtggaac ggcagctcgt cttctttttt
    57421 cgcttctggc aggccgccgg tcaacgtcca gctcccttgt gtccagctat aggcttctgc
    57481 ggtggtcact tcttttgcca gatccatgcc gttcagacct gggaacgcgc gggctttttc
    57541 taacgccagc gccgcatccg ggttatcgcc tgcgataatg cagccattct gtgcgccttt
    57601 ctcgcgcagt aaacgcgtca gcttacgggt atcgatatcg gcaatcgcca cgatgttatg
    57661 gcgcttcagg taagaagaga ggtcttcggt attacggaag ttgctggcaa tcagcggcag
    57721 gtcgcgaatc accagacctt gtgcatgtac ctgagaagat tcttcatcgg cgtcattagt
    57781 gccgacattg ccaatatggg gataagtaag agtaacgatt tgacgagaat aggaaggatc
    57841 agtgaggatt tcttgataac cggtcattga agtattgaaa acgacttccc caaccgccga
    57901 acctgttgcc cctatggccc gaccgtgaaa ctgggttccg tcttccagaa ccaatagcgc
    57961 tgacttaatc aaaacaccct ccagagaata ctcactcact ttatttgcat attaattcac
    58021 agtgatgacg taaatcaatg caaatctgct taccgacgaa tttctggcaa acggcggcat
    58081 tctggagata tgggcgctaa aagtcaactt aatggcagga ttttttatat tattttgtgg
    58141 ctcatgcatg ttttacaggc attaagcgac aaaaacatca tctaacctgt tctggaaaac
    58201 gcttgcgcag caagaagatt gcatgaaata aaaacgagca ggaaaaagtg gaccaaatgg
    58261 tcaaaattta cattaaaaca acaaaaatag aagacgaaac atgtaatttt tggcagttat
    58321 agaaaattta aaagcataac accaaaaata aaagggctaa aaattaaagc cctttataat
    58381 taataaatta aatgtgttac ttttgcatca taataaacat tttgtggtta caaattattg
    58441 agatcaagca catctcgcat atcaaaaaga ccactttcct taccactcaa ccacaaagcc
    58501 gatcttaccg caccgttagc aaatgtcatg cggctggacg ccttatgggt gatctccaga
    58561 cgctcgccaa tatcggcaaa catcgcggta tgttcgccaa cgatgtcacc tgcacgcacg
    58621 gtagcaaaac caatggtacc tggcacacgt tcaccggtat ggccttcacg actgtagacc
    58681 gcgcaatctt tcagatcttt atcaagggcg tgggcgatcg cctctcccat tgccagtgcg
    58741 gtgcctgacg gcgcatcaac tttatgtcta tgatgtgctt caataatttc gatatcggtg
    58801 tagtcaccca tcactttggc tgctttctcc agcagcttaa gcatgacgtt aacgccaacg
    58861 ctaaaattgg cggcaaagac aatcgcaata tcggcggcgg cgtcacgaat tgcctgttta
    58921 ccggcttcgt caaaccccgt agtgccgatc accatccctt tgccatgctg ccgacaaaaa
    58981 gcgagatggt tcagcgtacc ttccggacgg gtaaaatcga taaacacatc aaaatcatct
    59041 tttatcgcat caagactgct ttgcacggta acgcctgttt tccctgcacc agccagctca
    59101 ccggcgtcgc tgcccagtaa agaagatcct tcgcgctcca gcgccgcgcc cagctgcacg
    59161 ccttctaatg ccagtgccgc ctgaatcaac tggcggccca tacgcccccc ggctcccgcg
    59221 atggcaacgc ggatgtttgc atcatgcata gctattctct tttgttaatt tgcatagacc
    59281 gttttcagag taaccagcgc atcgctacgc cgccagccaa aaacgctgat aattagaaat
    59341 taatgataaa cagggaggga tatcagtgaa aggcatgagt cgatgaatga ctgcatgccg
    59401 ataacatgtg acaggttacg gcaccagagc cagcacctca gccacccact gttggaagcc
    59461 ttttacattc agatccaatg ccacctgtac attggctggt ttgcccaggc aaccgtcgat
    59521 atcaaccacc gtcgtgcctg aggtaaattc gccttgagtt tctaccgcga caaaacacgg
    59581 cttgagggtg aataagtccg ggcgcaccag ccaggcgatg gcgcagagat cgtgcattcg
    59641 cagaccgctt tgcatactgc cgctacggta gtggctaaac agggcgtgga gcatttttcc
    59701 ggtgcgattc aactccggta aagtagcgag atagtcaggg gttaatattg cctgattggt
    59761 gacatccaga ccgcacatga caatttcgat gccgctacgg aagacacagg cagccgcttc
    59821 tggatcggca gcgatattaa actcggcatt tggtgtacag ttgccgcgac cagcagaacc
    59881 gcccatgatc accagacgac gaatatacgg tttacattcc gggcattgta aaagtaacag
    59941 cgcaatattg gttaacgggc cgatggccac cagggtaacg ggctctggtg cacgcatcag
    60001 ggcatcccga atcgccagaa acgccggtat cccgagtggc tttcggttgt gctcaacaaa
    60061 gtcgtagcca gccattcccg attcgccgtg cacagatgct gcatcacgcg gtgcgcgtac
    60121 cagcggcaca gcggctccct gggcgagcgg aatctccgca ttccagaaat gcagcagttg
    60181 cagggcattg cgggtagttt tctcaaccga gacattaccc gcgacggtgg tcatcagttg
    60241 caggtcgagt tcgggggcaa aaatcgcggc ggcaatggcg acggcatcgt caatgccggg
    60301 gtcagtatcg aggaagatag gtaaacgcat gttttcttca taaaaaatgc cggtaacaag
    60361 accggcattt tcgcataact taggctgcta atgacttaat cgacttcacg aatatcgaca
    60421 cgcagctctt tcggcacttc gaaaacaata ttttcttcac ggccttccag cggaatggct
    60481 tcaccaccac ccaactgctg caaacgtgcc accacattct gcaccagaat atccggagcc
    60541 gatgcgcccg cagtcacgcc gacgcattta acctctttca cccactcttc ctggatatct
    60601 ttcgcatcgt caatcaaaaa cgcgtgtttg cccatacgct gggccagctc cgccagacgg
    60661 ttggagttgg aggagttttt cgaaccgacc accaacacaa cttccgcctg ttctgccagg
    60721 gcgcgtaccg cttcctgacg gttagtcgtg gcgtagcaga tgtcatcttt gcgcggaccg
    60781 acaattttcg ggaagcgttt acgcagcgcg tcgatcacat cagacgtgtc atccaccgac
    60841 agcgtggttt gggtcataaa ggagagcttc tcttcgtttt tgaccgtcag tttccacaca
    60901 tcgtctggcg attcgaccag atacattccc ccttcggggt tgctgtactg ccccatcgtc
    60961 ccttccactt ccgggtgccc ggcgtggccg atgagaatag attcttcgcc acggcgactg
    61021 gcgcgggcaa cttccatatg cactttggtc accagcggac aggtggcatc aaacaccgtc
    61081 aaatcgcggc ttttcgcttc gttacgaacc gcctgagaaa caccgtgtgc ggagaaaatc
    61141 aggatcgcgc cgtccggcac ttcgctaatc tgctcaataa agatagcccc acgctcgcgc
    61201 aagctatcga ccacgtagcg gttatgcacc acttcgtgac ggacatatat cggtgcgccg
    61261 taaatggcca gcgcgttttc aacaatgctg atagcgcggt ctaccccggc acaaaaacca
    61321 cgcgggttgg ccaacaggat ctgcatgtta cgcctccagt gccggatcga tttccagcac
    61381 ttcaatatca aaatgaacgg tctgcccggc cagcggatgg ttgaaatcaa cggtaatgga
    61441 gtcgccgtta atttcgcgga tcacaccagg catctcactg ccatccattg cggtaaaaag
    61501 catgattgcg ccaatttctg gctcgcctgc atccataaat tcacggcggg agaagtactg
    61561 aatcaggtcc ggtgacggca cgccaaacgc cgcatctggc tccagcgaga aggtggtttt
    61621 gtcgcccact ttcagcccca gcaggtgttg ctccagccct tcagaaagag aagcatcgcc
    61681 caggcggaac agcgccggtt taccgttgtt gcgagtagac tcggcggtgg tgccatcgtc
    61741 gagttttagc gtgaagtgca ccaggacggc gctattgctc tgtacagatt cagacatgca
    61801 ggtttgctct tatttttgag gatttttgcc ggatgcggcc tacgatttcg tagcaatctg
    61861 taggccgcat ccggcagggt ttattattgt tttttcgctt tggaaggtaa aaacccttcc
    61921 agcacgatca gtgccgcacc gacacaaatg gcagtatcgg caaggttgaa ggtggcgaag
    61981 tgccagtcgc cgacgtagaa gtcgatcata tcgacaacga agccgtgcca caggcggtcg
    62041 aacaggttgc ccagcgcgcc gccaataatc agcgcgtaag cgatattgtt tagcttctgc
    62101 gtggccttcg agcgatacat catcactgcc aggagcacgc taataccaat cgcaataccg
    62161 gcaaagaacc aacgctgcca gccgccgcta tcggcaagga aactaaacgc cgcgccatag
    62221 ttacgcgcat aatgcagatt aagcgacggg aacagcggga ccgtatcccc cagagcaaag
    62281 ttctggagga tcaggtattt gctgcccaga tcgataatca gcacgactac caccagccac
    62341 agccagcgta gccctgttga acagatcgat tgactcatca ggcaaactta cgtttttcac
    62401 cgtcaccggc gacgttgctg acacagcggc cgcagatttc tgcgtgttcc gccaccttgc
    62461 cgacatcctg ggtgtagtgc cagcagcgtg ggcacttctc accttcggct ttactcaacg
    62521 cgactttcag ccctttgagt acttcgctct gctgagcatc agcaggtgcg tcgttatagt
    62581 ctgcaacggt agcgccggag gtcaacagga caaatcgtaa ttcatcgccc agcgcggtca
    62641 gtttcgccgc cagttccggt tctgcataca aggttactgc cgcttccagc gagccaccca
    62701 ctttcttgtc ggcacgcgct tgctcaatga ctttgttcac ttcgccacgc actttcaaca
    62761 gctcgtccca gaacgcatcg ttcattgctt cactgtctgc cagaccaaac aggccttcgt
    62821 accactcacc ggtgaagacg tatttttcac gttcgcccgg cagatagccc cacacttcat
    62881 cagcggtgaa ggagaggatt ggtgccatcc agcgcaccag cgcttcagcg atgtgataca
    62941 gcgcagtctg gcagctacga cgcgccacgc tatccgcttt ggcggtgtac tgacggtctt
    63001 tgatgatgtc gaggtagaag gaacccatct caacggagca gaagcgcatc agacgctgta
    63061 ccacttcgtg gaaatcgtat gcttcgtacg ccttgaggat gtcttcctgt gccgctttcg
    63121 cacaacctac ggcccagcga tccagtacca ccatctcttc cggtttcacc atatcttttg
    63181 ctggatcaaa accattcagg tttgccagca ggaagcgcgc ggtgttacgg atacgacgat
    63241 agctatcggc agcacgtttc aggatctcgt cagaaacggc catttcaccg gtgtagtcgg
    63301 ttgatgccac ccacagacgc agaatatccg cgcccagttt gttcatcaca tcctgcggcg
    63361 aaacggtgtt gccgatggat ttagacatct tgcgaccctg accatccacg gtaaagccgt
    63421 gggtcagtac ctgacgatac ggcgctttgc ctttcatcgc ggtggagatc attagggaag
    63481 acatgaacca accacggtgc tggtcagaac cttccagata catgtccgct gcgtgaccgg
    63541 caaattccgg acgcacgtca acaacagaag agtgggtaga tccggagtca aaccatacgt
    63601 cgagagtatc cggcactttc acatactggt cagcttcgtc gccgaggatc tctttcgcat
    63661 cgagatccca ccacgcctgg atgccgtcaa cttcaacgcg tttagccact tcttccatca
    63721 gttcgagggt acgcggatgc agctcttccg tgtctttgtg cacgaacagt gacatcggta
    63781 cgccccaggt gcgctgacgg gagatacacc agtcaggacg gttagcaacc atcgactcga
    63841 tacgcgcctg gccccagtcc gggagccact gcacgccttt gatctctttc agtgactgcg
    63901 cacgcagacc tttctgatcc atgctgacga accactgcgg tgtcgcgcgg aagatgatcg
    63961 gcgttttgtg acgccagcag cacggatagc tgtgctgcat tttctcaacg tgcagcagcg
    64021 cgcctttttc ctgcagcagc gcaacgacga tgtcgttcgc tttgaagacg ttcacgccat
    64081 ccagcgtcgg ataagtgccc ggcagataag tgccgtccgg gccaaccggg ttagcggttt
    64141 ccaggccgta tttctgaccg atcacatagt cgtccgggcc gtggccaggc gcggtgtgaa
    64201 cggcaccggt accggcatcc agggtaacgt gatcgccgag gattgccgga acgtcgaagc
    64261 ccataaacgg atgggtaaag cgcagcagct caagctctgc accttttacc gtgccgagaa
    64321 tggtgtaatc ggtcacgccg atacgctgca ttacgctttc aaccagatct ttcgccagaa
    64381 tcacggcctg accgtcgatc tgcaccagcg catagtcgaa atcaggtgca atagagattg
    64441 cgcggttcgc aggcagagtc cacggcgtag tggtccagat caccagcgag attgggccgt
    64501 taacgttgct tacgccaaat ttcgctttca gcgcatcctg atcgaccgcc tggaaagcga
    64561 cgtcaatgga cggagaagtt ttgtcgtaat actcaacttc cgcttctgcc agtgcagaac
    64621 ggcagtctac gcaccagtgc accggcttcg cgcctttgtg caggtgacca ttgccgatga
    64681 ttttgcccag cgcgcggatg atgttggctt cagttttgaa gtccatagtc agatacgggt
    64741 gcgaccagtc gcccagcacg cccagacgga taaagtcttt gcgttgaccg tcaacctggg
    64801 tcgccgcgta ttcgcggcac ttggcgcgga actcggcggc ggtgaatttc tcacccggct
    64861 taccgtattc ttgctctact ttcagctcga tcggcagacc gtggcagtcc cagccaggca
    64921 catacggcga gtcataaccg gaaagccctt tggacttcac gataatgtct ttcagaatct
    64981 tgttaaccga gtgaccaata tgaatgctgc cattcgcata aggagggcca tcatgcagaa
    65041 tgaaggtttt tttgcctttt ttagccgcac ggatgatgcc gtacagatca tcatcagtcc
    65101 aacgcgccag cattcccggt tcgcgcttgg cgagatcgcc acgcatcggg aaccctgttt
    65161 ccggcaaatt cagggttgat ttatagtcac tcatcagatt ctcggttccg tgtttcggtt
    65221 tgattacata acaggcttaa gccggttttg ttagcccaaa aaattcgcgg gcggttaatt
    65281 catcacgcgc aatctgcgct ttcagttcgt ccagcgacgc aaatcgctgc tcattgcgta
    65341 tttttttacg cagcactact tgtatatggc gaccgtaaag gtccattgca acatctaaca
    65401 aatgcacttc cagctgctgg cgaataccgg caaccgttgg gcgtgttccg atgtttgcca
    65461 cgccgggtaa cggcttttca ccgaggccca gcacttctac cgcataaacc cctttcaccg
    65521 gggaaacctg acggcgtagc ggtacattcg ccgtcgggaa acctatagtg cgccctaatt
    65581 catcaccgtg gactacacgc ccggagatgg caaacgggtg ccccagtaaa ctttctgcca
    65641 gagccagatt gtcatcagcc agcgcctgac gcacggcggt gctgctgata cgcacaccac
    65701 cttcgcaaaa agtttgcgta ctggtgatat cgaagccgta ttccatgcca gctttctgta
    65761 ataacaagaa atcgccttca cgaccagcgc caaagcggaa atcatcacct acggcaagaa
    65821 attttacgcg caaatgcttc accaaaagat cgctgatgaa attttgcgcg gttaacgccg
    65881 caaaacgcct gtcgaaacgc acgcacagca cgtaatcaac gccacactct gcaaggtaac
    65941 gcagtttttc ccgcagccgg gtcagtcttg ccggggcttt atcggtagca aacagttcca
    66001 gtggttgagg ttcaaaaagc atcaccatca ccggtaagtt gcgcttgcgc ccttcttcct
    66061 gcaagccctg taacagcgcg cgatgaccgc gatgcacgcc gtcgaaatta ccaatagtca
    66121 gcacacaccc ttcttgcggg gcctggctga gattatgtat gccgcgtatc agcttcatgt
    66181 ctggctcaaa acagtgaaaa tcgtccgagt ataccttgta cagcggtcaa ggttaaccgg
    66241 cgattgagta ccgaaatcag aaagaagtgg cttttcattg ccatggagca aatcacggga
    66301 agaaactgac cgcctgctgc aatttttatc gcggaaaagc tgtattcaca ccccgcaagc
    66361 tggtagaatc ctgcgccatc actacgtaac gagtgccggc acattaacgg cgcttatttg
    66421 cacaaatcca ttgacaaaag aaggctaaaa gggcatattc ctcggccttt gaattgtcca
    66481 tatagaacac atttgggagt tggaccttgg ctaatatcaa atcagctaag aagcgcgcca
    66541 ttcagtctga aaaggctcgt aagcacaacg caagccgtcg ctctatgatg cgtactttca
    66601 tcaagaaagt atacgcagct atcgaagctg gcgacaaagc tgctgcacag aaagcattta
    66661 acgaaatgca accgatcgtg gaccgtcagg ctgctaaagg tctgatccac aaaaacaaag
    66721 ctgcacgtca taaggctaac ctgactgcac agatcaacaa actggcttaa tcgccaattt
    66781 gctgaagctt tgtgaaaaag cccgcgcaag cgggtttttt tatgcctgct gcttttgtga
    66841 tgcgtgagag aacacactgc cggttgccag atagtcaaaa gagacatgcc gttgtactat
    66901 ctgaactctt cgtggcttaa gaatcgtaga accatcggct gatacattct gaacaagaat
    66961 atctactcaa tttccagact cccccacaaa gaatatggat attgtgatac acattgaggt
    67021 attaaactta agaccactct atttttgaat taaattccta tatactttaa ccattcataa
    67081 atatctcctc attagacctt
//
LOCUS       DAEEGA010000009        66140 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEEGA010000009 DAEEGA010000000
VERSION     DAEEGA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66140
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.009"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..88
                     /locus_tag="KLH46_000595"
     CDS             <1..88
                     /locus_tag="KLH46_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0377408.1"
                     /translation="VRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(236..715)
                     /gene="folK"
                     /locus_tag="KLH46_000596"
     CDS             complement(236..715)
                     /gene="folK"
                     /locus_tag="KLH46_000596"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBE0377409.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(712..2130)
                     /gene="pcnB"
                     /locus_tag="KLH46_000597"
     CDS             complement(712..2130)
                     /gene="pcnB"
                     /locus_tag="KLH46_000597"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBE0377410.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2169..3095)
                     /gene="gluQRS"
                     /locus_tag="KLH46_000598"
     CDS             complement(2169..3095)
                     /gene="gluQRS"
                     /locus_tag="KLH46_000598"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBE0377411.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3132..3587)
                     /gene="dksA"
                     /locus_tag="KLH46_000599"
     CDS             complement(3132..3587)
                     /gene="dksA"
                     /locus_tag="KLH46_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBE0377412.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3765..4469)
                     /gene="sfsA"
                     /locus_tag="KLH46_000600"
     CDS             complement(3765..4469)
                     /gene="sfsA"
                     /locus_tag="KLH46_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBE0377413.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4484..5014)
                     /gene="thpR"
                     /locus_tag="KLH46_000601"
     CDS             complement(4484..5014)
                     /gene="thpR"
                     /locus_tag="KLH46_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBE0377414.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            5088..7517
                     /gene="hrpB"
                     /locus_tag="KLH46_000602"
     CDS             5088..7517
                     /gene="hrpB"
                     /locus_tag="KLH46_000602"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBE0377415.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATQD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7713..10247
                     /gene="mrcB"
                     /locus_tag="KLH46_000603"
     CDS             7713..10247
                     /gene="mrcB"
                     /locus_tag="KLH46_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBE0377416.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10467..12710
                     /gene="fhuA"
                     /locus_tag="KLH46_000604"
     CDS             10467..12710
                     /gene="fhuA"
                     /locus_tag="KLH46_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBE0377417.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12761..13558
                     /gene="fhuC"
                     /locus_tag="KLH46_000605"
     CDS             12761..13558
                     /gene="fhuC"
                     /locus_tag="KLH46_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBE0377418.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13558..14448
                     /gene="fhuD"
                     /locus_tag="KLH46_000606"
     CDS             13558..14448
                     /gene="fhuD"
                     /locus_tag="KLH46_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBE0377419.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14445..16427
                     /gene="fhuB"
                     /locus_tag="KLH46_000607"
     CDS             14445..16427
                     /gene="fhuB"
                     /locus_tag="KLH46_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBE0377420.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16462..17742)
                     /gene="hemL"
                     /locus_tag="KLH46_000608"
     CDS             complement(16462..17742)
                     /gene="hemL"
                     /locus_tag="KLH46_000608"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBE0377421.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17967..19388
                     /gene="clcA"
                     /locus_tag="KLH46_000609"
     CDS             17967..19388
                     /gene="clcA"
                     /locus_tag="KLH46_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBE0377422.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19412..19477
                     /locus_tag="KLH46_000610"
     CDS             19412..19477
                     /locus_tag="KLH46_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377423.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19470..19814
                     /gene="erpA"
                     /locus_tag="KLH46_000611"
     CDS             19470..19814
                     /gene="erpA"
                     /locus_tag="KLH46_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBE0377424.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19861..20484)
                     /locus_tag="KLH46_000612"
     CDS             complement(19861..20484)
                     /locus_tag="KLH46_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBE0377425.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20522..21322)
                     /gene="btuF"
                     /locus_tag="KLH46_000613"
     CDS             complement(20522..21322)
                     /gene="btuF"
                     /locus_tag="KLH46_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBE0377426.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21315..22013)
                     /gene="mtnN"
                     /locus_tag="KLH46_000614"
     CDS             complement(21315..22013)
                     /gene="mtnN"
                     /locus_tag="KLH46_000614"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBE0377427.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            22097..23614
                     /gene="dgt"
                     /locus_tag="KLH46_000615"
     CDS             22097..23614
                     /gene="dgt"
                     /locus_tag="KLH46_000615"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBE0377428.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23744..25168
                     /gene="degP"
                     /locus_tag="KLH46_000616"
     CDS             23744..25168
                     /gene="degP"
                     /locus_tag="KLH46_000616"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBE0377429.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25323..26479
                     /gene="cdaR"
                     /locus_tag="KLH46_000617"
                     /pseudo
     CDS             25323..26479
                     /gene="cdaR"
                     /locus_tag="KLH46_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26568..26954)
                     /locus_tag="KLH46_000618"
     CDS             complement(26568..26954)
                     /locus_tag="KLH46_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBE0377430.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27268..28092)
                     /gene="dapD"
                     /locus_tag="KLH46_000619"
     CDS             complement(27268..28092)
                     /gene="dapD"
                     /locus_tag="KLH46_000619"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBE0377431.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28123..30795)
                     /gene="glnD"
                     /locus_tag="KLH46_000620"
     CDS             complement(28123..30795)
                     /gene="glnD"
                     /locus_tag="KLH46_000620"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBE0377432.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30857..31651)
                     /gene="map"
                     /locus_tag="KLH46_000621"
     CDS             complement(30857..31651)
                     /gene="map"
                     /locus_tag="KLH46_000621"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBE0377433.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32019..32744
                     /gene="rpsB"
                     /locus_tag="KLH46_000622"
     CDS             32019..32744
                     /gene="rpsB"
                     /locus_tag="KLH46_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBE0377434.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33002..33853
                     /gene="tsf"
                     /locus_tag="KLH46_000623"
     CDS             33002..33853
                     /gene="tsf"
                     /locus_tag="KLH46_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBE0377435.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34000..34725
                     /gene="pyrH"
                     /locus_tag="KLH46_000624"
     CDS             34000..34725
                     /gene="pyrH"
                     /locus_tag="KLH46_000624"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBE0377436.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDSTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35017..35574
                     /gene="frr"
                     /locus_tag="KLH46_000625"
     CDS             35017..35574
                     /gene="frr"
                     /locus_tag="KLH46_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBE0377437.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35666..36862
                     /gene="ispC"
                     /locus_tag="KLH46_000626"
     CDS             35666..36862
                     /gene="ispC"
                     /locus_tag="KLH46_000626"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBE0377438.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            37051..37809
                     /gene="ispU"
                     /locus_tag="KLH46_000627"
                     /gene_synonym="uppS"
     CDS             37051..37809
                     /gene="ispU"
                     /locus_tag="KLH46_000627"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBE0377439.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37822..38679
                     /gene="cdsA"
                     /locus_tag="KLH46_000628"
     CDS             37822..38679
                     /gene="cdsA"
                     /locus_tag="KLH46_000628"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBE0377440.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38691..40043
                     /gene="rseP"
                     /locus_tag="KLH46_000629"
     CDS             38691..40043
                     /gene="rseP"
                     /locus_tag="KLH46_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBE0377441.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40073..42505
                     /gene="bamA"
                     /locus_tag="KLH46_000630"
     CDS             40073..42505
                     /gene="bamA"
                     /locus_tag="KLH46_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBE0377442.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42627..43112
                     /gene="skp"
                     /locus_tag="KLH46_000631"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42627..43112
                     /gene="skp"
                     /locus_tag="KLH46_000631"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBE0377443.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43116..44141
                     /gene="lpxD"
                     /locus_tag="KLH46_000632"
     CDS             43116..44141
                     /gene="lpxD"
                     /locus_tag="KLH46_000632"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBE0377444.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44246..44701
                     /gene="fabZ"
                     /locus_tag="KLH46_000633"
     CDS             44246..44701
                     /gene="fabZ"
                     /locus_tag="KLH46_000633"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBE0377445.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44705..45493
                     /gene="lpxA"
                     /locus_tag="KLH46_000634"
     CDS             44705..45493
                     /gene="lpxA"
                     /locus_tag="KLH46_000634"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBE0377446.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45493..46641
                     /gene="lpxB"
                     /locus_tag="KLH46_000635"
     CDS             45493..46641
                     /gene="lpxB"
                     /locus_tag="KLH46_000635"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBE0377447.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46638..47234
                     /gene="rnhB"
                     /locus_tag="KLH46_000636"
     CDS             46638..47234
                     /gene="rnhB"
                     /locus_tag="KLH46_000636"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBE0377448.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47271..50753
                     /gene="dnaE"
                     /locus_tag="KLH46_000637"
     CDS             47271..50753
                     /gene="dnaE"
                     /locus_tag="KLH46_000637"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBE0377449.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50766..51725
                     /gene="accA"
                     /locus_tag="KLH46_000638"
     CDS             50766..51725
                     /gene="accA"
                     /locus_tag="KLH46_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBE0377450.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51824..53965
                     /gene="ldcC"
                     /locus_tag="KLH46_000639"
     CDS             51824..53965
                     /gene="ldcC"
                     /locus_tag="KLH46_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBE0377451.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYHNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54022..54411
                     /locus_tag="KLH46_000640"
     CDS             54022..54411
                     /locus_tag="KLH46_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE0377452.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54476..55774
                     /gene="tilS"
                     /locus_tag="KLH46_000641"
     CDS             54476..55774
                     /gene="tilS"
                     /locus_tag="KLH46_000641"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBE0377453.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55823..56083)
                     /gene="rof"
                     /locus_tag="KLH46_000642"
     CDS             complement(55823..56083)
                     /gene="rof"
                     /locus_tag="KLH46_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBE0377454.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56070..56270)
                     /locus_tag="KLH46_000643"
     CDS             complement(56070..56270)
                     /locus_tag="KLH46_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBE0377455.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56436..56981
                     /locus_tag="KLH46_000644"
     CDS             56436..56981
                     /locus_tag="KLH46_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBE0377456.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56978..57400
                     /gene="arfB"
                     /locus_tag="KLH46_000645"
     CDS             56978..57400
                     /gene="arfB"
                     /locus_tag="KLH46_000645"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBE0377457.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57414..58124
                     /gene="nlpE"
                     /locus_tag="KLH46_000646"
                     /gene_synonym="cutF"
     CDS             57414..58124
                     /gene="nlpE"
                     /locus_tag="KLH46_000646"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBE0377458.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58279..59103)
                     /locus_tag="KLH46_000647"
     CDS             complement(58279..59103)
                     /locus_tag="KLH46_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBE0377459.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59156..60874)
                     /gene="proS"
                     /locus_tag="KLH46_000648"
     CDS             complement(59156..60874)
                     /gene="proS"
                     /locus_tag="KLH46_000648"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBE0377460.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60985..61692)
                     /gene="tsaA"
                     /locus_tag="KLH46_000649"
     CDS             complement(60985..61692)
                     /gene="tsaA"
                     /locus_tag="KLH46_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBE0377461.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61689..62093)
                     /gene="rcsF"
                     /locus_tag="KLH46_000650"
     CDS             complement(61689..62093)
                     /gene="rcsF"
                     /locus_tag="KLH46_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBE0377462.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62211..63026)
                     /gene="metQ"
                     /locus_tag="KLH46_000651"
     CDS             complement(62211..63026)
                     /gene="metQ"
                     /locus_tag="KLH46_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBE0377463.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63066..63719)
                     /gene="metI"
                     /locus_tag="KLH46_000652"
     CDS             complement(63066..63719)
                     /gene="metI"
                     /locus_tag="KLH46_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBE0377464.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63712..64743)
                     /gene="metN"
                     /locus_tag="KLH46_000653"
     CDS             complement(63712..64743)
                     /gene="metN"
                     /locus_tag="KLH46_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBE0377465.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64931..65506
                     /gene="gmhB"
                     /locus_tag="KLH46_000654"
     CDS             64931..65506
                     /gene="gmhB"
                     /locus_tag="KLH46_000654"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBE0377466.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65870..>66140
                     /locus_tag="KLH46_000655"
     rRNA            65870..>66140
                     /locus_tag="KLH46_000655"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15657 a        16371 c        18229 g        15883 t
ORIGIN      
        1 ggtacggaag tcactgtcgt tctcaaaatc ggtggagctg catgacaagg tcatcgggca
       61 ttatctgaac ataaaacact atcagtaagt tggagtcatt accggcgatg ggattgatat
      121 ttatctgcgg gaatttatat tatgtttctg caaaaatgtt taaactaaaa tttttattaa
      181 ggaaagagag gatgtatgat tttcagaaaa tatttatatt cgtaatataa ataaattacc
      241 atttgcttaa tttgtcaaat gctcttgtat gtaagacttc acgcaacgtc tccccatcag
      301 gaaacgccag ctccggtgca atctcaaaca gcggccacag cataaatcca cgattcttca
      361 tatcgtagtg cggaacggtc aggcgttcgg tatttatcac ttcattacca aacagcatga
      421 tatcgagatc cagcgtgcgt ggtccccagc gttcagcttt acgaacacga ccctgttgca
      481 gttctatgcg ctgtgtatgg ttgagtaact cttccggggc gggcgaagtt tcaagcgcca
      541 cggcggcgtt gaggtaatcg ggttgatctt gcggccccag cggtggggtg cggtaaaacg
      601 aagaaacggc aagaatgcgg ctttcaggga tatcgcctaa tgctttcagg gcagcattga
      661 cctgctccag cggagaggcc agattgctgc ctatggcaat atacgccact gtcatgtggt
      721 accctcacga cgcggtgcgc gtttgcgtgg acgacgagta cgacgacgcg gtgacggttc
      781 ttcatccagc tcgttaagca tccctttttg atctggtggt gcggaaacct ggaactcacc
      841 ccaccatttc accagacgct gcagttcagc attacgttca acttcagctc gcaaggccaa
      901 caggtcataa gccgcacgga acttaggatg ctccagcagt ttccatgcgc gtttaccctg
      961 acgacgggac atacgcaact gcaactgcca gatatcgcgg gttaacgtcg tcagacgttt
     1021 cgggattgcc agtgaacggc aggcttcgtc cagcacgtcg ttcatcgcca gcgcgaaagc
     1081 gtcgtgatag gtcaggccgc tttcctgggc gatcttctgt gccgtctcca gcagtgggta
     1141 ccagaacatg gcggcaaaca agaacgccgg gttcacgcgc atatcgttat ggatacgcgt
     1201 atcggtattc ttcagcacct gttcaatgat ccgctccatc gggctgtcgc cattttccgt
     1261 gaagtagcgg gtaatggtcg ggaacagcgg ctggaacaga tgatattcac acaacagctt
     1321 ataggtttcg taaccgtagc ccgcttgtag cagtttaagc gattcttcaa acaggcgtgc
     1381 cggtgggata tcgttcagca gggtagcgag gcgagggatc ggttctgcgg tttccgggct
     1441 gatgcgcata cccaatttgg cggcaaaacg taccgcgcgc agcatacgta ccggatcttc
     1501 acggtagcgc gtttccgggt taccaatcag acggataacg ccgtccttca gatccttcat
     1561 accaccaacg taatcacgga cggtaaaatc cgctacgctg taatacaggc tgttgatagt
     1621 gaaatcgcgg cgctgggcgt cttcttcgat ggagccgaaa atgttgtcgc gcagcaacat
     1681 gccgttttgc ccgcgttggg aggtcgtgcg gtcgctgacg ttaccttcgt ggtgtccacg
     1741 gaaggtcgca acttcgataa tctccgggcc aaacattaca tgagccagac ggaaacggcg
     1801 acccaccagg cggcagttac ggaacagttt gcgcacctgc tcaggcgtgg cgttagtggt
     1861 tacgtcaaaa tctttcggct ttttgccaag taacaggtcg cgcacgccgc cgccaaccag
     1921 ccaggcttcg tatcccgctt tgttgagcct gtacattacc ttcagggcat tttcactgat
     1981 atctttgcgg gaaatagcat gctgctcacg cgggatcacc gtcacctgtg gacgggcgac
     2041 tgcctgttca gcctcgcttt cctcgcggct tagcaccttg cggcaaaaat tagcgactcg
     2101 ggtaaaaata gtacacctcg gtagtgtcaa acatcattca ggacaaaaaa atagcggcta
     2161 atcatagctc agcatgacgc atttgagaat gttgaattta caattgccga ctcgggcacg
     2221 gcggtcagca tccagttttt gacggctgac tgaaggattt gctcgacgct gaaatcctgc
     2281 cagtgtgctt ctgcctgctg ccccagaaat tgaagtgccg cgattagtac cgggcgtgga
     2341 tcgcctttcg gcaacgcagg cgcatgattc tgcttggaaa gtttagcgcc ttgtggatta
     2401 agcgccagcg gcagatgaat gtaatcaggc actttccagc caaaaagctg gtacagcggg
     2461 atttgcctta ctgttggttc aatcagatca gccccacgca ctatttctgt aacgccctgg
     2521 aaatgatcat caaccacaac agccaggttg taggcgaaca acccatcacg gcgatgaatg
     2581 ataaaatctt cccgtgccag tttttcgtcg gcgtgaataa tgcctcgcag caggtcagta
     2641 aattgcgtga ccggatgctg ctggcggata cgcactgcgg cgttgtctgg tccatgatgc
     2701 aacacccggc aatgaccgtc gtaaataccg ccaatgcttt gaatacgcgc acgcgtacag
     2761 gtgcagtaat aacttagtcc ttgttcatgt aaccaggcga gtgcttcacg ataggcgtcg
     2821 tgacgttgcg attgccagag aacatcgccg tcccagtgca gaccgtaatg ttccagctgg
     2881 cgcaggatag tttctgcggc accgggaact tcacgaggcg ggtcgatatc ttctatgcgt
     2941 accagccagc gaccttgccg ggcgcgagcc tgcaaatagc tgccgagcgc ggcgatcaga
     3001 gagccaaaat gaagctcgcc ggaaggagag ggggcgaagc ggccaatata ctgtgtgtct
     3061 gtcatctctt tgaacaaaaa ataaggcggg agcatttccc gcctgtggta aacgtgatgg
     3121 aacggctgta attagccagc catctgtttt tcgcgaattt cagccagcgt tttgcagtcg
     3181 atgcacagat cggctgtcgg gcgcgcttcc agacggcgaa taccaatttc aacaccgcag
     3241 gattcgcagt agccgaaatc ttcgtcttcc acttttttca gcgtcttctc gatctttttg
     3301 atcagcttac gctcgcgatc gcggttacgc agttcgaggc tgaactcttc ttcctgggct
     3361 gcacggtcta ccgggtccgg gaagttggct gcttcatcct gcatatgtgt aacggtgcga
     3421 tcgacttcat ccctgagttg attacgccat gcttccagaa tacgacggaa gtgcgccagc
     3481 tgggcttcat tcatatactc ttcgcccggc ttctcctgat atggttccac cccagcgatg
     3541 gcgagaatac tcagggacga tgttttacgg ttttgccctt cttgcatgtt gcttctcctt
     3601 aacacgcact atcgatcccc atgttcgggg gaaaaatgag gccgctataa atagcagatg
     3661 cttttccgga tagcaattat ctaaacgtaa cacttgacaa ctgtgtgagg aaaagcgtat
     3721 ttgcgcacgc gaccagaatg taaattaacc agttacttac tttactacaa tgtaaccggc
     3781 agtgattttt taagagccat gccttcagca gaaatttccg ctttgtaagc cagaatttct
     3841 acccccctct gttgagcttc tgacaatagt tgcgcgtatt tctcatcgat gtggcgcgcg
     3901 ggtgaaaacc gtgtaatggc tgaatgcagc acggcgaaaa aaataaccgc acgctggcct
     3961 tcagccgcta cgctcatcaa ctcccgaagg tgtttctgac ctcgttcagt gaccgcatcg
     4021 ggaaaatatc cctgttcgtt ctccgctaac gtaaccgatt tcacttcaat atagcagtct
     4081 ggacgcgaat ccgcctgcaa cataaagtca atacggctgc gttcggagcc gtattttact
     4141 tcgcttttca gcgagctata gcctgacagt tctgaaattg attcattaag gatagcctct
     4201 ttcgtcaacc tgttagccca aagcgtattg acgcaaataa atgcgccgct ctggctttga
     4261 gttaattccc aggtgtgtgg gtatttccgt ttggtgttgt ctgaagtcga ataccagacg
     4321 gtatcgccag gcgttgcaca accggtcatc gcacccgtat tcgggcagtg tagcgttaat
     4381 tcgcgaccat cgggtgtgat cacatcggct aaaaaacgtt tgtaacgctg aattagcgtc
     4441 gcgcgctgta gagggggaga aaattccatt gcgacaatcc ttgttattgc gttagcgccc
     4501 agcgttttag cggcgtgtag cgtgtgcgtc cacgggcaaa cgaggaggcg taaagggtga
     4561 actccgtcac cgcataagac cagttaaaac ccggcggcgg gaatgtcacc gcctcgctgg
     4621 cgtcgcgcaa taaggtaata tgtggatgaa acggacgatt gctttgaaaa caaccgctgc
     4681 gggcagcctg tgaacggagc atattcgcca gctggattaa gccgcgtgga ggctgacgca
     4741 tccctaacca caccacacgc gaacgcagcc attgtccggc gtcatcaagc gtgagtgtga
     4801 aaccaggttg acgaatccgt ccggctaaaa gagaaagcgc cttctctttc tctgcgctca
     4861 cttcgcctaa aaatgccaga gtcaggtgca aattatcggc ggcgaccgga cgtcccgcct
     4921 caggtgggaa gtgtgtggcg cgccaatgga taatctgttc gcggatttct gcaggtaagt
     4981 cgatagcaaa gaacagacgt tgcggttcag acatgtgagg cactcggtta tgaattaccg
     5041 cgatgctaca atgtggcgcg aagaatgtta accctctgga gcgttttgtg tcgtcgttgc
     5101 ccgttgctgc cgtcttacct gaattactta ccgctctcga ttgtgcgccg caggtattat
     5161 taagtgcgcc gaccggggcc gggaaatcaa cctggctgcc gctgcaactg ctggcgcatc
     5221 ccggcattaa cgggaaaatt atcctgctgg agccgcgtcg tctggcggcg cgtaacgtcg
     5281 cgcaacggct ggcggagctg cttaacgaaa agccaggcga taccgttggc taccggatgc
     5341 gtgcgcaaaa ctgcgtcggg ctgaataccc gcctggaagt ggttaccgaa ggcgtgctga
     5401 cgcggatgat ccagcgtgac ccggaactga gcggtgttgg attggtgatc cttgatgagt
     5461 ttcatgagcg cagtttgcag gcggatttgg cgttggcgct gttactcgat gtgcaacaag
     5521 gtctgcgtga tgaccttaaa ctgctgatta tgtcggctac gcaggacaat gaccgcttgc
     5581 agcaaatgct gccggaagcg cccgtcgtca tctcagaagg gcgctcgttt ccggttgaac
     5641 gccgttattt accgctgccc gcgcatcagc gttttgacga agccgttgcg gtagccaccg
     5701 ccgaaatggt gcgtcaggaa agtggatcat tactgttatt tttacctggc gtcggagaaa
     5761 ttcagcgcgt gcaggaacaa ctggcttcgc gcatcggcag tgatgtggtg ctctgcccgc
     5821 tgtatggcgt gttgtcactg aacgatcagc gaaaagcgat cctcccggca ccgcaaggga
     5881 tgcgcaaagt ggtgctggcg accaatattg ctgaaaccag tttaaccatc gaaggtattc
     5941 gtctggtggt ggattgtgcc caggagcgtg tggcgcgctt tgatccgcgc acggggctta
     6001 cgcggttgat tactcaacgc gttagtcagg catccatgac gcagcgtgcc gggcgcgccg
     6061 ggcgtctgga gccgggtatc agcctgcatt taatcgccaa agaacaagca gaacgcgccg
     6121 cggcgcaaag tgagccggag atcttacaaa gcgatctttc cggtttgctg atggaattac
     6181 tgcaatgggg atgcagcgat ccggcgcaga tgagctggct ggatcaaccg ccaacggtga
     6241 atctactggc cgcgaaacgc ctgttacaaa tgctgggggc gctggagggt gaacggctta
     6301 gtgcgcaagg gcaaaaaatg gcagcgctgg gtaacgatcc gcgtttagcg gcaatgctgg
     6361 ttaacgcaaa gagcgatgac gaagctgcta ccgcggcaaa aattgccgcc attctcgaag
     6421 agccgccacg gatgggcaat agtgacctgg gcgtggcgtt ttcgcgcaat caaccagcct
     6481 ggcagcaacg tagtcagcaa ctgttaaaac gcttaaacgt acgcggcggt gaggcagaca
     6541 gttcgcttat cgcgccgcta cttgccgggg cgtttgccga tcgcattgct catcgccgtg
     6601 ggcaagatgg acgctatcaa ctggcaaaca gcatgggagc gatgctcgat gccgacgacg
     6661 cgctaagccg ccacgaatgg ttgatcgcac cgttattatt gcagggcagc gcctcgccgg
     6721 atgcgcggat tttactggcg ctaccggtcg atattgatga attagtacaa cgctgtccgc
     6781 agctggtgca gcaatctgac accgtggaat gggatgacgc gcaaggtacg ctgaaagcct
     6841 ggcgtcggct acaaatcggt cagttgacgg tgaaagtgca gccgctggcg aaaccgtcag
     6901 aagacgagtt gcatcaggcg atgcttaatg gcatccgtga taaaggttta agcgtgctca
     6961 actggacggc ggaagcggaa cagctacgct tgcgtttgtt atgcgccgca aagtggttgc
     7021 cggaatatga ctggccagcg gttgatgatg aaagtttgtt ggcgacgctg gaaacgtggc
     7081 tgctgccaca tatgaccggc gtacattcac tacgcggcct gaaatcactc gacatttatc
     7141 aggcactacg cggattactt gattggggaa tgcagcaacg tctggatagt gaattgcctg
     7201 cgcattacac tgtgccgacg ggaagccgga tcgccattcg ttatcatgaa gataacccgc
     7261 ccgcgctggc ggtgagaatg caggagatgt ttggtgaggc cactaatccg acgatcgccc
     7321 aggggcgcgt gccgctggtg ctgaagttgc tttcccctgc ccaaaggccg ctgcaaatca
     7381 cgcgtgattt gggggccttc tggaaagggg cgtatcgtga ggtgcaaaag gagatgaaag
     7441 ggcgttatcc caaacacgtc tggccggacg acccggcaaa cacggcaccg acgcgacgga
     7501 cgaaaaagta ttcgtaaggt tggttttctc cctctccctg tgggagaggg ccggggtgag
     7561 ggcatcagcg cgcacgttca ccctcaccct aaccctctcc ctcaagggag aggggaccga
     7621 tcgagcgcaa attttgagag atatcttctt ctgtcttgta acagaagaac agaaaatcgg
     7681 gcttttgcgc ctgaatattg cggagaaaaa gcatggccgg gaatgaccgc gagccaattg
     7741 gacgcaaagg gaaaccgacg cgtccggtca aacaaaaggt aagccgtcgt cgttacgaag
     7801 atgacgatga ttacgacgat tatgatgact atgaggatga agaaccgatg ccgcgcaaag
     7861 gtaagggcaa aggcaaaggg cgtaagcctc gtggcaaacg cggctggcta tggctactgc
     7921 taaaactggc tatcgttttt gccgtgctga tcgccattta cggcgtttat ctcgatcaaa
     7981 aaattcgtag ccgtattgat ggcaaggtct ggcaactgcc cgcggcagtt tatggccgaa
     8041 tggtcaacct tgaaccagat atgaccatca gcaagaacga aatggtgaag ctgctggagg
     8101 caacacagta tcgtcaggtg tcgaaaatga cccgtcctgg cgaatttacc gtgcaggcca
     8161 acagcattga gatgatccgc cgtccgtttg atttcccgga cagtaaagaa gggcaggtgc
     8221 gtgcgcgtct gacctttgat ggtgatcatc tggcgaccat cgtcaatatg gagaacaacc
     8281 gtcagttcgg tttcttccgt cttgatccgc gtctgatcac catgatctcc tcgccaaacg
     8341 gtgagcagcg tttgtttgtg ccgcgcagtg gtttccctga tttgctggtg gatactttgc
     8401 tggcgacaga agaccgtcat ttttacgagc atgatggaat cagtctctac tccattgggc
     8461 gtgcggtgct ggcaaacctg accgcaggtc gcacggtgca gggtgcgagt acgctgacgc
     8521 aacagctggt gaaaaacctg ttcctctcca gcgagcgttc ttactggcgt aaagcgaacg
     8581 aagcttacat ggcgctgatc atggacgcgc gttacagcaa agaccgtatt cttgagctgt
     8641 atatgaacga ggtgtatctc ggtcagagcg gcgacaacga aatccgcggc ttcccgctgg
     8701 caagcttgta ttactttggt cgcccggtag aagagctaag cctcgaccag caggcgctgt
     8761 tagtcggtat ggtgaaaggg gcgtccatct acaacccgtg gcgtaacccg aaactggcgc
     8821 tggagcgacg taatctggtg ctgcgtctgc tgcaacagca acagattatt gatcaagaac
     8881 tctatgacat gttgagtgcc cgtccgctgg gggttcagcc gcgtggtggg gtgatctctc
     8941 ctcagccagc ctttatgcag ctggtgcgtc aggagttgca ggcaaaactg ggcgataagg
     9001 taaaagatct ctccggcgtg aagatcttca ctacctttga ctcggtggcc caggacgcgg
     9061 cagaaaaagc cgccgtggaa ggcattccgg cactgaagaa acagcgtaag ttgagcgatc
     9121 ttgaaactgc gattgtggtc gtcgaccgct ttagtggtga agttcgtgcg atggtcggag
     9181 gttctgagcc gcagtttgcg ggctacaacc gtgcgatgca ggcgcgtcgt tcgattggtt
     9241 cccttgcaaa accagcgact tatctgacgg ctttaagcca gccgaaaatc tatcgcctga
     9301 acacgtggat tgccgatgcg ccaatcgcgc tgcgtcagcc gaatggccag gtctggtcac
     9361 cgcagaacga tgaccgtcgt tatagcgaaa gcggcagagt gatgctggtg gatgcgttga
     9421 cacgttcgat gaacgtgccg acggtaaatc tggggatggc gctggggctg cctgcggtta
     9481 cggagacctg gatcaaactg ggcgtaccga aagatcagtt gcatccggtt ccggcaatgc
     9541 tgctgggggc gttgaactta acgccaatcg aagtggcgca ggcattccag accatcgcca
     9601 gcggtggtaa ccgtgcaccg ctttctgcac tgcgttcggt aatcgcggaa gatggtaagg
     9661 tgctgtatca gagcttcccg caggcagaac gcgctgttcc ggcgcaggcg gcgtatctga
     9721 cactatggac catgcagcag gtggtacaac gtggtacggg tcgtcagctt ggggcgaaat
     9781 acccgaacct gcatctggca gggaaaacag ggactaccaa caataacgta gatacctggt
     9841 ttgcaggcat tgacggcagc acggtgacca tcacctgggt aggccgtgat aacaaccagc
     9901 cgaccaaact gtatggagcc agcggggcaa tgtcgattta tcagcgttat ctggctaacc
     9961 agacgccaac gccgctgaat cttgttccgc cagaagatat tgcagatatg ggcgtggact
    10021 acgacggcaa ctttgtttgc agcggtggca tgcgtgtctt gccggtctgg accagcgatc
    10081 cgcaatcgct gtgccagcag agcgagatgc agcagcagcc gtcaggcaat ccgtttgatc
    10141 agtcttctca gccgcagcaa cagccgcaac agcaacctgc tcagcaagag cagaaagaca
    10201 gcgacggtgt agccggttgg atcaaggata tgtttggtag taattaacat ctaagcgtga
    10261 aataccggat ggcgagttgc catccggtaa aataacatcc catctaagat attaaccctt
    10321 tcttttcatc tggttgttta ttaacccttc aggaacgctc agattgcgta ccgcttgcga
    10381 acccgccagc gtttcgaata ttatcttatc tttataataa tcattctcgt ttacgttatt
    10441 attcacttta catcagagat ataccaatgg cgcgttccaa aactgctcag ccaaaacact
    10501 cactgcgtaa aatcgcagtt gtagtagcca cagcggttag cggcatgtct gtttatgcac
    10561 aggcagcggt tgaaccgaaa gaagacacta tcaccgttac cgctgcacct gcgccgcaag
    10621 aaagcgcatg ggggcctgct gcaactattg cggcgcggca gtcagctacc ggcactaaaa
    10681 ccgatacgcc gattcaaaaa gtgccacagt ctatttctgt tgtgaccgcc gaagagatgg
    10741 cgctgcatca gccgaagtcg gtaaaagaag cgcttagcta cacgccgggt gtctctgttg
    10801 gtacgcgtgg cgcatccaac acctatgacc acctgatcat tcgcggtttt gcggcagaag
    10861 gccaaagcca aaataactat ctgaatggcc tgaagttgca gggcaacttc tataacgatg
    10921 cggtcattga cccgtatatg ctggaacgcg ctgaaattat gcgtggcccg gtttccgtgc
    10981 tttacggtaa aagcagtcct ggcggtctgt tgaatatggt cagcaagcgt ccgaccaccg
    11041 aaccgctgaa agaagttcag tttaaagccg gtactgacag cctgttccag actggttttg
    11101 actttagcga tgcgttggat gatgacggtg tttactctta tcgcctgacc ggtcttgcgc
    11161 gttctgccaa tgcccagcag aaagggtcag aagagcagcg ttatgctatt gcaccggcgt
    11221 tcacctggcg tccggatgat aaaaccaatt tcaccttcct ttcttacttc cagaacgagc
    11281 cggaaaccgg ttattacggc tggttgccga aagagggaac cgttgagccg ctgccgaacg
    11341 gtaagcgtct gccgacagac tttaatgaag gggcgaagaa caacacctat tctcgtaatg
    11401 agaagatggt cggctacagc ttcgatcacg aatttaacga cacctttact gtgcgtcaga
    11461 acctgcgctt tgctgaaaac aaaacctcgc aaaacagcgt ttatggttac ggcgtctgct
    11521 ccgatccggc gaatgcttac agcaaacagt gtgcggcatt agcgccagcg gataaaggcc
    11581 attatctggc acgtaaatac gtcgttgatg atgagaagct gcaaaacttc tccgttgata
    11641 cccagttgca gagcaagttt gccactggcg atatcgacca caccctgctg accggtgtcg
    11701 actttatgcg tatgcgtaat gacatcaacg cctggtttgg ttacgacgac tctgtgccac
    11761 tgctcaatct gtacaatccg gtgaatcccg atttcgactt caatgccaaa gatccggcaa
    11821 actccggccc ttaccgcatt ctgaataaac agaaacaaac gggcgtttat gttcaggatc
    11881 aggcgcagtg ggataaagtg ctggtcaccc taggcggtcg ttatgactgg gcagatcaag
    11941 aatctcttaa ccgcgttgct gggacgaccg ataaacgtga tgacaaacag tttacctggc
    12001 gtggtggtgt taactacctg tttgataatg gtgtaacacc ttacttcagc tatagcgaat
    12061 cgtttgaacc ttcttcgcaa gttgggaagg atggtaatat tttcgcaccg tctaaaggta
    12121 agcagtatga agtcggcgtg aaatatgtac cggaagatcg tccgattgta gttactggtg
    12181 ccgtgtataa tctcactaaa accaacaacc tgatggcgga ccctgagggt tccttcttct
    12241 cggttgaagg tggcgagatc cgcgcacgtg gcgtagaaat cgaagcgaaa gcggcgctgt
    12301 cggcgagtgt taacgtagtc ggttcttata cttacaccga tgcggaatac accaccgata
    12361 ctacctataa aggcaatacg cctgcacagg tgccaaaaca catggcttcg ctgtgggctg
    12421 actatacctt ctttgacggt ccgctttcag gtctgacgct gggcaccggt ggtcgttata
    12481 ctggctccag ctatggtgat ccggctaact cctttaaagt gggaagttat acggtcgtgg
    12541 atgcgttagt gcgttatgat ctggcgcgag tcggcatggc gggctccaac gtggcgctgc
    12601 atgttaacaa cctgttcgat cgtgaatacg tcgccagctg ctttaacact tatggctgct
    12661 tctggggcgc agaacgtcag gtcgttgcaa ccgcaacctt tcgtttctaa tttctctttt
    12721 ggggcacgga tttccgtgcc catttcacaa gttggctgtt atgcaggaat acacgaatca
    12781 ttccgatacc acttttgcac tgcgtaatat ctcctttcgt gtgcccgggc gcacgctttt
    12841 gcatccgctg tcgttaacct ttcctgccgg gaaagtgacc ggtctgattg gtcacaacgg
    12901 ttctggtaaa tccactctgc tcaaaatgct tggtcgtcat cagcctccgt cagaagggga
    12961 gattcttctt gatgcccagc cgctggaaag ctggagcagc aaagcgtttg cccgcaaagt
    13021 ggcttatttg ccgcagcagc ttcctccggc agaagggatg accgtgcgtg aactggtggc
    13081 gattggtcgt tacccgtggc atggcgcgct ggggcgcttt ggtgcggcag atcgcgaaaa
    13141 ggtcgaggaa gctatctcgc tggttggctt aaaaccgctg gcgcatcggc tggtcgatag
    13201 tctctctggc ggcgaacgtc agcgggcgtg gatcgccatg ctggtggcgc aggatagccg
    13261 ttgtctgttg ctcgacgaac cgacttcagc gctggatatc gcccaccagg ttgatgtgct
    13321 ggcgctggtg caccgtttaa gtcaggaacg tggcctgacg gtcattgccg tgttgcacga
    13381 tatcaatatg gcggcacgct actgtgatta tctggtcgcc ctgcgcagtg gtgaaatgat
    13441 tgctcaggga acgcctgcgg aaattatgcg cggcgaaacc ctcgaaatga tttatggcat
    13501 cccgatgggt attttgccgc atccggcggg tgctgcacct gtgagttttg tttattgatg
    13561 agcggcttac ctcttatttc gcgccgtcga ctgttaacgg cgatggcgct ttctccgttg
    13621 ttatggcaga tgaataccgc ccacgcggcg gctattgacc ccaatcgtat tgtggcgctg
    13681 gagtggttgc cggtggaatt actgctggcg ctcggcatcg tgccttacgg cgtggcggat
    13741 accatcaact atcgcctgtg ggtcagcgaa ccaccattgc cggactcagt gattgacgtc
    13801 ggtttgcgta cagaacctaa ccttgaactg ctgaccgaaa tgaaaccgtc gtttatggtc
    13861 tggtcggcag gatatggccc ttcatcagaa atgctggcac gtattgcgcc gggtcgcgga
    13921 tttaacttca gtgacggcaa acatccgttg gcgatggcgc gtaaatcgct gacggaaatg
    13981 gcagatttac ttaacctgca aagcgcagcg gaaacgcatt tagcgcaata cgaagacttt
    14041 atccgcagca tgaaaccccg ctttgtgaag cgtggtgcac gcccgttact gctgacgacg
    14101 cttatcgatc cgcgccatat gctggtcttc ggtccaaaca gcttgttcca ggaaattctc
    14161 gatgagtacg gcatcccaaa tgcctggcaa ggggaaacca acttctgggg cagtaccgcc
    14221 gtcagtatcg atcgtctggc agcgtataaa gacgttgatg tgctctgttt tgatcacgac
    14281 aacagcaaag acatggatgc gctaatggca accccgctgt ggcaggccat gccgtttgtc
    14341 cgtacagggc gctttcagcg cgtacccgca gtctggtttt atggtgcaac gctttcggca
    14401 atgcactttg tgcgtgttct ggataacgcc atcggaggta aagcgtgagt aaacgaattg
    14461 cgcttttccc ggcgttattg ctggcgctgt tagtgattgt cgctacggcg ctcacctgga
    14521 tgaacttctc acaggcgctg ccgcgcagtc agtgggcgca ggccgcctgg tcgccggata
    14581 ttgacgtcat cgagcagatg atttttcact acagcttgtt gccgcgtctg gcgatttcgc
    14641 tgctggtggg cgcgggcctg gggctggtgg gcgtgctgtt tcagcaagta ctgcgtaacc
    14701 cgctggcgga accgacgacg ttgggcgttg caacaggcgc gcaactgggg attaccgtca
    14761 ccacgctctg ggcgatccct agcgcgatgg cgagccagtt tgctgcgctg gcaggggctt
    14821 gcgttgttgg cttaattgtc tttggcgtcg cgtgggggaa acggctgtcg ccggtaacgc
    14881 tgattttcgc ggggttggta gtgagccttt attgcggcgc aatcaatcag ttactggtta
    14941 tcttccatca tgaccagttg caaagcatgt ttctgtggag cactggaacg ctgacgcaaa
    15001 ccgactgggg cggtgttgag cgtttatggc cgcagttgct gggcggtgtg atgctgacgt
    15061 tgctgctact tcgtccgtta accctgatgg ggcttgatga tggcgtggcg cgcaatctcg
    15121 ggctggcctt gtcgcttgcg cgtctggcgg cgctgtcgct ggcgattgtc atcagtgcgc
    15181 tgctggtgaa cgctgtgggg attatcggct ttatcgggtt gttcgcgccg ctgctggcaa
    15241 aaatgctggg ggcgcggcgt ctgctgccac gactgatgct ggcgtcgttg attggtgcgc
    15301 tgatcctctg gctttccgat caaatcatcc tctggctgac tcgcgtgtgg atggaagtgt
    15361 ccaccggttc ggtcactgcg ttgatcggtg cgccgctgct actgtggctg ctgccgcgtt
    15421 tacgcagcat tagcgcgccg gatatgaagg ttaacgatcg tgtcgcgact gaacgccaac
    15481 atgtgctggc gtttgccctc gcgggcggcg tgctgttgtt gatggctgtg gtggtggcgc
    15541 tgtcgtttgg tcgtgatgcg cacggctgga cgtgggcgag cggggcgttg ctcgaggatt
    15601 taatgccctg gcgctggccg cgaattatgg cggcgctgtt tgcgggcgtc atgctggcgg
    15661 tagcgggctg tattattcag cgactgaccg gcaacccgat ggcaagcccg gaagtgctgg
    15721 ggattagctc cggcgcggcg tttggtgtgg tgttaatgct gtttctggta ccgggtaatg
    15781 cctttggctg gttgttaccc gcaggcagtc tcggggcggc ggtgacgctg ttgatcatta
    15841 tgatcgccgc cggacgcggt ggattttccc cacaccgcat gttactggcg gggatggcgt
    15901 taagtaccgc gttcaccatg cttttgatga tgttgcaggc aagtggtgac ccgcgaatgg
    15961 cgcaagtgct gacctggatt tccggttcga cctacaacgc gaccgatgct caggtctggc
    16021 gcaccggaat tgtgatggtg attttgctgg cgattactcc gctgtgccgc cgctggctga
    16081 ccattttacc gctgggtggt gataccgcgc gggcggtagg gatggcgctg acgccgacgc
    16141 gaattgcgct gctgctgtta gcggcttgcc tgacggcgac cgcgacgatg actattggac
    16201 cgttgagttt tgttggttta atggcaccgc atattgcgcg gatgatgggc tttcgacgga
    16261 cgatgccaca catcgtaatt tcggcgctgg tgggtggttt actgctggtg ttcgctgact
    16321 ggtgtgggcg gatggtgctg tttccattcc agatcccggc ggggctgctg tcgaccttta
    16381 tcggtgcgcc atattttatc tatttgctga gaaagcagag ccgttaatag aatgcctcgc
    16441 ccggtgggcg aggcaaaatt atcacaactt cgcgaacacc cgacgtgcag catcgatggt
    16501 gttattgata tcttccatgc tgtgcgccac agacataaag cccgcttcaa acgctgacgg
    16561 tgccaggtaa acaccttcat ccagcatcat atggaagaaa cgcttaaaac gctcgacgtc
    16621 acaggccatc acatcctgat agcacgtcac ggactcggcg tcggtaaaga agataccgaa
    16681 cataccaccg acgtggttaa cgaccagcgg aattcccgct tcttctgccg cttccagcag
    16741 accttctgcc agacgtgttg tcagctcatc cagcgtttcg tgaatgcccg gctgcgcgac
    16801 ttcattcaga caggcgaaac ccgctgccat cgcaatcggg ttaccggaaa gcgtacccgc
    16861 ctgatagacc ggacctgtcg gggccagcgc atccattaca tcacgacgac caccgaatgc
    16921 gcctaccggc attccaccgc cgatgatttt tccgaggcag gttaaatctg gcactacggc
    16981 gaaataatcc tgtgcgccag ctagcgctac gcggaaaccg gtcatcactt catcgatgat
    17041 cagcaacgcg ccaaattcgt cgcacagcgc acgcagacct ggcaggaact ctggcagcgg
    17101 cggaacacag ttcatattgc ctgccaccgg ctcgacgata atacaggcaa tctcttgcgg
    17161 gtattgctca aacgcggcgc gtacagaggc cagatcgtta taagtacagg ttaaggtatg
    17221 tttggcgaaa tctgccggaa cgcccggcga gtttggctgg cctaacgtga gtgcgccaga
    17281 accggctttc accagcaggc agtcagcgtg accgtggtaa caaccttcaa atttaatgat
    17341 tttgtcgcga ccggtaaaac cacgggccag gcggatggcg ctcatcgtcg cctcggtgcc
    17401 ggagttcacc atgcgcacca tatccatggt cgggaccagt tcggtcacca gttgcgccat
    17461 tttcacttcc atttcggtcg gtgcaccaaa gcttaaacca cgctcggcgg cttcaatcac
    17521 ggcattgcgg atcgccggat ggttatggcc cagcaccatc ggcccccagg aaccgacata
    17581 atcgatatag gctttgccat caacatcgta cagataagcg ccgtccgctt tttcgataaa
    17641 cagtggagtg ccgcccacgc cagtaaaggc gcgaacaggg gagttcacac cgccagggat
    17701 cagctcgcgc gctgcgctgt aaagattttc agacttactc atggagggtt cctgattcgt
    17761 agaaaaagtg aatggctgct attccatgtt attcataaca agttaaatac tcgtcaaaca
    17821 tcaggctgct tgtactggtg caatcctgat ttcttagagt ataaaagttt tgtgcatttg
    17881 aaacattacg ctttgcaaag gattttcatg gaacgtgcga gtaaaatgcc gtcatcttat
    17941 ttgtatgacc aataagtgat cattggatga aaactgatac tccctcttta gaaacaccgc
    18001 aggccgcgcg cctgcgacgc agacaactga ttcgccaact tcttgagcgc gataaaaccc
    18061 cgttagccat tttgtttatg gcggcagtcg tcggcacgct tgtcgggctg gctgcggttg
    18121 cttttgacaa aggtgtcgcc tggttgcaga accaacgtat gggggcgctg gtacatactg
    18181 ctgataatta tccgctactg ttaaccgtcg cttttctctg ttcggcggtg ctggcgatgt
    18241 tcggctactt tttggtgcgc aaatacgcgc ccgaagcagg gggatcgggg atcccggaaa
    18301 ttgaaggggc gctggaagat caacgtcctg ttcgctggtg gcgtgtattg ccggtgaaat
    18361 tctttggcgg gctggggaca ctcggcggag gcatggggct agggcgcgaa ggaccaaccg
    18421 tgcagatcgg cggcaatatc agtcgcatgg tgcttgatat tttccgcctg aaaggtgatg
    18481 aagctcgcca tacgctgctg gcaaccggtg ctgctgcggg gctggctgcg gcctttaacg
    18541 cgccgctggc gggtattttg tttattatcg aagagatgcg tccgcagttt cgctatacgt
    18601 taatttcgat taaagcggta tttattggcg tcattatgtc gaccattatg taccggattt
    18661 ttaatcatga agttgcgttg attgacgtcg gtaaactttc tgatgcgccg cttaatacgc
    18721 tgtggcttta tctgatcctc ggtattattt ttggcatttt cggccctatt tttaataaat
    18781 gggtgctggg gatgcaggat ttgctgcacc gtgtgcacgg cggcaatatt accaaatggg
    18841 tactgatggg gggtgcgatt ggcggtctgt gtggattgct ggggtttgtg gcaccagcaa
    18901 cgtcgggcgg cggttttaac ctgattccta tcgctaccgc ggggaatttc agcatgggaa
    18961 tgctggtgtt tatcttcgtc gctcgggtca ttaccacctt actctgcttc tcttccggcg
    19021 cgccgggcgg tatttttgcc ccgatgctgg cgctgggtac tgtgctggga accgctttcg
    19081 gaatggttgc cgttgagctg tttccgcaat atcacctgga ggcggggacg tttgctattg
    19141 ccggaatggg ggcattactg gcggcatcta ttcgcgcgcc gttaacgggg atcattctgg
    19201 ttctggagat gaccgataac taccagctca ttttgccaat gattattacc ggtcttggcg
    19261 caacactatt agcgcaattt accggcggga aacctctata ctcggcgatt cttgcgcgca
    19321 cgctggcaaa acaggaagct gagcaactgg cgcgaagcaa ggccgcatca gccagcgaga
    19381 atacttgaac gaaataccag ggtattagat aatggcgatt attattgggt tagaatttgc
    19441 ccgattgccg atgtcgtttg gagcaaaata tgagtgatga cgtagcactg ccgctggagt
    19501 ttaccgacgc agcagccaac aaagttaaaa gcctgatcgc tgacgaagat aacccgaatc
    19561 tgaaattacg cgtgtatatc accggtggcg gttgcagcgg cttccagtat ggtttcacct
    19621 ttgatgatca ggtgaacgaa ggcgatatga ccatcgaaaa acagggcgtt ggcctggtgg
    19681 ttgatccgat gagcctgcaa tatctggtcg gcggttccgt tgactacacc gaaggactgg
    19741 aaggttctcg tttcatcgtg accaacccga acgcgaaaag cacctgcggt tgcggttctt
    19801 cctttagcat ctaatctgtt gttcatgtgc cggataaact atccggcaca tcttctctct
    19861 tcaacgccca ttctcatcca gcgcaaacgt cggtagctta agatgccaac gaatagccgc
    19921 cagccgaatc aatagcgtca cgaccatgcc catcatactg gctgtttcca gtggtacgga
    19981 aaatgtgtaa taagccgtag cgtggacaat accgccgata atacaggcag ttgcgtagat
    20041 ttctgtacgt aaaatcatgg ggatttcgcg gaccagtaca tcacgaatga tcccgccgcc
    20101 aacgccagta atgacgccca tacaaaccgc gattaacgga ccggcttccg cattaaaggc
    20161 tttattcacg ccaatgccga caaacaccgc cagaccaacg gcgtccagca ccggcaacat
    20221 ccattttggt aagcgtcttg gctggcgcac cagcacgatg gtcagcatgc tggtgaccat
    20281 ggcaacgacc agatcggtgg gatctttcac ccaaaatacc gggccgtgat ccagcgccat
    20341 gtcgcgaatt gttccgtcgc ctactgcggt aaccacgccc agtaccagaa caccaaaagg
    20401 gtccatacgc aattttccgg ctaacaaaac gccgtagatg gcaaataccg ctgtgccgac
    20461 tatatccagc caatagacga gcattgttca atccccactg agcacctgaa aaggtcaggc
    20521 actaatctac ctgtgaaagc gcattacaga gctgttgtgc tgcgaggata atacgtgggc
    20581 ttgcacgttc aaaccagtca ctcgtgagag gaataacggg aattttgagc tgttcacccc
    20641 agtattgttt gattttagga atttggtccg gtccgcctgt aatgacaata gcctgtggcg
    20701 agcgtgctaa cacctgttcg cggctaactt gcggccaggg aacccggctg tctttaaaga
    20761 tgttttctcc gccacaaact tcgagtacct ggttctgaat cgactctttt ccactggtaa
    20821 atggcggatt aatgccgaat tgcagaaaaa cacgtttttt aggtttatca gcatattgcg
    20881 ctttcaattg cacgtactga tccagcaggg attgcgcggc ttgttcggcc ttgtctggtt
    20941 gcggactcca gggggccagt tgacgtaacg cattggcaat ttgttcaatg cttgtcgcat
    21001 cgacccacat cacttttatt cccagcgaag ccagctggtc aacctgccgc tcggcattac
    21061 ctccacgcca ggcaatcacc agatcgggtt tcagcgcgac aatgcgttcc agattcatcc
    21121 cctgccaggt ggaaacctgc tcaatctttt gcgcttgtgg aggatagtcg gaatagctgc
    21181 tgaccccaac cggcgtgatc ccggcggcaa aggcaagttc agtgttggcg ggagaaagcg
    21241 tgatgacgcg cggcgcggcg ttgagccaca gtggcgcgag aaaagacagg gcgaccagcg
    21301 ccctgaacag tgatttagcc atgtgccagt ttctgcacca gtgactcaac catcaggctg
    21361 gactgtttag cggcaacagc caggaactca tcgaagctaa gatgagactg ttgatcggcc
    21421 acgtcggaga tggcgcgtac gacaacaaac gggacgttga aattgtggca gacatgggcg
    21481 attgccgtcg cttccatctc tacagcaatg gcctgtggga agttgtggcg gattttcgcc
    21541 agaccaacag aaccgttgat gaaagcgtcg ccgctaacaa tcaggccacg tacagcgtta
    21601 agattcagtt cggcaatgca ggcctcagcg gcagcgatca gtttatcgtc agctttaaag
    21661 cctgccggac agcctggtaa ctgaccgtat tcataaccaa atgccgtgac atccgcgtcg
    21721 tgataacgcg cttcgtccga gacaacgata tcgcccactt tcagcgttgg tgccaggcca
    21781 ccggcagaac cggtgttaat aatcacatct ggcttgcagt gttccaacag cagagtggca
    21841 cccagcgccg cagcgacttt accgatgccc gatttcagaa gcgcaacctc ggttccattc
    21901 agttggccgg tatagatttc gcaaccgccg agactgatag tttgacggtt ttcgattttg
    21961 tcacgcagca gcgtaacttc ttcttccatt gcaccaatga tgccgatttt catagattta
    22021 ctcgcgataa gcccgatttg aaggcatagt ttaccatgcg cttacgggga agcgtatttc
    22081 tcacgcggga gaggacatgg cacagattga tttccgaaaa aaaataaact ggcatcgtcg
    22141 ttaccgttca ccgcagggcg ttaaaaccga acatgagatc ctgcggatct tcgagagcga
    22201 tcgcgggcgt atcatcaact ctccggcaat tcgtcgtctg caacaaaaga cccaggtttt
    22261 tccactggag cgcaatgccg ccgtgcgcac gcgtcttacc cactcgatgg aagtccagca
    22321 ggtgggacgc tacatcgcca aagaaatttt aagccgtctg aaagagctta aattgctgga
    22381 agcatacggc ctggatgaac tgaccggccc ctttgaaagc attgttgaga tgtcatgcct
    22441 gatgcacgat atcggcaatc cgccgtttgg tcattttggc gaagcagcga taaatgactg
    22501 gtttcgccaa cgtttgcacc cggaagatgc cgaaagccag cctctgactg acgatcgctg
    22561 cagcgtggcg gcactacgtt tacgggacgg ggaagaaccg cttaacgagc tgcggcgcaa
    22621 gattcgtcag gacttatgtc attttgaggg gaatgcacaa ggcattcgtc tggtgcatac
    22681 attgatgcgg atgaatctca cttgggcaca ggttggcggt attttaaaat atacccgtcc
    22741 ggcgtggtgg cgtggcgaaa cgcctgagac acatcactat ttaatgaaaa agccgggtta
    22801 ttatctttct gaagaagcct atattgcccg cttgcgtaaa gaacttaatt tggcgcttta
    22861 cagtcgtttt ccattaacgt ggattatgga agctgccgac gacatctcct attgtgtggc
    22921 agaccttgaa gatgcggtag agaaaagaat atttaccgtt gagcagcttt atcatcattt
    22981 gcacgaagcg tggggccagc atgagaaagg ttcgctcttt tcgctggtgg ttgaaaatgc
    23041 ctgggaaaaa tcacgctcaa atagtttaag ccgcagtacg gaagatcagt tttttatgta
    23101 tttacgggta aacaccctaa ataaactggt accctacgcg gcacaacgat ttattgataa
    23161 tctgcctgcg attttcgccg gaacgtttaa tcatgcatta ttggaagatg ccagcgaatg
    23221 cagcgatctt cttaagctat ataaaaatgt cgctgtaaaa catgtgttta gccatccgga
    23281 tgtcgagcag cttgaattgc agggctatcg ggtcattagc ggattattag agatttatcg
    23341 tcctttatta agcctgtcgt tatcagactt tactgaactg gtagaaaaag aacgggtgaa
    23401 acgtttccct attgagtcgc gcttattcca caaactctcg acgcgccatc ggctggccta
    23461 tgtcgaggct gtcagtaaat taccgtcaga ttctcctgag tttccgctat gggaatatta
    23521 ttaccgttgc cgcctgctgc aggattatat cagcggtatg accgacctct atgcgtggga
    23581 tgaataccga cgtctgatgg ccgtagaaca ataaccaggc ttttgtaaag acgaacaata
    23641 aatttttacc ttttgcagaa actttagttc ggaacttcgg gctataaaac gaatctgaag
    23701 aacacagcaa ttttgcgtta tctgttaatc gagactgaaa tacatgaaaa aaaccacatt
    23761 agcactgagt gcactggctc tgagtttagg tttggcgtta tctccgctct ctgcaacggc
    23821 ggctgagact tcttcagcaa cgacagccca gcagatgcca agccttgcac cgatgctcga
    23881 aaaggtgatg ccttcagtgg tcagcattaa cgtagaaggt agcacaaccg ttaatacgcc
    23941 gcgtatgccg cgtaatttcc agcagttctt cggtgatgat tctccgttct gccaggaagg
    24001 ttctccgttc cagagctctc cgttctgcca ggggggccag ggcggtaatg gcggcggcca
    24061 gcaacagaaa ttcatggcgc tgggttccgg cgtcatcatt gatgccgata aaggctatgt
    24121 cgtcaccaac aaccacgttg ttgataacgc gacggtgatt aaagtccagc tgagcgatgg
    24181 tcgtaagttc gacgcgaaga tggttggcaa agatccgcgc tctgatatcg cgctgatcca
    24241 aatccagaac ccgaaaaaac tgaccgcaat taagatggcg gattctgatg cactgcgcgt
    24301 gggtgattac accgtagcga ttggtaaccc gtttggtctg ggcgagacgg taacttccgg
    24361 gattgtctct gcgctggggc gtagcggcct gaatgctgaa aactacgaaa acttcatcca
    24421 gaccgatgca gcgatcaacc gtggtaactc cggtggtgcg ctggttaacc tgaacggcga
    24481 actgatcggt atcaacaccg cgatcctcgc accggacggc ggcaacatcg gtatcggttt
    24541 tgctatcccg agtaacatgg tgaaaaacct gacctcgcag atggtggaat acggccaggt
    24601 gaaacgcggt gagctgggta ttatggggac tgagctgaat tccgaactgg cgaaagcgat
    24661 gaaagttgac gcccagcgcg gtgctttcgt aagccaggtt ctgcctaatt cctccgctgc
    24721 aaaagcgggc attaaagcgg gtgatgtgat cacctcactg aacggtaagc cgatcagcag
    24781 ctttgccgca ctgcgtgctc aggtgggtac tatgccggtg ggcagcaaac tgaccctggg
    24841 cttactgcgc gacggtaagc aggttaacgt gaacctggaa ctgcagcaga gcagccagaa
    24901 tcaggttgat tccagctcca tcttcaacgg cattgaaggc gctgagatga gcaacaaagg
    24961 caaagatcag ggcgtggtag tgaacaacgt gaagacgggc actccggctg cgcagatcgg
    25021 cctgaagaaa ggtgatgtga ttattggtgc gaaccagcag gcagtgaaaa acatcgctga
    25081 actgcgtaaa gttctcgaca gcaaaccgtc tgtgctggca ctgaacattc agcgcggcga
    25141 cagcaccatc tacctgttaa tgcagtaatc tccctcaacc ccttcctgaa tacgggaagg
    25201 ggttctcctt acaatctgtg aacttcgcca caactccata catcttcatc atcctttggg
    25261 catttgcaca atgccgtacg ttacgtactt ccttatgcta agccgtgcat aacggaggac
    25321 ttatggctgg ctggcatctt gataccaaaa tggcgcagga tatcgtggca cgtaccatgc
    25381 gcatcatcga taccaatatc aacgtaatgg atgcccgcgg gcgaattatc ggcagcggcg
    25441 atcgtgagcg tattggtgaa ttgcacgaag gtgcattgct ggtactttca cagggacgag
    25501 tcgtcgatat cgatgacgcg gtagcacgtc atctgcacgg tgtgcggcag gggattaatc
    25561 taccgttacg gctggaaggt gaaattgtcg gcgtaattgg cctgacaggt gaaccagaga
    25621 atctgcgtaa atatggcgaa ctggtctgca tgacggctga aatgatgctg gaacagtcgc
    25681 ggttgatgca cttgttggcg caggatagcc gtttgcggga agaactggtg atgaacctga
    25741 ttcaggcaga ggagaatact cccgcacttc tgaatgggcg caacggctgg ggatcgatct
    25801 caatcaaccg cgagtggtgg ctattgttga ggtcgacagc ggtcagcttg gcgtggacag
    25861 cgcaatggcg gagttacaac aactgcaaaa cgcgctgact acgcccgagc gtaataatct
    25921 ggtggcgatt gtctcgctaa ccgaaatggt ggtgttgaaa ccggcgttga actcttttgg
    25981 gcgctgggat gcagaagatc atcgtaagcg agttgaacaa ctgattaccc gcatgaaaga
    26041 gtacggccag ctgcgttttc gcgtttcact gggcaactat tttaccggtc ctggcagtat
    26101 tgcccgatcc tatcgtacgg cgaaaacgac gatggtggtg ggtaaacagc ggatgccaga
    26161 aagtcgctgc tatttttatc aggatctgat gttacctgtg ttactcgaca gtttgcgtgg
    26221 cgactggcag gccaacgaac tggcgcgacc gctggcgcgg ctgaaagcga tggacaataa
    26281 cggcttgctg cgacgaacgc tggcggcgtg gtttcgccac aatgtgcaac cgctggcaac
    26341 gtcaaaggcg ttgtttattc atcgtaatac cctggagtat cggcttaatc gtatatcgga
    26401 actgaccggg cttgatttgg gcaattttga tgacaggttg ctgctgtatg tggcgttaca
    26461 actggatgaa gagcggtaga ttatgcgtta aggttgaggc agcgctggct cattcccctc
    26521 agcccgaagg ggagagggga atgcccgagt taaccgtcaa ttcgggatta tttacgcgtt
    26581 agtttttcca gatcggcttc aatctcgctg atcttattgg ttacgactga ctccaggtga
    26641 cgtaagtcgt cgaggatctt acgcttaaga tcaacttcgc tgcggtcacg ctgacagatt
    26701 tgatcaagct catcaatgat ataccgtaga ttcgggctga tttcctggac ttctttataa
    26761 ccctgaccca caccatcagc gacgaccgtt ttacgctgac gcggatattt aaacttaacg
    26821 ctcttggcga aaaactcgcc tttgtctttc tggaaataga ttttcagaat atcgttgttg
    26881 gcttcctgcc ggaggctgta acgatcaatt tcttcaggat tggtaatacc cagacttttc
    26941 agattgtcgt acatagcgtt acctcaaaat gagtcagtaa attgtgctta ttttagcatt
    27001 tggcttggcc cgcccggctc gactgtgatc gcagaaagcc tttttcttcc agacttctat
    27061 catggcgtaa aaatcaaaaa attacctgct ttattgtggc gatattccat gggatggatt
    27121 agcacattta tcttcgtttt gcgaattaca tgacagtccg aaacaagatg aaaaagtatt
    27181 taaaaaaggg ggaggaagaa tgagtaggac gaattattct gagccagaaa gcatacgggc
    27241 agcatcatgc tgcccgtata tgtactctta atcgatggta cgcagcagtt cgttaatgcc
    27301 gactttgccg cgagttttcg cgtcaacttt cttaacgata accgcacagt agaggctgta
    27361 tttgccatct tttgacggca gattacctga aacaaccaca gaccccgccg gaacgcgacc
    27421 gtagtggatt tcgccggttt cacggtcgta aatacgggtg ctctgaccaa tgtatacgcc
    27481 catagagatg acggaacctt cttcgacaat caccccttca accacttcag agcgtgcgcc
    27541 aatgaagcag ttatcttcaa taatggtcgg gttagcctgc agcggctcca gcacgccgcc
    27601 gatgcccacg ccaccggaaa ggtggacgtt tttaccaatc tgcgcacaag aaccgacggt
    27661 cgcccaggta tcaaccatgg tgccttcatc aacatatgcg ccgatgttaa cgtaagacgg
    27721 catcagcacg gtgttacggg caataaacgc accctgacgt accgccgctg gtggcacaac
    27781 gcggaagcct tctttctgga aacgtgcttc gtcgtagtcg gcgaatttca tcggcacttt
    27841 gtcgaagtag cggctttctg ccccttcgat cacctgatta tcattaatac ggaaagagag
    27901 cagcaccgct tttttcaacc actgatgcgt cacccactga ccgtcaattt tttccgctac
    27961 acgcagtgcg ccggaatcca gcagggcgat cacctgattt accgcttcgc gggtaacggt
    28021 gtctgcattg gctggcgtga tctcggcacg gcgttcaaaa gcggtttcaa taatgttctg
    28081 taactgctgc attgttaaac tcttttcata tcagtaaaca catcaccctt tatcgtttgg
    28141 attgagggcc tctgtcaacc gctgatgcac ttcctgctgc aactcgttat taagcgcacg
    28201 ccggtcagcg gtggcaatta tgaataaatc ttctactcgc tcgccaatgg ttgtaattcg
    28261 ggcaccatga agcgaaattc ccagatcggc aaaaattttc ccgactcgcg ccagcagtcc
    28321 aggttggtcg agggcgatca gttcgaggaa cgatttgcgg tcggtatggg tcggcaaaaa
    28381 cgttacttcg gtttcaacag taaaatggcg taatttggcg ggttggcgac ggggctgcgg
    28441 tggctgccag ctactttgcg tcagcacctg ctccagacca aaccgaataa cctcatggcg
    28501 atctgcggac agcgggctgc catcgggttc cagcacgata aaggtatcca tcgccatacc
    28561 gtcgcgagtg gtgaaaattt gtgcgtcgtg aacacttaaa ttgcggcggt ctaattcggc
    28621 acagacggcg gcaaacagat aggggcggtc cgggctccag ataaaaatct cggtgcctcc
    28681 acgcgtagcc tgcgggctaa gcaataccag cggtttgctt aaatcatgct gtaataaatg
    28741 gcgggcatgc caggccagtt gatttgggct atggcggaca aaatagttag cacgacagcg
    28801 tgaccaaatt tggtgcagcg cctcttcgtc gatgttatcc atgcgcagta gtgccagtgc
    28861 ctggagttgg tgatggcgaa cccgttcgcg catatccggc gtgttttgca ttccgcgtcg
    28921 tagctgcttt tcggtggcaa aatagagttc gcgcaacaga ctttgcttcc agctattcca
    28981 cagtgtttcg ttggtggcgc aaatgtcagc cactgtcagg cataccagat agcgcagacg
    29041 attttccgtt tgaacttctt cggcaaactg cttgatgact tcagggtcct gaatatcgcg
    29101 gcgttgtgct gtcaccgaca tcagcaagtg ctggcgaacc agccaggcaa ccagctgtgt
    29161 ttcgcgtgag ttcagcccgt ggagttctgc aaaatgcact acatcctgag caccgagaat
    29221 ggagtggtcg ccgccgcgtc ctttggcgat atcgtgaaac agcgcggcga tgaaaatcag
    29281 ctcagttgac ggcaggcgcg gccagacgtc cacacacaac ggatggcgct ggcgcgtttc
    29341 ttcactggca aaactctcca gtttcagcat cacgcggata gtatgttcat ccaccgtgta
    29401 ggcgtggaac agatcaaact gcatctgccc gacgatatgc gaccattgcg gcatatacgc
    29461 gccgagcacg ctatggcgat gcattggcaa tagtccgcgc cgcaccgctc cggggtgacg
    29521 cagaatgctc aaaaacagtt ttcgtgcttg cggaatatta cacagcggtt gttgcagatg
    29581 gcgacgggca tggcgtaact ggcgcagcgt ggtggagtaa atgccggtga tcgcactgtt
    29641 gcgcaccatg gtgtagaaca tacgcaagat ggcttccggc tggcgcataa atagtgtttc
    29701 atcacgcagg tcgattagcg taccgcgtag ctgaaactca tcgtcgagtg gacgtggttt
    29761 ttcgtcggcg ggaagggcga ggatggcttc atcgaacagt tgcagcagca tctggttgag
    29821 ttcactgacg cggcgtgtaa cgcggaagta atccttcatc atccgctcga ccggttcgtt
    29881 gccttcacca ctgtaattca gacgctgagc gacgctaagt tggcgatcga ataacagacg
    29941 attatcgtaa cggctgacga ccagatgcag ggcaaagcga atacgccaca gtatatgcag
    30001 acattcgttt aattccgccc gctccgctga ggttaagaag ccaaacccga ccatttcatc
    30061 cagcgatgtt gcgccaaaat gacggcgggc cacccattgc agagtgtgaa tatcgcgcaa
    30121 gccgccaggg ctacttttga tgtctggttc aaggttgtag ctggtgccat ggtaacgctg
    30181 atggcgctgg ttctgttctt caactttcgc tgcgtagaac ttgtcggaag gccagaatcc
    30241 ttcgctaaaa atatgttttt gcagttcgag gaagagcgca acatcgccaa ttaataagcg
    30301 ggattcgatt aaattggtgg cgacggttaa atccgataac ccttccaaca tgcactcttc
    30361 aagcgtgcgc acactatgac cgacttccag ctttacatcc cagagcagcg ttaacagctc
    30421 gcccactttt tgcgcctgat cgtccgggag ctttttacgg cttaaaatca gtaaatcgat
    30481 gtcagaaagt ggatgcagct cgccacgacc gtagccaccg acggcaacca atgccaggtc
    30541 ggcaatctgg ctgaatcccg cttcaatcca taatcgttgc aggagctggt ctatgaactc
    30601 ggtacgtgcc tcaatcaact gttctgcaga gatcccattg tcaaaggcat cacccagcca
    30661 acgctggaaa gtatcgatat gggcttttat cccacagacg gttaattcat cacggggcca
    30721 ggcgcatgga ttttgcggtt gaccgggcag ggtggagaga gcggtgtttg cgtactgttc
    30781 tggaagggta ttcattgtgc gccacccata agattaaatt atcgcattaa aaaagccggc
    30841 attcgccggc ttcatcttat tcgtcgtgcg agattatcgc cgggatggtg tcatccttgc
    30901 gtagcgtcaa aatttcgcag ccgttatcag tcaccacaat agtatgctca tattgtgcag
    30961 acaagctgcg atctttggtt tttaccgtcc agccatcttt catggtgcgg atctcttttt
    31021 tacccgcgtt gaccattggc tcaatggtga acgtcatccc cggtttcagc acgacgttgg
    31081 tttcacggga gtcatagtgc agcacctgcg gttcttcatg gaagccgcga ccaataccgt
    31141 gtccgcaata ttcacgaacg acggagaagc cttctgcttc gacaaatttc tgaatcgccg
    31201 caccgatttc gcgcagatta atgcctggtt ttaccatgcg tagcgccagg tacaggcttt
    31261 cttgcgtgat gcggcacagg cgttcgccca tgatggtcgg cttaccgacg ataaacattt
    31321 tcgaggtatc gccgtggaaa ccatctttga ttacggtgac atcaatgtta acgatatcgc
    31381 catctttcag cagcttagca tcgtccggga taccgtggca caccacttca ttaatagaga
    31441 tgcaaacgga tttcggatag ccgtgatagc cgaggcaggc agaaaccgcg tgttgttcat
    31501 taacaatgta atcattacag atgcgatcca gctcgccggt gctgacgccc ggtttaacat
    31561 acggttcgat catctccagc acttcggcag ccagtcggcc agcgacgcgc attttttcga
    31621 tatcttctgg ggtcttgatt gagatagcca ttaattctgt ccatcagcgt cggtgatacc
    31681 gacaatatat atgtaagtgc cgtcaatggt atcacacccg ggcaaattga gaatcattct
    31741 gaatttcgcc aaacgtgcca ctgaaggttt tctataatag aaaattcgac gtctgatgct
    31801 gtacacagcg ccaacaatta ttggtgtcca cgacgtattt gtggtataaa gcgcgccgga
    31861 cttccgatcc atttcgtata cacagactgg acggaagcga caatctcact ttgtgtaaca
    31921 acacacacgt atcggcacat attccggggt gccctttggg gtcggtaata tgggatacgt
    31981 ggaggcataa ccccaacttt tatatagagg ttttaatcat ggcaactgtt tccatgcgcg
    32041 acatgctcaa ggctggtgtt cacttcggtc accagacccg ttactggaac ccgaaaatga
    32101 agccgttcat cttcggtgcg cgtaacaaag ttcacatcat caaccttgag aaaactgtac
    32161 cgatgttcaa cgaagctctg gctgaactga acaagattgt ttctcgcaaa ggtaaaatcc
    32221 ttttcgttgg tactaaacgc gctgcaagcg aagcggtgaa agacgctgct ctgagctgcg
    32281 accagttctt cgtgaaccat cgctggctgg gcggtatgct gactaactgg aaaaccgttc
    32341 gtcagtccat caaacgtctg aaagacctgg aaactcagtc tcaggacggt actttcgaca
    32401 agctgaccaa gaaagaagcg ctgatgcgca ctcgtgagct ggagaaactg gaaaacagcc
    32461 tgggcggtat caaagacatg ggcggtctgc cggacgctct gtttgtaatc gatgctgacc
    32521 acgaacacat tgctatcaaa gaagcaaaca acttgggtat tccggtattt gctatcgttg
    32581 ataccaactc tgatccggac ggtgttgact tcgttatccc gggtaacgac gacgcaatcc
    32641 gtgctgtgac cctgtacctg ggcgctgttg ctgcaaccgt acgtgaaggc cgttctcagg
    32701 atctggcttc ccaggcggaa gaaagcttcg tagaagctga gtaataaggc ttgataactc
    32761 ccccaaaata gttcgagttg cagaaaggcg gcaagctcga gaattcccgg gagcttacat
    32821 cagtaagtga ccgggatgag cgagcgaaga caacgcatct gcggcgcgaa atatgaaggg
    32881 ggagagccct tatagaccag gtagtacacg tttggttagg gggcctgcat atggccccct
    32941 ttttcacttt tatatctgtg tggtttaatg ccgggcagat cacatctccg aggattttag
    33001 aatggctgaa attaccgcat ccctggtaaa agagctgcgt gagcgtactg gcgcaggcat
    33061 gatggattgc aaaaaagcac tgactgaagc taacggcgac atcgagctgg caatcgaaaa
    33121 catgcgtaag tccggtgcta ttaaagcagc gaaaaaagca ggcaacgttg ctgctgacgg
    33181 cgtgatcaaa accaaaatcg acggcaacta cggcatcatt ctggaagtta actgccagac
    33241 tgacttcgtt gcaaaagacg ctggtttcca ggcgttcgca gacaaagttc tggacgcagc
    33301 tgttgctggc aaaatcactg acgttgaagt tctgaaagca cagttcgaag aagaacgtgt
    33361 tgcgctggta gcgaaaattg gtgaaaacat caacattcgc cgcgttgctg cgctggaagg
    33421 cgacgttctg ggttcttatc agcacggtgc gcgtatcggc gttctggttg ctgctaaagg
    33481 cgctgacgaa gagctggtta aacacatcgc tatgcacgtt gctgcaagca agccagaatt
    33541 catcaaacca gaagacgtat ccgctgaagt ggtagaaaaa gaataccagg tacagctgga
    33601 tatcgcgatg cagtctggta agccgaaaga aatcgcagag aaaatggttg aaggccgcat
    33661 gaagaaattc accggcgaag tttctctgac cggtcagccg ttcgttatgg aaccaagcaa
    33721 aactgttggt cagctgctga aagagcataa cgctgaagtg actggcttca tccgtttcga
    33781 agtgggtgaa ggcatcgaga aagttgagac tgactttgca gcagaagttg ctgcgatgtc
    33841 caagcagtct taattatcaa aaaggagccg cctgagggcg gcttcttttt gtgcccatct
    33901 tgtaaattca gctaaccctt gtggggctgc gctgaaaagc gacgtacaat gtcgctagta
    33961 ttaattcatt tcaatcgttg acagtctcag gaaagaaaca tggctaccaa tgcaaaaccc
    34021 gtctataaac gcattctgct taagttgagt ggcgaagctc tgcagggcac tgaaggcttc
    34081 ggtattgatg caagcatact ggatcgtatg gctcaggaaa tcaaagaact ggttgaactg
    34141 ggtattcagg ttggtgtggt gattggtggg ggtaacctgt tccgtggcgc tggtctggcg
    34201 aaagcgggta tgaaccgcgt tgtgggcgac cacatgggga tgctggcgac cgtaatgaac
    34261 ggcttggcaa tgcgtgatgc actgcaccgc gcctatgtga acgctcgtct gatgtccgct
    34321 attccattga atggcgtgtg cgatagctac agctgggcag aagctatcag cctgttgcgc
    34381 aacaaccgtg tggtgatcct ctccgccggt acaggtaacc cgttctttac caccgactca
    34441 gcagcttgcc tgcgtggtat cgaaattgaa gccgatgtgg tgctgaaagc aaccaaagtt
    34501 gacggcgtgt ttaccgctga tccggcgaaa gattcaaccg caaccatgta cgagcaactg
    34561 acttacagcg aagtgctgga aaaagagctg aaagtcatgg acctggcggc cttcacgctg
    34621 gctcgtgacc ataaattacc gattcgtgtt ttcaatatga acaaaccggg tgcgctgcgc
    34681 cgtgtggtaa tgggtgaaaa agaagggact ttaatcacgg aataattccc gtgatggata
    34741 aataagggta agattccgcg taagtatcgc gggggcgtaa gtctggttat aaggcgttat
    34801 tgttgcaggc agtttggtca cggccagcgc gcagcaaccg gagcgtacaa aagtacgtga
    34861 ggatggcgag cactgctcgg ggccaaaatg gcaaataaaa tagcctaata atccagacga
    34921 ttacccgtaa tatgtttaat cagggctata cttagcacac ttccactgtg tgtgactgtc
    34981 tggtctgact gagacaagtt ttcaaggatt cgtaacgtga ttagcgatat cagaaaagat
    35041 gctgaagtac gcatggacaa atgcgtagaa gcgttcaaaa cccaaatcag caaaatacgc
    35101 acgggtcgtg cttctcccag cctgctggat ggcattgtcg tggaatatta cggcacgccg
    35161 acgccgctgc gtcagctggc aagcgtaacg gtagaagatt cccgtactct gaaaatcaac
    35221 gtgtttgatc gttcaatgtc tccggccgtt gaaaaagcga ttatggcctc cgatctcggc
    35281 ctgaacccga actctgcggg tagcgacatc cgtgttccgc tgccgccgct gacggaagaa
    35341 cgtcgtaaag atctgaccaa aatcgttcgt ggtgaagcag aacaagcgcg tgttgcagta
    35401 cgtaatgtgc gtcgtgacgc gaacgacaaa gtgaaagcac tgttgaaaga taaagagatc
    35461 agcgaagacg acgatcgccg ttctcaggac gatgtacaga aactgactga tgctgcaatc
    35521 aagaaaattg aagcggcgct ggcagacaaa gaagcagaac tgatgcagtt ctgatttctt
    35581 gaacgacaaa aacgccgctc agtagatcct tgcggatcgg ctggcggcgt tttgcttttt
    35641 attctgtctc aactctggat gtttcatgaa gcaactcacc attctgggct cgaccggctc
    35701 gattggttgc agcacgctgg acgtggtgcg ccataatccc gaacacttcc gcgtagttgc
    35761 gctggtggca ggcaaaaatg tcactcgcat ggtagaacag tgcctggaat tctctccccg
    35821 ctatgccgta atggacgatg aagcgagtgc gaaacttctt aaaacgatgc tacagcaaca
    35881 ggggagccgc accgaagtct taagtgggca acaagccgct tgcgatatgg cagcgcttga
    35941 ggaggttgat caggtgatgg cagccattgt tggtgctgct gggctgttac ctacgcttgc
    36001 tgcgatccgc gcgggtaaaa ccattttgct ggccaataaa gaatcactgg ttacctgcgg
    36061 acgtctgttt atggacgccg taaagcagag caaagcgcaa ttgttaccgg tcgatagcga
    36121 acataacgct atttttcaga gtttaccgca acctatccag cataatctgg gatacgctga
    36181 ccttgagcaa aatggcgtgg tgtccatttt acttaccggg tctggtggcc ctttccgtga
    36241 gacgccattg cgcgatttgg caacaatgac gcccgatcaa gcttgccgtc atccgaactg
    36301 gtcgatggga cgtaaaattt ctgtcgattc ggctaccatg atgaacaaag gtctggaata
    36361 cattgaagcg cgttggctgt ttaacgccag cgccagccag atggaagtgc tgattcaccc
    36421 gcagtcagtg attcactcaa tggtgcgcta tcaggacggc agtgttctgg cgcagctggg
    36481 ggaaccggat atgcgtacgc caattgccca caccatggca tggccgaatc gcgtgaactc
    36541 tggcgtgaag ccgctcgatt tttgcaaact aagtgcgttg acatttgccg caccggatta
    36601 tgagcgttat ccatgcctga aactggcgat ggaggcgttc gaacaaggcc aggcagcgac
    36661 gacagcattg aatgccgcaa acgaaatcac cgttgctgct tttcttgcgc aacaaatccg
    36721 ctttacggat atcgctgcgt tgaatttatc cgtactggaa aaaatggata tgcgcgaacc
    36781 acaatgtgtg gacgatgtgt tatctgttga tgcgaacgcg agtgaagtcg ccagaaaaga
    36841 ggtgatgcgt ctcgcaagct gaggataatc cggctacaga gagtcgcgct atttgttagc
    36901 gtagggcttc agtgatatag tctgcgccat ctgatcgtaa gtagttggct ttataaggtc
    36961 aggtatgccg tggttttaca cggctttttt ttgtataggc ttcagtattc ctgagtaccg
    37021 taaaccctgt cagggaataa aaaacgcgtg atgttgtctg ctactcaacc acttagcgaa
    37081 aaattgccag cgcatggctg ccgtcatgtt gcgatcatta tggacggcaa tggtcgctgg
    37141 gcaaaaaagc aagggaagat tcgtgccttt gggcataaag ccggggcaaa atccgtccgc
    37201 cgggctgtct cttttgcggc caacaacggt attgaggcgt taacgctgta tgcctttagt
    37261 agtgaaaact ggaaccgacc agcgcaggaa gtcagtgcgt taatggaact gtttgtgtgg
    37321 gcgctcgata gcgaagtaaa aagtctgcac cgacataacg tgcgtctgcg tattattggc
    37381 gataccagtc gttttaactc gcgtttgcaa gaacgtattc gtaaatctga agcgctaaca
    37441 gccggaaata ccggtctgac gctgaatatt gcggcgaact acggtggacg ttgggatata
    37501 gtccagggag tcaggcaact ggctgaaaag gtgcagcaag gaaacctgca accagatcag
    37561 atagatgaag agatgttaaa ccagcatgtc tgtatgcatg aactggcccc tgtagattta
    37621 gtaattagga ctgggggaga gcatcgcatt agtaactttt tgctttggca aattgcctat
    37681 gccgaacttt actttacaga tgttctctgg cccgatttcg atgaacaaga ctttgaaggg
    37741 gcgttaaatg cctttgctaa tcgagagcgt cgtttcggcg gcaccgagcc cggtgatgaa
    37801 acagcctgat gggggtcgct tttgctgaag tatcgcctga tatctgcttt tgtgttaata
    37861 cccgtcgtca tcgcggcgtt gtttctgttg ccgccggtgg ggttcgccat tgtaacgctg
    37921 gtggtctgca tgctggcagc gtgggaatgg ggacagctta gcggttttac cactcgttcg
    37981 cagcgagtat ggttggcggt gttatgcggg ttattgttgg cgctgatgct ttttctgttg
    38041 ccggaatatc accgaaatat tcatcaaccg ctggttgaaa tctcactttg ggcttcgttg
    38101 ggttggtgga ttgtcgcgct attgctggtg ctgttttacc caggttccgc agcaatctgg
    38161 cgtaactcta aaacattgcg ccttattttt ggcgtgctaa ccattgttcc cttcttctgg
    38221 ggcatgctgg cgttacgggc ctggcactat gacgagaatc attacagtgg cgcaatatgg
    38281 ctgctctatg tcatgatcct ggtatggggc gctgactccg gcgcatatat gtttggcaaa
    38341 ttgtttggta aacataagct ggcaccgaag gtttctccgg gtaaaacctg gcaaggcttt
    38401 ataggtggac tcgctactgc agcggtaatc tcatggggtt atggcatgtg ggcgaatctc
    38461 gacgtcgctc ccgtcacctt actcatttgc tctattgtcg cagcgttagc ctcagtgctc
    38521 ggcgatctga ccgagagtat gtttaagcgt gaagcaggaa ttaaggacag cggtcattta
    38581 attccaggac acggtggtat tttagatcgt attgatagcc tgacggctgc ggtaccggtc
    38641 tttgcttgct tgttgttact ggtattcagg acgctttaac ggaaggtaat atgctgagtt
    38701 ttctctggga tttggcttcg ttcatcgttg cactgggtgt acttatcacc gtgcatgaat
    38761 ttggtcattt ctgggttgcc cggcgttgtg gtgttcgcgt tgagcgtttc tcaatagggt
    38821 ttggtaaggc gctctggcgg cgaactgata agctcggcac cgaatatgtt atcgccctga
    38881 tcccgttggg cggttatgtc aaaatgctgg atgagcgcgc agaaccggtc gttccggaac
    38941 tccgccacca tgccttcaat aataaatctg tcggccaacg agcggcgatt attgccgcag
    39001 gtccggttgc aaacttcatt tttgctatct ttgcctactg gctggttttt attattggtg
    39061 tgcctggcgt acgtccggtg gttggcgaaa tagcagccaa ttcgatagct gcggaagcac
    39121 aaattgcacc aggtacggaa ctaaaagccg tagatggtat cgaaacgcct gattgggatg
    39181 ccgtgcgttt gcagttggtc gataaaattg gcgatgaaag caccaccatt acagtagcgc
    39241 catttggcag cgaccaacgg cgggatgtaa agctcgattt acgtcactgg gcgtttgagc
    39301 ctgataaaga agatccggta tcttcgctgg ggattcgtcc tcgtgggccg caaattgaac
    39361 ctgtactgga aaatgtgcag ccaaactcgg cggcaagcaa ggcaggtttg caagcaggcg
    39421 acaggatcgt taaagtcgat ggtcagccct taacgcagtg ggtgaccttt gtgatgcttg
    39481 tccgggataa cccgggtaaa tccttagcgt tagaaatcga aaggcagggg agtcccttgt
    39541 ctttgacatt aatcccgggg agtaaaccgg gtaatggtaa agcgattggt tttgtcggta
    39601 ttgagccgaa agtcattcct ttgccagatg agtataaagt tgtacgccag tatgggccgt
    39661 tcaacgccat cgtcgaagcc acggacaaaa cgtggcagct gatgaagctg acggtcagta
    39721 tgctgggaaa attgatcacc ggtgatgtga aactgaacaa cctcagtggg ccgatctcta
    39781 tcgccaaggg ggctgggatg acagcggaac tcggggtagt ttattacctg ccgtttcttg
    39841 cgcttattag cgtgaactta gggataatta acctgtttcc gttgcccgta cttgacgggg
    39901 ggcatctgct gttccttgcg atcgaaaaga tcaagggcgg accggtatcc gagcgggttc
    39961 aagacttttg ttatcgcatt ggctcgattc tgctggtgct gttaatgggg cttgcacttt
    40021 tcaatgattt ctctcggtta tgagagttag ttaggaagaa cgcataataa cgatggcgat
    40081 gaaaaagttg ctcatagcgt cgctgctgtt tagcagcgcc accgtatacg gtgctgaagg
    40141 gttcgtagtg aaagatattc atttcgaagg ccttcagcgt gtcgccgttg gtgcggccct
    40201 cctcagtatg ccggtgcgta caggcgacac ggttaatgat gaagatatca gtaataccat
    40261 tcgcgctctg tttgctaccg gcaactttga ggatgttcgc gtccttcgtg atggtgatac
    40321 ccttctggtt caggtaaaag aacgtccgac cattgccagc attactttct ccggtaacaa
    40381 atcggtgaaa gatgacatgc tgaagcaaaa cctcgaggct tctggtgtgc gtgtgggcga
    40441 atccctcgat cgcaccacca ttgccgatat cgagaaaggt ctggaagact tctactacag
    40501 cgtcggtaaa tatagcgcca gcgttaaagc tgtcgtgacc ccgctgccgc gcaaccgtgt
    40561 tgacctaaaa ctggtgttcc aggaaggtgt gtcagctgaa atccagcaaa ttaacattgt
    40621 tggtaaccat gctttcacca ccgacgaact gatctctcat ttccaactgc gtgacgaagt
    40681 gccgtggtgg aatgtggtag gcgatcgtaa ataccagaaa cagaaactgg cgggcgatct
    40741 tgaaaccctg cgcagctact atctggaccg cggttatgcc cgtttcaaca tcgactctac
    40801 ccaggtcagt ctgacgccag ataaaaaagg tatttacgtc acggtgaaca tcaccgaagg
    40861 cgatcagtac aagctttctg gcgttgaagt gagcggcaac cttgccgggc actccgctga
    40921 aattgagcag ctgactaaga tcgagccggg cgagctgtat aacggcacta aagtgaccaa
    40981 aatggaagat gacattaaaa agcttctcgg tcgctatggt tatgcctatc cgcgcgtaca
    41041 gtcgatgcct gaaattaacg atgccgacaa aaccgttaaa ttacgcgtga acgttgatgt
    41101 gggtaaccgt ttctacgtgc gtaagatccg ttttgaaggt aacgatacct cgaaagatgc
    41161 cgtcctgcgt cgcgaaatgc gtcagatgga aggtgcatgg ttggggagcg atctggtcga
    41221 tcagggtaag gagcgtctga atcgtctggg cttctttgaa actgtcgata ccgataccca
    41281 acgtgttccg ggtagcccgg accaggttga tgtagtctac aaggtgaaag aacgtaacac
    41341 tggtagcttc aacttcggga ttggttacgg tactgaaagt ggcgtgagct tccaggctgg
    41401 cgtgcaacag gataactggt taggtacagg ttatgctgtt ggtatcaacg ggaccaaaaa
    41461 cgattaccag acctatgctg aactgtcggt aaccaacccg tacttcaccg tagatggcgt
    41521 aagcctcggt ggtcgtctct tctataatga cttccaggca gatgacgccg acctgtccga
    41581 ctataccaac aagagttatg gtacagacgt gacgttgggc ttcccgatta acgaatataa
    41641 ctcgctgcgt gcaggtctgg gttatgtaca taactccctg tccaacatgc agcctcaggt
    41701 tgcgatgtgg cgttatctgt actctatggg tgaacatccg agcacctctg atcaggataa
    41761 cagcttcaaa acggacgact tcacgttcaa ctatggttgg acctataaca agcttgaccg
    41821 tggttacttc ccgacagatg gttcacgtgt caacctgacc ggtaaagtga ccattcctgg
    41881 atcggataac gaatactaca aagtgacgtt agacacggcg acttatgtgc cgatcgatga
    41941 cgatcacaaa tgggttgttc tgggtcgtac ccgctggggt tatggtgatg gtttaggcgg
    42001 caaagagatg ccgttctacg agaacttcta tgccggtggt tccagcaccg tgcgtggctt
    42061 ccagtccaat accattggtc cgaaagcagt ttacttcccg catcaggcca gtaattatga
    42121 tccggactat gattacgaat gtgcgactca ggacggcgcg aaagacctgt gtaaatcgga
    42181 tgatgctgta ggcggtaacg ccatggcggt tgccagcctc gagttcatca ccccgacgcc
    42241 gtttattagc gataagtatg ctaactcggt tcgtacttca ttcttctggg atatgggtac
    42301 cgtttgggat acaaactggg attccagcca atattctgga tatccggact atagtgatcc
    42361 aagcaatatc cgtatgtctg cgggtatcgc attacaatgg atgtccccat tggggccgtt
    42421 ggtgttctcc tacgcccagc cgttcaaaaa gtacgatgga gacaaggcag aacagttcca
    42481 gtttaacatc ggtaaaacct ggtaagtgtt ctccacaaag gaatgtagtg gtagtgtagc
    42541 gatgacttta ggcgatcaat ataagatcgc cgggccacgc aaagaactgc accctccggt
    42601 gcaaatggga tggtaaggag tttattgtga aaaagtggtt attagctgca ggtcttggtt
    42661 tagcactggc aacttctgct caggcggctg acaaaattgc aatcgtcaat atgggcagcc
    42721 tgttccagca ggtagcgcag aaaaccggtg tttctaacac gctggaaaat gagttcaaag
    42781 gccgtgccag cgaactgcag cgtatggaaa ccgatctgca ggctaaaatg aaaaaactgc
    42841 agtccatgaa agcgggcagc gatcgcacca aactggaaaa agacgtgatg gctcagcgcc
    42901 agacttttgc tcagaaagcg caggcttttg agcaggatcg cgcacgtcgt tccaacgaag
    42961 aacgcggcaa actggttact cgtatccaga ctgctgtgaa atccgttgcc aacagccagg
    43021 atatcgatct ggttgttgat gcaaacgccg ttgcttacaa cagcagcgat gtaaaagaca
    43081 tcactgccga cgtactgaaa caggttaaat aagtaatgcc ttcaattcga ctggctgatt
    43141 tagcgcagca gttggatgca gaactacacg gtgatggcga tatcgtcatc accggcgttg
    43201 cgtccatgca atctgcacaa acaggtcaca ttacgttcat ggttaaccca aaataccgtg
    43261 agcatttagg cttgtgccag gcgtccgcgg ttgtcatgac ccaggacgat cttcctttcg
    43321 cgaaaagtgc cgcgctggta gtgaagaatc cctacctgac ttacgcgcgc atggcgcaaa
    43381 ttttagatac cacgccgcag cccgcgcaga acattgcacc cagtgcggtg atcgacgcga
    43441 cggcgaagct gggtaacaac gtatcgattg gcgctaacgc ggtgattgag tccggcgttg
    43501 aactgggcga taacgtgatt atcggtgccg gttgcttcgt aggtaaaaac agcaaaatcg
    43561 gtgcaggttc gcgtctctgg gcgaacgtaa ccatttacca tgagatccag atcggtcaga
    43621 attgcctgat ccagtccgga acagtggtag gcgcagacgg ctttggttat gccaacgatc
    43681 gtggtaactg ggtgaagatc ccacagattg gtcgcgtaat tattggcgat cgcgtggaga
    43741 tcggtgcctg cacaaccatc gatcgcggcg cgctggatga cactgttatt ggcaatggcg
    43801 tgatcattga taaccagtgc cagattgcac ataacgtcgt gattggcgac aatacggcgg
    43861 ttgccggtgg cgtcattatg gcgggcagcc tgaaaattgg tcgttactgc atgatcggcg
    43921 gagccagcgt aatcaacggg catatggaaa tatgcgacaa agtgacggtt acgggcatgg
    43981 gtatggtgat gcgtcccatc actgaaccag gcgtctattc ctcaggcatt ccgctgcaac
    44041 ccaacaaagt ctggcgcaaa accgctgcac tggtgatgaa cattgatgac atgagcaagc
    44101 gtctgaaatc gcttgagcgc aaggttaatc aacaagacta acgttccatc ttttgttcgc
    44161 caaactttac ggcctgtctc attcttacga ttgcggcagg ccgtgttatt attgtcgttt
    44221 cttatatttt gacaggaaga gtatcttgac tactaacact catactctgc agattgaaga
    44281 gattttagaa cttctgccgc accgtttccc gttcttactg gtggatcgcg tgctggattt
    44341 tgaagaaggt cgttttctgc gcgcagtaaa aaatgtctct gtcaatgagc cattcttcca
    44401 gggccatttc cctggaaaac cgattttccc gggtgtgctg attctggaag caatggcaca
    44461 ggcaacaggt attctggcgt ttaaaagcgt aggaaaactg gaaccgggtg agctgtacta
    44521 cttcgctggt attgacgaag cgcgcttcaa gcgcccggtc gtgcctggcg atcaaatgat
    44581 catggaagtc actttcgaaa aaacgcgccg cggcctgacc cgttttaaag gggttgctct
    44641 ggtcgatggt aaagtagttt gcgaagcaac gatgatgtgt gctcgtagcc gggaggcctg
    44701 atacgtgatt gataaatccg cctttgtgca tccaaccgcc attgtggaag agggcgcgtc
    44761 aattggcgcg aacgcacaca ttggtccttt ttgtatcgtt ggaccccatg tcgaaattgg
    44821 tgagggtacc gtactgaaat ctcacgttgt cgtgaatggt catactaaaa ttggccgcga
    44881 taatgagatt tatcagtttg cctccatcgg cgaagttaac caggatttga aatatgctgg
    44941 cgaaccgacc cgtgtggaaa tcggcgatcg taaccgcatt cgcgaaagcg tcaccattca
    45001 tcgtggcaca gtccagggcg gtggattgac gaaggtgggc agcgacaact tactgatgat
    45061 caacgcgcac attgcgcacg attgtacggt aggtaaccgc tgtattctcg ccaacaacgc
    45121 aacgctggcg ggtcacgtat cggttgacga cttcgcgatc atcggcggca tgaccgcagt
    45181 ccatcagttc tgcatcattg gtgcgcatgt gatggttggc ggctgctccg gtgtggcgca
    45241 ggacgtccct ccttatgtca ttgcgcaggg taaccacgca acgccgttcg gtgtcaatat
    45301 cgaagggctg aagcgccgcg gattcagccg tgaggcgatt accgctatcc gcaatgcgta
    45361 taagctaatt tatcgtagcg gtaaaacgct cgatgaagtg aaaccggaaa ttgctgaact
    45421 ggcggaaaca tatccggaag tgaaagcctt taccgatttc tttgcacgct caacgcgcgg
    45481 tctgattcgt taatgactga ccagcgtcca ttaacgattg ccctggtcgc cggagaaacc
    45541 tccggcgata tcctgggggc cggtttaatc cgcgctctga aagaacgtgt gcccaacgcc
    45601 cgctttgttg gtgttgccgg gccacgaatg caggctgaag gctgcgaagc ctggtacgaa
    45661 atggaagaac tggcggtgat gggcattgtt gaagtgctcg gtcgtctgcg tcgcttactg
    45721 catattcgtg ccgatctgac aaagcgtttt ggcgaactga agccagatgt ttttgttggt
    45781 attgatgcgc ccgacttcaa tattactctt gaaggtaacc tcaaaaagca gggtatcaaa
    45841 accattcatt acgtcagtcc atccgtctgg gcgtggcgac agaaacgcgt tttcaaaata
    45901 ggcagagcca ccgatctggt gctcgcattt ctgcctttcg aaaaagcgtt ttatgacaaa
    45961 tacaatgtac cgtgccgctt tatcggtcat accatggctg atgccatgcc gttagatcca
    46021 gataaaaatg gtgcccgtga tgtgctgggg atcccttacg atgcccactg tctggcattg
    46081 ttgccgggca gccgtggtgc agaagtcgaa atgcttagtg ccgatttctt gaaaactgcc
    46141 cagcttttgc gccagacata tccggatctc gaaatcgtgg tgccgctggt gaatgccaaa
    46201 cgccgcgagc agtttgaacg catcaaagct gaagtcgcgc cagacctgtc ggttcatttg
    46261 ctggatggaa tgggccgtga ggcgatggtc gccagcgatg cggcactact ggcgtcgggg
    46321 acggcagccc tggagtgtat gctggcgaaa tgcccgatgg tggtgggata tcgcatgaag
    46381 ccttttacct tctggttggc gaagcggctg gtgaaaactg attatgtctc gctgccaaat
    46441 ctgctggcgg gcagagagtt agtcaaagag ttattgcagg aagagtgtga gccgcaaaaa
    46501 ctggctgcgg cgctgttacc gctgttggcg aacgggaaaa ccagccacgc gatgcacgat
    46561 accttccgtg aactgcatca gcagatccgc tgcaatgccg atgagcaggc ggcacaagcc
    46621 gttctggagt tagcacaatg atcgaatttg tttatccgca cacgcagctg gttgcgggtg
    46681 tggatgaagt cggacgcggg ccgttagttg gcgcggtcgt caccgctgcg gtgatccttg
    46741 acccggcgcg cccgattgcc gggctgaatg attccaaaaa gctgagcgaa aaacgccgtc
    46801 tggcgctcta tgaagagatc aaagagaaag cgttgagctg gagtctgggc cgcgcggaac
    46861 cccacgaaat cgacgagctg aacattcttc atgcgaccat gctggcgatg cagcgtgccg
    46921 tcgctgggct gcatattgcg ccggaatatg tgttgattga tggtaaccgc tgcccgaaat
    46981 taccgatgcc tgcgatggct gtggtgaaag gggatagccg cgtaccagaa atcagtgccg
    47041 cgtctatcct ggcgaaagtg acgcgtgacg ccgaaatggc ggcgctggat attgttttcc
    47101 cgcaatatgg ttttgcccaa cataaagggt atccaaccgc ttttcatctg gaaaaactgg
    47161 ctgaatatgg cgcgactgaa catcatcgtc gcagctttgg gcctgtcaaa cgcgcactgg
    47221 gacttgcgtc ctgattcttg tgtcgagatt aagtaaaccg gaatctgaag atgtctgaac
    47281 cacgtttcgt acacctgcgg gtgcacagcg actactcgat gatcgatggc ttggccaaaa
    47341 ccgcaccgct ggtaaaaaag gcggcggcgt tgggtatgcc agcactggcg atcaccgatt
    47401 tcaccaacct ttgcggtctg gtgaagttct acggagcggg acatggcgca gggattaagc
    47461 ctatcgtcgg ggcagatttt aatgtccagt gcgacctgct gggtgatgag ttaacccacc
    47521 tgacggtact ggcggcgaac aataccggct atcagaatct ggcgttgctg atctcaaaag
    47581 cgtatcagcg cgggtacggt gccgccgggc cgatcatcga tcgcgactgg cttatcgaat
    47641 taaacgaagg gttgatcctt ctttccggcg ggcgcatggg cgacgtcgga cgcagtcttt
    47701 tgcgtggtaa cagcgcgctg gtagatgagt gtgtcgcgtt ttatgaagaa cacttcccgg
    47761 atcgctattt tctcgagctg atccgcaccg gcaggccgga tgaagaaagc tatctgcacg
    47821 cggcggtgga actggcggaa gcgcgcggtt tgcccgtcgt ggcgactaac gatgtgcgct
    47881 ttatcgacag cagcgacttt gacgcacacg aaatccgcgt cgcgatccac gacggcttta
    47941 ccctcgacga tcctaaacgc ccgcgtaact attcgccgca gcaatatatg cgtagcgaag
    48001 aggagatgtg tgagctgttt gccgacatcc cagaagccct tgccaacacc gttgagatcg
    48061 ccaaacgctg taacgtaact atgcgtcttg gtgaatactt cctgccgcag ttcccgaccg
    48121 gggacatgag caccgaagat tatctggtca agcgtgcaaa agaaggcctg gaagagcgtc
    48181 tggcattttt attccctgac gaggaagaac gtcttaaacg ccgcccggaa tatgacgaac
    48241 gtctggagac tgaacttcag gttatcaacc agatgggctt cccgggctac ttcctcatcg
    48301 ttatggaatt tatccagtgg tcgaaagata acggcgtacc ggtagggcca ggccgtggct
    48361 ccggtgcggg ttcactggtg gcctacgcgc tgaaaatcac cgacctcgat ccgctggaat
    48421 ttgacctgct gttcgaacgt ttccttaacc cggaacgtgt ctccatgcct gacttcgacg
    48481 ttgacttctg tatggagaaa cgcgatcagg ttatcgaaca cgtggcggac atgtacggtc
    48541 gtgatgcggt atcgcagatt atcaccttcg gtacgatggc ggcgaaagcg gtgatccgcg
    48601 acgtaggccg cgtgctgggg catccgtacg gctttgtcga ccgtatctcg aaactgatcc
    48661 cgcccgatcc ggggatgacg ctggcgaaag cgtttgaagc cgagccgcag ctgccggaaa
    48721 tctacgaagc ggatgaagaa gttaaggcgc tgatcgacat ggcgcgcaaa ctggaagggg
    48781 tcacccgtaa cgccggtaag cacgccggtg gggtggttat cgcgccgacc aaaattaccg
    48841 attttgcgcc gctttattgc gatgaagagg gcaaacatcc ggtcacccag tttgataaaa
    48901 gcgacgttga atacgccgga ctggtgaagt tcgacttcct tggtttgcgt acgctcacca
    48961 tcatcaactg ggcgctggag atgatcaaca agcggcgggc gaagaatggc gagccaccgc
    49021 tggatatcgc cgcgatcccg ctggatgaca agaaaagctt cgacatgctg caacgctcgg
    49081 aaaccacggc ggtattccag cttgaatcgc gcggcatgaa ggacctgatc aagcgtctgc
    49141 aacctgactg cttcgaagat atgatcgcac tggtggcact gttccgccct ggtccgttgc
    49201 agtcagggat ggtggataac tttatcgacc gtaaacatgg tcgtgaagag atctcctatc
    49261 cggacgtgca gtggcagcat gaaagcctga aatcggtact ggagccaacc tacggcatta
    49321 tcttgtatca ggaacaggtc atgcagattg cccaggtgct ttctggttat accctcggtg
    49381 gcgcggatat gctgcgccgt gcgatgggta agaaaaagcc ggaagagatg gctaagcagc
    49441 gttctgtatt tgctgaaggt gcagaaaaga acggaatcaa cgccgaactg gcgatgaaaa
    49501 tcttcgacct ggtggagaaa ttcgcgggtt acggatttaa caaatcgcac tctgcggcct
    49561 atgctttggt gtcatatcaa acgttatggc tgaaagcgca ctatccggcg gagtttatgg
    49621 cggcggtaat gaccgccgat atggacaaca ccgagaaggt ggtgggcctg gtggatgagt
    49681 gctggcggat ggggctgaaa atcctgccac cagatataaa ctccggtctt taccatttcc
    49741 acgtcaacga cgacggcgaa atcgtgtatg gtattggcgc catcaaaggg gtaggtgaag
    49801 gtccgattga ggccatcatc gaagcccgta ataaaggcgg ctacttccgc gaactgtttg
    49861 atctctgcgc ccgtaccgac accaaaaagt taaaccggcg ggtgctggaa aaactgatca
    49921 tgtccggggc gtttgaccgt cttgggccac accgcgcggc gctgatgaac tcgctgggtg
    49981 atgcgttaaa agcggcagat caacacgcga aagcggaagc tatcggtcag gccgatatgt
    50041 tcggcgtgct ggccgaagag ccggaacaaa ttgaacaatc ctacgccagc tgccaaccgt
    50101 ggccggagca ggtggtatta gatggggaac gtgaaacgtt aggcctgtac ctgaccggac
    50161 accctatcaa ccagtattta aaagagattg agcgttatgt cggaggcgta aggctgaaag
    50221 acatgcaccc gacagaacgt ggtaaagtca tcacggctgc ggggctcgtt gttgccgcgc
    50281 gggttatggt caccaagcgc ggcaatcgta tcggtatctg cacgctggat gaccgttccg
    50341 ggcggctgga agtgatgttg tttactgacg ccctggataa ataccagcaa ttgctggaaa
    50401 aagaccgcat acttatcgtc agcggacagg tcagctttga tgacttcagc ggtgggctta
    50461 aaatgaccgc tcgcgaagtg atggatattg acgaagcccg ggaaaaatat gctcgcgggc
    50521 ttgctatctc gctgacggac aggcaaattg atgaccagct tttaaaccga ctccgtcagt
    50581 ctctggaacc ccaccgctct gggacaattc cagtacatct ctactatcag agggcggatg
    50641 cacgcgcgcg gttgcgtttt ggcgcgacgt ggcgtgtctc tccgagcgat cgtttattaa
    50701 acgatctccg tggcctcatt ggttcggagc aggtggaact ggagtttgac taatacagga
    50761 atactatgag tctgaatttc cttgattttg aacagccgat tgcagagctg gaagcgaaaa
    50821 tcgattctct gactgcggtt agccgtcagg atgagaaact ggatattaac atcgatgaag
    50881 aagtgcatcg tctgcgtgaa aaaagcgtag aactgacgcg taaaatcttc gccgatctcg
    50941 gtgcatggca gattgcgcaa ctggcacgcc atccgcagcg tccttatacc ctggattacg
    51001 ttcgcctggc atttgatgaa tttgacgaac tggctggcga ccgtgcgtat gcagacgata
    51061 aagctatcgt cggtggtatc gcccgtctcg atggtcgtcc ggtgatgatc attggtcatc
    51121 aaaaaggtcg tgaaaccaaa gaaaaaattc gccgtaactt tggtatgcca gcgccagaag
    51181 gttaccgcaa agcactgcgt ctgatgcaaa tggctgaacg ctttaagatg cctatcatca
    51241 cctttatcga caccccgggg gcttatcctg gcgtgggcgc agaagagcgt ggtcagtctg
    51301 aagccattgc acgcaacctg cgtgaaatgt ctcgccttgg cgtaccggta gtttgtacgg
    51361 ttatcggtga aggtggttct ggcggtgcgc tggcgattgg cgtgggcgat aaagtgaata
    51421 tgctgcaata cagcacctat tccgttatct cgccggaagg ttgtgcgtcc attctgtgga
    51481 agagcgccga caaagcgccg ctggcggctg aagcgatggg tatcatcgct ccgcgtctga
    51541 aagagctgaa actgatcgac tccatcatcc cggaaccgct gggcggcgct caccgtaacc
    51601 cggaagcgat ggcggcatcg ttgaaagcgc aactgctggc ggatctggcc gatctcgatg
    51661 tgttaagcac tgaagattta aaaaatcgtc gttatcagcg cctgatgagc tacggttacg
    51721 cgtaattcgc aaaagttctg aaaaagggtc acttcggtgg ccctttttta tcgccacggt
    51781 ttgagcaggc tatgattaag gaaggatttt ccaggaggaa cacatgaaca tcattgccat
    51841 tatgggaccg catggcgtct tttataaaga tgagcccatc aaagaactgg agtcggcgct
    51901 ggtggcgcaa ggctttcaga ttatctggcc acaaaacagc gttgatttgc tgaaatttat
    51961 cgaacataac cctcgaattt gcggcgtgat ttttgactgg gatgagtaca gtctcgattt
    52021 atgcagcgat atcaatcaac ttaatgaata tctcccgctt tatgccttca tcaacaccca
    52081 ctcgacgatg gatgtcagcg tgcaggatat gcggatggcg ctctggtttt ttgaatatgc
    52141 gctggggcag gcggaagata tcgccattcg tatgcgtcag tacaccaacg aatatcttga
    52201 taacattacg ccgccgttca cgaaagcctt gtttacctac gtcaaagagc ggaagtacac
    52261 cttttgtacg ccggggcata tgggcggcac cgcatatcaa aaaagcccgg ttggctgtct
    52321 gttttatgat tttttcggcg ggaataccct taaggctgat gtctctattt cggtcaccga
    52381 gcttggttcg ttgcttgacc acaccgggcc acatctggaa gcggaagagt acatcgcgcg
    52441 gacttttggc gcggaacaga gttatatcgt taccaacgga acatcgacgt cgaacaaaat
    52501 tgtgggtatg tacgccgcgc catccggcag tacgctgttg atcgaccgca actgccataa
    52561 atcactggcg catctgttga tgatgaacga tgtagtgcca gtctggctga aaccgacgcg
    52621 taatgcgttg gggattctgg gggggatccc gcgccgtgaa tttactcgcg acagcatcga
    52681 agagaaagtc gctgccacta cgcaagcaca atggccggtt catgcggtga tcaccaactc
    52741 cacctatgat ggtttgctct acaacaccga ctggatcaaa cagacgctgg atgtcccgtc
    52801 gatccacttc gattccgcct gggtgccgta tactcatttt catccaatct accagggtaa
    52861 aagtggtatg agcggcgagc gtgttgcggg aaaagttttc ttcgaaacgc aatcgaccca
    52921 caaaatgctg gcggcgttat cgcaggcttc actgatccac attaaaggcg agtatgacga
    52981 agaggcgttt aacgaagcct ttatgatgca taccaccacc tcgcccagtt atcccattgt
    53041 tgcttcggtt gagacggcgg cggcgatgct gcgtggtaat ccgggcaaac ggctgattaa
    53101 ccgttcggtg gagcgggcgc tgcattttcg caaagaggtc cagcggctgc gggaagagtc
    53161 tgacggttgg tttttcgata tctggcaacc gccgcaggtg gatgaagccg aatgcaggcc
    53221 cgttgcgcct ggcgaacaat ggcacggctt taacgatgcg gatgccgatc atatgtttct
    53281 cgatccggtt aaagtcacta ttttgacacc ggggatggac gaacagggca atatgagtga
    53341 ggaggggatc ccggcggcgc tggtagcaaa attcctcgac gaacgtggga tcgtagtaga
    53401 gaaaaccggc ccttataacc tgctgtttct ctttagtatt ggcatcgata aaaccaaagc
    53461 aatgggatta ttgcgtgggt tgacggaatt taagcgctct tacgatctca acctgcggat
    53521 caaaaatatg ctgcccgatc tctatgctga agatcccgat ttctaccaca atatgcgtat
    53581 tcaggatctg gcgcaaggga tccataagct gattcgtaaa cacgatcttt ccggtttgat
    53641 gttgcgggca ttcgatactt tgccggaaat gatcatgacg ccacatcagg catggcagag
    53701 acagattaaa ggtgaagtag aaaccattgc gctggaacaa ctggtcggta gagtatcggc
    53761 aaatatgatc ctgccttatc caccgggcgt accgctgttg atgccaggag aaatgctgac
    53821 caaagagagc cgcacggtac tcgattttct actgatgctt tgttccgtag ggcaacatta
    53881 ccccggtttt gaaacggata ttcacggcgc gaaacaggac gaagacggcg tttaccgcgt
    53941 acgagtccta aaaatggcgg gataacttgc cagagcggct tccgggcgag taacgtgctg
    54001 ttaacaaata aaggagacgt tatgctgggt ttaaaacagg ttcaccatat tgcgattatt
    54061 gcgacggatt atgcggtgag caaagctttc tactgcgata ttcttggttt cacgctgcaa
    54121 agcgaagtct atcgcgaagc gcgcgactcg tggaaagggg atttggcgct taatgggcaa
    54181 tatgtgattg agcttttctc ttttccgttc ccgccggagc gccccagccg accggaagct
    54241 tgcggcctgc gtcatctggc ttttagcgtt gatgacatcg atgcggcagt ggcgcacctt
    54301 gaaagccata acgtgaagtg tgaagccatc cgtgtcgatc catacacgca aaaacgcttc
    54361 accttcttta acgatccgga cgggctgccg ttggaactgt atgagcagta aggcttgtca
    54421 tcgccgcatt tgcccggtaa cgtgccgggc attgctactg taaaatcgca ccatcatgac
    54481 actcacgctc aatagacaac ttctcacctc acgccagatt ctggtggcct ttagcggcgg
    54541 gcttgactcc accgttctgc tgcatcagtt ggtgcagtgg cggacggaaa atccgggcgt
    54601 cactctgcgc gctattcatg tgcatcacgg tttaagtgcc aatgccgatg cctgggttac
    54661 gcattgcgaa aacgtctgcc aacagtggca ggtgccgctg gtggtcgaac gcgtacaact
    54721 tgcgcaagag gggctgggga ttgaggccca ggcgcggcag gcacgttatc aggcatttgc
    54781 ccgtacccta ttgcccggcg aagtgctggt caccgcgcaa catctcgacg atcaatgtga
    54841 aacctttctg ctggcgctaa aacgcggtag cggccctgcc gggctttcgg ctatggcgga
    54901 agtttcggag tttgctggaa cacggcttat ccgcccgttg cttgcccgca cgcgggggga
    54961 actggagcag tgggcattgg cgcatggttt acgctggatt gaagacgaaa gtaatcagga
    55021 cgactcatac gatcgtaact ttctgcgcct gcgcgtagtg ccgttattgc agcagcgttg
    55081 gccgcatttt gcggaagcaa cggcccgcag cgccacactt tgtgctgaac aagagagtct
    55141 gttggatgaa ctgctggcgg atgatttagc acactgccaa acgtcgcagg ggacgctgca
    55201 gattgcgcca atgctggcga tgagtgatgc ccgtcgcgcg gcgattattc gccgctggct
    55261 ggcagggcag aatgcaccga tgccttcccg cgacgcgctg gtgcgcatct ggcaggaagt
    55321 ggcgctggcg cgggaagatg cttccccctg tttacgtttg ggcgcctttg aaatccgccg
    55381 ctatcagtcg caactgtggt ggattaaatc cgtcaccggg caaagcgaaa ccattgtgct
    55441 gtggcagacg tggctacaac cgctggaatt accggcgggg ctgggaaccg tacagcttac
    55501 tgcgggaggc gatattcgcc ctccgcgtgc agacgaagcg gtcagcgtgc gtttcaaagc
    55561 gccaggattg ctgcatattg tcgggcgcaa cggcggacgt aagctgaaga aaatctggca
    55621 agagttaggc gtgccgccgt ggctacgtga caccacgcca ctgctgtttt atggagaaac
    55681 gctgattgcg gcggcagggg tatttgtgac gcaagaaggt gtggctgaag gtgagaatgg
    55741 cgtaagtttt gtctggcaga aaacgcttag ttaagtgaaa gccggataag acgcatcaaa
    55801 cgtcgcatcc ggcgaaagtc aatcaggatt cgcttaccac caccgtaccg atttcagggt
    55861 ggctaaagct ggtaatttta tccagacgca gctcgcgggt ttcgccagcg gcctcgacga
    55921 ccagatactc cacatttttg cgggagacta aatcactggc ttttgcctgc aatttttcgc
    55981 catctttcag ctcaagtgtc agcattaaat gatgctggca ggcgagctca agattatcgt
    56041 aatcatcaca attaattggt tgatacgtat cattcattga cataatcgct caccagtaag
    56101 tttgccgcag cgtatgctgc tttttccctg acagcctcag aaagggcgtc gtcggcagcc
    56161 atttcattca gcactttcaa aacgcagccc agcgcgtccg gaacgtatcc taagtctccg
    56221 ctggcgattt ccgcgtaccg cttgcgtatt aactcacaat atttttccac atgccctcct
    56281 gtcagcactc tgacttaacc gtggatgcaa gtctaagcct acgaagataa actctgtttc
    56341 gcaaggtgac tataccacac tcatttctgc aatatcagcg ccgcaactgc acgtattccg
    56401 ttacaatggc ctcctgattc gaaaggagtt ttcttatggc gcttaaagcg acaatttata
    56461 aagcgacggt taatgtggcc gatctcgacc gcaaccagtt tctcgatgcc tctctgacgc
    56521 tggcgcgcca cccttcagaa acccaggagc gtatgatgct gcgcttgctg gcgtggctga
    56581 aatatgctga tgaacgtctg caatttaccc gtggtttgtg tgccgatgat gagccggaag
    56641 cgtggctgcg taacgatcac ctgggcattg atttgtggat tgagctgggg ctgccggatg
    56701 agcgacgaat taagaaagcc tgcacccagg ccgcagaagt ggcgctgttt acctataata
    56761 gtcgggcggc gcaaatctgg tggcagcaaa atcagagcaa atgtgtgcag tttgccaatc
    56821 tttccgtctg gtatctggac gatgaacaac tggcgaaagt aagcgccttt gccgatcgta
    56881 ccatgacgct gcaggcaacg attcaggatg gtgttatctg gttatcggat gataagaata
    56941 atctggaagt taacttaacc gcctggcaac aaccttcatg attgtgattt cccgacatgt
    57001 tgctattccc gatggtgagc ttgagatcac cgccattcgt gcgcagggcg ctggcgggca
    57061 gcatgttaat aagacctcaa cggctattca tctgcgtttt gacattcggg cgtccagcct
    57121 gccagagtat tacaaagagc gtctgctcgc cgccagccat catttgatca gcagtgatgg
    57181 cgtgattgtc attaaggcac aggaataccg cagtcaggaa ctgaaccgcg aagccgctct
    57241 ggcccggctg gtggcagtga ttaaagattt aacaaaagaa caaaaagccc gacgacccac
    57301 gcggcccacc cgtgcatcga aagagcgcag gctggcatcg aaagcacaaa aatcaagcgt
    57361 gaaggcgatg cgcggcaaag tacgcagcgg tcgggaataa aaagaaggaa tggatggtga
    57421 aaaaagcgat agtgacagcg atggctgtaa tcagcctctt tactctgatg ggatgtaata
    57481 atcgggccga agtcgatacg ctttctccgg cgcaggctgc cgaactgaaa ccgatgccgc
    57541 aaagttggcg cggcgtgcta ccgtgtgccg attgcgaagg aatcgaaatc tctctgttcc
    57601 tcgaaaaaga cggaacatgg gtgatgaatg agcgttatct cggtgctcgt gaagaacctt
    57661 cctccttcgc ttcctacggt acatgggcgc gaaccgctga caagctggta ttaaccgaca
    57721 gcaaaggtga aaagtcatat tatcgggcga aaggcgatgc gctggagatg ctcgatcgtg
    57781 aaggcaatcc aattgaatcg cagttcaact atacgctgga accggcacaa tccagtttac
    57841 ctatgacgcc gatgaccctg cgaggcatgt atttttatat ggctgatgcg gcgaccttca
    57901 ctgattgcgc gaccggaaaa cgtttcatgg tagcgaataa cgcagagctg gagcgtagct
    57961 acctggctgc gcgcggtcac agtgaaaaac cggtgttact gtcagtagaa ggtcacttta
    58021 cgcttgaggc taatccggat actggtgcgc caaccaaagt attagcgccc gatacggcgg
    58081 gtaaatttta cccgaataag gattgcagta gtttggggca gtaaacctgc ttaaggcaga
    58141 aacaaacgca aaattgcctg atgcgctgcg cttatcaggc ctacagcata taatgcaaca
    58201 ttttgaatta gcacgatttt gtaggccgga taaggcgttt acgccgcatc cggcattttg
    58261 tgcacgaggc tggctttatc actgagtcag cccaacaaag cgacccgcct tgatataaat
    58321 cccaggcttc aggtgcccaa catactgtaa ctgcgtttct ggcttaaacg cagagacatc
    58381 accaatacgc atatgcatca gatcggcagg gctaatccag cccgattgcg ccagtgtaag
    58441 tggatgcccg gatttggcaa atgcatcagt gacaaattcc gaacaaaacc acgacttttt
    58501 gtctccttcg cacacactgc ttaactgcgc tttcgccagg tcgctgacgc actgttggcg
    58561 aaaatcctcg gagaacggat tcagtgagca catctggcga gtcaccataa agggaataaa
    58621 ttcgacaatg ccgcgatagt tataaccgct atctttgatt ttattggcaa acgcggtgat
    58681 atctgtggct tgttgcgggg taagatccgg gactcgtaag acgaaaagct tatcactatg
    58741 cttcatcgct tttttaaggg aaacaatctg gacgccagcg cctgtcgctt ctgcaacgtt
    58801 attatcaccc agaaagattg caacgtgact cacagaggaa gtgctgaaga cgcggattcc
    58861 aaatgaggtt accccaaggc ttgaggagaa cagcaaatcg ccgggtttga gatcaggtgc
    58921 cgttatttct tttattgatt gttcggtgaa agagctttga tgctggaatt tgacagccca
    58981 tgtttttgcc tcggcatcta ctgctgtggc tgatgggtct ggctggctga tatcaacggt
    59041 acaagccgaa agtaaaagaa agctggggag aagcaggcgg cagtacgcct ttggtttatc
    59101 cattttatac aatccatgta aaaaagggcc ctgaaattca ggaccctttc tggcatcagc
    59161 ctttaatctg tttcaccaga tattcgacga tgtcaccagt cttaattaac tgtttctcgc
    59221 cgttgcgacg atatttatat tcgatatcgt cgttgtcgag gttacggtcg cccagcacaa
    59281 tagtgtgcgg aataccgatc agttccatat cagcaaacat cacgcccgga cgctctttgc
    59341 ggtcatccag cagcacttcg ataccttgtg cacgcagttc gctgtacagt ttctcagcaa
    59401 gctcttgtac gcggaaggat ttgtgcatgt tcatcggcag aatcgccacc tggaacggcg
    59461 cgatagcgtc aggccatacg atgccgcgtt cgtcgtagtt ctgctcaatc gccgcagcta
    59521 ccacacgcgt taccccgata ccgtagcaac ccatcgtcag gatttggtta cggccatctt
    59581 caccctgtac ggaggctttc agtgcttcgg agtacttggt acccagctgg aagatgtgac
    59641 caacttcgat accacgtttg atcagcagcg taccctggcc atccgggctt ggatcgccag
    59701 ccaccacgtt acggatatct gcaacttccg gggtagcgac atcgcgatcc cagttgatac
    59761 cgaagtagtg tttaccatcg atgttagcac cagcagcgaa atcactcatc gccgcaacgg
    59821 tacggtcaat caccaccgga atcggcatgt ttaccggacc cagtgaaccc ggaccggctt
    59881 taaccacagc acgaatttct tcttcggtcg cgaaagtcag cgggcttgca acctgcggca
    59941 gtttttctgc tttaacttcg ttcagctcgt gatcaccgcg caccagcagc gcaaccagcg
    60001 ggaagctact gccttcaacc gctttaacca gcagagtctt aaccgttttc tcaatcggca
    60061 gattgaactg ttcaaccagt tccgcgatgg ttttcgcgtt cggcgtatca accagcgtca
    60121 tttcctgggt agcagcagcg cgcggttctt tcggcgcgat agcttctgcc agttcaatgt
    60181 tcgctgcata gtcagaggtg tcggagaaga ccacatcgtc ttcaccgctc tgcgccagca
    60241 cctggaattc gtgagaggcg ctgccgccga tagaaccggt gtcggcctgt acggcgcgga
    60301 aatccagccc catgcggctg aagattttgc tgtaggccgc atacattgca tcgtaggttt
    60361 cctgcaggga ttcctgagaa gtatggaaag agtaagcatc tttcatcagg aattcgcggg
    60421 aacgcatgac gccgaaacgc ggacgcactt cgtcgcggaa cttggtctgg atctgataga
    60481 agttcagcgg cagctgtttg taagagctaa gctcgttacg aatcaggtca gtgataactt
    60541 cttcatgagt tgggccgagt acgaacggac gctcgccacg gtcaacaaaa cgcagcagtt
    60601 ccggaccgta ctgttcccaa cgaccactct cttgccacaa atcggctggc tgaaccaccg
    60661 gcatcgacac ctcgatcgca ccggcgttgt tcatctcttc acgcacgatg ttttcgactt
    60721 ttttcagaac gcgcacgccg gtcggcagcc aggtatataa cccggaggcc agcttgcgga
    60781 tcatcccggc gcgcagcatc agctgatggc tgatcacctc ggcgtcggca ggtgtctcct
    60841 tgagagtgga gagcaggtat tggctagtac gcatgttgtt acggttccag ttggaaggta
    60901 gaacaggctc aaggcgagcc tgggacaaaa aaagtgattt agtttaccag tgcaaaagaa
    60961 atgtcaaaag agaagggcgt gaatttaacg cggttccagc gcaaagactt caaaacctgc
    61021 gtcggtgacg cgccagcgaa cgttaaaatc atgtagccag acggcatagg ttttgcccgt
    61081 ttcctcacct ttacgatagg ccgggcgcgg gtcctgcgcc agtacttcgc ggataaacag
    61141 cgttaactgc ggataacgct tctccagcgt caaaagctgc ttttcgacct ctgcggtaaa
    61201 actcaccgcc atctctgcag ctggcgcact ttgcgcatag ctggcactgg catcgggaag
    61261 cgattcggca aaggggagat acggtttgat atccactacc ggcgtaccat cgaccagatc
    61321 cagactgccg agcttcagaa tcacgctgcc tttatggcaa acaacctctt tcagctctac
    61381 cagcgacatg ccaattgggt tagggcggaa agtagagcgt gttgcgaaaa cccccattct
    61441 ggcgttaccg ccgaggcgcg gtggacgcac agtcggacgc cagccgcctt ccatcgtttg
    61501 atgaaagacg aaaaggatcc ataaatggct gaatgcttcc aggccgcgaa cggcgtcggc
    61561 ctggttgtag gaagcaatga gatgcagttc gccgttggcg ctttttacca gacctggctg
    61621 gcgcggaacg gcgaattttt ctttataggg cgagcgaata acgcctattt gctcgaactg
    61681 gaaactgctc atttcgccgt aatgttaagc gcagagccga tacatacagc ctgacgatag
    61741 cagcccggcg taccgctggt gacttcgcag ctatgcagta atacagcatt ggctttcatt
    61801 ttagaggcat tgatttgcat ctgcttacgt gcggttggaa tgctcggcgg agagtcctga
    61861 ttagaggcct ggcaagagtc gccactgact tcaccgagat cgcggaacgg tttgccgact
    61921 aattcttctg cattggtata aattcggacc ggcgtggcgc gcggcgcttt cggttttgca
    61981 ggctccgctt tcggctgggg tgcagtgctt tgaacgggtt cgacagggga tctgcttaac
    62041 atggaacagc cgcttagcat gagtgctact aaacagatcg gtaaagcacg catagtattt
    62101 cctcaatgta tgatcaaaac gtcaatattg aatcaggagc ttgtaaaaat gacaagacgg
    62161 gcaagcgccc gtcctgaatg atattacaaa ttgtggaaac agcctaaaaa ttaccagcct
    62221 ttaacagcgc cgccgttaaa cactttgttt gctgcttcgt aaacttcatc agactgataa
    62281 gcctggacga atttcttcac gttctcggcg tctttgttat cttcacgcgt tacgatcagg
    62341 tttacgtatg gggagtcttt atcttcgaca aagataccgt ctttcgccgg agtcaggcca
    62401 atctggctgg cataggtggt attgataact gccagagcga tttgcgcgtc gtccagagaa
    62461 cgcggcagtt gcggtgcttc cagttcaaca attttcagat tttttgggtt ctcaacaaca
    62521 tcaagaacgg tcggcagcag gccaacgcca tctttcagtt tgatcaagcc cactttttgc
    62581 agcagcagca gtgaacgacc aagattagtt ggatcgtttg gcacggcaac ctgcgaacca
    62641 tcctgcagtt catccagtga tttgattttc ttggagtaac cagcaatcgg ataaacaaaa
    62701 gtgttgccta ctgcgaccag tttgtagcca cgatctttca gttgctgatc aaggtacggt
    62761 ttatgctgga aggcgttggc gtcgatatcg cctttgctca atgcttcgtt tggcagaaca
    62821 tagtcgttga aggttaccag ctcaacgtcc aggccatatt tgtctttcgc aactttctgc
    62881 gcgacttctg ctacctgctg ttcggcacca acaatcacgc cgactttaat gtggtttgga
    62941 tctttttcat cctgaccgca gcctaccagt gccagtgatc caatcagggc tcccactgcc
    63001 gcaaaggttt tgaatttgaa cgccatacct tattccttct tcaattattt atgttgtgtt
    63061 gaacgttact tgcgagtgac agcccggacg atgcggtcgc ctgcgaactg aattaaataa
    63121 accagaatga ccagcaatac cagtaccgta ttcatcaccg tcgcgttgta gccgatgtag
    63181 ccatactgat agccaatctg acctaaacca ccggcaccga ctgcaccacc catcgcggaa
    63241 taaccgacca gggtaatcag ggtgatagtt gccgcattca ccagacccgg caacgcttcc
    63301 ggtaacagca ctttacagac gatctgcatt ggcgtggcac ccattgcgcg ggaagcttca
    63361 attaacccgg ttgggatctc cagcagagcg ttctcgacca tacgggcaat aaacggtgct
    63421 gcaccaacgg ttaacggaac aattgctgcc tgcaaaccaa tcgatgtacc gacaataacg
    63481 cgggtaaacg gaatcatcca taccagcaag ataatgaacg ggatggaacg gaaaatgttc
    63541 acaatcgcag aaacggtacg atacagcttc gcgttagcaa taatttgccc cggacgcgtg
    63601 acataaagca gaacgccaac cggcagacca atcacaaacc caaaaaatcc ggatacgaag
    63661 gtcattgcca gcgtttccca tacgccacga accagcagcc acatcatcgg ctcagacata
    63721 acccagtacc tctactttta catggtgttc ctgcagccag gcaatggcgg cttgcgtatc
    63781 ttgttgtgtg ccgtgcattt cagtcagcat gatgccgaat ttaacgccac cggcgtagtc
    63841 catctgcgcg ctaataatgt tgttgttgac gttgaaacga cgcgcggttt cagaaagcag
    63901 tggggcatcg accgattgac cggtaaactc cagacgcagc atcggcacgc agtcagtaaa
    63961 tggctccgtt tgcagacgtt cctggtaatc ttccgggata tccagatgca gggtcgactg
    64021 aataaacttc tgcgccagcg gcgttttcgg atgcgagaac acttcactta ccgtgtcctg
    64081 ctcgatcagt tcgccattgc tgatgaccgc cacgcaatca caaatacgct tcacaacgtc
    64141 catttcgtgg gtgatcaaca gaatcgtcaa acccagacgg cggttaatgt ctttcagcag
    64201 ttcgagaata gaacgtgtcg ttgccgggtc cagcgcgctg gtggcttcat cacacagcaa
    64261 tactttggga ttgctggcta atgcacgggc aatcgccaca cgttgtttct gcccaccgga
    64321 aagattcgac gggtagctat catgcttatc gccaagacca accaatgaca gcaattccgt
    64381 cacgcgacgt ttgatctcgt ctttcggtgt gttgtccagc tccagcggca gagccacgtt
    64441 gccaaaaaca gtacgcgaag agagcaggtt aaaatgctgg aaaatcatac caatctggcg
    64501 gcgagctttg gtcaactcgg attctgacag cgtggtcaat tcctggccat cgaccagcac
    64561 gctaccctcg gttgggcgct ccagcaggtt tacacaacgt ataagcgtac tcttacccgc
    64621 gcctgaggca ccgataacgc cataaatttg tccagctggc acatgcaggc tgacgttgtt
    64681 caacgcctgg atggtgcggg tgccctggtg gaacactttg gtgatattcg aaagttttat
    64741 cattgattat ttattatcgt cattaagtta gtcgtagcat ctcgaatgcc tgaaacgggc
    64801 aacgccgtca atgaaatgga tgttaaggca tccagacgtc taaatcaatc aggtttatgc
    64861 gaagagcact ttcttgcagg tcgaaacatg cgatactagc gtcccatgcc ttattaagga
    64921 gctataaaag gtggcgaaga gcgtacccgc aatttttctt gaccgtgatg gcaccattaa
    64981 tgtcgatcac ggctatgtcc atgagatcga caactttgaa tttatcgacg gtgttattga
    65041 cgccatgcgc gagctaaaaa aaatgggctt tgcgctggtg gtagtaacca accagtctgg
    65101 cattgctcgc ggtaaattta ccgaagcaca gtttgaaacg ctgaccgagt ggatggactg
    65161 gtcgctggcg gaccgagatg tcgatctgga tggtatctat tattgcccgc atcatccgca
    65221 ggggagtgtt gaagagtttc gccaggtctg cgattgccgc aaaccacatc cggggatgct
    65281 tttgtcagca cgcgattatt tgcatattga tatggccgct tcttatatgg tgggcgataa
    65341 attagaagat atgcaggcag cggttgcggc gaacgtggga acaaaagtgc tggtgcgtac
    65401 gggtaaacct attacacctg aagcagaaaa cgcggcagat tgggtgttaa atagcctggc
    65461 agacctgccg caagcgataa aaaagcagca aaaaccggcg caatgattaa aagatgaacg
    65521 gttgaaataa aaatgcattt ttccgcttgt cttcctgagc cgactcccta taatgcgcct
    65581 ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt gaagagaaaa
    65641 aatcctgaaa ttcagggttg actctgaaag aggaaagtgt gatatacgcc acctcgcgac
    65701 agtgagctga aagctgcgtc gcaactgctc tttaacaatt tatcagacaa tctgtgtggg
    65761 cactcgaaga tacggattct taacgtcgca agacgataaa tgaataccaa gtctcaagag
    65821 tgaacacgta attcattacg aagtttaatt ctttgagcat caaactttta aattgaagag
    65881 tttgatcatg gctcagattg aacgctggcg gcaggcctaa cacatgcaag tcgaacggta
    65941 acaggaagcg cttgctgctt cgctgacgag tggcggacgg gtgagtaatg tctgggaaac
    66001 tgcctgatgg agggggataa ctactggaaa cggtagctaa taccgcataa cgtcgcaaga
    66061 ccaaagaggg ggaccttcgg gcctcttgcc atcggatgtg cccagatggg attagcttgt
    66121 tggtggggta acggctcacc
//
LOCUS       DAEEGA010000010        65614 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEEGA010000010 DAEEGA010000000
VERSION     DAEEGA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65614
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.010"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="KLH46_000656"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="KLH46_000656"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBE0377467.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="KLH46_000657"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="KLH46_000657"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBE0377468.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="KLH46_000658"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="KLH46_000658"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBE0377469.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="KLH46_000659"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="KLH46_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBE0377470.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="KLH46_000660"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="KLH46_000660"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBE0377471.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="KLH46_000661"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="KLH46_000661"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBE0377472.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="KLH46_000662"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="KLH46_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBE0377473.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="KLH46_000663"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="KLH46_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBE0377474.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="KLH46_000664"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="KLH46_000664"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBE0377475.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="KLH46_000665"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="KLH46_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBE0377476.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="KLH46_000666"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="KLH46_000666"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBE0377477.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="KLH46_000667"
     CDS             complement(15665..16702)
                     /locus_tag="KLH46_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBE0377478.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="KLH46_000668"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="KLH46_000668"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE0377479.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="KLH46_000669"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="KLH46_000669"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBE0377480.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="KLH46_000670"
     CDS             19444..19884
                     /locus_tag="KLH46_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBE0377481.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="KLH46_000671"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="KLH46_000671"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBE0377482.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="KLH46_000672"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="KLH46_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="KLH46_000673"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="KLH46_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBE0377483.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="KLH46_000674"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="KLH46_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBE0377484.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVMLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="KLH46_000675"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="KLH46_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HBE0377485.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="KLH46_000676"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="KLH46_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBE0377486.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="KLH46_000677"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="KLH46_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBE0377487.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="KLH46_000678"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="KLH46_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBE0377488.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="KLH46_000679"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="KLH46_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBE0377489.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="KLH46_000680"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="KLH46_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBE0377490.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="KLH46_000681"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="KLH46_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBE0377491.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="KLH46_000682"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="KLH46_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBE0377492.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEELLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="KLH46_000683"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="KLH46_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBE0377493.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="KLH46_000684"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="KLH46_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBE0377494.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="KLH46_000685"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="KLH46_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBE0377495.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="KLH46_000686"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="KLH46_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBE0377496.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="KLH46_000687"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="KLH46_000687"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBE0377497.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="KLH46_000688"
     CDS             37412..37681
                     /locus_tag="KLH46_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBE0377498.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="KLH46_000689"
     CDS             complement(37684..38043)
                     /locus_tag="KLH46_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBE0377499.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="KLH46_000690"
     CDS             38184..38810
                     /locus_tag="KLH46_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBE0377500.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="KLH46_000691"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="KLH46_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBE0377501.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="KLH46_000692"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="KLH46_000692"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBE0377502.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="KLH46_000693"
     CDS             41650..42315
                     /locus_tag="KLH46_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBE0377503.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="KLH46_000694"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="KLH46_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBE0377504.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="KLH46_000695"
     CDS             complement(42949..44166)
                     /locus_tag="KLH46_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0377505.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="KLH46_000696"
     CDS             44300..45349
                     /locus_tag="KLH46_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBE0377506.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="KLH46_000697"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="KLH46_000697"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBE0377507.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="KLH46_000698"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="KLH46_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBE0377508.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="KLH46_000699"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="KLH46_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBE0377509.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="KLH46_000700"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="KLH46_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBE0377510.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="KLH46_000701"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="KLH46_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBE0377511.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="KLH46_000702"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="KLH46_000702"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBE0377512.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="KLH46_000703"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="KLH46_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBE0377513.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="KLH46_000704"
     CDS             complement(51544..51903)
                     /locus_tag="KLH46_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBE0377514.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="KLH46_000705"
     CDS             complement(51900..52175)
                     /locus_tag="KLH46_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBE0377515.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="KLH46_000706"
     CDS             complement(52234..53358)
                     /locus_tag="KLH46_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0377516.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="KLH46_000707"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="KLH46_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBE0377517.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGLGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="KLH46_000708"
     CDS             complement(56090..57157)
                     /locus_tag="KLH46_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE0377518.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57523..59145)
                     /locus_tag="KLH46_000709"
     CDS             complement(57523..59145)
                     /locus_tag="KLH46_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBE0377519.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKDYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTKPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKYAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59407..60996)
                     /locus_tag="KLH46_000710"
     CDS             complement(59407..60996)
                     /locus_tag="KLH46_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBE0377520.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61381..62412
                     /locus_tag="KLH46_000711"
                     /pseudo
     CDS             61381..62412
                     /locus_tag="KLH46_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62499..62975)
                     /locus_tag="KLH46_000712"
     CDS             complement(62499..62975)
                     /locus_tag="KLH46_000712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377521.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62968..63732)
                     /locus_tag="KLH46_000713"
     CDS             complement(62968..63732)
                     /locus_tag="KLH46_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBE0377522.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEIFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63978..64739
                     /locus_tag="KLH46_000714"
     CDS             63978..64739
                     /locus_tag="KLH46_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE0377523.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDSFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64846..65346)
                     /locus_tag="KLH46_000715"
     CDS             complement(64846..65346)
                     /locus_tag="KLH46_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377524.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
BASE COUNT        15939 a        17620 c        16160 g        15895 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgcag aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgacacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcaacacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc atgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg gagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggctagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaagatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcgag aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag ctcttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat caggttaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aatagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctggcgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatagcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtgca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgcc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctgaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc gcgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatcg cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttt cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caagcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcactt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggccggaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tatttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcttggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatc
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaagatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca
    59581 tttgaagata tcttcaggtt ttggtctgaa agcagtagtg ttattaaaat aattttcata
    59641 tacaggtgtg ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg
    59701 ttgtttatgt tttttattac atatatttgc taattcctca ggatcaattg ggtctttcat
    59761 ctgattaaat ggaacctgca aagcaccgag acaataacat tgattttctt catttcttat
    59821 tattccgtga tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt
    59881 tttaattaag ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata
    59941 gttaccatta aaattgattt catgatttcc agccagaacg atgacatttt tattaatacg
    60001 gctatcttta ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc
    60061 accaaaaata gtactaaatc tatcgcctgt atgatcccca aggaagatac aaggggttat
    60121 cgcatcgtcc tttaaaaaaa catgttgatg caactcattc aatatcatgt ctctcgtatt
    60181 taataaattg tagagcaatt tattattttt atgtatgtat gcagatgaag ccatgtcttc
    60241 gtgttttaat aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga
    60301 aacgagcaat gaataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt
    60361 gttggcattt tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac
    60421 agatttaaca tagctatcaa acttatcctt aggaaacgtc tctttagaga catcccaacg
    60481 ccagtcagct ttaaaattac cgctaagaag agtaaagaaa ttttcaggta tgatagaaag
    60541 cccattaact ttataaatta tgtctgattt atctccggag aaattaataa aataataacc
    60601 atccttacac tcaatcttaa ctgtaatggg agacgataat tttatcacat ggttattgtc
    60661 tggtataatt gtaaagataa acgtgttagt agtattatcg gcaaaactat tatattcttg
    60721 aatatctata gatggctcac tattgtaaaa ccatattttc tccatctgaa gactatgttc
    60781 atttaccctc cagtgaccat gttgcaggaa atagatgata tgcatgccaa aatcgttatc
    60841 tatttgcgaa tcattatcca gcttatcaat ttcaactatc tttgaacgat gtgttttatc
    60901 aaccttgcta tggtgttgat gattattaac tttatctgaa tgcttatctt tatggtttga
    60961 agggatgtac ttaaatatat tgtcaattgc tttcataaat aacccgcgat atttttgacc
    61021 tgttagtaaa aaataaatga tacatgtctg aaagacatat ttacgacgat ataaaacaat
    61081 aatacttata tgttcgctga ttttaagcac cgagactatc cggtctttta ctaaataatg
    61141 gaaaacaatc catccctgga agacaattgt aaaaaaaaac aacagaataa gaattaatct
    61201 taggataaat atttatttat catggccttt tgaccgctaa aaaatggcac tcggcacagt
    61261 aaaccccgct atagttatgc tatgcatttg tctttaatga tatctcgtcg ttatattaac
    61321 aggctgaaat tatcattaat gcacatttca atattagcag ggatacctga ggagtttaaa
    61381 gtgaacatat atatcgggta gcttttcaaa ttaatccctt tgattatagg cttaatttgc
    61441 atcgcgttag gtggctttgt gctggaaagt tcagggcaac gtcacgggtc atgtgctgat
    61501 ttctctggcg gccatatgcc tggcattatt cactaccgca tttattatca tttcgcaact
    61561 cacgcgcggc gttaatacgt tttacaatac attgttcccc attattggct atgcggggtc
    61621 aattatcacc atgatatggg gttgggcact gttagcaggt aatgatgtga tggcagacga
    61681 gtttgttgcc ggccatgtta tttttggcgt tggtatgatt gccgcatgtg tatcgacggt
    61741 ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat gcagcgggga gcaagagcga
    61801 cggaacaccg gtacaggctt attcttcatt aatcggtaat tgcctcattg ccgttcccgt
    61861 tttactcacc ctgctcggtt tcatttggtc tattacgctg ttacatagtg ccgacataac
    61921 tccgcattat gtcgccttgg gttaaccgca atctgtgcct gtctaattgg cctagtggcc
    61981 acaatcgtcc atcaaacacg taatacgttc tcatccaaag agcactggct gtggtgttat
    62041 tgggttattt ttctcggctc agtcacggtg ctgcagggga tatacgtctt agtcagttcc
    62101 gatgcaagcg cccgactggc tcccggcatt attctcattt gcctcggaat gatctgttac
    62161 agcatattct caaaagtctg gctactggca ctggtctgga gacgtacctg ttcgttagcc
    62221 aacagaatac cgatgatacc ggtatttacc tgtctgtttt gcttgttttt gtcatcattc
    62281 ctcgcggaaa tcgcacaaac tgagctggac tactttattc catctcgggt tctggttgga
    62341 ttaggcgcag tatgttttac attgttctct attgtttcaa tattagaggc cggttctgcg
    62401 aaaaaaaatt aaaatttaca taatcccagc gttattcata tcttaacgct ggttttttac
    62461 gaacttagtg aaataatgta gaatataaaa ttataatttt atattggagc acatgtgtta
    62521 atgatcaacc ccgggatact aaaaatatag acattattaa acttgcttag cctcgcattg
    62581 cctttacctg ttaaagatgc atcgagaaaa ttgagtctga agttctcatc ctttatctga
    62641 ttttccagtc ccgaaacttt ggccttagca ttacctgaga tccgtccgtt actatctatt
    62701 ttgtaggtaa aaaaatacga atgggaaaat ttcctcggtg tatcttcatc agttaagcca
    62761 acaatcgtaa aactaccgct accatctttt tttaaaatga ctgaaaggtt agcagagtag
    62821 tataaagaat gatctgtttt agcatttctt gttaacgtcg acgtacattc aagagacgtt
    62881 gtgctaccat cacgagcctt ccagagagca aaaaaacaca cctgtagcaa tgtgaaaaca
    62941 agtattaata taaaatacat tttcttatta gtcatataat agcacctctg aacaaattgc
    63001 ataagtttcg tcattagagg attttataca tcttgataaa taccgctgcc cttccccaag
    63061 caaataatgc atactattta catcatatat aaaatttgca cctggcacac agggtaaatg
    63121 ctttttcgaa atttcagcgg cctctatttg agatttagcg aggccaatct cgttaagatt
    63181 ctctgaaaga tataaatcac aggtgtcaat cttacctgat ttaaccaatg gataatccgt
    63241 tatactgtag gtatctttaa aagccacggc aaatatttca ataataacaa ttactaccac
    63301 aagcgaaatc aaaatgcgaa aaagaggaat actccttttg cgctctactt tttcaacaac
    63361 atcttcttgt aaatgagaat catcataaga ttgtttactt attgttatgt tttcactgat
    63421 gtaaaagcct actttgggta tcgtaagaat cacattttct tcaacaccaa tatttttaaa
    63481 acttttgcgt aacagggata tatattggtt caatgtgttg tttgacagat caaatgaaaa
    63541 tctctccaaa acgctatcaa tgattgagtt cctgtcaacc gctccaccat tactttccag
    63601 taacactgcc aaaatacacc ccgccgtagc tggcaaattt atctcatcct gggtatttac
    63661 attcgagagc gagcatttgt tcggatcaaa aataatcgcg tcattgataa tgaaaaaaca
    63721 tctccgctcc atgcaggcaa caacacaaaa taagattttg tcattttagc ctattcctgg
    63781 tggaactgac acaagaaact aaaatacaaa tataaccctg aaaaagtcat ataaaactta
    63841 tatccacaga ataaaaaatc aaacatgtca gttttatcaa tataaagtat cgcaaggcga
    63901 aaagtgatgc aagaatactc ctaagtaaat gaaagcgcac cacatgaaat tatcaacaca
    63961 tgtaatttca tcattaggtg ctatcgaaaa taactcagga gctacttaag atgaataatg
    64021 tcttactcat gagacccgat gactcagaat ttatgatacc tgatgactgg tcagggctaa
    64081 acgcaaaagg cattacatac gaacaagata atagagtgac agagtctttt ctgcctttta
    64141 aatcttttta ttgttcaccg gtgtgcacga cagttgaact catctcacaa aataattgtt
    64201 ttgctatggg tatccgctac cttctggaaa agaaaagaga cttaaggcta aatcacttta
    64261 aagccttctc tcataaatgc gagacaaaac ctgacgtgtt aattatgacc gggtgcaccg
    64321 tcgctgatac ttatagtata ttctcacgca ttgttgaaga aaatataatg aaaacgatga
    64381 cacctatcgt catcattact gggcatcact cagaagatct ttgttctctg gcatcgaaac
    64441 tgggaggagt taaaataata tcaccgacag aaaagattgc aaaaatagaa aacgctatca
    64501 atgattcttt taacagcaag cctaaaaaaa taagattttt aacagatata ctctctcctc
    64561 aacaaattga gaccatgaaa atggttgcgc agggtagatc cgttaatgag atttcagccg
    64621 caatgaatat tagcatcaag accgtgtatg cacataaagt tcgtgctatc aataagctgg
    64681 gaattaagaa aaaagtttat gaagcgcttt tttataaagc gctaattcag ttcaactgat
    64741 gtaaaaatca ccctcgctta ataaataata tattctatgt ctttttttat aagttaaaaa
    64801 aggcgggata tcaaaccccg ccatgaaatt aactaatcat ctgcattatt ctgcgtagat
    64861 ataagcctgg gtacggatgg tgtaagagtc tggctcaaca acctgttctt tcatatggga
    64921 aacgacgcga taggcaccac ctgttccagt atttttcagc atcatataac gcttaccgcc
    64981 aatctcttcg gaggcagctg aaggaggatc ctgctcaagc gttgtcaggg aaactttcag
    65041 tttatggtcc gggttattgt caccagaaat tactgcatcc gctgaagtac tgctctccgc
    65101 ataaatatca tccgggaaag ttacagcata gtcgtggctc tgcccatcag atgcgccaac
    65161 aatacctgtt gccaaaggcg tccatgcagt taacgtaccg ctatataccc ctttcactgg
    65221 ttgaatggta ttattaacaa cgataggctt ggtcaaagta agattagctg acgcttcagt
    65281 accctggctt actaaagttc ccgcctgaga agcaaaagct gtccccgcca gcatgattgc
    65341 taacaatgtt ttcttcatta tattttctcc atgattttca tttacactgc aaaatgcagt
    65401 ttctttcttt gcatttacgt gctatcgcga aaatgcttcc accaaataat ggaaacatct
    65461 tcacaaaaat aaattatgag tttcttaatt tagaaactat aaacatgtcg ccgctatctg
    65521 gtataaccct gatgcacttt tcttcgcagg aagttggtat tttgcgatac aggattgatc
    65581 tttagaataa ccccatttca cattaaactc gcct
//
LOCUS       DAEEGA010000011        65445 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEEGA010000011 DAEEGA010000000
VERSION     DAEEGA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65445
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.011"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            131..574
                     /locus_tag="KLH46_000716"
     CDS             131..574
                     /locus_tag="KLH46_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0377525.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /gene="ybhA"
                     /locus_tag="KLH46_000717"
     CDS             810..1628
                     /gene="ybhA"
                     /locus_tag="KLH46_000717"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HBE0377526.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="KLH46_000718"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="KLH46_000718"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBE0377527.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="KLH46_000719"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="KLH46_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBE0377528.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="KLH46_000720"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="KLH46_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0377529.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="KLH46_000721"
     CDS             complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="KLH46_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBE0377530.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="KLH46_000722"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="KLH46_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBE0377531.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="KLH46_000723"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="KLH46_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBE0377532.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCHALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="KLH46_000724"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="KLH46_000724"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBE0377533.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="KLH46_000725"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="KLH46_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBE0377534.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="KLH46_000726"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="KLH46_000726"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBE0377535.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="KLH46_000727"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="KLH46_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBE0377536.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="KLH46_000728"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="KLH46_000728"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBE0377537.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="KLH46_000729"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="KLH46_000729"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBE0377538.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="KLH46_000730"
     CDS             13934..14314
                     /locus_tag="KLH46_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBE0377539.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="KLH46_000731"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="KLH46_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBE0377540.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="KLH46_000732"
     CDS             complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="KLH46_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBE0377541.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="KLH46_000733"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="KLH46_000733"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBE0377542.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17415..17490)
                     /locus_tag="KLH46_000734"
     tRNA            complement(17415..17490)
                     /locus_tag="KLH46_000734"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Lys,seq:ttt)
     gene            complement(17494..17569)
                     /locus_tag="KLH46_000735"
     tRNA            complement(17494..17569)
                     /locus_tag="KLH46_000735"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17534..17536),aa:Val,seq:tac)
     gene            complement(17621..17696)
                     /locus_tag="KLH46_000736"
     tRNA            complement(17621..17696)
                     /locus_tag="KLH46_000736"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17661..17663),aa:Lys,seq:ttt)
     gene            complement(17699..17774)
                     /locus_tag="KLH46_000737"
     tRNA            complement(17699..17774)
                     /locus_tag="KLH46_000737"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17739..17741),aa:Val,seq:tac)
     gene            complement(17911..17986)
                     /locus_tag="KLH46_000738"
     tRNA            complement(17911..17986)
                     /locus_tag="KLH46_000738"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17951..17953),aa:Lys,seq:ttt)
     gene            complement(18151..18942)
                     /gene="cpoB"
                     /locus_tag="KLH46_000739"
     CDS             complement(18151..18942)
                     /gene="cpoB"
                     /locus_tag="KLH46_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBE0377543.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18952..19473)
                     /gene="pal"
                     /locus_tag="KLH46_000740"
     CDS             complement(18952..19473)
                     /gene="pal"
                     /locus_tag="KLH46_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBE0377544.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19508..20800)
                     /gene="tolB"
                     /locus_tag="KLH46_000741"
     CDS             complement(19508..20800)
                     /gene="tolB"
                     /locus_tag="KLH46_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBE0377545.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20933..22174)
                     /gene="tolA"
                     /locus_tag="KLH46_000742"
     CDS             complement(20933..22174)
                     /gene="tolA"
                     /locus_tag="KLH46_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBE0377546.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22239..22667)
                     /gene="tolR"
                     /locus_tag="KLH46_000743"
     CDS             complement(22239..22667)
                     /gene="tolR"
                     /locus_tag="KLH46_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBE0377547.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22671..23363)
                     /gene="tolQ"
                     /locus_tag="KLH46_000744"
     CDS             complement(22671..23363)
                     /gene="tolQ"
                     /locus_tag="KLH46_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBE0377548.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23360..23764)
                     /gene="ybgC"
                     /locus_tag="KLH46_000745"
     CDS             complement(23360..23764)
                     /gene="ybgC"
                     /locus_tag="KLH46_000745"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBE0377549.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23914..24207)
                     /gene="ybgE"
                     /locus_tag="KLH46_000746"
     CDS             complement(23914..24207)
                     /gene="ybgE"
                     /locus_tag="KLH46_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBE0377550.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24207..24320)
                     /gene="cydX"
                     /locus_tag="KLH46_000747"
     CDS             complement(24207..24320)
                     /gene="cydX"
                     /locus_tag="KLH46_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBE0377551.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24335..25474)
                     /gene="cydB"
                     /locus_tag="KLH46_000748"
     CDS             complement(24335..25474)
                     /gene="cydB"
                     /locus_tag="KLH46_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBE0377552.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25490..27058)
                     /gene="cydA"
                     /locus_tag="KLH46_000749"
     CDS             complement(25490..27058)
                     /gene="cydA"
                     /locus_tag="KLH46_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBE0377553.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27907..30540)
                     /gene="mngB"
                     /locus_tag="KLH46_000750"
                     /pseudo
     CDS             complement(27907..30540)
                     /gene="mngB"
                     /locus_tag="KLH46_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(30558..32474)
                     /gene="mngA"
                     /locus_tag="KLH46_000751"
     CDS             complement(30558..32474)
                     /gene="mngA"
                     /locus_tag="KLH46_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBE0377554.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32644..33366
                     /gene="mngR"
                     /locus_tag="KLH46_000752"
     CDS             32644..33366
                     /gene="mngR"
                     /locus_tag="KLH46_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBE0377555.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33469..34338)
                     /gene="sucD"
                     /locus_tag="KLH46_000753"
     CDS             complement(33469..34338)
                     /gene="sucD"
                     /locus_tag="KLH46_000753"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBE0377556.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34338..35504)
                     /gene="sucC"
                     /locus_tag="KLH46_000754"
     CDS             complement(34338..35504)
                     /gene="sucC"
                     /locus_tag="KLH46_000754"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBE0377557.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35598..36815)
                     /gene="odhB"
                     /locus_tag="KLH46_000755"
     CDS             complement(35598..36815)
                     /gene="odhB"
                     /locus_tag="KLH46_000755"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBE0377558.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36830..39631)
                     /gene="sucA"
                     /locus_tag="KLH46_000756"
     CDS             complement(36830..39631)
                     /gene="sucA"
                     /locus_tag="KLH46_000756"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBE0377559.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39932..40648)
                     /gene="sdhB"
                     /locus_tag="KLH46_000757"
     CDS             complement(39932..40648)
                     /gene="sdhB"
                     /locus_tag="KLH46_000757"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBE0377560.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40664..42430)
                     /gene="sdhA"
                     /locus_tag="KLH46_000758"
     CDS             complement(40664..42430)
                     /gene="sdhA"
                     /locus_tag="KLH46_000758"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBE0377561.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42430..42777)
                     /gene="sdhD"
                     /locus_tag="KLH46_000759"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42430..42777)
                     /gene="sdhD"
                     /locus_tag="KLH46_000759"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBE0377562.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42771..43175)
                     /gene="sdhC"
                     /locus_tag="KLH46_000760"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42771..43175)
                     /gene="sdhC"
                     /locus_tag="KLH46_000760"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBE0377563.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43556..43663
                     /locus_tag="KLH46_000761"
     CDS             43556..43663
                     /locus_tag="KLH46_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377564.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43869..45152
                     /gene="gltA"
                     /locus_tag="KLH46_000762"
     CDS             43869..45152
                     /gene="gltA"
                     /locus_tag="KLH46_000762"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBE0377565.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45543..46109
                     /locus_tag="KLH46_000763"
     CDS             45543..46109
                     /locus_tag="KLH46_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE0377566.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46160..48619
                     /locus_tag="KLH46_000764"
     CDS             46160..48619
                     /locus_tag="KLH46_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE0377567.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48634..49365
                     /locus_tag="KLH46_000765"
     CDS             48634..49365
                     /locus_tag="KLH46_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBE0377568.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWHENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49362..50409
                     /locus_tag="KLH46_000766"
                     /pseudo
     CDS             49362..50409
                     /locus_tag="KLH46_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50561..51607
                     /locus_tag="KLH46_000767"
     CDS             50561..51607
                     /locus_tag="KLH46_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBE0377569.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51604..52395)
                     /gene="nei"
                     /locus_tag="KLH46_000768"
     CDS             complement(51604..52395)
                     /gene="nei"
                     /locus_tag="KLH46_000768"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBE0377570.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52431..53165)
                     /gene="pxpA"
                     /locus_tag="KLH46_000769"
     CDS             complement(52431..53165)
                     /gene="pxpA"
                     /locus_tag="KLH46_000769"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBE0377571.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53155..54087)
                     /gene="pxpC"
                     /locus_tag="KLH46_000770"
                     /pseudo
     CDS             complement(53155..54087)
                     /gene="pxpC"
                     /locus_tag="KLH46_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54081..54737)
                     /gene="pxpB"
                     /locus_tag="KLH46_000771"
     CDS             complement(54081..54737)
                     /gene="pxpB"
                     /locus_tag="KLH46_000771"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBE0377572.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54760..55503)
                     /gene="ybgI"
                     /locus_tag="KLH46_000772"
     CDS             complement(54760..55503)
                     /gene="ybgI"
                     /locus_tag="KLH46_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBE0377573.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55774..57255
                     /gene="dtpD"
                     /locus_tag="KLH46_000773"
     CDS             55774..57255
                     /gene="dtpD"
                     /locus_tag="KLH46_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBE0377574.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57405..58823)
                     /gene="phrB"
                     /locus_tag="KLH46_000774"
     CDS             complement(57405..58823)
                     /gene="phrB"
                     /locus_tag="KLH46_000774"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBE0377575.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58820..59329)
                     /locus_tag="KLH46_000775"
     CDS             complement(58820..59329)
                     /locus_tag="KLH46_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBE0377576.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59458..59745
                     /locus_tag="KLH46_000776"
     CDS             59458..59745
                     /locus_tag="KLH46_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HBE0377577.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59800..60180)
                     /locus_tag="KLH46_000777"
     CDS             complement(59800..60180)
                     /locus_tag="KLH46_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377578.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60183..64847)
                     /locus_tag="KLH46_000778"
                     /pseudo
     CDS             complement(60183..64847)
                     /locus_tag="KLH46_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(64866..65069)
                     /locus_tag="KLH46_000779"
                     /pseudo
     CDS             complement(64866..65069)
                     /locus_tag="KLH46_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15861 a        17731 c        15975 g        15878 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcatgcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtctgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccacaagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatctcg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gcgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatctatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca aacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt acattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    17461 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
    17521 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
    17581 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggactc
    17641 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg
    17701 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc
    17761 tgagctaatc acccgacact acgctggata ctacaactaa ctatgttagt ggtgggtcgt
    17821 gcaggatgac tcgacttcgt ctcgcccttc ggcgccgttg ctgatgcaac gttatccttc
    17881 acgttttact accgcttcca ccttagcgat tggtgggtcg tgcaggattc gaacctgcga
    17941 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccactt tttcgttgct
    18001 ttcggtttgt ttgatatccc gtggcaacgg cggcatatat tactgatttc agacttgagc
    18061 gcaacaaaaa tttcgatgca gatcactcaa ctgcttatga ttcgcacgac acgaccagaa
    18121 ataatgcgac ttctggtcgt gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg
    18181 tttagcgcca tcagtaccag ggtatttact gataacctgc tggtatacgg ctttcgcttt
    18241 tgcggtgtca cctttgtcct gcatgatgac gccgacttta aacatcgcat cagcagcctt
    18301 tggtgacttc ggatagtttt tcactaccga agcaaaatag tacgccgcat catctttttt
    18361 acccttgttg tagtttaact gacccagcca ataattggcg tttggcaggt aagttgaatc
    18421 agggtaattt ttgatgaaat tctgaaatgc caccattgcg tcatcctggc gggatttatc
    18481 ctgcaccagc gcaatagctg cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc
    18541 gccagcattc gcagtaccag catcagctgt cggcgtcgtt gatgccgtcg caccgctttg
    18601 atcgctgctg gttgattgcg ccgctgcacc accgctgctg aggctgtcga tctgcaacag
    18661 gatctgcttt tgccgctcca cgacctgatt cagttgatac tgattttcct gaatctgacc
    18721 acgcagggaa tcaatatcgg attgattatc agaaagttgt tgctggagtt gggttaaaag
    18781 ctggctgtga gcattagaaa tacgctcaag ttgagtgacg cggtcttcga ccgagcctga
    18841 gccgacacta ctgattggtg cctgagcaaa agcggcccag ggggccgcta taccaaccag
    18901 taacgacaga ctcaatagtt gatgtctgaa gttactgctc atgcaattct cttagtaaac
    18961 cagtaccgca cgacggtttt tggagtatgc cgcttcgtca tgacccagta ctgcaggttt
    19021 ttctttaccg taagaaacga tggagatctg gtctgcagaa acgcctttac cctgcaggta
    19081 catcttaacg gcgttcgcac gacgttcacc cagggagatg ttgtattccg gagtaccacg
    19141 ttcgtccgcg tgaccttcta cggtgacttt gtaagacggg ttgctacgca ggaagtttgc
    19201 atgtgcatcc agcatttgag cgaagtcaga acggatatcg tacttgtcca gatcgaagta
    19261 aacgatgttg ttctgctgca gctgttgcat ttgcagacga gcctgctctt cggaagacat
    19321 gttgccgttg ccgccgttcg catccatacc agtgccggca cccagcatgc cttcgctgcc
    19381 gtcattgctg gcgttcttgt tggaagaaca tgccgcaatt gccataacag gcagagcaat
    19441 catcagccct ttcagcactt tgttcagttg catttcaatg attcctttac tattcaatta
    19501 attattatca cagatacggc gaccaggcag ggaatttgac ctgtccatca gttgccggaa
    19561 gacgcgcttt gaaacgccca tctgtagaaa ccaaattcag cacggatccc atcccctgag
    19621 aagagctgta gattaccata gtgccgttag gtgccagact tggcgtttca tccaggaacg
    19681 tggacgacag aacttgtacg cctcccgttg ccagatcttg tttggcaatg tgctgctgcc
    19741 caccattgga gctgaccatt accataaatt taccgtcgct gctgacatcc gcatcctggt
    19801 tctgcgaacc ttcccaggta atacgttgtg gcgcaccgcc gttgatattc actttataaa
    19861 cctgcggacg accggcctgg tcagaagtaa atgccaggtt ctggctatcc gggaaccagg
    19921 tcggttcggt attgttactg cgaccatcag tcacctggcg gatctgacca gaagccaaat
    19981 ccattacgta caggttcaga ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt
    20041 ctggcgagaa tgcaggtgca ccgttgtgac gcgggaatga agccacctga cgtacagcgc
    20101 catttgccag cgtctgaata accagcgcgg aacgaccgct ttcgaaggtc acataagcca
    20161 gtttagaacc gtctggtgac cacgccggag acatcagcgg ctgcggtgaa cggtgaacga
    20221 caaactggtt gtaaccgtca tagtcagata cgcgcagttc atacgggaac tgaccgccgt
    20281 tggtctgaac aacgtaggca atacgggtac ggaacgcacc tttaatgccg gtcagttttt
    20341 caaacacttc atcactggcg gtatgaccag cataacgcag ccactgcttg ttcactttgt
    20401 acgagttctg agcaagtaca gtacccggtg cgccgccagt gtcaacaagt tgataagcaa
    20461 cattgtaaga accatccgga ttcggagtga cctgaccgac aactacagcg tcaatgccca
    20521 gtgcggacca tgcagctggt tgtacttcct gcgcactacc cggctgctgt ggcagacgag
    20581 cgcgatctaa cggattaaat ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa
    20641 tatcttcagg tgccgcacca ggccccgccc actggaaagg aacaacacca ataggacgac
    20701 cggaatctac accgctgtcg atcacaatgc ggacttcagc atgcagaact gatgcccaca
    20761 gtatgagaaa accaaatgct actcgtaatg cctgcttcat catatctccc ttatctggac
    20821 cgatggccca cgataattta gcagaatgtt aacaaactaa aatacacaaa actaccagaa
    20881 ccccgtggca accggtgcct gatgttgacc gtccgaacag tcaacatcgc gattacggtt
    20941 tgaagtccaa tggcgcgttt ttgaacactt catatactgc ctggcttggt ggtttcggga
    21001 tcttcgcaag tttagctgct gccaacgcag cctgacaaag tgcgggatcg ccaccttcag
    21061 gtttgatatc cagtaacata ccatcgggtg ccagttttat gcgcagcgta caggttttgc
    21121 ctgcatagga cgatgcgtca tagaacttac tttcgatagc agatttaatc tgcccggcat
    21181 agttattgat atcggcccct gatgcgccat tgtttttagt attaccactc ccggcaggcg
    21241 aagcattgtt ccctttcgcc cctccccccg ttttcggtgc attcttacca gagcttagct
    21301 caccgaaaat atcatctgcc tctgcggcag cttttgctgc agcggctttt tcagctgctg
    21361 cttttgccgc tgccgctttt ttgtctgcgg cggctttttc tgccgctgct ttcttatcag
    21421 ctgctgcctt atcagcagcc gctttttctg ctgctttttt gtcggctgca gctttctctg
    21481 ctgccgcttt cttatcagct gcagcctttt cagcagccgc tttcttctca gcttctgcct
    21541 tggctttttc agcagcttca gttgctgctt tctttcttgc ttcagctgca gctgcttctg
    21601 ccgcttccgc tttcttcttc agtgccgcag cggctacctc ggcttttttc tgcgcttcgg
    21661 ctgcggcttt ggcggcttct gcttctgctt ttttcttcgc gtctgcagcc gctttcttcg
    21721 ctgcttcttc cgcagcttta gcatctgccg ccgctttcgc tgccgccact tcagcttgct
    21781 tctgctttaa ctcggcctgt tttgcggctt cttcagcctg ctttttctgc tcctgagccg
    21841 ctaaccgctc tttctcaagt tgcttcaggc gttcctgttc agccgcttgt ttctcacgca
    21901 gttcttcagc agcctgctgt tccttcatct tgcgctgttc atcagaacgc ttcgcgcttg
    21961 attcctggct ttgcatgcgt ttgtactgct caactaccgc acctgaatca accatgacag
    22021 cgtcgatgga cgaaccaccg ccgcctccgg ctgaagcttc tatattctca tcgaacgaac
    22081 tccagatcag cgccgcaaat aagatgacat gcagcactgc tgaaataatt atcgcccgct
    22141 tgagcttgtc gttttgttcg gttgcctttg acactctcgg tttccaaaaa ctgttcgcct
    22201 gttacccgct ctctttcaag caagggaaac gcagatgttt agataggctg cgtcattaaa
    22261 ccaaccgatt tcacacccgc actatgtaac aagttcagtg ctttaattat ttcatcgtaa
    22321 ggcacatctt ttgcgccacc gatcagaaag accgttttcg ggttggcctt gaaacggctg
    22381 gacacttccg ccaccacctg ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc
    22441 acggtgtact gaccaatacc agacacttca acaatcactg gcggattatc gttactgctc
    22501 accgcctgtg attcagtagc gtctggcaga tcgacctcca cgctctgggt gatgatgggc
    22561 gctgtcgcca taaagatcag caacagcacc agcagtacgt ccagcaacgg aacaatgttg
    22621 atttcggact tgagatcgcg acgacctcgt ccacgcgctc tggccatggc ttaccccttg
    22681 ttgctctcgc taacggtaaa cgcctggcgg tgcagaatcg cggtaaactc ttccataaag
    22741 ttgtcgtaat tcagttccag tttgtttacg cgctggttga gacggttata agccattacc
    22801 gccggaattg cggcgaacag accaattgcc gtcgcaatca acgcttctgc gataccgggc
    22861 gcaaccattt gcagtgttgc ttgttttacc gccccgaggg cgataaaggc gtgcatgatc
    22921 ccccagaccg taccaaacag accaatatac gggctgatgg agccaaccgt accgaggaac
    22981 ggaatgtgcg tttccagatt ttcaagttca cggttcatgg agatacgcat agcacgcgac
    23041 gccccttcca ctacggcttc cggcgcatgg ctattggcac gatgcaggcg cacaaactct
    23101 ttgaacccgc tgtaaaagat ttgttccgaa ccagtcagat tatcccgttt cccctgactc
    23161 tcttgataga ggcgagagag ttcgattcca gaccagaatt tatcttcaaa cgcttcggct
    23221 tcacgcgccg ctgcgttaag aatacgggtt cgctggataa taatggccca ggatgcgatt
    23281 gaaaaaccaa tcaaaatcaa catgataagt ttaaccagaa ggctagcctt caggaacaaa
    23341 tcaaggatat tcatgtcagt cactgcttaa actccgcgac aatagacttg ggaagcgcac
    23401 gaggcttcat tttgagtggg tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag
    23461 tattctcggc gttgacaata cgttgcgtga aaaccaaaga ggtgccacgc attgatgtta
    23521 tttcagtctg tatttcgagc atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca
    23581 ttttacgtac cacaaaggca acgcgttcag ccatcagcgc ctgctgactg aagtgatgat
    23641 gacgcagcat ctctgtgcgt gctctttcat aaaaagcgac gtaactggcg tggtacacca
    23701 caccaccggc atcggtatct tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat
    23761 tcactttaca tcccggtaat gcaacaaaag ttagagcttt taaacgctgc tactatacgc
    23821 gcgcgattga ggtttgggaa tgagggcaag ttaagggcgc gtaaaaattt atgggcctgg
    23881 agcggcccat aattttttaa agagaattct gatttaaaag aagaagaaaa tcagcccgac
    23941 aatgagaaca atatcggcaa gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg
    24001 acgaaagccc acgccgtgaa tcacaccagc acagacggcc cacatcagca ataaaccatg
    24061 ccagatttcc agttcactgg tcttcgcggc aaaacgagat gggtcccaaa acatacatcc
    24121 tgctaacaga agcgccatca cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc
    24181 atacaaggtc gcgataatct tactcatcag atgtcttctt gaccggcttt gcctgattcg
    24241 acgtgttcaa gcgccagcgc ggtgattacc ccaaacgaac aggcaagaag cgttcccaga
    24301 atccatgcga aataccacat ttttagctcc ttacttagta cagagagtgg gtgttacgtt
    24361 caatatcttc tttggtgata cgaccgaaca ttttccagta acaccaggcg gtgtagagca
    24421 gaatgatcgg taccagaacc accgcaaccc aggtcatgac gttaagcgtc agctggctgg
    24481 aagttgcatc ccacattgtc agacttgcgt tcatcatggt gctggacggc atcacaaacg
    24541 ggaacattgc gataccggct gtcaggatga tgcaggccag cgtcagggag gagaacacaa
    24601 acgcccacgc ggctttatcc atacgtgcag tcaggatggt cagcagcagc agaaccacac
    24661 ccagtgccgg aatagcccac agaattggcg tgttgttgaa gttaaccagc catgcgccag
    24721 cttcacgaac cacttcttta ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt
    24781 tcacgacata accatcgata ccgtacatca cccatacgcc agccagtgcg aaacagacca
    24841 gtgtcaccag cgcagccacc tgagccgttg cacgggtacg caggtgcagt tcgcccacgg
    24901 tacgcatttg cagataggtt gcgccctgag taatgatcat ccctacgctc accacgcctg
    24961 ccagcaggcc gaacgggtta agcaactgga agaagttacc ggtgtagtac agacgcagat
    25021 attcatcaac gttgaacggt acgccctgca acaggttacc gaacgctaca ccaattacca
    25081 gcggcggaac gaagctacca atgaagatgc cccagtccca catgttacgc cagcgggttt
    25141 cttcaatctt ggagcggtag tcaaaaccga ccggacggaa gaacaaagac gccagcacga
    25201 ggatcatcgc cacatagaag ccggagaacg cagcggcata gaccatcggc caggcagcaa
    25261 agagtgcgcc gcccgcggtg atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg
    25321 agttaatcat aattcgacgc tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca
    25381 cccccatgtc gaaaccgtca gtgactgcaa aaccaatcag cagaacgcca accagcagcc
    25441 accagataaa acgcaatact tcataatcga tcatttgacg actcctgtct tagcgtgccg
    25501 gctgagtagt cgtggaagac tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc
    25561 cgaggcgtgc aaacttgaac attaagaaca attctgccac caggaacagg gtatacaggc
    25621 cgcaaatcag caccattgag aagatgagat cgcctgcggt cagtgacgaa ttcgccacag
    25681 ctgtcggcag cacttcaccg atagcccacg gttggcggcc atattcagcc acgaaccagc
    25741 ccgcttctac agcaatccac ggcagcggaa taccgtacag cgcggcgcgc agaagccatt
    25801 ttttctcgcc aatgcggttg cggatgacac tccagaaaga gagcgcgatg attgccagca
    25861 gcaggaagcc acacgccacc atgatacgga acgcaaagta cagcggcgct acacgcggga
    25921 tggagtcttt ggttgcctgt tgaatctgcg cttcagtcgc atcagccacg tttggcgtat
    25981 agcgtttcag cagcagaccg taaccgaggt ctttcttcat gctattgaac tggtcacgaa
    26041 ccgcctggtc ggtagaacca gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt
    26101 tacgaatgcg ttcttcatgc tgcaccatca gctctttcag gccgataacc ggggtatcca
    26161 cggaacgcgt tgcaatgatg cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct
    26221 cctcttcctg atcaggaatg ccgaacaaag taaaggcagc aggcgcaggt tgcgtttccc
    26281 actcggcttc aatagcagcc agtttggttt tctgcacgtc gcccatttcg tagccggatt
    26341 catcacccag aacaataaca gacagaacag cagccatacc gaagctggca gcgatagcaa
    26401 aggagcgttt agcgaaggcg aagtcacgac ctttcagcat ataccatgcg ctgataccga
    26461 ggatgaacat cgcgccagtc acataaccag acgctacagt gtgaacgaat ttcacctgag
    26521 caaccgggtt aagcaccagc tcggagaagc tcaccatctc catacgcata gtttcaaagt
    26581 tgaaatcgga cgcgattggg ttttgcatcc agccgttcgc aaccagaatc cacagtgcgg
    26641 acaggtttga accgagcgcc accagccagg tgacacacat atgctgaact ttacccagac
    26701 gatcccaacc gaagaagaac agacctacaa aggtggattc gaggaagaag gccatcagac
    26761 cttcgattgc cagcggcgca ccgaagatat cccctacata gtgggaatag taagaccagt
    26821 tagtcccgaa ctggaactcc atggtcagac cggtagccac acccagagcg aagttgatac
    26881 caaacaactt gccccagaac ttggtcatat ctttataaat ctgtttgccg gagaggacgt
    26941 agaccgtttc cataatggcc aggaggaacg ccataccgag cgtcagtggc acaaaaagga
    27001 agtggtacat cgcggtcaag gcaaactgta agcgcgacag ttcgactata tctaacatca
    27061 tgactccttg ctcatcgcat gaagactccg agagtgaacc ccgttagaaa gggtcacacg
    27121 catgccccaa tagaaatttg tttgaacact ttcatcatac catgtgaatg cacacaaaaa
    27181 cggcgtaaag aaaggttgca aacatgttaa taaaaactca aattgatccc acgtatatat
    27241 tacgccgcaa aatccttaca ataaacaggt ttttgttgaa gcaaatttgc atttttcgac
    27301 ggtgatcaat ttatagctaa attgccgcct ttcagccaat ttgatcgaga acaatttatc
    27361 tctttttgat gcccatttcc aagacttata cattgataaa tatcaatttc tacaaattat
    27421 gttaatcatg tttcaacaag gtaactttag tgatatttat gtttaaaatg tgtgaattat
    27481 aagactgaaa ttagtttcgg ttggcaatta aagataaaaa gagacgaagc aaaaacagca
    27541 cggttgacct taattctttt tttggtcact ggctaatacg acgcagagtt tcaggattat
    27601 taataaaggc ataaatctcc cccccgccga tattaacatt taattaactt ataattatat
    27661 tataattaac tcacccgtgg ttattatttt gttaaaaata aagccacata tacgtagctt
    27721 gagagtgaag ataaacacaa agttacctga tgcgctacgc ttgccagtgt ctacatcatc
    27781 tctgcaatat attgaatttg cctgcttttg tagaccgaat aaagcgttca tgccgtatcc
    27841 ggcatgaaca aagcgccctt tgtcaacaat ctggccgcgc atatgtggct tcggtttgat
    27901 tgctattcag gcaagccggt aactgaacgt ccgtgactgg ccgggtaaaa aaggccccga
    27961 tagatttgaa ccttgatttt cctcggtggt aatgtgttca tccatcatcg tttctgagca
    28021 agaaatcacc tcgcgactga aagcaacagt cgcatcacag gttgctgatt cagccggatt
    28081 aaacagccgt aaaatcactt cttgtcggtc ttcagctttc ttaagtgcgc ttatcaggca
    28141 tcccactggg ggcattttca acaaactata actttccggc acgttgaatc cggctttgtt
    28201 gagcttcatc gcatcccatg ggattttgtt gtaacactgt actggagtca gccatgctcg
    28261 cgcctgctga gctacaccag cggcggttgg cgtaccggta taactcaata aacttaggcg
    28321 acaagaaagc agaccacgta gttgtgagtc cgggactggc attttaattc ccgaaggccg
    28381 cctaagcctt aaaagcagat cttctttccc cagtaagccc acgccgcgca gcaacgtaat
    28441 ggcaaaggtt ttcttctctt caccgatgac ttcaaattca cgtagccctt cgctaaagac
    28501 agccatgccg ttacgccctt cctgtaaggc tgcatagttg agcatattcc ataccggaac
    28561 cggcgcttct ttccagcctt cttgctgcca gttgttcatt gcactgtcgt tcacggggcg
    28621 cgttagcgaa ccaaactggg tatctgccag aacactatcg gtgttaaaag atgtagggat
    28681 caggacgcga aggcgatgat cgtcagcctg gttatcaagg ttgatatcca catcaatgcg
    28741 cctgttgtta tgactaagag tgacaaccaa ctcgacatct actctgccag tggattgtct
    28801 tgcgctgcgt tgttgcagat tacgcggcac cgccatttca tagcggataa cagccctgct
    28861 ctgccaggcc tcatgaataa tctcgcattg cggtttcgca ttcgccgaag taattaccca
    28921 ctcttctttt gcgggtgaat agtcatattc atcaccatca tcagagcttt cttcaatttg
    28981 caacacccgg tcatagcgca caccgctatc tttatctacc agttgtagcg ttccatcctc
    29041 attgagcgca atttgccaga aagcattttc cagtatccct tcagcgtcac tttttgccgc
    29101 aatcacgttg ccaggctgat tcgcttcgat ataaagcgtg cgatagccca tagaagggac
    29161 aatctggttg atctgtatat caaactccat aaagggatcg taattaccgt aatgaactat
    29221 ttgccgatcg attaggcctg gatcgatctc acgggcatgg cgaataaaat acggtacagg
    29281 ctgaccgcga tcgtctcgta gattaaactg gctggcgcgc agccgcacag tggtgttgat
    29341 cacttcttcc cgcggccagg gcatcaggtt aaacaggacg agtttgtcgg cgtcgctctg
    29401 cggcatgttg tcggcgattt tacgcatata gaaacgcagc agattatccg ccatgtcttc
    29461 agccagttcg aagcgggcga cgatttcgcg atgaactttg tcactacagc agcagccgat
    29521 gctgtcgtgg gcatgatttt ttaagatctc tttccacatt ttctccagca agccgtggtg
    29581 gtatccaaaa cccaacgtcc tggccagcgt tgccagcggt tccagcagat taacaatctt
    29641 attttcaata cgtgcgtggg caactttgat atccatgcgc gtggaaccga tggtgcgatg
    29701 cacgcgcata tatttgccat caataaattc ccctttcagg gttgccagat tatctcgctg
    29761 cgcttcgatc ttctcaaata cctcttcaaa gcggctcatc acaaactttc gttgaggata
    29821 aatttcgcgc agtttctcca tcacttcgaa gatattttgc tgcaatggca tctgatcatg
    29881 cccattcggc agcaaaatct ctttggttac agacgctttt tccagcacgt caaaataact
    29941 gtcgaggcgt ttgcgtaatc cgttttcgtc cgcaggtaag tacttaccga tggcataacc
    30001 caacggcatc acctgcgccg tcacttcgct accgtcactg ctttgccaca aaaactcggt
    30061 tttatccgta ccgtggcgct cagaacatcc acgccagaac atggtgcagg taatgccaaa
    30121 tccattgtag atatgcggca gttgcccgga catgccaaag gaatcgggta aataacctat
    30181 tttcatcggc tcgccaaacg cgaggcagtc acgcattccg tacatcagat tacggacgat
    30241 ggattccgca gaaacaatcg tggtatcggt ctgggtatac taggggccga taatcaactt
    30301 gccggcttct accagtttct tcacgcggtc tttgttttcc ggtttcaccg cgaaataatc
    30361 ttcgaggatc gccgtttgcc cgtcgagtac gtaatatttg tattcgttgt cctgttccag
    30421 tcggcacagg atctcttcca tattattgac cagcagaatg cgtgactctt cggtggtgaa
    30481 ataccactcc cgatcccagt gcatatgcgg ggtgatgtga acgcgagata ccgctttcat
    30541 cgtcgtttcc tttttgttta tggcattacg ccatcagtca gatagttgcc atgcttaacc
    30601 gcgtgacgcc gccagatcag gagaattgca gtcgagattg cagcccccac caatgccgca
    30661 ccaaaccagc caattgccgc cataacaccg cccgcgccat tatcatgaag taaaaagagc
    30721 gagaaaatgc cagcaccggg tgtcgaaagg ccgatattca tcgcaccgac aatagcgccc
    30781 gttaccatag agcccagcac aaacgaacca ataacccgca gcggatcttc aatcgccatc
    30841 gggatcgccc cttcggtaat acctgccagc cctaacagcc aggtggattt cccggtctca
    30901 atttcaaact ctttaaacag gcgcggtgcg agcatcgtgg aagcggttac ggtaaatgcc
    30961 gaaaccattt tgacggaggc gaaaatggca tacgggccgt aaacgccgtt cgccattgcg
    31021 cccaggcaga atgcataagc ggctttattc actggccctc caaggtcaaa ggaacacata
    31081 aaaccgagaa tggcacccag caacagcgcg ttacttcctg acagaccgtt cagccaggcg
    31141 gtaagcgagt tattgatcca ggcgacaggt tcccccacca caaacagcat cagactgccc
    31201 gctcccaatg taccgagcac cgggtagaga taaaaagtca ggaagccatt gaatttactg
    31261 ctaagacgca agtgattttt catccagcgt atcaagtaac cggctatcaa tccgccaacg
    31321 accgcgccga gaaacccgga gccgatcatg ttggcggcaa gtccagccgc aaagcctggc
    31381 gctaacgccg gtttatctgc cagcgaatag gcggtatagg ctgccagcac cggtaccatc
    31441 aaaattccga gcagcccgcc gcccagtttg cggtacatcc acagccagga gttttcttca
    31501 ttaaacagat cttgtagccc gaagatttgc gacagtaata ccgcgaccgc cagcaccgtc
    31561 ccccccgcga caatcaacgg tacggcaaaa gagatcccgc tcaacagtgc ctgtttcagc
    31621 tccgttttga cgcttttcac cggttgcgta tcttgttgta aggtacgcgt ctcatcgcca
    31681 cgctcgaggg taagcgattg ttggatcaac gcttctgcat ggcgaatcgg ctcggcaaca
    31741 ggcacagaaa gcgcaggaat gccgttaaaa cgctcactct ccttgatggc gacttcagcc
    31801 gcaaaaatac aggcagttgc actattgagt tgatccgccg ttaaacgccc ttcaataccg
    31861 ttagcacctt gcttttcaac gtagacattc acgccgagtt tgcgtccggc tttttccaga
    31921 tactccgcag ccatgtaggt gtgagcaata cccgccgggc aggccgttac gcaaacgata
    31981 gttggcgcgt tggaaaaaga ggcagaaggt tgcgtgcctc ccttgtcatc cagcgccgag
    32041 agcaattcat caggcgtcgt cgccgactgt atacgcgccc gaatctcatc atccgccagg
    32101 cgcgtggtca gcgctgtcag cagttgcatg tgcgtagtac ccgcttcatt gaggggaatc
    32161 gccagcaaca ccactaaatc aaccgcttcc gggccatcca cgccttccca ctgaagcggc
    32221 tcgctgagtg tggcgaccgc aaacgccgct tcttttaccg cagcagtttt gccatgcggc
    32281 acagccaacc cttcacttaa ggcggtcggg ccaaggcttt cacggcgata cacttctttc
    32341 agaaattgct cagtactgga aattttcccc agagcagcaa gacgttgagt caacgcgtga
    32401 atggcctctt cacggctggt aaagcgcgca ttcagacaca acgcatcgcg gtgggtcaga
    32461 gtcgtcaggt tcatcatgat cctcacttga tcgtttttat agtcgcgcca gtgtgcccga
    32521 caaaacaata ccattgcgta atcaatttca cttgattgaa attatttgca ttttatttgt
    32581 attgtttaca ggatgaattt atcgggcata atggctaaat tcgtattaat gagatgctaa
    32641 ataatgggac acaaaccctt ataccgacag attgctgatc gcattcgtga gcaaattgcg
    32701 cgtggcgagc ttaaacctgg cgatgcgtta cctaccgaat cggcattaca aacagagttt
    32761 ggcgtcagcc gggttacggt gcgtcaggct ttgcgccagt tagtcgagca acagatcctc
    32821 gaaagcattc agggcagcgg gacttacgtc aaagaagagc gggtcaatta cgatattttt
    32881 cagttaacca gttttgatga aaaactgtcg gaccgtcacg tcgatacaca cagtgaagtt
    32941 ctgatatttg aagtgattcc ggctgacgat tttcttcagc aacagctaca aatcaccgtg
    33001 caggatcgtg tgtggcatgt gaagcgcgtg cgctatcgca agcaaaagcc aatggcgctg
    33061 gaagaaacct ggatgccgct tgcgttgttc ccggatctca cctggcaggt catggaaaat
    33121 tcgaaatatc actttattga agaagtgaag aagatggtta tcgatcgtag cgaacaggaa
    33181 atcattcctc tgatgccaac cgaagagatg agccgtctgc ttaatatcag ccagacaaag
    33241 ccgattcttg aaaaagtatc gcgtggatat ttagttgatg gtcgggtgtt tgaatatagc
    33301 cgcaacgctt ttaataccga tgactataaa tttaccctga tagcgcagcg aaaatcatcg
    33361 cgataagcac aaaaaggcca tcatacgatg gccaaccatg tcgaaaccga cagcccttcg
    33421 ccattcctgt agagggaagc ggcgagggct atttcttatt acagatattt atttcagaac
    33481 agttttcagt gcttcaccga tatccgccag gctgcgaacg gttttcacgc ctgcggcttc
    33541 cagagcagcg aatttctcat ccgcagtccc tttcccaccg gcaatgatcg cacccgcgtg
    33601 gcccatacgt ttgcctttcg gcgcagtcac accagcgatg taacccacaa ctggcttggt
    33661 aacatgctct ttgatgtacg ctgccgcttc ttcttcagcg ctaccgccga tctcaccgat
    33721 catcacgatc gcttcggtct gcggatcttt ttcgaacatt tcgagaatgt cgataaagtt
    33781 agagccaggg atcgggtcac cgccgatacc gacacaggtc gactgaccga aaccgtaatc
    33841 cgtggtctgt ttaaccgctt cataggtcag tgtaccggaa cgggaaacga tacccacttt
    33901 acccggtttg tgaatgtgac ccggctggat accaattttg cattcacccg gggtgataac
    33961 gcctgggcag ttcgggccga tcatacgaac gcctgcttcg tccagtttca ctttcacggt
    34021 cagcatatcc agcgtcggga tgccttcagt gatggtgata atcagtttga tgcctgcatc
    34081 gatagcttcc agaatggagt ctttgcagaa cggtgctggt acgtaaataa cagacgcgga
    34141 agcgccagta gcagcaacag cttcacgcac ggtgttgaac accggcaggc cgaggtgggt
    34201 agtgccgcct ttacctggag ttacgccacc aaccatttta gtgccgtatg caatggcctg
    34261 ttctgagtgg aaagtcccct ggctaccggt aaagccctgg cagataacct tggtgttttt
    34321 atcaattaaa atggacatta tttcccctcc actgcggcaa caacctgctg agctgcatcc
    34381 gtcagacctt ttgctgcaat aatattcagg ccgctgtcag ccagtttctt cgcgccgagt
    34441 tcggcgttgt taccttccag acgtaccacg accggtacgt taacacccac ttccgctacc
    34501 gcgccgatga taccgtcagc gatcaggtcg caacgaacga taccgccgaa gatgttaacc
    34561 agaacggctt tcactttgtc gtcagagagg atgattttga acgcttcggt tacacgttct
    34621 ttggttgcgc cgccgccaac gtccaggaag ttagccggtt cgccgccgtg cagtttaact
    34681 atgtccatcg tacccatcgc cagacctgcg ccgttaacca tacaaccgat gttaccgtcc
    34741 agcgcaacgt agttcagttc ccactgtgca gcctgtgctt cacgcggatc ttcctgcgac
    34801 tggtcacgca tttcgcgcag atcaggctgg cggaacaggg cgttgccatc agcgcccagt
    34861 ttgccgtcga ggcaaatcag atcgccctgt ttggtgatga ccagcgggtt gatttcgatc
    34921 agcgccaggt cgcgctccag gaaaatggtc gccaggccca tgaagatttt ggtgaactgc
    34981 tgaaccagtt taccttccag acccagtttg aacgccagct cgcgtccctg atacggcatc
    35041 gggccagtca gcggatcaag cgcaacttta tggatcaggt gcggagtttc ttccgccact
    35101 ttttcgattt ccacgccgcc ttcggtggag gccataaaga ccacgcgacg ggaactacgg
    35161 tctacaacgg caccgagata cagctcttta gcgatatcgg tcgccgcttc aaccagaatc
    35221 tggttaaccg gttggccatt ggcatctgtt tgatacgtta ccagacgctt gcccagccag
    35281 ttttctgcaa aagcacggat gtcttctttg ctgtttacaa ctttcacacc gcccgcttta
    35341 ccgcggccac cagcgtgaac ctgacatttc actacccacg gaccggcacc gatttttgaa
    35401 gcggcttctt ctgcttcgcg cggagtagta caggcataac ccaccggtgc tggtaagcca
    35461 tagcgggcaa acagttgttt tgcctgatat tcatgtaagt tcatgtgttc tgtccatcct
    35521 tcagtaatcg ttatctttta aaccgtagac cgacacccta tccacgtcgg tctacagtgc
    35581 aggtgaagct taaactacta cacgtccagc agcagacgcg tcggctcttc cagtaactct
    35641 ttgatcgtta ccaggaagcc cacggattcg cgaccatcga tcagacggtg atcgtaggac
    35701 agcgccaggt acatcatcgg caggatctca acctgaccat tcaccgccat cggacgatct
    35761 ttgatagcgt gcatacccag aattgcgctc tgcggcgggt tgatgatcgg cgtagacatc
    35821 agggaaccga acacaccacc gttggtgatg gtgaagttac caccggtcag atcttcaacc
    35881 gtcagcttgc catcacggcc tttgactgcc agctctttga ttttcttctc gatgtctgcc
    35941 atgccgaggg tatcgacatc acgcagaacc ggcgtcacca ggccgcgcgg cgtagaaacc
    36001 gccatgctga cgtcgaaata gttgtggtaa accacgtcat cgccgtcgat agacgcgttc
    36061 acttccgggt aacgtttcag ggcttcaacc accgctttca cgtagaagga cataaagccc
    36121 agacggatgc cgtggcgttt ttcaaacgct tcaccgtact gcttacgcag atccataatc
    36181 ggcttcatgt tgacttcgtt gaacgtggtc agcatggcgg tggagttttt cgcttccagc
    36241 agacgctctg ccacacgctt acgcaggcga gtcatcggga cacgtttttc actacgtgca
    36301 gccagagccg gttgcgccgc cggagcagcc gctgccggag cagactcttt cgccggggct
    36361 ttcgccagat gtttttccac atcttcacga gtcagacgac cacccacacc ggtgccttta
    36421 atggcgctgg cgtcgagatt gtgttcagcc agcaggcgac ggatcgccgg gcttaacgca
    36481 tcgttgtttt gctcttccag agacgcctgc tggcgttgcg ccggagtgga cgctttctct
    36541 tcagatttgg cgctggtttc tttaccggcg ctgttgcctt cacgcaggcg accaaggatc
    36601 tgacgagacg ttaccgttgt accttcatct tccagaaccg catccagaat gccgtctgct
    36661 gatgccggta cttccagtac cactttgtca gtttcgattt ctaccagcac ttcatcacgt
    36721 acgactgcgt cgccgggttt tttatgccag gttgcgacgg tggcatcggc tacggattca
    36781 ggcaggtcag ggaccagaat atctacgcta ctcattgtgt atcctttatt tattcgacgt
    36841 tcagcgcgtc attaaccaga tcttgttgct gtttctggtg aacggacata taccctaccg
    36901 ccggagaggc ggaggccggg cggcctgcat aacgcagaga agccccaaac ggaatcactt
    36961 cacggaaatg atgctggctg cagtaccatg cgccctggtt gagcggctct tcctggcacc
    37021 agacaaaatc cttgacgtga gcaaactgct gcaacacttc ctgcatcgct ttatgcggga
    37081 acgggtagag ttgctcgata cgcacaatgg cgacatcgtg ttgattgttc ttacgacgct
    37141 gttccagcag gtcgtaataa accttaccag aacacatcac tacgcgcttc acgcccttcg
    37201 gatcaagctc gtcgatttca ccgatggctg gcaggaaggt gccgttcgcc agttcttcga
    37261 ggctggaaac cgccagcgga tgacgcagca gggatttcgg cgacatcacg accagcggac
    37321 gacgcatccc gcgcagcgcc tgacgacgca gcatgtggta aacctgtgcc ggggtagacg
    37381 gtacgcaaac ctgcatgttt tgctcagcac aaagttgcag ataacgttcc agacgcgcgg
    37441 aggagtgctc cggcccctgc ccttcgtaac cgtgcggcag caacatcacc agaccacaca
    37501 tccggcccca tttctgctcg ccagaggaga tgaactggtc gataaccacc tgcgcaccgt
    37561 tggcgaagtc accgaactgc gcttcccaga tggtcagggt gcgtggttct gcggtggcat
    37621 aaccatattc aaacgccagc acggcttctt ctgacagtac ggagtcccag acacggaacg
    37681 cgccctgacc gttatggatg tgttgcagcg gcgtgtaagt ggaaccgtta gactggttgt
    37741 ggatcaccgc gtggcggtgg aagaaggtac cgcgaccgga gtcttcaccc gacaggcgaa
    37801 ccggaatgcc ttcatcaacc agcgtggcgt aagcgaggtt ttccgcaccg ccccagtcga
    37861 acagtttctc accggctgcc atcgcctggc gatcgccata aatcttggca acgcgagact
    37921 gcatttcaac tgcttctggt accgtactga tacgtttagc cagttcctgc aggcgcttca
    37981 tctcaacttt gttcgggtac tcttcgtccc attcgtggtt gaggtacggc gaccaggtga
    38041 aagagtgcat gttcatcgga cgccactctg ccactacgca atcgccagca tccagcgcat
    38101 cgcggtacag gttaaccatc tcggtggcat cttccagcgt cgccactttt tcctgctcca
    38161 gcttgtcagc gtagattttg cgcggtgtcg gatgtttttt gattttctga tacatcagtg
    38221 gctgggttgc gctcggctcg tcggcttcgt tgtggccgtg acggcggtag cataccaggt
    38281 cgatgaagac atcacgttta aaggtgttac ggaaatcgag cgccagacgg gtcacaaagg
    38341 caacggcttc cggatcatcc gcgttaacgt ggaaaatcgg tgcctgaacc atcttaccga
    38401 tatcagtaca gtacggtgta gaacgggcat ccagcgggtt agaggtggtg aaaccaacct
    38461 ggttgttgat aacgatacgt accgtaccgc caacttcata accacgcgct ttcgacatgt
    38521 tcagagtttc ctgaaccacg ccctgcccgg tcactgcggc gtcaccgtgg atggtgattg
    38581 gcagcacttt gttgctgttc ggctcatcaa gtctgtccag acgggcacga acagagccga
    38641 taacgaccgg gcttacaatc tcaaggtgag acgggttaaa cgccagcgcc aggtgcacca
    38701 ggccgccatc ggtctggaag tcagacgaga agcccatgtg gtatttcacg tcgcctgtgc
    38761 cgaggtgttc tttatgttta ccggcgaact cgtcgaacaa gtcttgcggt tttttaccca
    38821 gcacgttcac cagcacgttc agacgaccac ggtgcgccat cccgagaacc acttcgcggg
    38881 tgccgctgtt gccagcgtgg cggatcatct ctttaagcat cgggattaac gcgtcaccgc
    38941 cttccagcga gaagcgtttt gcgccaggga attttgcgcc gaggtaacgc tcaaggcctt
    39001 cagcggcggt cagttcgctt aagaagcgtt ttttctcttc gctattgaaa gtcgcgcgac
    39061 cagactcgat acgctgttgg atccagcgtt tttcttcggt gctggtgatg tgcatatact
    39121 cggcaccaat cgggccgcag taggtttgct tgagggcgga aataagctcg ccgagtttca
    39181 tggtttcttt gccgctggca aatgaaccga cgttgaaggt ttcctggaag tctgcttcgg
    39241 tcagatcgtg gaaagacgga tccagatcgg ccactttatc ttgctgccac agtcccagcg
    39301 gatctagatt cgcatgctgg tgaccacgga agcggtatgc gttaatgagc tgcaggactt
    39361 taacctgctt cacattggtg tcagggtcgg agatcgttga agagtaacgt gaagcgtctt
    39421 tcgccaggcg gcggaaatat tcacgcgttt gagagtggaa ttgatccggt ttgactcccg
    39481 taccaggtaa ctgctggaac gtcgaacgcc agttagcgtc aaccgagtca ggatcggtta
    39541 agaagtcttc atagagctgt tctatccagc tctggtttgc gccagagagg taagaagagt
    39601 ccaaccaggc tttcaaagcg ctgttctgca tcgtgatccc ttaagcatct tttttatgct
    39661 tacttcgccg tggatactac cacgcacagt gatgtgcgtg ccggggttca cttgcgagct
    39721 cttgtctacg tagtggctcg cgaaggaacc tttaaaaact gtcttagtgt caggggcagt
    39781 ttttaaagat ttcctgcatt tctttgttac gcctgatgcg ctgcgcttat caggcctgcg
    39841 ggtaatgact tgtaggccgg ataaggcgtt cacgccgcat ccggcactgg ttgcctgatg
    39901 caacgcttgc gcgtcttatc aggcctacag tttacgcatt acgttgcaac aacatcgact
    39961 tgatatggcc gatggcgcgc gtcgggttca gccccttcgg acatacactg acgcagttca
    40021 tgatgctgtg acagcggaat acgctgaatg catcgctcaa accgtcgagg cggctgtcag
    40081 tctcggtatc acggctatcg atcaggaaac gatatgccgc taacaagcct gccgggccga
    40141 taaacttatc gggattccac cagaaagacg gacaagaggt tgaacaacat gcgcagagaa
    40201 tacattcata cagcccgtcg agtttttcgc gctgctctgg catctgtaaa tgctcgcgag
    40261 ctggcggatt ttgtccatta ttcaacaggt aaggcttaat tttctcatat tgcgcataga
    40321 attgtcccat gtctaccacc aaatcgcgga tcaccggtaa acctggcagc gggcgaatca
    40381 caatcttctt gcccggctgg ttgagtgccg aaatcggggt aatacaggcc agaccattct
    40441 tgccgttcat gttcagaccg tcggaaccgc acacaccttc acggcaggag cggcggaacg
    40501 acaggctggg atctttctct ttcagctgga taagcgcatc cagcagcatc atgtcgcgac
    40561 cttcttccgc ttccagggtg taatcctgca tacgcggagc atcatcaaca tccgggttat
    40621 agcgataaat tgaaaactcg agtctcattt tcctgtctcc gcattagtaa gtacgaatct
    40681 tcggcgggaa tgccgggcgc agtttcggtt ccatgttgac gcttcggcgc gtcatggatt
    40741 ccgactcagg cagatacagg gagtggcaca gccagttttc atcatcacga tccgggaagt
    40801 cgaagcggct atgtgcgcca cggctttcgg tacggaagtt ggcagaaaca gccgttgcat
    40861 acgccgtttc catcaggtta tccagttcca ggcactcaac gcgctgggtg ttgaactcgc
    40921 tggaagtgtc atccagacgg gcatttttca gacgctcgcg gatcactttc aactgctcaa
    40981 gccctttcgc catcgcatca ccttcacgga agaccgagaa gttatgctgc atacattctt
    41041 gcagcgcttt acggatcgcc accggatctt caccgttacg attattgttc caccggttca
    41101 ggcgatccag agacgcttca acatcagact cgctggcatc gtgcagtgcg ccctgctcgg
    41161 cgatagactc ttgcagatgc agacctgccg cgcgaccaaa gaccaccagg tccagcagcg
    41221 agttgccgcc cagacggtta gcgccgtgta ccgatacaca agcgatttca ccaacggcaa
    41281 acagtcccgg aacaaccaca tcttcgcctt tctcattcac agtcagcgcc tgaccggtaa
    41341 ctttggtcgg aataccgccc atcatgtagt gacaggttgg gataaccgga atcggctctt
    41401 tcaccggatc gacgtgagcg aaggtacggg aaagctccag gatacccggc agacgggatt
    41461 cgagaacttc tttacccagg tgatcgagtt tcagtttcgc gtgtggcccc cacggaccat
    41521 cacagccgcg accttcacgg atttcaatca tgatggaacg cgccaccaca tcacggcccg
    41581 ccaggtcttt ggcgttcggc gcataacgct ccataaagcg ttcgccatgt ttgttcagca
    41641 gataaccgcc ttcaccacgg caaccttcgg tgaccagtac gcccgcaccg gcaatgccgg
    41701 tcgggtggaa ttgccacatt tccatatcct gcaccggtac gcctgcgcgg atagccatgc
    41761 cgacaccgtc gccagtgtta atgtgggcgt tagtggtgga ctgataaata cgccctgctc
    41821 cgccagtcgc cagcacggta gcgcgggctt tgaaataaac cacttcaccg gtttcgatgc
    41881 acagtgcggt acaacccacc accgcgccat cctggttttt caccagatcc agcgcatacc
    41941 actcggagaa aatggtggtg tggtttttca ggttctgctg ataaagcgtg tgcaacagtg
    42001 cgtgaccggt acggtcagca gccgccgcag tgcgtgccgc ctgctcgccg ccgaagtttt
    42061 tcgactgacc gccaaacgga cgttgataga tacgaccatc atcgagacgc gagaacggca
    42121 ggcccatgtg ttcgagttcc agaatcgctt ccggcccggt tttacacata tattcaatcg
    42181 cgtcctggtc accgatatag tccgaccctt ttacggtgtc gtacatatgc cattcccagt
    42241 tatcttcatg ggtattaccc agcgcaacgg taatgccgcc ttgcgcagaa acggtatggg
    42301 aacgggtcgg gaagacttta gagagcaggg cacaggtctg gccgctctgg gaaatttgca
    42361 gcgccgcgcg catacctgcg ccaccggcac caatcacaac tgcatcaaat tctctgactg
    42421 gcaatttcat cacacacccc acaccacaac gaatccataa atcacgtaaa ccaccagtgc
    42481 aacgacaatc accagttgca gcatcaggcg caaggccagc ggtttaacgt agtcggtcaa
    42541 cacctgccac atgccgatcc aggcatgaat caagatagaa aacagcgcca gcagggtgaa
    42601 cactttggtg aacgcagagg cgaagaaacc gatccagact tcatatgtca gttcgccact
    42661 ggtggcgaaa aaaccgacca tataaatgat gtagagcgtc aggacgatag cggtagcgcg
    42721 aacgaggatg aaatcatgta cgccattgcg tcctaatgcg gaggcgttgc ttaccatacg
    42781 aggactcctg cgagaagtga aagcacgaca gtaataacaa aggagatttt ggcggagcgt
    42841 ttacccgctt cgaatgtttc ttccagatag ccaaaatcca tcatcatgtg gcgaatacct
    42901 acgacgacgt gatacgccag agcggtaagg atgccccaca tgataaattt gacgaagaag
    42961 ctgcccataa tcgcggaagc ttgctcgaaa ccttcagggg aagagaggct ggtacccaga
    43021 agccactgca ggatgcccac tgcaacaaag gtgatcacac cggaaacgcg atggagaatg
    43081 gacgctatcg ccgtgatggg gaaccggatg gtctgtaggt ccagattaac aggtctttgt
    43141 tttttcacat ttcttatcat gaataacgcc cacatgctgt tcttattatt ccctggggac
    43201 tacgggcaca gaggttaact ttctgttacc tggagacgtc gggatttcct tcctccggtc
    43261 tgcttgcggg tcagacagcg tcctttccat aactgcgcgt catgcaaaac actgcttcca
    43321 gatgcgaaaa cgacacgtta caacgctggg tggctcggga ttgcagggtg ttccggagac
    43381 ctggcggcag tataggctgt tcacaaaatc attacaatta acctacatat agtttgtcgg
    43441 gttttatcct gaacagtgat ccaggtcacg ataacaacat ttatttaatt tttaatcatc
    43501 taatttgaca atcattcaac aaagttgtta caaacattac caggaaaagc atataatgcg
    43561 taaaagttat gaagtcggta tttcacctaa gattaactta tgtaacagtg tggaagtatt
    43621 gaccaattca ttcgggacag ttattagtgg tagacaagtt taataattcg gattgctaag
    43681 tacttgattc gccatttatt cgtcatcaat ggattcttta cctgcaagcg cccagagctc
    43741 tgtacccagg tttttccctc tttcacagag cggcgagcca aataacaaac gggtaaagcc
    43801 aggttgatgt gcgaaggcaa atttaagttc cggcagtctt acgcaataag gcgctaagga
    43861 gaccttaaat ggctgataca aaagcaaaac tcaacctcaa cggggacaca gctgttgaac
    43921 tggatgtgct gaaaggcacg ctgggtcaag atgttattga tatccgtact ctcggttcaa
    43981 aaggtgtgtt cacctttgac ccaggcttca cttcaaccgc atcctgcgaa tctaaaatta
    44041 cttttattga tggtgatgaa ggtattttgc tgcaccgcgg tttcccgatc gatcagctgg
    44101 cgaccgattc taactaccta gaagtttgtt acatcctgct gaatggtgaa aaaccgactc
    44161 aggaacagta tgacgaattt aaaactacgg tgacccgtca taccatgatc cacgagcaga
    44221 ttacccgtct gttccacgct ttccgtcgcg actcacatcc aatggcagtc atgtgtggta
    44281 ttaccggcgc gctggcggcg ttctatcacg actcgctgga tgttaacaat cctcgtcatc
    44341 gtgaaattgc cgcgttccgc ctgctgtcga aaatgccgac catggccgcg atgtgttaca
    44401 agtattccat tggtcagcca tttgtttatc cgcgcaacga tctctcctat gccggtaact
    44461 tcctgaatat gatgttctcc acgccgtgcg aaccgtatga agttaatccg attctggaac
    44521 gtgctatgga ccgtattctg atcctgcacg ctgaccatga acagaacgcc tctacctcca
    44581 ccgtgcgtac cgctggctct tcgggtgcga acccgtttgc ctgtatcgca gcaggtattg
    44641 cttcactgtg gggacctgcg cacggtggtg ctaacgaagc ggcgctgaaa atgctggaag
    44701 aaattagctc cgttaaacac attccggaat ttgttcgtcg tgcgaaagat aaaaatgatt
    44761 ctttccgcct gatgggcttc ggtcaccgcg tgtacaaaaa ttacgacccg cgcgccaccg
    44821 taatgcgtga aacctgccat gaagttctga aagagctggg caccaaagat gacctgctgg
    44881 aagtggctat ggagctggaa aacatcgcgc tgaacgaccc gtactttatc gagaagaaac
    44941 tgtacccgaa cgtcgatttc tactctggta tcatcctgaa agcgatgggt attccgtctt
    45001 ccatgttcac cgtcattttc gcaatggcac gtaccgttgg ctggatcgcc cactggagcg
    45061 aaatgcacag ttacggtatg aagattgccc gtccgcgtca gctgtataca ggatatgaaa
    45121 aacgcgactt taaaagcgat atcaagcgtt aatggttgat tgctaagttg taaatatttt
    45181 aacccgccgt tcaaatggcg ggttaatttt tatatgccta aacacaaaaa attgtaaaaa
    45241 taaaatccac tatcggacat tcatatattc taaaaaaaaa tacatcggta taaattataa
    45301 acaaatagat gatttttaat catctatctt cctctcagtt acatgaataa accaccaaat
    45361 atagcgcacc cctataattc ccaatacttt attcatcaaa catttaattc ataccatcaa
    45421 ttaattactt ttacatttaa taaaattaat aatattttta agaaaaacta aaaacattcg
    45481 acaatcaaat aagatattct tgcaccgata tacttttttc ttttctgtta aaggactcag
    45541 ttatgtttaa aggacaaaaa acattggccg cactggccgt atctctgctg ttcacagcac
    45601 ctgtttatgc ggctgatgaa ggatccggtg aaattcactt taaaggtgaa gttattgaag
    45661 caccgtgtga aatacatcag gatgatattg ataaagaggt tgaactcggt caggtgacca
    45721 ccagccacat taatcagtca catcacagcg atgccgttgc tgtcgacctg ctcttagtca
    45781 actgtgatct ggaaaactcc agcaacggtt ccggtggcaa gatttctaaa gttgcagtca
    45841 cctttgatag ctcagcgaaa accaccggcg cagatccgat tctcaacaac accagcacgg
    45901 gtgaagccac cggcgtcggc gtacgtttaa tgaataaaga tcaaagcaat atcgttttag
    45961 gcaccgctac tccagatatc gacctggctc cgacctccag cgaacaaacg ctgaatttct
    46021 ttgcctggat ggaacaaatt gatcaggcta cacctgtaac gccaggcgca gttacggcga
    46081 atgcaaccta cgtgctggat tataaataag tattactttg gcggcgctat cgccgccttt
    46141 cataaaatat ttatcaggta tggacaccgt gaatatttat cgactctctt ttgtatcctg
    46201 cctggtcgtg gcgatgcctt gcgcattggc ggtcgaattc aatctgaatg ttctcgataa
    46261 atcaatgcgc gaccgcattg atatttcatt attaaaggaa aaaggagtca ttgctcccgg
    46321 tgagtatttt gttagcgttg cggtaaataa taaccaaatc agtaacgagc aaaagattaa
    46381 ctggcacaaa aatgacgata aaaccattcc gtgcatcaat gatttactgg tcgataaatt
    46441 tggcttaaaa cctgaagtcc gtcagtcgtt accattgata aatcagtgcg tcgattttag
    46501 ctcccgacct gaaatgctct tcaatttcga tcaagccaat cagcaactga atattagtat
    46561 tccgcaagcc tggctggcgt ggcactcaga aaactggacc ccaccctcca catggaaaga
    46621 aggtgtcgcc ggtgtcctga tggattacaa cttgtttgcc agcagctacc gcccacagga
    46681 tggcagcagc agcactaatc ttaacgccta tggaacagcc ggaatcaaca cgggggcatg
    46741 gcgcttacgc agtgattatc agttgaatca tactgatagc gatgataacc atgaacagtc
    46801 aggcgaaata tcacgtacct atctttttcg cccgttaccg caattaggtt caaaattaac
    46861 cctcggcgaa acggatttta gtcccaatat tttcgacggt ttttcttata ccggcgcggc
    46921 actggcaagt gacgatcgaa tgttgccatg ggaactacgc ggctacgccc cacaaattag
    46981 cggtattgca cagaccaatg ccacggtgac gatcagtcaa tcaggccgcg tcatttacca
    47041 gaaaaaagtc ccaccaggcc catttatcat tgacgacctt aatcagtctg ttcagggcac
    47101 actggatgtc aaagtgacgg aagaagatgg tcgggtgaac aatttccagg tttcagcagc
    47161 atcgacgccc ttcctgactc gtcagggaca ggttcgctat aaactggccg cgggtcagcc
    47221 acggccctcc atgtcacatc aaactgaaaa tgaaaccttt tttagcaatg aagtttcctg
    47281 ggggatgctg tcaaacacct cgctgtacag cggcctgctg ctttctggtg atgactacca
    47341 ttctgccgca atgggtattg ggcaaaatat gctgtggctt ggtgcgctgt cgtttgatgt
    47401 cacgtgggcc agtagccatt ttgatactca gcaggacgag cggggcttaa gctaccgttt
    47461 taattacagc aaacaagtgg atgccactaa cagcacgatt tccctcgccg cttatcgttt
    47521 ctccgatcgt cattttcaca gctacgccaa ctatctggat cacaaataca acgacagcga
    47581 tgcgcaggac gaaaaacaga cgatcagctt atctgtgggc caaccgatta ccccactaaa
    47641 cctcaatctt tacgccaacc tgctacatca aacctggtgg aatgcagacg cctccacgac
    47701 cgccaacatc acggcaggtt ttaatgttga tattggtgac tggagagata tctctatttc
    47761 tacgtcattc aatacaaccc attacgaaga taaagatcgc gacaaccaga tttacctgtc
    47821 gatttcgctc cccttcggta acggtggtcg ggttggctat gacatgcaaa acagtagcca
    47881 cagcaccaca caccgcatgt cgtggaacga tacgctggat gaacgtaata gctggggcat
    47941 gtctgccgga ctgcaatccg accgtcctga caatggagcc caggtgagcg gtaactatca
    48001 gcacctgagt tcagcgggtg agtgggatat ttctggtacc tatgccgcca atgattacag
    48061 ttccgtcagc agcagctgga gcggttcttt caccgcaacc caatatggtg cagcgtttca
    48121 tcgccgcagc tccaccaatg aaccacgcct gatggtcagc accgatggcg tggcagatat
    48181 tccggttcag ggcaatctcg actacaccaa ccattttggc attgcggtgg tgccgttgat
    48241 ttccagttac cagccttcca ccgtggcggt gaacatgaat gacttacccg acggcgtaac
    48301 agttgcagaa aacgttatca aagaaacgtg gattgaaggc gcgataggtt acaaatcact
    48361 ggcttcccgt tccggtaaag acgttaacgt catcattcgc aacgccagcg gtcagtttcc
    48421 tcccctcgga gcggatatcc gccaggatga cagcggcatt agcgtgggga tggttggcga
    48481 ggaaggacat gcctggttaa gcggagtcgc tgaaaatcaa aagtttaccg tggtctgggg
    48541 tgatagccag cattgctcgc tccatcttcc tgaacatatg gaagacaccg caaatcgcct
    48601 gattttacct tgtcattaat taaggaaatg aaaatgacat ttatgaaagg actacccctt
    48661 ttgttgttgg ttgccagtct gtgcagccac gctgcactac aacccgatcg cactcgtatt
    48721 gtgtttaacg ctaatgataa agctaccagt ctgcgggtgg ataatcgtag cgataaactc
    48781 ccctacctcg cttattcctg gcacgaaaat gaaaaagggg agaaaagcga tgatcttctg
    48841 gtcgccctgc cgccgataca acgtctggaa ccgaaagcaa caacacaggt acggatagta
    48901 aagcaagcct cgaccacaaa attaccgggc gatcgcgaaa cgctattttt ctacaatatg
    48961 cgtgaaattc ctcccgcccc ggaaaaaaac agcgaccatg ccgttctgca agtcgccatt
    49021 cagagtcgta tcaaggtgtt ctggcgaccg gctgcattac gcaagaaagc aggagaaaag
    49081 gtcgaactgc agttacaggt cagccagcag ggcaaccaac tgacactgaa aaatcctacc
    49141 gcgtattatc tgaccattgc ctacctggga cgaaatgaaa aaggcgttct ccccggcttc
    49201 aaaaccgtga tggttgcgcc ttttagtacg gtcaacacaa acaccggaaa ttatagcggc
    49261 agccagtttt atctcggtta tatggatgat tacggcgcgt tacgcatgac gacgctcaac
    49321 tgcagcggac aatgccattt acaagccgtg gaggcgaaga aatgagtgct ggcaagggag
    49381 tgttgctcgt catctgtctg ttatttctgt cgttgaagtc tgcgcttgcc ctgaactgct
    49441 actttggttc atccggtggc tcggtagaaa aatcagaagc gattcaacct tttgctgtac
    49501 caggcaatgc caaactcggc gataagatct gggaatctga cgatattaaa attcccgtct
    49561 attgtgacaa caataccaac ggcaactttg aaagcgaaca tgtctacgca tgggtaaacc
    49621 cctacccggg ggtgcaggat cgttattatc aactcggagt aacttacaat ggcgttgatt
    49681 atgacgccaa ccagggaaaa agccgcatcg acactaacca gtgtatcgac agtaagaaca
    49741 ttgacattta tacccctgat gggatggcag aacaaaattt gctcgggtga tcccgctaat
    49801 attcacatgt cgcgaacctt tctcgctcgc atgcggttat acgtcaaaat acgagaaatg
    49861 ccgccgcatg attatcaaag tacgcttagc gactatatcg tcgtgcaatt tgacggtgcc
    49921 ggtagcgtta acgaagaccc tactgcccaa aacctgaaat atcatattac tggtctggaa
    49981 aacattcgcg tgctggattg cagcgtcaat ttttccattt caccggaaac acagatgatt
    50041 gattttggta aatttaattt gctggatatt cgtcgccaca caatgtcgaa aacattcagt
    50101 attaagacga caaaatcaca aaatgaccaa tgcaccgacg gatttaaagt cagttcctcc
    50161 ttttataccg aagaaacgtt ggttgaagaa gataaagcat tgttgattgg taatggtctg
    50221 aagctacgtt tgctggatga aaacgcctcg ccttatacct tcaataaata tgctgaatac
    50281 gccgatttca ccagcgacat gttggtctac gaaaaaacct atacagctga actttcgtcc
    50341 attgcaggta cccccatcga ggctgggccc ttcgatacag tggtgctttt taaaattaac
    50401 tataactgat ttgccatgat gccctttgca gccggaaagg gcatctcttt cagaaaattg
    50461 ctgattttcc ccttttcctt gattgctgtt attttttcct agcccggctt tctttatgct
    50521 gtatcgatac accaaagaga aaataatgag ggagcgaagg atgccagttt tgcagtgggg
    50581 aatgttatgt gtgctatcac ttctcctttc tattggtttc ctcgcggtac atctcccggc
    50641 ggcgctatta cttgggccga tgatcgccgg gatcatcttc agtatgcgcg gaattactct
    50701 gcaacttccc cgctccgctt ttctcgccgc gcaggccatt cttggctgca tgattgcgca
    50761 aaacctcact ggttcgattc tcaccacgct ggccgccaac tggccgatcg tgcttgctat
    50821 tttactggtg acgctgcttt ccagcgccat cgtgggttgg ttattggtgc gctatagctc
    50881 actgcctgga aataccggtg cctggggttc ttctccaggc ggcgcagcgg caatggtcgc
    50941 tatggcgcaa gattacggcg cagatattcg cctggtggcg tttatgcaat atctgcgagt
    51001 gctgttcgtc gcgggggccg ctgttctggt cacccgcatg atgctgggtg ataacgctga
    51061 agcggttaac cagcaaatcg tctggttccc gccagtgagc attaatctcc tgcttacgat
    51121 tttgctcgca gtcgttgccg gtacggcggg atgcatgctg cgtcttcctt ccggcacgat
    51181 gctcatcccg atgctggcgg gcgcagtgct ccagtctggt cagctcatca ccatcgaact
    51241 accggaatgg ctgctggcga tggcgtatat ggcaattggc tggcggattg gtcttggttt
    51301 cgataaacaa atcttactgc gggcactacg cccgctaccg caaatcctgc tgtcgatttt
    51361 tgctctgctg gctatttgtg caggtatggc gtggggactg acccggttta tgcatattga
    51421 ttttatgact gcctacctcg ccaccagtcc cggcgggctt gataccgtgg cggtcatcgc
    51481 cgcagggagc aatgccgata tggcgcttat tatggcgatg caaaccctgc gcctgttcag
    51541 tattttgctg acggggccag ccattgcgcg gtttatttca acctatgcgc cgaagcggtc
    51601 ggcctagtgc tggcagcctg gacaccagta aaacgggcgc gaggacaacg tggttttctc
    51661 aatgatgccg ccacagcgtt cgcacagttc gccatcacga tgaaaaacct taaagcgaaa
    51721 cagcgcccca tgatgcttat tctcatccac ctgcccccgc gtagcgtaag aaaaccgagg
    51781 aatctccagt aacgcgtgtg cgagtgcatc cagttgcgcc gcattgagat ctttcgcttt
    51841 atgatttcca gtcaacccaa cctgccagag gatctccacc cgcaaataat tgccaagccc
    51901 agccagaaac gcctgatcga gcagtaatcc tgcaaactga cggttacgaa agcgcggcga
    51961 caataaccgt tcctttacca cctccggcgt cagattcgga tccagcacat cgggaccaac
    52021 gcgttgtaaa aacggatgcg tggtcagttg ttccggcctc aacatctcaa tatccgaggc
    52081 gctataaagc agaatggttt tgtcagccgt ttgcagtttt acccgcaata ctcgcgtggt
    52141 ctgcggctct tcgccggtat caaccacgcg ccagacgccg taaagctgat tatggctgta
    52201 gagcgttaaa ccgttgggaa aatgagttaa caacgcctta ccacgcgttt ccacatgggt
    52261 aacgtgttga ccgataagtt gtgattgata agtttttaac tgcgggaagg caaaccagac
    52321 atcagttagt ggtttgcctt tgatcgccgc ctccaggtta tccgctgcac ggcggatctc
    52381 cgggccttca ggcatggtga tatccttttt tcattcttta ttaagcgggg ttatgctgcg
    52441 acaacaatcc cctttttggc aaatgtagct cgtaatcggc gggcgaacgc cagagcgtgc
    52501 tcgccgtcgc catgcaggca gaccgtttgc gccgcgaccg ttgcccattc gccggtgata
    52561 cttttgactc tgccgtgttg caccatctcc agcgtttgcg ccaacgcctg ttcttcgttt
    52621 tcaatcaacg cgcctggctg acttcgcggc accagcgagc cgtcagcctg ataaccgcga
    52681 tcggcaaaca cttcctcgcg cgttgtcagg ccatattgct tgcctgcacg aatcagctcg
    52741 cttcctgcca gcccaacgag aaccaatgcc ggatcgcaag cgtataccgc tctggcgatg
    52801 gcgtctgcca gttgtgcttc tttcgccgcc tggttgtaca acatgccgtg cggtttgaca
    52861 tgacgcatta cgccgccttg cgcacgggca atcgttgcca gcgcgccaat ttgatacagc
    52921 gtctgggcgt aaacggtttc cggcggcagc tgcatggcgc tgcgaccaaa attttccctg
    52981 tcgggaaaac tcgggtgagc gccaatcgcg acaccatttt ttattgcttc acgcacgcaa
    53041 gcctgcatgg tttgcgcatc gcctgcatga aatccacaag caatattggc agaggaaacc
    53101 agcgttaata gctctgcgtc gctggcgcag ccttcgccca gatcggcgtt caggtcaatt
    53161 ttcattgtgc agccgccacg ctaattgttc aaaataacgt tgctgatctt gccgcgcttt
    53221 cagtgcctct tccagtgaac actggacaaa atgaatcggc tgaccgagcg gaatttgcgc
    53281 cagatggtac atatcagcct caatgataca ggcaatacgt ggataaccac cggtggtctg
    53341 tgcgtcgttc atcaacacaa tcggctgccc gttatgcggc acctgcacca cgctcggtaa
    53401 caaaccgtga gataacagtt cgcgatcggt gatgcgtttt aaaatttgcc cctgtaaacg
    53461 atagcccatt cggttacttt gcgagctaag ctgccagggc gaacgccaga atgcatcctg
    53521 cgaggcgcga tcgaactcat gatattccgg ccccggcaag gcgcgaatgc ggttgcccca
    53581 cagcagctgt ttaacgccct gcgcttccat aaaatcacgc ttcgatttgc caatcgggag
    53641 tcagtcacca tccttcagta aacggccttc cagcccgcca atccccactt tgagatcggt
    53701 gctgcatgac cccattaccg gcggaacatc aataccaccc gcgaccgcca gataactgcg
    53761 catcccgtgc tgcgggcgtt taagcgttaa acgctggcct gctttcatcg gcaatcgcca
    53821 gccggtccag acggcgttat catccaaccg cgcttcgcaa cctgcacccg tcagggcaaa
    53881 ccacccatca gtttcgaact caactgttaa ctgaccgagc gtgatctcca gcgcgggggc
    53941 attggcgtca ttacccacca gcaggttagc aatgcgcagc gcaggcatat ccagtgcgcc
    54001 acagtggctg atacccgact ggcaaaaacc gtgacgaccg ccatcctgca cagtggtgta
    54061 catgcccgca cgaataatct tcagcatatt ccctccttct gcggtacaaa tcgcacgctg
    54121 tctcccggac gtaataagat gggttcgtca cgcgccggat caaacaggct gagtgaggta
    54181 tgaccaatca actgccagcc acccggcgtt gccagcggat aaacaccagt ctgcggcccg
    54241 ccgatcccga cagaacctgc cggaacgagc aagcgcggtt cagcgcgccg tggcgtgtgt
    54301 agttgttccg gcaaactccc gagatacggg aagcccggtt gaaaacctaa aaaccagacc
    54361 acgtattcca cggaggagtg caattcaaca acctgttttt cgctcaaccc gcaatacgcc
    54421 gcgaccaccg ccaaatccgg tccgcctgca ccaccgtaaa ccaccggaat ttcaataaag
    54481 cgagactcag gttccagggc ctcgctctcc tcccaccagc gttgcaaacg ctctatggca
    54541 tccagcgcca gcgactcagg attacgcaaa atcaccgtga tattgttcat gccgggaatg
    54601 gcttcaacca cattcggcat atccaccaga cgctgcgcca gtcgccagat ccgtttctgg
    54661 ctagccagcg tcaccggcgg ttccagttcc agcactaccg ccgtttcacc tatcagataa
    54721 caacgcgctc gttgcacttt cgatccctct gattattcgt tatgcaggat taggaatatc
    54781 aataaaggtc acatcaagat cggtattttc attcagccac tcgctcaatg cgcgaatacc
    54841 accgcgttca gtggcatggt gacctgcagc ataaaaatgc aatccctgct cgcgggctga
    54901 atgaatggtc tgttcagaaa cttcgccagt aataaaagca tccacgccaa aacgcgcggc
    54961 gctatcgata aaactttgcc cgccgcccgt gcaccaggcg acgcgctgga ccacctcagg
    55021 tccggtatcg ccacaccata acggcttacg tcccagacgc gcttcaatcc aggaggctag
    55081 ttccattccc ggcacaggca tggtcagctc tccccacggc accaacggct caatttcgcc
    55141 catgaccgtg atccccagta acgccgccag ctgtgcgtta ttgcccagct caggatgtgc
    55201 gtcaagcggc aaatgccagc catacaggtt gatatcattc gccagcaacg tttttaaacg
    55261 gttacgcttc attccgcgaa tgaccggaga ctctcctttc cagaagtagc catgatgcac
    55321 aatgaccgca tctgcgccca gacgcactgc ctcatcgagc aaagcctggc tggcggtgac
    55381 accggtaaca attttttgca ccgtctcttt gccttccacc tgcaaaccat tcggcgcgta
    55441 atcactaatc gccgcgctgt tcagtttttc gttaatcagt tgttccagtt cggtgttttt
    55501 catcttcgcg ccttccgtat tctgatagct acaccgttaa catagcgtta catcgcctgt
    55561 tcgtcgatag ccaatctttt gacatattaa gaaacaaacc cgacgccaaa agacgcaaat
    55621 tgtttatatc atcacgcttc agctaatgta tatttatgca atcagacagc atattgcata
    55681 aataatcaga tctgtatcaa agttttaact ttaaggaaac acaagcacgc cgcgcaacta
    55741 ttacgtgacg ttgccgtcaa ttgtggaatt attatgaata aacacgcatc acaaccgcgc
    55801 gctatttact atgtcgttgc gctgcaaatc tgggaatatt ttagctttta cggcatgcgt
    55861 gccctgctga ttctctatct caccaatcaa ctaaaataca acgatactca cgcctacgag
    55921 ttatttagcg cctactgttc gctggtgtat gtcacgccaa tcctcggtgg ctttttggcg
    55981 gataaagttc tcggcaatcg catggcggtg atgctggggg cgttgttgat ggcgatcggt
    56041 catgtggtgc tgggtgccag tgagatccat ccgtcattcc tctatctgtc cctggcgatt
    56101 atcgtctgcg gctatggcct gtttaaatct aacgtaagct gtctgctcgg cgagctgtat
    56161 gagccaaccg atccgcgtcg tgatggcggt ttctcgctga tgtatgcggc gggtaacgtg
    56221 ggatctatta tcgcacctat cgcctgtggt tacgcccagg aagagtacag ctgggcgatg
    56281 ggctttggcc tggcggcggt tggcatgatc gcgggtctgg tcattttctt atgtggcaat
    56341 cgtcatttca ctcatacccg cggcgttaac aaaaaagtac tgcgtgcgac aaactttctc
    56401 ctgccgaact ggggatggct gctggttctg ctggtggcaa cgcctgcact gattaccgtg
    56461 ctgttctgga aagagtggtc ggtatacgcc ttaattgtcg cgactattat tggtctgggt
    56521 gtactggcaa aaatttatcg caaagcagaa aaccagaaac agcggaagga gctggggctg
    56581 attgtgacgc tcaccttctt cagtatgttg ttctgggcct tcgcacaaca gggcggtagc
    56641 tcgattagcc tttatatcga ccgcttcgtt aaccgcgata tgtttggtta taccgttccg
    56701 accgcgatgt tccagtcgat taatgccttc gcagttatgc tgtgcggtgt gttcctggcg
    56761 tgggtggtta aagaaagcgt cgcaggtaat cgtaccgtgc gcatctgggg aaaatttgct
    56821 cttggccttg gcctgatgag cgccggattc tgcattctga ccttaagcgc ccgctggtcc
    56881 gcaatgtatg gtcactcttc tctgccactg atggtgttag gcctcgcggt gatgggcttt
    56941 gcggaactgt ttatcgaccc ggttgccatg tcgcaaatta cgcgcattga aattccgggc
    57001 gtgaccggcg tattaaccgg catctacatg ctgctttctg gcgcgatcgc gaactatctg
    57061 gcgggcgtga ttgccgatca gacatcgcag gcttcgtttg atgcttccgg ggcgatcaac
    57121 tactccatca atgcatatat tgaagtattt gatcaaatta cctggggcgc actggcgtgt
    57181 gtaggactgg tactgatgat ttggctgtat caggcgctga aattcagaaa ccgcgcgctg
    57241 gcgctggagt cataataggc cagcaccgat gcctgatgcg acgcttgacg cgtcttatca
    57301 ggcctacgta tttcctgcaa tttattgaat ttgcacaaat ttgtaggctg gattaggcgt
    57361 tcacgccgca tccggcatct gggctcgatt gcctgacgcg tctgttattt ccccttccgc
    57421 gccgcctcat acgctgccaa cgtttgtact ctcgcctctt tgtgctcgac tatcggttgc
    57481 ggataatcca gcttcacacc tgctttctgc gcccacttcc acggctcatg caccgctttc
    57541 cctggtacat cgcgcagctc cggtaaccat cgacgaataa actcgccctc acggtcaaat
    57601 ttctcgccct gagttatcgg attgaaaata cgaaaatacg gcgcagcatc agtacccgta
    57661 gaagccgccc actgccagcc accgttatta gctgccaaat caccatcaat cagctgcgac
    57721 atgaaatatc gctcgccttc gcgccagtcg atcaacaaat ctttcaccag aaaactggct
    57781 gtaatcatcc gtagccggtt atgcatccag ccagtgctgt taagctgacg catagcggca
    57841 tcaataatcg ggtatcccgt tttgcctttc tgccaggcct gtaaatgtgc gggattgctt
    57901 tgccactgca cacgatccgt ccaggcaata aatggacgat gtttacacaa cgaggggtaa
    57961 tacgtcatca gatgacggta gaactcgcgc cagatcagct cactaagcca gacactaccg
    58021 gccccaccgt ccagcgcctg cggctgttcc gccaacaagc gatgcaaaca ctggcgaggc
    58081 gataacccgc ccgttgccaa gctggcggac aaacggctgg tgccttccac tgccggaaaa
    58141 tctcgttgtt gctcatactc tccagcaccg ttctcgcaaa actggcgtaa ttgcgcaatc
    58201 gccgcttttt cttccaccgg gaaatgcgca gtatcgaaag actgacgagg ataattcagc
    58261 gtaatggatg gcgcgggctt tatcgatccg ctactacgaa cttttggtgc agcgacgcac
    58321 tccggcatcc cttcccgcag ccgtttcagc caggcattct taaaaggcgt aaagactttg
    58381 tacatctcgt gattgccggt catcaccgcc cccggcggca ggatcacgct gtcatcaaat
    58441 ccttcacaca ccacgttacg cagcgctctt tcaacgtcca catcccgcgc tcgctcattc
    58501 acctcatact gatagttata aaaaagatga gtaacgctgt tttccgcgca cacctgttta
    58561 actatttcga cactggcgac aaagtcatcc acttcacgga acaataaagg aatacctttt
    58621 tccgccagcg cgatttgtag cccattcagt tgagcattga taagttcagc ctgacgcggc
    58681 gacatgttat gcgtctccca ctggcgtggt gtagcgatat acagcgccag cacgcgtgca
    58741 gacgaattgc ggcaggcggc agccagtgcg agattatcgt gcagacgtaa atcctggcga
    58801 aaccaggcca gatgggtagt cataaaactc ctgaatggcg caagttaatc acacgcggcc
    58861 aaagttcgaa ataacgctgc cctgaaagcc aggcgtcagg ataaagcgcc atatagtgtt
    58921 tgatacgcat cagcggcgca agaagtggtt gctcgccacg gcgataactg tcgataagcg
    58981 cagccagctg ctggcgttct gtggaatcaa tatgcgggcg aaaataaccc tgaacgtgca
    59041 tcaaaacgtt ggtgtgatcg cgcgggttgg cgggatgaga aagcaaaaca attacgcgtt
    59101 ggcggtactg gttgtaaaaa tcatccagat tttgccactg atgaatatca gccacaaacg
    59161 ggccaagttt gcggtactcg ggctgagaat gggccagaaa gacgagttta tagcggctgt
    59221 gataatcgag aagcgccccg cgcgttaagc catgttcttt cagtcgatgt aactcatgaa
    59281 gggcgaagat acggattagg gtgctgtcat caaatcgttg tagattcatt actgctcctg
    59341 aacggtatac aggagtaagt gtaggcgttg gtaaatgatg tttgtgaagt taatccgcta
    59401 gcagggcaag attacgaaag cgcgctcccc gcaagcaact aatcaaggta gttaatcatg
    59461 gctgaacacg atattacgcc agaagtcacc atttccaaaa cacagcgccg ctataaagtc
    59521 ggctatgtca gagcccgcca ccaggaccgc aaaaccggta tagcccggta ttattcacaa
    59581 catcccagcc tgcaccttaa gggcgactgg ctgaaagagg ccggatttga taccggacgg
    59641 ggtgtcacgg tgaagatttc ggaggggggc ctgaccatca tcgctgacag tgacgaggtg
    59701 caggaactgc gggatgggat gtttagtgtg ctgaacgaaa gttaaatatt aaaaaagctg
    59761 ggaagattta aaaccttcct agctttttat tctgtcttat tataatggat actcatcatc
    59821 ttccgatgaa tttataaatt gctccaagtt tacatgaaga gtacttaacc tagtttttaa
    59881 ctcattaagt gaagcgataa tttcatattt tccaacataa tcatatgatg gatcgtattc
    59941 attatctatg tcagaagcta aaaatgatga tattcgtagt ttaaatttag cttcatcttt
    60001 attacattca ttggcataag gtgaatgccc tatcatagca tattcatcta atctaaaagg
    60061 ataagatggt tttaattcat cgatcaatga aatttcaatc catgttctat atccaggcat
    60121 taatggacta atttctttga gcaaaacctt tccacttaat aatgtattga gattgttcat
    60181 tattatgtct cccacgggaa aaatatatgt ctaacacttc caccatgttg atgaacttgt
    60241 aaatgtggta aatcaccatc aggatttcct agtccatgac ctgacactct atatgaaggt
    60301 tttgttacag aaggatcttt tatatcatcc caatgatatg aacctgcttt tgttttattt
    60361 ccagtaagat attccattaa gttcttagtg ctggggcctg actcacctgt catattttta
    60421 taattatgac ctaaatatct atccataaac aattgttcag cgtcacttct agacctaaca
    60481 gtaactttgg tttcactact atctaataaa cgattcgctt tttggttact aacagcacat
    60541 ggagttaatc caagtggatc agcatatttt agaggattag gtgcataact gtagagattt
    60601 aaaccgcctc tcaaccctat cgggtcagtt accatgaacc gcccgacatc cggatcatag
    60661 tacctgtgca gattgtagtg cagccccgtt tccctgtcga ggtactgtcc ctggtagcgc
    60721 aggttctgct ccaccccgcc gtggtccttc tcctgtatcg tgttacccca gacctgatac
    60781 cccgtctccc acacgatttt cccgtcgctg tccgtcatct cttccggcgc gccgttcaca
    60841 tccgtgtgaa aatacagtat ccggttcggg ccctctttgc ccgccttatc cacccgcgcc
    60901 agcggttcat aactcccctg gtccctgtag atgtacaggc tgcctgttcc ctccggcctg
    60961 ctctcctgcg ccagccgcat tccgtcccat aaaaattttg tccggttgta cggcttaccg
    61021 tcagggctga tgtgctgttt ttccgtccgc ctccccagtg catcgtacac ataccggtat
    61081 gtcccccgcg ctgtcgtcac ttccaccatc cggtgttccg catcataacg gtaactctgc
    61141 gttccgctcc ggccttcctt ttccaccgca cggccaaatt cgtcataacg gtagtgcaca
    61201 ccacggtaag acatcagccg gttgaacggc accacgctgt tttctgccgt ggccctttcc
    61261 cccgcgcgcc tccccagcag gtttcccgcc gcgtcgtact gccagctcgc cagatacccc
    61321 tcatcctccg aacgacacgc cgtgatgcgc cccgtggggt cgtagaagta atccgtttcc
    61381 ccccgccggc tgtgccgcgt cttcagcagt tcatcctcgc cactgtagct gtactgccgg
    61441 tccgtgaacg tctccggtac caccccgcgc atcccgctgt atgtctccct gcgcttcagc
    61501 cgcccgccgg ggtcgtaaag ctgccgcgtg ctcagtcgcc cctggcttct gagcacttca
    61561 cggtgcaggt ggtcgcgcgt gaactcgctg atgacctccc ggtccagatt tatctgctgg
    61621 agatggccgc tgccgtagta cagatggcgc tgcgttctgc cgtccggaaa ggtggtggca
    61681 gtccggttgc ccagtttatc gtactggtgg gtcaggatgc cctgcgccgt ctcctcgctc
    61741 agcatccgcc ccagtctgtc gtagctgaag cggataactt ccggcgcgtc agtttcgtgc
    61801 cgctggcgac ggatttccag tagctggtct gccgcatcat ggacgtattc cgtacggcca
    61861 tcggctgtct cttttgccgt gagccggccc gccgcatcac gcgccagccg gtggcggatt
    61921 tcaccgcccc ggtccccggt gaacaccact tccgtgaccg cgttcagcgc gttgtagcgg
    61981 tacgactggc ggcagccgtc cgggtttatc tgctccgcca gacggtccag cacatcatgg
    62041 cggaaccggt agcgttctcc gttctcgttc gtgagcgtga ccagacgccc gtaagcgtcg
    62101 tacccgaatg ccgtcctgcg ccccatcgcg tccgtccggc tgcgcacccg gccctgcccg
    62161 tcccgctgcc agcgcgttat cctccccatc gggctggtgt actttaccag cctgcccgcc
    62221 gcgtctgact ggtaattcac cgtgcggccg tccgccagta tcacctccgt cagatgaccg
    62281 ctgtcgtcat acccgtagcg cgtgctgtgg ctctgcgcat ccgtcattct gataagcctc
    62341 gtgcgttcgt cgtagaacca cgccgtctgc gagccggaac agtccgtgtg ccgcatgagc
    62401 tgcccgtctt cattccactg gaggtattta ttccccccgc gcgcatccgt tatctgcacc
    62461 acctagccgt ggtggtcata ctgataccgg gtgttctgct gcagcgggtc ggtcacggca
    62521 agaaggttgc cgcgttcatc gtacacacag cgccaggtgt ttcccgccgc gtccacctcc
    62581 gtttccggct ggtgccacac cgggtgccac tgcgcctgct ccacccgccc cagcgggtcg
    62641 tgcgtctccg tgatatgccc ctgcaggtca tacacatagc gccatttgcc gccctgaggg
    62701 tcggtcatcc ccagcaacag tcgttcttcg tcgctccagc ggaatgtcgt cacctgtccg
    62761 gcttcgtcct ggtacgccgt cacctgattc tgcgcatccc actggtgata acgtgtgcgc
    62821 cccagtccgt ccgtcacgca cagcctgcgc tgcgccaggt cccacgccag ccggtactgt
    62881 tcaccgctgt ccgtgtggtg ccccgtcacc cgccatgccg cgtcatccgg accttcatac
    62941 cagccccact ggtaataata cccttcgccg cccggctggc ggtgcagcgt catgcagtgg
    63001 ttttcgtcgt aagcaaactc acgcagcagg tgccccgtgc tgtcgtacac gccgttcagg
    63061 tggccggcct cgtcatagct gtaacgacgc agcagtaaag gtgcagtctc agggaaatgc
    63121 tgataaatct gtgtcacccg ccggggatgc gccgccagtt cgtaatacag acggatgcac
    63181 ggacgctgct gttcgccgct gatttccaca atgcggcccc ggtcgtcgta gtacagattc
    63241 agacagttgc tgttacggtc gcccagcatt ttcagccgct ggcgctgcgg gtggtgcggg
    63301 tcagcctcaa acagaaaaca ctgcccgtca tcgctgctga taagccagtg cccctgctca
    63361 ccacgcctta ccgccagccc ttcaccgggg ctgtaaaacc cgccccccgg cagtacggct
    63421 tccatatcca agcgtcgccc catgcccgac acaaaggtca tgcagttttc gtctgcgcta
    63481 cccggtgtgc acacgaggct gactttatac attacactcc agccctgacc aaacattcct
    63541 tccgtacgtt cgtcacgact gctgtacacg cgctgccagt ccagcggaaa gtggcccggc
    63601 agactgaaat cgaagtcatc gtcatcggcc aggtatttgg ccccggtggc ggcattgacc
    63661 ggatgcggaa gggcactggt ggccttctgc gtcagcatgc tcatcccgac actggcagca
    63721 aaacaggcga ttttagaaag cattttgccc gggcgaagaa acatcagcgc cacggttgcc
    63781 accagggtta tctgcgacct gccgcttctg atatccctga ccaccagtaa cggaccgcca
    63841 atgcgcacat ccggtgacac aaacgccccg ttccccggct cattcaccac cctggcctcg
    63901 caggtacagc gtgccccgct gcgtgtcgcc ggctggctgt tgatacgcac atcccgcgcg
    63961 ccttccgcga tatattccgc tgcgggcggc tcgtcatcgc ctttgaacag attactgatg
    64021 ctgccaataa ccgcattcac tccctgaaag gcggtttcca gcataccgcc cggattcagc
    64081 aggttaaggg ccgttcccag ggggtcatcc agaaaggcgg cttttttttg tgcgagaaac
    64141 tgcgtcagtg aggaaggatg tttatggcag tcgattttat cgtcaggggt cggggtggat
    64201 cccggggccg cactggccac agtgggctgc catagttccg acacaaaatg tgtcacgcca
    64261 tccaccattt cagaacgccc ttcttccgta ctgagctggt cgtgcagagc ccgggacatc
    64321 agcacaggcc ctgtcaccgg gttaaccgcc agggctatgg cccccgccat ttccagatat
    64381 tccccggcca catcccggaa cgttctggca tcctcctgac gctgcgcttc ttcctgttct
    64441 gccttcgcct gcgcttcctg cgcctctatg tcctgccgcg atgccgcagt cgccgctgcc
    64501 cgcgcggcag gtttgctgtt gatccatgtg tcaccagagc cggtgaggat gtaaccttca
    64561 atctgaggcg ggaaaagcat atttgcgagg gctgaacagg cttcgctgac cttttctgtc
    64621 acgccggtgt acaccatcgc cacacccgcc acaaaaccgg tcagcacgac ggccgtgcac
    64681 cccatcgtga tccccactga cgtcgccagt aacgaggttg tgccgacaat gagggcatca
    64741 ataacgacat ttgcggccac ctccagcacc gcccccagca catctgccat aaacgacgta
    64801 tgcagcaggg catcaccctg tcttgccgcc cagaatgcct cttccatgtc ccgttccctg
    64861 tatgattaat tcgtcctgac ttcctgccgc atcctgaagc gcagattctt tttaatttca
    64921 gcccagcgcg tcatatctgc cggggtcagc ggtgtggttc tggcgtaggt gaaggccatc
    64981 atgacccgct cacccggcaa tacaaatgcc acctgacact ggtacacctg ctcgttaccc
    65041 cggagaaact ggctctgtat ttccaaaccg cctcgttgag ataatattcc gtcataatgt
    65101 aaatagtccg ttttaataaa ggaattattt attgacaggt tctgtttttt taatcatgaa
    65161 tgtaataccc atcaccgctt tacagcacac tttatctttc cagccataat cactaataat
    65221 attaagcaat tagcaatatt tacaacgacc agagcggata catagttatg tatttaacaa
    65281 tcccaatata ttcactatgc aaataaaacc actagtaaca gacacgctac cgcctccata
    65341 taaaaatgga caccttataa aattgtatct tgcatagaaa aagctgaaat atcggatgcg
    65401 ttttaattat caactataaa tctataagct ttttattaac aacaa
//
LOCUS       DAEEGA010000012        60413 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEEGA010000012 DAEEGA010000000
VERSION     DAEEGA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60413
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.012"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..316
                     /locus_tag="KLH46_000780"
     CDS             <1..316
                     /locus_tag="KLH46_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0377579.1"
                     /translation="GTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSN
                     SVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVT
                     YP"
     gene            429..1223
                     /gene="panB"
                     /locus_tag="KLH46_000781"
     CDS             429..1223
                     /gene="panB"
                     /locus_tag="KLH46_000781"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBE0377580.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1235..2086
                     /gene="panC"
                     /locus_tag="KLH46_000782"
     CDS             1235..2086
                     /gene="panC"
                     /locus_tag="KLH46_000782"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBE0377581.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="KLH46_000783"
     CDS             complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="KLH46_000783"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBE0377582.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3336..3716
                     /gene="panD"
                     /locus_tag="KLH46_000784"
     CDS             3336..3716
                     /gene="panD"
                     /locus_tag="KLH46_000784"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBE0377583.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3720..4949)
                     /locus_tag="KLH46_000785"
     CDS             complement(3720..4949)
                     /locus_tag="KLH46_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBE0377584.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5013..5453)
                     /locus_tag="KLH46_000786"
     CDS             complement(5013..5453)
                     /locus_tag="KLH46_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE0377585.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5558..6328)
                     /locus_tag="KLH46_000787"
     CDS             complement(5558..6328)
                     /locus_tag="KLH46_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0377586.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6325..7251)
                     /locus_tag="KLH46_000788"
     CDS             complement(6325..7251)
                     /locus_tag="KLH46_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0377587.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STAIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7360..8022
                     /gene="can"
                     /locus_tag="KLH46_000789"
     CDS             7360..8022
                     /gene="can"
                     /locus_tag="KLH46_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBE0377588.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYSIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="KLH46_000790"
     CDS             complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="KLH46_000790"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBE0377589.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8805..11195
                     /gene="gcd"
                     /locus_tag="KLH46_000791"
     CDS             8805..11195
                     /gene="gcd"
                     /locus_tag="KLH46_000791"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBE0377590.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="KLH46_000792"
     CDS             complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="KLH46_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBE0377591.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12958..13305
                     /locus_tag="KLH46_000793"
     CDS             12958..13305
                     /locus_tag="KLH46_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBE0377592.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13411..14277
                     /gene="speE"
                     /locus_tag="KLH46_000794"
     CDS             13411..14277
                     /gene="speE"
                     /locus_tag="KLH46_000794"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBE0377593.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14293..15087
                     /gene="speD"
                     /locus_tag="KLH46_000795"
     CDS             14293..15087
                     /gene="speD"
                     /locus_tag="KLH46_000795"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBE0377594.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15125..15487)
                     /locus_tag="KLH46_000796"
     CDS             complement(15125..15487)
                     /locus_tag="KLH46_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBE0377595.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="KLH46_000797"
     CDS             complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="KLH46_000797"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBE0377596.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18614..20467
                     /locus_tag="KLH46_000798"
                     /pseudo
     CDS             18614..20467
                     /locus_tag="KLH46_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="KLH46_000799"
     CDS             complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="KLH46_000799"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBE0377597.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="KLH46_000800"
     CDS             complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="KLH46_000800"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBE0377598.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="KLH46_000801"
     CDS             complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="KLH46_000801"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBE0377599.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="KLH46_000802"
     CDS             complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="KLH46_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBE0377600.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28311..29684
                     /gene="aroP"
                     /locus_tag="KLH46_000803"
     CDS             28311..29684
                     /gene="aroP"
                     /locus_tag="KLH46_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBE0377601.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWTMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="KLH46_000804"
     CDS             complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="KLH46_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBE0377602.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="KLH46_000805"
     CDS             complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="KLH46_000805"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBE0377603.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31217..32110
                     /gene="nadC"
                     /locus_tag="KLH46_000806"
     CDS             31217..32110
                     /gene="nadC"
                     /locus_tag="KLH46_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBE0377604.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32313..32753
                     /gene="ppdD"
                     /locus_tag="KLH46_000807"
     CDS             32313..32753
                     /gene="ppdD"
                     /locus_tag="KLH46_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBE0377605.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32763..33910
                     /gene="tadA"
                     /locus_tag="KLH46_000808"
                     /pseudo
     CDS             32763..33910
                     /gene="tadA"
                     /locus_tag="KLH46_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33900..35102
                     /gene="hofC"
                     /locus_tag="KLH46_000809"
     CDS             33900..35102
                     /gene="hofC"
                     /locus_tag="KLH46_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBE0377606.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="KLH46_000810"
     CDS             complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="KLH46_000810"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBE0377607.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36342..36380)
                     /locus_tag="KLH46_000811"
     CDS             complement(36342..36380)
                     /locus_tag="KLH46_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377608.1"
                     /translation="MIIKTLKMSARK"
     gene            36405..37025
                     /gene="coaE"
                     /locus_tag="KLH46_000812"
     CDS             36405..37025
                     /gene="coaE"
                     /locus_tag="KLH46_000812"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBE0377609.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37025..37768
                     /gene="zapD"
                     /locus_tag="KLH46_000813"
     CDS             37025..37768
                     /gene="zapD"
                     /locus_tag="KLH46_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBE0377610.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37778..37975
                     /gene="yacG"
                     /locus_tag="KLH46_000814"
     CDS             37778..37975
                     /gene="yacG"
                     /locus_tag="KLH46_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBE0377611.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="KLH46_000815"
     CDS             complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="KLH46_000815"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBE0377612.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38524..41229)
                     /gene="secA"
                     /locus_tag="KLH46_000816"
     CDS             complement(38524..41229)
                     /gene="secA"
                     /locus_tag="KLH46_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBE0377613.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41291..41803)
                     /gene="secM"
                     /locus_tag="KLH46_000817"
     CDS             complement(41291..41803)
                     /gene="secM"
                     /locus_tag="KLH46_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBE0377614.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="KLH46_000818"
     CDS             complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="KLH46_000818"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBE0377615.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="KLH46_000819"
     CDS             complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="KLH46_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBE0377616.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="KLH46_000820"
     CDS             complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="KLH46_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBE0377617.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="KLH46_000821"
     CDS             complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="KLH46_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBE0377618.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="KLH46_000822"
     CDS             complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="KLH46_000822"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBE0377619.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47268..48743)
                     /gene="murC"
                     /locus_tag="KLH46_000823"
     CDS             complement(47268..48743)
                     /gene="murC"
                     /locus_tag="KLH46_000823"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBE0377620.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48797..49864)
                     /gene="murG"
                     /locus_tag="KLH46_000824"
     CDS             complement(48797..49864)
                     /gene="murG"
                     /locus_tag="KLH46_000824"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBE0377621.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="KLH46_000825"
     CDS             complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="KLH46_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBE0377622.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51105..52421)
                     /gene="murD"
                     /locus_tag="KLH46_000826"
     CDS             complement(51105..52421)
                     /gene="murD"
                     /locus_tag="KLH46_000826"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBE0377623.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="KLH46_000827"
     CDS             complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="KLH46_000827"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBE0377624.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53500..54858)
                     /gene="murF"
                     /locus_tag="KLH46_000828"
     CDS             complement(53500..54858)
                     /gene="murF"
                     /locus_tag="KLH46_000828"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBE0377625.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDLADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54855..56342)
                     /gene="murE"
                     /locus_tag="KLH46_000829"
     CDS             complement(54855..56342)
                     /gene="murE"
                     /locus_tag="KLH46_000829"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBE0377626.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSCVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="KLH46_000830"
     CDS             complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="KLH46_000830"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBE0377627.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKESMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="KLH46_000831"
     CDS             complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="KLH46_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBE0377628.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="KLH46_000832"
     CDS             complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="KLH46_000832"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBE0377629.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="KLH46_000833"
     CDS             complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="KLH46_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBE0377630.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14472 a        16733 c        14846 g        14362 t
ORIGIN      
        1 tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgatg cagcaaaagg
       61 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
      121 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
      181 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
      241 acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
      301 ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata
      361 aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag
      421 gatacgttat gaaaccgacc accattgctt cacttcagaa atataagcag gataaaaagc
      481 gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaagggc
      541 ttaacgtcat gctggtaggc gactcgctgg gcatgaccgt tcagggccac gattccactc
      601 tgccagtaac cgttgctgat atcgcctacc acactgccgc cgtacgtcgc ggcgcaccaa
      661 actgcctgct attggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg
      721 aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt
      781 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
      841 gtttaacccc acagtcagtg aatattttcg gtggctacaa agtacagggg cgcggcaatg
      901 aagcgagcga tcgactactc agcgatgcat tagccttaga agccgcgggg gcacagctgc
      961 tggtgctgga atgcgtgccg gttgagctgg caaaacgtat taccgaagca ctggcgatcc
     1021 cggttattgg tattggtgca ggcaacgtca ctgacgggca aatcctcgtg atgcacgacg
     1081 cctttggtat taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg
     1141 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
     1201 gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct
     1261 gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac
     1321 tatgggtaat ctgcacaatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga
     1381 tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc
     1441 acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt
     1501 agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc acacttacgt
     1561 tgacgttcct ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg
     1621 cgtttcgact attgtcagca agctgttcaa cctggtccag ccggacatcg cctgcttcgg
     1681 tgaaaaagat tttcagcaac tggcgttgat ccgcaaaatg gttgccgata tgggcttcga
     1741 tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc taagttcccg
     1801 taacggttat ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca aagttttaag
     1861 ttcgattgct gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc
     1921 ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga
     1981 cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct
     2041 tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc
     2101 tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact
     2161 taaataaggt taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg
     2221 tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt
     2281 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     2341 atagacttcc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     2401 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     2461 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     2521 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     2581 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgcgc cagctccggc
     2641 gagtaaaaca tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     2701 aacagtatcg gcaccaccag cggcagctta tcgtggccag cctccagatg ccggtgcatg
     2761 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt
     2821 tcaatcacaa catgcagata accgggattg ccctgcattt gcatggaata gagcacgtcc
     2881 gtgctgtgac ctttcaggca ctcttcaatg aaactccccg actctaaatg aagcgtgttg
     2941 agatcacaaa gttcgcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
     3001 tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc
     3061 atcttccctg tcttccgtgt ggccttgcgt gaaggcagta tccagtcaag gcgtgctatc
     3121 gtcagccacc tctttattgt ttaccgagca gcgttcataa aaatatcaat tcgttacagg
     3181 cgttacatgg cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag
     3241 tagacactca tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc
     3301 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
     3361 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
     3421 accaggattt tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg
     3481 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
     3541 tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca
     3601 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg
     3661 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
     3721 tatccctgcg gctggttact caccagccgc gacatcttct ccagcgaatc cgttcttaag
     3781 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
     3841 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaagccg
     3901 ccaaacggat acgaaagata ccagacatgt ggattaaatt gcgccagagc gcggcgtgaa
     3961 cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgataa
     4021 ccatctaccc gatgcaaaaa atgggtatgt gactggaaat caaaaacatc gcgaatttcg
     4081 ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt
     4141 ttgatgcgtg aggtaacaat aaacaccgtc gccttcatgc catattgttt caacacagga
     4201 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca
     4261 gggagattga tcgtattctt cacgtaacct tccagctgag ccatgctcag tgtcgcgtat
     4321 cccctgtcgc gcagccaggc catttggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
     4381 gtatggcgaa aacgggtgtt ttcttcgtcg cgcagaatat ggtgataagt tagcaccggc
     4441 aggccattat cgggttgggc atccagcgcg ctgatatagg ccagtcgatc gccaatacgg
     4501 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
     4561 ttgtccgcca gtaccccaaa tggcgcactg cccacactcg gcgcgttgta gaccggcgta
     4621 tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct
     4681 tcaacttttt gtcgcccctg aactggctcg agatgccctt tatcgataaa ccctttacca
     4741 aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgtccg
     4801 gcccgaatat tccccacggt caccatcttg tcactaattt gcgcccagac cgcagcattt
     4861 tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc
     4921 agcaggagaa taacagcttg tttatacatg aaaaaataac cgagggtgaa acaacgcttt
     4981 tattgtagca aaagcgccat cagaatcgcg ggttaatact catacaaatt atgtttgagg
     5041 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt
     5101 tcgacaatac actcacgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt
     5161 tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat
     5221 aatgaagcca cgcgatacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca
     5281 ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaat
     5341 ccgcgccaga aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca
     5401 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
     5461 ctccttagcc gaaaatcagc ctaccctgac cattgttgat aaagggtgat agccgtcaaa
     5521 actcctctcc cccgaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
     5581 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
     5641 aggtagtgac cagcggcaca tcatttatac cgaggaagcc gtagcggaaa ccactgatca
     5701 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg
     5761 agtaaaagac cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
     5821 tgtcatcgaa cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg
     5881 ccgtgagcac caaggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
     5941 acagtgaaat tgccgtcacc agaatgccaa caaacagacc acgcgccacg ccaccgccga
     6001 catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac
     6061 gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcgttg gtgatcaccg
     6121 acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa
     6181 tacgcgaacc tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg
     6241 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
     6301 ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga
     6361 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
     6421 ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgtagcactt caacttccag
     6481 cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
     6541 tgcgagatcg agaataaagg tttccgattt cagcttcgcc agcagcgcct tcatcgaggt
     6601 attttccacc agctcaccat gttgaataat gccgatattg cggcacagca tttctgcttc
     6661 ttccaggtag tgtgtggtaa gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
     6721 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
     6781 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
     6841 taacatacgc gcacgttcgt tgcgttttcc ccataaatcg agctgcttaa gatatttttc
     6901 gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat tcaccacaat
     6961 ttgctgaacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg
     7021 tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
     7081 tttatttacc agagagctga taataccgat agcggtcgat ttcccggccc cgttcggccc
     7141 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac
     7201 gccgcctgga tagttttttt taagctgttg aagttccagt gcaatggtca taaattttta
     7261 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
     7321 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
     7381 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
     7441 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
     7501 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
     7561 aacctagtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
     7621 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgcc
     7681 gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
     7741 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
     7801 gaactgaacg tcatggaaca ggtgtataac ctgggccact ccaccattat gcaatcagcg
     7861 tggaaacgcg ggcagaaagt taccattcac ggctgggcct acagcattca cgacggcttg
     7921 ctgcgtgatc tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac
     7981 gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
     8041 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgcca atatacggca
     8101 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
     8161 agaaaccgat aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg
     8221 tacaaatcgc caacgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
     8281 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
     8341 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
     8401 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
     8461 taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct
     8521 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
     8581 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
     8641 gcaggttttg gcaccgcaac aaagcgcaag acgcgcagta taccagtaaa actattcatt
     8701 cgcggaatct gttaataaag ctcgaattgt gatgacggtc acacatgtta aaacctataa
     8761 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
     8821 cagcctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
     8881 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
     8941 ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgc gccgcgcttt
     9001 ggctatacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
     9061 acttctgggc gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc
     9121 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
     9181 tcgcactgct gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaga
     9241 tcaacggcac cttaagcgcc gatgccacac ctgccgaagc tatctccccc gttgccgatc
     9301 aggactggcc tgcgtatggc cgcaatcagg aaggtcaacg cttttcacca ctgaagcaaa
     9361 ttcacgccga taacgtccac aagctgaaag aagcctgggt gttccgtact ggcgatgtga
     9421 agcagccgaa cgatccgggt gaaatcacca atgaagtgac gccgattaaa gtgggcgata
     9481 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct tgatgccgcc agcggcaaag
     9541 agaaatggca ttacgatcct gagctgaaaa ccaacgagtc tttccagcac gtaacctgcc
     9601 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt
     9661 gcccgcgtcg tatcattctt ccagtgaatg atggtcgcct gattgcgatt aacgctgaaa
     9721 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
     9781 cagacaccaa accgggtctg tatgagccga cttctccgcc aattatcacc gataaaacca
     9841 tcgtgatggc cggttcagtc accgataact tctcaacccg cgaaacgtct ggcgtgatcc
     9901 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatccgggc gcgaaagatc
     9961 cgaacgcaat cccgtcagac gaacacacct ttacctttaa ctcaccaaac tcgtgggcac
    10021 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
    10081 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    10141 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    10201 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    10261 cgattattta cgctccggcg aaaaccggta acatttttgt gctcgatcgt cgtaatggcg
    10321 aactggtggt tccggccccg gaaaaaccgg ttccccaagg tgctgccaaa ggcgattacg
    10381 ttacccctac tcaaccattc tcggaactga gcttccgtcc gacgaaagat ttgagcggtg
    10441 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
    10501 tgcgctatga aggcattttc acgccgccat ctgaacaggg tacgctggtc ttcccgggta
    10561 acctggggat gttcgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg
    10621 ccaacccaat ggcactgccg tttgtttcga aactgctccc gcgtggtcct ggcaacccga
    10681 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
    10741 gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc atttggtctg ccatgtaaac
    10801 agccagcatg gggttatatc tcggcgctgg atctgaaaac taatgaagtg gtgtggaaga
    10861 aacgtattgg tacgccgcag gacagtatgc cgttcccgat gccggttccg gtgccgttca
    10921 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    10981 ccgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    11041 agggtcgttt accggcgggt ggtcaggcaa caccgatgac ctatgaagtg aatggcaagc
    11101 agtacgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    11161 ttgtggctta tgccctgccg gatgatgtga aataaccata atgcccggag agatccgggc
    11221 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
    11281 ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt
    11341 caccagcact tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg
    11401 agccgctggc ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa
    11461 cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc
    11521 cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
    11581 gaaatcgaac ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat
    11641 catctggctg tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg
    11701 catccccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc
    11761 ttccagcgaa ggtaacgcag gcaggctact taatgtgtca ggcaaagcac cggaggcact
    11821 aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggtg caatcgccat
    11881 ccccatctgg ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac
    11941 cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg
    12001 tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt
    12061 gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc
    12121 gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc
    12181 ggtcatcaca tccagttgat aatcaatctg cccgtcggcg ctaaatttct tatcctgaac
    12241 gatcaccgga acatcatcga taccccactg ttttggcagc attaatttca ggatctcgtc
    12301 atcttcaatc accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg
    12361 ttgatgtgga tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg
    12421 cttgccacct ggcggaataa ttccctgtgg gccgccgtcg acgtcacccg gtacttccag
    12481 cccgtgccag tgcaacgtcg tctcttccgc cagttggttg tagatatcaa ccgttaccgc
    12541 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt
    12601 tgcagttttc tcgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg
    12661 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc
    12721 acggctccac agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc
    12781 acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
    12841 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
    12901 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
    12961 aagacgtttt tcagaacagt gttattcggc agcctgatcg ccgtttgcgc aaacagttac
    13021 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg
    13081 aagaacgatt gtggttacca gaacttatct aacgggcaaa ttcgtcgcgc actggtcttt
    13141 ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc
    13201 ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaaatgcaaa
    13261 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
    13321 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
    13381 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
    13441 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
    13501 gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat
    13561 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    13621 ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    13681 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    13741 gcgggtgtcg tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac
    13801 gatccgcgct ttcaactagt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    13861 acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga aagcctgttc
    13921 acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    13981 caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
    14041 cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc
    14101 atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    14161 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg
    14221 gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga
    14281 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    14341 agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt
    14401 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    14461 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc
    14521 agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa
    14581 caccccggcc cactgccaga aacggtggtt gctcatctcg ataaaagtca tatttgcgta
    14641 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    14701 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    14761 gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga cattaacggt
    14821 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    14881 aaggcgctgt atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag
    14941 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    15001 accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat
    15061 tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat
    15121 ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa
    15181 cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc
    15241 cagcgaattc cagttgattg gcgcgaacca tcacctcttc agcgtccatc cacagggtgt
    15301 attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt
    15361 ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca
    15421 cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt
    15481 aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcatcgc gccattgatg
    15541 taattatcgc agccttaccg gagaaaaaca gcggaacggc gatagtttag aaataaaaaa
    15601 tgcccgggaa tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt
    15661 attacaccgc agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt
    15721 tgaagttcag ataacgataa gtatcaacgg cagttttatc tacttgcgca acgtaggtct
    15781 ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc agttctgcag
    15841 aagccaggaa gacattcgcg ccagtaccca aacggttcgg gaagttacgg gtagaggtgg
    15901 aaaccaccgt cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagcccg
    15961 ggatctcgat acgagccccg ctcttaccga agacgctgta gtagccttct tccgtcagtt
    16021 gcgcggcgtc catacgggtt ggcggtgcca cccacaggcg ggtcggcaac tgacctttgt
    16081 gcgcatccag cagtttacca gcggcacgga agtgaccgat gttagtcatg caagaaccga
    16141 taaatacttc gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcatccggat
    16201 cgttcggagc acacaggatt ggctctttaa tctccgccag atcgatgtcg atcactgccg
    16261 cgtattccgc atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc
    16321 cctgaatacg gcgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca
    16381 gcaggacgat gttggagctc aggtattcga tgatcggttc tttgttcagc ttgatagtac
    16441 aaccagcggc agaacgctcg gcggacgcat cggttagctc aaaggcctgc tcaactttca
    16501 gatccggcag accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct
    16561 tctcaacggt cagcagacct tgtttgatcg catacagcgg aatggcgtgt accagatcgc
    16621 gcagggtgat gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat
    16681 ccagcggcat tacgccagtt gcggcagcaa acgccaccag accagaaccc gccgggaaag
    16741 agataccgat cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca
    16801 gcatacggtt cagccaggag tgaatgacgc cgtcacccgg acgcagcgac acaccgccac
    16861 ggttcataat gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg
    16921 ccgcggtgtg gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt
    16981 ctttcagttc atcacgggtc atcgggccgg tggtgtcctg agagcctaca gaagtcattt
    17041 tcggttcgca gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt
    17101 tctgcgccag cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat
    17161 cactgtgcgg cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca
    17221 gcggaatacg gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg
    17281 tcgccagcag ttcgcctgtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa
    17341 tcacgtcgcc catgttcagg ttagagacgt tgacttcgat tggcagtgca cccgcgtctt
    17401 ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt
    17461 tgttcggcac atgtggaata tcatcgccca taaaccacag aacggagttc gtggcggatt
    17521 tacgcgatga acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt
    17581 gcagagcttc aatctgtttg atcgggccaa caacacctgg ctggtctggc tcaatacctt
    17641 cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat
    17701 ccggtgccgg agaaaggtca tcggtgttgg tttcgccagt gactttaaag acggtaacgg
    17761 tcagtttttc agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca
    17821 ttacctgctt cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat
    17881 cgaacatcag cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat
    17941 ccagcgcgtc gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga
    18001 tagctttttc cggagtcagc agaggggatt tagcttcgcc tttagcgata gcagccagga
    18061 agcctgcttt tacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat
    18121 ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agcgcggcca
    18181 tttggtttgc atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct
    18241 tacggtattc ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga
    18301 ttctcttcac gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg
    18361 cagaatagca ggattttgac gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt
    18421 ggctgaatcg ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg
    18481 catggtgata atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc
    18541 tctttatact cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct
    18601 tgagatccga attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg
    18661 cagtgccggg ctttgtgctg cctctgccgg gctatatata aaaagccgca cagtggaagc
    18721 gcctgtagaa ccgcaatcga cacaactggc tgtgtctgac gctgccgctg ttacgcttcc
    18781 tgcaacggtt tccgcacctc ccgtaatacc cgcagtcgtc aaatccgcat tcagcactgc
    18841 acaaatagat caatgggtcg cacccgtcgc gctgtatccc gacgccctac tttcgcaggt
    18901 gctgatggca tcaacctatc cgacaaacgt tgctcaagca gtgcaatggt cgcacgataa
    18961 tccacttaaa caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag
    19021 cgttaaatca ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg
    19081 ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca
    19141 gcgattgcgg caactggcgc aacaaaccgg ctcgctgaag tcatcaaccg aacagaaagt
    19201 tattaccaca acgaagaaag ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc
    19261 atccaatacc gttttaactg ccaaccccgt cattacagag ccggcaacaa ccgtcatttc
    19321 cattgagccc gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta
    19381 cgggaactgg gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc
    19441 gtttgttgac agctttgtgc gcggattcgg ctatagcatg ggcgttgcta ccacgtacgc
    19501 actattcagc agcatcgact gagatgacga cgatcatgac catcatcatc atgacgatga
    19561 tgattatcat caccacgatg gcggtcatcg tgacagtaat ggctggcaac acaacggcga
    19621 caacatcaat atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa
    19681 gaatatggca tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga
    19741 tatggcaaag cggtttcatc aaaccgatgt caacggcgga atgagtgcca cgcagctacc
    19801 tgctccaaca cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca
    19861 cgccgccccc gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga
    19921 agctgaacac tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac
    19981 ccagcagcaa aaggacgccg ctcgtcagcg ttatcagtcg gcctctcctg agcagcgcca
    20041 ggcagtccgc gagaaaatgc agactaaccc gcagatccag cagcgaaggg aggcggcgcg
    20101 ccagcgtatt cagtccgcct cgcctgagca gcgccaggcg gttcgcgaga aaatgcagac
    20161 taacccgcag aaccagcagc gaagagaggc agcgcgtgag cgtattcagt ctgcaactcc
    20221 cgaacagcgc caggtgttta aggaaaaagt acagcagcgc ccactgaacc aacagcaacg
    20281 tgataacgcc cgccagcgta tacaatcggc atcacctgaa caacgtcagg tttttcggga
    20341 gaaagttcag gagagccgcc cacaacgtct aaacgacagt aaccatactg tcaggctgaa
    20401 taacgagcaa cggtcagcag tacgcgaacg tctctctgaa cgtggagcaa gaagactgga
    20461 aaggtaaatt gcagacgtaa aaaaagcggc gtggttagcc gcttttttaa ttgccggatg
    20521 ttccggcaaa cgaaaaatta cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta
    20581 ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg
    20641 gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccaggccgat ttcacccagc
    20701 agctcgccgc cgttggtacc aacaatcgca ccaccgatca cacggtgaga ttctttgtcg
    20761 aaaatcagct tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc
    20821 cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga
    20881 cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag
    20941 tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt
    21001 gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc
    21061 agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg
    21121 tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc
    21181 ggttcagctg gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca
    21241 acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag
    21301 actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca
    21361 atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc
    21421 attaccagca ggcgttctgg tacttctttc agttccagcg cgtcagtgga gtcccagata
    21481 cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc
    21541 gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc
    21601 ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc
    21661 agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg
    21721 gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact
    21781 tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca
    21841 agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca
    21901 gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc
    21961 atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg
    22021 gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc
    22081 gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc
    22141 accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac
    22201 ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa
    22261 gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg
    22321 ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag
    22381 gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc
    22441 atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag
    22501 tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc
    22561 gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg
    22621 atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc
    22681 accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc
    22741 gaactattga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca
    22801 accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg
    22861 aacgcttcca gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc
    22921 cagttacggc tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg
    22981 atttcgccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg
    23041 ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg
    23101 tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca
    23161 aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc gttttcagca
    23221 aattcagatt tgccttccgc tttcgcagcc ggagcttcag cttttgctgc cggagccggc
    23281 gctgccgctt cctgtttcgc aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc
    23341 ataatcagcg aaccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg
    23401 cctgcaaacg gagccggaac ttccatagaa gctttatcgc cttctacggt aatcagtgac
    23461 tgttcagcgg caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca
    23521 ccgccgatat ccggaacgtt aacttctttc acgccagctg ctggtgcagg ggtcgctgcc
    23581 ggagtcgctt cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag
    23641 accataatca gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg
    23701 gtacctgcaa acggagccgg aacttccata gaagccttgt cgccttctac ggtgatcagc
    23761 gactgttcag cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg
    23821 tcgctgccga tatccggaac gttaacgtct tttgccgccg cagccgcggg tgctgctgcc
    23881 ggagctgctt ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg
    23941 gaatcgaaaa tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc
    24001 tctttaacga tacccgcctg cggagatgga acttccatag aggctttgtc gccttctacg
    24061 gtgatcagcg actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt
    24121 tcaacttcat cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct
    24181 cttacgccag acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag
    24241 caaccacttt cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gcaaccacga
    24301 cataagaagc atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga
    24361 agccatcagt acccagtacg cgatagtcgt cagccggtac gtaagtacgg acctgctcag
    24421 cgaacagttt catatagtcg gtagatgcca ctgccggcgc gtcgttcatc acctgagcga
    24481 tatacggaac gcgcggagtt tccagcggat gcagcatgtt ccagcgttca caatcctgac
    24541 catcacgcgc cagctcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat
    24601 ctttcgccag gatctctgcc gcttcacgga cgtgacgcag gatggaaccg gagcccagca
    24661 gctgaacttt acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac
    24721 cttcctcagc accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt
    24781 agtagtaaac gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga
    24841 cagcaacttc gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact
    24901 gaatgtggct gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg
    24961 aagtaccgcc gatcaggaag ccacgcgctt gctggtctcc agccgcccag cacagatcgc
    25021 cgatacgctg gaagccgaac atcgagtaat agatgtagaa tgggatcatc ggcagattgt
    25081 tcgtgctgta agaggtcgcc gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc
    25141 cttcctgcag aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct
    25201 gcggggtgta ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca
    25261 taccgaaagt acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt
    25321 tcttcagcat cacgttcaga gcacgaacga aagcgatagt ggtagagagc tctttgctct
    25381 gctcttccag cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt
    25441 tcggctgacg gcttggcaga taaccgtgca gtttctgacg ctgagcgtgc agataggtat
    25501 gctcttcaga accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca
    25561 ccggcacatt gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct
    25621 ggtgcgcgat gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag
    25681 caaggattac tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt
    25741 tcttcggatc gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa
    25801 ccagtgctgc ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt
    25861 tcgatttgaa ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac
    25921 cgctggtatc tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt
    25981 tccagccagc accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga
    26041 ccgggccgtc aagacgctgc aggttacagt tgataacgaa aaccaggtta tccagttttt
    26101 cacgggtagc gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga
    26161 ggaacgcgta aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca
    26221 ggaatttagc ctggtaaata gcaccaatcg gacccagacc catagatacg gtcgggaact
    26281 gccagaattc cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt
    26341 cctgacggaa gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgggcgt
    26401 aaacgcccgg ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc
    26461 gtgcacggaa gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg
    26521 ccatgtggcc gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt
    26581 tccagcggat agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt
    26641 gttcttcaac ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta
    26701 caccgccttt gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac
    26761 cttcttcacg gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca
    26821 cgtcatttgg gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt
    26881 tctggaacct gtcccattga actctcgcca gaaagctcaa taagacaggt tctacgttta
    26941 gttaccgcgc ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa
    27001 aatcactaat tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca
    27061 cggcttctgt cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc
    27121 gcttcttccg gcttaccggc cataatcgct tcaaatatgc gggtgcggtg actactcacc
    27181 agcggcagca tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc
    27241 aacatcggct ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg
    27301 acggcaatct gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc
    27361 gccagctcta tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc
    27421 agcgcggcgt aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac
    27481 tgtgattcag gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg
    27541 ctgctctgga caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga
    27601 cgttgaatcg cctcacgcaa ggagggacgg gagacgtcga actgttttgc cagttcgcgt
    27661 tccggtggga gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc
    27721 tgctcaatca catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt
    27781 cttaagccac ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta
    27841 acaaagtatt caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt
    27901 ttaacaactt gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac
    27961 gtaggaaagg gattttttaa cgattattaa actataaaaa tccgaattga accgattcac
    28021 ttaccaattt tttgattttt aagtcaataa aaacgaattt aaattcactc tgcatattga
    28081 gaggggttga ggctgagctt tacaaacggt ttctttttaa gcaactcatc ttcaaccatg
    28141 cataaagcgg gtgcattcgc tgccgcatac cattattctt gatctgacgg aagtcttttt
    28201 gtaacaattc aaacttcctt gatgtaaata aatcaataca acaaacggaa ttgcaaactt
    28261 acacacgcat cactgcgtag atcaaaaaaa caatcaccgc acgaggtttc atgatggaag
    28321 gtcaacagca cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg
    28381 cgctgggtgg cgcgataggg acagggctat tcctaggtag cgcctccgta atacagtccg
    28441 cagggccagg gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc
    28501 gtcagctggg tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt
    28561 ataaatactg gggcagtttt gccggtttcg cctctggctg gaactactgg gtactgtacg
    28621 ttttagttgc catggcagag ctgactgccg tgggtaaata cattcagttc tggtatccgg
    28681 aaatcccaac ctgggtttct gccgccgtat tctttgtggt gattaacgcc atcaacctga
    28741 ccaacgttaa agtgtttggc gagatggagt tctggtttgc cattatcaaa gttattgcgg
    28801 tagtagcgat gatcatcttc ggcggctggc tgctgttcag tggcaacggc ggcccgcagg
    28861 cgagcgttag caacctgtgg gatcaaggtg gtttcctgcc gcacggcttc accggactgg
    28921 tgatgatgat ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg
    28981 cagcagaagc tgataacccg gagcaaagta tcccgaaagc aactaaccag gttatctacc
    29041 gcatcctgat tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc
    29101 gcgttaccgc cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg
    29161 tggcgaatgc gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg
    29221 tatattgcaa cagccgtatg ctgtttggtc tggcacaaca ggggaacgcg ccaaaagcgc
    29281 tggcgtctgt cgataaacgc ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta
    29341 cagcattgtg cgtattgatt aactatcttg ctccggaatc cgcatttggc ctgttaatgg
    29401 cactggtggt atccgcactg gtgatcaact ggacgatgat cagtctggcg catatgaagt
    29461 tccgtcgcgc caagcaggaa caaggcgtgg taactcgctt ccctgctctg ctttatccgc
    29521 tgggtaactg gatctgcctg ctgtttatgg cggcggtact ggtgattatg ctgatgaccc
    29581 caggaatggc gatttcggta tacctgatcc cggtatggct gatcgtgtta ggtatcggct
    29641 atctgtttaa agagaaaacc gccaaagccg taaaagcgca ttaatctctc tacgccctca
    29701 cccgtacagg gtgagggcaa taatctctac accaacgccc cgtaaatcgt cagtagcgca
    29761 atcaccacca cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc
    29821 tccaccttat cgacatgcgg ttcacgcgcc agagagaact gcgccagacg cgttaatacc
    29881 tgatattgcg aagtatggaa atcacccagc gaagcaaacc aggccggtaa cgctttctca
    29941 ccatgaccga tcaaggcata taccacaccc gcaagacgaa ccggcaccca atccagtaca
    30001 tgaagcactg catcaatgcc ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc
    30061 cagtattgcc atgcacgcaa gaacgcatac cccatcagcg taacgggtcc ccaggttccc
    30121 cccacaatca gccagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca
    30181 ttttgcagct cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg
    30241 gtgagttcgc cagccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga
    30301 taagcatgat aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc
    30361 cacaccagca gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa
    30421 gtcacaccca tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc
    30481 cgccgaaaga acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg
    30541 cgctcgaaaa ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca
    30601 gcgcacgaaa ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa
    30661 tatcacaatg gcccgtcatg ttattagcga tatcgggata gcgatctatc agtgcccgcg
    30721 taaccgccgc aagctgttga tactgcgcat cggtatacgc cagagtatcg gtgccttcaa
    30781 gctcaatccc aatagaaaaa tcattgcagc gttcacgccc ctgatactga gagactcccg
    30841 catgccaggc acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca
    30901 aacagtgagc ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat
    30961 caatagttcc agtgaataat gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc
    31021 taatattgtg caccaccagc agggtgggtg tttcgtcatc caggcggcaa tcgtaatgtg
    31081 gtgagggaac gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc
    31141 ttatatgtgg tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgttagc
    31201 tatctggagt tttaacatgc cgcctcgccg ctacaaccct gacacccgac gtgacgagct
    31261 gctggaacgc attaatctcg atatccccgg cgcggtggcc caggcgctgc gggaagattt
    31321 aggcggaaca gtcgatgcca acaatgacat tacggcaaaa cttttaccgg aaaattctcg
    31381 ctctcatgcc acggtgatca cccgcgagaa tggcgtattt tgtggcaaac gctgggttga
    31441 agaggtgttt attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg
    31501 cgatgtcatc aacgccaatc aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt
    31561 aacgggcgaa cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt
    31621 acgccactat gtcgaattac tggaaggcac caacacgcag ttgttggata cgcgcaaaac
    31681 cttacctggc ctgcgttcag ctctgaaata cgcggtactt tgcggcggcg gagcgaatca
    31741 ccgtctgggg ctttctgatg ccttcctgat caaagaaaac catattattg cctccggctc
    31801 agtgcgccag gcggtcgaaa aagcgtcctg gctgcatccg gatgcgccag tagaagtcga
    31861 agtagagaat ctggaagaac ttgatgaggc cctgaaagcc ggagccgata tcatcatgct
    31921 ggataacttc gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct
    31981 actggaagtg tctggcaacg tcactgacaa aacactgcgc gaatttgccg aaacgggcgt
    32041 ggactttatc tccgtcggtg cgctaactaa acacgtacaa gcactcgacc tttcaatgcg
    32101 ttttcgctaa ttgctgacag atttatccgc tccatcgcgg gcggataatc tccccttccc
    32161 caactttctt cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc
    32221 tcggaatctg ctttcccgtt tcgttttttg cccctcgcct gtgctgatgg cgtttgccaa
    32281 agtagcacca accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta
    32341 tcgaactgat ggtggttatt ggcatcattg ccattttaag ctccattggt attcccgctt
    32401 atcaaaacta cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc
    32461 gtaccgccgt agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca
    32521 gcaatggcat tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg
    32581 gcgtggtgtc gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacgccag
    32641 gttgggataa cgcaaacggc gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca
    32701 gcgcattgca gcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg
    32761 caatgaatat tccacagctc acggccctgt gcctgcgtta tcagggagtc ttgctggatg
    32821 ccagcgaaga agtggttcat gttgcggtgg tcgatgcccc ctcacatgag ttgctggacg
    32881 cattgcattt cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg
    32941 aaggtcacgc cagtcgcaca caacagacat tacccgtagc tgttcaggag aagcatcagc
    33001 ccaaagcaga attgctgact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata
    33061 ttcatatcga accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc
    33121 atcctttacc ggatgtttca ccggatgccg gagtcgcatt aacagccaga ttaaaagtgc
    33181 tgggaaacct ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac
    33241 tggcaggaaa caccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg
    33301 tggtattaag gttgttacag caggtgagcc aggcactgga tgttaacacg ctgggaatgc
    33361 agccgttaca actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg
    33421 taactggccc tacaggcagt ggcaaaacgg tcacctggtg ttgtccacgc tacacaccaa
    33481 ttccaccact gaaacgctgg tacgtttaca gcaaatgggg gtcgcccgct ggatgctatc
    33541 atcggcgctt acgctggtaa tagcccagcg tctggtacgt aaactttgcc cacattgtcg
    33601 ccgacagcaa ggggagccca tccatattcc agacactgta tggccgtcgc cgctgcccca
    33661 ctggcaggca cccggttgtg tacattgcta ccacggtttt tatggtcgta ccgccttgtt
    33721 tgaagtcctg cccattacac cagccatacg ccagcttatt tccgctaata ctgaagttga
    33781 atcgctggaa acgcacgccc gacaggcggg tatgcgtacg ctttttgaaa acggctgcct
    33841 ggccgtggag caaggcttaa ccacctttga agagttaatc cgcgtactgg ggatgccgca
    33901 tggcaagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg
    33961 ggatgctatg ggcagagagc cgtactttac tgcttatggc actacagcaa cagatggtta
    34021 ccccactaag cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg
    34081 cggaagtcat tcatcaactg gcgacgctac ttaaagccgg gttaacgctt tctgaagggc
    34141 tggctctgct ggcggaacag catcccagta agcaatggca agcgttgctg caatcgctgg
    34201 cgcacgatct cgaacagggc attgcttttt ccaatgcctt attaccctgg tcagaggcat
    34261 ttccgccgct ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat
    34321 gctgctttga actggcgcgt cagcaaaaat cccagcgtca gttgaccgac aaagtgaaat
    34381 cagcgttgcg ttatcccatc atcattttag cgatggcaat catggtggtt gtggcaatgc
    34441 tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc ccactaccgg
    34501 cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggggc tggctgctgg
    34561 tgttgttcgg ctttctgctg gcgatagcca ataagttgct gatgcgccgc ccgacctggc
    34621 ttatcgcgcg gcagaaattg ctgttacgca tcccgattat gggttcactg atgaggggac
    34681 aaaaactcac gcagatcttt acgattctgg cgctgacaca aagtgcaggc attacttttt
    34741 tacagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga
    34801 cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgcta aaaaatgccg
    34861 gtgagtttag cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctcgctgg
    34921 acctcatgtt agacaacctc gcccatcatc atcgggataa cacaatggcg ctggcggata
    34981 acctcgcagc cttactggaa ccggcgttgc tgatcataac gggaggaatt atcggtacgc
    35041 tggtggtggc aatgtatctg ccaattttcc atttaggcga tgcgatgagt gggatgggat
    35101 aacgctggcg tgttgctcca cgccagcgtt gggagattac aggttgttga agatgcggtt
    35161 ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc agctctttca ggcgtgaagc
    35221 cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac
    35281 ctggcctcgc agcggcagct taacggtttt accttctgct gcgcggtatt ccgcaacgcc
    35341 gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca taaatttctc
    35401 gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca tgccgccaag
    35461 catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca tcgcccggcg tggtacagcc
    35521 accgtcgctg acgatcattc cgcccagacc gtgcgccgca tcggcacatt cgattaccgc
    35581 agaaagctgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc
    35641 aatgccaact ttaacgatat cagcaccgga gaggataagc tcctcacaca tttcaccagt
    35701 cactacgttg ccagcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa
    35761 ctgcacgaag tgttcggaat aaccattcgc cacatcaata caaacgaagt ttaatgccgg
    35821 gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtgc cggtagaaac
    35881 catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac
    35941 agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa gccagcgcag aggccataga
    36001 aaatgtgcct acggtgtcca tatttgcggc gataatcggc acgccggacc agctctgacc
    36061 tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga
    36121 gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat
    36181 gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt gacgctatca
    36241 tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca
    36301 taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcactttcg cgcgctcatt
    36361 tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgggg tatatagttg
    36421 ccttaacggg aggcattggc agtggtaaga gtaccgttgc caatgcgttt gctgatctcg
    36481 gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg
    36541 cgctacatgc cattgctgat cactttggcg aaaacatgat tgctgctgat ggaacattgc
    36601 agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg
    36661 acctgctgca tccgctgatt cagcaagaga cgcaacacca gatccagcaa gcaacttccc
    36721 cctatgtact gtgggttgtg ccattgctgg tagaaaactc actgtataaa aaagcgaatc
    36781 gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg
    36841 atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc
    36901 ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg
    36961 ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac
    37021 cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct
    37081 gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg
    37141 cgcgctgcat ttcttccgta atgtcagtga attactggat gttttcgagc gcggcgaagt
    37201 ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt aaactccaga cctggattgg
    37261 cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa aagcggcggt
    37321 gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc
    37381 tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt tacctacatt
    37441 gcacatttgg ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc
    37501 cagcctgaac ccgctcaccc aggcacttac catggtgctg gatttaattc gccagtcggc
    37561 ccccttccgt aaacaaacca gcctgaatgg tttttatcag gataacggtg gcgatgccga
    37621 cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggacataa
    37681 gagccgtttt gccattcgtt ttatgccgct ggacactgaa aacggacagg taccggaacg
    37741 tctggatttc gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa
    37801 ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg
    37861 ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc
    37921 cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattt
    37981 gtctgatgcc tcacgtaggc ctgataagac gcggacagcg tcgcatcagg catcttgtgc
    38041 aaatgtcgga tgcggcgtga acgccttatc cgacctacag acgcttaagc ttcgcaatta
    38101 ccggttcatt ggctggcgga aaatcatcgg cattaagacc gaccagcgac atccactcac
    38161 cgggttgccc ttctttaccc cacggcaccc cttcccaact ttcgaccagc cagaaccaca
    38221 gagttatatg cctgtccggg aattcatatt ccagtttttc aaatagcgaa aaatgttgtg
    38281 gggtaatccc gacctcttcc tgaagttcac gcaccaccgc ctgttccggc gtttcgccca
    38341 tttcaatttt accgccggga aactccagtt tattcgccat gtgcgcatct gctgcgcgac
    38401 gtgttataaa gatttcattg ttctcgttgc gaataatacc taccgcaatt tgcagctttt
    38461 tcattgtctt aaacctataa aaaaggcgca gaatcctgcg ccttttactt caactgttag
    38521 cttttattgc aggcgaccat ggcactgctt gtatttttta ccagaaccgc acgggcaagg
    38581 atcgttacgt cctactttgc gttcaccggt ttgcgccgcc agtgcagctg cggctgcaga
    38641 gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa cgctcggctt ccatacgacg
    38701 ctgttgttcc agctcctcaa cctcttcagg catacgtacc tgaactttgc tcagcgtact
    38761 gataacttca tatttcaacg actccagcat cgctgcaaac atggagaacg attcacgttt
    38821 gtattcctgc ttcggatctt tctgtgcata gccacgcagg tggataccct gacgcagata
    38881 gtccatcgct gccaggtgct ctttccacag ggagtcaagc gtttgcagca tgacgccttt
    38941 ctcgaagtga cgcatcatct cagcaccaac cacttcttct ttacgctgat acacttcgat
    39001 ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc agttctggtt ctttatccag
    39061 ccactcggca attggtaaat cgaggtcgaa atcgttcttc agacgttcct gcagccccgg
    39121 aatatcccac atttcttcca gcgactgtgg cggaatgtag gcatcaatgg tggctttgaa
    39181 cacatcttca cgaatgctgt tgatggtttc gctcacatcg ctgacatcca gcagttcgtt
    39241 acgctgggag taaatggcgc gacgctgatc gttagccacg tcatcatatt ccagcagttg
    39301 cttacgaatg tcgaagttac gactttcaac tttacgctgg gcgttggcaa tcgctttagt
    39361 cacccacggg tgttcaatgg cttcgcctgg cttcataccc agtttacgca tcatgccgga
    39421 tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt agaaacggga
    39481 agaaccagca tccccctgac gaccagaacg accgcgcaac tggttatcga tacgacggga
    39541 ttcgtgacgc tcggtaccga tgatatgcag gccacctgct tccagtaccg catcgtgacg
    39601 tacctgccag tcggctttaa ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc
    39661 aacttctgcc tgccagctac ctccgagcac aatatctgta ccacgacccg ccatgttggt
    39721 cgcgatagtc accgcagccg gataacctgc ctgagcaaca atcgccgctt cgttggcgtg
    39781 gaatttggcg ttcaggacat tgtgcttaat accggctttg gtcagttcgt ttgacaccag
    39841 ctccgatttt tcgatggaga tagtacccac cagcaccggc tggcctttcg cagtacgttc
    39901 tttgatatct tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg
    39961 cagatcttta cgaatcattg gacggttggt cggaacaacg acggtatcca gtttgtagat
    40021 ggagctaaat tcgaaagctt cggtatcagc agtaccggtc atccccgcca gtttttcata
    40081 cagacggaag tagttctgga aggtgatcga agccagcgtt tggttttcgt tctggatctg
    40141 cacaccttct ttcgcttcca cagcctggtg cagaccatcg gaccagcgtc ggccctgcat
    40201 ggtacgaccg gtgtgttcgt caacgatgat aacttcacca tctttaacga tgtagtcgac
    40261 gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat
    40321 gttggccgga gagtacagag actccccttc atccatgatg ccctctttca ccagcagttc
    40381 ttcaatcagc accagaccac gttcggtcag gttcacctgg cgagattttt cgtccaccga
    40441 gaagtggcct tcgccctgga aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg
    40501 aataatttta ttcacgcgtt tatacatttc cgagctgtct tctgccgggc cggaaatgat
    40561 cagcggtgta cgcgcttcat cgatcaggat ggagtccact tcgtccacca gcgcatagtg
    40621 cagtttacgc tgtacacgtt cttcagggct gaacgccatg ttgtcgcgca ggtagtcaaa
    40681 gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc gctttgccgg
    40741 tgctggcatg cccggcaggt tgataccaac agtcaggcca aggaattcaa acagcggacg
    40801 gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt gcacgccttt
    40861 accggttagt gcgttcaggt aagcaggcag cgttgcggtc agggttttac cttcaccggt
    40921 acgcatttcg gcgatgcagc gttcgttaag aaccataccg ccgagtaact gaacgtcgaa
    40981 gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc acggcgaaag cttccgggat
    41041 cagattttcc agcacttcgc ctttttccag acgcgcacga aactctgcgg ttttcccttt
    41101 cagttcttca tcggagagtt tttccatctc cggttccatg gcattgatga tgttgaccac
    41161 tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg aaaactttag ttaacaattt
    41221 gattagcata ataaaatctc aaacgccccg cgttacggag ttaataaaat gaagtaaagg
    41281 tttattgttg ttaggtgagg cgttgagggc cagcacggat gccttgcgcc tggcttatcc
    41341 agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg cgataaccct
    41401 tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt aatgcaagat
    41461 gttgcgcctg aagaggcaaa gattcttcag caacgggcag tgtttgcggt gccattgcga
    41521 aagaaagatg acggattacc gtacgaatag catgttgatg ccagtaatca acggaatagt
    41581 tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag ttaactttgg
    41641 ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg gcggcgttgc
    41701 tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa taagagatgc ggccagaagt
    41761 agcgtttacc aaactgtcgc cagcgcgtca gtattccact cacgttattg ccatcccgat
    41821 cgaattcaaa cgtatttagc gcacaaatct tatcattaaa gcctgaagtc cacagcacga
    41881 atgaagataa gttgtgcgta aaagtgctta agaaaacagc gttcgcaccg ttttttatag
    41941 ctcttataaa attcaaaaaa aaacgactgg ttcacctggc cggagagagt gccagatttg
    42001 ccagtcgaat tttatacgac agtataaatg tcgttatgcc agtacagctg aaggcgcttt
    42061 gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt
    42121 cgccaggaca gcctgcagca gtttgttatt cagtgcatga ccggatttat aagcggtaaa
    42181 tgcaccaata atattgtgac cacacatgaa caagtcaccg atcgcatcga gcattttgtg
    42241 acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac
    42301 aacgatggca caatcgaagc tgccgcccag gcacaaacca cgggactgca gatattcgat
    42361 atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg catcagcgga
    42421 gaagttcatc gcatagcgct ggttgctgga atcaatagcc ggatggttaa aatcgatggt
    42481 gaaatccagc gaaaaaccat tgtacggctt aaattcagcc cacttatcgc catcttcgac
    42541 acgaacagtc tctttgatgc gaacaaattt tttggcgcag ttcaactcgt cgataccggc
    42601 gtcaagcagc aggtatacaa acggagcggc gctgccgtcc atgatcggga tttccggcgc
    42661 gttaacttcg ataacaatgt tatcgatgcc caggcccgcg agagcagcat tgaggtgctc
    42721 tacggttgaa atccgtacat catgctcgtt gaccagacac gtacagagca tggtatcacg
    42781 cacagatttg gcatcggccg ggaaatctac cggtggattc aagtcggtgc gacgatagat
    42841 gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg actttcttgc cggtatgtaa
    42901 accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca tcgtattatc
    42961 tcgccaaatt acctatccaa ccgaagtgta ctatacattc ggcgggccag tttagcacaa
    43021 agagcctcga aacccaaatt ccagtcaatt cttaatcagc ttgcttacgt aggaatgctg
    43081 ggatatccag ataatccggc tctttcgcag tttgcggcgc attgtcattc actactttag
    43141 caaccggctt ctgctcctgg gtcagcggag ccatcccatg ctgctggtag cgatccatca
    43201 ctggctgctg aacctgctta ttggtcacca gagtgatttc aggacgtttg tccatgccga
    43261 tacctgtcgc aacaacggtt acgcgcagct cgtcattcat atccgggtca agagaagtac
    43321 cgataaccac agtcgcgttg tcggaagcaa atgcacggat ggtgttacct accgtttcga
    43381 actcatccag acgcaggtcg aagcccgccg tgatgttaac cagcacgccg cgcgcgccag
    43441 acaggtcgat atcttccagc agcggagaag agatagccat ttcagcagct tcttccgcac
    43501 ggtcttcacc gctcgccacg ccagaaccca tcattgcgta gcccatttca gacattacgg
    43561 tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg agtaatcagt tcggcgatac
    43621 cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc agggagatac
    43681 cgcggcccag aactttcagc agtttgtcgt tcgggatagt gatcagagag tccacatgct
    43741 tggacagttc agtgatcccc tgctcggcga atgccatacg cttcttgcct tcaaagttga
    43801 aaggcttagt gacgacagca acggtcagga tacccaaatc ttttgccact tcagcgacga
    43861 ctggtgctgc acctgtaccg gtaccaccac ccatacccgc agcaataaag accatgtctg
    43921 caccttccag cgccgcacgc aatgcatcgc gatcctcatc agccgcattg cggccaactt
    43981 ctggattagc gccagcgccc agtcctttgg tgataccgct accgatttga atcgtctgtc
    44041 caaccgctgt tttacgcagc gcttgtgcat cggtatttac cgcgaagaat tcaacacctt
    44101 caatacgctc gcgcaccatg tgttcaacag cattaccgcc gccgccgccg acgccgatga
    44161 ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa catagtttct ctccgatttg
    44221 tgcctgtcgc ctgaggccgt aatcatcgtc ggcctcataa aaattaaaac tcttttcgca
    44281 gccaactatt gagtcgcttg atccacgagc caactgatgc tgtaacacgt ttttctactt
    44341 cagcttcacc gttaagatgt gactctttcc catagtgaag caatcccacc gccgtcgaat
    44401 aatacggctc ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca
    44461 cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac
    44521 cggttaatac aatgcctgcc gccaggtgat gtttaacccc ttgttggcga agcttttcct
    44581 gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc ggtatagcgc ggctcgatca
    44641 cctctgccag ggtctgacgt tgcagactcc gcggcggacg accgcctacg ctcggcactt
    44701 ccacgctctc atcttttcca acgatggaac ccagcgcaca accgtggcga actttaatcg
    44761 cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat atcactggtc acgacattgc
    44821 cagcataagg aattaccttt gtgtggcgca atgccccacc ggtataaacg gcgatatcca
    44881 ttgtaccacc accgatatcg acgacgcaga cacccagttc acgttcatct tccgtcaata
    44941 ccgaataact tgatgccagt ccggcaaata tcagttggtc aactttcagc ccacaacgtt
    45001 caaccgcttt gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg tgcacttttg
    45061 cctgcatccg cacacccgaa agtcctaccg gattcttgat cccttcctga tagtcaatcg
    45121 catactcttg cgggatcaca tgcagcacac gatgctcatc gcgcacacgc accgatttcg
    45181 cggtatggac gacgttttcc acatcttctt gcgtcacttc ttcttcagaa ataggcacca
    45241 taccaatttc attctggcag ctgatgtgct taccagaaag cgccagatat accgaagaga
    45301 tctgacaatc tgccatcaat tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg
    45361 attcgaggtc gttcaccccg cctttatcca taccacgtga cgggcagctg cccacgccaa
    45421 tgatattgac cataccgtcg ggcagaactt cccctactaa agcggcaacc ttcgcggtac
    45481 caatttccag tcctactacc agttttctgt ccgtcgcctt gatcattgtt gttctgcctg
    45541 tgcctgattt tgttgctgag tagattcctc tggcggcaag ggcgcccagc ctactgccgc
    45601 tccagagtca taacgcaaat caacgtagct aatccgtttg ccatcggttt gcgcctgctg
    45661 ctgtaaaacc ggataaagtt ctacaaagcg aaccaaacgt ttcatcgtat cgccccggcc
    45721 aagattgagc ttaatatcgt tattcagcgt caactgccag gaacgccgcg cggtcatcgc
    45781 cgcttccttc agagtaaatc tgtcctttgc cagcatctgc cccatttcgc gatagccctg
    45841 caacacttca ttagcgctgc cttccgggcc atacagcatt ggaagcacct gcttgctggt
    45901 gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct accatgtgtt gatcattcca
    45961 ccgcgcaatc ggcacatatt caaccagatg aatcttcaat tcatcaggcc actgctttct
    46021 gacgctcacc tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga tgatgttgac
    46081 atcctgggtc ataaaggtac ccggctcacc caatgccagg atcgactgcc ggatatcgtc
    46141 attacgcgtg taatggcgtt caccggtcaa caccagcttt gagagcggca ggcgttgcgc
    46201 atcttccatc cagctcaaca cgacccagcc gctcaccaac actgtcgtta aaacggtcag
    46261 caggaaaagg atccccgcca gacgcgttcc attattgcgg cgagaagaaa cctcttcttc
    46321 gctgtttcgc gtgttcagag cagcctgcga catattagtc cgccagttcc agaattcgta
    46381 ctaccaactg cgagaagctc atacctgcct gacgtgccgc catcggcacc aggctgtggc
    46441 tggtcatacc cggtgaggta ttggcttcca gcagataaaa ctgtccatca ctgtccagca
    46501 taacgtcaat acgtccccat cctttgcaac ctaacgtcgt ccatgctttc agcactaatg
    46561 cctgcaaatt ggcctcttgt gacgcttcca gacctgcggg gcagaaatac tgtgtctcat
    46621 cagagagata cttcgcctca taatcataga aggttccgga cggttgaata cgtattgacg
    46681 gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg cccacttagc catttttcaa
    46741 tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga gcattttctg
    46801 ctactacttt tgacattccc acactggaac cttcgcggct cggcttaacg ataaccggca
    46861 aacccagagc agaaatttct gctaactgct tatcgctcag gcctttttca aactctgcgc
    46921 gggttaacgc tacccacggc gcgaccggta aaccggcacc ttgccacaga agtttgctgc
    46981 gtagtttatc tattgaaagc gcagatgcca tcactccgct gccggtataa ggcaagccca
    47041 tcagctcgag catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa
    47101 acactttctg aaaacccatc gacttcagtt gcgtcacgtc gacttctttc gggtcgacag
    47161 gatacgcatc aataccgcct tcacgcagcc cggctaacac cgctgcgcca gaattcagag
    47221 aaacttcccg ctcagcggaa gtcccgccca acaggaccgc gattttatca gtcatgttgt
    47281 tcttcctccg gagtttgcgg cttcagtttg atttcagcta aagaacgggc aatttttccg
    47341 atattaccag ccccctgaac gagaatcagg tcgttaccgg ttaataccgg tgccagcatc
    47401 tcggctacct gcgcagggtc cggcaccaga atgggatcaa ttttcccacg tccacgaatt
    47461 gtgcgacaca gcgaacggct gtccgctccc ggaattggcg cttcgccagc cggatacact
    47521 tccagcatca acagggtatc aacctgcgtc agcacattgg cgaaatcatc atacaggtcg
    47581 cgcgtacggg taaaacggtg cggctgaaac agcattacca ggtttttatc cggccagcct
    47641 gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta gtcatcgacc
    47701 agcattgccg taccgctttt accattcact ggctccagcg ggaattcacc gaggaaatca
    47761 aaacggcgac cagtcccctg gaagctctcc agcgcacgca aaatagcctc gtcgtcaatt
    47821 ccctcttccg tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg acggcctggc
    47881 gcattcaggg tgacgcgcat cggctctttg tcctgacgca gcagcgtaaa gtgcccctgc
    47941 gggccaatct gctgataatc ttctacacgc acgtcggcat cttcgctgaa gccgtaagtt
    48001 gtggtctgac gtcccacacg cggtaacaat tcgcggatca ccggatcatc aacacacatc
    48061 accgcacgac cgtaaaaagg caggttgtgc agaaaattaa taaaggtctg ttttaaattc
    48121 tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga tattggtgac aatcgccacc
    48181 atcggttgca gatgcaggaa cgatgcatca ctctcatctg cttcggcaat caggtaccga
    48241 ccatgcccca aacgcgcatg aacccccgcc gcttttacca gcccgccgtt aacgaaggtt
    48301 gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg cggtagttgt cgttttgccg
    48361 tgcgttcctg caatggcgat gccatgacga aaacgcatta actcagccag catttcggca
    48421 cgacggatca ccggaatacg cgcttcatga gctgcgacaa tttccgggtt gtcggcagaa
    48481 atggcgctgg aaacaacgac cacgctggca tcacgtacgt tttccgggcg atggttgaaa
    48541 taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg gatttggcgc taaatcggaa
    48601 ccactgatct gataaccttc attggccaga acttcggcaa taccgcccat accggcacca
    48661 ccaatgccga caaaatgtat gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt
    48721 tttgccaatt gttgtgtatt cattctttac gccattaact tcttaaattc atacgatgca
    48781 aaaggcatcg ctacaattac gcccgggcag cccggctcac ttcatttgcc actcgctcgg
    48841 tggcatccgg aatggatgca gcgcgggcgc gttctgccat ggttaataag gtttctcgcg
    48901 accacccggc cagggtgttg gcgacagcat ccacgctaag ctgtggctgc tcaataattt
    48961 tggctgcgcc cgctttttcc agcggtagcg cattccagta ttgctggcgg tctttatgtt
    49021 gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat ttcactcaca gttaaagcac
    49081 cggagcggca aacgacgaca tccgcccacg catacgccgc cgccatatca tcaataaatt
    49141 cagtcacttt atgctgcggt tgccccgctt cggcatacgc ctgttcaacg gattgttgcg
    49201 aacctttgcc gctctgatgc cagatagtga ctgaatcacc cagtttcgca gcaacctgcg
    49261 gcattgtctg gttaagaatg cgtgcgccct gggaaccacc cactaccaac acacgaaccg
    49321 gaccttcacg tccagccaaa cgttgctgcg gcaacggcag cgccagcaca tcggtacgca
    49381 ccgggttacc caccacttcc gcattaggga aagcgcctgg aaacgcctgc atcactttgg
    49441 tagcaatctt cgccagccat ttattggtta agcccgcaat accgttttgt tcatgaagta
    49501 caaccggaat gcctaacgac cacgcggcca gaccacctgg acctgataca tagccgccca
    49561 taccgagtac cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga cgccaggcgt
    49621 tgaagatacg cagcggcgca gctatcagtg cttttatacc ttttccacgc aaaccagaga
    49681 tacgaatgaa atcaatttcg atgccatgtt ttggcactaa gtccgcttcc atacggtcgg
    49741 cagtccccag ccagcgaact tgccaaccct gagccattag atagtgcgca accgccagtc
    49801 ccgggaatac atgtccaccg gttccgcctg ccatcaccat taatcgcttt ccttgaccac
    49861 tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat aatcaatacg
    49921 caacagcatc atgatggctg tcgacataat cagtaagctc gaaccaccgt aactgattag
    49981 cggcaatgtc agacctttgg tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc
    50041 ctggaagcta aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat
    50101 ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg acgaagaata ccattaaaag
    50161 tgctagtacc acaccgacat aacctaattc ttcgccgata atggcgaaaa taaagtcagt
    50221 gtgcgcttcc ggcagatact ccagtttttg taccgaatta cctaaacctt gcccccaaag
    50281 ttcgccgcga ccaaacgcca tcagcgattg cgttaactga tagccgctgc caaagggatc
    50341 ttcccacggg ttccagaatg cggtaacacg gcggatacgg tacggttcgg cgaggatcag
    50401 caacacaacc gctgaaatgc ccataccgat aatggcaatg aactgccaca atttcgctcc
    50461 cgccaggaac aacatcgcca gcgtagtcac aaacaacacc accaccgtac caaggtctgg
    50521 ctgtgccagc agtaacactg ccaacaccag aatcacgccc atcggtttca ggaagccgcg
    50581 caggttatta cgtacttcgt cgcctttacg caccagatag ttggcgatat agcaaaacag
    50641 cgacagtttt gtcagctccg caggctggat acgcagcaaa ccgagatcga tccaacgcga
    50701 tgccccttta acagcgctac ccactaccag cacgatcatc agcaggatga tagatccgag
    50761 cagcatcgtg gcactgtagc gttgccagaa ctccatcggc agacgcagcg taatgatcgc
    50821 cagaataaac gccaaaatca gatagacacc atcacgcttc gcgaagaaga acggatcgtt
    50881 ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc atgataaagc caatcgccgc
    50941 gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt
    51001 ttcccgcgag cccatcaccc agccctttag cgccgtggag atccagacca ggatactgaa
    51061 tcctggcagg cgcggcattt tcaggcgagg gagagataaa cgcatcaacc taactccttc
    51121 gccagacggg caaactcatt gcctcgttgt tcaaagttct tgaactgatc aaggctggca
    51181 caggccgggg agagcagaac catatcgccc ggctgaacac gcggagccag caagcgcata
    51241 gcctgttcca tagtttcggt ttgttctgcc acttccgggc gtagcgccgc cagctgcgcg
    51301 ccgtcacgac cgaaacaata cagacgtacg ttatcgccat tcaggtaacg caccagtggg
    51361 ctaaagtccg ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc gtctacgtgc
    51421 aggccattca gcgccgcttc cgtactgccg acgttggtcg cttttgaatc gttaatccag
    51481 cgcacaccgt tatgctccag caccacttca aagcgatgcg gcagaccggt gaaggtggtt
    51541 aacgctttca ggctgctggc gcgcggtaac cctgcggcat ctgccagcgc cagcgccacc
    51601 agcgcattgg tgtagttatg ctgcccggaa agtttcatct ctttcacatt cagcactttc
    51661 tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat tcaggtgata gtcacccatg
    51721 ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt taaggcatca
    51781 tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc gcagttttgc tgcacgatac
    51841 tgttgtaaac caaacgggta gcgatccata tgatcttcag tcacgttcag aatggtcgct
    51901 gctaccgcct gtaagctgga ggtggtttcc agctggaagc tcgacagttc cagcacgtac
    51961 agttcacact catcatccag tagcatcaac gcaggcaggc caatattgcc acccacacca
    52021 acgttaaccc ccgccgcttt cgccatttca cccactagcg tggtgaccgt gcttttgccg
    52081 ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc gacaaaacag ctcgatatcg
    52141 ccaacgattt cgattccggc atcagcggca gcgcttaagg atggatgcgc cagtgcaata
    52201 ccgggactgg cgacaatcag atctgccgcc atcagccatt catcattcag actgcccgtg
    52261 tggcgttcta cggcttcggg taatttatcc aggccaggcg gtgtcatacg cgtatccata
    52321 acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc ggtgaggccc
    52381 aggccgataa tgacaacatt tttaccctgg taatcagcca tgattaacgt accttcagcg
    52441 ttgccagacc aatcaggacc agcatcagcg aaataatcca gaaacgcaca atgacgcgcg
    52501 gttccggcca gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg aaaatacgtt
    52561 gtccgcgcag tttaaaggag ccgacctgca ggatgacaga aagcgtttct accacgaaca
    52621 cgccccccat aatcaccagc aggaattcct gacgtagcag tacggcgata atgcctaacg
    52681 caccacctaa cgccagcgaa ccgacatcgc ccataaagac ctgcgccgga taggtgttaa
    52741 accacaggaa gcccagtcct gccccgacta tcgcggtaca gacaataacc agttccccgg
    52801 cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt catgttgccg gtcgcccacg
    52861 ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat aattgccaga ccgtcaagac
    52921 catcggtcag gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc agcagaatgt
    52981 agaacagccc cagttgcggc atcacatctt taaagaatgg gaccaccagc tgcgttgcgg
    53041 gcgtgtcttt gccggcaagg tacaaagcga aagcaacacc cagcgcaatg accgacatcc
    53101 agaaatattt ccaacgagcg atcaaccctt tggtgtcttt acgcaccact ttgcgataat
    53161 catcaacaaa gccaataaca ccgtaaccta ccagcaccac caacacgcac cagacgtacg
    53221 gattggacgg gtaagcccac agcagcacgg agatcacaat cgctgtcagg atcataatcc
    53281 cgcccatggt cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg ttacgcacta
    53341 cctgaccaaa ggaaagtttt tgcaaatgag caatcatgcg cgggcccatc cacaatgaga
    53401 tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa aagacgttaa
    53461 agccggaata atatttgacc aaatgttcgg ccagccaaac taacatgtcc cattctcctg
    53521 taaagcgcgt actacctctt ccatggcagc actacgtgaa cccttaacta aaatcgtaat
    53581 tacctgttgc tcagcaatca gtgatttaag acgcgtaatt aacgcagttt tatcagcaaa
    53641 atgttcgcca acgccgctgg cggtgctgat agcatggctt tgtttaccca tgcttaacac
    53701 gcggtcaata ccagcagctt ttgccgcctc acccacctgt acatggcagg cttcgctttc
    53761 agcgcccagt tccgccatat cgcccaccac cagcacgcgg tagcccggca tttcagccag
    53821 tacctgaaca gctgcggtca ttgaaccgac attggcgttg taggagtcgt cgagcagcaa
    53881 ctggttttct gccagttgga tggggaacag acggccagga acagctttca gatttgccag
    53941 ccccgctttg atagcatcaa gcgttgcgcc cacggacatg gagagcgcag cggctgccag
    54001 cgcattcgca atattgtgac gccccggcaa cggcagcaga acatcgacgc taccggttgg
    54061 ggtttgtagg gtaaattcca taccgtgcga ggtcacatgg atattggtgg cggtgaaatc
    54121 gctgttggcg gcattgggtg agaaacgcca cactttgcgt gagccaatta cgctctgcca
    54181 gttcagccag tcgttgttgt cggcgttcat aatggcgata ccgttttccg gcaggccgct
    54241 aaagatttca cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt ccagatgcgc
    54301 cgctgccagg ttgttgacca gcgccgcttc cgggcgagtc agactcacag tccaggcaat
    54361 ctcgccctga tggttcgcgc caagttcaat aactgcgtaa tcgtattccg gcgttaagcg
    54421 caacagcgtc atcggtacac cgatgtcgtt gttgagattg cctgccgtat aaagcgtgtt
    54481 gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc cggaggaccc
    54541 cgtcagagca accacgcgcg ccggaacttg ctggcgaacc catgcagcca gttcaccaaa
    54601 cgccagacgc gtatccttga cgattaactg cggcaggtcg atgtccagcg gacggctaac
    54661 cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca agatcgtggg catcaaaacg
    54721 ttcacctttc agggcaacaa acaggcagcc cggcgtcagt tttcgggtat cagtggttac
    54781 agcatcaagg gtgatatctg caccttgcag ttcaccgttg agaatatcgg taagttggct
    54841 aagggttacg ctaatcatgc aatccccccc agcagacgcg ccaccgtgac gcgatcggag
    54901 tagtccagac gctgattgcc aacaatctgg taatcctcat ggcctttgcc cgcgaccagt
    54961 accacatcat tctctttagc ctgcataacg gcgcaagtca ccgcttcagc acggccttcc
    55021 atcactttgg catgtccggc atctaacatt cccgccagaa tatcgttgat gatggcacgc
    55081 ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa ctcttcggca
    55141 attgcgccca tcagtggacg cttaccttta tcgcgatcgc caccacagcc aaagacgcac
    55201 cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag tgcatccggc
    55261 gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg cagtgaacac ttccatacgt
    55321 ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat cagccagtgg atagcccagc
    55381 gccagcagtg ttgccagcgc gagcaacagg ttgctgacgt taaaagcgcc catcagatgg
    55441 ctttcaattt cgccatcgcc ccagcttgaa ctaaagcgaa tggtcgcgcc gctgtcgtga
    55501 tagttcactt cggtcgcttt caaccagcgt ccgtgacagt tcggattgat atgatcttcc
    55561 attgataccg caaccgcgtc cggcagtttt gccagccagc ggcggcccac ttcatcgtcg
    55621 gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa gcagccattt cgcagcttcg
    55681 tagtgttcca tatcaccatg ataatcaagg tgatcgcggc ttaagttggt aaagaccgac
    55741 gccggaaatt tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga aacttccatt
    55801 gcgcaaaaag tcgcgccctg atccaccagc cccgccagct catgctgaac atcaactgcc
    55861 gaaccggtag tattttctgt cgggatcact ttccccagca gaccgttacc aacggtgccc
    55921 attaccgcgc tggtttcgcc aagcagttgg ctccactgcg ccagaagctg ggtcgtcgtg
    55981 gttttgccgt tggtgcccgt tacgcctacg agacgtaaat tgtcagaggg ttcatggtaa
    56041 aagcggcccg ccagtgcaga taaacgctcg ttgagctggc tgagatagat gaccggtacg
    56101 ccgtgcattt cacggatttc accgtcggtc gcctcatctt tcgcctctgc aataatggca
    56161 gccacacctt gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg atgacctact
    56221 acagctacaa agagatcgcc cgccgcagcc acacagctgt cgagtgtcat ctctcgcagt
    56281 gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt acgatctgcc
    56341 acctgtcccc tcgccttgat taatcacaaa ttcattttta tcgcccgttg tcagcgcatc
    56401 cggctcgatg ttcatggtac gcaatacgcc gcccatgatg gcaccaaaga ccggcgcgga
    56461 aacggcgccg ccgtagtatt tacccgcctg cggatcgttg ataacaacaa ccagcgcgaa
    56521 gcgcggctga ctcgcaggcg caacgcctgc ggtataagca atatatttat tgatgtagcg
    56581 accgtccggc ccgacctttt tcgcggtacc ggttttaatg gcgatacgat agcctttaat
    56641 cgccgccttc acgccgccgc cgcctggtag cgccacgctt tccatcatat gcaccacggt
    56701 gcgaacaatg gattccggga agacacgttc accgggaacc ggggggtcaa ctttggtaat
    56761 cgacagtggg cgataaatgc cgtagctgcc gatagttgcg tagactcgcg ctaactgtaa
    56821 cggtgttacc attagcccgt agccgaaaga gaaggtggcc ctctctatgt cagaccaccg
    56881 ttgtttttga ggatataagc cactgcgttc tccgaccaac cccaaattgg tcgcttttcc
    56941 cagtccaaaa cgtgagtaag tatctactaa cgctgaggac ggcatcgtta acgccagctt
    57001 ggaaacaccg acgttactcg acttctgtaa taccccggtc agggttaatt cgctgtagcg
    57061 tgccacgtct ttgatttcgt ggccgttaat tcgataagga atggtattga gtaccgagtt
    57121 ttcccgcacc acgccacgtt gcaacgcggt catcaccacc atcggtttaa ccgttgagcc
    57181 cggttcaaac acgtcggtga tggtacggtt acgcatcgac tctttcggcg tgccgctcag
    57241 attgttaggg ttgtatgacg ggctgttagc catcgccagc acttcacctg tgttgacatc
    57301 caccagcacg gcgctaccag attcagcctt gttaaaggcc accgcgttgt tcagttcgcg
    57361 ataaaccagc gcctgcaggc gttcatcaat actcagcgcc aggttgtgcg ctgcctggct
    57421 gtcagtagaa gaaatatctt caattacgcg accatagcgg tctttacgca caatgcgctc
    57481 acccggctgc ccggtaagcc atttatcgaa actcttctca acgccctcaa tcccttgact
    57541 atcgacgtta gtaaagccga tgaggtgagc agtcacttcg ccggacggat agtaacggcg
    57601 agactcttca cgcagatgaa tccccggcag tttcagtttt ttgatgtagt ccgccatgtc
    57661 agagtttacc tgacgcgcca gataaataaa gcgccctttc gggttggcgt taatgcgggc
    57721 agaaagctga tccagcggaa tattgagcgc gttagccagc gccttccagc ggtcaccgac
    57781 gctgataccg ccagcgtcat gcacttcttt cgggtcagcc caaatcgctt ttaccggcac
    57841 gctcaccgct aacgggcgac cagaacggtc agtaatcatg ccgcgggagg tggaaacttg
    57901 ctgaacgcga agagaacgca tgtcgccctc tttcaccagc atatccgggg agataacttg
    57961 taaccacgct acgcgtccga gcagaaaagc cagcgccagg agaatacagc cgcataacaa
    58021 cgcaaaacgc caactgataa agttggcatg ttcttcctga cgttttggtt tctgcgtttt
    58081 cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc actacgatat tttcttgtga
    58141 cggatcaaca tgctgcattt gcagcttttc cgtggcgatc ctttccaccc ggctatggtc
    58201 gccgagcgca ttctcttcaa ggatcaggtt gcgccattca atgtctaaag catctcgctc
    58261 cagcaccagt tgttcgcgct gagcggtcag taaacgggta tggtgcgccg tggttaccac
    58321 ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc caaatcgcaa
    58381 aagatcgtca ccgataacac caggcaatgc atggcgctcg tggcttccca tcgatccttt
    58441 aactttgctt agagcttctg tcactctgct gatcatgcgt tcgtcctctc tgcaatacgc
    58501 agaactgaac tacgggcacg agggttctca gccacctctt cttcgcccgg cattaacttg
    58561 cctagtgctc gcagctgacg gccacccagt tttttgagct gctcttcagt catcggtaac
    58621 cctgccggaa cttgcggacc gcggctgttt tcacgcataa aacgtttcac aatacggtct
    58681 tccagcgagt ggaagctgat gatcgaaagc cgcccacccg gggccagcac gttgagcgag
    58741 ctttttagcg cctgctctat ctcctccagt tcactgttta cccaaatgcg caccgcctgg
    58801 aaggtacggg tcgcgggatg tttaaactta tctttcaccg gcgttgcagc agccacgact
    58861 tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac aatggcgcgg
    58921 gcaatgcgtt tggcaaaatg ctcttcacca taggttttca atacccaggc gatatcggct
    58981 tcttctgcgg tttgtagcca ttcagcggct gactgcccac gggttgggtc catacgcatg
    59041 tccagcggac catcgcgcat aaaggaaaag ccgcgttcag catcatcaag ttgcggtgaa
    59101 gagacgccaa gatcgaggag aatgccgtcg atcttgccaa taagatcgcg ctcggcaacg
    59161 tattcgccca gcgcggagaa aggtccgtgg atgatggaga agcgcggatc atcaatagtc
    59221 ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc ctcttcgcca
    59281 agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc aatgtagatg
    59341 ccatcaggac ggatattgag gccattaacg gcttcatcca gcagcaccgt agtatgttta
    59401 tagttttcca tcattttata gagacaagtc ctgcagtcgc tccgataagt ctccggtagc
    59461 caactgctct gcgtcgatat cttccttgac ctgttgatgc caggttgttt catcccacag
    59521 ctcaaacttg ttgaactgtc caaccagcat cacttctttt gtcagcccgg catgttgccg
    59581 cagtactggc gcgattaaca atcgacctgc gccatccatc tgacattcgc tggcatgacc
    59641 taacagtagg cgctgcacac ggcgctcaac cgggttcatg ctcaacagac gcgataattt
    59701 ttgctcgata atttcccatt caggcagggg gtaaagcagc aggcacgggt gatgaatgtc
    59761 aatggtgcaa accatttgac cggcagcgtt ctcaagcagc tgttcccgat aacgggtagg
    59821 cactgataag cgccctttgc tgtcgagatt gactaacgtt gctccccgga acatgccagc
    59881 ctcacccctt attcccactt tgccccacaa tttcccactg aaaggagttt acggagcgga
    59941 ggaaaagctt gtcaagccag tcacaaggca taccgaggta aaaaacccga tgtttacggc
    60001 taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc aaattaacgt tatgaatagc
    60061 tgtaagaaaa aatccctcat cgtcatcact gttttaaaat gactcactat tttccacagc
    60121 aaatataaag tgtcagtttg cgacgcgagc ggcattttag gacatatctt ccccggttaa
    60181 cagtccttgt tacgtctgtg tggcgcttga tgccagcggg tttgcgcaaa agcaaagaaa
    60241 agtgtttgtt aaatcggcaa ttcatcgccg catgtaacaa aataatgcaa aaacgcgctg
    60301 tcgatatcac ttaattattg tgaatttcag ctggtgagtt aattaacggg cgggtttaaa
    60361 accatttatc aataatagtc aggaatagtc ttatttactt taaggatatt gat
//
LOCUS       DAEEGA010000013        59845 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEEGA010000013 DAEEGA010000000
VERSION     DAEEGA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59845
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.013"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="KLH46_000834"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="KLH46_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBE0377631.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="KLH46_000835"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="KLH46_000835"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBE0377632.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSLDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="KLH46_000836"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="KLH46_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBE0377633.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="KLH46_000837"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="KLH46_000837"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBE0377634.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="KLH46_000838"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="KLH46_000838"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBE0377635.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="KLH46_000839"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="KLH46_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBE0377636.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="KLH46_000840"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="KLH46_000840"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBE0377637.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYDEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATKWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="KLH46_000841"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="KLH46_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="KLH46_000842"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="KLH46_000842"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBE0377638.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPLLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="KLH46_000843"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="KLH46_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBE0377639.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="KLH46_000844"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="KLH46_000844"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBE0377640.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QTQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="KLH46_000845"
     CDS             complement(13538..13933)
                     /locus_tag="KLH46_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBE0377641.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="KLH46_000846"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="KLH46_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBE0377642.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="KLH46_000847"
     CDS             complement(15220..15447)
                     /locus_tag="KLH46_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBE0377643.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="KLH46_000848"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="KLH46_000848"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBE0377644.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="KLH46_000849"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="KLH46_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBE0377645.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="KLH46_000850"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="KLH46_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBE0377646.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18383..19480)
                     /gene="mltA"
                     /locus_tag="KLH46_000851"
     CDS             complement(18383..19480)
                     /gene="mltA"
                     /locus_tag="KLH46_000851"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBE0377647.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19689..19765
                     /locus_tag="KLH46_000852"
     tRNA            19689..19765
                     /locus_tag="KLH46_000852"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19723..19725,aa:Met,seq:cat)
     gene            19799..19875
                     /locus_tag="KLH46_000853"
     tRNA            19799..19875
                     /locus_tag="KLH46_000853"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19833..19835,aa:Met,seq:cat)
     gene            complement(19950..21203)
                     /gene="amiC"
                     /locus_tag="KLH46_000854"
     CDS             complement(19950..21203)
                     /gene="amiC"
                     /locus_tag="KLH46_000854"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBE0377648.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21435..22766
                     /gene="argA"
                     /locus_tag="KLH46_000855"
     CDS             21435..22766
                     /gene="argA"
                     /locus_tag="KLH46_000855"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBE0377649.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSLHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22828..24654)
                     /gene="recD"
                     /locus_tag="KLH46_000856"
     CDS             complement(22828..24654)
                     /gene="recD"
                     /locus_tag="KLH46_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBE0377650.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24654..28196)
                     /gene="recB"
                     /locus_tag="KLH46_000857"
     CDS             complement(24654..28196)
                     /gene="recB"
                     /locus_tag="KLH46_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBE0377651.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28189..31077)
                     /gene="ptrA"
                     /locus_tag="KLH46_000858"
     CDS             complement(28189..31077)
                     /gene="ptrA"
                     /locus_tag="KLH46_000858"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBE0377652.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31253..34621)
                     /gene="recC"
                     /locus_tag="KLH46_000859"
     CDS             complement(31253..34621)
                     /gene="recC"
                     /locus_tag="KLH46_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBE0377653.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34634..34966)
                     /locus_tag="KLH46_000860"
     CDS             complement(34634..34966)
                     /locus_tag="KLH46_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBE0377654.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(34951..35358)
                     /locus_tag="KLH46_000861"
     CDS             complement(34951..35358)
                     /locus_tag="KLH46_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBE0377655.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35355..35918)
                     /locus_tag="KLH46_000862"
     CDS             complement(35355..35918)
                     /locus_tag="KLH46_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBE0377656.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35909..36379)
                     /locus_tag="KLH46_000863"
                     /pseudo
     CDS             complement(35909..36379)
                     /locus_tag="KLH46_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36563..37357)
                     /gene="thyA"
                     /locus_tag="KLH46_000864"
     CDS             complement(36563..37357)
                     /gene="thyA"
                     /locus_tag="KLH46_000864"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBE0377657.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37364..38239)
                     /gene="lgt"
                     /locus_tag="KLH46_000865"
     CDS             complement(37364..38239)
                     /gene="lgt"
                     /locus_tag="KLH46_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBE0377658.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38390..40636)
                     /gene="ptsP"
                     /locus_tag="KLH46_000866"
     CDS             complement(38390..40636)
                     /gene="ptsP"
                     /locus_tag="KLH46_000866"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBE0377659.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGSANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40649..41179)
                     /gene="rppH"
                     /locus_tag="KLH46_000867"
     CDS             complement(40649..41179)
                     /gene="rppH"
                     /locus_tag="KLH46_000867"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBE0377660.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41864..42553
                     /gene="mutH"
                     /locus_tag="KLH46_000868"
     CDS             41864..42553
                     /gene="mutH"
                     /locus_tag="KLH46_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBE0377661.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42622..43335
                     /locus_tag="KLH46_000869"
     CDS             42622..43335
                     /locus_tag="KLH46_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBE0377662.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43473..43691
                     /gene="ygdR"
                     /locus_tag="KLH46_000870"
     CDS             43473..43691
                     /gene="ygdR"
                     /locus_tag="KLH46_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBE0377663.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43799..44839
                     /locus_tag="KLH46_000871"
     CDS             43799..44839
                     /locus_tag="KLH46_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBE0377664.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRCQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44872..46062)
                     /gene="lplT"
                     /locus_tag="KLH46_000872"
     CDS             complement(44872..46062)
                     /gene="lplT"
                     /locus_tag="KLH46_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBE0377665.1"
                     /translation="MSESVHINTSLWSKGMKAVIVAQFLSAFGDNALLFATLTLLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVVIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46055..48214)
                     /gene="aas"
                     /locus_tag="KLH46_000873"
     CDS             complement(46055..48214)
                     /gene="aas"
                     /locus_tag="KLH46_000873"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBE0377666.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48800..49831
                     /gene="galR"
                     /locus_tag="KLH46_000874"
     CDS             48800..49831
                     /gene="galR"
                     /locus_tag="KLH46_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBE0377667.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49838..51100)
                     /gene="lysA"
                     /locus_tag="KLH46_000875"
     CDS             complement(49838..51100)
                     /gene="lysA"
                     /locus_tag="KLH46_000875"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBE0377668.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51222..52157
                     /gene="lysR"
                     /locus_tag="KLH46_000876"
     CDS             51222..52157
                     /gene="lysR"
                     /locus_tag="KLH46_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBE0377669.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52144..52836)
                     /gene="ygeA"
                     /locus_tag="KLH46_000877"
     CDS             complement(52144..52836)
                     /gene="ygeA"
                     /locus_tag="KLH46_000877"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBE0377670.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52965..54383)
                     /gene="araE"
                     /locus_tag="KLH46_000878"
     CDS             complement(52965..54383)
                     /gene="araE"
                     /locus_tag="KLH46_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HBE0377671.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGGLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54698..55459)
                     /gene="kduD"
                     /locus_tag="KLH46_000879"
     CDS             complement(54698..55459)
                     /gene="kduD"
                     /locus_tag="KLH46_000879"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBE0377672.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTAQGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(55489..56325)
                     /gene="kduI"
                     /locus_tag="KLH46_000880"
     CDS             complement(55489..56325)
                     /gene="kduI"
                     /locus_tag="KLH46_000880"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBE0377673.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56612..57793)
                     /locus_tag="KLH46_000881"
     CDS             complement(56612..57793)
                     /locus_tag="KLH46_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBE0377674.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58048..59277
                     /locus_tag="KLH46_000882"
                     /pseudo
     CDS             58048..59277
                     /locus_tag="KLH46_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14775 a        16053 c        14575 g        14442 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 ctggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gataaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagcctgg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttccaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggca gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatgacgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccaaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgtcggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgctgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgagt
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat agcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcagggggc ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcaccctgtc atatccgtaa
    18361 aaacggcata cagaatatca catcagccgc taaagacgtt acctgcgccc ggagccgttt
    18421 tcagcaccca aacccgtcca tagtggttgt accaacctgc gcggtgtccg gcttccggcc
    18481 cgatcccttg atagatatcg aagtgttggc ctttgattgc gcccccgaca tccaacgcca
    18541 ccatcagacg caattcgtac tgaccgttaa atttgccgtt attatccagc agcggcactt
    18601 ctgccagcaa ggtagtacct ggcggaataa ttgaacgatc ggaggcaaca gaagcacggc
    18661 caaccagcgg caccgcactt gcccctttca ccggtgcaaa agattgcggt ttaaagaaga
    18721 cgaaagacgg gttctgttcc agcagctcgc gaacctcggc ttcactgtgt gtttcgcccc
    18781 aatgacgaat cgcctgcatc gacatatctt cttttttcac ttcgccacgg tcgatcagca
    18841 ccttaccaat gctgcgatag gcatgaccgt ttttccctgc atagctgaaa aagttaagcg
    18901 gactgccatc accaaagtcg atatacccac taccctgcac atccataatg aagttatcca
    18961 tcagagagtt gctgtaagcg aggatgtatt tatcgcccaa tgccccggcg tagatctccg
    19021 cacgagacgg cagacgacca cgttttggcg gcatacggta aataggatac tggaactcgc
    19081 cctggcgggt atggcgcgcc tgaattaccg gcgtgtaata gcctgtaaac tgcacgttac
    19141 catagttgtc ggcaccttcc atctgccagg catcaatgcc gaactggcgc atattgcggg
    19201 tatcaccgcc tgcgcgcagc cactcttgca ccgcgttata aacattactc tggttgccat
    19261 acagacgcgg tgacgaatta cggatatggt taatttgctc ggcaaaatca ccggcgttaa
    19321 tcggcgcgcc aacggcatct ggctggttca ccagagagaa aggctgggta aatttcccgt
    19381 ctttatattg ctgtccgcga tcggttggtt tggaagagca ggcggcaagc attgccacaa
    19441 ccgtgcccat aagaaggtac tttacccaac gtcctttcat tgttctcttc ttcaggttaa
    19501 aaaataaggc gcaacgaaga taacaaaccg ccgcgtttaa tgaaatgagc agcctctccc
    19561 tgacgcaaat tttgcacaaa aaataggctt tagtggtttg tttttgttca aaatcatgct
    19621 aaatccgtga tcggggtaaa aaaaaggttg catgaaaacg cgagcggagt atagtgcgca
    19681 tccacggacg cggggtggag cagcctggta gctcgtcggg ctcataaccc gaaggtcgtc
    19741 ggttcaaatc cggcccccgc aaccaatcaa atttgatgaa gtaaaagcag tacggtgacg
    19801 cggggtggag cagcttggta gctcgtcggg ctcataaccc gaaggtcgtc ggttcaaatc
    19861 cggcccccgc aaccaattat tgaacaccct aacgggtgtt tttttgtttc tgatctccca
    19921 taaaaaaagc gccattcagc gcctttttat catccccttc tcgccagcgt tgccccatcg
    19981 gcaaaatacg ctttaatccc cgcaagaata gactccgcaa cttcctgctg gaaagtcgcc
    20041 gttttcagtt tacgctcttc ctcaacgtta ctgataaacg ccgtttcgac cagaatggag
    20101 ggaatatctg gtgcctttag tacggcaaac ccggcctgtt caacttgatt tttatgcagc
    20161 ttgttgattt ttccgagctt attcagtacc gctttaccaa acttaaggct gtcagcaata
    20221 gtcagcgact gcaccatatc gaacatggtg tggtcgacat agcggtcacc gcttttgctc
    20281 acgccaccaa tcaagtccga ggcgttctgg gtttgtgcca gatattttgc cgcagtactg
    20341 gttgcgcctt tggttgagag cgcaaacacc gaggaaccgc tcggctgacg actggtaaag
    20401 gcgtcggcat ggatagagac aaacaggtca gcacgctgtt tctgggcttt tgctacgcgc
    20461 acttgcaacg gaatgaagat gtcttcattg cgcgtcatgt acaccttcat attgccctct
    20521 tcctcgatca gagagcgcaa acggcgagct atttgcaata ccacatcttt ttcgcgcgtt
    20581 ttgtatttcc ccaccgcacc ggagtcttcg ccaccgtggc cagggtcaag cataatgaca
    20641 atcggacgat cgcgccctgc tttacccggt tgtggaccac tttgcgctgg cggcacctgc
    20701 ttttcgaggt cgcctttgtt gtaatcctcc agcagcgcca gcagcgggtc ctgcatatcc
    20761 tgtgcattgg ccggatagag gtccatcacc agacgctctt taaacccggc gaccggcgca
    20821 agggcaaaca gctgcggttt tacgttttgc tttaattcaa aaaccatgcg tacggtttgc
    20881 gggtcaaatt gcccgacgcg cgccgacttg atgaacgggt cgtctgcacg aatttgtgcc
    20941 gccatcccct tgagtaccga gttcaggttt acatcttcga tatccaccac cacgcgttca
    21001 gggttactca acgcgaactg cttatatttc agctgacgat ttgattctac cgtcacgcgg
    21061 gtgtagctgg acgcaggcca gacgcgcacc gccacgacct ggctaaccgc agccaggctg
    21121 acctgactta cgctcaatag ccacatagcg cccgcgcctt gcagtaaacg acgacggctg
    21181 attgcagtgt tggatcctga catgcctctc ccgagcaaaa gaaatctaag ctgtgtaaca
    21241 agtaaacgac taatttgacc ggaaacttta gcgaaagacg cataatctgt catctaataa
    21301 acggtaaaca ttctttttat attcacggca ttactgataa aaaagtcgct ctcgcataaa
    21361 atttacactt gcaccctgcg aaaaaacaga ataaaaatac aataatttca aataatcatg
    21421 caaaggggtg tgccgtggta aaggaacgta aaaccgagct ggtcgaggga ttccgccatt
    21481 cggttcccta tatcaatacc caccggggaa aaacgtttgt catcatgctc ggcggtgaag
    21541 ccattgagca tgagaatttc tccagtatcg ttaatgatat cgggttgttg cacagcctcg
    21601 gcatccgtct ggtggtggtc tatggcgcac gtccgcagat cgacgcaaat ctggctgcgc
    21661 atcaccacga accgctgtat cacaagaata tacgtgtgac cgacgccaaa acactggaac
    21721 tggtgaagca ggctgcggga acattgcaac tggatattac tgctcgcctg tcaatgagtc
    21781 tcaataacac gccgctgcag ggcgcgcata tcaacgtcgt tagtggcaat tttattattg
    21841 cccagccgct gggcgtcgat gacggcgtgg attactgcca tagcgggcgt atccggcgga
    21901 ttgatgaaga cgcgctccat cgtcaactgg aaagcggtgc aatagtgcta atggggccgg
    21961 tcgctgtttc agtcactggc gagagcttta atctgacctc ggaagagatt gccactcaac
    22021 tagccatcaa actgaaagct gaaaagatga ttggtttttg ctcttcccag ggcgttacta
    22081 atgacgacgg tgatattgtc tccgaacttt tccctaacga agcgcaagca cgggtagaag
    22141 cccaggaaga gaaaggcgat tacaactccg gtacggtgcg ctttttgcgt ggcgcagtga
    22201 aagcctgccg cagcggcgtg cgccgctgtc atttaatcag ttatcaggaa gatggcgcgc
    22261 tgttgcaaga gttgttctca cgcgacggta tcggtacgca gattgtgatg gaaagcgccg
    22321 agcagattcg tcgcgcaaca atcaacgata ttggcggtat tctggagttg attcgcccgc
    22381 tggagcagca aggtattctg gtgcgccgtt ctcgcgagca gctggagatg gaaatcgaca
    22441 aattcaccat tattcagcgc gataacacga ctattgcctg cgccgcgctc tatccgttcc
    22501 cggaagagaa gattggggaa atggcctgtg tggcagttca cccggattac cgcagttcat
    22561 cacggggcga ggttctgctg gaacgcattg ccgctcaggc gaagcagagc ggcttaagca
    22621 aattgtttgt gctgaccacg cgcagtcttc actggttcca ggaacgtgga tttaccccgg
    22681 tggatattga tttactgccc gagagcaaaa agcagttgta caactaccag cgtaaatcca
    22741 aagtgttgat ggcggattta gggtaagcga tgaaaatcac cggatgcggc aggcgtaaca
    22801 ctcgtgcgtc gcatccggca attacgttta ttcccgcgaa ctaaacaacg ccgccagacc
    22861 actgcgccgc tcagtacgag tggcgattgc cgcacttaat atacgctcat cggcatacag
    22921 cgacagacga cgacgcgcgc gggtcaccgc ggtgtatacc agctctcgcg ttactaccgg
    22981 cgtgcgttgg ctcggtaaaa tcaacgccgc atggtcgaac tccgatccct gcgatttatg
    23041 taccgtcatc gcccacgtag tttcgtgctc tggcaagcga ctcggttgca cagacttaat
    23101 attgccgtcc ggcatcgcaa accagacgcg cgtcccctgc ccgcgatcca gcgcaatacc
    23161 gatatcgcca ttaaacaacc caagcgcgct gtcattacgg gcaatcatca ccggtcggcc
    23221 ttcgtaccaa cgagagtgcg gatgacgatg aattttgcgc ttctgttgca taaactgttc
    23281 aattcgctca ttcagtccag ccacgccaaa cggcccttcc cgcagggcgc acaaaagctg
    23341 gtactcattg aacgcctgaa tgattaaatc cggctcggca cgcgcttgca gcagatccag
    23401 ataacgtccg taacccgcaa gagcttcctc aagcatcgca atataatctt cgccgctctg
    23461 taaaagtcgt ttttcgatat cagtaaaatc ctgctgaaaa acggttttca ctgccgtttt
    23521 atcaccacgg ttgatcgccg cagctaactg accaatgcca gaatcgctgc cgaaacgata
    23581 gcttttttgc agcaggcaga gactgtcgcg caaagatgcc gcttctgtgc cggttcctgc
    23641 cggaacgtga gtccccgtca ggcggcttag ctgcctggca cgctcggcgg taaagcccgc
    23701 gttggcataa gcgcagatat cgcccagcac agccccagcc tcaaccgagg ccagttgatc
    23761 acgatcgccg agaaagatca ctcgcgcatg atctggcaag gcgtcgatca gtctcgacat
    23821 cataggcaga tcgatcattg acgcttcatc taccaccagc acatcaagat gcagcgggtt
    23881 accggcatga tgacgtaaac gctggctacc cggctgcgcg cccagcaatc ggtgcaaagt
    23941 gctggcatct tccggaatgc gtttcttttg ttcatcggtc agcggtaact gtcgcaaagc
    24001 cttgccgaga gattcggtta agcgcgcggc agctttaccc gttggtgcag ccagacggat
    24061 acggcagcgt tcgccgtcgg ccatttgaat taacgctgcc agcaacttcg ccacggtggt
    24121 cgttttaccg gtgcctgggc cgccggaaat caccgagatc cgccgcgtca gcgccactgc
    24181 cgctgcaact ttttgccagt taatttcatc gcttactgga aaaagtttgt ccagggtttg
    24241 cgccagtaga gcttcatcaa cctcaatggt atgattcacc tcattgaaga agcgcgctac
    24301 cgtccgctcg ttacaccaca tgcgattcaa ataaagacga tcgccacaga ggatcatcgg
    24361 cgtgggttca tctcccctgc tgaccgcttg agaagccagc aagcattctt cccaattttg
    24421 tagctcaccg atttcactga cacaggtcgc caacagcgga ttcgacgcct cgttattttc
    24481 cagtcgtgaa agcggcaaac agacgtgtcc ctctccggca tcatgactta acagtgccgc
    24541 cgcgagggtg acggcaggat gttcatctcc cgccacggtc agggcaaatt gcacatccag
    24601 cgggcgtagc tgtttgtgct ccacagcttc cagtaattgc ttttgcaatt tcattacgcc
    24661 tcctccaggg tcataccggc aaacatctca tccatcaggg caatcaaccc ggcgttgggt
    24721 cgggttgtgt aaatcccttg ttgcggatgt tctttatcaa cgccacgcag gaacagatag
    24781 ataacgccgc caaagtgaag atcatagtcg taatcagcaa tgcgatggcg cagataacga
    24841 tgcagcgcca gggtataaag ctgatattgc agatcatagc ggtgtgcctg catcgctgct
    24901 gccatagcct gttgggtgta agccgaactg tcttcaccca gccagttgga tttatagtcg
    24961 agcaggtaat aacgcccttc gtggcggaat accaggtcga taaagccttt taacatgccg
    25021 cgtacctgca tgaactccag cggcgggcag ccagcggata gcgggtcaaa ctggcgaatt
    25081 agtgcatcaa gctggctggc gatgagcggt tcgctaatcg gcaggtaaaa ctccatctcc
    25141 acctgtttat cgcggtcgga aagctgactc aggctaacac ccgtttcatt gagaggtgcc
    25201 tggaggacag ccgtgatcca ctcggtcaac accggttccc actgcgattc aaagccgccg
    25261 agttccagtt tttcctgtac ccagttcggg tcaaccggct gggtaaaatc gaggtcttca
    25321 aacaaactgt gcaagaatgt ccccggtgac gcaccgcgcg ggaactgatg cggtgttaac
    25381 gtcggttctt caacgacgct gaccacgcct gcggcatcaa catccagccg tggcatcaga
    25441 tcctgagcga taccgtgacc acgctgctgc aagccggagt agctggtgac acgccagtta
    25501 tcgccgggca atcgttgtag tgtcctcgca ttcagttctg cagttaaagc atcattaacc
    25561 tgccagggct ggttatcacc agtttgtgcc gtttgccagg caatatcatc atcgcataac
    25621 gcttcaatac aggtgcgaag ccctgccgca tcttgcggtt cccctttttg cagcaaacgc
    25681 ccgagcgcac tttggtggac gtcggtgtca ccttttttat cgccacgacg gcgcaccagc
    25741 ggtgcaacgc cgagactgca atgccaaacc gaacgcgtca gcgccacgta aagcaaacgc
    25801 agatcttccg ccagacgttc gacctccgcg aggtcgacgc tttctggcgc agcattaaga
    25861 tccaaaactg cctcaaacga gtggcgatcg tgataaaacg cctgatcctg gacgcggaaa
    25921 ttggtaataa atggcaacca gaccagcgga tattccagcc ctttcgattt atggatcgtg
    25981 acaatctgca ccagatgttt atcactttcg aggcgcattt gttggctgga ggcattactg
    26041 tctggctcga ggatatgttg cgataaccag cgtaccagcg catgttcact ttccagctgc
    26101 gttccggctt cttgaagcag ttcgctgata tgcaagatat cggtaagacg ccgctcaccg
    26161 cctgccgttg ccagcaagtt ttctgcaatg ttacgcgccg acatcagcgc ccgcagcatc
    26221 ggcataacgc cacgtttgcg ccagatttgc cgataaccat cgaactcttc gactaccgca
    26281 tcccacgcat gttcgtcatt attcagcgtt tcaatatcca gcgcattcag ccccatcatt
    26341 gacgttgcca gcgcactgcg cagggtgttc tcacgttcgg gcgtcatcac cgcctgcaac
    26401 aaccaaagca tttcctgcgc ttccagagtt tcaaaaacac tgtcgcggtt cgaaaggtaa
    26461 acggaaggga tttccagcaa cgttaaggca tcgcgcacct gggcggcctc ctggcggctg
    26521 cgcaccagca cgctgatgtc cgaagcacgc accggacgcg cgtcgtcgcc gttcatcagc
    26581 aacgcttcgc cccgctgtcc ggcttgtagc cagtcgcgga tttgcgcagc acatacctgc
    26641 gccatggtac tttgatagtc gccaacgccg cagctttcgc cttccatcag ccacattttc
    26701 atcgcaggct gcgtttcacc tttaaataca aaacgtaacg cctgattttt cccggctgat
    26761 ttcactggaa taaacggtat ttcgcgaaac atgaacgtgt catcagtctg gctgaaaagc
    26821 ttgttcacac tgttcaccat tccaggtgcg gagcgccagt tggtgtctaa agtgtagtgg
    26881 gcgtgaactt cactacgcgc cttcatataa gtgaagatat ccgcaccccg gaatgcatat
    26941 atggcctgct tcgggtcgcc aatcagcaac aatgcggttt ccggctgatg gtgccagata
    27001 cggcgaaaaa ttcggtactg ctgggggtcg gtatcctgaa attcatcgat cattgccacc
    27061 gggaatcgcg tacggatcgc cgctgccaac acctcgccgc tttcgctacg cagcgcggaa
    27121 tcgagccgac ttaacatgtc atcaaaaccc aattcgccac ggcggcgttt ttcacgcgct
    27181 actgtttcgc ggatctcagc caatgcgcgg gtgatcaaca gatcgcggat cgacaatggt
    27241 tctgcaagca gttgatcgat cgcctcaaac agtggatgtc gcggggtttc ccccccggcc
    27301 ttcgtgcgat cttctaagaa acgctgggag aatttttcca gcgactccgg caactgataa
    27361 ctgtttgtct cttcttctgc ccaggcgctg atcttttcga tccatttagc ctgattgcta
    27421 cggttaaact tgcgtcgatc aataccagaa gattcgatca gcgcatccag ttcacccact
    27481 gcgtcgcgcc actgctgttt taccgtatca atacgcgcca caatttgcgc gtggcgggaa
    27541 gccagcgttt catcatcggg cggcggtgct ttgataaccg gcggttcgcc ttgcagataa
    27601 cgattaatat cgcgcagcaa cgcctgcggc cctttccagg tttcaaagac gacctgggct
    27661 atttcacgcg gcagcgggta gcagtggcga cgccagaaat cggcgcaggc ctggtagcgt
    27721 agcagagact catcttcaat cagctgctgc tcaaacagca tgccggattc aaaggcattc
    27781 aggttgagca tgcgctggca aaagccgtga atagtaaaga ctgccgcttc atccatctgc
    27841 cgttcggcta acaacaacca ctgcgcggct tgcgctttat cgtcgatctc ttccagcagg
    27901 cgttcgtaca gtggattgtc ggtggtttca cgcagacagg cgatgcgcaa ctcgtggata
    27961 ttgctacgga tacgaccgcg caattctgcc gtggcagcct cggtaaaggt gaccaccagc
    28021 agttcttcaa cgatcagcgg gcggggaaag gcggcggaac cgcctagtcc aagtaacagg
    28081 cgcaaataga gcgccgcaat cgtaaaggtt ttgcctgtgc cggcagaggc ttcaatcagg
    28141 cgctcgccct gtaagggcaa gcgcaaagga tctagtgtct cggcgacatc actcattctt
    28201 ttcactcatc aggggcattg tttgctgcaa cgcgctgacg ttctcccaca ctttccagcc
    28261 ttcaggatgc acatattcgg ctttcccgtt ctggctgccg gaaatctgcg acagaatagc
    28321 catgccttgc ggctcgacca ccgcctgatg gaagaaatca gcaagttttt gcggcgtcag
    28381 cagttttatc tgggccacga ttttatcacg cgaatcgaag cgcatattgc cgcgatcgaa
    28441 atctttactt aacttcgatg cttcttcgcc gagcgtttgc ggtgcctgca gcatctgggt
    28501 aattaccgcc tgctggattt gcgcaaactc atctggcttc atcgctcgca attttgcctc
    28561 tgcggttggg aaaaacgcct tgtaacgctc ccacaagaat gaaggctgtt tatcattgct
    28621 ttgcaaaagg aagcccatgc cccactgacg ccccacgctc attggaaacg caaacacggc
    28681 atagcccaat tgttcttcgg tacgcaactg attgtagaac cacggctgta cgatctgccc
    28741 caacagagag ctataggctg agctggtgta ttcatcgtag ccagtcggta caaataccgc
    28801 tgccagtgcg gagtcggtgc tgttaccggc tttttcaaag atgacggatt gttttttatc
    28861 gaccactaca tctttgtttc gacaccactc tgaaccatca gcgcccaact gtttttgcac
    28921 atcgcgtgcc agcgttgttg cctgggcctc ggtcatgttg ccgataacca taaactctgg
    28981 tcgagcccct gattttaagg cgtcgcgata ggccagcacc tctttcaacg taatggaggg
    29041 caaaatttta cgccgttcat ctcgcgagaa gtacggcact tgcgagagca tctgcgcggg
    29101 cataatcgcc tgctcaaacg ctttaccctt ttctgcggaa tccatcatct ggttatacca
    29161 ggacttcgcc tgctcaagct gctcttccgt agcggtatag ctaaagtacc cctcgagcaa
    29221 tgcctggaac agctgcggca gacgctgggt gtaaccatta gcattaatca taaggccgtt
    29281 gttagcgttg gtggaaaaac ttatgccacc aaccgacgcc tggttgctta actgatcaag
    29341 cgccagccct gcgagataat cattgagcgc aaacatcacc tgattgcggg cgctgtccat
    29401 ggctttcgga ttacgcaaaa tcaggctgac atcagctttg ggctcgctgg caaaataacg
    29461 gcttggcgca tacaccacgc gcagattcga ctcatcaaca atcagctctg gatggtcgta
    29521 tttcttctct gacttaatca gcgagaaatc atcaggaata taagggttaa gctctggcaa
    29581 agagagcgca atgtcggcgg cttttttctg ccagtcggcg aaagtttgtg cgctgatttt
    29641 atcgacctga tacggcgcat cgacaaagta agccgttttg ttgtgcggct ctttcgggct
    29701 gatataccag atacgcgcat tctgcggcgt catcatcgcc agacgttcct ttactgcttt
    29761 agcatcgtac cgatcggcaa tattgactgc atccagcgta tgctcaacag gaacgcgaat
    29821 catggtatct gccagccatt cgacgtaatc catatcacgg gtgatcgacg gataacggaa
    29881 gtcgatatcc agcacattcg ccagttcatc gaagtattgt ttatcaatgc ctttttcacg
    29941 taacagattg agatagctaa aaattgccgc cacaacctga tcgcgattag ccaagccttt
    30001 atcagttaaa gacgcagaga tcgctaatac gccgctgttg ccgttgacga taggatcgga
    30061 gttggcgcta atgccctcaa ctaatccctg cttttgcagc cagtcagaaa gtgtacctgg
    30121 gctgcgattg ccaatcagat aggtaatcaa ttcatcggtt ttactacgga acttcgctga
    30181 gttgttatcg atgcgaaact caacgcgcaa cactttacgc ggcagcgcag ggacgtaatg
    30241 aatgataatg cccttttgcg cgtcggtgac taccggcacg gtgatttccg gttttttgct
    30301 ctctttgttc ggcacgcgac caaaggtgtc cgccgccatt tttgccaact ccggcagcgg
    30361 tttattactg taaataaccg ccttcatcaa attggcggaa tagtacttct cgtggaaatc
    30421 tttcagcgcc tgctgcaccg gattaccagg tttgtcgctt aaagtttcga ggttaccacc
    30481 agaaaacttt gaaccggggt gtgccgggtt aatggtttct gcgctgacct gtgccatgcg
    30541 catcccgtca cgcgtacgcg ccatggttaa ttcagcgttc actgcattac gctcacgttc
    30601 ggcgtatttc ttgtcgagca agggttctgc aatagcatcg gccaggcggt ctaccgcacc
    30661 gggcaaggcg tcgttctcaa cttccagata gaaagccgtg cgatacggcg cagtgctggc
    30721 attgtgacta ccgccgtgca ttttgagata ttcggccaga ctgtcagcct gcgggtactt
    30781 tttcgacccc atcagactca tatgttcaag gtaatgtgcc agcccctggt acgcctcggg
    30841 atcttccagc gacccaacgg gcactaccag cgccgagagc gatttaactg cctgcggatc
    30901 agaaaccagc aagaccacca taccgttatc cagacgtata gcctgatact ggcggttatc
    30961 tttatcactt ttacggatgg tttcctgaat cggctgccat cccgtttctg cctgacttaa
    31021 tggtgcccag agggcaacaa acaacaataa tgctttgaac caggtgctgc ggggcattca
    31081 cggacctcat aatcaacttt attttctgga cagattcaac aggttaacgc aaatcatctg
    31141 ccagaattta atcttgtgct gcacgagtca gcctatgttt atataaccat cagtccgtga
    31201 ctggtgcgca tcataaagta agcggataga ttgcgcaatt tttatacaac actcatgact
    31261 gattaaagcg aaacagcggt aacaggaaac gttgcgactg ttcaacgatg gcctccattg
    31321 tctctggtgt taattgccgc cagagccgtt gataccagat atcatcacct tcgccacgca
    31381 ccatcatgtt gccttcgtaa gcctgaagga atttcgtacg ggctttttgc aacgtggaat
    31441 cgtcatccag catggcatcg ttttgcgcgt cataacaggt ttttagccac gcgccgccac
    31501 tttcaggtaa caccagcaat ggcgcggaca ttccttcacg atacccctca atcagttgtg
    31561 agaggtaatg caaagcctgt tcggctgcaa gcggcggaaa acgccactca ccgtctttgc
    31621 gtagaaaaag gcgactttcg ccattaccac cgctggcaca gtagacaagg tgttccagcc
    31681 aaagttgcat tccctgcgcc acacttaata aagagggacg ccagcgcaac aggccatccg
    31741 gctgcacctg cggcaaccag ccagttatct gcacaccgtt gcaggcgaga tcaatttcca
    31801 tactctgccc cggctggcga caggcaatga ctctgtcggc aagctgctgc atctcctggc
    31861 actgtgtttc ccagaaaatt tcaccaaaag cgccatacgg taaatcccct gccgcccgga
    31921 agcggcggaa caagcgttcg gcatcatcct gctcaaccag tgcattcaat aactgctgat
    31981 tgatttgata acggctaagt ccttccagaa taaatggctc ggtgtctggg atttcgctgt
    32041 cttcagtatg aaagttcacc tgcaaacgca tctggaagaa cgcccgcacc ggatgtgccc
    32101 agaatcgttg tagcgtttcc agcggcacgg tttccggtaa ggtaaacggc agcggctgaa
    32161 caaattcaga atgtgcttta ccagcctggc tggccgcagg tagccattca cgagcatagc
    32221 tttgtcgttc gcctggctgg tagttttgcg gatcaaacgg catccgggta tggaggcaag
    32281 taagatgcgc ttttaccctt gcctcgcttt catcacagtt gagcgcttca tcgcccggta
    32341 gataatgact ttgcccgatg tagtcgatca gttcctgcac cagtaccgac gggaaacgtt
    32401 cactgttatc ctgaatggaa cgcccgatat agctgatata gagtttttgc tgcgcggaaa
    32461 ttaacgcttc caggaacagg tagcggtcgt catcgcgacg gctacggtcg ccacgcttcg
    32521 gtttctggct catcaggtca aagcccaatg gcgcaagctg acgtggataa acgccgtcgt
    32581 tcattcccag caggcaaacc actttgaacg gaattgaacg cattggcatc agagtacaaa
    32641 tgttaaccgg tccggcgaga aaacgctggc tgatacgttc ttgatccaga cgctgtgcca
    32701 gttcatcacg caatagtgac agcggcaccg cgtcgccata ctgcgcacct aaaccttcgg
    32761 cgataatcgc ctgccattgt tgttcgatca gcgtcatcgc cgcttcggtt tccgcatccg
    32821 gcaggaagaa ggcgttgagc atatcgcgac aaaccggcaa ccactcttct agcggacgct
    32881 cctgtgccag cccgcgacgc cagatgttta gctgcattag cagtgaagcc agatgcccca
    32941 ccagttctgc aattaagccg ctcgattcat cataaggtag aaccgattgc cactcgccct
    33001 gcgcgctctc catcgcgtag cccagcaaca tacgcgtcag gccaaatcgc caggtgtgtt
    33061 gtccggtggc ggggagttcc agctcgcgaa cgttgtcgtc atctatcccc caacgaatgc
    33121 cggattcgtt gacccactgg cgtaaataac gcagcccttc ttcggtgatg tcaaaccgcg
    33181 ccgccagcac cggcacatcc agcagcgcca gcacatcctc tgacacaaag cgactgtcag
    33241 gcagtgataa caggctgata aacgcttcca gtaccggatg tgactgccgc gcacgacggt
    33301 cggaaatggc gtaaggcagg taacgatccg caggtgcgct accaaacact gcctgaataa
    33361 acggactgta gctgtcgata tcagccacca tcacgatgat gtcgcgcgga gtaagtgtcg
    33421 gggcttcctc cagcatcgcc agcaagcgat cgtgtaaaac ttcaacttca cgctgcgggc
    33481 tatggcaaac gtggaatgtg atactgctat ccagtggatc aagcgggcgt ttgttatcgc
    33541 tacgggaaaa ctcttcgatg ttcacacccg caacggcgcg gttttccagt tccagaatgt
    33601 cagactgaat gttatgcagc aggttatctg gcgtcacatc gacaaaagcg tccagctcct
    33661 ggctgctctc caggtcagaa aggagataaa tgtagtcgcg cccaagcttc ccccatgagg
    33721 ccagcagcga gttgccgaca tcctgttcac catcgctgtt aaagagctgt ccggcatttt
    33781 cgctatcgcg aaatagcggc aattcgcgat cttcaaaact gtgtcgacgc tgacgagtca
    33841 gcagtttcgc cagataagca ggatctttaa tatcgcccca gtaataacgg caggggttgg
    33901 taaacaggag atggatttca atatgtttac ccagcgcctg gagcgcctgg agataaacag
    33961 gcggtaacgc ggaaataccg catataaaga cgcgcgaagg taaccccggc gggcaggtcg
    34021 tcgcggactc cagcgtttcg ataaagcgct gatagagatt cgcgcggtgc cagcgcggtt
    34081 gtccgagttc atgggtatat tccaccagcg ccttccacaa cggggcttgc caggcctgtg
    34141 cttctcccag cccttcaacc aaatgtcctg tttcccactg tgccagccag tccggacgat
    34201 agaccagata ctggtcaaac aggtccgccg cttttgagga aagctggaac agttttcgct
    34261 tgtcgctatc gtcagtcaga taatgccgca acagggtaaa gtcttcgcgc tccagcaatt
    34321 gcggcagcag agtcatcagt ttccagctca tgctctgttt gttaaaggcg ctctctttgg
    34381 ggatttccgg taacacccgg acgaacatat cccagataaa gctcgctggc agcggaaaat
    34441 caatgtttgc cgcaatacca aacttttgcg acagggtcat ttgcagccac tgcgccatac
    34501 cggtactttg caccagaatc atctctggtt cgaaaggatc gtccagccgt tcgcgttcga
    34561 caataaactc catcaacgct tccagcacgt ccagacgatt ggaatggtag acccttaaca
    34621 tagcggctcc tgactactga cgattcgggc aatgcaggcg ggtcatctcg ccttctctgc
    34681 ccccgggtga aactagcgta acgctgatgc tgacacatcc cgcctgcgat gtctgcattc
    34741 ggttgacctg ccagttggca ggtggcgaaa tcgcgcgcag ttgcgtttgc tgccaaccat
    34801 gccgccagag ctgttggtac tggtttctgc tggcgagact gttcattgat gttcgctgga
    34861 tacccgataa cgcagtgaca atcatcacca tcagcaccat cagcaccatc gcaaccatta
    34921 cctccggcag gctaaagcct tgttgattct tcagggaagc tgacataatg ccccctcttt
    34981 cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca atttttccat caatgacttc
    35041 gcctgttcgc cacaacgaaa cgccttcata accggcaatc aataaggctt cattatctgc
    35101 cagtaaacgc aaacaaacct gggcatcggt tccagcgtac tgcgagcact gaactgctgg
    35161 ctgcgtctgc caggagtgca tttttcccca cgccagcgcc gactgaacga tggcctggcg
    35221 gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct gctgactcat
    35281 tccttgcaat agcaagctac ccaaaaccag cagcatcagg accagagcca gtgacgaaac
    35341 acctttttcg cggttcacag gttgaatcct gtcacgctat agctggcatc caccacggtt
    35401 tgcggttcag acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg
    35461 acatcctgac gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta
    35521 tcccagccct taccttcaca ggatgtcgca ccgcgtagcg tttccagcac atgctccttc
    35581 agacgaaatc caatctggtc ggactctttt accggttcgc gatcccagat accgttactg
    35641 ttcgcatccc actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg
    35701 gtacagctgc catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc
    35761 agccagattt catcttccag tgccagctta cgagtgctcg ttaaactttc acgctgtaac
    35821 gcaggcagaa agcgtgcagc ccccagcaac aatacgctac tgatcgccat agcaatcaac
    35881 acttccagca gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgat
    35941 aaagccggag tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga
    36001 agcgaatatg cccggcccat gcggtattac gcaggccaaa gaaagcaagc gaaggtgtca
    36061 ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac
    36121 aggtattggc cccagcagcg gaactcacaa ggcaccataa cgtcccctcc ctgataacgc
    36181 tgatactgtg gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc
    36241 gcgcctagct ggcggtttgc caaagccgtt gcgactgctg ccagtattgc cagccataga
    36301 gtccacttgc gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat
    36361 aaccacgttg tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag
    36421 tttctatttc ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt
    36481 tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa accgacgcac actggcgtcg
    36541 gctctggcag gatgtttcgt aattagatag ccaccggcgc tttaacgccc ggatgtggat
    36601 cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt
    36661 tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca
    36721 tatggttgct gtacaggtgc gtatcgccac cggtccagac aaaatcaccc acttccagat
    36781 cgcactgctg cgccatcata tgcaccagta acgcgtagct ggcaatgttg aacggcaggc
    36841 cgaggaagac gtcacaggag cgctgataaa gctggcaaga gagtttgccg tctgccacat
    36901 agaactggaa gaatgcatgg cacggtgcca gcgccatttt atccagttcg cctacgttcc
    36961 acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt cagctggttc agtaccgtag
    37021 taatctggtc aatatgacga ccatctggcg ttggccaggc gcgccactgt ttaccataca
    37081 ctggcccgag gtcgccattt tcatcggccc attcgtccca gatggtgaca ttgttttcgt
    37141 gtagataagc aatgttagta tcgccctgca ggaaccacag cagttcgtgg atgatggaac
    37201 gcaggtggca acgtttggtt gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct
    37261 gatgaccaaa aatggaaagc gttccggttc cggtacggtc gtttttctgt gtgccttcgt
    37321 cgagcacttt ttgcatcagt tctaaatact gtttcatggt tcctcaggaa acgtgttgct
    37381 gtgggctgcg acgatatgcc cagaccatca tgatcacacc cgcgacaatc atcgggatgg
    37441 aaagaatttg ccccatgctg atgtactgca cccaggcacc ggtaaactgc gcgtcgggct
    37501 ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac aagcctgaga
    37561 cagctcccat tgggcgcggt ttacgaatat acaggttgag gataataaac agcaccacac
    37621 cttccagcag cagttcgtaa agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga
    37681 aaatggattg ccactgcggg ttggtttgca gcagcaaaat atcttctgta cgggagcctg
    37741 ggaacagcat ggcaaacggg aagttcgggt caacgcggcc ccacaattca ccgttaataa
    37801 agttgcccag acgcccggca ccaagaccaa acggaatgag tggtgcgata aaatcagaga
    37861 cctggaagaa ggaacgttta gtacggcggg cgaagataat catcaccacg ataacgccaa
    37921 tcaggccgcc gtggaaagac atgccgccgt cccagacacg gaacagatac agcggatcgg
    37981 ccataaactg cgggaaattg tagaacagaa cataaccaat acgtcccccg aggaagacgc
    38041 cgaggaagcc cgcatagagt aagttttcaa cttcattttt ggtccagcca ctgcccggac
    38101 gattcgcccg acgtgttgcc agccacattg caaaaatgaa acccaccaga tacatcaggc
    38161 cgtaccagtg aagcgccacg ggtcctattg agaaaatgac cggatcaaac tccggaaaat
    38221 gcagatagct actggtcatc tgtcaccaca agttcttgtt atttcgctga aagagaacag
    38281 cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat agcacaaggg
    38341 gacctggctg gttgccggat accgttaaaa gatatgtata tgatccgcgc tataaccctc
    38401 cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc tgcaacctga tgacgaactt
    38461 cggtcgccag ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa
    38521 tgcgccgcag caggtatttt acccgcgcta cagaacgtcc gttcatagac agatggcgat
    38581 acccaagccc aatgaggatt gccacgcaca tgggatcgcc cgccatttca ccgcacaaac
    38641 gaagatcgat accatgtatt tccgcttccc gggcgatcat cgccagagct cgtaacattg
    38701 caggatgaag actgtcgtaa atgttcgcca cccgggtatt gttgcgatca acggccagaa
    38761 tgtattgagt cagatcgttg gtgccaacag agatgaaatc gacccgcttt gccagatgcg
    38821 gcagcataaa taccattgac ggcacttcca gcatgatgcc gatacgtggt ttgggaattt
    38881 cgtaaccgat catctcctcg acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt
    38941 caacttcatc gaggctggtg accatcggca acagaatatt caggttgccc gtagcggcat
    39001 tagcacgcag catcgcccgc acctggatca agaagatctc cggctgatcg agcgtaatgc
    39061 gaatcccacg ccaacccagg catggattct cttcgctgat cggcatgtaa ggcagctgct
    39121 tatctgctcc gacatccagc gtacgcaagg tgacgggttt atcattgaac atttgcagca
    39181 tcccctgata ctgcgccacc tgttcttctt ccgacgggaa accactttgc agcatgaatg
    39241 ggatttcagt gcgataaaga ccgatgccat caatacagct gcccagtttt tcttcatgtt
    39301 ccgggcttaa accagcattg agcatgactt ttatacgctc accgcttttt aactgcgcgg
    39361 gtaaattgac gtcatcttcc gccagacggc taagctcaat ctcttcacta attagccgct
    39421 gatattcttg cagcagtacc ggctccggat cgaccagcaa ttcaccgcga tagccatcaa
    39481 cgatcagcgt cctacgatgc agcaccgaag gctgaatatc cgcgcccatc acggtaggga
    39541 tccccagcgc acgtaccatg atcgcagcat gggagttggc tgagccatct cgcacgacaa
    39601 caccgactaa gcgatcctgg ggcagctcag caagagttgt cgctgacagt tcatctgcca
    39661 ccagaatgaa acgttccggc caggcgttcg gcccttgatt agcgtcatca agatgaaaca
    39721 gcaatcgctg acccagcgca cgtaaatcgc cagcccgctc tttgagatag ttatcgctta
    39781 gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt taccgcccac tctgccaccg
    39841 agcctttatc aacctcggca aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg
    39901 agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt
    39961 agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg cgttccagag
    40021 ccggatccag cgttgatgcc tgatacacct gttccattaa aggtaacgtg gcatcctgcc
    40081 agccttcggc aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat
    40141 attgcccaaa cagggcagtc aactgcgact gagaaagaat agctgccatc tgggtggcaa
    40201 gcgtcaccag gaaggattct tcactttcgt catactggcg caactctcgt tgctgtacca
    40261 ccagtacacc aagcaactgg cgacgttgaa taattggtac gcctaaaaac gcgcggaaac
    40321 gttcttcttt tacggagggg atgtatttga agctggggtg cttttgcgca tctgcaaggt
    40381 ttatcggttc cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta
    40441 cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat caggtagtaa caacgtcgat
    40501 catgatcggc caggtagacc gaacagacct cggtatccat cgcaagacag atgtcggtaa
    40561 ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc taccttttcg actatttcgc
    40621 gcaggcgagt gagcataatt ggcgtgactt aacctctttt acgtcgataa gcagatgcgt
    40681 tttgtggttt tggcgtattt tcctgcagtg acatcaccac actcgcgaac tctttcatta
    40741 ccctacggta gacatcacgt ttaaatgaca ccacctgtct gaccggatac cagtaactta
    40801 cccatcgcca gccgtcaaac tctggtgtac tgctggtttg catattgatt tctgcatcgc
    40861 cgcttaccag ctgcaagaga aaccattttt gtttttggcc gatacaaacc ggcttcgtgt
    40921 cccaacgcac caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga
    40981 ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa ttcacggtac atcgcctgct
    41041 ctgcggattc tccggggttg attccgcctt gcggaaattg ccaggagtgc tgaccaaatc
    41101 gccgggccca cattacctgc ccctggcgat tacaaatcac gatacctacg tttgggcggt
    41161 agccatcgtc atcaatcacc ggactacctc aaaataaagc tttatatacg aatgattgtt
    41221 tcatactcca ggaagacggt aaaccactct ctgcagggca ttacacacta ataacaattg
    41281 aataactcac agttatgtgc agagttataa acagaggaag gggtggatag ccgtttttat
    41341 tcaccttttc tgtggataga gttgtgaaga actacggaat tactacggga aaacccggag
    41401 cattctgaat aagcccgata tagaaatttt aaaatattca ttattagtca atgcattaca
    41461 tgtaatttga gtaatgaaaa agagtaattc gtgacccagg tcacacctct catttacggg
    41521 ttggcgaaag atcaaccaat gccgtattta tccacagaat gtgccactaa gttaagcact
    41581 gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg taaatgaaaa cagtagtgga
    41641 ggtttttcac agttatccca gctttctgtg gataacatgg tgtaagatcc tgtttatttt
    41701 cagtgaccag atttggaaaa cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa
    41761 aaagcaatat aaatcagaga attgaacaac acatgtggaa aaagttacac tgcgaatatt
    41821 cggcacataa ttactgtttg ttttttaacc aaggtatcat gacatgtccc aacctcgccc
    41881 actgctctct cctcccgaaa ctgaagaaca gttgttagcg caagcacagc aactttctgg
    41941 ttatacattg ggagaactgg cggcacttgc cgggctggtt acgccagaga atttaaaacg
    42001 cgataagggc tggattggcg tgttactgga gatctggcta ggtgccagcg cagggagtaa
    42061 acctgagcaa gattttgctg ctctgggcgt ggaacttaaa actatccctg tggatagtct
    42121 tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg ttaacgggta atagcggggt
    42181 gacctgggaa accagccatg tgcgccacaa gctcaaacgc gtactgtgga taccggttga
    42241 aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc
    42301 gaatgaagag gaagaccgac agctacgcga agactgggaa gaattaatgg atatgattgt
    42361 tctcggtcag gttgagcgga tcaccgctcg tcacggggaa tatttacaga taagaccgaa
    42421 agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc cggggcgaac ggattctgac
    42481 gctgccacgc ggcttttatt tgaagaagaa tttcaccagt gcactactgg cccgtcattt
    42541 tctgatccag tagccatcgc tttgacctgc cgctttccgg gcatataatt accgcttcat
    42601 ttttttggca gggcttttta gatgttattt gcatggataa ccgatcctaa cgcttggctt
    42661 gcgctcggta cgctgacgct gctggagatc gttcttggga tcgacaatat tattttcctt
    42721 tctctggtgg tggcaaagct tcccacagca caacgtgctc atgcgcgccg tctggggttg
    42781 gcgggagcca tggttatgcg tctggcgctg ctggcatcaa tctcttgggt tacgcgcctg
    42841 acaaatccgc tttttacaat attcagtcag gaaatttctg cccgtgattt gattctgctt
    42901 ctgggtggct tgttccttat ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt
    42961 gaagaagaag ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt gcagattatg
    43021 ctgctggata ttatcttcag cctcgactcg gtgattaccg ctgtgggtct gtcagatcac
    43081 ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc
    43141 tcgattggtg attttgtcga acgccatcct tcggtaaaaa tgctggcgct ctctttcctg
    43201 attctggtgg gctttaccct gattctggaa agtttcgaca tccacgtacc gaaaggttac
    43261 atctacttcg cgatgttctt ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa
    43321 aagaatccgc tctgatactc cgtacgctct cctgcgggag ggcgtactca ctccttttac
    43381 cctcacagtt taagattttc ctgctttcaa gatatataac gtcggtttat aaacagacta
    43441 ttatcatagg taggccaaat gaggataacc ggatgaaaaa atgggcagta ataatttctg
    43501 cagtcggact ggcgtttgct gtttccgggt gttccagtga ttacgtcatg gcgaccaaag
    43561 atggccgtat gattttgacc gatggaaaac ctgaaattga tgatgatacc gggctggtga
    43621 gttatcacga tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta
    43681 ttgaacgtta acaaataagg tcagcatccg gctggcctta agatttttct ctttcccttt
    43741 tcccttccct ctgccatttt tatattcctt atgtcgtgat tataaaaagg aaacggctat
    43801 gcaatatcac cgtatacccc acagttcgct ggaagtcagc acgctggggc ttggcacgat
    43861 gacgtttggt gaacagaaca gcgaagccga cgcccacgca caactcgact atgccgtcgc
    43921 tcagggcatt aaccttatcg acgttgccga aatgtaccca gtacctccgc gccccgaaac
    43981 gcaagggtta accgaaacct acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa
    44041 gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc
    44101 ggatcaggcg ctggatcgga agaatatccg cgaagcgctg catgacagcc tcaagcgcct
    44161 acagactgat tacctcgatc tttatcaggt gcactggccg cagcgcccga ccaactgctt
    44221 cggcaaactc ggttatagct ggacggattc tgcgcctgcg gtttcgctgc tggatacgct
    44281 ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga
    44341 aactgcattt ggcgtaatgc gctacctgca tctggcggac aaacacgatc tgccgcgtat
    44401 tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt tttgaagtag gtctggcaga
    44461 agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt tcggcacgct
    44521 gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg
    44581 gttcacccgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc
    44641 cagacgtcat ggccttgatc ctgctcagat ggcgctcgcg tttgtacgct gtcaaccgtt
    44701 tgttgccagc actctgctgg gcgcaaccac gatggatcag ctgaaaacta acatcgaaag
    44761 tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtac atcaggttta
    44821 tacttatcct gcaccataaa gattaacgcc ggttaaaacc ggcgttaaat attaatgacg
    44881 gcgctgccag atccacagcg ccgttattgc cagcgcaaat agcgcaccga agccaatgcc
    44941 aatgggcacg accgggatgc ctaccattac cgccagcgag taaatgccca gcatcaacaa
    45001 catggcgctg ttttcgccaa ggttttgtac tgcaatcgca ttccccgccc cgacgctttt
    45061 tttaccccgc tcctgtagca acgcattgag cggaacgaca aaaaagcccc ccagcacgcc
    45121 aatcagcatc aacaaggcat aggctggcag cagctcgtgt tgcagggaga aaatcggtac
    45181 caccacgcca atcaaaatcc cggctggcat acagcgtgac acggtttcca gcgtaaccag
    45241 cttcgctgcc gcacctgcgc caaccacgat accaatcact accatcgcgt tgagataggt
    45301 cggcgtagcg ttatcggtaa tgcccagcgc caccggtacc cacagcacca acaggaaacg
    45361 cagcgtgaca cccgctcccc agaataaact ggtgcccacc agcgaaaaac gcgtttcacc
    45421 attgcaccat agcgaggtgc aggcattcag gaaactgcgg gtcatgttga tgagattcca
    45481 ggactgcccc ggacgcgccg ccagtttggg aatgtagata ttggcaacga ccgcaccacc
    45541 gtaggccagt gcgcatgcgg ccagagcgac caggacatgc cagtcagcca gcacaccacc
    45601 ggctacggaa ccgagtaaaa tcgccgctat ggcagaagct tccattaaac cgttagcttt
    45661 tactaactta ctacccgtgg ttaattcgcc gagaataccg tatttcgccg gtgaataggc
    45721 tgcagcacca acacccacca gcgtatagcc gagaaacgga ttgataccaa agcagatact
    45781 ggctgcgccc agcagcttca ggccgttggc aaacatcatc acccggcctt tggcgaagct
    45841 atccgccacc tgcccgacaa acggcgccaa aagaatgtaa gcacctacaa acaccatttg
    45901 caggatgggc tggctccact ccggatagaa ctgcgctttc agtaacgtca gagtggcaaa
    45961 cagtagcgcg ttatcgccaa acgcagagag aaactgcgcc acgataaccg ctttcatccc
    46021 cttcgaccac aacgaagtgt taatgtgcac tgactcactc atcgtgttgt tccgcttcgt
    46081 ctacccagct tttcaacgtg acaaagtcag gtttgccgct gccaagtaat ggcatctgtt
    46141 tcagatagcg aatatcgcgc ggtacagcaa gctccggcac gccgtgctcg cgggcatact
    46201 gttgcaactt atcgcgcgtc agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt
    46261 tgctggcatc gctcttaatc gcagtggcat ggactttatc tggcgaaaca ccaagtgcca
    46321 gttgttccac catttccagc gacaccattt cgcctgcaat tttggcaaag cgttttgcgc
    46381 ggccctgaat ctgcacaaag ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc
    46441 agccgcgctc catttcgccg cgaacattct cggcggtggg cacttccagt acacctggct
    46501 tctccacccg cagatagccg ttcattatgt tcggcccttt cagttgcagg cgtccgccct
    46561 cttcgatacc agggaccgac aacaggcgtg catccattcc tggtagaata cgccctaccg
    46621 taccgggttt cgccgccatc ggtacgttga tagaaacgac aggcgcgcat tcggtcacac
    46681 cgtagccttc aaggatgcgc aggccaaatt tatcctgcca aagctgtttg gtactttctt
    46741 gtagtttttc tgcgcctgcc accacatagc gtagacgata gaagtcatac gggttggcga
    46801 aacgcgcgta gtgaccgagg aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat
    46861 acaccaagtc cggcacaatg cggtaatgca gcgggcttgg ataaaggaac acttctgcac
    46921 ctgtaagcag tggcgtaaac aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg
    46981 ccgacataaa gcgatcgttg gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg
    47041 ccagaatgct tttatggcta tggacgacgc cttttggatg gccttcagaa ccggaggtaa
    47101 agaggatcag cgcctcttct tccggctgct gttttacctg cgccaggcgc ggcatcagca
    47161 aatgagcgaa gatccatact ttgtcggcag tggtgacatc tgcttttaaa tcttccagat
    47221 agacccagcg cacctgagta agttgctccg gcagatgcca gagtttgcct ttatcgagaa
    47281 actggcggga agtgaagatg gttttgattt cagccgccgt aatagcactg gtcagccctt
    47341 ttaccccggc agtgtagttc atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa
    47401 tcactgccgc actgatgcct gcattgggca gcattaagcc gatgcgttcg ccttcaacac
    47461 tgtatttttc aaggatgcgt ccaacaaaca gcgttttcgt aagcaatttg cgataggagt
    47521 ctggggtaaa gttgacgtct tcgacacatt tcttcccggc tccgaagcgg tacattgcac
    47581 tcagtaaaga ttcgtacaga gtttcacgcg ggcgcaccgc catacgcgct tccatcatta
    47641 tttgatgcag catttcgcca gcgattttgc gacggtcacg ggcacgcggc gcatccggca
    47701 tcgccacctg cgttggaggc aaaatatgca gagtaatttg cgggaacaag cgacgtttaa
    47761 ccagaccttt caggcggctg aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa
    47821 ccgttgcacc agacttcgcc gcgacaaaac ccgcgccatc gtagattttc atcagcgagc
    47881 ctgtagtggt gatgcgtcct tcagggaaaa tcaccactgg tcggccctgt tccaccagac
    47941 gtaccagatg tttaatagcc ataggttgcg tcgggtcgag aggaacaaag tcgataaatg
    48001 atttcagcca acgcatatac cactgttggc ttattgaggt gtaaacggca aacactggac
    48061 gcacaggtaa aaacagtcca agcaaaatgc catcaataaa agagacgtga ttaggcgtaa
    48121 ttagtacgcg ctcgcccttc agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa
    48181 cacggcacaa atttcgaaaa aagctaaaaa gcattccaac tccctttgct ctgattcagt
    48241 aaaagcgaat ggagggagat tacacgagat aaagaacgcg agcgacagta aattaggtgc
    48301 gaaaaaaaac ctgcgcatcc gcgcaggttg gtgcaagaga cagggtacga agagcgtacc
    48361 gaataatctc accaatcaat acctctggga tcttgattgt ggtctgcacg acgactcttc
    48421 gccagcgaga aaacgcaaag gaatgaaggg aaatgcaacg aggtgtgtaa attgtcggtt
    48481 actgttacag attgatgacc ggcaaaaaaa aacctgcgca tctgcgcagg ctggtgtaat
    48541 tcatgtgctc aacacgaagt tgacttcacc tatcaatacc tctgggatca ccactttagc
    48601 aacctgaagc caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa gtgtaaccaa
    48661 aggtttgcaa tctctttttt tgtcttactt atttgcgttt ggctcacatt cccacgatga
    48721 aaacacgcca ccccttgaac caacgggcgt tttccgtaac actgaaacaa tgtaagcgtt
    48781 tacccactaa ggtattttca tggcgaccat aaaggatgta gcccgactgg caggcgtttc
    48841 agtcgccacc gtttcccgcg tcattaataa ttcacccaaa gccagcgaag cttcccggct
    48901 ggctgtgcat agtgcaatgg agtctcttag ctatcacccg aacgccaacg cccgtgcgct
    48961 ggcgcagcag accactgaaa cggtcggtct gatcgttggt gatgtttccg atccgttttt
    49021 cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt tcttattgat
    49081 tggcaacggt taccacaacg aacaaaaaga gcgtcaggct attgagcaac tgatccgcca
    49141 tcgctgtgct gcgttggtcg tccatgccaa aatgatcccg gatgccgatt tagcctcatt
    49201 aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg
    49261 ttgtattgct cttgacgatc gttacggtgc ctggctggca acgcgtcatt taattcagca
    49321 aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg ccgaagatcg
    49381 tctgcaaggg tattacgatg cccttgctga aagtggtatt ccggccaatg atcggctggt
    49441 gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc ttttgggacg
    49501 aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg gcgcgatggg
    49561 cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg gctttgatga
    49621 tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc caatcgtaac
    49681 gatggcgacc caggctgccg aactggcttt ggcactggcg gataatcgcc ctctcccgga
    49741 aattactaat gtatttagtc cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct
    49801 ggaggcaagt catcatgcaa ccagcgacta accgcagtta aagcaattcc agcgccagta
    49861 attcttcgat ggtctggcga cggcgaatca accgcgcctg accattatca aacagaactt
    49921 ctggtaacag cggacggcta ttgtagttgg atgacattga tgcgccatat gcccctgtat
    49981 catgcagtac cagataatca cctgccttca cttccggcaa ggcgcgggtt tcaacatttc
    50041 ccccttcctg ctgggtaaag acatcgcccg attcacataa cggcccggcg acgacggttt
    50101 ccaccgttgg cgcgtgttcc agagaacgac catcagctgc cagggcactg atatggtggt
    50161 aactaccgta cattgccggg cgcatcagat cgttgaaccc ggcatcaacc agcacaaagt
    50221 ggcggctacc catttgtttg acgctccgca cctgagtaat taatacgcca gactgcgcta
    50281 ccaggaagcg acccggttca atttccagtt tcacagggtg gcccaaatgg cgagcgattt
    50341 gctcacgcgc ggcattccac agaccataat aatgttcggt atcaaccgcc tcttcaccct
    50401 gttgataagg aatagaaagc ccaccgcccg cagaaatagc ctgtaaatcc tgaccgaatt
    50461 cgataacctg acgcaccata gcaccacaca cctgctccag atgggcataa tcaacgccag
    50521 aaccaatgtg catgtgaatg ccgactagct gcagatgatg acgttgtatc acgtccagtg
    50581 cggcgggcag atcggtgtac cagataccgt gcttgctgtt ttcgccaccg gtattggttt
    50641 tttggctatg tccgtgacca aaccccggat taacgcgcag ccatacccga tgccctggcg
    50701 aaacctggcc cagttggtcg agcgtatcaa cagaacccgc attcaccgga atttgcaatt
    50761 cactgacgcg ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca atatcatcgg
    50821 ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc tatttcgcct aacgagacgg
    50881 aatccacttt cacgccctgc tcacgcatta agcgcaaaat atgaatattg gaacaggctt
    50941 tctgtgcaaa gcgcaccact tcaaactgtt tcagcgctgc aatctgccga cgaataattt
    51001 gcgcatcgta gacccacacc gggcagccaa attcagcggg caaacgcagc agattttcgg
    51061 cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc agatgagtgc
    51121 ttttttatga ttacgccaca tcataaaaag aataaaaaat atcgatttat gtcgagtcta
    51181 tgcaaaaatg atatggatta ccggattgcg agagagcgct aatggccgcc gttaacttac
    51241 gtcatattga aatttttcat gcggtaatga ccgccggaag cctgactgag gcagcacacc
    51301 tgctacacac ctcacagcca accgtcagcc gcgaactggc gcgctttgag aaggtgatcg
    51361 ggctgaaatt gtttgagcgc gtacgtggac gattacatcc taccgtgcaa ggactgcgtc
    51421 tgtttgaaga agtgcaacga tcctggtacg gactggatcg cattgtcagc gccgcagaaa
    51481 gtctgcgcga gtttcgccag ggagaactgt ctattgcctg cctgccggtc ttttcgcaat
    51541 cttttttacc gcagctcctg caaccctttc tggcacgtta tcccgatgtc agcttaaata
    51601 tcgtgcccca ggaatcaccg ctacttgaag agtggctctc ggcccagcgt catgatttag
    51661 gactcactga gacactccat acgcctacgg gaacagaacg taccgaatta ctctctttag
    51721 atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta ttaacgccgg
    51781 atgattttca gggtgagaat tacatcagcc tttcccgtac tgacagctat cgccagttgc
    51841 tggatcagct atttactgaa catcaggtta aacgacgcat gatcgtagaa acccacagcg
    51901 ccgcgtcagt ctgcgcaatg gtacgggcgg gggtaggtat ttcggtggtt aacccgctca
    51961 ccgcactaga ttatgcggca agcggtttag tggtgcggcg gttcagtatt gcggttccgt
    52021 tcaccgtcag cctgatccgc cccctgcacc gcccgtcatc agcgctggtt caggcgttta
    52081 gtgggcattt acaagcgggg ttaccgaaac tggtcacttc tcttgacgct attttgtcgt
    52141 cagctacgac agcataaaag cgacagcatc ctcggcatgg atcgccgcgg tatcaaacac
    52201 aggcaaaaca ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc caaaaatgac
    52261 gccctgtgcg ccctgttctg caaggcgagc aatcacttgc gcataataag cgcgtgacgc
    52321 ttcggtaaat tgccccagac acagttcttc aaaaataatc tgattaattt tcgcccgttc
    52381 atccgcttca ggaataagac aattgatgga aaattgttcc gtcagccgcc cgcgataaaa
    52441 atcctgttcc atggtgtaac gcgtacccag cagcgccaca cgagtcattc cggccccggt
    52501 aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac gtgactcaat
    52561 ggcatccgcc actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg
    52621 ctgtaagcca agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg
    52681 ctggcactct tctatttcat gaaaatcgac gctatgtagc agcatttgcg cagagtgaag
    52741 cccaccaagc cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc
    52801 ccagctcatt cctcccagca aaccaattgt tttcattcct ctctcctgat gtgtgttacc
    52861 gcaacaaggc tgaataagga acgagacaaa cgcctcaacg gccaggtgcc caatctctat
    52921 taacgaaaaa agggccggat gtacagcaca tccggcccgt gaaatcagac gccgatattt
    52981 ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac atttttggtt
    53041 tccggaatga gccagaaagt aatacccaca aacgcaatgt tcagcgcagt gtagagccag
    53101 aacgtaccgg cagcgccaat gctatcaagc agtgtcagga aggccgcgcc gataatcata
    53161 ttcgacaccc agtttgtcgt cgtcgaacag gtaataccga aatcgcggca tttcagcggc
    53221 tgaatttcag agcacaggat ccacaccact ggcgcggcgc tcatcgcata accggcaata
    53281 cacatcatcg tcatgccaac agagagccag gacaagccac tggaagccgt accgttatca
    53341 aactgcatca ggcaatagcc cagcaccaga gtgcctaacg ccatcacgct gaaaccaatt
    53401 ttcagagccg gtttacgacc tgctttatct accgtaaaga ccgcaatgaa ggtcgcgaac
    53461 ataaaggtca gtcccacgac cagagtcgca atcatctgtt gttctgtggt cgtaaagccc
    53521 gccattttga agatacgcgg cgcgtagtac atgatgatgt tcataccggt aaactgctgc
    53581 atcgcctgca acaacatacc gagaaacaca gcacgacgga cgttacggtt gatcttaaac
    53641 agtgcccaac cgccctgttt taacttcagg ctttcacgaa tttcgttgag ttcttctcgc
    53701 gctttttccg acgtatcgcg cagcatacgc aacacttctt ccgcctcaat atgacgcccc
    53761 ttttccgcca gccagcgcgg gctatttggc aggaagacca ccagaataat cagcagaact
    53821 gctggtaaag caagaccccc caacattgcg cgccagttac cgctataact gaacgctgta
    53881 tcggataaaa acgccagcac gatgccgagt gtgaccatca actggtacat actgatcatc
    53941 ttaccgcgaa cgttttcact tgccatttca gaaagataca gaggagcggt gtaagacgcg
    54001 atcccgacag caatgcccag caccacacga gcggcgatta acatctctac gctggtcgca
    54061 aaagcggacc ctatagaacc gagtacaaac aggatggccc ccgccatcag gctgtattta
    54121 cgccccaggc ggaacgacag ccaaccatta aacagcgcac caattgctgc gccgagcatc
    54181 atgctactaa ccacccattc ctgcaaacga ctggtcagca caaagtgatc ggtaatgaac
    54241 ggcaacgctc cggcgattac gccgatatca agaccaaata acaatcctgc gaccgcagca
    54301 gctaccgaaa caaacatatt catacgccgc gtatcacgca aagaacgtgg cgttaaagca
    54361 gattccgtat tgatagtaac cattttttcc tgccagcaga gagtaagaca ttgtgaaaaa
    54421 atacgtgaac aattcacaca ggtgtcaggt cggaaacagc ataaatatgg attaaattgc
    54481 tgcgacatgc cgttatgtga tggatattcc aattttaaaa ttaagttgaa ttattgagat
    54541 tattattaac ctcctaattt taaagcagat aaaattcata aagttcatta attgataatt
    54601 gatatggatt atttcataac catgatatgg attatgatga tctagagata taaaaaaccc
    54661 tgccatgcgg cagggtcata aaagtaagag gaatgaatta acgcgccagc caaccgccat
    54721 ccacagcaat ggcataacca tttacataat ctgaagcgct ggaggcaaga aacactaccg
    54781 gccccatcag gtcactcggc agtccccaac ggccagctgg aatgcgatcg agaatttccg
    54841 cgctacgttg ttcatctgcc cgcagttgtt gggtattgtt ggtcgccatg taacccggag
    54901 ctatcgcatt aacattaatg ttgtgtttag cccattcgtt tgccatcaat cgcgtcacac
    54961 ccatcacgcc gctttttgat gcggtataag aaggcacacg gatcccgccc tggaaggaga
    55021 gcattgacgc gatattgata atcttgccgc cattgccttg cgcgataaag tgtttcgccg
    55081 ctgcctgaga catgaagaat acgctcttga tattcaggtt catgacatcg tcccagtctt
    55141 tttcgctgaa ctcgagagcg tcttcgcggc gaatcaatcc ggcgttattc accaggatat
    55201 caatatgacc aaattcagct accgcgcgat ccagcagtcc aggaatgcca tcaatctttc
    55261 gcagatcggc ggtcaggctt aaaaaacgac gcccctgcgc cgtgacctgc tcgatggttt
    55321 cagtcggttc aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc agccccaacg
    55381 ccatcccctg gcccagccca gtatcacaac cagtgacgac cgcaacttta ccttggagag
    55441 aaaatgcact taaaatcata acaacacctt attcgtttat gcccacaact agcgcaaatc
    55501 tttaacgccc acatggtcca tatcattaaa gacctggttt tcaccgacca tgccccagat
    55561 aaaggtataa gctttggttc cgacgccgga atggatcgac cagctcgggg agatcaccgc
    55621 ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggc tgccccatca tgtggaaaac
    55681 gcaggcgtca tcatccatat tgaaatagaa ataaacttcc atccggcgct cgtgggtgtg
    55741 acacggcatg gtgttccaca agttacctgg agccagctcc gtcagcccca tactcaattg
    55801 acaggtttcc agtacatccg ggacaaaata tttgttaatc gtgcgacggt tactggtgag
    55861 gttatcgcct aacgtgactg gagatacttc gtccggtgtg acttttttgg tgggatacgt
    55921 cgtatgcgcg ggtgcgcaat tgtaataaaa cttcgccgga gtggcggtat caatactggc
    55981 aaagacaact tcttttgcac ctttaccaac atacagggcg tcgcggtgac cgatttcata
    56041 gcattgacca tcgacagtaa tcgtaccggc accgccaata ttgataacac ctaactcgcg
    56101 acgttcaagg aaatagctta cgccgagttg tttaccaact tccccgccaa cggaaaccgt
    56161 tttagttatc ggcataatgc cgccaacaat aattcggtca atgtggctgt aaaccatggt
    56221 gtactcatcg gcgacaaata ccttttcaac caaaaattca ttgcgcagcc cttgggtatc
    56281 cagcgttttt gcgtgcgcac cgtggatgct ctgtcttacg tccacatcaa cctccgataa
    56341 tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg tttcgttttc tctataaagg
    56401 gatgatatgc ccatgggcta gggttttcaa ttaaaaataa aacaatgttt tatttttaat
    56461 ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt catgagaatg gaaaaatgag
    56521 ggagcccaga aaatgtatgt cgacttgcca gccatcatta ttcaacagat taatgacggc
    56581 agatatttat cggtaaggag gaaatgcaga gctactcgtc acgttcaatg gtcaatgcca
    56641 cgccctggcc cccgccgata caaagcgttg ccagcccttt gcgggcatta cgtttcacca
    56701 tttcatgaac cagagaaacc aggattcggc aaccggaagc gcctatcggg tgaccgagtg
    56761 cgatcgcgcc accattgaca ttgacccgac gctcatccca ctcaagcatc ttgccaaccg
    56821 aaagcgcctg tgcagcaaac gcttcattag cctcgataag atcgacatca gccaactgcc
    56881 agcctacacg ctccaggcaa cggcgggtcg catacaccgg cgcaattccc atcaatgccg
    56941 gatctacacc aacgctggca aatgcgcgga tgcgggccag cacgggtaaa ttcaacgctc
    57001 gtgctttggc ttcgctcatc atcattaccg cagctgcgcc atcgtttatg gatgatgcat
    57061 tccccgcggt cacagaaccg agactatcaa atgaaggatt taaacgggct aaaccttctg
    57121 cactggcgtc agtgcgtggc tgttcatcgg tatcaacaac caacgtctga ccgttacttt
    57181 ggatcattac cgggacgatc tcatctttaa atcgtccggc gtcaatcgcc gctcgcgctt
    57241 tttgttgcga gctaagtgcg taagcatcct gcaactgacg gctgatgcca tattcgcgag
    57301 ccagattttc ggcggtgaca ccaatatgat aatcattgaa ggcatcccac aacccatcat
    57361 gcaccagact gtcaaccaac tggctattgc caagctgtgc accggtgcgg ctatcagtca
    57421 gaacatgtgg tgcgcggctc atgttttcct ggccaccggc gatgacaata tcagcctcgc
    57481 cacactgtat cgcctgagta gccagatgca gtgctttaag accggaaccg caaacgtcat
    57541 taatcgtgat tgcagaaacg ctgttaggaa gaccaccttt aatagccgat tgccttgccg
    57601 gattctgccc tgcccctgca gtcaacacct gaccaagaat tacttcatcc accgcatatg
    57661 caggaacgcc ggtacgttct attaacgctt tcacgaccag actaccaagt tccacggcgg
    57721 aatgacccgc taacgcacca cgaaagcagc cgataggtgt ccgtaacgcc ccgacaatca
    57781 caacgtcttt catcacaacc tcgcaccaat taacagtgca atagtaaatg attgttaatt
    57841 acagttatct taattgttta aaaaaagtga tttttatcac aaaggaaata tgcctgagca
    57901 gtagtcagag tcataactgg cacgtaaggt ttgcaaccac taacccacca atagatgggt
    57961 agatagggcg ttaatctccc atacttaact tggtttatgg taaattgccc tccattttgt
    58021 ttaatttgta gatgatacgt tcagataatg tctaatattt ggtctaaaga agaaactctg
    58081 tgaagtttcg cgctctatgg cacagccgtt gatgcaggca cgctattcct tcctattcag
    58141 ttaggttcgg caggggctgt ggtcctgttt attactgctc tggtcgcctg gcctttaaca
    58201 tattggccac ataaagcctt atgccagttc atcctctcat caaaaacatc agcaggtgaa
    58261 gggataacgg gcgcggtaac acactactat ggcaagaaga ttggtaatct gattaccacg
    58321 ctgtacttca tcgccttttt tgtcgtcgtg ttgatatatg cagtggcaat taccaactca
    58381 cttacggaac agctggcaaa gcatatggtt attgatcttc gcatccgtat gttggtgagt
    58441 ctgggtgttg tattaattct gaatctcatt tttctgatgg gacgtcatgc cactattcgg
    58501 gtaatgggat ttttggtatt cccattgatt gcctatttct tatttctttc catttacctt
    58561 gtcggtagtt ggcaacctga tctattaaca acccaggtag agttcaatca gaataccctt
    58621 caccagatat ggatatcgat tcccgtgatg gttttcgcct ttagccatac gcccattatt
    58681 tctacgtttg ccatagacag acgtgaaaaa tatggcgaac acgctatgga taaatgcaaa
    58741 aaaattatga aagtcgctta tctcatcatc tgcataagtg tactgttctt tgtctttagc
    58801 tgcctgcttt ctattccatc ttcgtatatt gaagcggcta aagaagaagg ggtcaccatt
    58861 ttatcggcgc tttctatgct gccgaacgcc ccagcatggt tgtcaatttc cgggattatt
    58921 gtcgcagtag ttgcgatgtc gaaatcattc ctgggtacgt actttggcgt tattgaaggt
    58981 gccacagagg tcgtcaaaac aacactacag caggttggtg taaagaaaag tcgtgcattt
    59041 aaccacgcac tatcaattat gttggtatcg ctgattacct tcattgtttg ttgcattaac
    59101 ccgaacgcga tttcgatgat ttacgcgatc agcggcccgc tcattgccat gatacttttc
    59161 atcatgccta cgctgtcaac gtatctcatc ccggcgctta aaccctggcg ttccatcgga
    59221 aatctgatta cgctgatcgt gggtatcctg tgcgtatcgg taatgttctt tagctaatat
    59281 ccgttgtgga tggttcaaaa ttgctacaat tttgaaccat tccatttttc ataatattca
    59341 tttgttatta ttccgcccta tcacttctct cgtcatatcc tagaattatc agttagttaa
    59401 acacacaaag agatctgaac atcttaaata agactattta agatgcataa cttagattcg
    59461 caagatatct tctggtcatt caaaaacaat ttgcgatata gatattctca taccatgaga
    59521 tagttatctg gaccacgttc gaccatgcaa gattaaatca gtgaaacata atcatattta
    59581 ataacgagaa atgcattttt aaatatctta atcagaatta tagcaaatac agattaatcg
    59641 caatttttcc cagcaaaatt acaaaatcaa ggtgtattga catcatcaat caccaatgaa
    59701 attatgcaat tatatacgga tagggaggtt cttaacatgg ggctttgtag tcgttataaa
    59761 agtcttacat gcaatagttg ctcaatgcat tgccaaataa tgccagaaga gtcaccgcgt
    59821 ttgcagtact gcgcaaactc gtgtt
//
LOCUS       DAEEGA010000014        58601 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEEGA010000014 DAEEGA010000000
VERSION     DAEEGA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58601
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.014"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..1128)
                     /locus_tag="KLH46_000883"
     CDS             complement(<1..1128)
                     /locus_tag="KLH46_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBE0377675.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALVGNAANLLI"
     gene            complement(1340..1687)
                     /gene="pspE"
                     /locus_tag="KLH46_000884"
     CDS             complement(1340..1687)
                     /gene="pspE"
                     /locus_tag="KLH46_000884"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBE0377676.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1730..1951)
                     /gene="pspD"
                     /locus_tag="KLH46_000885"
     CDS             complement(1730..1951)
                     /gene="pspD"
                     /locus_tag="KLH46_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBE0377677.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1960..2319)
                     /gene="pspC"
                     /locus_tag="KLH46_000886"
     CDS             complement(1960..2319)
                     /gene="pspC"
                     /locus_tag="KLH46_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBE0377678.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2319..2543)
                     /gene="pspB"
                     /locus_tag="KLH46_000887"
     CDS             complement(2319..2543)
                     /gene="pspB"
                     /locus_tag="KLH46_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBE0377679.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2597..3265)
                     /gene="pspA"
                     /locus_tag="KLH46_000888"
     CDS             complement(2597..3265)
                     /gene="pspA"
                     /locus_tag="KLH46_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBE0377680.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3432..4409
                     /gene="pspF"
                     /locus_tag="KLH46_000889"
     CDS             3432..4409
                     /gene="pspF"
                     /locus_tag="KLH46_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBE0377681.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4529..5794)
                     /gene="puuE"
                     /locus_tag="KLH46_000890"
     CDS             complement(4529..5794)
                     /gene="puuE"
                     /locus_tag="KLH46_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HBE0377682.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5832..7112)
                     /gene="puuB"
                     /locus_tag="KLH46_000891"
     CDS             complement(5832..7112)
                     /gene="puuB"
                     /locus_tag="KLH46_000891"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBE0377683.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7114..8601)
                     /gene="puuC"
                     /locus_tag="KLH46_000892"
     CDS             complement(7114..8601)
                     /gene="puuC"
                     /locus_tag="KLH46_000892"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HBE0377684.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8751..9308)
                     /gene="puuR"
                     /locus_tag="KLH46_000893"
     CDS             complement(8751..9308)
                     /gene="puuR"
                     /locus_tag="KLH46_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBE0377685.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9335..10099)
                     /gene="puuD"
                     /locus_tag="KLH46_000894"
     CDS             complement(9335..10099)
                     /gene="puuD"
                     /locus_tag="KLH46_000894"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBE0377686.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10311..11729
                     /gene="puuA"
                     /locus_tag="KLH46_000895"
     CDS             10311..11729
                     /gene="puuA"
                     /locus_tag="KLH46_000895"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HBE0377687.1"
                     /translation="METNIVEVENFVQQSEERRGSTFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11878..12042
                     /locus_tag="KLH46_000896"
     CDS             11878..12042
                     /locus_tag="KLH46_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377688.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12032..13417
                     /gene="puuP"
                     /locus_tag="KLH46_000897"
     CDS             12032..13417
                     /gene="puuP"
                     /locus_tag="KLH46_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HBE0377689.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13551..13796
                     /locus_tag="KLH46_000898"
     CDS             13551..13796
                     /locus_tag="KLH46_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBE0377690.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14109..15752
                     /gene="sapA"
                     /locus_tag="KLH46_000899"
     CDS             14109..15752
                     /gene="sapA"
                     /locus_tag="KLH46_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBE0377691.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15749..16714
                     /gene="sapB"
                     /locus_tag="KLH46_000900"
     CDS             15749..16714
                     /gene="sapB"
                     /locus_tag="KLH46_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBE0377692.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16701..17591
                     /gene="sapC"
                     /locus_tag="KLH46_000901"
     CDS             16701..17591
                     /gene="sapC"
                     /locus_tag="KLH46_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBE0377693.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17591..18583
                     /gene="sapD"
                     /locus_tag="KLH46_000902"
     CDS             17591..18583
                     /gene="sapD"
                     /locus_tag="KLH46_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBE0377694.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            18585..19391
                     /gene="sapF"
                     /locus_tag="KLH46_000903"
     CDS             18585..19391
                     /gene="sapF"
                     /locus_tag="KLH46_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBE0377695.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19459..19812
                     /locus_tag="KLH46_000904"
     CDS             19459..19812
                     /locus_tag="KLH46_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBE0377696.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20180..20968
                     /gene="fabI"
                     /locus_tag="KLH46_000905"
     CDS             20180..20968
                     /gene="fabI"
                     /locus_tag="KLH46_000905"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBE0377697.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21112..22239
                     /locus_tag="KLH46_000906"
     CDS             21112..22239
                     /locus_tag="KLH46_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBE0377698.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22307..24241
                     /gene="rnb"
                     /locus_tag="KLH46_000907"
     CDS             22307..24241
                     /gene="rnb"
                     /locus_tag="KLH46_000907"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBE0377699.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24319..24421)
                     /locus_tag="KLH46_000908"
                     /pseudo
     CDS             complement(24319..24421)
                     /locus_tag="KLH46_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24475..26449
                     /gene="pdeR"
                     /locus_tag="KLH46_000909"
                     /pseudo
     CDS             24475..26449
                     /gene="pdeR"
                     /locus_tag="KLH46_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26597..26770
                     /locus_tag="KLH46_000910"
     CDS             26597..26770
                     /locus_tag="KLH46_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377700.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26860..27609
                     /locus_tag="KLH46_000911"
     CDS             26860..27609
                     /locus_tag="KLH46_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE0377701.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27878..28096
                     /gene="osmB"
                     /locus_tag="KLH46_000912"
     CDS             27878..28096
                     /gene="osmB"
                     /locus_tag="KLH46_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBE0377702.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28222..28548)
                     /gene="yciH"
                     /locus_tag="KLH46_000913"
     CDS             complement(28222..28548)
                     /gene="yciH"
                     /locus_tag="KLH46_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBE0377703.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28548..29285)
                     /gene="pyrF"
                     /locus_tag="KLH46_000914"
     CDS             complement(28548..29285)
                     /gene="pyrF"
                     /locus_tag="KLH46_000914"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBE0377704.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29478..30647)
                     /gene="lapB"
                     /locus_tag="KLH46_000915"
     CDS             complement(29478..30647)
                     /gene="lapB"
                     /locus_tag="KLH46_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBE0377705.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30654..30962)
                     /gene="lapA"
                     /locus_tag="KLH46_000916"
     CDS             complement(30654..30962)
                     /gene="lapA"
                     /locus_tag="KLH46_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBE0377706.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31111..31875)
                     /gene="pgpB"
                     /locus_tag="KLH46_000917"
     CDS             complement(31111..31875)
                     /gene="pgpB"
                     /locus_tag="KLH46_000917"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBE0377707.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32045..32635
                     /gene="ribA"
                     /locus_tag="KLH46_000918"
     CDS             32045..32635
                     /gene="ribA"
                     /locus_tag="KLH46_000918"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBE0377708.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32699..35374)
                     /gene="acnA"
                     /locus_tag="KLH46_000919"
     CDS             complement(32699..35374)
                     /gene="acnA"
                     /locus_tag="KLH46_000919"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBE0377709.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35538..35633)
                     /locus_tag="KLH46_000920"
     CDS             complement(35538..35633)
                     /locus_tag="KLH46_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377710.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35747..35914)
                     /locus_tag="KLH46_000921"
     CDS             complement(35747..35914)
                     /locus_tag="KLH46_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377711.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35917..36081)
                     /locus_tag="KLH46_000922"
     CDS             complement(35917..36081)
                     /locus_tag="KLH46_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBE0377712.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36376..37350)
                     /gene="cysB"
                     /locus_tag="KLH46_000923"
     CDS             complement(36376..37350)
                     /gene="cysB"
                     /locus_tag="KLH46_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBE0377713.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37560..40157)
                     /gene="topA"
                     /locus_tag="KLH46_000924"
     CDS             complement(37560..40157)
                     /gene="topA"
                     /locus_tag="KLH46_000924"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBE0377714.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILCFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDTYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40537..40788
                     /locus_tag="KLH46_000925"
     CDS             40537..40788
                     /locus_tag="KLH46_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBE0377715.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40824..41873)
                     /gene="sohB"
                     /locus_tag="KLH46_000926"
     CDS             complement(40824..41873)
                     /gene="sohB"
                     /locus_tag="KLH46_000926"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBE0377716.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42093..42851
                     /locus_tag="KLH46_000927"
     CDS             42093..42851
                     /locus_tag="KLH46_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBE0377717.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42848..43438
                     /gene="cobO"
                     /locus_tag="KLH46_000928"
     CDS             42848..43438
                     /gene="cobO"
                     /locus_tag="KLH46_000928"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBE0377718.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43478..44350)
                     /gene="rluB"
                     /locus_tag="KLH46_000929"
     CDS             complement(43478..44350)
                     /gene="rluB"
                     /locus_tag="KLH46_000929"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBE0377719.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44451..45071)
                     /locus_tag="KLH46_000930"
     CDS             complement(44451..45071)
                     /locus_tag="KLH46_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBE0377720.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45068..45949)
                     /gene="yciV"
                     /locus_tag="KLH46_000931"
     CDS             complement(45068..45949)
                     /gene="yciV"
                     /locus_tag="KLH46_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBE0377721.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46087..46131
                     /gene="trpL"
                     /locus_tag="KLH46_000932"
     CDS             46087..46131
                     /gene="trpL"
                     /locus_tag="KLH46_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBE0377722.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46108..46201
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46223..47785
                     /gene="trpE"
                     /locus_tag="KLH46_000933"
     CDS             46223..47785
                     /gene="trpE"
                     /locus_tag="KLH46_000933"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBE0377723.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47785..49380
                     /gene="trpD"
                     /locus_tag="KLH46_000934"
     CDS             47785..49380
                     /gene="trpD"
                     /locus_tag="KLH46_000934"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBE0377724.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49381..50742
                     /gene="trpCF"
                     /locus_tag="KLH46_000935"
     CDS             49381..50742
                     /gene="trpCF"
                     /locus_tag="KLH46_000935"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBE0377725.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50754..51947
                     /gene="trpB"
                     /locus_tag="KLH46_000936"
     CDS             50754..51947
                     /gene="trpB"
                     /locus_tag="KLH46_000936"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBE0377726.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51947..52753
                     /gene="trpA"
                     /locus_tag="KLH46_000937"
     CDS             51947..52753
                     /gene="trpA"
                     /locus_tag="KLH46_000937"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBE0377727.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53134..53313
                     /locus_tag="KLH46_000938"
     CDS             53134..53313
                     /locus_tag="KLH46_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBE0377728.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53399..53899
                     /locus_tag="KLH46_000939"
     CDS             53399..53899
                     /locus_tag="KLH46_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBE0377729.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53945..54451
                     /locus_tag="KLH46_000940"
     CDS             53945..54451
                     /locus_tag="KLH46_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBE0377730.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54511..55149)
                     /gene="ompW"
                     /locus_tag="KLH46_000941"
     CDS             complement(54511..55149)
                     /gene="ompW"
                     /locus_tag="KLH46_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBE0377731.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55389..55478
                     /locus_tag="KLH46_000942"
                     /pseudo
     CDS             55389..55478
                     /locus_tag="KLH46_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55506..56249
                     /locus_tag="KLH46_000943"
     CDS             55506..56249
                     /locus_tag="KLH46_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBE0377732.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56279..56818
                     /locus_tag="KLH46_000944"
     CDS             56279..56818
                     /locus_tag="KLH46_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBE0377733.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56923..57321
                     /gene="yciA"
                     /locus_tag="KLH46_000945"
     CDS             56923..57321
                     /gene="yciA"
                     /locus_tag="KLH46_000945"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBE0377734.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57361..58089)
                     /gene="tonB"
                     /locus_tag="KLH46_000946"
     CDS             complement(57361..58089)
                     /gene="tonB"
                     /locus_tag="KLH46_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBE0377735.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58304..58600
                     /locus_tag="KLH46_000947"
     CDS             58304..58600
                     /locus_tag="KLH46_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBE0377736.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14067 a        15154 c        14652 g        14728 t
ORIGIN      
        1 aatcagtaag ttggcagcat tacctactaa tgcaccttcc tgctgtaatg cctcgaccag
       61 cgctaatatt tcgctttctg gaagcaagcc acgcagtggg ttcaacccga tgccatcgtg
      121 agaggcgagg aagttaaacc aggtcgtttt actggagggc aagctcagac tttgcgccca
      181 ctggcacagc gcctcgacgt tctgtttttg caccgcatgt aacaccagcg gcggcagcga
      241 gaactggtac accatatgcg cttcgtcatc accgttgcca aagtaagcaa tgttgtcttt
      301 gtgcggaaca ttggtttcgg taatgatcac tgtacctggt gctacgtcat caataatcga
      361 ccgtaacagc ttgataatca gatgtgtttt ttccagatgg atgcagcttg ttcccggctc
      421 tttccacata aagccaacgg catccaggcg gacatactca gcacctttct caaggtaaca
      481 gagtaaaaca tccaccatcg ccagcaacac ttcagggcta cggtaattca ggtcaatttg
      541 atcgtcacta aaggtggtcc ataaatggcg cgtagaatta tcatccattt gaaacggcgt
      601 taatagcggt aacgcacgcg gaagggtgac ggcactgaga tcggttttcg gatcaaccgc
      661 aataaagaaa tcttcaaaac ccggaagctg ttgtaaatag tttttaaacc attcactttt
      721 tgcagacata tggttgcaga caaaatcaaa cattaaatga ctgcattcac cgagttgctg
      781 aatatcctgc cactcccccg cttcactggc gacctgatga taatcaatta ccgaaaatcc
      841 atcatcagat gaccatgggt aaaaaggtaa taaatgaaca tgtgaaaaaa tgctttgcag
      901 ccattgatgg tagaactgat taaatatggg tagtggtttt aaatcattac tgtgaaattg
      961 atcggcatag gtaattaaca cgacatcact ttcatcccag tgttttttac gtcgctgtgt
     1021 aattaatcgt ttcgcactct caagacgcgt tacaagtttc tgtaaatgag ttgcggtaaa
     1081 tgttccaccg tagatttcgt ccatgtaatc cgtaattttc tgtttcatgt tgcctttccg
     1141 tggtagcggt cccatgaggc gagagactac tccgcagaaa ataaactgtc aattgagcac
     1201 aagggtttta gtcacgcagg aaatcatttt ttaccaggga aaaagcgtat ttgtgagctg
     1261 acgcaaataa tctataggga tgtaaataag gtgtgacaga aaaaaaacgg cgcataagcg
     1321 ccgctcatgg tgaattcttt taacctttga ccttcggcat tgcgatgtct ttcaggccac
     1381 cggcgttctc aacgtgggta tatcccatct cgctaaggag ctcttttgcc tgcccggact
     1441 ggcgtccggc attgcaatac actttcacgg tgtcgttttt atccggaacg gcggtggcaa
     1501 tgcgctcttt cacttccttc aggggaatat tgatggcccc ctgaacgtgc tcttgctgat
     1561 actgctctgg aacacgaaca tcgatccagt gttcagcggc gaaaacgggc agtgaaaaca
     1621 ccagtgccag agcaagtaag ccttttttta aacatgtaag cgtccttcta tggatgaaaa
     1681 ttgccatcca ttatacgcct gagatttaac aatttttgcg aaactcccct caccttttat
     1741 aacgctgtgc cagtttatta gcagcccgac ttaacagcgg ttccagcgcc acagccagca
     1801 acattttcag cggtcggcga gcaactgatt ttatcgccca gcccgccaca cccgccgggc
     1861 catagcgcag tgcggtaaga agtaccagct tgcctgctaa tttgaaacca agcttcacct
     1921 tttgcccggc ctgttgccag cgagtattca taactttcct cacagttgac ggaaacggct
     1981 acgtaacgtg aaagtatcgg aagtgacata acgttccatc tcgcgtaaac gcgtttcact
     2041 tgccgccagt tcacggtcga cttcatccag caattcgctg ctggaaggta gctgctcacc
     2101 aaaggccatg ttgtccggca ttggatcaag cgcaaatgac aaaatgatgt aagcaaccag
     2161 ggtaaacagc gccagaccga agaaaatcga cagcaccacc aggatacgca ccagttttac
     2221 cggtacatca aaatagttgg caatcccggc gcagacgccg cggaccatgc cctgctgtgg
     2281 aatacgccat aactttttat tgagattaat gcccgccatt agcgatccct ccagttcgga
     2341 tgttctgcat caagaataga ttccagcgcc tgaatacgtt cgcgcatccg ttttgcttca
     2401 tcaaccagtt gcgctaatcg ctgctgctca ctttgcgaca attcactgcg accagaacga
     2461 ttgctgtaat gcagccataa ccagatcggt aaaacaaaca gcacaaaaat ggttaacgga
     2521 atagccagaa atagcgcgct cataagtact ccttagacga tgagcggcgg cgcgttcgga
     2581 cgccgcctgg atgttattat tgattgtctt gcttcatttt ggcttttaat tgtgccagtt
     2641 gttcgctgat tgcatcatcg gctttcagtt cggcaaactg atcgtccagc gatttttgtt
     2701 taccgaagct gtggctttct gcttccgctt ccatctggtc aatacgacgt tcgaaagatt
     2761 cgaaacgagc cattgcttca tccagtttgc cactgtccag ctgacgacgc acatcgcgcg
     2821 acgagtttgc cgcctgatga cgtaacatca atgcctgctg gcgagcgcgt gtttcgctca
     2881 atttgttttc cagctcgcca atctctttct tcatgcgtgc cagcgtatcg tccaccagcg
     2941 tcacttcatg ttccagggac ttaatcagat cggtcagttt ctgtttttca attaacgctg
     3001 cacgtgccag atcctctctc tctttcagca gcgccagttc ggctttttcc tgccattcaa
     3061 cttcacgcgc cgacgcttgt tcaatacggc gagtcagctg tttcttttct gccaacgcac
     3121 gcgccgaagt agaacgtact tcaaccagtg tatcttccat ctcctggatc atcagacgca
     3181 ccagtttctg tggatcttcc gctttctcta acagagcgtt gatgttggca ttcacgatgt
     3241 cggcaaagcg agaaaaaata cccataatgt tgtcctcttg atttctgcgt tcaggattgt
     3301 cctgctgaac tgttaatgca atttgcgtgc caattttttg tctttttgat ttataaagat
     3361 ctgattgaag aatcaacagc aacatgccag gatgaattag cgaattacac taacaagtgg
     3421 cgaatttcat catggccgaa tacaaagata atttacttgg tgaggcgaac agctttctcg
     3481 aagtgctgga acaggtttcg catctcgcac cgctggacaa accggtgctc atcatcggcg
     3541 aacgcggcac cggtaaagag ttgattgcca gccgcctgca ttatctctcc tcccgttggc
     3601 aagggccgtt tatttccctt aactgcgcgg cgttaaatga aaatctgctg gattccgaac
     3661 tgtttggtca cgaagcgggg gcgtttaccg gtgcgcaaaa acgtcatcct gggagatttg
     3721 aacgtgccga cggcggtacg ctatttcttg atgaactcgc tacggcaccg atgatggtgc
     3781 aggaaaaatt attgcgtgtg attgagtacg gtgaactgga gcgcgttggc ggtagccagc
     3841 cgttgcaggt gaatgtgcgg ttggtatgcg cgacgaatgc cgatctcccg gcgatggtca
     3901 atgaaggtac ttttcgcgct gacctgctcg accggctggc tttcgatgta gtgcaactgc
     3961 cgcccctgcg cgagcgcgaa agcgacatta tgctgatggc agaacacttt gccattcaga
     4021 tgtgtcggga aatcaagctg cctctgttcc cggggtttac ggagcgcgcc agagaaacat
     4081 tgctgaatta tcgttggccg ggaaatattc gtgaattgaa aaacgtggtg gaacgttcag
     4141 tgtatcgcca cggcaccagc gattatccgc ttgatgacat cattattgat ccctttaaac
     4201 ggcgtccacc tgaagaagcc atcgccgttt cagaaaatac ctcgcttccg acgctgccgc
     4261 tggatttacg cgagtttcag atgcagcagg aaaaagagtt gctgcaactc agtttgcaac
     4321 aggggaaata taaccagaag cgcgcggctg aattactggg gttaacttat catcagttcc
     4381 gcgcgttgtt gaaaaagcac cagatttagc gaagttgagc aatacaactt gccggatgcg
     4441 gcgtgaatgc tttattcggc atacataatc aatacgtcgt ttgcaggcct gataagcgca
     4501 gcgcatcaga cattattgcg ttgggctatt aatcgctcag cgcatcctgc aaaattttca
     4561 ttgccgcatc gaattgcgca tccgggatgg tcagcggata caggaagcga atcacgttgc
     4621 cgtatgcgcc acaggtcagc aggagcagcc cctgcgccag cgcgcgttgc tggattttct
     4681 gtgcaatcgc cgctgacggc tcgcccgttt gcggatcgtt aaactctgcc gcaatcatcg
     4741 accccaggcc gcgtaccgca gcaatggccg gaacgctttc tttggcatca atcaacgtgt
     4801 ttttgagacg ctggcccagt tgattcgcgc gttcgcagag tgattctttg tcgataatat
     4861 tgagcaccgc gtgcgcggca gccaccgcca gcgggttccc ggcgtaggtg ccacccaacc
     4921 cgcccggcgc gggcgcgtcc ataatattcg cgttaccgac cacgcccgaa agcggcatcc
     4981 cgcccgcgag gcttttcgcc atcgtcatta agtccggctt atcggcgtaa tgatccatgg
     5041 caaacagttt accggtacgc gcaaagccgc tttgcacttc atcggcaatc atcacaatac
     5101 cgtgctcgtc gcacaggcgg cgaatggcgg caaccagctc ttttggcgca acgttgaaac
     5161 cgcactcgcc ctgcaccggt tcgaaaataa tggccgcgac ctgcttcgct tcgatgtctg
     5221 atttaaacaa gcgttcgatg gcgtcgaggg agtcctgtgt tgaaatgccg tgtaaatctg
     5281 acggataagg tacgtgatac accgaaccag ggaacgggcc gaagccgatt ttgtacggcg
     5341 caacttttcc ggtcagcgcc atggtcatat atgtacgacc gtggaagcca ccgctaaacg
     5401 caatcacgcc aggtcgtccg gtatgggcgc gagcaatttt caccgcgttt tccaccgctt
     5461 ccgcgccggt agtgaagaat gcggttttag cctgcccgct cactggggca agggtgttaa
     5521 ttttctccgc cagcgtgacg tagctttcgt acggcacaat ctgatacgcc gtgtgggtaa
     5581 actgttgcaa ttgctgctcc accgccgcga ccagatcagg gtggcgatgc ccggtattca
     5641 gcaccgcaat gcctgcggcg aaatcgatgt attcgttgcc ctctacgtcc ttcagcgtag
     5701 cgttttctgc cgactgggcg aagaagttac acatcacgcc aaccccgcgt ggggtagcag
     5761 aaagacgacg ctgatggaat tcattgttgc tcatgtttca caccttttga ctcttcactg
     5821 ttaatcggaa attaaacgcc cagacgatcg cgcaggctgt aataagccgc gcccatcgcg
     5881 gtaaatggca cgcgcagcgt gcgcccgccg gggaacgggt aatgcggcag attggcgaag
     5941 gcatcgaaac gctcggcgtc accgcgcagc agttcagcaa tcaaacgccc ggcaagatgg
     6001 gtacaggtga cgccgtggcc gctgtaaccc tgcatgtaat agatgttggt atcaaggcgg
     6061 ccgaactgcg gcatgtgcga caaggtcagc aggaagttgc ccgtccagcg gtaatcaatt
     6121 ttcacgccct tcaactgagg gaaggttttt agtagtttcg gtaccacaag acgctcaacg
     6181 tcatccgggt tgcgtgcacc atacactaca cccccgccgt acagcaggcg gttgtcggcg
     6241 gtaagacggt agtaatcaag cagatagtta cagtcttcca cacagtagtt tttcgggatc
     6301 agcgaacggg ctaaatcttc cgacagccgt tcggtggtga tcacctgcgt gccgcacggc
     6361 atgctgcgtt tcgctagttc cggctctact ttatcgccca gatacgcatt cccggcgaca
     6421 atcacatact tcgccgtcac ctgaccattg gcagtccgaa ccacggctgg cgtggtgtgc
     6481 tggatctgcg tcacggcgga aagctcatac acgcgcccgc cgttcaggcg gatggcatcg
     6541 gcttcaccaa tggcgaggtt tagcgggtga atatgcccac cgctgtgatc cagcagcgca
     6601 ccagtgtagc gatcgctggc gacctcgcgg cgaatagcgt tgacgtccag cagctccagc
     6661 tgtttattgc cgtagcgttc ccagttctct ttctgctctt cgagtgtggc gagttgttta
     6721 tcattcatcg ccacaaacag accgccgggg cggtagtcgc agtcaatctg ataacgtttg
     6781 atacgttcgc ggatgatctc gccaccctcg aacatcatgc tgccgagcat acgggcggtg
     6841 tccatgccgt agcttttttc gatcacgtcg atgtcgcggc tgtaggagtt cacaagctgc
     6901 ccgccattgc gcccgcttgc gccaaagccg atgcgtgagg cttcgagaac cactacgtca
     6961 aagcccgctt ccgccagatg cagcgcggag gagagcccgg tatagccgcc gccaaccacg
     7021 caaacgtcgc aggtgattga ctcattcagt gtgtcgaatg gtgcatattt attcgcactg
     7081 gcggcgtagt aactgctggt atgttcggtc atttcaggcc tccaggctta tccagatggt
     7141 tttcagttca gtgaattttt caagggcatg cagggatttg tcgcgaccgt tgccgctctg
     7201 cttatagccg ccaaacggca cggtcatatc gccgtcgttg tagttattga cgaagacgga
     7261 accggctttc aggcgtcggc tcattcggtg cgcgcgggag aggtcgcgcg tccataccgc
     7321 cgcgccaagg ccgtactggc tgtcgttggc aagctgcagc gcctgatctt ctgatgtgaa
     7381 acgggtgacc accagcaccg gaccgaaaat ctcttcgcgg cttaaggacg cattcgggtc
     7441 cacatccacg aagatggtcg ggccgatggc cgctgccagc ccggcgttgc gaccatccag
     7501 cagcagttgc cccttgcctt cgccttcacg aataaaggtg tgtaccgagt cagcgtgagc
     7561 gctgtcgatt aaggtgccca tggtggttgc gggatcaagt ggatggcccg gctgccagtt
     7621 ttgcgcctgc tgttttaaca gggctaagaa ttcatcggcg atgctctctt ccagtaacaa
     7681 acgcgttccg gcgatgcaca cctggccctg gttgtagaag atgcctgcgg cggtggcgct
     7741 tgccgctttt tgcagatccg gacaatcggc gaaaacgatg ttggcgcttt tgccgcccgc
     7801 ttccagccag acacgtttca tgttgctatc acccgcgtct ttcagcagtt gtttacccgt
     7861 acgcgtggag ccggtaaagg cgatggcatc gatatcgtta tgacgcgaaa gcgcctgccc
     7921 cgcttcatga ccaaaacccg tcaccacgtt caacacacca tccggcaagc ctgcttcttt
     7981 cgccagcccc gcgagacgaa tcgcactgag cggtgatttt tcagacggtt ttagaatcac
     8041 gctgtttccc gccgccagcg ccgggccgag tttccagcaa gtcagcaaca gcgggaagtt
     8101 ccacggcacg atggcggcaa tcacgccgac cggttcacgc acgatcatcg ccagctcatg
     8161 gctactggtg gtcgccactt cgccatacac tttgtcgatc gcttcggcgt accagcgaat
     8221 ggcgcgcgcc gcgccgggaa tatcatcacg cagactgtga cgaatcggtt tgccggtgtc
     8281 gagagtttcc agcagtgcca gctcttcggc gtgggcttcc attaaatcgg cgagtttatt
     8341 cagtaccgct ttacgtttag ccggagaaga gagtgaccag tcgccgcgtt caaatacgcc
     8401 gcgtgctgcg ctcaccgcac ggtcgatatc gacgctcttg ccgcgggcaa ttttcgccag
     8461 cggtgcctgg gtgaccggat caacggtttc aaaggtttca ttttccgccg cagcagtata
     8521 ttcaccgtta ataaataagc ggttttcaat ggcgagactt aacgctttat cctgccagta
     8581 agccagatga tgaaaattca ttatgactcc tgtttcacgt ctatcagata tatgcagttt
     8641 gtgaacgtca gggcgcagag atccccgggc tgcatgcccg tttatttctg gcgacattct
     8701 ttatggattg ccgggcgcat tgcgcttacc cggcttacag aacaaaaaga ttaaaacgtg
     8761 gtgggcgtat gggcgctgat aattcggcaa atacctgccg acgtattact gaaactgtgc
     8821 gggatgccgg tattaatggc ataactttgc cccgcgacga ggtggtaatc ctgaccatta
     8881 atcgtcagaa caatttcacc ttccagtact gtgcctattt cctcaccctg atgcttaatt
     8941 ctttccccag tggttgtgcc cggctggtac gtttcaaaga tcatcgccag cgtgcgattc
     9001 gggttaccgt tatgaaccag cttcattgac acaccctgac tgcccatctc aattaagtcg
     9061 tcctgattaa tgacgacctg cggctcatca ggtttttccg gctcggaaaa gaattccgag
     9121 agtgacaaac catacacctt cagcagcttt tgcagcgtac tgatggcagg gctgacttta
     9181 tcttgttcta tcgtactgat agcactgtga gtcagcccgg agagttcggc ggcacgacgt
     9241 tgtgaaagcc cctgctgctg gcggatttcc gacaagcgtt ttcctggcgc cagtccctca
     9301 tcactcatat ttgcatttcc ttaaactgta gtggtcagag tcgctgtttt tcagcgatat
     9361 ggtgctgcca agcggtgata aagccctcga acaatatacg cgaaagcgcg tactcgctac
     9421 tgttccattc cgggtgccac tgtacgccca gcgcaaaagg atgattgatg acgctaaccg
     9481 cctcaactaa gccatccggc gagcgagctt caacacgcaa ccgtgggcta acgaccttcg
     9541 ccccttgtcc atgtagagag tttacccaaa agttgctaca ttcaggtaac aacgcagaca
     9601 gtaatccccc ctcttcaacc tgaacttcat gcgacggtgc atattgctgt tccaccggca
     9661 gttcgggatc ttcccgatgt tccagcaatt caggctgttc gcacagcttg cgatgcaacg
     9721 aaccaccggt tgccaccacc agttcttgta aaccccggca gatggcgaaa atggggatgc
     9781 gcctttcgag tgcggcatta attatcgcca tgctcagaag atcacgcccg ggatcggcgt
     9841 caggctcatc gccgttttca ccatataggt gcggctgcac attgctggga ctactaggaa
     9901 gataaatgcc atcgagtttc ggcaaaagtt gttcaagtaa cgacggttcc gccagcgcat
     9961 gtggtagcgc aattggcaag ccgcctgcgt ggatgatggc attcaggtac ttttcttgca
    10021 gagtctgggt cgcatgaccc ttaagcctgt tcctgcacat tacgacaccg ataaccggat
    10081 tgttcattat attttccatg ctcaatctca caaagtggac taaattatcg ccattactgc
    10141 gctttaaatg acctttatgt tcaatatttt ttcaatctag cagtggattt tcatttttgc
    10201 aaactcaatt taacatttga caaacatttg gtttgcatac agattcgaat gatggtcatt
    10261 atattttacg ctttgataac gagcggaaaa caaaccaaag gcgaagaatc atggaaacca
    10321 atatcgttga agtagagaac tttgttcagc agtcagaaga gaggcggggt agcaccttta
    10381 cgcaggaagt gaaacgctac ctggagcgct acccgaatac gcaatatgtt gatgttttac
    10441 ttactgattt gaatggttgc ttccgtggaa aacgtatccc tgtttcaagc ctgaagaagc
    10501 tcgagaaagg gtgttatttc ccggcctcgg tatttgcaat ggatattctg ggcaacgtag
    10561 tagaagaagc aggtctgggt caggaaatgg gcgagccgga tcgtacctgt gttcctgttc
    10621 tcggttcctt aactccttct gccgccgatc cagagtttat cggtcagatg ctcctgacca
    10681 tggtcgatga agatggcgct ccctttgacg ttgagccgcg gaacgttctt aaccgcctgt
    10741 ggcagcagct gcgccagcgc ggattgttcc cggttgtagc ggtagagctg gagttctatt
    10801 tactggatcg ccagcgcgac gctgaagggg atctgcaacc gccctgcgcg cctggcaccg
    10861 atgaccgcaa tacacaaagc caggtttact ccgttgataa cctcaaccac ttcgccgacg
    10921 tgctcaatga tattgatgaa ctggcgcagt tgcagctgat tccggcagat ggcgcggtcg
    10981 ctgaggcctc gccgggtcag tttgaaatca acctttacca tactgataac gtgctggaag
    11041 cctgcgatga tgcgctggca ctaaaacgcc tagtgcgtct gatggcagaa aagcataaga
    11101 tgcacgccac ttttatggcg aagccgtatg aagagcacgc gggcagcggg atgcatatcc
    11161 atatcagtat gcaaaataat cgtggcgaga atgtgctttc tgacacagaa ggcgaagatt
    11221 cgccgctgct gaaaaagatg ctcgccggga tgattgacct gatgccgtcg tcgatggcgt
    11281 tgctggcacc aaacgtgaac tcgtatcgcc gcttccagcc gggaatgtat gtgccgacgc
    11341 aggcgtcgtg gggccataac aaccgcaccg tcgccctgcg tattccgtgc ggtgaccgtc
    11401 ataatcaccg cgtggaatat cacgtggcgg gtgccgatgc caacccatat ctggtgatgg
    11461 cagcgatttt tgccggtatt ttgcatggcc ttgataacga gctgccgttg caggaagaag
    11521 tcgaaggcaa cgggctggaa caggaaggct tacccttccc gattcgccag agcgatgccc
    11581 tgggtgagtt tatcgagaat gatcacctgc gccgctattt aggcgaacgg ttttgccatg
    11641 tgtatcacgc ctgtaaaaat gatgagctgt tgcagtttga gcgcctcatc acagaaacag
    11701 aaattgagtg gatgttgaaa aacgcgtaat tccccttgcg gggttgtttc atcggcctga
    11761 gcggagccag gcctgacatt tgctggtaat ccagacattt cccggatgtc gcgttatgga
    11821 gcgcaggcgg caacgggctt ccgttaacga ttaagactgt gacgaggaaa tgagatgatg
    11881 ccgatgatta aatccccgca tggcgaggga ggctgcgtat gcgcccctcc tgctactgac
    11941 tggactccgc cccctttgct tcccttgcta aacaggtttg atttcaggtc gacacgaccg
    12001 caaaccttat tacgcagggg aggcagcaat tatggctatt aattcaccac tgaatattgc
    12061 tgcgcaaccc ggcaaaaccc gtctgcgaaa atcactgaaa ttgtggcagg tggtaatgat
    12121 gggtctggcc tatctcacgc cgatgaccgt atttgatact tttggtattg tctccggcat
    12181 tagcgacggt cacgttccgg cgtcctattt gctggcgctg gcgggcgtgc tgtttaccgc
    12241 tatcagctac ggcaaactgg ttcgccagtt tccggaggcc ggttcggcct atacctacgc
    12301 gcaaaagtcg attaacccgc acgtcggatt tatggtcggc tggtcatcac tgctggatta
    12361 tctctttttg ccaatgatca acgttttgtt ggcgaaaatc tatctctccg ccctcttccc
    12421 ggaagtgccg ccgtgggtgt gggtggtaac cttcgtcgcc attttaaccg ccgcgaatct
    12481 gaagagcgtc aacctggtcg ctaacttcaa taccctgttt gtactggtgc aaatctccat
    12541 catggtggtg tttatcttcc tggtggttca gggactgcat aaaggagaag gcgttggcac
    12601 cgtctggtca cttcagccgt ttatcagcga gaacgcgcac ctgatcccga ttattaccgg
    12661 tgcgacgatt gtctgtttct cgttcctcgg tttcgatgcg gtgaccacgc tttcggaaga
    12721 gacgccggac gccgcacgcg tgatcccgaa agccatcttc ctgacggcgg tatacggcgg
    12781 cgttatcttt atcgcggcgt cgttctttat gcagctgttc tttcccgata tcagccgctt
    12841 taaagacccg gacgccgcac tgcctgaaat tgcgctgtac gtcggcggca agctctttca
    12901 gtcgattttc ctctgcacca cgtttgtgaa cacattagcg tctggcctgg cttcacatgc
    12961 cagcgtgtcg cgtctgctgt atgtgatggg gcgtgacaat gtgtttccgg agcgcgtgtt
    13021 tggctatgtg cacccaaaat ggcggactcc ggcactcaac gtcattatgg tcgggattgt
    13081 cgcgctgtcg gcgctgttct ttgatttagt caccgcgaca gcactgatta acttcggtgc
    13141 tctggtggcg tttaccttcg tgaatctgtc ggtgtttaat catttctggc ggcgtaaagg
    13201 aatgaataaa agctggaagg atcacttcca ctatttgctg atgccgctgg ttggcgcgct
    13261 gacggtgggt gtgctgtggg ttaacctaga gtcaacgtca ctgacactcg gtctggtatg
    13321 ggcttcgtta ggcggcgcat atttgtggta tctgatccgc cgctatcgca aagtgccgct
    13381 gtacgaaggt gacagaacgc cggtgagcga aacgtaatag ccggatgcgc ggcgcattat
    13441 cctgcctgag ggtaacgcgc cgcgccatta actataaaaa gatgtatgac ccggatcacg
    13501 agtgctatca tccgccctta tgcttagcgc attaacccca ggggcaaata atgaaccacg
    13561 atatcccgct aaaatatttt gatattgccg atgagtacgc gaccgaatgc gcagagcctg
    13621 tcgcagaggc tgaacgcaca ccgctggccc actacttcca gttactgctc acccgtttga
    13681 tgaacaatga agagatcagc gaagaagccc aacatgaaat ggctgctgaa gcggggatta
    13741 atcccgtacg cattgatgag atcgcagagt ttttgaatca atggggtaat gagtaattat
    13801 ttaattgcag acgatcggtc agtatcgttg tccggataaa gcgttcacgc cgcatccggc
    13861 taacaatgac acgcactgca gcgtgcttta atgggtttct cacgatcgta ccgctctgct
    13921 cctcgctaag ttgttctgtg agttatagtt atagagaaat cacgggtacg aatgttgaac
    13981 agatggcgac gccacctgtt caacgaccaa ccacacggcc ctcattacga cccctgaaca
    14041 caaacggtaa tggcaaatgt cattaaatat tagtaagata gcctctttac ataaaatccc
    14101 ttaatattat gcgccaggta ttatcgtctc ttttggtgat tgctggactt gtgagtggtc
    14161 aggcaatcgc cgcgcctgaa tcccccccgc atgctgatat ccgcgacagc ggttttgtct
    14221 attgcgtcag cgggcaagtc aacaccttta acccatccaa agcgagcagt gggttaattg
    14281 tcgataccct tgccgcccag ttttatgatc gactgctgga tgtcgatccc tatacctatc
    14341 gcctgatgcc tgaacttgcc gaaagctggg aagtactcga caacggcgcg acctatcgct
    14401 tccacctgcg tcgcgatgtt ccgtttcaaa aaaccgactg gtttactccc actcgtaaaa
    14461 tgaatgccga cgatgtggtg tttaccttcc agcgaatttt tgaccgcaac aacccgtggc
    14521 ataacgtcaa cggcagcaac ttcccctact tcgacagcct gcaatttgcc gataacgtga
    14581 aaagcgtccg caaactggat aatcataccg ttgagttccg actcgcccag ccggatgctt
    14641 cttttttgtg gcacctcgca acccattatg cttcggtcat gtcggcagaa tatgcccgga
    14701 agttagagaa agaagatcgc caggagcaac tcgaccgtca accggtcggc accggtccgt
    14761 atcagttgtc ggaataccgc gccgggcaat ttattcgcct acaacgtcat gatgacttct
    14821 ggcgcggtaa accgttaatg ccgcaggtag tggtggattt aggctccggc ggcaccggac
    14881 gtctgtcgaa actcctgacc ggggaatgcg acgttctggc ctggcctgct gccagccagc
    14941 tatccatttt gcgtgacgac ccgcgcttgc gtttaacgct gcgtcctggg atgaacgtcg
    15001 cctatctggc atttaacacc gccaaaccgc cgctaaataa tcccgctgtc cgccatgcac
    15061 tggcactggc gattaataac cagcgcctga tgcaatccat ctattatggt acggctgaaa
    15121 cggcggcctc tattttaccg cgcgcctcgt gggcctatga caacgaggct aaaattactg
    15181 aatacaatcc ggcgaaatcg cgcgaacagt tgaaggcgtt ggggctggaa aacttaacgc
    15241 tgaaactgtg ggtgcccaca cgttcgcagg cgtggaaccc cagtccactg aaaactgccg
    15301 aactgattca ggcggatatg gcgcaggttg gcgtaaaagt ggtgattgtg ccggtagaag
    15361 gtcgctttca ggaggcgcgg ttgatggata tgagccatga tctgacgtta tccggttggg
    15421 cgacggacag taacgacccg gacagtttct tccgtccgtt actgagctgc gcggcaattc
    15481 attcacagac caacctcgcc cactggtgcg atccgaaatt cgacagcgtg ttgcgtaagg
    15541 cgctctcctc gcagcagctg gcagcgcgta ttgaagccta tgacgaagcg cagagtattc
    15601 tggcgcagga attacctatt ctgccgctgg cgtcgtcatt gcgtttgcag gcctatcggt
    15661 acgatatcaa aggtctggta cttagcccgt ttggtaacgc ctcctttgct ggggtgtatc
    15721 gcgagaaaca ggatgaggtg aaaaaaccat gattatcttc accttacgcc gcattttgtt
    15781 attgattgtc accttgttcc tgctgacctt cgttggcttc agcttgagct atttcactcc
    15841 acacgcgccg ttacaaggcg cgtcactgtg gaatgcctgg gtgttctggt ttaatggcct
    15901 gatccactgg gattttggtg tttccagcat taacggtcaa ccaattgccg aacagttaaa
    15961 agagctcttc ccggcgacga tagaactgtg catcctcgcc ttcggttttg cgctgattgt
    16021 tggcatcccc gtggggatga ttgccgggat tacgcgccat aagtggcagg ataacttgat
    16081 caacgccatc gccttactgg gcttttcaat cccggtgttc tggctggcgc ttctgttgac
    16141 actgttttgt tcactcacgc taggttggtt gcccgtttca gggcgtttcg atctgctcta
    16201 cgaagtgaaa ccgattaccg gttttgcgtt gattgatgcc tggctttcgg actcaccatg
    16261 gcgggatgaa atgatcatga gcgcaatccg ccatatgata ttgcccgtga tcaccctgtc
    16321 ggttgcgcca acaactgaag tgatccgcct gatgcgtatc agcactattg aagtgtatga
    16381 ccagaactat gtaaaagcgg cggcgacccg cggtttgtcg cgctttacta ttttgcgtcg
    16441 ccacgttctg cataacgcgt taccgccggt tattcctcgc ctgggcttac agttttccac
    16501 catgctgacg ctggcgatga tcaccgaaat ggtttttagc tggccgggtt tagggcgctg
    16561 gttaattaac gccatccgcc agcaggatta cgcagccatt tccgccggag tgatggtgtg
    16621 tggctcactg gtcattattg ttaacgtgat ttctgatatt ttgggtgcca tggctaaccc
    16681 tctgaaacat aaggaatggt atgccttacg atagcgtata cagcgaaaag cgcccgccgg
    16741 gcacgctgcg taccgcctgg cgcaaatttt atagtgacgc ctctgcgatg gtcggcctgt
    16801 acggctgcgc gggactggct gtactgtgta tttttggcgg ctggtttgcg ccttacggca
    16861 tcgaccagca atttctcggt tatcaattat tgccgccatc atggtcgcgc tatggcgaag
    16921 tttctttctt cctggggact gacgacctcg gacgtgatgt tttaagccgt ttactgagcg
    16981 gagctgcgcc aaccgtgggt ggcgcatttg tggtgacgct tgccgcgacg atctgtggcc
    17041 tggtactggg caccttcgcc ggggcgacac acggcctccg ctcagcggtg cttaaccata
    17101 ttctggatac cttactggcg atcccttcgc tgctgttggc aattatcgtt gtagcgtttg
    17161 ccggaccgag tttgtcccac gctatgtttg ctgtctggct ggcattgctg ccgcgtatgg
    17221 tacgttcgat ttacagcatg gtgcatgacg aactggaaaa agagtacgtt atcgccgccc
    17281 gtctggatgg cgcatcaacg ctgaatattc tctggtttgc tgtgatgcca aacatcaccg
    17341 ccgggctggt caccgagatc acccgcgcac tgtcgatggc aattctcgat atcgccgcgc
    17401 tgggctttct cgatctcggc gcacaactcc cctcgcctga atggggagcg atgctcggtg
    17461 atgcgctgga actgatttat gtcgccccgt ggactgtcat gctgccaggt gcggcaatta
    17521 tgattagcgt gttgttggtt aacctccttg gcgatggcgt tcgtcgtgcg attattgcgg
    17581 gggtggaata atgccattac tcgatattcg taacctgacc attgaattta aaaccggtga
    17641 tgagtgggtt aaagccgtcg accgggtaag tatgacctta accgaaggtg aaatccgcgg
    17701 tcttgttggt gaatccggtt ccggcaagag tttgattgcg aaagcgattt gtggggtgaa
    17761 taaagataac tggcgtgtta ctgctgaccg tatgcgtttt gatgatatcg atttgctgcg
    17821 tctctccgca cgcgaacggc gcaaactggt tggccataac gtgtcgatga ttttccagga
    17881 accgcagtcg tgtcttgacc cttcagaacg tgtgggtcgc cagttgatgc aaaacatccc
    17941 agcctggacc tataaaggcc gttggtggca gcgttttggc tggcgcaaac gccgtgccat
    18001 tgaactgctg caccgcgtgg ggattaaaga tcacaaagat gcgatgcgca gttttcccta
    18061 tgagttgacc gaaggtgaat gtcagaaagt gatgatagcc attgcactgg cgaatcaacc
    18121 gcgtctgctg attgctgacg aaccgacaaa ctcaatggag ccaacaaccc aggcgcaaat
    18181 ctttcgcctg ctgacgcgtc tcaaccaaaa cagcaatacc actattttgc ttatcagcca
    18241 tgacttacaa atgcttagcc aatgggcgga taaaattaac gtgctttact gcggtcaaac
    18301 agtggaaacc gcgccgagta aggagttggt gacgatgcca catcatcctt atacccaggc
    18361 gctgatccgc gcgataccag acttcggcag cgcgatgccg cataaaagtc gcctcaatac
    18421 tctacccggc gctatcccat tgctggaaca gttaccgatt ggttgtcgtc tggggccacg
    18481 ttgcccgtat gcacaacgag aatgcattgt gacgccacgt ttgacggggg cgaaaaatca
    18541 tctctatgcc tgtcatttcc cgctgaacat ggagaaaaag tgagatgatc gaaacgctgc
    18601 ttgaagtgcg taatttaagt aaaacgttcc gctaccggac cggttggttt cgtcgtcaga
    18661 ccgtagaagc ggtaaaaccc ttgagcttta cgctacgtga aggccagaca ctggcgatta
    18721 ttggcgagaa tggttcgggt aaatccacac tggcaaaaat gctggcggga atgatagaac
    18781 ctaccagcgg cgagttattg attgacgatc atccgctgca ttttggcgat tattccttcc
    18841 gtagtcagcg tattcgcatg atttttcagg atccttcgac ctcgttgaat ccccgtcaac
    18901 gtatctcaca aattctggat tttccactgc gcctgaacac cgacctggaa ccggaacagc
    18961 gtcgcaaaca gattattgaa acgatgcgta tggttgggct gctaccggat cacgtcagtt
    19021 actatccgca tatgttggca cccggacaaa aacagcgtct gggtctggcg cgcgcgctga
    19081 tattgcgccc aaaagtcatt attgccgatg aagccctcgc ttcactggat atgtcaatgc
    19141 gttcgcagtt gattaatctg atgctggagt tacaggaaaa acagggcatt tcgtatattt
    19201 atgttaccca gcatatcgga atgatgaagc acattagcga ccaggtgctg gtgatgcatc
    19261 agggcgaggt tgtcgagcga ggcagcaccg cggatgtgct ggcatcgccg ctgcatgaac
    19321 tcaccaaacg gctgattgcc gggcactttg gcgaagcatt gacagcggat gcgtggcgta
    19381 aagatcgcta attttctgaa tcctccttcc agattattca cctcattctg acaagagatt
    19441 aaatcttgtc agaatgaggt gatggataaa ataaaatcaa atgcgcgtga tttaagacgc
    19501 aatctcactt tgcaggaacg aaagctctgg cgatatcttc gcagccgacg ttttggtgat
    19561 ttcaaatttc gccgtcaaca tccggtgggg agctacattc tcgattttgc ttgctgctcg
    19621 gcgcgcgtag tcgttgagct ggatggtggg cagcatgatt tggcagttgc ctatgatacc
    19681 aggcgcacta gctggcttga gtcgcagggc tggaccgtgc tgcgtttctg gaataacgag
    19741 attgattgta atgaggaggc ggtgctggag attattctgc aggaactgaa ccgccggtca
    19801 ccctcaccct gaaagggcga gggggccgac cgagccgaat agctgttgtg gtgaaaacat
    19861 ggagacagtg ctggagaata ttcggcaagg tctgaaccat cccagccatc gccataaaag
    19921 ggttaggggc tgtatgagcc tgtttgttgc tggggtaaca atatttgcac aatatggtcc
    19981 cctcgcccct ctggggagag ggttagggta aggggaaaag cgccccccct gccgcagcct
    20041 gctccggtcg gacctggcaa ctatagctac tcacagccag gttgattata ataaccgttt
    20101 atctgttcgt actgtttact aaaacgacga atcgcctgat tttcaggcac aacaagcatc
    20161 aacaataagg attaaagcta tgggttttct ttccggtaag cgcattctgg taaccggtgt
    20221 tgccagcaaa ctgtccatcg cctacggtat cgctcaggcg atgcaccgcg aaggagctga
    20281 actggcattc acctaccaga acgacaaact gaaaggccgc gtagaagaat ttgccgctca
    20341 attgggttct gacatcgttc tgcagtgcga tgttgcagaa gatgccagca tcgacaccat
    20401 gttcgctgaa ctggggaaag tttggccgaa atttgacggt ttcgtacact ctattggttt
    20461 tgcacctggc gatcagctgg atggtgacta tgttaacgcc gttacccgtg aaggcttcaa
    20521 aattgcccac gacatcagct cctacagctt cgttgcaatg gcaaaagctt gccgctccat
    20581 gctgaatccg ggttctgccc tgctgaccct ttcctacctt ggcgctgagc gcgctatccc
    20641 gaactacaac gttatgggtc tggcaaaagc gtctctggaa gcgaacgtgc gctatatggc
    20701 gaacgcgatg ggtccggaag gtgtgcgtgt taacgccatc tctgctggtc cgatccgtac
    20761 tctggcggcc tccggtatca aagacttccg caaaatgctg gctcattgcg aagccgttac
    20821 cccgattcgc cgtacagtta ctattgaaga tgtgggtaac tctgcggcat tcctgtgctc
    20881 cgatctctct gccggtatct ccggtgaagt agtccacgtt gacggcggtt tcagcatcgc
    20941 tgcaatgaac gaactcgaac tgaaataatc gttctgttgg taaagatggg cggcgttctg
    21001 ccgcccgtta tctctgttat acctttctga tatttgttat cgccgatccg tctttctccc
    21061 cttcccgcct tgcgtcagga taacgatttc ctttacgacc aaggagcgcc catggaacaa
    21121 cgccacatca ccggcaaaag ccactggtat catgaaacgc aatccagtac tacggagtat
    21181 gacgttctgc ctctggtccc ggaagccgca aaggtcagcg atccctttct gctcgacgtg
    21241 atccttgatg aagagacgct ggcccccttc ctttcatggc tggtccctgc gcgcgttctt
    21301 gcagtggagt tgttccctga ccagcttacc gtgacccgtt cacaaacctt caccgcttat
    21361 gaacgcttgt cgacggccct gacggttgct caggtttgcg gcgtccagcg gttatgtaac
    21421 tactattcgg cgcgacttac gccgctcccc ggacctgatt cctccaggga aagtaatcat
    21481 cggttggcac aaatcacgca atatgcccgc caactggcta gctcgccttc tattatcgac
    21541 aaccgatcgc gccagcatct gaatgacgtc ggtcttactg tctgggactg tgtgatcatt
    21601 aaccaaatca ttggttttat tggctttcag gcgcggacaa ttgcgacatt tcaggcttat
    21661 ctcgggcatc cggtacgctg gttacccggg ctggcgatac aaaactacgc cgacgcgtca
    21721 ctgtttgctg atgaatcaat acgctggcga agcagctatg aggtggaaaa actacctgaa
    21781 gagcacacaa aaagttcaac tgcagaactt tgccaactgg ccgaaacaca ctctctccac
    21841 cctatttcac tttcccttct cgaaaagttg ttaaacagca cacgggtcaa tgcacagccg
    21901 gataatcagc ttgcggcgtt gttatgcgcg cgtataaatg gcagtcctgc ttgtttttcc
    21961 acctgtatgg attcatcaaa tgaatataaa aaaatcagca cccttctgcg caagggcgaa
    22021 aatgaaatta accgatgggc tgaccgtcat tctgttgagc gcgctaccgt tcaggcgata
    22081 caatggctga cccgagcacc cgatcgcttt agcgccgccc agttcagccc tttactcgaa
    22141 cacgaaaaat catcaacgca gattattaat ctgctggtat ggagcgggct gtgtggctgg
    22201 ataaatcgct taaaaatcgc gttgggtgag acatattaac cttgccgcgt cagacagatt
    22261 cgcgtaaaac tgtcagccgc tctaatggcc accaaaatag acaattatgt ttcaggacaa
    22321 cccgctgcta gcgcagctta aacagcaact gcattcccag acgccacgcg ctgaaggggt
    22381 ggtaaaagcc acagaaaaag gctttggctt cctggaagtc gacgcacaaa aaagttattt
    22441 cattccgccg ccgcagatga aaaaagtcat gcatggcgac cgaattatcg cggtgatcca
    22501 cagtgaaaaa gaacgtgaat ccgcagagcc agaagaactg gttgaaccgt tcctgactcg
    22561 tttcgtgggt aaagttcagg gcaaaaatga ccgtctgacc atcgttcctg atcatccact
    22621 tttaaaagac gccattcctt gccgcgcagc ccgtggcctg aaccacgagt ttaaagaagg
    22681 cgactgggcg gttgccgaaa tgcgccgtca tccgctgaaa ggcgatcgtt ctttctatgc
    22741 tgaactgaca caatacatca cttttggtga cgaccacttt gtaccgtggt gggttaccct
    22801 tgcgcgccat aatctggaaa aagaagcacc agacggcgtc gctaccgaaa tgctcgatga
    22861 aggtctggtt cgtgaagatc tgacctcgct ggattttgtc accatcgaca gtgccagcac
    22921 tgaagatatg gatgacgccc ttttcgctaa ggcgctgccg gatgacaaac ttcagctgat
    22981 tgtggcgatt gccgatccaa ccgcgtggat tgctgaaggc agcaagctgg acaaagccgc
    23041 gaaaattcgc gcattcacca actatctgcc tggcttcaac atccctatgc tgcctcgcga
    23101 gctttctgac gatctctgct cactgcgcgc caatgaagtc cgcccggtac tggcatgccg
    23161 catgacgctc tccgctgatg gcaccactga agataatatc gaattctttg ccgccaccat
    23221 cgaatccaaa gcgaagctgg tgtatgacca ggtttctgac tggctggaaa ataccggtga
    23281 ctggaagcct gaaagtgaag caattgccga acaagtccgt ttgctagcgc aaatttgcca
    23341 acgccgcggc gagtggcgtc ataaccacgc actggtgttt aaagatcgcc cggattaccg
    23401 ctttattctc ggtgaaaaag gtgaagtgct ggatatcgtc gccgagcctc gtcgcattgc
    23461 caaccgtatc gtcgaagaag cgatgattgc cgctaacatt tgtgcggccc gcgtactgcg
    23521 cgataagctc ggttttggca tctataacgt gcatatgggc tttgatccgg cgaatgccga
    23581 cgcgctggca gcgttgctga aaacgcacgg tctgcatgtc gatgccgaag aagtgctcac
    23641 gctggacggt ttctgcaaac tgcgtcgtga actggatgcg caaccaactg gtttcctcga
    23701 cagccgcatt cgtcgcttcc agtcatttgc tgaaattagc tctgaacccg gtcctcactt
    23761 tggcctcggt ctggaagcat acgccacctg gacttcgccg atccgtaaat atggcgacat
    23821 gatcaaccac cgtctgctga aagcggttat caaaggcgaa actgcgacgc gtccgcagga
    23881 tgaaatcact gtccaaatgg ccgaacgtcg ccgtctcaac cggatggcag aacgtgatgt
    23941 tggcgactgg ttatacgcac gcttcctgaa agataaagcc gggaccggca cccgtttcgc
    24001 ggcggaaatt gtcgatatca gccgtggcgg catgcgtgtg cgtttggttg ataacggcgc
    24061 tatcgccttt attccggcac ctttcttaca cgctgtgcgc gatgaactgg tttgcagcca
    24121 ggaaaacggc actgtacaaa ttaaaggtga aacggtttac aaagtaactg acgttattga
    24181 cgtcaccatt gccgaagtcc gcatggaaac ccgcagcatt attgcgcgcc cggtcgcgta
    24241 atctcctttc acggcccatt cctcatggat gggccgttta tttccccgct ctaccttcgt
    24301 tatatccagg cagatttttt aactgacttt cgtttgaaaa ctggcgtttt tccaaaactg
    24361 gattacaaat aaatatataa aaaacaatgc gtttagccgc cgttaatttc atgtaagtca
    24421 tgttgttcct tttatttaac cgcggactcc gctgttaacc ggaggatatg catcatgaaa
    24481 accgttaggg agtccacaac gttgtacaac tttctcggat cgcacaatcc atactggcgg
    24541 ttgacggaaa gcagcgatgc tttgcgcttt tctaccacca aaaccacaga acctgatcgt
    24601 acattgcagt tatctgccga acaggctgct cgcatcaggg aaatgacggt catcacctcc
    24661 agcctgatga tgagtctgac cgtcgatgaa agcgatcttt ctgtgcatct ggtaggacga
    24721 aaaatcaata aacgggaatg ggctggcaac gcgtctgcat ggcatgacac accggcggtt
    24781 gctcgtgatt tatcacacgg gctttccttt gctgagcagg tagtttctga agcacattcc
    24841 gcaatagtga ttctcgacag ccgggggaat atccaacgct tcaatcggtt atgtgaagat
    24901 tacacagggt tgaaagaaca cgacgtcatt gggtaaagcg tgtttaaact gtttatgagc
    24961 cgtcgtgaag ctgcggcatc caggcgcaat aaccgtgtat tttttcgaag cggcaatgca
    25021 tatgaagtcg aactgtggat accaacacgt aaaggccagc ggctgtttct gtttcgcaat
    25081 aaatttgtcc acagcggcag tggcaaaaac gagatttttt taatctgttc cggtaccgac
    25141 attaccgaag agcgccgcac tcaggagcga ctgcgtattc tggcaaatac cgacagtatc
    25201 accggactgc cgaatcgtaa cgcaatgcag gagttaatcg atcacgctat taatcaggca
    25261 gataacaata aagttggggt tgtgtatctt gatttggata attttaaaaa ggtcaacgac
    25321 gcctatgggc atttgtttgg tgaccagtta ttacgcgacg tgtcattggc tattttaagc
    25381 tgtctcgaac atgaccaggt gttggcgcgt ccaggtggcg atgagtttct ggtactggca
    25441 tccaatacct cacaaagcgc gctggaagca atggcatcac gaattttgac ccgcttacgc
    25501 ctcccctttc gtattggttt aattgaagtt tataccagct gttcagtagg tatttcgctc
    25561 tctcccgaac atggttcgga cagcgcgact attattcgtc acgccgacac agcaatgtac
    25621 acagcgaagg aaggcggacg aggacaattt tgcgttttta ctccagaaat gaatcagcgg
    25681 gtatttgaat atctctggct ggataccaac ttgcgtaaag cactggaaaa cgatcagttg
    25741 gttatcgaaa atcacctagc gtggcgaagt gcgcagtctg gaagcactag tacgttggca
    25801 gtcacctgaa cgtgggttga ttccaccgtt ggacttcatt tcctacgccg aagagtccgg
    25861 gctaattgtg cctttaggcc gttgggtgat tctcgatgtc gtacgccagg tggcaaagtg
    25921 gcgggataaa ggcataaacc tgcgagtggc ggtaaatatt tctgcacgtc agctcgccga
    25981 tcaaaccatt ttcaccgccc tgaaacaggt tctccaggaa ctcaattttg aatactgccc
    26041 tatagatgtt gaactgacag agagttgtct gattgagaat gatgaactgg cactgtctgt
    26101 tattcaacaa tttagccaac taggtgcgca agtgcatctg gatgattttg gtaccggcta
    26161 ctcttcactt tcgcaactgg cgcgctttcc gatcgatgcc atcaaacttg accaggtttt
    26221 tgttcgagat attcacaaac aacctgtctc gcagtcactg gtccgggcga tcgtcgctgt
    26281 ggcccaggca ttgaatcttc aggtgatcgc cgaaggtgta gagagtgcaa aggaagatgc
    26341 ttttttaacc aagaacggga tcaatgagcg gcaaggattt ttgtttgcca aaccgatgcc
    26401 cgccgtcgcc ttcgaacgct ggtataaacg ctatctgaag cgcgcataag tagatgaata
    26461 tatatactta gcgctatatc ccgtcattat atatcgtatt cctggatgat aattagccgt
    26521 tgctgttaaa cacttctctt ttagcggcaa tttttgcatt attcattttc ccccttttgc
    26581 tataaggatg tccttgatgt ctgaatttga tgcccagcgc gttgccgaac gtattgatat
    26641 tgtgttagat atccttgtgg caggcgatta ccactctgcg atccataatc tcgagatact
    26701 taaagcagaa ctcttgcgcc aggttgctga atctacgcca gacatcccga agacaccgtg
    26761 ggagatttaa caatcgtgcc ttcaggtttt cctatttgag gatgcccggc gcgtcagtat
    26821 ttttttattt agtattataa cgttataaga attacagcga tgaattcccg acaacaaact
    26881 attctacaga tggtcattga ccagggtcag gttagcgtaa ccgatctggc aaaagccact
    26941 ggagtttctg aagttaccat tcgccaggat ctcaacaccc tcgaaaaact gagttacctc
    27001 cgccgtgcac atggctttgc agtttcgctt gatagtgatg acgtcgaaac ccgtatgatg
    27061 agcaactata cgctgaagcg tgaactcgcc gagtttgccg cgtcactggt tcaaccgggc
    27121 gaaaccatct ttatcgaaaa tggcagcagc aatgccctgc tcgcccggac tctgggggag
    27181 cagaagaaaa atgtcactat catcacggtc agcagctaca tcgcgcattt gctgaaagac
    27241 gcgccttgtg aagtcatttt gctcggtggc gtgtaccaga aaaaaagcga aagtatggtt
    27301 ggccctttga cacgccagtg catccagcag gtacatttca gcaaagcatt tattggtatt
    27361 gatggctggc aacctgaaac tggattcacc ggtcgcgata tgatgcgcac cgatgtggtc
    27421 aatgccgtgc tggaaaaaga gtgcgaagcg atagtcctga ctgacagctc gaaatttggt
    27481 gctgtacatt catactccat cggtcccgtt gagcgattca atcgcgtgat taccgattcg
    27541 aaaatacgcg ccagcgatct gatgcatctt gagcacagta aactcaccgt tcacgtcgtt
    27601 gacatttaac catttccctt ctctacggat gatttgcagt ttggcaaatc atccgctcta
    27661 agatgattcc tggttgataa ttaagactat ttacctgtta ttaacactct caagatataa
    27721 aattattatc agcgatataa caggaagtaa ttatcacctg cgtgatataa ccctgcgcgc
    27781 gagcagattt cacggaataa tttcaccagg cttattctta gctattatag ttatagagag
    27841 cttacttccg tgaatcataa attcaggaga gagtattatg tttgtaacga gcaaaaaaat
    27901 gaccgcggct gttctggcaa ttactttggc aatgtctctg agtgcctgtt ctaactggtc
    27961 taaacgggac cgcaacaccg caatcggcgc gggtgcaggg gcattaggcg gtgcagtact
    28021 gaccgatggc agtacgttgg gtacattagg tggtgcagct gtcggtggtg ttattggtca
    28081 ccaggtcggt aaataatcgt aaataaatgt cgattatcga ctcgcaatta taatacgctt
    28141 gattctgttc agtacgaaat aactgaataa tacacacagt aaaatattcg aaagccacgg
    28201 atatatccgt ggctttttat gttaaccgcc tgcgagtttt accttcatcc ctttcgcttc
    28261 aagtagtgat tttaataaat cacgcttatc gccctggatt tcaataactc catctttaac
    28321 tgctccgccg cagccgcatt ttttcttcag ttccgctgca agttttgtca gttcggcatc
    28381 atcgagatcg ataccggtaa tcaggcaaac gcccttacct ttacgtccac tggtctgacg
    28441 ctgaatacgc accgctccat cgcctttagg gcgtactggg gccgctttgg gctcatcaat
    28501 acgcccggtt tccgttgagt agaccagacg gctgttggaa tcactcatca tgcactccgc
    28561 tgtaaagagg cgttgatcgc tttcagtgtc tgcgctggat ctaccgattg cgttaccggg
    28621 cgaccaatca ccatataatc aacaccagcc gccaacgcct gttctggcgt cataatgcgg
    28681 cgctggtcac cagcttcact cccctgcgga cgaatgcccg gtgtaaccag tttgaactcc
    28741 tgaccgaata cctgtttaaa gcgcacagct tcctgagcag aacacaccac accatcaagg
    28801 tcacattttt gcgtcagtgc tgccagacgt tctgcataat ctgcaggtga cagtgtcacg
    28861 ccaagatcgg ccaggtcgct ggcttccatg ctggtcaaca ctgtcacagc aatcaaaagc
    28921 ggtgcatctt tgccaaacgg aaccagtgcc tcacgcgctg cggtcatcat acgcgcccca
    28981 ccggaggcat gaacattcac catccacacg cctaagtcag ctgcagcagc gacagcgtgc
    29041 gctgcagtgt tggggatatc gtggaatttc aggtcaagaa agatatcaaa accacgctgt
    29101 tgaagttcgc gcacaaactg tggcccaaac aatgtaaaca tctctttacc gaccttcaga
    29161 cgacaatcgc gtgggtcgat cttgtcgaca aaggacagcg cgtcatcaca attatgataa
    29221 tcaagggcaa caaccacagg agaattcgta acggcgcggg aagaagatga agcagttaac
    29281 gtcatgacca gaccttcttg atgatgggca ccgggagtgg cgcgaaacag gtaaacggcg
    29341 agcattctac ccgtgaaaat aaaaaattaa cagttgcgat ttttccccta gcaggctttt
    29401 tctgctccgt cgttgccgga gcacaatggg acatcaataa ctagtatgtt gtaactaaag
    29461 tcggattttt ttaaaaatta caggccatca agaccgcgaa tcggtttaat agttgaccag
    29521 gcacgacaag acggacaatg ccagtagagg gtgtatgcag taaaaccaca tttctggcag
    29581 ctataacgag gcttactacg caccttctcg ccaaccatgt cacgcagcac catcagactc
    29641 tctttggcgc gcccttcttc cgcttcattt aagtggtaat ccattagctt atggaataca
    29701 cgcatggtcg gatgacgctg aagctggcgt gtaatataga cctgtgcagc ctcactaccg
    29761 tcgcgcgctt cgatgatatc agcaagcatc aattcagcat cggcaccggt gttctcttcc
    29821 accgcgcgtt gcaggaattc tgcccattcg gcagttttac ccaactgctg atagcaggtt
    29881 tgcaacattt ccagcgtttc gctgaccagt tctctgtcct gggatatgac gcgttgcaaa
    29941 ctttcgacgg ctttggcgta ttctcctttc gccataaaca cgcgtcccat catgatggat
    30001 acgcgggcgc tgtttttatc tgccgccgcc ccttttttta gcaaggtcat ggcacgatcg
    30061 agatcgtcgc tggccatatg ctgcagggct aactcacagt agaaatgggc aatttcgacg
    30121 cgctgtttat ctttacccag cttcaccagg cgttcggcaa catcaattgc tttctgccac
    30181 tcactggtag cctggtagat ttgtagcaac tgttgcagcg cgccaatgcg gaagtcagtt
    30241 tcatcggtca gctgattgaa catgtcttcc gcgcggtcgt ataacccggc ggccatgtaa
    30301 tcacgcccca gttgttgaat cgccaacaga cgctgttcat aggtcagcga ggcgctttcc
    30361 attagggtct gatggatgcg aatagcgcga tcaacttcgc cacgcgaacg gaacaggttt
    30421 ccgagcgtaa ggtgggcttc aacggtgcct gtatcctctt taagcatatc gagaaacagg
    30481 tctaccgctt tatcctgttg attactaagc aggaagttaa cccccgctac gtaatcacgc
    30541 gacaagcggt tggcttcatc ttgcttgttt tgttgcgcac ttctgcggcc catataccag
    30601 ccataggcag cggctacagg caaaagcaga aacaacaact ccagcataga aagttattcc
    30661 ttcgccgctg acgagtgcgg cactacagcc acgtcagtcg cgggtgaaag ctggttttcc
    30721 agtcgcttta ttttacgttc agcgcgcgcc agggaaacac gaactcgcag ccagaacagg
    30781 ccacaaatca accaaccgat agcaaacccc gcagcaaaca ataccgccag caatgtggaa
    30841 atacggtact ccccttgcgc taacagataa ttaaacgtca cctgttgatc gttctgcgca
    30901 cccaacgtga ccgaaatcac gaagatcgct aacaccagta agaaaatgag taaatatttc
    30961 acattacttc ccgttatgtg gttagagcga ataaattatg gtcaacttac cgcaatagcc
    31021 ctttaaagta ccattttcgc gccgtctgcg aaatggcatc cacgcgcatt gttgatgatt
    31081 taaggcctaa attcggaaaa tcaaccagcg ttaactttct tgttctcgtt gtgcaatttc
    31141 gcgattttct tccgcaggtg gtgttaatgg cccacaaatt cgttgcgcaa gccacgttgc
    31201 caccgccacc agcgcccacg aaatcaacgt agctaccacc agatcgcgtg gccaatgcat
    31261 cccgagcagc aggcggcttc ccatgactcc cgttgcccag accaacaaga tagcaatggt
    31321 taacgttcgc cgacgcggcc acagcaaacc aacggccagc agtgcccaac tggcagcaaa
    31381 catcgtgtga ccggaaggaa aggcaaaccc cgtctctttc tgccagtgtg aacgcaaata
    31441 ttgtggaata tttttctctt cagccaactg ttcttttact agatttccgc gttctgctcg
    31501 ctttaaagtg tagaactcat caaccggaat atgatgtgtt ttttccaacc agataacaaa
    31561 aggtcgtggt tcctgtactt tgtctttgat ccaggattta acgccttgtc caacaaggat
    31621 tgccgccgcc agaatggcaa ataatacaaa ggcagcctta atgcgaaaac gcagacacca
    31681 gagaaaccag ccgaacaaaa tcaaatgtgt aatgacgccc cagggctggg tgacagtttc
    31741 agtaacccaa aaagccgctt ttagtagcca actttgttct ccaggttgcc aacgccagcc
    31801 agaaatccat acggctactg gcatgacaag caatagtgca gctcccactg cggtacgtcg
    31861 ggcaatcgaa cgcatggcct ctcctttttg ataagtccca caatcataac tgaaaatgcc
    31921 agttccaaga aaaattgaca gatttgtgcc attccgtgaa cgatcgacgc gtcgtgatta
    31981 ggtgaacccc ttctcgttat ggcaaaataa gccaatacag aaccagcatt atctggagaa
    32041 tttcatgcag cttaaacgtg tggcagaagc caaactgcca accccatggg gcgatttcct
    32101 gatggtggga tttgaagaac tggcaaccgg acacgatcat gtcgcgctag tctatggcga
    32161 tatttccggg cataccccgg tacttgcgcg cgtccattcc gaatgtctga ccggtgacgc
    32221 cctgttcagc ttgcgctgcg attgtggctt ccagctcgaa gcggcattga cgcaaattgc
    32281 cgaggaaggc cgtggtattt tgctgtatca ccgtcaggaa ggtcgtaaca ttggtctgct
    32341 gaataaaatc cgcgcttacg cactgcagga tcaaggttac gataccgtag aggctaacca
    32401 ccagttaggc ttcgccgctg atgagcgcga cttcactctt tgcgctgata tgttcaaact
    32461 ccttggcgtc aatgaagtcc gcttgttaac caataacccg aaaaaagtcg aaattctgac
    32521 cgaagcaggg attaatattg ttgaacgcgt accattgatt gtaggtcgta accccaataa
    32581 cgaacattat ctcgatacca aagccgagaa aatgggccat ttgctgaaca aataaccctc
    32641 ttgcatcgtg taattcattt gcttgccgga agcaaaataa ccggcaagca aatacttgtt
    32701 acttcaacat attacgaatg acataatgca aaatgccgtc gttctggtag taggtcaact
    32761 ccgtcgcggt gtcgatacga caacggcagg gtacgacttc ctggctacca tccgcgcgcg
    32821 taagcgtcac cggaaccgtc gcgccaggtt gtagattttg cagatcgcca atatcaatct
    32881 tctcttcccc ggttagccct aacgttttac gcgtcacgcc ttgcggaaat tccagcggca
    32941 ggatgcccat gccaattaaa ttcgaacggt gaattcgttc aaacgattcg gcaatcacca
    33001 cacgaattcc aagcagacga ggtccttttg ccgcccagtc acggcttgag cctgatccat
    33061 actctttccc ggcaatcacc gccagcggcg tttgctcctg cttatagcgc atcgcagcat
    33121 cataaataga gactacgtcg ctgtcaggta aatgccgcgt catcccccct tcaacgccag
    33181 gcaccatttc attacggatg cgaatattgg cgaaggtgcc gcgcatcatc acttcatggt
    33241 taccacgccg cgaaccgtag gagttaaagt cttttcgctc aacaccccga ccttgtagat
    33301 atcgacccgc tgggctgtcg ggcttaatac tgcccgccgg agagatatgg tcagtggtga
    33361 ctgaatcccc cagcattgcg aggatccgcg caccgtgaat atcttccact ggtgctggtg
    33421 ttgcctgcat ttcatcaaag aaaggcgata agcgaatata ggttgagtcc tcctgccaac
    33481 cgtaggtatc ggatcgtgtg acgttaattt ccttccactc tgctgtgcct tcaaaaactt
    33541 ctgcgtactc tttgcggaac atttctgtgg agacttgttc taccgcacgg gcaatttctt
    33601 gtgccgatgg ccagatatct ttcagataaa ccggctcgcc tttgcgatca tggccgatag
    33661 gctcagaagc caggttgata ttcatatttc ccgccagcgc ataggcaacc accagcggtg
    33721 gcgaggccag ccagttagtt ttaaccagcg gatggatacg gccttcaaag ttacggttgc
    33781 cggacagcac cgcaccgacg gttaaatcgc cttttttgat tgccgtttcg ataggatcgg
    33841 gcagcggccc agagttacca atacaggtgg tacaaccgta tcccacaagg ttaaacccca
    33901 gttcgtcgag atacggtgtc agtttcgctt ttgccagata atcagaaacg actttcgaac
    33961 ccggtgccaa cgacgctttg acccacggtt gccgcttgag gccaagagtt acggcttttt
    34021 tcgccagcaa gcctgcggcc atcagcacac ttgggttaga ggtgttggtg cacgaggtta
    34081 tcgcagcaat gaccacagcg ccatcaggta actgatactg atgtccgttc ataacataat
    34141 cgactggcag gcgatcttta tgcgtggcat tcacttccag ttcgttactg gcggcaaatg
    34201 cttttggtac atcgggcagt gcaacgcgat cctgtgggcg ttttggccct gccaggctcg
    34261 cttcaacgtc attcatatcc agttctaaca tactggtaaa aatcggttca tcgcccgggt
    34321 tacgccacat gccctgcgct ttggcatatt tttcgaccaa ctcgacctga tcttcgctgc
    34381 gcccgcttaa acgcatgtaa tcgagggtta ctgcatcgat tgggaagaag ccacaggtgg
    34441 caccatattc tggcgacata ttggcaatgg tggcgcgatc cgccaacggt agtgaatcca
    34501 gaccatcacc ataaaattcg acgaatttcc ccaccacgcc atgtttgcgc agcatttggg
    34561 taacagtgag aaccaggtct gtggcggtaa taccttcacg taattttccg gtaagtttaa
    34621 agcccactac gtccgggata agcatggaaa ccggctggcc taacatggcg gcttctgctt
    34681 cgatcccacc cacgccccac cccagcacgc caaggccgtt gatcatagtg gtgtgcgagt
    34741 cagtaccaac gagtgtatcc ggataagcaa tccattcacc gtcctgcaat tcgctccaca
    34801 ctgctttgcc gagatattcg aggttaacct gatggcaaat gcctgtgcct ggcggcacga
    34861 cgctaaaccg actgaacgct tgctttcccc atttcaggaa cacataacgt tcgtggttgc
    34921 gctccatttc caggcgtacg ttttcttcaa atgcctcatc atcaccaaaa cgatcgacgg
    34981 tcaccgagtg gtcaatgacc aggtcgaccg gtgagagcgg gttaaccttt gcagtatcgc
    35041 caccgaggcg tttaaccgct tcgcgcattg ccgccagatc aacaacggca ggtacgccgg
    35101 taaagtcctg catcagcacc cttgccgggc ggtaggcaat ttcacggtca gcatgggcat
    35161 ttttcagcca tcccgccagt gcgtggatat cctcttcggt aactgagtta ccatcctgcc
    35221 agcgcagcag gttttcgagc aaaactttga gtgacttggg tagacgggtg atatcgccca
    35281 gtgatttagc agcaagcggc aggctgtagt agtggtaagt tttatctttg gcctgcaacg
    35341 tgtccttact ggcttctcgt agggttgacg acatagctcc tccttaatga cagggttgcg
    35401 aagccctgat tctcttcagg gtgaatatta aagataacac aaacatcgcg taacgatttg
    35461 ataacaaccc aaattgataa aagaggagaa accttgtggc aaagcaaatg acaaccccgc
    35521 cgcagcgggg tcaaggatca gtgagtaatg atccagagga gttcggccca gaagagcatc
    35581 gacagagaaa acgcgcccat ccaggaccaa tatttcatat ttgtgttgat cataggctac
    35641 acccttaacg gttattattt tgtgttgaga taactcaagc tgttattgct gcggtgtatt
    35701 aaaagccctt taattcatta aaaatgggta ataagaatga tgataattat tgtttcggct
    35761 gatcatcttc agcaaacagt tctataaagg cttgctgctg cgatgttagt tgccagtcgt
    35821 ctggaataac gacggaacat tcccgtttcg tttctggttc attgtcacta tgctggcata
    35881 atggtgacga ctccagttgc tcctgaccga ccatagttat ccccacactt tccaaatcag
    35941 cagtgcgaca accacccaga aaagtgcaga accaagaaaa accgccagcc aggctttacg
    36001 ttttaattca ggatcacggc ggggttcctg atttccagaa ggcattgcta acctcatata
    36061 atcgccatca cttatcagca agacgttgaa tgattggtgt aattaatcat acgatgagac
    36121 aaatcctaaa gaaactttag tcaaaaatcc agcgaattta cgaatcgtct ccagtgaata
    36181 attcaatctt ttgacctgaa atagatattt gaaaactgga atttgcgagg cgggtaatta
    36241 gacactactt tacaggtaat ttaccctata aatatgaggg ataaaaatat tgtgcacggt
    36301 atagaaataa gtaacaaaag agaatatatt ccggcacctt cgctacataa aaggtgccga
    36361 aaataacgca agaaattatt tttccggcag ttttatatct ttaaacatga cctcaatttc
    36421 ttcattggag cgcaatgcga cagccgcatc aacgacatca cgcgttaaat gcggtgcaaa
    36481 acgctgaatg aaatcataca tataactgcg caaaaaagta ctacggcgaa aaccaatttt
    36541 ggttgtactg tggctgaaga tatcgtgagc atcaacgcgc acaaggtcgg gatcggcgac
    36601 cggatccacc gccatgctgg caatgacccc tacccccagc cctaaccgga cgtaagtttt
    36661 aatgacgtca gcatccgttg ccgtgaaaac gatacgcggc gttaaccctg cgcggttaaa
    36721 ggcagtatcc agttctgaac gtccggtaaa gccgaaggta tatgtcacca acggatattg
    36781 cgccagttct tcaatggtaa tggctttttt gcctgccagc gggtgatccg gagtgactac
    36841 aatagcccga ttccagtggt agcacggtaa catcactaaa tcttcataca gatgcagcgc
    36901 ttctgtggcg atagcgaaat cggcattgcc tttagagacg gcatcagcaa tttgtgtcgg
    36961 cgggccctgg tgcatatgca aagaaacgcg gggataacgc tcaataaagc ctttgataac
    37021 gtttggtaac gcgtagcgtg cctgggtatg cgtggtggcg atatacagcg agcctttatc
    37081 cggccaggtg tgctcaccgg caaccgattt tatggcatcg actttcgaca ggacttcgcg
    37141 agcgatacga attatttctt gtcctgctgg cgttacctgc gtcaggtgct tgccgcttcg
    37201 ggaaaaaatt tgaatgccta gctcgtcttc cagcattctg acttgtttac tgatcccggg
    37261 ttgtgatgtg taaagtcctt ccgctgttga tgagacattc aggttatgat tgaccacctc
    37321 aacaatatag cgaagttgtt gtaatttcat gttaaaccat ccacttagac gccatcagaa
    37381 catcgttttt tcgtcacacc tggcgggtga ctaatacgat ttaataataa aaaaggtgct
    37441 aactataacc actatatcat ttatagcctt agtgtatagg tgtgaagaaa aaataatatg
    37501 caataaaaaa gggccgcttt cgcgaccctt tgtttataaa aacctgacag aattaaaggt
    37561 tatttttttc cttcaaccca tttgccatca acataaaatg ctgaccagcc agtcgccttt
    37621 ccgtcttttt ccgaagagac atattgctgt ttggttttac ggctaaagcg aaccatggtc
    37681 ttattacctt ccggatcctg ctgtggcgca tcggccagat aacgcagttt ttccggcaga
    37741 cggtcgcgga agcgataaag ctcttccacc agtggcgcac gcgtttcacg cgatttcggg
    37801 aaagtgttgg cagccaggaa cacaccggca gcaccgtcac gcagcacgaa ataagtatct
    37861 gatttttcgc acggcagctc aggtaatggc accggatctt ctttcggtgg tgccacttcg
    37921 ccgttacgta aaatcttacg tgtgttttta cactcttcgt tggtgcaggc catgtattta
    37981 ccgaaacgcc ccattttcag gtgcatttca gagccacatt tttcacactc aacgatcggg
    38041 ccgtcataac ctttaatgcg gaattcgccc tcttcgatct cgtaaccgtc gcaggttggg
    38101 ttattaccac agacatgcaa cttacgtttc ggatcgatga gatagctgtc catcgccgtg
    38161 ccgcatttcg ggcaacgacg ttttgcgcgc agcgcgttgg tttcagcatc ttcgccttcc
    38221 agcacgttca gcacttcgtt ttccggcacc aggttaatgg tggttttgca acgctctttc
    38281 ggcggcagcg catagccaga acagccaagg aataccccgg tactcgctgt gcgaatcccc
    38341 attttgcgac cacaagtcgg gcagtcaatg ctggtcagaa ccatctggtt cgggcgcata
    38401 ccaccctctt ccggatcttt ttcagcttta tctaactgct gggtgaaatc cgagaagaag
    38461 tgatccagta cagctttcca ctctgcttcg tgatttgcca cctggtcgag gctgttttcc
    38521 atctgcgcgg taaagtcgta gttcattaac tcgcggaaat tttcttcaag gcgatcggtg
    38581 acgatttcgc ccattttttc cgcatagaaa cgacgatttt ctactcgcac atagccacga
    38641 tcctgaatgg tcgaaatgat cgacgcatag gtagacggac gaccgatacc acgtttttcc
    38701 agttctttaa ccagcgatgc ttcactgaaa cgggctggcg gcttggtaaa gtgctgggct
    38761 ggtgttagtt caacgagcgt cagagcatcg cctttatcga ctgccggtaa gatacgatct
    38821 tcatcgcctt tacgcagtgc aggcatcact tttgtccagc catcaaagca caaaatacga
    38881 ccgcgtgctt tcaggcggaa atcgcccgca ccaacggtca gcgtcgtgga gtcatatttc
    38941 gctggggtca tctggcaggc aacgaactga cgccagatta actggtacag tttctgcgca
    39001 tctgcttcca tatccttcag cgattccgcc atcacattga cgtcagaagg acgaatcgct
    39061 tcgtgcgctt cctgtgagtt ttctttgctg gcgtactgat tcggactttc cggcagatat
    39121 ttcttaccaa aattatcgct gatataaccg cgaaccatat ttaccgcgtc ctgactcagg
    39181 ttagtggagt cggtacgcat gtaagtgata tagcctgctt catacaaacg ctgcgccatc
    39241 atcatggttt ttttcacgcc aaaaccaaga cgggtgctgg cagcttgttg cagcgtagag
    39301 gtaataaaag gagcgccagg tttactggtt gtcggtttgt cttcacgttc cagcacgctg
    39361 tagcgcgctt tttccagcag acttaccgca gcctgagttt gttctttgtt gaccggacgg
    39421 aacggtttgt cattctgatg agtcacctgc aacgccaacg cttcaccaga tggcgtagtc
    39481 gtgctggcat cgacttccca gaactcttcc ggtacgaacg ctttaatttc acgctcacgc
    39541 tctaccacca ggcggaccgc caccgactgc acacgaccgg cagacagacc acgagcgatc
    39601 tttttccaca gcagcggcga aaccatatac cccaccacgc ggtccataaa gcgacgcgcc
    39661 tgctgggcat taacacgatc aatattcagc tcacccggtt tgttaaatgc ctggcggatc
    39721 gcgtttttag taatttcgtt aaacaccact cggctatagc gcgcatcatc accaccaatc
    39781 acttcccgca ggtgccatgc aatggcttcc ccttcgcggt caaggtcggt tgcgagatag
    39841 atgtggtcgg ctttttcagc cagttgtttc agttcagaga cgaccttctc tttaccaggc
    39901 aacacttcat agtgcgcctc ccaattgtgc cacgggtcaa cccccatacg gttgacgaga
    39961 gcgccacgtt catccttttt aggcttttta gccgtcttgg tggaggtaga gtcggcactc
    40021 tttttggcag ctgagccact ggtcggcaaa tcgcggatgt gaccgacgct ggatttcacc
    40081 acgtagtcac aacccagata cttgttgatc gttttggctt ttgccgggga ctcaacgatg
    40141 acaagagctt tacccatatt caccttacct aatttgattc ttccaggaat gcgtcgcacg
    40201 ttgataaacc ttccactggc aacgagttac cgatatggac tcgctctctg cggatatcaa
    40261 ccccttgtcg tgactctgtt cacgaaaatt caggtgactg agttaacagg tcttggccta
    40321 caggcgctat agcacgacgg aattttggtc gaatgtcaag caaatctatt gccagattga
    40381 cgaaagcgtc acactgtacc tgataaaatc cgatacgcaa ctttattagc atgcaaaatc
    40441 caaagttgcc agtaaggcgc tcttgccacc agcgtggtat atttgtctgc aagccatgcc
    40501 tggcgtagac taatgtcctt gtttaaggag taaataatga acaaagagac acaacccatc
    40561 gaccgcgaaa cgctgttgaa agaggccaac aaaatcattc gtgagcatga ggatacgctc
    40621 gcagggattg aagccaccgg cgtcacgcag cgtaatggcg tgctggtatt taccggcgac
    40681 tactttttag atgaacaagg attgccaaca gccaaaagca ccgcggtgtt taatatgttc
    40741 aaacacctgg cccatgtgct ttcagaaaaa tatcatctgg ttgattaaag caaaacgcga
    40801 ggtcttagcc tcgcgtttgt cttttacatc aatggctttt gaccccgctg ccaccagcgt
    40861 agcaacaatc gatcggcgct ctctgccgcg ctgccggtga atcggtcaat gagtcgttta
    40921 cgctgcatat agcgtacatt aaccacttca cggccttcca tcaggctaag aataacttca
    40981 tcactggtgt tgatttcatc aaccaggcct ttctctaccg cctgttgtcc gtaccagtgt
    41041 tcacccgttg ccacctgttc aatatccaga gacggacgca tacgcttcac aaaatcttta
    41101 aataactgat gcgtttcgtt cagctcttcg cggaatttct cccgcccttc ttcggtattt
    41161 tcacccagca aggtcagcgt gcgcttatac tgcccggcgg tgtgcagttc gatatcaata
    41221 tctttgcttt tcaggaagcg gttaaagttg ggcatttgcg ccaccacccc aatggaaccc
    41281 acaatagcaa acggtgcgga aacaattttg tccgccacac aggccatcat gtaaccgccg
    41341 ctggcagcga ctttgtctac cgtaacagtt aaaggaatgt ttttatcacg cagacgctgc
    41401 agctgcgaag ccgccaaccc gtaaccatgc accatgccac cagggctttc cagacggagc
    41461 acaacctgat cctgcggttt gaatgctgcg agtaccgccg ttatctcttc acgtagcgag
    41521 ttcacttcat gggcgtccat gctgccttta aaatccagca cccagacgcg aggtttactg
    41581 tcagttgcca cctcgcccag tttggctttc gcttttgctg ctttcgcttc ttgcttgtgc
    41641 tttttcttct gcgctttgtg ccactgtttt tgctgatgtg agtccatcag cgccgcggcc
    41701 agttcttctt tcatctcctt atactgttcg ctgagattgt tgacccgtaa ctcgccacgc
    41761 tggcgtttat tacgttgagc aacattgaca ataatggcgg caatcgccgc aatcgctagc
    41821 acaacggtaa cgattttagc caaaaacaaa ccatattcag acaacaattc cacgagtccc
    41881 caccttggtt aaacaacgct ttggctcaca gtttacaaca ggcatgttaa gccgtctcgc
    41941 agagatgtta ttgcagcgaa agtatgacaa agtatccatt ttgcggtgtt atcgccccag
    42001 gctgattgaa atgcccttct gtttcaggca taaagcccca aagtcataaa gtacactggc
    42061 agcgcgacag acaacgtcgt tgaggagtag ccatgcatta ccagccaaaa caagatttac
    42121 tcaatgatcg cattatcctg gtgacgggag ccagcgatgg gattggtcgt gaagccgcga
    42181 tgacgtatgc acgctatggt gcgacagtga ttctgttggg ccgtaatgaa gaaaaattac
    42241 gtcaggtagc cagccacata aacgaagaaa ctgggcgtca gccacagtgg tttattctcg
    42301 atttgctgac ctgcacgtcc gaaaattgcc aacaactggc acagcgcatt gtcgttaatt
    42361 atccgcgtct ggatggtgtt ttgcataatg ccggattgct cggcgatgtt tgcccaatga
    42421 gcgaacaaaa tccgcaggtc tggcaggacg tcatgcaggt caacgttaat gccaccttta
    42481 tgctcaccca ggcactgctt cctttattac tcaaatcgga cgccggttca ctggtcttta
    42541 cttcatcaag cgttggacgt cagggacgag ccaactgggg tgcatatgca gcgtcgaaat
    42601 ttgccaccga agggatgatg caggtactgg ccgatgaata tcagcagcgc ctgcgcgtca
    42661 actgcattaa cccaggcggt acgcgcaccg caatgcgcgc aagcgccttc ccgaccgaag
    42721 atccacagaa acttaaaaca cccgctgata tcatgccgct ctacctctgg ctgatgggcg
    42781 atgacagccg ccgtaaaacc ggcatgacct ttgacgccca accgggccgt aaaccaggaa
    42841 tttcccaatg agtgatgaac gctaccaaca gcgtcagcag cgagtgaaag aaaaagtaga
    42901 tgctcgtgtg gcccaggccc aggatgaacg cggtattatc atcgtcttta ccggcaatgg
    42961 aaaaggcaaa accaccgcgg catttggtac ggcaacacgc gcagttggtc acggaaaaaa
    43021 agtgggcgtc gtgcagttta ttaaaggcac ctggcctaat ggcgaacgca atctgctgga
    43081 gccacatggc gttgagtttc aggtgatggc aacgggcttt acctgggata cacaaaaccg
    43141 cgagtctgat accgccgcct gccgcgaagt ctggcaacat gcaaagcgga tgcttgctga
    43201 ttcctcactg gatatggttt tgcttgatga actgacgtat atggtggcgt atgactattt
    43261 gccactggaa gaagtggtgc aggcgttaaa tgaacgtcca catcaacaga cggtgattat
    43321 cacgggtcga ggctgtcatc gggatattct tgaactggcg gatacggtaa gtgagctgcg
    43381 ccccatcaga catgcgtttg atgccggtgt taaggcgcaa atagggattg attattaatc
    43441 tgtaaaaatg ccggatatga cgtccggcat actttgatta accgttatta cgcccgccag
    43501 aacgacgact gccgctcacc tgactgtgac gtttcaccgc acgacgaatc tgattagcct
    43561 tcatgcgacg acggtctttt tctacagcga ctttagagct ggtttctggc ggaagctcca
    43621 ccagttcgcg cagatagtta gtctgggcga ggtccagctc cgtccagcca ccgcgtggca
    43681 gaccttttgg cagtgggata tcaccgtaac gaacacggat caggcggctc acctgcacac
    43741 caaccgcttc ccacagacga cgaacttcac ggttacggcc ttcggtcaga gtcacgttgt
    43801 accactggtt gatcccttcg ccgccgctga acttgatggt tttaaaagct gccggaccat
    43861 cttccagttg cacgccacga ctcaaatcac gcagtttcgc gtcgtcaacc tgaccaaata
    43921 cacgcacggc gtattcacgt tcaacttcac ggcttggatg cattaaacgg tttgccagtt
    43981 caccatcggt ggtgaacagc agcagaccac aggtgttaac gtccagacga cctacggcaa
    44041 tccagcgtgc gccacgcagc tttggcagac ggtcaaacac cgtcggacgc ccttccggat
    44101 cgttacgggt acacaactca ccttccggtt tgtaataggc cagaacgcga caaatttgtt
    44161 cagcggactc acgtaccgaa atcagatgac catcgatacg aattttcagc ccaggggtaa
    44221 cttcaacgcg atcgccgagt ttagcaattt tgccatcaac actcacacga ccggcttcaa
    44281 taatagattc gatttcacgg cgggagccgt ggccagcgcg ggccagcact ttctgtaatt
    44341 tttcgctcat agagcttcct tttggtgtcg ccttcacagg cgtcgaacag gagaatcaat
    44401 ggcgccgtat agcgccattt taaggccgcg tagtatacag agttacgcac ttataagaaa
    44461 ggcttcacat cacctacgcc ttcacgcacc acgaccggcg tatcatcggt aagatcaata
    44521 accgttgttg gtttctgccc gagatagccg ccgtgaataa tcaaatctac ctgtttttcc
    44581 agacggtctt taatttcttc gggatcggat tcggtaaatt cgctgcctgg cagcattagc
    44641 gaagtggaaa gcatcggttc gcccagtgct tcaagcaacg cctgcgcaat cgggttagaa
    44701 ggcacacgca taccgatggt tttgcgtttc tcctgcaaca gacggcgtgg cacttccttc
    44761 gtccccttca ggatgaaggt atagttgccc ggcgtgttgt ttttcatcaa acgaaacgca
    44821 acgttatcca cgaatgaata ggtcgagagt tcagaaagat cgcgacacat cagggtaaag
    44881 ttgtgaccgt ccggcagctg gcgaatacga caaatacgct ccatggcgtt tttatcttca
    44941 attttacagc cgagcgcata gccggaatca gttggataga caatcacccc gcctttacgc
    45001 acgatctcca ccgcctggtt gatcagacgt tgctgcgggt tatcaggatg aatataaaaa
    45061 aactggctca taattccctc tctgtggtgt tctgcggctg ttcccatagc tgccagacgc
    45121 cttcaacgcc tgcgggcagc cagagtttac gacccagttc gatccacggg catggctgat
    45181 gaaaatcaga tccttgtgat gcccataaat gatgctgacg cgcaagtgtg gccagctggg
    45241 tgcgttcatt gggcgattgc tggcactgcg cgacttccat cgcgtcgccg tggtgttcgg
    45301 caaaatgcgc aaccaatctt ttcagccatt tagcactaag attgtaccgc ccaggatgag
    45361 ctagcaccgc cttaccgcca gaatgatgaa tgacatcaat agcttgttct attgtacacc
    45421 actgtggcgg aacgtatccg gttttcccgc gcgccagata ctttttaaag acatctgcca
    45481 ttgaactggc tttgccgcac tccacaagga aacgcgcaaa atgaccgcgc gtcactgcgc
    45541 cgccctgcgc cagtcgttgt gcgccttcca gcgccccagg tatttgcgct ttttcaagtc
    45601 tttcggcaat ttgctgcgcc cgctgattgc gccgttctgt ctgctgcgcg aggaactcac
    45661 acattagcgg atgagtaata tcaatattca gcccgacaat atgaatttca tgattttccc
    45721 agaccgtgga aatttccacg ccgggaataa gattcagcgc cagtcccgaa cgtgaaattt
    45781 cctctcttgc tggcgcgatt gcagctgtgg tgtcatggtc ggtgatcgcc agggtgccga
    45841 cgcgcatctc gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg
    45901 tggtatggct gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc
    45961 gttctggata atgttttttg cgccgacatc ataacgggtc tggcaaatat tctgaaatga
    46021 gctgttgaca attaatcatc gaaccagtta actagtgcgc aagttcacgt aaaaagggta
    46081 tcgacaatga aagcaatttt cgtactgaaa ggttggtggc gcacttcctg aaacgggcag
    46141 tgtattcacc atgcgtaaag caatcagata cccagcccgc ctaatgagcg ggcttttttt
    46201 tgaacaaaat tagagaataa caatgcaaac acaaaaaccg actctcgaac tgctaacctg
    46261 cgaaggcgct tatcgcgaca acccgactgc gctttttcac cagttgtgtg ggaatcgtcc
    46321 ggcaacgctg ctgctggaat ccgcagatat cgacagcaaa gatgatttaa aaagcctgct
    46381 gctgatagac agtgcgctgc gcattacagc attaggtgac accgtcacaa ttcaggcact
    46441 ttccggcaat ggcgaagccc tgctggcact actggataac gccctgcctg cgggtgtgga
    46501 aagtgaacaa tcaccaaact gccgtgtgct gcgcttcccc cctgtcagtc cactgctgga
    46561 tgaagacgcc cgcttatgct ccctttcggt ttttgacgct ttccgtttat tgcagaatct
    46621 gttgaatgta ccgaaggaag aacgagaagc aatgttcttc ggcggcctgt tctcttatga
    46681 ccttgtggcg ggatttgaag atttaccgca actgtcagcg gaaaataact gccctgattt
    46741 ctgtttttat ctcgctgaaa cgctgatggt gattgaccat cagaaaaaaa gcacccgtat
    46801 tcaggccagc ctgtttgctc cgaatgaaga agaaaaacaa cgtctcactg ctcgcctgaa
    46861 cgaactacgt cagcaactga ccgaagccgc gccgccgctg ccagtggttt ccgtgccgca
    46921 tatgcgttgt gaatgtaatc agagcgatga agagttcggt ggcgtagtgc gtttgttgca
    46981 aaaagcgatt cgcgctggag aaattttcca ggtggtgcca tctcgccgtt tctctctgcc
    47041 ctgcccgtca ccgctggcgg cctattacgt gctgaaaaag agtaatccca gcccgtacat
    47101 gttttttatg caggataatg atttcaccct atttggcgcg tcgccggaaa gctcgctcaa
    47161 gtatgatgcc accagccgcc agattgagat ctacccgatt gccggaacac gcccacgcgg
    47221 tcgtcgcgcc gatggttcac tggacagaga tctcgacagc cgtattgaac tggaaatgcg
    47281 tactgaccat aaagagctgt ctgaacatct gatgctggtt gatctcgccc gtaatgatct
    47341 ggcacgcatt tgcacccctg gcagccgcta catcgccgat ctcaccaaag ttgaccgtta
    47401 ttcctatgtg atgcacctcg tctctcgcgt agtcggcgaa ctgcgtcacg atcttgacgc
    47461 cctgcacgct tatcgcgcct gtatgaatat ggggacgtta agcggtgcgc cgaaagtacg
    47521 cgctatgcag ttaattgccg aggcggaagg tcgtcgccgc ggcagctacg gcggcgcggt
    47581 aggttatttc accgcgcatg gcgatctcga cacctgcatt gtgatccgct cggcgctggt
    47641 ggaaaacggt atcgccaccg tgcaagcggg tgctggtgta gtccttgatt ctgttccgca
    47701 gtcggaagcc gacgaaaccc gtaacaaagc ccgcgctgta ctgcgcgcta ttgccaccgc
    47761 gcatcatgca caggagactt tctgatggct gacattctgc tgctcgataa tatcgactct
    47821 tttacgtaca acctggcaga tcagttgcgc agcaatggtc ataacgtggt gatttaccgc
    47881 aaccatattc cggcgcagac cttaattgaa cgcctggcga cgatgagcaa tccggtactg
    47941 atgctttctc ctggccccgg tgtgccgagc gaagccggtt gtatgccgga actcctcacc
    48001 cgcttgcgtg gcaagctgcc cattattggc atttgcctcg gacatcaggc aattgtcgaa
    48061 gcttacgggg gctatgtcgg tcaggcgggc gaaattcttc acggtaaagc gtcgagcatt
    48121 gaacatgacg gtcaggcgat gtttgccgga ttaacaaacc cgctgccggt ggcgcgttat
    48181 cactcgctgg ttggcagtaa cattccggcc ggtttaacca tcaacgccca ttttaatggc
    48241 atggtgatgg cagtacgtca cgatgcggat cgcgtttgtg gattccagtt ccatccggaa
    48301 tccattctca ccacccaggg cgctcgcctg ctggaacaaa cgctggcctg ggcgcagcag
    48361 aaactagagc cagccaacac gctgcaaccg attctggaaa aactgtatca ggcgcagacg
    48421 cttagccaac aagaaagcca ccagctgttt tcagcggtgg tgcgtggcga gctgaagccg
    48481 gaacaactgg cggcggcgct ggtgagcatg aaaattcgcg gtgagcaccc gaacgagatc
    48541 gccggggcag caaccgcgct actggaaaac gcagcgccgt tcccgcgccc ggattatctg
    48601 tttgctgata tcgtcggtac tggcggtgac ggcagcaaca gtatcaatat ttctaccgcc
    48661 agtgcgtttg tcgccgcggc ctgtgggctg aaagtggcga aacacggtaa ccgcagcgtc
    48721 tccagtaaat ccggctcgtc ggatctgctg gcggcattcg gtattaatct tgatatgaac
    48781 gccaataaat cgcgccaggc gctggacgag ttaggcgtat gtttcctctt tgcgccgaaa
    48841 tatcacaccg gattccgcca cgcgatgccg gttcgccagc aactgaaaac ccgcaccctg
    48901 ttcaatgtgc tggggccatt gattaacccg gcgcatccgc cgctggcgtt aattggtgtt
    48961 tatagtccgg aactggtgct gccgattgcc gaaaccttgc gcgtgctggg gtatcaacgc
    49021 gcggcggtgg tgcacagcgg cgggatggat gaagtttcat tacacgcgcc gacaatcgtt
    49081 gccgagctgc atgacggcaa aattaagagc tatcaattga ccgctgaaga ttttggcctg
    49141 actccctacc accaggagca actggcaggc ggaacacctg aagaaaaccg tgacatttta
    49201 acacgcttgt tacaaggtaa aggcgacgcc gcccatgaag cagccgtcgc tgcgaacgtc
    49261 gccatgttaa tgcgcctgca tggccatgaa gatctgcaag ccaatgcgca aaccgttctt
    49321 gaggtactgc gcagtggttc cgcttacgac agagttaccg cgctggcggc acgagggtaa
    49381 atgatgcaaa ccgttttagc gaaaatcgtc gcagacaagg cgatttgggt agaaacccgc
    49441 aaacagcagc aaccgctggc cagttttcag aatgaggttc agccgagcac acgacatttt
    49501 tatgatgcgc tacagggtgc acgcacggcg tttattctgg agtgtaaaaa agcgtcgccg
    49561 tcaaaaggcg tgatccgtga tgatttcgat ccggcacgca ttgccgccat ttataaacat
    49621 tacgcttcgg caatttcggt gctgactgat gagaaatatt ttcaggggag ctttgatttc
    49681 ctcctcatcg tcagccaaat cgccccgcag ccgattttat gtaaagactt cattatcgac
    49741 ccttaccaga tctatctggc gcgctattac caggccgatg cctgcttatt aatgctttca
    49801 gtactggatg acgaccaata tcgccagctt gcagccgtcg ctcacagtct ggagatgggg
    49861 gtgctgaccg aagtcagtaa tgaagaggaa ctggagcgcg ccattgcatt aggggcaaag
    49921 gtcgttggca tcaacaaccg cgatctgcgt gatttgtcga ttgatctcaa ccgtacccgc
    49981 gagcttgcgc cgaaactggg gcacaacgta acggtaatca gcgaatccgg catcaatact
    50041 tacgctcagg tgcgcgagtt aagccacttc gctaacggtt ttctgattgg ttcggcgttg
    50101 atggcccatg acgatttgca cgccgccgtg cgccgggtgt tgctgggtga gaataaagta
    50161 tgtggcctga cgcgtgggca agatgctaaa gcagcttatg acgcgggcgc gatttacggt
    50221 gggttgattt ttgttgcgac atcaccgcgt tgcgtcaacg ttgaacaggc gcaggaagtg
    50281 atggctgcgg caccgttgca gtatgttggc gtgttccgca atcacgatat tgccgatgtg
    50341 gtggacaaag ctaaggtgtt atcgctggcg gcagtgcaac tgcatggtaa tgaagatcag
    50401 ctgtatatcg atacgctgcg tgaagctctg ccagcacatg ttgccatctg gaaagcatta
    50461 agcgtcggtg aaaccctgcc cgcccgcgag tttcagcacg ttgataaata tgttttagac
    50521 aacggccagg gtggaagcgg gcaacgtttt gactggtcac tattaaatgg tcaatcgctt
    50581 ggcaacgttc tgctggcggg gggcttaggc gcagataact gcgtggaagc ggcacaaacc
    50641 ggctgcgccg gacttgattt taattctgct gtagagtcgc aaccgggcat caaagacgca
    50701 cgtcttttgg cctcggtttt ccagacgctg cgcgcatatt aaggaaagga acaatgacaa
    50761 cattacttaa cccctatttt ggtgagtttg gcggcatgta cgtgccacaa atcctgatgc
    50821 ctgctctgcg ccagctggaa gaagcttttg tcagcgcgca aaaagatcct gaatttcaag
    50881 cccagttcaa cgacctgctg aaaaactatg ccgggcgtcc aaccgcactg accaaatgcc
    50941 agaacattac agccgggacg aacaccacgc tgtatctcaa gcgtgaagat ttgctgcacg
    51001 gcggcgcaca taaaaccaac caggtgctgg ggcaggcgtt gctggcgaag cggatgggta
    51061 aaaccgaaat catcgccgaa accggggccg gtcagcatgg cgtggcgtcg gcccttgcca
    51121 gcgccctgct cggcctgaaa tgccgtattt atatgggtgc caaagacgtt gaacgccagt
    51181 cgcctaacgt ttttcgtatg cgcttaatgg gtgcggaagt gatcccggta catagcggtt
    51241 ccgccacgct gaaagatgcc tgtaatgagg cgctacgcga ctggtccggc agttatgaaa
    51301 ccgcgcacta tatgctgggc accgcggctg gcccgcatcc ttatccgacc attgtccgtg
    51361 agtttcagcg gatgattggc gaagaaacca aagcgcagat tctggaaaga gaaggtcgcc
    51421 tgccggatgc cgttatcgcc tgtgttggcg gcggttcgaa tgccatcggc atgtttgctg
    51481 atttcatcaa tgaaaccaac gtcggcctga ttggtgtgga gcctggtggt cacggtatcg
    51541 aaactggcga gcacggcgca ccgctaaaac atggtcgcgt gggcatttat ttcggtatga
    51601 aagcgccgat gatgcaaacc gaagacgggc agattgaaga atcttactcc atctccgccg
    51661 gactggattt cccgtctgtc ggtccacaac acgcgtatct taacagcact ggacgcgctg
    51721 attacgtgtc tattaccgat gatgaagccc ttgaagcctt caaaacgctg tgcctgcacg
    51781 aagggatcat cccggcgctg gaatcctccc acgccctggc ccatgcgctg aaaatgatgc
    51841 gcgaaaaccc ggataaagag cagctactgg tggttaacct ttccggtcgc ggcgataaag
    51901 acatcttcac cgttcacgat attttgaaag cacgagggga aatctgatgg aacgctacga
    51961 atctctgttt gcccagttga aggagcgcaa agaaggcgca ttcgttcctt tcgtcacgct
    52021 cggtgatccg ggcattgagc agtcattgaa aattatcgat acgctaattg aagccggtgc
    52081 tgacgcgctg gagttaggta tccccttctc cgacccactg gcggatggcc cgaccattca
    52141 aaacgccact ctgcgcgcct ttgcggcagg tgtgactccg gcacaatgtt ttgaaatgct
    52201 ggcactgatt cgccagaaac acccgaccat tcccattggc ctgttgatgt atgccaatct
    52261 ggtgtttaac aaaggcattg atgagtttta tgcccagtgc gagaaagtcg gcgtcgattc
    52321 ggtgctggtt gccgatgtgc cagttgaaga gtccgcgccc ttccgccagg ccgcgttgcg
    52381 ccataatgtc gcacctatct tcatctgccc gccaaatgcc gatgacgacc tgctgcgcca
    52441 gatagcctct tacggtcgtg gttacacata tttgctgtca cgagcgggcg tgaccggcgc
    52501 agaaaaccgc gccgcgttac ccctcaatca tctggttgcg aagctgaaag agtacaacgc
    52561 tgcacctcca ttgcaggggt ttggtatttc cgccccggat caggtaaaag cagcgattga
    52621 tgcaggagct gcgggcgcga tttctggttc ggccattgtt aaaatcatcg agcaacatat
    52681 taatgagcca gagaaaatgc tggcggcact gaaagttttt gtacaaccga tgaaagcggc
    52741 gacgcgcagt taatcccaca gccgccagtt ccgctggcgg cattttaact ttctttaatg
    52801 aagccggaaa aatcctaaat tcatttaata tttatctttt taccgtttcg cttaccccgg
    52861 tcgaacgtca acttacgtca tttttccgcc caacagtaat ataatcaaac aaattaatcc
    52921 cgcaacataa caccagtaaa atcaataatt ttctctaagt cacatattcc tcaggtaatt
    52981 gttaatatat ccagaatgtt cctcaaaata tattttccct ctatcttctc gttgcgctta
    53041 atttgactaa ttctcattag cgactaattt taatgagtgt cgacacacaa cactcatatt
    53101 aatgaaacaa tgcaacgcaa cgggagaaat aacatggccg aacatcgtgg tagttcagga
    53161 aatttcgccg aagaccgtga gaaggcatcc gacgcaggcc gtaaaggcgg tcagcatagc
    53221 ggcggtaatt ttaaaaatga tccgcaacgc gcatctgaag cgggtaaaaa aggcggtcaa
    53281 caaagcggtg gtaataaatc aggcaaatcc tgattcaccg ttaattatta atatcagctg
    53341 ataaagataa ttgcatctgc gggccgtatg gctcgcagtg atttcactgg agaaaaatat
    53401 gaatatgaag accattgaag atgtatttat tcaactgctt tcagatacct acagcgcaga
    53461 aaaacaatta acccgggcac tggcaaaact cgcaagagca acatcaaatg aaaaattaag
    53521 tcaggctttt catgcgcacc tcgaggaaac tcatggacag attgaacgaa ttgatcaagt
    53581 tgtggaatca gaatcaaatc tgaaaattaa gcgcatgaaa tgtgtggcaa tggaaggtct
    53641 tattgaagaa gctaatgagg tcatcgaaag taccgagaaa aacgaagtgc gtgatgccgc
    53701 actgattgcc gcagcacaga aagtcgagca ttatgagatt gccagttacg ggacattagc
    53761 gacgctggct gaacaattag gttaccgtaa agcagcgaag cttctgaaag aaaccctgga
    53821 agaagaaaag gccaccgaca tcaaactgac tgatctggcc cttaataacg taaataagaa
    53881 agccgaaaat aaagcctgaa atatgaattt taacttttag tcattttata aagaggacat
    53941 tttcatgaat cgtattgaac attatcatga ctggttacgt gacgcccacg caatggaaaa
    54001 gcaagccgaa tctatgcttg aatccatggc cagccgtata gataattatc ctgaactacg
    54061 cgctcgtatt gaacaacatc ttagtgaaac caaaaaccag attgttcaac tggaaactat
    54121 tcttgatcgt aatgacattt cacgttcagt cattaaagat tccatgagta aaatggctgc
    54181 gcttgggcag tcaatcggtg gtatattccc ttctgatgaa atagttaaag gctctattgg
    54241 cggatatgtc ttcgagcaat ttgaaatcac ctgttacacc tcactattag cagcagcaaa
    54301 aaatgccggt gatacagtct caattccgat catcgaagcg attttaaatg aggaaaagca
    54361 aatggccgac tggctgattc agcatattcc gcaaacaact gagaaatttt taattcgttc
    54421 tgaaactgat ggcgtagaag cgaagaaata atacccttta taccatgtcc ttattgaccc
    54481 cgtatattac ggggtcgttt ttgtgcggaa ttaaaaacga tatcctgctg agaacataaa
    54541 cacccacgga tcgaggcgta cgctatcgtg ttgctgtgca ccgcctagct tataattggc
    54601 ggtggtatcg atatccatgt accacactga catgttaacc aaccagtcac ggttaatcag
    54661 ataatcaacc cccacctgcc cggcagctcc ccaggaatct ttcagactga gatcggaaag
    54721 ccccgcctct ttgccatgat cgttaaatcc attatcaaag aaggtggtgt agttaatacc
    54781 ggccccaacg taaggacgga atttgctgct ggcatcacca aaataccact gcgccatcag
    54841 tgttggcggc agatggtgaa cggttgcaat atcgccggtc gcccgggtgc cgattttatg
    54901 gcggaacggc gtcgctgcca gtaattccac accaatgttg tcggtcgcca tataagtaaa
    54961 cgtcaagccc agttgagtgt tattggtcac gctgaatcca cccagacttc ctaacgtacc
    55021 accagcacct tctgttggac gtacggttgc agaacctgca cgcataaaaa attcgcctgc
    55081 ttcatgcgca aaggcactgc cagaaagaag agttgttact gccaaagccg ccactgttaa
    55141 ctttttcata tccgctccgt cgttatggtt ataaaaacgt gacgaatata cctacattgg
    55201 agtaacaaat gatcctacat agatcacatt acatatgata attcaccatt tattgatctg
    55261 gattaattta gaagttgcat tgagaatcct ggttaatgtg atttaaatca atttttaaat
    55321 attttctcta cgcaaatatt aactcgatcg ccacttgccg cggtttgccc acgcctgaag
    55381 gccagcaatt acctccgctt tacaaagata tgcttttcca tacaagacct gatgctgcca
    55441 cccgaggctt aaccagggta caattgcccg ctaattaaca cctgcataaa actcaaggag
    55501 agtgcatgtc tatcacggcg cagtccgtat accgtgacac cggaaatttc ttccgtaatc
    55561 aatttatgac cattctgttg gtatcgttgc tatgtgcgtt tatcacagtg gtgttagggc
    55621 atgttttctc acccagtgat gcacagcttg cgcagctcaa tgacggcgtg cccgttagcg
    55681 gcagtagtgg gttgttcgac ctggttcaga atatgtcacc ggaacagcaa caaattttgc
    55741 tgcaggcttc agcggcgtcc actttttcag aattaatcgg taacgccatt ctcgccggag
    55801 gcgtaatatt aattatccag ctggtgtctg cgggtcagag agtcagtgcg ctgcgagcta
    55861 ttggtgccag tgcgccgata ttgcccaagt tatttattct gatttttctg actacccttt
    55921 tagtacagat tggcatcatg ctggtggtcg ttccgggaat tatcatggcc attttactgg
    55981 ccctggcacc ggtgatgctg gtacaggaca aaatgggcat ttttgcctcg atgcgtagca
    56041 gtatgcggct gacttgggcg aatatgcgtc tggtggcacc cgcagtactg agctggttgc
    56101 tggcaaaaac actgttgctg ctttttgcct cttcttttgc cgcattaacc ccggaaattg
    56161 gtgccgtact ggcgaacacc ttgagcaacc tgatttctgc catattgctc atctatctgt
    56221 tccgcctgta tatgttgatt cgccaataac cttaagcatt tgattacgga atcgtaaaat
    56281 gaagcagttt cttgattttt taccgctggt tgtctttttc gcgttttaca agatttatga
    56341 catctatgcg gctactgcag cgctgatcgt cgccacggcg attgtgctta tctatagctg
    56401 ggttcgcttt cgtaaggttg agaagatggc cctgatcact tttgttctgg tggtcgtctt
    56461 cggtggcttg acgctgttct tccacaatga tgagtttatt aaatggaagg tgacagtcat
    56521 ttatgctctg tttgcgggag ccctgttagt cagccaatgg gtgatgaaaa agccgctaat
    56581 tcagcggatg ctgagtaaag aactcacgct gccgcaaccg gtatggtcga agctaaatct
    56641 ggcctgggct gttttcttta tcctttgcgg tctggcaaac atctacatcg cattctggct
    56701 gccgcaaaat atttgggtca actttaaagt ctttggcctg accgccctta ccttaatctt
    56761 cacattgtta agcggtatct atatctaccg ccacatgccg caggaagata aatcctaacc
    56821 agactgccag tccttcgggg ctggcgctgt atctcctctg ttctcctcag gaaaatcata
    56881 gtagcatcgc gcctgtgatt ttccttttaa gtcggtttta ccatgtctac aacacataac
    56941 gtccctcagg gcgatcttgt tttacgtact ttagccatgc ccgccgatac caatgccaat
    57001 ggtgacatct ttggtggttg gttaatgtca caaatggata ttggcggcgc tattctggca
    57061 aaagaaattg cccacggtcg cgtagtgact gtgcgggttg aaggaatgac tttcttacgg
    57121 ccggttgcgg tcggcgatgt ggtgtgctgc tatgcacgct gtgtccagaa agggacgaca
    57181 tcggtcagca ttaatattga agtatgggtg aaaaaagtag cgtctgaacc aatcgggcaa
    57241 cgctataaag cgacagaagc attatttaag tatgtagcgg ttgatcctga aggaaaacct
    57301 cgcgccttac ctgttgagta atagtcaaaa gcctccggtc ggaggctttt gactttctgc
    57361 ttactgaatt tcggtggtgc cgttaatttt aaacaggata ttcaccacaa tcccactgcc
    57421 tggcttaccc ggctcataac gccatctgcg catcgcattt ttcacctcac gctcaaacat
    57481 gttcgcaggc ttggctgaga ggatttgtac gttatccacg cgaccatccg gcgtgacgtc
    57541 aaatttaact ttaacctgcc cttcaatgcg caatgcctgt gctcgtgccg gatactgcgg
    57601 ctgattacgg cttaatgcgc gtggtcctga agccacactg gtaaccggct tgctggttgc
    57661 agccgttgcc gtacttgatg tggggcgcgc gggtgccgta ttttcaaacg gtgatgccgg
    57721 acgcgactct acgggtttga catcgcgttt tggctgctcc tgtacctttt tcaccggctt
    57781 cggttttggc ttcggtttcg gcttcggctt ttcaatgacc accggtgctt cttttggcgg
    57841 ttcggggatc ggctcaggtt ccggttctgg ctctaccacc ggctccggtg gcggctgaac
    57901 ggcttgtggc ggttcgagat cagcaggcgc aaccatcgtg acagaaattg gttgcgcagg
    57961 cgcaggtagt tcaataacct gatgtaccga ggtatagagc agccccgcca caacagcacc
    58021 atgaatgcag accgaaagta acgtcggcca ggggaagcgg cgaggtaaat caagggttat
    58081 tgaagtcata atcatttcag tagaaaaact aggcctcgat tttaaatgca aatagcaatc
    58141 atattcaata aggcaagacg atcaggatca gtgaaaatgt catttttggc gtaattaccc
    58201 ttactttatg catgacctta acaaagacat catctttccg ttgcagatct tcgccctttc
    58261 aaataacgta ctttacaact ttcccgaaca aggagttgtg cccgtgttgt atgttattta
    58321 cgctcaagac aaagctgata gcctcgaaaa acgcctttcc gttcgtccgg cacatttagc
    58381 acgtttacag ttactgcatg atgaaggtcg tttgttgaca gctggtccaa tgccagcggt
    58441 agacagtaac gatccagggg ctgcgggatt tactggctca accgttattg ctgaatttga
    58501 atcgctggaa gcagctcagg cctgggccga tgctgacccg tacgtagcgg caggagtcta
    58561 tgaacacgtt tcagtgaaac catttaagaa agtgttctga g
//
LOCUS       DAEEGA010000015        58080 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEEGA010000015 DAEEGA010000000
VERSION     DAEEGA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58080
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.015"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            699..1127
                     /gene="uspC"
                     /locus_tag="KLH46_000948"
     CDS             699..1127
                     /gene="uspC"
                     /locus_tag="KLH46_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBE0377737.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASNPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1134..2548)
                     /gene="otsA"
                     /locus_tag="KLH46_000949"
                     /pseudo
     CDS             complement(1134..2548)
                     /gene="otsA"
                     /locus_tag="KLH46_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(2523..3323)
                     /gene="otsB"
                     /locus_tag="KLH46_000950"
     CDS             complement(2523..3323)
                     /gene="otsB"
                     /locus_tag="KLH46_000950"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBE0377738.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(3490..4476)
                     /gene="araH"
                     /locus_tag="KLH46_000951"
     CDS             complement(3490..4476)
                     /gene="araH"
                     /locus_tag="KLH46_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBE0377739.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGIPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4491..6005)
                     /gene="araG"
                     /locus_tag="KLH46_000952"
     CDS             complement(4491..6005)
                     /gene="araG"
                     /locus_tag="KLH46_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HBE0377740.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6075..7064)
                     /gene="araF"
                     /locus_tag="KLH46_000953"
                     /pseudo
     CDS             complement(6075..7064)
                     /gene="araF"
                     /locus_tag="KLH46_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            7861..8364
                     /locus_tag="KLH46_000954"
     CDS             7861..8364
                     /locus_tag="KLH46_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBE0377741.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8444..8695)
                     /locus_tag="KLH46_000955"
     CDS             complement(8444..8695)
                     /locus_tag="KLH46_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBE0377742.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8810..8896)
                     /gene="azuC"
                     /locus_tag="KLH46_000956"
     CDS             complement(8810..8896)
                     /gene="azuC"
                     /locus_tag="KLH46_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBE0377743.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9159..9482
                     /locus_tag="KLH46_000957"
     CDS             9159..9482
                     /locus_tag="KLH46_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBE0377744.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9653..10150
                     /gene="ftnA"
                     /locus_tag="KLH46_000958"
     CDS             9653..10150
                     /gene="ftnA"
                     /locus_tag="KLH46_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBE0377745.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10187..10426)
                     /locus_tag="KLH46_000959"
     CDS             complement(10187..10426)
                     /locus_tag="KLH46_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBE0377746.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10616..11827
                     /gene="tyrP"
                     /locus_tag="KLH46_000960"
     CDS             10616..11827
                     /gene="tyrP"
                     /locus_tag="KLH46_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBE0377747.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11889..12554)
                     /gene="yecA"
                     /locus_tag="KLH46_000961"
     CDS             complement(11889..12554)
                     /gene="yecA"
                     /locus_tag="KLH46_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBE0377748.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12750..12836)
                     /locus_tag="KLH46_000962"
     tRNA            complement(12750..12836)
                     /locus_tag="KLH46_000962"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12800..12802),aa:Leu,seq:taa)
     gene            complement(12849..12922)
                     /locus_tag="KLH46_000963"
     tRNA            complement(12849..12922)
                     /locus_tag="KLH46_000963"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12888..12890),aa:Cys,seq:gca)
     gene            complement(12977..13052)
                     /locus_tag="KLH46_000964"
     tRNA            complement(12977..13052)
                     /locus_tag="KLH46_000964"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13017..13019),aa:Gly,seq:gcc)
     gene            complement(13204..13752)
                     /gene="pgsA"
                     /locus_tag="KLH46_000965"
     CDS             complement(13204..13752)
                     /gene="pgsA"
                     /locus_tag="KLH46_000965"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBE0377749.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13809..15641)
                     /gene="uvrC"
                     /locus_tag="KLH46_000966"
     CDS             complement(13809..15641)
                     /gene="uvrC"
                     /locus_tag="KLH46_000966"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBE0377750.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15638..16294)
                     /gene="uvrY"
                     /locus_tag="KLH46_000967"
     CDS             complement(15638..16294)
                     /gene="uvrY"
                     /locus_tag="KLH46_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBE0377751.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVAGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16590..16766
                     /locus_tag="KLH46_000968"
     CDS             16590..16766
                     /locus_tag="KLH46_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377752.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            16753..16977
                     /locus_tag="KLH46_000969"
     CDS             16753..16977
                     /locus_tag="KLH46_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBE0377753.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17010..17630)
                     /gene="sdiA"
                     /locus_tag="KLH46_000970"
                     /pseudo
     CDS             complement(17010..17630)
                     /gene="sdiA"
                     /locus_tag="KLH46_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(17860..18612)
                     /gene="yecC"
                     /locus_tag="KLH46_000971"
     CDS             complement(17860..18612)
                     /gene="yecC"
                     /locus_tag="KLH46_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBE0377754.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(18609..19277)
                     /gene="tcyL"
                     /locus_tag="KLH46_000972"
     CDS             complement(18609..19277)
                     /gene="tcyL"
                     /locus_tag="KLH46_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBE0377755.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19292..20278)
                     /gene="dcyD"
                     /locus_tag="KLH46_000973"
     CDS             complement(19292..20278)
                     /gene="dcyD"
                     /locus_tag="KLH46_000973"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBE0377756.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(20383..21183)
                     /gene="tcyJ"
                     /locus_tag="KLH46_000974"
     CDS             complement(20383..21183)
                     /gene="tcyJ"
                     /locus_tag="KLH46_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0377757.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(21271..21822)
                     /gene="fliZ"
                     /locus_tag="KLH46_000975"
     CDS             complement(21271..21822)
                     /gene="fliZ"
                     /locus_tag="KLH46_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBE0377758.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(21868..22587)
                     /gene="fliA"
                     /locus_tag="KLH46_000976"
     CDS             complement(21868..22587)
                     /gene="fliA"
                     /locus_tag="KLH46_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBE0377759.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22752..24404)
                     /gene="fliC"
                     /locus_tag="KLH46_000977"
     CDS             complement(22752..24404)
                     /gene="fliC"
                     /locus_tag="KLH46_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBE0377760.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            24670..26076
                     /gene="fliD"
                     /locus_tag="KLH46_000978"
     CDS             24670..26076
                     /gene="fliD"
                     /locus_tag="KLH46_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBE0377761.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            26101..26511
                     /gene="fliS"
                     /locus_tag="KLH46_000979"
     CDS             26101..26511
                     /gene="fliS"
                     /locus_tag="KLH46_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBE0377762.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADVWKESLLSPSSIQDPV"
     gene            26511..26876
                     /gene="fliT"
                     /locus_tag="KLH46_000980"
     CDS             26511..26876
                     /gene="fliT"
                     /locus_tag="KLH46_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBE0377763.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26954..28441
                     /gene="amyA"
                     /locus_tag="KLH46_000981"
     CDS             26954..28441
                     /gene="amyA"
                     /locus_tag="KLH46_000981"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBE0377764.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28475..28888)
                     /locus_tag="KLH46_000982"
     CDS             complement(28475..28888)
                     /locus_tag="KLH46_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE0377765.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            29075..30280
                     /gene="yedE"
                     /locus_tag="KLH46_000983"
     CDS             29075..30280
                     /gene="yedE"
                     /locus_tag="KLH46_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBE0377766.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFPRE
                     APQTAKEIA"
     gene            30277..30510
                     /gene="yedF"
                     /locus_tag="KLH46_000984"
     CDS             30277..30510
                     /gene="yedF"
                     /locus_tag="KLH46_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBE0377767.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30617..31287
                     /locus_tag="KLH46_000985"
                     /pseudo
     CDS             30617..31287
                     /locus_tag="KLH46_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(31327..32535)
                     /locus_tag="KLH46_000986"
                     /pseudo
     CDS             complement(31327..32535)
                     /locus_tag="KLH46_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            32603..33218
                     /gene="tnpA"
                     /locus_tag="KLH46_000987"
                     /pseudo
     CDS             32603..33218
                     /gene="tnpA"
                     /locus_tag="KLH46_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            33546..34628
                     /locus_tag="KLH46_000988"
     CDS             33546..34628
                     /locus_tag="KLH46_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HBE0377768.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(35116..35914)
                     /locus_tag="KLH46_000989"
                     /pseudo
     CDS             complement(35116..35914)
                     /locus_tag="KLH46_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(35924..36475)
                     /gene="ybcL"
                     /locus_tag="KLH46_000990"
     CDS             complement(35924..36475)
                     /gene="ybcL"
                     /locus_tag="KLH46_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBE0377769.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(36644..36976)
                     /gene="emrE"
                     /locus_tag="KLH46_000991"
     CDS             complement(36644..36976)
                     /gene="emrE"
                     /locus_tag="KLH46_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBE0377770.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(37321..37635)
                     /gene="fliE"
                     /locus_tag="KLH46_000992"
     CDS             complement(37321..37635)
                     /gene="fliE"
                     /locus_tag="KLH46_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBE0377771.1"
                     /translation="MSAIQGIEGVISQLQATAMSACAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            37849..39514
                     /gene="fliF"
                     /locus_tag="KLH46_000993"
                     /pseudo
     CDS             37849..39514
                     /gene="fliF"
                     /locus_tag="KLH46_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            39507..40502
                     /gene="fliG"
                     /locus_tag="KLH46_000994"
     CDS             39507..40502
                     /gene="fliG"
                     /locus_tag="KLH46_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBE0377772.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            40495..41181
                     /gene="fliH"
                     /locus_tag="KLH46_000995"
     CDS             40495..41181
                     /gene="fliH"
                     /locus_tag="KLH46_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBE0377773.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            41181..42554
                     /gene="fliI"
                     /locus_tag="KLH46_000996"
     CDS             41181..42554
                     /gene="fliI"
                     /locus_tag="KLH46_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBE0377774.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            42576..42917
                     /gene="fliJ"
                     /locus_tag="KLH46_000997"
                     /pseudo
     CDS             42576..42917
                     /gene="fliJ"
                     /locus_tag="KLH46_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            42914..44041
                     /gene="fliK"
                     /locus_tag="KLH46_000998"
     CDS             42914..44041
                     /gene="fliK"
                     /locus_tag="KLH46_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBE0377775.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            44146..44610
                     /gene="fliL"
                     /locus_tag="KLH46_000999"
     CDS             44146..44610
                     /gene="fliL"
                     /locus_tag="KLH46_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBE0377776.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            44615..45619
                     /gene="fliM"
                     /locus_tag="KLH46_001000"
     CDS             44615..45619
                     /gene="fliM"
                     /locus_tag="KLH46_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBE0377777.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            45616..46029
                     /gene="fliN"
                     /locus_tag="KLH46_001001"
     CDS             45616..46029
                     /gene="fliN"
                     /locus_tag="KLH46_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBE0377778.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            46032..46397
                     /gene="fliO"
                     /locus_tag="KLH46_001002"
     CDS             46032..46397
                     /gene="fliO"
                     /locus_tag="KLH46_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBE0377779.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            46397..47134
                     /gene="fliP"
                     /locus_tag="KLH46_001003"
     CDS             46397..47134
                     /gene="fliP"
                     /locus_tag="KLH46_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBE0377780.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            47144..47413
                     /gene="fliQ"
                     /locus_tag="KLH46_001004"
     CDS             47144..47413
                     /gene="fliQ"
                     /locus_tag="KLH46_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBE0377781.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            47422..48207
                     /gene="fliR"
                     /locus_tag="KLH46_001005"
     CDS             47422..48207
                     /gene="fliR"
                     /locus_tag="KLH46_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBE0377782.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            48492..49115
                     /gene="rcsA"
                     /locus_tag="KLH46_001006"
     CDS             48492..49115
                     /gene="rcsA"
                     /locus_tag="KLH46_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBE0377783.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGNILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(49159..49347)
                     /gene="dsrB"
                     /locus_tag="KLH46_001007"
     CDS             complement(49159..49347)
                     /gene="dsrB"
                     /locus_tag="KLH46_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBE0377784.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            49510..49737
                     /gene="yodD"
                     /locus_tag="KLH46_001008"
     CDS             49510..49737
                     /gene="yodD"
                     /locus_tag="KLH46_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBE0377785.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            50035..50850
                     /locus_tag="KLH46_001009"
                     /pseudo
     CDS             50035..50850
                     /locus_tag="KLH46_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(50847..52541)
                     /gene="yedQ"
                     /locus_tag="KLH46_001010"
                     /pseudo
     CDS             complement(50847..52541)
                     /gene="yedQ"
                     /locus_tag="KLH46_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(52712..52894)
                     /locus_tag="KLH46_001011"
     CDS             complement(52712..52894)
                     /locus_tag="KLH46_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBE0377786.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(52973..53890)
                     /locus_tag="KLH46_001012"
     CDS             complement(52973..53890)
                     /locus_tag="KLH46_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBE0377787.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            54063..54983
                     /gene="yedA"
                     /locus_tag="KLH46_001013"
     CDS             54063..54983
                     /gene="yedA"
                     /locus_tag="KLH46_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBE0377788.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(54972..55442)
                     /gene="vsr"
                     /locus_tag="KLH46_001014"
     CDS             complement(54972..55442)
                     /gene="vsr"
                     /locus_tag="KLH46_001014"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBE0377789.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(55423..56841)
                     /gene="dcm"
                     /locus_tag="KLH46_001015"
     CDS             complement(55423..56841)
                     /gene="dcm"
                     /locus_tag="KLH46_001015"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBE0377790.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(56908..57448)
                     /locus_tag="KLH46_001016"
                     /pseudo
     CDS             complement(56908..57448)
                     /locus_tag="KLH46_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(57488..57745)
                     /locus_tag="KLH46_001017"
     CDS             complement(57488..57745)
                     /locus_tag="KLH46_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377791.1"
                     /translation="MIRGDSLWAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLG
                     STAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            complement(57746..>58080)
                     /locus_tag="KLH46_001018"
     CDS             complement(57746..>58080)
                     /locus_tag="KLH46_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBE0377792.1"
                     /translation="RNPEKQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDN
                     AVAESFFQLLKRERIKKKIYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQ
                     YYQRLGSV"
BASE COUNT        14601 a        14867 c        13968 g        14644 t
ORIGIN      
        1 attagcatta gaatagttgc gataagctgc aataagcaga gccacctttt tggtttaata
       61 agtccttaca aatagaaggg ggtctttaca cttatctaag atttttccta aatcgacgca
      121 actgtactcg tcactacacg cacatacaac ggaggggggc tgcgattttc aataatgtgt
      181 gatgcagatc acacaaaaca ctcaattact taacataaat gtataagtca tacttttgtt
      241 ttgggtgtat ttcaatctgt taaaaagttt ttcgctacgc tagcaagcaa aaatgaaaca
      301 ggaataatcg aaatgggatg ttgcgcacgg ttaaaataac tcaccgtaaa taatcatctg
      361 ctataaataa tcactttcat gcaataccag ataagctatt tttaaacaga cacttaccgc
      421 acaacaaact aattaaatac aaagaaaggc aacaatcaac ttaatgttaa taaaatgttt
      481 ttcatatatt aaccacttgt ttgatgcggg gaatcagtac attattttct tataattaca
      541 gttgaaatca tatgatcacc gagagtgatt gattaattgt ctttactaat aatcagacta
      601 atatttacct gtttgactga gttgggattg cgtcgtttct ccattaggag taaagcttta
      661 atgtcacctg aagttcacag cagaataagg aacaggttat gagctatagc aatattcttg
      721 tcgctgttgc ggttacaccg gaaagtcagc aactgctggc aaaagcggta tctatcgcca
      781 ggccagtaaa gggacacatc agtttaatta ctctcgcttc caacccggaa atgtacaatc
      841 aattagccgc gccgatgctt gaagatttac gcagcgtgat gcaagaagaa acgcaaagct
      901 ttcttgataa gttaattcag gatgcggggt atcccgttga caagacgttt attgcctacg
      961 gtgagttaag cgaacatatt ctggaagtat gtcacaaaca tcatttcaat ttggtgattt
     1021 gcggtaatca caatcacagt ttcttttcgc gagcatcctg ctcggcgaaa agagtgattg
     1081 cctcaagtga ggtcgatgtg ctgttagttc cacttacggg agattaaccg ctcctacgca
     1141 agctttggaa aggtagcaac tttatcgcgc tgctggcttt ccgcgcttcg cggaactatc
     1201 tgctttaggt cgctaatgaa gcactcctgc cagtggttaa tatcgttttt cacgataacg
     1261 tccagcattt ctgcatgacg ggaaatacgt tccaccagcg acatagtcaa tgcacgatcc
     1321 agcgcagctg caacttcgtc acgatcgtag gggttaacaa ttaacgccga cgttaactcg
     1381 tttgccgctc ccgcaaattg cgaaagaaca agaacgcccg ggttggctgg gtcctgagca
     1441 gcaacatact cttttgctac caggttcatc ccgtcacgca gtggcgtcac taagcccacg
     1501 tcagagtagc ggaatatttt catcagtaat ttacggtcaa aatgctgatt caaataataa
     1561 agcggcgtcc agcctaattg cccgtattta ccattaattc gtccagcttc attttcgagc
     1621 tgatgacgaa tatcctggta ggcttgcaca tcaccacgcg acgttggtgc aatctgggta
     1681 taacgaattt taccatgatg ctgcggatat ttttccagca atgcctcata ggcgagaaaa
     1741 cgctccggca aacctttgga ataatccagc cgttcgacag aaaagatatt ttgtacgttt
     1801 ttcaattccg ctttaagttg cgccagtttt ggcggcagtg gcccggcagc ctgtttggct
     1861 atttctttcg gttcaatgcc gatcgggtag acttctgttc gaaatgcttt tccccaggct
     1921 gtatggcttt ttgcgctacg tgtcgtgacg cgggtcaggt tagaaaggca atccaggaac
     1981 gccagacgat cgttttctgt ctgtaaaccc agcaaatcat aatcacaaag ctgttcaagc
     2041 aaggtgtcat atgtcggcag cgcgttgaaa atttccggtg tcgggaaagg aatatgcaag
     2101 aaaaaaccaa tgcgattatt caccccacgt ttgcgtaatt catgcgcaaa gggcaacagg
     2161 tgataatcgt ggatccagat aatgtcatcg tcttgcaaca gcggcagtaa tttatctgcc
     2221 agcaacgcat ttacgcgtag atagccgtcc caggcaggac gctgaaattg caccagatcg
     2281 agccgataat gaaaagcggg ccagagaacg gcattggaga attggttgta gtattcgtca
     2341 aggtcctgtt cgctgaggtt aaaagaggcc cacgtaatgt tacctttttt agcggctgat
     2401 cctcattccc tgtttcacca ctccagccaa accacagtcc gcctgcggct ttcagtgccc
     2461 ccagtatgcc aacggcaagg ccaccggcac tggcggcgtg ctcgtctggt ggtgcaatcc
     2521 ggttagatac tacgactaaa cgactcatag tcatcactcc tgttattttc tcttttttgt
     2581 tgtaatgcgg cggttatcat ttcaagccag ctccagacat ccggcacacc cgccagtcgc
     2641 catgatgcct gagttgcacc tgtgccaatt tttactgaca ttccgcccag tcggttaacg
     2701 actgcgaagc cagattcatc ggttaaatca tcgcccagaa atacgggcgt tcgcccgata
     2761 aaaggagctt cctgcataaa ggctgcaatc gcttcacctt tactggtacc tctcggtttg
     2821 atctcgacaa cacactttcc ctgctgtaac gccatttgtg gccagatctg agtaatacgt
     2881 tgcgctaatg tcattaatgc gtcttcatgc tgcggagcct gacgataatg cagcgcaaaa
     2941 gccatccctt tcgcctccag ctccgcgccg ggatactgag cgatgaccgt atgcagttgc
     3001 acgctaatat cacgcgcaat cgcatccggc agatgaacga tatgtgtttt accattgatg
     3061 tcacggcgct ccgccccatg cacgcccgct aacgggaagc gataaggttt tgccagtgcg
     3121 tcaagctcca ccattgagcg ccctgatatc aatgccaatg caccatcact tgcggttgcc
     3181 agtagctgta gtccttgcag aatattgtca ggcacgacga cctgatcggg atgcggtttg
     3241 atttccgcca gcgttccatc aagatcaaaa aaccaggcat atttcgcgga tagttcaggg
     3301 gtttcggtta acggttctgt cacccggctc tcctccttct cttttcttat tgtgttgcgc
     3361 agaccgcgct ttacagacat gtaagtatag acagtgtgac ggaggtcgcc atttgcaacg
     3421 aagaacagcc aaccgttgtg gcggctggct gtggtgggaa aaaacgttaa attgttgtgg
     3481 aaaaaagcat cagacagtgc gcttcgcttt ttgcttgtaa cggtcgaaga tcaccgctgc
     3541 cagcaggatt aagccgcgaa ccacgtactg cgcgaaagga gaaatattaa gcaggttcat
     3601 ggcgttttcc acggtgccta aaattaagat acccgccacc acatatgaga tttttccgat
     3661 gccacctttc agagaaacgc cacctaaaac gcaggcggag ataacaataa gctcataacc
     3721 aatcgacgtc attggctgcc cactagtcat acgtgaagcc agaataattc cggctatcgc
     3781 agataccagg ccagagagaa caaagataat aattttggtg cgaacaaccg gtatacccgc
     3841 cagacgcgca gcctcttcgt tcccgccaat cgccagggta ttacgaccaa aggtggtttt
     3901 attcagcagc aaaccaaaga taatcagaca cgcgacggtg agccagattg gcgcaggcag
     3961 accgaaccag ttagcgtaac caagggcaaa gaagctttca tcttcgatac cgaccgcttt
     4021 accgtctgaa atgatgtacg ccagacctcg aacaatctgc atcgttgcca gtgtcgtgat
     4081 cagagcattt attttcagtt tggcgataac aaagccattg accaggccac agagaacgcc
     4141 cagcaacaaa cccgctgcca cgccaatcca caggctttca gtcaggttga taaccaccgc
     4201 cgtggtgaca cccgcacagg caattacgga ggcgacagaa aggtcaaagt caccggaagc
     4261 aaggcagaac aacatgccac acgccaccat ccccgacatg gaaattgcca ggcccaaccc
     4321 tttcatatta atgaaggtgg caaaatttgg gacaaaaatg gcacaggcga taaagagcac
     4381 cgcaaacacc accagcatgc cgtactgatc ccagatacgc ccgaagctga atgacgactt
     4441 aggtgcgcca gaccccgatg tagaaacaga agacatcata ctctccttac tcaggcaact
     4501 gcctggctga ctttaggcat cgcaaggctc agtgcctgac gctcatctgc ctgctcgtgt
     4561 aacaattcac cggcgatttc gccttcccgc atcaccacaa tccggtcggc aacgccgagg
     4621 acttcaggta agtcgctgga ggcaaacagc accgccacgc cctgcgccgc cagcgcataa
     4681 attacgttat atatttcgtg cttagcgcca acatcaatgc cgcgcgtagg ttcatccagc
     4741 aaaatgacct tcatctcttc cgataaccag cggcccagaa tggctttttg ctgatttccg
     4801 cctgagagat tcatgatcag ttgctccgcg cccggcgttt tgatgttgag cgaacgaatg
     4861 tgctgatcgg cattgttttc ttcccaactg ttgttgatta cacaaccgcc gagcacatgt
     4921 ttgcgtctgg cactgatgtt gatattgtcg cgaacggagt gcacgggaat aatgccttcc
     4981 gctttgcgag cttccgggca gagcatcatg cctgcggcaa tggcgtggct cggtttacga
     5041 atatcgatcg gctgttggtc gatataaacc tgaccggcgg tgatttgcgt cccgccaaac
     5101 aagcctttca ttaattcgct acgccccgcc cctaccagac caaacagccc aacaatttca
     5161 ccactgcgaa ccgccagact tattggcgta cgcacgccta gtgctttcac agcatcaaga
     5221 cgcaggcgct cctcgccata actacgaggt tgccagccgt agatatcgcc aatgtcgcgc
     5281 ccgaccatcg cctgcaccag cgcgtcgtgg tcaacctgct gcatatcggt aaaggttttg
     5341 acataacgtc catctttaaa gacagtgatg gcatcgctga gggcaaatat ttcttccata
     5401 cggtgagaaa cgtataagat cacccgcccc tcttttcgca gttcacgaat aacgcggaaa
     5461 agattgccga tttcgcgggc agaaagggag ctggttggct catcaaaggc gataatttta
     5521 gcgttacgcg ccagcgcttt ggcgatttca accatctgcc actgaccaat ggcgagatat
     5581 ttcagcggcg tgtccgggtc aatatccata ccaagatgtt taagttgtaa acccgcctca
     5641 taattcagca atgagcgatt cacaatgccg cctttatgcg gcagctggcc gagatagatg
     5701 ttttccgcga cggtcatttc cggcacgaga tgcagttcct ggtaaataat cgccacaccc
     5761 gcgttaagtg ctgcggtcgt gtcggaaaag gacatttcct gcccattaat cactacagaa
     5821 cccgtggttg gcgcatagtt gccgctgagg atttttaaga gagttgattt tcctgcgcca
     5881 ttttcaccca tcaacgcatg aacctgaccg gcatagcagt caaaactaat atccgtcagc
     5941 gccttaacgc cgggaaacgt tttaccgatg ccgcgaaatg agagatacgg ggtagactgt
     6001 tgcataacgt ctccgtgaat cactggtcgt tcacaccccc tctgcatcat gcagagggga
     6061 atttttccgg caaattactt accgcctaaa ccttttttct ccagttcttc tttaaagttg
     6121 tcacgcgtga tcagtaccac gtctgtaact tcggtaaatt ttggcgtttc aacgtctttt
     6181 gctacccagt tgtaaagcat ttcgctggat ttatagccat gtacgtccgg gcttggcagc
     6241 agggaaccgt agaagccggt tgcctgtgct ttagacagtt cgcttactgc atccacaccg
     6301 ttaataccaa taccgatgat atcggctgct ttaaagccct gaccttcggt tgcacgtacg
     6361 ccgccaagta cggtgctgtc gttcataccg acgatcagcc aatgtttaac ttccggatgt
     6421 tgaaccagca ttgagttggc agcgtcaaat gcccccggga tgtcgttaga tttggtaggt
     6481 acctgataaa tttgtttttc cgggaatcct gcagctttca gtgcgtccat agaacccgtt
     6541 gtacggcggc gggcggtatc cagttcgttg gcggtaatcg ccatcactgc gctttctttg
     6601 acatcccagc cacgtttctg catctcttta tacagttcct agccctgacg ttcgccaatt
     6661 ttagtcgccg ccatcatcac cagaggaacg gtatccattg gcttaccttt ggcgttaaca
     6721 aactggtcat ccactgcaat gactttcata tcgtagccac gcgctttcgc gacgatggca
     6781 gagccgagtt tcgggtccgg agtacaaata acgaaacctt ttgcgccact ggctgccagg
     6841 ctgtcgatcg cgttcaatgt tttttcgcca tccggcacgg caatcttaat aacctcaaac
     6901 cctaaatcct tcccggcttt atcggcaaac ttccattcgg tctggaacca cggctcttcc
     6961 ggttgcttca ccagaaaacc gagcttcagg ttctccgcca tagcggattg tgacataacg
     7021 gctgccagac caatggctgc cagggcttta gtaaatttgt gcatggttct ctccagcttt
     7081 agtgtcgttt tgtgtagtgc aaaaacgaat gacattcgtt aaattaatcg gaaaacaaag
     7141 cattaccttt tatctaaaag ataagtgacc gtgttgacat agttttagcg agaaattaat
     7201 tctccatagg agagcaatat cacatcgcag aattacagtg agaacgtgca taaatttagc
     7261 gggaaaagac ataaggaaaa gccaatttgt cagacaaatt gttgaatgca cagcagatta
     7321 atccataaga ttagcctgga aatccttgtt gtcttcggta cccattcggg atgtcttctt
     7381 tttaaccagt caataggccg cattacctgg cgttgagttt ttgaaatggt gtaataacca
     7441 caactcaaag atgtggaaaa tgcgcgtcat tcatttcgcc attaattatc actgtgctca
     7501 ttaattaaca gaacacgtat aatgagagcc atctcgcaaa aatgaaaaaa cgttttataa
     7561 aatcatcact tcatcatgaa ttcaaattca tcgattaata tcaacaagat acaaaaagca
     7621 ctatcattaa aattcattgc atttacattg atttcaccaa tgaaatgtaa aaatatataa
     7681 acttgatgat ttaagcattt tcttataacc gttcagacgt tattcttatt tcagatcatc
     7741 gtcagaattg actccacgat cacatttcgg accggcagaa aggaattatt ctgcaaacag
     7801 taattatggt gttttgattt atcttgcacc tctccacttc tggatataag gatattaggt
     7861 atggcaaccg ctggaatgct tctcaaactc aactctcaaa tgaaccgcga gttttacgca
     7921 tccaatctct accttcacct gagtaactgg tgttctgaac agagtctgaa cggcaccgcc
     7981 actttccttc gcgcccaggc acagagtaat gtgacccaaa tgatgcgcat gtttaacttt
     8041 atgaagagtg tcggcgctac ccccatcgtt aaagccattg atgttccagg tgaaaaactg
     8101 aactctctgg aagaactgtt ccaaaaaacg atggaagaat acgagcaacg ttcaagcact
     8161 ctggcgcagt tagccgatga agcgaaagaa ctgaatgatg attcaaccgt caatttcctg
     8221 cgcgatctgg aaaaagaaca gcagcatgat ggcctgttgc tgcaaaccat tcttgatgaa
     8281 gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga ccgaccaaca tgttctgaat
     8341 gtcgtgtcac accagctgca ttgatcatac atcggcgcta atacattgcg ccgatgaagt
     8401 ttttgagaaa ccgctgcctc atctgtttga agcagcggtt tttttaatgg gattcaccct
     8461 gtggggtaaa cttgagttca atcagcgcga tggctttttg gattgcccgc atggtgaccg
     8521 ggtctgcggc agcgggatga tgagtaaagt cgatattctt cagctgactg gacatttttt
     8581 cacgaacttc aacgggcgcg attacatcga gaacatccag aatttgtttg ataaccaact
     8641 ggcaagctac cacatcagaa accagttcct gatcggcatt cagcggctgg gacatcgtaa
     8701 actcctgata gcattttgaa agccgttata gtagcggctt cacatcttca gcgatagtca
     8761 catccaccgt catcaggaca caaaaaaacc tgccggagca ggttttttgt tatcggaaca
     8821 tattgcctgg cggtacgtct ttgaacgtct tgcaatagtt attgaacata cttttcagga
     8881 ttttgcgcag tttcatcgcg gcactccgac catttgttat acaggtgtta ttgtctttgc
     8941 gcttataata tgacaaccat cacaaaaatc aatctttatg tgatacaaat cacataaata
     9001 cccctttaat gttataaaaa tgataatcaa aaaacagccc ccctatttct gacacctaca
     9061 gatggcaaga aatagcgcct gccaggcgtc ttttccggcc attgtcgcag cgctgtaacg
     9121 cgtaaaatag tgctttctct tactcttctg gctggaccat gagacttctg attctgactc
     9181 tttcattaat aacgctggcg gggtgtacgg ttactcgcca ggcccatgtg agcgaagttg
     9241 acgcggcaac aggtattgta cggttggttt atgaccaggc ttttttgcag catgcccata
     9301 ccgatcgtta tgtcagtcgc gggatcgccg atcgcgcatg ccagcaggaa ggctataccc
     9361 acgcgatccc ctttggtcag ccagtaggca actgtagcct ttttgcgggt tccttatgtt
     9421 tgaatacgga attcactttg tcatatcagt gccatcattc cgccttccct gtctttctct
     9481 aaatatttta atcagcgagg ggatcttcgc tgattaaaga aatagcggta atgcgtttta
     9541 ttcccattcg tatttttaat aattgaagtt tatattttac cttttgcaaa taataaaata
     9601 acaaattata gtgacgccac agcaacaaat ataacctttg tggagcacta tcatgctgaa
     9661 accagaaatg attgaaaaac ttaatgagca gatgaacctg gaactgtact cttcactgct
     9721 ttatcagcaa atgagcgcct ggtgcagcta tcataccttc gaaggtgctg ccgcgttcct
     9781 gcgccgtcac gcccaggaag agatgacgca tatgcagcgt ctgtttgatt acctgactga
     9841 taccggcaat ttaccgcgta ttaataccgt tgaatctccg tttgctgaat attcctcact
     9901 tgatgaatta ttccaggaaa cctataaaca cgaacaatta atcacccaga aaattaacga
     9961 actggctcat gctgcaatga ccaatcagga ctacccaaca tttaatttcc tgcagtggta
    10021 tgtttctgag cagcatgaag aagagaaact gttcaaatcg attattgata aattaagcct
    10081 ggcaggcaaa agcggcgaag gtctgtattt tatcgacaaa gaactctcta ccctcgacac
    10141 acaaaactaa tgctctccgg cggcaggttt ctgccgccgt ttcaaatcag tgacggcaaa
    10201 tcttactctg atcggtcgaa aaaccttctt ctgaaggtat aaatttgcct tttgctgcca
    10261 gaaacgctac cagctctccc gctgtcaccc cttctgccga gcacgtgtga aaacgtgcct
    10321 gttcaccaaa acgcgcttta atcgcagctt ccagactggc atgcgtatat tgctcgcctg
    10381 attcaatcat catatttaac acttcatgac cgtgaataga gtccatcgtt cctcctcaaa
    10441 aaaagcctag cgtagcgatt accgcttatg aagactttgc gccagcgcag gactgaatgc
    10501 ttttattgta catttatatt tacacaatat gtaacgtcgg tttgacgaag cagccgttat
    10561 gccttaacct gcgccgcaga tatcactcat aaagatcgtc aggacagaag aaagcgtgaa
    10621 aaacagaacc ctgggaagtg tttttatcgt ggcgggaacc acaattggcg caggcatgct
    10681 ggcaatgccg ctggctgcgg ccggtgttgg ttttagcgtt acgttaatct tgttgattgg
    10741 gctttgggcg ttgatgtgct acacggcgct attactgctg gaggtgtacc agcatgttcc
    10801 ggcagatacc ggtctgggca cgctggcaaa acgctatctg ggacgctacg gtcaatggct
    10861 gacgggcttc agtatgatgt tcttaatgta tgctctgact gcagcataca tcagcggtgc
    10921 cggtgaattg ttggcctcca gcatcagcga ctggacaggt atttcaatgt cggcaaccgc
    10981 tggcgtgctg ttgttcactt ttgttgccgg tggcgtggtt tgtgttggaa catcgctggt
    11041 cgatttattt aaccgttttc tgttcagcgc caaaattatt tttctggtgg tgatgctggt
    11101 attgctgcta ccgcatattc acaaagtgaa tcttttaacc ctgccgttgc aacaggggct
    11161 ggcactgtct gcaatcccgg tgatttttac gtcgtttggt tttcacggta gcgtgccgag
    11221 tattgtcagc tatatggatg gcaacattcg taagctacgc tgggtgttta taatcggtag
    11281 tgccatcccc ctggtggcat atattttctg gcaggtggcg acgcttggca gcattgattc
    11341 aacaaccttt atgggattgc tggctaatca tgctggatta aacgggctgt tacaggcgtt
    11401 acgcgaaatg gtggcctctc cgcatgttga gctggcggtg catttatttg ctgatttagc
    11461 cctcgccacg tcatttctcg gcgttgcgtt aggcttattt gattatctgg ctgatttgtt
    11521 tcagcgttca aataccgttg gtggacgatt gcaaactggt gcaattacct ttctgccgcc
    11581 gttggcgttt gcactgtttt atccacgagg atttgtgatg gcgctgggtt acgccggtgt
    11641 ggcgctggcg gtactggcat tgattatccc ttcactattg acctggcaaa gcagaaagca
    11701 caatcctcag gcgggttacc gggtcaaagg tggtcgtccg gcgctggtgg tggtgtttct
    11761 ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg gcaggcttgt taccagaagt
    11821 ggggtgatca gatagcctca aattccttat tgggtgccag aattaacgct gacacccaat
    11881 gtgacctctt aatgcaggca gcactgctta aatttcttac cactgccgca cggacaagga
    11941 tcgttacgcc ccggtttctc ttctgctttg atcggttgct gaacagcttt ttcctgcgga
    12001 tgcgccatcc agtatgcatg taaatcaagc gccgccagtc gaatggcatc tacgctctct
    12061 tcaaacgctt ctggcgacat tttctctacc cgctcgaagt tttcctcagt accgtgcagc
    12121 gcaatcgcct ccagcgctgg ttttaaagaa tcgggcaacg ttgaccagtc agaaagtgcc
    12181 acgccccgca tatagccaaa gcaccactcc tcaacaatcg tcagctcgct gccatcaact
    12241 tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt cgttcagacg ctctgcggta
    12301 tcggccatat gttgaaaagc caggttcata aagcgcgtca tctctttctc tgacgcccaa
    12361 cgcggcacat agtcagcccc accccacacg gcaaccagcc actgttctgg ttcaatctct
    12421 tgcggagaac tcaacaccgc cgtcaataaa ccgtccagct ccgccacatc aaggatggcg
    12481 tggtcagtgt tgtatttggt cagaatatcg tccagccatt ccaactcact ttcgtttaac
    12541 ggtcccgttt tcatacgctt ttccttgtgg atctcaactc gccagcacct atcttacatg
    12601 ccggtccgta tcagagatac tttttgagtg gctttgccgg tgattaaaaa ttaaggaggg
    12661 tgtaacgaca agttgcaggc acaaaaaaac cacccgaagg tggtttcacg acactgctta
    12721 ttactttgat tttattctta tctttcccat ggtacccgga gcgggacttg aacccgcaca
    12781 gcgcgaacgc cgagggattt taaatccctt gtgtctaccg attccaccat ccgggctcgg
    12841 gaagaaagtg gaggcgcgtt ccggagtcga accggactag acggatttgc aatccgctac
    12901 ataaccgctt tgttaacgcg ccaaattctt caggcctttc agccagacat ccgcttgacg
    12961 ccgatgtctt ttaaactgga gcgggaaacg agactcgaac tcgagacccc gaccttggca
    13021 aggtcgtgct ctaccaactg agctattccc gcattcatca agcaatcagt taatcacttg
    13081 attttattat cgtctggcaa tcagtgccgc cgttcgatgc gttgcattct acttacctgg
    13141 cgcgatgagt caacgatatt tttcaccact tttgatcgtt tgctgaaaat tacgccgaaa
    13201 cgatcactga tcaagcaaat ctgcacgcgc agcgctcaaa tattgcaaca ttgaccacag
    13261 agtcagtacc gcagccacaa agaaaagtgc aataccgacg tactcaaccc aaatgttcgg
    13321 acgccacagc agccaggcca acgccaccat ctgggcagtg gttttcactt tcccaatcca
    13381 ggagacggcc acgctactgc gtttacccaa ctccgccatc cattcgcgta gcgcagaaat
    13441 aataatttca cgggcgatca tcgttgccgc cggtaatgtc acccaccagc tgtgataatg
    13501 ctcggttacc agcaccatgg cgatagccac gagaacttta tctgccacag ggtcaaggaa
    13561 agcaccaaac cgggtactct ggttccagcg gcgtgccaga aaaccatcga accagtcagt
    13621 caccgccgcg acgcagaaaa tgagcgcggc ggcaaacggc gaccaggtga caggcagata
    13681 aaagaccaat acaaagaatg ggataaggat gacacggaac agtgtaagca acgtagggat
    13741 attaaattgc ataatgacgg gtaactatct gttgtcagta agattacccc tatgttgcta
    13801 cagagacatc aatgtttcaa cgaccagaag atcttttctg ccagaccttg cgaaataccc
    13861 ggcacttttg caatttcctc gacgctggcg ttacgtaaac cttgcaaacc gcccatatat
    13921 ttcaacaaca tttgccgacg ttttggcccg acgccttcaa tggtttccag ggaactggta
    13981 tttttgacct tcgcccgttt tttacggtgc ccgccaatcg cgtgatcatg tgattcatcg
    14041 cgaatatgct ggataacatg cagcgcgggt gaatctggcg gcaaactaaa tccctcacct
    14101 tccggctcaa agaacagcgt ttccagtcca gccttacgat ctgctccttt ggcaacgcca
    14161 agtagcagcg gatgattttt atcccatgag acatccagtt cggcgaagac atttttcgcc
    14221 tgcgcaagct ggcctttgcc gccgtcgata aggatcacat ccgggatctt actgtcgtca
    14281 atggctttac cataacgccg acgcagcacc tgattcatcg ccgcataatc atcgcccggc
    14341 gtgatgcctg taatgttata gcgccgatac tccgcacgca gcgggccgtt agcatcaaac
    14401 accacacagg aagcgacggt ttgttcgccc atggtatggc tgatgtcaaa gcactccatc
    14461 cgcttcactt ccggcaattt caacacgctg gcaagcgcgg tcagtcgctg gtgaacggta
    14521 gattgctgcg aaagtttgct ggttaaggcc gtcgccgcat tggtgcgcgc gagtttcaga
    14581 taacgcgccc tatcgccgcg aggtttggtt tgaacattaa tcttgcgtcc cgccagttct
    14641 gaaagggaat cggcgagcag cgttttatcg ctaagattaa aatcgagcag gatctcaccc
    14701 ggtaaggtgc gcatctggct gccttgtaaa tagaactgac ctacgaaggt ttctaccacc
    14761 tcgctcagtt ccgtaccgcc aggcactttc gggaaatagc tgcggctgcc gagcactttg
    14821 ccctgacgaa tgaacaatac gtggacacaa gccatgcccg catcgaacgc cacaccaata
    14881 acgtcaaggt cgtcgccggt attggaaaca aactgttttt cggtgacgcg tcgcaccgcc
    14941 tgaatttggt cgcgaataca tgccgcttct tcaaactcca gattctggct ggcagtttcc
    15001 atacggctaa tgagttgcgt aagcacctga tcatctttgc cagacaaaaa caggcgcaca
    15061 tactcgacct gctgagcgta ttcttcttca ctcaccagtc cttcaacgca cggtcccaga
    15121 cagcgcccta tctggtattg cagacacgga cgcgagcgat tgcgataaac actattttcg
    15181 cactggcgaa tggggaaaat cttttgcagt agcgccagtg tttcacgtac ggcatagcca
    15241 ttcgggaacg ggccgaaata ttcacctttg gcatgctttg caccacgatg catcgccaga
    15301 cgcgggtggg tatcgccact caggaagata aaaggataag atttatcatc gcgtagcaaa
    15361 acgttgtaac gcggctgata gagtttgatg tagttgtgtt ccagcaacag cgcttcggtt
    15421 tctgtatgcg taaccgttac atcaatttgc tggatctggg cgaccagcgc ttcggttttg
    15481 cgcgaagcga ggttgctgcg gaaatagctg gaaagccgtt ttttcaggtc tttcgctttg
    15541 ccgacataga taaccgtacc accagcatcg tacatgcgat aaacgcctgg ctggctggtt
    15601 acggttttta aaaacgcttt tgcgtcaaac tgatcactca ctgacttgat aatgtctccg
    15661 cattacacag accatggcga attgccaggt gagtcagctc aacatcgcca tgaatgttta
    15721 gtttactgaa catacgatag cggtagctgt tcaccgtttt cggactgaga ttgagctgtt
    15781 ctgagatctc attgaccttc tggcccttag tgatcatcag cataatctgc aattcacgtt
    15841 cagacaaact ggcaaatggg ctttctgttt tttctggttc gatctggctt aacgccattt
    15901 gttgagcgat atcagaagca atgtaacgct gccctgaata gacagaacga atcgcactca
    15961 cgacttcctg cggagccgcg cctttgctga ggtagccagc agcacctgcc tgcatgactt
    16021 tcgctggtaa agggttttct gtatggaccg taagcatgat gattttaaca tcagctgtgg
    16081 aacgcgcgat tttacgcgtc gcctcaagac cgccaatgcc cggcatactc atgtccatta
    16141 gcaccacgtc aacggcattt gtccggcacc acttaacggc gtcttcaccg cacgatgcct
    16201 ctccggcgac ttttataccc ttaatatctt ccagaatgcg tcgtatccct gcgcgcacca
    16261 gttcgtggtc atcaacaagt agaacgttga tcaaaggaat atctccagaa atagggataa
    16321 cgctactgat agttagtcat tcgtatatta gcggttttta ttgcaacttt gaaacgttaa
    16381 aaatggtggg tattcgattt ttctctcgtt tttgtcaatt cgattgtcca cagttatggt
    16441 cacgcccgtc accaacggct taccggatcg tttcacagta agccctacga tgaaatgtga
    16501 caaaaattga ctttattcag caaaaatgaa aatcagcccg ctgaatatgt aacattaatt
    16561 aacccgaaca atacgggcaa aaaacattca tgataaaaat atttattggt cattatatta
    16621 acgtatttta tagcactgcc gatatcacgc tcaaaaaaca accactgcta tttttagcaa
    16681 agcttatggt atactccgcc gccttaacat ttttcaccac aaattttcat tgcaacatga
    16741 cgaggaaaat aaatgagtac gcctgatttt tctactgccg agaataatca agaactggca
    16801 aatgaagtct cctgcctgaa agcgatgctg acgctgatgc tgcaggcgat gggacaagct
    16861 gacgcgggcc gcgtgatgtt aaagatggaa aaacagcttg cgctgatcga agacgaaacc
    16921 caggctgcag tattttccaa aacggttaag caaattaaac aagcctaccg tcagtaatat
    16981 aaaaccggct gagagcgtgc ctttcagccg gttattgaca aaatcatcgc tatctctgct
    17041 gatgtcttcc cttccgccgt ccatttcaga atttcttttt cgcgcttgct gaaattcatc
    17101 tctggcgtca tcactatttc atcatttaaa cgcatcagag ccatcagact ttcgcgcacc
    17161 agtaactgca tttttaattg cagttcatca ctaagaatgg gtatttcgcg cgtgctgcaa
    17221 cgggaaaagg acaaaaagcc cagcgcccga tttggcagca ttaaatactg agtgacaccg
    17281 cggcgtaaac catgtgcgtg cgcggcttcc cataatggct gtgcttcgct gaataagtca
    17341 tcattccaca ttaaatggcc ctgactaaag ttttcagggt tcagcacagg atcaattgcg
    17401 agaaaatttt ttgcctgata ataactaacc cacgactcag ggtaattggt gtagaaagcc
    17461 actttaggtc gcgtgaatgg taccgggtgg cgaacacata acgaatagta atcgtactcc
    17521 agctgctgag cctgaagctc tatttcatgg taggcctctt ctgcggcctc catcctctga
    17581 aaacgcaaca gcatcgtgcg acgccagctg aaaaaatccg tatcctgcat agtaaaccgc
    17641 aacgcccctg agagtgagta tcatttataa tgataattca aactatcaca taaataagat
    17701 ttatatataa tttatattat tcaggcaatg aattactttt gcaagccatc gcattctctt
    17761 atgttattaa tgagttatgc tgatttgtta agcagtttta tcacgcttga aatggcgtcc
    17821 cgccccgaca ggtgaatcgt cggggctgat tttttcttat tattgcagca gaagcttctc
    17881 gaggaactgg cgggtgcgag gctgctgggg atcggcaaat aacgcttttg ctgccccctg
    17941 ctcgactatc cgcccctggt ccataaagat cgcccggtcc gcaacatccc gggcaaagct
    18001 catttcgtgt gtcacaatca ccatcgtgcg cttttcctgc gccagctggc ggatggtgtt
    18061 caggacttcg cccaccagct ctggatccag cgccgacgtt ggctcgtcaa acaaaatcac
    18121 ctcaggacgc attgccagtg cacgcgcaat cgcaacacgc tgctgttgac cgccagacaa
    18181 acgacgtgga tagctggttt ctttaccggc cagcccaact ttcgccagca gctcgcgagc
    18241 gcgcgccgtg gcctcttctt tcggttcgcc tttgacgatc accggccctt caataatgtt
    18301 ttccagcacc gtacgatgcg gaaacaaatt aaagttctgg aagacaaacc cgacgtgctg
    18361 acgcaactgg cgaatcagcg atttttgctg acttaatgaa cgtgcagtat caatagtgat
    18421 atcgccaacg gtgatcgttc ccgcttccgg ttgttccagc agatttatgc tacgtagcaa
    18481 tgtggttttg ccggaaccac tcggaccgat aattgccacc acttcgccag gctttacctc
    18541 gaggtcgata ccgtgcagca ccgtctgacc gtggaatttt ttcaccaggt tcttaacttc
    18601 aatggcactc attttggttc tctctcctgg cgattaagtt gattctcaaa atggttctgc
    18661 aacgtcgata acacagtcgc catgatccag tagatgagcg aagccgccag gtacatggtg
    18721 aacacctcca gcgtgcgcga ggtaatcaac tgcgcctgac ggaacagctc cggcacctgg
    18781 attgtcgcgg ccagcgacgt atcttttacc agactgataa agctgttcga cagcggcggc
    18841 aacgccaccc gcgccgcctg tggcaaaatc gcgcgacgca tggtctgcca cggcgtcata
    18901 ccaatactgg cagcggcttc ccactgacct ttatcaatag aagaaatggc ggcgcgcagc
    18961 gtttcggcgg cataggcggc agtgttcagc gacagaccaa tcattgctga cggaatggga
    19021 tctaattcga taccaaactg cggcaggccg taatagatca taaacagttg tgcgatgagc
    19081 ggcgtaccac gaaaaataga gatataaaaa cgcgccagcc agcgcacggg ccagattggc
    19141 gacaggcgca tcagcgcgag aataaacccg agtagtaagc caaaaaacat gccgccgata
    19201 ctgagttgca gggtataccc agcccctttc aacaggaacg gcaaggaatc aataaccagt
    19261 tgtatacttt cttgcatgag cgttcccgga gctaaacgtg gggatgatag gcgaacagcg
    19321 caggcgcgcc gccggtatga ataaacagaa tcggcccttc atctttgaag cgtttctgac
    19381 tgataccgtc aatcagcccc gccatcgctt ttccggtata cacaggatca agcagaatgc
    19441 cttccagacg cgccagcaat ttcactgctt ccatgccttc gtcgttcggc acgccgtagc
    19501 caggtgcaaa atagtcatcc cagagtaaaa tttccgctga tgcggtcagc tccagttctt
    19561 tcgcaatcgc ctgttgtagg ttaaccactt tcggcaattg atcggcaacg gaacgcgaca
    19621 cggtcacgcc aatcagttcg ctttcaggca tcaggtgttc cagcccaaca gccagtccgg
    19681 cgtgagttcc ggcactgccc gatgcgacta ccaccgacga aatattaacc gccccttcac
    19741 actgttgcgc gatttccagc gcactctcca cataacctag cgcgccaaga gcattagaac
    19801 cgccaaccgg aatgacatac gggcgaaagc cttgtgcttc gactcgcgtc gccagttctt
    19861 ccagttgggc attgggatcg gtcagtgcgt cgcacatttc aatctgggta ttgaacagat
    19921 ccaacaacaa acgattgccg ttggttaaat agttttctgc ggttgtgcca ataggatttt
    19981 ccagcagcgc cacgcagtgc agaccgagtt tcgccgcgac tgcggcagtc tggcgcacat
    20041 ggttagactg gatcgcgccg gcagtaatca gcgtatcggc accttcgcgc agggcatctg
    20101 ccgcgagaaa ttccagctta cgtaatttat tgccgcccat tgccatgggg gtgacctcat
    20161 cccgtttgat gaaaatttcc cgtccaagat aatcagaaaa gcgcggcaga tattcgagcg
    20221 gcgttggcgc gccgataaac tccaaccgtg gaaaacgggt taaattatgc agtggcataa
    20281 cagcctccga tgtgtgttgt tgtgattttc ttattatgca cgctgaaaac gcgtaaataa
    20341 aaaaggcgct agtgaaagcg cccttttttg tcattatgct gattatttgg tcacatcagc
    20401 accaaaccat ttttcggaaa gagcttgcag agtgccgtct ttttgcattt ccgcaattgc
    20461 atcattcacc gctttcagca ggtcttcatt tcctttacgc agcgccacgc cagactcctg
    20521 acgggagaat gcttcaccgg ttactgccag cgtatcgttg gttttcttca ccagatccag
    20581 cgccgccaga cgatcaacga ggatcgcatc gatacgccct acgcgcagat cctgatattt
    20641 ggtcgggtca tcatcatagg tacgcacatc gacaccctga acattctgcc gcagccactc
    20701 ttcatagttg gtgcccagac cgacacccac ttttttgcct ttcagatcag cagccgtttt
    20761 aatggtgcct tcgttacctt ttttcaccag cgcctgaata ccggaaatgg tgtaaggggt
    20821 agagaaatcg tattttttct tgcgctcgtc agaaatggtg acctgattaa tcaccacatc
    20881 aatgcgttta gagtccagcg acgccagcat accgtcccat ttggtcggtt ttagtgacgc
    20941 ctcaacgcca agatgttttg ccagctgttg ggcaaattcc acttcaaaac cggttaattt
    21001 gccgtcatcc ccctgaaaac tgaacggcgg ataagtgcct tccagcccta ccagcagcgt
    21061 gccgctctct ttgactttat taagcagacc ttcatctgca aaacttttaa cgctcatgcc
    21121 cgcaaccagc gccacggcca tcacacccat caatgcctga cgtcccagat gtgctaattt
    21181 catattcacc ccgaatgttg ttatgtctgt ttgcagtgta gagccattgt ttgtaaacac
    21241 aaaaacaact ccgctacatc ttattcttat ttaatatata tcagaagcag gcaggctgga
    21301 ggatttctgc acaagtccgg tacaggtttg gcgctgatag tgttgatact tccgtaatgc
    21361 gatgcggtaa ttgttggtgc tggtggtggg cagccacggg gcgaggattt catcaaggaa
    21421 accgtcctgc agctgatcga gggaaatatt ttgctcatgt agatgattac ccaaacggcg
    21481 cagacgaacg acatattcac gcaccgtacc tgggctcatt tcagtttgct caaaaagaaa
    21541 tttcttaaag ccgataatat cgaaaaaatc actctgcgtt ttgcaatgta aatcaccgca
    21601 aaatcggcac aatgccgccc atgattgttg ttccgtttgc cagccttttt catcaagcag
    21661 cgtgtccagg cgggaaatct cgattttatt cactattttg ccgtcgcgaa ctaaggtaat
    21721 gcgatcgagt aatttacggc aatgcgcgca atgggtctgg ctgtgtttaa agtctttaag
    21781 atagcggctt aatggccgtc ttttcaggtg ctgcaccatc attaagaact cctggtagtc
    21841 aaagttaaag tgcggcattt actgacgtta taacttaccc agtttagtgc gtaaccgttt
    21901 aatggcctgg ctgtgtaact gactgacccg cgattccccg acctccagca ccgcgccaat
    21961 ctctttgaga ttcagctctt cctggtaata gagggttaat accagttttt cgcgctccgg
    22021 caacgtttcg atggcttcca tcacccgctg gcgcagatta ctgtccagca gttgttgtag
    22081 cgggttttct cgctgatgat catcagtaac cagttcgatg ctatcgccgt gctcttcacg
    22141 ccactcatcg taggagaaga gctggctgtt attggtgtcg agcagcattt gacgataatc
    22201 ggcaatatcg atccctaaac gttccgctac ctcagtttcc gtggcgttgc ggccaagttc
    22261 ctgctccagt tgccctattg cctgtgccac ttcacgcgcg ttgcgtcgca cgctgcgcgg
    22321 cacccagtca cggctgcgaa gttcatccag catagcgcca cggatacgct gcactgcgta
    22381 agttgtaaat gccgttcctt gtagggcgtc atagcgttcg acagcattaa gtaacccaat
    22441 gccgcccgcc tgtagcagat cgtcaagttc cacgctcgcg ggcagtcgaa cctgcaggcg
    22501 caatgcttcg tgacgcacca gcgggacata acgctgccac agcgagtgtt tatccattac
    22561 accttcagcg gtatagagtg aattcacgat aaacagccct gcgttatatg agttatcggc
    22621 atgattatcc gtttctgcag ggtttttaat cggacgatta gtgggtgaaa tgaggggtta
    22681 tttgggggtt acgcaaaaag ccgaatgtaa aaaaccccgc cggtggcggg gtttgagagg
    22741 cattcagacg attaaccctg cagcagagac agaacctgct gcggtacctg gttggctttt
    22801 gccagcacgg agttaccggc ctgctggata atctgcgctt tcgacatgtt ggacacttcg
    22861 gtcgcatagt cggcgtcctg aatacgggac tgcgcttcag acaggttggt agtggtgttg
    22921 ttcaggttgg tgactgcgga atccaaacgg ttttgcaccg cacccaggga ggagcggaat
    22981 ttgtcgatgg agctgatggc ttcatccaga gctttcaggg gatcggctgt cgtagctgct
    23041 tttgtttcag catctgtcgt caatttacca tcagcatctt tataaactgc cttaccgctg
    23101 ccgttagtca ctgaaccatc atcctgtagg taaaaatcta cagtatcaga tgtggtcaac
    23161 gcctcgttgt tatcaacata aaccgaatca acaccttttg ttacagcgcc agttgtgccg
    23221 ttgacgctat aacctgcagt agtgaaatca acattgtttc ccgaggttga tacagcttta
    23281 tcgaaagctg cctgatcaac ttttgcacct gttactgaat aagtaatagt gtccggcgta
    23341 gtactattac cggtaaagga gatagaggta ccgccaatgt caatggttgt cgctttggta
    23401 ttattggctg caagtgtgga cagactagcc tcagtcaaag aagcaccaga gccgtttttc
    23461 gttaagcgcc cagttgtatc aatgtagagt ttatctccat tgcttgccgt aattttacca
    23521 tcactgctga tctgaacatc ctgtgcagaa ccatcgatag taatagtgcc cttagcggta
    23581 tcaccaactc ccggggtcaa atatttctga acatcggcag ctgaagccgt tgtggtatca
    23641 aaagagtatg acttacttgc actgtcatac ttgtaattcg ttgcactagc cgccgttccg
    23701 aagccgttat taatggttgc ctgaacagta tcaccatcac taactccagc cagcaaatca
    23761 gacgctttag cagcatcgta acccgcactc acagtatatt tgttgccgac cactgttgca
    23821 ttagcagcta ccaagtcagc tttagatgca gcagtattag ccacagcccc gccaccattc
    23881 acattaaacc cattcagccc cagcgtatca gagtcaattt tcttcagatc aatagtgata
    23941 gtctggccgt cattcgcacc aacctgaatt ttcatcgaac cgtcttttgc cagtacgttc
    24001 acgccgttga actgggtctg accggatacg cggtcaattt cgtcgagacg ggatttgatt
    24061 tcgtcctgaa tggagtccag atccgaatca gagttagtcc cggtagaagc ctgaaccgtc
    24121 agctcacgga tacgctgtaa gttgttgttg atttcggaca gcgcgccttc agtggtctgt
    24181 gcaacagaaa taccgtcgtt ggcgttacgt gcagcctgag tcaggccttt aatgttagaa
    24241 gtaaaacggt tagcaatcgc ctgacccgcg gcgtcatcct tcgcgctgtt aatacgcaag
    24301 ccagaagaca gacgctcgat agaactcgac agcgcagact ggttcttgtt gatattattt
    24361 tgagtgatca gcgagaggct gttggtatta atgacttgtg ccatgattcg ttatcctata
    24421 ttgcaagtcg ttgattacgt tttgggtttc tacccgtcgg ctcaatcgcc gtcaaccctg
    24481 ttatcgtctg tcgtaaaaca acctttagaa tttttttcac aaacagccat tttttgttag
    24541 tcgacgaaat actcttttct ctgcccctta ttcccgctat taaaaaaaac aattaaacgt
    24601 aaactttgcg caattcaggc cgataacccc ggtattcgtt ttacgtgtcg aaagataaaa
    24661 ggaaatcgca tggcaagtat ttcatcgctg ggagtcgggt caggtctgga tttaagttca
    24721 atccttgata gcctcaccgc cgcgcaaaaa gcgacgctaa cccctatttc aaatcagcaa
    24781 tcgtcgttta ccgctaaact tagcgcctac ggtacgctga aaagcgcgct gacgactttc
    24841 cagaccgcca atactgcatt gtctaaagcc gatctttttt ccgccaccag caccaccagc
    24901 agcaccaccg cgttcagtgc caccaccgcg ggtaacgcca tcgccgggaa atacaccatc
    24961 agcgtcaccc atctggcgca ggcgcaaacc ctgaccacgc gcaccaccag agatgatacg
    25021 aaaacggcga tcgccaccag cgacagcaaa ctcaccattc aacaaggcga cgacaaagat
    25081 ccgattacca ttgatatcag cgcggctaac tcatcgttaa gcgggatccg tgatgccatc
    25141 aacaacgcaa aagcgggcgt aagtgcgagc atcattaacg tgggtaacgg tgaatatcgt
    25201 ctgtcagtca catcaaatga caccggcctt gataatgcga tgacactctc ggtcagcggt
    25261 gatgatgcgc tacaaagttt tatgggctat gacgccagtg ccagcagcaa cggtatggag
    25321 gtctcggtcg ccgcccagaa tgcgcagctg acggtcaaca acgtcgccat cgagaacagc
    25381 agcaacacta tcagcgacgc gctggaaaac atcaccctga acctgaacga tgtcaccacg
    25441 ggcaaccaga cgctaaccat cactcaggac acctccaaag cgcaaacggc gattaaagac
    25501 tgggtgaatg cctataactc gctaatagat accttcagca gcctgaccaa atacaccgcc
    25561 gtagatgcgg gagctgatag ccagagttct agcaatggcg cactgctcgg cgactccacg
    25621 ctgcggacga ttcagacgca gttgaagtcg atgctgagta ataccgtcag ttcttccagc
    25681 tataaaacgc tggcgcagat tggtatcacg accgatccca gcgatggcaa actggaactg
    25741 gatgccgaca aactcaccgc tgcactgaaa aaagatgcca gcggcgtagg tgcattgatt
    25801 gttggcgatg gtaaaaaaac cggcatcaca accaccatcg gcagcaacct gaccagttgg
    25861 ctttcgacaa cgggcattat taaagccgct accgatggcg ttagtaaaac cctgaataag
    25921 ttaactaaag actacaacgc cgccagcgat cgcattgatg cgcaggtcgc gcgctacaaa
    25981 gaacaattta cccaactgga cgttttaatg acctcgttaa acagcaccag cagctactta
    26041 acgcagcagt tcgaaaacaa cagtaattcc aagtaatcat tattcatcgg gagacaggtc
    26101 atgtacgcgg caaaaggcac ccaggcctat gcacaaattg gcgtcgaaag caccgtaatg
    26161 agcgccagcc aacagcagtt ggtcaccatg ctatttgatg gagtgctgag cgcactggtt
    26221 agagcgcgac tgtttatgca ggacaacaat cagcaaggca aaggcgtctc tttgtcaaaa
    26281 gcgatcaaca tcattgagaa cggactgcgg gtgagtcttg atgaagagag caaagacgaa
    26341 ttaacccaaa acttaattgc tctttatagc tatatggtca ggcgtttgct gcaagccaat
    26401 ttacgcaacg atgtctccgc agtcgaagag gtggaagcat taatgcgtaa tattgccgat
    26461 gtctggaaag agtctttact ctccccttct tcgattcagg acccagtctg atgaacaatg
    26521 caccgcattt gtatttcgcc tggcaacaac tcgtcgaaaa aagccagctc atgttacgcc
    26581 tggcaacgga agaacaatgg gacgaactca tcgctagcga aatggcgtat gtgaatgcgg
    26641 tgcaggagat cgcacacctg actgaagagg ttgccccgtc cactacgatg caggagcagc
    26701 tccgcccgat gctgcacctg attctcgaca acgaaagcaa ggtaaagcag ttattacaga
    26761 ttcggatgga tgaactggcg aaactggtcg gtcagtcatc ggtgcaaaaa tcggtgttaa
    26821 gtgcctatgg cgatcagggc ggctttgtgc tggctccgca ggataacctc ttttgaatct
    26881 taatgagccg atggctcgcg aataatccga ttacggctac gcttctaata ttccccttga
    26941 atggagtcga agaatgcgta atcccacgct gttacaatgt tttcactggt attacccgga
    27001 aggcggtaag ctctggcctg aactggccga gcgcgcagac ggttttaatg atattggtat
    27061 caatatggtc tggttgccgc ccgcctataa aggcgcatcg ggcggctatt cggtcggcta
    27121 cgactcctat gatttatttg atctaggtga gtttgatcag aaaggcagca tccctactaa
    27181 atatggtgat aaagcacaac tgctggccgc cattgacgcc ctgaaacgta atgacattgc
    27241 ggtgctgctg gacgtggtag tcaaccacaa aatgggcgcg gatgaaaaag aagctattcg
    27301 cgtgcagcgt gtaaatgctg atgaccgtac gcaaattgac gaagaaatca ttgagtgtga
    27361 aggctggacg cgttacacct tccccgctcg tgccgggcaa tactcgcagt ttatctggga
    27421 tttcaaatgt tttagcggta tcgaccatat cgaacaccct gatgaagatg gcatttttaa
    27481 aattgttaac gactataccg gagaaggctg gaacgatcag gttgatgatg aactcggtaa
    27541 tttcgattat ctgatgggtg agaatatcga ttttcgcaat catgccgtga cggaagagat
    27601 taaatactgg gcgcgctggg tgatggaaca aacgcaatgc gacggctttc gtcttgatgc
    27661 ggtcaaacat attccggcat ggttttataa agagtggatc gaacacgtac aggaagttgc
    27721 gccaaagccg ctgtttattg tggctgaata ctggtcgcat gaagtcgata aactgcaaac
    27781 gtatattgat caggtggaag gcaaaaccat gctgtttgat gcgccgctgc agatgaaatt
    27841 ccatgaagca tcgcgcatgg gtcgcgacta cgacatgacg cagatcttca ctgggacgct
    27901 ggtggaagcc gatccttttc acgccgtaac cctcgttgcc aaccacgaca cccaaccgtt
    27961 gcaagccctc gaagcaccgg tcgaaccgtg gtttaaaccg ctggcgtatg ccttaatttt
    28021 gttgcgggaa aatggcgttc cttcggtgtt ctatccggac ctctacggtg cacattacga
    28081 agatgtcggt ggtgacgggc aaacctatcc gatagatatg ccaataatcg aacagcttga
    28141 tgagttaatt ctcgcccgtc agcgtttcgc ccacggtgta cagacattat ttttcgacca
    28201 tccgaactgc attgccttta gccgcagtgg caccgacgaa tatcccggct gcgtggtggt
    28261 catgtcgaac ggagatgatg gcgaaaaaac cattcatctg ggagagaatt acggcaataa
    28321 aacctggcgt gattttctgg ggaaccggca agagagcgta gtgacagacg aaaacggcga
    28381 agcgaccttc ttttgcaacg gcggcagcgt cagcgtgtgg gttatcgaag aggtgattta
    28441 aatccatccc cggcggcaag ccggggagat ttcattacgg cagtggcgtc ggtaacggcg
    28501 ctttatccag cgcggcggca cactctgccg tcgggcgatc cacacgctcc atcgtcatac
    28561 catcgtactc aatggtgtta ccgtcacgct caacctcgta cagctcacgt tttacggtaa
    28621 cgttagtgag atcatcagac atcaatgtca acttacccgg cacggcaatc ccccgctgcc
    28681 actgacggca atccaaggta tcgccctctt tagtgacaat caggctgcct atcgcttccg
    28741 gactgaccag cgcacgttgc ggccctttgg tctgccagta gcccgccagc cagtccggcg
    28801 cgggggtttt cacgacattg ttatagtttt cgacttcggc acagcccgcc agcaacatga
    28861 gcgcgcctgc aattgcgagt tttttcatca ttcgtcctgc atgagaagaa aagaggattg
    28921 tggcattaaa gctctgatgt cgccagcctt tccaggttca gagagaaatt tgatctaact
    28981 attatttttg tgaattatta ccccgaaaat aaatttgcat ggtgtatgat tcgcggctct
    29041 tgtttcaaac cttctgagtt cagaggctac attcatgtca tggcagcaat tcaaacacgc
    29101 ctggttgatt aaattctggg cacccatccc tgcggtcatc gcggcgggta ttctctccac
    29161 ttactatttt ggcattactg gcaccttttg ggctgtcacg ggtgaattta cccgttgggg
    29221 cggtcagctc ctgcagctgt ttggcgtcca tgctgaagag tggggttatt ttaaaattat
    29281 ccatctggaa ggatcgccat taacccgcat cgacggcatg atgatcctcg gtatgtttgg
    29341 cggctgcttt gctgctgcgc tgtgggccaa caatgtcaaa ctacgcatgc cacgcagccg
    29401 tatccgcatt atgcaggcca tcattggcgg cattatcgcc ggttttggcg cgcgtctggc
    29461 aatgggctgt aacctggcgg cgttctttac cggcattcct cagttctcgc tgcatgcctg
    29521 gttctttgcc atcgccactg ccattggttc atggtttggc gcgcgcttta ccctgctgcc
    29581 catcttccgt attcccgtga aaatgcagaa agtttctgcc gcctcaccgc tgacgcaaaa
    29641 accggatcag gcgcggcgtc gttttcgtct cgggatgctg gtctttttcg gcatgctggg
    29701 ctgggcgctg ctcacggcga tgaaccaacc aaaactgggg ctggcaatgc tgtttggcgt
    29761 cggctttggt ttactgattg aacgtgcgca aatctgcttt acttcggcgt tccgcgatat
    29821 gtggatcacc ggacgtaccc atatggcgaa agcaatcatt atcggtatgg cggtgagtgc
    29881 catcgggatc ttcagttacg tacagttagg cgttgaacct aaaatcatgt gggcgggacc
    29941 aaatgcggta attggtggtt tactttttgg ttttggcatc gtgctggcag gcggctgcga
    30001 aaccggctgg atgtaccgcg cggtagaagg ccaggtgcac tactggtggg tcggtctggg
    30061 caacgtgatc ggctcaacga ttctggcgta ttactgggat gatttcgctc cggcgctggc
    30121 caccgactgg gacaaaatca acctgctgaa aacttttggt ccgatgggag gcctgctggt
    30181 gacatatttg ctgctgtttg ctgcgctgat gttgattatc ggctgggaaa aacgcttctt
    30241 cccccgtgag gcaccgcaga ctgctaagga gatcgcatga aaaatatcgt tcctgattac
    30301 cgccttgata tggtgggtga accctgccct tatccggcag tcgcaaccct tgaggcgatg
    30361 ccgcaattaa aaaaaggtga gatcctggaa gtggtgagcg actgtccgca gtcgatcaat
    30421 aatattccac tggatgcgcg taatcacggc tacacggtgc tggatattca gcaagacggg
    30481 ccgaccattc gttatttaat tcagaagtaa tccctcattc ccgctgtggc ccacagcggg
    30541 aacatcgtta cctctgacac atagatttta cccgccccct ctcctattct tttcagtatg
    30601 atctcaggag gttgctatgt gtggacgctt tgcccaatcc caaacgcgtg aggattacct
    30661 tgcgcttctc gcggaagata ttgaacgcga tattccctac gatcccgaac ccattggcag
    30721 atacaatgtc gcgccgggaa ccaaagttct tctgctcagt gaacgtgatg aacaccttca
    30781 tctggatccg gttttctggg gatatgctcc cggatggtgg gataaaccgc cgctgattaa
    30841 cgcccgcgta gaaactgcct caaccagtcg catgtttaaa ccgctctggc aacatggtcg
    30901 ggcaatctgt tttgccgatg gctggtttga gtggaaaaaa gaaggcgaca aaaaaagcct
    30961 tattttatct atcgcgctga cggacaacct gtttttatgg ccgcgatagg tagcataccg
    31021 tttgagcgtg gtgacgaagc cgaaggattt ttgatcgtca ctgcggcggc agatcaaggt
    31081 ctggtagata ttcatgaccg acgcccgctg gttctgtcgc cagaagctgc acgggaatgg
    31141 atgcggcaag acattggtgg taaagaagcc tcagaaatcg ctaccagaag ctgtgtgcca
    31201 gcaaaccagt tcacctggca ccctgtgtcg cgcacggtgg gtaatgtcaa aaaccagggc
    31261 gcggagttaa ttcaacctgt ttgttgacat cctccacgcc ctgaatgacg aggacccctg
    31321 ctacgttcag gctgtcgcct gaatcatttc ggtgggttcc tgcttcaacg agcggcctga
    31381 ctgcaccatc tctccacagg caagaacggc gtgccccgcc gctaaaatgt tacgagctcc
    31441 atttacatcg gcgttcgctg tatatccaca tacctggcat ctgaatttac tttgtgacag
    31501 gcgattttct ttcgctgtat gaccacagca cgcgcaacgc tggcttgtgt acgctggcgg
    31561 aacagcaagc acctgaccgc cacgccagag ctgcttgtac tcaagctggc ggcgcatttc
    31621 ataccagccc tgatccagta tcgaacggtt taaacctgat tttgcccgga cattgcgccc
    31681 cggctggctg accgtacccg ctgctgactt tgacatgttg ctgaccttca aatcctcaat
    31741 gactatcatt acgtggtttt tgctgacggt cgttgtgact ttgtgaaggt agtccctgcg
    31801 gatatttgcg atacaggaat gcagtcgctg tattttgcgt ttctgcttct gccagttgtt
    31861 gctgaatttg accttgcggc ttaactgtcg ctgaagtctc gccagcttct tctggttttt
    31921 ctgaaaactg tttacaggct caaagactgt gccatctgac agcgaggcaa gtttagccac
    31981 gccagcatcc agcccgacca ttgatgctga agggtgaacc ggagttgata cttcactttc
    32041 tgcctgaata ctgatgtacc acttaccgca ggactggctg acagtgacat ttttcacaac
    32101 acccgtgacc tgacggctgt tccggtagcg catccagcca agttttggca gaaaaatacg
    32161 gctgttttcc tgagcgagct taacgccctg cggatagcgg aatgcatcat tctgtcaccg
    32221 ctttttgaat caggggaacg ccgagcgctt ctggaaaaag tttttgcagg cctgctcagg
    32281 gtctttcagt gactgttgca atggctgtga cggagcatct ttaatccatt gcgtttcagt
    32341 ggcatttttc cactcaacca atcaggaagc cattttcgcg taagggatgt ctttgttacc
    32401 ggccttatga ttctcattct gacgtgccag agtacgactg aaaacgaaac gacatgcgcc
    32461 tgcgaagcgc ctcatttcac gcgcctgttg accaccgggt cttaaccgga atttgaatac
    32521 ctgtagtcgt ttcacagggc aaattatact ttggtctgtg aaaaaaactg atatccgacg
    32581 cggaaggcac tgtgttttcc tgatgcatgc tcaccgggtc tttgttacca agtaccgacg
    32641 ccagattttt gaccatgacg cgacagaaaa actacgcact tacttttcaa atgtatgtgc
    32701 ttattttgaa gctgaactgg ttgaaatgga tggcgaacca gatcacgtcc atctgttaat
    32761 taactatccc cccaaactgg cgatatccag tctggtaaac aaacagcctc aaaggcgtat
    32821 cgggtaggtt actgcgacgg gatcgaccag atattgctgc caggtattac cgcaaagatg
    32881 ttttgtggcg tcccggctat tttgccagta gctgcggagg tgcgccaata tccgtcatcc
    32941 gccaatacat tgaacaacag caaacacctg gtcagatgaa aaaacgagcc ttatatcccc
    33001 gccctgaagg acgggctttt acggtgcact ggataagcct ggagattatc tacacctgca
    33061 tggacatcat tgtactatgc cccgtacatg ccccatcaca tcctcaacat caaagtatta
    33121 gagccttgca aaccattatg cgtctcagtt tgtctcatct atcttacgca aaaaaaatag
    33181 aactaatatc aagcggcgct atattgcgta ttaaataaaa ccacctcaat tacattaata
    33241 aatctaaata aatatcatgc aaataatttc catggttatt tttaggctat ttaaattaaa
    33301 aaaataaatt atgagtcaat cataaataac aacctttaat ataatttgac taccaaattt
    33361 atattcgttc acaaaatagt catgcattgt gtgcaataga aacagttcag ataaaaatag
    33421 ggatctactt cacaatttaa atgagaaact aaaacttaca tcttgaaata atctcattga
    33481 ttggatgaat atttgtagtg cagtgacatc atttttaaat aatagttcaa ataaaagggc
    33541 tcacgatgaa aaaattaaca gtggcaattt ctgctgtagc tgcatcagta ctgatggcga
    33601 tgtctgctca ggcagcagag atttacaata aagacagtaa caagctggat ctgtacggaa
    33661 aagttaatgc taagcactac ttctcttcta acgatgcaga tgatggcgac acaacttatg
    33721 ttcgtctggg cttcaaaggc gaaactcaaa tcaacgatca actgactggt ttcggtcagt
    33781 gggaatatga attcaaaggc aaccgtgctg aatctcaagg ttcttccaaa gataaaaccc
    33841 gtcttgcctt cgctggcctg aaattcggtg actacggctc catcgattat ggccgtaact
    33901 acggtgtagc atacgatatc ggtgcatgga ccgacgttct gccagaattc ggtggcgata
    33961 cctggaccca aacagatgtg ttcatgactg gtcgcaccac aggtgttgca acctatcgta
    34021 acaacgactt ctttggtctg gttgatggtc tgaactttgc tgctcagtat cagggtaaaa
    34081 atgaccgcac tgacgtaact gaagctaacg gtgatggttt cggtttctcc actacttatg
    34141 agtatgaagg attcggtgta ggtgcaacct atgctaaatc tgaccgcact aatgatcagg
    34201 ttatctacgg taacaacagc ctgaatgcat ctggtcaaaa tgctgaagta tgggcagctg
    34261 gtctgaaata tgatgcgaac aacatctatc tggctaccac ctattctgaa acccagaaca
    34321 tgactgtttt tggtaataac catattgcca acaaagcaca aaacttcgaa gtagttgcac
    34381 aatatcagtt cgacttcggt ctgcgtccgt ccgttgctta cctgcaatct aaaggaaaag
    34441 acttgggtgc gtggggtgat caggacctga ttgaatatat tgatgtaggt gcaacctatt
    34501 acttcaacaa aaatatgtcc acttttgttg attacaaaat caacctgatt gataagagcg
    34561 atttcacgaa agcatctggc gttgctaccg atgatatcgt tgctgtagga ttggtttacc
    34621 agttctaatc tgattactca aagatatgtt gtgggaggct ttgcctcccc aacatataag
    34681 tggcgctctc aagccacttt ccttagaagc actatcttgc ttctgactat ataaattacc
    34741 ctttattttc tgggacgttt caacgcccca tttttaataa tactcagtga acaattaacg
    34801 tatcaattag atttaataac aacgatatcc gtacctaacc tgatatctaa tgtcataagc
    34861 atccctttaa ttatcccctc aacctcttgt aaatttccca ctcttgagct ccacatagcc
    34921 caaacgccgg atgcaaaaaa atcacacgtg catctctgaa attgtgatgg aggaagtgag
    34981 tatcaattat atgtgcttat aaacgttccc gattggcaca gaagtcaccg gattgttcag
    35041 gctccaatag gttgagtatg tcgtatttat tttccaaaat aaaaatcgat gctgttgctc
    35101 cttaataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg ttatattaaa
    35161 acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt ccctggcaat
    35221 ctcgctgatg ggttttactg tcgtttgtat agcaggacaa cgcattattt aacctcacgt
    35281 cgtccaatat actttgaaaa cttattccct cgcttgccag acggcgatgc aatgtggaaa
    35341 cagaaatgta gagatatctc gctaccttgt ttgctgtcca ttttgttcta ggttcggata
    35401 gcattaggtt ataacaacga cttattaatg attgtttact atctgataaa agtaaacaat
    35461 taacatgatg tgctcctaat gaaagtagaa cgcccattgc taagtgttcc tgaatttgcg
    35521 ttgagaagcc tcgggaaata gatgttttta gttgctccca acaatatatt aactcggggt
    35581 tctgaggtaa gcagaaacct gtcgtgttac gaatttgatc agttatcgca taaaattttt
    35641 gaaacttttc aattagatca atgggataat aaagcatttc tgcaagataa agacctgctt
    35701 ctggataatt cacaatataa aattcatatc cacagggaaa taatattatc tgattattat
    35761 caacagttag actatgcgtc tcccaattga taactttttt tccctgacgg atacgacata
    35821 aagctggcat aagaggctta accctgtgaa tctcatgatg tttatgcatc cgtatttctt
    35881 caatctttag gttagactta tctcttgcca gcatactctt accctatttt atctcataaa
    35941 ctggtgttat ctcagcggtt gcaattttat tggcattaag catataacca actaacgctc
    36001 cgctggaatt agaatccaca ggaatcttat cagtttttag agcccatact ttaaactggt
    36061 aatgatgggg tttatctcct ttgggaggac atgctccacc aaatcctgca taaccaaaat
    36121 catttcggcc ttgaacagca ccagtcggaa gttttgtacc atcacgtctt cctgcatcag
    36181 tgggcaaata tgttacagtt gctggaatat tagcaacagt ccagtgccac catccactgc
    36241 ctgtaggagc atctggatca tacacagtta cggcaaagct tttggtgcct tcaggagcac
    36301 cagaccaggt taatgagggc gatgtattac caccttcaca cccaaatcca gaaaatacat
    36361 gagacgttgt aagttgctct cctgttttta tttcattact agtgacctga aatgctgcgg
    36421 cctgcgcaga aaatgttatg aatgccaata cacttgaaac gataagtttt ttcataaaaa
    36481 cctcattgtt gtgacctatc gttattttat ttgatgtgcc ttgatctcat tatgaataaa
    36541 ggcgcaatga tcgcgcaaat gcaatcataa ttataaaggt gccccgaaca ttagccataa
    36601 tatgcacaaa tcagattcct gttatctcaa ggagaattat tttttaatgc ggtgcacttc
    36661 gtgacaacaa gttaatgact aacacaccgg cacaaatcaa catcatgcct atgatagctg
    36721 gcaggtccag tcgttgacca aaaaatcccc aggaaagcaa actaatcaga acgataccga
    36781 ctcctgacca gatagcataa gcaatccctg taggtatata agccagcgtc tgagctaata
    36841 accagaatga tgcacaatag caaataattg tgccaacaga gggccataac cgtgtaaacc
    36901 cctctgaaaa cttcattaag gttgtaccaa tgacctctgc aagtattgcg ccaccaagat
    36961 aaatataagg gttcatagca tattctttcc tgttcatact ggagagaatt gtactacagt
    37021 ttgaacacaa ctcaccgttt tcatcctggt gtatgtgtat ccaaggggct ctggcgtggc
    37081 aagagataaa ggcaggaaca ataatgatca gaaggaaccc caggctaacg aatgagttca
    37141 ctcttcctgg gagcaaagcg aagacaaaac atgttatcgc gtttacggat cgtggatggc
    37201 agaaaacaac caggatcagg tatccatttt aaaccagaaa ttgagtgagt ttgccccatc
    37261 catgccccac gctgtgggat cggatataat taaacaagca tagtcaacag gttacacaac
    37321 ctacacctgc atggacatca cttcctgata cgccgccacc agcttattac gcacctgaat
    37381 ccccatttgc atagaaactg aggctttttg catatcggtc atcacatcgt ttagcgccac
    37441 gccgggttca ccgagggtga atttttctgc ctgcgtgcgg gcgactgttt gtgtatcgct
    37501 tatgcgatcg agcgcggcgt gcagctgccc ggcaaaacta atggtcggtt gcggcagtga
    37561 ttcctgcgca cacgcactca tcgccgtagc ctgtaactgg ctgataaccc cttcaatccc
    37621 ctgtatcgct gacattttcg tctcccggat aatttctggt aacaaagcct accagtaagt
    37681 caataagaca aaggcgctaa atagcaacaa aaaaactggt ttattggcgg atagaaaaaa
    37741 cgaaagcaca aataatggga gcgtcaattt ttcgagtttg ctgacccgag agtgagtctt
    37801 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
    37861 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
    37921 ttgattgtta ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
    37981 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
    38041 agccaactga cgcaaatgaa tattccttac cgcttcagcg aagccagcgg cgctattgag
    38101 gttccggcag ataaagttca cgaactgcgc ctgcgcctgg cgcaacaagg tttgcccaaa
    38161 ggcggcgcgg tcggtttcga actgctcgat caggaaaagt ttggtatcag ccagttcagc
    38221 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
    38281 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
    38341 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
    38401 gaagggcaaa ttagcgctat tgtacatctg gtttccagcg cggttgctgg tctgccgcct
    38461 ggaaacgtca cgctggtgga tcagggcggg catctgttaa cccagtccaa taccagcggg
    38521 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcag
    38581 cgtattgaag cgatcctgtc acctattgtt ggtaacggta atattcacgc ctaggtcacg
    38641 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
    38701 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
    38761 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
    38821 atcagcacgc ctccgacaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
    38881 agtgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
    38941 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
    39001 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
    39061 gaagctctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgattc gctcaatgtt
    39121 gttaactcgc cgttcaatag cagtgacgaa agcggcggag cactgccgtt ttggcaacag
    39181 caagtgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggttgc tggtactgct
    39241 ggtggcgtgg ttgctgtggc ggaaagcggt acgcccgcag ctaacacgtc gcgccgaggc
    39301 ggtgaagact gtgcagcaac aggcgcaggc ccacgaggaa gtggaagatg cggtggaagt
    39361 ccgtctgagc aaagacgaac aactccaaca acggcgcgct aaccaacgac tgggggcaga
    39421 agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat ccgcgcgtgg tggcgctggt
    39481 cattcgccag tggataaata acgatcatga gtaacctgac aggcaccgat aaaagcgtca
    39541 tcctgctgat gaccattggc gaagaccggg cggcagaggt gttcaagcac ctctcccagc
    39601 gcgaagtgca aaccctgagc gctgcaatgg cgaacgtcac gcagatctcc aacaagcagc
    39661 taaccgatgt gctggcggag tttgagcaag aagctgaaca gtttgctgca ctgaatatca
    39721 acgccaacga ttatctgcgt tcggtattgg tcaaagctct gggtgaagaa cgtgccgcca
    39781 gcctgctgga agatattctc gaaacccgcg ataccgccag cggtattgaa acgctcaact
    39841 ttatggagcc gcagagcgcc gccgatctga ttcgcgatga gcatccgcaa attatcgcca
    39901 ccattctggt gcatctgaag cgcgcccagg ccgccgatat tctggcgttg ttcgatgaac
    39961 gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg cggcgtgcag ccagccgcgc
    40021 tggccgaact gaccgaagta ctgaatggct tgctcgacgg tcagaatctc aagcgcagca
    40081 aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct gatgaaaacg cagcaggaag
    40141 aagccgttat taccgccgtg cgtgaattcg acggcgagct ggcgcagaaa atcatcgacg
    40201 agatgttcct gttcgagaat ctggtggatg tcgatgatcg cagcattcag cgtctgttgc
    40261 aggaagtgga ttccgaatcg ctgctgatcg cgctgaaagg ggccgagcag ccactgcgcg
    40321 agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat tctgcgcgac gatctcgcca
    40381 accgtggtcc ggtgcgtctg tcgcaggtcg aaaacgaaca gaaagcgatt ctgctgattg
    40441 tgcgccgtct tgccgaaact ggcgagatgg tgattggcag cggcgaggat acctatgtct
    40501 gataatctgc cgtggaaaac ctggacgccg gacgatctcg cgccaccacc ggcagagttt
    40561 gtgccaatgg tcgagtcgga agaaaccatt attgaagagg ccgaacccag ccttgagcag
    40621 caactggcgc aactgcaaat gcaggcccac gagcagggtt atcaggcggg gattgccgaa
    40681 ggtcgccagc aaggtcacga acagggctat caggaaggac tggcccaggg gctggaacaa
    40741 ggtctggcag aggcgaaggc tcaacaagcg ccaattcatg cccggatgca gcaactggtc
    40801 agcgaatttc aaactaccct tgatgcgctt gatagtgtga tagcttcgcg cctgatgcag
    40861 atggcgctgg aggcagcacg tcaggtgatc ggtcagacac caacggtgga taactcggca
    40921 ctgatcaaac agatccaaca gttgttgcag caagaaccgt tattcagcgg gaaaccacag
    40981 ctgcgcgtac acccggatga tctgcaacgt gtggatgata tgctcggcgc aactttaagt
    41041 ttgcatggct ggcgcttgcg gggcgatccc accctccacc ctggcggatg taaagtctcc
    41101 gccgatgaag gcgatctcga cgccagtgtc gccactcgct ggcaagaact ctgccgtctg
    41161 gcagcaccag gagtggtgta atgaccacgc gcctgactcg ctggctaacc acgctggata
    41221 actttgaagc caaaatggcg cagttgcctg cggtacgtcg ctacgggcga ttaacccgcg
    41281 ctaccgggct ggtgctggaa gccaccggat tacaattgcc gctcggcgca acctgtgtca
    41341 ttgagcgcca gaacggcagc gaaacgcacg aagtagaaag cgaagtcgtt ggctttaacg
    41401 gtcaacggct gtttttaatg ccgctggagg aagtggaagg tgtcctgccc ggcgcgcgtg
    41461 tatatgccaa aaacatttcg gcagaagggc tgcaaagcgg caagcagttg ccgctcggtc
    41521 cggcgttatt aggtcgtgtt ctggacggta gcggtaaacc gctcgatggc ctgcccttcc
    41581 ccgatacgac ggaaaccggt gcgctgatta ccccgccatt taacccgttg caacgtacac
    41641 cgattgaaca tgtgctggac accggcgtgc gcccaatcaa tgccctgctt accgtcggac
    41701 gcgggcagcg tatggggctg tttgccgggt cgggcgttgg taaaagtgtg ctgctgggga
    41761 tgatggcacg ctacacccgc gccgatgtca ttgtcgtggg tttgattggt gaacgtgggc
    41821 gcgaagtaaa agattttatt gagaatatcc tcggtgccga aggacgtgca cgctcagtgg
    41881 tgattgccgc tccggcggat gtttctccgc tcctgcgaat gcaaggtgcc gcctatgcca
    41941 cgcgcattgc cgaagatttt cgcgatcgtg gtcagcatgt gttgctgatt atggactccc
    42001 tcacccgcta cgcgatggcc cagcgtgaaa ttgcgctggc gattggcgaa ccacctgcca
    42061 ctaaaggtta tccaccgtcg gtgtttgcca aattaccggc actggtcgag cgtgccggta
    42121 atggcattag cggcggcggc tcgattaccg cgttttatac cgtgctcacc gaaggcgatg
    42181 accagcagga ccccattgct gactccgcgc gggcaatcct cgacggtcac attgtgctgt
    42241 ctcgccgact ggcggaagcc gggcactatc cggctatcga tattgaagcg tcgatcagtc
    42301 gcgcaatgac ggcgttgatc agtgagcaac attacgcgcg agtgcgcacc ttcaaacagc
    42361 tgttgtcgag ttttcagcgt aaccgcgatc tggttagcgt cggcgcgtat gccaaaggca
    42421 gcgatccgat gctcgataaa gccatcgccc tgtggccgca gctggagggc tatttgcaac
    42481 aaggcatttt tgaacgcgcg gactgggaag cgtctctcca ggggctggag cgtattttcc
    42541 cgacagtgtc ataacccagg agataacggc agatggcgga agaacagctc aaaatgttga
    42601 tcgattatca gaatgaatat cgcaataacc tcaatagcga tatgagtgcc gggatgacca
    42661 gcaaccgctg gatcaactat cagcagttta tccagacgct ggaaaaagcc attactcagc
    42721 atcgccagca acttaatcag tggacgcaga aagttgacat tgccctgaac agctggcgag
    42781 aaaagaaaca acgtttgcag gcctggcaga cactgcagga acggcaatcc acggcggcac
    42841 tgcttgcaga aaaccgcctc gatcagaaaa agatggatga gttcgcccag cgcgccgcca
    42901 tgaggaaacc tgaatgattc gcttagcgcc cttgattacc gccgacgttg acaccaccac
    42961 attgcctggc ggcaaagcca gcgatgctgc acaagatttt ctcacgttgt tgagcgaagc
    43021 attagcaggc gagacaacaa ctgacaaagc ggccccccag ttgctggtag caacagataa
    43081 gcccacgaca aaaggcgagc cgctggtcag cgatattctt gccgatgcgc aacaagcgga
    43141 tttactgatc cctgtggatg aaacacttcc tgtcatcaac gacgaacaat ccacatcaac
    43201 accgttaacc accgctcaga cgatgacgtt ggctgcggtg gctgacaaaa atacgacaaa
    43261 agacgaaaaa gcggatgatc ttaatgaaga cgtcaccgca agtctgagcg ccctttttgc
    43321 gatgttgccg ggttttgaca atacgcccaa agtgactgat gcaccgtcaa ccgtgttacc
    43381 ggcagagaaa ccaacgctct tcacaaaact gacttctgcg caactcacta cagcacagcc
    43441 tgatgacgcc cccggcacac cagctcagcc attaacaccg ctggtagcag aagcccagag
    43501 taaagcggaa gtcatcagca cgccttcgcc ggtgaccgct gacgccagcc cgctaatcac
    43561 tccacaccag acacagccac tgcccaccgt cgccgcgcct gttttgagtg caccgctggg
    43621 ttctcacgaa tggcaacaat cattgagcca gcatatttcg ctgttcaccc gccaggggca
    43681 acaaagtgca gagttgcgtc tgcacccaca ggatttaggt gaagtgcaaa tttccctcaa
    43741 agtggatgat aaccaggcgc agatccagat gatttcaccg catcagcacg tacgcgccgc
    43801 cctggaagca gcgctgccgg tactgcgtac gcagctggcc gaaagtggca ttcagttagg
    43861 gcaaagcaac attagtggcg aaagctttag tggtcagcag caggccgctt cccagcaaca
    43921 gcaaagccaa cgcacagcaa accatgaacc tctggcgggg gaagacgacg atacgcttcc
    43981 ggttcccgtc tctttacaag ggcgtgtaac aggtaacagc ggcgttgata ttttcgccta
    44041 acgtcagagg tagcaccgta atccgcgtct tttccccgct ttgttgcgct caagacgcag
    44101 gataattagc cgataagcag tagcgacaca ggaagaccgc aacacatgac tgattacgcg
    44161 ataagcaaga aaagcaagcg atcgctttgg atcccgattc tggtattcat taccctcgcg
    44221 gcctgtgcca gcgcaggtta cagctactgg cattcgcatc aggttgccgc tgacgacaaa
    44281 gcgcagcaac gcgtcgtgcc ctccccggtc ttctatgcgc tggatacctt tacggtcaat
    44341 ttgggcgatg cggatcgcgt gctttatatc ggcataaccc tgcgactgaa agatgaagca
    44401 acccgctcgc ggctgagtga gtatttgccg gaagtccgta gtcgcttgct gttactgttt
    44461 tcacgtcagg atgctgccgt actggcgaca gaagaaggca agaaaaacct gattgccgag
    44521 attaaaacca cactttccac cccgcttgtt gccgggcaac cgaaacagga tgtcaccgac
    44581 gtgctgtata ccgcttttat tctgcgataa cgacatgggc gatagtattc tttctcaagc
    44641 tgaaattgat gcgctgttga atggtgacag cgaagtcaaa gacgaaccga cagccagtgt
    44701 tagcggcgaa agtgacattc gtccgtacga tccgaatacc caacgacggg ttgtgcgcga
    44761 acgtttgcag gcgctggaaa tcattaatga gcgctttgcc cgccattttc gtatggggct
    44821 gttcaacctg ctgcgccgta gcccggacat taccgtcggg gccatccgca ttcagccgta
    44881 ccatgaattt gcccgcaacc tgccggtgcc gaccaacctg aacctaatcc acctgaaacc
    44941 gctacgcggc acagggctgg tagtgttctc gccgagtctg gtgtttatcg ccgtggataa
    45001 cctgtttggc ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt ttacccatac
    45061 tgaacagcgc gtcatcaacc gtatgttgaa gctggcgctg gaaggctata gcgacgcctg
    45121 gaaagcgatt aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg tggaatttac
    45181 caatatcacc acctcgccga acgacattgt ggttaacacg ccgttccatg tggagattgg
    45241 caacctgacc ggcgaattta atatctgcct gccattcagc atgatcgagc cgctacggga
    45301 attgttggtt aacccgccgc tggaaaactc gcgtaatgaa gatcagaact ggcgcgataa
    45361 cctggtgcgc caggtgcagc attcacagct ggaactggtg gccaactttg ccgatatctc
    45421 gctacgcctg tcgcagattt taaaactgaa acccggcgac gtcctgccga tagaaaaacc
    45481 cgatcgcatc atcgcccatg ttgacggcgt cccggttctg accagtcagt acggtactct
    45541 caacggtcag tatgcgttac ggatagaaca tttgattaac ccgattttaa attctctgaa
    45601 cgaggaacag cccaaatgag tgacatgaat aatccggccg atgacaacaa cggcgcaatg
    45661 gacgatctgt gggctgaagc gttgagcgag caaaaatcaa ccagcgaaaa aagcgctgcc
    45721 gacgcggtgt tccagcaatt tggcggtggt gatgtcagcg gaacgttgca ggatatcgac
    45781 ctgattatgg atattccggt caagctgacc gtcgagctgg gccgtacgcg gatgaccatc
    45841 aaagagctgt tgcgtctgac gcaagggtca gtcgtggcgc tggacggtct ggcgggcgaa
    45901 ccgctggata ttctgatcaa cggttattta atcgcccagg gcgaagtggt ggttgttgcc
    45961 gataaatatg gcgtgcggat caccgatatc attaccccgt ccgagcggat gcgccgcctg
    46021 agccgttagt gatgaataac catgctactg tgcaatcttc cgcgccggtt tctgctgagc
    46081 cactgctgca ggtgagcggc gcactcatcg ccattattgc cctgatcctc gctgctgcct
    46141 ggctggtaaa acggctggga tttgccccta aacgcactgg cgttaacggt ctgaaaatta
    46201 gcgccagtgc ttcgctgggc gcgcgtgaac gggtcgtggt ggtcgatgtg gaagatgcac
    46261 ggctggtgct cggcgttacc gcagggcaaa tcaatctgct gcataaactt cccccttcag
    46321 caccaacgga agagataccg cagaccgatt ttcagtcggt catgaaaaat ttgcttaagc
    46381 gtggcgggag atcctgatgc gtcgtttgtt ttctgtcgca ccggtccttc tctggctgat
    46441 tacgcccctc gccttcgcgc aactgccggg tatcaccagc cagccgctac ctggcggtgg
    46501 acaaagctgg tcgctcccgg tgcagacgct ggtgttcatc acctcgttga cgttcattcc
    46561 agcgatttta ctgatgatga ccagtttcac ccgcatcatt attgtttttg gtttattgcg
    46621 taacgcgctg ggaacgccct ccgcgccacc taaccaggta ttgctggggc tggcactgtt
    46681 tttgaccttt tttattatgt cgccagtgat caacaaaatt tatgtcgatg cgtaccagcc
    46741 attcagcgaa gagaaaatct caatgcagga ggcgctggaa aaaggggcgc agccgctgcg
    46801 tgagtttatg ctgcgtcaga cccgcgaggc agatttaggg ttgtttgcca gactggcgaa
    46861 taccggcccg ttgcaggggc ctgaagctgt gccgatgcgc attttgctcc cggcctacgt
    46921 gaccagcgag ttgaaaaccg catttcagat aggcttcacg attttcatcc cttttttgat
    46981 tatcgacctg gtgattgcca gcgtgttgat ggcattgggg atgatgatgg ttccccccgc
    47041 caccattgct ctgcccttta aactgatgct gtttgtactg gtggatggct ggcaattgct
    47101 ggtcggttcg ctggcgcaga gcttttacag ctagagaggc aaaatgacac ctgaatcggt
    47161 catgatgatg ggaactgaag cgatgaaagt cgcgctggca ctggcagccc cgctgttgct
    47221 ggtagcgctg gtcacgggcc ttatcatcag tattttgcag gcagccacgc agattaacga
    47281 aatgacgctg tcgtttattc cgaaaatcat cgccgtattt atcgccatca ttattgccgg
    47341 accgtggatg ctcaatctgt tgctggatta cgtccgcacc ttgttcacta acctgccgta
    47401 tatcatcggg tagcccgtac aatgatgcag gagacaagcg atcaatggct ttcctggtta
    47461 agcctgtact tctggccgtt actgcgcgtg ctggcgctca tttctaccgc gccaattctg
    47521 agcgaacgca gcgtgccgaa acgggtaaaa ctgggtctgg caatgatgat cacgttcgcc
    47581 attgccccat cattacctgc caacgatgtt cctgtttttt cgttctttgc tctgtggctg
    47641 gccgtacagc agatcctgat cggcattgcg cttggtttta ccatgcaatt tgcctttgcc
    47701 gctgtgcgaa ccgctggcga aattatcggt ctgcaaatgg ggctgtcatt tgcgacattt
    47761 gtcgatcctg gcagccatct caatatgccc gttttagcgc gcatcatgga tatgctggca
    47821 ttactgctgt tcctgacatt taacggtcat ttatggttga tttcactgct ggtcgatacc
    47881 tttcacaccc tgccgattgg tagcgaaccg ttgaacagca atgcgtttct ggcacccacc
    47941 aaagcaggga gtttgatttt ccttaacggg ctgatgctgg cgttaccgct cattactctg
    48001 ttgctgaccc tgaatctggc attaggttta ctgaatcgta tggccccgca attatccatt
    48061 tttgttattg gatttccatt aactctgact gtcggcatct ctttaatggc ggcattaatg
    48121 ccgttaattg cgcctttttg cgaacattta ttcagtgaaa tttttaattt gctggctgat
    48181 attattagtg aattaccatt aatataattc cgtaacgttt atcatgctac cctaaggatt
    48241 atccgaaaaa taatatctgc gaacatcttc caggatactc ctgtagcgaa atatttgttt
    48301 taagctcact cacatatcgc aacatttact ttaagacaat tccaggcaaa ttatacaaca
    48361 ctttacggga tagtaagtcc gcctgaaaaa ttgcgagagt ggcgcattag gtgacccatg
    48421 ttgttccgtt tagtcatgat gaaatattca ggtaagggga attatcgtta cgcattgagt
    48481 gagggtatgc catgtcaacg attattatgg atttatgtag ttacacccga ctaggtttaa
    48541 ccgggtatct gttgagtaga ggggttaaaa aaagagaaat caacgacatt gaaaccgttg
    48601 atgaccttgc catagcttgt gattcacagc gtccttcagt ggtgtttatt aatgaggact
    48661 gtttcatcca cgatgcttct aacagtcagc gcatcaagca catcattaat caacatccca
    48721 atacgttatt tatcgttttt atggcaattg ccaatgttca ttttgatgaa tatctattgg
    48781 tcagaaaaaa tttattgatc agttctaaat cgattaaacc ggaatctctc gacgatatcc
    48841 ttggcaatat tctgaaaaaa gagacaacga taacctcgtt tttaaatatg ccgacgttat
    48901 cattgagccg aaccgaatcg agtatgttgc gaatgtggat ggcaggtcag ggaaccattc
    48961 aaatctccga ccaaatgaat atcaaagcca agaccgtttc atcgcataaa ggtaatatta
    49021 aacgtaagat caaaacgcat aataaacagg ttatctacca tgtcgtccga ctgacggata
    49081 atgtgactaa tggtattttt gtcaacatgc gctaacacat tctgactggt gatttcccac
    49141 cagtcaggct gaataagatt actctgcttt ctcaacaaag ataccgtcct gatgccctgc
    49201 ttcattaaag aaccagatgc cgagcgggta gtcttccagc gcaaccaggt acattgtgcc
    49261 ttcactaaac tcctcaactg ccagtaccac tccaggacga cgcggaccgc catccgtttt
    49321 gactgttacc cgatcattca ccttcatcgt tttcctcctg tggctttgtg ccagtgtaga
    49381 acaatttcgt ttttctggca gcgccaagcg cgcgcgagtg ctgattttct cgacggtcta
    49441 tacttaagag atgctaggcg gacttaacga ctggcggcaa caacagagta acggttgcga
    49501 ggaaagatga tgaaaaccgc aaaagtgtac agcgataccg caaaacgtga ggtcagcgtc
    49561 gatgtcgatg ccctgctggc ggcgatcaat gaaattagcg aaagcgaagt tcatcgcagc
    49621 cagaacgatt ctgaacacgt tagcgtcgat ggacgtgaat atcatacatg gcgtgaattg
    49681 gcggatgcct tcgaactgga tattcatgac ttcagcgtct ctgaagtgaa tcgttgaatt
    49741 cacaataaaa aaatcccgac cctgagggtg tcgggatgaa acttgcttaa gcaagaagca
    49801 cttaaaaaat tcgttacacc aggaaatctg atgtgttcat caccttatct gcaatttttt
    49861 tcgctgacaa gaaaatattc gcgctatgaa tgattaacgc tacttatgaa tagaaatgtg
    49921 acgcgcgtca ccccttggtg gctctggtgt caacgggatc gctcccgaaa aaatcatctt
    49981 tgattatttg tgctgttttg agaatcatcc tggtaagggg agtactgcgc gaccatgttt
    50041 tcaattcaac aaccactact ggtttttagc gatcttgatg gcaccctgct ggacagtcat
    50101 agttatgact ggcaaccggc agccccctgg ctcagccgtt tacacgaagc caatattccc
    50161 gtcattctct gtagcagtaa aactttagcg gaaatgctgt acttgcaaaa aatgttgggg
    50221 ctacaaggct taccgctgat tgcagagaat ggcgcagtga tccagcttgc tgaacaatgg
    50281 caggatatag acggttttcc acgcatcatc tcaggtatta gccatggcga aatctgccag
    50341 gttttaaata cgctacgcga gaaagaacat tttaaattca cgacttttga tgatgtcgac
    50401 gatgcaacca ttgccgaatg gacgggatta agccgtagct aggcggcgct gacgcagctg
    50461 catgaggcgt cggtaacgct aatctggcgc gacagtgacg agcgtatggc acaatttatc
    50521 gctcgtctga acgaactggg cttgcagttt atgcagggtg cgcgcttctg gcacgtactg
    50581 gatgcctctg ccggaaaaga tcaggctgcc aattggatta tcgcgaccta tcaacaattg
    50641 tcaggcagac gcccaaccac acttggcctg ggcgatgggc caaacgatgc gcctttactg
    50701 gaggtaatgg attacgcggt gattgtgaaa gggctaaacc gtgaaggggt acatctgcat
    50761 gatgaggatc cggcccgcgt ctggcgaacg cagcgtgaag gaccggaagg ctggcgtgaa
    50821 gggctggacc attttttctc cgcccgttaa gcgttatcgc tcgcgaatac ccgattgcgc
    50881 ccggcctgtt ttgcgaggta aagccgacgg tcggccaatg actggagttg ttcaaaatca
    50941 taatcaccgg tttcctcgct gctacttacc cccagcgagg cactgatgcg tatcgttgta
    51001 ctcttggcga tcagcatctc tttttcattt aacttcaggc gaatacgttc tgcgacctcc
    51061 gcagcctgcg tcagattcgc gcctggcaga atcacacaaa actcctcacc accgacccgc
    51121 ccggcaacgt cctgcgcacg caaggaactg ctaattaatc cggcagcatg agaaagaaca
    51181 cggtcgcccg cctgatgacc aaagcggtca ttaatcgctt taaaatggtc aagatcgacc
    51241 tgaatgacag aaaaaggatg ttggtgcgtc tgacacaatt tagcgagcgg acgggctttt
    51301 tcgaacagtg cgccacggtt atataaacgc gttaaggtgt cgtgccacgc ctgccactgc
    51361 aacgagcttt gcagaacata catgttgctg accatccggc gaatcacata ccaggagagg
    51421 agtaacatgg tggtaaagag cgcccacagc agggttaatg caatgctgat actgccgaaa
    51481 tcgccgcgca cgccttcgct tagcgtatgg acgcgcgcca gcacaccgtc gaaatggtcc
    51541 agacgttccc agctaacata gcgactgtcc atacgaatgc cgccccgcgt gtcatgttcc
    51601 attgcctgcg ccagcaaggc cagttcacga ggatcaaaaa tattccctgt tggatggtca
    51661 ggattggaag aggtcaaaaa tctcagctta ctgtcataga gctgatactc accatcgagg
    51721 tttttatcga tggcgtttct taaaaattgc tgcatagtac gcacgggaat actcatcccc
    51781 agcacgccat accagtagtt attactgtct accggaacac tgacggtaac ctgcggttca
    51841 gtattgctgg cgtgttccgg ttgcgaagtg aaccagcgta ccgcgcggtg acgattttct
    51901 cgttgcgaat ggccgataaa ccagggttgg gtgacatagc cgtaataacg cgttggtaca
    51961 ttgcgcgtaa acaaggtcgg ctgcgtcgaa acgtaaaatc cagcacgcga gacatacatc
    52021 gcctgttcaa ccatcgacga ggagttgtgc gccagtcgca gcaagtaacc aacttccagt
    52081 gcagcggtaa tttcattgtc gaggctttca ttttcgcgag acaggaaatt cccctcgctg
    52141 actaatgcat ccgatacgcc attgactggc agggtgcgcc gtcggttgag ttcgatttgc
    52201 caggcgtgct cgtcgcgatg ctgttcgaac tcggttacgg cattacgcag agaggtgaaa
    52261 tccagtggcg ccacgagagc ttcgcgcatg ccattacgca aaaagatcag tttgtcgacg
    52321 ttatactgca aatgcttatc gagcgcgttg gctacgtttt ccagatgatt acgctagctg
    52381 gagatatagg catcttccag caccaccact tcacgccagg tgagcaaggt ggaaaaaagc
    52441 aataccacga taaagcagag attaacgaca tgaccgggcc ccaggcggcg tgcgagtttt
    52501 ttcaaccagc tctggttttc catttttgtc tcgtgctgca ccctgacgac tcccaacctg
    52561 cttttttatg attctggcat aacccggcaa tagcgtaccg caaaacagcg ataattttat
    52621 aatggtggca tgataagacg cgactatcat tcattgcagg cattgggaca ccaggccgga
    52681 tgtgatccgg cctggtgtta atgataccct ttcaaacttc ttccgcagaa cgactcccct
    52741 cccccggaac aagatcggtt tcatgaaaag cttcccgctt gaccccgtaa ccgtcatacc
    52801 aacgacactc aaccataccg ctggagtatc cagtgacaat catccgcggg ccgccctctt
    52861 taaccgtaac ttcctcacta accataaagc tcatactcgc ctcctttttt cgagtgaaac
    52921 ttgttcacct tagttgaaga tggcgaattt tgcataatag ccagtgcgag tattaatgtg
    52981 cctgaccgcg cattttcgct actgctttca ctcccagcac cacgatgccg ccgatgataa
    53041 atccaagaat cagatttaaa acagtcggta atatcatcgc caccactgca ctttgctgcc
    53101 cggcgatatg ttcaatagcg tgatgcagcg gcgcaatacc atgtaccaca atcccgccgc
    53161 cgacgaggaa catcgccagc gtgccgacaa tcgataatgc tttcatcagc caaggcgcga
    53221 taatcaataa ccctttacct aatgcctgca tcagcgcgct agatttttcc gccagccaat
    53281 accctaggtc atcaatctta acgataaccc ctaccagacc gtagacgccc acggtcacta
    53341 ccagcgcgat gcctgaaagc accagcacct gattaagcaa cggcgcctct gccacaatcc
    53401 ccagcgtgat ggcgacgatt tccgcagaca aaataaaatc ggtacgaatc gcccctttaa
    53461 ttttgtcctt ttcaaacttc agcggatcct gcgccgccag cttctccaga cgctgctggc
    53521 tctgcgctgg atcttcttta tgtttacgcg cctccagcat atgcagcact ttctctaccc
    53581 cttcaaagca gagaaacgcg ccaccaatca tcaacagtgg cgtaatcgcc cacgggataa
    53641 acgcactgat gatcagcgcc agcggcacca gaatcacttt attgatcagc gatcctttcg
    53701 ccacgcccca gaccacggga agttcccggt tggcccgcac gcctgaaact tgttgcgcat
    53761 tgagcgataa atcatcccct aatacaccgg cggttttctt cgccgccagt ttgcccatca
    53821 cagagatatc gtccagcagt gtggcgatat catcgagcaa cgtcagtaaa ctactcccgg
    53881 ccaaaagcat tccctctcat ttttattgtt gaagggtgta agtatgaagc aaaaacggca
    53941 gctctgatac ttgcttcaca ggtcaacatt tatttaacaa tcaagtgcaa ttatatctct
    54001 cgccagcaga atgattttca cgtttactat gaaccacctt tttatttccg ccgtgaggga
    54061 ttatgcgttt ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt
    54121 ggggctcaac ctattttgtc attcggattg gcgtgggaag ctggcctccg ttaatgatgg
    54181 cgggcgttcg attccaggca gccggtattt tattgctggc atttttgcta ctgcgcggac
    54241 acaaacttcc cccgctacgt ccgctgctca atgccgcgct gattggcctg ttattgctgg
    54301 ctgtcggtaa tggcatggtg acggttgccg aacatcaaaa tgttccttcc ggcatcgccg
    54361 ccgtagtgat tgcaaccgtg cccctcttta ccctgtgctt cagtcgcctg tttggcatta
    54421 aaacgcgcaa actggaatgg gtgggtattg ccattgggct tgccggaatc atcatgctca
    54481 atagcggtgg aaatttaagc ggcaatccgt ggggcgcgat tctgatttta atcggctcga
    54541 ttagctgggc gtttggctca gtttatggct cgcgcattac cttacctgta gggatgatgg
    54601 cgggtgcgat tgagatgctg gcggcaggcg tggtgttaat gatcgcatcg atgattgcgg
    54661 gtgaaaaact gacggcgctc ccttccctgt caggcttcct tgcggtcggc tatctggcgc
    54721 tgtttggttc gattatcgcc attaacgctt atatgtattt aatccgtaat gtcagtccgg
    54781 ctctcgccac cagctacgcc tacgttaacc cggtggtcgc ggtcttgctg ggtacgggac
    54841 tgggtggcga aacgctgtcg aagattgaat ggctggcgct cggcgtaatt gttttcgcgg
    54901 tggtactggt cacgttggga aaatatctct tcccggccaa acccgttgtc gcgccagtta
    54961 ttcaggatgc atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct
    55021 tcgccgcaga tccactcttc cagacgctcg gtaagcgccg catccgtcag cttctcgcgc
    55081 ctacgtaacg cacactccca gacaatcagt acgcgccagc cgagctcctg caagcgactg
    55141 atatcgcggc gatcgcgttc aacattttta cctatcttct ccagccagaa ctcagttcga
    55201 gtcgcaggca ctttaaacag atagcagtga tgatgatgcc agaagcagcc gtgggtaaat
    55261 atcacgcagc gatattcatc aacgacaaaa tccgggcgtc cgggcagact ggcgtcctga
    55321 acgcgaaacg caaggccctg cccggttaac aaactggcga ggcgcttctc aatcgccgta
    55381 tcacgcgtgg caatcgcgcg catatttttg ctgcgggtgg ccttatcgtg aacgtcgacc
    55441 atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg atttttggct caagcagttt
    55501 tgccaccgcg gcaaagaccg gcacgaccac cgagttgccg aactggcgat aggcctgagt
    55561 gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc
    55621 gcattcacga ggcgttaacc gacgtggacg atgttgctga ttcagcggat cgtcaaagtc
    55681 tttctcaccc gtggccatat cccaaccgcg atcgattaaa atttccgcgc catctttgta
    55741 ataacgagca gacagcgtac gggtgacgct ttgcggattg ttcggataaa ccattccata
    55801 accgaagccg ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt acttccacag
    55861 caccggcgtc aggatatatt tcgcctcaac catcgggtcc agcagctgcg ccagcgtcac
    55921 tcgctgtgca gggaaacatt cgctgatatc acgcagagta aaatcggctt tcagattcag
    55981 atcgcgacga aaacccacca gcacgatgcg ttcacggtgc tgcggcagga aatgtttacc
    56041 atcgatgatt ttcggatcgt ccggcccgtt atcttctgca tcggcaacgt catagcccag
    56101 ttcgtccagc gtctgcatga tgatgcggaa cgtttttccc ttatcgtgac tcttcaggtt
    56161 tttgacgttt tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc gcaccacatc
    56221 aaagaacagc gtgccctggg tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt
    56281 tttcgatacg ccagccagcg aaaatggctg acaagggaaa ccggccagta aaacatcgtg
    56341 ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca tcactcacgc cttcttgatg
    56401 actgagggtg atgtcgcgga tatcttcatt aaaatgatgc gtcgccgggt cgcaataatg
    56461 gttggcttta taagtgcgta ccgcatgttt gttccattcg ctggtaaaca cgcactgtcc
    56521 accaatcgat tcaaaaccac gacggatgcc gccaattccg gcaaatagat cgataaagcg
    56581 aaacgcataa tgcggatgat gttccggtgg tttgggtaac aacgtttgca gatgggcaaa
    56641 ctctttctca cttaaacggt gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc
    56701 cgcgctccag tgattctcac ccacaccatt aagctgcgcc accagcattt taacatcata
    56761 aatttgcagc agtttctcca gcattgcctg tgcggcattc ccggtgctgt atgaatcggt
    56821 tactgatata ttttcctgca tagatttaac cggccatctg agatggccga caggttaaca
    56881 taattactgc cgtttagggc cacgcgctca gccagcgggt gaaaacgcat ctatcacctt
    56941 gtgatcgata ccttcattct cccccgccag ttcggcaccg agcttcgcca tcaaacagcg
    57001 ccaagctcaa tgcccctgaa acggcaaata cgcgcgccag gccaatcgcc cccagggctt
    57061 ccagccgatc cgcatcctgg acgattttag cttccgttgt taagggggcg atttgcgcgc
    57121 tgaaactgtg agcggcaatg gcatgacaaa cggcctcaat tttctccgcc ggaaattgca
    57181 cgaactcttc acggagcagg cgacgcgtct cttctgccgc caggattgaa gaacgctgcc
    57241 gttgcggatg atttttcgcc aggctcacga tatcgtgaaa ataacaggcg gttaaaatca
    57301 ccagcatatc gacatcgtca tctgccgcca gtttctgtgc cgtagcccag acacggcgaa
    57361 aatgacacag gtcatgcgcc gcgtcctgat gttgatgatg gtttttcaac cagttctcga
    57421 actgtgcctg ccagtgttgt aactccatac gcgctccgtt gccaaagagt cgcaacctta
    57481 gcagttttta ttcatagcgt ctgcaacgtg cgggacgagg tcgggtgtac catgcaatgc
    57541 cgcctaacaa ggcaccaacc agggccataa acagaaatcc gaccaatgct cctaaccact
    57601 tccccgcggt agatcctaaa tcactttcaa atcccgacac gggggcatcg gggatccggc
    57661 tcatcaccca gatgtatcgc gccattgccc agacgaaata gccacaccat gtatagaaag
    57721 cccacaatga atcgccacgg ataatctaga cacttccgag ccgttgataa tactggtttt
    57781 catattctgt cggtgacatc tgttcgctag aaccatgccg acgcttactg ttataaaaca
    57841 tttcgatgta atcaaaaata tcactgcggg cttcttcccg cgttccgtag atctttttct
    57901 ttatccgttc acgtttcaac aactggaaaa aactttctgc aaccgcatta tcatggcagt
    57961 taccgcgacg gctcatgcta ccctccaggc cgtgtgattt caggaacgac tgccactcat
    58021 ggcttgtgta ctgactgccc tgatccgaat gaaccagcac ctgtttttcg ggattacgcc
//
LOCUS       DAEEGA010000016        58047 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEEGA010000016 DAEEGA010000000
VERSION     DAEEGA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58047
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.016"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(24..278)
                     /locus_tag="KLH46_001019"
     CDS             complement(24..278)
                     /locus_tag="KLH46_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBE0377793.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="KLH46_001020"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="KLH46_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBE0377794.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="KLH46_001021"
     CDS             1228..2694
                     /locus_tag="KLH46_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBE0377795.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="KLH46_001022"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="KLH46_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBE0377796.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGMLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="KLH46_001023"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="KLH46_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBE0377797.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="KLH46_001024"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="KLH46_001024"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBE0377798.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="KLH46_001025"
     CDS             5709..7265
                     /locus_tag="KLH46_001025"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBE0377799.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILGSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="KLH46_001026"
     CDS             7347..9161
                     /locus_tag="KLH46_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE0377800.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="KLH46_001027"
     CDS             9162..10256
                     /locus_tag="KLH46_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBE0377801.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSTFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="KLH46_001028"
     CDS             10256..11281
                     /locus_tag="KLH46_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0377802.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="KLH46_001029"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="KLH46_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBE0377803.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="KLH46_001030"
     CDS             complement(12876..13220)
                     /locus_tag="KLH46_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377804.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="KLH46_001031"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="KLH46_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBE0377805.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFYIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="KLH46_001032"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="KLH46_001032"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBE0377806.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="KLH46_001033"
     CDS             15616..17376
                     /locus_tag="KLH46_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBE0377807.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="KLH46_001034"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="KLH46_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBE0377808.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="KLH46_001035"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="KLH46_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBE0377809.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINYADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="KLH46_001036"
     CDS             19113..19340
                     /locus_tag="KLH46_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBE0377810.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="KLH46_001037"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="KLH46_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBE0377811.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="KLH46_001038"
     tRNA            21195..21271
                     /locus_tag="KLH46_001038"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="KLH46_001039"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="KLH46_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HBE0377812.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="KLH46_001040"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="KLH46_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBE0377813.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="KLH46_001041"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="KLH46_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBE0377814.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="KLH46_001042"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="KLH46_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBE0377815.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEKIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="KLH46_001043"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="KLH46_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBE0377816.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="KLH46_001044"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="KLH46_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBE0377817.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="KLH46_001045"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="KLH46_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBE0377818.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="KLH46_001046"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="KLH46_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBE0377819.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="KLH46_001047"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="KLH46_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBE0377820.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="KLH46_001048"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="KLH46_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBE0377821.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTTIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="KLH46_001049"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="KLH46_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBE0377822.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="KLH46_001050"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="KLH46_001050"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBE0377823.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="KLH46_001051"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="KLH46_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBE0377824.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="KLH46_001052"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="KLH46_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBE0377825.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="KLH46_001053"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="KLH46_001053"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBE0377826.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="KLH46_001054"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="KLH46_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBE0377827.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="KLH46_001055"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="KLH46_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBE0377828.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="KLH46_001056"
     CDS             37391..37555
                     /locus_tag="KLH46_001056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377829.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="KLH46_001057"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="KLH46_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBE0377830.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38328..39347)
                     /locus_tag="KLH46_001058"
     CDS             complement(38328..39347)
                     /locus_tag="KLH46_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HBE0377831.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQPVQKEV
                     ADIAL"
     gene            complement(39581..39940)
                     /locus_tag="KLH46_001059"
                     /pseudo
     CDS             complement(39581..39940)
                     /locus_tag="KLH46_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40158..41801)
                     /gene="yojI"
                     /locus_tag="KLH46_001060"
     CDS             complement(40158..41801)
                     /gene="yojI"
                     /locus_tag="KLH46_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBE0377832.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41877..42527)
                     /gene="alkB"
                     /locus_tag="KLH46_001061"
     CDS             complement(41877..42527)
                     /gene="alkB"
                     /locus_tag="KLH46_001061"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBE0377833.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42527..43591)
                     /gene="ada"
                     /locus_tag="KLH46_001062"
     CDS             complement(42527..43591)
                     /gene="ada"
                     /locus_tag="KLH46_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBE0377834.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43665..44720)
                     /gene="apbE"
                     /locus_tag="KLH46_001063"
     CDS             complement(43665..44720)
                     /gene="apbE"
                     /locus_tag="KLH46_001063"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBE0377835.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44832..45953)
                     /gene="ompC"
                     /locus_tag="KLH46_001064"
     CDS             complement(44832..45953)
                     /gene="ompC"
                     /locus_tag="KLH46_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBE0377836.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46691..49363
                     /gene="rcsD"
                     /locus_tag="KLH46_001065"
     CDS             46691..49363
                     /gene="rcsD"
                     /locus_tag="KLH46_001065"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBE0377837.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49380..50030
                     /gene="rcsB"
                     /locus_tag="KLH46_001066"
     CDS             49380..50030
                     /gene="rcsB"
                     /locus_tag="KLH46_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBE0377838.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50230..53079)
                     /gene="rcsC"
                     /locus_tag="KLH46_001067"
     CDS             complement(50230..53079)
                     /gene="rcsC"
                     /locus_tag="KLH46_001067"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBE0377839.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TVMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53354..54130)
                     /locus_tag="KLH46_001068"
     CDS             complement(53354..54130)
                     /locus_tag="KLH46_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBE0377840.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54135..55784)
                     /locus_tag="KLH46_001069"
     CDS             complement(54135..55784)
                     /locus_tag="KLH46_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBE0377841.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     AKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGKTGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55785..56344)
                     /locus_tag="KLH46_001070"
                     /pseudo
     CDS             complement(55785..56344)
                     /locus_tag="KLH46_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56503..>58047)
                     /locus_tag="KLH46_001071"
     CDS             complement(56503..>58047)
                     /locus_tag="KLH46_001071"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBE0377842.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14200 a        15659 c        14263 g        13925 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg catgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactgggta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcacattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg taaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctactg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcataa ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ctttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggtc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagagta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgaact ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatcttt tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gtcaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgtgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatccg gcataccatg ccggatgtgg cacatcatta caacgcaata
    38341 tccgccactt ctttttgaac gggttgcggt ttcggcgtac tatctgctgc ttgcggtttg
    38401 tcgtatttca gccccagcac ttcgctggtg tactgcaact cgcgttctgt tgccgctaca
    38461 tcaccgttca gcttgcgtcc ataagacgga acgatcgctt tcaacgtagc ctgccattgc
    38521 gggctggaaa cgcgatcgcc aaatactttt tccagtagat tcaacataat cggcgcggcg
    38581 gttgacgccc ctggcgatgc ccccagcagc gccgcaatgg tcccttgctg gtcactgacc
    38641 acttcggtcc ccagacgcag tacgccacct ttctcggcat cacgcttgat aatctgcaca
    38701 cgctgccccg cttgccacaa acgccagtcc tcttttttcg cttgcggata gtactctttc
    38761 aacgcttcaa aacgatcctc ttcactcaac atcacctgac tcaccagata tttcaccaga
    38821 tcgaaattat ccagcccgac gtgcatcatc ggcatcacgt tagaggtggt ggtggaactc
    38881 atcagatccc acaatgaacc gtttttgagg aatttggttg agaaggtggc aaatggccca
    38941 aacagcacta cgcgtttacc gtccagaaca cgggtatcga tatgcggaac cgacatcggt
    39001 ggtgcgccaa cggatgcttt accgtaaact ttcgccagat ggcgattaac cacgtctgga
    39061 ttttccgaaa caaggaactg tccgcccacc gggaaaccgg cgtagtcttt cgcttccgga
    39121 atccccgatt cctgtaacag cttcagcgcc gcaccgcccg cgccgataaa gacaaatttg
    39181 gcgcggatgt tctgtgcagt gccgtttttc agatcggcaa cggtaacggt ccaggtgtta
    39241 tcgtcattac gctttagggc gcggacttcg ctgctgagtt gcagcgagaa gttagatttc
    39301 ttctgtaagg aagcaattaa ctggcgggtg atctcaccat agttcacatc agtgccaatt
    39361 tctgttgcgg atcgcgccct tccatcacta acggtgccca ctctttgatc tgcgcgtgat
    39421 cttcagagta acgcataccg cgaaacagcg agctttgttg caacgcggcg taacgggcgc
    39481 gcaggaaatt aacgttatcc tcgccccaga caaagctcat atgcggaacg gtattgataa
    39541 atgaacgcgg agtacgcagc acgccgcgct cgacctggtg cgcccagaac tggcgggaaa
    39601 tctgaaatgc ttcgttaatg gcgactgctt tttcaatact gatgctgcca tcggcgtttt
    39661 gcggggtgta gttcagttcc atcagtgcag aatgcccggt tccggcgttg ttctagccgt
    39721 tcgaactctc ctgcgcgccc ccctccaggc gctccaccat ggtcatcgac cattcaggct
    39781 ccagctcgcg tcaataggtc cccaacgtgg cgctcataat gccgccgcca atcaacagta
    39841 catcagtttc ctgctcctcg gacgctttcg cttttgccgc catcgaaacg gcattaagcc
    39901 ccacggccat cgagaagagc atggcagtca cttttttcat cttgttaatg ccttactttt
    39961 agtcgcttta ttgcaggtga gatttgcgcg gcatcaacgg taacacttaa gtaacaacat
    40021 ttaaataatg tttataaatt atattctaat tttagaaaca ttataatttc gttgattaat
    40081 tatagggtta ttagaaatga agggattttt agcatattga cctgacggca gcagtccgcc
    40141 aggtcagcgt cgtaatatta tgccgtccgg gcaacggcat cacgcgaagc ggcatcgcgc
    40201 tcttcgcccg tcagctcgct aagttgccca ttgcgcattt ccagcaggcg gtcggcgtgg
    40261 ataaagtaat gatcatcatg actgatagcg aaaatagttt tacccatctc ctgcatcagc
    40321 ggcagcaaca cctgataaaa ctcacgacgg aagtgtggat cctgatccac cgcccattca
    40381 tccagcagga taatatcgcg ttcttctgcc agcgccagca acagcgccac gcgttttttc
    40441 tgcccttttg ataacttcag gttaacaata cgcccgttgc ttaactcaag cttatgagcc
    40501 attttcagct gcgccagcca cttctcaacc agttgcgggt tagcgggttg cccctccggc
    40561 cccagcagtt gatcaaacag ccagacatcg gtaaacactg ccgaaaacag tttgcgataa
    40621 tcttccggtt gttcgccgct gacaggtttg ccatccagca agatttcgcc gctttgtggc
    40681 tgatacaagc ccgtcaacaa catcgccagc gtcgattttc cgctaccgtt accgccaatc
    40741 agaaacagca gctcgccacg tttgatggtg agattaatcg gaccaacgga aaacgcgtta
    40801 tcctgataag caaacgtcac gttacgcagc tccagcgttt gccagttggg aaacgcctgc
    40861 gggcgcggaa actcagcttt gaaaggcgcg agcgcgaatt tgttcagctt gttaaacgcc
    40921 acctgcgccg tcagcagcgt cggcaatgcg ccaaccgccg aaagcagcgg cgtacgcagg
    40981 aataacagcg tcaacgaata ggtcgcagca acgttggtat cagcccaacc gagactgttc
    41041 gccatccaga acaccaggcc gatagcgccc agcatcatga tgtttgacca gttcacggca
    41101 ctaagatgga aggtgtctgc gcgaataata tgatggcgat actcttgcgc atcggggatg
    41161 tagaggttgt taaacacata ctcggcgcgt tcccggttca gagtcagctc tttgcgccct
    41221 tcaagtacag tttgaaaatc cgtgtacagc ttgtcttcgg tttcacgtag ggtcgccata
    41281 tgtttgtaca cccgcgccac cagcacaaaa ccgccccaga tggtgatcgc catccagata
    41341 gccgttacca gcaacatttt gcccgacagc atccacagat acgccgatga accgatagtg
    41401 agaatgatcc cctgccccag ttccggcaga cgcacaaaag caatggtgat attgcgcaca
    41461 tcgctggtta atcccgccag caacgaggcg ctaccgagtt gttcaatgcg ctcgacgtgg
    41521 gtatccagaa tccgcttgat aaattcacta cgcagtcggt aaacgaagtg atgccccaaa
    41581 gtggtgagcg ccagttgcga tccgagagtg actgccatca acagcagcaa taatcccaga
    41641 aactccggta acaccagcag actggtatcc gccgtttcga taaggcgctg attgataaaa
    41701 gcaattaagc caatgcctaa tgccgcactg gcgaggctta gcgccatcac actgataaat
    41761 ggccagcgat actgccgcca gacaagtaca agaagttcca tgcagaaaac ccggacaatg
    41821 aattacagcc cgcagtttaa acatcttcgc gcgcacagca ataataattc ttatttttat
    41881 tcttttttac ctgcctgacg gaatgtcagg ttgtagcggc agtcggtggt gagtggatga
    41941 aaccccgctt tcaacggttg aataccgtga taaaacagcc gcgattcacc gccccatacc
    42001 accacatcgc catgttccaa caacaaacgt ttgagcggat cattacgttt caggccacca
    42061 aattgaaaaa tcgcgggtaa gcccagagaa acagaaacaa ttggcgcgcg cagatccggt
    42121 tcgtctttat cctgatgcag cgacagtttc gcgccaggag cgtagcggtt gataagacaa
    42181 gcatctggct ggaaatctgg atagcccgcc gccgtagccg cacgttgaca taaatcatga
    42241 aagctttgtg gcatggcggg ccacggttta tttgtttgcg gatcaatggg cgaatagaga
    42301 taaccttgcc gatgggtcgt ccagcccaga tgcccacagt tggtcatcgc caccgacatg
    42361 gtatatccgc ctggagcgac catctggcga aacggcgact ggctggcaac gtcattaata
    42421 tcgcggatta gttgctccgc agcattaaaa gcaaaacgcc gtaaaattac cgcaccagcc
    42481 gccagtggct cttgccacgg ttcagcatcg gcaaacagat ccaacattac ctctcctcat
    42541 tttcagcttc gcggcgcagc agttgcgctt tacgcgacac gccccagcgg taaccggaaa
    42601 gtgtgccatc accacggacc actcgatgac agggtattac gatagccagc ttgttggcgg
    42661 cacaggcgct ggcaacggcc cgtaccgctt tcggtttgcc gatggcgtta gccagttgct
    42721 gataactgac ggtttcacca caaggtatcg tgcgcagtgc ctgccagact tgttgctgaa
    42781 aagcagtgcc gcgaatatcc agcggtaacg tcagcggcgt atcgcgttga ttgaggctgg
    42841 cgatcacttc acgcacatgt tgctgaaacg tcagatcggc aggcgcgttg tcggcagcgg
    42901 gaaacatctg ctgcaactcg ctgattaatg tcgcgtcatc atcgcccagc aatatcgcgc
    42961 aaatcccccg ctcgctttct gccaccaggc aacgacccag ctcacaatca gccagcgcgt
    43021 aacgcaccgc cagattttcg ccaccgtgac ggaattgttt agccgtcatg cccagcgttt
    43081 catcggcttt gcggtagtaa ctgctgctgt cggggaatcc ggcgttaaga atagacgtcg
    43141 tcacgctctc ccctttcgcc agcgattcgc gcaaacggcg agcgcgccag gcctgttgcc
    43201 aggctttagg cgtcattccg gtagtagctt taaacaaccg atgtagatga aatggactca
    43261 tcgccacctg gtcggctaag gcttccagcg ttacaggcgt ttcctgttcc agcagtcgac
    43321 acgcgtgggt gattttatcc aaccgatgtt gccggggatt ggctttgtct ggctgacaac
    43381 gtttgcaggg gcgaaatccg gcggcgagtg cctcgctggc atttgcgtag aaggagacat
    43441 tttcccgcaa ggcatgtctg gcgcggcaag aaggacggca aaagataccc gtggtgcgca
    43501 cggcgaaaac gaattcgccg tcggcattcg ggtcgcgggc taagacagat tgccagcgtt
    43561 gatcgtcagt taagcatgtg gcgtttttca taatcagctc cctggttaag gatagccttt
    43621 agtctgcccg gtcaccatca cgcaaaaacc aacaatcttg cgctttaatt tttttcgctg
    43681 acaaggaagc ttttaaactg tggtgacatc caggttttaa agctatcgcc ttctttggtg
    43741 atcatataaa ccgccagccc ttcccggcga acaacctctt tggctttctc cggcccgagt
    43801 accatcaagc cagtatccca ggcatcggct tccagcgccg tcggagcaat caccgtcacg
    43861 gataccagat tgtgttcgat gggacgcccg gtttgcggat cgataacatg ggaaagacgt
    43921 ttgccgtcca gttcgtaata gttacggtag ctgccagagg tgctaatccc atgaccgttg
    43981 atatccacca cagcctgaac cgcgttttct ttatcggttg gtttttgaat cgctacccgc
    44041 cacggctggc cttcaccgtt cataccacgg ctgttcagcg cgccgcccac cgacaccaga
    44101 tagcgggaaa tcccttcctg ctccatcaag cgtgccaggt gatccgccgc ataaccttca
    44161 ccgacggtgg agagatcgac atataaatcc ggcaggtctt tttgcagata ttgctgatgc
    44221 gactgattga tgaccgtcag gtgctgtaag ccggttttgg ctttcatcgc atcgatctgt
    44281 tcctggctcg gaatttgaac cggctgttgt tccgggccaa agccccacag attcaccagc
    44341 ggcccgacgg taatatccat cgcaccatcg gtccttgccc caatgcgcag cgaggtggtg
    44401 acgatatcgg ccatcgcttc acttaccggc cacggcgaca aactttgcga gtcgttaaag
    44461 cgcatcagcg cggaatcttt tttataggtc gaaagcagct gatcgtcggc gtccagctgg
    44521 gtctgaatct tttctttaag ttcggcactg cgtttggcat caatgcccgg gatgctggca
    44581 cgccagaagg tacccatggt tttgccttca agaacggtaa cttcagtagc gtgggttttg
    44641 gcgggttgtg gtttttgatc gcaaccaaca aagaagagcg cggcaaccag cagtgccacg
    44701 cgggtaaagc ttatttccat acgtgattat cctcatgcga acggtcgcaa gagtacacca
    44761 aaaaactgtg tttgtacgct gaaaacaatg aaaaaagggc ccgcaggccc tttgttcgat
    44821 atccatcgag attagaactg gtaaaccaga cccagagcta cgatgttatc agtgttgatg
    44881 ccagcgtcac gagtgaactg gttgtcgtcc agcaggttga ttttgtagtc aacgtaggtg
    44941 gacatgtttt tgttgaagta gtaggtcgca ccaacatcaa catatttcag gatatcttcg
    45001 tcgtcgtagt tacgaccatt gatgacaccc aggtttttac ctttagactg caggtaagcc
    45061 agggacggac gcagaccgaa gtcgaactgg tactgagcaa cagcttcgaa gttctgtgct
    45121 ttgttcgccc aacccaagtt gcctacgcga gttgcgttgt aggtctgggt gtactgagca
    45181 gctaggtaga tgttgttcgc gtcgtatttc agaccaccgg tgtaggtttc agcgcggtcg
    45241 ccattaccga tgtaacgctc atcataacgg ttcagaccga agttctggtc atcagtacgt
    45301 ttggagctgg aaactgcagc accgataccg aagccttcgt aatcataagt aatagaaccg
    45361 ccaacgccgt caccgttctg acgcagagct tcacgaccgt tgttggtcat gccttcacct
    45421 tccgggctac cgtttttgcc ctgatactga acagcgaagt tcagaccgtc aaccagacca
    45481 aagaagtcgg tgctacggta ggttgcgaag ccgttaccac gctgctgcat gaagttgtca
    45541 gaaccgtaag tgtcaccacc gaattctggc agtacgtcgg tccaggaagt tacgtcgtaa
    45601 actacgccgt agttacgacc gtagtcgaaa gaacctacat cctggaattt cagacctgcg
    45661 aatgccacac gggtccagga gttgttttcg ttttcagcgc tgttgccctg gatctgatat
    45721 tcccactggc cgtaaccggt cagctggtca gtaacctgag tttcaccttt gaagccaaga
    45781 cgcatgtagg tctggtcgcc atctacagac ttgtcgtcag agaaatagtg caggccgtct
    45841 actttaccgt acagatctaa tttgttgccg tctttgttgt aaacttcagc agcgtttgct
    45901 gcgcctgcta ccagcagagc tgggaccagg agggacagta ctttaacttt catgttatta
    45961 accctctgtt atatgccttt atttgctttt ttatgccact gcatactgat taaccctcat
    46021 taatcagtcg gcaagtccat tctccccaaa aatgcagaat aatccaacac gaatatgata
    46081 ctaaaacttt taagatgttt catttatcgc tatagatgtt tcaaaatgta aatacaaggg
    46141 aactttttaa gattattgcg gaatggcgaa ataagcacct aacatcaagc aataataatt
    46201 caaggttaaa atcaataact tattcttaag tatttgacag cactgaatgt caaaacaaaa
    46261 ccttcactcg caactagaat aactcccgct atcatcatta actttattta ttaccgtcat
    46321 tcatttctga atgtctgttt acccctattt caaccggatg cctcgcattc ggttttttta
    46381 cccttcttta cacacttttc attattctgt gctaccacag aaaaactata acgcttgtta
    46441 actatttcac aaataattaa catcagcata atttccagca atctttgttt atttgcaatt
    46501 aatttcgttg ggctttttgt aggttatttg tacagcaaaa tggcgcttgt acatctattt
    46561 ccccccatac aggatgataa atatcacggg agaatagaga atcatcaatc aggtaagagt
    46621 ctggaatttc acactgtacc ctttatactg ccctatcact tcgcgaagtt ttaacaggtc
    46681 ataaacacga atgcgtcaga aagagacaac ggccacgacc cgcttttcac tcctaccggg
    46741 gagcattacc cgcttctttt tactgttgat cattgtgtta ctggtgacga tgggggtaat
    46801 ggtacaaagt gccgttaacg cttggctgaa agataaaagt taccagattg tcgacattac
    46861 ccacgcaatc caaaagcgcg tcgatacctg gcgttacgtg accaggcaga tctacgacaa
    46921 cattgccgcg acgacctccc cctcctccgg cgaaggttta caagagacgc gcctgaaaca
    46981 ggatgtctac tatctggaga aaccacgccg caaaacggaa gcgttaatct ttggctctca
    47041 cgacaactca acgcttgaga tgactcagcg gatgtccact tatctggata cattgtgggg
    47101 tgcagaaaat gtaccgtggt cgatgtatta cctgaatggt caggataaca gtctggtgct
    47161 gatctcaacc ctacccctta aagatctcac ctccggattt aaagaatcga ccgtcagtga
    47221 cattgttgat tcacgtcgtg cagaaatgtt acaacaggcc aacgccctcg atgaacgcga
    47281 aagtttttct aacatgcgcc gcctagcctg gcagaacggt cattacttta ccttgcgtac
    47341 cacgttcaac cagccgggac atctggcaac ggtcgtggct tttgatctgc cgattaatga
    47401 tttgatccca ccgggtatgc cgctggacag tttccgcctt gagccagacg cgacggcaac
    47461 gggaaacaat gataatgaga aagaagggac ggatagcgtc agtatccact ttaacagtac
    47521 gaagattgaa atctcctcgg cactcaactc taccgatatg cgcctggtct ggcaggttcc
    47581 ttatggcacc ttattgctgg atacgttgca aaacattctg ctgccactgc tgctgaacat
    47641 cggtttgctg gcgctggcgt tatttggcta taccacattc cgccatttct ccagccgcag
    47701 tacagaaagc gtccccagca cggcggtcaa taacgaattg cgcattttac gggcaatcaa
    47761 tgaagagata gtctcactgt tgccgctcgg cctgctggtt cacgatcagg aatcgaaccg
    47821 cactgtcata agtaacaaaa ttgccgatca tttgctgccg catctgaatc tgcaaaacat
    47881 caccaccatg gcggaacagc atcaggggat tattcaggcg acgatcaata acgagctgta
    47941 tgagatccgc atgttccgca gccaggtcgc gccgcgcaca caaattttca ttattcgcga
    48001 tcaggatcgc gaagtgctgg taaacaagaa actcaagcag gcgcagcgtc tgtatgagaa
    48061 aaaccagcag gggcggatga cctttatgaa aaacattggc gatgcgctga aagaacccgc
    48121 acagtccctg gcggagagtg cggctaaact caacgccccg gaaagcaaac aactggcgaa
    48181 tcaggcagat gtgctggtgc ggctggtcga agaaatacag ttagcgaaca tgcttgcgga
    48241 tgatagctgg aaaagtgaga cggtgctgtt ctccgtgcag gatttaattg atgaagttgt
    48301 gccttcagtg ttgcctgcca tcaagcgtaa aggtctgcaa ctgctgatta acaatcatct
    48361 gaaagcacac gatatgcgcc gcggcgatcg cgatgcctta cgacgtattt tgctgctact
    48421 gatgcaatat gccgtgacct caacgcaatt gggaaaaatc acccttgagg ttgatcagga
    48481 tgagtcctct gaagaccgcc tgacattccg cattctggac acgggagaag gcgtaagtat
    48541 tcatgaaatg gataatttgc acttcccgtt tatcaaccag acccaaaacg atcgttatgg
    48601 aaaggcggac ccgctggcat tctggctgag cgatcaactg gcacgtaaac tgggcggtca
    48661 tttaaacatc aaaacgcggg atgggcttgg tacacgctac tctgtgcata tcaaaatgct
    48721 cgccgctgac ccggaagttg aagaggaaga agagcgttta ctggatgatg tctgcgtaat
    48781 ggtggatgtt acttcggcag aaattcggaa tattgtcact cgcctgttag aaaattgggg
    48841 tgcaacctgt atcacacccg atgaaagatt aattagtcaa gattatgata tctttttaac
    48901 ggataatccg tctaatctta ctgcctctgg cttgctttta agcgatgatg agtctggcgt
    48961 acgagaaatt gggcctggtc aattgtgcgt caacttcaat atgagcaacg ctatgcagga
    49021 agcggtctta caattaattg aagtgcaact ggcacaggaa gaggtgacag aatcgcctct
    49081 gggcggagat gaaaatgcgc aactccatgc cagcggctat tatgcgctct ttgtagacac
    49141 agtaccggat gatgttaaga ggctgtatac tgaagcagca accagtgact ttgctgcgtt
    49201 agcccaaacg gctcatcgtc ttaaaggcgt atttgccatg ctaaatctgg tacccggcaa
    49261 gcagttatgt gaaacgctgg aacatctgat tcgtgagaag gatgttccag gaatagaaaa
    49321 atacatcagc gacattgaca gttatgtcaa gagcttgctg tagcaaggta gcctattaca
    49381 tgaacaatat gaacgtaatt attgccgatg accatccgat agtcttgttc ggtattcgca
    49441 aatcacttga gcaaattgag tgggtgaatg ttgtcggcga atttgaagac tctacagcac
    49501 tgatcaacaa cctgccgaaa ctggatgcgc atgtgttgat taccgatctc tccatgcctg
    49561 gcgataagta cggcgatggc attaccttaa tcaagtacat caagcgccat ttcccaagcc
    49621 tgtcgatcat tgttctgact atgaacaaca acccggcgat tcttagtgcg gtattggatc
    49681 tggatatcga agggatcgtg ctgaaacaag gtgcaccgac cgatctgccg aaagctctcg
    49741 ccgcgctgca gaaagggaag aaatttaccc cggaaagcgt ttctcgcctg ttggaaaaaa
    49801 tcagtgctgg tggttacggt gacaagcgtc tctcgccaaa agagagtgaa gttctgcgcc
    49861 tgtttgcgga aggcttccta gtgaccgaga tcgctaaaaa gctgaaccgc agtattaaaa
    49921 ccatcagtag ccagaagaaa tctgcgatga tgaagctggg cgtcgagaac gatatcgccc
    49981 tgctgaatta tctctcttct gtgaccttaa gtccggcaga taaagactaa tcatctgtag
    50041 gccagataag acgcgttagt gtcttatctg gcatttgcac cgattgccgg atgcggcgta
    50101 aacgccttat ccggcctacg attcccatta tttcaacaga ttacattaaa gtaggccaga
    50161 taagacgcgt cagtgtctta tctggcattt gcactgaatg ccggatgcgg cgtaaacgcc
    50221 ttatccgtcc tacgaatccc gcgatttcct gaccctctcg gcatataccg tcagcgtctg
    50281 ttttatcaca tccagcgtta ccggcttcga gaggcagctg tccataccgg actccagaca
    50341 ccgctgcttc tcttcagcca acgcattagc agttactccg attaccggca acgtcagtcc
    50401 caactgacga atgcgttgcg tcaagcggta accatccata tttggcatgt tgacgtcgct
    50461 aagcacgata tcaatatgat tcttactaag tacattaagc gcatcgacgc catcattcgc
    50521 ggttttacat tgatagccca acgatcccaa ctgatctgcc agcagacgtc ggttaatcgg
    50581 atgatcatcc acgaccagaa tcatcatatc gtcgttatcg ctgaccgctt tgtccgtcga
    50641 cggcagagcg ttagcaggat cgtcgctctc catctcgatc aaataaatac gcgccaacaa
    50701 tgccggtagc tcatgcggag cagccacact gtgtacccac tcccctggcg ctttctccag
    50761 cggaatacca atatggcgac gacagaaggt cactaccgct ctgccctgcc attttttact
    50821 cactacctcg tcagtgatta acacatcttc gggagtcggt tcctgccctt cgtatgttgt
    50881 aacgacgatg ccgctgcgct gcaaactggt ttccaggaac tgacagagcg acgcattgcg
    50941 gaccgccagc cagcagcgtt taccactcaa cccttccacg ccttttttct gcgggtactg
    51001 agcgccgtac aacggaatac gcacggtaaa ctggctgccc attcccggtt ctgaatctac
    51061 cgagatatcg ccgtccatca tgctgatcag tttttcacaa atcgccagac ccagaccggt
    51121 cccctggaag tttcgctgta cgcccgttcc aacctggaag aagggatcaa acaagcgcac
    51181 cacttctttc gccggaatcc ccacgccggt atcgcgaaca cggatagaga gataatcgcc
    51241 atcagcgcga acatgcaaaa ctatacagcc ggtatcggtg aattttatgg cgttactcaa
    51301 caggttggag atgacctgct gtaaacgcat cgggtcgcca tttaaggcca ctggcacatc
    51361 cggttcaata aagcagtaca agcctaactg cttgcgtacc accagcggta aatagttggc
    51421 ggtgatgtgg ttcatcactt cacgcggtga aaactcacgc ggttcgatct tcaactgttc
    51481 cgattcaatc ttcgagaaat cgagaatatc gctgataatt ttcaacaaca ggctggaaga
    51541 gttgttcatt accgtcacca gacgatcgac gcctttcggt aactctttgg tttgcaacag
    51601 atccaggtta ccgataatgc catacagcgg cgttcgcagc tcatgactga cggtggcaag
    51661 gaacatcgat tttgactggc tcgcctgttc cgctgcttgt gccatctcct gcaacgactc
    51721 ttccatcttc acgcgcgaag aaacatccac cagcacacaa atggccacgt tttcattacg
    51781 atagcgcgaa tggacgaagc tgatttgcag attggtattg ttgctggtca ggacatcaac
    51841 aaaattgacc tgctgcccac agataatttg cgtcagtcgt tggcggtcct catgcgtaag
    51901 catattgaga taggtatgcg ccagttcgtt acttaaaata ttgacgccat cagcggtacg
    51961 caaaatgcag atacccactg gcgcggaggc gacaatcttg cgattgaact gctcatgttc
    52021 ttccagtcgc agggcgtcgc tttccgccgg aatgaaaata cgtcgctcgt acatccgcgc
    52081 gagagtaaac aatgcagctc cggcaagcac attcagcaaa attgcgttaa ggatcaacat
    52141 gcgaatgcgt tccagcacct tatcaaccgg caccgaatac acgatgctta gcgatgaggg
    52201 tggcagattt ttcttcagca ccagctcccg gaacccttcc gtatagccaa accaggagcg
    52261 ttcctgcatc cagcgaggat cgcccttaat tttactttct ggtccggtaa gcgaaatcag
    52321 ggtatgacca ttttcatcaa gaatggtaac ccccatcggc aacgtacccg gtaagaaaaa
    52381 gttctccatc cggatggtct gctcgacacc caaaagcgcc tgcaaccggt tcgccagata
    52441 aactggcgtc aacgcgtaaa aatacccgac gcaagggcgc ggaccttcgc tgatccagta
    52501 gaggttactg ccgctatcat cttgtggtgc atttcgatat ttattgatgc gttcatgcaa
    52561 cgctttcaac gcggtatcgc gttccactgg catatcacgc agaccgaaat tggccatgca
    52621 gaggttatca ctgccgatta aaaatacccg gttgagatcg taagccgcag aaaaattatc
    52681 gcgccagtag cgcataaacc acgccaatga ctccagagaa cctcgccagg tgttactcat
    52741 tgcggaacaa tcggagtcgg caaacagcgg ttcaaacgca ggcacatccg cctgcgtttc
    52801 tcgtccacgc ggggaaagca caccgttttc tgccgataag cgattttcgg cgatgtactt
    52861 aagctctttc atcacatcag aggtgcgttg aataaagcgc tgagcctgat cggaactcag
    52921 attaaattcc tgacgaattt ccgattctcg ctgatgtaac gcattaacga tgtaaaaaac
    52981 ggatgaaaaa gcaatcaaca gccagagcac taacgccaat gctctgaaca tgtagcgcga
    53041 ggctttcagg gttgtacgaa aagaagcaag gtatttcaaa ggggagaagc tccgcctcag
    53101 gtgaccgatg gagtgtggtt aaggtagcgg taaaagcgtg ttaccgcaat gttctgtcac
    53161 cattgcaata atgcgtatcg cttagaaacc attgccttaa ccaggaaagc cggagtagat
    53221 ggacagttct cagtacgaaa aataagaaat gaccaatctg ccgacgcttg ttaatggtta
    53281 tatcctggcg tcggcagatc gcccagcagt cttatctgaa aaaagagaga gccaggttgt
    53341 gtgaagggat ggatcaccag tcaaaacttt tcaccagcgt cagctcgcca gcattacgca
    53401 tcggtacaat aaatgtttcc tgtttctcat tgaccgatcc ttcatcggtg atcagcgtca
    53461 gttgggcggt cgtcaattcc gtttcgctgc gcccaccata gtagttgata tacacctgat
    53521 agcgcccgtg aactggtgct ggcatggcga aaatctcggg tccgtacccc gtcgtgacat
    53581 ccatatccag tgcaccactg tttttcagca cggtgttacc gtaccaggcg tgttcgccat
    53641 cgggcgtgac aacatggaga tcgagatcgg tattgtccgt atcccacgag agaaccagcc
    53701 gtaaacgtgc acgaatagtt cctgtacccg gcgttgagta aaactgcatt ttttgccggc
    53761 tttgcccatc cgggctgatg acctgcacgc tgttgctgcc ttcagtgaaa atataagggc
    53821 gtgcaaatga accatcggat tcaattctct gcggcatact ggcaccgttg accaccagcc
    53881 ggccctgctg aactttaccc gccgcagcat aattctttat tttgccgcgg atctgcgctg
    53941 agatattttg atcatctgcc atgttgacgg acgacgccgg gtaattgatc gattgtgaaa
    54001 aactggcatc ttcgccctct gccggatgcc agccagagag cggtgcgtca atctcgactt
    54061 cctgcacacc ttcactgtga gcgacaggcg aaagtgccac cagtaacaac ggaagaaaaa
    54121 tctttcgcat agcattagtc cattaataat tgtcgggtca gcgtttcgat atagttctca
    54181 tcaagcccgg tgggataacc atcaaaggcc aagtgcagat actcatgggt taaatccagc
    54241 cgatcctgaa gcgtaaagaa gttacgaata aacagccgtt tctgctgccg atcggtatag
    54301 gggaaacctg agactaaacg acagaccgca aacacgtctg gttcgttgta ccccgtcttg
    54361 ccttgtaata tacgccgcca ctgcggcatt tttttcgcca gccaggcttt cgctttgggt
    54421 aataactggc aggttgagcg tggagctccc cagcggctaa ggctgttgtc gggataagca
    54481 aacgcgagta tctggtcgta acgctccccc tgccccgcct gggccgtggc ttgccgccag
    54541 gaaagtgtcc cttcggtagc gcgactgcca tgataatgaa ccggatcgcc tgcatagatg
    54601 agctcctgcg tccaggcggt catcgttcgc gcccccgtgg tcgctggcga agcagaaaca
    54661 cgctgcgtgg cgctgctgtc ggggatggtc aggcaatcac cttcccgatt ggcgttttgc
    54721 tgtaaaaaag tacgtatcgc cacagtcatg gctttcgctg cctcaggtgg cgtacttttc
    54781 gcctcgcgat ccagaactct ggtaacgtac tcttcacgat cgagatgcga ctgcaatttc
    54841 agtttgccct tttcgctcag taaggtcgtc tcaccgtggc tgacaaacgt aatgtggtta
    54901 ccattagtaa acgtcacacg atagcgccca ttaagtacgc tgggttttac tgccgtcgta
    54961 ctcttttccg ctgtgatttt cttcagcgga tagcgggcaa acagctccac ctctacacat
    55021 tgcccactgg caacctgcgt cggcactggc aaaacccgat taagcaccgt cgcataacgg
    55081 gttagtaccg ttttgctggt gccactgcca gtaacccaca gcggtgtacc atcggttaac
    55141 cagccagcaa aaccaccctg acggatttcc tgtttatcat cggcaaacca gctccaggtt
    55201 ttcacccgta cccgactacc gagcatggat gcgacaccga ttttcgcctc gtccagcacc
    55261 acatccagta agacttcctg agctttgttt tgcgcaggta acgtcgctaa tgaatcaagc
    55321 agcgatttaa ccgttaccga ggtttctggc ttcagcgttg taagagacgc taaccacgca
    55381 ggtgcctgac gctgttgcca gtattgcccc cacacatctg cgccaatatg caaccgctgc
    55441 ggcgcaaaat agagcccaca ggaacgtacc agcgcggtat cgcgcgttat gctctcccca
    55501 gcctgacaac aataaacctc ttccggggag tttccgcgac actggtaggg ctgctccggt
    55561 tgatgggtat cctccagcca ggcgtaaaca tacagtttcc acaaactccc taacggcgtg
    55621 gtgagcgagt caggtaatgc tgagactttg gccacgcctg atgacgacag ctgatacagt
    55681 tgatcgtgct gcgcaccgcg cagcaccagt tgtaacggcg tctcctccgc aagcgttggc
    55741 agagtaacca acgccaatag ccagacgatt ctgcgccagt tcacttattt cacctgcatc
    55801 ccggtccatt cactccctgc cgcgacactt tgctgcgcag gtgcatagga acgcacataa
    55861 cgagcaggag gcagaacgaa ttgccctttt tgcgagaagc gcagcaaatg gcggaaagtg
    55921 accgttcccg tcagttcttt caccggcacc atataggcca gttcgcccat ttcattacgc
    55981 gctttttcca gcaattgccc ctgctgtttc gcggcgttgg gtttattgac tgaaatgccc
    56041 catgttgtgc gctcaacgtc ggctcccggc gggagcggta cttccacctg accatagcgc
    56101 agaactgcat cctgctcgct ggtaagcgta atttcatcga gatacagcgc atcgctgtca
    56161 atctcattgc tggtcaccgg ctgcagagta aagctcatct cttcttcacc ggggataagc
    56221 cgatacaact ggcgttcaac ggtcaccgga atgttacttt gttgagccgt ttttgccggt
    56281 tcacgccagc ggacctgcac atttgcggcg ataattcgtc accaaaagag agaatgtccg
    56341 gcacacccta accaacccaa cgccagtctt cgccccctcc agttaactta tgtttagccc
    56401 atgcgcccgc aggcgcaggc aaaacaactg gaggcattgt cgccatatat ttcgccagcc
    56461 agttcatggc cagcgcgcgc tcaatagtgg attgctcagc ggtcaaacca ttaaaatagc
    56521 ggccgtctgg gtagcgtcac caccgctatt gagcagcaca accgtgtgcg ccagcggctg
    56581 attgctgttt gccgctttat cccacgcata ttgctgaacc tgtggcatca ccgtggactt
    56641 caactgcgct tttttcagca acgtcatggt taagacgttt gccaccgcat ctgccagtgg
    56701 agattccggt gtatcgagga tcaggctgtc attgatatcg cggatgtctt cctcagagaa
    56761 atcttcatct ttagttccgc gccgggcgat agcttcatcc ccccctttca acaacgtttt
    56821 gcacggcaga ttcatctcct gtgcccacgc cagcaccagc gcccgatgca ataacggcat
    56881 gttatccgcc tgctcggcat aactgtcgag catatgctgc cagtattccg gttgttgcgt
    56941 tacgccgagc gcctggctgg cctgccagtc ggcgtaccat gcccatgccg taaggaaggc
    57001 gtcaccattg ccatcttcac cccaccaggt aaagcgcgct ccgggccccg ccagttgcat
    57061 cagccgcaga cggttatcct gaatcatctg acgaatgtcg ttagcggcgg cactttgatg
    57121 atcggcaagc gaacgccagg cgagactgag cgggatcaga cggctaccgg tgttgatcac
    57181 cccaccccac ggttcatcga ctaacgcatc aagattgttg cggaaaatct cctgcggcgt
    57241 ttcactactt tgtagccgga tattactcgc ctgctcgggc aacatcagcg cgttctcgcc
    57301 accgccgagc atgacatttt tctgctgttc aacgggccag ctgttgtcca caaaagacag
    57361 ttttgtgcta atgctgtcct gcacttgccc attttgttgc agttctgcac ttaacaagcc
    57421 agattgctga atattctgcg tcagcgaaat ataattcgcc cctttgtgca gcgtcagcgt
    57481 ctggcgcatc tcagcgcctg caaatttagt caccagcgcc accggttcgt tatcctgctg
    57541 actgaagata aacagtcctg ccgccggttt gtcgcccacg cgatacaccg ttggcatact
    57601 ccacttcatg tagagatttt tttccgaacg cagataagca cgcccctgcc cgaccagccc
    57661 gtcgccgttc atcccacgcg cggtgatacg ccagcgggtt aacgaatcag gcatcaggaa
    57721 cgtgaaatac gctttgcctt gtttatcggt tgtgagtgac ggcatccatg ccgcggtatc
    57781 cacctcttca cgccgtggac gttcaagcat ttttactcgc cgctcgctgc ggttagtcgc
    57841 gccaggcgca accggctcgc tagagagcgc ctggtcatag ctgataaacg acagactgga
    57901 gctggtacgc acattgttac gccccagcgg atagaaaaat ttgccgatat tcggcgcgat
    57961 ttctggttgc agcgcgtaga tcatttcatc gaccacgcct accgttagct gcgcagaaac
    58021 aggtttacct ttcagcgacg aggttaa
//
LOCUS       DAEEGA010000017        55428 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEEGA010000017 DAEEGA010000000
VERSION     DAEEGA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55428
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.017"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="KLH46_001072"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="KLH46_001072"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBE0377843.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="KLH46_001073"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="KLH46_001073"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBE0377844.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="KLH46_001074"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="KLH46_001074"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBE0377845.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="KLH46_001075"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="KLH46_001075"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBE0377846.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="KLH46_001076"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="KLH46_001076"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBE0377847.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="KLH46_001077"
     CDS             7826..9559
                     /locus_tag="KLH46_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBE0377848.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="KLH46_001078"
     CDS             9708..10559
                     /locus_tag="KLH46_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0377849.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="KLH46_001079"
     CDS             complement(10546..10887)
                     /locus_tag="KLH46_001079"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBE0377850.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="KLH46_001080"
     CDS             complement(10889..11767)
                     /locus_tag="KLH46_001080"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBE0377851.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="KLH46_001081"
     CDS             complement(11733..14030)
                     /locus_tag="KLH46_001081"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBE0377852.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDTYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="KLH46_001082"
     CDS             complement(14081..14401)
                     /locus_tag="KLH46_001082"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBE0377853.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="KLH46_001083"
     CDS             complement(14416..15495)
                     /locus_tag="KLH46_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBE0377854.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="KLH46_001084"
     CDS             15804..16907
                     /locus_tag="KLH46_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBE0377855.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="KLH46_001085"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="KLH46_001085"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBE0377856.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="KLH46_001086"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="KLH46_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="KLH46_001087"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="KLH46_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20983
                     /locus_tag="KLH46_001088"
     CDS             20366..20983
                     /locus_tag="KLH46_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBE0377857.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="KLH46_001089"
     CDS             complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="KLH46_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBE0377858.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22009..24189)
                     /gene="katG"
                     /locus_tag="KLH46_001090"
     CDS             complement(22009..24189)
                     /gene="katG"
                     /locus_tag="KLH46_001090"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBE0377859.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24518..25408)
                     /gene="metF"
                     /locus_tag="KLH46_001091"
     CDS             complement(24518..25408)
                     /gene="metF"
                     /locus_tag="KLH46_001091"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBE0377860.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25757..28189)
                     /gene="metL"
                     /locus_tag="KLH46_001092"
     CDS             complement(25757..28189)
                     /gene="metL"
                     /locus_tag="KLH46_001092"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBE0377861.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28192..29352)
                     /gene="metB"
                     /locus_tag="KLH46_001093"
     CDS             complement(28192..29352)
                     /gene="metB"
                     /locus_tag="KLH46_001093"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBE0377862.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29629..29946
                     /gene="metJ"
                     /locus_tag="KLH46_001094"
     CDS             29629..29946
                     /gene="metJ"
                     /locus_tag="KLH46_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBE0377863.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30130..30738
                     /locus_tag="KLH46_001095"
     CDS             30130..30738
                     /locus_tag="KLH46_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBE0377864.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="KLH46_001096"
     CDS             complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="KLH46_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBE0377865.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31214..33412
                     /gene="priA"
                     /locus_tag="KLH46_001097"
     CDS             31214..33412
                     /gene="priA"
                     /locus_tag="KLH46_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBE0377866.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33568..34593
                     /gene="cytR"
                     /locus_tag="KLH46_001098"
     CDS             33568..34593
                     /gene="cytR"
                     /locus_tag="KLH46_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBE0377867.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34685..35644
                     /gene="ftsN"
                     /locus_tag="KLH46_001099"
     CDS             34685..35644
                     /gene="ftsN"
                     /locus_tag="KLH46_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBE0377868.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35737..36267
                     /gene="hslV"
                     /locus_tag="KLH46_001100"
     CDS             35737..36267
                     /gene="hslV"
                     /locus_tag="KLH46_001100"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBE0377869.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36277..37608
                     /gene="hslU"
                     /locus_tag="KLH46_001101"
     CDS             36277..37608
                     /gene="hslU"
                     /locus_tag="KLH46_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBE0377870.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37675..38601
                     /gene="menA"
                     /locus_tag="KLH46_001102"
     CDS             37675..38601
                     /gene="menA"
                     /locus_tag="KLH46_001102"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBE0377871.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38694..39179
                     /gene="rraA"
                     /locus_tag="KLH46_001103"
     CDS             38694..39179
                     /gene="rraA"
                     /locus_tag="KLH46_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBE0377872.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="KLH46_001104"
     CDS             complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="KLH46_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBE0377873.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39935..40780
                     /gene="glpF"
                     /locus_tag="KLH46_001105"
                     /pseudo
     CDS             39935..40780
                     /gene="glpF"
                     /locus_tag="KLH46_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40820..42310
                     /gene="glpK"
                     /locus_tag="KLH46_001106"
     CDS             40820..42310
                     /gene="glpK"
                     /locus_tag="KLH46_001106"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBE0377874.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDVLGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42540..43550
                     /gene="glpX"
                     /locus_tag="KLH46_001107"
     CDS             42540..43550
                     /gene="glpX"
                     /locus_tag="KLH46_001107"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBE0377875.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43647..44393
                     /gene="fpr"
                     /locus_tag="KLH46_001108"
     CDS             43647..44393
                     /gene="fpr"
                     /locus_tag="KLH46_001108"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBE0377876.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="KLH46_001109"
     CDS             complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="KLH46_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBE0377877.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44853..45152)
                     /locus_tag="KLH46_001110"
     CDS             complement(44853..45152)
                     /locus_tag="KLH46_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBE0377878.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45364..45804)
                     /locus_tag="KLH46_001111"
                     /pseudo
     CDS             complement(45364..45804)
                     /locus_tag="KLH46_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45905..46504
                     /locus_tag="KLH46_001112"
     CDS             45905..46504
                     /locus_tag="KLH46_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBE0377879.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46612..47379
                     /gene="tpiA"
                     /locus_tag="KLH46_001113"
     CDS             46612..47379
                     /gene="tpiA"
                     /locus_tag="KLH46_001113"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBE0377880.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="KLH46_001114"
     CDS             complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="KLH46_001114"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBE0377881.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LVWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48296..49285)
                     /gene="sbp"
                     /locus_tag="KLH46_001115"
     CDS             complement(48296..49285)
                     /gene="sbp"
                     /locus_tag="KLH46_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBE0377882.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     SHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="KLH46_001116"
     CDS             complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="KLH46_001116"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBE0377883.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="KLH46_001117"
     CDS             complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="KLH46_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBE0377884.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="KLH46_001118"
     CDS             complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="KLH46_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBE0377885.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52448..53146
                     /gene="cpxR"
                     /locus_tag="KLH46_001119"
     CDS             52448..53146
                     /gene="cpxR"
                     /locus_tag="KLH46_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBE0377886.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53143..54516
                     /gene="cpxA"
                     /locus_tag="KLH46_001120"
     CDS             53143..54516
                     /gene="cpxA"
                     /locus_tag="KLH46_001120"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBE0377887.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="KLH46_001121"
     CDS             complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="KLH46_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBE0377888.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13505 a        15020 c        14159 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcagttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggt gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcagcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgtgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtaccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgaaggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctgacagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatccg gcgtatatgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg
    20581 tgtatgttcc gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact
    20641 ggttcacgaa gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg
    20701 cccggacagc aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca
    20761 caacaaaaca cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc
    20821 ctggcgatta gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga
    20881 cggtacgccg ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt
    20941 tagctggcgg atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga
    21001 agaaaaaatt cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc
    21061 cagtgccagc acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag
    21121 cagccaccag gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat
    21181 actggcaggc aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag
    21241 aatcgcactg taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg
    21301 caccagtaaa gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa
    21361 cgataataaa tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc
    21421 cccccaacta acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag
    21481 ttgccacggc tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg
    21541 cccacgtcgc aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat
    21601 ggtatagctc aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccaa
    21661 accaaccatc ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg
    21721 gcgcattccg cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca
    21781 gcgtaaggcg gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca
    21841 gctataactc cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt
    21901 tcctgcggca gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta
    21961 aagaaacttc agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc
    22021 gaaacggtcg aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc
    22081 gtgggcatcg ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa
    22141 taccagatcc gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc
    22201 gaacagctct ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac
    22261 gaagaagtca ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc
    22321 gaagttggca cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag
    22381 cgtcagttgc tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc
    22441 gcgatagtta cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt
    22501 ctgatcctga cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc
    22561 ggcgcttgcg gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga
    22621 ggctttacca gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc
    22681 gttcacatcc cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg
    22741 tttgtcgcca ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac
    22801 agacagacca gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt
    22861 gtagatcggc tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc
    22921 gatgtagcga gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa
    22981 ggcttcgttg aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg
    23041 atcaaaacgc agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc
    23101 gaacggatcc gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg
    23161 actgcgggtc tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg
    23221 ggtcggcgtc tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc
    23281 gctgccgtaa gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc
    23341 aggacctacg tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc
    23401 aatcagcgcc acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc
    23461 tgctgccgca gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag
    23521 gcccatctcg gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag
    23581 ccaggctttt tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc
    23641 ggcaccaaaa ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat
    23701 aaacaggtcg gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg
    23761 acgcgcttta tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg
    23821 accacgaccc gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc
    23881 catacgaata aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc
    23941 tgtcagcagg gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt
    24001 gcggtagtca aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc
    24061 aacacggagt tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc
    24121 gtgaccgccc tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga
    24181 cgtgctcatc aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag
    24241 aagttataat tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa
    24301 agagatgtag atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa
    24361 atgctttata aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg
    24421 ctgacaacct tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt
    24481 tgtgatcact gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca
    24541 gcgtatggca aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt
    24601 tcactccttc acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt
    24661 tgcgggtttc ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac
    24721 gcacgttggt catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc
    24781 ccgggatgat ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc
    24841 tttcgacatc gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt
    24901 tcagattaag caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg
    24961 ccacggatat atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca
    25021 tttctggctt accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac
    25081 cgttattcca gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag
    25141 taagatgcgg tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg
    25201 tacggtcgcg ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc
    25261 tgctaaggcg atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga
    25321 aaaactcgaa cgaaacatta atctgcccct ggacttctgc caggctctga ttcagggcat
    25381 cccgctggct ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg
    25441 ttgtgttgtg cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa
    25501 gggcgtcaac cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact
    25561 tcaggatggt aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac
    25621 taaccatgta cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc
    25681 tatgataatt caatatattt cattagaaac catagacccg aaaacatgag taccgggcat
    25741 tattaaattt ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc
    25801 cggcggtgac gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc
    25861 agcggctttc gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt
    25921 cacgcaccgc ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc
    25981 gcagcaccag ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt
    26041 catcgccatt ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg
    26101 attccacacg tacctgatcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca
    26161 gcttgcgcat tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct
    26221 gctgccacgc ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca
    26281 gccaggagag cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga
    26341 tcagatcgcg cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc
    26401 agtgacgccc ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg
    26461 ctcccgccag tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact
    26521 gatcagcaag ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg
    26581 cgcgcatcca caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg
    26641 ctaacgcgcg gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca
    26701 cgcctgccag cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga
    26761 acagttccag ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt
    26821 tttctgcgcg gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc
    26881 gcagtactgc caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc
    26941 ctttcagttg ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga
    27001 ccatcgccac cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt
    27061 cgaggatttt cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc
    27121 gatcgttatg cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga
    27181 tctctttatg cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga
    27241 catcatcgtg gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc
    27301 gcgtggaacc ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag
    27361 aaactggctg taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg
    27421 cctcatccag acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac
    27481 tgtatacccc ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga
    27541 tttgtgtcgc ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt
    27601 tacggctgat aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt
    27661 gcagcaacgg gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta
    27721 aaaactcacg ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca
    27781 tcaggcgcgc cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa
    27841 taccactgtc gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc
    27901 tatcggcttc ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg
    27961 tttgttgaac ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca
    28021 actggttagt ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact
    28081 ccgccataat gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa
    28141 atttatgcag ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt
    28201 gtttgcagcc cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc
    28261 ggtggagata cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat
    28321 gcctgcatgt gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc
    28381 cgccagcgta aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc
    28441 cagttcaaaa ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc
    28501 ctgattttcc ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata
    28561 tttcacaatc gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa
    28621 cccgcgtagc agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca
    28681 ccaggcaagt tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc
    28741 agagtgaccg ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag
    28801 cggattttgt aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc
    28861 ccttgccaga tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct
    28921 ttctaccagt accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc
    28981 ttgatcaaca aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata
    29041 gctaccgccg tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt
    29101 cgttaccagg tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc
    29161 cagttctgcc agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc
    29221 atgcgcgcgc ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg
    29281 gacaacgcaa ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg
    29341 tttacgcgtc atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt
    29401 aaacattgtg ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt
    29461 gagcaaatcc caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca
    29521 cgatgtcacg gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa
    29581 attgtggggt ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg
    29641 agcggcgaat atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag
    29701 attacggttt ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt
    29761 caggtgaaca acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat
    29821 gcctttaccg ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa
    29881 atcccggaag cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa
    29941 tactaaagcg gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc
    30001 ctacgtcata ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac
    30061 gccgcatccg gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga
    30121 cgtttaacta tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt
    30181 gcctggcagc cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa
    30241 gcgatccaac tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac
    30301 aaaaagccct acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg
    30361 attgcccatg gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt
    30421 gaacaacagc aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc
    30481 agcttctcat ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag
    30541 aacgcgctgg gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac
    30601 ccgctggtcc aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg
    30661 gtagtttcac cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg
    30721 ccgctgtttt cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct
    30781 tttttcggaa atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc
    30841 aacacggcca ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct
    30901 gcacacgtcg aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca
    30961 agagcagcta gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc
    31021 tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag
    31081 tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg
    31141 tatgcaaact gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag
    31201 tcaggatgat gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt
    31261 tgactatctg ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt
    31321 tggcaaacag caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc
    31381 gctcactgag ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc
    31441 cgtctggcga ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct
    31501 gtttcatgcc ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg
    31561 gtactggttt gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc
    31621 caagcaacaa caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc
    31681 cacgctcgaa tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt
    31741 agcaagtgaa acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg
    31801 gttgcgattg aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac
    31861 tttttctgcc tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag
    31921 tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg
    31981 cctgacaccg caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct
    32041 gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga
    32101 ggcggcgatt gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt
    32161 gattgtcatt gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca
    32221 tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc
    32281 cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg
    32341 cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg
    32401 tcagaaggtg caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca
    32461 ggccaacaat caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg
    32521 ccacgactgc ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca
    32581 ggcgcaacag catttgcgct gtcaccactg tgacagtcag cgtccagtgc cgcgccagtg
    32641 cccttcctgc ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca
    32701 gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag
    32761 ccgcaaaggg gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt
    32821 gatcggcaca caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt
    32881 actggatgtg gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca
    32941 gctttacacc caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct
    33001 gcaaacgcac catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc
    33061 ctttgccgaa caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca
    33121 tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact
    33181 gcgtaatctg atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt
    33241 tccggcactg gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc
    33301 ttcccgcgtg cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc
    33361 ggattcccgc aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta
    33421 cgatgcgagg cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac
    33481 cgttttcacg cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac
    33541 gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc
    33601 atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg
    33661 aatcccgata aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg
    33721 ggttatttac cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg
    33781 gtgattgtcc cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt
    33841 acggcggcaa atcacggata tctggtgctg attggcgact gtgcacatca aaatcagcag
    33901 gaaaaaacct ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt
    33961 tcaaggctgc cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg
    34021 gcgaacgaat ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc
    34081 gcatttgatg cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc
    34141 ggtcccgaag agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt
    34201 cgctgcggaa ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc
    34261 ggaagcaaag caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc
    34321 catagcgatg tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc
    34381 ccggaagacc tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg
    34441 ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg
    34501 cttgatcaaa tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt
    34561 atcatccggg gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg
    34621 gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac
    34681 gatagtggca caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa
    34741 gagcacctca cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat
    34801 tgctgccgcc gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa
    34861 agaagagtcc gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc
    34921 agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc
    34981 cacagaacct tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg
    35041 tcagcttctt gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt
    35101 tccgtggaat gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc
    35161 gcagcaatta gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg
    35221 gcagcagcag acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa
    35281 accagcttct actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc
    35341 gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac
    35401 ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga
    35461 acaggcagag acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac
    35521 caacaatggc tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag
    35581 caccctcaat cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg
    35641 ttgaaaccct caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg
    35701 gtttgtactc tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc
    35761 cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg
    35821 aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg
    35881 ggcggtactg cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat
    35941 cagggccatc tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg
    36001 ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc
    36061 ggtaacggtg acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct
    36121 tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt
    36181 gctgaaaagg cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc
    36241 atcgaagaat taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa
    36301 atcgtcagcg aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg
    36361 attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg
    36421 accccgaaaa atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt
    36481 cgtctggcta agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa
    36541 gtgggctacg tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg
    36601 aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa
    36661 gaacgtattc tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag
    36721 cagcaggaac cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt
    36781 gatgacaaag aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct
    36841 cctccgggca tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc
    36901 cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa
    36961 gaagcggcga aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag
    37021 cagcacggga tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc
    37081 ggtccggatg tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc
    37141 accgtttcca ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc
    37201 gcgttccaga ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc
    37261 cgcgttgaac tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat
    37321 gcctctatca ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt
    37381 accgactccg gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa
    37441 aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac
    37501 gacgccagcg atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat
    37561 ctggatgcgt tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc
    37621 attttcatca ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact
    37681 gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc
    37741 ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat
    37801 ccgctggtcg ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc
    37861 gccaatgatt acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta
    37921 cgcggcatgc aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc
    37981 gtcgtgctca tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc
    38041 gattttgtcg gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc
    38101 gtgggcaatc gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt
    38161 ggctggttga gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg
    38221 atccttccgg cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg
    38281 cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa
    38341 gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg
    38401 tttaatctct tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta
    38461 ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg
    38521 ctggaacgta ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc
    38581 ctaagccagt gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca
    38641 gcccacatag cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat
    38701 acgatacttc cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt
    38761 tctccaactt tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg
    38821 aggacaacgg gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg
    38881 tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag
    38941 tacaaaatga atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg
    39001 aagagttgga tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag
    39061 gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc
    39121 atctttatgc cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat
    39181 aagaaaggca ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa
    39241 attacgcgaa agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga
    39301 cgttcctgcc agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg
    39361 cgctgatgct gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct
    39421 tcgatttcca tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt
    39481 ttctcaaaca cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat
    39541 aaaaaatcct cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag
    39601 ctccgattgt atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt
    39661 gccatacttt tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa
    39721 tttgttgcgc gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg
    39781 cgataacgct cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg
    39841 ctcgttaacg ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg
    39901 catacaacaa acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg
    39961 ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt
    40021 tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg
    40081 actgggggtg gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc
    40141 cgctgttacc attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt
    40201 tatcgtttca caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta
    40261 caatttattt ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt
    40321 tgatctggct ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt
    40381 cgcagttgag atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga
    40441 tggcaacggt gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt
    40501 cattggcgca tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg
    40561 tccgaaagtc tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga
    40621 cattccttac ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt
    40681 tgcctaccgc aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa
    40741 ggaaaccaca actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa
    40801 acatgactga aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt
    40861 cgtaatggat cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta
    40921 cccaaaacca ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac
    40981 gctggtagaa gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat
    41041 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
    41101 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
    41161 tttagaagat tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac
    41221 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
    41281 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
    41341 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
    41401 ggacgacaaa atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
    41461 ttcttccgaa gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
    41521 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
    41581 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
    41641 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
    41701 ctatgcgttg gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga
    41761 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
    41821 caatggtgtg tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta
    41881 tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
    41941 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
    42001 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
    42061 gcagttccag tccgatgttc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac
    42121 cgcattgggt gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga
    42181 gctgcaagag aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg
    42241 taattaccgt tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca
    42301 cgacgagtaa tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct
    42361 tacgcgtctt atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca
    42421 cgccgcatcc ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa
    42481 taatcccacc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
    42541 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
    42601 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
    42661 gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg
    42721 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
    42781 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
    42841 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
    42901 agaagctgat tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg
    42961 ataacctgcg caatgtagca gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga
    43021 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg
    43081 tatttgctat tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
    43141 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga
    43201 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
    43261 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
    43321 tcgaagccgg taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct
    43381 ctgccaccgg tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg
    43441 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca
    43501 tccactatct ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat
    43561 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
    43621 caagtcaatc aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag
    43681 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
    43741 ccgccgggca atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
    43801 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
    43861 atggcaaatt aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta
    43921 gcgaagcagc tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc
    43981 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
    44041 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
    44101 tgccactgat gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg
    44161 tcagtcggga aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg
    44221 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
    44281 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
    44341 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
    44401 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
    44461 tgccggcatc aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg
    44521 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
    44581 tgtcttcggc cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt
    44641 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
    44701 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
    44761 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
    44821 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
    44881 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
    44941 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
    45001 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
    45061 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
    45121 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
    45181 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
    45241 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
    45301 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
    45361 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
    45421 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
    45481 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt
    45541 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
    45601 atcatgcaag cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa
    45661 cgccgcggtc tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc
    45721 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
    45781 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
    45841 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
    45901 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
    45961 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
    46021 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag
    46081 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
    46141 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
    46201 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
    46261 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
    46321 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
    46381 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
    46441 gaccttcgct gttgaaccaa ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
    46501 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
    46561 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
    46621 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
    46681 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
    46741 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
    46801 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
    46861 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
    46921 gagtctgacg aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg
    46981 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc
    47041 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
    47101 atcgcttacg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
    47161 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
    47221 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
    47281 cagccagaca tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
    47341 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
    47401 atatccggca cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc
    47461 ctggatttcc tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa
    47521 cacaaacgaa ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
    47581 cgcctcaggc acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc
    47641 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
    47701 gctgctgatg ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga
    47761 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
    47821 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccagac
    47881 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
    47941 acgatacgtt ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
    48001 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
    48061 caaacattcc tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
    48121 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
    48181 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
    48241 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc
    48301 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
    48361 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
    48421 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
    48481 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
    48541 ccactttatc gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga
    48601 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga
    48661 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
    48721 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
    48781 tgttgttgtg atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag
    48841 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
    48901 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
    48961 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
    49021 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
    49081 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
    49141 gtttccagtg ggagctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
    49201 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
    49261 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
    49321 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
    49381 gtatatgttg agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
    49441 ctttgtccat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc
    49501 aataaattgc agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct
    49561 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
    49621 gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
    49681 tcgtggtgaa ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc
    49741 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
    49801 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
    49861 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
    49921 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
    49981 tcaacttccg gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga
    50041 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
    50101 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
    50161 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc
    50221 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
    50281 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
    50341 tccgggaaac gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg
    50401 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc
    50461 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
    50521 cctggcgcat caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa
    50581 ctttggaatg caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
    50641 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
    50701 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
    50761 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat
    50821 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
    50881 aaaggcagag agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc
    50941 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
    51001 tcttgccgtt cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    51061 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    51121 cgatgccagc cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca
    51181 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    51241 caccgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    51301 ccgacgcctg gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac
    51361 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    51421 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt
    51481 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    51541 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc
    51601 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    51661 gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc
    51721 aaattgagga taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc
    51781 aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat
    51841 gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg
    51901 tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc
    51961 atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg
    52021 ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta
    52081 acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt
    52141 tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca
    52201 cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc
    52261 agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct
    52321 gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta
    52381 aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat
    52441 ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa
    52501 ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct
    52561 tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa
    52621 tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac
    52681 ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct
    52741 cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc
    52801 gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc
    52861 cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac
    52921 cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg
    52981 tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga
    53041 tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt
    53101 taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc
    53161 gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg
    53221 ttacccaagc tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg
    53281 ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg
    53341 tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta
    53401 ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt
    53461 aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg
    53521 gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt
    53581 cgtccggcca gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta
    53641 ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg
    53701 gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc
    53761 cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac
    53821 cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc
    53881 tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt
    53941 cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc
    54001 atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa
    54061 accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag
    54121 caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat
    54181 ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat
    54241 acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac
    54301 gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc
    54361 gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc
    54421 gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg
    54481 ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa
    54541 ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt
    54601 atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt
    54661 gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga
    54721 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    54781 ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt
    54841 ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc
    54901 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac
    54961 ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag
    55021 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg
    55081 aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg
    55141 cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat
    55201 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    55261 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    55321 ccccattaga ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc
    55381 aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtggg
//
LOCUS       DAEEGA010000018        54200 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEEGA010000018 DAEEGA010000000
VERSION     DAEEGA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54200
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.018"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            134..433
                     /locus_tag="KLH46_001122"
     CDS             134..433
                     /locus_tag="KLH46_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0377889.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            443..967
                     /gene="ygaP"
                     /locus_tag="KLH46_001123"
     CDS             443..967
                     /gene="ygaP"
                     /locus_tag="KLH46_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBE0377890.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1014..1418)
                     /gene="stpA"
                     /locus_tag="KLH46_001124"
     CDS             complement(1014..1418)
                     /gene="stpA"
                     /locus_tag="KLH46_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBE0377891.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2086..2535
                     /gene="alaE"
                     /locus_tag="KLH46_001125"
     CDS             2086..2535
                     /gene="alaE"
                     /locus_tag="KLH46_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBE0377892.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2572..2916)
                     /locus_tag="KLH46_001126"
     CDS             complement(2572..2916)
                     /locus_tag="KLH46_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBE0377893.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3068..3298
                     /locus_tag="KLH46_001127"
     CDS             3068..3298
                     /locus_tag="KLH46_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0377894.1"
                     /translation="MYNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3645..3890
                     /gene="nrdH"
                     /locus_tag="KLH46_001128"
     CDS             3645..3890
                     /gene="nrdH"
                     /locus_tag="KLH46_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBE0377895.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3887..4297
                     /gene="nrdI"
                     /locus_tag="KLH46_001129"
     CDS             3887..4297
                     /gene="nrdI"
                     /locus_tag="KLH46_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBE0377896.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4270..6414
                     /gene="nrdE"
                     /locus_tag="KLH46_001130"
     CDS             4270..6414
                     /gene="nrdE"
                     /locus_tag="KLH46_001130"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBE0377897.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRASPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6424..7383
                     /gene="nrdF"
                     /locus_tag="KLH46_001131"
     CDS             6424..7383
                     /gene="nrdF"
                     /locus_tag="KLH46_001131"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBE0377898.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7738..8940
                     /gene="proV"
                     /locus_tag="KLH46_001132"
     CDS             7738..8940
                     /gene="proV"
                     /locus_tag="KLH46_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBE0377899.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8933..9997
                     /gene="proW"
                     /locus_tag="KLH46_001133"
     CDS             8933..9997
                     /gene="proW"
                     /locus_tag="KLH46_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBE0377900.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10055..11047
                     /gene="proX"
                     /locus_tag="KLH46_001134"
     CDS             10055..11047
                     /gene="proX"
                     /locus_tag="KLH46_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBE0377901.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11239..12418
                     /locus_tag="KLH46_001135"
                     /pseudo
     CDS             11239..12418
                     /locus_tag="KLH46_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12542..13279
                     /gene="ygaZ"
                     /locus_tag="KLH46_001136"
     CDS             12542..13279
                     /gene="ygaZ"
                     /locus_tag="KLH46_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBE0377902.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13269..13604
                     /gene="ygaH"
                     /locus_tag="KLH46_001137"
     CDS             13269..13604
                     /gene="ygaH"
                     /locus_tag="KLH46_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBE0377903.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13695..14225
                     /gene="emrR"
                     /locus_tag="KLH46_001138"
     CDS             13695..14225
                     /gene="emrR"
                     /locus_tag="KLH46_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBE0377904.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14352..15524
                     /gene="emrA"
                     /locus_tag="KLH46_001139"
                     /pseudo
     CDS             14352..15524
                     /gene="emrA"
                     /locus_tag="KLH46_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15541..17079
                     /gene="emrB"
                     /locus_tag="KLH46_001140"
     CDS             15541..17079
                     /gene="emrB"
                     /locus_tag="KLH46_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBE0377905.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFDAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17143..17658)
                     /gene="luxS"
                     /locus_tag="KLH46_001141"
     CDS             complement(17143..17658)
                     /gene="luxS"
                     /locus_tag="KLH46_001141"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBE0377906.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17808..19364)
                     /gene="gshA"
                     /locus_tag="KLH46_001142"
     CDS             complement(17808..19364)
                     /gene="gshA"
                     /locus_tag="KLH46_001142"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBE0377907.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19437..19865)
                     /locus_tag="KLH46_001143"
     CDS             complement(19437..19865)
                     /locus_tag="KLH46_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0377908.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19862..20428)
                     /gene="yqaB"
                     /locus_tag="KLH46_001144"
     CDS             complement(19862..20428)
                     /gene="yqaB"
                     /locus_tag="KLH46_001144"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBE0377909.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(20576..20652)
                     /locus_tag="KLH46_001145"
     tRNA            complement(20576..20652)
                     /locus_tag="KLH46_001145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20616..20618),aa:Arg,seq:acg)
     gene            complement(20717..20793)
                     /locus_tag="KLH46_001146"
     tRNA            complement(20717..20793)
                     /locus_tag="KLH46_001146"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20757..20759),aa:Arg,seq:acg)
     gene            complement(20858..20934)
                     /locus_tag="KLH46_001147"
     tRNA            complement(20858..20934)
                     /locus_tag="KLH46_001147"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20898..20900),aa:Arg,seq:acg)
     gene            complement(20999..21075)
                     /locus_tag="KLH46_001148"
     tRNA            complement(20999..21075)
                     /locus_tag="KLH46_001148"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21039..21041),aa:Arg,seq:acg)
     gene            complement(21079..21171)
                     /locus_tag="KLH46_001149"
     tRNA            complement(21079..21171)
                     /locus_tag="KLH46_001149"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21135..21137),aa:Ser,seq:gct)
     gene            complement(21487..21672)
                     /gene="csrA"
                     /locus_tag="KLH46_001150"
     CDS             complement(21487..21672)
                     /gene="csrA"
                     /locus_tag="KLH46_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBE0377910.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(21907..24537)
                     /gene="alaS"
                     /locus_tag="KLH46_001151"
     CDS             complement(21907..24537)
                     /gene="alaS"
                     /locus_tag="KLH46_001151"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBE0377911.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(24665..25165)
                     /gene="recX"
                     /locus_tag="KLH46_001152"
     CDS             complement(24665..25165)
                     /gene="recX"
                     /locus_tag="KLH46_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBE0377912.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(25408..26469)
                     /gene="recA"
                     /locus_tag="KLH46_001153"
     CDS             complement(25408..26469)
                     /gene="recA"
                     /locus_tag="KLH46_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBE0377913.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(26549..27046)
                     /gene="pncC"
                     /locus_tag="KLH46_001154"
     CDS             complement(26549..27046)
                     /gene="pncC"
                     /locus_tag="KLH46_001154"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBE0377914.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(27191..28276)
                     /gene="mltB"
                     /locus_tag="KLH46_001155"
     CDS             complement(27191..28276)
                     /gene="mltB"
                     /locus_tag="KLH46_001155"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBE0377915.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            28532..29094
                     /gene="srlA"
                     /locus_tag="KLH46_001156"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             28532..29094
                     /gene="srlA"
                     /locus_tag="KLH46_001156"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            29091..30050
                     /gene="srlE"
                     /locus_tag="KLH46_001157"
                     /gene_synonym="gutE"
     CDS             29091..30050
                     /gene="srlE"
                     /locus_tag="KLH46_001157"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBE0377916.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSSFIYQ"
     gene            30061..30432
                     /gene="srlB"
                     /locus_tag="KLH46_001158"
                     /gene_synonym="gutB"
     CDS             30061..30432
                     /gene="srlB"
                     /locus_tag="KLH46_001158"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBE0377917.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            30436..31215
                     /gene="srlD"
                     /locus_tag="KLH46_001159"
     CDS             30436..31215
                     /gene="srlD"
                     /locus_tag="KLH46_001159"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBE0377918.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            31320..31679
                     /gene="gutM"
                     /locus_tag="KLH46_001160"
                     /pseudo
     CDS             31320..31679
                     /gene="gutM"
                     /locus_tag="KLH46_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            31746..32519
                     /gene="srlR"
                     /locus_tag="KLH46_001161"
                     /gene_synonym="gutR"
     CDS             31746..32519
                     /gene="srlR"
                     /locus_tag="KLH46_001161"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBE0377919.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            32512..33477
                     /gene="gutQ"
                     /locus_tag="KLH46_001162"
     CDS             32512..33477
                     /gene="gutQ"
                     /locus_tag="KLH46_001162"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBE0377920.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(33474..34988)
                     /gene="norR"
                     /locus_tag="KLH46_001163"
     CDS             complement(33474..34988)
                     /gene="norR"
                     /locus_tag="KLH46_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBE0377921.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            35175..36614
                     /gene="norV"
                     /locus_tag="KLH46_001164"
     CDS             35175..36614
                     /gene="norV"
                     /locus_tag="KLH46_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBE0377922.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            36611..37744
                     /gene="norW"
                     /locus_tag="KLH46_001165"
     CDS             36611..37744
                     /gene="norW"
                     /locus_tag="KLH46_001165"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBE0377923.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(37872..40124)
                     /gene="hypF"
                     /locus_tag="KLH46_001166"
     CDS             complement(37872..40124)
                     /gene="hypF"
                     /locus_tag="KLH46_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBE0377924.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(40277..40804)
                     /gene="hydN"
                     /locus_tag="KLH46_001167"
     CDS             complement(40277..40804)
                     /gene="hydN"
                     /locus_tag="KLH46_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBE0377925.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(40953..41963)
                     /gene="ascG"
                     /locus_tag="KLH46_001168"
     CDS             complement(40953..41963)
                     /gene="ascG"
                     /locus_tag="KLH46_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBE0377926.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(41977..42096)
                     /locus_tag="KLH46_001169"
     CDS             complement(41977..42096)
                     /locus_tag="KLH46_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HBE0377927.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(42106..43815)
                     /gene="hycE"
                     /locus_tag="KLH46_001170"
     CDS             complement(42106..43815)
                     /gene="hycE"
                     /locus_tag="KLH46_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBE0377928.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(43833..44756)
                     /gene="hycD"
                     /locus_tag="KLH46_001171"
     CDS             complement(43833..44756)
                     /gene="hycD"
                     /locus_tag="KLH46_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBE0377929.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(44759..46552)
                     /gene="hycC"
                     /locus_tag="KLH46_001172"
     CDS             complement(44759..46552)
                     /gene="hycC"
                     /locus_tag="KLH46_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBE0377930.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGS
                     VWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSN
                     SGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVS
                     VVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMA
                     ICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVP
                     GWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(46589..47200)
                     /gene="hycB"
                     /locus_tag="KLH46_001173"
     CDS             complement(46589..47200)
                     /gene="hycB"
                     /locus_tag="KLH46_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBE0377931.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHRCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(47325..47786)
                     /gene="hycA"
                     /locus_tag="KLH46_001174"
     CDS             complement(47325..47786)
                     /gene="hycA"
                     /locus_tag="KLH46_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBE0377932.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLYH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            47998..48348
                     /gene="hypA"
                     /locus_tag="KLH46_001175"
     CDS             47998..48348
                     /gene="hypA"
                     /locus_tag="KLH46_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBE0377933.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            48352..49224
                     /gene="hypB"
                     /locus_tag="KLH46_001176"
     CDS             48352..49224
                     /gene="hypB"
                     /locus_tag="KLH46_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBE0377934.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            49215..49487
                     /gene="hypC"
                     /locus_tag="KLH46_001177"
     CDS             49215..49487
                     /gene="hypC"
                     /locus_tag="KLH46_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBE0377935.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            49487..50608
                     /gene="hypD"
                     /locus_tag="KLH46_001178"
     CDS             49487..50608
                     /gene="hypD"
                     /locus_tag="KLH46_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBE0377936.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            50605..51615
                     /gene="hypE"
                     /locus_tag="KLH46_001179"
     CDS             50605..51615
                     /gene="hypE"
                     /locus_tag="KLH46_001179"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBE0377937.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            51689..53767
                     /gene="flhA"
                     /locus_tag="KLH46_001180"
     CDS             51689..53767
                     /gene="flhA"
                     /locus_tag="KLH46_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBE0377938.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(53804..54157)
                     /locus_tag="KLH46_001181"
     CDS             complement(53804..54157)
                     /locus_tag="KLH46_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBE0377939.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT        12891 a        14615 c        13887 g        12807 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctattcag cttacgcttt tactctcatt
       61 tacgctgctg aggctggtat atgttgcatt tttagtttag agtttactta atttagaaaa
      121 tacttaaata aatatgactg aactcgcgca attacaggcc agtaccgaac aggcagcggc
      181 cttattgaaa gcaatgagcc accctaaacg gttgctgatt ctgtgcatgc ttagcggttc
      241 ccccggcacc agcgcgggag agctgacgcg cattaccgga ctgagcgcct ctgcgacatc
      301 acagcatctc gctcgtatgc gggacgaagg gcttatcaac agccaacggg atgcccaacg
      361 cattctatat tccattaaaa atgaggcggt aaatgccatt atcgccaccc tgaaaaatgt
      421 ctattgtccg taaggagtca ctatggcttt gacaaccatt tcgccgcatg atgcacaaga
      481 attaatcgca cgcggcgcaa agttaatcga tattcgtgac gctgatgaat atcttcgtga
      541 acatattcct gaagcagatc tggctccatt atccgtgctg gaacagtcag gtcttccacc
      601 taaattacgt cgcgagcaaa ttatattcca ctgccaggca ggtaaacgca ccagtaataa
      661 cgccgataaa ttagcagcga ttgccgcccc cgcagaaata tttttactcg aagatgggat
      721 tgatggctgg aaaagagctg gattgccagt agcggtaaat aaatctcaac ccttgccgtt
      781 aatgcgccag gtgcagatcg ctgcgggtgg tttaatatta atcggcgttg tactgggtta
      841 taccgtaaat agcggtttct tcttattaag tggctttgtt ggtgccgggt tactgtttgc
      901 aggaatcagc ggtttttgtg gaatggcaag gttgttagat aagatgccgt ggaaccaacg
      961 agcttgagaa gcgacgccgg acgcgcccta gcagcgacat ccggcctcag taattagatc
     1021 aggaaatcgt cgagagattt accttctgcc agcgcctgag caattggctt cggtgtacgg
     1081 ccctgaccgg tccaggtttt agtttcaccg ttaacatcag tgaatttata tttcgccgga
     1141 cgcggctggc gttttttacc agcacgtggt gcagcagcag agctattacc caataactct
     1201 tccgggttaa ttccgtcagc tttcatcagc tccagccagg tgctaatttt ttcctgacgc
     1261 tcagccagtt cacgctgctg ctgttcttct tcttcacgtc tttctttagt gacaaccctg
     1321 aatttttcga gcatttcttc aagaacgtca atggagaatt cgcgagccat cgcacggagg
     1381 gtgcgaatgt tatttaaact ttgtaacatt acggacataa taaagaaaac cttttaacgc
     1441 caaaacaaaa aagtatttcg tcgcgtagtg taagcgatca cttattatat ttccacaaaa
     1501 tttaatctat ttacagtcct gcgcgagatt atttcagctg ttattttctg aaactctcag
     1561 aataatttca agattatata ttagccattt tgatgagtaa tttaggttat tggtgaacag
     1621 agtggcgctc tctgctcgct aactccgcaa gcctgacaaa accgaagtga ataaaaatgc
     1681 aatataacag aataaaagaa aacacccctc actggtgctt tctgctgtta atgtacattt
     1741 tctggcatca aaaatgctgt aaatgacccg gtcacgacaa acaccgaatt ctctacgctt
     1801 atagcgttac ctccccccca aactttcatt agtgaaatta aaggcaagat catatctaat
     1861 tgatttaatt gaaaataaat catatcggca tgtaaaaatc cacaatgtac aaaaaacgag
     1921 ccgttacgga atattttatc tacaaaaact aactaaataa aaatttttca ctaattgatt
     1981 agtcatagac agcgatatac gctatgcaaa aatgcagatg gcaatgagat ccactgcttt
     2041 catctccatt aacatcccat tacgctttta ttaaggagca ttagcatgtt ctcaccgcag
     2101 tcacgcttgc gtcatgcagt tgcagatacg ttcgcgatgg ttgtttactg ttctgtcgtg
     2161 aacatgtgta ttgaagtttt cctctccgga atgagcttcg aacagtcttt ttattccaga
     2221 ttggtagcga ttccggtgaa catcttaatt gcatggccat acggtatgta ccgcgatctg
     2281 tttatgcgcg cggcacgcaa agttagcccg tcgggctgga taaaaaatct ggctgatatc
     2341 ctggcttatg tgacgttcca gtcaccggtg tatgtggcga tcttgttagt ggtgggcgca
     2401 gactggcatc agattatggc ggcggtcagt tcaaacatcg ttgtttcgat gttgatgggg
     2461 gcggtttatg gctacttcct cgattattgc cgccgactgt ttaaagtcag ccgttaccag
     2521 caggtaaaag cctgactctt cttttcgcga ctggcatcgc cagtcgcgtc tttaactggc
     2581 ttcgccaaat aaaccattca aataacgttc aagcgcaaca cgcgaactaa agccatgcgg
     2641 gataccgtaa tgctcgtgtc gctcgcctgc taaatagagc ggaaattgct ggtaatgatc
     2701 gcaagtttta atatcggaag cgggttcagc cagtgttgaa ctgcgttctt ttaatgttgg
     2761 atgaattacc agagcggtac gtcccattcg cgcttcgcga ttaacataga cataattttc
     2821 cccacggcgg taaccatacg ctttctgggt taccacatcg acagtaaaac cgactttttc
     2881 aagtacgcgc gccacctcgt ctggtcgtaa atacatattt gatcctcgtt attttcaacc
     2941 gcgggcctac cttacctgat tgcgcattca caacgctttc agaaaagtcc ataaagccgc
     3001 gatgaacctc agttaagaaa attatggtct acactgaaaa ttacatcgaa ttctaatggg
     3061 ggatcatatg tataaccgac cgaaccgcaa cgacgttgat gatggcgtgc aggatatcca
     3121 gaatgatgtc aatcaattag ctgacagcct cgaatctgta ttgaagtcct ggggcagcga
     3181 cgccaaaggg gaagctgaag ccgcacgcag taaagctcag gcgttgctga aagaaaccag
     3241 ggcgcgaatg catggtcgta ctcgcgtcca gcaagccgcg cgcgatgccg ttggctgagc
     3301 ggattctttt gttcgtgaaa gaccctggtg tagcgtgggt acagcagctg cggtaggtat
     3361 ttttatcggc gcactgttaa gcgtgcgcaa atcgtagtgc aaaaatgata ataaatacgc
     3421 gtctttgacc ccgaagcctg tcttcggggt ttctttttgc ctggtgaatc acaaaaatcc
     3481 ccctcccccg tcacgctcat atccagggta atttcgatca ctatttgcta tatattgtgt
     3541 ggttgaatct tttttcaact acatctagta tctctgtatc aacagagaga caacccgacg
     3601 cgtatcatcg cgccgtatct tcattttaaa cggaaatacg aatcatgcgc ataactattt
     3661 atactcgtaa cgattgcgtt cagtgccacg ccaccaaacg ggcgatggaa aaccggggct
     3721 ttgattttga aatgattaat gtcgatcgcg ttcctgaagc agcggaagcg ttgcgtgctc
     3781 agggctttcg tcagttgccg gtagtgattg ctggcgatct tagctggtct ggtttccgtc
     3841 cggacatgat taaccgtctg catccagcgc cacacgcggc cagtgcatga gccagctcgt
     3901 ctacttctcc agcagctccg aaaacacgca gcgttttatc gaacgtttag gtctgcccgc
     3961 ggtgcgcatc ccgctcaatg agcgggaacg gattcaggta gacgagcctt acatcctgat
     4021 cgtgccctct tacggcggcg gcggtacggc tggcgcggtg ccacgacagg taattcgctt
     4081 tttaaacgac gagcacaacc gggcgttgct tcgcggcgtt attgcttctg gtaatcgcaa
     4141 ctttggtgag gcgtatggcc gcgccggaga tgtgattgcc cggaaatgcg gcgtgccgtg
     4201 gctgtaccgt tttgaactca tgggtacgca aagcgatatc gaaaacgttc gtaaaggagt
     4261 aaccgaattt tggcaacgac aaccgcagaa tgcctgacgc aggaaacgat ggattaccac
     4321 gcgctgaatg cgatgcttaa tctttacgat agcgcaggtc gcattcagtt cgataaagac
     4381 cgccaggccg ttgacgcctt tattgcgacg catgtgcgtc cgaacagtgt gaccttcagt
     4441 agccagcagc agcgcctgaa ctggctggtc aacgaaggtt actatgatga aagcgttctt
     4501 aatcgctact ctcgcgactt tgtcattacg ctgtttgccc acgcacacac cagcggtttt
     4561 cgtttccaga cattcctcgg ggcatggaag ttttacacca gctatacgtt gaagacattc
     4621 gacggtaaac gttatctgga agattttgcc gatcgagtca cgatggtggc gctgacgctg
     4681 gcacaaggcg atgagacgct ggcgttgcaa ctgaccgatg aaatgctgtc aggacgcttt
     4741 cagccagcca cgccaacatt cctcaactgc ggtaagcagc agcgcggcga actggtttcc
     4801 tgttttttgc tgcgtattga agacaatatg gagtcgattg gtcgggcggt aaattccgca
     4861 ctgcagctgt cgaaacgcgg cggcggcgta gcatttttgc tgtcgaatct gcgggaagcg
     4921 ggcgcgccaa ttaaacgtat tgaaaatcaa tcttctggcg taattccggt gatgaaaatg
     4981 ttggaagacg cattttccta tgccaaccaa ctcggcgcgc gtcagggggc aggtgcagtc
     5041 tatttacatg ctcatcatcc cgatattctg cgttttctcg acacgaaacg ggaaaatgcc
     5101 gacgaaaaaa tccgcattaa aacactgtcg ctgggcgtgg tgatcccgga tatcactttc
     5161 catctggcaa aagagaatgc gcagatggcg ctgttttcgc cctatgacgt tgagcgagtt
     5221 tatggcaagc cgtttgccga tatcgccatt agcgaacact atgacgaact ggttgccgat
     5281 gaacgcatcc gcaaaaaata cctcaacgcc cgtgatttct tccagcgact ggcagaaatc
     5341 cagtttgagt ccggctatcc ctacatcatg tatgaagaca cagtaaaccg tgctagccct
     5401 atcgccgggc gcataaatat gagtaatctc tgctcagaaa ttttgcaggt taacagcgcc
     5461 tcagagtatg acgagaatct cgactatgcc cgcacaggcc atgatatttc ctgcaattta
     5521 ggttcgttga atattgcgca caccatggat tcccccgatt tttcccgcac ggtagagact
     5581 gccgtgcgcg gtttaacggc ggtgtcagat atgagtcata tccgcagcgt gccgtccatc
     5641 gaagccggaa atgccgcctc gcacgccatc ggactggggc agatgaattt acatggttat
     5701 ctggcgaggg aaggcatcgc ttatggttcg ccggaagccc tggatttcac caatctctat
     5761 ttctatacca tcacctggca cgcactgcgt acctcgatgt tgctggcgcg cgaacgcggt
     5821 gaaaccttcg ccgggttcaa acagtcacgc tatgccagtg gtgaatattt cagccaatat
     5881 ctgcaaggta actggcagcc gaaaacggcg aaagttggcg aactgtttgc ccgcagctgt
     5941 attacgttac ctacccgtga gatgtgggcg caactgcgcg acgacgtgat gcgctacggc
     6001 atatacaacc agaatctcca ggcggtgccg ccaaccgggt ctatctctta tatcaaccat
     6061 gctacgtcga gtattcatcc gattgtggcg aaagtagaga tacgcaaaga gggcaaaaca
     6121 ggacgcgttt actaccctgc cccgtttatg actaacgaaa atctggcgct gtatcaggac
     6181 gcttacgaaa ttggcgcaga aaagatcatc gacacctacg cggaagcgac tcgccatgtc
     6241 gatcaggggc tgtcgctgac gctgtttttc cccgataccg ccaccactcg cgatatcaac
     6301 aaagcgcaga tttacgcctg gcgcaaaggt atcaaaacgc tctattacat ccgcctgcgt
     6361 cagatggcgc tggaaggcac tgaaattgaa ggctgcgtct cctgtgcact ttaaggaata
     6421 tctatgaaac tctcacgtat cagcgccatc aactggaaca agatatctga cgataaagat
     6481 ctggaggtgt ggaatcgcct gaccagcaat ttctggctac cagaaaaggt gccgctgtcg
     6541 aacgatattc ccgcctggca gacattaact gtcgtagaac aacaactgac gatgcgcgtt
     6601 tttactggcc tgacgctgct cgacacgctg caaaatgtta tcggcgcgcc ttcgctgatg
     6661 cccgatgcgc tcacgcctca tgaagaggcg gtattatcga atatcagctt tatggaagcg
     6721 gttcatgccc gctcttacag ttcgattttc tcgacgctgt gccagaccaa agatgtcgat
     6781 gccgcctacg cctggagtga agaaaacgca ccgttgcagc gaaaagctca gattattcag
     6841 caacattatc gcggtgatga tccgctgaaa aagaaaatcg ccagtgtgtt tcttgaatct
     6901 tttttgttct attccggttt ctggctgccg atgtattttt ccagccgcgg aaagctgacc
     6961 aataccgcgg acctgatccg tctgattatc cgcgatgaag cagtccacgg ttactacata
     7021 ggctataaat atcagaaaaa catggaaaag atatctctgg gacaacgtga agagttgaag
     7081 agtttcgcct tcgatttgtt gctggaactc tacgacaacg agttgcaata caccgatgag
     7141 ctgtacgccg aaaccccgtg ggctgacgat gtgaaagcgt ttctctgtta caacaccaat
     7201 aaggctttga tgaatctggg ctacgaaccg ttatttcccg cagaaatggc ggaagtgaat
     7261 ccggcaatcc tcgccgcgct ttcgccgaat gccgatgaaa atcacgattt cttttccggt
     7321 tcaggctcct cttatgtgat ggggaaagcg gttgaaacag aagatgaaga ctggaatttc
     7381 tgagggtgtt attttcaaaa atatcactac ccgcagcagg gaaataattc ccgccaaata
     7441 gctttttatc acgcaaatat tctgtagtga tctacactga tactctgttg cattattcgc
     7501 ctgaaactac aatattcagg cgttttttcg ctatctatga caaaaaatat caacttttct
     7561 cgatttgctc tcagccctta tatcacggaa aattccggcg atttgctcgc atcaatattc
     7621 atgccacatt tgccctcagg ggttgcctca gattctcagt atgttagggt agaaaaaagt
     7681 gactattccc attgggtaat atatcgacat aaacaaataa aggaatcatt ctattgcatg
     7741 gccattaaat taaaaattaa aaatctttat aaaatatttg gtgagcatcc acagcgagcg
     7801 ttcaaatata tcgaacaagg actttcaaaa gaacaaattc tggaaaaaac tgggctatcg
     7861 cttggcgtaa aagacgccag tctggccatt gaagaaggcg agatatttgt catcatggga
     7921 ttatccggct cgggtaaatc cacaatggta cgccttctca atcgcctgat tgaacccacc
     7981 cgcgggcaag tgctgattga tggtgtggat attgccaaaa tatccgacgc cgaactccgt
     8041 gaggtgcgca gaaaaaagat tgcgatggtc ttccagtcct ttgccttaat gccgcatatg
     8101 accgtgctgg acaatactgc gttcggtatg gaattggccg gaattaatgc cgaagaacgc
     8161 cgggaaaaag cccttgatgc actgcgtcag gtcgggctgg aaaattatgc ccacagctac
     8221 ccggatgaac tctctggcgg gatgcgtcaa cgtgtgggat tagcccgcgc gttagcgatt
     8281 aatccggata tattattaat ggacgaagcc ttctcggcgc tcgatccatt aattcgcacc
     8341 gagatgcagg atgagctggt aaaattacag gcgaaacatc agcgcaccat tgtctttatt
     8401 tcccacgatc ttgatgaagc catgcgtatt ggcgaccgaa ttgccattat gcaaaatggt
     8461 gaagtggtac aggtcggcac accggatgaa attctcaata atccggcgaa tgattatgtc
     8521 cgtaccttct tccgtggcgt tgatattagt caggtgttca gtgcgaaaga tattgcccgc
     8581 cggacaccga atggcttaat tcgtaaaact cctggcttcg gcccacgttc ggcactgaaa
     8641 ttattgcagg atgaagatcg cgaatatggc tacgttatcg aacgcggtaa taagtttgtc
     8701 ggcgcagtct ccatcgattc gcttaaaacc gcgttaacgc agcagcaagg tcttgatgcg
     8761 gcgctgattg atgcgccgtt agcagtcgat gcacaaacgc ctcttagcga gttgctctct
     8821 catgtcggac aggcaccctg tgcggtgccc gtggtcgacg aggaccaaca gtatgtcggc
     8881 atcatttcga aaggaatgct gctgcgcgct ttagatcgtg agggggtaaa taatggctga
     8941 tcaaaataat ccgtgggata ccacgccagc ggcggacagt gccgcgcaat ccgcagacgc
     9001 ctggggtaca ccgacgactg caccggctga cggcggtggt gctgactggc tgaccagtac
     9061 gcctgcgcca aacgtcgagc attttaatat tctcgatccg ttccataaaa cgctgatccc
     9121 gctcgacagt tgggtcactg aagggatcga ctgggttgtt acccatttcc gtcccgtctt
     9181 ccagggcgtg cgcgttccgg ttgattatat cctcaacggt ttccagcaat tgctgctggg
     9241 tatgccagcg ccagtggcga ttatcgtttt cgctctcatc acctggcaga tttccggggt
     9301 cggaatgggc gtggcgacgc tggtctcgct gattgccatc ggcgcaatcg gtgcctggtc
     9361 gcaggcaatg gtgactctgg cgctggtgtt aactgccctg ctgttctgta tcgtcatcgg
     9421 tttgccgttg gggatatggc tggcgagaag tccgcgagcg gcgaaaatta ttcgtccact
     9481 gcttgatgcc atgcagacca cgccagcgtt tgtttatctg gtgccaatcg tcatgctatt
     9541 tggtatcggt aacgtgccgg gcgtggtggt gacgatcatc tttgcgctgc cgccgattat
     9601 ccgtctgacc attctgggga ttaaccaggt tccggcggat ctgattgaag cctcgcgctc
     9661 attcggtgcc agcccgcgcc agatgctgtt caaagttcag ttaccgctgg cgatgccgac
     9721 cattatggcg ggcgttaacc agacgctgat gctggccctt tctatggtgg tcattgcctc
     9781 gatgattgcc gtcggcgggc tgggtcagat ggtacttcgc ggtatcggtc gtctggatat
     9841 ggggcttgcc accgttggcg gcgtcgggat tgtgatcctc gccattatcc tcgatcgcct
     9901 gacgcaggcc gttgggcgcg actcacgcag tcgcggcaac cgtcgctggt acaccactgg
     9961 ccctgttggt ctgctgaccc gcccattcat taagtaactc tgcacttgcc cggtggcgcc
    10021 gggcattatc accctgccaa aaaaaggaat aacaatgcga catagcgtac tttttgcgac
    10081 agcgtttgcc acgcttatct ctacacaaac ttttgctgcc gatctgccgg gcaaaggcat
    10141 tactgttaat ccagttcaga gcaccatcac tgaagaaacc ttccagacgc tgctggtcag
    10201 tcgtgcgctg gagaaattag gttataccgt caacaaaccc agcgaagtgg attacaacgt
    10261 tggctacacc tcgcttgctt ccggcgatgc aaccttcacc gccgtgaact ggacgccact
    10321 ccatgacaac atgtacgaag ctgccggtgg cgataagaaa ttttatcgtg aaggggtatt
    10381 tgttaacggc gcagcacagg gttacctgat cgataagaaa accgccgacc aatacaaaat
    10441 caccaacatc gcacaactga aagatccgaa gatcgccaaa ctgttcgata ccaacggcga
    10501 cggtaaagcg gatttaaccg gttgtaaccc tggctggggc tgcgaaggtg cgatcaacca
    10561 ccagcttgcc gcgtatgaac tgaccaacac cgtgactcat aatcagggga actacgcagc
    10621 gatgatggcc gacaccatca gtcgctacaa agagggcaaa ccggtgtttt attacacctg
    10681 gacgccgtac tgggtgagta atgagctgaa gccagggaaa gatgtggtct ggttgcaggt
    10741 gccgttctcc gcactgccgg gcgataaaaa tgccgatacc aaactgccga atggcgcgaa
    10801 ttacggcttc ccggtcagca ccatgcatat cgttgccaac aaagcctgga ccgagaaaaa
    10861 tccggcggca gccaaactgt ttgccattat gcagttgcca gtggccgata ttaacgccca
    10921 gaacgccatt atgcatgacg gcaaagcctc agaaggcgat attcagggcc acgttgatgg
    10981 ctggatcaaa gcccaccagc agcagttcga tggctgggtg aatgaggcgc tggcagcgca
    11041 gaagtaattt ttattcgggc ggataaggcg ttttcgcctc atccgccgct ctgtacacaa
    11101 tgcctgatgc gacgctgacg cgtcctatca tgcctacaaa ccacatcgca catttcaaca
    11161 agctatcttt catcccatat tcatcaacat ccgctattat tgatttccag cttaatcatc
    11221 acctgatgaa caaaaataat gactaaacct aatcatgagc ttagcccggc gctgatcgtg
    11281 ctgatgtcta tcgccaccgg tctggcggta gccagtaact attacgccca gccattgctc
    11341 gacaccatcg cgcgtaactt ttccctttcc gccagttcgg caggctttat tgttaccgcc
    11401 gcgcagttgg gctatgccgc aggtctactg tttcttgttc ccctcggtga tatgtttgaa
    11461 cgccgccgcc tgattgtctc gatgacctta ctggcagcgg gcggtatgtt aatcactgcc
    11521 agcagtcagt cactggcgat gatgatcctc ggtacggcat taaccggttt attctcagtc
    11581 gtggcacaaa ttctggttcc gctggcagcg acgctggctt caccggataa acgcggcaaa
    11641 gtggttggca ccattatgag cggtctgctg ttggggatat tgctggcacg aaccgttgcc
    11701 gggttactgg caaacctcgg cggctggcgc accgtctttt gggttgcttc ggtgttaatg
    11761 gcactgatgg cgctggcatt atggcgtggt ctgccacaaa tgaaatcaga aacccacctc
    11821 aactacccac agttgttggg ttccgttttc agcatgttca tcagcgataa gatcctgcgc
    11881 acccgcgcgt tgctgggctg catgaccttt gccaacttca gcattctctg gacctcaatg
    11941 gcctttttgc ttgccgctcc accttttaac tacagcgatg gtgacattgg tctgtttggg
    12001 cttgcgggag ctgccggagc gttgggcgct cgtccggcgg gcggttttgc cgacaagggc
    12061 aaatcgcacc acaactttcg gtctgcttct gctgttactt tcatggctgg caatctggtt
    12121 tggtcacact tccgtactgg cgttgattat cggcattctg gtgctggacc tcaccgtaca
    12181 gggcgtgcat atcactaacc agacggtaat ttatcgaata catcctgatg cgcgtaatcg
    12241 cctgaccgca ggttacatga ccagctactt tattggcggt gccgccggtt cgctaatttc
    12301 agcctcagcc tggcaacatg gcggttgggc tggcgtttgt ctggctggcg cgacgattgc
    12361 cctggttaac ttactggtct ggtggcgagg ttttcatcgt caggaagccg caaattaagc
    12421 aatcagcaac cgttttcgtg gctttacact tataagggtg ttaagaagcc catcagtctg
    12481 ataaggttaa gatattcatt cagtctattt ataatattaa caatcgttaa gcgtacactc
    12541 tatggaaagc cctactccac agcctgctcc tggttcggcg accttcatgg aaggatgcaa
    12601 agacagttta ccgattgtta ttagttatat tccggtggcc tttgcgttcg gtctgaatgc
    12661 gacccgtctg ggattctctc ctctcgaaag cgtttttttc tcctgcatca tttatgcagg
    12721 cgcgagccag ttcgtcatta ccgcgatgct ggcagccggg agtagtttgt gggttgctgc
    12781 actgagcgtc atggcaatgg atgttcgcca tgttttgtat ggcccgtcac tgcgtagccg
    12841 tattattcag cgtctgcaaa aatcgaaaac cgccctatgg gcgtttggcc tgacggatga
    12901 ggtttttgcc gccgccaccg caaaactggt acgcaataat cgccgctgga gcgagaactg
    12961 gatgatcggc attgccttca gttcatggtc atcgtgggta tttggtacgg taataggggc
    13021 attctccggc agcggcttgc tgcaaggtta tcccgccgtt gaagctgcat taggttttat
    13081 gcttccggca ctctttatga gtttcctgct cgcctctttc cagcgcaaac aatctctttg
    13141 cgttaccgca gctttagttg gtgcccttgc aggcgtaacg ctattttcta ttcccgtcgc
    13201 cattctggca ggcattgtct gtggctgcct cactgcgtta atccaggcat tctggcaagg
    13261 agcgcccgat gagctatgag gttctgctgc ttgggttact ggttggcgcg gcgaattatt
    13321 gcttccgcta tttgccgctg cgcctgcgtg tgggtaatgc ccgcccaacc aaacgtggcg
    13381 cggtaggtat tttgctcgac accattggca tcgcctcgat atgcgctctg ctggttgtct
    13441 ctaccgcacc agaagtgatg cacgatacac gccgtttcgt gcccacgctg gtcggcttcg
    13501 cggtactggg tgccagtttc tataaaacac gcagcattat catcccaaca ctgcttagtg
    13561 cgctggccta tgggctcgcc tggaaagtga tggcgattat ataacccaca agaatcattt
    13621 ttctaaaaca atacatttac tttatttgtc actgtcgtta ctatatcggc tgaaattaat
    13681 gaggtcatac ccaaatggat agttcgttta cgcccattga acaaatgcta aaatttcgcg
    13741 ccagccgcca cgaagatttt ccttatcagg agatccttct gactcgtctt tgcatgcaca
    13801 tgcaaagcaa gctgctggag aaccgcaata aaatgctgaa ggctcagggg attaacgaga
    13861 cgttgtttat ggcgttgatt acgctggagt ctcaggaaaa ccacagtatt cagccttctg
    13921 aattaagttg tgctcttgga tcatcccgta ccaacgcgac gcgtattgcc gatgaactgg
    13981 aaaaacgcgg ttggatcgaa cgtcgtaaaa gcgataacga tcgccgctgc ctgcatctgc
    14041 aattaacgga aaaaggtcac gagtttttgc gcgaggtttt accaccgcag cataactgcc
    14101 tgcatcaact ctggtccgcg ctcagcacaa cagaaaaaga tcagctcgag caaatcaccc
    14161 gcaaattgct ctcccgtctc gaccagatgg aacaagacgg tgtggttctc gaagcgatga
    14221 gctaacgcgt catctcgctc aaaaatccag atttataaaa gaaaaaatga ccggccagca
    14281 tcgcaacatg ctggcctttt tggcaagcag gtcggctcag ccgatgagtt aagaagatcg
    14341 tggagaacaa tatgagcgca aatgcggaga ctcaaacccc gcagcaaccg gtaaagaaga
    14401 gcggcaaacg taagcgtctg ctcctccttc tcaccttgct ctttataatt attgccgtag
    14461 cgatagggat ttattggttt ttggtactgc gtcatttcga agaaaccgat gacgcatacg
    14521 tggcagggaa tcagattcaa attatgtctc aggtgtctgg cagcgtgacg aaagtctggg
    14581 ccgataacac cgattttgta aaagaaggcg atgttctggt cactctcgac ccgacagatg
    14641 ctcgccaggc gtttgaaaaa gccaaaactg cactggcttc cagcgttcgc caaacccacc
    14701 agctgatgat taacagcaaa cagttgcagg cgaatattga ggtgcaaaaa atcgccctcg
    14761 cgaaagcaca aagcgactac aaccgccgtg tgccgctggg caatgccaac ctgattggtc
    14821 gcgaagagct gcaacacgcc cgcgacgccg tcaccagtgc ccaggcgcaa ctggacgtcg
    14881 ctattcaaca atacaatgcc aatcaggcga tgattctggg gactaaactg gaagatcagc
    14941 ctgccgtgca acaggctgcc accgaagtac gtaacgccta gctggcgctg gagcgtaccc
    15001 gtattgtcag tccgatgacc ggttatgttt cccgtcgtgc cgtgcaaccg ggtgcgcaaa
    15061 ttagcccaac gacgccgctg atggcggtcg ttccagccac caatatgtgg gtggatgcca
    15121 actttaaaga gacgcagatt gccaatatgc gcatcggtca gccggtcacc atcaccaccg
    15181 atatttacgg cgatgatgtg aaatacaccg gtaaagtggt tggtctggat atgggcacag
    15241 gtagcgcgtt ctcactgctt ccggcgcaaa atgcgaccgg taactggatc aaagtcgttc
    15301 agcgtctacc tgtgcgtatc gaactggacc agaaacagct ggagcaatac ccgctgcgca
    15361 ttggtttgtc cacgctggtg agcgtcaata ccactaaccg tgacggtcag gtactggcaa
    15421 ataaagtacg ttcaacaccg gtcgcggtaa gtaccgcgcg tgaaatcagc ctggcacctg
    15481 tcaataaact gatcgacgat atcgtaaaag ctaacgctgg ctaatccaga ggtgcgtgtg
    15541 atgcaacagc aaaaaccgct ggaaggcgcg caactggtca ttatgacgat tgcgctgtca
    15601 ctggcgacat tcatgcaggt gctgaactcc accattgcta acgtggcgat ccccactatc
    15661 gccgggaatc tgggctcatc gctcagccaa ggaacgtggg taatcacttc tttcggggtg
    15721 gcgaatgcca tctcgatccc gcttaccggc tggctggcaa agcgcgtcgg ggaagtgaaa
    15781 ctgttccttt ggtccaccat cgcctttgct attgcgtctt gggcgtgtgg tgtctccagc
    15841 agcctgaata tgctgatctt cttccgcgtg attcagggga ttgtcgccgg gccgttgatc
    15901 ccgctttcgc aaagtctatt gctgaataac tacccgccag ccaaacgctc gatcgcgctg
    15961 gcgttgtggt cgatgacggt gattgtcgcg ccaatttgcg gcccgatcct cggcggttat
    16021 atcagcgata attaccactg gggctggata ttcttcatca acgtgccgat tggcgtggcg
    16081 gtggtgttga tgacactgca aaccctgcgc ggacgtgaaa cccgcaccga acggcggcgg
    16141 attgatgccg tggggctggc actgctggtt attggtatcg gcagcctgca gattatgctc
    16201 gaccgcggta aagagctgga ctgtttttca tcacaggaaa ttatcattct taccgtggtg
    16261 gcggtggtgg ctatctgctt cctgattgtc tgggagctga ccgacgataa cccgatagtc
    16321 gatctgtcgt tgtttaagtc gcgcaacttc accatcggct gcttgtgtat cagcctcgcg
    16381 tatatgctct acttcgacgc tattgttctg ctgccgcagt tgttgcagga ggtctacggt
    16441 tacacggcga cctgggcagg tttggcctct gcgccggtag ggattattcc ggtgattctg
    16501 tcgccgatta tcggccgctt cgcgcataaa ctggatatgc ggcggctggt aaccttcagc
    16561 tttattatgt atgccgtctg cttctactgg cgtgcctata cctttgaacc aggtatggat
    16621 ttttgcgcgt cggcctggcc gcagtttatc caggggtttg cggtggtctg cttctttatg
    16681 ccgctgacca ccattacgct gtctggtttg ccaccggaac gactggcggc ggcatcgagc
    16741 ctctctaact ttacgcgaac gctggcgggg tctatcggca cgtcgataac cacgaccatg
    16801 tggaccaacc gcgagtcgat gcaccatgcg cagttgactg agtcggtaaa cccgttcaac
    16861 ccgaatgccc aggcgatgta cagtcaactg gaagggcttg ggatgacgca acagcaggca
    16921 tcaggctgga ttgcccagca gatcaccaat caggggctga ttatttccgc caatgagatc
    16981 ttctggatgt cagccgggat attcctcgta ctgctggggc tggtgtggtt tgctaaaccg
    17041 ccatttggcg caggtggcgg cggaggcggt gcgcactaag tacaactaag ccagttcatt
    17101 tgaactggct tttttcaatt aattgtgaag atagtttact gactagatgt gcagttcctg
    17161 caacttctct ttcggcagtg ccagttcttc gttgctgttg atgcgtacct cacgttccag
    17221 aatgctacgc gcaatatcct gcgcttcctg caacgagtgc atctggtaag tgccacactg
    17281 gtagacgttc agttccggga tctgattctg atcctgcact ttcagcacgt cttccattgc
    17341 cgctttccag gcatcagcaa cacgctgctc atctggcgta ccaatcagac tcatataaaa
    17401 accggtgcgg cagcccattg gcgagatatc gataatctct acaccattac cgttaagatg
    17461 gttacgcata aaaccagcaa acaggtgctc cagggtatgg atccctcttt ctggcatcac
    17521 ttctttgttc ggcacgcaga agcgcagatc gaacacggtg attgcgtcgc catgcggggt
    17581 gttcattgtt ttcgccaccc gaactgcagg cgcttccatc cgggtatgat cgactgtgaa
    17641 gctatctaac aacggcattt agccacctcc ggtaattttt ttaaaaattt tctgaactct
    17701 ttcttcccag gcgagtctga gtatatgaaa gacgcgcatt tgttatcatc atccctgaat
    17761 tcagagatga aattttggcc actcccgagt ggcctttttc ttttctgtca ggcgtgtttt
    17821 tccagccaca ccgcaaacgg ttcggtatca gcggtttcca tttcctgctg acggcgttca
    17881 gatgcctcgc gctcggctac aaaatcctct tcgcgcagaa tttccagcgg ctcttcacgc
    17941 agcagattac ggtaggcttc tgcaaacgct ttgcctgttc cgccaatacc agtatcaatc
    18001 atagacctta agatacgggc agagaaagtc agatcgggat tatcgaagca ggcaaccagt
    18061 tcatcacaca ctttctgata cgcttcgccg ccgttaatgc tatccagcgt ttgcgcgacg
    18121 cgtttcagat cgcggaacag atctttaccc acctgcagta acgggaactg cgcggtttcg
    18181 cagccgatac ccagcgtcag gcccggtttg cgaccttcaa ggatcacccg gttccagtta
    18241 acgcgcgtac aggcaagttc gctactgctc atttccggtg catcagccag cgcacaccag
    18301 accataaaca ggtcgaggaa tcgcacctgc tgttcatcta caccaatcgg cgagaacggg
    18361 ttgatgtcca gcgaacgcac ttcaatatat tcaatgccgc cacgtaacag cgcatcagaa
    18421 ggtgactcgc cgctgcgggt aacgcgtttt ggacgaatcg gcgcatacag ttcgttttca
    18481 atctgcagca cgttgctatt gatttgcagc ctcttaccgt ctttctcaat accaatcttc
    18541 gcgtactctt ccgatggcgt tttgattgcc tgtttaaggc ccgctacgta ctcgtaaaga
    18601 tcgttgaagg taataccaag attgctttgc gatttattgg tatagccgag atcgctcaaa
    18661 cgaagagagg tcgcatacgg caggtaatac ataccgcact cggttttctc aaacggcagc
    18721 gacgttggtt ttccttgcag gaaagaagaa caaatcgccg gagatgcacc aaacagataa
    18781 ggaatgaccc aaccgaaacg atagtaattg cggataacgc ggaaatagcc cgcagaaatt
    18841 ttctctttgg catcagcgcc cgagatatca ccgcacttcg cttgccagaa tgccattggc
    18901 aaagagaaat tgtagtgcac gccggaaatg gtttgcatca gcgcgccgta gcgatttttc
    18961 agcccttcac gatacagcgt tttaaagcgt ccggtgttag aagtgccgta ctgtgccagt
    19021 tcgatgtcct gaccttctgc gatgtagcat ggcatactta acggccacat ccgctcatcg
    19081 cccatattgc gcgccgtata acgatgcaga tcgcgcataa aggtcagcat atgttcaata
    19141 tcaccatcca ctggtgtaat gaattccagc aatgcttccg caaaatcggt agtaatccat
    19201 ttgtgcgtca gtgcggaacc taatgcttca ggatgacctg ttgttgccag tgtgccatca
    19261 gcattaacac gcaaagtttc gcgctccagc ccacgctgta tcccctttaa cgcctgagga
    19321 tgtttttcca gccaggccag cgcctgtgat acgtccggga tcaaattgac ctcccgcctg
    19381 tcaaaatcgt tttaattagc ataactgtaa tggtgaccat atgtgcaggc ctacaattag
    19441 tgccaccaca tcatgccctg aacggtcgct gctgcaactg caacatagcg taacgcttta
    19501 ccaaggcaca aaaaaaagat tactggcccc caggagatgc gcatccatcc cgctaacaga
    19561 cacagtaaat cgccaaccac gggcatccag cttaataata gcgtgactgc accatagcgt
    19621 ttcagccagc cggtagcttt ctcttgccag cgcgatgttt tacgcaatgg aaagaaacgc
    19681 ccaaggataa cgttagttaa ccctccaagg ctattaccca ttgttgctgt taaaactaaa
    19741 acccagggat gactgatccc ggaaagcaac attgccacca gcacgacttc ggagttgccg
    19801 ggtaatagtg tagcgctgag aaaactactg gcaaacaacg agaaaagcga taacgcttca
    19861 ctcacagcaa gcgaacatcc acagcgtcca tgcctgctgc acgggccgcc tgaataccga
    19921 aatcggcatc ttcaaagacc acacactgcg tcggttgcac gcccatacgc tgcgcgcaca
    19981 acaaaaatgt gtctggcgcg ggtttatggt gtttgacgtg atcagcagcg acgacggcgt
    20041 caaaataacg gcgtaatccc aggtgcgcca gcaatgcctc agcgatggcg ctttcactcc
    20101 ccgttcctac agccattggg cgacgaccat gccaactttt caccacttca acaagaggaa
    20161 gcggctcgac actatccagc agcatacttc ttactgcttc tgttttttca cgcgctaacg
    20221 catgcgggtc gagatcggcc tgattcagct caataattgc ctgagcaata cgccaggtgg
    20281 gcgatccatt aagcgcaatc atcgcctgaa tatcgtactg aagaccgtag tgccctaata
    20341 cttcgcgcca cgctttacgg tgcgtaggct ccgtatccag gattgtgcca tccatatcaa
    20401 aaattaaacc tgcataacgc tcgtacatgg tcttctcgca agtcgaaaaa tatgacgcta
    20461 ctttagcgta attgcttgat tttgtcgctg atggcgaagg agaagaaaat tcaggaaatg
    20521 gataaagtag ttatttagaa atgagatatt tttgaaggaa attttttgga gaatatggtg
    20581 catccgggag gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg
    20641 agctacggat acatcgggaa acttacttta ctgcagattt tttgataccg ctactaaagc
    20701 cgtatcaagt aagagatggt gcatccggga ggattcgaac ctccgaccgc tcggttcgta
    20761 gccgagtact ctatccagct gagctacgga tgcatcggga aacttacttt actgcagatt
    20821 ttttgatacc gctactaaag ccatatcaag taagagatgg tgcatccggg aggattcgaa
    20881 cctccgaccg ctcggttcgt agccgagtac tctatccagc tgagctacgg atgcatcggg
    20941 aaacttactt tactgcagat tttttgatac cgctactaaa gccgtatcaa gtaagagatg
    21001 gtgcatccgg gaggattcga acctccgacc gctcggttcg tagccgagta ctctatccag
    21061 ctgagctacg gatgcaaatg gcggtgaggc ggggattcga accccggatg cagcttttga
    21121 ccgcatactc ccttagcagg ggagcgcctt cagcctctcg gccacctcac cacacgcctc
    21181 ttacgagtgc ttcgaagaac ttgtttatcg ctcatcatcg ctgcgtggcg cacatattac
    21241 tttctgagac ttataagtca aacaattttt cccacacttt tatcgtttgc acacttcacg
    21301 ctcaattagt ctgtaaaagc gtcaaaaaga gtgttttatc tacagaaaaa cggaggtctg
    21361 gcagatagca gtaatgcaaa aaaatggaga cttaagttga atgaacggga gtaaagcgaa
    21421 aagactatag agtgaaggag aaattttgag ggtgcgtctc accgataaag atgagacgcg
    21481 gaaagattag taactggact gctgggattt ttcagcctgg atacgctggt agatctcttc
    21541 acggtgaaca gaaacttcct tcggggcatt tacgccaata cgtacctggt tgccctttac
    21601 ccctaaaact gtcacggtga cctcatcccc aatcatgagg gtctcaccaa ctcgacgagt
    21661 cagaatcagc attctttgct ccttgaaaga ttaaaagagt cgggtctctc tgtatcccgg
    21721 cattatccat catataacgc caaaaagtaa gcaatgacaa acacattaca tgtaagcagt
    21781 catggcatta cattctgtta aacctaagtt tagccgatat acacaacttc aacctgactt
    21841 tatcgttgtc gatagcgtta atgcgaatgc cgtgaagcga gtccacggca ttgcctgacg
    21901 cttatattat tgcaatttcg cgctgaccca gcctttcaca ctggctaacg ctgcaggtaa
    21961 ggccgcagca tccgtaccac cggcttgcgc catgtcagga cgtccaccac ccttgccgcc
    22021 cacctgctga gcgaccatac caatcagttc ccctgctttc acacgatctg tgacgtcctt
    22081 agatacgcct gcaatcagag aaaccttacc ttcggctacc gttgccagca cgataattgt
    22141 cgaccccagc tgatttttta aatcgtcaac catggtacgc aacattttcg gctcaacacc
    22201 gctaagctcg ctaaccaaca gcttaacacc attaacatca attgccttac tggaaagatt
    22261 tgcgctctcc tgtgcggcag cttgttcttt aagctgttgt agttcttttt ctaactgacg
    22321 cgtacgttcc agtactgagc gcactttatc cgccagatta ttgctatcgc ctttcagcag
    22381 atgcgcgact tcgcttaagc gatcgctgtc tgcatgaacg gtggtgatag cgccttctcc
    22441 ggttaccgct tcgatacgac gaacgcctgc agcagtaccc gattcagaga tgatgcggaa
    22501 cagaccaata tcaccagtgc ggctggcgtg agtaccgcca cacagctcgg tggagaaatc
    22561 gcccatgctc agcacgcgta cacgctcatc atacttctcg ccgaacagcg ccatcgcacc
    22621 tttcgctttc gccgcttcga gattcatgat gttggtttcg atcggcaaat tgcgacgaat
    22681 ctgtgcgttc accaggtctt cgaccgcacg aatctcttcc ggtttcatcg cttcgttgtg
    22741 tgagaagtcg aagcgcagca ccttgtcgtt aaccagtgaa cctttctgcg atacatgagt
    22801 ccccagaacc tggcgcagcg cagcgtgcat caggtgcgtt gcggagtgat tcagacgaat
    22861 acgggcgcga cgagcctcat caatatcagc ctgcacagcg tcgcccactt tcagagaacc
    22921 cgcagcaagt ttaccgatgt gaccaatcgc ctggccgtat ttctgcgtat cttccaccgc
    22981 gaaggagaag ttagcgcctt tcagttcgcc tttatcacca acctgaccgc cggattccgc
    23041 atagaatggc gtttgatcca gcacgaccac agcttcctgg cctgcattga tggcatcaac
    23101 cgctttacca tcaacaaaca gcgcggtcac tttgccgttc agttccagat ggtcatagcc
    23161 tttaaattca gatgcactat caacacggat cattgcgttg taatcggcac caaagccgct
    23221 ggcttcgcgc gcgcgacgac gctgctcttc cattgctgct tcaaaaccag cttcgtcaac
    23281 tttgatgttg cgctcacgac aaacatcagc cgtcaggtca accgggaagc cataggtgtc
    23341 gtacagacgg aaagccgttt caccatccag cgtatcacca gaaagttttg ccagctcttc
    23401 atccagcaac gccagaccgc gctccagagt acgggcaaac tgctcttctt cagtcttcag
    23461 cacctgctca acctgcgcct gctggcgttt caggtcttca cccgcagagc ccataacgtc
    23521 gatcagcgga ccaaccagtt tgtagaagaa ggtttctttc gcgccgagca tattgccgtg
    23581 acgcactgcg cgacgaatga tacgacgcag tacataacca cggttttcat tggacggcat
    23641 tacgccatcc gcgatcagga acgcacaaga acgaatgtgg tcagcgatta cgcgcagcga
    23701 tttattgctc agatcggtcg cgccagtgac tttcgctacc gcctggatca acgtgcggaa
    23761 caggtcgatg tcatagttag agttaacgtg ttgcagcacc gcagcaatac gctccagacc
    23821 cataccggta tctacagacg gcttcggcag cggttccatc gtgccatctg cctggcggtt
    23881 gaactgcatg aagacgatgt tccagatctc aatgtagcgg tcgccgtctt cttccggact
    23941 tcccggaggg ccaccccaaa tgtggtcgcc gtgatcgtag aagatttcgg tgcacgggcc
    24001 gcacggacca gtgtcaccca tctgccagaa gttgtcagat gcgtatggcg cacctttgtt
    24061 atcgccgatg cgaataatac gttcgcgcgg gatcccgact tctttttccc agatttcgta
    24121 ggcttcgtcg tcgctttcat agacggtaac ccacagacgc tctttcggca gggcaaacca
    24181 tttttcgctg gtcagcagtt cccatgcaaa ctgaatggca tcgtgtttga aatagtcgcc
    24241 gaagctgaag ttgcccagca tttcgaagaa ggtatggtga cgcgcggtgt aaccgacgtt
    24301 ttccaggtcg ttgtgtttac cacccgcacg cacgcagcgt tgggaagtgg tagcgcggga
    24361 ataattacgc ttgtcgagcc caaggaacac atccttgaac tggttcatcc cggcgttggt
    24421 aaacaacaaa gttgggtcgt tatggggtac cagggagctg ctggcaacta cctgatgtcc
    24481 cttactatgg aaaaagtcga gaaacgcctg acggatctca gcggtgctct tgctcataat
    24541 tatcctgaaa tcaagctaac gaaatatcgc caccagctcc agcgtgtctt aaccgccggg
    24601 ctggtaactg aaaagtggga ataagataag ttttcttgac tgggaagtaa aataccgtat
    24661 gcgttcagtc ggcaaaattt cgccaaatat cctggatatc ttccatcaga tagccacgat
    24721 agagcagaaa acgctggatc ttaacttttt ctgaaaagac agttggcaaa ggttcaccat
    24781 attttcgcgt cgcctgatcg cgcgccagtg cgcaccagtc gatgtcacat tcacgcatcg
    24841 ctttttctgt cgcttcgcgg gaaatacctt tctgattcag ttcctgacga atacgtgcag
    24901 gtccataccc tttgcggcta cggctggcga taaagcgcgc aacaaatcgg ctgtcatcga
    24961 gatagccatg ttcatggcac caggcaataa cgcgctcgta atcttctgcc gtagcatcaa
    25021 tctcttctgg gccatttttg cccataatcg gtgccgcgag tttacgtcgc agttcttgct
    25081 cactgtgatc gcgcaccgcc agaatgcgta ccgcacgatc caacaggcga gcatatgccg
    25141 ggcgacggga tgttgattct gtcatggcat atccttacaa cttaaaaaag caaaagggcc
    25201 gcagatgcgc cccaggtttt gctgataaac agatttaata gtcaatgagt atgcagaatg
    25261 ttcccgcagg cccaattcgt tagaggatcg aggtgagagt aatttttcac gccagtgctg
    25321 gcctgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtc cgtgcacaca
    25381 ttatctatta tcatcaataa cgaacgatta aaaatcttcg ttagtttctg ctactccttc
    25441 gctgtcatct acagagaaat ccggcgttga gttcgggttg ctcagcagca actcacgtac
    25501 tttcttctcg atctctttcg cggtttccgg gttatctttc agccaggcag ttgcattcgc
    25561 tttaccctga ccgatcttct cacctttgta gctgtaccac gcgcctgctt tctcgatcag
    25621 cttctctttt acgcccaggt caaccagttc gccgtagaag ttgatacctt cgccgtagag
    25681 gatctgaaat tcagcctgtt taaacggtgc agcgattttg ttcttcacca ctttcacgcg
    25741 ggtttcgcta cccaccacgt tttcgccctc tttcaccgcg ccgatacgac ggatgtcgag
    25801 acgaacagag gcgtagaatt tcagcgcgtt accaccggta gtggtttccg ggttaccgaa
    25861 catcacacca attttcatac ggatctggtt gatgaagatc agcagcgtgt tggactgctt
    25921 caggttaccc gccagcttac gcatcgcctg gctcatcata cgtgccgcaa ggcccatgtg
    25981 agagtcgccg atttcgcctt cgatttccgc tttcggcgtc agtgccgcca cggagtcaac
    26041 gacgataacg tctactgcgc cagaacgcgc cagggcgtca cagatttcca gtgcctgctc
    26101 gccggtatcc ggctgggagc acagcaggtt atcgatatcg acgcccagtt tacgtgcgta
    26161 gattgggtcc agcgcgtgtt cagcatcgat aaacgcacag gttttacctt cacgctgcgc
    26221 tgcggcgatc acctgcagcg tcagcgtggt tttaccggaa gattccggtc cgtagatttc
    26281 gacgatacgg cccatcggca gaccacctgc cccaagcgcg atatccagtg aaagcgaacc
    26341 ggtagagatg gtttccacat ccatggaacg gtcttcaccc aggcgcatga tggagccttt
    26401 accaaattgt ttctcaatct ggcccagtgc tgccgccaac gctttctgtt tgttttcgtc
    26461 gatagccatt tttactcctg tcatgccggg taataccgga tagtcaatat gttctgttga
    26521 aacaattata ctgtatgctc atacagtatc aagtgttttg tagaaattgt tgccacaagg
    26581 tctgcaatgc atacgcagta gcctgacgac gcaccgcatc acggtcgccg ctgaagcatt
    26641 cccgccgggt aatgccttca ccgcgggcag tggcaaaagc aaaccagacg gtgccgacag
    26701 gcttctcttc actgccgcca tccggcccgg cgataccact aatagacacg gcataatcag
    26761 cacgagccgc tttcagtgca cctatcgcca tttccaccac gacgggttca ctcaccgcgc
    26821 catgctgcgc cagcgtctct tcgcgtacgc cgatcatctg cgctttggct tcgttactgt
    26881 aggtgacaaa tccgcgttca aaccaggcgg agctaccggc aatatcggta atcactttcg
    26941 ctacccaacc accggtacaa gactcggcag ttgttacggt tgcgccacgg gctttcagcg
    27001 cctgcccaac ctgttcgctt aactgcatca gttcactgtc agtcatcaca acctctgtca
    27061 gtcaaaaatt tatgccggac aagatagcac tttcgtcagg aagatggtgt tggcgttgca
    27121 attttacgag gaggattcag aaaaaagctg attagccaga gggaagctca cgcccccctc
    27181 ttgtaaatag ctactgtact cgcgccagcg ccacggcttg tcctaactgc cagaccgcca
    27241 ttgcgtaatg ggtgctgtgg ttgtaacggg tgatggtgta gaagttcggc agaccgtacc
    27301 agtactggta gccggtgcca acatccagac gcagcaggct ggcttgttga tggttgccca
    27361 gcggctgctg tggcgttaaa cctgcggcgg caagctgcga aatgctgtac ttagttttga
    27421 aaccatttgg caagcctgga gcctgaccgt ttgccattat cgcgacctga tcgcctttca
    27481 cccagccgtg cgctttgaaa tagttcgcca cgctaccgat cgcatcaacc ggatcccaca
    27541 ggttgatatg cccgtcgccg ctgaaatcta ccgcatattg tttgtaagac gacggcataa
    27601 actgtccgta gcccatcgcc ccggcaaagg aacctttcag attgagcgga tcgtcctgct
    27661 cgtcgcgcgc catcagcagg aaggtttcca gctcgccaga gaaatactcc gcgcggcgtg
    27721 ggtagttaaa tgacagcgtt gccagcgcat cgaggatgcg agttttcccc atcacgcgcc
    27781 cccagcgggt ttcaacgccg ataatcccga cgataatttc cggcggtacg ccatacacct
    27841 gccacgcgcg attcaacgca tcttcatact gattccagaa aaccacaccg ttctgcacgt
    27901 tgtccggcgt aataaatttt ttgcgataac gcagccatgc gccgttcgga cctgatggcg
    27961 gtttcaccga tgtggttggt gcctggttat ccatcagccg cagtaccgaa tccagacgct
    28021 tcgcctggga gagaatttcc tgcaactgct gacgatcgaa accgtgtttg ttcaccattt
    28081 tgtcgatgaa ctgctgggca ttcgggttat tagcgaaatc gccgcccatc tgcatcacat
    28141 tgtgctgcgg ctcaagcagg aagccgccag acggcgttcc ggtggtcgtt tcagtctcag
    28201 taggttttgg cttgctgcta caggcggcaa gcaacacaaa aaggggaagc aatgttacat
    28261 aacgacgctt gaacatgagg ggtccattta acagattcaa ccaggggcaa gtatggtaaa
    28321 gcatcacacc ccgcacaagg aagcggtagt cactgcccga tacggacttt acataactca
    28381 actcattccc ctcgctatac ttttattcaa actttcaaat taaaatattt atctttcatt
    28441 ttacaatcaa aataacactt ttaaaccttt caacctgatt agattaggtt gccgtttggt
    28501 aataaaacaa taaatcctga aggagagaac aatgatagaa accattactc atggtgcaga
    28561 gtggtttatc gggctgttcc aaaagggcgg agaggtgttt accgggatgg tgaccggcat
    28621 tcttccgctg ttgattagcc tgcaggttat catgaacgca ctgattaatt ttatcggtca
    28681 gcatcgtatt gaacgttttg ctcaacgttg cgccggtaac cctgtttccc gttacctact
    28741 gttaccgtgc attggcacgt ttgtcttttg caatccgatg accttaagcc tgggtcgctt
    28801 tatgccggaa aagtacaaac ccagctacta cgcggcggcc tcttatagct gccactcaat
    28861 gaatggcctc ttcccccata tcaaccctgc gaactgtttg tttatcttgg cattgccagc
    28921 ggtctgacaa cgctgaacct gccacttggc ccactggcgg tgagttatct gctggttggt
    28981 ctggtcacca atttcttccg cggctgggtg accgatctga ccaccgccat ttttgagaaa
    29041 aagatgggca ttcaacttga acaaaaagtt cacctggcag gagcaacatc atgacgcgta
    29101 ttcggatcga aaaaggaacg ggtggctggg gcggcccgct tgagctggaa gccacgccgg
    29161 gcaaaaaaat cgtctatatc accgccggta cccggcctgc gattgttgac aaactggcac
    29221 aacttactgg ctggcaggct attgacggat ttaaagaagg tgaacccgcg gaggcggaaa
    29281 ttggtgtcgc ggtaatcgac tgtggcggca cattacgctg cggcatctat ccgaaacgac
    29341 gtattcccac cattaatatc cactcgacgg gcaagtccgg tccgctggcg cagtacattg
    29401 tggaagatat ttatgtctct ggcgtaaaag aagaaaacat cactgtagtg ggtgatgcga
    29461 caccacaacc ctcttccgtg ggccgtgact atgacaccag taagaaaatc accgaacaaa
    29521 gcgatggttt actggcgaag gtgggaatgg gcatggggtc cgccgttgcc gtgttgtttc
    29581 agtctggtcg tgacaccatc gacactgtat taaaaaccat tctgccgttt atggcattcg
    29641 tctcggcgct cattggcatc attatggctt ctggccttgg tgactggatt gcccacggtc
    29701 ttgctccgct agcgagccat ccactgggtc tggtcatgct agcgctcatc tgctccttcc
    29761 cactgctttc acctttcctc ggcccaggcg cagttatcgc acaggttatc ggcgtattga
    29821 ttggcgtgca gattggtctc ggcaatattc cgccgcatct ggctttaccg gcactgtttg
    29881 ccatcaacgc gcaggcggct tgcgacttca tcccggtcgg tttgtcgctg gcggaagccc
    29941 gtcaggacac ggttcgcgtc ggtgtccctt ctgtactggt gagccgcttt ttaaccggcg
    30001 cgccgactgt actgatcgcc tggtttgtct ccagttttat ctatcaatag aggctgaaac
    30061 atgaccgtta tttatcagac caccatcacc cgtatcggcg cgagtgccac tgacgccctc
    30121 agcgaccaga tgctcatcac ctttcgtgaa ggcgcgccag cggacctcga agagtattgc
    30181 ttcattcatt gccacggcga gttgaaaggt gcactccatc ccggtttgca attttcactc
    30241 gggcagcatc gctatccggt gaccgctgtt ggcagcgtgg cggaagacaa ccttcgcgaa
    30301 ctgggtcatg tcaccctgcg cttcgatggt ttaaacgaag cggaatttcc gggcactgtc
    30361 catgtggcag gccctgtacc cgacgatatc gcgccgggat cggttttgaa gtttgaatct
    30421 gttaaggagt aaaaaatgaa tcaggttgcc gttgtcatcg gtggtgggca aaccttaggc
    30481 gcgttcctgt gccacggtct ggctgccgag gggtatcgcg tcgcggttgt cgatattcag
    30541 agcgacaaag ccgcaaatgt ggcacaagaa attaacgccg aatatggtga aggtatggcg
    30601 tacggttttg gtgctgacgc cactagcgag caaagcgttc tggcgctctc tcgtggggta
    30661 gatgaaatct ttggtcgcgt ggatttgctg gtctatagcg ccggaatagc aaaagcagcc
    30721 tttatcagcg acttccagct cggcgatttt gaccgttcgc tacaagtgaa tctggtgggt
    30781 tatttcctgt gtgcccgtga attttcgcgt ttgatgatcc gcgacgggat tcagggtcgc
    30841 attattcaga tcaactcgaa atccggcaaa gtgggcagca aacacaactc tggctacagc
    30901 gcagcgaaat ttggtggcgt cgggctgact caatcactgg cgctggatct ggcggagtac
    30961 ggcattacgg tacattcact gatgctcggt aacctgctga aatcgcggat gttccagtca
    31021 ctgttgccac aatacgcgac caagctgggt atcaaaccgg atcaagtcga gcagtattac
    31081 atcgacaaag taccgctcaa acgcggctgc gattatcaag atgtgctgaa tatgctgctg
    31141 ttctacgcca gtcctaaggc gtcgtactgc accggacagt cgatcaatgt caccggcggt
    31201 caggtgatgt tctgatcaac agcggagatc cgttaaggat ctccgtgaga ctatagaatg
    31261 cctgatgcgc tacgctcatc aggcatacag gacttccgcc actacattaa ggaaaagtta
    31321 tggtatccgc actcatcacc gtcgccgtta tcgcctagtg tgcgcaactg gccttaggcg
    31381 gctggcaaat ttctcgtttt aaccgtgcct tcgacacact atgccagcaa gggcgggttg
    31441 gcgtgggccg ttccagcggg cgctttaaac cgcgggtcgt ggtcgccatc gcgctggacg
    31501 atcagcagtg catcgtcgac accttgttta tgaaaggact gactgtcttc gcccgaccgc
    31561 aaaaaattcc cgcaattacc ggcatgcatg cgggtgattt acagcccgat gtgatctttc
    31621 cccatgatcc actatcacag aatgctctat cattggcgct taaactgaaa cgtggataat
    31681 ttcgttgtga atgttacttg cttgcgaagt tatcattttg aaacctaaat caggtaatca
    31741 cgcccatgaa acctcgtcag cgtcaggccg ccattctgga gtatctgcaa aagcagggta
    31801 aatgctcggt tgaagaattg gcgcaatact ttgacaccac aggcacaacc attcgcaaag
    31861 atctggtcat tctggaacat gccggaaccg tcattcgtac ttatggcgga gtggtgttga
    31921 ataaagagga atccgatccg cctatcgatc ataaaacgct catcaacacc cacaagaaag
    31981 agctgattgc agaagctgcc gttagtttta tccatgatgg cgattcgatc attcttgatg
    32041 ctggcagtac cgttttgcag atggttcccc tgctctcgcg ctttaataac atcacggtga
    32101 tgaccaacag cctgcatatc gtcaatgcgc tatccgaact ggataacgaa caaactatcc
    32161 tgatgccagg cggaacattt cgcaaaaaat cggcctcatt tcacgggcaa ctggcagaga
    32221 atgccttcga gcatttcacc ttcgataaat tgtttatggg caccgacggc atcgatctca
    32281 atgcgggcgt aaccacgttc aacgaggttt ataccgtcag taaggcaatg tgcaatgccg
    32341 cgcgcgaagt gattttgatg gcggactcat caaagtttgg ccgtaaaagc cccaacgtag
    32401 tttgcagtct tgaaagcgtc gataagctaa ttaccgacgc aggtatcgat ccggcgtttc
    32461 gtcaggcgct ggaagagaaa gggatcgatg tgatcataac cggagagagc aatgagtgaa
    32521 gcactactga acacgggacg tcagacgtta atgctggagt tgcaggaagc aagccgttta
    32581 ccggaacgtc tgggcgatga ttttgttcgc gccgccaata tcatcctgca ctgcgaaggc
    32641 aaagtggtgg tttcgggaat tggcaaatcg ggccacattg gtaagaaaat cgccgcaaca
    32701 cttgccagta ccggcacacc ggcttttttt gtccatccgg cagaagcact gcacggcgat
    32761 ctggggatga ttgaaagccg cgatgtgatg ctgtttatct cttactccgg tggcgcgaag
    32821 gaactggatc tgattattcc gcgtctggaa gataaatcta tcgcgctgct ggcgatgacc
    32881 ggcaaaccga cgtcaccgct gggcctggcg gcgaaagcgg tgctggatat ctccgtagaa
    32941 cgcgaagcct gcccgatgca ccttgcgccg acctccagca ccgtcaatac cctgatgatg
    33001 ggtgacgcgc tggcgatggc ggtcatgcag gcgcgcggtt ttaatgaaga agattttgcc
    33061 cgctcccacc cagctggggc actgggcgct cgcttgctga ataaagtgca tcatctgatg
    33121 cgtcgtgatg acgctatccc acaggtagcg ttaaccgcca gcgtgatgga tgcgatgctg
    33181 gaactcagcc gcaccggtct ggggctggtg gcggtatgtg acgctcaaca acaggtacaa
    33241 ggcgtcttta ccgacggcga tttacgtcgc tggctggtag gcggcggcgc actcaccacg
    33301 ccagttaatg aagcgatgac gacaggcggc accacattac aggcacaaag tcgcgccatc
    33361 gacgccaaag aagttctgat gaaacgcaaa atcactgccg caccagtggt agatgaaaac
    33421 ggcaaactca ccggcgcaat taacctgcag gatttctatc aggccgggat tatttaatcc
    33481 ttcaatccca gacgtttcgc cagccgatgc aggttggcga cgtcagtttc cagcattcgc
    33541 gcacaggctg cccagttgtg atgattttgt gccagtgcct ggcgaatagt ttcacgctgg
    33601 aacgcttctg tcgcttcacg caggttttgc ttaacaacga gcaccgccgc cgcttctggc
    33661 ggcggcaacg tcacctcagg aaaagcaaaa tgttgcgcct caagaatcac ttcatcgccg
    33721 ttgcgggtgg ctctcgccag cactaccgcc cgatgaatag catgttccag ttcgcgcacg
    33781 tttcccggaa aactgtagtg ttgcagtaaa tttcgcgccc cggcacttaa taccacgcgg
    33841 gagagcccct gccgcaaacg acactgctcg cagaaatacc ccgccagcag aatgacatca
    33901 tcgccccgct cacgcagcgg cggcaccgaa agtggaaaca cgctcaggcg atgaaacaaa
    33961 tcggcgcgga atcgccctgc cagcacctct tcgcgtaagt cgcggttagt cgccgccagc
    34021 acgcgcacat cgacccgcaa actacggtca tcgccaacgc gctgaatatc gccatactgc
    34081 aacaccctca gcagcttggc ctgcaatgcc aacgacaact cgccgatctc atcgagaaac
    34141 agagtaccgt tatccgccat ttcaaacttc ccgctgcggt tactgatagc gccagtaaac
    34201 gctcctttca catgcccgaa caactcactt tccgccacac tttccggcag tgcggcacag
    34261 ttgagataga ccagcggatt caccgcccgt ggcgaggctt catgaatcgc tttcgccacc
    34321 agctccttac cggttcccgt ctcaccgctg atcagaacgt tgagatcgga cgccgccaca
    34381 atctcaatct cttttttcag ttgcgtcatg ccaggggaca agccaatcat ctgcgtctgt
    34441 ttcaccgctt caaacggcgt ggcatcgcct ggcagcatat tctggctttc cagttgttca
    34501 atcagcaacg cattgcttaa cgctcccgcc gccagcgcgg caatcagccg taactcttcg
    34561 tcgctgaaaa catcgaactg atcgggctgc atcccgtcga gcgtcagtgc gccgatcagg
    34621 ttttgtccga caaacaatgg cagaccaacg caggcgtgaa ccttcagact ctcctgccca
    34681 ggaatcaaac cgtcataggg atcgggcaat tcgctgtctg cgggaaagcg caccacatcc
    34741 ccggcgcggg caatcgcttc cagccgtgga tgcccttcca gcgcaaagcg tctaccgagt
    34801 acatcctttg ccagaccgtc gatggcaagc ggaataaact gccgcgaatc gtaacgtagc
    34861 aacgcagacg catcgcactc cagcacctga cgtagcgtgg tgatcaggcg ctgaaaacga
    34921 tcctgatgac caatcccacg ctgcaattcg atggcgatat tcgccagcac atcaacggaa
    34981 aaactcatct ttgcctcact gtcaatttga ctatagatat tgtcatatcg accatttgat
    35041 tgatagtcat tttgactact cattaatgag cataatttta tttatagagt aaaaacaatc
    35101 agataagaaa ctggcacgca atctgcaatt agcaagacat ctttttagaa cacgctgaat
    35161 aaattgaggt tgctatgtct attgtggtga aaaataacat tcattgggtg ggtcaacgtg
    35221 actgggaagt gcgtgatttt cacggcacgg aatataaaac gctgcgcggc agcagctaca
    35281 acagctacct catccgcgaa gaaaaaaacg tgctgatcga caccgtcgac cataaattca
    35341 gccgcgaatt tgtgcagaac ctgcgtaatg aaatcgatct ggcggatatc gattacatcg
    35401 tgattaacca tgcagaagag gaccacgccg gggcgctgac cgaactgatg gcgcaaattc
    35461 ccgatacgcc gatctactgt actgccaacg ctatcgactc gataaatggt catcaccatc
    35521 atccggagtg gaattttaat gtggtgaaaa ctggcgacac gctggatatc ggcaacggca
    35581 aacagctcat ttttgtcgaa acaccaatgc tgcactggcc ggacagcatg atgacttacc
    35641 tgacaggcga cgcggtgctg ttcagtaacg atgctttcgg tcagcactac tgcgacgagc
    35701 atctgttcaa cgatgaagtg gatcagacgg agcttttcga gcagtgccag cgttactacg
    35761 ccaatatcct gacgccgttc agccgcctgg taacgccgaa aattaccgag atcctgggct
    35821 ttaacttacc agtcgatatg atagcgacct cccacggcgt ggtctggcgc gataacccga
    35881 cgcaaattgt cgagctgtac ctgaagtggg ccgctgatta tcaggaagac agaatcacca
    35941 ttttctacga caccatgtcg aataacaccc gcatgatggc tgacgctatc gcccagggaa
    36001 ttgcggaaac cgacccacgc gtggcggtga aaattttcaa cgtcgcccga agcgataaaa
    36061 acgaaatcct gaccaatgtc ttccgctcaa aaggcgtgct ggtcggcact tcgacgatga
    36121 ataacgtgat gatgccgaaa atcgccgggc tggtggagga gatgaccgga ttacgcttcc
    36181 gtaacaaacg cgccagtgct ttcggatctc acggctggag cggcggtgcg atggatcgcc
    36241 tttccacgcg cctgcaggat gcgggtttcg aaatgtcgct cagcctgaaa gccaaatggc
    36301 gaccagacca ggacgctctg gagttatgcc gtgaacacgg tcgcgaaatc gcccgtcagt
    36361 gggcgctcgc gccgctgccg cagagcacgg tgaatacggt agttaaagaa gaaacctctg
    36421 ccacgacgac ggctgacctc ggcccacgaa tgcagtgcag cgtctgccag tggatttacg
    36481 atccggcaaa aggcgagcca atgcaggacg ttgcgccagg aacgccgtgg agtgaagtcc
    36541 cggataactt cctctgcccg gaatgctccc tcggcaaaga cgtctttgac gaactggcat
    36601 cggaggcaaa atgagcaacg gcattgtgat catcggttcg ggcttcgccg cccgccaact
    36661 ggtgaaaaat attcgcaaac aggacgccac tattccatta accctgattg ccgccgacag
    36721 catagatgag tacaacaaac ccgacctcag ccatgttatc agtcaggggc aacgtgccga
    36781 cgaccttacc cgccagacgg cgggtgaatt tgccgggcag tttaatctgc gcctgtttcc
    36841 gcacacctgg gtgacggata tcgatgccga agcccatgtg gtgaaaagcc agaataatca
    36901 gtggcaatac gacaagttag tgctggcaac cggtgccagc gcctttgtcc cgcctgtgcc
    36961 tgggcgtgag ttaatgctga cgttaaatag tcagcaagag tatcgcgcct gtgaaacgca
    37021 actgcgggat gcccgacgcg tgttgattgt cggcggtggt ttgattggta gcgaactggc
    37081 gatggatttt tgtcgtgcag gcaaagcggt cacgctaatc gacaacgctg ccagtattct
    37141 ggcgtcatta atgccaccag aagtaagcag ccgcttgcag catcggttga cggagatggg
    37201 cgttcatctg ctgttgaaat ctcagttgca ggggctggaa aaaacggatt ctggcattct
    37261 ggcaacgctg gaccaccagc gtagtatcga agtggatgcg gtaattgccg ccaccggact
    37321 gcgcccggaa acagccctgg cacgacgcgc cgggctgacg attaatcgcg gcgtttgcgt
    37381 cgatagttat ctgcaaacca gtaatgccga tatttacgcg ctgggcgatt gcgcggaaat
    37441 taacggtcag gtattgccgt tcctgcagcc gattcaactt agcgggatgg tgctggcaaa
    37501 aaatcttctc ggcaataaca cgccgctgaa actaccggcg atgctggtga aaatcaaaac
    37561 gccggaatta ccgctgcatc tggcaggcga aacccagcgt caggatttac gctggcagat
    37621 taataccgaa cgccagggaa tggtggcgcg cggcgttgac gatgctgacc agcttcgcgc
    37681 ctttgtggtc agtgaggatc ggatgaaaga ggcatttgta ttgttgaaaa cgttgccggt
    37741 gtaggtgggc tactgtgcct aaaatgtcgg atgcgacgct ggcgcgtctt atccgaccta
    37801 cggggacgca tgtgtaggcc ggataaggca tttacgccgc atccggcaag ggtgtccaaa
    37861 tgcaacacgc tttatccgtt ctggacttca cccgctaacc aacgcgccgc agcaataacc
    37921 ccctgcccca gagacaaacc gccatcaccc gccggtaaac tctgtggaaa gagcaatgtg
    37981 aaatcagcga gataatgcgc cagacgtgca cgcagcaaac ggttatgaat aaccccgccg
    38041 ctaaatacca gcgtagtgat accacgcatc gtggcctgct cacgcatcaa cgcggcaaaa
    38101 ccctgcgcca gcgcatcatg aaacgcccac gcgcgttgat taaccagtgc ctgccagttc
    38161 agccactgct gccagaaagt ggcgagatcc agttgattgt ccacccgcgg catcgtcacc
    38221 ggatgcgtca ctccgtcgca tgaggctgcg agcgcctcca gagcacaagc cgcttcacct
    38281 tcataactta acgtggctgg cgcacagccc agtgccgccg ccactgcatc gaacaaccgc
    38341 ccacacgatg atgccagcgg cgcgttaatt ccacgctcaa tggcccgcgc cagtacgctc
    38401 cagttttgct gttgcacact tgccgtttca gggtaattct gccactccgg cacaaagcgc
    38461 aggcactgcg ccagcaggtt tcgccacggc tgcttcgccg ccaaatcgcc acccggaagc
    38521 gccactgcag gcaagccgcc caggtgctca cattcgcgat agttcacccg caggcactcg
    38581 ccgccccaca aagcgccatt ctcccccata ccgataccgt cgagcgtcaa agcaatgaca
    38641 tcaccgccat ccagcggcca ctgatgctct gccagacacg ccgctgcatg ggcatgatga
    38701 tgcagtaccg tttgcgtcgg cagattcatt tcgctcgccc actggctgga gacatagccc
    38761 ggatgcacat catgcacaat gtattgcgga gtgaaatcgt agatgttttg catcaggcgt
    38821 aacgcttcgc gccactgcat ctggatgcca tcgtcactta aatcgcccag atgctgactc
    38881 aacaccgctt gttcaccgcg caccaggcag aaggtatttt tcagatccgc gccaagacac
    38941 agcacaggcg gaacattttt aaagcccgga ggcaaaacca gcgcatccgg cacatacccc
    39001 cgcgaacggc gcagcatttc gccgctttcg cgcaccactg aatcatccat ccgctgcacg
    39061 atgtcgcggt tatgtagcaa gaatccgtcg gcaatgccct gcaaatccgc cagcgcctgt
    39121 tcgttgctga tagctggtgg tttaccgctc aggttgccgg aggtcatcac cagcgggcat
    39181 tgcagttcct gtaacagcaa atgctggagc gggttcgcag gcaacatcac cccgacttcg
    39241 ttaaggccag gggcgatatc atcacaaagc tcaggtacgt attttttatc caccagcaca
    39301 atcggcgcag cgggcgtggt aagcaactgg cgcgcagcgt ctggtaaact ttctgccact
    39361 ggcaacatga ctgccagcgg tttcgccggg cgatgtttgc gctcccgcag tgtcgccacc
    39421 gcgttactgt tacgtgcatc gcaggcaaga tgaaatccgc caatcccttt gatggcgaca
    39481 atgttgccca tttttaactg tgcgatagct gcctgtaatg ccgcctcttg ttctgcatgt
    39541 tcaccatgac ttacccattc aagatgcggg ccacactccg ggcaggccac cggctgggcg
    39601 tggaagcgac gatcgagcgg gtcgcggtac tctttgtcac aggccggaca tagcggaaac
    39661 gccgccatca cggtaaacgg gcggtcgtaa ggcatcgcgc gaataatggt gaaacgcggg
    39721 ccgcagtggg tacagttgat aaacggataa cgataacgcc gctcgcctgg ggtattcatt
    39781 tcggcaaggc aagcagggca agtggcggca tccgggacaa tttgcgtatt catggtgccg
    39841 ccggtgctct ggcggatggt gaactcggtg ggcagttgtg accagataaa cggctcacgc
    39901 tcgacgctat caatacgcgc cagtggcgga cagtgctgat gcaattgaac aagaaacgtt
    39961 tccgggtctt ccagcagccg gacttctacg ccatcgccgt cattacatac atcgccgtga
    40021 agatttaatt gctgtgccag ctgccagaca aacggacgaa aaccgacgcc ctgcactttg
    40081 ccacgaatac gcagttggac accgcaagat gtgttttttg ccattgagtt attcccgcca
    40141 tcatgaattg cgtaacccgc cctgccggac acgacagcgt cgtatccggc agtctcaggt
    40201 cggcgatccc gcccgctccg tattctacga atatttccgg gaattccttt gatgccagaa
    40261 cagttctgta agatttttag aacatcagcg ccgtacggcg gcgtttttct gcgctcagtt
    40321 gttcaagttt attacgatcg acacaaatca gcgcatgggt cgggcaagcc gccatacacg
    40381 ccgggccgtc ttcacgatgg ttgcacaggt cgcatttatt ggcttcggct ttgtcagccc
    40441 gtacattcag acccgcgccg ctgttgcgaa tcacaggacg taccaccact tccatcgcgc
    40501 catacgggca agccaccacg caggttttgc aaccaatgca acgttcctgc atcacaggaa
    40561 caaacccttt atcacggctg atagcaccat tcgggcagac gttagcgcac ggtgcatctt
    40621 cacactgacg gcaaactgtc gccgtggaaa tgttcacacc tttaatgaca tggatacgcg
    40681 gtaaaaatgt ttccggggtc agcgatgcac agtcctgatt ttcctgatga gaaaccacgc
    40741 acgccacttc acaggtacgg caaccaatac atttactcgc gtcagcaatg atgaaacggt
    40801 tcatcaaatt ctccagcaat gacagttaat gcgccgatac attcacaaat catgccagtt
    40861 tttaatttgc tgttatttaa ggaaattaat ttctgtaatg caggaaaaaa gatgtcatcg
    40921 acactagtga cgatgacatg tgatgacaat gtttatcgcg aaagggcaat gagtgagtcg
    40981 cggcggatca gttttccgct aaaggttttc ggcggtgaga aatccccgcc atcgagcata
    41041 aaaatcagcc gtccaataat ttcctgaatc atctcagtca ccggaatttt tacgctggag
    41101 agcgccggaa cgatataggg ggcaattgcg atatcatcga atccgataac tgacacctgc
    41161 tctggcaccg ctacgccgcg ctcgtgcaac gctttgatcg cacctatcgc catatcgtcg
    41221 ttactggcaa ctaacgcgct aaatttagcc cctcgttcga gcaacgtttc taccccttcg
    41281 gccccgctgg cgggcgtcca tttaccgtta gcgataagtt tttcattgag cgcaatacca
    41341 tgctgcgaca gcgcgtcttt atacccggca agacgttcaa tgctggtggg ggaatccatc
    41401 gagccggtaa ggaaagcaat ctcctgatgc ccggcgttta tcaactctgc cacggcgtta
    41461 aagctggtct gtttatgatc gcaccagacg ctatggctgc tattattgcg caggcggcga
    41521 ttaagcacca ttatcggctg actgtgcgcg tcaatgatgt catcaatctc atccacgctt
    41581 aaaaaacgcg ggtaaatcat aatggcgtcg cagcgcaaat ccagcagata ctgaatcgcc
    41641 tggcgctctt cttcggcgct gtgtttacca tctgccaata gcaactgtcg ccctttctct
    41701 tccgccattc gcgcggcatg aaacagtaat tcactaaaat aaatgccgtg ataaagcgtg
    41761 ttggtcacta ccagccccag cgtctgagta ctcttcgccg acagattgcg ggccagcaaa
    41821 tttgggcggt aactgctctc ttctaccgcc tgaaacacgc gatctttagt ctcctggctg
    41881 acgtagccat tacctgaaag cacgcgggaa acggtcgctt ttgaaacccc ggcgcgcttc
    41941 gccacttcca gcatcgtcgt catcattttc atccctttac acgcaatcaa cgcagtgtac
    42001 tgcaccgttt gccgattgtc cttgcgcaat cggctccagc ggataagacg aggtcgccgt
    42061 gccggttttg atgacttttt tgataaaggt aaacatggcg attccttatt tcagcggcga
    42121 gtttttacgc tcaatgctgt aacgctcgag ttctttgtac ggcaccactt tgctcttctt
    42181 cttacgcaca tcgactacgg tcatgcggtc ggtacaggag tagcaagggt ccaggctacc
    42241 gataatcagc ggcgcatcgg aaacggtatt gccacgcagc atgtagcgca gggtcggcca
    42301 gttcgcgtag gtcgcggcac ggcagcgcca gcggtacagc ttctggttgt cgccggtcat
    42361 gctccagtgg atatcgtcgc cgcgcggcgc ttcggcaaag cccagcgcga aacggtgcgg
    42421 aatgtaggta aagccttcca ccatcagcgg accgcccggc aggttatcca gaccgtagtc
    42481 gatcatattc agcgcggtat agacctcgtt gattcgcact ttcagacggg aaataacgtc
    42541 gcagccctgc tcgctgtgga cttccattgg cagcaggcca taaccgacaa acgggtgatc
    42601 ggcgcgagta tcgcgggcga gaccgctggc gcgaaccatc gggccaacgt tactgaaatc
    42661 gcgggcgatt tccgggtcca gacgaccaat gccgacggtg cgttgttcca tgttcggcgt
    42721 actcagcagc acatccacca gctcctgcac ttcacgacgc atttgttgcg ccagctggcg
    42781 ggtctggatc atgtcatctt tcagcagatc gcgacgaatc ccgccgatca ggttcaggcc
    42841 gtaggtttta cgcgcaccgg taaggatctc tgccattttc atggaggttt cacgcacgcg
    42901 gaagaactgc ataaagccgg agtcgaagcc ggtaaagtga caggcgaggc cgaggttgag
    42961 cagatgcggg tgcaggcgtt ccacctccag cagaatggcg cggatcatct gcgcgcgttc
    43021 tggcacctga atacccatcg cgttttccac cgacgtggtg taggcggtgc tgtgggcaaa
    43081 gccgcagatc ccgcacacac ggtcagagag gaaggtcact tcgttataac ccatgcgggt
    43141 ttccgccagt ttttccatac cgcgatggac gtagaacaga cggtagtcgg cgtcgataat
    43201 gttttcgcca tcgacgaaca gacggaagtg acccggttcg tcagaagtga cgtgcagcgg
    43261 accaatcggc acgacgttgt ttttcttgtc gcccagttcg ttgatgaact cgtaggtttc
    43321 agcatcggta gtcggtgccg gacgctgacg ataatccatg ctgtctttac gcagcggata
    43381 aagttcatcc ggccagtcat ccggcagcac cagacgacgt tcatccggca gaccaaccgg
    43441 aatcaaaccg tacatatcgc gcacttcgcg ctcgccccac accgccgccg gaacgcgcgg
    43501 cgtcacggac ggatactccg gtttgttggc gtcaacttcg acgcgaaccg ttacccaaca
    43561 cttggtgccc ttctccatcg acagcacgta ataaacggcg taatgaccat tcagtttgcg
    43621 ttcgtcgtta ccaaacagca ccgacagcca gccaccctgc ttgtagtaga gaaactccac
    43681 cacttccggc agatagttca cctttacggt gacagtcagc tgatctttgg tctgccaggc
    43741 gtggtccagc acgacgccta gaaatgcctc attcagcgcg gcgagataat gttgacctaa
    43801 tttttcttca gacatgctca aactctcttt aatcacgccg ccagcaagga gacgaacgct
    43861 aaaaatgcaa agccaaaccc agcccaggta atgcgcggag taatatcaag acgcagacgg
    43921 gccatgctgt tttcgaacag cgcgataacc aggacgccga ccaccagttt gacgatggca
    43981 atcaccagcg ccagcagcag tccaccgacg gtgaaggttt ccatttgtcc ccacggaata
    44041 aacaccccga cgaacatctg caacaccacc agctgtttca ggctgatacc ccatttcatg
    44101 acgccaaagc cgctaccgct gtattcggag agcggacctt cctgcaactc ctgctcggct
    44161 tccgccaggt cgaacggcag tttgcccatt tcgataaaag tggcgaacgc acaggcgcag
    44221 agcgccagca ccagcgggat gctttggctc agcggccagt gataaacggt gtcggtaatg
    44281 ttgctgatgt tggtggaacc ggcaacctgt gcggcaaccc acagaccaag cagcaacatc
    44341 ggttcgacca gcacgccaag catcgcttca cggctcgcac cgatagcggt aaacgggcta
    44401 ccggtatcca gaccggaaat ggcaaagaag aaacgcgcga tggcgaagag atacagtaag
    44461 gtgatcaaat cgcccagttg cggcagcgga gaaccgacgg tcaccaccgg cagcgcagta
    44521 gcgatagtca gcatgacgcc caccatcaca tacggcgtca ggcggaacat ccagccagag
    44581 gcatccgggc caacgctctg gcggcccagc agtttgataa tgtcgcgata ctcctgcaac
    44641 acgcccggcc cgcgacggtt atgcagacgg gcgcgcgcca cgcgggtaat accggagagc
    44701 agcggcgcaa cggcaaataa caccagcgcc tgaattaacg gatataaaac actcattctc
    44761 aggctcctcg tgaaacaata atcaccacca gtaccgccag ttcaaccagc gccatccggc
    44821 ggaacagcaa cgcactacct tcgcactgcc agcccggcac tagcgacacc ggattcagcc
    44881 atttgcgcag tttcagcact ggcgcaaacg cctgtttaac cggcatggca aaaccgtgag
    44941 cggtaatcac cattgatttt tcatggtcgt agccgcacac ccaggccgca ccgcgggaac
    45001 gtgacggcag gcggtcgcct ttgcaaatcg ccataatgat gaatggcagc agcgggcagg
    45061 caatcagcag caatgtgatc attggttgag aaacggtggt gttagcaggc tccagaggca
    45121 gaggtacagc agcagagagc atcggcagca gccacggcgc agcaacaccg ccaattacgc
    45181 agcaaatcgc cagtgccact acgcttacgc tcatcaggag cggcgcacag gtggcgtttt
    45241 cggcttcttt ggtacgcggc gcgccgagga acgtgacgcc atagactttc gccatacaca
    45301 tcaccgccag cgcaccggta attgccagac ccacagcgag cagcggcccc agcagacggg
    45361 caacaaacgc gccgctattg ctcagtttga agaaggattg atagataacc cattccccgg
    45421 caaacccgtt cagtggcggc agcgcagcca ttgccatcaa cccgactaac atggcgatgg
    45481 agataaccgg cattttcttg ccaataccac cgagtttttc gatatcgcga tgaccggtac
    45541 ggaaccagac gctccccgcc ccgaggaaca gtacgctttt gaacaggcta tggttaagca
    45601 gatggtacag accaccgacc aggccaagag caatcagcgc cggttgttcg agcgcgatac
    45661 ccgttacgcc agcgcccagc cccagcccca gccccagcag gataatgccg atattttcca
    45721 gggtgtggta agccagcagg cgctggatgt tgtgttccat caacgcatac agaccaccga
    45781 cgaacgcggt gatcatgccg agcaccagca gcgcgatccc ccaccacagc ggtgcattac
    45841 cgcccagcag tgacagggtt aacatgccca gcaggccaat tttcatgact accgtagaaa
    45901 acaacgcggc agctggcgca gaggcgttag catgtgcctg cggcacccag ccgtgcagcg
    45961 gaataatccc agccagcagg ccaaagccaa tcacaccgag cagccagata tcggaaccga
    46021 gcggcagctg ttgcatacgc atatccagca ggcgcagatc cagcgtgcca taacgctgcc
    46081 acagcagcca gcaagcaatc accagcagca gagtgccaag acgccccagc gcaaaccaca
    46141 gtttgccctc tttgctgttg ctggtgagga acaccgcaca caaggccatg acttcggcca
    46201 ttaccacgaa catgccgagg ttgctggcga tgacggcgca gacggcggca gccatcaaca
    46261 tattgatctg caagccgttg cacttcacct gcgcgtggcg atgccagtca atgttataga
    46321 ggctgacaaa cagaccgcaa aggccgaggg taatcagcca aatcgcgtta agcggggaga
    46381 tttgcacatc gtagcttacc agcgacagcg caccgctcac gccaaccgcg ccagtcagta
    46441 cagtgaagcc cgcggctgcc gtatacagac taccaaccgc gccgccaatt ccggctatcc
    46501 agccacttag cgctttttga aaagagaaat gccagaacag cggcggcgac aaaccatgcc
    46561 acgccgctat tgatcaggga aattgcgctc atttagcctc tccactttga gcctgctgaa
    46621 acaaggtgag atcgccaaag tccgtgttaa aggtcagctc acgcttacgt ttgctgacgc
    46681 gggcgatatc ggtgttatcc accagatgca gggctttagt cgggcacatc cgcacacagg
    46741 ccggaccttg ttcatcaaag ctacaaaggt cacatttgac ggcgatcgcg cgaatacctg
    46801 gcacccagtc aagcaatgtg ctgacacgcg ccggagcagg cggtgccggt ggcgctttcg
    46861 gggtattggc gtttgccgga atatccagcg gacggctgcc ggaaaattca attgcgccaa
    46921 acgggcaggc gatgccgcac agtttgcagc ttacgcacag actttcattc aactgcacgg
    46981 ccccatcaac gcgggtgatg gcgttaaccg ggcagaccac cgcgcagggt gcatcttcac
    47041 agcggtgaca gagctgcggc gcagattctt tttcattgag catcactctc aggcgcggca
    47101 ttgattgcag gccgtgctgg cgatgcgtct ctgaacaggc ggcctcacag gtgtggcagc
    47161 cgatacagag cgtggagtcg gcaattacaa aacgattcac caggcattcc tcaggtgatt
    47221 gtcatttttg acgaaaacat gccgatgaaa tgtcattttc gacactcatc gacacgccca
    47281 tccccaaaca ggcgtaacgc ctgcaaaacg ggcaaagcct cagctcatgc tgccgggctt
    47341 tgtcccttta ccagttggct taaattcacc ggcacattgt tttcaatggc ggtgtataag
    47401 ctgtcgtaaa cgccagcgat tttttcgaga tagtgttcca gaacctgttc gcgatcgcgg
    47461 atgttgacct tgccatcaat catgccgtca atgctcagtt gcgcctgcgc aatccgctgt
    47521 ttgtcttcgc catcttttgt ttcgacgtaa tactcgatgg tgtggctgtt aaagccatcc
    47581 gccagggtga cgtaaatacg aaaatgttca aaaaggacga aacagagttc tttgtccggc
    47641 gcgcagctca tggcgtgata caggcgcgct ttcgataacg tgatcccctg aaccagcgaa
    47701 ttgcagtaga tgtgccactg gtcctgtagg cgacgatgcc gctgtgcgat gtaatcggct
    47761 ttctcgctta tttcccaaat agtcattgtc aggttactcg tttaacagag atgccagctt
    47821 taagcatttt ttgtgccaac ttttaattta ttgttattaa agagattttt aagctaaaga
    47881 tgaatttcgt cgccgtgtcg acgtgtcatt tcgacatcat cgacattatt caccgcaggg
    47941 ataatcaaca ctggcgcaat tattgcttgt agctggcaat agttaatggg aggcgatatg
    48001 cacgaaataa ccctctgcca acgggcactg gaattgatcg aacagcaggc cgcaaaacac
    48061 ggcgcaaaac gcgtaactgg ggtctggctc aaaattggcg cattttcttg tgtcgaaacc
    48121 agctctcttg ccttttgttt tgatctggtt tgccgcggca gcgtggcgga aggttgtaaa
    48181 ctgcacctcg aagaacaaga ggccgaatgc tggtgtgaaa catgccaaca gtatgtgacg
    48241 ctactgaccc agcgcgtccg ccgctgtcca cagtgtcatg gtgacatgct acagattgtg
    48301 gcagacgacg gtttacagat tcggcggata gaaatagacc aggagtgagc gatgtgtaca
    48361 acatgcggtt gcggtgaagg caacctgtat atcgagggtg atgaacataa ccctcattcc
    48421 gcgtttcgta gcgcgccatt tgccccggcg gcacgcccgc agatgaaaat caccggcatt
    48481 aaagcgcctg aatttacccc cagccagact gaagaaggcg acctgcatta cggtcatggc
    48541 gaagcgggca ctcacacacc gggcatgagc cagcgtcgga tgctggaagt cgaaattgac
    48601 gtgctggaca aaaacaaccg tctggctgaa cgcaaccgcg cgcgctttgc tgcccgcaag
    48661 caactggtgc tcaacctggt ttccagccct ggttccggta aaaccaccct gctgacggaa
    48721 accttaatgc gcctgaaaga cagcgttccg tgcgcggtta ttgaaggcga ccagcaaacc
    48781 gtgaacgatg ccgcacgcat tcgcgctacc ggtacaccag cgattcaggt gaacaccggt
    48841 aaaggctgcc atcttgacgc acagatgatt gccgacgccg caccgcgtct gccactggac
    48901 gataacggta ttctgtttat cgaaaacgtt ggcaatctcg tatgcccggc cagcttcgat
    48961 ctcggtgaaa aacacaaagt agcggtgctt tccgttaccg aaggtgaaga caaaccgctg
    49021 aaatatccgc atatgtttgc cgccgcctcg ctgatgctgc tcaacaaagt tgacctgttg
    49081 ccgtatctca actttgacgt tgagaagtgc atcgcctgcg cccgcgaagt caatccagaa
    49141 attgaaatca tccttatttc cgccaccagc ggcgaaggga tggaccagtg gctgaactgg
    49201 ctggagacac agcgatgtgc ataggcgttc ccggccagat ccgcaccatt gacggcaacc
    49261 aggcgaaagt cgacgtctgc ggcattcagc gcgatgtcga tttaacgtta gtcggcagct
    49321 gcgatgaaaa cggtcagccg cgcgtgggcc agtgggtact ggttcacgtt ggctttgcca
    49381 tgagcgtaat taatgaagcc gaagcacgcg acactctcga cgccttacaa aacatgtttg
    49441 acgttgagcc ggatgtcggc gcgctgttgt atggcgagga aaaataatgc gttttgttga
    49501 tgaatatcgc gcgccggaac aggtgatgca gttaattgag catctgcgcg aacgtgcttc
    49561 acatctctct tacaccgccg aacgccctct gcggattatg gaagtgtgtg gcggtcatac
    49621 ccacgccatt tttaaattcg gcctcgacca gttactgcct gaaaacgttg agtttatcca
    49681 cggtccgggt tgcccggtgt gcgtactgcc gatgggtaga atcgacacct gcgtggagat
    49741 tgccagccat ccggaagtca tcttctgtac ctttggcgac gccatgcgcg taccagggaa
    49801 acagggatcg ctgttgcagg caaaagcacg cggtgccgat gtgcgcatcg tttactcgcc
    49861 gatggatgcg ttgaaactgg cgcaggagaa tccaacccgc aaagtggtgt tcttcggctt
    49921 aggttttgaa accaccatgc cgaccaccgc catcactctg caacaggcga aagcgcgtga
    49981 tgtgcagaat ttttacttct tctgccagca cattacgctt atcccgacgc tgcgcagttt
    50041 gctggaacag ccggataacg gtatcgacgc gttcctcgcg ccgggccacg ttagtatggt
    50101 tatcggcact gatgcctata attttatcgc cagcgatttt catcgtccgc tggtggtcgc
    50161 tggtttcgaa ccccttgatc tactgcaagg cgtggtcatg ctggtggagc agaaaatagc
    50221 ggcccacagc aaggtagaga atcagtatcg tcgggtggta ccggatgccg gtaacctgct
    50281 ggcgcaacag gcgattgccg atgtgttctg tgtcaacggc gacagcgaat ggcgcggctt
    50341 aggcgtgatt gaatcttctg gcgtgcacct gacgccggat tatcaacgat tcgatgccga
    50401 agcacatttc cgcccggcac cgcagcaggt ctgcgatgac ccgcgcgcgc gttgtggtga
    50461 ggtattaacg ggcaaatgta agccgcatca atgcccgctg tttggtaaca cctgtaatcc
    50521 tcaaaccgca tttggtgcac tgatggtctc ctccgaagga gcgtgcgccg cgtggtatca
    50581 gtatcgtcag caggagagtg aagcgtgaat aatatccaac tcgcccacgg cagcggcggc
    50641 caggcgatgc agcaattaat caacagcctg tttatggaag cctttgccaa cccgtggctg
    50701 gcagagcagg aagatcaggc acgtcttgat ctggcgcagc tggtagcgga aggcgaccgt
    50761 ctggcgttct ccaccgacag ttacgtcatt gacccgctgt tctttcctgg cggtaatatc
    50821 ggcaagctgg cgatttgcgg caccgcgaat gacgttgcgg gcagtggcgc tattccgcgc
    50881 tatctctcct gtggctttat cctcgaagaa ggattgccga tggagacact gaaagccgta
    50941 gtgaccagca tggcagaaac cgcccgcacg gcaggcattg ccatcgttac tggcgatact
    51001 aaagtggtgc agcgcggcgc ggcagataaa ctgtttatca acaccgctgg catgggcgca
    51061 attccggcga atattcactg gggcgcacag acgctaaccg caggcgatgt attgctggtt
    51121 agcggtacac tcggcgacca cggggcgact atccttaacc tgcgtgagca gctggggctg
    51181 gatggcgaac tggtcagcga ctgcgcggtg ctgacgccgc ttattcagac gctgcgtgac
    51241 attcccggcg tgaaagcgct gcgtgatgcc acccgtggtg gtgtaaacgc ggtggttcat
    51301 gagttcgcgg cagcctgcgg ttgtggtatt gaaatttctg aatcagcgct gccggttaaa
    51361 cctgccgtgc gcggcgtttg cgaattgctg ggactggacg ccctgaactt tgccaacgaa
    51421 ggcaaactgg tgatcgccgt tgaacgcaac gcggcagagc aagtgctggc agcgttacat
    51481 tcccatccac tggggaaaga cgcggcgctg attggtgaag tggtggaacg taaaggtgtt
    51541 cgtcttgccg gtctgtatgg cgtgaaacga accctcgatt taccacacgc cgaaccgctt
    51601 ccgcgtatat gctaataaaa ttctaaatct cctatagtta gtcaatgacc ttttgcaccg
    51661 ctttgcggtg ctttcctgga agaacaaaat gtcatataca ccgatgagtg atctcggaca
    51721 gcaagggttg ttcgacatca ctcggacact attgcagcag cccgatctgg cctcgctgtg
    51781 tgaggctctt tcgcaactgg taaagcgttc tgcgctcgcc gacaacgcgg ctattgtgtt
    51841 gtggcaagcg cagactcaac gtgcgtctta ttacgcgtcg cgtgaaaaag acacccccat
    51901 taaatatgaa gacgaaactg ttctggcaca cggtccggta cgcagcattt tgtcgcgccc
    51961 tgatacgctg cattgcagtt acgaagaatt ttgtgaaacc tggccgcagc tggccgcagg
    52021 tgggctatac cccaaatttg gtcactattg cctgatgcca ctggcggcgg gagggcatat
    52081 ttttggtggc tgtgaattta ttcgttatga cgagcgtccc tggagcgaaa aagagttcaa
    52141 tcgtctgcaa acatttacgc agatcgtttc tgtcgtcacc gaacaaatcc agagccgcgt
    52201 cgtcaacaat gtcgactatg agttgttatg ccgggagcgc gataacttcc gcatcctggt
    52261 tgccattacc aacgcggtgc tttcccgcct ggatatggac gaactggtca gcgaagtcgc
    52321 caaagaaatc cattactatt tcgacattga cgatatcagc attgtcttac gcagccaccg
    52381 taaaaacaaa ctcaacatct actccactca ctatcttgat aaacaacatc ccgcccacga
    52441 acagagcgaa gtcgatgaag ccggaaccct caccgaacgc gtgttcaaaa gtaaagagat
    52501 gctgctgatc aatctctacg agcgggacga tttagccccc tatgaacgca tgttgttcga
    52561 cacctggggc aaccagattc aaaccttgtg cctgttaccg ctgatgtctg gcgacaccat
    52621 gctgggcgtg ctgaaactgg cgcaatgcga agagaaagtg tttaccacta ccaatctgaa
    52681 tttgctgcgc cagattgccg aacgtgtggc aatcgctgtc gataacgccc tcgcctatca
    52741 ggaaatccat cgtctgaaag aacggctggt tgatgaaaac ctcgccctga ccgagcagct
    52801 caacaatgtt gatagtgaat ttggcgagat tattggccgc agcgaagcca tgtacagcgt
    52861 gcttaaacaa gttgaaatgg tggcgcaaag tgacagtacc gtgctgatcc tcggtgaaac
    52921 tggctcgggt aaagagctga ttgcccgtgc gatccataat ctcagtgggc gtaataatcg
    52981 ccgcatggtc aaaatgaact gcgcggcgat gcctgccgga ttgctggaaa gcgatctgtt
    53041 tggtcatgag cgtggggctt ttaccggtgc cagcgcccag cgcattggtc gttttgaact
    53101 ggcggataaa agctctctgt tcctcgacga agtgggcgat atgccactgg agttacagcc
    53161 gaagttgctg cgtgtattgc aggaacagga gtttgaacgt ctcggcagca acaaaatcat
    53221 tcagacggac gtacgtttaa ttgccgcgac taaccgcgat ctgaaaaaaa tggtcgccga
    53281 ccgagagttc cgtagcgatc tctattaccg cctgaacgta ttcccgattc acctgccgcc
    53341 actacgcgag cgtccggaag atattccgct gctggcgaaa gcctttacct tcaaaattgc
    53401 ccgtcgtctg gggcgcaata tcgacagcat tcctgccgag acgctgcgca ccttgagcaa
    53461 catggagtgg ccgggtaacg tacgcgaact ggaaaacgtc attgagcgcg cagtattgct
    53521 aacacgcggc aacgtgctgc agctgtcatt gccagatatt gctttaccgg agcctgaaac
    53581 gccgcctgcc gcaacggttg tcgcccagga gggcgaagat gaatatcagt tgattgtgcg
    53641 cgtgctgaaa gaaaccaaca gcgtggttgc cgggcctaaa ggcgctgcgc aacgtctggg
    53701 gctgaaacgc acgaccctgc tgtcacggat gaagcggctg ggaattgata aatcggcatt
    53761 gatttaactg caaattgccg gacagatctg cctgtccggc atactattca cgaggttttt
    53821 tcggacgata tttttccggc agttctggca ccggacgctt gtcatcgatg agatgacgca
    53881 cggttaagat cggatgacgc cacagcattc tcggcccggc ccaacgcata atctgtttca
    53941 tctcttcacg ctttgcaggc tggtaacagt gcaccggaca ctgcttacag gctggtttct
    54001 cttcgccgaa cacacattta tccagccgct tttgcgcgta aacaaacaac gcctcgtaat
    54061 gctccggctc cgctgacgcc tgcgggcatt tcgcttgata aagatcgatc atttttttaa
    54121 tcgtcagttt ttcacgagag atacgcttgc cggacatgct gcctccacct cattaagatg
    54181 catttatatt acatcttaat
//
LOCUS       DAEEGA010000019        53213 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEEGA010000019 DAEEGA010000000
VERSION     DAEEGA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53213
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.019"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(11..322)
                     /locus_tag="KLH46_001182"
     CDS             complement(11..322)
                     /locus_tag="KLH46_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBE0377940.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="KLH46_001183"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KLH46_001183"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="KLH46_001184"
     CDS             701..1054
                     /locus_tag="KLH46_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBE0377941.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="KLH46_001185"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="KLH46_001185"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBE0377942.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="KLH46_001186"
     CDS             complement(2810..3280)
                     /locus_tag="KLH46_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBE0377943.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="KLH46_001187"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="KLH46_001187"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBE0377944.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="KLH46_001188"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="KLH46_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBE0377945.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="KLH46_001189"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="KLH46_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBE0377946.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="KLH46_001190"
     CDS             complement(5283..8432)
                     /locus_tag="KLH46_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBE0377947.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRNNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="KLH46_001191"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="KLH46_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBE0377948.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="KLH46_001192"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="KLH46_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBE0377949.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="KLH46_001193"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="KLH46_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBE0377950.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="KLH46_001194"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="KLH46_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBE0377951.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="KLH46_001195"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="KLH46_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBE0377952.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHRELEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="KLH46_001196"
     CDS             14911..15288
                     /locus_tag="KLH46_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBE0377953.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="KLH46_001197"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="KLH46_001197"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBE0377954.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="KLH46_001198"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="KLH46_001198"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBE0377955.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="KLH46_001198"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="KLH46_001199"
     CDS             18099..18428
                     /locus_tag="KLH46_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBE0377956.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="KLH46_001200"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="KLH46_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBE0377957.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="KLH46_001201"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="KLH46_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBE0377958.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="KLH46_001202"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="KLH46_001202"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBE0377959.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="KLH46_001203"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="KLH46_001203"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBE0377960.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="KLH46_001204"
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="KLH46_001204"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBE0377961.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="KLH46_001205"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="KLH46_001205"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBE0377962.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="KLH46_001206"
     CDS             complement(25481..27157)
                     /locus_tag="KLH46_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBE0377963.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="KLH46_001207"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="KLH46_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBE0377964.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="KLH46_001208"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="KLH46_001208"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBE0377965.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="KLH46_001209"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="KLH46_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBE0377966.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="KLH46_001210"
     CDS             complement(31205..31999)
                     /locus_tag="KLH46_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBE0377967.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="KLH46_001211"
     CDS             32137..32478
                     /locus_tag="KLH46_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBE0377968.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="KLH46_001212"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="KLH46_001212"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBE0377969.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="KLH46_001213"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="KLH46_001213"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBE0377970.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="KLH46_001214"
     CDS             36394..37686
                     /locus_tag="KLH46_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE0377971.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38245
                     /gene="cueR"
                     /locus_tag="KLH46_001215"
     CDS             37811..38245
                     /gene="cueR"
                     /locus_tag="KLH46_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBE0377972.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAGGLRRDGSDM"
     gene            complement(38219..38677)
                     /locus_tag="KLH46_001216"
     CDS             complement(38219..38677)
                     /locus_tag="KLH46_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBE0377973.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="KLH46_001217"
     CDS             complement(38674..39591)
                     /locus_tag="KLH46_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBE0377974.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="KLH46_001218"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="KLH46_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBE0377975.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="KLH46_001219"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="KLH46_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBE0377976.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="KLH46_001220"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="KLH46_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBE0377977.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLQDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="KLH46_001221"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="KLH46_001221"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBE0377978.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="KLH46_001222"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="KLH46_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="KLH46_001223"
     CDS             43550..44236
                     /locus_tag="KLH46_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0377979.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /locus_tag="KLH46_001224"
                     /pseudo
     CDS             44233..46648
                     /locus_tag="KLH46_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="KLH46_001225"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="KLH46_001225"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBE0377980.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="KLH46_001226"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="KLH46_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBE0377981.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="KLH46_001227"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="KLH46_001227"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBE0377982.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="KLH46_001228"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="KLH46_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBE0377983.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="KLH46_001229"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="KLH46_001229"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBE0377984.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53213
                     /locus_tag="KLH46_001230"
     CDS             52938..>53213
                     /locus_tag="KLH46_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBE0377985.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIR"
BASE COUNT        12830 a        13691 c        14021 g        12671 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgtggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctggacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattattgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccaattc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cttggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tagaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tacggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgaccat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggggatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct ctggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtcttgcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggagttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcgatctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cagtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggaggc gcgttcatcg ttatccatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtggggtgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgcagctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcc
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ctcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gagttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc
    51121 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg ggggtttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt cgt
//
LOCUS       DAEEGA010000020        52075 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEEGA010000020 DAEEGA010000000
VERSION     DAEEGA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52075
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.020"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(262..2541)
                     /gene="maeB"
                     /locus_tag="KLH46_001231"
     CDS             complement(262..2541)
                     /gene="maeB"
                     /locus_tag="KLH46_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBE0377986.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2830..3780
                     /gene="tal"
                     /locus_tag="KLH46_001232"
     CDS             2830..3780
                     /gene="tal"
                     /locus_tag="KLH46_001232"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBE0377987.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3800..5803
                     /gene="tkt"
                     /locus_tag="KLH46_001233"
     CDS             3800..5803
                     /gene="tkt"
                     /locus_tag="KLH46_001233"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBE0377988.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5906..6949)
                     /locus_tag="KLH46_001234"
     CDS             complement(5906..6949)
                     /locus_tag="KLH46_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBE0377989.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="KLH46_001235"
     CDS             complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="KLH46_001235"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBE0377990.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="KLH46_001236"
     CDS             complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="KLH46_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBE0377991.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVLIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9903..11620
                     /gene="narQ"
                     /locus_tag="KLH46_001237"
                     /pseudo
     CDS             9903..11620
                     /gene="narQ"
                     /locus_tag="KLH46_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11822..14935
                     /gene="acrD"
                     /locus_tag="KLH46_001238"
     CDS             11822..14935
                     /gene="acrD"
                     /locus_tag="KLH46_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBE0377992.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="KLH46_001239"
     CDS             complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="KLH46_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBE0377993.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            15474..15830
                     /locus_tag="KLH46_001240"
     CDS             15474..15830
                     /locus_tag="KLH46_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBE0377994.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            15834..16961
                     /gene="dapE"
                     /locus_tag="KLH46_001241"
     CDS             15834..16961
                     /gene="dapE"
                     /locus_tag="KLH46_001241"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBE0377995.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            16989..17189
                     /locus_tag="KLH46_001242"
     CDS             16989..17189
                     /locus_tag="KLH46_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBE0377996.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="KLH46_001243"
     CDS             complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="KLH46_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBE0377997.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="KLH46_001244"
     CDS             complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="KLH46_001244"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBE0377998.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(20101..20964)
                     /locus_tag="KLH46_001245"
     CDS             complement(20101..20964)
                     /locus_tag="KLH46_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBE0377999.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(21132..21845)
                     /gene="purC"
                     /locus_tag="KLH46_001246"
     CDS             complement(21132..21845)
                     /gene="purC"
                     /locus_tag="KLH46_001246"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBE0378000.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="KLH46_001247"
     CDS             complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="KLH46_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBE0378001.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="KLH46_001248"
     CDS             complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="KLH46_001248"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBE0378002.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            24133..24705
                     /locus_tag="KLH46_001249"
     CDS             24133..24705
                     /locus_tag="KLH46_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBE0378003.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            24705..25175
                     /gene="bcp"
                     /locus_tag="KLH46_001250"
     CDS             24705..25175
                     /gene="bcp"
                     /locus_tag="KLH46_001250"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBE0378004.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            25428..26045
                     /gene="hyfA"
                     /locus_tag="KLH46_001251"
     CDS             25428..26045
                     /gene="hyfA"
                     /locus_tag="KLH46_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBE0378005.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            26045..28062
                     /gene="hyfB"
                     /locus_tag="KLH46_001252"
                     /pseudo
     CDS             26045..28062
                     /gene="hyfB"
                     /locus_tag="KLH46_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            28073..29020
                     /gene="hyfC"
                     /locus_tag="KLH46_001253"
     CDS             28073..29020
                     /gene="hyfC"
                     /locus_tag="KLH46_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBE0378006.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            29037..30476
                     /gene="hyfD"
                     /locus_tag="KLH46_001254"
     CDS             29037..30476
                     /gene="hyfD"
                     /locus_tag="KLH46_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HBE0378007.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGQVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            30488..31138
                     /gene="hyfE"
                     /locus_tag="KLH46_001255"
     CDS             30488..31138
                     /gene="hyfE"
                     /locus_tag="KLH46_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HBE0378008.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            31143..32729
                     /gene="hyfF"
                     /locus_tag="KLH46_001256"
     CDS             31143..32729
                     /gene="hyfF"
                     /locus_tag="KLH46_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBE0378009.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            32719..34433
                     /gene="hyfG"
                     /locus_tag="KLH46_001257"
                     /pseudo
     CDS             32719..34433
                     /gene="hyfG"
                     /locus_tag="KLH46_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            34443..34988
                     /gene="hyfH"
                     /locus_tag="KLH46_001258"
     CDS             34443..34988
                     /gene="hyfH"
                     /locus_tag="KLH46_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBE0378010.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            34985..35743
                     /gene="hyfI"
                     /locus_tag="KLH46_001259"
     CDS             34985..35743
                     /gene="hyfI"
                     /locus_tag="KLH46_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HBE0378011.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            35736..36149
                     /gene="hyfJ"
                     /locus_tag="KLH46_001260"
     CDS             35736..36149
                     /gene="hyfJ"
                     /locus_tag="KLH46_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBE0378012.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            36179..38191
                     /gene="hyfR"
                     /locus_tag="KLH46_001261"
     CDS             36179..38191
                     /gene="hyfR"
                     /locus_tag="KLH46_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HBE0378013.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLSSVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            38213..39061
                     /locus_tag="KLH46_001262"
                     /pseudo
     CDS             38213..39061
                     /locus_tag="KLH46_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(39099..40160)
                     /locus_tag="KLH46_001263"
     CDS             complement(39099..40160)
                     /locus_tag="KLH46_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBE0378014.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            40373..41836
                     /gene="bepA"
                     /locus_tag="KLH46_001264"
     CDS             40373..41836
                     /gene="bepA"
                     /locus_tag="KLH46_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBE0378015.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            41857..42216
                     /gene="arsC"
                     /locus_tag="KLH46_001265"
     CDS             41857..42216
                     /gene="arsC"
                     /locus_tag="KLH46_001265"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBE0378016.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(42349..43095)
                     /gene="hda"
                     /locus_tag="KLH46_001266"
     CDS             complement(42349..43095)
                     /gene="hda"
                     /locus_tag="KLH46_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBE0378017.1"
                     /translation="MVNFSRFCEILVEVSLNTQAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="KLH46_001267"
     CDS             complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="KLH46_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBE0378018.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(44520..45146)
                     /gene="upp"
                     /locus_tag="KLH46_001268"
     CDS             complement(44520..45146)
                     /gene="upp"
                     /locus_tag="KLH46_001268"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBE0378019.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            45471..46508
                     /gene="purM"
                     /locus_tag="KLH46_001269"
     CDS             45471..46508
                     /gene="purM"
                     /locus_tag="KLH46_001269"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBE0378020.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            46508..47146
                     /gene="purN"
                     /locus_tag="KLH46_001270"
     CDS             46508..47146
                     /gene="purN"
                     /locus_tag="KLH46_001270"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBE0378021.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            47318..49384
                     /gene="ppk1"
                     /locus_tag="KLH46_001271"
     CDS             47318..49384
                     /gene="ppk1"
                     /locus_tag="KLH46_001271"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBE0378022.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            49389..50930
                     /gene="ppx"
                     /locus_tag="KLH46_001272"
     CDS             49389..50930
                     /gene="ppx"
                     /locus_tag="KLH46_001272"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBE0378023.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(50969..>52075)
                     /gene="pdeF"
                     /locus_tag="KLH46_001273"
     CDS             complement(50969..>52075)
                     /gene="pdeF"
                     /locus_tag="KLH46_001273"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBE0378024.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        12157 a        13738 c        13520 g        12660 t
ORIGIN      
        1 cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg
       61 tagatcaata agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat
      121 gaatataaat aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac
      181 tcagtaataa gctttgctaa tatatttcag gaaaaccgct taccctgaat attcagggta
      241 agcgtgagag ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc
      301 caccatattg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc
      361 cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt
      421 gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa
      481 agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat
      541 ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat
      601 tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg
      661 cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc
      721 ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata aaggtgttac cactcggcag
      781 cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt
      841 caccacgcta aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc
      901 cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc
      961 ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt
     1021 aaagcgcgga tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa
     1081 gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc
     1141 cagtcccagc gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg
     1201 cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt
     1261 ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg
     1321 cgtcgccacg cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa
     1381 gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc
     1441 atacgctgaa gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac
     1501 cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg cgccacggaa
     1561 gatgaacggg aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca
     1621 gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt
     1681 cgccagcgcc aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac
     1741 tttcgggccg gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg
     1801 tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacggcc
     1861 ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc
     1921 taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac
     1981 gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat
     2041 tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg
     2101 ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg
     2161 ttcgagcgcg gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc
     2221 aaatacatca atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt
     2281 gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc
     2341 aaccaggtta ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt cgatttcaag
     2401 acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg
     2461 cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact
     2521 ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt
     2581 gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga
     2641 ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat cctataacac
     2701 tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt
     2761 tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga
     2821 gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc
     2881 tgcgatattg agtccattcg ccattatcat ccccaggatg ccaccactaa tccttcgctg
     2941 ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg
     3001 ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg
     3061 gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat
     3121 gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg
     3181 tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa
     3241 ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg
     3301 ttttctttta cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt
     3361 gtagggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa
     3421 gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat
     3481 gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc
     3541 tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca
     3601 gtggtacgta aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa
     3661 gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc
     3721 attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa
     3781 accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag
     3841 tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga
     3901 tattgccgaa gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta
     3961 tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct
     4021 acatctgacc ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc
     4081 gaaaacccca ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc
     4141 gcttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc
     4201 gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga
     4261 cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact
     4321 aggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg
     4381 ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga
     4441 aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa
     4501 agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc
     4561 aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca
     4621 aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga
     4681 tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa
     4741 aaaggctcat ccgcaactgg cagaagagtt tccccgacgg atgagcggtg gtttaccgaa
     4801 ggactgggag aaaacgactc agaaatatat caatgagtta caggcaaatc cggcgaaaat
     4861 cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt
     4921 gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc
     4981 gctgaaggaa gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac
     5041 cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgttcct
     5101 gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca
     5161 gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc
     5221 tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga
     5281 tcagatggaa gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc
     5341 actgatcctc tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga
     5401 gattgctcgt ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat
     5461 tgccaccggt tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg
     5521 tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga
     5581 atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat
     5641 tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta
     5701 cggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat
     5761 tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc
     5821 tgtatgttcc cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt
     5881 tttagggccg catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg
     5941 gcccatgcca gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac
     6001 cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg
     6061 tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct
     6121 cctggccgtt gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac
     6181 gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca
     6241 tcagtgcttt atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg
     6301 agcagcgcag accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga
     6361 gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg
     6421 gtacgctgag aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct
     6481 tttgctgagc atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat
     6541 tgccgtcgcg taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg
     6601 ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg
     6661 ccaacggctc accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg
     6721 acttcaaccc accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc
     6781 tcagggtcat caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat
     6841 tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag
     6901 acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga
     6961 ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca
     7021 atcggatttg tcggattgag ccaataataa acaacgaaat attattgcta tttttcagtc
     7081 cattaaatgt gacatttgca aatagttaag caataacacc gtcttaccgt cacgtatctc
     7141 gccggttttg atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc
     7201 atcttcaaca ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa
     7261 gtggattagc tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc
     7321 gcccacttca tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc
     7381 gttatccagc agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca
     7441 ggtagcgaca cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag
     7501 gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc
     7561 tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt
     7621 gtctttaatg agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta
     7681 tattacgctg tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta
     7741 cccccagcca gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca
     7801 ccagatccgc accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt
     7861 aacggtactg gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt
     7921 gcgactccag ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg
     7981 ctggcatgac aaattcactg ccctcaatca gtacaggtcg ccgacgcccc tgggcatccg
     8041 gctcgccaag acgcgtgcgc aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct
     8101 caagcgccac cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca
     8161 cctctttctt cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac
     8221 tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta
     8281 ccacgacgtt aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca
     8341 tcacctgttt agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt
     8401 cattgggtaa acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt
     8461 attgttccaa aagcaaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt
     8521 gaatccccat cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga
     8581 aagaaggaat gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca
     8641 ccgccacgcc attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga
     8701 taatcgccac ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg
     8761 ccaacgcctg atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat
     8821 cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt
     8881 ccggcagggt gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca
     8941 ccagttcaat ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt
     9001 gctcgccgaa cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca
     9061 gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac
     9121 gcgcgggcgt tgcctgcatt tgttcgactt tactcattac cggcatttct tgtgcagcgg
     9181 tactggcatg ccacggttga tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca
     9241 tgccggagag tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac
     9301 aggcaggacc gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt
     9361 ttcctgccgc gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc
     9421 aggatttaca gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga
     9481 ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg
     9541 cactacgttg ctgctgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca
     9601 ggacatgttg ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca
     9661 gacattgctg actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta
     9721 aaaaccttat tctttatatg agtgttgtta ttacccgact tacaggggaa ccggcaatgt
     9781 tcatgtgccc agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat
     9841 cactgactaa attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc
     9901 gtgtgattgt taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc
     9961 tgctgtcgat tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg
    10021 ctgaggctat caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact
    10081 tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt
    10141 caccggtatt aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg
    10201 cgcatctgaa tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt
    10261 ggtatcaggc caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac
    10321 agcactacgc tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg
    10381 gtattttcac gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc
    10441 tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc
    10501 tggataccag tctgccgaat gagcttggtc tgcttgcaaa aacctttaac caaatgtcga
    10561 gcgagctgca taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc
    10621 tccacgagac caagcgtcgt ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa
    10681 caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga
    10741 agcagctgaa tatctggagc tgaacgttgg ggataactgg cggattagcg aagggaagcc
    10801 aaaccctgag ctgccaatgc agattttacc ggtgacaatg caagagacgg tttacggcga
    10861 actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc
    10921 gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt
    10981 gatggaagaa cgtgcgacca tcgcccgcga attgcacgac tcgctggctc aggtgctttc
    11041 ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc
    11101 acaaagtatc atggccgatt ttttccaggc gttgaatgat gcttatcggc agttacgcga
    11161 gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga
    11221 aatgctggat acgttacaaa atcaaaccag cgctaaactg accctcgact gccgtctgcc
    11281 aaccctggca ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt
    11341 gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc
    11401 ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga
    11461 acccgaaggt cattatggac tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac
    11521 gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctccgc
    11581 agagggtgag gggaagggga acacgccggc tgaggtgtaa ataattaccc tcacaccggc
    11641 cctctccctt taaaggcgag agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt
    11701 cagcaatctc atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt
    11761 gccgctacag tgaagcaagt caagcctaca acaatacgca gaaacacgag gtcctctttt
    11821 aatggcgaat ttctttattg atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg
    11881 tctgacaggt accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc
    11941 accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac
    12001 cgtgacccag gttatcgagc aaaatatgac cggcctcgat aatctgatgt atatgtcatc
    12061 tcagagcagt ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc
    12121 ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca
    12181 ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc gataccaaca ttctgaccat
    12241 tgccttcgtc tctaccgatg gctcgatgga taaacaggat attgctgatt atgttgccag
    12301 taatattcag gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc
    12361 gcaatattcc atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc
    12421 taaagatgtc actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg
    12481 tggtacacct tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct
    12541 gcaaacacca gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaggt
    12601 aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg
    12661 cttcaatggt aagccagcct ccgggttggg ggtaaaactg gcctccagcg caaacgaaat
    12721 ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg
    12781 actggaatac aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt
    12841 ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca
    12901 aaacttccgc gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt
    12961 ctccgtactt tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct
    13021 ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat
    13081 gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg
    13141 ggcactggtc gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg
    13201 cggcaccacc ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct
    13261 gtcagtactg gtagcgatgc tcctcactcc ggctctgtgt gccacgctac ttaagccact
    13321 gaaaaaaggt gagcatcacg ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa
    13381 ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg
    13441 gattgtgatt tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac
    13501 gtcgttctta ccgctggaag accggggcat gtttactacc tcggtacaat tgcccagcgg
    13561 ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca
    13621 tgaaaaagac aacatcatgt cggtgtttgc caccgttggt tctggccctg ggggtaacgg
    13681 gcaaaacgtg gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac
    13741 cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc
    13801 tcgcgttatc gccagcagcc caccagcaat tagcggtctt ggtagttctg caggttttga
    13861 tatggagttg caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaatca
    13921 gttgctggcg ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga
    13981 tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga
    14041 tatcgacgat attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt
    14101 tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct
    14161 gccagatgac atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc
    14221 tgctttcgcg acctcacgct gggaaacagg ctcgccgcgt ctggaacgct ataacggtta
    14281 ttctgcggtt gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga
    14341 tattatggaa tcgttagtga agcagctgcc aaacggcttt ggtctggagt ggacggcgat
    14401 gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct
    14461 ggtggtattc ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt tctcggtaat
    14521 gctggtcgtg ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga
    14581 aaacgacgtt tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc
    14641 catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc
    14701 gacgctccac gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat
    14761 cttcggcgta ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt
    14821 gggtactggc gtaatgggcg ggatgatttc agctactatt ctggctattt acttcgtgcc
    14881 gctgttcttt gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat
    14941 aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag
    15001 cgggttcttg ctgtttatac tttgtctcag gaattactta cgaagcataa cttcgataaa
    15061 gtctttccag ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat
    15121 tctacggaaa caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc
    15181 cgggggacaa accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac
    15241 agcaacattt cgagatattc atacggcatc taatactgat ttaattctgg ttaaaataca
    15301 gacagataac aagatgaata ttcttaatgt ttacgttaaa aatgtttaat attatttaat
    15361 agttgttaat ttgaatactt cgataatgtt atattttctg ataatcattt gcaggcaaaa
    15421 tgttttcacc cttaaatgag tatttattct cataaatcga aaaaggattc attatggtta
    15481 cactttacgg catcaaaaat tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca
    15541 ataacatcga ctatcgtttt catgattacc gcgtcgatgg gctggacagc gaattattga
    15601 acggttttat caacgaatta ggctgggaag cgttactcaa cacccgtggt acaacctggc
    15661 gtaaactgga cgaaactacc cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa
    15721 tgactgaaat gcctgcaatt atcaaacgtc cattgctctg cgcgcctggt aagcctatgc
    15781 tgctgggttt cagtgattcc agttatcagc aatttttcca tgaggtgtag tctatgtcgt
    15841 gcccggttat tgagctgaca caacagctta ttcgccgccc ttccctgagt cctgatgacg
    15901 caggatgtca ggctttgttg attgaacgtt tgcaggcgat cggttttacc gttgaacgca
    15961 tggattttgc cgatacgcag aatttttggg catggcgtgg gcaaggtgaa acgttagcct
    16021 ttgccgggca taccgacgtg gtgccgcctg gcgacgccga tcgttggatc aatccgccgt
    16081 ttgaacccac cattcgtgac ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct
    16141 cgctggcggc gatggtggta gcagcagaac gttttgtcgc acaacatccc aaccatacgg
    16201 ggcgactggc atttctgatc acttctgatg aagaagccag tgcccacaac ggtacggtaa
    16261 aagtcgtcga ggcgttaatg gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac
    16321 cgtcgagtat cgaagtggta ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct
    16381 gcaacctcac cattcatggc gttcaggggc atgttgccta cccacatctg gctgacaatc
    16441 cggtacatcg cgcagcacct ttccttaatg aattagtggc tattgagtgg gatcagggca
    16501 atgaattctt cccggcgacc agtatgcaga ttgccaatat tcaggcggga acgggcagta
    16561 acaatgttat tccgggtgaa ctgtttgtgc agtttaactt ccgcttcagc accgaactga
    16621 ctgatgagat gatcaaagcg caggtacttg ccctgcttga aaaacatcaa ctgcgctata
    16681 cggtggattg gtggctttcc gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg
    16741 cggtcgttaa cgcggttgag cactataatg aaattaaacc gcagctactg accacaggcg
    16801 gaacgtccga cgggcgcttt attgctcgca tgggggcgca ggtggtggaa ctcgggccgg
    16861 tcaatgccac tattcataaa attaatgaat gtgtgaacgc tgccgacctg cagctacttg
    16921 cccgtatgta tcaacgtatc atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg
    16981 aaataagcat ggactggctg gctaaatatt ggtggattct ggtgattgtc tttttggtag
    17041 gcgtcctgct gaacgtgatt aaagatctca agcgcgtcga ccataagaaa tttctcgcca
    17101 acaagccgga gcttcccccg catcgtgatt tcaacgataa gtgggacgat gacgacgact
    17161 ggccgaaaaa ggatcaaccg aagaagtaag ctgagtgctc aaccgtaggc cggataaggc
    17221 gttaacgccg catccggcaa tggtgaacga tccctgatgc gacgctcacg cgtcttatca
    17281 ggccaggtaa tctgtctttt acatcatttc aatcacatca tcatcgccag gcttaccgcc
    17341 acttagcgct tggtcgaaat aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa
    17401 ttgcatactg cgattgtcga tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc
    17461 accaccggca ctgattaacg cttcctgcgc cgcaactgca tgcgccagat cgataaccgg
    17521 atcttcacca ccatgaatca agtgaattgt cgtggctgta gacgcggtct ccggcaggct
    17581 cgcatagcgt ccgttaaagg caatgacgcg cgaagcaagg cccggttcgg ctttaatgct
    17641 ctccagcgcc ataatcgcgc cttgtgagaa accaattagc gccgtggcgt tagcccctac
    17701 tccgctctgt ttctgccagt agcgtaccgt ttcaataaac gtcggcatga tggcatccac
    17761 acgcgcctgg cgattatctt ccgtaatacc ctgcactgaa aaccactgac gccccgccgg
    17821 attaccgctc ggctccgcgc caccgacact caccaccagc gcatcaggaa acagcggtgc
    17881 aaaccagctg cctatttccc ccattgccac cgggttatcc ccgacaccgt ggaaaagcag
    17941 taacaactgt tgtgcaggtt tatccgggct ttgaacaaca aaatggtcat gtttcatggc
    18001 gaactcctta actgatgtca ttaattttac gccgctgact cacaatgacc atgggagaaa
    18061 attgaaggag tcagtgaaaa aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc
    18121 gaacctcatt tagtgcgaac agcgcctgcg cggcctcttc ccgctggcga accagtaaca
    18181 tcttgcggcc tgaaagttta agtgtggtac ataactgcgc gtcactggcg tttttctgta
    18241 aacgcccacg cagcgccggt aatgccagtt cactggtttg tagcagacgc aataagcaac
    18301 ctaacgatgt taatagcgga cgatgagcga aagcaaaacc ggccagttca agccagtcgt
    18361 cgtcagaaag gatggcatcg tttagtggat caacaggaag catttcgcca ttccactgcg
    18421 cgagagcttg cgcatcgcga cgtaaacggt aatgctcacg ttcagccagc tgtttacccg
    18481 catcactcat cggtaacagc gccatcgccg tatagcaacc gctgctggct tcccgatgat
    18541 tacccatccg caccagcaca aaaccgcagc gttgccagaa acgccataac tccccagtgt
    18601 aaccaaaact caccgaaaga tagtcgaggt catgcgtata ttgcaaagct ccagcaataa
    18661 gttgtcgccc tgtgccttcc cgctgacgag ccggatgaac tgctatccgg ctaacccgcc
    18721 ttccgcgcaa tgtcgccgcc agtggattgc tgccgtgcgc cgccagcgac tgggccacca
    18781 gattaccccg cgggcgacga aaacctgccc ataccgcctg actgagttct tgagataatc
    18841 cgccctcatc caccagccac aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa
    18901 aatgttgccc tggtgcatcc atcatccggc gtaaatccag cggcgaggtc cggtagtgcg
    18961 caccagacaa taactgataa acctttaacg gcgtttctgg ctcgcttcgc cataacgttt
    19021 gttcaaatgc ggaaatgacg atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa
    19081 aaaccagtgc ctcactaacc attttttcca gcgggcatcc ctgtgcccag cggatcggct
    19141 gttgcagttc aaaacggtgt aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac
    19201 gtccggtacc ttcgtagccc tgcaccgtag tggttaacaa cgttcgagga aaacgcgata
    19261 ccagttgatg caacaacggc gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg
    19321 cttgctcatc gctggctaac aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa
    19381 attgtgccag tacatccgtt gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa
    19441 tacgagaaat gagttgccct gccaacgccg acttaccgcg cccacgcgca gccgttaccg
    19501 ctgccacgcc cggcggcatg gtcagtagct gctgtaagag ttgctgttgt tctggttgtg
    19561 gtgcgccggt cgcggggtgc cagtcagtac ggggagtaaa atgcgccaac gagaacggct
    19621 ggttttgccg ccagaggata gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga
    19681 caaaatgcgg cgtcgcaata gggtcagggc aatcactcca gcgcagcgag tcggcatcag
    19741 gttggttttc ccactcttcc catacaggga gtaacaaaac cagccagctt cccgctttca
    19801 acgttccgct aagtgctgca aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg
    19861 catgccggaa ctcgcgccca agtaaagttt gtagcgccga gggtgaacag tggttttcag
    19921 catctagctg cggcgaaatc cacagccagt cgccaggtaa ggcatcacgc aacttaagag
    19981 catgatcaaa acaccaaccc tcttccccgc tcaacaccag caagcggcag atcccttcac
    20041 gtttcatttg cgctgttaat gtgtaaagcg cagtcagttc agccatccct gccccggaaa
    20101 ttaaatgctt ttaccaaaag tattgcattg tgccggatcg ccgctgtcga aaccacgttt
    20161 aaaccagctg tagcgttgct gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc
    20221 ctgactttgc tgttgtaaac ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc
    20281 cagatcgcca gtttccagaa cgccttgctg ctgcatacta tgcccccaga caccggcaaa
    20341 acagtcggcc tggagttcca tacgcacaga taagcggttt acttccgcct gcgtcgcgtt
    20401 ttgttgcagt tgacgaactt tcggctcgat gcctaacagt ttctgcacat gatgaccgac
    20461 ttcatgggcg ataacgtacc cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat
    20521 gtcatcatag aaggagagat cgatataaac tgtgccatcc gccgggcaat agaacggccc
    20581 cataatggac tggcccgccc cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt
    20641 cggttgctga taggtcttac ccatcttttc gaactgttgt ccccaggtgt cttccgtggt
    20701 tgccagaatc accgaggtga attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt
    20761 tgattgttgt tgagaaaccg gctgcccggt catcaacccg gttaaatcaa cgccatagta
    20821 gcctgcaacc agcacgacta tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa
    20881 accgggaccg cccattgacg gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact
    20941 ttcacgtcgc ccttgccaac gcatagatac ctcaacaata tattcattat agaaatgatc
    21001 gtaggtggtt cagaggaaga ttaccacagg aaagcgggat gaaatggcgg aggaatgagc
    21061 gttcaatgca tatattccga gagcggttgt cttgccgtgc cagctgcacg gcaagatgat
    21121 gaacagaaaa atcagtccag ctgtacaccc aggcggcggg cgacggcttc ataggcttcg
    21181 atcaggccac cgaggctctg gcggaaacgg tctttgtcca ttttctccag tgtttctttg
    21241 tcccacaggc ggctgccgtc cggggagaac tcatcgccca gtaccacttc acctttgtac
    21301 agaccaaact ccagcttgaa gtcgaccaga atcagaccag catcatcgaa cagttttttc
    21361 agcacgtcgt tcgctttgta ggtcagctct ttcatacgcg ccaggttctc tttgctcacc
    21421 cagccaaagg tttcgcagta agattcgttg accatcggat cgtgcatggc atcgtttttc
    21481 aggaacagat cgaacagcgg cgggttcagc tcaatacctt cttcgattcc aagacgtttc
    21541 accagagagc cagcagcacg gttacgcacg acacactcaa ccggcaccat atccagcttt
    21601 ttcaccagac attcggtatc ggagagcaga cgctccattt gagtcgggat acccgcttca
    21661 gccagtttgc tcataatgaa gtagttgaac ttgttgttca ccataccttt gcgatcaaac
    21721 tgctcaatgc gcgcgccatc ccctgctgac gtatcattgc ggaattcgag caccaacagg
    21781 tccgggtttt ccgtgctgta tacggttttc gctttaccac gatacaactc agcttgcttt
    21841 tgcatcttta tcactcctgg gtgtgaatta acgttttaaa atcttttgct gtctggtgtg
    21901 ccggatgttt tgtcggatgc ggcgtgaacg ccttatccga actacgtccg acaaacaaat
    21961 ttcgtgcgaa ttaccgctaa aatcgcgttt tcctgtcgac gcacacgttt gcgtatcata
    22021 tcagaaaaaa gggccggatg attccagccc tgtattttta cttgctaaac gcagcctgga
    22081 agacagctac cagcgcgtcg ttctgactct gagtcagagt atgacctttc ggatcgatga
    22141 actgtaggct gctgcggtta tctaaatcgc caacctgcag tttatagtca ccggatgcca
    22201 ggcctggatc gctcgcgccc agttcctgcc agtcgctgtc agacagcggc ttataagtta
    22261 cggccatgtt gccctgcgaa cgggtgctgt cggtcacttt catgcccact ttttccagcg
    22321 ccgctggcag acgttgccaa accacattga acggcccgcg tacgaccagc attggtaaac
    22381 cggtgtcatc agctgcactt tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt
    22441 tcgcggcgtc agtggcagat ttatccagac cggcggaaat aacgttcatc atctccgtgc
    22501 tgtaacgctg catggaagcc gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca
    22561 gtttaaccgt aaccgcctgc tgataaccct gcggcttaac agagatttga taacgaccac
    22621 gatactgctc gtcttcgtcc agacggttcc attgtaccca atcggtggtc agtgtctgac
    22681 cagcatcatc acgttgggtg atggtgtagt ttttcgcctg cagcacgcta accacctgcg
    22741 gccacagagt attgccacga ccattttcca ccagcaatga agcggtatcg cccgtgaact
    22801 gggtacgcgc gccagaaacc agtgccaacg gctgggctgg tggacgaatg tccagcgcct
    22861 taccgacagc accactaccg ttggtcaccg ggattgcata atcaccggag gtcaccggca
    22921 aaatcattcc agccggggca tgaagctccg caagcggtgc cgcttccagg taggcttcat
    22981 caccactgac ctgacgctta tagcgtgagt cagaactaca ggcagcgagt aataaaacaa
    23041 gcgaaacacc cgcaaccttt gccaggcgcg acttttgaac agagtaagcc atcaaatctc
    23101 cctaaacttt acagcaaacc ggcatgctta agcgccgctc tgaccgtctc acgaccactg
    23161 tcggtgattg gtgtcattgg caggcgcagc gtatcggtcg ccacaagacc cagttcctta
    23221 catgcccatt tcaccgggat tggattgggt tcgacaaata gtttgttgtg taatggcatc
    23281 agacgctgat taataacgcg tgcctcggca aaatgccctt ctgctgccag tttgcacatc
    23341 tgggccatat cacgcgctgc gacgttagcc gtaacggaaa taactccatg accgcccaat
    23401 tgcatgaagt ccagcgcgct cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc
    23461 agctctttga tctggtttac acgcgttaag ttccctgttg cctctttgat tccgataata
    23521 ttttttactt tcgccagacg gcccaccgtt tccgggagca gatcgcagcc agtacgggac
    23581 ggcacattat acagaatttg cggcaggtca gtatgctcag cgatggcttt gaaatgctga
    23641 tacaaacctt cttgcgacgg acgattgtag taaggggtta ccgtcaggca gccgacgata
    23701 ccactgtcat tgaagcgctg cgtcaggcta atggcttccg cagtagcgtt agcgccggtc
    23761 ccggcaatta ccggaatgcg cccatcagcc agatccagcg tcatcatcac cacatcagca
    23821 tgttcgtcat gatttaaggt agcggactcg ccagtggtgc caacagaaac gatcgccgaa
    23881 gtaccgctgg cgacatgata atcaatcagt tttttcaagc tagcccgaca gacattacct
    23941 ttttcatcca tcggagtaac aatcgcgaca atacttcccg tgaacatggg ccatcctctg
    24001 tgcaaacaag tgtctcaatg gtacgtttgg tatggcatta aaagcaagca gacagaaccg
    24061 ttctgattgt tgtatgcatg atttttttat gctttcctta agaacaactc accccttaaa
    24121 ggaataacca gtttgacact gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc
    24181 gatcgccctg gaattgtgaa caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt
    24241 gaagacagtc gcctggcgat gctgggagaa gagttcacgt ttatcatgct gctttccggt
    24301 tcatggaatg ccatcactct gattgaatca acgttaccgt tgaaaggtgc cgaactggat
    24361 cttttaatcg tgatgaagcg cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc
    24421 tgggttcagg tcgatgttgc agactccccg catttaattg aacgcttcac agcacttttc
    24481 gacgcgcatc atatgaacat tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa
    24541 cgggctgcgc agttgcatat tcagataacc gcccacagcc ccgcatccgc ggacgcagca
    24601 aatattgagc aagcgttcaa agccctatgt acagaactca atgcacaagg cagtattaac
    24661 gtcgtcaatt attcccaaca tgatgaacag gatggagtta agtaatgaat ccactgaaag
    24721 ccggtgatat cgcaccgaaa tttagcttgc cggatcaaga cggagaacaa gttaatttga
    24781 ccgacttcca gggacagcgt gttctggttt atttctaccc gaaagccatg acccccggct
    24841 gtaccgtaca ggcctgcggc ttacgcgata acatggatga gttgaaaaaa gcgggcgttg
    24901 atgtgctggg tatcagcacc gataaacccg aaaaactctc ccgttttgcg gaaaaagagc
    24961 tgcttaactt tacgctcctg tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct
    25021 ggggtgaaaa gtccttcatg ggcaaaacct acgatggtat tcatcgcatc agcttcctga
    25081 ttgacgctga tggcaaaatc gaacatgtct ttgacgattt caaaaccagc aatcaccacg
    25141 acgttgtgct gaactggctg aaagaacacg cctgattact ttgctccatt ccgtgctggc
    25201 tgcgcttgcg gccagcatac ctcacttctc gtgatcaaga tcacattctc gctttcccct
    25261 gcgacacggg tgtcgaatcc attttttgct gaacgttaat gaccatcatt tttgtaccgt
    25321 tcagaatcca gttaatacat aacttattga atagattgag ttaatcagaa tggcatcctt
    25381 tatacaatat gaaatgcaat gtttcatatc attttcaagg agccgacatg aaccgctttg
    25441 tggtggccga accactgtgg tgtacaggat gtaatacctg tctcgctgcc tgttcggacg
    25501 tgcataaaac gcaaggttta cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa
    25561 tcactgcccc tgtcgtgtgt catcactgtg aggaagcccc ttgcctgcag gtctgcccgg
    25621 tcaatgccat ctctcagagg gatgatgcga tccaactcaa cgaaagcctc tgtattggct
    25681 gcaagctttg cgccgtggtc tgcccatttg gcgcaatcag cgcttcagga agccgtccgg
    25741 tgaatgccca tgcgcaatat gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa
    25801 acgtgccgcc ccaacatgcg ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga
    25861 aatgcgacct gtgtgatttc ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc
    25921 aggcgttacg gctgatcacc gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc
    25981 ttgccgcaag ctggtttgcc aatggcgggg agtctcccct ttccctcact caggagcaac
    26041 actaatggat gccctgcaat tattaacctg gtcgctgatc ctctatctgt ttgccagtct
    26101 ggcttcgctg tttttactcg gtctggacag actggctatt aagctttccg gcatcacatc
    26161 gctggtgggc ggcgtgattg gcatcatcag cggaattacg caattacatg ctggtgtaac
    26221 tttagtcgcc cattttgcca ccccttttga ctttgccgat ttaaccctgc gaatggatag
    26281 cctctcggca tttatggtgc tagttatctc cttgctggtg gtggtttgtt cgctctattc
    26341 attgacttat atgcgcgaat acgagggcaa aggcgcggcg gcgatgggct tctttatgaa
    26401 tcttttcatc gcatcgatgg ttgccctgct ggtgatggac aacgcttttt ggttcatcgt
    26461 gctgtttgaa atgatgtcgc tgtcttcctg gtttctggtc attgccaggc aggataaaac
    26521 gtcgatcaac gctggcatgc tctacttttt tatcgcccac gccggatcgg tgctgattat
    26581 gatcgccttc ttgctgatgg ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg
    26641 ctttcacttt ctccggggct ggcgtcggcg gtgtttctgc tggccttttt cggttttggc
    26701 gcgaaagccg ggatgatgcc gttgcacagc tggttgccgc gcgctcaccc tgccgcacca
    26761 tcgcacgctt cggcgttgat gtctggcgta atggtcaaaa tcggtatttt cggcatcctg
    26821 aaagtagcga tggatctgct ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg
    26881 atggcgatcg gcgcaatctc cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat
    26941 atcaaacggc tgctggcctg gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc
    27001 ggtgtggcga tggacggtct gtcactgcac gacccgctgc tcaccgtggt tggactgctc
    27061 ggcgcactgt ttcatctgct caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg
    27121 gacgcgatta tttcgcgttt gcatacccac gacatggaaa aaatgggggc actggcgaaa
    27181 cggatgccgt ggacagccgc agcatgcctg attggttgcc tcgcgatatc agccattcct
    27241 ccgctgaatg gttttatcag cgaatggtac acctggcagt cgctgttctc actaagtcgt
    27301 gtggaagccg tagcgctaca acttgcgggt cctattgcta tggtaatgct ggcagtcact
    27361 ggtgggctgg cagtaatgtg cttcgtcaaa atgtacggta ttactttctg tggtgcgccg
    27421 cgcagtacac acgctgaaga ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta
    27481 ctgctcgcgg cactctgcgt attaattgcg cttagtgcca gttggctggc accgaagata
    27541 atgcatattg cccatgcgtt taccaatacc cctcccgtca ctgtcgccag cggaatagca
    27601 cttgtacccg gcacgtttca tacacgggtc actccttcat tactgttgct gttactactg
    27661 gcgatgcctt tgctgccagg cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt
    27721 cgtcgcacag gagatgcctg ggcatgcggc tacggctggg aaaacgcgat ggctccgtca
    27781 ggcaatggcg tgatgcagcc gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa
    27841 cagctcgacc ctacgctgag gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag
    27901 agcacagaac ccttctggga tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg
    27961 ctggtcaaag aaatacagca tctgcaaagc ggcgactttc gtctctattg cctgtatgtg
    28021 gtcgccgcac tggttgtgct gctaatcgct attgccgtct aaggaaatca ccatgagaca
    28081 aactctttgc gacggatatc tggtcatttt tgcgttagca caggccgtga ttctgctgat
    28141 gctaacccca ctttttacgg gtatttcccg gcagatacgc gcgcgtatgc actcccgccg
    28201 cgggccgggg atctggcagg attatcgcga tatccacaaa ctgtttaaac gccaggaagt
    28261 tgcgccgaca tcttcaggtc tgatgttccg cctgatgccg tgggtattaa tcagcagcat
    28321 gctggtgctg gcgatggcct taccactatt tattaccgtt tccccttttg cgggcggcgg
    28381 cgatctgatc acccttatct atcttcttgc cctgtttcgt tttttctttg ctctttccgg
    28441 gctggatacc ggaagtccgt ttgcgggagt cggtgccagt cgcgagttga cgctcggcat
    28501 tctggtcgaa ccaatgctta ttctctcact gctggtattg gcgctgatag caggttccac
    28561 gcatatcgag atgatcagca atacgctggc tatgggctgg aactcgccgc taaccaccgt
    28621 actggcgtta ctggcctgtg gttttgcctg cttcattgag atgggaaaaa ttccctttga
    28681 tgttgctgaa gcagaacagg aattacagga aggcccgctg accgaatatt ccggtgccgg
    28741 gctggcgcta gcgaaatggg ggctggggct gaaacaggtc gtgatggcag cactgtttgt
    28801 ggccctgttt ctgccctttg ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc
    28861 acttgtcgtt acgctgctca aggttttgct gatttttgta ctggcctcaa tcgcagaaaa
    28921 cacgctggca cgcgggcgtt ttttactcat tcaccatgtg acctggcttg gcttcagcct
    28981 tgctgcgctt gcatgggtct tttggttaac cggtctgtaa ggagcactga cggaatatgg
    29041 aaaatcttgc tctgacgacg ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt
    29101 cgccacaacg tcgggccgcc gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca
    29161 tgttgtcgct gatctccgcg ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg
    29221 tcaacgtggg ggatgtggca ttgtttggcc aggtcattga tcgcgtgagt acgctgattc
    29281 tgtttgtggt ggtgtttctc ggtttgctgg tcacgatcta ctccacgggt tatctgacgg
    29341 ataaaaatcg cgaacacccg cataacggca cgaatcgtta ttacgcattt ttgctggtgt
    29401 ttatcggcgc gatggcggga ctggtagtct cctcgacgct gctcggtcag ttgttgtttt
    29461 ttgaaattac gggcggctgc tcctgggcgt tgatcagtta ttaccagagc gataaagcgc
    29521 agcgttcagc actaaaagcg ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg
    29581 ccgccgccac gctgtttttg cagaccggaa cgtttgtgct tagcgcgatg agcgagttac
    29641 atggcgacgc acgttatctg gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg
    29701 cccagctacc gatgcaagcg tggctaccgg acgcaatgga agcgccaaca ccgatcagcg
    29761 cctatctcca cgccgcatcg atggtgaaag tgggcgttta catttttgcc cgcgccatta
    29821 tcgacggcgg caatatcccg catgtgattg gcggcgttgg catggtcatg acactggtca
    29881 ccattcttta tggctttctg atgtatttgc cacagcagga tatgaagcgg ttgctggcct
    29941 ggtcgaccat cactcaactt ggctggatgt tcttcggctt gtcgctctcc atcttcggct
    30001 cgcggctggc gctggagggt agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc
    30061 tgtttttcct tgtagcaggt gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc
    30121 tgcgtggcgt attgcacacc ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg
    30181 caattaccgg tgtgccgccg ttcaacggct tcttcagtaa attcccgctg tttgccgccg
    30241 gttttgcgtt gtcagtggag tactggatcc tgctgcccgc catgattctg ctgatgattg
    30301 aatcggtcgc cagtttcgcc tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac
    30361 cgagcgaggc cgtcgccgat gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg
    30421 tactgattgt gatgtcgctg atttccagcg taatcgccgc gacctggttg cagtaaggag
    30481 atgatgaatg accggttcta tgatcgtaaa taatctggcg ggactgatga tgctgacatc
    30541 gctgtttgtg attagcgtca aaagctatcg cctgtcatgc ggattttacg cctgccagtc
    30601 gctggtgctg gtgtctattt tcgccactct ctcgtgcctg ttcgccgcag agcaactgct
    30661 gatctggtcc gccagcgcct ttatcaccaa agtgctgctg gtaccgttaa tcatgactta
    30721 cgctgcacga aatattcccc agaacatccc ggaaaaagcg ttattcggtc cggcaatgat
    30781 ggcactgctc gcggcgttaa ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct
    30841 accgatggct accgggctga aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg
    30901 cctgctgtgc attgtcagcc aacgcaatat cctgcggcaa atttttggtt actgcctgat
    30961 ggaaaacggc tcccatctgg tgctggcgct tcttgcctgg cgagcaccgg aactggtgga
    31021 aataggtatc gctaccgacg ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa
    31081 aatatggcgt acccacggca cgctggacgt gaacaacttg accgcgctga agggataatg
    31141 agatgagtta ttctgtgatg ttcgctttac tcctgctcac gccgctgctt ttttcgctgc
    31201 tctgttttgc ctgccggaaa cgggggctct ctgcgacttg tacggtgact gtattacata
    31261 gcttagggat cacactgctg ctgattctgg cactctgggt ggtccaaact gccgctgatg
    31321 caggagaaat attcgctgcg ggactgtggc ttcatattga tggtctgggc ggtttgttcc
    31381 tcgccattct tggtgtgatt ggctttctca ccggtgttta ctcgattggc tacatgcgtc
    31441 atgaagtggc acacggcgag ctttcacccg ttacactgtg cgattactac ggtttcttcc
    31501 atctgttttt gttcaccatg ctgctggttg ttaccagcaa taacctgatt gtgatgtggg
    31561 cggcgatcga agccaccacc ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt
    31621 catcgctgga atcagcatgg aagtacatca ttatttgtac tgttggtgtc gcttttggtc
    31681 tgttcggtac cgtgctggta tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg
    31741 cgatattctg gagcgaggtt cttaagcaat cgtccttgct tgacccaaca ttaatgctgt
    31801 tggcctttgt gtttgtgcta attggctttg gtaccaaaac cgggctattt cccatgcacg
    31861 cctggctgcc ggatgctcac agtgaagcgc cgagtccggt cagcgccctg ctctccgccg
    31921 tattgctgaa ctgcgcgctg ttggtgctga ttcgctatta catcattatt tgccaggcca
    31981 tcggcagcga tttccccaac cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg
    32041 cggcattttt cattctggta cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg
    32101 agaacatggg gctggtcgcg gtggcgctag gcattggcgg gccgctggga atttttgccg
    32161 cgctgctgca caccttaaac cacagtctgg caaaaacgct gctgttctgc ggttccggca
    32221 atgtactgct caagtacggc acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca
    32281 tgccatttac cgccgtgctg tttggcggcg gtgcgctggc gctggcgctg gcagggatgc
    32341 cgcccttcaa catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc
    32401 acctgctgat tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac
    32461 ggatggctgc gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg
    32521 gctggttgac cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc
    32581 atattccaca acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga
    32641 cgcacgacct gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat
    32701 ctgtttcgga gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc
    32761 gccgccctga aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa
    32821 caggtcacca ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat
    32881 caacatgatg gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat
    32941 tacgcggttt attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag
    33001 gcactggtcg atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg
    33061 gtctggggcg agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat
    33121 cagcgtcgcc tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat
    33181 gcgatggatt atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc
    33241 aatgagggca acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat
    33301 gaaccgggtc acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc
    33361 ctgttttatg tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa
    33421 gtgaccttct tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat
    33481 accaactcgg ttaaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc
    33541 tcgattctgc tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc
    33601 cattttgttg gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg
    33661 acgatggcgg aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt
    33721 gttcgccgcg atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg
    33781 cgcgccgacg tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc
    33841 actcagggca ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg
    33901 ctgatccgcg cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg
    33961 gcaatattcc aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg
    34021 tccgtgtcaa agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc
    34081 cggatacccc actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct
    34141 ttgttgaagc gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat
    34201 tgttccgctg gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc
    34261 tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact
    34321 cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt
    34381 ataaagagat cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga
    34441 agatgctgaa gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct
    34501 tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat
    34561 gtattgcctg cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg
    34621 acgaccagca aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac
    34681 gttgtgaaga agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg
    34741 tcacccataa agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt
    34801 gcgaacgccc gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc
    34861 aacaaaatgc tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt
    34921 gcaaacaacg cgcgacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc
    34981 agctatgagt ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac
    35041 gcaaaagatg aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat
    35101 cgattgcggc ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt
    35161 cgacgcagaa cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt
    35221 atttactggc acagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc
    35281 ccccgatcat aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca
    35341 cgatctctac agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc
    35401 cggctgcccg ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct
    35461 gcaacagaag attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt
    35521 gtggccgcag atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc
    35581 gggctatcgt caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc
    35641 tacaggaaat cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat
    35701 cgttcagcaa ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg
    35761 ttttctggac gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct
    35821 ctcaggtaat gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga
    35881 atgtcgcctt ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg
    35941 agcaagcccg acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca
    36001 gccacaccgc cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt
    36061 ggcaagcgcg tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta
    36121 tctatctgat ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat
    36181 ggctatgtca gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa
    36241 cggaatgctc gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt
    36301 catcccgctg ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag
    36361 caacttctat taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca
    36421 gggcgagatg gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga
    36481 gctacgcttt attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa
    36541 ctggcaggca cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca
    36601 ggtggcgctg cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac
    36661 taatgcagtg ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat
    36721 ccatcatttt ttcggtctgt cttcagtcag tatggtactg ggcgatcatc gaaagaacga
    36781 gaagttcagc ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa
    36841 tatgcctggc gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac
    36901 gcaccgtgca gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc
    36961 taacggctgc gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc
    37021 attgttgctg gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca
    37081 acacatagcc gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac
    37141 cgatttgcag gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa
    37201 tctgggcgtc ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt
    37261 agatattgtg gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa
    37321 agaggtgatc gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa
    37381 aatcaactgc gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa
    37441 aggggcgttt actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg
    37501 cacgttgttt ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg
    37561 cgtattgcag gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt
    37621 cagagtcatt gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg
    37681 cagcgatctc ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg
    37741 tccggaagat atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa
    37801 tcgttcaatt gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc
    37861 gggcaacgtg cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa
    37921 cagtctgaat ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga
    37981 ttcagcgctt cgcagttcaa tggcgcagtt gctgcacccg acgacgccag agaatgacga
    38041 agaagaacgt cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc
    38101 ccgtggcgcg gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg
    38161 tctgggaatc tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa
    38221 caaactctct ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc
    38281 cgcccataaa attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt
    38341 gtttatggcg atcggattta ctttttacct ttccgttatc gccgatgccc cgtcttcata
    38401 ggcattaacc catctggtgg gcggcctttg ctttacactt ggctttattt tgctggcggt
    38461 ttgcggcacc agcctgttca cctcgtcggt aatgacggta atggcaaaaa gtcggggcgt
    38521 tattagttgg cgaacttggc tgattaacgc acttctggtg gcctgcggta atctggcagg
    38581 tattgcctgt ttcagtttgt taatctggtt ttccgggctg gtgatgagtg aaaacgcgat
    38641 gtggggagtc gcgattttac actgcgccga gggcaaaatg catcatacat ttactgaatc
    38701 tgtcagcctc ggcattatgt gcaatctgat ggtttgcctg gcgctgtgga tgagttattg
    38761 cggacgttcg ttatgcgaca aaatcgtcgc catgattttg tccatcaccc tgtttgtcgc
    38821 cagtggcttt gagcactgta tcgccaattt gtttgtgatt ccgttcgcca ttgccattcg
    38881 ccatttcgcc cctacctcct tctggcaact ggcgcacagt agcgcagaca attttccggc
    38941 actgacggtc agccatttta ttaccgccaa tctgctcccg gtgatgctgg gtaatattat
    39001 cggcggtgca gtgctggtga gtatttgtta tcgggctatt tatttacgtc aggaatcctg
    39061 aaaaatcagc ccggcgaaac agttcgtcgg gctgaagatt attcttgcgc gatttgccca
    39121 tcgggccagg cgtggatcac ggctttgatc agcgtcgcca atggaatggc gaagaatacg
    39181 ccccagaatc cccacaaacc accgaagatc accaccgata aaataatcac cagcggatgc
    39241 aggttaaccg cttcggagaa caacaccggt actagcaggt tgccgtccag cgcctgaata
    39301 atcagataca ccgcgaagca gctccagaat tcagtacctg cgccaaactg gaatagcgcc
    39361 acgccaacca ccggaatggt caccacaaat gcgccaatgt acggaataag aaccgagaag
    39421 ccgaccagca ctgccagcag cagcgaatag ttcagcccga agagcaagaa ccccagccag
    39481 gtggcgatcc ccaccacgat catctccagc actttgccgc ggatatagtt ggtgatttgt
    39541 tgattcatct ccttccacac ctgtcctgcc agcccacggt tacgcggcag cacccggcga
    39601 acggcgttca gcatctgctc tttgtctttc agcaggaaga agaccatcaa tggcaccagc
    39661 accagataga cggctatggt cagcaaaccg accagcgagg cgagggaaat tttcaccacc
    39721 gaatcgccca tggtcagcat ccgactgcgc atattttcgg ccattgcatc aataatgccc
    39781 gcgtccatta acaccggata gcggcgcggc aacgtggcgg caaagtcaga aagcttattg
    39841 agcatccccg gcatatcgcg gattaagtag atgccctgtt gccaggcgat aggcagtacc
    39901 acgaacgcca tcagtagcaa tataccgaca aaaaccacca atacaatcga cgtcgcccag
    39961 cggcgggagc agccaataga ttgcaggcgc acggttggcc attccagcaa ataagccagc
    40021 acaatagcca ccagcaacgg agcaagcagg ccactaaaga aaaagataat gccaaatccg
    40081 gcaactaaaa taaccagcaa ggcaatcgct tccgggtcgc taaaacggcg gcgataccat
    40141 tgcatcaaca tttcgagcat aaagaccttc cctgaacctc aagagcggga ttgcgatccg
    40201 caattgtatc gaaatgtcac aaaaaagact tcgcttttta tgacggattc aggaaactaa
    40261 aaagtcattt gagtgggcta atcttcgccg ttacactcaa aggcggcgag gtgggaacga
    40321 tatttcacag tatcggtcaa atgactatct tcagaaatac aggatagagg ttatgttcag
    40381 gcagttgaaa aaaaacctgg ttgcaaccct cattgctgct atgaccattg gtcaggtagc
    40441 cccggcgttt gccgacagcg cagacacctt gccggatatg ggaacctccg caggaagcac
    40501 gctttccatt ggtcaggaaa tgcagatggg cgactattat gtccgccagc tacgcggcag
    40561 cgcgccgtta attaatgacc cgctgttaac gcaatatatt aattcgctgg ggatgcgtct
    40621 ggtttcgcat gccaattcgg ttaagacacc gtttcatttt tttctgatca acaacgacga
    40681 aattaacgcc tttgctttct ttggcggcaa cgtggtgctg cactctgccc tgttccgtta
    40741 ttccgataac gaaagtcaac tggcttcagt tatggcgcac gaaatctccc acgtcaccca
    40801 acgtcacctg gcgcgagcga tggaagatca acaacgcagc gcgccgctga cctgggtcgg
    40861 cgcgttaggt tctattttac tggcgatggc cagtccgcag gcggggatgg cggcgctgac
    40921 cggtacactg gcgggaacgc gccaggggat gatcagtttc acccagcaaa atgaacagga
    40981 agcggaccgc attggtattc aggtgctgca acgctcggga ttcgatccac aggcgatgcc
    41041 gaccttcctg gaaaaattgc tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt
    41101 attgactcac ccgttgccgg aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat
    41161 gcgcccgatg gtggtgcagt cgtcggaaga tttctatctg gcgaaagcgc gcacactggg
    41221 gatgtataat tccggacgta accagctcac cagtgatttg ctggatgaat gggcgaaagg
    41281 aaacgttcgt cagcaacgag cggcgcaata tggtcgtgct ttacaggcga tggaagccaa
    41341 taaatacgac gaggcgcgta aaacgctgca accgttactg gcggcagaac ctggcaacgc
    41401 atggtatctc gatctggcta ccgatatcga tcttgggcaa aacaaagcca atgaggcaat
    41461 caatcgcctg aaaaatgccc gcgatttacg caccaatccg gtgttgcagc tcaacctggc
    41521 gaacgcttat ctgcaaggcg gtcaaccaca agaagcggcc aatattctga atcgctacac
    41581 ctttaataat aaagatgaca gcaacggctg ggatttgctg gcacaggcgg aagccgcgct
    41641 aaataaccgc gatcaggagc tggctgcgcg agcagaaggt tatgcgctcg ccggacgact
    41701 cgatcaggcc atttcgctgt tgagtagcgc cagttcgcag gtaaaattag gcagcctgca
    41761 acaagcgcgt tacgatgcgc gcatcgacca gttgcgccag ctgcaggaac gctttaagcc
    41821 ttataccaag atgtaattcg cattaaagga agaacaatga ccaaacaggt aaaaatctac
    41881 cataacccac gctgttcaaa gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg
    41941 gagccagaag tggtgctcta ccttgagaca cccgccgatg cggcaacgct gcgcgatttg
    42001 ctgaaaatgc tggggatgaa tagcgcccgt gaattgatgc gccagaaaga ggatctttat
    42061 aaagaactaa acctggctga cagttcgctt agcgaagagg cgctgattca ggcaatggtg
    42121 gataatccaa agctgatgga gcgcccgatt atggtcgcga atggcaaagc gcggattggt
    42181 cgtccaccgg agcaggtact ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag
    42241 cacattgccg gatgcgacgc ttgccacgtc ttatccggcc tacgaatact atggattaac
    42301 ttcgcgtagg tcaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag
    42361 aatttctttc acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa
    42421 ctgatccaac gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg
    42481 ccccacatct tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa
    42541 cttatcttca tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gacgcgacgc
    42601 gagatccggt aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaaccg
    42661 tgttttgccc gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc
    42721 atcgcctgca atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc
    42781 gagcacttcc ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc
    42841 acgctgcgac aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg
    42901 tgcccagaga tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcggccag
    42961 taaagaggag ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag
    43021 tggcaaagag agctgtgcct gtgtgttcag agatacctca accaggattt cacaaaatcg
    43081 cgagaagttt accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg
    43141 tacattattt gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca
    43201 gcacactgat cagtttgaaa atcaaactta agccgatacc gacgatggtc gccagcgcca
    43261 taccttttaa ctccgccgcg ccgatgttta ccttcgcgcc actgacgccg atgatcaaaa
    43321 tcacggaagt caggatcagg ttctgtgctt tgttgtaatc cactttcgat tcgatcaaaa
    43381 cacgaatacc ggaagcacca atgacaccat aaagcagcag cgaaacgccg cccatcaccg
    43441 gcaatgggat catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga
    43501 aaatcgccgc cccgccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa
    43561 tgttttctcc gtaagtggta tttggcgtag agccaaagaa gccggagatc acggtcgaca
    43621 ggccgttagc aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga
    43681 tattagccgt tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca
    43741 gaatagtcag aatggcaaac cactcgaagc gcggcgtata gagggttggc agtgcaaacc
    43801 agtgagcatt aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc
    43861 ccaccagcac gccaattaaa atcgggataa ttgccaggaa accacgaaac agcacggaac
    43921 ctaaaaccgt gaccgccagg gtggtaatag agatgatgat ggttttggag tttggcgttt
    43981 gcccttcagc cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga
    44041 tgacggcaac gattgcgccc attgccgcag gtggaaacag cacgtccaac cagccggtcc
    44101 ccgctttctt cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc
    44161 ccagcgcgac ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc
    44221 tggaaccaag ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa
    44281 taccgttaaa taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg
    44341 ttgcaccaaa catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg
    44401 gtctttcact caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta
    44461 taggcgcttt actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt
    44521 tatttcgtac caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc
    44581 tcgttcagtc cctggtcaat cgatgcagta tacagttcga cgtccgggtg cgctttttcc
    44641 agcgcagcga taccttctgg cgcagcgacc agcaccagaa ctttgatgct gctgcagccc
    44701 gcttttttca gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca
    44761 acgatcagcg ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc
    44821 tccagcgttt cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacgttt
    44881 tccagcacac cgtccatcat accaagaccc gcacgcagaa ttggcacaac ggtaattttc
    44941 ttacctttga tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc
    45001 gtttcgaggt cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg
    45061 cgaaagcgct tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt
    45121 gggtgtttga cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa
    45181 atcgacggat tatacctcct ttcttcaagg cggcaatatt attttcgttg actttagtca
    45241 aaatgataac agtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat
    45301 tccatccgaa aactaacctt tgccctggca caagtcttct ttcgccgcgc gcccggggaa
    45361 aagacgtgca aaaaggttgt gtaaaacagt ctcgcaaacg tttgctttcc ctgttagaat
    45421 tgcgccgaat tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata
    45481 aaacctctct tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg
    45541 gaagaatcaa aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg
    45601 gcttcggtgc gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca
    45661 ctgacggcgt aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg
    45721 gtattgatct ggtcgccatg tgcgttaatg acctggtggt gcaaggtgcg gaaccgctgt
    45781 ttttcctcga ctattacgca accggaaaac tggatgttga taccgcttca gcggtgatca
    45841 gcggcatcgc ggaaggttgt ctgcaatcgg gctgttctct ggtgggtggc gaaacggcag
    45901 aaatgccggg gatgtatcac ggtgaagatt acgatgtcgc gggtttctgc gtgggcgtgg
    45961 tagaaaaatc agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac
    46021 tcggttccag cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca
    46081 gcggttgtga tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg
    46141 caccgacccg catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg
    46201 ccattgcgca cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata
    46261 atacccaggc agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc
    46321 aaacggcagg taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga
    46381 tgattatcgc cctgcctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg
    46441 gtgaaaacgc gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta
    46501 tcgaataatg aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat
    46561 tgacgcctgt aaaaccaaca aaattaaagg caccgtacgg gcagttttca gcaataaggc
    46621 cgacgcgttc ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc
    46681 cagcgcgttt gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta
    46741 cgcacccgat gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc
    46801 ccactatgcc gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt
    46861 acacacccat cgtcaggcgc tggaaaatgg cgatgaagag cacggtacat cggtgcattt
    46921 cgtcaccgat gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc
    46981 tggtgatacg gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc
    47041 actggtgatt agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct
    47101 ggatggtcaa cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc cccgtagtta
    47161 aagcgccagc tctgccgctg gcgtttttca attcacctgt aaatcgcaag ctccagcagt
    47221 ttttttcccc cttttctggc atagttggac atctgccaat attgctcgcc ataatatcca
    47281 ggcagtgtcc cgtgaataaa acggagtaaa agtggtaatg ggtcaggaaa agctatacat
    47341 cgaaaaagag ctcagttggt tatcgttcaa tgaacgcgtg cttcaggaag cggcggacaa
    47401 atctaacccg ctgattgaaa ggatgcgttt cctggggatc tattccaata accttgatga
    47461 gttctataaa gtccgcttcg ctgaactgaa gcgacgcatc attattagcg aagaacaagg
    47521 ctccaacgct cattcccgcc atttactggg caaaattcag tcccgggtgc tgaaagccga
    47581 tcaggaattc gacggcctct acaacgagct attgctggag atggcgcgca accagatctt
    47641 cctgattaat gaacgccagc tctccgtcaa tcaacaaaac tggctgcgtc attattttaa
    47701 gcagtatctg cgtcagcaca ttacgccgat tttaatcaat cctgacactg acttagtgca
    47761 gttcctgaaa gatgattaca cctatctggc tgtggaaatt atccgtagcg atagcatccg
    47821 ttacgcgctg ctggagatcc catcagataa agtgccgcgc tttgtgaatt taccgccaga
    47881 agcgccgcgt cgacgcaagc cgatgattct tctggataac attctgcgtt actgccttga
    47941 tgatattttc aaaggcttct ttgattatga cgcgctgaat gcctattcaa tgaagatgac
    48001 ccgcgatgcc gaatacgatt tagtgcatga gatggaagcc agcctgatgg agttgatgtc
    48061 ttccagtctc aagcagcgtt taactgctga gccggtgcgt tttgtttatc agcgcgatat
    48121 gcccaatgcg ctggttgaag tgttacgcga aaaactgact atttcccgct acgactccat
    48181 cgtccccggc ggtcgttatc ataattttaa agactttatt aatttcccca atgtcggcaa
    48241 agccaatctg gtgaacaaac cactgccgcg tttacgccat atttggtttg ataaagccca
    48301 gttccgcaat ggttttgatg ccattcgcga acgcgatgtg ttgctctatt atccttatca
    48361 cacctttgag catgtgctgg aactgctgcg tcaggcttcg ttcgacccga gcgtactggc
    48421 gattaaaatt aacatttacc gcgtggcgaa agattcacgc atcatcgact cgatgatcca
    48481 cgccgcacat aacggtaaga aagtcactgt ggtggttgag ttacaggcgc gtttcgacga
    48541 agaagccaac attcactggg cgaagcgcct gaccgaagca ggcgtgcacg ttatcttctc
    48601 tgcgccgggg ctgaaaattc acgccaaact gttcctgatt tcacgtaaag aaaacggtga
    48661 agtggtccgt tacgcacaca tcgggaccgg gaactttaac gaaaaaaccg cgcgtcttta
    48721 tactgactat tcgttgctga ccgcagatgc gcgtatcacc aacgaagtac ggcgggtatt
    48781 taactttatt gaaaacccat accgcccggt gacatttgat tatttaatgg tgtcaccgca
    48841 aaactctcgc cgtctgttat atgaaatggt agaccgcgaa atcgccaacg cgcagcaagg
    48901 gctgcccagt ggtatcaccc tgaagctaaa taaccttgtc gataaaggcc tggttgatcg
    48961 tctgtatgcg gcctccagct ccggcgtacc ggttaatctg ctggttcgcg gaatgtgttc
    49021 gctgatcccc aatctggaag gcattagcga caacattcgt gccatcagta ttgttgaccg
    49081 ttaccttgaa catgaccggg tttatatttt tgaaaatggc ggcgataaaa aggtctacct
    49141 ttcttccgcc gactggatga cgcgcaatat tgattatcgt attgaagtgg cgacaccgct
    49201 gctcgatccg cgcctgaagc agcgggtgct ggacatcatc gacatattgt tcagcgatac
    49261 ggtcaaagca cgttatatcg ataaagaact cagtaatcgc tacgttcccc gcggcaatcg
    49321 ccgcaaagta cgggcgcagt tggcgattta cgactacatc aaatcactcg aacaacctga
    49381 ataaccctat gccaatacac gataaatccc ctcgtccgca ggagtttgct gcggtcgatc
    49441 ttggttcaaa cagttttcac atggtcatag cccgtgtggt agatggtgcc atgcagatta
    49501 ttggccgcct gaaacagcgg gtgcatctgg cggacggcct ggggccagat aatatgttga
    49561 gtgaagaggc aatgacgcgc ggtttgaact gtctgtcgct gtttgccgaa cggctacaag
    49621 ggttttctcc tgccagcgtc tgtatagtcg gcacccatac gctgcgtcag gcgctgaacg
    49681 ccactgactt tctgaaacgc gcggaaaagg tcattcccta cccgattgaa attatttccg
    49741 gtaatgaaga agcccgtctg atttttatgg gcgtggaaca tacccaaccg gaaaaaggtc
    49801 gcaaactggt tattgatatt ggcggcggat ctacggaact ggtgattggt gaaaatttcg
    49861 aacctattct cgttgaaagc cgccggatgg gttgtgtcag ctttgcccag ctttattttc
    49921 ctggcggggt catcaataaa gagaattttc agcgcgctcg catggcggca gcacaaaaac
    49981 tggaaacttt aacctggcaa ttccgtattc agggctggaa cgttgcaatg ggcgcttccg
    50041 gtaccataaa agccgcccat gaagtgttaa tggaaatggg cgagaaagac gggataatta
    50101 ccccggaacg tctggaaaaa ctggtaaaag aaattttacg tcaccgtaat ttcgcatcgc
    50161 tgagtttacc gggtctttcc gaagagcgga aaacagtctt cgttcctgga ctggcgattt
    50221 tatgtggtgt gtttgatgct ttagccatcc gtgaactgcg cctttctgac ggggcgttac
    50281 gcgaaggcgt actgtatgaa atggaaggac gtttccgtca tcaggatgtg cgtagtcgca
    50341 ccgccagcag cctcgccaac cagtatcaca tagacagcga acaggcccga cgagtgctgg
    50401 ataccactat gcaaatgtac gaacagtggc gggaacagca accgaagctg gcgcatccgc
    50461 aactggaggc gctactgcga tgggccgcca tgctgcatga ggtcgggttg aatatcaacc
    50521 acagcggttt gcatcgccac tccgcttata ttctgcaaaa cagtgacttg ccgggtttta
    50581 atcaggaaca gcagctgatg atggcgacac tggtgcgcta tcaccgtaaa gcgattaagc
    50641 tcgacgatct gccgcgcttt accttgttta agaagaaaca gttcctgcca ctgatacagc
    50701 tattgcgcct tggcgtatta ctaaacaatc aacgtcaggc aaccaccaca ccgccaacat
    50761 tgacactgat tactgatgac agtcactgga cactgcgttt cccgcatgac tggtttagtc
    50821 agaatgcgct ggtactgctt gatctggaaa aggagcaaga atactgggaa ggcgtggctg
    50881 gctggcggtt gaaaattgaa gaagaaagta ccccagaaat cgccgcttaa tgctttgcgg
    50941 gccgacgaga aatgtcggcc cgcattattc aggcactttc gcgaatgggt tcgatttcat
    51001 tcagcgtatc aattaacggt tgcggcttac caataagata accctgcata taatcgatcc
    51061 ccaaagagag caccgcctcg cggatctctt cgttttcaac gtactctgcc actacccgca
    51121 ttttcttcat tcgcgccaga tggcaaatcg acgccacaat ctgataatcc agactatttg
    51181 acacaatatt gcggataaag ctgccgtcaa ttttaagcag atcggcattc acatttttaa
    51241 gccgtgcata gctggcgtag ccggtgccga aatcatcaat cgcaatctgg cagcctaatt
    51301 cctgaagatg ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca ttactttcgg
    51361 tgacttcaaa aataagttgc cacgcttcaa tctgatattt agccagcaac tgactgactt
    51421 caacaggaaa acgagcctga cataccgagg ttggagacag attaatagca aaacggtgag
    51481 cgggcatctt cgctctgttt tcagccataa attgcagcgt atgctcaatg acccacatgt
    51541 cgatactcga cgataaacca aattcgtgcg cgaccggcaa gaagctaccg gggccgatca
    51601 gttcatcatt ctcacctttc atgcgcagaa gaatttcatg gtaaacatca ccacgcatac
    51661 cggtaatcgg ctgggccatc aggaaaaaat ggttgtgttc cagcgcctgc tgtagtcgat
    51721 tcatcatcgc gactttatct ttcaattcgc gttgcaaata cattgccccg caacgctgca
    51781 tattttccgg ggcgttggtc acgatggaaa gttcggcgac cgtatttagc tctcccagca
    51841 gcaggtagat atgattcact ggcgagcgca catagcagta actgacgcca atctgcggtt
    51901 gcatcggcat cccatcccaa aagaaacgaa attgcttgag atgactatcc agtgcggtaa
    51961 tgcgctcctg gtgcgattct gtattcagtc gcagcgcgag atcgttaccc gaaagctgat
    52021 aaacatcttc acccggttcc agcaagggtg acagccagtg agaaagtttt tgctt
//
LOCUS       DAEEGA010000021        50084 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEEGA010000021 DAEEGA010000000
VERSION     DAEEGA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50084
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.021"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..579
                     /locus_tag="KLH46_001274"
     CDS             <1..579
                     /locus_tag="KLH46_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HBE0378025.1"
                     /translation="IKVKRYRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVN
                     GRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQY
                     RMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVT
                     EYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            681..833
                     /gene="hokA"
                     /locus_tag="KLH46_001275"
     CDS             681..833
                     /gene="hokA"
                     /locus_tag="KLH46_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBE0378026.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(1020..1232)
                     /gene="cspA"
                     /locus_tag="KLH46_001276"
     CDS             complement(1020..1232)
                     /gene="cspA"
                     /locus_tag="KLH46_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBE0378027.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1513..1803)
                     /locus_tag="KLH46_001277"
                     /pseudo
     CDS             complement(1513..1803)
                     /locus_tag="KLH46_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2237..2947
                     /locus_tag="KLH46_001278"
     CDS             2237..2947
                     /locus_tag="KLH46_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBE0378028.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2997..3971)
                     /gene="ghrB"
                     /locus_tag="KLH46_001279"
     CDS             complement(2997..3971)
                     /gene="ghrB"
                     /locus_tag="KLH46_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBE0378029.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4075..4725)
                     /locus_tag="KLH46_001280"
     CDS             complement(4075..4725)
                     /locus_tag="KLH46_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBE0378030.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4888..7221
                     /gene="bisC"
                     /locus_tag="KLH46_001281"
     CDS             4888..7221
                     /gene="bisC"
                     /locus_tag="KLH46_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBE0378031.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(7190..7630)
                     /locus_tag="KLH46_001282"
     CDS             complement(7190..7630)
                     /locus_tag="KLH46_001282"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE0378032.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7627..8190)
                     /gene="tag"
                     /locus_tag="KLH46_001283"
     CDS             complement(7627..8190)
                     /gene="tag"
                     /locus_tag="KLH46_001283"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBE0378033.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8348..9046
                     /locus_tag="KLH46_001284"
     CDS             8348..9046
                     /locus_tag="KLH46_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE0378034.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9275..10483
                     /locus_tag="KLH46_001285"
     CDS             9275..10483
                     /locus_tag="KLH46_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0378035.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10807..12498
                     /gene="eptB"
                     /locus_tag="KLH46_001286"
     CDS             10807..12498
                     /gene="eptB"
                     /locus_tag="KLH46_001286"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBE0378036.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12590..12666
                     /locus_tag="KLH46_001287"
     tRNA            12590..12666
                     /locus_tag="KLH46_001287"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12624..12626,aa:Pro,seq:cgg)
     gene            13410..15017
                     /gene="dppA"
                     /locus_tag="KLH46_001288"
     CDS             13410..15017
                     /gene="dppA"
                     /locus_tag="KLH46_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBE0378037.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15325..16344
                     /gene="dppB"
                     /locus_tag="KLH46_001289"
     CDS             15325..16344
                     /gene="dppB"
                     /locus_tag="KLH46_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBE0378038.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16354..17256
                     /gene="dppC"
                     /locus_tag="KLH46_001290"
     CDS             16354..17256
                     /gene="dppC"
                     /locus_tag="KLH46_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBE0378039.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17267..18250
                     /gene="dppD"
                     /locus_tag="KLH46_001291"
     CDS             17267..18250
                     /gene="dppD"
                     /locus_tag="KLH46_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBE0378040.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18247..19251
                     /gene="dppF"
                     /locus_tag="KLH46_001292"
     CDS             18247..19251
                     /gene="dppF"
                     /locus_tag="KLH46_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBE0378041.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19281..20552)
                     /locus_tag="KLH46_001293"
     CDS             complement(19281..20552)
                     /locus_tag="KLH46_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBE0378042.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            21027..21134
                     /locus_tag="KLH46_001294"
     CDS             21027..21134
                     /locus_tag="KLH46_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE0378043.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21510..21617
                     /gene="ldrD"
                     /locus_tag="KLH46_001295"
     CDS             21510..21617
                     /gene="ldrD"
                     /locus_tag="KLH46_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HBE0378044.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21993..22100
                     /locus_tag="KLH46_001296"
     CDS             21993..22100
                     /locus_tag="KLH46_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE0378045.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            22476..22583
                     /locus_tag="KLH46_001297"
     CDS             22476..22583
                     /locus_tag="KLH46_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE0378046.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(22670..24349)
                     /gene="bcsG"
                     /locus_tag="KLH46_001298"
     CDS             complement(22670..24349)
                     /gene="bcsG"
                     /locus_tag="KLH46_001298"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBE0378047.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(24346..24537)
                     /gene="bcsF"
                     /locus_tag="KLH46_001299"
     CDS             complement(24346..24537)
                     /gene="bcsF"
                     /locus_tag="KLH46_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBE0378048.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(24534..26105)
                     /gene="bcsE"
                     /locus_tag="KLH46_001300"
     CDS             complement(24534..26105)
                     /gene="bcsE"
                     /locus_tag="KLH46_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBE0378049.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            26378..26566
                     /locus_tag="KLH46_001301"
     CDS             26378..26566
                     /locus_tag="KLH46_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBE0378050.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            26578..27330
                     /gene="bcsQ"
                     /locus_tag="KLH46_001302"
     CDS             26578..27330
                     /gene="bcsQ"
                     /locus_tag="KLH46_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBE0378051.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLCYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            27327..27686
                     /locus_tag="KLH46_001303"
     CDS             27327..27686
                     /locus_tag="KLH46_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HBE0378052.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            27683..29944
                     /gene="bcsA"
                     /locus_tag="KLH46_001304"
                     /pseudo
     CDS             27683..29944
                     /gene="bcsA"
                     /locus_tag="KLH46_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            29955..32294
                     /gene="bcsB"
                     /locus_tag="KLH46_001305"
     CDS             29955..32294
                     /gene="bcsB"
                     /locus_tag="KLH46_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBE0378053.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISSRRLNPDNE"
     gene            32301..33407
                     /gene="bcsZ"
                     /locus_tag="KLH46_001306"
     CDS             32301..33407
                     /gene="bcsZ"
                     /locus_tag="KLH46_001306"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBE0378054.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            33466..33642
                     /locus_tag="KLH46_001307"
                     /pseudo
     CDS             33466..33642
                     /locus_tag="KLH46_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            34132..34293
                     /locus_tag="KLH46_001308"
     CDS             34132..34293
                     /locus_tag="KLH46_001308"
                     /inference="COORDINATES: protein motif:HMM:NF025910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378055.1"
                     /translation="MTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNTSLNT
                     QRRSRRLIS"
     gene            34290..35675
                     /gene="bcsC"
                     /locus_tag="KLH46_001309"
     CDS             34290..35675
                     /gene="bcsC"
                     /locus_tag="KLH46_001309"
                     /inference="COORDINATES: protein motif:HMM:NF008520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HBE0378056.1"
                     /translation="MIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEVNGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            35757..37745
                     /gene="hmsP"
                     /locus_tag="KLH46_001310"
     CDS             35757..37745
                     /gene="hmsP"
                     /locus_tag="KLH46_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBE0378057.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNFSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            37930..39216
                     /gene="dctA"
                     /locus_tag="KLH46_001311"
     CDS             37930..39216
                     /gene="dctA"
                     /locus_tag="KLH46_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBE0378058.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            39437..40933
                     /locus_tag="KLH46_001312"
     CDS             39437..40933
                     /locus_tag="KLH46_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBE0378059.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(41029..41958)
                     /gene="kdgK"
                     /locus_tag="KLH46_001313"
     CDS             complement(41029..41958)
                     /gene="kdgK"
                     /locus_tag="KLH46_001313"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBE0378060.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            42190..42957
                     /gene="pdeH"
                     /locus_tag="KLH46_001314"
     CDS             42190..42957
                     /gene="pdeH"
                     /locus_tag="KLH46_001314"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBE0378061.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            43027..45087
                     /locus_tag="KLH46_001315"
     CDS             43027..45087
                     /locus_tag="KLH46_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBE0378062.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(45269..46591)
                     /locus_tag="KLH46_001316"
     CDS             complement(45269..46591)
                     /locus_tag="KLH46_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBE0378063.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(47002..48015)
                     /gene="yhjD"
                     /locus_tag="KLH46_001317"
     CDS             complement(47002..48015)
                     /gene="yhjD"
                     /locus_tag="KLH46_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBE0378064.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(48064..48963)
                     /locus_tag="KLH46_001318"
     CDS             complement(48064..48963)
                     /locus_tag="KLH46_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0378065.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            49483..>50084
                     /locus_tag="KLH46_001319"
     CDS             49483..>50084
                     /locus_tag="KLH46_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE0378066.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT        12035 a        12471 c        13501 g        12077 t
ORIGIN      
        1 attaaggtca agcgataccg ctcttacaga ggagaggtag ggcaaaccgc ccctaatgtt
       61 ctccaaagag atttcaaggc tacgcggcca aacgagaagt gggttaccga tgttactgaa
      121 tttgcagtca atgggcgcaa gctgtatttg tctccagtaa tagatctctt caacaacgaa
      181 gttatttctt acagcctttc ggaaagacca gtgatgaaca tggttgagaa tatgctcgat
      241 caggcattca aaaagcttaa tcctcacgag catcctgttc tgcactctga ccagggatgg
      301 cagtatcgta tgagaagata tcaaaatatc cttaaagaac atggtattaa acaaagcatg
      361 tccagaaaag gcaattgtct ggataatgct gtggtggagt gtttctttgg aaccttaaag
      421 tcggagtgtt tttatcttga tgagttcagt aatataagcg aactgaagga tgctgttacg
      481 gaatatattg aatactataa cagcagaaga attagcctga aattaaaagg tctgactcca
      541 attgaatatc ggaatcagac ctatatgcct cgtgtttaac tgtccaactt tttggggtca
      601 gtacaaagtg aggctaccct atgcctgaac accataaggt tagcctctta ctcgttggaa
      661 atcaacacag ggggctggga atgccgcaga aatatagatt actttcttta atagtgattt
      721 gtttcacgct tttatttttc acctggatga taagagattc actgtgtgaa ttgcatatta
      781 aacaggagag ttatgagctg gcggcgtttt tagcctacaa attgaaagag taagagtctt
      841 cggcgggaaa ttattcccgc cttacttacg gcgttgcgca ttctcattgc acccatattt
      901 attcttcaca aaaataataa tagattttat tacgcgatcg attatttatt tcctgaaaaa
      961 aaataaaaaa atccccgcca aatggcaggg atcttagatt ctgtgctttt aagcagagat
     1021 tacaggctgg ttacgttacc agctgccggg cctttagcgc cgctttcgat ggtgaaggac
     1081 actttctgac cttcgtccag agatttgtaa ccatcgttct ggatagcaga gaagtgtacg
     1141 aacacatctt tagagccatc gtcaggagtg atgaagccga agcctttgtc agcgttgaac
     1201 cattttacga taccagtcat tttaccggac atagtgtatt acctttaata attaagtgtg
     1261 cctttcggcg atatggcgtg ctttacagat tttgaagcgt taaaggaatg tgcactacga
     1321 ggggtatcaa cgataactct tgaagggact tgccttacta cactgcttta atggtctgta
     1381 cgtcaaaccg ttgatgtgca ttaagccact cattggcggg tgatgcaaca attatttttc
     1441 atatttatga ttaatcggtt ggaaaacggt cctgtcatca ggaccgtaaa cagcaataaa
     1501 gtggataaaa gtctatttca tcaactgctt acttaatgcc gggttggctt gaatcaaacg
     1561 catcaatttt agttcggcac ttgaaggctt cacgcgtctg gattcccatt cctttaccat
     1621 ggcgactgat acgcccaaaa cccgggcgaa atcatcgatt tttaatcctg tcccttttca
     1681 taactgctca atttcggtac aggacgttgt tctgtgcgtc agggtaattt tctgcgtttc
     1741 atctttaaaa acaatctgtt ccaggctgct caacagctca tgcattggat ctttatattc
     1801 cattgaaaac tcctcaaatc acactgcggg atcgtgaatt gcatcgaagc tcattaagaa
     1861 tagtcgggaa aagcagacac agcggggtga agtcagggca tgctctggct atgattcgat
     1921 tcagcctgtc atttagcgtg acttatagcc acctaacgat ctgataaatg ccgatatatc
     1981 tcttatcact cctgttttgt aaagatttcg caaatctaac cttctgcgaa ggcgtaaatc
     2041 tctggctaca aaaaaggcaa cggcactatc attttttaac aatgaaacaa caaattaacc
     2101 ttgtttcatt ttctccatga ttttaccagg aagattacgt agaaagggta tcctgcaagc
     2161 ctgtcctgga ctatccttgt cacgtcagac acgcgtgtgt cgttgtgcgc tttttttggg
     2221 tgaaaggagt aagaaaatgg cgacaggaaa gtcctgctct cgctggtttg cgcctcttgc
     2281 ggcgttatta atggtagtta gcctgagtgg gtgttttgat aaagaaggcg atcagcgtaa
     2341 agcgtttatc gacttcctgc aaaatacagt gatgcgtagc ggtgaacgtc taccaaccct
     2401 gactgccgat cagaaaaaac agtttggtcc ttttgtctct gattacgcga ttctgtatgg
     2461 ttattctcag caggtgaatc aggcgatgga ttccggtctg cgcccggtcg tagacagcgt
     2521 taacgcaatt cgcgtaccgc aggattacgt tacgcaaagc ggtccgctgc gtgaaatgaa
     2581 tggttcgctg ggtgtgctgg cgcaacaact gcaaaatgcg aagttgcagg ccgatgctgc
     2641 gcactctgcg ctaaaacaga gcgatgacct gaaaccggta tttgatcagg cattcaccaa
     2701 agtggtaacg acgccagctg atgcattgca accgttgatt ccggcggcac aaactttcac
     2761 gcaacaactg gtgatggttg gagactacat tgctcagcaa ggtactcagg tgagctttgt
     2821 cgcaaatggc atccagttcc cgacttcaca gcaggcaagt gaatataaca aactgattgc
     2881 gccattaccg gcacaacatc aggcgtttaa tcaggcctgg actacagcag ttactgcaac
     2941 ccaataaaga gtaaaagccc gagcgattat cgggctttac ttcacgcagt cgcagcttag
     3001 tccgcgacgt gaggattcac acagttcttc tcaacctttc cttgtaacgc atcaatcaaa
     3061 ttatccacgg cacaggcggc catgccataa cgcgtctcat gggtggcaga tccaatatgc
     3121 ggtactgcga cgacgttggc cattgagagc aacggcgaat ctacggacag tggctcttgt
     3181 tcgaagacat ccagcccggc ggcgtgaatt tcgcctttct gcaatgctgc gatcagtgca
     3241 ttttcgtcaa ccaccgggcc acgtccggca ttaatgaaaa tggcggagga tttcattttg
     3301 gcgaattgtt ctgcgccaaa cagatgatgc gtctcatcag ttaacggcag gatcaggcaa
     3361 acgaaatctg actcttgtaa cagagtatcc aaatcgcagt agcgggcgtt gaagcgttct
     3421 tctgcttctt tatggtggcg gcgcgcgtta tagaggatgg gcatgttgaa gccaaagtgc
     3481 acacgttgtg ccagcgccat gccgatccgt cccatcccga caatgcccag tgttttatgg
     3541 tgaacgtcag tgccgtacca gtccgggcct atgctcgcgg tccattcgcc tgcttttacc
     3601 cgttctgcta cctccacaac ccgacgagcg gtagacaaca ccagcgccat cagcgtatcg
     3661 gcgacggttt ctgttaatac ggttggcgtg tgcatcagca gaatttttcg ggcggtaagc
     3721 gcatcgacat caaaattgtc atagccgacg gagatcgttg atgtggcacg cagtttcggc
     3781 attttttcca gcaatgcggc atctacattc tcgtttgaac ccagtaaacc ttcagcttct
     3841 gcaaaaattg ctgcattttg aacgacggtt tgtgggctga ggtttgccac ctggtgaacg
     3901 gtgaaatgcg cttgcaggcg ttgcagtaaa tcatcaggta aggctttgta gaggataacg
     3961 gacggcttca tgcttctctc cattcactga taaggcgatc accttagcct gaaatatgat
     4021 gaaggaaaac tgaagaatgc caggtgattt ctgcatcacc tggcatgaaa gggattacag
     4081 cgggcttaag gtaatttcta cacggcggtt ttgcgcctta ccttctgcgg tgctgttgct
     4141 ggcgattggg ttagccgggc caaggccctg agtacggatg cggctggcgt ccacgccctg
     4201 ggtgatcaac gcgctggcaa cggaatccgc acgctgctgg gagagacgca tgttcaggtc
     4261 gtgacccccc gtgctgtcgg tataaccaat cacgttaacc gccgttttcg gatactcttt
     4321 cagcaccatt gccacgccgg tcagggtgtt agcgcctgcc ggtttcaggg gagcgctgct
     4381 gctgtcgaag gtcacattgt tcggcatatt gaggataatg ttatccccgc tgcgggttac
     4441 gctaacacca gtgccgcgca ttttgtcacg cagcttcgct tcctgcacat ccatgtaata
     4501 acccacgccg ccgcccagag ctgcgcctgc tgctgcgcca atcagcgcgc ctttaccgcg
     4561 atctttcttc gaagaagaga gcgcaccaat acccgcgccc acgagagagc ccagacctgc
     4621 gccgatagca gatttgcctg cttcgcgttc gccggtgtaa gggttagttg tgcagccaga
     4681 taccgccaga gcgccactca ctacggcggc aagtaagata aacacgtttc ttcattgtta
     4741 atccttaata acctttttat tctttgtcac gggttccgtg gcgggagatt atgccgcgtg
     4801 aacatgaaga ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat
     4861 acatctaacc tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg
     4921 actgccgccc actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg
     4981 cgtggcgcgt tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt
     5041 cacagcaata cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa
     5101 aacccgcaag gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg
     5161 gatcttattc accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt
     5221 gctggttcct acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa
     5281 cgctatatgg cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg
     5341 gcacaggcga tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt
     5401 tggccgctgg tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat
     5461 acgctgaaaa ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg
     5521 cgtgacagtg ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc
     5581 tttggcgata aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg
     5641 gggatcgccc atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc
     5701 accacaggtt atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa
     5761 aacgccgaat gggcggcaga gatttgtggt gttggcgcag cgaaaatccg cgagctggcg
     5821 gctattttcc accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag
     5881 tttggtgagc aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc
     5941 acacccggcg gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg
     6001 cgttctgcgg tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat
     6061 aaaatccctg ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac
     6121 aacggtatga accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac
     6181 tttactcatc atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg
     6241 gtgatctctg aatgcttctg gacggcggcg gcgaaacacg cggatattgt tctgcctgcg
     6301 actacctctt ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg
     6361 gtgccgatga agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt
     6421 gccgagttga gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt
     6481 cagctgcaat ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag
     6541 gttgaattgc cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa
     6601 aacccggaca gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat
     6661 ccgttaaaaa ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt
     6721 tacccggatt gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc
     6781 gaaccagaac agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg
     6841 aattacagtt ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat
     6901 cctgacgatg ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc
     6961 cgtgggcaga tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc
     7021 attcacgaag gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc
     7081 gcagtgaacg tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt
     7141 aatacgacgc tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt
     7201 gaaccaccgg ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat
     7261 cgacaatgtg aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa
     7321 cctccagcat cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac
     7381 cgcgtctgac ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc
     7441 tgacaaaacc gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat
     7501 aggcatcccg caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc
     7561 cccaggttgt actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt
     7621 cccgaatcat ggtttatttc ccagatagca acagcagcca accacatgat cattcaccag
     7681 cccacatgcc tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt
     7741 tttcagtgcc ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt
     7801 tgtggcttgt gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cgggaaacgg
     7861 ttcgccgttc tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt
     7921 ccctcgatgg cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat
     7981 tgctgcgacc ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt
     8041 gaggacggtg atccacgata acccagcctg ctgcccttca aagcagatca tttcgaacag
     8101 ttttttactg tcagtttcag gcacgcccca ctcattatca tggtaggcaa tataaagcgg
     8161 gccctgactc acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa
     8221 aatttcagcc gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact
     8281 atgcccgata tccttcatac aatgacaata atattgccga attcggctct tccctggagt
     8341 cgtgttaatg atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt
     8401 ggtaatcagt gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga
     8461 tacgcaaccc ggacattcca cagagcgtta cacctgggat agtgatcatc aacctgatta
     8521 caacgatatt ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa
     8581 tctggcggaa gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc
     8641 tttatatgaa caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac
     8701 ctcgatgtat aacgagtttg gcgacagtac taccacgctg accgatccgt tatggcatgc
     8761 cagcgtgagt agcttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc
     8821 gcaaatcagc tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag
     8881 ccggatgacg gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat
     8941 gttgctcaat caaaatattg ccgcctatgc tgcgctaact caggcagaaa ataccactaa
     9001 taatagcgac tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa
     9061 ttgaaaccat tgaataacaa cagttgtggc gatagtgggt gctaacttac caaataataa
     9121 atttggtgaa taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga
     9181 tggcatttca tgccgttttt ccccaagcat aaaatgcact tcgttatggt tgtcggcaga
     9241 gatttttcct ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac
     9301 ccgctggctg acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac
     9361 ctggagcctg tttaatggcg cgctttctgc caagctggat gcgccggtaa gccaggtcgc
     9421 tttctctttc ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt
     9481 gcaggaacgt tttggcgtta aacgcgtcac cgtggcttcc ggcattttgc tgggattagg
     9541 cttcttcctg accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct
     9601 ggtcggtctg gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt
     9661 cccggagcgt aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct
     9721 gggtttcaaa tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacctttgt
     9781 gatttgggga gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc
     9841 accaaaacag gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga
     9901 gtcgatgcgt aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag
     9961 cggcctgtac gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt
    10021 ggtttccgca gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt
    10081 gctgggcatt ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat
    10141 ttcgctggtg ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc
    10201 agcgattgcc tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt
    10261 cagtgagttc ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt
    10321 cggtatcggt agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac
    10381 cttctacgtg attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca
    10441 gccagagcag aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta
    10501 ctgggcttca gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt
    10561 aaaatttggg ccagttaaca ggcccttttt actgcttttt gtaacgctaa agtctctttt
    10621 caaacttgca tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct
    10681 ttgctgtggt ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt
    10741 tcccctgccg ggcctgaaaa gccgctaagc agggtgttat cacctgtttg tccagggttt
    10801 gtttgcatga gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc
    10861 tatattggcc tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac
    10921 gattttaccg tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg
    10981 accttctttt tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg
    11041 tcgctggtgg tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc
    11101 attggttatg gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt
    11161 gttggtctga actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc
    11221 tggaataacc gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt
    11281 cgcagcctgg tggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg
    11341 gatatccagc agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt
    11401 gtcgtagcga actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg
    11461 gcgcgggtcg atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca
    11521 taccaggctc cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg
    11581 cgttgggacc atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag
    11641 gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg
    11701 cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa
    11761 cagaacattt tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag
    11821 agcgaaatgt ggttctacag caacacgatg gcagacaaca ttgcttatcg tgagcagatt
    11881 ggtgcggagc cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg
    11941 cagcaatcgc tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt
    12001 tcgcatttta actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt
    12061 attggtgttg atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg
    12121 acctatgtgg atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt
    12181 gtgttctacg cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg
    12241 ccgcgtgaac tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat
    12301 aaatatctgg aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac
    12361 atgaaagtgc cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat
    12421 acttcaccgg atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa
    12481 gtggcagcgg ctaactaagc ggcctgctga ctttctcgcc gatcaaaagg cattttgcta
    12541 ttaagggatt gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc
    12601 gcagcttggt agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac
    12661 cgaccaaatt cgaaaagcct gctcaacgag caggtttttt tgcatttgta gctcatgagg
    12721 atgagaacct ccgggggcag ggaggctcga ctcgacgtag gcctgataag acgcgccagc
    12781 gtcgcatcag gcaacggcac cgcacttatt gtcggatgcg gcgcgaccgc cttattcgac
    12841 caacacacca cacattttcc tgccaacttt gcgccccata tctcactatc cattaacgga
    12901 tttgtgacag aaaccattgt ctttttttat atttgcttaa atcttttttg cgttacttat
    12961 ggctggcgct ggcattttcc gctgtgcgtt ttagcgttca tggtattaat cgctcagata
    13021 tccacctttt cctcagattt ttttcattaa atgcataaaa cttaatcacc aaatgttgct
    13081 aaatattaag ctgattgttc tggttatgtt ggagtagcac aaatttctct gctgcaaata
    13141 gctgtttgaa gtttttttaa tctttgtttg tggattctca ccgttggtgt aaatcccggt
    13201 tggctgtatt gacgttttca cattctgttg acagattgta ggtcacgagg ggcattttat
    13261 ggaggatccg cactgttaca ctgatgttaa ttagtacggc atccccacct cataacgttg
    13321 acccgaccgg gcaaaaaaca aaaaaggtca ggcagcgaca acccactgca aagggttaaa
    13381 acaacaaaca tcacaattgg agcagaataa tgcgtatttc cttgaaaaag tcagggatgc
    13441 tgaagcttgg tctcagcctg gtggctatga ccgtcgcagc aagtgttcag gctaaaactc
    13501 tggtttattg ctcagaagga tctccggaag ggtttaaccc gcagctgttt acctccggca
    13561 ccacctatga cgcctcttcc gtcccgcttt ataaccgcct ggttgaattt aaaatcggca
    13621 ccaccgaagt gatcccgggc ctcgctgaaa agtgggaagt cagcgaagac ggtaaaacct
    13681 ataccttcca tctgcgtaaa ggtgtgaagt ggcacgacaa taaagaattc aaaccgacgc
    13741 gcgaaatgaa cgccgatgac gtggtgttct cgttcgatcg tcagaaaaac gcgcaaaacc
    13801 cgtaccataa agtttctggc ggcagctacg aatacttcga aggtatgggc ttgccggagc
    13861 tgatcagcga agtgaaaaag gtggacgata acaccgttca gtttgtgctg actcgaccgg
    13921 aagcgccgtt cctcgctgac ctggcaatgg acttcgcctc tattctgtca aaagaatatg
    13981 ctgacgcgat gatgaaagcc ggtacaccgg aaaaactgga cctcaaccca atcggaaccg
    14041 gtccgttcca gttacagcag taccaaaaag attcccgtat ccgctacaaa gcgtttgatg
    14101 gctactgggg caccaaaccg cagatcgata cgctggtctt ctctattacc cctgatgctt
    14161 ccgtgcgtta cgcgaaattg cagaagaatg aatgccaggt gatgccgtac ccgaacccgg
    14221 cagacatcgc tcgcatgaag caggataaat ccatcaacct gatggaaatg ccggggctga
    14281 acgtcggcta cctctcgtat aacgtgcaga aaaaaccgct ggatgacgtg aaagttcgcc
    14341 aagcactaac ctacgcggtg aacaaagacg cgatcatcaa agcggtttat cagggcgcgg
    14401 gtgtatcagc gaaaaacctg atcccgccaa ccatgtgggg ctataacgac gacgttcagg
    14461 attacaccta cgatcctgaa aaagcgaaag ccttgctgaa agaagcgggt ctggaaaaag
    14521 gtttctccat cgacctgtgg gcgatgccgg tacaacgtcc gtataacccg aacgctcgcc
    14581 gcatggcgga gatgattcag gcagactggg cgaaagtggg cgtgcaggcc aaaatcgtca
    14641 cctacgaatg gggtgagtac ctcaagcgtg cgaaagatgg cgaacatcag acggtaatga
    14701 tgggctggac tggcgataac ggggacccgg ataacttctt cgccaccctg ttcagctgcg
    14761 ccgcctctga acaaggttct aactactcaa aatggtgcta caaaccgttt gaagatctga
    14821 ttcaaccggc gcgtgctacc gacgaccata acaaacgcgt tgaactgtac aagcaagcgc
    14881 aggtggtgat gcacgatcag gctccggcac tgatcatcgc tcactccacc gtgtttgaac
    14941 cggtacgcaa agaagtcaaa ggctatgtgg ttgatccatt aggcaaacat cacttcgaaa
    15001 acgtctctat cgaataatta aaagccatac aagactgatg gcaaaggcaa aactgcctga
    15061 tgtgctccgc ttatcaggcc tacgaaaatt ctgcaatgta ttgaatttgc acgattttgt
    15121 aggccggata aggcgttaac gccgcatccg gcataaacaa agcgcacttt gtcaacaatc
    15181 tgtatacccg gtggcgctgt gcctctgcgt gcagcgccat ccggcagcaa tacttcattc
    15241 tctgtccgac aagggcggga attgatttgt gagcaataca gacacgcagt tccaggctgc
    15301 gagtcactat agagaatccg ggttatgttg cagtttattc tccgacgttt gggactcgtc
    15361 atccccacgt ttatcggtat tacccttctc acatttgcct ttgtccacat gatcccgggc
    15421 gatccggtga tgatcatggc aggcgaacgt gggatctccc ctgagcgtca cgtgcagctg
    15481 ctggctgaac tcggcttaga taaaccgatg tggcagcagt atctccatta catttggggc
    15541 gtaatgcacg gcgatctagg catttcaatg aaaagccgaa ttccagtatg ggaagagttc
    15601 gtgccgcgct ttcaggccac gctggaactt ggcgtctgcg cgatgatttt tgctacggca
    15661 gtcggtattc cggttggtgt gctggctgcg gttaaacgcg gttccatttt cgatcacact
    15721 gcagttggcc tggcgctgac cggctactcg atgccgatct tctggtgggg catgatgctg
    15781 atcatgctgg tttcggtgca ctggaacctg acgcccgtct ccggtcgcgt gagcgatatg
    15841 gtgttcctcg atgactccaa tccgttaacc ggttttatgc taatcgacac cgccatctgg
    15901 ggtgaagacg gcaactttat cgatgccgtc gcccatatga tcttgcccgc cattgtgctg
    15961 ggtactattc cgctagcggt cattgtgcgt atgacacgct cctcgatgct ggaagtgctg
    16021 ggcgaggatt acatccgcac cgcgcgcgcc aaagggctaa cccgcatgcg ggtgattatc
    16081 gtccatgcgc tgcgtaacgc gatgctgccg gtagtgaccg ttatcggcct ccaggtggga
    16141 acattgctgg cgggggcgat tctgaccgaa accatcttct cgtggcccgg tctggggcgc
    16201 tggttgattg acgcactgca acgccgcgac tatccggtag tgcagggcgg cgtattgctg
    16261 gtggcgacga tgattatcct cgtcaacttg ctggtcgatc tgctgtacgg cgtggtgaac
    16321 ccgcgtattc gtcataagaa gtaaggggac atcatgtcac aggttactga aaataaagtg
    16381 attagcgcac cggtgccgat gaccccgtta caggagttct ggcactattt taaacgcaac
    16441 aaaggcgcgg tcgtcgggct ggtttacgtc gccatcgtgc tgttcatcgc gatctttgcc
    16501 aactggattg cgccttataa cccggcggat cagttccgcg atgcgctgct cgccccgcca
    16561 gcctggcagg aaggcggcag tatggcacac ttgctgggca ccgatgacgt cggtcgtgat
    16621 gtgctgtcgc ggctgatgta cggcgcgcgt ttatcgctgc tggttggctg tctggtggtg
    16681 gtgttgtcgc tgattatggg cgttattctc ggcctgatcg ccggttactt cggcggcctg
    16741 gttgataaca ttatcatgcg cgtggtcgat atcatgctgg cgctgccaag cctgctgttg
    16801 gcactggtgc tggtggcgat tttcggcccg tcgattggta acgccgcgct ggcgctgacc
    16861 ttcgttgcct tgccgcacta tgtgcgctta acccgcgccg ccgtgctggt ggaggtgaat
    16921 cgcgattacg tcaccgcctc tcgcgtggca ggtgccgggg cgatgcgcca gatgtttatt
    16981 aacatcttcc cgaactgcct tgcgccgctg attgttcagg cgtcgctcgg tttctctaac
    17041 gccattctcg atatggctgc tcttggtttc ctcggcatgg gggcacagcc gccaacgcct
    17101 gagtggggca ctatgctctc cgacgtgttg cagttcgcgc aaagcgcctg gtgggtcgtg
    17161 accttccctg gtctggcgat cctgttgacg gtgctggcat ttaacctgat gggtgacggt
    17221 ctgcgtgacg cgctcgatcc caaactaaag cagtaagagg ttcgagatgg cgttattaaa
    17281 tgtagataaa ttatcggtgc atttcggcga cgaaagcgca ccgttccgcg ccgtagaccg
    17341 catcagctac agcgtaaaac agggcgaagt ggtcgggatt gtgggtgagt ccggctccgg
    17401 taagtcggtc agttcactgg cgattatggg gctgattgat tatccgggcc gcgtaatggc
    17461 agaaaaactg gagtttaacg gccaggattt gcagcgtatc tcagaaaaag agcgccgcaa
    17521 cctggtgggt gccgaagtgg cgatgatctt ccaggacccg atgaccagcc ttaacccgtg
    17581 ctacaccgtg ggtttccaga ttatggaagc gattaaagtg catcagggcg gcaacaaaag
    17641 tacccgccgt cagcgggcga ttgacctgct gaatcaggtc ggtattcccg atccggcttc
    17701 gcgtctggat gtttacccgc atcagctttc cggcggcatg agccagcgcg tgatgatcgc
    17761 catggcgatt gcctgtcggc caaaactgct gattgccgat gaaccgacca ccgcgctgga
    17821 cgtgaccatt caggcgcaaa tcatcgaact actgctggag ctacagcaga aagagaacat
    17881 ggcgctggtg ttaattaccc atgacctggc gctggtggcg gaagctgcgc ataaaatcat
    17941 tgtgatgtat gcaggtcagg tagtagaaac gggcgatgca cacgccatct tccatgcgcc
    18001 gcgtcacccg tatactcagg cattgctgcg tgcgctgcca gaatttgctc aggacaaaga
    18061 acgtctggcg tcgttgccag gtgttgttcc cggcaagtat gaccgcccga acggctgcct
    18121 gcttaacccg cgctgcccct atgccactga cagatgtcgc gctgaagaac cggcgctgaa
    18181 tatgctcgct gatgggcgtc agtccaaatg ccattaccca cttgatgatg ccgggaggcc
    18241 cacactatga gtacgcaaga ggccacctcg caacaaccgc tgttgcaggc tatcgacctg
    18301 aaaaaacatt atccggtgaa gaaaggcatg ttcgtgccgg aacgtctggt gaaggcgctg
    18361 gacggcgttt cgtttaacct tgaacgtggc aaaacgctgg cagtagtggg cgaatctggc
    18421 tgcggtaaat cgaccctcgg tcggttgctg acgatgatcg aaacgcccac cggcggcgag
    18481 ctgtattatc aggggcagga tctgctcaag cacgatccgc aggcgcagaa gctgcgtcgg
    18541 cagaaaatcc agatcgtctt ccagaatccc tacggttcgc tgaatccgcg taagaaagtt
    18601 gggcaaattc tcgaagagcc gctgcttatt aacaccagct taagcaaaga acagcgtcgg
    18661 gaaaaagccc tgtcgatgat ggcaaaagtc ggcctgaaaa ccgagcacta tgaccgctat
    18721 ccgcacatgt tttccggcgg acaacgtcag cgtatcgcta ttgcccgtgg tctgatgctc
    18781 gacccggatg tggtgattgc cgatgagccg gtttctgcgc tggacgtctc agtgcgtgcg
    18841 caggtgctga atctgatgat ggatttgcag caggagttgg ggctttctta tgtctttatc
    18901 tcccacgacc tgtcggttgt ggagcacatt gctgatgaag tgatggtgat gtacctaggc
    18961 cgctgcgtgg agaaaggaac gaaggaccaa atcttcaata acccgcgcca tccgtacacc
    19021 caggcgctgc tctccgcgac gccacgcctg aacccggacg atcgtcgtga acgcatcaaa
    19081 ctcaccggtg aactgccaag cccgctgaat cctccgccgg gttgcgcctt caacgcccgc
    19141 tgtcgtcggc gcttcggccc ctgcacccag ttgcagccgc agctaaaaga ctacggaggt
    19201 caactggtag cttgttttgc tgttgatcag gatgaaaatc cgcagcgtta actcacaaaa
    19261 ccggggccat gcgctccggt ttactcaatc agcttcaaca gtggcgacaa gcatagcaaa
    19321 atgccgtaaa gcagaatcag ccaggcttta aatccgcgaa gtttttccag ttgtgcgact
    19381 ttatagatca ggaaaaacgg aatgaggcac gacacaatgc catataacgg gcttcccaac
    19441 tgaaagaaca ccagcactga tacacgaaac gaaacccaaa tcgtcaacgt aatgacgata
    19501 aaagcgcaga tcgccagagt cagcatgcgt gagttaattt tcttggtatc aataattcgg
    19561 cttaacagat tgagaataat gcctttaata gcctcgtgga aaccgagata aatgccaaag
    19621 aatgcggtca gtacggcaaa gatattcagc accgtagagg tgatatgaat gatatgtcca
    19681 gggatcacct gcgcggccag tgccagtgcc gagatatttt gttcaaaggc agaaacggct
    19741 tcttcgtggc taattgagaa ggtaaacgaa aaggcaaaaa acaggatcac cgcgatgagc
    19801 gtgatataac taattcggtg ggtacgcagc gcgagccggg ttgccagtac tttatctgct
    19861 tcccgtttac gataggcaat attcattggg ttaagtacct gaataaatac cgcagaaaag
    19921 aagcaaaatg gaatggtaag caggacatcg cggaaaaaga cggaggcttg cgggaaggcg
    19981 gttatattgg cgaaattcca gtgcgggatc atcgcaaaac cgaacacgac aataatccct
    20041 actttgacca ccaccattgg cccagaaatc ttaaacagta atcgttcacc accagacgca
    20101 atcgctatca gtacggcgaa aatagcgact ttataaagta gagattgtga aagatcggca
    20161 tcggttaaac cgaaggtttt caggtacgag gcgctgtcga aaaccacgga gagagagtag
    20221 ataaaaatcc cgtggataat catcaaaaag tagataaccc cgaggaaaat tccccagttc
    20281 ttccccaggt aatgactgat aatatcggtg tagtcattac aggaatcgct ttcagaaagg
    20341 gtttttaaat aaatgtcctg cactacccag gtgacaggat aagcaatgat cgctgcggta
    20401 ataaataccc aaattccctt caagccaatt tgtactggca tcagcacggt tccggcacca
    20461 atcgccatgc ctatgcataa taaaacccag ccgaagtcgt agcgtgtaaa cggcaatttc
    20521 tgattgtgtt tcgatagtgt gttgtgctgc ataaagtcct gtccagaaag aagtcggcct
    20581 cgcaaaaatg aataaaccca gtttctcttg cgcaacaggt ggagaagtcg aatacgcagt
    20641 atgtaaaaaa tgaggcaaca gattcactgg ggaggtcgcg aggaggcgca attctacaaa
    20701 attgcgcatt tcactaccag tctggagtca gcccggatat gaactgcaac tattgcaaat
    20761 aaagaaatta agctggaagg catcgcgcaa tgaggaagat tgccgggtgc tgacagtaaa
    20821 aagcaatcgt agaatcagcg tcaagccgga aggattccgg tgaggcgcaa tgttgcgggg
    20881 gcttaatccc tggtggcatt ggttgctgga gagagaaacc cccgcacgtt gcaggtatgt
    20941 acctgacaac ataacggggg ctaatcttga acctagacca ctcaagaata gccgcgaaac
    21001 gttgtcatta caacacaggc ggctatatga cgctcgcaga gctgggcatg gccttctggc
    21061 atgatttagc ggctccggtc attgctggca ttcttgccag tatgatcgtg aactggctga
    21121 acaagcggaa gtagcgtgtc atgtgggcgt caggctgccg taatggcaat ttgcgcccgg
    21181 accaggccgc aggggtgaaa ctctgcggcc tttttcgtaa gaaaaatgca acggttgcaa
    21241 gcggataata ttctgcggaa tgcgcagcac agagtgaaga tgagacggat tccggaacag
    21301 aaaaacagtc gtagaatcag cgtcaagccg gaaggattcc ggtgaggcgc aatgttgcgg
    21361 gggcttaatc cctggtggca ttggttgctg gagagagaaa acccccgcac gttgcaggta
    21421 tgcacctgac aacaccacgg gggccattct tgcatccaaa caatacaaga atagccgcga
    21481 aacgttgtca ttacaataca ggcggctata tgacgttcgc agaactgggc atggccttct
    21541 ggcatgattt agcggctccg gtcattgctg gtattcttgc cagtatgatc gtgaactggc
    21601 tgaacaagcg gaagtaacgt gccatgcggg cgtcaggctg ccgtaatggc aatttgcgcc
    21661 cggaccaggc cgcaggggtg aaactctgcg gcctttttcg taagaaaaat gcaacggttg
    21721 caagcggata atattctgcg gaatgcgcag cacagagtga agacgtgacg gattccggaa
    21781 cagaaaaaca gtcgtagaat cagcgtcaag ccggaagggt tccggtgagg cgcaatgttg
    21841 cgggggcttt atccctggtg gcattggttg ctggagagag aaaacccccg cacgttgcag
    21901 gtatgcacct gacaacatca cgggggccat tcttgaacct gaacaactca agaatagccg
    21961 cgaaacgttg tcattacaac acaggcggct atatgacgct cgcagaactg ggcatggcct
    22021 tctggcatga tttagcggct ccggtcattg ctggcattct tgccagtatg atcgtgaact
    22081 ggctgaacaa gcggaagtaa cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc
    22141 gcccggacca ggccgcaggg gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga
    22201 ttgcacgcgg ataatattct gcggaatgcg cagcacagag tgaagacgtg acggattccg
    22261 gaacagaaaa acagtcgtag aatcagcggc aagccggaaa ggttccggtg aggcgcaatg
    22321 ttgcgggggc tttatccctg gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg
    22381 caggtatgca cctgacaaca tcacgggggc taatcttgaa cccaaacaac tcaagaatag
    22441 ccgcgaaacg ttggcattac aacacaggcg gctatatgac gctcgcagaa ctgggcatgg
    22501 ccttctggca tgatttagcg gctccggtca ttgctggcat tcttgccagt atgatcgtga
    22561 actggctgaa taagcggaag taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt
    22621 tgcgcccgga tcaggccgca gaggttaaac tctgcggcct ttttcgtgct tactgcgggt
    22681 aaggcaccca gtcgccgccg ttcaggcgaa cgtacggttt atcttggtat tgaataacta
    22741 ctgcatttga gttctcggag accggtgctg tttgtggcaa cccactggtg agttttttcc
    22801 agtcaacatt gtcttcggtg aaaatcttgc catcaagaac gcgaaccacc agatcggaga
    22861 tagccaggaa gctgctcggt tggtcaatga caatcggtgc cccctgatgt ggtgccttca
    22921 tgccgaagaa tttcacccca acggggacgt ctgtgataga cgggctaggg atatcacgta
    22981 ggccagatac ctgcattctg tcgcccttca gcgcgccgcc gtgttccggc accacgacca
    23041 ccatcacttt acgacccgat ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga
    23101 atttctgcgc ccgcgctttg taatccgctg ttttgctgac ccccggataa tggttgccgt
    23161 catgcagtgg aagcgtgttg tagaacgtgg cactacggct gtttttatct ttttcggtaa
    23221 cgtccagcca gcggttaagc acggcggtat cgtcataaac cggcgaacca tcaaagccca
    23281 gcaaaataac cggcagattt gtttgatcca tcaattcagt ctgcatgccg ccattttcgc
    23341 gaacttcttt caaaaaaccg ccgaactggc cgttatgtcc catcatcagg tgctgggtaa
    23401 agcccagttt cgaaaggtta tcaaacagat agcagtcgtt atttgccggt tgatacagat
    23461 tagtgtgcga agtctgcccg cagctggcgc gcagtaaacg gatcgccgcc gggccactgt
    23521 aggaggtggc ggagttaaag ttcttgaaat caatatcgaa atgcgaccac agtggatgcg
    23581 acatcaaccc ggcggcttct atatccgacc aggaaagcga acagatgtta atcaccagta
    23641 gttcaaatgg ctgagcatca gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct
    23701 ccgcgttata gaaattattc agccaggcgt taaggttcgc cgttgttggc ggtgcagttt
    23761 gtgcgggcat atcacccact accggtgcgc cacccgtcgc cgcaacggtt gccgctgcgt
    23821 taccacccgt cgttgttaca gtggtcgtcg gttgtccggc tggccacaag gagaaacttg
    23881 gtcccgccaa ggtaagtacg ttcagccata gcagtatggc aaccacaaaa acggtaatgc
    23941 gaatccattg tgacaggaat aaccaggcca ctaataaaac aaaaatggcc ccaatcatct
    24001 gccagttaat aaagcgtgtg acaaggtcga ttaaataatc ggtactgaac cccgccacct
    24061 gcgaaccctg gctcattatg ctttccgggc caggcaacca gatgtcatgc cagaacaaag
    24121 caaagccgat cggcagggca atccagtggc gcaagcgatg caggctgtaa cgcggaatgg
    24181 gcatcagcag aaacgcggca aacaccaaat tgaggagcgg gtggaagtta agatatcccg
    24241 cccacaacag gccaaactta accagaaaat agaagttcca gccggaaagg ccgcgccagt
    24301 attgccagag ggaagaaggc atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc
    24361 cctggctttt tccgtgcggc gtaacgtccc ggccggttta acataacgag gtttagcaaa
    24421 gaacaaacgt aaggtgtcgc gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg
    24481 gaaaaatatc agtgcgcaaa cgataataat ttcaatgata tcgctgatgg tcatcatgat
    24541 gagcgctcca cagcatcatc taacagtcgc atgggttctg gtattcgtcg ccagtggcga
    24601 ccatcgtgct cggcattgat gaccggttta gaactttgcg ttaaaggcag cggcattccc
    24661 cattgttctg gggcaagcag gcgcatctgc accagctcgg cactgatttg atcatcttca
    24721 aaccagacca tacggtttga gaaaatgtcg ccagtaggca atgggaaaat atgattcaac
    24781 gcggtatcca gatcgttaat ccgacagaat gagagaaaca gcaccagccg attaccgcca
    24841 atggtcatga tatcgccggt acggttaggg cgacatagcg tcagggcttg ctcaacgcgg
    24901 atacccggta ccggacgtag ggcaaccaga acgcctttac cgtgggcagg caagagaggg
    24961 ttattcatca tgttgttgac ggcattacag aacacatccc acttctggaa accacgcagt
    25021 ttgagcggct gggtcattga cagcaaggta gtgatatctt ccggtacata gcgactaaac
    25081 ttctgccctt gcacgctttc gatcatcgtc agacaacggg agagtggcgc attccacgga
    25141 ataaccatat ttgcaccgca ggccaataac aaacgttcat cggtggcgcg caggctagcg
    25201 gtattttccc gcacgaggat tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg
    25261 ctgcgggcca gtggctcgat ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc
    25321 tgagcggtac gggcttcatt gaacaggact tcgttattgt tgaacagttg ccagtgttcc
    25381 gatagcggcg gcgcaccttc cagtacagca acattactca gaatgcgttt ttcgtcgctg
    25441 cgtggttgga tctccgcctc ttcgctttga actaatgtcc agataccatt ttgttgctga
    25501 acgctaagct gctgacgggc gctgacccct ttttcgttgc accagaaggc aatatccagc
    25561 aaatgttggt caccctgaaa acgcaaactg gcaagaccaa aaagtgaacg gtattcctca
    25621 agcaataatg aaaattgttt atcgttatta tttccgggat taattaccaa aagcgaacaa
    25681 tggtttaacc tgctccattt attcatttta tccaaccatg agcgaagccg ctcggcagga
    25741 atattttgcc atgcgttatt tgcgcaaaca agaataaaaa ggtaattatg gggatcaata
    25801 gaacactgca aatcacgggc caaatagtat agaccttttt catgatttag cattgaaaat
    25861 aattttattt tttccggccc ttgagaatca tctaattgaa agattttcgc cggatcgctg
    25921 tccatgctaa tgaccgcgac gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc
    25981 agactgatag catcttcatg gcgatcgacg ttaaaccacc agacgccgcc tgctggcata
    26041 tgtcgcagct catcccataa tgatgagata ccgatagaga atacagggtc cacaatgtcc
    26101 ctcatgtcac cattttgtct gattatcagt ttactagcga aagctaagaa ataaacctac
    26161 cattgaaatt aaaacttatc agatttagca tgtgaatcaa tttagaaggt cacaagacgt
    26221 gtgattttag tcttttcgtt tttaactttt tctgttgcgt aacggaaagt caaaaagtga
    26281 gcaaattccc gtctcgccgt aaacaataaa ccggcgaaat gctcacggtt agaattgtct
    26341 catgaatggt acggttattt catagggatc aagcaaaatg aataacaatg aaccagatac
    26401 tctgcctgat cccgcgatag gctatatctt ccagaatgat attgtggcgt taaagcaggc
    26461 attttcactg cctgatattg attatgccga tatttcccaa cgcgaacagt tggccgcggc
    26521 attaaaacgc tggccgttgc tggcagagtt tgcgcaacaa aagtagggga ttggtgaatg
    26581 gccgtactgg gattgcaggg ggtgcgggga ggcgtgggga caacaaccat caccgccgca
    26641 ttagcctggt cattacaaat gttgggagaa aatgtcctgg tggtcgatgc ctgcccggac
    26701 aacttgttgc gcctgtcatt taatgttgat tttacccacc gtcagggctg ggccagagcg
    26761 atgctggatg gccaggactg gcgtgacgct gggttgtgct acacctcgca gctcgatttg
    26821 ctgccttttg gtcagttatc cattgaagaa caagaaaatc cacagcactg gcaaacccgg
    26881 ctgagcgata tttgctccgg cttacagcaa ctaaaagcca gcgggcgtta ccagtggatt
    26941 ttaatcgact taccgcgtga tgcctcgcag ataacccacc agctgctgag tttgtgcgag
    27001 cactcgctgg caatcgtcaa tgtggatgcc aactgccata tccgactgca tcagcaagcg
    27061 ctgccggatg gcgcacatat tttgattaat gacttccgta ttggcagtca ggttcaggac
    27121 gatatttacc agctttggtt gcaaagccag cgccgattac tgccgatgct cattcatcgt
    27181 gatgaagcga tggctgaatg cctggcggct aagcaaccag taggtgaata tcgcagtgat
    27241 gcgctggcgg ctgaagagat actgacgctg gcgaactggt gcctgttgaa ctactccggg
    27301 ctgaaaacgc cagtcgggag tgcatcatga gtatcctgac ccggtggttg cttatcccgc
    27361 cggtcaacgc gcggcttatc gggcgttatc gcgattatcg tcgtcacggt gcgtcggctt
    27421 tcagcgcgac gctcggctgt ttctggatga tcctggcctg gatttttatt ccgctggagc
    27481 acccgcgctg gcagcgtatt cgcgcagaac ataaaaacct gtatacgcat atcaacgcct
    27541 cgcgtccgcg tccgctggac ccggtccgtt atctcattca aacatgctgg ttattgatcg
    27601 gtgcatcgcg caaagaaacg ccgaaaccgc gcagcgggca ttttcaggtc tgcaaaatat
    27661 tcgtggacgt taccatcaat ggatgaacga gctgcctgag cgcgttagcc ataaaacaca
    27721 gcatctggat gaaaaaaaag agctcggtca tttgagtgcc ggggcgcggc ggttgatcct
    27781 cggtatcatc gtcaccttct cgctgattct ggcgttaatc tgcgttactc agccgtttaa
    27841 cccgctggcg cagtttatct tcctgatgct gctgtggggg gtagcgctga tcgtacggcg
    27901 gatgccgggg cgcttctcgg cgctaatgtt gattgtgctg tcgctgaccg tttcttgccg
    27961 ttatatctgg tggcgttaca cctctacgct gaactgggac gatccggtca gcctggtgtg
    28021 cgggcttatt ctgctcttcg ctgaaacgta cgcgtggatt gtgctggtgc tcggctactt
    28081 ccaggtagta tggccgctga atcgtcagcc ggtgccattg ccgaaggata tgtcgctgtg
    28141 gccgtcagtg gatatctttg tcccgactta caacgaagat ctcaatgtgg tgaaaaatac
    28201 catttacgcc tcgctgggta tcgactggcc gaaagacaaa ctgaacatct ggatcctcga
    28261 tgacggcggc agggaagagt ttcgccagtt tgcgcaaaac gtgggggtga aatatatcgc
    28321 ccgcaccact catgaacatg cgaaagcggg caacatcaac aatgcgctga aatatgccaa
    28381 aggcgagttc gtgtcgattt tcgactgcga ccacgtacca acgcgatcgt tcctgcaaat
    28441 gaccatgggc tggttcctga aagagaaaca gctggcgatg atgcagactc cacaccactt
    28501 cttctcgccg gacccgtttg aacgcaacct gggacgtttt cgtaaaacgc cgaacgaagg
    28561 cacgctgttc tatggtctgg tgcaggatgg caacgatatg tgggacgcca ctttcttctg
    28621 cggttcctgt gcggtgattc gtcgtaagcc gctggatgaa attggcggca ttgctgtcga
    28681 aaccgtgact gaagatgcgc atacttctct gcgtttgcac cgtcgtggct atacctccgc
    28741 gtatatgcgt attccgcagg cggcggggct ggcgaccgaa agtctgtcgg cgcatatcgg
    28801 tcagcgtatt cgctgggcgc gcgggatggt ataaatcttc cgtctcgata acccgctcac
    28861 cggtaaagga ctgaagtttg ctcagcggct gtgctacgtc aacgccatgt tccacttctt
    28921 gtcgggcatt ccacggctga tcttcctgac tgcgccgctg gcgttcctgc tgcttcatgc
    28981 ctacatcatc tatgcgccag cgttgatgat cgccctattc gtgctgccgc atatgatcca
    29041 tgccagcctg accaactcga agatccaggg caaatatcgc cactctttct ggagtgaaat
    29101 ctacgaaacg gtgctggcgt ggtatatcgc accaccgacg ctggtggcgc tgattaaccc
    29161 gcacaaaggc aaatttaacg tcaccgccaa aggtggactg gtggaagaag agtacgtcga
    29221 ctgggtgatc tcgcggccct acatcttcct tgttctactc aacctggtgg gcgttgcggt
    29281 aggcatctgg cgctacttct atggcccgcc aaccgagatg ctcaccgtgg tcgtcagtat
    29341 ggtgtgggtg ttctacaacc tgattgttct tggcggcgca gttgcggtat cggtagaaag
    29401 caaacaggta cgccgatcga accgcgtgga gatgacgatg cccgcggcaa ttgcccgcga
    29461 agatggtcac ctcttctcgt gtaccgttca ggatttctcc gacggtggtt tggggatcaa
    29521 gatcaacggt caggcgcaga ttctggaagg gcagaaagtg aatctgttgc ttaaacgcgg
    29581 tcagcaggaa tacgtcttcc cgacccaggt ggcgcgcgtg atgggtaatg aagttgggct
    29641 gaaattaatg ccgctcacca cccagcaaca tatcgatttt gtgcagtgta cgtttgcccg
    29701 tgcggataca tgggcgctct ggcaggacag ctacccggaa gataagccgc tggaaagtct
    29761 gctggatatt ctgaagctcg gcttccgtgg ctaccgccat ctggcggagt ttgcgccttc
    29821 ttcggtgaag ggcatattcc gtgtgctgac ttctctggtt tcctgggttg tatcgtttat
    29881 tccgcgccgc ccggagcgga gcgaaacggc acaaccatcg gatcaggctt tggctcaaca
    29941 atgatgataa cgcgatgaaa agaaaactat tctggatttg tgcagtggct atggggatga
    30001 gtgcgttccc ctctttcatg acgcaggcga cgccagcaac gcaaccactg atcaatgctg
    30061 agccagctgt agccgcccag acggaacaaa atccgcaggt ggggcaagtg atgtcaggcg
    30121 agcagggagc tgatgccccc atcgtggcgc agaacggtcc ttcgcgtgat gtgaagctga
    30181 cctttgcgca aatcgcgccg ccgccgggca gcatggtgct acgtggtatt aacccgaacg
    30241 gcagcattga gtttggtatg cgcagcgatg aagtggtgac gaaggcgatg ctcaacttcg
    30301 aatacacgcc atcgccatcg ttactgcctg tccagtcgca gttaaaggtt tatctcaatg
    30361 atgagctgat gggcgtgctg ccagtgacca aagaacagtt gggtaaaaaa acgctggcgc
    30421 aaatgcccat taatccactg tttattaccg acttcaaccg tgtgcggctg gagtttgtcg
    30481 gccattatca ggacgtgtgc gaaaacccgg ccagcaccac gctttggctg gatgttgggc
    30541 ggagcagtgg actggatctg acctatcaga ccctgaatgt gaagaatgac ctgtcacact
    30601 tcccggtgcc attctttgac ccgcgcgata accgtactaa caccttgccg atggtctttg
    30661 cgggtgcgcc ggatgttggg ctgcaacaag catctgccat tgtcgcctcg tggtttggtt
    30721 cgcgttctgg ctggcgtggg cagaacttcc cggtgctcta taaccaattg ccggatcgca
    30781 atgccattgt ctttgcaacc aacgacaaac ggccggactt cctgcgcgat catccggcgg
    30841 taaaagcccc ggtgattgag atgattaacc atccgcagaa tccttacgtc aaactgctgg
    30901 tggtgtttgg tcgtgacgac aaagacctgt tgcaggcagc gaaaggtatc gctcagggta
    30961 acattctgtt ccgtggtgaa agcgtggtag tgaatgaagt gaaaccgctg ctaccgcgta
    31021 agccgtacga tgcgccgaac tgggtacgta ccgatcgtcc ggtcacattt ggcgaactga
    31081 aaacctatga agaacagtta caatccagcg gccttgagcc agcagcgatt aacgtttcgc
    31141 taaacctgcc gccggatctc tacctgatgc gcagtaccgg cattgatatg gatattaatt
    31201 accgctacac catgccgccg gtgaaagaca gttcgcggat ggatatcagc ctgaataacc
    31261 agttcctgca atccttcaac ctgagcagca aacaggaggc gaaccgtctg ctgctgcgta
    31321 ttccggtatt acaaggtttg ctggatggca aaacagatgt ctctattccg gcgctgaaac
    31381 tgggcgcgac caaccagctg cgcttcgact ttgagtatat gaacccgatg ccaggcggtt
    31441 cggtagataa ctgtattacc ttccagccgg tgcagaatca tgtggtgatt ggtgacgatt
    31501 ccaccatcga cttctcgaag tattaccact tcatcccgat gccggatcta cgcgcctttg
    31561 ctaacgcggg cttcccattc agccggatgg cggatctgtc gcaaaccatc accgtgatgc
    31621 cgaaagcgcc taacgaagca cagatggaaa cgttgctgaa tactgttggt tttatcggcg
    31681 cacagacggg cttcccggcg attaatctga cggtgaccga tgatggcagc accattcagg
    31741 gcaaagatgc cgacatcatg atcatcggtg gtatcccgga caaactgaaa gacgataagc
    31801 agatcgacct attggtgcag gcgaccgaaa gctgggtgaa aacaccgatg cgccagaccc
    31861 cgttccccgg cattgtgccg gacgagagcg atcgcgcggc agaaacccag tcaactctga
    31921 cctcttccgg tgcgatggcg gcggtgattg gcttccagtc gccgtataac gaccagcgca
    31981 gcgtgattgc gctgttggca gatagcccac gcggttatga aatgcttaac gatgcggtga
    32041 acgatagcgg caaacgcgcc accatgttcg gttcggtcgc ggtgatccgc gagtccggta
    32101 tcaacagcct acgtgttggc gacgtttatt acgtaggtca tctgccgtgg ttcgagcgct
    32161 tgtggtatgc gctggcaaac catccgattc tgctggcggt gctggcgact atcagtgtga
    32221 tattgctggc atgggtactg tggcgtctgc tgcgaattat tagtagtcgt cgtcttaacc
    32281 cggataacga gtaattgaag atgaatgtgt tgcgtagtgg aatcgtgacg atgctgttgt
    32341 tggctgcctt tagtgttcag gcagcctgta cctggcctgc ctgggagcag tttaaaaagg
    32401 attacatcag tcaggaaggg cgcgtcatcg accccagcga cgcgcgcaaa atcaccacct
    32461 ccgaagggca aagttacggt atgttctttg ccctggcggc taacgaccgt gcagctttcg
    32521 ataatattct cgactggacg cagaacaatc tcgctcaggg ttctttaaaa gaacgtttgc
    32581 ccgcctggct gtggggcaag aaagagaaca gtaagtggga agtgctggac agcaattcgg
    32641 cctccgatgg tgatgtctgg atggcctggt cgttgctgga ggcggggcgt ttgtggaaag
    32701 agcagcgtta taccgacatc ggcagcgcgt tgctaaaacg tatcgcgcgg gaggaagtgg
    32761 tgacggtgcc tgggctgggt tccatgttgt taccgggcaa agtgggtttt gctgaggata
    32821 acagctggcg ttttaacccc agctacatgc cgccgacgct ggcgcagtat ttcacccgct
    32881 ttggcgcgcc gtggaccacg ctgcgcgaaa ccaatcaaca tttattgctg gaaaccgccc
    32941 cgaaaggctt ttcgccagac tgggtgcgct atgagaaaga caaaggctgg cagctaaaag
    33001 ccgaaaaaac attgatcagc agctacgacg ctatccgcgt ttacatgtgg gtaggcatga
    33061 tgcctgacag cgatccgcaa aaagcgcgga tgctcaaccg gtttaaaccg atggcgacat
    33121 tcactgagaa aaacggttat ccgccggaaa aagtggatgt ggctacgggg aaagcgcagg
    33181 gtaaaggacc ggtcggtttt tctgccgcca tgctgccttt tttacaaaac cgcgatgcgc
    33241 aggcagttca gcgccagcgc gtggccgata actttcccgg cagcgatacc tattacaact
    33301 atgtgctgac cctgtttgga caaggctggg atcaacaccg tttccgcttc tcgacaaaag
    33361 gtgagttatt acctgactgg ggccaggaat gcgcaaattc acactaaaca tattcacgct
    33421 ttccctcggt ctggccgtca tgccgatggt cgaggcagca ccaaccatca gcagaccctg
    33481 cggatggaca gcggcaacac taatgccgtg cgcgggctgg cgaatattta ccgccagcag
    33541 tcgccagaaa aagctgaagc gtttatcgcc tcgctctctg ccagccagcg gcgcagcatt
    33601 gatgatatcg aacgcagcct gcaaaacgac cgtctggcac agcaggcaga ggtactggaa
    33661 aaccagggca aatgggcgca ggcggcagca cttcagcggc aacgactggc gctgggcccc
    33721 ggcagcgtat ggattactta ccgactttcg caggatctct ggcaggccgg acaacgcagc
    33781 caggccgata cgttaatgcg caatctggcg cagcagaagc cgaacaaccc ggagcaggtt
    33841 tacgcttacg ggctgtacct ctcaggtcac aaccaggaca gagcggcgct ggcgcatatc
    33901 aacagcctgc cgcgcgcgca gtggaacagc aatattcagg agctggttaa tcgactgcaa
    33961 agcgatcagg tgctggaaac cgctaatcgc ctgcgagaaa gcggcaaaga ggctgaagcg
    34021 gaagcgatgc tgcgtcagca accaccttcc acgcgtattg acctcacgct ggctgactgg
    34081 gcgtaacaac gacgtgatta caccgccgcc cgcactgcat atcagaatgt cctgacgcgg
    34141 gagccaacta acgccgatgc cattcttggt ctgacggaag tggatattgc tgccggtgac
    34201 aaagcggcgg cacgtagcca gctggcgaaa ctgcctgcca ccgataacac ctcgctgaac
    34261 actcagcggc gcagcagacg tttaataagt tgatcccgca ggcaaaatct cagccaccgt
    34321 cgatggaaag cgcgatggta ctgcgcgacg gtgcgaagtt tgaagcgcag gcgggcgatc
    34381 caaagcaggc gctggaaacc tacaaagacg ccatggtcgc atccggtgtg actacgacgc
    34441 gtccgcagga taacgacacc tttacccgac tgacccgtaa cgacgagaaa gatgactggc
    34501 tgaaacgcgg cgtgcgcagc gatgcggcgg acctctatcg ccagcaggat cttaacgtca
    34561 cccttgagca cgattactgg ggttcgagcg gcaccggtgg ttactccgat ctgaaagcgc
    34621 acactaccat gttgcaggtg gatgcgccgt attctgacgg gcggatgttc tttcgcagtg
    34681 atttcgtcaa tatgaacgtc ggcagtttct ccactaatgc cgatggcaaa tgggatgaca
    34741 actggggcac ctgtacatta caggactgta gcggcaaccg cagccagtcg gattccggtg
    34801 ccagcgtggc ggtcggctgg cgaaatgacg tctggagctg ggatatcggt accacgccga
    34861 tgggcttcaa cgtggtggat gtggtcggcg gcatcagtta cagcgatgat atcgggccgc
    34921 tgggttacac cgttaacgcc caccgtcggc ccatctccag ttctttgctg gcctttggtg
    34981 ggcaaaaaga ctccccgagc aataccggga aaaaatgggg tggcgtacgt gccgacggtg
    35041 tggggctaag tctgagctac gataaaggtg aagtaaacgg cgtctgggca tcgcttagtg
    35101 gcgaccagtt aaccggtaaa aatgtcgaag ataactggcg cgtgcgctgg atgacgggct
    35161 attactataa ggtcattaac cagaacaatc gccgcgtcac aatcggcctg aacaacatga
    35221 tctggcatta cgacaaagat ctgagtggct actcactcgg tcagggcggt tactacagtc
    35281 cgcaggaata cctgtcgttt gccataccgg tgatgtggcg ggagcgcacg gaaaactggt
    35341 cgtgggagct gggggcgtct ggctcgtggt cgcattcacg caccaaaacc atgccgcgtt
    35401 atccgctgat gaatctgatc ccgaccgact ggcaggaaga agctgcgcgg caatccaacg
    35461 atggcggcag cagtcagggc ttcggctaca cggcgcgggc attacttgaa cgacgtgtta
    35521 cttccaactg gtttgttggt acggcaattg atatccagca ggcgaaagat tacgcaccca
    35581 gccatttcct gctctacgta cgttattccg ccgccggatg gcagggtgac atggatttac
    35641 cgccgcagcc gctgatacct tacgccgact ggtaagtttt cagatagcgc ctctcttaat
    35701 gccgctgcga tcgggtatac tcgggcggca atctgggatt tccgggggga gacaatttgc
    35761 gcgtaagtcg ctcgttaaca atcaagcaga tggcaatggt ggcagccgtt gtcctggtgt
    35821 tcgtttttat tttttgcacc gttttgctgt tccatctggt ccagcagaat cgctataaca
    35881 cggctacgca actggaaagc attgctcgct ctgtccgcga acccttatct tcagctattt
    35941 tgaaaggcga tattcccgaa gcggaagcta ttcttgccag cattaaaccg gcaggcgtgg
    36001 tcagccgtgc cgatgtagtg ctgcctaacc agttccaggc gctgcgtaaa agttttattc
    36061 cagagcgtcc ggtgccggta atggttactc gcctgtttga gctaccggtt caaatctcgc
    36121 tgggcgtcta ctcgctcgaa cgtccggcaa atccgcagcc aatagcctat ctggtgctac
    36181 aggcggattc cttccgtatg tataagttcg tgatgagcac tctctcaacg ttagtgacca
    36241 tttacttact tttgtcgctt atcctgaccg tcgccatcag ctggtgcatt aaccgcctga
    36301 ttttgcatcc gttacgcaat attgctcgcg aacttaacgc catcccagcc caggagcttg
    36361 ttggtcacca actggcatta ccgcgtctgc atcaggacga tgaaatcggt atgttggtgc
    36421 gcagttacaa cctcaaccag caattgctgc agcgccatta tgaagaacag aacgaaaatg
    36481 cgatgcgctt cccggtgtcg gatttgccga acaaagcctt gctgatggag atgctggagc
    36541 aggttgtcgc gcgtaaacaa accaccgcgc tgatgatcat cacctgtgaa accctgcgtg
    36601 atactgcggg cgtgctgaaa gaggcgcaac gagaaattct gctgctgacg ctggtggaaa
    36661 aactcaaatc ggtactgtcg ccacgtatga tcctcgcgca gattagcggt tatgactttg
    36721 ctgtcattgc caacggtgta caggaaccgt ggcacgcaat cactttgggt cagcaagtac
    36781 tcactatcat gagcgagcgc ctgccgattg aacgtattca actccgtccg cactgtagca
    36841 ttggcgtggc gatgttctac ggcgatctca ccgccgaaca gctttacagt cgcgctattt
    36901 ctgcggcatt taccgctcgc cataaaggca agaatcagat tcagttcttt gatccgcagc
    36961 agatggaagc agcccagaag cggttgacgg aagagagcga tatccttaat gcactggaaa
    37021 atcatcagtt tgcaatttgg ttacagccac aggtcgagat gaccagcggt aaactggtca
    37081 gtgcggaagt gttactgcgt atccagcaac cggatggcag ttgggacctg ccggatggct
    37141 taatcgatcg cattgagtgc tgtgggctga tggttaccgt cggtcactgg gtgctggaag
    37201 agtcctgtcg attgcttgca gcctggcaag agcgcggcat tatgctgccc ttgtcggtaa
    37261 acttctctgc gctgcaactg atgcacccga atatggtggc ggatatgctg gaactgttaa
    37321 cccgctatcg cattcagccg ggaacactga ttctggaagt gacagaaagc cgacgtattg
    37381 acgaccctca tgctgcggtg gcaatcctcc gtccgctgcg caatgccgga gttcgggtgg
    37441 cgctggatga tttcggcatg ggctacgcag ggctgcgtca gctgcagcat atgaaatcgt
    37501 tgccaatcga cgtactgaaa atcgacaaaa tgtttgttga aggcttgccg gaagatagca
    37561 gcatgattgc tgcaattatc atgctggcgc agagcctgaa cttacaaatg attgccgaag
    37621 gcgtggagac tgaagcacaa cgagactggc tggcaaaagc gggcgttggt attgcccagg
    37681 gcttcctttt tgctcgccca ctccctattg aaatcttcga agagagttac ctggaagaaa
    37741 agtagctacc ccaaactgat tacaaaactt taaaaagtgc tggtttgtgc gagccagctc
    37801 aaacttttta acctttttgt ttcaattatg atccaggtac atttctgtga tgttgtctgg
    37861 gtgttatttt aaggccgcag gtacccccca taaccttaca agacctgtgg ttttactaaa
    37921 ggacacccta tgaaaacctc tctgtttaaa agcctttact ttcaggtcct gacagcgata
    37981 gccattggta ttctccttgg ccatttctat cctgaaatag gcgagcaaat gaaaccgctt
    38041 ggcgacggct tcgttaagct cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc
    38101 gtaacgggca ttgcgggcat ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca
    38161 ctgctttact ttgaaattgt cagtaccatc gcgctgatta ttggtcttat catcgttaac
    38221 gtcgtgcagc ctggtgccgg aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta
    38281 gcggtttacg ccgatcaggc gaaagaccag ggcattgtcg ccttcattat ggatgtcatc
    38341 ccggcgagcg tcattggcgc atttgccagc ggtaacattc tgcaggtgct gctgtttgcc
    38401 gtactgtttg gttttgcgct ccaccgtctg ggcagcaaag gccaactgat ttttaacgtg
    38461 atcgaaagtt tctcgcaggt catcttcggc atcatcaata tgatcatgcg tctggcacct
    38521 attggtgcgt tcggggcaat ggcgtttacc atcggtaaat acggcgtcgg cacactggtg
    38581 caactggggc agctgattat ctgtttctac attacctgta tcctgtttgt ggtgctggta
    38641 ttgggttcaa tcgctaaagc gactggtttc agtatcttca aatttatccg ctacatccgt
    38701 gaagaactgc tgattgtact ggggacttca tcttccgagt cggcgctgcc gcgtatgctc
    38761 gacaagatgg agaaactcgg ctgccgtaaa tcggtggtgg ggctggttat cccgacaggc
    38821 tactcgttta accttgatgg cacatcgata tacctgacaa tggcggcggt gtttatcgcc
    38881 caggccacta acagtcagat ggatatcgtc caccaaatca cgctgttaat cgtgttgctg
    38941 ctttcttcta aaggggcggc aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg
    39001 ctctctgcgg tgggccattt gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc
    39061 tttatgtcag aagctcgtgc gctaactaac ctggtcggta acggcgtagc gaccattgtc
    39121 gttgctaagt gggtgaaaga actggaccac aaaaaactgg acgatgtgct gaataatcgt
    39181 gcgccggatg gcaaaacgca cgaattatcc tcttaatctc accacttatg cccgcactcc
    39241 cttccccgag tgcgggtgtt tgcgcataat tgacccctgt gcctgaactt accacctggg
    39301 tgagttcagg tttatttcac ttcttccgtg acattttcat gttcttgcgg tctaacacga
    39361 agtgttttta acgtcattta gactgaccga caacgggcag tttttttatt accaggatag
    39421 ttgatcaggg gttcacatgc agggcataaa aattcgactt ttagcgggcg gtttgctgat
    39481 gatggccact gctggctatg tgcaggcaga tgcgctccag cctgatccag catggcaaca
    39541 ggggacgctt tccaacggtt tacagtggca agtgctgacc accccccagc gtcccagcga
    39601 tcgtgttgaa attcgcctgc tggttaatac cggttcgctc gccgaaagta cacaacagag
    39661 cggttacagt cacgccatcc ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc
    39721 acaggcgcgt tcattgtggc agcaggggat cgaccctaaa cgcccgatgc cgccggtaat
    39781 tgtctcttat gacaccacgc tgtttaatct gagtttgccc aataaccgta acgatttgct
    39841 gaaagaagcg ctctcttatc tggcaaatgc cactggcaaa ttgaccatca caccagaaac
    39901 catcaaccac gcgctgcaaa gtcaggacat ggtggcaacc tggcctgccg atactaaaga
    39961 gggctggtgg cgctatcgtc tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc
    40021 gctgaaacaa cccgttgaag cggaaaaaat taaagatttc tatcagaaat ggtacacccc
    40081 ggatgcaatg acgctactgg tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat
    40141 caacaaaacg tttggcgaac tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct
    40201 ttctccgctg cgtgcggaag cggtgagtat tatgactgac gcggtgcgtc aggaccggtt
    40261 atctatcatg tgggatacgc cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta
    40321 ctggcgtgcg gacctggccc gcgaggcgct gttctggcat gttcagcaag cgttaagcgc
    40381 cagtgacagc aaagacatcg gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca
    40441 gtgtgccatc aacatcgaat caccaaacga caagctgaac agcaacctta atctggtggc
    40501 gcgtgaactg gcgaaggttc gcgataaagg tctgccggaa gaagagttca atgcgttagt
    40561 ggcgcaaaag aaactggagc tgcagaaact gtttgccgcc tatgcacggg ctgataccga
    40621 tattctgatg ggccagcgga tgcgttcgtt gcaaaatcag gtcgtcgata tcgcgccgga
    40681 gcagtatcag aaactgcggc aggatttcct taacagcctg actgtggaga tgttaaatca
    40741 ggatctgcgt cagcagttgt cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga
    40801 gccggaattt aacatgaaag cgttgcaggc ggcctgggat caaatcatgg ccccatctac
    40861 cgccgctgcg accacctctg tcgccacgga tgacgtacat cctgaagtga cggatattcc
    40921 acctgcacag taatgcctga tggtgatgag ttagcacaac catcaggtat ttatggatga
    40981 gctggataga ggggtaacac tttatccagc cttttgcata tgctgcgttt acgctggcat
    41041 cgcctcacgc gggataatcg cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc
    41101 gcgtttcgcc gcgttttccg cactgccgcc cgtcaaacgt accgccagat aaccggcgct
    41161 gaaagagtcg ccagctgcgg tggtatcgat cactttttct ttcggcagtt tcaccgccgg
    41221 aacatcaact aacgcttcac cagcaatgga caccaggcaa gaatccgccc cgcgtttcac
    41281 caccacttct ttcacgcccg cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg
    41341 accccacagc gcgtcttcgt cgtccagcgt caggaaggcg atatccgtgc attccagcat
    41401 ttgctggtac acctgctgtg tctcttcttt gctggcccac aggcgcggac gatagttatt
    41461 gtcgaaaatc acttttccgc ctttggcgcg gcattcgcgc agcagggaaa gcagcttttc
    41521 gcggctggtc gggcttaaga tcgccaggct aatcccgctc aggtagagat aatcgaaatt
    41581 cgccagctct tcgcaaatcg ccgcagactg ctcgctcgcc agccagaatt tggcggcggc
    41641 ttcgttacgc cagtagtaga acgtacgttc gccggtgctg tcggtttcaa tgtagtaaag
    41701 gcccggaaga cggttttcca tccgttgggt cagggaagta tcaacgttct cgccgtgcca
    41761 ggcgtccagc atctgctggc taaaactgtc cgttcccagc gccgttacgt aatgaacggt
    41821 taatgccgca ggatcgacct gacgggcgat atagacggaa gtgttcaggg tatcgccgcc
    41881 gaaaccgcgc ttaacgtccg cgcctttctc ggaaagctca atcatgcatt cgccaatcac
    41941 ggcaatcttt ttggacatag tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc
    42001 tgtggtggtt tgctggtcaa ccatattaaa acagtgttcc attatttttt tgagccagaa
    42061 cgtattcctg aaagattccg ttgtggagtg aggaaattta ccccggccag gcggtaaagt
    42121 tctgccctta cgcgccgata atctttgtcg agtccgggca gcatcacttt taaacacagg
    42181 acatctttga tgataaggca ggttatccag cgaataagca accctgaagc aagcatcgag
    42241 agcttgcagg aacggcgttt ttggttgcag tgtgagcgtg cttacacctg gcagccgatc
    42301 tatcaaacat gcgggcggtt aatggccgtg gagctattaa cggtggtcac gcatcccttg
    42361 aacccttcgc aacgcctgcc gccggatcgc tattttactg aaatcaccgt cagccatcgg
    42421 atggaggttg tgaaagagca gattgatttg ctggcgcaaa aagccgactt ctttgtagag
    42481 cacggcctgc tggcatcggt caatattgat ggccctacgc tcatcgccct gcgtcagcaa
    42541 ccaaaaatcc tgcgccagat tgagcgtctt ccctggctgc gtttcgaact ggtggagcat
    42601 atccgtctgc cgaaagattc aacctttgcc tcgatgtgtg aatttggccc gctgtggctg
    42661 gatgattttg gtaccgggat ggcaaatttc tctgcactaa gtgaagtgcg ttatgactac
    42721 atcaaaatcg cgcgagaact gtttgtgatg ctgcgtcagt cgccggaagg ccgcacactc
    42781 ttttctcagc ttttacatct aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta
    42841 gaaacaccgg aagagtggcg tgatgttcag aactcgcccg cattcgccgc acaaggctgg
    42901 tttctttcac gcccggcacc gatggaaacg ctgaatacgg cggttctggc gctataagct
    42961 gcctcatttt ccgccgggct ggactatctt taggactggc acaggaagaa tatgaggaag
    43021 cgaacgatga gcaaggcagg caaaataacc gctgcgattt caggggcttt cttgttgttg
    43081 attgtcgtgg cgatcatttt gattgcaact tttgactgga atcgactcaa accgaccatc
    43141 aaccagaaag tctctgcgga gttgaatcgt ccgttcgcta tccgtggcaa tctgggcgtg
    43201 gtgtgggagc ggcaaaaaca agaaactggc tggcgcagct gggtgccgtg gcctcatgta
    43261 catgcagaag acatcattct tggtaatcca ccggatattc ccgaagtcac gatggtgcat
    43321 ttgccgcgag tagaggcaac gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg
    43381 ccgtggatca agctcgaaaa gcccgacgcg cgcctgattc gcctctctga aaagaacaat
    43441 aactggacgt ttaatcttgc caacgatgac aacaaagacg cgaatgcaaa gccgtcggca
    43501 tggtcgtttc ggctggataa tattcttttc gatcaagggc ggatcgccat tgatgacaaa
    43561 gtaagcaaag cggatctgga aatttttgtt gatccgttag gcaagccgct gccattcagc
    43621 gaagttactg gatcgaaagg taaagcggat aaagaaaagg tgggcgatta catttttggc
    43681 ctgaaggcgc agggacgtta taacggtgaa ccgctcacgg gtacgggaaa aataggcggt
    43741 atgctggcgc tgcgtggcga agggacgccg tttccggtac aggctgattt ccgttcaggt
    43801 aatacccgtg ttgcttttga tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat
    43861 ttacggctta aattttctgg cgattcactg ggtgatctct atgaactgac gggcgttctg
    43921 ctgcccgata ccccgccgtt tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa
    43981 aaatcgtcgg tctttgatta tcgcggtttt aatgggcgaa ttggcgatag cgatatccac
    44041 ggttctctgg tctacaccac cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg
    44101 cggcaattgc ggctggcgga cctgggaccg ttgattggcg ttgattccgg gaaaggggca
    44161 gaaaagtcga aacggtctga acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa
    44221 gtgctgcctt atgaccgctt cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc
    44281 ttcaaagggc ggcgcattga gcatggcagt agcctgccga ttagcgatct ttctactcat
    44341 atcatcctca aaaatgctga cctgcgcctg caaccgctga aatttggcat ggcgggcggc
    44401 agcattgcgg cgaatattca tctggaaggc gataaaaagc cgatgcaggg gcgggcagat
    44461 attcaggctc gtcgactgaa actgaaagaa ctgatgcccg atgtggaact gatgcagaag
    44521 acgctggggg aaatgaacgg tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg
    44581 cttttaggca acagtaacgg caacctgaaa ctgttgatga atgacgggct ggtgagccgc
    44641 aacctgatgg agattgttgg gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt
    44701 gacgatgagg tgcgggtgaa ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg
    44761 cgcccgcaga tttttgcttt cgatactgag aacgcgttga ttaatgttac cggcacggca
    44821 agttttgctt cggaacagct ggatttgact attgatccgg agagtaaagg gattcggatt
    44881 atcacactgc gttcgccgct gtatgtgcgt gggacgttta aaaatccgca ggctggggtg
    44941 aaagccggac cgctgattgc ccgtggtgct gttgctgcgg cactggcaac gctggtaaca
    45001 ccggcggcgg cgttactggc actgatctca ccttccgaag gggaggctaa tcagtgtcgg
    45061 acgatattgt cgcagatgaa gaagtgatga taattgccgg atgtctgaca tccggcaatt
    45121 aaacagattt agatttttgg caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc
    45181 ttatccggcc tacaaatcct tgtgaattca atatattgta tggaaattca gtcctgataa
    45241 gcgtagcgca tcaggctttt cacacttatt acaacgactg atgtcgcgtc tcatgggtca
    45301 gcagcagggc gattaacgtc aagccagcca tcgccgccag atataacccc accgcaccta
    45361 acccgtagtt agcctgcaac caggctgcga tatacggcgc aacggaagcc ccgagaatcg
    45421 acgatacgtt gtaagagaac gatgctccgg tgtaacgcac ttctgtcgga aacagctctg
    45481 gtaacagcgc acccatcggc ccgaaggtca gccccatcag acttaacccc agcagcagga
    45541 aggcaaaaac cagaatcggg ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga
    45601 acaggatgat cagcgtggtg atgatcacca tgcttttacg gcgaccaaag gcatcagcca
    45661 gtaatccggc gactggcacc atcacgccaa aaccaataac tgccatcatc agcatccaca
    45721 acacttcgtt acgcggcagg ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag
    45781 agtagaccgt catgatgtaa aacagcgtat aggttgccag cataatgaac gtacccagta
    45841 cggttacgcg aacatgtttg gtcagcagcg tacccagcgg gatcttcacc tgttttttcg
    45901 ctttagcgac tttctcaaac accggcgact catgcagcga cacgcgaaca tacaggccga
    45961 taatgaccag caccgccgag aagataaatg gcacacgcca gccccagctc ataaactgct
    46021 catcggtcag tagccaggaa agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg
    46081 gtgcgcctag ctgtgggaag gagccataca gtgcacgttt gcgcggtggg gcgttttcag
    46141 tcgccagcag cgccgcaccg ccccattcac cgcctaagcc cagaccctga ccaaatcgag
    46201 ccagcgccag cagcagtggg gcgaaaatac caatcgtggc atagcccggc agcagaccaa
    46261 tcaccacggt cgaaatcccc atcgttagca acgaggcgac cagcgtcgct ttacgcccaa
    46321 cgcgatcgcc aaaatgacca aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg
    46381 cgaaggtggc gagcgactgt agcgttgctg ccgttggatc gccctgcggg aagaagatat
    46441 gcggaaacac aataacggcc gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg
    46501 tgccaatgag agaggcgaca aggactttat tacgcgagtt gatcggcgtg tattcttgtt
    46561 cgtggtcgag tgttgtggct gttgcttgca taatcttttc ttattttttg gctaacgaat
    46621 agccatatta cgcacagcaa aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca
    46681 aaaaccaggc aaaaagggga taatgtttag gccagtgatt tttcttctat gaaatgcttc
    46741 acataatttt ataaatagtg gataaaactg gtttcaggtt aaataaaagt taccaactgg
    46801 atttatatgc tggggttatg agttggagtg aaattgtttg ctcccaaata gatggctgaa
    46861 gaggctctgg ggaagagttt caagaggtta tgtgcgcatt atagccggat gcgatgctgg
    46921 cgcatcttat ccggcctacg gggagccagg ccgaataaaa tcgtatgccg gataaggcgt
    46981 ttacgccgca tccggcctat tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt
    47041 atattcggcg gtggcaatcc acgccgcgca aaacagcgtc aaacgggcga agaagtagaa
    47101 aaatgccatc agccccagca cggaaccaaa tgctgcgcca gagggggatt tcatcagcga
    47161 tggcagggtg taggtcatca cgattttaat cacttcaaaa ccaatagcag ccagaaatgt
    47221 tccgcgaatc agggcttttt tgcgcggacg gtggcgcggc aaccgccaga agatccagaa
    47281 gaaaagcaga tagttggcga agatggaaat cgccaggcca atcaatcgcc acgtcggttt
    47341 cagccactca atgctgttca ggtgcagggc gctaataatc atttgctgcg ccgaaccggc
    47401 aaccgaggtg atcgaaagcg tcacaatcag cgcaatcaac aaaccaatca gtgaaataaa
    47461 atcacgcaga tattttaccc agaacttctc ctgatcttgc ggcgagcgtt cccagacatc
    47521 gcgcgactgg gcacgaatcg cttcacgcag gttacccatc cagttgatgc cggaataaag
    47581 tgccaccgcc aggccgacaa gccctacagt cgtacgctgc tgaacggcgg tgttgatggt
    47641 gtttttcaac gtggcggcta gtgtcggatc gctgatgttt tgcagaattt tgtcgaagat
    47701 atcctgtagc aacatcggat gggaggccag cacaaagccc cctgcggcaa acgacaccat
    47761 caaaatcggg atcatcgaca aaaatgagaa ataggtgata gccgcaccaa actggttacc
    47821 cagccgatcg ttaaagcgtt ctgtcgcgcg aataaggtgc gcaataaccg gttggcgttg
    47881 gactttttcg gcagtggtgg tgacggtttc cagcgcctgg ctaactttgc cgcctttctc
    47941 gctattatcc aactgcttaa tcggctcgtg ctccagatcc tgggtgggac gtttgatctc
    48001 gttttcctgc gtcatccgct cttttgtcct tctgtcgtgg tcattcttaa aaagtatagt
    48061 cggtcagtcc aggtgctctt tcattacgcc agccagccac tgcataaaca ggtttacacg
    48121 ccgggaaagc tcccgacgct gcgggtaaac cagcgaaagg gagagcggct cggcacggta
    48181 gcctggcaac acttcaataa gccgcccggc acgcagggct tcgcgcacgg caatgcgcgg
    48241 gatctgaata atccccagac cggcaagacc ggcggcgaga tagttttcgc tactgtttac
    48301 cgtcaacatg ccgccagact taaaccactg gacgccatta acgctggcaa cctcaaaacc
    48361 taacggatgt acacccaggt gcggtgtata acgcactatt gcgtgtgaag tcagatcatc
    48421 ggggctttgc ggataaccaa agcgcgtcag atagtgcgga ctggcacagt tgaccatggt
    48481 tagtttgccg aggggacggg cgataacgcc atcttccggt aacgcgccag tgcgtatcac
    48541 gcaatcaaaa ccatcatgaa gaatatctac cggacggtta tggctactca gttccagctc
    48601 aattcccgga tgctgataga gaaattccga cagacgcggc agtaacaggt ttttcgcgat
    48661 tcctgacggg atgtcgatgc gtaatttacc gctgatactg gtggcatcct gttgaaacag
    48721 accgtccagt tcgctgagat tactcaacac atcttttgcc cgttgataat aggtcatgcc
    48781 ttctggcgtg agtttgaccc gtcgcgtagt gcgctggaga agctgggtgc caagttgatg
    48841 ttccagtgcc tgtatctggc gcgaaacact tccctttggc aaagcaaaga aatccgccgc
    48901 gcgggaaaaa ctttccagct ccgcgacttt gatgaacaac tgcattgcgt gaattttatc
    48961 catttctttg accgattgtt gttcacagcg aaacagtaac gcgcatatcg cattgtttat
    49021 tgttttttct aacatctaat gccgggtttc acaatgttgt catgacgaat gtggcgattt
    49081 ctcagcagga aaagggcgga atagctttat gagaaaggtg aatcccctta aacagagcgt
    49141 atttttcagt gattcccttt aaagtgaata gaggacgtat tggctatttg tgcttatttt
    49201 tgtctcattg ccatttgtta ttttggcttg tagtcgcgga atttcatttt taattgcatg
    49261 cgtactaacc accaatataa ggaaatcctc aaaattgttt taaatagtgc cctcgacatg
    49321 taatttcagg cgtttagctg taactattta tatcggtaac tattttctac gcgtcatctc
    49381 tgtagaatgg ccaccgccaa atggcgaatt cttatttttt ctcgcgggta atacccttcc
    49441 actacttaag tctttttaac gtattcaggt ggagattcag ttatgcaaat agtcatgttt
    49501 gacaggcagt caatatttat tcatggaata aaaatcagtt tacagcagcg tattccagga
    49561 gtgagtattc agggggtcag tcaggcagac gagttatggc aaaagctgga aagttaccct
    49621 gaagccttag ttatgctcga tggcgatcag gatggtgagt tttgctactg gttgctgcaa
    49681 aaaaccgtgg tgcagtttcc tgaggttaag gtgttaatca cggcgacgga ttgcaataaa
    49741 cggtggttac aggaagttat ccattttaat gtgctggcca ttgtgcctcg cgattcaacc
    49801 gtcgagacat ttgcgttggc ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga
    49861 gactggcgga cgacgccgga aaaggacatt aaagatctca aatcgttaag cgcccgccag
    49921 cgggagattt taaccatgtt agcggcgggc gaatcaaata aggagatcgg cagagcgctg
    49981 aatatcagta ccggaacggt aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg
    50041 aaaaatcgca cccaggcggc aatgatgtta aatatctcct cctg
//
LOCUS       DAEEGA010000022        49591 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEEGA010000022 DAEEGA010000000
VERSION     DAEEGA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49591
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.022"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(33..1211)
                     /gene="setB"
                     /locus_tag="KLH46_001320"
     CDS             complement(33..1211)
                     /gene="setB"
                     /locus_tag="KLH46_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBE0378067.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFWVMTPTY
                     "
     gene            1579..2709
                     /gene="fruB"
                     /locus_tag="KLH46_001321"
     CDS             1579..2709
                     /gene="fruB"
                     /locus_tag="KLH46_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBE0378068.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2709..3647
                     /gene="fruK"
                     /locus_tag="KLH46_001322"
     CDS             2709..3647
                     /gene="fruK"
                     /locus_tag="KLH46_001322"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBE0378069.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3664..5355
                     /gene="fruA"
                     /locus_tag="KLH46_001323"
     CDS             3664..5355
                     /gene="fruA"
                     /locus_tag="KLH46_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBE0378070.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5779..6720
                     /locus_tag="KLH46_001324"
     CDS             5779..6720
                     /locus_tag="KLH46_001324"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBE0378071.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENTECLN"
     gene            6708..7646
                     /gene="psuG"
                     /locus_tag="KLH46_001325"
     CDS             6708..7646
                     /gene="psuG"
                     /locus_tag="KLH46_001325"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBE0378072.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7740..8990
                     /locus_tag="KLH46_001326"
     CDS             7740..8990
                     /locus_tag="KLH46_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBE0378073.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9061..9755)
                     /gene="yeiL"
                     /locus_tag="KLH46_001327"
                     /pseudo
     CDS             complement(9061..9755)
                     /gene="yeiL"
                     /locus_tag="KLH46_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9886..10827
                     /gene="rihB"
                     /locus_tag="KLH46_001328"
                     /pseudo
     CDS             9886..10827
                     /gene="rihB"
                     /locus_tag="KLH46_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
     gene            10927..12177
                     /locus_tag="KLH46_001329"
     CDS             10927..12177
                     /locus_tag="KLH46_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBE0378074.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12233..13321)
                     /locus_tag="KLH46_001330"
     CDS             complement(12233..13321)
                     /locus_tag="KLH46_001330"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBE0378075.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13324..14181)
                     /gene="nfo"
                     /locus_tag="KLH46_001331"
     CDS             complement(13324..14181)
                     /gene="nfo"
                     /locus_tag="KLH46_001331"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBE0378076.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLHTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14255..15304)
                     /locus_tag="KLH46_001332"
     CDS             complement(14255..15304)
                     /locus_tag="KLH46_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBE0378077.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15403..16284
                     /locus_tag="KLH46_001333"
     CDS             15403..16284
                     /locus_tag="KLH46_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0378078.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DEQVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16490..17959
                     /gene="lysP"
                     /locus_tag="KLH46_001334"
     CDS             16490..17959
                     /gene="lysP"
                     /locus_tag="KLH46_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBE0378079.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18251..20242
                     /gene="cirA"
                     /locus_tag="KLH46_001335"
     CDS             18251..20242
                     /gene="cirA"
                     /locus_tag="KLH46_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBE0378080.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20274..21109)
                     /gene="yeiG"
                     /locus_tag="KLH46_001336"
                     /pseudo
     CDS             complement(20274..21109)
                     /gene="yeiG"
                     /locus_tag="KLH46_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21367..22035
                     /gene="folE"
                     /locus_tag="KLH46_001337"
     CDS             21367..22035
                     /gene="folE"
                     /locus_tag="KLH46_001337"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBE0378081.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            22052..23128
                     /locus_tag="KLH46_001338"
     CDS             22052..23128
                     /locus_tag="KLH46_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBE0378082.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23270..24310
                     /gene="galS"
                     /locus_tag="KLH46_001339"
     CDS             23270..24310
                     /gene="galS"
                     /locus_tag="KLH46_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HBE0378083.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24590..25588
                     /gene="mglB"
                     /locus_tag="KLH46_001340"
     CDS             24590..25588
                     /gene="mglB"
                     /locus_tag="KLH46_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBE0378084.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25649..27169
                     /gene="mglA"
                     /locus_tag="KLH46_001341"
     CDS             25649..27169
                     /gene="mglA"
                     /locus_tag="KLH46_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBE0378085.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27185..28195
                     /gene="mglC"
                     /locus_tag="KLH46_001342"
     CDS             27185..28195
                     /gene="mglC"
                     /locus_tag="KLH46_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBE0378086.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28231..29444)
                     /gene="preA"
                     /locus_tag="KLH46_001343"
                     /pseudo
     CDS             complement(28231..29444)
                     /gene="preA"
                     /locus_tag="KLH46_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29438..30676)
                     /gene="preT"
                     /locus_tag="KLH46_001344"
     CDS             complement(29438..30676)
                     /gene="preT"
                     /locus_tag="KLH46_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HBE0378087.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30870..31109)
                     /locus_tag="KLH46_001345"
     CDS             complement(30870..31109)
                     /locus_tag="KLH46_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBE0378088.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31112..31831)
                     /gene="sanA"
                     /locus_tag="KLH46_001346"
     CDS             complement(31112..31831)
                     /gene="sanA"
                     /locus_tag="KLH46_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBE0378089.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31981..32865)
                     /gene="cdd"
                     /locus_tag="KLH46_001347"
     CDS             complement(31981..32865)
                     /gene="cdd"
                     /locus_tag="KLH46_001347"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBE0378090.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32995..33690)
                     /locus_tag="KLH46_001348"
     CDS             complement(32995..33690)
                     /locus_tag="KLH46_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBE0378091.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33687..34085)
                     /locus_tag="KLH46_001349"
     CDS             complement(33687..34085)
                     /locus_tag="KLH46_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBE0378092.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34324..35271
                     /gene="dusC"
                     /locus_tag="KLH46_001350"
     CDS             34324..35271
                     /gene="dusC"
                     /locus_tag="KLH46_001350"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBE0378093.1"
                     /translation="MRVLLAPMEGVLDSQVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISDC
                     DAVMIGRGAFNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35642..35725)
                     /gene="yohP"
                     /locus_tag="KLH46_001351"
     CDS             complement(35642..35725)
                     /gene="yohP"
                     /locus_tag="KLH46_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBE0378094.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35949..37385
                     /gene="mdtQ"
                     /locus_tag="KLH46_001352"
     CDS             35949..37385
                     /gene="mdtQ"
                     /locus_tag="KLH46_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBE0378095.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            37438..38199
                     /locus_tag="KLH46_001353"
                     /pseudo
     CDS             37438..38199
                     /locus_tag="KLH46_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38316..38894)
                     /locus_tag="KLH46_001354"
     CDS             complement(38316..38894)
                     /locus_tag="KLH46_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0378096.1"
                     /translation="MALNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39064..39651
                     /locus_tag="KLH46_001355"
     CDS             39064..39651
                     /locus_tag="KLH46_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBE0378097.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39825..40757
                     /gene="pbpG"
                     /locus_tag="KLH46_001356"
     CDS             39825..40757
                     /gene="pbpG"
                     /locus_tag="KLH46_001356"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBE0378098.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40795..42510)
                     /gene="dld"
                     /locus_tag="KLH46_001357"
     CDS             complement(40795..42510)
                     /gene="dld"
                     /locus_tag="KLH46_001357"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBE0378099.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42706..45003
                     /gene="bglX"
                     /locus_tag="KLH46_001358"
     CDS             42706..45003
                     /gene="bglX"
                     /locus_tag="KLH46_001358"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBE0378100.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45255..46172
                     /gene="osmF"
                     /locus_tag="KLH46_001359"
     CDS             45255..46172
                     /gene="osmF"
                     /locus_tag="KLH46_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBE0378101.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     FQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46179..47336
                     /gene="yehY"
                     /locus_tag="KLH46_001360"
     CDS             46179..47336
                     /gene="yehY"
                     /locus_tag="KLH46_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBE0378102.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTVRQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47329..48255
                     /gene="yehX"
                     /locus_tag="KLH46_001361"
     CDS             47329..48255
                     /gene="yehX"
                     /locus_tag="KLH46_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBE0378103.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48260..48991
                     /gene="yehW"
                     /locus_tag="KLH46_001362"
     CDS             48260..48991
                     /gene="yehW"
                     /locus_tag="KLH46_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBE0378104.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48972..49079)
                     /locus_tag="KLH46_001363"
     CDS             complement(48972..49079)
                     /locus_tag="KLH46_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBE0378105.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49139..49591)
                     /locus_tag="KLH46_001364"
                     /pseudo
     CDS             complement(49139..49591)
                     /locus_tag="KLH46_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11977 a        12212 c        13319 g        12083 t
ORIGIN      
        1 gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat tacccaaaac
       61 agagtggcga taatcatcac catcgcaaac cagaacacag cgtgataatt ccagatctcg
      121 gcgacgatgc ccgccactga tcctgcgatg atccagccca cgcgcgacgt gttggtatag
      181 agcgtggtgg ctgaacctgc ctgaccgggc atcaaatcct gaaaatagag catcccgatg
      241 ccgcccagaa tgccaataaa aatagcattt agcagctgca agcccaacag aatggcaggt
      301 gaatgcgcca tcagcattcc tgcgtaaaaa cagacgccac ccacggcagc aacgcgcatt
      361 aagaaacgct tacccagacg tttggcgaaa tatccggcaa tcaacatcgt cgggatttcc
      421 agcccggcgg cggtccccat catcacaccg gctagtttct cgggaagatg cagttcgttg
      481 ataataaata gcggcatgtt gatgatgtac aggctgttcg agccccacat caatgtacaa
      541 atgacaaaca gcagtagcgt atcgcgacgg ttacggcgcg gcgcttcaac cgtgccggtc
      601 gccagcggaa gctctttttg catcgacggt aaaaacagcc acaccatcac gccgcaaaca
      661 ataaacgcta ccgctgcgct cagatacatt accgtaaagc tgaaacccat cgctaacgca
      721 taagccagcg gtgggccaat gacccatgcc agtgaaacct gagcgcgtaa aaaagagctg
      781 aacatcaccg cctcacgtcc ggttttgtcg gcatgttcac gggcaagggc aaacatttgc
      841 gggttagcgg tcgagccaaa gctgctaaga aagacgccaa cgaatagcaa aacaaagtag
      901 ttgcgattcc aagcaaaaag ggtgcaggcc agcacgccta acaggcagca aaagacaatc
      961 agcgatttgc gatcgccgcg cttatcagag cgcccagcga gaaactgact taccagaatc
     1021 ccaatgacag cgctgccggt aaagaagaat cccaccatcg ccggacgggc atgtacttca
     1081 tcggtaagga aaatactgag tgtcggggtt tgcagagcac ccgcaatacc ggtgagaaag
     1141 gcaacgatta aaaacgccgt cgaggtcagg tcaaacgatt tcgcgctgga gactgcgggg
     1201 gagttatgca tgctggtcga tcactggaag aggaagacgc ggagtttacg ctgcttgtca
     1261 gaaaataaac agacagcgaa atcaaaagcg ccacgaataa tcaaaatgcc atttcactgt
     1321 tgctgattgg ctgctgacgc tgctgaaata aaattgctga taacggattt tcccatcagc
     1381 aattaggaaa aatgacaaaa aattgtgcag cacatcaaac ttttgctcat aactttacgg
     1441 ctttccttgc gtgctgaaac agaagtatta tgctttcttg aaacgtttca gcgcgatctt
     1501 gtctttaacc ctaagctagg ttggcgcttt ttttctcata gaggctgaat cgtttcaatt
     1561 cagcaagaga ggagaactat gttccagtta tccgtacagg acatccatcc gggcgaaaag
     1621 gccggagaca aagaagaggc gattcgccag gtcgctgcgg cgctggtgca ggccggtaat
     1681 gtagcagaag gctacgtcaa tggcatgctg gcgcgcgaac agcaaacctc aacgttcctc
     1741 ggcaatggta ttgctattcc acacggcact accgacaccc gcgatcaggt gctgaaaacc
     1801 ggcgttcagg tatttcagtt cccggaaggc gtcacctggg gtgacggtca ggtagcgtac
     1861 gtggcaatcg gtattgctgc cagctcggat aaacatctgg gcctgctacg ccagctgacc
     1921 cacgtactga gcgatgattc cgttgctgaa caactgaagt cagcaacaac agcagaagaa
     1981 cttcgcgcat tactgatggg cgaaaagcag agtgagcagc tgaagctcga caacgaaatg
     2041 ctgacgctgg atatcgtcgc cagcgatctg ctgactcttc aggcgctgaa cgctgcgcgt
     2101 ctgaaagagg cgagggcagt tgacgccact ttcgtcacca aagccatcaa tgaacaaccg
     2161 ctgaatctcg gacagggtat ctggctgagc gatagcgccg aaggcaatct gcgtagtgcg
     2221 attgcggtaa gccgtgcggc aaatgctttt gatgtggacg gcgaaacggc agccatgctg
     2281 gtgagtgtgg cgatgaatga cgatcagccc atcgcggttc ttaagcgtct cgctgatttg
     2341 ttgctcgaca ataaagctga ccgcttgctg aaagcggatg cggcaacgtt gctggcgctg
     2401 ctgaccagcg atgatgcgcc gaccgacgac gtgttaagcg cggagtttgt agtgcgcaat
     2461 gaacacggcc tgcatgctcg tccaggtacc atgctggtca ataccattaa acaatttaac
     2521 agtgatatta ccgtgacaaa ccttgatggc accggcaaac cggcaaacgg acgtagtctg
     2581 atgaaagttg tggcacttgg cgttaagaaa ggtcatcgcc tacgctttac cgcccagggt
     2641 gcagatgctg aacaggcgct gaaagccatt ggcgacgcta tcgctgctgg tcttggggag
     2701 ggcgcataat gagcagacgt gttgctacga tcacccttaa tccggcttat gaccttgttg
     2761 gtttctgccc ggaaattgaa cgcggcgaag tgaacctggt gaaaaccacc ggtctgcatg
     2821 cggcgggtaa aggcatcaac gtggccaaag tattaaaaga cctgggaatt gatgtcaccg
     2881 ttggcggctt cctgggtaaa gacaatcagg atggttttca gcaactgttc agcgagctgg
     2941 gcattgccaa ccgtttccag gttgtacagg ggcgcacccg aattaacgtt aagctgacgg
     3001 aaaaagacgg cgaagtgacc gacttcaact tctcgggttt cgaagtcacc cccgccgact
     3061 gggaacgctt tgtgactgat tctctgagct ggctcggtca gttcgatatg gtctgtgtca
     3121 gcggaagctt accgtcaggc gtcagcccgg aagcgttcac cgactggatg actcgcctgc
     3181 gtagtcagtg tccttgcatt atctttgata gtagccgtga agcgttagta gcaggtttga
     3241 aagcggcacc gtggctggtg aaacctaacc gccgcgagtt ggaaatctgg gcaggccgta
     3301 aactgcctga aatgaaagat gtgattgaag ctgcacatgc gctacgtgaa caaggtattg
     3361 cgcatgttgt tatttcactg ggtgccgaag gcgcgctttg ggttaatgcc tccggcgaat
     3421 ggatcgctaa accaccgtca gtcgatgtcg taagcaccgt tggcgcaggg gattctatgg
     3481 ttggtggcct gatttatggc ttgctgatgc gtgaatccag tgaacacaca ctgcgtctgg
     3541 cgacagctgt tgcagccctg gcggtaagtc aaagcaatgt gggtattacc gatcgtccgc
     3601 agttggccgc aatgatggcg cgcgtcgact tacaaccttt taactgacag caggagaggc
     3661 ataatgaaaa cgctgctgat tattgacgct aatctcggtc aggcacgcgc ctatatggcg
     3721 aagaccctgc tgggcgcggc ggcgcgaaaa gcaaaactgg aaatcatcga caatccgaac
     3781 gacgcagaaa tggcgattgt tctcggtgat tccatcccga atgacagcgc gctgaacggt
     3841 aaaaatgtct ggctgggtga tatttcccgg gcagttgcgc accctgagct gttcctgagt
     3901 gaagccaaag gccatgcgaa accttacact gcgccggtca ctgcgacagc accggttgcc
     3961 gccagcggtc cgaaacgcgt agttgcggtg actgcttgcc cgactggcgt agcacacacc
     4021 tttatggcgg ctgaagccat tgaaaccgaa gcgaaaaaac gtggctggtg ggtgaaagtt
     4081 gaaacccgtg gttctgttgg cgcgggtaat gcaatcactc ccgaggaagt agccgcagcg
     4141 gatctggtga ttgtggcggc agatatcgaa gtggatctgg cgaaatttgc tggtaaaccg
     4201 atgtatcgta cctctaccgg tctggcgctg aagaaaacct cgcaggaact ggataaagcg
     4261 gttgctgaag caacgccgta tgaaccggcg ggcaaagctc aaacggcgac cactgaaggt
     4321 aagaaagaga gtgcaggcgc ttatcgtcac ttgctaacgg gcgtctctta catgctgccg
     4381 atggtcgttg ctggtggtct gtgtatcgcg ctttcttttg cttttggtat cgaagcgttt
     4441 aaagagccgg gtacgttggc agcggcgctg atgcagattg gtggtggttc agcctttgcg
     4501 ctgatggtgc cggtactggc aggttatatt gccttctcca ttgccgatcg tccgggcctc
     4561 acgccgggtc tgattggcgg tatgctggcg gtcagcaccg gttctggctt cattggcggt
     4621 attattgcgg gcttcctggc tggttacatt gcgaagttaa tcagtacgca actgaaactg
     4681 ccgcagagta tggaggcgct gaaaccgatc ctgatcattc cgctaatttc cagtctggtg
     4741 gtcggtctgg cgatgatcta cctgatcggt aaaccagttg ctggcattct cgaagggctg
     4801 actcactggc tgcagaccat ggggactgcg aatgcggttc tgctgggggc gatcctcggt
     4861 ggcatgatgt gtactgacat gggcggtccg gtaaacaaag cagcgtacgc attcggtgtg
     4921 ggtctgctga gtactcaaac ctatggcccg atggcggcga ttatggcggc aggtatggtg
     4981 ccaccgctgg caatgggcct ggcaacaatg gtggcgcgtc gcaaattcga caaagcgcag
     5041 caggaaggtg gcaaagccgc tctggtattg ggactgtgct tcatttcgga aggtgcaatt
     5101 ccgtttgctg ctcgtgatcc gatgcgtgtg ctgccgtgct gtatcgtggg tggggcgctg
     5161 actggcgcaa tctcaatggc gattggtgcg aaactgatgg caccgcacgg tggtctgttt
     5221 gttctgctga tccctggcgc tattacgccg gtattgggtt acctggtagc aattattgcc
     5281 ggtacgctgg tggcgggttt ggcctatgcc ttcctgaaac gtccggaagt ggacgcagta
     5341 gcgaaagcag cgtaataaaa ggtgtgttac agggcagaaa tttcctgccc tgtaacattt
     5401 gatgagcgca aagagaatga ccagcttgtg aatgtaattt tatgaattta aatggtttta
     5461 tctttcagta gaaacttcaa acgttcatca aacactaagg ttatttcctc aagggcagat
     5521 tattttctgc ccttttttag tgttcctgtt ctgtgcatcc cgtcacaaat tcctcgcttt
     5581 tgtaattcag gcatttaatc gcattttgtg agcctgctca aatctgccca ttttgcctct
     5641 ctggcggtaa tttactttac gtaaaacatg catactatga gcacatgttt aaatatgaac
     5701 acatgtttaa accgtctttg cagtatctat attcttgtgg attgacaggg ttggtaattt
     5761 tttaccagag gacgtgctat gcgcgaaaag gattatgtcg taattatagg ttcggcgaat
     5821 attgatgtcg ccggatattc acatgaatca ttaaattatg cggattcaaa tccaggtaaa
     5881 ataaaattta cgcctggtgg agtagggcgc aatattgcac aaaacctggc gttgctgggt
     5941 aacaaagcct ggctactgag cgccgtaggc agtgattttt atggtcaatc gctgctaacg
     6001 caaaccaatc aatctggtgt ttatgtcgat aaatgcctga ttgtgccggg agaaaatacg
     6061 tcgagttatt tatcattact cgataatacc ggtgaaatgc tggttgctat aaatgacatg
     6121 aatattagca acgctattac agctgaatat ctcgcacagc accgtgaatt tattcagagg
     6181 gcaaaggtca ttgtcgcgga ctgtaatatc agtgaagagg cactggcatg gattctggat
     6241 aatgccgcca acgtacccgt atttgtcgat ccggtttcag cgcggaaatg tgtcaaagtg
     6301 cgcgaccgtc taaatcagat ccacactctg aagccaaacc gccttgaagc ggaaaccctg
     6361 agtgggattg cgctgtcagg gcgtgacgat gtggcaaaag ttgctgcctg gttccatcaa
     6421 catggcctga accgactggt attgagcatg ggcggcgacg gcgtttatta cagcgatatc
     6481 agcggtgaaa atggctggtc tgcgccgatc aaaaccaatg ttattaatgt taccggagcg
     6541 ggcgatgcca tgatggcggg acttgcttcg tgttgggtag acggaatgcc gtttgccgaa
     6601 tctgttcgtt tcgcacaggg atgttcgtca atggcgctct cctgtgaata caccaataac
     6661 cccgatttat cgattgccaa cgttatatcg ttagtggaga acacagaatg tctgaattaa
     6721 aaatctcccc tgaattatta caaatttctc cggaagtgca ggacgcttta aaaaacaaaa
     6781 aaccggttgt ggcgctggaa tcgaccatta tttctcacgg gatgccgttc ccacaaaatg
     6841 cccagaccgc aattgaagta gaagaaacta ttcgtaaaca gggcgcagta cctgccacta
     6901 tcgccattat tggcggcgtg atgaaagtgg gtttaagcaa agaaaaaatt gaattactgg
     6961 gtcgtgaagg gcataacgtg accaaagtta gtcgtcgcga tttacctttt gttgttgccg
     7021 ccggaaaaaa tggcgcaacc actgtggctt caacgatgat tattgcggcg cttgccggaa
     7081 ttaaagtatt tgccaccggg ggaattggtg gtgtgcatcg cggggcggaa cataccttcg
     7141 atatttctgc cgatttgcaa gaactggcaa atactaatgt caccgttgtt tgtgccgggg
     7201 cgaaatctat tctcgattta ggattaacca ctgagtattt agaaaccttc ggtgtgccgt
     7261 taattggcta tcagactaaa gcgctgcctg cgtttttctg ccgtaccagc tcgtttgacg
     7321 tcagcattcg tctcgacagc gccagtgaaa ttgcccgtgc aatggcggtg aaatggcaaa
     7381 gcgggctgaa cggtggcctc gtggtagcga acccgatccc ggaacagttt gcgatgccgg
     7441 aggaatctat caatgcagcc atagatcaag ccgtcgccga agccgaagag cagggcgtta
     7501 ttggtaaaga aagtacaccg ttcctgctgg cgcgcgttgc tgagctgacc ggcggtgaca
     7561 gcctgaaatc caatatccag ctggtgttca acaacgccat tctggcgagt gaaattgcca
     7621 aagaatacca gcgtctcgcg ggttaatttc tgacgggcag aaagccgtcg cccgtactca
     7681 tcaagcatac caaaacctgg cgctgacgcc gggtttactg gcatgaaggg aaaatcatta
     7741 tggatataat gagaagtgtt gtggggatgg tggtgttact ggcaatagca tttctgttgt
     7801 cagtgaataa aaagagcatc agtttgcgca cggttggagc cgcactgctg ctgcaaatcg
     7861 ctattggtgg catcatgctc tacttcccac cgggaaaatg ggcagtagaa caggcggcat
     7921 taggcgttca taaagtgatg tcttacagtg atgccggtag cgccttcatt tttggttcgc
     7981 tggttgggcc gaaaatggat gtcctgtttg acggtgcggg ttttatcttc gcctttcgcg
     8041 tacttccggc gattattttc gttactgcgc tcatcagtct gctgtactac attggcgtga
     8101 tggggctgct gattcgcatc cttggcagca ttttccagaa agccctcaac atcagcaaaa
     8161 tcgaatcttt tgttgcggtt accactattt tcctcgggca aaatgagatc ccggcgatcg
     8221 ttaaaccgtt tatcgatcgc gtgaatcgca acgagttgtt taccgcaatt tgtagcggga
     8281 tggcgtccat tgctggttcg gtgatgattg gttatgccgg aatgggcgta ccaattgact
     8341 acctgttagc ggcatcgctg atggcgatcc ccggcggtat tttgtttgca cgtattctta
     8401 gcccggcaac cgagccttcg caggtcacat ttgaaaatct gtcgttcagc gaaacgccgc
     8461 caaaaagctt tatcgaagcg gcggcgagcg gtgcgatgac cgggctgaaa atcgccgctg
     8521 gtgtagcgac ggtggtaatg gcgtttgtcg caattattgc gctgatcaac ggcattatcg
     8581 gcggaattgg cggctggttt ggtttcgcca atgcctctct ggaaagtatt tttggctatg
     8641 tgctggcacc gctggcgtgg atcatgggtg tggactggag tgatgccaac cttgctggta
     8701 gcctgattgg gcagaaactg gcgattaacg aattcgtcgc ttacctgagt ttctccccat
     8761 acctgcaaac gggcggcacg ctggaagtga aaaccgtggc gattatatcc tttgcgcttt
     8821 gtggttttgc taactttggt tctatcggtg ttgtcgttgg cgcattttcg gctatttcgc
     8881 caaaacgtgc tccagaaatc gcccagcttg gtttacgggc gctggcagca gcaacgcttt
     8941 ccaacctgat gagtgcgact attgccgggt tctttattgg tctggcgtaa tgctcgattt
     9001 gtagaccgga taaaacgtat taagcgttgc atccggcaat cgcgctattg caaataattt
     9061 ttactgcatc atcccggaga atttattctc cgggtccatc tccagcgcca gtcctgacaa
     9121 ctgctttctg tttttaatta gatatccttt cttttaatta ataaaccgtc gtgaatgaat
     9181 tgcgcgagaa catataaaag atgtcgataa gaaacgccta aatactctgc cgcttgcgta
     9241 tgcttttcgt gataaagatc accttcttgt gagagtaata taaatgctgc caggcgattc
     9301 actaacggaa atgattgatt ctgagttaaa gaaacaatat tacgataatt tttatgactt
     9361 aaggtgacgc aaagttttcg taaaaatagg gtgtcgttta ataacagcgg acggtaatgt
     9421 ttcataggga gcgcaaggca ccaacactct tcaatagcct gcaccgcacg cggttcatgg
     9481 tctttgtcga ttaactcaat ctcgccaata aaacagggag cggcaaagaa atcgatcagc
     9541 gacacgcgac cattggcaag cgtggcgtaa agcctggcgc ggcctcgcgt cagataaaac
     9601 agccatgacg gctgttgccc ttcctgcaca atgtagtcac gcgctaaaaa atgaaacagc
     9661 cgcgtatcgg ctgaaacatc cgttgaaaag caatccttaa acgcagattc actcatcaat
     9721 tgctgcttaa ggtcattatt gtggatttct ttcacgatgt ttatccgcta tgagatttct
     9781 catatttagc atacgtacgc tgttgttaag attcaatcat tggcccggtc gaattttctt
     9841 tgctgtagaa aaaaataacg caactggaaa cagaggaaat aaacaatgga aaagagaaaa
     9901 attattctgg attgtgagcc gggtcatgat gatgctattg caatgatgat ggcagcgaaa
     9961 catccggcaa tagatttatt aggcatcact attgtagcgg gtaatcagac gcttgataaa
    10021 acattaatta atggcctgaa tgtttgccag aaactggaga ttaatgttcc ggtttatgcg
    10081 gggatgccgc aacccattat gcgtaaacaa atcgttgccg ataatattca cggtgaaacc
    10141 ggactggatg gcccggtatt cgagccgctg acccgctagg cagaaagtac ccatgcggtg
    10201 aaatatatca tcgataccct gatggcaagc gatggcgata tcactctggt gccggttggt
    10261 ccgctttcaa atatcgcggt ggcaatgcgt atgcaacccg caatcctgcc caaaatccgt
    10321 gaaattgtgc taatgggcgg cgcttacggt acaggcaact tcaccccatc tgccgagttc
    10381 aatatctttg ccgacccgga agccgcccgc gtggtgttca cctccggcgt tccattagtg
    10441 atgatgggcc tcgatcttac caaccagacc gtttgcaccc cggatgtaat tgctcggatg
    10501 gaaagggtag gcggccctgc cggagagctg ttcagcgaca tcatgaactt cactctcaaa
    10561 acgcagttcg aatactacgg cctggctggc ggcccggtgc atgacgccac ctgcattggt
    10621 tatctgatca accctgatgg cattaaaacc caggatatgt acgtcgaagt ggacgtcaac
    10681 agcggccctt gctacgggcg tacggtctgc gacgaactgg gcgttcttgg caagcccgct
    10741 aataccaaag tcggcatcac tattgatacc gactggttct ggggcttagt cgaagagtgc
    10801 gtgcgcggct acatcaaaac ccattaagtg ttgataacaa gccgggacag tatcgcaaga
    10861 tgcaggcccg gctatttctg atgggcagaa agccgtcgcc caaaatgaca tgaagagata
    10921 atcactatgg atgtcatgag aagtgttctg ggaatggtgg tactgctggc aattgcgttt
    10981 ttactgtcag taaacaagaa gaagatcagc ctgcgtaccg ttggcgcggc gttagtgtta
    11041 caggtcgtga ttggcgggat tatgctttgg ttaccgccag ggcgttgggt cgcagaaaaa
    11101 gtcgcttttg gcgtccacaa agtgatggcg tacagcgacg caggtagcgc atttatcttc
    11161 ggttctctgg tcggaccgaa aatggatacc ttatttgatg gcgcaggatt tatctttggt
    11221 ttcagggtat taccggcaat tatcttcgtc actgcactgg tgagtattct ctactacatc
    11281 ggtgtgatgg ggatcttaat tcgcattctc ggcggtatct tccagaaagc tttaaatatc
    11341 agcaagatcg agtcattcgt cgcggtcacc accattttcc tcgggcaaaa cgaaattccg
    11401 gcgatcgtca aaccctttat cgatcgtctg aatcgcaatg aattatttac agcgatttgt
    11461 agtggcatgg cctcgattgc tggttcgaca atgattggtt acgccgcact gggcgtacct
    11521 gtggaatatt tgctggcggc atcgttaatg gcgatccctg gcgggatctt gtttgcccgc
    11581 ctgttaagcc cggctacgga atcttcgcag gtttctttta ataacctctc tttcaccgaa
    11641 acaccgccaa aaagcattat tgaagccgat gcgacagggg caatgaccgg gctgaaaatc
    11701 gccgccggtg tagcgacagt tgttatggca tttgtcgcca tcattgcgtt aattaacggt
    11761 attatcggcg gcgttggcgg ctggtttggt tttgaacatg cctcgctgga gtccatttta
    11821 ggttacctgc tggccccact tgcgtgggta atgggtgttg actggagtga tgcaaatctt
    11881 gccgggagtt tgattggaca gaaactggca ataaatgaat ttgtcgctta tctcaatttc
    11941 tcaccctatc tgcaaacggc tggcactctg gatgctaaaa ccgtggcgat tatttccttc
    12001 gcgttgtgcg gtttcgctaa ctttggttct atcggggtgg tggtgggggc gttttctgcg
    12061 gttgcgccac accgtgcgcc ggaaatcgcc cagcttggtt tacgcgcgct ggcggcggcg
    12121 acactttcta atctgatgag tgccaccatt gccgggttct ttattggttt agcgtagtgt
    12181 gcaatttatg agccggatta gatgcggtca agcgtcgaat ccggcaacat tgttacacca
    12241 ttggcactaa tgacagcgcg ttatcggcag acagggtagg gttgtttaag ctgccgctgg
    12301 cgcgtgaaat tgccgcgcag gccatcgcaa aacgggcgct gtcacggaaa ttgtttccct
    12361 ccagaaagct atataccaga cccgccataa aaccatcgtc agcaccaaaa ctgtctaccg
    12421 tcgtatgcgc tggcacagtc agcaaaaatt gttcaccatc cttttcgctg caataaactg
    12481 actcatcggg caaataaaca aacagttgct gaacaccttg ctgatgcaat gcattcactg
    12541 cggcattacg gtcagcatcg ctggtgatcg cctgtcccca taaaatttcc agctccggta
    12601 aagtgggttt cagggtgtga atatgcgcca gccagtgttt gattttgccc gctttgaatt
    12661 ctgaaacggt atcgacaaac accgggatct catcagcgag ggtaaagacc cattccagcg
    12721 cctcggctgt caggttgcaa tcagccagta ccacgcccgc atgacgaagt aaatcgcgcg
    12781 acccgttcag tagttgcggt gtcaactgtt ccagcagatg ggtatcgtta attgccagca
    12841 cggtttgatc gtctcgattg gcaattgcca gatacgtcga tgtgctttga ccatgcaaac
    12901 gaacgcagcc ggagacattc acgccggcac ggcgcgtttc ttccaggagc atttcgccat
    12961 aaaagtcatc gccaatcact gaaagcaaat ggacgtcacg gcctaacagc gccagattgt
    13021 gggcgatatt gcgtccaacg ccgcctgccg aacaatgaat tgtaccggga tgagaagccg
    13081 cttgcgggta atggatatcc gccatcccgc gaatatccat attgattgtc cccaccacta
    13141 cgcagtattc ctgctcggtg agaatgtaac ctttgtcttt aatccggcct ttgcgcatta
    13201 aatccataat atgcgccgca acgcgcgaac gactgatttg cagcatgtcc gcaatttcgt
    13261 tctgctgaat cagcgggtta cgccgtaaaa ttgcaaggat ctccttttcc cggttattca
    13321 tcttcaggct accgcttttt cagtttgttg cgctttcagc caggcgatct cttctgccca
    13381 gatatccggg ttgatggttt caaggatcag cggaatgccg tcgaaacggt cgttctgcat
    13441 aatccagcgg aacgcatcat gaccgatatt gccttcaccg aggctatgat ggcggtcaac
    13501 gcggctgcca aaggtacttt tcgcatcgtt aaggtgcatc ccgcgcagat acttaaagcc
    13561 gacaatacgg gcaaaatcag cgaacgtttt ttcgcattcg gctggagtat gcaaatcata
    13621 cccggcagcg aaagcatggc aggtatcaat gcagacgccg acgcgggatt tatcttccac
    13681 gccgtcgata atcgccgcga gatgttcgaa tttaaaccct aagttactgc cctgaccggc
    13741 ggtgttttca atcaccgctg ttacaccttg agttttatcc agcgcaatgt tgatggattc
    13801 ggcaatacgc gcaaggcaat cctcttctga aatctgcatc agatggctgc cagggtggaa
    13861 gttgagcaaa gaaagcccca gttgttcgca acgctgcatt tcatctataa aggcatcgcg
    13921 cgatttttcc agagcttcag tgaccggatg tccgaggttg atcagataac tgtcgtgggg
    13981 aagaatttgc gccgatgtgt agtgatattt ttcacaggcg gctttgaatt catcgatggt
    14041 ttgcgtcgtg agcggtgcgg cacgccactg acgttggttt ttggtgaaca aggcaaacgc
    14101 ggttgcgtcg atttcggcgg cgcgaattgc ggcatttgcc agaccgccag cagcactaac
    14161 gtgcgctcca atgtatttca tgcgaggact cctgttaaac ccgctggagg aaaacggtaa
    14221 tgatagcggg ttaacaggag caagaagtag tggtttatgc gatgacgctt tgaatcacat
    14281 agttaatcgc accaccacca acaatcagcc aggcaaacag taccagtgcc atcagcagag
    14341 gtttcgcccc agcttttttc agcgcgctga cgtgagtggt cagacccagc gccgccatcg
    14401 ccattgccag caggaaggta tccagcgtta ccagcatgtt caccacgctc tgcggtaaca
    14461 ggtggaacga gttaaagatg gcaactacga tgaacaagat ggcaaaccac ggaatagtga
    14521 ttttgctttt ctcgccgctg ttcgccccag acagctgttt aacacgcgcc gccagcagga
    14581 tgaggaacgg agccagcatc atcacgcgca gcattttgga aataactgct gcgttttctg
    14641 catccgggct gatggcatga cctgccgcca ccacctgcgc cacttcgtgc acagtagaac
    14701 caatgtagat accgaaagtt tccggactaa accattgaga catcagcgga tatatcgccg
    14761 ggtagaggaa aatcgcgacg gtcccgaaga taacaacggt tgcaacagcc acggttactt
    14821 tactggcttc cgctttcact accggctcag tcgccagtac cgcggcagca ccacagatac
    14881 tgctaccggc accgatcaac caactggtgt gcttatccag accaaacact ttctgcccca
    14941 ggaagcaagc cagcaggaag gtactggaca gcgtcagcac gtcaatgatg atcccactga
    15001 taccgacatc ggcaatttgc gagaacgtca gacggaagcc ataaagaatg atacccagac
    15061 gtaataaata ttgcttggca aacagcacac caccgtcaca gcttttccag atgtgcggat
    15121 agatggtgtt gcctaaaacc atccccaaca agattgcgag ggtgagggca ctaaacccgg
    15181 cacccgcaac cgcgggaatg gaaccacccc acagggcgac cccggtgata actgcactca
    15241 gggctaaccc cggaataaaa tgccacagtg tacgatgttg tttctgtaag gtgatattcg
    15301 tcataaccct ctcctttaac cgggcataag gttacgacta actggtttaa aaataaaatt
    15361 gattatatat ttataattaa tctttataag tggtaagcga ctatgcacat caccctccgg
    15421 cagttggaag tttttgcaga agtattgaaa agtggatcaa ccacccaggc gtcggtgatg
    15481 ctggcgttgt cgcaatcagc agtgagcgca gccttgactg acctggaagg gcagcttggc
    15541 gttcaactgt ttgatcgcgt ggggaaaaga ctggttgtta atgaacacgg gcggctgctc
    15601 tatccgcgag cgttggcact gcttgaacag gcggttgaaa tcgaacaact gtttcgcgaa
    15661 gacaacggcg cgattcgtat ctatgccagt agtaccatcg gtaactacat tctgcctgca
    15721 gttatcgccc gttatcgcca tgattatccg cagttgccga ttgaacttag cgttgggaac
    15781 agccaggacg tgatgcaagc ggtgctggat ttccgcgttg atattggctt tattgaagga
    15841 ccgtgccaca gcactgaaat catttctgaa ccgtggctgg aagacgagca ggtggttttc
    15901 gccgcgccga cttcgccgtt ggctcgtggt ccggtcacct tagaacagct ggccgctgcg
    15961 ccgtggatcc tgcgtgaacg cggttccggc acgcgggata ttgtcgatta tctgttgctg
    16021 tcacatttac cgaagtttga gatggcgatg gaattaggta actccgaggc aatcaaacat
    16081 gcggtgcgtc atgggttggg aattagttgc ctgtcgcgac gtgtgattga agatcaattg
    16141 caggcaggca cattaagtga agttgcggtc cctctgccgc gcctgatgcg tacgttgtgg
    16201 cgtatacatc atcggcaaaa acacctttcc aacgcgctac ggcgctttct ggactattgc
    16261 gatcccgcaa atgtgccgcg ttaagttgct gtacaagaac atgctggtgc tgtgtcgatt
    16321 tcgtgacgca gcgccttcag catgcattcg ccagaaaaga gattggctgc tttacttata
    16381 atccctgggc gatcatgaag gtgtcttata accgtgtatt tctgccggaa ggattgccaa
    16441 tcgtctgcta caatcgcgcc atcattttta agatggatag catttttgta tgggttccga
    16501 aactaaaact acagaagcgc cgggcttacg ccgtgaatta aaggcgcgtc acctgacgat
    16561 gattgccatt ggcggttcca tcggtacagg tctttttgtt gcctctggcg caacgatttc
    16621 tcaggcaggt ccgggcgggg cattgctctc gtatatgctg attggcctga tggtttactt
    16681 cctgatgacc agtctcggtg aattggctgc atatatgccg gtttccggtt cgtttgccac
    16741 ttacggtcag aactatgttg aagaaggctt tggcttcgcg ctgggctgga actactggta
    16801 caactgggcg gtgactattg ccgttgacct ggttgcagca cagctggtca tgagctggtg
    16861 gttcccggat acaccgggct ggatctggag tgcgttgttc ctcggcgtta tcttcctgtt
    16921 gaactacatc tctgttcgcg gctttggtga agcggaatac tggttctcac tgatcaaagt
    16981 cacgacagtt attgtcttta tcatcgttgg cgtgctgatg attatcggta tcttcaaagg
    17041 cgcgcagcct gcgggctgga gcaactggac aatcggcgaa gcgccgtttg ctggtggttt
    17101 tgcggcgatg atcggcgtag ctatgattgt cggcttctct ttccagggaa ccgagctgat
    17161 cggtattgct gcaggcgagt ccgaagatcc ggcgaaaaac attccacgcg cggtacgtca
    17221 ggtattctgg cgaatcctgt tgttctatgt gttcgcgatc ctgattatca gcctgattat
    17281 tccgtatacc gatccgagcc tgctgcgtaa cgatgttaaa gacatcagcg tcagtccgtt
    17341 caccctggtg ttccagcacg cgggtctgct ctctgcggcg gcggtgatga acgcagttat
    17401 tctgacggcg gtgctgtcag caggtaactc cggtatgtat gcgtctactc gtatgctgta
    17461 caccctggcg tgtgacggta aagcgccgcg cattttcgct aaattgtcgc gtggtggcgt
    17521 gccgcgtaat gcgctgtatg cgacgacggt gattgccggt ttgtgcttcc tgacctccat
    17581 gtttggcaac cagacggtat acctgtggct gctgaacacc tccgggatga cgggttttat
    17641 cgcctggctg gggattgcca ttagccacta tcgcttccgt cgcggttacg tattgcaggg
    17701 acacgacatt aacgatctgc cgtaccgttc aggtttcttc ccactggggc cgatcttcgc
    17761 attcattctg tgtctgatta tcactttggg ccagaactac gaagcgttcc tgaaagatac
    17821 cattgactgg ggcggcgtag cggcaacgta tattggtatc ccgctgttcc tgattatttg
    17881 gttcggctac aagctgatta aaggaactca cttcgtacgc tacagcgaaa tgaagttccc
    17941 gcagaacgat aagaaataag tttcctccct tccttgctaa gccctctcaa ccgagagggc
    18001 tttttcaatt ccatttccct gacaaatcat gcggatataa aatttaatat ttggattgat
    18061 aattgttatc gtttgcatta tcgttacgac acaatcaaaa aaggctgaca aatcagaggc
    18121 tgttccggct ttctgggatg atcacctgca tacaaataag tccaccgcga tgctgccgta
    18181 cgcaagggga cgtaaagaag atgtgagcga tacccatttt attttcgtag ttacctcatg
    18241 gagatatgga atgtttaggt tgaacccttt cgtacgggtc gggctgtgtt tgtccgctat
    18301 ttcttgtgca tggcctgtgt tagcggtcga tgatgatggc gaaacgatgg ttgtcactgc
    18361 atcttccgtg gaacaaaatc ttaaagatgc acccgccagt atcagcgtca ttacccagga
    18421 agacctgcag cgaaaaccgg tacagaatct gaaggatgtc ctcaaagaag tgcctggcgt
    18481 acaactgacg aacgaagggg ataaccgtaa gggcgttagt attcgtggtc tggacagcag
    18541 ctacaccctg attcttgtcg acggtaaacg cgttaactcc cgcaatgccg tcttccgcca
    18601 caatgatttc gatctgaact ggatcccggt cgattccatc gaacgtattg aagtggttcg
    18661 tggcccgatg tcgtcgctat acggctccga tgcgctcggc ggtgtagtga atatcatcac
    18721 caaaaaaatc ggtcagaaat ggtcgggtac cgttaccgtc gataccacca ttcaggaaca
    18781 tcgcgatcgc ggtgatacct ataacggtca gttctttacc agtggaccat taattgatgg
    18841 tgtgctggga atgaaagctt acggcagcct ggcaaaacgt gaaaaggatg acccgcaaaa
    18901 ctcaacgacc accgataccg gagaaacgcc gcgtattgaa ggattctcca gccgcgacgg
    18961 caatgtcgaa tttgcctgga caccgaatca aaatcacgat tttactgccg gatacggttt
    19021 cgaccgtcag gatcgtgatt ccgattcgct ggacaaaaac cgcctggaac gccagaacta
    19081 ctgcgtcagc cataatgggc gttgggatta cggcaccagc gaactgaaat cctacggtga
    19141 gaaagtcgag aacaaaaacc ctggcaacag cagcccgata acttccgaaa gcaatacggt
    19201 cgacggcaaa tacacgttgc cgctgacggc gattaatcag tttctcacgg ttggcggtga
    19261 atggcgtcac gacaaactta gcgatgcggt gaacctgacc gggggaacca gctccaaaac
    19321 gtctgccagc cagtacgcgc tgtttgtgga agatgaatgg cggatcttcg agccgctggc
    19381 gctgacgacc ggcgtgcgta tggacgatca cgaaacctac ggtgaacact ggagtccgcg
    19441 tgcctacatg gtttataacg ccaccgacac cgtaacggtg aaagggggct gggcgacggc
    19501 atttaaagca ccttctctgt tgcaacttag ccctgactgg acgagcaatt cctgccgtgg
    19561 cgcatgtaag attgtgggta gcccggatct gaaaccagaa accagcgaaa gttgggagct
    19621 ggggctttac tacatgggtg aagaaggctg gctggaaggg ggtgaatcca gcgttatcgt
    19681 tttccgtaac gatgtgaaag atcgtatcag catcagccgt acgtctgacg tcaacgctgc
    19741 accgggctac caaaactttg ttggttttga gacgggcgct aacggacggc gcataccggt
    19801 atttagctac tacagcgtta acaaagctcg tattcagggc gtggaaaccg aactgaaaat
    19861 tccgttcaac gatgaatgga aactgtcgat caactacacc tacaacgatg gtcgtgatgt
    19921 cagcaacggc gaaaacaaac cgctatccga tctgccgttc catactgcta acggtacgct
    19981 ggactggaaa ccgctggcgc tggaagactg gtcattctat gtttctggtc actataccgg
    20041 gcagaaacgc gccgacagcg cgacggctaa aacaccgggc ggttatacca tctggaatac
    20101 cggcgcggcc tggcaggtga ctaaagacgt caaactgcgc gcaggcgtgc tgaaccttgg
    20161 cgacaaggat ctcagtcgtg acgactacag ctataacgaa gacggacgtc gttactttat
    20221 ggcagtggat tatcgcttct gatgagaaga tgcccggcga accgggcgga ctttcacttc
    20281 agtaaatact gcgcatggaa gcgcaggtga tcctctataa aagaggcgat gaagtagtaa
    20341 ctgtgatcat atcccggctg aatacgcagc gtcatcggcc acgctttctg gcgcgcggct
    20401 tctgccaata ccgcaggttg caactggtcg gcaagaaact gatcattatc gccctgatcg
    20461 ataagcgtcg ggatcgcatc ctgcgcgtta ctggcataca tcagcgcgca actatcccat
    20521 tccagcatgc atttttgtcc tcacctaaat aggtgctaaa cgctttgatt ccccacggga
    20581 cgctgcacgg attcacaatg ggcgcaaagg ccgaaacgct ggtgtattta cccggatttt
    20641 tcagcgccat aatcagcgca ccgtgaccac ccattgagtg accgctaatg gcgcagcggt
    20701 cgctgacatt aaattgcgac tgaaccagcg ccggtaattc atcgcgcaga taatcataca
    20761 tccggtaatg cgtcgcccac ggcggttgcg tggcattaag ataaaagcct gcgccctggc
    20821 ctaaatcgta gccatcgtcg ttggcaacct tttcgccgcg cgggctggtg tctggcatca
    20881 ccagtacaat ccccagttcc gccgctaccc gctgggcacc cgccttggtg gtgaagttct
    20941 cgtcattgca ggttaatccg gaaagccagt acagcactgg tggcggggtg tgatcacgag
    21001 ggggtgggag aaagatactg aacgtcatcg ggcagtttaa ggtactggag tcgtgtcgcc
    21061 agcgttgctg ccagccttca aaacagcggt gctcttcgag catttccatg cgtggctcct
    21121 tgttgtgttg tttgcaattt cttcatcata ccgtttattc aatgtgctgt gagtaacttt
    21181 cacttccgta tttgcataac gatgttttaa catctgctga tgaaaggcag cggcaattac
    21241 aataattatc gctgtgaata ctggattatg tgcgccgcct cacgcacaat aatcaagctg
    21301 taaatcagct taataacttt gcccccacgc agggcggagg cgtcacacct gcaggagaaa
    21361 tcataaatgc catcactcag taaagaagcg gccctggttc atgaagcgtt agttgcgaga
    21421 ggactggaaa caccgctgcg cccgcccgtg catgaaatgg ataacgaaac gcgcaaaagc
    21481 cttattgctg gtcatatgac cgaaatcatg cagctgctga atctcgacct ggctgatgac
    21541 agtttgatgg aaacgccgca tcgcatcgct aaaatgtatg tcgatgaaat tttctccggt
    21601 ctggattacg ccaatttccc gaaaatcacc ctcattgaaa acaaaatgaa ggtcgatgaa
    21661 atggtcaccg tgcgcgatat cactctgacc agtacctgtg aacaccattt tgttaccatc
    21721 gatggcaaag cgacggtggc ttatatcccg aaagattcgg tgatcggtct gtcaaaaatt
    21781 aaccgtatcg tgcagttctt tgcccagcgt ccgcaggtgc aggaacgtct gacgcagcaa
    21841 attcttattg cgctacaaac gctgctgggc accaataacg tggctgtctc gatcgacgcg
    21901 gtgcattact gcgtgaaggc gcgtggcatc cgcgatgcta ccagtgccac gacaacgacc
    21961 tctcttggtg gattgttcaa atccagtcag aatacgcgcc acgagtttct gcgcgctgtg
    22021 cgccatcaca actgattaaa aggcaggaac catggagcgc aacgtcacgc tcgattttgt
    22081 tcgcggcgtc gccattctgg ggatcctgct attaaacatc agcgcctttg ggctaccaaa
    22141 ggcggcttat ctcaatcccg cctggtacga tgctattacg ccgcaggatg catggacctg
    22201 ggcatttctc gatctcatcg gccaggtgaa attcctcacg ctttttgcgc tgctgtttgg
    22261 tgctggcctg caaatgttgc tgccccgtgg cagactctgg atccagtcgc ggttaacgct
    22321 gttagtcttg ttgggcttta ttcacggttt attgttctgg gacggcgata ttctgctggc
    22381 ttacgggctg gtggggcttg gcgttttgct gttattgggg ctgatttccg acagccagac
    22441 cagccgcgcc tggacgccgg atgcatcggc tattttgtat gaaaaatact ggaagcttca
    22501 cggcggcgtt gaagcgatca gtaatcgtgc cgatggtgtt ggcaacagtt tactggcact
    22561 gggcgcacag tatggctggc aactggcagg gatgatgctc attggtgcgg cgctgatgcg
    22621 cagcggctgg ctgaaagggc agttcagctt acgtcactat cgtcgtactg gttttgtgct
    22681 ggtggcgatt ggggtgacca ttaaccttcc tgccatcgcc ctgcaatggc agctggactg
    22741 ggcgtatcgc tggtgtgcct tcttacttca aatgccgcgg gaactgagtg cgccgtttca
    22801 ggcgattggc tatgcgtcgc tgttttatgg cttctggccg caattgagcc gctttaagct
    22861 ggtgcttgcg atcgcctgcg tcggacggat ggcgctgacc aactatctat tgcaaacgct
    22921 gatttgtacc acgctttttt accacctcgg tttgtttatg cattttgacc gcctggagct
    22981 gctggcgttt gttattccgg tatggctggc gaatatcctc ttctctgtta tctggctgcg
    23041 ttacttccgc caggggccgg tggaatggct ctggcgtcag ttaactttgc gtgctgccgg
    23101 accggcaata tctaaaacat caagataacg atctggatta cattcgttaa caaaacggct
    23161 gtaaccgttt ccattgctgt gactcgattc acgaagtcct gtattcagtg ctgacaaaat
    23221 agccgccagc aagcagtcat ttactgcaat ctcataacag gtagtgaata tgatcaccat
    23281 tcgtgatgta gcgcgtcagg ctggcgtctc tgtggcaacg gtttcccggg tgctcaataa
    23341 cagcacgctg gtcagtgccg acacgcgtga agcagtaatg aaagccgtga gtgagctgga
    23401 ttatcggcca aacgccaatg cccaggcgct ggcaactcag gttagcgaca ccattggcgt
    23461 ggtggtgatg gacgtttctg atgcgttttt cggcgcgctg gtaaaagcgg tggatctggt
    23521 cgcccagcag catcagaaat acgtgctaat cggcaatagc tatcatgaag cggaaaaaga
    23581 gcgtcacgcc attgaggtgt taattcgcca gcgttgtaat gcgttgattg ttcactcaaa
    23641 agcattgagt gacgatgaac tggcgcaatt tatggataac attcccggta tggtgttaat
    23701 caaccgcgtt gtgccggggt acgcccatcg ttgcgtttgc ctggataatc tcagcggtgc
    23761 ccgaatggcg acgcgcatgt tgctgaataa cggtcatcaa cgtattggtt atctttcttc
    23821 cagccacggc attgaagatg acgccatgcg taaagcaggc tggatgagtg cgttgaaaga
    23881 gcaggatatt attccgccgg aaagctggat tggcactggt acgccggaca tgccgggcgg
    23941 tgaggcggcg atggttgaac tgctggggcg caatctacaa cttaccgctg tatttgctta
    24001 taacgacaat atggccgctg gcgcactgac agcattaaaa gataatggca ttgcgattcc
    24061 gttacatctc tcaatcatcg gtttcgatga tattcccatc gcccgttaca ccgacccgca
    24121 attaacgacc gtgcgttatc ccattgcttc aatggctaaa ttagccaccg aactggcctt
    24181 gcagggggca gcaggcaata ttgatcctcg tgccagccac tgttttatgc cgacgttagt
    24241 gcgtcgccat tctgtcgcaa cgcgccagaa tgcggcggcg atcactaact caacaaatca
    24301 ggcgatgtaa ccgctttcaa tctgtgagtg atttcacagt atcttaacaa tgtgatagct
    24361 atgattgcac cgttttaatg ttgtaacccg tatgtaacag tgaataatca ctttcaccga
    24421 ggtaatagcg tcataacaac aattaaagcc gttttctgga gcgttaccgg gcatggaaga
    24481 acgaatttta aaaagtgagc ttcggcgttc agtaacactt cattaactct actgccccgc
    24541 cgagcattta tctcaagcac taccctgcat aagaaaaacc ggagatacca tgaataagaa
    24601 ggtgttaacc ctgtctgctg tgatggccag catgttattc ggtgccgctg cacacgctgc
    24661 tgatactcgc attggtgtaa caatctataa gtacgacgat aactttatgt ctgtagtgcg
    24721 caaggctatt gagcaagatg cgaaagccgc gccagatgtt cagctgctga tgaatgattc
    24781 tcagaatgac cagtccaagc agaacgatca gatcgacgta ttgctggcga aaggggtgaa
    24841 ggcactggca atcaacctgg ttgacccggc agctgcgggt acggtgattg agaaagcgcg
    24901 tgggcaaaac gtgccggtgg ttttcttcaa caaagaaccg tctcgtaagg cgctggatag
    24961 ctacgacaaa gcctactacg ttggcactga ctccaaagag tccggcatta ttcagggcga
    25021 tttgattgct aaacactggg cggcgaatca gggttgggat ctgaacaaag acggtcagat
    25081 tcagttcgta ctgctgaaag gtgaaccggg ccatccggat gcagaagcac gtaccactta
    25141 cgtgattaaa gaattgaacg ataaaggcat caaaactgaa cagttacagt tagataccgc
    25201 aatgtgggac accgctcagg cgaaagataa gatggacgcc tggctgtctg gcccgaacgc
    25261 caacaaaatc gaagtggtta tcgccaacaa cgatgcgatg gcaatgggcg cggtagaagc
    25321 actgaaagca cacaacaagt ccagcattcc ggtgtttggc gtcgatgcgc tgccagaagc
    25381 gctggcgctg gtgaaatccg gtgcactggc gggcacagta ctgaacgatg ctaacaacca
    25441 ggcgaaagcg acctttgatc tggcgaaaaa cctggccgat ggcaaaggtg cggctgatgg
    25501 caccaactgg aaaatcgaca acaaagtggt ccgcgtacct tatgttggcg tagataaaga
    25561 caacttggct gaattcagca agaaataaga ctgattgggc gcagtcgttt actgcgccct
    25621 tattgtatgg ccaacaaggt aaaattatat ggtcagctca acgactccgt cttccgggga
    25681 atacttgttg gaaatgagcg gtatcaacaa gtcctttcct ggtgttaagg cacttgataa
    25741 cgttaattta aaagtccggc cacattctat ccatgcatta atgggggaaa acggtgcagg
    25801 aaaatcgaca ttattaaaat gcctgtttgg tatttatcaa aaagactccg gcaccatttt
    25861 attccagggt aaagagatcg atttccattc cgccaaagag gcactggaaa atggtatttc
    25921 gatggtacac caggagttaa acctggtatt acaacgttcg gtgatggata acatgtggct
    25981 ggggcgatat cccaccaaag gcatgtttgt cgatcaggac aaaatgtacc gcgaaaccaa
    26041 agcgattttt gatgaactgg atattgatat cgatccgcgt gcgcgcgtcg gcacattatc
    26101 cgtttcgcaa atgcagatga tcgaaatcgc caaagcgttt tcctataacg cgaaaattgt
    26161 gattatggat gaaccgactt cttcgttaac cgaaaaagag gtcaatcatc tgttcactat
    26221 tattcgtaaa ttaaaagagc gcggctgcgg tattgtttat atctcgcata aaatggaaga
    26281 aatcttccag ttatgtgatg aagttaccgt attgcgcgac ggtcagtgga tcgccaccga
    26341 accgctggca ggactgacga tggacaagat catcgccatg atggttgggc gttctcttaa
    26401 tcagcgtttt cctgacaaag aaaacaagcc gggcgaagtc atcctcgagg tgcgtaacct
    26461 aacgtcactg cgccagccgt cgattcgcga tgtctcgttt gatctgcata aaggggagat
    26521 cctcggtatt gccgggctgg tgggggcgaa acgtaccgat attgttgaga cgttatttgg
    26581 tattcgcgag aaatcggctg gcaccattac gttgcacggc aaacagatca ataaccataa
    26641 tgccaacgaa gccataaacc acggatttgc actggtaact gaggagcgcc gctcaacggg
    26701 aatttatgcc tatctggata ttggttttaa ctcgttaatt tccaatattc gcaactacaa
    26761 aaataaagtt ggtttactgg ataactcgcg gatgaaaagc gatacccagt gggtgattga
    26821 ttcgatgcgg gtaaaaacgc cgggtcatcg gacgcaaatt ggttcgctct ccggtggtaa
    26881 tcagcaaaag gtgattattg gtcgctggct actaacgcaa ccagaaatat taatgctcga
    26941 tgaaccgacg cgcggtattg atgtcggggc gaagtttgaa atttatcagt taattgccga
    27001 actggcgaag aaaggcaagg ggattattat tatctcctct gaaatgcctg agttgttagg
    27061 gataacagac cgtattctgg tcatgagcaa tggtctcgtt tccggaattg tcgatacaaa
    27121 aacaacaacg caaaacgaaa ttctgcgtct tgcgtctttg cacctttaag attaggggct
    27181 tcccatgagt gcgttaaata agaaaagttt tcttacttac ctgaaagagg gcggtattta
    27241 cgtcgttctt ttagttttgc tggcgattat tattttccag gacccaacat ttttaagtct
    27301 gttgaactta agtaatattc tcacccagtc atcggtgcgt attattatcg cgctcggtgt
    27361 ggcagggtta attgtcaccc aggggaccga tctttctgct ggtcgtcagg tagggctggc
    27421 ggcagtggtg gctgcgacat tattgcagtc catggataac gccaacaaag tgttcccgga
    27481 aatggcgacg atgccgattg cgctggttat tctgattgtc tgtgccattg gtgcggtgat
    27541 cggtttgatc aacggtctga ttatcgctta tctcaacgtg acgccgttca ttaccacgct
    27601 cggcacgatg atcatcgtct atggcatcaa ctcgctctat tacgactttg tcggggcgtc
    27661 gccaatttct ggttttgaca gtggcttctc tacctttgct cagggctttg tcgcgctggg
    27721 gagtttccgt ctctcttaca tcaccttcta cgcgttgatt gcggtggcgt tcgtctgggt
    27781 gttgtggaac aaaacccgct tcggtaagaa catttttgcc attggcggta acccggaagc
    27841 ggcaaaaata tctggtgtca acgtcggcct gaacctgctg atgatctacg cgttgtctgg
    27901 cgtgttctat gcctttggcg ggatgttaga agccggacgt atcggctctg ccaccaacaa
    27961 cctcggcttt atgtatgagc tggatgctat cgcggcgtgc gtggtaggcg gtgtatcgtt
    28021 cagcggcggt gtggggacgg tgattggcgt ggtgaccggg gtaattattt ttaccgtcat
    28081 caactatggc ctgacgtata tcggcgtaaa cccatactgg cagtacatca tcaaaggggc
    28141 gattattatc ttcgccgtag cgctggattc actgaaatac gcgcgtaaga agtgatgtga
    28201 ttgcggatat tgccggatgc gtctctggtt ttacaacgtt accgggtgtt ctttctcgcc
    28261 tttcttaaac ttcacttccc cgagctcaat acaacccacc gggcagacgt gaccacaaag
    28321 cagacaaccc acacatttct cggtattaca atgcggtgtg cgggttttct cgctccattc
    28381 catcgcctgg tgaccgccgt cgtaacagga aatataacag cgtccacagc caacacattt
    28441 gtcaagattg atacggggat agacaatata actgcggtct aaatcttccg ccgggacaat
    28501 attgttattc gccagaccta ccatctcctg caacgaatca aatccctgat cggcgagata
    28561 atgcgataac ccgctcgcca tatcttctac gatgcgatag ccgtactgca taatgccggt
    28621 ggtgacctgt aacgttgctg cgccgagcaa taaaaactcc gccgcatctt cccaggtttc
    28681 aatgccgccg ataccgctga ttgggaaatc gcgcagttct ggatgggtgc gcatttgctg
    28741 aatgaagcgc agggcgatcg gttttaccgc tttaccggaa tatccggaaa tactcgattt
    28801 accgttaacg atcggcatac cgattttctg attaagatcg atattggtga tggatttaac
    28861 ggtgttaatt gccgcaatgc catctgcgcc gccgcgcttc gccgccagcg ccacttcgca
    28921 catatcaccg atattcggcg tcatcttcgc cagcattggc agcgtggaac cccgtttcac
    28981 tgcccgacaa tatttttcta ccagctccgg gctttgcccg acatcgctac ccatcgcatg
    29041 agaagtcatt tgcggacagg agaagttaca ctcgatcata tccgcgccag ctgctcccat
    29101 tgctgctcat tttcccccat gatcgaagcg atcaatactt tgtccgggta atcttccttc
    29161 agccgacgca gggcggtcag attctcttcc aacggatgtt cagcaatctg ctccatattt
    29221 ttgaagccga taaaaccggt atcttctttc accagatgat caaaacgcgg cgagacttcg
    29281 ttggcgataa aaaagccgat cgttttaaac accacaccgc cccaacctgt gtcgtaggct
    29341 ttggcacaca tctcatagca gttgcctacc ggcgaagaag agaggcagaa cgggttggga
    29401 aagttcacgc cgcaaaaagt aatcgaaaga tctttcgtta acatgagcaa gctccctcta
    29461 aatagtgatg aatcgcctcg gcggcttctt tcccggtttt cacggcatag accacggttt
    29521 tgtcaccctc aacaatatcg ccagcagcaa agacttgcgg atcgcgggtc tgataatttt
    29581 gtgttttgat ggtgttacgc tgctgctcta actccgcaaa ggcatccagt ctggcatgct
    29641 gaccgacggc gagaataatt ttatctgccg ccatcgtcag ttcgcctgat aaccgtacat
    29701 gcttaaacgt gactttattg ccttccacgg ctactggcgt gaatccatcg atgatcgaaa
    29761 cgcccagctc ccgggcgctg gcaaactctt tttcgcttgc cggaaactca tctaactctt
    29821 cacgtgctac gcaagttacc gcctgacagc cgagaacttt cagcgtgctg gctacgtcca
    29881 tcgcgacatc accgccgccg ataattaatg cgctttgtgg aatgctgata tcgccttgcg
    29941 cctgtcgtac acgttgcagg aagtcgacgg caatctcaac gtcgctatgc tcaaacagcg
    30001 gtagcccgga accgcttgat aaccccacgg tgaccagtac cgcgcggttt tccgctttca
    30061 gttgctccag ggtgagtgtt ttaccgattt cgttattgca cttaatggtc acgcccattt
    30121 tttcaatacg ggcgatctct gcatccagca ctgattgcgg taaccggaat tgcggaatac
    30181 cgttacgcaa ccagccaccg gggtgcgctt ctttctcata aatcgtgacg tcataaccct
    30241 ggtttgtcag tgtcacactg gcctgcaatc cggcaggacc tgcgccaata atcgcgactt
    30301 tgccgagcgt tttagtaccg ggctgataaa tttccatccc ggtttgttgt tcaaaatcag
    30361 taacaaaacg ctgtaagcgg ccgatatcaa tgggagcatc aacaccggca cgggtacaac
    30421 cgctttggca taatttctcc gtcgggcaca ctctggcaca aacggcaccg agggcattat
    30481 tttcgcgaat tgtctcggca gcgcctttaa aattacgaaa gtagattgag cgaataaatt
    30541 tccccggatc ggtctgtgcc gggcaagcct gactacaggg agcgtcgtga cataataaac
    30601 agcgagaggc ttctttaatc gccagtaacg gcgtaaaagc cggagtgagt tcatccagat
    30661 aattttgttg cggcatgtga tcttccttaa tgctatgagt aattatggcg aacgctaaat
    30721 aaatattgcg tgctgatatt aaggctatgc gcgtggggaa atttaagctg tgaaaggatt
    30781 cacaaatagc tgcggttcag aatgttatat ccgcagctta ttgagtgaag taataattat
    30841 tctcaataat taacagaaaa gagaaaatat taacgataga aaataagata aacaatcatg
    30901 ccgatagata aaattccgaa gccaatatat gccaccatat aagcgaagaa gagtccggga
    30961 ttttttgctc gccagacata caccggttcc ggtgcatttt taacccgttt atcaatcgcg
    31021 cctgcgcgcc agcctttcca tgcttcgcgg aaacaaaata tcgggataag gaaaaaaacg
    31081 gcaacgacaa aaaactgctg tacatccata actactttcc ttgtttcttt tgtaattcaa
    31141 gtaactgttc gggtgtaacg gcgggatacc cctgcgcatc ttccggcacc tggtgcatag
    31201 ccggaatagg gaccagcggc cctaaaaaac gcggttcacg tttaaaaata taaaggtcag
    31261 ccagcgcacc gaaacgggcg gcaaattcgc gaatacgtac tgacagcata tctttcggtg
    31321 acggtacggc ataacactga gcctgaatcc ccatatgcag cgcaataaat aatgctcgct
    31381 cacagtggaa acgttgggtg ataataatga aatcattagt atcgaaaact ttgcgtgtac
    31441 gcacgatgga atccagcgta cgaaagcctg cgtaatcgag aacaatatct gatgggtcga
    31501 caccagcagc gattaaatct ttgcgcatgg tcatcggctc gttataactt tgcaatgcgt
    31561 tatcgccgct cagtaataga taatttacct taccgctgtt ataggcatta atcgctcctt
    31621 gaatgcggta gcgataatac tgattaataa cgccggtacg ataatatttt gctgtgccga
    31681 gcaccacgcc gacctggcgg taggggagat cctgcaattc gtcgtagata taaggcgcgg
    31741 ttttccagct catccagcga tcgaggccga gcacagtcaa cagcagcaag ccgatcagga
    31801 ctaacaggct gaggaacacg cgctttaaca tgcaggagac tcgagctaag gaaaatttcc
    31861 atcaggctac tttacccgct ggttttgcgc aagaaacggc aaactgtttg ccgggaattt
    31921 cgacaccagg agagggctgg caagccggac aaggcgttca cgccgcatcc ggcaccaggc
    31981 ttaagcgaga agcacgcggt cgatactgtg acagccgaga gctttcagcg ttgcggaggt
    32041 ggcatcccac tgaatcaacg gcgcatcggc tttttctgcc agaaccgcgc gctggatatc
    32101 cgggtaatca taacccttga gattcaacag aattaacgcc ccctgcaacg gcggcagagt
    32161 cgggttgaat gcggcgtttt cagcgtagct gccgctgaaa atacgaccgt ctttacattc
    32221 cagcgcgaca ccgcttggtg acttactgta aggcatgtgc gaacggtttg ccgctgcaat
    32281 cgccgcctga gaaagcgcat caccagtcag cgcatagccg tgatcctgct cgtccatcag
    32341 cagcgtttta atctccagat ctttcggccc aaaggcatcc ggcagatagt cacgcagcgc
    32401 gtgtgcctcg cggcccggca aatgaatacg cagatccaga ccgctgttca gttcattcat
    32461 aaactgacgg cagtgaccac aaggcgtata gttaacggtg atggctgcaa gcgctttttc
    32521 accactcaac caagcgtggc tgattgcgct ttgttcggca tgaacggttt gctgcattgt
    32581 tgcaccaata aattccatat tggcaccaaa ataccaggtt ccgctcacac cgcgcgcaat
    32641 tgcgccaaca ttaaaattcg acaatggcgt acgcgcacag gccgccgcca gcggaagtag
    32701 tgcgaatgcc agcgcgtctt cgtccagtcc cgttgcgctc ttcagcgatg agacttgctc
    32761 cccggtcaac aaagcgggga agtacttgtc tgccagaata ggttccagtg cagattgcaa
    32821 gttatccgca agttgggcaa aagcggtttg aaaacgtgga tgcatggcgt tgcctcataa
    32881 tcatgtaatg ggataacagt ttacgaaccc gttatggctt tatatgtgat ctgaatctca
    32941 ttaattatgc aacacgtgaa acttacatca aaaatgcgcg acgtatcgca aattttagcc
    33001 cattactgcc agaataatcg ggaaaaggaa cggtgcgatc agcgaagtaa ttatcccgca
    33061 taacaccagc gctagcgaac taaatgcacc ttcctgataa tccagctcgg cgcagcgcgc
    33121 cgtaccgagg gcgtgcgagg cagtccccat tgccagaccg cgcgcagctt tggtacgaat
    33181 acgcatcgca ttaagcaatg tatggccaaa taccgcgccg aggatgccga cgaaaatcac
    33241 gcaaactgcg ctgattgccg gaataccgcc aatactgccg ccaaccgcca ttgcaatagg
    33301 cgtggtgact gatttcggca ggattgacgc ggcgatttcc ggtgaagcgc ccatcaacaa
    33361 tgccacggaa gtccccgtca ccatcgcaac cacgctgccg ataaaacaga tggtgatgat
    33421 cgatttccag cgcgcgcgga tctggtgtag ctgctcatat aaaggatagg ccagcgcgac
    33481 gaccgccggt tgcagcagat cgtttagcac ttcgctacct ttaaagtaac tgtcgtaaga
    33541 gatgccggtc agcattaaaa aaggaatgat caccaccatt gctaccagca acgggttaag
    33601 caggggaaac ttataccgtg ccgccagttt gcgggcggca aaaaagacaa tcaacgtcag
    33661 cggtaatgac caccagatat tcgccatcat tcttctgatc ctttctgacc tacgacttta
    33721 cgttcaccgt gtaccagttg tgaactccag ctcaccacca gaaaaaccac cagcgtactg
    33781 acagcacagg aaaccactac cgggccaaac tgcgcgcgga gcaaatcaaa atattgcatg
    33841 acgcctacgc caatcggcac aaacaatagc gccatatagc gaatcagtac gtagcacccc
    33901 ggattgaccc atttcgccgg aagaatttgc aaggccagca ggacaaacag gatctgcatc
    33961 ccgatgatgc tgcccggaat ggttaccggt agcagggaag caatgaaaat gcctgcatac
    34021 aggcaggcat aaatcaggat gaaagcgcgt aaatattgcc agataatgtt cagtgtctta
    34081 ctcataatca acaccccaga tgagatggat tcatcataca attaattcgt aaaacgtgct
    34141 accgatcaca ttaagaaatc agcgcttatt ttttcgctac ccggtattac gcaatgagca
    34201 gcgaactcag gcatacatca agcatagaga gggattgcag tttgcagcaa tccctctttg
    34261 cagaccttta atccgctgac cataggcgct aacatagcgc ctcatttttt tgcgggtgat
    34321 gatatgcgtg tgttactggc accgatggag ggagtgcttg actctcaggt gcgtgaattg
    34381 ctgaccgaag ttaacgacta cgatctgtgc atcaccgagt ttgtccgcgt ggtggatcaa
    34441 ctgctgccgg taaaagtctt tcatcgcatt tgccctgagc tacaaaacgc cagccggaca
    34501 ccatctggta cgctggtgcg cgtgcagttg ttaggtcagt tcccacaatg gctggcagag
    34561 aacgccgccc gtgcggtgga gttaggtttc tggggcgtgg atctcaattg cggctgcccg
    34621 tcgaaaacgg ttaacggtag cggcggcggg gcgacgttac tcaaagatcc tgaactcatc
    34681 taccaggggg caaaagcgat gcgtgaagct gtaccggcgc atttgcctgt cagcgtgaaa
    34741 gtgcgtctgg gctgggacag cggtgagaag aaatttgaaa tcgccgatgc ggttcaacag
    34801 gctggcgcta cggagctggt ggtgcatggg cggacgaaag agcagggtta ccgcgcggag
    34861 catattgact ggcaggcgat tggcgagatt cgccagcggc tgaatattcc ggtgattgcc
    34921 aacggtgaaa tctgggactg gcagagcgcg caacaatgca tggcgatcag cgactgcgac
    34981 gcagtgatga ttggtcgcgg ggcgttcaat attcccaacc tgagccgggt ggtaaaatat
    35041 aacgaaccgc gaatgccgtg gccggaggtg gttgctttgc tgcaaaaata tacccgtctg
    35101 gaaaagcagg gcgataccgg gttatatcac gttgcgcgga ttaaacagtg gttgagttat
    35161 ttgcgtaaag aatacgatga agcaacggaa ttatttcagc atgttcgggt gttgaataac
    35221 tcccctgata ttgcaagggc tattcaggca attgatatcg agaaactcta aaaaccatag
    35281 tgatgttttt gacaatgtgt tagctcttta tcaataggcc gcacggttgt ttggtggcgt
    35341 caaaatcgcc gaggcgttcc ctgaaggccg gggcagccca catggatgtg ggctgagggc
    35401 gcgttttaca gggatgttac ctcgcgcccg acccggtagc cgtaagggat aagccgaggg
    35461 caccgcgcag cggcgatttt gttcgccaga gcccgggggg gcagggggcg gcggcgattg
    35521 gtcgccccct gcgcgctcct tgcgccagtg attatatgaa gcaatgcaca tgacaggagc
    35581 gcaacaggat gagagactga aatattctcg ttattttcgg atctgatgac attaattaat
    35641 tttaaaatat catcttaaat acgccagtca ccaccagtag cccaatcagg aaaataatca
    35701 atacagccca gagtataatt ttcattggcg ttcctttatt tgtgagtgct gaataagtat
    35761 aggaaaaaac gaagtccttt gccgttatcc ataaacgatt aagataaatc ggaagatatc
    35821 atttacttag tctatatttt tctgggtatg tcgttttatt gctactgatg tattgccacg
    35881 cgaaactaaa aaatatatcg caacacacgg taatctccgc gcaccgtttc ttacctgatt
    35941 attcccccat gaatcgtgat tccttttatc cagccatcgc ctgttttccg ctgttactga
    36001 tgctggccgg gtgtgcgcct atgcatgaaa cccgccaggc gttaagccag caaacgcccg
    36061 ctgcacaagt tgacaccgca ttacccacgg cgctgaaaaa tggttggcca gacagccaat
    36121 ggtggctgga gtatcacgat aatcagctca cttcattaat taacaatgca ctacagaacg
    36181 caccggatat gcaggtggct gaacagcgca ttcagctagc tgaagcccag gcgaaggcag
    36241 ttgccactca ggatggtccg cagatcgact tttcggcgga tatggagcgg caaaaaatgt
    36301 cggcagaagg gctaatgggg ccgtttgctc tgaacgatcc ggccgcaggt acgaccggtc
    36361 cgtggtacac caacggaact tttggcttaa cggcgggctg gcatctcgat atctggggaa
    36421 agaatcgggc ggaggttact gcccgcctgg gtacggttaa agcacgggcg gcggaacgcg
    36481 agcaaacccg ccaattgctg gctggcagcg tagcccgcct gtactgggag tggcaaaccc
    36541 aggcggcgtt aaacacggtc ttgcagcaaa tagaaaaaga gcagaacacc attatcgcga
    36601 ccgatcgcca gctatatcag aacgggatta cttcttcagt tgacggtgtg gaaaccgata
    36661 ttaatgccag caaaacccgg cagcagctca acgatgtcgc ggggaaaatg aaaattattg
    36721 aggcacggtt aaatgcactc acaaatcacc agactaagtc attgaagctt aaaccggtcg
    36781 cgttgccgaa agtggcaagc cagcttcctg atgaactggg gtactcctta ctggcccggc
    36841 gggcagattt gcaggcggcg cactggtacg ttgagtcatc gttaagcact attgatgcgg
    36901 caaaagcggc attttatcct gacatcaacc tgatggcctt cctgcaacag gatgcgttgc
    36961 acttaagcga tctgttccgt cattccgcgc agcaaatggg cgttacggca ggcctgacgc
    37021 tacctatttt cgatagtggt cgtcttaacg ccaatctcga tatcgcaaaa gccgaaagca
    37081 acttgtctat cgccagctac aacaaagcgg tggttgaagc agtgaatgac gtggcgcggg
    37141 cagccagtca ggttcagaca ctggcggaga aaaaccagca tcaggcgcaa attgagcgcg
    37201 atgccttgcg tgtggtaggt cttgcgcagg cccgctttaa cgcgggcatc attgctggtt
    37261 cccgcgtcag cgaagccaga atcccggcgc tgcgtgagcg ggccaatggc ctgttattgc
    37321 aagggcagtg gctggatgcc tccattcgac tcaccggtgc gctgggcggg ggttacaaac
    37381 gctgatgata tgcgccttct atacttaacg tttattcagc gttaagtgga gaactcgatg
    37441 gcataggtcg cgataattac cgcttccgat tcggggatcg gcaaagagtg cgcgttatta
    37501 ctggcgcagc aggggtttga tattggcatt acctggcact cagatgaaga aggggcaaaa
    37561 gataccgcgc gtgaggtggt tacccacggc gtacgtgcgg agatcgtgca actggatctc
    37621 ggcaatctgc cagaaggtgc gcaggcgctg gagacactca tccagcgatt tgggcgtatt
    37681 gatgtgctgg tgaataatgc gggtgcaatg accaaagcgc cgtttcttga tatggctttt
    37741 gatgagtggc gcaagatttt taccgttgat gttgatggtg catttttatg ctcgcaaatt
    37801 gcggctcgtc agatggtgaa ataagggcag ggcggtcgca tcatcaacat tacgtcggta
    37861 catgaacata cgccgctgcc ggatgccagc gcctacacag ccgctaaaca tgcgctcggc
    37921 gggttaacca aaacgatggc gctggagttg gtcaggcata agattttggt gaacgcagtc
    37981 gcgcctgggg cgatagccac gccaatgaac agcatggatg atagcgacgt gaagcccgac
    38041 gcggagcctt cgattcctct acggcgtttt ggcgcaacgc atgagattgc cagcctggtg
    38101 gtgtggcttt gttcggaggg cgcaaattac acgacagggc agtcgttgat agtggatggc
    38161 ggctttatgt tggcgaatcc gcagttcaac ccagaatagc gcagtgaggt gcgtggatat
    38221 ttcaatgggt taggccagat aagacaccag cgtcgcatca ggcatggaac acccggtgcc
    38281 ggatgtggag tgaatgctta tctgtggcaa ctgctttacg cctgattatc cggctttttc
    38341 ttcccgcgcc gtttcagcca ccagcgcacg cccaccacca gaaccacgac cagaatcaac
    38401 cagacccagt gcttcaaatg ctggtcgaga ttgtgcaacc acggcgcaat cacctgacca
    38461 ccagcgtaac caatagtggt aaaaatcaac gcccaggcaa atgcgccgag aatattcagc
    38521 ggcagaaaga ttttcggcgg cagctggctg gcaccaatca gcgtcgggcc aatcacccga
    38581 aagccataca taaagcgcgt accaatgaca aacagatacg gatggcgttg gataagtttc
    38641 tgtgcccgct caattttatc ctgatgtttc gagaaacggc gtaacagctt gccgccaaac
    38701 cgccgcccgc acagatagag cacctggtcg ccaatcatgc caccaagcgc cacagaaagt
    38761 accaccagtg ggaactttaa tagcccctga tgtgccgcaa cgcctcccag caaagtcacg
    38821 gtttcacctt ccgccaggct accgataacc agcgcggcat aaccatattg tgagatgagt
    38881 gtattgagag ccataggcca gtttgtctcc ctttggttgc ctgtaagcat acaccctgtg
    38941 aatcattcac gctgaaaaag gacatacatc agacggtgca aattacatgg atgaacaaaa
    39001 aataatcaca agctggctta tacttgaact gtcaggttca tgaactcaca acaagggggc
    39061 ggtatgagcc atgtctgggg actgttttcc catcccgatc gtgaaatgca ggtgattaat
    39121 cgcgaaaacg aaacgatttc tcaccattat acccaccacg ttttgctgat ggcggcgatt
    39181 ccggtgattt gcgccttcat tggcactaca cagattggct ggaattttgg cgatggcact
    39241 atcctgaagt tatcctggtt taccggactg gcgctggctg tcttgtttta cggcgttatg
    39301 ctggctgggg ttgcggtgat ggggcgggtc atctggtgga tggcgcgcaa ttacccgcag
    39361 cgtccgtctc ttgcgcactg catggtcttc gcgggctacg ttgcaactcc gctgttttta
    39421 agcggtctgg tggcgcttta tccactggtc tggttgtgtg cgctggtcgg cacggtggcg
    39481 ctgttttaca ccggctacct gctttacctt ggtattccga gcttcctgaa tatcaacaaa
    39541 gaggaaggcc ttagcttctc cagttcgacg ctggcgattg gcgtgctggt actggaagtg
    39601 ctgctcaccc taaccgttat actatggggc tacggttatc gtttgttcta gaaagttctt
    39661 tgacgttgca ttgctggcgt aaatgtcagc aatgcagcat ttactcacga ttctcctttg
    39721 acgatcgcac tttttgctcg ttatgatggc gtcgctagcc tcagtaaatc cttactccgg
    39781 cggtgcgcaa cccgtgcgcg tgaaccacta tctgaatgct catcatgccg aaatttcgag
    39841 tttctttatt tagcttggcc ctgatgctgg ctgtgccttt tgcaccgcag gccgttgcga
    39901 aaacggcagc cgctaccacc gcttcacaac cggaaattgc ctccggtagc gcgatgattg
    39961 ttgatctgaa taccaacaaa gtgatctatt cgaaccaccc ggatctggtg cgtccgattg
    40021 cgtctatcag caaattaatg accgcgatgg ttgtgctgga tgcacgactg ccgctggatg
    40081 aaaaactaaa agtggatatc agccagacgc cggagatgaa aggggtctat tcgcgcgtac
    40141 gactgaatag cgaaatcagc cgtaaagata tgctgttgct ggcgctgatg tcttcagaaa
    40201 accgcgcggc ggcaagcctt gcgcaccatt atcccggtgg ttacaaagcc tttattaagg
    40261 caatgaatgc gaaagcgaaa tcgctcggaa tgaacaacac gcgctttgtt gaacctaccg
    40321 gattgtcggt gcataacgtt tcaactgccc gtgacttaac caaactgctc attgccagca
    40381 aacaatatcc gttgatcggg cagttaagta ctacccggga agatatggca accttctcta
    40441 acccgacgta tacgctgccg ttccgcaata ctaatcatct ggtgtatcgc gataactgga
    40501 atattcagtt aaccaaaacc ggctttacca atgcggcggg ccattgtctg gtgatgcgta
    40561 cggttatcaa taataaaccg gtggcgctgg tagtgatgga cgcgtttggc aaatataccc
    40621 attttgccga tgccagccgc ctgcgtacct ggattgaaac cggtaaagtg atgcctgtgc
    40681 cggcagcagc gttgagctat aaaaaacaaa aagccgccca aatggcggcg gcggggcaga
    40741 cggcacagaa cgattaagtg aattcggatg gcgatactct gccatccgta atttttactc
    40801 cacttcctgc cagtttttcc gtttactggt tttaccgatc cccggattca tgctattggt
    40861 cggatcattc tcgcgataga acttctgcaa cgtctccggt gctttataca aatgaccgac
    40921 gttatgctcg gcagggtact gcgcgccgcg ctgctgtagc agttccagca tctgttcttt
    40981 taacgcatgc acatccacgc cttttttcac tatgtaatcc tgatggaaga catagcacat
    41041 aaaatggccg taatagagct tgtgcaccag ctggctgtcg atctccggcg gtaaatgctc
    41101 ataccactcg gtgtcgttac gccgcagagc gatatccagt gccagaatgt cttcgacttc
    41161 atcggaatgc accgcctgat aacgaattgc tgcgcccgca gcggcgaaac ggtgtaaaaa
    41221 cgctttgcta ccttcctccg gcgtacagac aaagaaatcg ccctcggcct gtttgaaata
    41281 atccaccagc cacgatttgg cttcaccgac gccatcgcct gccattttta acagcagatg
    41341 atgctcgtat ttatcgcgcc agtttttcat gcgcggcggt aaatggctgg ggaacaggtg
    41401 accgaatttt tgcattgcac ggtcagtaaa atgcggacgg aagaatttca ctttctccag
    41461 catcgcatcg gtgcgtccct tgagattaaa gaagaacggc atcttgtcgg tgcctaactt
    41521 atcaatcatc aggaaggtgt ctttgccgta tttttccgca atatcgtaga tatcccggtg
    41581 catatattcc ccggcaaccg gcagattttc gaagttagcc agaatatgac ggcggatttc
    41641 ggtcagcact tccggctggt tggtgccgat ataaaacacc tgctgatttt tttccgcttc
    41701 gaaggtatca agacgtaccg caaagacggc cagcttcccg gcgcaaccag aagattcaaa
    41761 taaccgatcc ggatcggcgt tataacgtgc gggcgtgtcg gcttcaatat cacgaacgcg
    41821 gtggacatag tcataatcgt gggcgtgacg accatcgtga cgcacatcgt catctttgat
    41881 gcgatcgtca tccagcttgc taaggatctg ctccggcgtt tcgcccagat caatccccag
    41941 atggttcacc agcgtcagtt tgccatcttc atctatacgc gcgaataacg acatttcggt
    42001 atacgccggg ccacgttgca ccagcgagcc gccggagttg ttacaaatac cgccgatgac
    42061 cgatgcgcct atacacgatg atccaatcac cgaatgcggt tcgcgtccca gcggtttgag
    42121 ggctttttcc agcgaataga gcgtggtgcc cggataggcc agcacctgtt cgcccttgcc
    42181 gagaacgtgc agcttgtcga gacgcagggt gctgatgatc acgatatcgc gatcatagtc
    42241 gttaccgttt ggcgtcgatc cttcggtcag gcctgtattg gcagcctgca tcagaataat
    42301 tttgtcggcg gtgacgcagg ctttcagcac ccgccacaat tccagtagtg agccagggaa
    42361 aacgacagcc agcgcgtcgc cctgaccaga acggaagccc ttgcgatagc gggccgtttt
    42421 tgcgggatcg gtgagcaggt gtgaatgacc gaccagacgg gcaagttcat tcaaaaaggc
    42481 tttattatca gttgttgtca tggaagacat tttccactcc ttgtggtggc gaaaaaatat
    42541 cacaaactag aatagcgcct tgtatgacaa ttcagcgagt gaatattttg aaaattcaga
    42601 caataaactg agcattcttt ttctctctcc atcatgctta tggcaaacta cggtttttgc
    42661 accattttcg ctgccacgtc gggcaacaaa ggaagaaaaa tccatatgaa atggctatgt
    42721 tcagtaggaa tcgcggtgag tctggtcctg cagccagcac tggcggatga tttattcggt
    42781 aaccatccat taacgcctga agcgcgggat gcgttcgtca ccgatttgct caagaaaatg
    42841 acggttgatg agaaaattgg tcagctgcgc ttaatcagcg taggcccgga taatccgaaa
    42901 gaggcgatcc gcgagatgat caaagacggt caggttgggg cgattttcaa caccgtaacc
    42961 cgtcaggata tccgcgccat gcaggatcag gtgatggaat taagccgcct gaaaattcct
    43021 cttttctttg cttacgacgt gctgcacggt cagcgcacgg tgttcccgat tagcctcggt
    43081 ctggcctcgt cttttaacct cgatgcggtg aaaacggtcg ggcgtgtctc tgcttatgaa
    43141 gcggcagatg atggcctgaa tatgacctgg gcaccgatgg tcgatgtctc gcgcgatccg
    43201 cgctggggac gtgcttccga aggttttggc gaagatacgt atctcacctc aataatgggt
    43261 aaaaccatgg tggaagcgat gcagggtaaa agcccggcag atcgttactc ggtgatgacc
    43321 agcgtcaaac actttgccgc atacggcgcg gtagaaggcg gtaaagagta caacaccgtc
    43381 gatatgagtc cgcagcgcct gtttaatgat tatatgccgc cgtacaaagc ggggctggac
    43441 gcaggcagcg gcgcggtgat ggtggcgctg aactcgctga acggcacgcc agccacctcc
    43501 gattcctggc tgctgaaaga tgttctgcgc gaccagtggg gctttaaagg catcaccgtt
    43561 tccgatcacg gtgcaatcaa agagctgatt aaacatggca cggcggcaga cccggaagat
    43621 gcggtgcgcg tggcgctgaa atccggaatc aacatgagta tgagcgacga gtactactcg
    43681 aagtatctgc ctgggttgat taaatccggc aaagtgacca tggcagagct ggacgatgct
    43741 gcccgccatg tactgaacgt taaatatgat atggggttgt ttaacgaccc atacagccat
    43801 ttggggccga aagagtctga cccggtggat accaatgccg aaagccgcct gcaccgtaaa
    43861 gaagcgcgtg aagtggcgcg cgaaagcctg gtgttgctga aaaaccgtct cgaaacgtta
    43921 ccgctgaaaa aatcggccac cattgcggtg gttgggccac tggcggacag taaacgtgac
    43981 gtgatgggca gctggtccgc agccggtgtt gccgatcaat ccgtgaccgt actgaccggg
    44041 attaaaaatt ccgtcggtga aaacggtaaa gtgctgtatg ccaaaggggc gaacgttacc
    44101 agtgacaaag gcattatcga tttcctgaat cagtatgaag aagcggtcaa agtcgatccg
    44161 cgttcgccgc aagagatgat tgatgaagcg gtgcagacgg cgaaacaatc tgatgtggtg
    44221 gtggctgtag tcggtgaagc acaggggatg gcgcacgaag cctccagccg gaccgatatc
    44281 actattccgc aaggccaacg tgacttgatt gcggcgctga aagccaccgg taaaccgctg
    44341 gtgctggtgc tgatgaacgg gcgtccgctg gcgctggtga aagaagatca gcaggctgat
    44401 gcgattctgg aaacctggtt tgcggggact gaaggcggta atgcaattgc cgatgtattg
    44461 tttggcgatt acaacccgtc cggcaagctg ccaatttcct tcccgcgttc tgtcgggcag
    44521 atcccggtgt actacagcca tctgaacacc ggtcgtccgt ataatgccga caagccgaac
    44581 aaatacactt cgcgttattt tgatgaagct aacggagcgc tttatccgtt cggctatggt
    44641 ctgagctata ccactttcac cgtctctgat gtgaaacttt ctgcgccgac catgaagcgt
    44701 gacggcaaag tgacggccag cgtgcaggtg acgaacaccg gtaagcgcga gggggccacg
    44761 gtagtgcaga tgtacttgca ggatgtgacg gcttccatga gtcgccctgt gaaacagctg
    44821 aaaggctttg agaaaatcac cctgaaaccg ggcgaaactc agactgtcag cttcccgatc
    44881 gatattgagg cgctgaagtt ctggaatcaa cagatgaaat atgacgccga gcctggcaag
    44941 ttcaatgtat ttatcggcac tgattccgca cgcgttaaga aaggcgagtt tgagttgctg
    45001 taatgcttta gtaagggcgc gacgtttgcg ccctttgcct gatgttcata tcttaacttg
    45061 ctgaattttc gccctttgta ggccggataa ggcgctcgcg ccgcatccgg caggaaacgc
    45121 cggaccgaat atgcctctta agtatttatc tgattctgca actgaatcct tccgctcagg
    45181 ctaactctgt cattatcaac tatgcttttc tcttaattcg ctgaaaacaa cagtaaaaga
    45241 atgaggaaag catcatgcca ctcttaaagc tctgggcagg ttcactggtt atgttggcag
    45301 ccgtgagcct gccgctacag gcggcttccc ccgttaaagt cggttcaaaa atcgataccg
    45361 aaggcgcgct gctcggcaac atcatctttc aggtactcga aagccacggc gtaccgacgg
    45421 tgaataaagt gcaactcgga acgactcctg tggggcgcgg ggcgattact tccggtgaac
    45481 tggatatcta tccggaatat accggcaatg gcgctttctt ctttaaagat gaaaacgatg
    45541 cagcgtggaa aaatgccggg cagggctacg agaaagtcaa aaagctcgat gcggagcaca
    45601 acaagttaat ctggctgacg cccgcgcctg caaataacac ctggactatc gccgtgcgtc
    45661 aggatgtggc agagaaaaac aaactcactt cgcttgccga cctgagtcgt tatctgcaag
    45721 agggcggcac cttcaaactg gcagcctcgg cagagtttat cgaacgcgcc gatgcgttac
    45781 ccacgtttga aaaagcctat ggctttaagc tcggtcagga tcagttgctg tcactggctg
    45841 gtggcgacac ggcggtgacg attaaagccg ctgcccagca aacctctggc gttaatgccg
    45901 caatggctta cggcactgac ggtccggtcg cggcgctggg gctgcaaacc ttaagcgatc
    45961 cgcaaggcgt gcaacctatc tacgcgcctg caccagtggt gcgtgaatcg gtgttgaagg
    46021 agtatccgca aatggcacag tggctacagc cagtcttcgc cagcctcgat gcaaaaacat
    46081 tgcagcaact gaatgccagc attgcagtgg aaggactgga tgccaaaaaa gtggctgccg
    46141 actacctgaa acaaaaaggg tgggcgaagt aatttcccgt gacttatcta cgtattaatc
    46201 ctgttctggc gctgctgttg ttgctgacgg caatcgcagc ggcgctgccg tttatcagtt
    46261 acgcgcctaa tcgtttagtc tcgggtgaag ggcttcatct ctggcaactg tggccgcaaa
    46321 cgatctggat gctggtgggc gttggttgcg cctggctgac agcctgtttt gtccccgcta
    46381 aaaaaggcag catttttgca ctcattctgg cgcaattcgt cttcgtattg ttggtgtggg
    46441 gagctggaaa ggcggcgacc caactggcgc aaaatggcag tgcgctggcg cgtaccagcc
    46501 tcggcagagg tttctggctg gctgcggcgc tggcattgct ggcctgtagc gatgccatcc
    46561 gccgaatctc cacgcatccg ctgtggcgct ggttgttgca tatgcagatt gccattattc
    46621 cgctgtggtt gctgtactcc ggcacgctta acgatctctc actaatgaaa gaatacgcca
    46681 accgtcagga tgtgtttgac gacgcgctgg cacaacatct gacgttgctg tttggtgcgg
    46741 tgctgcctgc gttagtgatt ggtgtgccgt tgggcatctg gtgctacttt tccactgtgc
    46801 ggcagggggc aattttttct ctgctcaatg tcattcagac cgtgccttcg gtggcgctct
    46861 ttggcctgtt gattgcgccg cttgccgcgc tggttacggc ctttccgtgg ctggggaagc
    46921 tcggcatagc aggaaccgga atgacacccg cactgattgc gctggtgctc tatgccttgc
    46981 tgccgctggt gcgaggcgtg gtagtcggtt tgaaccagat cccgcgcgat gtgctggaga
    47041 gcgccagagc gatggggatg agcggggcgc agcgtttcct gcatgttcag ttaccgctgg
    47101 cgttactggt atttctgcgc agcctgcggg tggtgatggt gcaaactgta ggtatggcgg
    47161 tgattgcggc gttaatcggc gcaggcggtt ttggtgcgct ggttttccag gggctgctaa
    47221 gcagcgccat tgatttagtg ttgctggggg tgatcccggt aattgttctg gcggtgctta
    47281 ccgacgcgct gttcgatttg cttatcgcac tgctgaaggt gaaacgtaat gattgaattt
    47341 agccatgtca gcaaactgtt cggcgcacaa aaagccgtta acgatctcaa tctcaatttt
    47401 caggaaggga gtttttcggt gctgattggc acatctggct ccggcaaatc caccaccctg
    47461 aaaatgatta accgcctggt ggagcatgac agcggagtga tccgctttgc cggagaagaa
    47521 attcgctcgc tgccagtact ggagttgcgc cgccggatgg gctatgccat tcaatctatt
    47581 ggcctgttcc cccactggag cgtggcgcaa aacatcgcta ccgtgccgca attacaaaaa
    47641 tggtcgcggg cgcggattga cgatcgtatc gacgaattaa tggcgctact ggggctggag
    47701 tcaaatttgc gtgagcgtta tccgcatcag ctttccggtg gtcagcagca acgtgtggga
    47761 gtggcgcgtg cactggctgc cgatccgcaa gtcttactga tggatgaacc ttttggcgca
    47821 ctggacccgg taacgcgcgg cgcgttgcaa caagagatga cgcgcattca ccgtttgctg
    47881 gggcgcacta ttgtgctggt cactcatgat attgatgagg cgctacggct ggcagaacat
    47941 ctggtattga tggatcacgg tgaagtggtg cagcagggga atccgctgac gatgctgact
    48001 cgtccggcga atgattttgt ccgccagttt tttggacgta gtgaactggg ggtgcacctg
    48061 ctttcgttac gtagcgtggc ggattacgtg cgtcgcgaag aacgggcaga aggtgaggca
    48121 ctggcagaag agatgacgct acgcgatgcg ctctccctgt ttgtcgcgcg gggatgcgag
    48181 gtgctgccgg tggtgaacac gcagggccag ccttgcggca cgctgcattt tcaggatctg
    48241 ctggtggagg cgtaagcgta tgaagatgtt gcgcgatccg ctgttctggc tcattgctct
    48301 ttttgtggcg ctgattttct ggctgcctta cagccagccg ctgtttgctg ccttgttccc
    48361 acaactgcca cgacccgttt atcagcaaga aagttttgca gctctggcac tggctcattt
    48421 ctggctggtg ggaatttcga gtttgtttgc ggtgatcatt ggcacaggtg ccggaattgc
    48481 tgttactcgc ccgtggggcg cggaatttcg cccactggtg gaaactattg ccgccgttgg
    48541 acagactttt ccgcctgtcg cagtgctggc gattgccgtt ccggtgatcg gctttggtct
    48601 gcaaccagcg attatcgcct tgatccttta cggtgtgctg cccatcctgc aggtgacact
    48661 tgccgggctg ggagcgattg atcccagcgt gacagaagtt gcgaaaggta tgggaatgag
    48721 tcgaggtcag cgactgcgta aggtcgagct accgctggcg gctccggtga ttctggcggg
    48781 cgtgcgaact tcggtgatta tcaacattgg tacggcgacg atcgcctcaa cggtaggggc
    48841 cagtacgctg ggtacgccca tcatcatcgg gcttagcgga tttaataccg cgtatgtgat
    48901 ccagggggcg ttactggtgg cactggcggc gatcatcgca gaccgcctgt ttgaaaggct
    48961 ggtgcaggcg cttagccagc acgcaaaata aaggtataac ctgcgagcat gacgccacca
    49021 attccgccta acgccataaa caggaacagg gcgatgaccc caattttagc tatgcgcata
    49081 atgcactcct tatgttaacg aaaggattgt acagtaaagc gcatttgtta acgaatcatt
    49141 aaataccgag tgggaaaata tcatggcctt gttcttgcca actggtgagt tgctgctgtt
    49201 gggcggaggt tcgattttca ccgcaccaca ccagcaatgt acggccttcg aatagttcag
    49261 ggcgtagttg attgagcgag tgggcgagcg aggacatcaa tgcgccatcc ttgttgactg
    49321 gcaatccagc cctccagcca cagacgggtg gtatcctgaa tattccagcc aaccaccagc
    49381 gcatctttac cctgtttttt acgtgccgaa gccagacaaa tggcgatgta gttgatcagt
    49441 acgccgtcga ggatcgccag cagcgcctgg agagtcggtt gttggcactg aagccgtcgg
    49501 cgcagaggaa taaacagatg tgtggtgagt gtctgggcgg ggtaatcctg accgcgctct
    49561 ttgatccacg ttcgcaggct atgtagattg c
//
LOCUS       DAEEGA010000023        48607 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEEGA010000023 DAEEGA010000000
VERSION     DAEEGA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48607
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.023"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            30..1280
                     /locus_tag="KLH46_001365"
     CDS             30..1280
                     /locus_tag="KLH46_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBE0378106.1"
                     /translation="MSKLESLPEFELPEITDNELLVSVISDSVCLSTWKAALLGSEHK
                     RVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEY
                     FGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHR
                     MGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLA
                     ASKGIELVYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLN
                     FFAGLTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHI
                     GGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETH
                     GIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            1277..2242
                     /gene="yggF"
                     /locus_tag="KLH46_001366"
     CDS             1277..2242
                     /gene="yggF"
                     /locus_tag="KLH46_001366"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBE0378107.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPHTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2264..2773
                     /gene="fumE"
                     /locus_tag="KLH46_001367"
     CDS             2264..2773
                     /gene="fumE"
                     /locus_tag="KLH46_001367"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBE0378108.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2770..3483
                     /locus_tag="KLH46_001368"
     CDS             2770..3483
                     /locus_tag="KLH46_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBE0378109.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3455..4137)
                     /locus_tag="KLH46_001369"
                     /pseudo
     CDS             complement(3455..4137)
                     /locus_tag="KLH46_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
     gene            complement(4131..4808)
                     /locus_tag="KLH46_001370"
     CDS             complement(4131..4808)
                     /locus_tag="KLH46_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBE0378110.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4796..5179)
                     /locus_tag="KLH46_001371"
     CDS             complement(4796..5179)
                     /locus_tag="KLH46_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBE0378111.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5195..5503)
                     /locus_tag="KLH46_001372"
     CDS             complement(5195..5503)
                     /locus_tag="KLH46_001372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378112.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5504..6082)
                     /locus_tag="KLH46_001373"
     CDS             complement(5504..6082)
                     /locus_tag="KLH46_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBE0378113.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6105..6536)
                     /locus_tag="KLH46_001374"
                     /pseudo
     CDS             complement(6105..6536)
                     /locus_tag="KLH46_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6908..7927
                     /gene="epd"
                     /locus_tag="KLH46_001375"
     CDS             6908..7927
                     /gene="epd"
                     /locus_tag="KLH46_001375"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBE0378114.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7977..9140
                     /gene="pgk"
                     /locus_tag="KLH46_001376"
     CDS             7977..9140
                     /gene="pgk"
                     /locus_tag="KLH46_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBE0378115.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9355..10434
                     /gene="fbaA"
                     /locus_tag="KLH46_001377"
     CDS             9355..10434
                     /gene="fbaA"
                     /locus_tag="KLH46_001377"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBE0378116.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10625..11485
                     /gene="mscS"
                     /locus_tag="KLH46_001378"
     CDS             10625..11485
                     /gene="mscS"
                     /locus_tag="KLH46_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBE0378117.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11624..12259
                     /gene="argO"
                     /locus_tag="KLH46_001379"
     CDS             11624..12259
                     /gene="argO"
                     /locus_tag="KLH46_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBE0378118.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12352..13092
                     /locus_tag="KLH46_001380"
     CDS             12352..13092
                     /locus_tag="KLH46_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBE0378119.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13259..14209
                     /locus_tag="KLH46_001381"
     CDS             13259..14209
                     /locus_tag="KLH46_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0378120.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14151..15629)
                     /gene="scpC"
                     /locus_tag="KLH46_001382"
     CDS             complement(14151..15629)
                     /gene="scpC"
                     /locus_tag="KLH46_001382"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HBE0378121.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15653..16429)
                     /gene="scpB"
                     /locus_tag="KLH46_001383"
     CDS             complement(15653..16429)
                     /gene="scpB"
                     /locus_tag="KLH46_001383"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBE0378122.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHT
                     MNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16440..17435)
                     /gene="meaB"
                     /locus_tag="KLH46_001384"
     CDS             complement(16440..17435)
                     /gene="meaB"
                     /locus_tag="KLH46_001384"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBE0378123.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYHQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17428..19572)
                     /gene="scpA"
                     /locus_tag="KLH46_001385"
     CDS             complement(17428..19572)
                     /gene="scpA"
                     /locus_tag="KLH46_001385"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBE0378124.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTIADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19776..20669)
                     /gene="argP"
                     /locus_tag="KLH46_001386"
     CDS             complement(19776..20669)
                     /gene="argP"
                     /locus_tag="KLH46_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBE0378125.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21052..21711
                     /gene="rpiA"
                     /locus_tag="KLH46_001387"
     CDS             21052..21711
                     /gene="rpiA"
                     /locus_tag="KLH46_001387"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBE0378126.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21967..23199
                     /gene="serA"
                     /locus_tag="KLH46_001388"
     CDS             21967..23199
                     /gene="serA"
                     /locus_tag="KLH46_001388"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBE0378127.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23428..23487
                     /gene="ibsC"
                     /locus_tag="KLH46_001389"
     CDS             23428..23487
                     /gene="ibsC"
                     /locus_tag="KLH46_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBE0378128.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23820..23879
                     /locus_tag="KLH46_001390"
     CDS             23820..23879
                     /locus_tag="KLH46_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBE0378129.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23980..24582)
                     /locus_tag="KLH46_001391"
     CDS             complement(23980..24582)
                     /locus_tag="KLH46_001391"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBE0378130.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24603..24786)
                     /gene="ssrS"
                     /locus_tag="KLH46_001392"
     ncRNA           complement(24603..24786)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KLH46_001392"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24828..25157)
                     /gene="zapA"
                     /locus_tag="KLH46_001393"
     CDS             complement(24828..25157)
                     /gene="zapA"
                     /locus_tag="KLH46_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBE0378131.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25325..25903
                     /gene="ygfB"
                     /locus_tag="KLH46_001394"
     CDS             25325..25903
                     /gene="ygfB"
                     /locus_tag="KLH46_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBE0378132.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25929..27254
                     /gene="pepP"
                     /locus_tag="KLH46_001395"
     CDS             25929..27254
                     /gene="pepP"
                     /locus_tag="KLH46_001395"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBE0378133.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27251..28429
                     /gene="ubiH"
                     /locus_tag="KLH46_001396"
                     /gene_synonym="visB"
     CDS             27251..28429
                     /gene="ubiH"
                     /locus_tag="KLH46_001396"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBE0378134.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28452..29654
                     /gene="ubiI"
                     /locus_tag="KLH46_001397"
                     /gene_synonym="visC"
     CDS             28452..29654
                     /gene="ubiI"
                     /locus_tag="KLH46_001397"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBE0378135.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29728..29811)
                     /locus_tag="KLH46_001398"
     CDS             complement(29728..29811)
                     /locus_tag="KLH46_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378136.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30102..31196
                     /gene="gcvT"
                     /locus_tag="KLH46_001399"
     CDS             30102..31196
                     /gene="gcvT"
                     /locus_tag="KLH46_001399"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBE0378137.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKRGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31220..31609
                     /gene="gcvH"
                     /locus_tag="KLH46_001400"
     CDS             31220..31609
                     /gene="gcvH"
                     /locus_tag="KLH46_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBE0378138.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31728..34601
                     /gene="gcvP"
                     /locus_tag="KLH46_001401"
     CDS             31728..34601
                     /gene="gcvP"
                     /locus_tag="KLH46_001401"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBE0378139.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34868..35610
                     /locus_tag="KLH46_001402"
                     /pseudo
     CDS             34868..35610
                     /locus_tag="KLH46_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(35667..37100)
                     /gene="bglA"
                     /locus_tag="KLH46_001403"
     CDS             complement(35667..37100)
                     /gene="bglA"
                     /locus_tag="KLH46_001403"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBE0378140.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37145..37455
                     /locus_tag="KLH46_001404"
                     /pseudo
     CDS             37145..37455
                     /locus_tag="KLH46_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37619..38278
                     /locus_tag="KLH46_001405"
     CDS             37619..38278
                     /locus_tag="KLH46_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBE0378141.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38474..39454)
                     /gene="ygfZ"
                     /locus_tag="KLH46_001406"
     CDS             complement(38474..39454)
                     /gene="ygfZ"
                     /locus_tag="KLH46_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBE0378142.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSVSRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39697..39963
                     /gene="sdhE"
                     /locus_tag="KLH46_001407"
     CDS             39697..39963
                     /gene="sdhE"
                     /locus_tag="KLH46_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBE0378143.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39944..40351
                     /locus_tag="KLH46_001408"
     CDS             39944..40351
                     /locus_tag="KLH46_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HBE0378144.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40391..40912)
                     /gene="fldB"
                     /locus_tag="KLH46_001409"
     CDS             complement(40391..40912)
                     /gene="fldB"
                     /locus_tag="KLH46_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBE0378145.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            41024..41920
                     /gene="xerD"
                     /locus_tag="KLH46_001410"
     CDS             41024..41920
                     /gene="xerD"
                     /locus_tag="KLH46_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBE0378146.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41945..42655
                     /gene="dsbC"
                     /locus_tag="KLH46_001411"
     CDS             41945..42655
                     /gene="dsbC"
                     /locus_tag="KLH46_001411"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBE0378147.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42661..44394
                     /gene="recJ"
                     /locus_tag="KLH46_001412"
     CDS             42661..44394
                     /gene="recJ"
                     /locus_tag="KLH46_001412"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBE0378148.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44485..45583
                     /gene="prfB"
                     /locus_tag="KLH46_001413"
     CDS             join(44485..44559,44561..45583)
                     /gene="prfB"
                     /locus_tag="KLH46_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBE0378149.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45593..47110
                     /gene="lysS"
                     /locus_tag="KLH46_001414"
     CDS             45593..47110
                     /gene="lysS"
                     /locus_tag="KLH46_001414"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBE0378150.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47153..47701)
                     /gene="idi"
                     /locus_tag="KLH46_001415"
     CDS             complement(47153..47701)
                     /gene="idi"
                     /locus_tag="KLH46_001415"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBE0378151.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47756..47827
                     /locus_tag="KLH46_001416"
     CDS             47756..47827
                     /locus_tag="KLH46_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378152.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47824..47949)
                     /locus_tag="KLH46_001417"
     CDS             complement(47824..47949)
                     /locus_tag="KLH46_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378153.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47951..>48607)
                     /gene="uacT"
                     /locus_tag="KLH46_001418"
     CDS             complement(47951..>48607)
                     /gene="uacT"
                     /locus_tag="KLH46_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBE0378154.1"
                     /translation="IIVFIESMGMFLALGEIVGRKLSSHDIICGLRVDGVGTMIGGTF
                     NSFPHTSFSQNVGLVSVMRVHSRWVCISSGIILILFGMVPKMAVLVASIPQFVLGGAG
                     LVMFGMVLATGIRILSRCNYTTNRYNLYIVAISLGVGMTPTLSHDFFSKLPAVLQPLL
                     HSGIMLATLSAVVLNVFFNGYQHHADLVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE
                     "
BASE COUNT        11665 a        12188 c        13120 g        11634 t
ORIGIN      
        1 catcgggcat tatctgaaca taaaacacta tcagtaagtt ggagtcatta cccgaatttg
       61 aactgccaga aattaccgat aatgaactgt tagtgagtgt aatttctgac agcgtctgtt
      121 tatcgacctg gaaagcggcg ttactcggta gtgaacataa acgcgtaccc gacgatttag
      181 aaaatcatcc ggtcattacc gggcatgaat gtgccggggt tattgtcgaa gtgggtaaaa
      241 atctcactgg caaatataaa aaaggccagc gttttgtatt gcaaccggcg atggggttac
      301 caagcggata ttcagcgggc tacagctacg aatattttgg cggcaatgcc acttatatga
      361 ttattcccga aatcgccatt aatttgggct gcgtattacc gtatcacggc tcttattttg
      421 ctgcggcgtc gctggcagag cctatgtgct gcattattgg tgcttatcat gccaattatc
      481 acaccacgca atatgtttat gagcatcgca tgggcgtcaa acctggcggc aatattgcac
      541 tgctggcgtg tgcgggtccg atgggcattg gcgctatcga ttacgccatt aacggtggca
      601 tacaaccgtc acgggtggtg gtggtcgata tcgacgacaa acgcctggcg caggtgcaga
      661 agctgctgcc ggtggatctg gcggccagta aaggcattga gctagtgtat gtgaatacca
      721 aagggatgag cgatcctatc cagatgctgc gggcgctgac aggagatgtc gggttcgatg
      781 acatttttgt ttatgcggcg gtgcctgctg tcgttgagat ggctgacgaa ttactggcgg
      841 aagatggctg tctgaacttc tttgccgggc tgacggataa aaacttcaaa gtgccgttta
      901 atttctacaa cgtccattac aacagcacgc acgtagtcgg tacatccggc ggttcaacgg
      961 acgacatgaa agaggcgatt gcccttagcg ccacaggaca gttacagccg tcgtttatgg
     1021 tgacccatat tggtggcctg gatgcggtgc cagaaaccgt gctcaatctg ccggatatcc
     1081 ctggcggtaa aaaactcatt tataacggcg tgaccatgcc gctcactgcc attgccgatt
     1141 ttgccgaaaa aggcaaaacc gatccgctgt ttaaagagtt ggcgcggctg gttgaggaaa
     1201 cgcacggcat ctggaatgaa caggccgaga aatatctgct ggcacaattt ggcgttgata
     1261 tcggggaggc cgcgcaatga tgtccctggc gtggccatta tttcgcgtta cggaacaggc
     1321 agcgttggct gcctggccgc acaccggatg tggcgacaaa aataaaattg atggcctggc
     1381 ggtcaccgcg atgcgccagg cattaaacga cgtcgctttt cgtggacgag tggttatcgg
     1441 cgaaggagaa attgaccatg cgccgatgct gtggattggc gaagaggtgg gcaaaggcga
     1501 tgggccagaa gtcgatatcg cggttgatcc cattgaaggc acgcggatgg tggcgatggg
     1561 gcagagcaat gcgctggcgg tcatggcttt cgctcctcga gatagcctgt tacatgcgcc
     1621 cgatatgtat atgaaaaagc tggttgttaa tcgactggct gccggggcga ttgatctgtc
     1681 tttgcctctg gcggacaact tgcgtaacgt ggcgcgggcg ttaggtaaac cgctggacaa
     1741 gctgcgcatg gttacgctgg ataaaccgcg tctgagtgcc gcgattgaag aagcaacaca
     1801 gctgggcgtg aaggtttttg ccctgccgga tggcgatgtc gccgccagcg tgctgacctg
     1861 ctggcaggat aatccttacg atgtgatgta caccatcggc ggtgcgccag aaggcgtgat
     1921 ttctgcctgt gccgttaaag cgttaggcgg ggatatgcag gccgagctga ttgacttttg
     1981 tcaggctaaa ggggattaca cggaaaatcg gcagattgca gagcaggagc gcaagcgttg
     2041 taaggcaatg ggcgttgacg ttaaccgtgt gtactcgctc gatgaactgg tgagggggaa
     2101 cgatatcctc tttagcgcta cgggcgtgac gggcggcgag ttggtgaacg gtatccaaca
     2161 gacggcgaat ggggtgcgga cgcagacatt actgatcggc ggcgcggacc aaacgtgtaa
     2221 tataatagac tccctgcatt gatggtgatc tatcggaaga ccaatggcga cgctgacaga
     2281 agatgatgtg cttgagcaac tggatgcaca ggacaattta ttttcattta tgaaaactgc
     2341 gcattctatt ttgctccagg ggatacgcca gtttctgccg tcgctgtttg tcgataacga
     2401 tgaagagatc gtcgaatatg cagtgaagcc gttactcgcc caaagcggcc cgcttgacga
     2461 tattgatgtt gcgctgcgtt tgatttatgc gctggggaaa atggataaat ggctgtatgc
     2521 cgatatcacg cacttttccc agtactggca ttacctgaac gaacaggatg aaacgcccgg
     2581 atttgccgat gatataacct gggattttat cagcaatgtc aacagtataa ctcgcaatgc
     2641 gacgctctac gatgcgttaa aagcgatgaa gttcgccgat ttcgccgtct ggtcagaggc
     2701 gcgttttagc ggaatggtca aaacggcgct gacgctggca gtaacgacca ccttaaagga
     2761 attaacgccg tgaaaattga attaacggtg aatgggctga acgtgcaagc gcagtaccat
     2821 gatgaagaga tagaacgcgt gcataaactg ttgctgcgta tgctggcggc cttacagacg
     2881 gtaaatccgc agcggcgtac ggtggttttt ctttgcgctc cgccggggac gggtaaatct
     2941 accctaacca ccttctggga atatctcgcg caacaagacc cggagctgcc cgctattcag
     3001 gcgctcccga tggatggttt tcaccattac aatagctggc tggacgcgca tcaattgcgc
     3061 cccttcaaag gcgcaccaga gacatttgac gttgcgaaac tggcggaaaa tctgcgccag
     3121 gtggtggaag gggattgtac gtggccgcag tacgatcgac aaaagcatga tcctgttgaa
     3181 gatgcgttgc acgttaccgc accactcgtc atcgtcgaag gaaactggtt gttgctggac
     3241 gacgagaagt ggtgccagct tgcgcaattt tgtgatttct cgatctttat caacgcgcca
     3301 gctgctgcgt tgcgggagcg tctggtgggg cgcaagctgg cgggaggggt atcgctggca
     3361 gatgcagaag ccttttatga ccgtaccgac gggccgaatg ttcgccgggt gctggaagag
     3421 agtctgcctg caaatttgac cttaatgatg accgctacgg gtgaatatcg tctggtggat
     3481 taacgttcct gattacacca tcttccagcc gcaccacacg agagaaatga cgccgggtaa
     3541 atgcggcgtc atgactgatt gctaccacgc tcgtgcctcg ctggcggcac ttctccagcc
     3601 agctttcaaa cacgctcaac caattttcgt caaaatcccg actcggctcg tccagcagta
     3661 atagtggcgg agagagtgct tccaggcagg cgacggcgac catccgtcgc tgcgcactat
     3721 gcagatccaa cgggtgcgag ttggcagtgt ctgctaactg acaacattgc agggcagcat
     3781 gggtacgttg agtgatttca tccgccgggc atttttgcag tttcaaacca aatgcgatct
     3841 cgtcagcgac ggtgctgtga aaaagttggt tttccgcttc ctgaaacaga acagtacgcc
     3901 aacctttgcc gcgcgctggc ggtttttcag gtttttcatc gcctgttgtt gcagcataac
     3961 cgtaccggct gtgggcgtaa gaagacctgc cattactcgt aacagcgtcg atttccctgc
     4021 gccattgtca ccggtcagcg ccaaccattc accttgtttg agttgcagag aaatgtcgca
     4081 aagacaatcc gttgccgcgc caggccagcg ataagatatc ttatttaacg ttaacatagt
     4141 ggctggagcg ctccgttttg caattgccac gcttgctggc agtagttcag gaaaggtgtg
     4201 cgatgacgtt caaaaagaat aatcagcgaa ccacgttcaa acgcccagtg ttgtaagcgc
     4261 tgcaacagca tttcgctggc ctgcggcgtc aggcggctaa aggcttcatc aagaattaag
     4321 agctttggct gcatcgcgat ggcgcaggcg atgatcacgc gctgtgtctc tccaccggag
     4381 agtgttgccg ggtgacgatg gcgcaacggc tggcactcgg tcagagtcag ggcggcatca
     4441 atccgcgcca taatctctgc ttcagcaaga cacagatttt ctggcccaaa ggcaacttct
     4501 tcttccacgc taaaggtaca gccggagagc tgcaaatagg gcgattgctg gacgagctgg
     4561 atggtggcgg attgttcatt aagcgggaga tgcccaatgg gcgtaccgag gagcgtcccc
     4621 gtgcctgtta tctctcttgg cagaaagtcc ggataccagc ccgccatcag ctgtgccagc
     4681 gtacttttgc cactgccgtt gtcaccaaag atggcgacca tccccggttt gacatagtga
     4741 aagtcataac agaacggagg atgcgttgag tgtgtcgggc aataacggaa ctgttctaac
     4801 gtaaccatat ccacgctccg aattctgtca gcatcagcaa gatcatggtg taacgcgcta
     4861 cgcgttgtag ggtgctatcg gctggtgccc acaacgttgt tcgtcggttg tggatccgaa
     4921 atgcccgcat atccagcgcc gcaccacgga cagccagatc gttaagcgca ttgtgtgtta
     4981 gcggaataat taacgcgggc atagcacgta aacgctgata ccagccctca tccagcggca
     5041 cgccgcgggc acgctgcgct tcatgaataa tcgctaattg ccgttttaac tgctcaacga
     5101 ccaataacgg cccggcaaac agataagcga cgcctggcgg caggcgagaa gcaaacagag
     5161 cgcgaataaa acattgcacg gggacgtact gcattcacaa ctgtgaggtt gaaacaatcg
     5221 ccagaatcag caaccagagc gtaatagcat gcgaccaccg ttctggcgag cgtggcgtgc
     5281 cgcttagcca ttctggtagc cagccaccgt gtaccagcca cagcccggct cccagcgaga
     5341 acatcagcca gacgacatat tttgcccgcc gccgcgtggc ctttagcgca ataaggcaga
     5401 aaaaggtcgc cgcgctatag atcggcaaaa tggtctgcgc gggcaggata agcgtggtgc
     5461 aggccgccaa tgcccacagt gttaatgagg taaatggatg catttagcgc acggcagcca
     5521 ttgccggaaa atggcgcgtg gtgcgaatcg gcagctggcg cagtaaaagc cagacaatca
     5581 ccgctgtaag aattttatcg accaggttcg cgcctatcac cgtaatcgcc accgactcca
     5641 ccaggttttg ccccatcgag tgcatccagg caacgaacaa atcggctccg ctaccggtga
     5701 caccgccgaa cagcgcagta cgcagcggta cagcgacgac cgttaccgca agcgtaataa
     5761 tcacgccgct gacaacgact tttggcaggg tgcgaaacca acccgctcgc gccagccaac
     5821 cggagaccag gccgatgacc atcgcgacgg gtgcaaatgc ggcggcgatc gggtcggtca
     5881 gcaatcccca gagtaaattg gtcagtaagc cggtcagcat tccgatcacc ggaccaagca
     5941 gtaccgcgct aataagtgtg ccgatggagt cgagaaatat aggtaatttg accatgctgg
     6001 ccagttgccc gccgatcata ttgatggcga tagatataac gatcaacacc agtgcctgac
     6061 tggaaaagtg gctacgcgcc ataagaaaac ctctttgaaa gtagttattt aacgggggaa
     6121 atatgcaact cgtcgtaacc agaaagcgcg caaggttggc ggctaaacgc cagttcctca
     6181 aggctaccga tcaccagttc aagcgtggtc cgcacggtac agccgggcat catatgaccg
     6241 ccaagcatcg tgccgtgtgg atcggaaacg cagagatgaa gatgctcacc actctgttcc
     6301 agcgtgccgt ttagtgagat cacttcgaat gtaccgttca ggagcgtggt gccttcttgc
     6361 ccggcatagc gcaaggcgac gtcagtcagg ctaccggtac agcctgcaat ctaggcggca
     6421 tgaagttgat gttgctgtgc aaaagcgtgt agctgggaaa aaacttcctg cccggggagg
     6481 aggcgcagtg cgtaaaagcg tgcggaggaa gaatatgagt agggcaatgt catcattgga
     6541 gtactccgaa actcggacgt attgcggata atatcgacgc tgcatcagtc atattctgat
     6601 ataagtcagg gtttgtgacg gcacaacgaa aagattttgc tacgcttatt cgtgattctg
     6661 caaagtggaa taaattccac aggggcgggg ctgaaaacgt gacgtaagtc agataattgc
     6721 tttctttgac tggacaatca ttccttttat tccacgtttc gcttatccta gctgaagcgt
     6781 ttcagtcgat taaatgttcg acaattaacc aatcagtcgc agtttgcgac aggtaaggtt
     6841 tccccggacg atttgctgga ttactctgtc tggcaaattt gctcagggaa aaccttgcag
     6901 gagatctatg accgtacgcg tagcgataaa tggcttcggt cgcatcgggc gtaatgtggt
     6961 tcgtgctttg tatgaatccg gacgccgggc ggaaattacc gtggtggcaa tcaacgaact
     7021 ggcggatgct gcgggcatgg cgcatttgtt gaaatatgac accagccatg gccgttttgc
     7081 atgggaagta cgacaggaac gcgatcaact ttttgttggt gatgacgcca tccgcgtatt
     7141 gcatgaacgt tcactgcaat cgctcccctg gcgtgaactt ggcgttgatg tagtcctcga
     7201 ctgcaccggc gtatatggct cccgcgagca tggcgaagcg catattgccg ccggggccaa
     7261 aaaagtgctc ttttcacatc ctggcagtaa cgatctcgac gcgaccgttg tttacggcgt
     7321 caatcaggat caacttcgtg cggaacaccg catcgtttct aacgcttcct gtaccacgaa
     7381 ttgcataatt cccgtcatca aattgttaga tgatgcgtac ggtattgagt ccggcactgt
     7441 gaccacaatt cactccgcca tgcacgatca acaggttatt gatgcatacc atcctgacct
     7501 gcgtcgcacc cgggcagcca gccagtcgat cattccggtc gatactaaac tggccgctgg
     7561 tatcacacga ttttttccgc aatttaacga tcgctttgaa gcgattgcgg tacgtgtgcc
     7621 aaccataaat gtgacggcaa tcgatttaag cgtgacggtg aagaaacctg taaaagccaa
     7681 tgaagtcaac ctgttgctgc aaaaagcagc acaaggtgca tttcatggta tagttgacta
     7741 tacggaattg ccgttggtct ctgtagattt taaccacgat ccgcacagtg ccattgtcga
     7801 tggcacccaa acccgggtca gtggcgcaca cctgatcaaa acgttggtct ggtgcgataa
     7861 cgaatggggc tttgctaacc gaatgctcga cacgacgtta gctatggcta ctgttgcttt
     7921 caggtaagac gcaagcagcg tctgcaaaac ttttagaatc aacgagagga ttcaccatgt
     7981 ctgtaattaa gatgaccgat ctggatcttg ctgggaaacg tgtatttatc cgtgcggatc
     8041 tgaacgtacc agtaaaagac gggaaagtaa ccagcgacgc gcgtatccgt gcttctctgc
     8101 cgaccatcga actggccctg aaacaaggcg caaaagtgat ggtaacttcc cacctgggtc
     8161 gtcctaccga aggcgagtac aacgaagaat tctctctgct gccggttgtt aactacctga
     8221 aagacaaact gtctaacccg gttcgtctgg ttaaagatta cctcgacggc gttgacgttg
     8281 ctgaaggtga actggttgtt ctggaaaacg ttcgcttcaa caaaggcgag aagaaagacg
     8341 acgaaaccct gtccaaaaaa tacgctgcac tgtgtgacgt gttcgtaatg gacgcattcg
     8401 gtactgctca ccgcgcgcag gcttctactc acggtatcgg taaattcgct gacgttgcgt
     8461 gcgcaggccc gctgctggca gctgaactgg acgcgctggg taaagcactg aaagaacctg
     8521 ctcgcccgat ggtggctatc gttggtggtt ctaaagtatc taccaaactg accgttctgg
     8581 actccctgtc taaaatcgct gaccagctga ttgttggtgg tggtatcgct aacaccttta
     8641 tcgcggcaca aggccacgat gtgggtaaat ccctgtacga agctgacctg gttgacgaag
     8701 ctaaacgtct gctgaccacc tgcaacatcc cggttccgtc tgatgttcgc gtagcaaccg
     8761 agttctctga aactgcaccg gctaccctga aatctgttaa cgatgtgaaa gctgacgagc
     8821 agatcctgga tatcggtgat gcttccgctc aggaactggc tgaaatcctg aagaatgcga
     8881 aaaccattct gtggaacggt ccggttggcg tgttcgaatt cccgaacttc cgcaaaggta
     8941 ctgaaatcgt ggctaacgct atcgcagaca gcgaagcgtt ctccatcgct ggcggcggcg
     9001 acactctggc agcaatcgac ctgttcggca ttgctgacaa aatctcctac atctccactg
     9061 gcggcggcgc attcctcgaa ttcgtggaag gtaaagtact gcctgcagta gcgatgctcg
     9121 aagagcgcgc taagaagtaa aaaatcacag ggcagggaaa cctgcacttg tttcagcgcg
     9181 cttttagagc acgcattttt tcatactcta aataattcga gttgcaggaa ggcgacaaga
     9241 gagtgaatcc ccaggagctt acataagtaa gtgactgggg ggagcgaacg cagacgcagc
     9301 acatgcaact tgaagtatta tgagtataag gcccgacgat acaggacaag agacatgtct
     9361 aagatttttg atttcgtaaa acctggcgta atcactggtg atgacgtaca gaaagttttc
     9421 caggtagcaa aagaaaacaa cttcgcactg ccagcagtaa actgcgtcgg tactgactcc
     9481 atcaacgccg tactggaaac tgctgctaaa gttaaagcgc cggttatcgt tcagttctcc
     9541 aacggtggtg cttcctttat cgctggtaaa ggcgtgaaat ctgacgttcc gcagggtgct
     9601 gctatcctgg gcgcgatctc tggtgcgcat cacgttcacc agatggctga acattatggt
     9661 gttccggtta tcctgcacac tgaccactgc gcgaagaaac tgctgccgtg gatcgacggt
     9721 ctgctggacg cgggtgaaaa acacttcgca gctaccggta agccgctgtt ctcttctcac
     9781 atgatcgacc tgtctgaaga atctctgcaa gagaacatcg aaatctgctc taaatacctg
     9841 gagcgtatgt ccaaaatcgg catgactctg gaaatcgaac tgggttgcac cggtggtgaa
     9901 gaagacggcg tggacaacag ccacatggac gcttctgcac tgtacaccca gccggaagac
     9961 gttgattacg catacaccga gctgagcaaa atcagcccgc gtttcaccat cgctgcgtcc
    10021 ttcggtaacg tacacggtgt ttacaagccg ggtaacgtgg ttctgactcc gaccatcctg
    10081 cgtgattctc aggaatatgt ttctaagaaa cacaacctgc cgcacaacag cctgaacttc
    10141 gtattccacg gtggttccgg ttctactgct caggaaatca aagactccgt aagctacggc
    10201 gtagtgaaaa tgaacatcga taccgatacc caatgggcaa cctgggaagg cgttctgaac
    10261 tactacaaag cgaacgaagc ttatctgcag ggtcagctgg gtaacccgaa aggcgaagat
    10321 cagccgaaca agaaatacta cgatccgcgc gtatggctgc gtgccggtca gacttcgatg
    10381 atcgctcgtc tggaaaaagc attccaggaa ctgaacgcga tcgacgttct gtaagtcatt
    10441 cctttctgct tatctcaagg cccgctctgc gggtcttttt ttcgccaaaa gcaaggtgat
    10501 tcagatgaga aatctgtgat ctatttggca aaattatgct ttattgttta cccttgtcag
    10561 actgcccgtc ataaggcggc ggagtgtatt tctccatttt gagtcagttg aaaaggaata
    10621 ttgaatggaa gatttgaatg ttgtcgatag cataaacggc gcgggaagct ggctggtagc
    10681 taaccaggcg ctgctgctaa gttatgcagt aaacatcgtg gcggcactcg cgatcatcat
    10741 cgttggtttg attatcgcgc ggatgatttc caacgcggtg aatcgcctga tgatctcccg
    10801 taaaatcgat gccactgttg ctgattttct ttctgcatta gtccgttacg gtattatcgc
    10861 ctttacgcta atcgctgccc tgggacgcgt gggtgtacaa acagcgtcag tcattgctgt
    10921 actcggtgcc gcaggcttag ctgttggtct ggctttgcag gggtcacttt ctaacctggc
    10981 cgctggcgtg ttacttgtca tgttccgccc gttccgtgcc ggagaatatg ttgacctcgg
    11041 cggcgtagcc ggtactgtgc tgagtgtgca gattttctcc accaccatgc gtactgcaga
    11101 cggtaaaatt atcgttattc cgaacggtaa aattattgcc ggaaatatta ttaacttctc
    11161 ccgcgagcca gttcgccgta acgaatttat tattggcgtg gcgtatgatt ccgatatcga
    11221 tcaggttaag cagatcctga ccaatattat ccagtctgaa gatcgcattt tgaaagatcg
    11281 cgaaatgact gtgcgcctga acgaacttgg tgcatcgtcg attaatttcg tggtccgcgt
    11341 ctggagcaac agcggcgatc tgcaaaacgt gtactgggat gtgctggagc gtattaaacg
    11401 tgaatttgat gccgccggta tcagcttccc gtacccgcaa atggatgtga actttaagcg
    11461 ggtgaaagaa gacaaagctg cgtaatcaac gctgaggcca gataatactc tctggcctct
    11521 ctcttattag ttattctgat tgccaattaa tattatcaat ttccgctaat aacaatcccg
    11581 cgatatagtc tctgcatcag atacttaatt cggaatatcc aacgtgtttt cttattactt
    11641 tcaaggtctt gctcttgggg cagctatgat tctgccgctc ggcccacaaa atgcttttgt
    11701 gatgaatcag ggcattcgtc gtcagtacca cattatgatt gccttacttt gcgctatcag
    11761 cgatttggtc ctgatttgcg cggggatttt tggtggtagc gcgttattga tgcagtcgcc
    11821 gtggttgcta gcgctggtca cctggggcgg cgtagtcttc ttgctctggt atggttttgg
    11881 cgcttttaaa acagcgatga gtagtaatat tgagttagct agtgccgaag tcctgaagca
    11941 aggcagatgg aaaattatcg ccaccatgtt ggcagtgacc tggctgaatc cgcatgttta
    12001 cctggatact tttgttgtac tgggcagcct tggcgggcag cttgacgtcg aaccaaaacg
    12061 ttggtttgcg ctcgggacaa ttagcgcctc tttcctgtgg ttctttggtc tggctattct
    12121 cgcagcctgg ctggcaccgc gcctgcgcac ggcaaaatca cagcgcatta tcaatctggt
    12181 agtgggatgt gtcatgtggt ttatagcctt gcagctggcg agagacggta ttgctcatgc
    12241 acaagccttg ttcagttagg cgctatctga tggaaaaata aaacagaggc gctaagcttg
    12301 cctccagagg tcctgaattt tgggcaagtc aattaacacg gagagactaa cgtgaagttc
    12361 aaagttatcg ccctggcggc attaatgggt attagcggga tggcagcgca ggctaacgaa
    12421 ttgccggatg gaccgcatat tgtcacctcc ggtacggcaa gtgtggatgc ggtgccagac
    12481 attgccactc ttgcgattga agttaacgtg gccgcgaagg atgccgctac agccaagaaa
    12541 caggcagatg agcgcgtcgc acaatacatt tccttccttg aactcaatca gatcgcgaaa
    12601 aaagatatca gctcagcgaa tttacgcacc cagccagatt atgactatca ggatggtaaa
    12661 agtatcctga aaggctaccg agctgtgaga acggtggaag tcacgctccg tcagttagac
    12721 aaactgaatt ccttgctgga tggcgcgctg aaggcgggtc ttaacgaaat tcgttctgtg
    12781 tcgctgggcg tggcgcagcc ggatgcctat aaagacaaag cgcgtaaggc agcgattgat
    12841 aacgcgattc atcaggcgca ggaactggcg aacggctttc atcgtaaact ggggccggta
    12901 tatagcgtgc gctaccatgt ttccaactat cagcccagcc caatggtgcg gatgatgaaa
    12961 gccgatgccg cgccggtgtc tgcccaggaa acttacgagc aggccgctat tcagtttgat
    13021 gatcaggtcg atgtggtctt ccagttagaa cctgtggatc aacaacccgc taaaacacct
    13081 gcagcacaat aatacttgta gcccgacgta ttcgtcgggc taatgccttt acccgatatt
    13141 gcctcctttt tctgtaattt tctgtaaaac catgacgtaa tcacctatct ttgctcgcgg
    13201 tttcatcaag gataggctga ctgaaacggt taagcggaca aggatgcggg gaggggggat
    13261 ggacattttt atttcgaaaa agatgcgcaa tttcatttta ttagcgcaaa ccaataatat
    13321 tgcccgggcg gcagaaaaaa tccatatgac ggcttcgcca tttggtaaaa gcattgccgc
    13381 gctggaagaa caaattggct atacgctatt tacccgcaaa gataataata tcagcctcaa
    13441 taaagccggt caggaactgt atcaaaaact gttcccggtt tatcaacgac tttctgcgat
    13501 tgataatgaa atccataact cagggcgtcg ttcacgagaa attgtgattg gcatagataa
    13561 tacttatccg acaattattt tcgatcagtt gattagtctc ggagataagt atgaaggcgt
    13621 gactgcccag ccagttgagt tcagcgaaaa cggcgttatc gataatcttt ttgaccgtca
    13681 gttagatttt attatctccc cacagcatgt atctgcacga gttcaggaac tggaaaacct
    13741 gactatcagt gaattaccac cgctgcgatt gggctttctc gtttctcgcc gttatgaaga
    13801 gcgtcaagag caagagctgc ttcaggagct gccctggcta caaatgcgct ttcaaaaccg
    13861 ggctaacttc gaagcaatga ttgatgcgaa tatgcgacct tgtgggatca atcccaccat
    13921 tatttatcgt ccctacagtt ttatggccaa aatcagcgcc gtggagcgcg gacattttct
    13981 gacggtgatc ccgcattttg cctggcgact ggtgaatccg gcaacgctga aatattttga
    14041 tgcgccgcac aggccgatgt atatgcagga atatctttat tcaatcagaa atcatcgtta
    14101 taccgccacg atcttcagca tattgctgaa gatcgtgacg ggacaaacca ttaacccagc
    14161 atcgagccgg ttgcaattaa attacggtgt aagtcgaaga cgtggctaag atcgtggtga
    14221 atatgtccgc cgggcgcatt ttccagatag cgatgcagat aatcccgata cataggatgt
    14281 gcacaattat caatgatagt gcgggcgcgt tgaagcgggg aaagaccgcg cagatcggcg
    14341 atcccttgtt cggtgatgat cactttgacg ctgtgttcgc tgtgatcaac atggctgcac
    14401 attggcacga cggttgagat cttgccttct ttagcaatcg acggagccat aaagatcgac
    14461 agatacgcgt tgcgttcaaa atcaccgctg ccgccgatgc cgttcatcag atcgaccccg
    14521 gctacgtgcg ttgaatttgc atgcccgtaa atatcaaact ccaggccgac gttcagagcg
    14581 atgacgccca gacgacggat gatttccggg ttattggaaa tctcctgcgg acgcaacaca
    14641 atgcggctgg caaagtaatc catattgtcg taaatcttgc gcagggaatc ggccgagatt
    14701 gtcaggctgg aggcgctggc cccgctgatt ttgccggttt ccagtaaatg caccaccgat
    14761 tcctgtagca cttccgaata catcatgaac ggaggaattt ccgggttttc ccccagacgt
    14821 gccattaccg cattattgat attgcccacg ccactttgca gcggcagaaa ttccggcgga
    14881 atacgcccat gcgccatttc ctgcaataag aacgtgacca cgttatcggc aatctgctgg
    14941 cacatgggat tttgcttatc cagcatatta ccggcgtcgg gcaagttggt ttccacgacg
    15001 gcgacaatct ttttcggatc gatttgcaca tagcgggtac cgacgcgatc cattgcatgg
    15061 aagatcgaca cgctattgcg ccgcggtggc gcgccaggaa tcacaatatc tgccagttct
    15121 gcaacgcgcg gatcgtgata gtgattaagt tcaatgatca ctttcttcgc tcgcagcagc
    15181 caggtcggcg cattaccgat cccgctggtt aaccagactc gaccatccgg tgccagtgcc
    15241 gatgcttcaa tgacggcaac atcaatgtcg ccgaagaaac cgtaattgac catttgcgcc
    15301 acttcgctca aatgcaggtc aacgaaactc accgcgccct gattgatctt tttacgtaaa
    15361 ctggacgatg tttgatatgg cgcacgccag gaaacagcat cggcgtcaga aagtacatcg
    15421 tcagcggcgg cgctgattga cgcccccgtc agcaggcgaa tttgatacgg ctttttggcc
    15481 tcatgctgtt cgttagctct gcgggcaatc gcggcgggta gggctttcgg cgaacccgct
    15541 ggggtaaagc cgctaaatgc caccatgtcg ttatgctgga taatttctgc cgcttcatcg
    15601 gcggtcatcc ttgtccactg agtttccatt tttactcctt cgttcgcagg gattaatgac
    15661 caacgaaatt aggtttacgt ttttcgagga aagcgttcat cccttcctgg taatcttcgc
    15721 tgtcataaac cgcgcggcgc atcccctgaa tacgttcaaa ttcatcggag ttcatggtgt
    15781 gtgcttcgcc cagtacacgc agctcttctt tgataacggc aatggctaac ggcgctttct
    15841 cagagatgtg gtgcgccatt tgtaaggtga aatcttccac ttccacaaca tggttgagga
    15901 tgccgacagc cagcgcgcgc tgggcggtga ttggcgaagc ggtaaaaatc agctctttga
    15961 caatgtggaa gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta tacgggacgc
    16021 cgaggtttac aggcgtcatt gagaaggttg aggtactggc ggcgatgatc agatcggaac
    16081 tcatgatcat ttcaaatgcg ccaccccaaa cactaccttc caccatcgaa atgatcggtt
    16141 tcgggaattt ttggatcatg cgggtgattt gacgcaatgg atcatcatag gagagcggat
    16201 cgcgaccgcc agacggcagt tcgtgaatat cgtgacctgc ggagaagact ttggatccac
    16261 ttggcgcccg caaaatgata cagcgaattt ccggccggtt gagatcgctt aacgcctgca
    16321 taagatcatc aataaagact ttacttaagg cattaagttt tcgtccatag ttaaactcaa
    16381 tgaccgccac tttgttgata gtggcaacgt tcacatactg ataagacata aaaattcctt
    16441 taatcaaaat attgcgtctg gataaattca ctgagctgcc gcaggccggt gcgcggtgag
    16501 agcgtattgt ttttgaccgc taaaagcgtc tggtggtaat agcgatcgaa atcttcattc
    16561 gcgaacaggt gattcagtac ttcttcttcg gtctgcttac gcagccattc caccgattgt
    16621 tgttgccgca cttgttgtaa acgaccactg gcagttagcg cggttttgaa gtcgatgatg
    16681 gcgtgccaga tctcatcgat tccacgtttt tccagtgcgc tacaagtcag accccgtggc
    16741 tgccattcgt cgtatttacg tcgcagaata tgcagggcac tctcgtacat atgccgggca
    16801 atggcgacat tggtatggtt atcgccatcg tctttgttga taacgagcag atcagccact
    16861 tccatcagcc cttttttaat gccctgcaga tcatcgccgc caccggcaat ttgcaacgag
    16921 ataaaacagt ccaccatgcg ggcgacttcc gtttccgact gcgcgacgcc aaccgtttcg
    16981 acaatcacta cgtcataacc cgccgcttcg cataacagca ttaattcccg tgctcgctga
    17041 ctggcaccgc ccagatgacc ggaggagggt accgggcgaa taaacgccgc ctcggcacgc
    17101 gccaggtcat tcatgcgggt tttatccccg agaatgctac cgccagtgac cgggctgctg
    17161 ggatcgaccg caataacagc aacctttaat ccctcacgaa tcaacaacat gccaaaggct
    17221 tcaagaaagg tacttttccc cgcgccgggt gtgccggtaa cgcccagtcg cagggcgtta
    17281 ccacagaacg gcataatagc gtcaagcagc tgtgtactta gcgcctgatg acgtgggtga
    17341 cggctttcca ccagcgtcat ggcttgggcg agcgtggcac gctcaccctg acgtaagcgg
    17401 cgaatacttt ctgccagcgt ggcttcatta atcatgatgc tggcttatca gattcagtac
    17461 gtcgcgcaca ctgtcgagca taggtgtacc tggaccataa atcgccgcca cgccgcgctc
    17521 ttgcaggaag gcgtaatcct gcggcggaat gacgccaccc gcgaccacgc agatatcttc
    17581 gcgtccccat tttttcagcg cttcgaccag ttccgggatc agcgttttat gaccggcagc
    17641 cagtgaggat gcgcccacta cgtgaacgtc gttttctacg gccaggcggg cgatctcttc
    17701 aggtgtagag aacatcgggc ttaaatctac gtcgaaaccg agatcggaat aggcgctggc
    17761 gatcactttc gcgccgcgat cgtgtccatc ctggcccatc ttagcgatca gaatgcgcgg
    17821 gcgacgacca ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat cgaactcgga
    17881 ggccgatttc tcagactgat gatagctttg cgcaatcacg ccggtaacac actggcttgg
    17941 caccagataa cggtcgaaag cgacttccag cgcatcggaa atttcaccca gggtggcgcg
    18001 aacgcgagcg gcattaacag cggcagccag caggttttcg ttatgctgtg cggcgtgagt
    18061 cagggcgttc aacgcggcgg ttacggcggc atcatcacgg gtggcgcgaa tgcgttccag
    18121 cgaagcaatt tgctcgttac gcaccatcac gttgtcgatc tcaagtacat cggtttcgtc
    18181 ttcgtgatcc agtttgtact tgttgacacc aacgatgaca cgcttgccct ggtcgatcag
    18241 cgactgttcg cgcgctgagg cctcttcgat cattcgtttt ggcagacctg cttcgatcgc
    18301 tttcgccatg ccaccggctt cgtcgatctg ttggataata gctctggctt gtttgacgat
    18361 ttgatcggtc agcgactcaa tgtaatagga tccggccagt ggatcgacgg tgtggcagag
    18421 ttctgattct tcctggatga tgatctgggt gttgcgggca atgcgtgctg agaaatcggt
    18481 aggcaaacca agcgcttcgt caaaggcgtt ggtatgcagt gactgagtac cgcccagcgt
    18541 cgcagccagt gcttcaatgg tggtgcggat aacgttgtta tacggatcct gttcagtcag
    18601 gctccagcct gaggtctggc agtgggtacg cagcgccagt gatttcggat cctgtgcgcc
    18661 aaatccactg accgcttcgc tccataaata acgtgccgca cgcaacatgg cgacgttcat
    18721 aaacagatcc atgccgatac cgaagaataa cgacaggcga ggagcgaagt catcaatttt
    18781 cagtccggca gagattgctg ctttgatgta ctcaatccca tcggcgatcg taaatgctac
    18841 ctgctgcacg cagttggcac ccgcttcccc catgtggtaa ccgctgatac tgatggtatt
    18901 aaatcgcggc atgttgccgg aacaccaggc gatgatgtcg gcgataatgc gcattgacgg
    18961 ttttggtggg taaatatagg tgttgcggca gaggtactct ttgagaatat cgttttgaat
    19021 ggtgccggtc agtttatcag gtgtaacacc ttgctcttct gcggcgacga tataaaacgc
    19081 cagtactggt agcactgcgc cattcatggt catcgaaacc gacattttat ccagcgggat
    19141 ctggtcgaac aggactttca tatcttccac ggtgtcgata gcgacgcccg ctttgccgac
    19201 gtcacccgcc acgcgtgggt tatcggagtc gtagccacgg tgggtggcaa ggtcaaacgc
    19261 aacggaaaga cctttttgcc cggcggccag gttacggcga taaaaagcgt tggactcttt
    19321 tgctgttgaa aaaccagcat actgacggat ggtccacggt tgggcggtat acatagtggc
    19381 acgcgggcca cgaacgtagg gcggcaaacc aggaagggta cctgtcacct ccagattatc
    19441 gagatcggct tcggtataca gcggcttgat ggcgatccct tccgcggttt gctgaaccag
    19501 cgagtcgaca gttttctccc gacggctcaa ttccttgttg gcaagctgtt gccactcctg
    19561 ctcgttagac attatttgct ccgtaaaacg caatcaaaat gagggtattt gcgcttacgg
    19621 tgagcttttt acgtgggttt gtcgccagca aaaaaaatgc attcagacgt cgttttttgc
    19681 cgcctgagat ttgtctattt tgtgatgtga ttcaaggaat acaaaaagaa gtgaggacaa
    19741 cgcctgatat gtgctatcag gcattatttg atggattaat cctgacgaag gactttgtga
    19801 ccataatcga gtaacgcatc agtgacttta cgcatcatgc ggctttcagg agcaaagcgg
    19861 tgccagtaga gcatccgtcg ttgaaatagc ccaggcgtta agtcaatcag ttcaccgctg
    19921 gccagctctt tctcgatttg caggtgcggg atcatacagc aggtggtgcc ctggcgagca
    19981 agttgtacga acgcttctga agaattaacg atatggcagg gtacgctgcc aggaggaaga
    20041 tcgaagtttt gctgcaaaaa ggcctggtgc atatcgtcaa gatggtcaaa cgcgaccact
    20101 ggcgctttca ataatgccga acgcgttacg ccgttaggaa aatatttttc ggcaaacggt
    20161 tttgagctga cgaacagata gtcgagcgca ccgagtttat cgacaagaca actcggcagc
    20221 gcctgatgtt gaatactcac cgcgccgacc acttcgccgc ggcgcagacg ttcctgagta
    20281 cgggtttcat cttctacctg caagttgagg cggataggcg aatcagccaa cacaggagcc
    20341 agtgccggaa gcaaccacgt cgccagactg tcggcgttga ccgccagtga aagcagcagc
    20401 ggagtcgaac cggtttgttc atcgcccagc cactcttctt ccagcaactc cacctggcgc
    20461 agcagtgcca gcagtttttg cccttgttcc gtcgggcgcg gcggtacggt acgcaccaac
    20521 agcggctgcc cgaacatatt ttccagttgc ttaatgcgct gtgagacggc tgattgtgta
    20581 atgcacagct tttgtgccgc gcgctcaaat cctcgttcac gtatcaccgc atccagtgcc
    20641 tgtaatgttc tgtagtccgg gcgtttcatt gctgttgtct gctcctggta aatgcttttt
    20701 cctgcactat gacacaattt tgtgtcaggt tgcatatacc gaagccgtcg gcgggtaaca
    20761 tttgtatcaa tggttaaacg cgcagcttaa aagatcaggg atttgcgcta ttgcatttcg
    20821 cttagcgact tcgcgccctg gttatgttga atggcgtggc gttattgcct ctgttcgcac
    20881 cgtggagcgg catacccaga ccgttgtatg ctttatttgc ggggcgaaag gggatgcccg
    20941 ccattgcgcg atattttatg gatgagttaa ccacccagct tgccaacggg gtctgaatcg
    21001 ctttttttgt atataatgcg tgtgaaattt cataccacag gcgaaacgat catgacgcag
    21061 gatgaattga aaaaagcagt aggatgggcg gcacttcagt atgttcagcc cggtaccatt
    21121 gttggtgtag gtacaggttc caccgccgca cactttattg acgcgctcgg cacgatgaaa
    21181 ggccagattg aaggggccgt ttccagttca gatgcttcca ctgaaaaact gaaaagcctc
    21241 ggcattcacg tttttgatct caacgaagtc gacagccttg gcatctacgt tgatggcgca
    21301 gatgaaatca acggccacat gcaaatgatc aaaggcggcg gcgcggcgct gacccgtgaa
    21361 aaaatcattg cttcggttgc ggaaaaattt atctgtattg ccgacgcctc caagcaggtt
    21421 gatattctgg gtaaattccc gctgccagta gaagttatcc cgatggcacg tagtgcagtg
    21481 gcgcgccagc tggtgaaact gggcggtcgt ccggaatacc gtcagggcgt ggtgaccgat
    21541 aatggcaacg tgatcctcga cgtccacggc atggaaatcc ttgacccgat agcgatggaa
    21601 aacgccataa atgcgattcc tggcgtggtg actgttggct tgtttgctaa ccgtggcgcg
    21661 gacgttgcgc tgattggcac acctgacggt gtcaaaacca ttgtgaaatg atctgacggg
    21721 ggaacctccc ccgttaaaaa aattctcttc attaaatttg gtgacatgtg tcacgctttt
    21781 accaggcaat tgtcgattgc tctaaataaa tcctctaaac cagcatattc atccaagaat
    21841 tacctttgcg tgatatttcc tcaacatcgc gacgcaaacg ttcatattgc cgcaatatta
    21901 ttttttgata tgttgaaagg cggatgcaaa tccgcacaca acatttcaaa agacaggatt
    21961 gggtaaatgg caaaggtatc gctggagaaa gacaagatta agtttctgct ggtagaaggc
    22021 gtgcaccaaa aggcgctgga aagccttcgt gcagctggtt acaccaacat cgaatttcac
    22081 aaaggcgcgc tggatgatga acaattaaaa gaatccatcc gcgatgccca cttcatcggc
    22141 ctgcgatccc gtacccatct gactgaagac gtgatcaacg ccgcagaaaa actggtcgct
    22201 attggctgtt tctgtatcgg aacaaaccag gttgatctgg atgcggcggc aaagcgcggg
    22261 atcccggtat ttaacgcacc gttctcaaat acgcgctctg ttgcggagct ggtgattggc
    22321 gaactgctgc tgctattgcg cggcgtgccg gaagccaatg ctaaagcgca ccgtggcgtg
    22381 tggaacaaac tggcggcggg ttcttttgaa gcgcgcggca aaaagctggg tatcatcggc
    22441 tacggtcata ttggtacgca attgggcatt ctggctgaat cgctgggaat gtatgtttac
    22501 ttttatgata ttgaaaacaa actgccgctg ggcaacgcca ctcaggtaca gcatctttct
    22561 gacctgctga atatgagcga tgtggtgagt ctgcatgtac cagagaatcc gtccaccaaa
    22621 aatatgatgg gcgcgaaaga gatttcgcta atgaagcccg gctcgctgct gattaatgcc
    22681 tcgcgcggta ctgtggtgga tattccggcg ctgtgtgatg cgctggcgag caaacatctg
    22741 gcgggggcgg caatcgacgt attcccgacg gaaccggcga ccaatagcga tccatttacc
    22801 tctccactgt gtgaattcga caacgtcctt ctgacgccac acattggcgg ttcgactcag
    22861 gaagcgcagg agaatatcgg cttggaagtt gcgggtaaat tgatcaagta ttctgacaat
    22921 ggctcaacgc tctctgcggt gaacttcccg gaagtctcgc tgccactgca tggtggtcgt
    22981 cgtctgatgc acatccacga aaaccgtccg ggcgtgctaa ctgcgctgaa caaaatcttc
    23041 gccgagcagg gcgtcaacat cgccgcgcaa tatctacaaa cttccgccca gatgggttat
    23101 gtagttattg atattgaagc tgacgaagac gttgccgaaa aagcgctgca ggcaatgaaa
    23161 gctattccgg gtaccattcg cgcccgtctg ctgtactaat tccccttctc tgaaaattaa
    23221 cgggcaggtc actgacttgc ccgttttttt atcccttctc cacaccgttg ccgcgttgcg
    23281 cattctactt tgcgagtctg ctccagaaaa taaatcaaag tcagatcaat gcgttgtgtt
    23341 tatttaatgg ggatttacga tggcgggaca gcatggggct gtaacggtaa agccctcacc
    23401 gaagcgaggg cttaaaggag aagggttatg atgcgacttg tcatcatact gattgtactg
    23461 ttactgataa gtttcggagc ttattaacag tcagtctcaa gggaggagtc atcctccctt
    23521 acccttactc actaaattag gtcaaagaat caacgatgtc aatcagggcg atgcggggtg
    23581 catcgccctg aaccgaactt tttgatagcc acttcatcgc ccacgtttac tgccgtaata
    23641 gatccttttt cccaaaaatt atctctgttt tgcatggggt ttttcgagca cgctcgaaga
    23701 aaagatttaa aggtagatca atgcgttgtg gttatttaaa gagagcttac gatagctggg
    23761 cagcatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttgaagg agaagggtta
    23821 tgatgcgact tgccatcata ctgattgtac tgttactcat aagtttccca gcttattaac
    23881 agtcagtcac aggggaggag caatcctccc ttacccttac tcactaaatt aggttaaaga
    23941 atcaacgatg tcaatcaggg cgatgcaggt gtatcgccct taccactccc agactttcga
    24001 cggtgtaacc accgcaggaa gagggatatc ccactcttca acggggagtt tttccaccaa
    24061 ctgacaatca tgcgcataac ccaccggttg cgttttatag tgctgccagt tttgtaaggt
    24121 ccgatcataa aaaccaccgc ccattcccag gcgctgaccg tactcatcaa aggcgaccag
    24181 cggtgtgatc agcacgtcta atcgggaaag gggtagcacg tcacgcacat ccagttttgg
    24241 ctcatggatc ttcaacctgt tcatcaccag ttcgctttgc ggatggtaat tcaggaacag
    24301 caaattaccg gcactaaagg gatgcaaaac tggaagatat acgcgcttac cggcgcgcca
    24361 gagttgttct attagcggct gggtgtcgag ttcaccatca aaagagagga atacagcgac
    24421 cgtatgcgcc atcaccaccg ggggataagt catcatccgg gtagcggctt gttgacccat
    24481 ttccagctgt tgttccggcg ttaacgcacg acgacgttgc cgaatcattt tgcggatttc
    24541 ttgtcgggat aatgtcagtg ggagctctgg tagttgcgtc atggctggtg ccagataaga
    24601 agggaatctc cgagatgccg ccgcaggctg taacccttga acccttggtt caaggtgaat
    24661 gtgtcgtcgc agttttaagg cttctcggac gaaccgagca tgctcaccaa ccgcggagcg
    24721 ccacattctt gtggtatgaa atatcggctc aggggactgg cccgcttgcg aacatctcag
    24781 agaaattttg tcttcacggt tactctacca cagtaaaccg aaaagtgtca ttcaaagttt
    24841 tggttagttt tttcggtgat gcgaccttgt tcaagtaacg cttgttctat ggtctgctgc
    24901 agcatccgaa tacgctgttc catacttgcc gcgtagtcac gagtctttgc tttttcttgc
    24961 gctaactcat agctgatatt caatgcggca atgaagacca actgttcagt atttgtgact
    25021 ctagtgcgtt ctttcagatc ttgcaaccgt tggttcagat cgtccgctgc ctgattcaac
    25081 gcatcccttt ggtcaggcgg gcagttcaca cgcagtgaac ggccaaaaat ttggatatcg
    25141 acgggttgtg cagacatgcc accttcctgc tgattgactg cgctgccttc gtctttagac
    25201 ccatggtctg cgaaggggcg acactatagc taccctgatg agaagagaca agcccttttc
    25261 tggtccacca gggcccaaag tggtagcata tcatgaatat tcctcccttt gacgacgaat
    25321 gcttatgtct atacagaacg aaatgcctgg ttacaacgaa atgaaccagt atctgaacca
    25381 acaagggacg ggtctgaccc ctgccgagat gcatggttta atcagtggga tgatatgtgg
    25441 cggtaacgat gacagctcat ggctgccgct acttcacgac ctgacgaacg aaggcatggc
    25501 tttcggtcat gagctggcac aggcactgcg taaaatgcac tctgccacca gcgatgccct
    25561 gcaggatgac ggcttccttt ttcagcttta tctgcctgat ggcgatgatg tcagcgtttt
    25621 cgatcgggct gatgcactgg caggttgggt caatcacttc ctgcttggtc ttggcgttac
    25681 gcaaccgaag ctggacaaag tgaccggcga aaccggtgaa gccatcgacg atctgcgtaa
    25741 catcgcgcag ctgggctacg acgaagacga agatcaggaa gagcttgaaa tgtcgcttga
    25801 agagatcatc gaatacgttc gtgttgccgc gctgttatgc cacgacacct ttactcatcc
    25861 gcaaccgacc gcgccagaag tacaaaaacc gactctacac taaaaacaaa aaacgtaagg
    25921 agagtgttat gagtgagata tcccggcaag agtttcagcg tcgccgtcag gccctggtgg
    25981 agcaaatgca acccggcagc gccgcgctga tttttgctgc accagaagta acacgtagcg
    26041 ccgacagcga atacccctat cgtcagaaca gtgacttctg gtacttcacc ggctttaacg
    26101 aaccggaagc ggtgctggtg ctgattaaaa gcgatgacac tcataaccac agcgttctgt
    26161 ttaaccgcgt tcgcgacctg acggcggaga tctggtttgg ccgtcgctta ggccaggatg
    26221 ccgcgccaga gaaactgggc gttgaccgcg cactggcatt cagcgaaatc aatcagcaac
    26281 tttatcaact acttaacggc ctggatgtgg tttaccatgc ccagggcgaa tatgcatatg
    26341 ctgatgaaat cgtgaacagt gcgctggaaa aactgcgtaa aggttcgcgg caaaatctca
    26401 ccgcaccggc aacgatgatc gactggcgtc ctgttgttca tgaaatgcgc ctgttcaaat
    26461 cgccagaaga gattgccgta ctccgccgcg cgggagaaat caccgccatg gcacatacac
    26521 gggcgatgga aaaatgccgt ccgggaatgt tcgagtacca tctggaaggc gaaattcacc
    26581 acgaatttaa ccgccacggt gcgcgctatc cttcctataa caccattgta ggcagcggtg
    26641 aaaacggctg cattctgcac tacaccgaaa acgagagtga actgcgcgac ggtgacctgg
    26701 tgttgattga cgcgggttgt gaatacaaag gttacgcggg cgatattacc cgcaccttcc
    26761 cggtcaacgg caaattcacc caggcccagc gtgaaatcta cgacattgtg ctggagtctc
    26821 tcgaaaccag cctgcgcctg tatcgtccgg gaacttccat tctggaagtc actggtgaag
    26881 tggtgcgcat catggttagc ggcctggtaa aactcggcat cctgaaaggt aatgttgatg
    26941 aactgatcgc tcagaacgcc catcgtcctt tctttatgca tggccttagc cactggttag
    27001 gactggatgt ccatgacgtt ggcgtttatg gtcaggatcg ctcgcgcatt ctggaaccgg
    27061 ggatggtact gaccgtagag cctggtctgt atatcgcgcc ggatgcggat gtgccagaac
    27121 aatatcgcgg tatcggcatt cgcattgaag acgacattgt gattaccgaa accggcaacg
    27181 aaaacctcac cgccagcgtg gtgaaaaagc cggaagaaat cgaagcgttg atggctgctg
    27241 cgagaaagca atgagcgtaa tcatcgtcgg tggcggcatg gcgggcgcga cgctggcgct
    27301 ggctatttcc cggttaagtc acggggcgct gccggtacat ttgattgaag cgactgcgcc
    27361 agagtcacat gctcatccgg gctttgatgg acgagcgata gcgctggcgg cgggtacctg
    27421 tcagcaactg gcgcgcatcg gcgtctggca atctctggct gattgcgcaa ccgccatcac
    27481 taccgtgcat gtcagcgatc gtggtcatgc tggattcgtc accctcgccg cagaagatta
    27541 ccaactggcg gcgctggggc aggttgtcga attgcataat gtcgggcaac ggctgtttgc
    27601 attgctgcga aaagcacctg gcgtaatgct gcattgcccc gatcgcgtag ctaacgttgc
    27661 ccgtactcag agtcatgttg aagtgacgct ggagagcggc gagatgctga cgggccgcgt
    27721 gctgatagcc gctgatggca cccattcagc gttagccacc gcgtgcggcg ttgattggca
    27781 gcaggagcct tacgaacaac tggcagtgat tgccaacgtt gctacttctg ttgcgcacga
    27841 aggccgcgct tttgaacgct tcacgcaaca tggcccgctg gcgatgttgc cgatgtctga
    27901 cggacgctgt tcgctggtct ggtgtcatcc actggaacgg cgcgaagagg tgctgtcgtg
    27961 gagcgacgag aagttttgcc gtgaactcca gtctgccttt ggctggcgac tggggcaaat
    28021 tacccacgcc ggtaaacgca gtgcttatcc gctggcatta acccgcgccg ccagaccgat
    28081 tacccatcgc actgtgctgg tgggcaatgc ggcgcaaact ctgcacccga ttgccgggca
    28141 agggtttaac ctcggtatgc gagatgtcat gagccttgcg gaaaccctga ctcaggcgca
    28201 tgagcgcgga gaagacatcg gtgattacgg catattgtgc cgctatcagc agcgtcgaca
    28261 gagcgatcgc gaagcaacca tcggcgtcac ggacagcctg gtacatcttt ttgccaaccg
    28321 ttgggcaccg ctggttgtcg ggcgcaacat cggactgatg acgatggaat tattcacccc
    28381 ggcacgcgat gtgctggcgc agcgcaccct cggttgggtg gcgcgttgaa ggcggtttta
    28441 aggaataaac aatgcaaagt gttgatgtag ccattgttgg cggcggcatg gtggggctgg
    28501 cggttgcctg cggcttacag gggagcggct tacgcgttgc cgtactggag cagcgcgtac
    28561 cggaacctct ggcggtagat gcactaccac aactgcgcgt ttcggctatc aatgccgcca
    28621 gcgaaaaatt actcacccgt cttggcgtct ggcaggacat tctcgctcgt agggccagtt
    28681 gttatcacgg catggaagtg tgggacaaag acagttttgg tcacatctcg tttgacgatc
    28741 aaagcatggg ctatagccat cttgggcaca tcgttgaaaa ctcagtgatt cactacgcgc
    28801 tgtggaacaa agcgcagcag tcgtcagata ttactctgtt ggcccccaca gaattacagc
    28861 aggtcgcctg gggagaaaat gaaaccttcc tgacgctgaa agatggcagc atgttaacgg
    28921 cgcgtctggt gattggcgcg gacggtgcta attcctggtt gcgcaacaaa gcagatattc
    28981 cgctgacttt ctgggattat cagcatcacg cgctggtagc gaccattcgc acggaagaac
    29041 cgcatgatgc ggtggcgcgg caggttttcc atggcgaagg cattctggcc tttttaccgc
    29101 ttagcgatcc gcatctttgc tcgattgtct ggtcactgtc gccagaggaa gcgcagcgga
    29161 tgcagcaggc aagtgaagac gaatttaatc gcgcgttaaa tatcgctttt gataatcgcc
    29221 ttggcttatg caaggttgag agcgcgcgtc aggtgttccc gctgacgggg cgttatgcgc
    29281 gccagtttgc cgcacaccgt ctggcgctgg tgggcgacgc cgcgcatacc attcacccac
    29341 tggcggggca gggcgttaat ctcggattta tggacgccgc agagctgatt gctgaactga
    29401 aacggttgca tcgtcagggt aaagacatcg ggcagtacat ttacctgcgc cgctatgaac
    29461 gtagccgcaa gcacagcgcg gcgctgatgc tggctggtat gcagggtttt cgcgatctct
    29521 tttccggcgc taatccggcg aaaaaactgc tgcgtgatat tggtttgaaa ctggccgaca
    29581 cgcttcctgg cgttaagccg caacttatcc gccaggcaat gggattaaac gatttgcctg
    29641 aatggctgcg ttaaaaattt ctcctctgtt gtttatttga tacccatcac actttcatct
    29701 cccggttttt tcgccgggag attttcctca tttgaaataa actaatttca cctccgtttt
    29761 cgcattatat tttctaatac catgattttt tgatttagtg ttttttgaca tttttttagc
    29821 tcttaatatt gtcttattca aattgacttt ctcatcacat catctttgta tagaaattgg
    29881 tgtatttttt ggttttttat tctgtcgcga tttttgcatt ttttaaccat aagctaatgt
    29941 gatgatcaat tttaccttat ggttaacagt ctgtttcggt ggtaagttca ggcaaaagag
    30001 aacgattgcg ttggggaccg ggagtggctc cgatgctggg ttttgtggtg ataatttcac
    30061 catgaaaaag ttgtcagccc cgcttattca atgaggacaa gatggcacaa cagactcctt
    30121 tgtacgaaca acacacgctt tgcggcgctc gcatggtgga tttccacggc tggatgatgc
    30181 cgctgcatta cggttcgcaa atcgacgaac atcatgcggt acgtaccgat gccggaatgt
    30241 ttgatgtgtc gcatatgacc atcgtcgatc tccgcggcag ccgcacccgg gagtttctgc
    30301 gttatctgct ggcgaacgat gtggcgaagc tcaccaaaag aggcaaagcc ctttactcgg
    30361 ggatgttgaa tgcctctggc ggtgtgatag atgatctcat cgtctactac tttactgaag
    30421 atttcttccg cctcgttgtt aactccgcca cccgcgaaaa agacctctcc tggattaccc
    30481 aacacgctga acctttcggc atcgaaatta ccgttcgtga tgacctttcc atgattgccg
    30541 tgcaagggcc gaatgctcag gcaaaagctg ccacactgtt taatgatgct cagcgtcagg
    30601 cggtggaagg gatgaaaccg ttctttggcg tgcaggtggg cgatctgttt attgccacca
    30661 ctggttatac cggtgaagcg ggctatgaaa ttgcgctgcc caatgaaaaa gcggccgatt
    30721 tctggcgtgc gctggtggaa gcgggcgtta agccctgcgg tctgggggcg cgtgacacgc
    30781 tgcgtctgga agcgggcatg aatctttatg gtcaggagat ggacgaaact atttctcctt
    30841 tagccgccaa catgggctgg actatcgcct gggaaccgac agatcgtgac tttatcggtc
    30901 gtgaagccct ggaagtgcag cgtgagcatg gtacagaaaa actggttggt ctggtgatga
    30961 ccgaaaaagg cgtgctgcgt aatgaactgc cggtacgctt taccgatgcg cagggcaacc
    31021 agcatgaagg cattatcacc agcggtactt tctccccgac gctgggttac agcattgcgc
    31081 tggcgcgcgt gccggaaggt attggcgaaa cggcgattgt gcaaattcgc aaccgtgaaa
    31141 tgccggttaa agtgacaaaa cctgtttttg tgcgtaacgg caaagccgtc gcgtgattta
    31201 cttttttgga gattgattga tgagcaacgt accagcagaa ctgaaataca gcaaagaaca
    31261 cgaatggctg cgtaaagaag ccgacggcac ttacaccgtt ggtattaccg aacatgctca
    31321 ggagctgtta ggcgatatgg tgtttgttga cctgccggaa gtgggcgcaa cggttagcgc
    31381 gggcgatgac tgcgcggttg ccgaatcggt aaaagcggcg tcagacattt atgcgccagt
    31441 aagcggtgaa atcgtggcgg taaacgacgc actgagcgat tccccggaac tggtgaacag
    31501 cgaaccgtat gcaggcggct ggatctttaa aatcaaagcc agcgatgaaa gcgaactgga
    31561 atcactgctg gatgcgaccg catacgaagc attgttagaa gatgagtaac ggctttattc
    31621 ctcttctgcg ggagaggatc agggtgagga aaatttatgc ctcaccctca ctctcttcgt
    31681 aaggagagag gttcacaatt cactgcacgt ttcaggaacc atcgctcatg acacagacgt
    31741 taagccagct tgaaaacagc ggcgctttta ttgaacgcca tatcggaccg gacgccgcgc
    31801 aacagcaaga aatgctgaat gccgttggtg cacaatcgtt aaacgcgctg accggccaga
    31861 ttgtgccgaa agatattcag cttgcgacac caccgcaggt tggcgcaccg gcgaccgaat
    31921 acgccgcact ggcagaactc aaggctattg ccagtcgcaa taaacgcttc acgtcttaca
    31981 tcggcatggg ttacaccgcc gtgcagctac cgccggttat cctgcgtaac atgctggaaa
    32041 atccgggctg gtataccgcg tacactccgt atcagcctga agtctcccag ggccgccttg
    32101 aagcactgct caacttccag caggtaacgc tggatttgac tggactggat atggcctctg
    32161 cttctcttct ggacgaggcc accgctgccg ccgaagcaat ggcgatggcg aaacgcgtca
    32221 gcaaactgaa aaatgccaac cgcttcttcg tggcttccaa tgtgcatccg caaacgctgg
    32281 atgtggtccg tactcgtgct gaaacctttg gttttgaagt gattgtcgat gacgcgcaaa
    32341 aagtgctcga ccatcaggac gtcttcggcg tgctgttaca gcaggtaggc actaccggtg
    32401 aaattcacga ctacactgcg cttattagcg aactgaaatc acgcaaaatt gtggtcagcg
    32461 ttgccgccga tattatggcg ctggtgctgt taactgcgcc gggtaaacag ggcgcggata
    32521 ttgtttttgg ttcggcgcaa cgcttcggcg tgccgatggg ctacggtggc ccacacgcgg
    32581 cattctttgc ggcgaaagat gaatacaaac gctcaatgcc gggccgtatt atcggtgtat
    32641 cgaaagatgc agctggcaat accgcgctgc gcatggcgat gcagactcgc gagcaacata
    32701 ttcgtcgtga gaaagcgaac tccaacattt gtacttccca ggtactgctg gcaaacatcg
    32761 ccagcctgta tgccgtttat cacggcccgg ttggcctgaa acgtatcgct aaccgcattc
    32821 accgtctgac cgatattctg gcggcgggcc tgcaacaaaa aggtctgaaa ctgcgccatg
    32881 cgcactattt cgacaccttg tgtgtggaag tggccgacaa agcgggcgta ctgacgcgtg
    32941 ccgaagcggc tgaaatcaac ctgcgtagcg atatcctgaa cgccgtgggg atcacccttg
    33001 atgaaaccac cacgcgtgaa aacgtaatgc agcttttcag tgtgctgctg ggcgataacc
    33061 acgggctgga gatcgacacg ctggacaaag acgtggctca cgacagccgc tctatccagc
    33121 ctgcgatgct gcgcgacgac gaaatcctca cccatccggt gtttaatcgc tatcacagcg
    33181 aaaccgaaat gatgcgctat atgcactcgc tggagcgtaa agatctggcg ctgaatcagg
    33241 cgatgatccc gctgggttcc tgcaccatga aactgaacgc cgccgccgag atgatcccaa
    33301 tcacttggac ggaatttgcc gaactgcacc cgttttgccc gccggaacag gctgaaggtt
    33361 atcagcagat gattgcgcag ctggctgact ggctggtgaa actgaccggt tacgacgccg
    33421 tttgtatgca gccgaactcc ggcgcacagg gcgaatacgc gggcctgctg gcgattcgtc
    33481 attatcatga aagccgcaac gaagggcatc gcgatatctg cctgatcccg gcttctgcgc
    33541 acggaactaa ccccgcttct gcacatatgg caggaatgca ggtggtggtt gtggcgtgtg
    33601 ataaaaacgg caacatcgat ctgactgatc tgcgcgcgaa agcggaacag gcgggcgata
    33661 acctctcctg tatcatggtg acttatcctt ctacccacgg cgtgtatgaa gaaacgatcc
    33721 gtgaagtgtg tgaagtcgtg catcagttcg gcggtcaggt ttaccttgat ggcgcgaaca
    33781 tgaacgccca ggttggcatc acctcgccgg gctttattgg cgcggacgtt tcgcacctca
    33841 acctgcataa aactttctgc attccgcacg gcggtggtgg tccgggtatg ggaccgatcg
    33901 gtgtgaaagc gcatctggca ccgtttgtac cgggtcatag cgtggtgcaa atcgaaggca
    33961 tgttaacccg tcagggcgca gtttctgcgg caccgttcgg tagcgcctct atcctgccaa
    34021 tcagctggat gtacatccgc atgatgggcg cagaagggct gaaaaaagca agccaggtgg
    34081 caatcctcaa cgccaactat attgccagcc gcctgcagga tgccttcccg gtgctgtata
    34141 ccggtcgcga cggtcgcgtg gcgcacgaat gtattctcga tattcgcccg ctgaaagaag
    34201 aaaccggcat cagcgagctg gatattgcca agcgcctgat cgactacggt ttccacgcgc
    34261 cgacgatgtc gttcccggtg gcgggtacgc tgatggttga accgactgaa tctgaaagca
    34321 aagtggaact ggatcgcttt atcgacgcga tgctggctat ccgcgcagaa attgaccagg
    34381 tgaaagccgg tgtctggccg ctggaagata acccgctggt gaacgcgccg cacattcaga
    34441 gcgaactggt cgccgagtgg gcgcatccgt acagccgtga agttgcggta ttcccggcag
    34501 gcgtggcaga caaatactgg ccgacggtga aacgtctgga tgatgtttac ggcgaccgta
    34561 acctgttctg ctcctgcgta ccgattagcg aataccagta attcactgat tcgagtattt
    34621 tctaaaggcg cttcggcgcc tttttagtca gatgacaaag tacaaaagtg ctcagacagt
    34681 cccctcgccc ctttggggag agggttaggg tgaggggaac aggccggcac tggcgcgaat
    34741 atttaccctc accccgaccc tctccctgaa agggcgaggg ggaaaagcgt gccaacattg
    34801 aagattgagc cagtttgtta gcaatctcaa agatacgtca acgaattaat ttttctcgga
    34861 aaaacaaatg gctatagcac ttgtgaccgg tggcagtcgc ggcatcgggc gggcaactgc
    34921 attactgttg gcgcaagaag ggtatacggt ggcggttaat tatcagcaaa acctccacgc
    34981 ggcgcaggaa gtgatgaact taataatgca agccggtggc aaagcattcg tgctccaggc
    35041 ggatatcagc gacgaaaacc aggtcgttgc gatgtttaca gcaatcgatc agcacgatga
    35101 accgctagca gcgctggtca ataacgccgg gatcttgttt acccagtgca ccgttgaaaa
    35161 ccttaccgca gagcgaatca accgagtact ttccaccaac gtgacgggat attttctctg
    35221 ctgccgcgag gcggtaaaac gcatggcgct taaaaatggt ggcagtggcg gcgctatcgt
    35281 caatgtctct tcggtggcct cacggttggg ttcgccaggg gaatatgttg attacgcggc
    35341 atcgaaaggg gcgattgatt cgttaaccac cggactatcg ctggaagtcg ccgcgcaggg
    35401 gatccgcgtt aactgcgtgc ggccagggtt tatttatacc gaaatgcacg ccagcgcggc
    35461 gagcctggac gcgtcgatcg cgttaagtcg aacatcccca tgcagcgtgg tggacaggca
    35521 gaagaggtcg cgcaggccat tgtctggcta ctaagtgata aagcctctta cgtcacggga
    35581 agttttatcg atttggcggg cgggaaataa aacagggaag ttgtctgacc ggatgcaaca
    35641 agtattgcat ccggtacttc atcgacttaa agcttctcgc cgttgctggc aatcacctct
    35701 ttgtaccagt taaagctctt cttacgtgaa cgcgacatat cgccagtacc gtcgtcatgt
    35761 ttattcacat agataaagcc gtagcgtttg ctgtactgcc cggtagtgaa tgacacgcag
    35821 tcgatgcagc cccacggtgt gtagcccatc agatccacac catcgtaagt cactgctttt
    35881 ttcatctctt caatatgggc gcgcaggtag tcaatgcggt agtcgtcgtt gatgctgcca
    35941 tcttcttcca ctttgtcgta agcgccaaaa ccgttttcga caataaacag cggcctctga
    36001 taacgctcat acagttcgca aagggcatag cgcagaccta ctggatcaat ctgccagccc
    36061 cagtcagacg ctttaacata cgggttcggt acgctgcctt caaaaccaga gatcgcatcg
    36121 ccggtgccgc cttcggcctt cactgcgttg gtcatgtaat agctgaaacc aagataatcg
    36181 caggtgcctt cgcgcagtac atcgagatcg ccgtcttcca ttttgatatt aaatccgcga
    36241 cgttcccact cgttcaatac ataggacggg taatagccgc atagctgcac atcggtaaag
    36301 acgtagcgtt cgcgcatcga ctcctgagcg aacatcacat cgtccgggtt acatgagtag
    36361 ggatagagcg gcaccatcgc cagcatacag ccgactttca tctccgggtt aatgcgacgc
    36421 gcggctttca ctgccagggc gctggcgaca aactggtgat gcagcacctg gtacatcgtc
    36481 tcttccgggt tgtcatgctc ggtatacacc acgccggagc agcagtaacc aaacagcggc
    36541 gcacgccagt tacgctggtt gtttatttcg ttgaaggtca tccagtattt gactttatgc
    36601 ttgtagcgtt cgaataccac ttccgcgaaa cggacaaaga aatcaacgaa tttacggttg
    36661 gtccagctac cgtattgctg caccagatgc agcggcattt caaagtggga gagggttatc
    36721 accggttcga tgttgtattt cagcagttca tcgaacagag agtcgtaaaa cttcagccct
    36781 tcttcgtttg gctgagcttc atcacctttt ggaaaaatac gcgtccaggc gatggaagtc
    36841 cgaaaacatt tgaagcccat ttcggcaaat agcttgatgt cttccttgta gtgaccataa
    36901 aaatcaacgg cttcatggtt tggatagtat tttcctggca agacttcttt ggtgatttcg
    36961 cgcggcacgc cgtgtgcgcc accggtcaga acgtcacaaa tgctcggtcc tttgccgcct
    37021 ttgttccagc cgccttcgac ctgatgagcg gcaacggcgc caccccataa gaaatctttc
    37081 ggtaaggtga gttttttcac tatcataggc tccagatcag tcaattctta caggaaatag
    37141 taccatgcag ccaaacgaca tcactttttt caacgtttcc aggatgacat tctggctggg
    37201 cgtaaaacca tcaccatccg cgacgagtct gaatcgcact tcaaaacggg tgatgtgctt
    37261 cgtgtcggac gttttgaaga tgacggttat ttttgcacga ttgaagtcac cgcaacctca
    37321 accgtaacgc tggatacact gacggaaaaa catgcagagc aggaaaatat gaccctgcct
    37381 gaactgaaaa aggtcattgc tgacatctat cccggtcaga cacaatttta tatgattgaa
    37441 tttaaatgtc tttaaattga actcacgcct gttagttgaa atttcgcatt taatttcatg
    37501 atttttagga gattagtctc aaatcagaat ttattttagc taacaggtgt tcactggaac
    37561 tattctcagt tacgctggag aggtatacat gtgcgtgtac gtttctctgg agtaagttat
    37621 ggttcagaag cccctcatta agcagggata ttcgctggca gaggaaattg ccaacagcgt
    37681 cagtcacggc attgggctgg tgtttggtat cgttgggctg gtgttgctac tggttcaggc
    37741 ggtggatctc aatgccagcg ccacagcgat aaccagttac agcctctatg gcggcagtat
    37801 gatcctgctg ttcctcgctt cgacgctcta tcacgccatt cctcatcaac gggcaaaaat
    37861 gtggctgaag aaatttgacc attgcgctat ttacctgttg attgccggaa cctacacgcc
    37921 gtttttgctg gtggggctgg attctccgtt agcgcgcggg ttgatgattg ttatctggag
    37981 cctagcattg ctggggattc tgttcaaact gaccatcgcg caccgattca aaattttatc
    38041 tctggtgact tatctggcga tgggctggct gtcgctggtg gtaatttatg aaatggcagt
    38101 taagctcgcg gcgggcagcg ttaccttact ggcggtaggc ggcgtggttt attcgctcgg
    38161 ggtgattttc tacgtctgca aacgcattcc atacaaccat gccatctggc acggcttcgt
    38221 gctcggcggt agtgtgtgcc actttctggc gatctatttg tatattgggc aggcgtaatg
    38281 ggtatgcctt tgtaggcctg ataagacgcg tcagcgtcgc atcaggcatc ggagcgcaac
    38341 cgccggatgc ggcgtgaacg ccttatccga tctacgggcc tcgtccctgt tgtgataaag
    38401 gcatcaaatc ccagacatga taagacgcgt cagcgtcgca tcaggcgata aatgcctgat
    38461 gcgatacaga gatttactct tcgagcgaat acggcagcgg ctcgatatgc agtgtattcg
    38521 catcgtcgcg tacgcggaag atgctgtccg gttccatatc gttattcatg acgacctgta
    38581 ccacgacctg accatcttcc agttttaccg cagccagcac cgtaccggta cgacgccagt
    38641 tctcgcccat tttcagctct aagtcttcac cagcttccgg cagtcggctg acactacctg
    38701 ccagcaacca gagcgcgcgt ttgttggcac cacggaattt tgctcgcgcc accatctctt
    38761 gtccggtata acagcctttc ttaaagctga taccgcccag cgcctggaga ttggtcgcct
    38821 gtgggataaa ctgcccgctg ttggcggcat caatcaccgg gaaaccagct tcaatgttta
    38881 atgccagcca ctgttggcta ttgttcagtt ccgcttcacc gcgcagttta tcggtcagca
    38941 tattagcagt agcttcatcg gttacgatca ggaaacgttc tgccgggtgt tcaaaccata
    39001 gcaaagtggt cgcgccttct ttgactacct gtttttcttt cgaaggcagt tcgctaaaga
    39061 gatttgccag cgcggcgcgc gcctgaaaac cggcaacacc aagcagcaca cgctcgtcgt
    39121 ctggcgcgat ggtcacttta gagaataccg catatttttt cagttcagtc agctgcggtt
    39181 cacgcacgct gcgccgttca atccatgcaa agccatcgcc gtcgcggaac agacgtaaat
    39241 tgctccacat tttacctttg gcgtcgcaat gggcggcgag caggtgctga tcttctgcca
    39301 tctggctgac atctgctgtc acctgaccct gcatatattt ttcgctgtcc gcgccagtaa
    39361 tggtggcaag cgcccagtca tcaagcgtca tcagcgtcag cggtaaacgg gcagaagccg
    39421 taggctgacg tggaggaaaa ggtgtaaaag ccataataga gtcctgagtt gcttaacggc
    39481 gtattaatga ctaatggtaa aagagctatg aggcaatgca agtgctttca ctacgcgatc
    39541 tgtgccttct gcgaggcgac tgccaaaaaa atgaagccct tgaattgttg tgaattaatt
    39601 tcgcgatcgc gatggtgttt actgatattc ccgtaaaaga agtcgaaaaa cacgttacaa
    39661 tagccgggta ctggattttc gtgagacagg aagaagatgg acattaacaa caaagcccga
    39721 attcattggg catgccgccg tggtatgcgc gaactcgata tttcaatcat gccgtttttc
    39781 gaacatgagt acgacagctt aagcgatgac gaaaaacgca tctttattcg tctgctggaa
    39841 tgtgacgatc cggacctgtt taactggctg atgaatcacg gtaaaccggc cgatgcagaa
    39901 ctggaaataa tggtccgact catccagaca cggaaccggg aacgtggtcc tgtggcaatc
    39961 tgatttgcgc gtctcctggc gcgcacagtg gctttccttg ctgattcatg ggctggttgc
    40021 cgctgttatt ttactcatgc cctggccgct cagttacacc ccgttatgga tggtgttact
    40081 ttcgctggtg gtatttgatt gcgttcgcag ccagcggtgt attaatgctc gccaggggga
    40141 aattcgcttg ttgatggacg ggcgtttgcg ttggcaaggg caggagtgga gcatcgtcaa
    40201 agcaccgtgg atgattaaga gcggcatgat gctgcgttta cgttctgatg gcggtaaacg
    40261 gcaacattta tggctggcag ccgacagcat ggacgaagcc gaatggcggg atttacggcg
    40321 gattttgttg caacaagaga cgcaaagata aagatgacgg caaagcatat gcgctaagcc
    40381 gtcagtgaga tcaggcgtaa tgctctgcca tttcgttgag gatttgctcg caccagctct
    40441 gaatacgctc gtcgctaagg tcatactggt tagtttcatc cagcgccaga cccacgaaca
    40501 gttgcccgtc agcaatcacc ggtttcgggc tggtaaattc atatccttcc gttggccagt
    40561 agccgacgaa cttcacgcct ttggtcgaga gtttgtcatg cagcataccg agcgcatcga
    40621 ggaaccactc gccgtatccc agttgatcgc caagcccata cagcgcaata attttacctt
    40681 caaggttcag gtcgtcgagc tgatcccaga cggcttccca gtcttcctgg atttcaccaa
    40741 aatcccaggt cgggataccc agaatgagca catcgtactg ctccattaat ttcggggagt
    40801 cgtccttgag gttatgtaag gtcaccagtt ctgggccgat aatatcgcgg attttttctg
    40861 ccgccatttc ggtgtaacag gtgctggaac cgtaaaaaag acccatattc ataacgtaag
    40921 cttctcgatt ctgctgtggc ttttgcagct agtgtatcag aatcgcttta gtcacggcac
    40981 aataaggcat aatggatcaa atgagtgacc ataaggggcg caagtgaaac aggagctggc
    41041 acgcatcgag cagtttcttg atgctctgtg gctggaaaaa aatctggctg aaaatacgtt
    41101 gaacgcttac cgtcgcgatc tgtcaatgat ggtggagtgg ttgcatcacc gcgggttgac
    41161 gctggcgacg gcgcaaagtg acgatttgca ggcattactg gcagaacggc tggagggcgg
    41221 gtataaagcc accagctcag cgcgtttgct gagtgcagtg cgccgattgt tccagtatct
    41281 ttatcgcgaa aagtttcgtg aagacgatcc cagtgcgcat ctcgcttcac cgaaattgcc
    41341 ccagcgtttg ccaaaagatt taagcgaagc gcaggtcgaa cgtttattac aggcgccatt
    41401 aattgatcag ccactggagc tacgcgataa agccatgctt gaagtgttgt atgctaccgg
    41461 gctgcgtgtc tctgaactgg tcgggctgac aatgagtgat atcagcctgc gtcagggcgt
    41521 agtgcgggtc attggtaaag gcaacaaaga gcgcctggtg ccgttagggg aagaggcggt
    41581 ttactggctg gaaacctatc tggaacatgg gcgtccgtgg ctgctgaatg gtgtgtcaat
    41641 tgacgtgttg tttcccagcc agcgtgcgca gcagatgacg cgacagacct tctggcaccg
    41701 cattaaacat tatgccgtgc tggcgggtat cgacagcgaa aagctgtcac cgcatgtgtt
    41761 gcgtcacgct tttgccactc atttattaaa tcatggtgcg gatttacgcg tggtgcagat
    41821 gctactgggc cacagcgatc tttccaccac gcaaatttat acgcatgtcg ctaccgagcg
    41881 tctgcggcaa cttcatcaac agcatcaccc gcgggcgtga tgtctgaaaa gaacgggaag
    41941 atttatgaag aaaggtttta tgttgtttac tttgttagcg gcgttttcag gctttgctca
    42001 ggctgatgac gcggcaattc aacaaacgtt agccaaaatg ggcatcaaaa gcagcgatat
    42061 tcagcccgcg cccgtagctg gcatgaagac agttctgact aacagcggtg tgttgtacat
    42121 caccgatgat ggtaaacata tcattcaggg gccaatgtat gacgttagtg gcacggctcc
    42181 ggtcaatgtc accaataaga tgctgttaaa gcagttgaat gcgcttgaaa aagagatgat
    42241 cgtttataaa gcgccgcagg aaaaacacgt catcaccgtg tttactgata ttacctgtgg
    42301 ttactgccac aaactgcatg agcaaatggc agactataac gcgctgggga tcaccgtgcg
    42361 ttatcttgct ttcccgcgcc aggggctgga cagcgatgca gagaaagaaa tgaaagctat
    42421 ctggtgtgcg aaagataaaa acaaagcgtt tgatgatgtg atggcaggta aaagcgtcgc
    42481 accagccagc tgcgacgtgg atattgccga ccattacgca cttggcgtcc agcttggcgt
    42541 tagcggtact ccggcagttg tgctgagcaa tggcacactt gttccgggtt accagccgcc
    42601 gaaagacatg aaagaattcc tcgacgaaca ccaaaaaatg accagcggta aataattcgc
    42661 gtgaaacaac agatacaact tcgtcgccgt gaagtcgatg aaacggcaga cttgcccgct
    42721 gaattgcctc ccttgctgcg ccgtttatac gccagccggg gagtacgcag tgcgcaagaa
    42781 ctggaacgca gtgttaaagg tatgttgccc tggcagcaac taagcggtgt cgaaaaggcc
    42841 gttgagatcc tttacaacgc ttttcgcgaa ggaacgcgga ttattgtggt cggtgatttc
    42901 gacgccgacg gcgcgaccag cacggctcta agcgtgctgg cgatgcgctc gcttggttgc
    42961 agcaatatcg actacctggt accaaaccgt ttcgaagacg gttacggctt aagcccggaa
    43021 gtggtcgatc aggcccatgc ccgtggcgcg cagttaattg tcacggtgga taacggtatt
    43081 tcctcccatg cgggagttga gcacgctcgc tcgttgggca tcccggttat tgttaccgat
    43141 caccatttgc cgggcgacac attacccgca gcggaagcga tcattaaccc taacttgcgc
    43201 gactgtaatt tcccgtcgaa atcactggca ggcgtgggtg tggcgtttta tctgatgctg
    43261 gcgctgcgca cctttttgcg cgatcagggc tggtttgatg agcgcggcat cgcaattcct
    43321 aacctggcag aactgctgga tctggtagca ctggggacag tagcggacgt cgtgccgctg
    43381 gacgctaata atcgcattct gacctggcag gggatgagtc gcatccgtgc cggaaagtgc
    43441 cgtccaggga ttaaagcgct gctggaagtg gcaaatcgcg atgcacaaaa actagccgcc
    43501 agcgatttag gttttgcgct gggaccgcgt ctcaatgctg ccgggcgact ggatgatatg
    43561 tccgttggtg tggcgctgtt gctgtgcgac aacatcggcg aagcgcgcgt gctggcaaat
    43621 gaactcgatg cgctaaacca gacgcgaaaa gagatcgaac aaggaatgca ggttgaagcc
    43681 ctgaccctgt gcgagaaact ggagcgcagc cgtgacacgc tacccggcgg gctggcaatg
    43741 tatcaccccg aatggcatca gggcgttgtc ggtattctgg cttcgcgtat caaagagcgt
    43801 tttcaccgtc cggttatcgc ctttgcgcca gcaggtgacg gtacgctgaa aggttccggt
    43861 cgctccattc aggggctgca tatgcgtgat gcgctggagc gattagacac actctacccc
    43921 ggcatgatgc tcaagtttgg cggtcatgcg atggcggcgg gtttgtcgct ggaagaggat
    43981 aaattcgaac tctttcaaca acggtttggc gaactggtta ctgagtggct ggccccttcg
    44041 ctattgcaag gcgaagtggt gtcagacggt ccgttaagcc cggccgaaat gaccatggaa
    44101 gtggcgcagc tgctgcgcga tgctggcccg tgggggcaga tgttcccgga gccgctgttt
    44161 gacggtcatt tccgtctgct gcaacagcgg ctggtgggcg aacgtcattt gaaggtgatg
    44221 gtcgaaccgg tcggcggcgg tccactgctg gatggtattg cttttaatgt cgataccgct
    44281 ctctggccgg ataacggcgt gcgcgaagtg caactggctt ataagctcga tatcaacgag
    44341 tttcgcggca accgcagcct gcaaattatc atcgacaata tctggccaat ttagcgtcat
    44401 cttctctata aaaaagagcg tggattgggt acaatcccgc tcttatcacc gcattttgac
    44461 tagctcaata aaagaaatca gaccatgttt gaaattaatc cggtaaataa tcgcattcag
    44521 gacctcacgg aacgctccga cgttcttagg gggtatcttt gactacgacg ccaagaaaga
    44581 gcgtctggaa gaagtaaacg ccgagctgga acagccggat gtctggaacg aacccgaacg
    44641 cgcacaggcg ctgggtaaag agcgttcctc cctcgaagcc gttgtcgaca ccctcgacca
    44701 aatgaaacag gggctggaag atgtttctgg tctgctggaa ctggctgtag aagctgacga
    44761 cgaagaaacc tttaacgaag ccgttgctga actcgacgcc ctggaagaaa aactggcgca
    44821 gcttgagttc cgccgtatgt tctctggcga atatgacagc gccgactgtt acctcgatat
    44881 tcaggcgggg tctggcggta cggaagcaca ggactgggcg agcatgcttg agcgtatgta
    44941 tctgcgctgg gcagaatcgc gtggtttcaa aactgaaatc atcgaagagt cggaaggtga
    45001 agtggcgggt attaaatccg tgacgatcaa aatctccggc gattacgctt acggctggct
    45061 gcgtacagaa actggcgttc accgcctggt gcgtaaaagc ccgtttgact ccggcggtcg
    45121 tcgccacacg tcgttcagct ctgcgtttgt ttatccggaa gttgatgatg atattgatat
    45181 cgaaatcaac ccggcggatc tgcgcattga cgtttatcgc gcgtccggcg cgggcggtca
    45241 gcacgttaac cgtaccgaat ctgcggtgcg tattacccac atcccgaccg ggatcgtgac
    45301 ccagtgccag aacgaccgtt cccagcacaa gaacaaagat caggccatga agcagatgaa
    45361 agcgaagctt tatgaactgg agatgcagaa gaaaaatgcc gagaaacagg cgatggaaga
    45421 taacaaatct gacatcggct ggggcagcca gattcgttct tatgtccttg atgactcccg
    45481 cattaaagat ctgcgcaccg gggtagaaac ccgcaacacg caggccgtgc tggacggcag
    45541 cctggatcaa tttatcgaag caagtttgaa agcagggtta tgaggaacca acatgtctga
    45601 acaacacgca cagggcgctg acgcggtagt cgatcttaac aatgaactga aaacgcgtcg
    45661 tgagaagctg gcgaacctgc gtgagcaggg aattgccttc ccgaacgatt tccgtcgcga
    45721 tcatacctct gaccaattgc acgcagaatt cgacggtaaa gagaacgaag aactggaagc
    45781 gctgaacatc gaagtcgccg ttgctggccg catgatgacc cgccgtatta tgggtaaagc
    45841 gtctttcgtt accctgcagg atgtgggtgg ccgcattcag ctgtacgttg cccgtgacga
    45901 tctgccggaa ggcgtttaca acgagcagtt caaaaaatgg gacctcggcg acatcctcgg
    45961 cgcgaaaggt aaactgttca aaaccaaaac cggcgaactg tctatccact gtaccgagct
    46021 gcgtctgctg accaaagcac tgcgtccgct gccggataaa ttccacggct tgcaggatca
    46081 ggaagcgcgc tatcgtcagc gttatctgga tctcatctct aacgatgaat cccgtaacac
    46141 ctttaaagta cgctcgcaga tcctctctgg tattcgccag ttcatggtga accgcggctt
    46201 tatggaagtt gaaacgccga tgatgcaggt gatcccaggc ggtgccgctg cgcgtccgtt
    46261 tatcacccac cataacgcgc tggatctcga catgtacctg cgtatcgcgc cggaactgta
    46321 cctcaagcgt ctggtggtcg gtggcttcga gcgtgtattc gaaatcaacc gtaacttccg
    46381 taacgaaggt atttccgtac gtcataaccc agagttcacc atgatggaac tctacatggc
    46441 ttacgcagat tacaaagatc tgatcgagct gaccgaatcg ctgttccgta ctctggcaca
    46501 ggatattctc ggtaagacgg aagtgaccta cggcgacgtg acgctggact tcggtaaacc
    46561 gttcgaaaaa ctgaccatgc gtgaagcgat caagaaatat cgcccggaaa ccgacatggc
    46621 ggatctggat aacttcgact ctgcgaaagc gattgctgaa tctatcggta tccacgttga
    46681 gaagagctgg ggtctgggcc gtatcgttac cgagatcttc gaagaagtgg cagaagcaca
    46741 tctgattcag ccgaccttca ttactgaata tccggcagaa gtttctccgc tggcgcgtcg
    46801 taacgacatt aacccggaaa tcacagaccg ctttgagttc ttcatcggtg gtcgtgaaat
    46861 cggtaacggc tttagcgagc tgaatgacgc ggaagatcag gcgcaacgct tcctggatca
    46921 ggttgccgcg aaagacgcag gtgacgacga agcgatgttc tacgatgaag attacgttac
    46981 cgcactggaa catggcttac cgccgacagc aggtctgggg attggcatcg accgtatggt
    47041 aatgctgttc accaacagcc ataccatccg cgacgttatt ctgttcccgg cgatgcgtcc
    47101 ggtgaaataa gcattacgtt atgctcacaa ccccggcaaa tgtcggggtt ttttatttaa
    47161 gctgggtaaa tgcagataat cgtattctgg cttcgcgatt tgtcgcctgc atcaccatcc
    47221 acggactgaa cgcccacggc gtggcatcaa taccgcgtaa tacatcagct aaatcacacc
    47281 attgataatc catcacttca tcatcgttga tctgtaacgc actggtggtg cgtgcggcaa
    47341 ataccggaca cacttcattt tccacaatgc ctctcggatc ggtggcgcgg tagcgaaagt
    47401 caggatagat agattcagga ggcgtaattt ccacgccaag ctcataacgg caacggcgga
    47461 tcaccgcgtc ttcgctgctt tctcccagtt gtgggtgccc acaaaccgag ttagtccaca
    47521 cgccaggcca cgcttttttg ctcagtgcgc ggcgggtaac taataattgc cccttggcat
    47581 taaacagcca actggagaac gcgagatgta agagggtgtc tgccgtgtgt gcggcatact
    47641 tttccagcgt acccgtggga actccctgtg cattcaataa aatgacgtgt tccgtttgca
    47701 taatttctca catgtaattc tgatcactca tcattataga taattgaaga gtgaaatgat
    47761 taaccaagag agcgtttatc gacaaccgcc cgttttgagc ggatgccgac aggtaaaaac
    47821 catttaaaaa agtagcatca tatatttgaa gcgactttca atggccattg caacacagtc
    47881 agaaatgaca ggaatagaga gagtaaaaag caacaacaga ctgaatatag cgcgcattaa
    47941 aaaattcata ttattctcca tgctcatttt tcttcagctt gcgcatcaga agccacatac
    48001 gtactgtcct gacttctaaa tctttatcag agacggattc cttcaccagg tcagcatgat
    48061 gctgatagcc attaaagaag acgttcagca caacggcgct aagggttgcg agcataatgc
    48121 cgctatgtag cagcggttgc agtacggccg gtaacttaga aaagaaatcg tgagagagcg
    48181 tcggagtcat gccaacgccg agactgatcg ccacaatata gaggttgtaa cggttggtgg
    48241 tgtagttaca gcgcgacaga attcgaatcc ctgtcgccag taccatgccg aacatcacta
    48301 gaccagcgcc gcccagcaca aattgcggaa tggaggctac cagcaccgcc atttttggca
    48361 ccatgccaaa caggattaat ataattcccg aagaaataca cacccagcgg ctatgaacgc
    48421 gcatcacgct aaccaggcca acgttttgag aaaatgacgt gtgggggaag ctgttaaacg
    48481 tgccgcctat cattgtccct acgccatcga cacgcagccc gcaaataata tcgtgtgaag
    48541 agagtttacg accgactatt tcacccagtg ccaggaacat ccccatcgac tcgataaaca
    48601 cgatgat
//
LOCUS       DAEEGA010000024        48312 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEEGA010000024 DAEEGA010000000
VERSION     DAEEGA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48312
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.024"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            187..480
                     /locus_tag="KLH46_001419"
     CDS             187..480
                     /locus_tag="KLH46_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBE0378155.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="KLH46_001420"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="KLH46_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBE0378156.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="KLH46_001421"
     CDS             complement(1922..2374)
                     /locus_tag="KLH46_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBE0378157.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="KLH46_001422"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="KLH46_001422"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBE0378158.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="KLH46_001423"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="KLH46_001423"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBE0378159.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVMIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="KLH46_001424"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="KLH46_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBE0378160.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="KLH46_001425"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="KLH46_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBE0378161.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="KLH46_001426"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="KLH46_001426"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBE0378162.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="KLH46_001427"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="KLH46_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBE0378163.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="KLH46_001428"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="KLH46_001428"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBE0378164.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="KLH46_001429"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="KLH46_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBE0378165.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="KLH46_001430"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="KLH46_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBE0378166.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="KLH46_001431"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="KLH46_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBE0378167.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="KLH46_001432"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="KLH46_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBE0378168.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="KLH46_001433"
     CDS             complement(15085..15582)
                     /locus_tag="KLH46_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBE0378169.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="KLH46_001434"
     CDS             complement(15579..15941)
                     /locus_tag="KLH46_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBE0378170.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="KLH46_001435"
     CDS             complement(15931..16278)
                     /locus_tag="KLH46_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBE0378171.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VVVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="KLH46_001436"
     CDS             16387..16800
                     /locus_tag="KLH46_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378172.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="KLH46_001437"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="KLH46_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="KLH46_001438"
     CDS             complement(18324..20039)
                     /locus_tag="KLH46_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBE0378173.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIGQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="KLH46_001439"
     CDS             20206..21099
                     /locus_tag="KLH46_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBE0378174.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="KLH46_001440"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="KLH46_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23527)
                     /locus_tag="KLH46_001441"
                     /pseudo
     CDS             complement(21910..23527)
                     /locus_tag="KLH46_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            23822..24496
                     /locus_tag="KLH46_001442"
     CDS             23822..24496
                     /locus_tag="KLH46_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE0378175.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24535..26196)
                     /locus_tag="KLH46_001443"
     CDS             complement(24535..26196)
                     /locus_tag="KLH46_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBE0378176.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="KLH46_001444"
     CDS             complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="KLH46_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBE0378177.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="KLH46_001445"
     CDS             complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="KLH46_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBE0378178.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27339..27411)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27650..27982
                     /locus_tag="KLH46_001446"
     CDS             27650..27982
                     /locus_tag="KLH46_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBE0378179.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27984..29204)
                     /locus_tag="KLH46_001447"
     CDS             complement(27984..29204)
                     /locus_tag="KLH46_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HBE0378180.1"
                     /translation="MKQITFAPRNHLLTNTNTNTWTPDSQWLVFDVRPSGASFTGETI
                     ERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVI
                     AEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLR
                     NVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHAL
                     AFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTF
                     THHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTD
                     IQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLA
                     WMEGGQLWITETDR"
     gene            29305..30405
                     /gene="cbrA"
                     /locus_tag="KLH46_001448"
     CDS             29305..30405
                     /gene="cbrA"
                     /locus_tag="KLH46_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBE0378181.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30366..31703)
                     /locus_tag="KLH46_001449"
     CDS             complement(30366..31703)
                     /locus_tag="KLH46_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0378182.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31778..32926)
                     /gene="dgoD"
                     /locus_tag="KLH46_001450"
                     /pseudo
     CDS             complement(31778..32926)
                     /gene="dgoD"
                     /locus_tag="KLH46_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32923..33540)
                     /gene="dgoA"
                     /locus_tag="KLH46_001451"
     CDS             complement(32923..33540)
                     /gene="dgoA"
                     /locus_tag="KLH46_001451"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBE0378183.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33524..34402)
                     /gene="dgoK"
                     /locus_tag="KLH46_001452"
     CDS             complement(33524..34402)
                     /gene="dgoK"
                     /locus_tag="KLH46_001452"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBE0378184.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     VVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34399..35088)
                     /gene="dgoR"
                     /locus_tag="KLH46_001453"
     CDS             complement(34399..35088)
                     /gene="dgoR"
                     /locus_tag="KLH46_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBE0378185.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35366..36022
                     /locus_tag="KLH46_001454"
     CDS             35366..36022
                     /locus_tag="KLH46_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378186.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36068..36880)
                     /gene="yidA"
                     /locus_tag="KLH46_001455"
     CDS             complement(36068..36880)
                     /gene="yidA"
                     /locus_tag="KLH46_001455"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBE0378187.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36994..37392)
                     /locus_tag="KLH46_001456"
     CDS             complement(36994..37392)
                     /locus_tag="KLH46_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBE0378188.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37632..40046)
                     /gene="gyrB"
                     /locus_tag="KLH46_001457"
     CDS             complement(37632..40046)
                     /gene="gyrB"
                     /locus_tag="KLH46_001457"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBE0378189.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40075..41148)
                     /gene="recF"
                     /locus_tag="KLH46_001458"
     CDS             complement(40075..41148)
                     /gene="recF"
                     /locus_tag="KLH46_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBE0378190.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41148..42248)
                     /gene="dnaN"
                     /locus_tag="KLH46_001459"
     CDS             complement(41148..42248)
                     /gene="dnaN"
                     /locus_tag="KLH46_001459"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBE0378191.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHELGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42253..43656)
                     /gene="dnaA"
                     /locus_tag="KLH46_001460"
     CDS             complement(42253..43656)
                     /gene="dnaA"
                     /locus_tag="KLH46_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBE0378192.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43953..44033
                     /locus_tag="KLH46_001461"
     CDS             43953..44033
                     /locus_tag="KLH46_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378193.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44263..44403
                     /gene="rpmH"
                     /locus_tag="KLH46_001462"
     CDS             44263..44403
                     /gene="rpmH"
                     /locus_tag="KLH46_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBE0378194.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44420..44779
                     /gene="rnpA"
                     /locus_tag="KLH46_001463"
     CDS             44420..44779
                     /gene="rnpA"
                     /locus_tag="KLH46_001463"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBE0378195.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44743..45000
                     /gene="yidD"
                     /locus_tag="KLH46_001464"
     CDS             44743..45000
                     /gene="yidD"
                     /locus_tag="KLH46_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBE0378196.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45003..46649
                     /gene="yidC"
                     /locus_tag="KLH46_001465"
     CDS             45003..46649
                     /gene="yidC"
                     /locus_tag="KLH46_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBE0378197.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46755..48119
                     /gene="mnmE"
                     /locus_tag="KLH46_001466"
     CDS             46755..48119
                     /gene="mnmE"
                     /locus_tag="KLH46_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBE0378198.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11995 a        13060 c        12205 g        11052 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcacgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tgatgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgcagggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaacct ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagtaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 gcggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccacc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttgg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcaaaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgcccaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat tcccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tagtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaac atcgggtttt gcagcaagat ggcaagaccc accacaatcc
    23461 cggcgaaggg aaacagcagc actggcgtga acatcgcgcc gccaaagcgt tgaatttgac
    23521 tgagcatttc cacatcctct tttctcaatt ctgtaagtag gaagcctgat tggttatgcg
    23581 gcagacctgg agcgaggata ggaagatgcg atcggcagaa catgacgacc caatgggatt
    23641 cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct acgttttcgc gaaaaagtag
    23701 acatctgtga gcacaggtac acagcatcga acacatcttt aaaaaaaaga tttttttcaa
    23761 tcgattaagc agaacttgtg ggcgcattac ccgtgcttgc aggcaaaaaa gagagctaga
    23821 gatgatctac aaaagcattg cggagcggtt aagaattcga cttaattccg cggatttcac
    23881 gctaaacagc cttcttcccg gtgaaaaaaa gctggcggaa gagtttgcgg tatcgcggat
    23941 gactatccgt aaagccattg acctgctggt agcgtggggg ctggtggtcc gccgccacgg
    24001 cagcggcact tacctggtgc gcaaagatgt gctgcatcaa accgccagcc tgaccggact
    24061 ggtggaggtg ttaaaacggc agggaaaaac ggtcaccagc caggtgctga tttttgaaat
    24121 catgcctgcg cctccggcca ttgccagcca gttacggatt caaatcaacg agcagatcta
    24181 cttctcccgt cgcgttcgtt ttgtggaagg gaaaccgcta atgctggaag acagctatat
    24241 gccggtaaaa ctgttccgta acctttcgct gcaacatctg gaagggtcga agtttgaata
    24301 tattgaacaa gagtgcggga ttttgattgg cggtaattat gaaagcctga tgccggtgct
    24361 cgccgataga ctgctggcgc ggcaaatgaa ggtagcggaa cacacgccac tgctgcggat
    24421 cacctcgttg tcatatagcg agagcgggga gtttttgaat tattcagtga tgttcagaaa
    24481 tgccagcgaa tactaggtgg agtaccattt acggcgactc cacccggaaa aaagttaacc
    24541 gatactccag aagagcaccg ccagtaattg gggggtgata atgcgcagga acatcactaa
    24601 cggatagaca gtggcgtaag agagcgccgc cgcaccactg gttggatgaa gattattagc
    24661 aaacgccagc gccggaggat cggtcatgga acctgccagc atcccgcaca tggtcaggta
    24721 attcattttg gctaacatcc gcgccagaat gccaacagta atcagcggaa cggcggtgat
    24781 cagggcacca taaccaatcc agcttagccc ttcgccattg accagggtat tcacaaaatc
    24841 cccaccagat ttcagaccaa cgaccgagag gaacagcacg atccccagct cccgcagcgc
    24901 gaggttggca cttggcggca taaaccagta cagcttgccg atactgccga tacgcccgag
    24961 gatcaacgcc ataatcagcg gaccgcccgc cagccccagt ttcaacgcgg ccgggaatcc
    25021 tggcacaaag acgggaatag aacctaacaa tacgccaagc ccgatgccaa taaacactgg
    25081 cagcatctga acctgttgca gtttttgctg cgcattcccc agcacattgg caacggcatc
    25141 aattgcggac ggacgcccca ccaggttgag gatatcgccg aactgcaggc tgatatcgcc
    25201 actggcgacc agttcgaccc cggcacggtt caggcgcgag ataacaacgt catagcgttc
    25261 tttaaagtgc aggtcgcgaa tacgttttcc gagcacgttt tcattggtga ccaccacacg
    25321 ctcgacgcgc aaatcagtgc ctttcgtaga cagcgaggta tcgacctcct gaccaatcac
    25381 cagttgcgca ttatgtaaat ccgctggctg ccccaccaga tgcagcaaat cgcccagttg
    25441 gataatggta tctggcgaag gtacttttag ggtttcttct cgtttcagac gcgagcagat
    25501 aattttgtcg ccgttgagaa tcggtacatc tttaatggct aaatcatgca ggttagggtt
    25561 ctcaacgcga atattgatag tcttgatcaa cgcgccgcca ttggtgcgtg aagactcgtg
    25621 ctgctgagct tctgtctcga cattgacgcg gaaaataacc cgcaacatcc acatggtgaa
    25681 caaaatcccg caaatgccga atggatacgc catcgcgtaa ctcatcccca tctgatcgac
    25741 catttccatt ggtgtaccca ggtcgcgcaa gatctgctgc cctgccccca gcgctggcgt
    25801 attggttacc gcaccggaga aaatccccag cactaccggc agtggaatat caaacagttt
    25861 atgcaggatg gcggtaacca gaccaccgat gatgacgatc agaacagcaa acaggttgag
    25921 gcgtaatccg gagacgcgca atgaggcaaa gaagcccggc cctacctgaa tcccgatggt
    25981 ataaacgaac aggatcaggc caaactcctg aataacatgc agcatatcgc tacttaatgt
    26041 catccccgcc tgagaaacaa aatggccgac gatgatccca ccaaacagca cgccgccaat
    26101 acctaatcct atgccgcgaa atttgacgtt gccgataaac aaaccgacga ctgccaccaa
    26161 agccagaata ctgaccgtta atgctatatc actcatcgtc catttctctt gagataacat
    26221 tttaattgct acggattatg gctgaatcgg tgaagcagat ttgccttata gcgcacatta
    26281 tcacgggaaa gaacgtgccg aaatatctta aacagtcgaa atttaacgca ttgttgaaga
    26341 agaaagacaa aaaaagcccc accagaatgg cggggcaaag agaatagcta gttagctatt
    26401 taacgcggga cgttcgctga tagcgatacg ctgcgctgcg atgggttcag gctcattacg
    26461 aattaaatca atatgcagta aaccgtttac gaaggttgcg ccagagactt ccatattttc
    26521 agccagcgta aaactcaggc taaatggctg attcataagc ccttgatgca gccatttttt
    26581 ctcttctttt ggctgctccg gcgtgccttt tacgctcagg cgcgtacctt ccagttgaat
    26641 ctctaaatct tcctgacgga aacctgccag cgcaagggta atgcggtagt ggttatcgtc
    26701 gcttttctca atgttgtacg gcgggaagct ctggctttca ccggcgtttt gcagtgcgtt
    26761 ggccagtttg tcaaaaccga tccattgacg catcagtggg gataaatcga agttacgcat
    26821 agtcatttct ccttctaaga agcgagtaag tacctgcaaa tccgaagatt cgcatatgct
    26881 ccctgacggc gagcatggag atgtcaggcc gcgccaggcg gccttaggga attagttgat
    26941 ttcgatacgg cgcggttttt tcgcttccgg aatcacgcgt tcgagatcga tatacagcaa
    27001 accatttacc aggttagccc cacgaacatg aatgttctca gctaactgga atttgcgttc
    27061 aaagttgcgt tcagcgatgc cctggtacag ataggtgcgc tctttttgtt cgtcggcgtg
    27121 agcacctttc accaccagca gattatcctg ggcggtaatt tccagttcgc tctcagcaaa
    27181 accagccaca gcgatagcaa tgcggtaatg gttttcgtct accagttcaa cgttatacgg
    27241 agggtagccg ccattactct ggctctggtt gttttctaag tggttaaaca aacggtcaaa
    27301 tccaatagca gaacggtaaa gcggggataa atcaaagtta cgcataatca atagctcctg
    27361 aaatcagcga gaatgtaaga ccttccacaa tggacaggtc aggtagccag aacacccatc
    27421 aggcgcgtcc tcatcgacta cgatgtaaaa tgggtctgga aatgaacttt tcaagcctta
    27481 tcagcggact tttttttgca gtttatggtc tattgcatag actgaggggg cagcacgaaa
    27541 cgttaaagtg cgatagccgc cacagtgcga cgtaatgatg atgttatttt cagcaaggga
    27601 tcgctataac tcatcaggcc aacacccacg atcaacagat gaatttatta tgataagaaa
    27661 tgtgttgtta gcgttcatga tatgcagcgg aatgacatta ctcggaggat gctccagcgt
    27721 gatgtcccat accgggggga aagaaggaac gtatcccggc acccgcgcca gcgccaccat
    27781 gattggcgac gatgaaacca actggggcac taagtcgctg gcgattctcg atatgccgtt
    27841 taccgctgta atggacacgc ttttactgcc gtgggatgtg ttccgcaaag acagctcggt
    27901 gcgctcgcgc gttgaaaaaa gcgaagccaa cgcgcaagcc accaatgcgg ttattccacc
    27961 ggccagaatg cctgataact gatttagcga tcagtttcgg tgatccacag ctggccacct
    28021 tccatccacg ccagccattg accatccggc gagaagacca cggcgtccgc agaaggtgga
    28081 ttggcgtggt tttcggttaa atactcaacc tcgccgcttt gcgcatgggc acaaacaatt
    28141 cgattatcca gcacaaagcc caaccattct cctgacggat gccagttaaa tgcagactga
    28201 atatccgttt tgttatgggt taacttgcgc ggttcgccgc cctgtggcga gataagccac
    28261 agttgcacaa tgccgttatc atcacgcatt aaaaacgcga tttgcgtacc ctgcggatta
    28321 cagcgcaccc agtggcgcgg gacgttgact aaccccggat aagcccgatg atgggtaaag
    28381 gttaaacgtc gctgcacgac gccacgcggt ggcgcgggta gggttgtttc cgttccactt
    28441 aacggcgcat cacctgccga tttccagcta gcttcatctt gcggtaactc aacgataaat
    28501 agctccggca ctttctcgcc ctttggcgaa agtgtgtcgc caataaacgc cagcgcgtga
    28561 tttcctaccc atccttcttc ataagcacga ttgatttcat cactgccagg ctgcggcgtg
    28621 ggcgtggttt tactcaccag cacacaccag tggctaccgc tgtattcacg cggatgttgc
    28681 ttttgtacgt tgaccgggcc aaacggtgca gcaaccccga cgtttcgcaa atccagcgcc
    28741 ggatcgagtt gatgcattac atggtcgtta taggtaaagc tccccctttc accgttcggg
    28801 ctaaagacat gcacatggct gccaccgcgc agcgctcctg gcgtatacgg cgcggtaata
    28861 tccattgcat cgagattgct caccttgccg ccttcagcaa tgactccgcg tcgatgatgg
    28921 aagtcgtaat gccatgtttc atcaggattt tcagggccgt gaataaagac atatttctct
    28981 gacttcggat gaacggtcac cacgccgacg tgtgcgccct gtgacgcgcg atagataacc
    29041 tcgacctcgc ctgtatggat attcacacgc tcaatggtct cgccggtaaa cgacgcccca
    29101 gaaggacgca cgtcaaatac cagccactgg ctgtcgggcg tccaggtatt ggtattggta
    29161 ttggtgagca ggtgattacg gggagcaaag gttatctgtt tcatggcgcg gtcctgatac
    29221 aaaacggttc ccgccatcat acttcacaag gacttcactt tcagacaata gtcttttgcc
    29281 atcgtgatga atgtgagtga aaagatggaa catttcgacg tggcgattat tggcctcggc
    29341 ccggcagggt cggcgttggc acgaaagtta gccggcaaaa tgcaggtgat cgcgctggat
    29401 aaaaagcacc agtgcggtac tgaaggtttc agcaaacctt gcggcggtct gctggcaccg
    29461 gacgcgcagc gatcttttat tcgcgatgga ctgacgcttc ctgtcgatgt gatcgccaat
    29521 ccacagattt tcagcgtcaa aactgtcgac gtcgccgcat cgctcacgcg taactaccag
    29581 cgaagctata tcaatattaa tcgccatgct ttcgacttgt ggatgaaatc gctgatcccc
    29641 gccagcgttg aggtttatca cgatagcctg tgccgaaaaa tctggcgtga ggatgataaa
    29701 tggcatgtca tttttcgtgc agacggctgg gagcaacata ttaccgcccg ctatctggtc
    29761 ggtgccgatg gcgcaaactc gatggtgcgg cgacatctct acccggatca tcaaatccgt
    29821 aaatatgtcg ctatccagca gtggttcgca gagaaacatc cggtgccgtt ctactcctgc
    29881 atctttgata atgcgataac tgactgttac tcatggagta tcagcaaaga tggttatttt
    29941 atctttggcg gtgcctatcc aatgaaagac ggtcagacgc gtttcacgac gctgaaagag
    30001 aaaatgagcg cctttcagtt ccagtttggc aaagcggtga aaagtgagaa atgcacggtg
    30061 ttgtttccct cacgctggca ggattttgtc tgtggtaagg acaacgcctt tctgattggt
    30121 gaagcggcgg gatttatcag cgccagctcg ctggagggga ttagctatgc gctggatagc
    30181 gcagagattc tgcgtgcggt gttcctgaaa cagccggaga agagcaacgc cgcttactgg
    30241 cgcgccacac acaaactgcg tttaaaactc ttcggcaaga tagtaaaaag ccgatgcctg
    30301 accgcaccgg ctttaagaaa gtggattatg cgcagtggtg tggcgcatat tccacagttg
    30361 aaagattatc caacgcgctt cacatcaccc accagcagga tgtaagagag cgcgccaatc
    30421 aacgcgacgg cggagatata aaccagtgca ggcgcgaaac cgtaaccctg cgccaggtac
    30481 cccaccacca gcggaacggt gatgccgccc agaccaccgg cgaagttaaa cacgccgccg
    30541 gttaaaccaa tcaggcgcat cggtgccaga gaagagacca gcgaccaggt aatcgaagca
    30601 aaaccgttgc cgaagaatgc cagcgccatc aggcacataa tcatcatcgg atcgttggtg
    30661 tagttagcgc ccataatgca ggtggagatc agcaagccgc agataatcgg cgttttacgc
    30721 gcaaagccca gtgaaaagcc cttacgtacc agcagatccg cgacccagcc agagagcagg
    30781 acgccgacaa acgccgcgag gaatggcacc gtggtcataa agcctgcttt cagcgccgtg
    30841 attccttttt cctgggttaa atagttcggg aaccaggtta agaaaaacca cagtgcagaa
    30901 gccaccgcaa attgcccaag ataaacgccg atcagtttac gatggaacac cagtttccag
    30961 tctttggctg ttaacggctg acgcgcctct ttcttcaccg gcgcatcacc atccaccaga
    31021 ccgccgccat cacgaatgta atccagttca gctttgctga tacctttggt caggcgcggc
    31081 ggctgataaa ccttaaacca aatcagcgac cagataatgc cgataccacc agtgacaatg
    31141 aacacccagt gccagctcaa catctcctga atccagatca gcagcggagt caaaaacgcc
    31201 agaccgacaa actgaccaga cgtataaaaa ccaacggcgg aagcgcgttc atgttccggg
    31261 aaccagctgg tcaccatccg gttattggtc gggaaagcgg gcgcttcgaa aataccggtt
    31321 atcgcacgca gaccaattaa tgacattaat cccgtggcaa agccttggaa taaagtcacc
    31381 actgaccaac caaatatcgc aataaaataa gtcacgcgag aacctacgcg atctaaaaac
    31441 caaccgccgg ggatctggca tagcgtataa agccaggcga aggccgaaaa tacatagccc
    31501 atttccgctt tcgtaatgcc gaactcttcc tgaatatggg cggaagccac ggccaagtta
    31561 gcgcggtcaa cataacaaat gaccaccgta ataaagatca tcaccagcgt cagataacgc
    31621 cgacgccccg gctttgctgc attaacggga atatccatag cgagctttct ccagattttg
    31681 ggcatagcga agccgctcac catgccctgt aatttacaga gggttatatt tttgtattgc
    31741 tgttttagtg cccgatgagg ggcttacgtg gcaggaatta ccactctgct acgctgttat
    31801 cttcatgacg ccagagcgga ttacgccagt ccggggcatt tttactgaac tcaataactt
    31861 tagcttcgtc gatttccaca cctaagcccg gtttcgttaa cggtttaaag aagccgccaa
    31921 ccatgctgaa gtcttctttg tttttcacaa agtcgagtaa ctccgcgcct ttgttgtaat
    31981 gaatgcccat actttgttac tgaagtaccg cgttatagga aacaaagtcg atatgcaggc
    32041 aagccgccag tgcaatcggt ccgaacggac agtgcggcgc aagggtcaca tcataggctt
    32101 ctgccattcc agcaattttg tagcattcgg taataccgcc tgcatgggag agatccggtt
    32161 gcagaatcga aataccgcct gcctccagca cgcgtttaaa atcgaaacgt gagaacatgc
    32221 gctcacccgc cgccagtgga atatgcgttt gtgccgccaa tttcgggtag tattcggctt
    32281 gttccgccag caccggctct tcaataaaca gcgggcgata cggctccagt tctttaatca
    32341 gcactttcgc cattggcgcg ctgacgcgac cgtggaaatc aagaccaaac tcaatctgat
    32401 tgccaaaagc ttcacgaatt tgtgccacgg tgttgactgc cgcatctacc gcgcgggagt
    32461 tatcaattag ccccagttct tcacaaccgt tcagtttgaa ggtatcgaag ccgatttcgc
    32521 gcagggtttt aatgccgtcg ataacatccg ccggacgatc gccgccgacc caactgtagg
    32581 ctttaatttt gtcgcgaacc aggccgccca tcagttgcca gactggcgca ttcagcactt
    32641 tgcctttgat atcccataac gcctggtcga tcccggcgat ggcgctcatc aggattggac
    32701 cgccacgata aaatccggcg cgatacatca cttgccataa gtcgttgatg cgcgaaggat
    32761 cctgaccaat caaatagtca cccagctcgt gaactgccgc ttccaccgta cgggcgcggc
    32821 cttcaatcac gggctcgccc caaccgacca cgccttcatc ggtttcaatt ttcaggaaca
    32881 tccagcgggg aggtaaacga tacgtggtaa ttttggtgat tttcattgca ctgcctctcg
    32941 atacgcctta acaaatgctg ctgcctgctg cgcggtgcgt tctacggact gcccggcgcg
    33001 ataaagatcg ctgcctaagc ccgccccaac acaacctgcg tctatccact gcgccaggtt
    33061 ttctggcgtc acgccgccaa cggcaaagac tgcgatgtcc ggtggtaata ccgcttttaa
    33121 cgctttgatg tattgcggac caaaagccga tgacggaaat attttcagcg cctgcgcgcc
    33181 cgcttcgagc gcggtaaagg cttcggtcgc cgtcgcgcag ccggggcaaa cggtcatgcc
    33241 gtagcccacc gcgcggcgga tcacttcact atggatattg ggcgtaacga tgagctgaca
    33301 gcccatcctg gcgagcgcat cgacctgttc aggtttcagt accgtacctg cgccaatcaa
    33361 cgccttgtcg ccatacgcat caacgatggc gggaatgctc tgctcccatt gtggggaatt
    33421 cagcgggatt tcaaccgcgt cgaacccggc gtcaatcacc gcgccaacat gcgccagcgc
    33481 ctcgtcgggc gtaatgccgc gcaaaatggc gatcagcggg agtttagttt gccactgcat
    33541 gagcgatgct ccttatacca gcctgaaatg ccgtgtcgcc cgccaccgcc gccacgtcgc
    33601 aacccatcgc ctgaaaggct tgctggtagc gcgcggtcag cgatgttccg gcgacaaggg
    33661 tgatggcgtg ttgatgggtc acatagtcgc gcatactggc aacctctgcg ccaatcaaca
    33721 aaccagagag aaattcgctg acctgttcgc ggggaagtgt tcccagcaca tgcgaggcgc
    33781 gaacttcaaa aagctgcggc aatatggcgg gcgcattaag gccgcgctca aggccagccg
    33841 cgaaggcatc ggcagagttt tcctgcggcg gcaaacctgc gccaatcaat gagtgattta
    33901 acagtaaatg atgtaattca ccggtcatca cggtgcgaaa atcgttgatt tgctggctat
    33961 cggcctgcac ccatttgcaa tgcgtaccgg gcataacata aagagaggaa ggagccagag
    34021 ctcgcgcgcc gatcaattgt gtttcttcgc cgcgcatcac attgtggtta tcgtcatgag
    34081 agacgcataa tccgggaata atccagatat tgtcgccaac agacgttaat tgttcgccaa
    34141 tagacgaaaa acgggcagga acagataaat acggagcaac tttccagccg acattgctgc
    34201 cgaccattcc tgccatcacc actggcgtat tttcttcacg ccagtcggtc gtgacttctg
    34261 ctaacaccac agccggagat tttccgttca ggcgcgtgac gcctgcttct gattgcctgc
    34321 tctccaggca gtggtcgccc tgataaagcc aggcgcgcag attggtcgat ccccagtcaa
    34381 ttgcgatgta gcgagctgtc atgtgatttc ctttaacctt cgtgtcgagc tggcaatcat
    34441 ggtaagcgcc gcctgctctg ccgcatcgcc gtcctgatgc cgtatcgcat cgaacagcgc
    34501 cttatgttcc tggagcgttt gcggcatgtt ggcctcatcg cccatccagg ttcgttcaaa
    34561 aaccgcccgc tgcagcgaac tgatcgcaat gctaagttgc tgtaacaccg ggttatgcac
    34621 cgactgcagc accgcctcgt ggtagcgaat atccgcttcg ttaaacgctt cgcggtcctg
    34681 attgttggca atcatctcgt tcagcgccga ttcaatctgc gccagatcgc tggaggtcgc
    34741 gcgctctgct gcccaacggg caatcgccgg ttccaccaga tttcgcactt cgctcatggc
    34801 actgataagc cgtgggtcgt agtcgttttc cagcacccat tgcagtacgt cagtgtcgag
    34861 gtaattccac tggttacgcg gtgccacaaa cgccccgcga taacgtttca tttcaatcag
    34921 ccgcttcgcc atcagcgaac ggaacacctc acggatgatg ttgcgcgagg ttgcaaactc
    34981 ttcacagagc tccgcctcag ccggaagtgg cgagccaggt acgtatttgc cgtgaacgat
    35041 ctgtttaccc agcgtaatga caatgcgatc ggttttattg agagtcatgg agagtccttg
    35101 tgctctgtat gttcttctct actttacccc gatcgatgca taacgcggca actttgtagt
    35161 accagcgtga tgacgttcgc gtttgccgcg cgcgtaatgt agtacaaact tatattgtta
    35221 tgctacaatt tagatcaaaa aaagaacaat acataaaaaa atgacatgcg tcgggcagaa
    35281 atctgaaaag ggatatcagg cgctaaacag gagggaaaga agagtatgct ttcaacggct
    35341 tagctactcg tttaaaggat taatcatgaa gttgaatttt aagggatttt ttaaggctgc
    35401 cggtttattc ccactggcgc tgatgctttc aggctgtatc tcgtatgctc tggtttccca
    35461 taccgcaaag ggtagttcag gaaagtatca atcgcagtca gacaccatca ctgggctatc
    35521 gcaggcaaaa gatagtaatg gaacaaaagg ctatgttttt gtaggggaat cgctggatta
    35581 ccttatcact gatggtgccg atgacatcgt taagatgctc aatgatccag cacttaaccg
    35641 gcacaatatt caggttgccg atgacgcaag atttgtttta aatgcgggga aaaagaaatt
    35701 taccggcaca atatcgcttt actactactg gaataacgaa gaagaaaagg cactggcaac
    35761 gcattatggt tttgcctgtg gtgttcaaca ctgtaccagg tcactggaaa acctaaaagg
    35821 cacaatccat gagaaaaata aaaacatgga ttactcaaag gtgatggcgt tctaccatcc
    35881 atttaaagtg cgattttatg aatactattc acccagaggc attccggatg gtgtttccgc
    35941 agcattactg ccagtgactg ttacactgga catcattact gcaccgctgc aatttctggt
    36001 tgtatatgca gtaaaccaat aatcagtaag cgggcaaacg cgtttatgct gtttgcccgc
    36061 ttacagatta attcagcaca tacttctcaa tagcaaacgc cacgccatct tcaaggttgg
    36121 attttgtgac aaagttcgcc acttctttca ccgaaggaat agcgttatcc atcgccacac
    36181 cgacgcctgc atattcaatc attgcgatgt cgttttcctg atcgccaatc gccatgattt
    36241 cttccggttt aatacctaac acgtcggcca gtgatttcac acccgtacct ttgttaacgc
    36301 gtttatcgag gatttcgagg aagtacggcg cacttttcag cacggtatat ttctctttca
    36361 cttcctgcgg aatacgcgcg atagcctggt cgaggatggc gggttcatca atcatcatca
    36421 ctttcaggaa ctgggtattg gggtccattt tctccgcttc gcagaacacc agcggaattg
    36481 tggcaacgaa ggattcatgc accgtgtagt agctgatatc gcggttagcg gtgtacagcg
    36541 tggtgcggtc cagggcgtgg aaatgagaac cgacttcgcg agagagtttt tccaggaaac
    36601 gatagtcgtc atagctgaga gcggtttgcg ccacggtgct accatcagcg gccttctgta
    36661 ccagcgcgcc gttataagta atgcagtagt cgcccggctg ttccatatgc agctctttca
    36721 ggtagttgtg cacacctgca tacggacgac ccgtcgttag cacgacattc acgccacggg
    36781 cgcgagctgc ggcaatcgca tttttaacgg cgggtgaaat ggtgtgatcg ggcagcagaa
    36841 gggtgccatc catatcgata gcaatgagtt tgatagccat gagttcccaa ggtagattag
    36901 ttcctgaccc atgctaacgc gattccgctc aaaaatcagt acaacacccg agggaaaagg
    36961 ggatgcaacg cgggttgcat gctccccatt tgcttagcgg aagagtttcc ctttcagcag
    37021 ttccatgcct gcggaaagca gatcgttatt ggcttgtgct gacacttcac cttgcggtga
    37081 gagcgcatca ataatcttcg gcaattgctc tgccagtaaa ctggaagccg tactggtatc
    37141 cacgccaagt ttttgcccga gatcggacac cgcatttgtg ccgagcgccg actccagttg
    37201 ctcgccgcta actgattgat ttctctgttg attactcaac caggttgaga gaatggcccc
    37261 taagccgcca ctttgcagtt tttccagcag cacctgaatg ccaccctgct cctcaaccca
    37321 acttaaaatc gcctgatatt tccccgcatc gcctttcaga aaggcaccga caacttcatc
    37381 aaaaagcccc atgataatca cctgtaaagc gttacgtgtt gacccaaaaa gtatagatct
    37441 gcgggatgat aattgcggat tgcagaaata aaaagggcgg agatgatctc cgcccttttc
    37501 ttatagcttc ttgccggatg cggcgtgaac gccttatccg gcctacaaaa tcatgcaaaa
    37561 tcaatatatt gcaggagtct cgtaggacta ataagcgtag cgcatcaggc acgctcgcat
    37621 ggttaacgcc attaaatatc aatattcgcc gctttcaggg cgttctcttc aataaacgca
    37681 cgacgcggtt ctacggcgtc gcccatcagc gtggtgaaca actggtcagc ggcaatcgca
    37741 tccttaacgg taacgcgcag catacgacgg ctttccgggt ccatggtggt ttcccacagc
    37801 tgttccgggt tcatctcgcc cagaccttta taacgctgga tggagaggcc acgacgggac
    37861 tctttcacca gccagtccag cgcctgctcg aagctggcta ccggctgacg acgctcgcca
    37921 cgttcgataa acgcatcttc ttccagcaag ccacgcagtt tctcacccag cgtgcagata
    37981 cgacgatatt cgccaccggt gataaactcg tgatccagcg gatagtcagt atccacaccg
    38041 tgggtacgca cgcgaacaat cggctcgaac aggttttgtt cggcattggt gtggacatca
    38101 aacttccact ggctgccgtg ctgttctttg tcgttcagtt cgctgaccag cgcgttcacc
    38161 cagcgggtaa cggtctgctc atcagaaagg tcagcttccg tcaacgtcgg ctgatagata
    38221 agctctttca gcattgcttt cggataacga cgctccatgc ggttgatcat tttctgcgtc
    38281 gcgttgtact cagataccag tttctctaac gcttcgccag ccaatgccgg tgcactggcg
    38341 ttggtgtgca gcgttgcgcc gtccagcgcg atagagatct ggtactgatc catcgcttcg
    38401 tcgtctttaa tgtactgttc ctgcttgcct ttcttcactt tgtacagcgg cggctgagcg
    38461 atgtagacgt gaccgcgttc aacgatttcc ggcatctgac gatagaagaa ggtcaacagc
    38521 agcgtacgaa tgtgcgagcc gtcgacgtcc gcatcggtca tgatgatgat gctgtgataa
    38581 cgcagtttgt ccgggttgta ctcgtcacga ccgataccac agccaagcgc ggtgataagc
    38641 gtcgccactt cctgagaaga gagcatctta tcgaagcgcg ctttctcgac gttaaggatt
    38701 ttacccttca gcggcagaat cgcctggttc ttgcggttac gcccctgctt cgcagagccg
    38761 cccgcggagt ccccttccac caggtacagt tcggaaagcg ccggatcgcg ttcctggcag
    38821 tctgccagtt tgcccggcag gccagctaag tcgagcgcac ctttacggcg ggtcatttca
    38881 cgcgcgcgac gcgccgcttc acgggcacgg gcagcatcga taattttgcc aaccacgatt
    38941 ttcgcatcag ttgggttttc cagcaggtat tccgccagca gttcgttcat ctgctgttca
    39001 accgccgatt tcacctcaga agaaaccagt ttgtctttgg tctgggagga gaatttcggg
    39061 tccggcactt ttacggaaac gaccgcaatc aggccttcac gcgcatcgtc accggtggcg
    39121 ctgactttgg cttttttgct gtagccttct ttgtccatgt aggcattcag ggtacgggtc
    39181 atcgccgcac ggaagcctgc caggtgagta ccgccgtcac gctgcggaat gttgttggta
    39241 aagcagtaga tgttttcctg gaagccatcg ttccactgca gcgccacttc aacgccaata
    39301 ccgtcttttt cggtggagaa gtagaagata tttgggtgga tcggcgtttt gttcttgttc
    39361 agatattcaa cgaacgcttt gatgccgcct tcatagtgga agtggtcttc tttgccgtca
    39421 cgcttgtcgc gcagacgaat ggaaacgccg gagttgagga acgacaactc acgcagacgt
    39481 ttcgccagaa tttcatattc gaactcggtc acattggtga aggtttcgag gctgggccag
    39541 aaacgcacca tggtgccggt tttttcagtc tcgccggtaa ccgccagcgg agcctgcggt
    39601 acaccgtgtt cgtagatctg acggtgaatt ttaccctcgc gctgaataac cagctccagt
    39661 ttttgcgaca gggcgtttac taccgaaaca ccaacgccgt gcagaccgcc ggacacttta
    39721 taggagttat cgtcaaattt accgcctgcg tgcagaacgg tcatgatcac ttccgccgcc
    39781 gatacgccct cttccgggtg aataccggtc ggaatgccgc gcccgtcatc ctgtacagag
    39841 acagagttat cggcgtgaat ggtgacgata atttctttac agtgacccgc gagcgcttcg
    39901 tcgatagcgt tatctaccac ctcgaatacc atgtggtgca gaccggtgcc gtcatccgtg
    39961 tcgccgatat acatacccgg gcgcttacgc accgcatcca gccctttcag gactttgata
    40021 ctggaggagt cataagaatt cgacatcaac gtttctcgct catttatact tgggttaatc
    40081 tgttatttta cccttttcca cggtaaacat cttcgaattt tcgtccgaca tgtctataac
    40141 gtgttcagca ctgatcgcgc tgacaaagac ctgtgattgc gtcgctttta agcggctggc
    40201 aagcaacccg cgacgctcat catcaagctc agaggcaaaa tcatctatca ggtagagaca
    40261 ccgccgcccg ctttcacgag tgaggaactc tccttgcgcc agacgtaagg cgcacatcaa
    40321 cagcttaagc tgcccacgcg ataaggtatc ttccaccggc gcaccgtcgg cgcgaatgcg
    40381 taagtccgct ttatgcgggc catgcgcggt gtaggttagc tggcgatcgc gttcaaaatt
    40441 acgttccagc acctcagcgt actctgtctc tttctcccag ccgcgctgga aagagaaagt
    40501 cagagaaaac tcagggagaa attgcttaca ggtatcatcc atatcggccg cgataccggc
    40561 gctatactcc gcgcgccagg tgctgatttg ctccgccagc gggatcagtt ctttatccca
    40621 cgggcgtagc tgttcgtaac gagtcacctg gcgcagcgcc gcattgcgct gcttgagcaa
    40681 tcgcttgaga ttgctccagg cggtgaaaaa tccaggttcg ttgtgaaagc atccccagtc
    40741 gaggaatgct cttctgtatt tggggccgcc gttgagtaaa gtaaaccctt ctggcgttat
    40801 caactgcatc ggcatcaggt gcgccagttc cgcgacttta tgcccgtccg taccgtcaat
    40861 acggactttg ctgtcgccct gtttgtcttt ggttaagcca atcgccgtct cgcgctcttc
    40921 gccctgtaat cgcccgtgga gaacaaatgc ctcctgctca tggcgaatga cgcgaccaat
    40981 ctgcaaactg cgaaacgccc gaccatggcc gagcgtatag atggcttcca gcacgctggt
    41041 tttgccactg ccgttggcac ctaccagaaa gttaaagccg ggagataaag cgagatccgc
    41101 ggtttcaata ttgcggaaat cgcggatcaa caagcgggtg agggacatta cagtctcatt
    41161 ggcatgacaa cataggcagc cgactgtgat gcggcatctt caatctgcac gctggaaacc
    41221 gaatcggtca gcatcatgcg gacgttttcg catttcagcg cgttcagaac atccagcaca
    41281 tagctgacgt tgaagccgat ttccatctcc gcaccgctat aggtaacgtc gaggatctct
    41341 tccgcttctt cctgttccgg gttgttggca gtaattttca gctggttttc gctgacatac
    41401 aggcgcacac cgcggaattt ctcgttagag agaatcgccg cgcgagcaaa cgcctgcttg
    41461 agcagatcgc agcctgcttc cagatgtttg tccgggttct tcggcagaac gcgacggtaa
    41521 tccgggaagc gaccatccac cagtttcgag gtgaaaataa agtcgccaac gtgggcacga
    41581 atgttattgc tgccaatctg cacacgcagc agattgtcgc cgccgtcgag catacgcatc
    41641 agttcaatca cgcctttacg cggtacgatc accgaatggc ttggcaaaga ttgaccaatt
    41701 ggcattgaac agaccgccag acggtggccg tcggttgcca cggtgcgcag ttcttcacct
    41761 tcggtttcaa acagcatacc gtttaagtaa tagcgaacat cctgatgcgc catcgaaaac
    41821 tgggttgctt caatcagacg cttcatcgtc gcctgcggca gggtaaattc gacttcgctc
    41881 tgccagtcat cgaggttcgg gaaatccgcc gctggcaggg tagacagcga aaaacggcta
    41941 cgcccggagc gcaccagcat ccgttcacct tccagctgca cggcaatttc cgcgccttca
    42001 ggcagaccac ggcagatatc aaagaatttg cgcgccggaa cggtcgtcgc tcctagctcg
    42061 tgtggctgaa ccagcgcaac gcgtgccacc atttccatct cgagatcagt accggtcagc
    42121 gacaacgtac cgtcagcaac ctgtaacagc agattaccga gaatcggtag cgtaggacga
    42181 ccacctaacg gaccgctcac ctgttgtagc ggttttaata aatgctcacg ttctacggta
    42241 aatttcatag gtttacgatg acaatgttct gattaaattt gaaaaatctt ctttgatatc
    42301 gtggctctct tcacgcaact gctcgatctt acggcaggca tgaagcaccg tcgtgtggtc
    42361 acgcccacca aacgcatcgc caatctctgg cagactgtgg ttagtcagct ctttcgctag
    42421 cgccatcgcc atctggcgcg gacgcgccac cgagcgggat cgacgcttgg aaaggagatc
    42481 cgcgactttg atcttgtagt actccgccac cgtcttctga atattgtcga tggtgaccag
    42541 tttttcctgc aatgccagca agtcgcgcag cgcctcacgc acgaagtcga tggtgatcgc
    42601 ccgtccggta aagttggcgt tagcaataac gcggttcagc gccccttcca gctcacgtac
    42661 gttagatcgt agacgcttgg cgataaagaa cgccacttca cccggcaaac gaatgtcatt
    42721 ttcgtcggcc tttttcatca ggatcgccac acgggtttcc agctctggcg gttcgatcgc
    42781 cacagtcagt ccccaaccga agcgggattt caaacgatcc tcaacgccgt tgatctcttt
    42841 cggatagcga tccgaggtga gaatgatctg ttgattacct tccagcaggg cgttgaaggt
    42901 gtggaaaaac tcttcctgag atcgttcttt attagcaaaa aactgaatat cgtcgatcag
    42961 cagtgcatct acggaacggt agtagcgttt aaactcttcg atcgcgttgt tttgcagggc
    43021 tttaaccatg tcctgaacaa agcgctcgga gtgcatataa accactttgg cattcggctt
    43081 gcgcgccata atgccgttac ccaccgcatg cagcaggtga gttttaccca gacccgtgcc
    43141 gccataaagg aacaacgggt tataggcacc gccagggtta tccgccacct ggcaagccgc
    43201 cgcgcgcgcc agttggttag atttaccttc aacgaagtta tcaaacgtgt gtttgacgtt
    43261 tacgttagaa cgataggtcg gttctgccgg ggccgggacg ttatcccaac ctgagcgcgt
    43321 agaaggcgca gcacgttgcg gctgcgtttg cgccacctgt gcaggggccg cgacgttgct
    43381 cgtcactgcc gcttgtggcg tttgcgtcac cggtttggtg ccgacttcaa aacgcagctg
    43441 tggggcatcc gctccgcaga aactggttag cagtccattg atattattaa ggtacttgtc
    43501 ccgtacccaa tcgaggacaa aacggtttgg cgcgtacagg gccagcgtgt tatcgctcag
    43561 ttccgcctgc aatgggcgta tccacatact gaattctgtg gctggtaact catcctgcaa
    43621 tcgggcaaga cactgctgcc aaagcgaaag tgacacggcg gactccactc gaacaaaagt
    43681 cgataatgac taaggctgaa acattcatga ttgttgacgt acgtcgaaaa gaccctgctt
    43741 gagggtgacg cacgaaccgc tgtctgcggt tatatgcccg atcaagatcc tgcaaaacga
    43801 tcgggaccgc ggatcatagc ctaaactgcg caagagatct tctgtttctc acagattttc
    43861 cctatttatc cacaggactt tccagaactc gctaagtgta aacgatcctg ccgcgaggcg
    43921 ggcacgattt acgccgcata ttggaaaatt taatgaccat agacaaaaat tggcttaatc
    43981 gatctaataa agatccagga cgatccttgc gctttaccca tcagcccgta taatcctcca
    44041 cccggcgcgc catgctggtt tccactggtg tgaggtcgta cattttccct gcgaaaaggt
    44101 gcggaaaagc gcggtaaata aggaaagaga attgactccg gtgtgtacaa ttattacaat
    44161 ccggcctctt taatcaccca tggcttcggt gtccatcgtt tcatttttcg gcggatatcc
    44221 aataaagcca ttgaatttat tcaagtttag gtagaaatcg ccatgaaacg cacttttcaa
    44281 ccgtctgtac tgaagcgcaa ccgttctcac ggcttccgtg ctcgtatggc tactaaaaat
    44341 ggtcgtcagg ttctggcacg tcgtcgtgct aaaggccgcg ctcgtctgac cgtttctaag
    44401 taataaagct aacccctgag tggttaagct cgcatttccc agggagttac gcttgttaac
    44461 tcccagtcaa ttcacattcg tcttccagca gccacaacgg gctggcacgc cgcaaattac
    44521 cattctcggc cgcctgaatt cgctggggca tccccgtatc ggtcttacag tcgccaagaa
    44581 aaacgttcga cgcgcccatg aacgcaatcg gattaaacgc ctgacgcgtg aaagcttccg
    44641 tctgcgccaa catgaactcc cggctatgga tttcgtggtg gtggcgaaaa aaggggttgc
    44701 cgacctcgat aaccgtgctc tctcggaagc gttggaaaaa ttatggcgcc gccactgtcg
    44761 cctggctcgc gggtcctgat agccctcatt cgggtctatc aacgcctgat tagtccgcta
    44821 ctcgggccgc attgtcgttt cactccaacc tgttcaagct acggaattga ggcattgcgc
    44881 aggtttggag tgataaaagg cagttggttg acggtgaaac gcgtattaaa atgccaccct
    44941 ttacaccctg gtggtgacga tcccgtcccg cccggaccat ttgataccag agaacactaa
    45001 cgatggattc gcaacgcaat cttttagtca tcgctttgct gttcgtgtct ttcatgatct
    45061 ggcaagcctg ggagcaggat aaaaacccgc aacctcaggc ccaacagacc acgcagacaa
    45121 cgaccaccgc agcgggtagc gccgccgacc agggcgtacc ggccagtggc caggggaaac
    45181 tgatctcggt taagaccgac gtgcttgatc tgaccatcaa cacccgtggt ggtgatgttg
    45241 agcaagctct gctgcctgct tacccgaaag agctgaactc tacccagccg ttccagctgt
    45301 tggaaacttc accgcagttt atttatcagg cacagagcgg tctgaccggt cgtgatggcc
    45361 cggataaccc ggctaacggc ccgcgtccgc tgtataacgt tgaaaaagac gcttatgtgc
    45421 tggctgaagg tcaaaacgaa ctgcaggtgc cgatgacgta taccgacgcg gcaggcaaca
    45481 cgtttaccaa aacgtttgtc ctgaaacgtg gtgattacgc tgtcaacgtc aactacaacg
    45541 tgcagaacgc tggcgagaaa ccgctggaaa tctcctcgtt tggtcagttg aagcaatcca
    45601 tcactctgcc accgcatctc gataccggaa gcagcaactt cgcactgcac accttccgtg
    45661 gcgcggcgta ctccacgcct gacgagaagt atgagaaata caagttcgat accattgccg
    45721 ataacgaaaa cctgaacatc tcttcgaaag gtggttgggt ggcgatgctg caacagtatt
    45781 tcgcgacggc gtggatcccg cataacgacg gtaccaacaa cttctatacc gctaatctgg
    45841 gtaacggcat cgtcgctatc ggctataaat ctcagccggt actggttcag cctggtcaga
    45901 ctggcgcgat gaacagcacc ctgtgggttg gcccggaaat ccaggacaaa atggcagctg
    45961 ttgctccgca cctggatctg accgttgatt acggttggtt gtggttcatc tctcagccgc
    46021 tgttcaaact gctgaaatgg atccatagct ttgtgggtaa ctggggcttc tccattatca
    46081 tcatcacctt tatcgttcgt ggcatcatgt acccgctgac caaagcgcag tacacctcca
    46141 tggcgaagat gcgtatgctg cagccgaaga ttcaggcaat gcgtgagcgt ctgggcgatg
    46201 acaaacagcg tatcagtcag gaaatgatgg cgctgtacaa agctgagaag gttaacccgc
    46261 tgggcggctg cttcccgctg ctgatccaga tgccaatctt cctggcgttg tactacatgc
    46321 tgatgggttc cgttgaactg cgtcaggcac cgtttgcact gtggatccac gacctgtcgg
    46381 cacaggaccc gtactacatc ctgccgatcc tgatgggcgt aacgatgttc ttcattcaga
    46441 agatgtcgcc gaccactgtg accgacccga tgcagcagaa gatcatgacc tttatgccgg
    46501 tcatcttcac cgtgttcttc ctgtggttcc cgtcaggtct ggtactgtac tatatcgtca
    46561 gcaacctggt aaccattatt cagcagcagc tgatttaccg tggtctggaa aaacgtggcc
    46621 tgcatagccg cgagaagaaa aaatcctgat tcggtgagtt ttcgctaaaa taagggcggt
    46681 cagttgaccg ccttttttct tttcgtaggg cggataagca ccgcgcatcc gccacacaaa
    46741 gcaacaggaa catcatgagc gataatgaca ctatcgtagc ccaggccacg cctccgggac
    46801 gtggcggcgt tggcatcctg cgcatctccg gcttcaaagc ccgtgaagtt gccgaaaccg
    46861 tgctgggtaa actgcctaag ccgcgctacg ccgattatct tccgtttaaa gacgccgacg
    46921 gcagcgtgct cgatcagggg attgcgctat ggttccctgg cccgaactcg ttcaccggcg
    46981 aagatgtgct ggaactgcaa ggtcatggcg gtccggtgat cctcgacctg ctgttaaaac
    47041 gcattctgac cattcccggc ctgcggattg ctcgccctgg tgagttttcc gaacgcgcgt
    47101 ttcttaacga taaacttgac ttagcccagg ccgaggcgat tgccgatctt atcgacgcca
    47161 gttcggaaca ggcggcccgt tcggcactta actcgctgca aggcgcattc tccgcacggg
    47221 ttaatcatct ggtagaagcc ctcacccact tgcgcattta cgtcgaagcg gcaattgatt
    47281 tccctgatga agagatcgat ttcctctccg acggcaaaat tgaagcccag cttaatgacg
    47341 tgatggccga tctcgatgca gtgcgtgctg aagcacgtca gggtagtttg ttgcgcgaag
    47401 ggatgaaagt agtgattgcc ggacgtccta atgccggtaa atcgagcctg ttaaacgcgc
    47461 tggcgggccg tgaagcggca atcgtaaccg atatcgccgg aaccacgcgt gacgtgctgc
    47521 gtgagcatat ccacattgac ggaatgccgc tgcatatcat cgacactgcc gggctacgtg
    47581 aggccagtga cgaagtggaa cgtattggta tcgagcgcgc gtggcaggaa attgaacagg
    47641 ccgaccgcgt gctgtttatg gtcgatggca ccacgacaga cgccgttgat ccggcagaga
    47701 tctggccgga atttatcgcc cgtctgcccg cgaaactgcc gatcaccgtg gtacgcaata
    47761 aagccgatat caccggcgaa acgctgggaa tgagtgaagt gaacggtcac gcgttaattc
    47821 gtctctcggc aaggactggt gaaggcgtgg acgtgctgcg taaccatctc aaacagagca
    47881 tgggctttga caccaatatg gaaggcggct tcctggcgcg ccgtcgccac ttacaggcgc
    47941 tggaacaggc agcggagcat ttgcagcaag gtaaagcgca actgttggga gcctgggcag
    48001 gtgaactgct ggcggaagag ttgcgcctgg cacagcagaa cttaagcgaa atcaccgggg
    48061 aatttacttc agacgacctg ctggggcgga ttttctccag cttctgtatt ggtaagtaac
    48121 cgcgcttacg aagccgtatt ctgactgtca gatgcggctt cgcttcattg ttagcactcc
    48181 tgttatgcct caaccctttt ttaaacatta aaattcttac gtaatttata atctttaaaa
    48241 aaagcattta atactgctcc ccgaacgatt gtgattcgat tcacatttaa acaatttcag
    48301 aatagacaaa aa
//
LOCUS       DAEEGA010000025        47696 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEEGA010000025 DAEEGA010000000
VERSION     DAEEGA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47696
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.025"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="KLH46_001467"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="KLH46_001467"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBE0378199.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="KLH46_001468"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="KLH46_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBE0378200.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="KLH46_001469"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="KLH46_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBE0378201.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="KLH46_001470"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="KLH46_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBE0378202.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="KLH46_001471"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="KLH46_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBE0378203.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="KLH46_001472"
     CDS             5377..5835
                     /locus_tag="KLH46_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBE0378204.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="KLH46_001473"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="KLH46_001473"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBE0378205.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="KLH46_001474"
     tRNA            6887..6971
                     /locus_tag="KLH46_001474"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="KLH46_001475"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="KLH46_001475"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="KLH46_001476"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="KLH46_001476"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBE0378206.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="KLH46_001477"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="KLH46_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBE0378207.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="KLH46_001478"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="KLH46_001478"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBE0378208.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="KLH46_001479"
     CDS             complement(10252..13995)
                     /locus_tag="KLH46_001479"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBE0378209.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDRQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="KLH46_001480"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="KLH46_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBE0378210.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="KLH46_001481"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="KLH46_001481"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBE0378211.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="KLH46_001482"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="KLH46_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBE0378212.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="KLH46_001483"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="KLH46_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBE0378213.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="KLH46_001484"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="KLH46_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBE0378214.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="KLH46_001485"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="KLH46_001485"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBE0378215.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="KLH46_001486"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="KLH46_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBE0378216.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="KLH46_001487"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="KLH46_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBE0378217.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="KLH46_001488"
     CDS             23392..23499
                     /locus_tag="KLH46_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE0378218.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="KLH46_001489"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="KLH46_001489"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBE0378219.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="KLH46_001490"
     CDS             complement(24538..25347)
                     /locus_tag="KLH46_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBE0378220.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="KLH46_001491"
     CDS             complement(25351..25743)
                     /locus_tag="KLH46_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBE0378221.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="KLH46_001492"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="KLH46_001492"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBE0378222.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="KLH46_001493"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="KLH46_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBE0378223.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="KLH46_001494"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="KLH46_001494"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBE0378224.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="KLH46_001495"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="KLH46_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBE0378225.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="KLH46_001496"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="KLH46_001496"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBE0378226.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELPEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="KLH46_001497"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="KLH46_001497"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBE0378227.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="KLH46_001498"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="KLH46_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBE0378228.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="KLH46_001499"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="KLH46_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBE0378229.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="KLH46_001500"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="KLH46_001500"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBE0378230.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="KLH46_001501"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="KLH46_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBE0378231.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="KLH46_001502"
     CDS             36790..39657
                     /locus_tag="KLH46_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE0378232.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="KLH46_001503"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="KLH46_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBE0378233.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTVDLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="KLH46_001504"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="KLH46_001504"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBE0378234.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLEACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="KLH46_001505"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="KLH46_001505"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBE0378235.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="KLH46_001506"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="KLH46_001506"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBE0378236.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="KLH46_001507"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="KLH46_001507"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBE0378237.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="KLH46_001508"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="KLH46_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11974 a        12291 c        11679 g        11752 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgccgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggca cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc gcggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcta
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccac ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccaa gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aagcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccggtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct gtagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggt ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgatca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgcggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatcgt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agactcttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacactgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccgcgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttgacg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tgccggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgcttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggagg
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ttggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta cgacaactta gggaataact cgatataaac tcacctttgt
    36601 aagggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtcca cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgcctgttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 cagtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggaggcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtcgg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgccaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccga acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacagtta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaagtcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tggtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatga
//
LOCUS       DAEEGA010000026        47295 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEEGA010000026 DAEEGA010000000
VERSION     DAEEGA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47295
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.026"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="KLH46_001509"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="KLH46_001509"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBE0378238.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="KLH46_001510"
     CDS             complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="KLH46_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBE0378239.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="KLH46_001511"
     CDS             complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="KLH46_001511"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBE0378240.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="KLH46_001512"
     CDS             complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="KLH46_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBE0378241.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="KLH46_001513"
     CDS             complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="KLH46_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBE0378242.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="KLH46_001514"
     CDS             complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="KLH46_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBE0378243.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="KLH46_001515"
     CDS             complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="KLH46_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBE0378244.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="KLH46_001516"
     CDS             complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="KLH46_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBE0378245.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="KLH46_001517"
     CDS             complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="KLH46_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBE0378246.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="KLH46_001518"
     CDS             complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="KLH46_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBE0378247.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="KLH46_001519"
     CDS             complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="KLH46_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBE0378248.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="KLH46_001520"
     CDS             complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="KLH46_001520"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBE0378249.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10285..11088
                     /gene="suhB"
                     /locus_tag="KLH46_001521"
     CDS             10285..11088
                     /gene="suhB"
                     /locus_tag="KLH46_001521"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBE0378250.1"
                     /translation="MHPMLNIAVRAARKAGNLVAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11233..12087
                     /locus_tag="KLH46_001522"
     CDS             11233..12087
                     /locus_tag="KLH46_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE0378251.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12278..13558
                     /gene="csiE"
                     /locus_tag="KLH46_001523"
     CDS             12278..13558
                     /gene="csiE"
                     /locus_tag="KLH46_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBE0378252.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13550..14689)
                     /locus_tag="KLH46_001524"
     CDS             complement(13550..14689)
                     /locus_tag="KLH46_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBE0378253.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="KLH46_001525"
     CDS             complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="KLH46_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBE0378254.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15875..17236
                     /locus_tag="KLH46_001526"
     CDS             15875..17236
                     /locus_tag="KLH46_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBE0378255.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17233..17751
                     /locus_tag="KLH46_001527"
     CDS             17233..17751
                     /locus_tag="KLH46_001527"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBE0378256.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17751..18071
                     /locus_tag="KLH46_001528"
     CDS             17751..18071
                     /locus_tag="KLH46_001528"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBE0378257.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18068..18880
                     /gene="hcaB"
                     /locus_tag="KLH46_001529"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18068..18880
                     /gene="hcaB"
                     /locus_tag="KLH46_001529"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBE0378258.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18890..20092
                     /locus_tag="KLH46_001530"
     CDS             18890..20092
                     /locus_tag="KLH46_001530"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HBE0378259.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            20189..20611
                     /locus_tag="KLH46_001531"
     CDS             20189..20611
                     /locus_tag="KLH46_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBE0378260.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20659..21531)
                     /locus_tag="KLH46_001532"
                     /pseudo
     CDS             complement(20659..21531)
                     /locus_tag="KLH46_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
     gene            complement(21543..22637)
                     /locus_tag="KLH46_001533"
     CDS             complement(21543..22637)
                     /locus_tag="KLH46_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBE0378261.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22670..23668)
                     /locus_tag="KLH46_001534"
     CDS             complement(22670..23668)
                     /locus_tag="KLH46_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0378262.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23693..25204)
                     /locus_tag="KLH46_001535"
     CDS             complement(23693..25204)
                     /locus_tag="KLH46_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBE0378263.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25227..26210)
                     /locus_tag="KLH46_001536"
     CDS             complement(25227..26210)
                     /locus_tag="KLH46_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBE0378264.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(26307..29408)
                     /locus_tag="KLH46_001537"
     CDS             complement(26307..29408)
                     /locus_tag="KLH46_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBE0378265.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFYPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29415..29588)
                     /locus_tag="KLH46_001538"
     CDS             complement(29415..29588)
                     /locus_tag="KLH46_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378266.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29706..30899
                     /locus_tag="KLH46_001539"
     CDS             29706..30899
                     /locus_tag="KLH46_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBE0378267.1"
                     /translation="MRACINNQQILHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="KLH46_001540"
     CDS             complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="KLH46_001540"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBE0378268.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32677..33867
                     /gene="hmpA"
                     /locus_tag="KLH46_001541"
     CDS             32677..33867
                     /gene="hmpA"
                     /locus_tag="KLH46_001541"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBE0378269.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="KLH46_001542"
     CDS             complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="KLH46_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBE0378270.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="KLH46_001543"
     CDS             complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="KLH46_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBE0378271.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="KLH46_001544"
     CDS             complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="KLH46_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBE0378272.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="KLH46_001545"
     CDS             complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="KLH46_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBE0378273.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLVKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38516..42403)
                     /gene="purL"
                     /locus_tag="KLH46_001546"
                     /gene_synonym="purI"
     CDS             complement(38516..42403)
                     /gene="purL"
                     /locus_tag="KLH46_001546"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBE0378274.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLSLGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42662..44218
                     /gene="mltF"
                     /locus_tag="KLH46_001547"
     CDS             42662..44218
                     /gene="mltF"
                     /locus_tag="KLH46_001547"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBE0378275.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44215..44751)
                     /gene="tadA"
                     /locus_tag="KLH46_001548"
     CDS             complement(44215..44751)
                     /gene="tadA"
                     /locus_tag="KLH46_001548"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBE0378276.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAVGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="KLH46_001549"
     CDS             complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="KLH46_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBE0378277.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45620..46468
                     /locus_tag="KLH46_001550"
     CDS             45620..46468
                     /locus_tag="KLH46_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBE0378278.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46807..47295)
                     /locus_tag="KLH46_001551"
                     /pseudo
     CDS             complement(46807..47295)
                     /locus_tag="KLH46_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11282 a        12955 c        11887 g        11171 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtattttaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta cacatgagca acgcaggtgc ttcacagcaa
      601 aacaatactc accagtaact ctctttttgt caagcaaaag agagtaatta ttgtttattt
      661 agcgtattat cgacaccggc cctttccgcc gtgttcggta ataaaataac ctggcttatt
      721 agtccgaatt cagacaaata taaacaaatc ctgctcaaaa ttaaaaattc taaccggtaa
      781 aagatattac ttaaacatgt aaatttactt tcctttaaaa aacaaaaaac cgccaaaatc
      841 aggcggttat ttgttgctgg tccggttcgc ggcctttcca gcaggttgta ttaccgtagt
      901 aatgcaagcg cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa
      961 agagagttat tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca
     1021 agcgggcgct ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg
     1081 cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc
     1141 acaatgaaga tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa
     1201 gtaaataata accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc
     1261 agcaagtaag cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttttgtt
     1321 taaagtcacg caaaaaacca ccccagcgac gttcatagaa tggcgcaata tgttcggtaa
     1381 taaagtggct aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc
     1441 caggtaatgt atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat
     1501 cggcttcaat gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa
     1561 taaaagcacg cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt
     1621 gtgctggcgg ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct
     1681 ggctgctcag cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt
     1741 ctttaccggt cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca
     1801 atgtgcgaac gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt
     1861 ctaacgaggt gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat
     1921 caagcgcact atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg
     1981 tggattccag tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt
     2041 ccagcaggtc ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt
     2101 tcggggcgca aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt
     2161 tgaatttgca gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt
     2221 acgccgttaa cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact
     2281 gttcaaccgg cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat
     2341 agttctcaac aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg
     2401 cgcttccggt attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg
     2461 ccagcggcag acgccagaat ggttcgttct cttgtgaggc actcgccagt aaacgaccgg
     2521 caagcgcatc gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg
     2581 ccccggtgag ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca
     2641 gaccatcggc aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac
     2701 cgttgcgata ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg
     2761 cacagcagag gaacagcttc acgcgcttgt tcagtccgcg cataatggca aatgccagcg
     2821 ccccggtaac cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct
     2881 gtttgatgct gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca
     2941 ccggtgcttc tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt
     3001 ctgaaccgcg cccgacagtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt
     3061 tggtgatccg ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct
     3121 gtgccagttg cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt
     3181 cgatgatcat caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact
     3241 ccactttacg cgtgcctttc gggcctttat aaccttgcca gaatgcccag cagcgatccg
     3301 catcccagcc ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcggg
     3361 ccgcacgctg gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat
     3421 tattaatgct gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg
     3481 taatcttcat cgcttctgtc attttagtta tccttattcg tttaacagca aaacgggcta
     3541 cccccaggca gcccgttcat tcactcggct tcgtccagcc agactaacaa aatcgcttcg
     3601 aggattttct cgttggatgc ctgcgggtcg tcgtcgaaat cttccagatc gcaaatccac
     3661 tgatgcatat cggtgaatcg aaccgttttc ggatcaagat cgggatacgc atcgtacagt
     3721 gcttcgccaa tttcgcggct atcggtccac ttaagtccca tactaacctc tgttaatgct
     3781 cacgcgcatg gttgatagtg taacgcggga tttcgaccac taaatcttcg tcggtgacgc
     3841 gcgcctggca gcttaaacgg ctttccggct ccagtcccca ggctttgtcc agcatgtcgt
     3901 cttcctgctc tgagctttcc ggcagtgagt caaaaccttc acgaacgatg cagtggcagg
     3961 tggtgcaagc acaggatttt tcacaggcgt gttcaatctc aataccgtta cgcagcgctg
     4021 cgtcgagaat ggtttcaccg ctattagctt ccagaacagc gccatcaggg cagaggtcct
     4081 gatgaggcaa aataacaatc tttggcatat taaacctcgt ccacggaatg gcctttcagc
     4141 gcacgacgaa ccgactggtc catgcggcga gcggcgaaat cctgggtttg tttgtctacg
     4201 tttttaatcg cttgttcgat ggcgtcaaca tcatcgccct gcgccacttc actcaggtga
     4261 gcggcagcat tgtcaatgac ctgacgctct gcggcgctta acagcgcggc atcagcagcc
     4321 agcgcgccgt gcagactttc cagcacacgc gccgcttcta ctttttgttc tgccagcata
     4381 cgggctttta cgtcctgctc ggcatagctc attgagtctt tgatcatcga agcgatttcg
     4441 ctatcggtca gaccgtaaga cggtttgacc tgaatagacg cctcaacgcc ggtggatttc
     4501 tccatcgccg tcacgctcaa aagaccgtcg gcatcgacct ggaacgtcac gcgaatatgc
     4561 gcaccgccag ccggtagcgc cggaatacca cgcagcgcaa aacgcgccag tgagcggcag
     4621 tcctgcacca gttcgcgctc accctgcatt acatggatag acatcgccgt ctgaccatct
     4681 ttaaaggtgg tgaaatcctg agcgcgggcc accggaatag tggtattacg cggaatcact
     4741 ttctccacca ggccgcccat cgtttcgagg cccagcgaca gtgggatcac atcaagcagc
     4801 agcatttcgc tgtctggctt gttacccacc agaatatccg cctgaatcgc cgcgccaata
     4861 gcgacgactt tatccgggtc gatggaagtc agcggtggac gaccgaaaaa ttcgcctacc
     4921 cgttcacgca ccagcggcac gcgagtagaa ccgcccacca tcaccacttc cagcacttcg
     4981 tcagcttcta cacccgcgtc tttcagcgcg cgacgacaag ccagtaaggt tcgttttacc
     5041 agtggcgcga tcagttcatt gaattgttca cggctgattt cgccctgcca gcccgcaacg
     5101 ttaacggtca cggagtccgc atcgctcagc gcgattttgg ctgcaatggt ggcatccagc
     5161 agttcacgct gaacgcggtt atcgctacga tcaggaatgt ccgcctgctc gcgaatgtaa
     5221 tccgccagca gatggtcgaa atcatcgccg ccgagcgcgg aatcaccgcc ggttgccagc
     5281 acttcaaaca cgccgcgact taagcgcaga atggaaatat caaacgtccc gccaccgagg
     5341 tcataaacag cgatcacgcc ttcctgaccg gaatccagcc cgtaggcgat agccgcagca
     5401 gtcggttcgt taagtaagcg cagaacatgc aaccccgcca gacgcgccgc gtctttggtg
     5461 ccctgacgct gggcatcgtc aaagtacgcc ggaacggtga taactacacc atccagctcg
     5521 cctgccaggg cttcagttgc ccgcgccgcc agtgctttga ggatgtccgc agaaacgcgc
     5581 accgggttca gtagccccgc cgccgtttca atcatcggca ggccgttttc gctggcctgg
     5641 aattgataag gcagatgcgg atagcgttgc tggatatcag ccagcgagcg ccccatcagg
     5701 cgtttaacag aactaatggt gttggcggtg tcaagcgctg cgttagtacg cgcgtcataa
     5761 cccaccgaat gcccttgctg ttgatagtga acaacagatg gcagcaggtg acggccttca
     5821 tggtcggcta acgtttcggc ctgaccgctg cgcactgtcg ccaccagcga gttggttgtg
     5881 cccaggtcaa taccagccgc cagacgacgc tgatgcggcg cagcactcaa accaggttca
     5941 ctaatttgta ataaggccat gtttagcttc cagaaattaa aaatcgagca gtttttcttc
     6001 gagttgttcg gcactgctcc gcagtttatc gagaaaacgc agcttacgca ccgtatccgc
     6061 cgccgcgtcc cacgcctcgt tgtctaactg ttcaaccatc aactgatggc gggtatcaaa
     6121 catctttttc acacgtttga taaagctttc cagccgcgct tcatctttcg cctgttcgat
     6181 ctcgtccagc tcttcgcgca gctccaactg ttccatcagg aacgcggtgt cgcgcacagt
     6241 atgctgctcg ctggcgagat caaaaccgtg taaagaaagc aaatattccg cgcgcattaa
     6301 cggatgacgc agcgtttgcc aggcctggtt aatggttgca gattgctgta cggcggcgag
     6361 ttgttccgcc aggcttccgc tggcgaattt atcaggatga tactgacgtt gtagatcctg
     6421 aaaacgcagg ctcagtgcct gggtatcgag ttgataacgg gcaggcaagc caaagagggt
     6481 gaagtaatcc ataacattct cagggctgcg atccgccgcg ataacggcga atcaggtaaa
     6541 acaaacccca cgcgcaggcg accacggtgg ggttatcggt atgcgcatca aacgtggaag
     6601 ctttcgccgc aaccacactc atctttaacg ttcgggttgg tgaatttaaa cccttcattc
     6661 aggccttctt ttacgaagtc cagctgcgta ccgtccagaa attgcaggct tttgccatcg
     6721 accacgactt tcacgccttt gtcttcaaac acgatgtctt ccggcgtcgg ttcgtcaaca
     6781 aattccagta cataagccat acctgaacat ccggaggttc tcacgcccag acgcaggcca
     6841 aaccctttgc cgcggttagc cagaaaggta tttactcgcg ctgctgcact gtcgctcagt
     6901 gtaatcgaca taaccaaacc tcaactctta ttttgcttca cgtttgcttt tatagtccgc
     6961 aatggcggct ttgatcgcgt cttctgccag aatagaacag tgaattttca ccggcggcag
     7021 ttcaagttct tcagcaatat cggtgttttt gatcgcctgc gcttcgtcga gagacttccc
     7081 tttcacccat tcggtgacca gggagctgga agcgatagcg gaaccgcagc cgtaagtttt
     7141 aaaacgcgcg tcttcaatga taccttcatc gttgacttta atctgcaact tcattacgtc
     7201 gccacaggcc ggtgcaccca ccatgccgct gccgacgttc tcgtcgttgt tatcaaagga
     7261 acccacgtta cgcggattct cgtaatggtc gataactttt tcgctgtaag ccattataaa
     7321 ttctcctgat tccgataccg attaatgatg agcccattcg atgctgttca gatccacgcc
     7381 ctgcttgtac atttcccaca gcggagaaag gtcacgcaga cgaccgatgg atttacgaac
     7441 taactcgatg gtgtagtcga tctcttcttc agtagtaaaa cgacctaaag agaaacggat
     7501 agagctatgt gccagctcgt cgttcagccc cagcgcgcgc agcacgtagg acggttcgag
     7561 gcttgctgac gtacaggcgg aacctgaaga aactgcgagg tctttcagcg ccataatcag
     7621 cgactcacct tcaacgtagt tgaagctgac gttgagaatg ttcggcgcac cgtgttccag
     7681 gtcaccgttc aggtaaactt cttcgatatc tttgatgccg ttccacagac ggttacgcag
     7741 gccgcgcaga cgttccatct cggtcgccat ctcttctttt gcgatgcgat aggcttcgcc
     7801 cattccgacg atctggtgaa caggcagagt gccggaacgc ataccgcgct cgtgaccgcc
     7861 gccgtgcatt tgcgcttcga tgcgtacacg cggtttacga cgcacataca gcgcaccgat
     7921 acctttcggg ccatagattt tgtgaccgga gaaagacatc aggtcaactt tcaactggct
     7981 caggtcgatt ggcagtttgc ccacgctctg ggttgcatca acgtgataga taatgccacg
     8041 agcacggcac atttcgccga tagccgcgat atcctgcacc acgccgattt cgttatttac
     8101 gtgcatgatg gaaacaagga tggtgtcgtc acgcatcgcc gcttcgagtt ctttcaggtc
     8161 gataataccg ttacgctgcg gtgccaggta ggtgacttcg aaaccttcgc gctccagctg
     8221 acggcaagta tccagtaccg ctttgtgttc ggttttgctg gtgatgatgt gcttgccttt
     8281 tttctgataa aagttggctg cacctttgat cgccaggttg tcagattcgg ttgcaccaga
     8341 ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg gcaatctgat tacgggcgat
     8401 atctaccgct tcttcagcct gccagccgaa acggtgagaa cgagaggccg ggttaccaaa
     8461 ggttccgtcc atcgtcatga actgcatcat tttctcggca acacgcgggt ccaccggcgt
     8521 ggttgcggag tagtcgagat aaatcggtaa tttcattgct ctataaactc cgtacatcac
     8581 tcaatgcaag gaatcaggct accggctgga tgtacgaccg tgtttacgga gtatttagca
     8641 ctccggcctg attctgaatt ctttttatta agcgcgtaac ttaacgtcga tcgcgtcttg
     8701 tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca gacacatcca gcacttcctg
     8761 gttattaacc agttcgccta aagtaatgtt gttgagaaaa ccggtgagac ggtcgctcaa
     8821 atcacgccac agcgcgtggg tcaggcattt atcgccgccc tggcagccgc ctttaccctg
     8881 acaacgggtg gcatctacag attcgtcaac ggcgctaatc acttcgccaa cggcgatgct
     8941 gctggcatct ttgcctaaca gataaccacc gcctggtcca cgtacgctgg aaaccagacc
     9001 atttttacgc agacgggaaa acagttgttc cagataagaa agggaaattc cctgacgttc
     9061 ggaaatatca gccaacggta ccgggcccgc ttcagagttg agcgcaacgt caagcattgc
     9121 ggtcacggca tagcgccctt tagatgtcag tctcatgtct tacttcacct caaactcgcc
     9181 cctgcccggg gttttttatt gtaaaagtgg gggtattgca tagcagggtc aagtctgaca
     9241 ttcccgagta aattggtcaa ctatttactt gactgattta gtcgggtatt taaccttcag
     9301 tgccattttt ttatcgtggc gtttgtacct gttgtcggat gcggtgtgaa caccttatcc
     9361 gacctacgat tctgtacatt gtaggcctga taagacgcgt taagcgtcgc atcaggcaac
     9421 cactgttatt cggccttgtt acctttattc tgctgctcaa tagaagccag aatcccgcgc
     9481 aggatattca attcctggct ttccgggcgc gcacgggtaa acagacggcg caatttattc
     9541 atcacctgcc ccggatggtt ttcacggata aaaccggttg ccagcagcgt ttgttccaga
     9601 tgaccataaa aacgctccag atcatcgacc agcggatacg gcgtctcttc atgctcgacc
     9661 tgctcgccat tttcctgagt tgccagccag gccatacgca cttcataggc aataacctga
     9721 accgccattg ccaggttcag cgaactgtat tccgggtttg ccgcaatcgc gacatgataa
     9781 tggcatttct gcaactcttc attggtcaag ccgacgcgct cacgaccaaa caccagcgcc
     9841 accggggtat ttgccgcttc agcgacgctt ttcagaccgc attcgcgcgg gtcgagcatc
     9901 ggccacggca gcgtgcggga gcgtgcgctg gtgcccacca ccaggctaca accggctaac
     9961 gcttcgtcga gcgtatcgac gatgtgagca ttaccaatca catcgctggc ccctgcagcc
    10021 agggcaatcg cctgggagtc gggtttcacc agtggattaa ccagccacag attggttaat
    10081 cccattgttt tcatggcacg ggcaacagaa cccatattgc cggtgtgtga cgtctccacc
    10141 agcacaattc gaatattttg cagcattgtc tttcttcatc taaagattat tcacgcatct
    10201 tatcataaaa cgaagacaga tgccgatctc gctgctatac tctgcgccgt tttcccgttc
    10261 tttaacatcc agtgagagag accgatgcat ccgatgctga acatcgccgt gcgcgcagcg
    10321 cgcaaggcgg gtaatttagt tgccaaaaac tatgaaaccc cggacgctgt agaagcgagc
    10381 cagaaaggca gtaacgattt cgtgaccaac gtagataaag ctgccgaagc ggtgattatc
    10441 gacacgattc gtaaatctta cccacagcac accatcatca ccgaagaaag cggtgaactt
    10501 gaaggtactg atcaggatgt tcaatgggtt atcgatccac tggatggtac taccaacttt
    10561 atcaaacgtc tgccgcactt cgcggtatct atcgccgttc gtatcaaagg ccgcaccgaa
    10621 gttgctgtgg tatacgatcc tatgcgtaac gaactgttca ccgccactcg cggtcaaggc
    10681 gcacagctga acggctaccg tctgcgcggt agcaccgctc gcgatctcga cggtaccatt
    10741 ctggcgaccg gcttcccgtt caaagcaaaa cagtacgcca ctacctacat caacatcgtc
    10801 ggcaagctgt tcaacgaatg tgcagacttc cgtcgtaccg gttctgcggc gctggatctg
    10861 gcttacgtcg ctgcgggtcg tgttgacggt ttctttgaaa tcggtctgcg tccgtgggat
    10921 ttcgcggcag gcgagctgct ggttcgtgaa gcgggcggca tcgtcagcga cttcaccggt
    10981 ggtcataact atatgctgac cggtaacatc gttgctggta acccgcgtgt tgtgaaagcc
    11041 atactggcga acatgcgtga cgagttaagc gacgctctga agcattaatg actcaggcgg
    11101 gtgatatcac tcacccgccc tcgcctttca ggcgctattc cgaaatactt cctcaccgct
    11161 ttactttctt tcgtcactct cccaccatct ttccccgatt aatggatgat aaagaagtcg
    11221 caaggaaagg ttatggcact gccagtgaac aaacgcgttc ccaaaattct gtttattctc
    11281 tttgttgttg ccttctgcgt ttatttagtg ccgcgcgttg ccatcaactt cttctattat
    11341 cctgacgata aaatttacgg tcccgacccc tggtcggcgg aatccgtcga atttacggct
    11401 aaggacggta ctcgtctgca aggctggttt attccttctt cgacgggccc tgctgacaac
    11461 gccatcgcaa ccatcattca tgctcacggc aatgccggaa atatgtccgc ccactggccg
    11521 ctggtcagtt ggttacctga gcgtaatttc aacgttttta tgtttgatta tcgcgggttt
    11581 ggtaaatcaa aaggcactcc gtcccaggcc ggattgctgg acgatacgca aagcgccatc
    11641 aatgtggtgc gccatcgcag tgatgtaaac ccacaacagc tggtgctgtt cgggcagagt
    11701 attggcgggg cgaatattct tgctgttatt ggtcagggtg atcgtgaagg catacgtgcg
    11761 gtgatcctcg actccacatt tgcctcttat gcaaccatcg ccaaccagat gatccccggc
    11821 agtggctact tacttgatga gagttacagc ggcgaaaatt acatcgccag cgtcagcccg
    11881 atcccgcttt tactcattca cggtaaagcc gatcacgtta tcccatggca gcacagcgaa
    11941 aagttgtata gcctggcaaa agagccaaaa cggttgatcc taatcccgga tggcgaacac
    12001 attgatgctt tttccgatcg tcacggcaat gtttatcgcg aacagatggt ggactttatc
    12061 cttagcgcgt tgaatccgca gaactaaccc atgatcgcta gcacgataat cattcacaaa
    12121 accaccttaa gacatgctaa tccactggtc agaacagttt aaaatgagaa aaattctgtg
    12181 acgcttgcca acatttctga tgattagcat tcccttcgcc atttccctga gcaaacttta
    12241 gctattctta tcaattatgc ttatgggaga tctacagatg atgcctacgc ttgctccacc
    12301 atctgtcctt tcggctcccc agcgccgctg ccagatcttg ctgacgctct ttcagccggg
    12361 gttgaccgcc accatggcaa ccttcagcga gcttaatggt gtggatgatg atattgccag
    12421 tcttgatatc agcgaaacag gacgggagat cctgcgctat catcaactca cactgacgac
    12481 tggttatgac ggtagctacc gggttgaagg tacagtgctt aaccaacgtt tgtgtttatt
    12541 tcactggcta cgacgtggtt tccgtctgtg tccgtcattt attaccagcc aattcacccc
    12601 cgccctgaag agtgaactga agcggcgcgg aattgcgcgt aacttttacg acgataccaa
    12661 tctacaagcg ttagtgaatc tctgctcccg acggctgcaa aaacgctttg aatcgcgcga
    12721 tattcatttc ctgtgtctgt atctgcaata ttgtttgctg cagcaccacg ctggaattac
    12781 gcctcagttt aatccgctcc aacgtcgctg ggcggaatcc tgccttgaat ttcaggtagc
    12841 gcaggaaatt ggacgccact ggcagcgtcg ggcgctccag catgtaccgc ctgatgagcc
    12901 actgtttatg gcattacttt tttccatgtt gcgggttccc gatccactgc gggatgcgca
    12961 tcagcgggac agacaattgc gtcagtctat caaacgtctg gtaaaccatt ttcgtgagct
    13021 gggaaatgtt cgtttttatg atgaacaggg gttatgtgat cagctttata tccacctcgc
    13081 ccaggcgtta aatcgcagtt tgtttgccat cggtattgat aataccctgc cggaagagtt
    13141 cgccagactg tatccacgcc tggtacgcac cacccgcgcg gcgctggccg gatttgaaag
    13201 tgaatacggc gtccatcttt ctgatgagga aagtggtctg gtcgcggtga ttttcggtgc
    13261 ctggctaatg caggaaaacg atctgcatga aaaacagatt attctactga ccgggaatga
    13321 tagcgagcga gaagcgcaga ttgagcagca gttacgcgaa ctaacgttac tgccgctcaa
    13381 cattaagcat atgtcggtaa aggcattttt gcagacaggc gctccgcgtg gcgcggcact
    13441 gattattgcg ccttatacca tgccgttacc gctcttttca ccaccgctga tctatacgga
    13501 cctgacgctg acaacacatc aacaggagca gatccgcaaa atgctcgaat cagcatgagg
    13561 gaacaacttt cgggcgcaaa aacattgccg gaagcgccac cagcgccatt acccagaaca
    13621 cgccgtggcc cagatattga tacaggaaac cggcgaaaac ggtcatgata gcgatactgc
    13681 cgcccatcgc gacggcagag taaaccgcct gtaaacggat gacttcgcta acctggcgag
    13741 cagcaatata acgcatggcg gccaggtggc agaccgtgaa ggtgccgcaa tgcagaattt
    13801 gcaccactat caaccacggc aacgccgtag ttgctcccat aatgccccag cgcactacgc
    13861 cgcaaatcgc cgagatcaac agcatatcgc gagcactaca acggcggaaa agtttattac
    13921 tcagcgcaaa gataatgact tccgccacca cgcccagcga ccacaaatac cccaccgccg
    13981 aggccgagta gccagctgcc tgccagtaaa tggcgctaaa accgtaatag gccgcatgtg
    14041 ccccctgcaa taaacaaacg caggccagaa agcgccagtt ctggcgaacc agcgccaacc
    14101 acgcagacca accggtgctc tcctgctggc ggcttgcccc ttgtggctga atcgtcggac
    14161 ggatgagaaa gccgagcagc atggatgcca cgcccaacgt caacagcacg agaatcaccc
    14221 gataatcaaa catagtgacc agtttgcccg tcagcgccga gccaatgaca aacgccaccg
    14281 agccccacag tcgcactttg ccgtaatcaa gcgggaactg cttttgccac gtattcgcca
    14341 gtgcatcggt caacggtacc agcggtgaga aaaagaggtt aaagccaatc atcaccagca
    14401 tcagccacgc tacgtgcgcc cccgcccaga aggcgacagc aaagagaagt ggcagcagtg
    14461 ccagcacgcg caaggcggaa atcaggcggg aaggatcgct gacgcggggc gcgatgagca
    14521 aactcccgag aaaacgggca accagacctg cccccaataa caggccgatg gtttctggcg
    14581 ttaaaccaat ccctttaagc cagacgctcc agaaaggtaa aaaaatgccg taactaaaaa
    14641 agtatgtgaa atagccgagc gccaaccagc gcgtggattg caaaaccatg agtccctccc
    14701 gtcaggaggc gttagtctgg ggttaatcga atgttggtgc aagttgaaaa ataagaatat
    14761 tcgagcctgc ttcccgatgt ctgtaacgcc agttcacaag cactaaaaaa ctgcgatccg
    14821 cgacgcatgt tgcaaaaata atgcgtaatt atgccgttac gcttgccaaa cgttcctgca
    14881 caatggcgat gaaatcgcgt aatgccagct tcatctcccc tttcttccac gccatcagta
    14941 aagcaatgga aggcacattt ccggcaatgg gacgaaaaac aacctgcccg gtattaaaat
    15001 tattcatata accgggtatc aaagtgacgc ccagccccat gcccaccaga ttcatggtca
    15061 ccagaatatt cgttgccacc tggacgatat ttggctggct gttttcttgc gcaaaccacg
    15121 ctttaacgat cggcgcaagc gaaccggaat acgccggatc ggtactgacg aaattcacgc
    15181 catccagttg cgcggcggtg atctcttttt catgtgctaa agggtgatca accggtaaca
    15241 ccaccactaa tggttcgtca aaaagctcca gataatcaat ctccgggcta taaacaggat
    15301 ggcgcattaa gccgacgtcg agttcaccac ggcgaatttt ttcctcctgt tgcgtggtga
    15361 ttaaactcac cagctcaatc aaggtgtctg gctgtctgag acgaaacatc ggtaatactt
    15421 ttggcaataa attcacttcc gccgatggca caaagccaat ggttaattgt ctgtcttcct
    15481 gaacaatttt ccgcgcccgt aatttggcat tttccgcttg ttcgaggatt gccagcgcgt
    15541 cctggagaaa acattctccc gccgccgtca acgcgacttt gcgcttatcc ctcaccagta
    15601 acggaacacc gacacagttt tcaagatcgc ggatctggct gcttaacgaa ggctgtgagg
    15661 tatgcagttt ttccgccgca cgggtaaagt tcagtgcctg cgccactgcg acgaaatagc
    15721 gtaaatgccg tagttccatc accttcccct tgttatcgaa aaaacgtctc agctggtaga
    15781 aattaactat ttcacatatt agcaaccaac cagcaacatc cttatggcac aaaaatagaa
    15841 ggtcaataca tcttatcttt caggattaaa aaatatgacc acaccctcag atttgaacat
    15901 ttaccaactg attgacaccc aaaacggtcg ggtcactccg cgtatttata ccgacccgga
    15961 catttaccaa ctggagcttg agcgtatttt cggtcgttgc tggctatttc tcgcccacga
    16021 aagccagatc ccaaaacccg gtgatttctt taacacctac atgggagaag atgcagttgt
    16081 cgtggtgcgc cagaaagacg gcagcatcaa ggcgtttctc aaccaatgcc gccaccgggc
    16141 catgcgtgtg agttatgcag attgtggcaa cactcgcgcc tttacctgcc cgtatcacgg
    16201 ctggtcttat ggcattaacg gcgagttgat cgatgtaccg ctggaacctc gcgcctaccc
    16261 acaagggttg tgtaaatccc actgggggct aaacgaagtt ccttgtgtgg agagttataa
    16321 aggactgatt tttggcaact gggataccag cgcaccgggc ctgcatgatt acctgggtga
    16381 cattgcctgg tatctggatg gcatgctgga tcgtcgcgaa ggcggcaccg aaattgtcgg
    16441 cggcgtacaa aagtgggtga tcaactgtaa ctggaaattc ccggcagagc agttcgccag
    16501 tgaccagtat catgctctgt tcagccatgc ttctgccgtt caggtattag gggcgaaaga
    16561 tgatggcagc gataagcgcc tcggtgatgg acaaaccgcc cgcccggtgt gggaaaccgc
    16621 caaagatgcg ctgcaatttg gtcaggacgg tcacggtagc ggtttcttct ttactgaaaa
    16681 accggatgct aatgtctggg tcgatggcgc agtttcaagc tattaccgcg aaacctatgc
    16741 cgaagcagaa caacgtttag gtgaagttcg cgccctgcgc ctggcgggtc ataacaatat
    16801 attccccacg ctttcatggc taaacggcac tgccacactc cgcgtctggc atccgcgcgg
    16861 ccctgatcaa gttgaagtgt gggcgttctg tattactgac aaagccgcct ccgatgaagt
    16921 taaagccgct tttgaaaaca gcgccactcg tgcttttggt cctgctggtt ttctcgagca
    16981 ggatgactcg gagaactggt gtgaaatcca gaaattgctt aaaggccacc gcgcccgcaa
    17041 cagcaaactg tgtctggaaa tggggcttgg tcaggaaaag cgccgcgacg acggcattcc
    17101 tggcattact aactatatct tttcagaaac tgccgctcgt ggaatgtacc aacgctgggc
    17161 cgatcttctg agtagcgaaa gctggcagga agtgctcgat aaaaccgccg cttaccagca
    17221 ggaggtgatg aaatgagtgc gcaagtttca ctggagttac atcaccgcat tagccagttt
    17281 ctctttcacg aagccagtct gctggatgac tggaaatttc gtgactggtt ggcacagctc
    17341 gacgaagaga ttcgttacac catgcgcacc acggttaacg cgcaaacacg cgaccgtcgc
    17401 aaaggcgtcc agccgccgac gacctggatt ttcaatgaca ccaaagacca gctggagcgg
    17461 cgaatcgccc gtctggaaac gggcattgcc tgggcagaag agccgccgtc acgcactcgt
    17521 cacttaatca gcaactgcca ggtaagcgaa accgacatcc caaacgtatt tgccgtgagg
    17581 gtgaattatc tactttatcg ggcacaaaaa gagcgcgatg aaacattcta tgttggaacg
    17641 cgtttcgaca aagttcgccg tctggaagat gacaactggc gcttgctgga acgggatatc
    17701 gtcctggatc aagcggtgat cacttcccat aacctgagtg tactgttctg atgaatcgaa
    17761 tttatgcgtg tcccgttgcg gatgtgccgg agggtgaggc tctccggata gatacctcgc
    17821 ccgtcatcgc cttgttcaac gtgggcggcg agttttatgc cattaacgat cgttgcagcc
    17881 atggtaatgc gtcgatgtca gaagggtatc tggaagatga cgccacggtg gagtgcccgc
    17941 tacacgccgc cagtttttgc ctgaaaacgg ggaaagcgtt atgcctgccc gccaccgatc
    18001 cgctcaccac ttatccagta cacgttgaag gtggtgacat tttcatcgac ttaccggagg
    18061 cgcagccatg agcgatctgc ataacgagtc catttttatt accggcggcg gatcgggatt
    18121 agggctggcg ctggtcgagc gatttatcga agaaggcgcg caagttgcca cgctggaact
    18181 gtcggcggca aaagtcgcca gtctgcgtca gcgatttggc gaacatattc tggcggtgga
    18241 aggtaacgtg acctgttatg ccgattatca acgcgcggtc gatcagatcc tgactcgttc
    18301 cggcaagctg gattgtttta tcggcaatgc aggcatctgg gatcacaatg cctcactggt
    18361 taatactccc gcagagacgc tcgaaaccgg cttccacgag ctgtttaacg tcaacgtact
    18421 cggttacctg ctgggcgcaa aagcctgcgc tccggcgtta atcgccagtg aaggcagcat
    18481 gattttcaca ctgtcaaatg ccgcctggta tcctggcggc ggtggcccgc tgtacaccac
    18541 cagtaaacat gccgcaaccg gacttattcg ccaactggct tatgaactgg caccgaaagt
    18601 gcgggtgaat ggcgtcggcc cgtgtggtat ggccagcgac ctgcgcggcc cacaggcgct
    18661 cgggcaaagt gaaacctcga taatgcagtc tctgacgccg gagaaaattg ccgccatttt
    18721 accgctgcaa tttttcccgc aaccggcgga ttttacgggg ccgtatgtga tgttggcatc
    18781 gcggcgcaat aatcgcgcat taagcggtgt gatgatcaac gctgatgcgg gtttagcgat
    18841 tcgcggcatt cgccacgtag cggctgggct ggatctttaa ggaagcacga tgaaagaaaa
    18901 aacgatcatt attgtcggtg gcgggcaagc ggcggcaatg gctgcggcct cgctacgcca
    18961 gcaagggttc accggtgagc tgcatctgtt ttccgatgag cgacatcttc cttatgaacg
    19021 acctccactc tcgaaatcca tgttgctgga agattccccg cagttacagc aggtgttacc
    19081 cgctaactgg tggcaggaaa acaatgttca tctgcattcc ggtgtaacca tcaaaacgct
    19141 gggtcgcgac acacgagagt tagtgttaac caacggcgaa agctggcact gggatcagct
    19201 ttttatagca accggcgcgg cagctcgacc gctgccgttg cttgatgcac tgggagaacg
    19261 ctgctttacc ctacaccatg ccggtgatgc cgccagactg cgagaagttc tgcagcccga
    19321 acggtcagtc gtgattgtcg gtgccggaac tattggtctg gaactggctg ccagcgccac
    19381 gcagcgcaga tgtaaggtga cagtgattga actggcggca accgtcatgg gccgtaatgc
    19441 accaccgccc gtgcaacgct atcttttaca gcgccatcag caggctggtg tgcgcattct
    19501 gctcaataat gccattgaac atgtggtcga tggtgaaaaa gtagaactga cgctgcaaag
    19561 tggggaaacg cttcaggctg atgtggtgat ttacggtatt ggtatcagcg ccaacgagca
    19621 actggctcgc gaggccaacc ttgatactgc caatggcatt gtcattgatg aggcttgccg
    19681 cacctgcgat cccgcgatct ttgccggtgg cgatgtggca atcactcgtc ttgataatgg
    19741 tgcactacac cgctgcgaaa gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc
    19801 cgcaatgtta gggctaccgc taccgctact gccgccgccg tggttctgga gcgatcagta
    19861 cagtgataac ttacagttta ttggcgatat gcgtggcgat gactggcttt gtcgtggcaa
    19921 cccggaaact cagaaggcga tttggtttaa tctgcaaaac ggcgtgctta tcggtgcggt
    19981 cacgctgaat cagggccgtg agattcgccc aattcgcaaa tggatccaga gcggcaaaac
    20041 gtttgatgcg aaactgctga tagatgagaa catcgcgctt aaatcactgt aatcaggata
    20101 attagcgaat atctcaatgc ctggggcgtg gcgaggtgca agagtgtgta ttacgtttaa
    20161 atcacattat cttgcaaagg gattggttat gaacacacta cgttattttg attttggagc
    20221 tgcccgcccc gttttgttat taattgcccg tatcgccgtg gtcttaattt tcattatttt
    20281 tggttttccc aaaatgatgg gctttgacgg tacggtccaa tatatggcct cgttgggcgc
    20341 gccaatgccg atgctggcag cgattattgc ggtagttatg gaagtgcccg ccgcgatatt
    20401 aatcgtgctt ggctttttca cccgtccgct ggcggtgctg tttattttct acacgctggg
    20461 tacggcggtg attggtcacc attactggga tatgaccggc gatgcggttg ggccaaatat
    20521 gattaatttc tggaagaatg tcagtatcgc tggcgcgttc ttgctattgg caattaccgg
    20581 accgggggca atttctctcg atcggcgtta ggaaagatgc cggatgcggc gtgaacgcct
    20641 tatccggcat tttataaatt acagccactc tacccgcaac gacatctctg aggttgttga
    20701 ttcccctggt gctagcgcaa tgagatcacc cccttccggg cgatgatgat catccggcgc
    20761 atggctcatc ggttccagac agaagaaatc aaacgcatat cctttatcaa acgcagggtc
    20821 ggaaacaaag atgaaataac acggtgcagg tggcgtcgtt tccatgatga tggcataacc
    20881 ttcctgcggc tgctcgatgc gggcctgacc attccatccg gcaaaaccat tgttcaccca
    20941 ctgacgcggc aacggcgcga gctggttaaa atccagttcc tgcggtagct gctcgcaaaa
    21001 ctcacccgcc agccactgct cccgttccag ccagtaaccg ctcgcctgcg cctgaatccg
    21061 cgtttgtggt gacaacggaa aataaggatg ccagccggta ccaaatggca gcgtctctgc
    21121 cccttgattg gtgacagaga gcgtcaccgt cagcgtatcc gccgttaaat gaaacgcctg
    21181 acttacccga tagtgataga caccgctgcg atgttcatac accagacaca aactatcatc
    21241 gctgtgcgag acacattgcc actcgcccag ccagccatcg ccgtgtagat agtgcgcatc
    21301 ccactcaaca ttcggttgca gttgatactc acgcccctgc cagacaaaac ggttgccgct
    21361 cacccggttt gcaaatggca ccagcggaaa acatgaggca tcggttgcca caccgctttt
    21421 tttaccagga cgtaagagcg gcgtcgtatc gcgccagaag ccttcgatta cgccgcccta
    21481 gtcagaaacg tccagcttta gcgacccgtg ggataaggta tagatggtca tccgctaact
    21541 ccttaatccg ggaagttaat cacaactttc ccgcatttgc cgctcgccat cagcacatag
    21601 gcatcacctg cctgttctag cgagaagcga tgggtaatgg cgttacgcgg ccacagcttc
    21661 cagtcagtca aatcgtgggc gcatttttcc atatggaaca gactggtcac ccaggagccg
    21721 ataatccgcc gttgatggtg catcagatca gcgctgacct cgaattccac ttttccggtt
    21781 tcaccaatgt aaaccacccg cccccagtca gcagtggatt gcagtgccag caagcgacct
    21841 gcggcattac cggaacaatc gagcgcaaca tccgcgccac cgtgggtaag ttcagcaatt
    21901 atctgcggca gaccttcggt ggttgctaaa tagccgtgat ccatcccccc taactgtttt
    21961 gccatcgcca gacgttccgg cagcatatca acgccgatga tccgttttgc accgcgacct
    22021 ttcgccagca tcatcgccat catgccgacc ggccccagac cgaccaccag cacgttatcg
    22081 ctgccggaaa cttcgccgcg caaaatccct tcataggccg tgccaacgcc gcaactgata
    22141 aacgcaccat cttcgtagct cagcgcatcc ggcaggagga tcagatcttt ttcttccgcc
    22201 agcagatatt cggcatgacc gccgtcacgc tgccagccgt aagccgcttt tcctttgcca
    22261 gtacaagaga taggaaagcc gcggcggcag ttcgggcaaa aaccacagcc agaaatgtga
    22321 tacaccagca cgcggtcgcc ctctttaaaa tggcggcagc cttgccccat cgccacaatc
    22381 tgcccgcacg gttcatgacc gttgataaat ccctggtata acggtttatc gggtgccgcc
    22441 gccgtggcac ggtgttgatg atagatatag tggacatcgc ttccgcaaat cccggaggat
    22501 ttcattttga tcagtacctg gttaatcccc ggcgtcggca ccgcaacttc ccgcagatcg
    22561 acggttgaat ttcctagtaa ataagcagcc agcatcgttt tcataaatcc ctcattaaca
    22621 atacgattaa ttttcatccc tgccgcaccc gcgccagggg cgttaaggtt tagcgtttcg
    22681 ctttactgct tcgctgggtc agcaagatat tcgccagcac cgccaccacg atgatgacgc
    22741 cgcgtaccac ctgctggaaa aaggagttaa taccgagcag caccagaccg ttaccgatta
    22801 gcgtaatcac cagcacacca agcaatgtac cgaacaggga accgcgaccg ccggaaagtg
    22861 ccgtaccgcc gaccacgacc gcggcgatga cgtcaaactc cagaccgttt gcggcacctg
    22921 cgttaccgga accgaggcgt gccgccaaca aaatgccggt caccgccgct aataatcccg
    22981 aaagggtaaa gataagaatg cgcactcgac gaacgttaat accgcacaac tgcgccgccg
    23041 tggcattacc gccaacggca aaaaccgagc gcccgaaggc ggtttttcgg ctaatgaaca
    23101 caaacagcgc aaacaacacc atcatgatca gcgcggatac tggcacaccg agaaattgtc
    23161 cgcccagcca gtccagcacc tcgttttcgt caatcggcac tggcagtgcg ttggtcataa
    23221 acagccccat tccgcgcaga gcgctccaca gccccagtgt ggcaacaaaa cttggtacgt
    23281 taaacacgcc gcgcagcacc ccggcgagcg ttcccatcag cgcgcctaac agcaacacca
    23341 gcagacaagc aatcgccaac ggaacttcaa attgcagcaa aaatgccagg cacaccgaga
    23401 caaaagccac catcggccca acgctgacat caatttcacc ggagataata atcagcgtca
    23461 tcgcccaggc ggcaatccca atggtggcgg catcgcgcag cacgttcatc tggttattca
    23521 atgagataaa gccaggcgcg ttcagggaga agaccagata aagaatggca atcaccacca
    23581 gcaaaccgat ctcattaata tggcgactga caaattgttt gagcgagacg ctcttgccct
    23641 gcggcaatgg taatgacgaa gcatacatct tcaggttcct cggtaaaatt catcagtgta
    23701 cagacagaat ggcggacatc agctcatcca cattgaccgg cgagtgaaac tcctgcgaga
    23761 acgtgccgtg ctgtaataac aggatgcggt cacacaccag cggcaattcc tccacttcac
    23821 tggagataaa caccacgctt tttccttcgg cagccagctc acggacaata cggtaaatct
    23881 gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc gagcaacaaa atctggctgg
    23941 cggcatagac ccaacgaccg atcaccactt tttgctgatt gccaccagaa agcgtgccga
    24001 tgggtgtttc gctactggca gccttgaccg tcatccgctg catcacctct tcggtcaggc
    24061 ggcggatggt ggaccattgc agcacaccgt tggtgctgat tttttgccga ttggtcagca
    24121 ctgtattttc gtcaacgcct agccagggaa tgatccccgc ttctttgcgg ttttctggcg
    24181 tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg gcgcatgatt ttctcgccgt
    24241 tgataacaat ttcgccctgt tcatacgtct ccagcccaac aatcgccttc agcaattcac
    24301 tgcgccctgc ccccagcagg ccagcaatgc caagcacttc gccacgacgt agcgtaaagc
    24361 tgatatcctc cagcttgggc ttatggcgta acgcacggac ttccagtacg gcctgatcca
    24421 caatttcctg aggggcaacc ggcgcaatat caacgtgatc acgccctagc attagcgaca
    24481 caatatgatg cgtggaggtg ttttcgagca tcacatcgcc cgccacctga ccatcgcgca
    24541 taacggtggc ataggaggca atgcggcgga tttcttccat ccggtggctg acataaatca
    24601 ccgccacgcc cagtgccgac atctttttca ccgcactgat caccagttca acttctgcac
    24661 tggcaagcga gctggtgggt tcatcaagaa tgaccacgcg cggctcgccc ttcatcaccc
    24721 gtgcaatttc caccagttgc ttttgcgccg ggcttagcgt tgaaacaagt tgttcaggac
    24781 taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc ctgcgccatt tgcaggtaat
    24841 caatcatgcc gttgcggcga ggccattgac caaggcagag gttttctgcc accgtcagcc
    24901 cttccaccag acttaattcc tgataaaccg cacgaacccc cagttcagcg gcacggcgag
    24961 tcaacgtagc ttcgtcacct tccagtcgcg tctcgccaat ccagatatca ccgctatccg
    25021 ggcgttcgct gccggtaagc attcgaatga gagtcgattt gcccgcgccg tttttaccta
    25081 acagcgcacg aacttcgcct ttattgagcg tgaagttaac gttatccagc gcaacgacgc
    25141 cggggtaacg cttatttcct gccaccactt ttgctaccgg gactgcctct gttgccgtga
    25201 acatagggac ctctgcgaat cagcgattag ggcagaccat caacgtgcgt tgccagccac
    25261 tgtttaccat cttccgtttt ggtatagaga tcgattggca cctgaatcac tttttcaccg
    25321 tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa cagcattgcc cattttctta
    25381 ccggaaatat ccactaccgc tttcagcacc tgattgtttt ccagctcctg agcaatttcg
    25441 gttgtcatat ccgaaccgaa aacagcaatt tttccggcct gattttgatt acgtaccgct
    25501 tttaccgcgc cgagtgtcgc accgcccgac tcccccataa tggcgttgag atccggcgtg
    25561 gagataatca gtttttcacc aacggaaatc gctttatcta aaacagtccc ttcctggtta
    25621 gcgacaattt gcgcgccggg aacgcgggat tttaatactt cttcaaatcc tttacgtcgc
    25681 tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa ttttcggctg gtcaatttta
    25741 ttggcaataa aataatcggc ggcagcgtta cccagttttt taccaaattc cagtggatcg
    25801 ccgaccagat acgccgagac atatttatcg acaccctttt gattaataca ggtgttgtag
    25861 caaatcaccg gaatacccgc ttcactggcg cgacgaacgg tacggctact gccattttca
    25921 gacactgccg ataaaataat ggcatcgaca ttacgcgcca cgagggtatc aacaaaggta
    25981 ctttctttcg aaatatcccc ctgggcgtta gtttcaatta actgtacctg tactgaagaa
    26041 tcttttgccg catcctgaac gccctgacgc actccagcgt aatatccctg ggtatcaagg
    26101 tatattgcac caatggtcat ttctttatcc gcagccctgg caaatagtgc gctacctaat
    26161 agcgtagcca ttaataatag attacgggtt gttctcattt ttttaggcat agtgcctcct
    26221 gtagggtttt tattaacaac ggcttattct aattattttg tgatgagcgg cagcgcgtgc
    26281 cgctcaaata ttacagttaa tagggtttag tgaacgtagt taaaaataaa aggcatcaca
    26341 gtggtgaata atgccgcttt cggccaggcc ggattaatcg ccgtcaattc agcgcgagca
    26401 gactcatatt gcgcaacctc cccgagtccc gcggacgcca gcatcgccac catcaggcat
    26461 ttttctttat gctgctgttg taaatcgcca tacagcgata acaggtcagg ttgtgaaaca
    26521 gcaaagaaat ccgcctcaat actggttttc gccatctctt gcgcccactg tttcatttca
    26581 ctaaacagtt gctgtgcggt ttgctgttca cccagcagtc gcagcgccat tccttgccag
    26641 aagagataat caaccggctg atcgttgtaa taactgtgga tgttaatggt gcgatcgccg
    26701 gtcgccgcca gacgtaaaca acgcatcgct tcagtttcat cgccctgcgc gttggcgcat
    26761 atcgcctgcc agaaccagat gtcgttatca gtttgccccg gtaaacggcc ttcgcttaaa
    26821 ttctccggat aatacagcgc ggcatgaagc agttcgctgg cctgctgcgg ctctctggca
    26881 tcaagatgct gccaggcgcg taataactgg ttgaggataa actgactggt gaccttccct
    26941 tccccgcctt cccacgggta gaatttacgc gtggcgagaa tgtccgccgc tttgtctgcc
    27001 tgacccgtga gatgccacaa attgagcagt tctgcggtca tgtcgtcgcg tttcagcgca
    27061 atttccagat tattttccag acgcgccagt cgtttctccg gtgtggctcc acttaactta
    27121 tccagcaaat cccgttcgaa aagcagacgt gcatcctgcg gcgcaagctg ataagcatta
    27181 tcaagataac gcgcggccag ctcataatcg tgtttcttat tccacgcatg gatcgctaac
    27241 ccgcgccagc cgtcggcaaa ctccggcgac atctctacgc aacgttgcca taaggcaatg
    27301 gctttgttgt agctacgttt gttgtagtag aagcaggcca gtaaatggcg agcaaaccag
    27361 cactcttcaa tactctccag cgccgccact tcttccagcg tattcgggaa acggacaaac
    27421 tgcgggaaga catcaatggc ttttgcgacc agttcgccac gttcggcttt cggcagcaag
    27481 ctggcttgca ggtaaagcgg cagcgtgcgc tggcagtcca gtgcgttcag catctctgcc
    27541 gccagggtgg gcattcccca gttaatcagt tgcccggcgg tcatcagagc gttaacgtcg
    27601 cgtccctgac acagaccgcg ccactgcgcg agggctgatt cgctacgacc atcgaaccag
    27661 ttcagccacc acagagtggc gttcagcgga taatcacgca gcagtttctc gcgctgcaaa
    27721 cgcgcgttgt cctgacgacc acttaacacc agcagcagat tatgcaagca aagcacttcc
    27781 tgattggttg ggcaggcacg aagactttgt tggcaaaaat ccagacccgc gtcgaagtta
    27841 tcattacgcg ccgccagtcg tgccagacca taatagccgc ctgctttact attgccgctc
    27901 cagaccgcac gccagaaatc ctcttcggct tgttgatatt gtccctgacg ttcgtaagca
    27961 ctggcgcgaa tcaaacttgc ttgtccacac tgcgggtttt tgttcagcgc atgtgcgcgt
    28021 ttcagcgcct gactggcata cgccaccgct tgcgggaaat ctgcgcggtt atattccagc
    28081 atcgccagcg ccaggttaca gcgataatcc agcgggtcca gcgccacgcc gcgcaggtag
    28141 taatcgaacg gtgaacggct ggcgtgatga tattgctcca gatgctgacc gataaaccag
    28201 gcttcatctg tactggtaat gtcttgtgct gccagtggcg ctttggcgac gtccggcaac
    28261 ggcaactcct gcggctgatg ttcctgataa ctcagtacaa tattgccgtc ggcatcggag
    28321 agttcaatgg tcagtctgtc aggattgata ccgtgcaaca cgccctggat ggcggtcgct
    28381 ggtgtcaggg ccacggcatc atcgagcaac gcgttgcatt tgccgatttc gcggatcgcc
    28441 agacggtatc cgttcaacgg agagatggca tacagccccc actcaatccc ccgctcacta
    28501 cgctggagtt ttatcaccgc atcgcgggag gcattttgca ccatgcccag agaatgataa
    28561 ggcaggaaat actgctcgaa acgcttctct tcgtaagcat caagccaggt aaaatcaggc
    28621 tggttatcgg caaaaatacc ggtcatcagt tcgatatacg ggccgttatt gtcggtcaga
    28681 ctcttatccc acgcctggcc aaattcactg tgtccccaac tccactgttt tttacctggc
    28741 gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt catcgtgaca ccacgcgccg
    28801 acaaaatcgt actgcgattt ttcggccata tatgaggttg gaacaggcac atttttatag
    28861 cgagaaatgt ccactccggc ggagtagtcc actttgtagt aagtgccggt ggcgatgggg
    28921 aaagcggaga cggcccgttt gccgtgatca aacaccgcag tcacatccgg tgggaaaacg
    28981 ctctgatgcc cttcaccccc tttcactgcc gggttggccc accacaagaa atgacgcggc
    29041 gtggcgttgc cgttatagac gcggctggcg atttccagtg ccgcccggtc agggcgcagg
    29101 gtgaaacctg tcatcacctg taaaccatgc atcggctccg tttcgccgac ccacaccgtc
    29161 tgtgcaccgt cttcatgggc ttcgagggtg aaatcaacgg gcataaaggt ggtcgggcga
    29221 tggtgttgcg gccagttaaa ctcaatcccg ccggagatcc acggtcccag catccccacc
    29281 agcgcaggtt taatgacttc attgtgatag acaaaatcgc gctgtttcac tttatcccat
    29341 gcgcgatgca cccgaccgcc cagttccggc aggatcatca ctttgatgta gtcgttttcc
    29401 agccacaccg cctgctagga tttcagggtt ttctgctcgc tcagcgtatc ggtcacgccg
    29461 tagggataaa ccgcgccgga cgatccctga taaacgcgat tttccaggaa catgggatgt
    29521 atatcctgcg gcccggtttc ataggtcggg atctcaacgc gctcttgcca cacttttact
    29581 ggagtcatgg tgccctcaat attaacaaga catactgaat taaaagattt gtggcagtgt
    29641 attgaacaat ctggcaatgt tttcgcggaa taatcacgca attaactaaa caaggtttag
    29701 tgaagatgag agcctgcatt aacaatcaac agattctcca ccataacaaa tgcgtgattc
    29761 tggaactgct gtaccggcaa aagcgcgcca ataaatcaac gctggcccgg ctggcgcaaa
    29821 tttcgattcc ggcagtcagt aatattttgc aggaactgga aagcgaaaaa cgggtggtga
    29881 atattgacga tgaaagccag acgcgcgggc atagtagcgg tacatggctg attgcgccgg
    29941 aaggtgactg gacgctgtgc ctgaacgtga cgcccaccag tattgagtgt caggttgcta
    30001 atgcttgttt aagtccgaaa ggcgaatttg agtatttaca gattgatgta ccgacgccgc
    30061 aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca ccgtaaattg tggccggacc
    30121 gcaccatcaa tctggcgctg gcaatccacg ggcaggttga tccggtgacc ggcgtatcgc
    30181 aaaccatgcc gcaagcgccg tggacaacgc cagttgaggt gaagtatctg ctggaagaga
    30241 agctgggcat tcgggtgctg gtcgataatg actgcgtgat gctggcgctg gcggagaaat
    30301 ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa cgttgattac ggcattggct
    30361 cgtcgttcgt gattaacgag caaatttatc gcggcagttt gtatggtagc ggacagattg
    30421 gtcacaccat cgttaatccg gatggcgtcg tctgcgactg tggacgttac ggcagcctgg
    30481 aaaccgtcgc ctcgttaagt gcattaaaaa aacaggcgcg ggtatggcta aaatcacaac
    30541 cggttaatac tcagcttgag cctgaaaaac tgacaaccgc gcagttaatc gctgcctggc
    30601 agagtggaga accgtggatc accagctggg ttgatcgctc tgccaatgcc attggtttga
    30661 gtctgtataa cttcctcaac atcctcaata ttaatcagat ttggttgtac ggtcgcagtt
    30721 gtgcctttgg tgagaactgg cttaatacta ttattcgcca gacaggattt aacccgttcg
    30781 accgcgacga aggaccgagc gtgaaagcga cgcaaattgg cttcgggcaa ttaagccgcg
    30841 cacaacaggt gctgggaatt ggctatttgt atgttgaggc gcagttacga cagatttgat
    30901 ggcgcgataa cgtagaaagg cttcccgaag gaagccttga tgatcataaa cgaaaaattg
    30961 cctgatgcgc tacgcttatc aggcctacac ggagattgca atatattgaa tttgcaagat
    31021 tttgtaggcc tgagaaggtg ttcatgccgc atccaacatg aacaacgagc acattgtcag
    31081 caaatcaccg tttcgcttat gcgtaaaccg ggtaacgtgc gcagatgtcg agaactttac
    31141 ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc cagcacgtca cacatccagc
    31201 cagccagttc tttcgcttcg gcttctttaa agccgcgacg ggtaatcgcc ggagtaccta
    31261 cacgaatacc ggaggtcaca aacgggctct tcggatcgtt cggtacgctg tttttgttga
    31321 cggtgatgtt agcacggccc agagcggcgt ctgcttcttt accggtcagg tttttatcaa
    31381 ccagatcaac caggaacagg tggttatcag tgccgccgga aaccacttta tagccgcgct
    31441 cgaggaacac ttctaccatc gctttagcgt ttttcgcgac ctgctgctgg taagttttga
    31501 actcaggctc catcgcttct ttcagagcaa ccgctttacc ggcgattacg tgcatcaacg
    31561 gaccgccctg accaccaggg aaaacggcag agttcagttt tttgtacagc tcttcgctac
    31621 cacctttcgc caagatcagg ccgccgcgcg gacccgccag ggttttgtga gtggtggtag
    31681 taacaacgtg agcatgagga accgggttcg ggtagacgcc agcagcaacc aggcccgcaa
    31741 cgtgcgccat atcaacgaac agataagcac cgatgctgtc agcgatttca cgcattttcg
    31801 cccagtcaac cacgccggaa tatgcagaga aaccaccgat aatcattttc ggcttgtgtt
    31861 ctttggcttg tttttccaga tcggcgtaat cgatatgacc ggtagcatcg ataccgtaag
    31921 gaacgatgtt gtacagttta ccggagaagt taaccggaga accgtgagtc aggtgaccgc
    31981 catgcgccag gttcataccc agaacggtat cacctggttc cagcagcgcg gtgtagaccg
    32041 caaagttagc ctgggagccg gagtgcggct ggacgttagc gtagtcagcg ccgaacagtt
    32101 ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac atactcgcaa ccgccgtagt
    32161 agcgtttgcc cggataacct tcagcatatt tgttggtcag ctgagaaccc tgcgcctgca
    32221 ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc gatgtgctct tcctgacgta
    32281 ctttttcctg ctccatagcc tgccacagtt cggcatcata atcggcaatg ttcatttcac
    32341 gctttaacat ccgcatctcc tgactcagct aacaataaaa tttttggcct ttataggcgg
    32401 tcctgttgga caacggcgaa cagtataacc gaatcattgt gcgataacag gtcttgacaa
    32461 aggaatttac gcaaacgatt accttcaggc tacgcaaggc tttggagaat aaagagcttg
    32521 caaccggaaa cggatttctt ttcaggtttg tgatgcaaat ttttcacttc atcacattct
    32581 ttctgaaaaa caccaaagaa ccatttacat tgcagggcta ttttttataa gatgcatttg
    32641 agatacatca attaagatgc aaaaaaggaa gaccatatgc ttgacgctca aaccatcgct
    32701 acagtaaaag ccaccatccc tttactggtg gaaacggggc caaagttaac cgcccatttc
    32761 tacgaccgta tgtttactca taacccagaa ctcaaagaaa tttttaacat gagtaaccag
    32821 cgtaatggcg atcaacgtga agccctgttt aacgctattg ccgcctacgc cagtaatatt
    32881 gaaaacctgc ctgcgctgct gccagcggta gaaaaaatcg cgcagaagca caccagcttc
    32941 cagatcaaac cggaacagta caacatcgtc ggtgaacacc tgttggcaac gctggacgaa
    33001 atgttcagcc cggggcagga agtgctggac gcgtggggta aagcctatgg tgtactggct
    33061 aatgtattta tcaatcgcga ggcggaaatc tataacgaaa acgccagcaa agccggtggt
    33121 tgggaaggta ctcgcgattt ccgcattgtg gctaaaacac cgcgcagcgc gcttatcacc
    33181 agcttcgaac tggagccggt cgacggtggc gcagtggcag aataccgtcc ggggcaatat
    33241 ctcggcgtct ggctgaagcc ggaaggtttc ccgcatcagg aaattcgtca gtactctttg
    33301 actcgcaaac cggatggcaa aggctatcgt atcgcggtga aacgcgaaga gggtgggcag
    33361 gtatccaact ggttgcacaa tcacgccaat gttggcgatg tcgtgaaact ggtcgctccg
    33421 gcaggtgatt tctttatggc tgtcgcagat gacacaccag tgacgttaat ctctaccggt
    33481 gttggtcaaa cgccaatgct ggcaatgctc gacacgctgg caaaagcagg ccacacagca
    33541 caagtgaact ggttccatgc ggcagaaaat ggcgaagttc acgcctttgc tgatgaagtt
    33601 aaggaactgg ggcagtcact gccgcgcttt accgcgcaca cctggtatcg tcagccgagc
    33661 gaagccgatc gcgctaaagg tcagtttgat agcgaaggtc tgatggattt gagcaaactg
    33721 gaaggtgcgt tcagcgatcc gacaatgcag ttctatctct gcggcccggt tggcttcatg
    33781 cagtttaccg cgaaacagtt agtggatctg ggcgtgaagc aggaaaacat tcattacgaa
    33841 tgctttggcc cgcataaggt gctgtaattt gatgttgccg gatggaaaca tccggcaacc
    33901 cttgacgcgg tttaaattgc cgcgtcgtcc tcttcaccgg tacggatgcg aatgacccga
    33961 gccacgtcaa agacgaagat tttaccgtca ccgattttgc cggtttgcgc cgtgcgaata
    34021 atggtatcga cacaggtatc gacaatgtcg tccggtacga caatctcaat tttcactttc
    34081 ggcagaaaat ccaccatata ctccgcgccg cggtacagct cggtatggcc tttctggcga
    34141 ccaaagcctt tcacttcggt caccgtcatg ccggtaatac cgacttcggc cagtgcttcg
    34201 cggacatcgt ccagcttgaa gggttttata atcgcatcaa tctttttcat gctattcctt
    34261 gaaaaggttg cctgtctttt gatctgctaa acgtaacaca taacgccaat tcattccttg
    34321 aaatcgtttg catccagctc gtgtcgggaa agcagtttat aaaattctgt ccggttgcgc
    34381 cccgccattc tagccgcgtg ggtgacgttg cctttggtga tttgcagcag cttacgcaaa
    34441 tagttgagtt caaaatgatt acgtgcctca acaaaggttg gcagcgccgt attttcaccc
    34501 tccagcgcct gctccaccag cgcatcacta atcaccggag atgaggtcag cgccacgcac
    34561 tgttcaatca cgttgaccaa ctggcgcaca ttacccggcc agctcgcggt catcaggcgt
    34621 ttcatcgcat cggtagagaa cgcgcggaca aacggtttat gtcgctctgc cgcctggcgc
    34681 aacaggtgat ttgccagtag cggaatgtct tctgtgcgct ccgccagtgc cggaattttc
    34741 aggctgacaa cgttgaggcg gtaatagaga tcttcgcgga attccccgcg cgccatcgct
    34801 tttgacagat cacggtgagt ggcagaaata atccgcacat tgatatcaat atcgcggtta
    34861 ctgcccagcg ggcgcacttt acgctcctgc aacacgcgca gcagtttgac ctgtaacggc
    34921 gcaggcatat cgccaatttc atcgagaaat agcgttccgc cttccgccgc ctggaataaa
    34981 ccttcgcgat tgctgacagc gccagtaaac gcgccacgcg catgaccaaa cagctccgac
    35041 tccagcaatt gctcgggcaa tgcgccacag ttaatagcaa taaatggttt gctgttgcgc
    35101 gggctggcgt tgtggatagc ctgggcgaaa atctctttcc cggtgccgct ctgaccgtta
    35161 atcaaaacgc tgacgtctga ttgcgccacc agccgcgcct gttccagcaa acgcagcatc
    35221 agcgggctgc gggtgacaat tgcctcgcgc cagcgttcat cggtggctgg cgcggattgc
    35281 tccagcgcat cgtcaattgc ctgatatagc gcatctttat cgacaggctt ggtgaggaaa
    35341 ctaaaaacgc cctgctgtgt tgcagcaacg gcatcgggga tagaaccatg cgcagtaaga
    35401 ataattactg gcattcccgg ctgcactttc tggatttcag caaatagctg cataccgtcc
    35461 atttcatcca tccgcaggtc gctgataact aaatctactt tttcgcgatt cagtacccgt
    35521 aatccttcag cgccactttc cgccgtgacc acactgtagc cttcgctggt caggcgcagg
    35581 ccaagcagtt tcagcaatcc cggatcgtca tcgaccaata ataaatgcgc aggtttatgg
    35641 ctcatcagga gtgacctcat gggtggatgg cgcgggcttt tcactctcgt gcggcgtatc
    35701 cggcgagaaa tttccagccg gtttgcgggt cgagagctgg cgttcaatat cggtcaggtt
    35761 ttccagcttg cgggtggtga gttccagttg ctgctgtaga acgtgatgtt gctggcgcaa
    35821 tgtatccagc tcgctgtcgc tggactgctg gagtttactg taacgttggc gctcttccgc
    35881 cagttgcagt tgcagcgcct gaccgtcgcg ccagagttga tacagtgggc gaacctgtgc
    35941 cgggatctcg gtacttaacg cttcaatacg cgcgaccagc tggcggcgct catacggcgt
    36001 aattttggcg tcggcgagca aaatcccttg tttaaaggta ttttgccagc tgccgtcgtc
    36061 atattggcga gcttgctgac gcgactgcgc aggcattaaa cgatcagcac aatccatcgc
    36121 ccgcagccag taaagcggat tggtttcggt tgatttacct tgcagcgccc agatgtcgct
    36181 acattcagta gaaagatagt cagccagttg ataaaccgga attttttctt ctgctggcgt
    36241 atcaatggct ggcttattgt gattctgcac gcaacccagc aatgccagac atggcagccc
    36301 tgccagccac agccgtcggg gcaataatcg ttgaaaaatg tgtcgcatat tcaccagact
    36361 taaagcctat cccagtgggc gtaattgttg cagacagtct ggacatggac agcgcggaga
    36421 aaccggggcg tacatatcgt acgtgaagat ttcgagcact acccggggcc gaactgacaa
    36481 ataaaatagc ctgataggat aggctgttag cattatttcg tgtttttcga cgacggtaat
    36541 tcaatgcgga aacaaacgtc ttgcccgctc tcgtcgacca gatacagttc cccttgcata
    36601 cggcgaatac aatccctggc aatgcttaat cccagaccgc tgcccttcac cgcccctttt
    36661 cgctggtggc ttccctgaaa aaaggggtcg aagatcatgg cgcgttcctc ttgcggaatg
    36721 ggcgtgcctg tattgatgac atcaatataa acccgcgcac catgtaaact gctgcgaagg
    36781 caaatgttac cggattcagc cccgtagtgc accgcattgg agtaaagatt atccagtacg
    36841 ctcatcagca gcattggctc cgccaggcaa gctgttgctt tgagatcgac gtcggtatgc
    36901 atcattttag cccgtgcggg caggctatga gcagaaacca ctgtctccac cagcggtgct
    36961 aattcaacat tctccagttc caccgcactg tccgcctgtt tacggttgta atcaagcagt
    37021 tgttcgatca gtttttgcaa attgcagctg ctgctatcaa gaatgctcac cacctctttt
    37081 tgctctggcg taagcggccc gacaacctga tcagccagta attcagtgcc ctcgcgcata
    37141 ctcgccagtg gcgtttttaa ttcatgagat aaatgtctta aaaattgatg gcgttgggat
    37201 tccagccatg acaggcgctc acttaaccaa agaatacgtt gcccaaccga gcgtaactcg
    37261 ctcggtccac agaacgaaac gctattgccc agagaacgcc cttcccccag acggttgatc
    37321 atgcgctcga tatttttcac cggcccgata atcatccgcg tgaaaagtag caccatcacc
    37381 agactcacca gaaatagcac cagcgattgc caaccaaaat attgcccacg ttcggcgatt
    37441 tcacgctgaa gttgctgccc acgagagaac accactgtgc gcgtggcctg caccatttcg
    37501 gtattggcac tggcaaaggc ttcaagacgc gcggcggcgg cagcatcggg accactgttg
    37561 ttacactgaa gttgggccag attgttcaaa tcctgacgca atgcctggta aagtttatcg
    37621 tctggcagta cgcctgcgtg ggcatcgagc atttcgctgt aacgcttgcg ctggctttga
    37681 taaaccttca caagcgtagg gtcgtccagc acgcaatact gacggtagct acgctccatc
    37741 tccagcgccg cgttggtcat cgcttcacta cgccgggcat caataagcgt agtgcggtta
    37801 accagcgccg cctgatcgct aagcgcattc aggctttgcc aggcttgcca tgccagcacc
    37861 aacaggggta gtagaattag caaaaatgcc agcattacca gttgtcgtaa tgagcgggga
    37921 aaaacgggcc agcgtttcaa ggtgttactc tcgtcagacg cgaatagcct gatgctaacc
    37981 gaggggaagt tcagatacaa caaagccggg aattacccgg ctttgttatg gaataaggcg
    38041 gtgcctaact cgacgtttcg cccgatggtt gatatagcta cgctgatatc agaagttgga
    38101 cggcaggcac cttgttgtgc gtcattcgaa ttttatgtag cacgtcccga aggggctgac
    38161 ataagtcggt gaatgagcca ctgcttacta ttatgcagta actgtgccaa taaagaaaat
    38221 agtttggtaa cgtactgatt atacgttgct ttgagggatt tatggcttct tcgctatttg
    38281 aatgtaaatc agccactgtg tcgctaaaaa gagacaactt aagataaact tattagatta
    38341 caataaaatc aatcatttaa gtgtcgccag aaagcgacac ggtaaaccac ccattgtcgt
    38401 gatttacaga cacaaaaaag ctcccggagt tgggagctta tgatagtggt tggtgcttaa
    38461 tgccggatgc ggcgtaaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc
    38521 ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt
    38581 ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg
    38641 tgactcgacc actttcagtc gtaaccgccg taataccgtt cggtgaaccg ttcgggttag
    38701 ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt
    38761 tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag
    38821 agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac gggctttggg
    38881 ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg
    38941 gccacaactc actccccggg atcagttcac gcaggttaga catcatctgg caaccgttac
    39001 atacccccag ggacagcgtt tgcggacgat ggaagaaggt tgcaaactca tcgcgtacac
    39061 ggtcattgaa caggatagac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg
    39121 agaaacctcc gcacgcgacc agggcgtgaa aatcctccag tcccgtacgt ccagtcagca
    39181 ggtcgctcat atgcacgtcg atagcatcaa agcccccacg ttggaaagct gccgccattt
    39241 caacatgcga gttcacgccc tgctcacgca gtacggcaac tttcggacgt gcgccagtgg
    39301 caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca ttcaggcccg
    39361 gatcggcgtc gttagatttc gcctgatgct cctgatcggc acactccggg ttgtcacgca
    39421 ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtagtg cggctttcgc
    39481 tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccaa
    39541 cataatggac gcaatccgca agcccatgct gtgccagtac ggactcgacc gcttcacggt
    39601 cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat
    39661 cgtcacccag agaggcgata tccgcgtcaa tgccacaatg acctgcaaag gccatttccg
    39721 ccagcgttac cagcaggcct ccatcagagc ggtcgtgata cgccagcagc ttacgctgtg
    39781 caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg gacaacatcg cgtacatctg
    39841 ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat
    39901 tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtacaaagc tgcggcgtga
    39961 tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg
    40021 tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac
    40081 ccaccgggat cgtcaggccc agcgccggac aaagttcttc acccacggct ttaacggctt
    40141 catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt
    40201 taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg
    40261 ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca
    40321 tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca
    40381 cctgccacgg ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg cggtcaccga
    40441 tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt
    40501 ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat
    40561 cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat
    40621 tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg
    40681 cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa
    40741 ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg
    40801 gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgtccgccg tcgctcacca
    40861 gctccggcat cgcgttagaa agaccgcccg caccgacgtc gtggataaac agaattgggt
    40921 tagcatcacc aagctgccag caacggtcga tcacttcctg gcaacgacgc tccatctccg
    40981 ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacca gacgccatgg
    41041 aagacgccgc gccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct
    41101 tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc
    41161 ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccattgtgg ctgttcactt
    41221 tttcttcata agtacggaag tagccgttca gtgccggacg accaaattcg ttgttaaacg
    41281 ccgcaccgcc cagcgggcct tcggtcatga tgtccagcgc agtaacaatg cgctcaggct
    41341 taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg gatacggaga
    41401 aaccaaccaa accagctttc ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt
    41461 caccgccgga accggtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag
    41521 tttcgacttt catcaggatg tgcgccggtt cctgatggaa attgtagcgg cccgtttcgt
    41581 ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag
    41641 cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcctg aacagcgatt
    41701 tcggctgttg ttcaccgtcg ataatccagt cggcgttaaa aattttgtgg cggcagtgct
    41761 cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg
    41821 tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaagcca agacgcaggt
    41881 tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgcttgtg accggagtcg
    41941 gttgatggtg ggcaaacaac tgctctgcat catctaaatc aaaaaagacc gtttccatca
    42001 tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt
    42061 ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg
    42121 caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca
    42181 ggagtttgcc ttgcggggcg tggctggcga gtgccggacc atatttcagc aggcgttcaa
    42241 gttgtgcgtg ctcatcatcg tttaacgacg cattgaggtc agcaaaatgg acatactcgg
    42301 cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc
    42361 ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta
    42421 agctttcggt gtacccaagg ggggggaaac gggcgtcatt ataaagaatc tgatgcgctg
    42481 acgaaaccgt ttgcgtggaa ataaaatcac catcgtgaat tagcaacgcg tgccgccaat
    42541 ggctgtaata agttgccatc tggcgcaggt ttacgcaaaa tgccgctcat ttatgagtaa
    42601 acctttcact attattacgt tttttcaagc tgggacgcgc acgacacaga gaattaacta
    42661 attgaaaaaa ttaaagatta attatctgtt catcggcatt ctggcactgc tgctcgcggt
    42721 cgctctctgg ccatccattc cctggtttgg taaagccgac aaccgtatcg ccgccattca
    42781 agcgcgggga gagttgcgtg tgagcaccat tcatactccc ctgacttata acgaaatcaa
    42841 cgggaaacct tttggcctag attacgaact ggcgaaacag tttgccgatt acctcggcgt
    42901 aaaactgaaa gtgaccgtgc ggcagaatat cagccagctg tttgacgacc ttgataatgg
    42961 taacgccgac ctgctggcgg caggactggt ctataacagt gagcgggtaa aaaattatca
    43021 gcctggccct acctattatt ccgtgtcaca acaactggtt tataaagtgg gtcagtatcg
    43081 cccacgtacg ctgggcaacc tgacggcgga gcaactcacc gttgcaccgg gtcatgtggt
    43141 ggttaacgat ctccagaccc tgaaagaaac aaaattcccg gaattaagct ggaaggtaga
    43201 cgacaaaaaa ggctctgcgg aattaatgga agatgtcatc gaaggaaaac tcgattacac
    43261 cattgctgat tctgtcgcca tcagcctgtt tcagcgcgtt cacccggagc tcgccgtagc
    43321 gctcgatatc accgatgaac aaccggtgac ttggtttagc ccgttagatg gcgataatac
    43381 cctttccgcc gccctgctcg acttcttcaa cgaaatgaat gaagacggta cgctggcacg
    43441 cattgaagag aaatacctgg ggcatggcga tgattttgat tacgtcgata cgcgcacatt
    43501 tttacgcgcc gtcgatgcgg tactgccgca gttaaagccc ctgtttgaga aatacgccga
    43561 agaaattgac tggcgtttgc tggccgctat tgcttatcag gaatcgcact gggatgcaca
    43621 ggccacttca ccgacgggtg tgcgcggcat gatgatgtta accaaaaata ccgcgcaaag
    43681 cctcggcatt acggatcgta ccgatgccga acagagcatc agcggtggcg tgcgttattt
    43741 gcaggatatg atgagtaaag tgccggaaag tgtgccggag aacgaacgga tctggtttgc
    43801 cctcgctgcg tacaatatgg gctatgcgca tatgctggat gcccgcgccc tgacggcaaa
    43861 aaccaaaggg aatcctgaca gttgggctga cgtaaaacag cgtctgcctt tacttagcca
    43921 gaaaccctat tacagcaagc tgacttacgg ctacgctcgt gggcatgaag cctacgctta
    43981 tgtcgaaaat attcgtaagt atcagattag cctggtgggt tatctgcaag agaaagagaa
    44041 gcaggctaca gaagcggcga tgcaactggc gcaggattat ccggcggtat cgcctacgga
    44101 gttgggcaaa gagaaatttc cttttctctc gtttctttcc cagtcgtcat caaactattt
    44161 gacccattct ccctctctgc tgttttccag gaaagggagt gaagagaaac aaaattaatc
    44221 cgtcgaggat tgcgcttttt tctgcgcttt aatttcctgg cggcgcatgc gaaagaagtc
    44281 actgagcaac gccgcgcact catccgccag tattccttcc gtaatttcca ctcggtgatt
    44341 catacccgga tgatgcagca catccattaa agatcccaca gcgccagttt tcgcgtcacg
    44401 cgcaccaaag accacgcgac caatgcgact gtggatcatc gctccggcac acattacaca
    44461 tggttcaagc gtgacataca acgtggcgtc gatcagacga taattttgca tcaccagacc
    44521 accctgccgc agggccatga tttctgcatg tgcggtggga tcatggcgac caatcgggcg
    44581 gttccagcct tcgccgatta cccgattgtt atgcactaat accgcgccga ccggcacttc
    44641 ccgctcatcc caggcacgtt tcgccagcgt cagcgcgtga cgcatccaat attcgtggct
    44701 aaattcgact tcagacaaaa agaaaactcc ggttataaaa gcgcggcgca ttatacacgg
    44761 acgctatgct ttactctatt ccagttgctg gagttcaccg cgcggggtta ctcgccaacg
    44821 atgctgacag aaataaagca gcggattgtc ctgattactg tcgctatagc cactgtacag
    44881 ccgcagcggc gtgccgattt tgcgctccag ttgtgcgacc ttttcatgtc ccagacaacg
    44941 catcgtcaat acccaaccac catagccacg ctgaatttgg ctggcgataa gattaacccg
    45001 cggcagccag ggcgtatcga aataaaccgc ttcaaccagc ggctgcggag agccggtaat
    45061 caaccagata tcagcatcgg aacttaacag gtaggtggtt aatcgctcct gaaccagcgg
    45121 aaaggcggta acattgtcgc gaaaccagcg cacgaaatcg gcctgcaacg tctgtaaacg
    45181 tgcttcgctg tgaccaaaag tgcaccccca cagaagcaga ctcatcggcc agcgtgccgc
    45241 acgacctttt atcaataacg caatggctat aatcggtaac aacggcagga caagtaacgc
    45301 attcagcggt tggcgacgca gtaaatagcg cagaaaactg ccgaacatat cctgctgatg
    45361 caatgttcca tctaagtcaa aaaacaccac acgacgctcg tgagttgcca aaccatactc
    45421 ctctggactg aaacatcctg attaattttc gctactacat agcctaacag atagatcatc
    45481 acttttccgg caacaatcga ccgcatcatg gctaactgga atttttaatt catggcaatt
    45541 agcggcaatg gaatataaaa ttcactcgcg tgtgtctcat attgcctgtt gtcgccacta
    45601 tttcatcgcg aggaaagata tgaacggctt acttcgtatc cgtcagcgtt accaagggct
    45661 tgcccagagt gataaaaaac tggcggatta tctgctgcta caacctgata cggcgcgcca
    45721 tttaagctct cagcaactgg ccaacgaagc cggagtcagt cagtccagcg tcgtgaagtt
    45781 cgcgcaaaaa ctcggctata aaggttttcc ggcgcttaag ttggcgttga gtgaagcgct
    45841 ggcaagccag ccggaatcgc cctccgtgcc cattcataac caaatccgcg gtgatgatcc
    45901 gttacggctg gtcggcgaaa aactgattaa agaaaatact gccgctatgt acgcaacgct
    45961 aaacgttaat agtgaagaga aactgcatga atgcgtaaca atgttgcgct ctgcgcggcg
    46021 gataattctg accggtattg gcgcttcggg tctggtggcg caaaactttg cctggaagct
    46081 gatgaagatt ggcttcaatg ctgccgcact gcgcgatatg catgcgctac tcgcaacagt
    46141 acaggcgtcg tcccctgacg atctgttatt agccatttcc tacaccggtg tacgacgcga
    46201 gttaaacctg gcggcagatg agatgctgcg agtgggcgga aaagtgctgg cgattaccgg
    46261 ctttactccg aatgccctgc aacagcgtgc ttctcattgc ctgtatacca ttgccgaaga
    46321 acaagcgaca aacagtgctt caatctctgc ttgtcacgct cagggaatgt taacggattt
    46381 gctgttcatt gcgctgattc agcaggatct ggaactggca ccagaacgta ttcgtcatag
    46441 tgaagcgctg gtgaaaaaac tggtctgagt aaagaatgcg cgtataatgc ccgcccggtt
    46501 tgtgttgttt tgagagtttc cttatggcgc tgttaatcac taaaaaatgc atcaattgtg
    46561 atatgtgtga aatcaaataa aaacacattt attttcatag tgatacagca atgtgttatt
    46621 gaatgtgttg ttggatttat ggtgcaaaaa aattttgcat aagttcagca acacaaagaa
    46681 caccactttc tttaacgtcc tccgcgccct aaaggacggg gctttacggc gcaaaaggcc
    46741 tgtgacatac aacattaact tttcagtcaa ccgtagcacg ttcttgtata cgccgtgtct
    46801 gcggttttat ataaccctgg ctggaaacct cttatacaaa gtcgattcgc caacatcata
    46861 gatgatcgcc accttctggc gagaaactcc tgatgcaatt agtcgcccgg cctgcgccca
    46921 ttattctggt gaaagtttgg gacggcgacc gccaattcgt ccctgtgcgc gagcagcttc
    46981 cagtcctgct tttgttcgtt caacaatcag ttcacgttcc atttcagcca gggcacccat
    47041 cacatggaag aaaaagcgtc ccattggtgt gctggtatca attgaatcag tcagactacg
    47101 aaagttgatg cctcgttcgc gcaactcctc caccagcacg acaagatgcc gcatactgcg
    47161 ccccagccga tccagcttcc agacaaccag tgtgtcgcct gccgataatg tcttgagcag
    47221 ttttttcagc cccggtcttt cggactttgt accacttatc ttgtcttcaa aaatcagctc
    47281 gcatcctgca cagtt
//
LOCUS       DAEEGA010000027        46615 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEEGA010000027 DAEEGA010000000
VERSION     DAEEGA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46615
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.027"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(19..606)
                     /gene="mog"
                     /locus_tag="KLH46_001552"
     CDS             complement(19..606)
                     /gene="mog"
                     /locus_tag="KLH46_001552"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBE0378279.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(721..1674)
                     /gene="tal"
                     /locus_tag="KLH46_001553"
     CDS             complement(721..1674)
                     /gene="tal"
                     /locus_tag="KLH46_001553"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBE0378280.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            1953..3382
                     /locus_tag="KLH46_001554"
                     /pseudo
     CDS             1953..3382
                     /locus_tag="KLH46_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            3452..4228
                     /gene="yaaA"
                     /locus_tag="KLH46_001555"
     CDS             3452..4228
                     /gene="yaaA"
                     /locus_tag="KLH46_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBE0378281.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(4381..4677)
                     /locus_tag="KLH46_001556"
     CDS             complement(4381..4677)
                     /locus_tag="KLH46_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBE0378282.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPSPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(4891..6177)
                     /gene="thrC"
                     /locus_tag="KLH46_001557"
     CDS             complement(4891..6177)
                     /gene="thrC"
                     /locus_tag="KLH46_001557"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBE0378283.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(6178..7110)
                     /gene="thrB"
                     /locus_tag="KLH46_001558"
     CDS             complement(6178..7110)
                     /gene="thrB"
                     /locus_tag="KLH46_001558"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBE0378284.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(7112..9574)
                     /gene="thrA"
                     /locus_tag="KLH46_001559"
     CDS             complement(7112..9574)
                     /gene="thrA"
                     /locus_tag="KLH46_001559"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBE0378285.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(9600..9719)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(9655..9720)
                     /gene="thrL"
                     /locus_tag="KLH46_001560"
     CDS             complement(9655..9720)
                     /gene="thrL"
                     /locus_tag="KLH46_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBE0378286.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(9934..10620)
                     /locus_tag="KLH46_001561"
     CDS             complement(9934..10620)
                     /locus_tag="KLH46_001561"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBE0378287.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTALAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(11020..11160)
                     /locus_tag="KLH46_001562"
     CDS             complement(11020..11160)
                     /locus_tag="KLH46_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378288.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            11256..11972
                     /gene="arcA"
                     /locus_tag="KLH46_001563"
     CDS             11256..11972
                     /gene="arcA"
                     /locus_tag="KLH46_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBE0378289.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(12031..13383)
                     /gene="creD"
                     /locus_tag="KLH46_001564"
     CDS             complement(12031..13383)
                     /gene="creD"
                     /locus_tag="KLH46_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBE0378290.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFLLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFILALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(13441..14865)
                     /gene="creC"
                     /locus_tag="KLH46_001565"
     CDS             complement(13441..14865)
                     /gene="creC"
                     /locus_tag="KLH46_001565"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBE0378291.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(14865..15554)
                     /gene="creB"
                     /locus_tag="KLH46_001566"
     CDS             complement(14865..15554)
                     /gene="creB"
                     /locus_tag="KLH46_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBE0378292.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(15567..16040)
                     /gene="creA"
                     /locus_tag="KLH46_001567"
     CDS             complement(15567..16040)
                     /gene="creA"
                     /locus_tag="KLH46_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBE0378293.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16251..17120
                     /gene="robA"
                     /locus_tag="KLH46_001568"
     CDS             16251..17120
                     /gene="robA"
                     /locus_tag="KLH46_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBE0378294.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(17117..17764)
                     /gene="gpmB"
                     /locus_tag="KLH46_001569"
     CDS             complement(17117..17764)
                     /gene="gpmB"
                     /locus_tag="KLH46_001569"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBE0378295.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWVERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            17816..18336
                     /gene="yjjX"
                     /locus_tag="KLH46_001570"
                     /pseudo
     CDS             17816..18336
                     /gene="yjjX"
                     /locus_tag="KLH46_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(18421..18747)
                     /gene="trpR"
                     /locus_tag="KLH46_001571"
     CDS             complement(18421..18747)
                     /gene="trpR"
                     /locus_tag="KLH46_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBE0378296.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(18837..20774)
                     /gene="sltY"
                     /locus_tag="KLH46_001572"
     CDS             complement(18837..20774)
                     /gene="sltY"
                     /locus_tag="KLH46_001572"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBE0378297.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            20985..22652
                     /gene="ettA"
                     /locus_tag="KLH46_001573"
     CDS             20985..22652
                     /gene="ettA"
                     /locus_tag="KLH46_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBE0378298.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(22759..22926)
                     /locus_tag="KLH46_001574"
     CDS             complement(22759..22926)
                     /locus_tag="KLH46_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378299.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(22960..24192)
                     /gene="nadR"
                     /locus_tag="KLH46_001575"
     CDS             complement(22960..24192)
                     /gene="nadR"
                     /locus_tag="KLH46_001575"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBE0378300.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(24213..25595)
                     /gene="radA"
                     /locus_tag="KLH46_001576"
     CDS             complement(24213..25595)
                     /gene="radA"
                     /locus_tag="KLH46_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBE0378301.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSGDLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(25644..26612)
                     /gene="serB"
                     /locus_tag="KLH46_001577"
     CDS             complement(25644..26612)
                     /gene="serB"
                     /locus_tag="KLH46_001577"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBE0378302.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            26718..27362
                     /locus_tag="KLH46_001578"
     CDS             26718..27362
                     /locus_tag="KLH46_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBE0378303.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            27390..28405
                     /gene="lplA"
                     /locus_tag="KLH46_001579"
                     /pseudo
     CDS             27390..28405
                     /gene="lplA"
                     /locus_tag="KLH46_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            28437..28700
                     /locus_tag="KLH46_001580"
     CDS             28437..28700
                     /locus_tag="KLH46_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0378304.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(28861..29580)
                     /gene="deoD"
                     /locus_tag="KLH46_001581"
     CDS             complement(28861..29580)
                     /gene="deoD"
                     /locus_tag="KLH46_001581"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBE0378305.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(29637..30860)
                     /gene="deoB"
                     /locus_tag="KLH46_001582"
     CDS             complement(29637..30860)
                     /gene="deoB"
                     /locus_tag="KLH46_001582"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBE0378306.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(30912..32234)
                     /gene="deoA"
                     /locus_tag="KLH46_001583"
     CDS             complement(30912..32234)
                     /gene="deoA"
                     /locus_tag="KLH46_001583"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBE0378307.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(32361..33140)
                     /gene="deoC"
                     /locus_tag="KLH46_001584"
     CDS             complement(32361..33140)
                     /gene="deoC"
                     /locus_tag="KLH46_001584"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBE0378308.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            33398..34939
                     /locus_tag="KLH46_001585"
     CDS             33398..34939
                     /locus_tag="KLH46_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBE0378309.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            34911..35774
                     /locus_tag="KLH46_001586"
     CDS             34911..35774
                     /locus_tag="KLH46_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBE0378310.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(35988..36770)
                     /locus_tag="KLH46_001587"
     CDS             complement(35988..36770)
                     /locus_tag="KLH46_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBE0378311.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(36767..37840)
                     /locus_tag="KLH46_001588"
     CDS             complement(36767..37840)
                     /locus_tag="KLH46_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBE0378312.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYEPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(37962..38123)
                     /locus_tag="KLH46_001589"
     CDS             complement(37962..38123)
                     /locus_tag="KLH46_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBE0378313.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(38240..38845)
                     /gene="osmY"
                     /locus_tag="KLH46_001590"
     CDS             complement(38240..38845)
                     /gene="osmY"
                     /locus_tag="KLH46_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBE0378314.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(39238..40827)
                     /gene="prfC"
                     /locus_tag="KLH46_001591"
     CDS             complement(39238..40827)
                     /gene="prfC"
                     /locus_tag="KLH46_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBE0378315.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(40918..41595)
                     /gene="yjjG"
                     /locus_tag="KLH46_001592"
     CDS             complement(40918..41595)
                     /gene="yjjG"
                     /locus_tag="KLH46_001592"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBE0378316.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(41610..42056)
                     /gene="rimI"
                     /locus_tag="KLH46_001593"
     CDS             complement(41610..42056)
                     /gene="rimI"
                     /locus_tag="KLH46_001593"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBE0378317.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHSFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(42025..42438)
                     /gene="holD"
                     /locus_tag="KLH46_001594"
     CDS             complement(42025..42438)
                     /gene="holD"
                     /locus_tag="KLH46_001594"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBE0378318.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            42541..43572
                     /gene="rsmC"
                     /locus_tag="KLH46_001595"
     CDS             42541..43572
                     /gene="rsmC"
                     /locus_tag="KLH46_001595"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBE0378319.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFAHHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            43849..43935
                     /locus_tag="KLH46_001596"
     tRNA            43849..43935
                     /locus_tag="KLH46_001596"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43883..43885,aa:Leu,seq:cag)
     gene            43964..44050
                     /locus_tag="KLH46_001597"
     tRNA            43964..44050
                     /locus_tag="KLH46_001597"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43998..44000,aa:Leu,seq:cag)
     gene            44085..44171
                     /locus_tag="KLH46_001598"
     tRNA            44085..44171
                     /locus_tag="KLH46_001598"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44119..44121,aa:Leu,seq:cag)
     gene            complement(44210..44446)
                     /locus_tag="KLH46_001599"
     CDS             complement(44210..44446)
                     /locus_tag="KLH46_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBE0378320.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            44587..45375
                     /gene="fhuF"
                     /locus_tag="KLH46_001600"
     CDS             44587..45375
                     /gene="fhuF"
                     /locus_tag="KLH46_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBE0378321.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(45413..46090)
                     /gene="bglJ"
                     /locus_tag="KLH46_001601"
     CDS             complement(45413..46090)
                     /gene="bglJ"
                     /locus_tag="KLH46_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBE0378322.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLSRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(46048..>46615)
                     /locus_tag="KLH46_001602"
     CDS             complement(46048..>46615)
                     /locus_tag="KLH46_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE0378323.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        11047 a        12700 c        11728 g        11140 t
ORIGIN      
        1 ccgctcgggg ggattttttt attcgctaac gtcgcgtctt gcactcttcg gtctgaatgc
       61 agcaaccact tctggtgccg tttcaacgta tggcccttcc agcaactgaa tgcagtacgg
      121 tacgctggca aaaataccat gcaccacaac gttaccctca gcgtccttca caccttccag
      181 cgtctcttta atagacttcg gctgaccggg taagttaagg atcagcgcct gtttgcgaat
      241 cacgcccacc tgacgcgaaa ggatcgcagt tggtacaaaa tgcaggctga tctggcgcat
      301 ctgttcacca aagccaggca tctcgcggtc cgctactgcc agcgtcgcat cgggcgttac
      361 gtcacgacgc gccgggccag ttccgcccgt ggtgagcacc agatggcaac tcatttcatc
      421 caccagctca cacaacgttt gctcgatgat cgcctgctca tcggggatta agcgggtttc
      481 cagttcaaac ggcgtggtta gcgccgatgt cagccattct tccagcgctg gaatgccttt
      541 atcctgataa acgccgctgg atgcgcgatc agagatggaa actaagccaa tacgtaaagt
      601 attcatcaag atttttccgt ttaaacaatc tcgttaaaca gaatgataca ctgcgaaggg
      661 agtgacagac aggcttcaga agaggtagcg tgaccgactt cccggtcacg ctaagaatga
      721 ttacagcaga tcgccgatca ttttttccag tttttcctgg tcaatagcaa acttacggat
      781 accttccgcc agtttatcta ctgccattgg atcctggttg tgctgccaca ggaactcgga
      841 ctcagtgata cgcgccggac gcgctttcac ttcgccggtg taagacagtt tacgttcgat
      901 agccccttcg ctctccgcca gctctttcag cagtgccggt gcgatggtca gacggtcaca
      961 gcctgccagt tccagaattt cgccgatgtt acggaagctt gcgcccataa ccacggtttc
     1021 ataaccgtgc tctttgtagt actggtagat ttcagataca gaaaccacgc ccggatcttc
     1081 tgccggagcg tactctttct tatcggtatt cgctttgtac cagtcaagaa tacggccaac
     1141 aaacggcgag atcaggaaca cgcccgcctc cgcacaagca cgagcctgag cgaaggagaa
     1201 cagcagggtc aggttacagt tgataccttc tttttccagc tgttctgcag cacggatacc
     1261 ctgccaggta gaagccagtt tgatcagaat acgatcgttg ctaatacctg catcgttgta
     1321 gagtttgatc aggcgttttg ctttcgcaat tgacgcttcg gtgtcatagg aaagacgcgc
     1381 atcaacttca gttgagatac ggcccggaac cagtttcagg atttccagac caatatttac
     1441 tgccagtttg tcggtcgcgt ccacgatctg ctgcgcgcga tcgttgctct gctgtttcgc
     1501 ccaggcgaca gcatcatcaa tcaacttacg gtattccgga atctgcgctg cgttaagaat
     1561 gagagaaggg ttggttgtgg catcctgcgg ttgatacagc ttcattgccg cgatgtcccc
     1621 agtgtcggcc actacggtgg tgtactgacg aagggaggtc aatttgtccg tcatgatagt
     1681 atttctcttt aaacagcttg ttagggggat gtaaccggtc tgccctgatg atatcacgac
     1741 gcaacgtcag cgcaaccgcc gacaagacgg ttatcgccag gcgagactgt ttcggatttc
     1801 tgacactgcg ttgatatcgc ccaccatttt tatacaaaac ctcatgtatg ctacgcagaa
     1861 gttatcaagt acctcgtagc gtatatactt cttaaacaat tggtaacgtt tacacaggaa
     1921 agtcatcgcg accggcaata agagggatat gcatgccaga ttttttctcc tttattaaca
     1981 gcgtcctttg gggatcggta atgatttacc tgctcttcgg cgcaggttgt tggttcactt
     2041 ttcgcaccgg atttgtgcag tttcgctaca tccgccagtt tggcaaaagt cttaaaaata
     2101 gcattcatcc acagccaggc ggtttaacct catttcagtc attgtgtacc agtcttgcgg
     2161 cgcgcgtggg tagcggcaac ctggccggcg ttgcgctggc aattaccgcc ggtggaccag
     2221 gtgccgtctt ttggatgtgg gttgctgcgt ttatcggcat ggcgacctcg tttgccgaat
     2281 gttcccttgc acaactttat aaagaacgtg acgtcaatgg gcagtttcgt ggcggaccgg
     2341 catggtatat ggcgcgcggg ctggggatgc gctggatggg cgttctgttc gccgtctttt
     2401 tgctcatcgc ctacggcata attttcagcg gagttcaggc gaacgccgtt gcccgagccc
     2461 tgagtttttc ttttgatttt cctccgctgg tgacgggcat tattctcgct gtctttgctc
     2521 tgctggcaat cactcgcggt cttcatggcg tcgcccggct catgcagggg tttgtcccgt
     2581 tgatggcgat aatctgggta ctgaccagcc tggtgatttg cgtaataaat atcgggcaac
     2641 ttccccacgt catttggtct atttttgaga gtgcttttgg ctggcaggaa gcggcaggcg
     2701 gcgcggcggg atatacctta agccaggcga ttactaacgg ttttcagcgc agtatgtttt
     2761 ccaatgaggt gggaatggga tcgacgccaa acgcggcagc ggcagctgca tcctggcctc
     2821 cgcatccggc agcacaaggg attgtccaga tgattggcat ttttatcgac accctggtca
     2881 tctgtacggc aagcgccatg ctgatattac tggcgggtaa cggcacaacc tacatgccgc
     2941 tggaaggtat tcagcttatc cagaaggcga tgcgggtgct aatgggttcc tagggtgctg
     3001 aatttgttac cctcgtggtt attctgtttg ccttcagctc catcgttgcc aactacattt
     3061 atgccgaaaa caatctcttc tttttacgcc tgaacaaccc taaagcgatc tggtgtttgc
     3121 ggatctgcac cttcgcaacg atcatcggcg gcaccttgct aagccttccg ctgatgtggc
     3181 aactggcaga tatataatgg cctgcatggc tattaccaat ttgaccgcca ttttactgct
     3241 ctcgcctgtg gttcatacca ttgccagtga ttatctacgc cagcgtaaac tcggcgtgcg
     3301 cccggtgttt gatccgttgc gttatccgga tatcggtcgc caactttctc cggacgcgtg
     3361 ggatgatgtt tcgcaggagt aatcacaact atcgatcaac tcattctctt tttttgctaa
     3421 agtcggcata aatttcctgc aaggactgga tatgctgatt cttatttcac ctgcgaaaac
     3481 gcttgattac caaagcccgt tgaccaccac gcgctatacg ctgccggagc tgttagacaa
     3541 ttcccagcag ttgatccatg aagcgcggaa actgacgcct ccgcagatta gcacgctgat
     3601 gcgcatcagc gacaaactgg cgggtatcaa cgccgctcgc tttcatgact ggcagccaga
     3661 tttcacgccg gcgaatgccc gccaggcgat tctggcgttt aaaggtgatg tctacaccgg
     3721 cttgcaggcc gagaccttca gcgaagacga tttcgatttt gcccaacagc atttgcgaat
     3781 gctttccggc ttgtatggcg tactgcgccc gctcgattta atgcagcctt atcgtctgga
     3841 aatggggatc cgtcttgaga atgcccgagg gaaagatctg tatcaattct ggggagatat
     3901 catcaccaac aagctgaacg aggcgctcgc agcacaaggc gataatgtgg tgattaatct
     3961 ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg agattatcaa
     4021 gccggtgttc ctcgatgaga agaacggcaa atttaagatc atcagcttct acgctaagaa
     4081 agcacgcggt ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac cagagcaact
     4141 gactggtttt aatagcgaag gttacttctt tgatgaagat tcctccagca atggcgaact
     4201 ggtgtttaaa cgctacgagc agcgttaagt ttgtagtcaa tgccggatgc ggcgtgaacg
     4261 ccttatccgg cctacaaaag catgcagatt caatatattg cagaattaac gtaggcctga
     4321 taagacgcgc cagcgtcgca tcaggcgctg aatgccggat tgttacccgg catttgtcat
     4381 ttagcggtga tgtttgcctg gaccatgacc gccgtgatga tcatgaggag ctttcttatg
     4441 gtggcgcggc ggtgacggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg
     4501 ttgtttccac cagccgtggt cgcgccagtg acctccatcc cagtaatagc cacgattatc
     4561 acgatcgcct atctgtaatt ttactgacgg gactaacgta atttccgcag cctgtgctgc
     4621 catgggagcg accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg
     4681 ttactccttt tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagaacga
     4741 cggttgttat tctccgcaat cctaatctct aagttaccgc atttattgat aatttctcct
     4801 ttttccctgt cgtttttctc cataatttct tcataaaaaa gccgggctgc ataaaagcaa
     4861 acccggcctg attgagataa tgaatagatt ttactgatga ttcatcatca atttacgcaa
     4921 cgcagaaaaa tcggcgggca gattatgtga aagcaagggt aaatcagcac gttctgccag
     4981 ctcttttggc agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc
     5041 cggatgcgcg gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc
     5101 acgatacgct acggcagcgt gcggctccga ggtgtagccc agttctttta actcacgcat
     5161 tgtctgttgc gtggtttcat catccacggc tgcataaccc agctctttca gttgccagat
     5221 tttgcggcgg aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc
     5281 gttggataac gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg
     5341 cacggtatcg ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgaatgactt
     5401 cgccagcaga cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga caaccagctg
     5461 gttgcgcgcc tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag
     5521 caaacggctg atgttaatcg agttagccga gtttaacccc agcgccactt tcagttcttc
     5581 atcatcaaac gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac
     5641 agtttcgata ttgccgccca acgtacagaa cagtttttct tgcagtggac tgattttgcc
     5701 tcgtggatag aggataacca ctttcacatt cggcaaaccg tagaaagcat gagccactgc
     5761 agctccggta tcaccggagg tcgcggtcag aatggtcact ggcttatcat ccgcaatatg
     5821 ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg
     5881 gaacaattcc agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa
     5941 cgccgcgcgc acgcgctctt ccaggatttc ctgtgggatt tcatcaccaa taaacgccga
     6001 gaggatcttc gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag
     6061 gctgaattcc ggcaggtcgt gcggaaaaaa cagcccctga tttttgccca acccctgggt
     6121 tacggcttgc gcaaagctga cctgctcgtt gtgatctttc agattgtaga gtttcattta
     6181 gttttccagt actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg
     6241 attttgcagg tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc
     6301 acatagagcg aacaaggtcg ggccggagcc ggagataccg ctcactaccg cgccgatttc
     6361 cgcgaccgcc tgccgcgcct gccggaagcc aggcagtaac cgttcacggt agggttcagc
     6421 gataacatct ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat
     6481 gaagccagcc agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa
     6541 aatagcccgg gcttctgccg tcgagacttt aattcccgga tacgccagca cccacagcca
     6601 ctcatcaaac cctggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat
     6661 accaccaaga aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga tacgtccttc
     6721 cagctcgccc atcaaagcca gcaaacgagt gtcattaagc ggcttgccgc agtgttcatt
     6781 catcgccatc agcgccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgatcgg
     6841 catattcttt tccagagtca tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg
     6901 ctcccagcac tgataaacga tattttcccg tggttctgac ggcagcttat cggcaaagcg
     6961 tccgaggttg ttgagactga atgtctctgc cgcctcaacc gtgactacat ctccgagcaa
     7021 tgcaccatca acgggtgcca ccgccgcccc gagcacatca aacccgacgc tcatattggc
     7081 actggaagcc ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac
     7141 gtagcagatc ggcaaagaca ccggcagcgg taacgtcatt gcccgcacca tatccgcgca
     7201 gcaccaacgg cagcggctga taatagtggc tataaaaggc cagggcgttt tcgccatttt
     7261 tcactttgaa cagcggatca ttcccatcca cttcggcaat cttcacgcgg cagacaccat
     7321 cttcatcaat attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc
     7381 gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat aaaagcggca acatcaccct
     7441 cggcgttaaa ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac
     7501 gtcccgtttc acgagcgaga atcaatagtt tacgcgccac atccatacca gaaagatcat
     7561 ctcgcgggtc cggttcggta taacccattt cccgcgccag cgtggtcgcc tcggagaaac
     7621 tcatgccttc gtctaacttg ccgaagatat aagaaagcga acctgaaaga atgccggaga
     7681 acttcaccaa ttcatcacct gcattgagca gattttgcag gttctcaata accggtaatc
     7741 cagccccaac gttggtgtca tagaggaatt tacgccgcga tttttccgcc gcataacgca
     7801 actgatgata gtaatccatc gacgaggtgt tggccttttt gttcggcgta acaacgtgga
     7861 aaccttcgcg caggaagtcg gcatattgat ccgccactgc ctggctggag gtgcagtcaa
     7921 caatgaccgg gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa
     7981 acggctcttt ggcttgtgca agttcttcct gccagttttc cagattaagg ccatgcacac
     8041 tagtgagcag agccttcgag ttggcaacgc cgcagacacg taagtcgata tgtttattct
     8101 tcagccagct ttgctgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc
     8161 caatcacaaa cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca
     8221 cgccagtggt cgcatcatcg ttatttacca cgacagagat tgagcgttca gaagatccct
     8281 gagcaatggc gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga
     8341 tcccacgcaa ggtgcgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca
     8401 ccgccagcgg ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc
     8461 gttcagctcg cacacagtcg ctttgtggaa cgcagaaact gatactgtat tcggaagatg
     8521 attgcgtaat cagcaccacg gaaatacggg cgcgtgacat tgcagcaaag acgcgcgccg
     8581 ccatgccaac catccctttc atccccgggc cggaaacgct gaacattgcc atgttattca
     8641 gattggaaat gcccttgacc ggtaattcgt cttcatcacg gctggcacca atgagcgtac
     8701 ctggtgcttg aggatttccg gtatttttaa tcaggcaagg gatctggaac tgggcgatgg
     8761 gggtaatggt gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct
     8821 ggtaggacat cgacttcaac aacctcgcat cgggcacctg acgcgggtcg caggtataga
     8881 ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg taaacaggca gccagcaccg
     8941 ccgcggagta gtcggaaccg ttacgtccaa gcaccaccag ttcgcctttt tcattaccgg
     9001 cggtgaaacc tgccatcaac accatgtgat cagccggaat gcggcttgcc gcaatacggc
     9061 gggtggactc agcaatatcg acggtagatt cgaggtaatg ccccactgcc agcagttttt
     9121 cgaccggatc gataacggta acgttgtgac cacgcgcttc taacacgccg gccataatgg
     9181 cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc
     9241 ccaacaaact aatgccatgc aggacatgtt ttatttgggc aaattcctga tcgacgaaag
     9301 ttttcaattg cgccagcggg aaccccggct gggcggcggc gagtcccgtc aaaagttcgg
     9361 caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt
     9421 caatcatcgc caccaggtgg ttggtgattt tggcgggggc agagaggacg gtggccacct
     9481 gcccctgcct ggcattgctt tccagaatat cggcaacacg cagaaaacgt tctgcatttg
     9541 ccactgatgt accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg
     9601 aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc
     9661 cgcaccgtta cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat
     9721 ggatgttgtg tactctgtaa tttttatctg tctgtgcgct atgcctatat tggttaaagt
     9781 atttagtgac ctaagtcaat aaaattttaa tttactcacg gcaggtaacc agttcagaag
     9841 ctgctatcag acactctttt tttaatccac acagagacat attgcccgtt gcagtcagaa
     9901 tgaaaagctg aaaaatactt actaaggcgt tttttatttg gtgatatttt tttcaatatc
     9961 atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca ggcgttgctg
    10021 taaccagtcg accagtttta tgtcatctgc cactgccaga gccgtcagca atgccatgac
    10081 tcgttcgcgt aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt tcgccggttg
    10141 ttgtattaat gttgctaatt gatagcaata gaccatcacc gcctgcccca gattgagcga
    10201 aggataatcc gccaccatcg gcacaccagt aagcacgtca gccaacgcta actcttcgtt
    10261 agtcagcccg gaatcttcgc gaccaaacac cagcgcggca tggctcatcc atgaagattt
    10321 ttcctctaac agcggcacca gttctactgg cgtggcgtag taatgatatt tcgcccgact
    10381 acgcgcagtg gtggcgacag tgaaatcgac atcgtgtaac gattcagcca gtgtcgggaa
    10441 aactttaata ttatcaataa tatcaccaga tccatgtgcg acccagcggg tggctggctc
    10501 caggtgtgcc tgactatcga caatccgcag ttcgctaaaa cccatcgttt tcattgcccg
    10561 cgccgctgcc ccaatatttt ctgctctggc gggtgcgacc aggataatcg ttatacgcat
    10621 attgccactc ttcttgacca aataaccgcg aacagggtga tcactgtcaa cttattacgc
    10681 ggtgcgaatt tacaaattct taacgtaagc cgcagaaaaa gccctttact tagcttaaaa
    10741 aaggctaaac tattttctga ctgcactaac ggtttaattg ttaaaaaatg ctacatatcc
    10801 ttctgtttac ttaggataat tttataaaaa ataaatctcg acaattggat tcaccacgtt
    10861 tattagttgt atgatgcaac tagttggatt attaaaataa tgtgacgaaa gctagcattt
    10921 agatacgatg atttcatcaa actgttaacg tgctacaatt gaacttgata tatgtcaacg
    10981 aagcgtagtt ttattgggtg tccggcccct cttagcctgt tatgttgctg ttaaaatggt
    11041 taggatgaca gccgtttttg acactgtcgg gtcctgaggg aaagtaccca cgaccaagct
    11101 aatgatgttg ttgacgttga tggaaagtgc atcaagaacg caattacgta ctttagtcat
    11161 gttacgccga tcatgttaat ttgcagcatg catcaggcag gtcagggact tttgtacttc
    11221 ctgtttcgat ttagttggca atttaggtag caaacatgca gaccccgcac attcttatcg
    11281 ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg gaaggctatg
    11341 atgttttcga agcgacagat ggcgcggaaa tgcatcagat cctctctgaa tatgacatca
    11401 acctggtgat catggatatc aatctgccgg gtaagaacgg tcttctgtta gcgcgtgaac
    11461 tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac gaagtcgata
    11521 aaattctcgg cctcgaaatc ggtgcagatg actacatcac caaaccgttc aacccgcgtg
    11581 aactgacgat tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt actgtcagcg
    11641 aagaacgtcg tagcgttgaa agctacaagt tcaatggttg ggaactggac atcaacagcc
    11701 gttcgttgat cggccctgat ggcgagcagt acaagctgcc gcgcagcgag ttccgcgcca
    11761 tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg ctgaagaaaa
    11821 tgaccggccg tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc cgccgtattc
    11881 gtaaacattt cgaatctacg ccggatacgc cggaaatcat cgccaccatt cacggtgaag
    11941 gttatcgctt ctgcggtgat ctggaagatt aatcggcttt accaccgtca aaaaaacggc
    12001 gctttttagc gccgttttta tttttcaacc ttatttccag atacgtaatt catcgtccgt
    12061 tgtaacttct ttactggctt tcattttcgg cagtgaaaac gcataccagt ctatattacg
    12121 agtcacaaac atcatgccgg ccagcgccac caccagcaca ctggttccca acaacagcgc
    12181 gctatcggca gagttgagca gtccccacat cactccatcc agcaacaaca gcgcgaggat
    12241 aaacaacatg ctattgcgcc agcctttcaa taccgcctgc aaataaatac cgttcattaa
    12301 cgccccaatc agactggcga ttatccatgc cacggtaaaa ccgatatgtt cagaaagcgc
    12361 cagcaagagc aaataaaaca tcaccaatga aagccccacc agcaaatatt gcattgggtg
    12421 taaacgttgc gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca gtgcaatcag
    12481 cagaatggcg tacttagtcg cccggtccgt taattggtat tgatcggctg gcgtcgtcac
    12541 tgcgacgcta aacgcgggga agttttccca gccggtatca ttgcctgaag caaaacgctc
    12601 accgagatta ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg actcgctagc
    12661 ttcccgtttg gctggtagaa aatcacctaa aaaactggga tgcggccagt tgctggttaa
    12721 ggtcatttcg ctattacgcc cggcaggcac cacagaaaga tcgccggtac cgcttaaatt
    12781 cagtgccata ttcagctgca ggttctgctt acgccagtcc ccttcgggta aagggatatg
    12841 cacgccctgc ccgccttgct ctaacccggt accgggttca atggtcagtg ccgttccgtt
    12901 aacttcaggc gctttcacca caccaatacc acgcgcatcc ccgacgctaa tcacaataaa
    12961 gggcttgcct aagatgatat ttggcgcgtt gagctcgcta agacgcgaaa catcgaaatc
    13021 ggcttttagc gttaaatcac tgtgccagac ctgaccggta taaatcccta tcttgcgttc
    13081 ttccacgttc tgattgccat caaccatcaa tgactcaggt aacaaaaaat ggataaaact
    13141 tcgtttccgc tccacggttt tatcctcttc ctgcaccgta taaagctcgg tcacaggaat
    13201 agcgataagc ggcccaacga gtttttgcgg cccgctggta ctttggcgaa tcgcatcttc
    13261 cacatcgcta cggtaatcgg cacgttcgac aatcacttgc cgaatcagca ttatcggaat
    13321 caacaacagc aatactgcac caaacaaggt ggtcattttc cagaacaggg gggatttcaa
    13381 catagtcttc tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa gaatttgaag
    13441 ctatgtgaag tgacggtgaa gtcgtagcga ggccagcacg ccaccttcct gcacgttgcg
    13501 cagcgtgact tcgccgttaa acaaacgggc gacctcactg acgaacgcca accccagacc
    13561 gctgcttttt tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa tacgtgacag
    13621 cgcgtagtca ggaataccac taccggtatc cagcacctta agcgtgacgt gttcctgatc
    13681 cacttcggcg cttagcgtta tgcaaccgct ctcgggagta aaatcgatgg cgttatccag
    13741 taaattcccc agcgcctgat ccagtaacgc cggttcagca gcaacattaa cctcggtggg
    13801 cataacatgc aaagtgatgt ttttttctgc caactgcacg gtgcgcgctt cgctgacgcg
    13861 gcgaaataat gccgccacat caacagcagt cagaacgact tcctgacgat tctccagtct
    13921 tgcctggcgt agtaacgttt ccaccagcgc ctgcattcgc gcattttgtg tcagaatgtt
    13981 gtcagtaaaa cgagccacta cttcaggcgg cggaccttcg cgtaaaattt ccgccgcgcc
    14041 acgaatcgcc gccagtgggc tttttagctc atgggttaac gcataaacat actgctcaat
    14101 atagtttttc ccttccagct tcacgcgcat actttccagc gcctgcgcga gtttacgcag
    14161 ctcgctacta ccgagatcgg gaagaggaac gggcttattg tcagtgacgg aatcagcata
    14221 gcgagtgagc cgggcaatgg agcggttgat ccaccaaacc atgcctgcgc caatcaccag
    14281 tgcaatcccc aacaaaatgg cgctggccca taaaattcgc cgctcgctgc gtttaatgac
    14341 cggagccatc gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc gcgagccgcc
    14401 cataatcggc gcggcaacat acatcaccga actttcggga tcggcaggat tttgcaacgt
    14461 gctgcgcgca ccatactgac cacgcaacgt tagccagacg tcattccagc gcgaataatc
    14521 ctgtccaacg gctttatttg ccgaatcgaa caatactttg ccatgcgcat cggtcatata
    14581 gacatgatat tcattgcgca ctttgttaat gccaccaata ttggcgcgaa acgggcgatg
    14641 ttgtagctga ttaaacgcct gcgccagttg cccatgcgtt gggtccccag agagcaaatc
    14701 gggacgcgcc agctccgcaa gtaacgttgc cgtgtcgatc aacgtcccct ccgttgctct
    14761 tcgcacgccc ggtttaactt ctttgacaaa aatagccagt acgaaccagg ccgccaccgc
    14821 caccagtaaa aaatagccca gcagcaaccg catgccgata cgcattacaa gcccctcagg
    14881 ctatatccca tgccgcgatg agtattaatc ggtgaaagat cggggttgat ggcgcgcagc
    14941 ttggcacgca gcgttttaat gtggatatcg acggtgcgat cgtaggtgtc ctgcgcatct
    15001 tcccacacgc tatccattag ttgctggcgg gaccagacgc ggcctggcga cttgagtaac
    15061 gtcttcagca ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa ccagctgatc
    15121 tgcgccgcgg gttcattcaa ttcaaaatga ccaatacgga tgacgggaga cggcgtcgag
    15181 aacttcttca cccgacgcag taaggtgcgc accctggcgc acacttcgcg gggtgaaaac
    15241 ggtttagcca catagtcgtc agcgccaatt tccagcccaa gcaggcgatc gacctcttca
    15301 cttcgggccg tcaggaacag tacaggtaac gccggatgga gcgccagtaa ctggcggcac
    15361 aattcaaagc cactgatatc aggcaaacca acgtcgagaa tcatgacgtc gggaacctgc
    15421 tggcgagctt tatccagcac cggcaaacct cgctcaaaga cctcgacggc aaaaccttcc
    15481 tgctgcaaca tgtagaccag cgtgtcggct atcccttgct catcttccac taaccagacc
    15541 gtttcccgtt gcataaatcg cctctgttat tgccgccacg gcatgacagg aaccgcgcta
    15601 atcgcgtttt tcggcgaacc ttctacaact ttgtcggagt aagccagata agcgagcgcg
    15661 ttgcgtttgg cgtcataaaa gcgcacgacc tgtaacgact taaagaccag ggacgtgcgt
    15721 tttttgaata ctacctcgcc ctgagctttg ccgtttttaa tacgatccga cagttcaatc
    15781 ggcccgactt gctgacaaga aatggccgca tcggaggtat cttctgccag gcccaatccc
    15841 cctttaatac caccggtttt cgcccggctc acataacagg tgacattttt cacatcggga
    15901 tcgtcaaagg cttccacaac aattttgtga tccgggccga tcattttaaa tacggtgtcg
    15961 accgaaccaa tctcttctgc atgagccaat ggccccagca ttatcaggct taaagacagg
    16021 atcaaatgct tgtatttcat attgttacca tttttatcta ttacacgagc aattagttcg
    16081 tcacggtaaa taacatattg attaagaaca aaatctcaat tcttttattt ccgtcaagcc
    16141 ctaaaacata ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa aatgctatta
    16201 tccaattacc tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt atggatcagg
    16261 ccggcattat tcgcgacctt ttaatctggc tggaaggtca tctggatcag cccctgtcgc
    16321 tcgacaatgt agcggcgaaa gcaggttatt ccaagtggca cttacagaga atgtttaaag
    16381 atgtcactgg ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg aaatcggcgg
    16441 tcgcactacg cctgactgcg cgtccgattc tggacatcgc gctgcaatac cgcttcgact
    16501 ctcaacagac atttacccgc gcattcaaga agcagtttgc ccagactcct gcactttacc
    16561 gccgttctcc tgaatggagc gcctttggta ttcgcccgcc gctacgtctg ggtgaattca
    16621 caatgccaga gcacaaattt gtcaccctgg aagatacgcc gctgattggt gttacccaga
    16681 gctactcctg ttcgctggag caaatctctg atttccgcca tgaaatgcgt tatcagttct
    16741 ggcacgattt tctcggcaac gcgccgacca ttccgccggt gctctacggc ctgaatgaaa
    16801 cgcgtccgag tcaggataaa gacgacgaac aagaggtatt ctataccacc gcgttagccc
    16861 aggatcaggc agatggctat gtactgacgg ggcatccggt gatgctgcag ggcggcgaat
    16921 atgtgatgtt tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc ctgacggtat
    16981 acggaacgtg catgccaatg ctcaatctga cgcgccgtaa aggtcaggat attgagcgat
    17041 actacccggc agaagacgcc aaagcgggag atcgcccaat taatctgcgc tgtgaactgc
    17101 tgattcctat ccgccgttaa cgctgcagct catctaatgc aggggcgtcc agatgcgaga
    17161 tgtcccctgc ggtttcaacg acccagccag acgccagcca caggctttcc tgataatcca
    17221 cgcgcgaaat agaacagtta cgcagacgta agcggcgctc tacccatgct ggtaatccga
    17281 gaatcgtact caccagacat cccagtgcaa taccatggct caccagcagc ggtcggcttc
    17341 cctgcggtaa gtcccggcaa gattccagtg ccgcattgac gcgatcgctg agctcctgca
    17401 ttgactcgcc ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc cgacgccagt
    17461 tctcttcttc ttcggtcaga gaatcgatat gtcttttttc cagcacaccc atgtttaatt
    17521 cacgcaggcg agaatcaaag atgatgtcac agccgcaggc ctgggcgatg atttccgccg
    17581 tacgccgggt gcgtcctaaa tcgctactga taatatgcgt aatgccaagc tctttggcac
    17641 gggttgccac ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg tcagactggc
    17701 cctgaatacg tcgctcggcg ttccactgcg tttcaccgtg gcggactagg tatacctgta
    17761 acatgctttt tttccgtata ctgctgtaac gttaattttc tgagtaatgc tgattatgca
    17821 ccaagttgtc tgtgcgacca ccaatcccgc taaaattcag gccattctgc aggcatttca
    17881 cgagatcttc ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga gcggtgtacc
    17941 ggaacaaccc tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg tagcaaatgc
    18001 ccgccgttta cttccagagg ctgatttttg ggtggcgatt gaagctggca ttgatggcga
    18061 cagcactttc agttgggtgg tgattgaaaa cgccagccag cgcggcgagg cgcgttcggc
    18121 aactttgccg ttacctgcgg tgattctgga aaaagttcgt gaaggcgagg cgcttggtcc
    18181 cgtgagtcgc gttataccgg tattgatgag attggtcgta aggaaggggc gatcggcgtg
    18241 tttaccgccg ggaaactcac tcgcgccagc gtctatcacc aggcggtgat cctggcgctg
    18301 agtccgtttc acaatgccgt gtatcaggca ttgtaggacg gataaggcgt tcacgccgca
    18361 tccgacattc ggtgcacgat gcctgatgcg acgctgccgc gtcttatcag gcctacaaaa
    18421 tcaatcgctt ttcagcaaca cctcttccag ccactgacgc agctccacgg gcgcggcttt
    18481 caggctgtta gatccacgcg taatcgtcgc gatgcccgcg ccgagttcat tttttaactc
    18541 acgctgactc atttcgccac gcaacagctc ttcgacaata cgcacgcgag tccccaacgc
    18601 ttcgcgctca tctggcgtca gcatcaggtt taacaacggt aaatggagat cgttttggta
    18661 ggcattctta agcaggtcga caaaacgtaa ccactcctgg tgacgctgtt ctgccatcgc
    18721 tgctgaatag ggtgattgtt gggccataat atgtcgccat tgttagcggg ggaagcaaaa
    18781 tgactccccc ggttgtactc gctaaagagt acgatagcat atcataaacg tgcggatcag
    18841 taacgacgtc cccattccgt ggcgctcatc aacgtcggtt tatcccccat gaaatagcgg
    18901 tagtaagcat cataagccag cacgttcttc acataaccgc gcgtctcgga gaacggaata
    18961 ctctcgacaa atgccacagc gtcgatacgc ccggcgctgt tgccaagcca ggttcgcacc
    19021 cgtccaggtc cggcgttata agctgctgag gagaaaatac ggttattgcc aaactgctga
    19081 taaacatatt gcaggtaact ggtgccaatg ttgatgttcg tttccggatc cagcaattgc
    19141 ccagggctgc tataaccagg aatagagaac atcttcaccg tatgggtcgc tgtaccaggc
    19201 ataatctgca tcaggccgct ggcccctacc ggtgatttca ctttcggatt ccaggcgctc
    19261 tcctgacggg caatcgccat cgcatagctt tgcgggatct ctttcccact ggtgtaacgt
    19321 ttgaaaagat cgttgtaagc cagcgggaat cgctcttcca gatgatccca aagcttcccg
    19381 gcgatcgttg cctgaacgct aagatcccac cattggttat tgaaagcata ccgagccagt
    19441 tgagcctgct ctgtttttga cttgctcttc accagattgg cccactcgct acgcgcggta
    19501 ttatcgagat tccagtacat caactcgcgc acgcgcgcca tctccggccc ctgagtcagg
    19561 gtgctgtcaa cattctgcgg cgctttatca atcttcagct catactcttc accgatgcgt
    19621 tgtgctgcaa ccatcgggta gaaaccacgc tgttgtatga gttgatgcaa aatctctttc
    19681 gcttcagctt cacgtccgcg ttccagcaat aaatccgcct gccagtaacg ccattcatct
    19741 ttctcttttg cttccatcgg cagacgcgcc agccaggtat tcaggccgcg acgatcgccg
    19801 gtgccaagcg ccattcgcac acggcgttca atcaacgacg tggattgcga gcgcataatg
    19861 gcgtcatcgc gccatttcgc ctgctcatcg gtgacatcat tgcccatcaa acgccaggcg
    19921 acgatatcgc gcagctcctg aatctgatct tcattaagct gctgcgcctg ggcaagcgac
    19981 gggatcatca gccgtgcatt ttcggcatcc tgccgcgcca cactggcaaa cgccaccgcc
    20041 gccatttgac gggtaaaatc ggtcgcgccg gttgtacgcg cgaaggtcag taccgtatta
    20101 gggttgttcg ccagtgaaat gattgccgag gcgatagtct ggtaatcggc aggcatctgc
    20161 cctgccagca ctgttaccag gcctgtgtta cccgctttca tcgccagacg gatacgctct
    20221 aaatacgcca gcggatcttg tttacctgac gcacgccaga cgctaaataa cttgtcacag
    20281 gcgttaggct ggctcttgcc ggttagccac agctctttcg ccccttgcca ggcttcttca
    20341 ctctgcccgg tgttccattt cgcatagtag taattacatt gcgcttcggt agttccgggc
    20401 ttttccgggc taaaggctaa caagccacgc cagtcttcac gccgcgccag ttcattgacg
    20461 aaacgagatt gcagcgtgcg agcgggagga agcgtggggt tagcgcgaac aaagttagtg
    20521 accgtcaccg ccggttgatt catcaggtca tcggtgatct ggcggtattc caggtaggga
    20581 taaagcggat aatccttcag tccaggcatc atttgttcga ccacatccat ctgtcgatta
    20641 tcccaggcct gcttgatttg cgcgtaacgg ctacgctgct catccagtga gtcggctcgc
    20701 gccacgctgc tgaccgtcag cagacagaca ccggcagcca acagccgcca ggtaacttgt
    20761 ttggcttttt ccacaagcgc atcctctaag tgtaaataca tcaatgcagc gtcatgccgc
    20821 gtaatagatt tatgctaacc agtcattgcc gtttacgcca cgttacggac acttttttac
    20881 ttttactgcg aaggtgatcg accaactggg attaggcggg gaaacaggct acacttcgcc
    20941 tttgaaaact catcatcacg ataaacatag aggcgaagtc caacgtggct caattcgttt
    21001 ataccatgca tcgtgtcggc aaagttgttc cgccgaaacg tcatattttg aaaaacatct
    21061 ctctgagttt cttccctggg gcaaaaattg gtgtcctggg tctgaacggc gcgggtaagt
    21121 ccaccctgct gcgcattatg gcgggcattg ataaagacat cgaaggtgaa gcgcgtccgc
    21181 agccagacat caagattggt tatctgccgc aggaaccgca gctgaacccg gaacacaccg
    21241 tgcgtgagtc cattgaagaa gcggtttcag aagtggttaa cgccctgaaa cgcctggatg
    21301 aagtgtatgc gctgtacgcc gatccggatg ccgattttga caagctggcc gctgaacaag
    21361 gccgtctgga agagatcatt caggctcacg acggtcataa cctgaacgta cagctggagc
    21421 gtgcggcgga tgcgctgcgt ctgccagatt gggacgcgaa aatcgctaac ctctccggtg
    21481 gtgagcgtcg tcgcgtggcg ttgtgccgcc tgctgctgga aaaaccagac atgctgctgc
    21541 tcgacgaacc gaccaaccac ctggatgccg aatccgtggc gtggctggaa cgcttcctgc
    21601 acgacttcga aggcaccgtg gtggcgatta ctcacgaccg ttacttcctc gataacgttg
    21661 caggctggat cctcgaactg gaccgcggtg aaggtattcc gtgggaaggt aactactcct
    21721 cctggctgga gcagaaagat cagcgtctgg cgcaggaagc ttcacaagaa gcggcgcgtc
    21781 gtaagtcgat cgagaaagag ctggagtggg tacgtcaggg aactaaaggc cgccagtcga
    21841 aaggtaaagc acgtctggca cgctttgaag agctgaacag caccgaatac cagaaacgta
    21901 acgaaaccaa cgaactgttt attccacctg gaccgcgtct gggcgataaa gtgctggaag
    21961 tcagcaacct gcgtaaatcc tacggcgatc gcctgctgat tgatgacctg agcttctcga
    22021 tcccgaaagg ggcgatcgtc gggatcatcg gtccgaacgg cgcgggtaaa tcgaccctgt
    22081 tccgtatgat ctccggtcag gaacagccgg acagcggcac catcactttg ggtgaaacgg
    22141 tgaaactggc atcggttgat cagttccgtg actcaatgga taacagcaaa accgtttggg
    22201 aagaagtttc cggcgggctg gatattatga agatcggcaa caccgagatg ccaagccgcg
    22261 cctacgttgg ccgctttaac tttaaagggg ttgatcaggg taaacgtgtt ggcgaacttt
    22321 ccggtggtga gcgcggtcgt ctgcatctgg cgaagctgct gcaggttggc ggcaacatgc
    22381 tgctgctcga cgaaccaacc aacgacctgg atatcgaaac cctgcgcgcg ctggaaaacg
    22441 ccctgctgga gttcccgggc tgtgcgatgg ttatctcgca cgaccgttgg ttcctcgacc
    22501 gtatcgccac gcacatcctg gactaccagg atgaaggtaa agttgagttc ttcgaaggta
    22561 actttactga gtacgaagag tacaaaaaac gcacgctggg cgcagacgcg ctggagccga
    22621 agcgtatcaa gtacaagcgt attgcgaagt aatgcgtaaa atgccggatg cggcgcgaac
    22681 gccttatccg gcctacaaaa ccagcaattt caatacgttg taaaactgta ggcctgataa
    22741 gacgcggtaa gcgtcgcatc aggcattgag caccgaatgc cggatgcggc gtgaacacct
    22801 tttccggtct acaacagcct tagcatcctc aatttcgtgg cgggtcaccc gtgcaggttt
    22861 tttcaaacct tgtttaatct cgacagcctc ttccagagag gttttcaact catcaaaaaa
    22921 actcatttta tacctcgcct ttgagcagtt tcgtcacggt tatccttgct cccccatcat
    22981 ctcccgcacc agctccacgc agcgcaggaa acggctgtcg taatcgtcct cttcaacccg
    23041 cacgaattcg atattgttct cttccagcat ctccaccagc aagttctgga actctttgcg
    23101 atccaccgaa ctgccgaggc tgcgtaaacc atccgccacc cacggcgtgt tgttctccag
    23161 caggatcacc agatcgaaac ggtattcatc aatcagcgcc tgcacgaacg gatgctcacg
    23221 cccttcgtac tttttgcaga acgcctgggt agtgacaaaa tcggtgtcaa taaacgccac
    23281 tttattggcg tatttcactg caaaatcaat gtattgtgcg tggcccaacg cgattttatc
    23341 gtagtcggaa tactgcaatg cgatctcatc accgccgagg tgtgaaaaga cataatcgcg
    23401 gccatattcc cacgcactgg tggtgttgaa gatattggca agtttgttta ccagggtgga
    23461 tttaccgctc gactcgccgc caaggatcgc cacggtacgc acaaaaaacg gcttcacttc
    23521 ggtaggaata tattcccagt agcggaacgg gttttcgcgg atctgcgcac cgctgatact
    23581 cataaaggta cgtttcggat cgaccagcac cgtctcgatc cccagatgtt ccatatactg
    23641 tggcgcatcg gcttcttccg aggtgtagat caggtccggc tggatcccct tttcagccat
    23701 aaactttttg atgccgttgc tccacacatc ccagccatgc ggatacggct ccatgccctc
    23761 ttcgttgaaa gcatgaatac ggatattttt ctgatattta aaggtttgca atagccagcg
    23821 cagacgatcc ggcaccgtgg gctgctgcga catggcgctg tcttcaaaca acgcgcggtc
    23881 gcgggtatcg tcaaaaccca taatgatatg cagctcgtca acctggctac aggcgcgctg
    23941 gataaggtag atatgtccgg tatgcagcgg gtagaactta ccgaatacga caccaatggt
    24001 tttcttctgc cggggaaatt caagccccaa aaaacggtgc aatgcctcca gcttttgcgc
    24061 gctggggctt ttaattttgg cattcagtaa ctggcttaaa taccctttgg tcataccgct
    24121 ggcatcagct acctgctgta gcgtgcagcc ctgttgcttg atggcagttt tcaggtaatc
    24181 aaatgacgac atatctccct ccgtatctct cattataagt cgtcgaacac gctaagcgcg
    24241 tcggagagtt ttttaacgcc aaaaatctgc atcccttccg gcgctttttt cggtacgtta
    24301 gccgccggaa caatcgcccg gcgaaaaccg tgtttcgccg cttcagaaat ccgctcctgg
    24361 ccactgggca ccgggcggat ctcccctgcc agcccgactt caccaaacac caccagatcc
    24421 tgtggcagcg gtctgtcacg caggctggaa accatcgcca gcagtaacgc taagtcgccg
    24481 ctggtttcgg ttaccttcac gccgccgacc acgttcacaa acacatcctg atcggccatt
    24541 tgcagaccac cgtgacggtg caagacagcc agcaggattg ccagacggtt ttgttccagc
    24601 cccactgcca cgcggcgcgg attcgccatt atcgagtgat cgaccagcgc ctgaatctcc
    24661 accagcaacg gacgcgttcc ttcccacacc accatcacgg agctgccgga ggtcacttca
    24721 tctccgcgac ttaagaaaat tgccgaaggg ttgctgactt cacgcagccc ctgttcggtc
    24781 atcgcgaaga cgcccagctc attcaccgcg ccgaagcggt ttttatggct gcgtaaggtg
    24841 cgaaaacggg agtcggcatc gccatccaga agcaccgaac agtcgataca gtgttccagc
    24901 actttcgggc cagccagcga accatctttg gttacgtgtc caaccatgac aatcgccaca
    24961 ccgcgcgttt tggcgaaacg cgtcagataa gccgccgttt cacgcacctg cgccacgcta
    25021 ccaggcgatg attgtacatc cgccatatgc atcacctgga tcgagtcaat taccatcagc
    25081 ttcggttgct cttcttcggc aatcaggcag atctgctcga tgctggtttc cgacaacata
    25141 ttgagattgt cagtcggcag gccaaggcga tgagcgcgca ttgccacctg ttgcagcgac
    25201 tcttcgccgg tgacatacag cgttttcatc tgctgggcca gtttgcacag cgtttgcagc
    25261 aacagcgtgg atttccccgc accagggtta ccgccaatca gaatggcact tcctggcacc
    25321 acgccgcctc ctagtacgcg gtcgaactct ttaaatccgg tggaaaaacg cggcagctct
    25381 tcaaggctga tatcggagag tttctggact tttgccaccc cggcgctacc ggcatagccg
    25441 ctgagacgct cgttacgcgc caccgttggc gacgcagcaa gacgcacctc ggtgatggtg
    25501 ttccaggcat gacaggcact gcactgcccc tgccagcgcg gataatcggc cccgcattca
    25561 ttacaaacaa aggcgcgttt tggagctttt gccacggtgt acctcgttaa tgctgtgccg
    25621 cccgcaggat gacgggcagg caattacttc tgattcaggc tgccagagag gatgcagaat
    25681 acccccatca ggtcagcgtg acggatggtg acttccgcct tttcattcac ttttggcttg
    25741 gcatggtagg caatccccag ccctgctgct ttgatcatcg gcaggtcatt agctccatcg
    25801 ccaatcgcca cggtctgcgc cagcgggatt tcatactcct gcgcgaggcg agtcagagtt
    25861 ttcgctttgt actgcgcgtc tacgatgtcg ccgatcacat tgccggtaaa tttaccgtcc
    25921 atgatctcca gttcattggc taccacggct gtcaggcgca gcttgtcgcg caggtattca
    25981 gcaaagaaag taaagccgcc ggaggcaatc gccactttcc agcccagcgt ttccagcttg
    26041 agcaccagct gcgttaagcc tggcatcagc ggcagatttt cacgcacctg ttgcagaata
    26101 ttggcgtcag cgcctttcag cgtcgccacg cggctgcgca ggctggcggt aaaatcgagt
    26161 tcgccgcgca tcgcccgttc ggttacctcc gccaccatct cgcccgttcc ggccagcttg
    26221 gcaatttcat caatacattc aatctggatg gcggtggaat ccatgtccat caccagcaaa
    26281 cccggcgtgc gcaggtgcgg gattttcccc agcggggcga catccagctg cgcttcgtgg
    26341 gccaggcgtg tagcccgtgc ggtgagtgaa cctgccagac gaatcacctg ataatcttcc
    26401 acgcaccagg cggcaacaat caccatcgcc gcacccagtt tgctctggta ttgggtcaga
    26461 cgctgtttat ccagcccacg accatacagc agccagccgc tacgacctgc gtggtaatcc
    26521 agtggcatca cttcatcacc acttaatgaa agaggcagac ccggccataa agagacatct
    26581 tcaggcaggt cgcaccaggt aatgttaggc attaaggctc ctgtaaaatc gttcgaagca
    26641 gggaaaataa cgcatgaggc taccttgtat ccattgcttc tggcaacatt aagtctcaaa
    26701 ttttcaaagg gtggaagatg gctcgcacaa aactgaaatt ccggctacat cgggcagtga
    26761 ttgtcctgtt ctgtcttgcc ttgttagtgg cactgatgca gggggcgtca tggtttagtc
    26821 aaaaccacca gcgacagcgt aatccacagc tggaagaact ggcccgcacc ctggcgcgtc
    26881 aggtgacgct gaacgttgca ccgctgatgc gtaccgactc accggatgaa aaacgcattc
    26941 aggcgatcct cgatcagtta acagatgaaa gccgtatcct cgacgcgggt gtgtatgacg
    27001 aacaaggcga tcttatcgca cgttctggcg aaagcgtcga agtgcgcgat cggctggcgc
    27061 tcgacggtaa aaaagcaggc ggctatttta accagcagat tgtcgagcca attgcgggta
    27121 aaaacggacc gctcggctat ctgcgcctga cactcgacac ccatacgctc gccaccgaag
    27181 cccaacaggt ggataacacc actaacattt tacgcctgat gttgctgctc tcactggcaa
    27241 tcggcgtagt gctgacccgc acgctgctac agggtaaacg cacccgctgg cagcaatcgc
    27301 ccttcctgtt aaccgccagc aaaccggtgc cggaagagga agaaagcgag aaaaaagagt
    27361 gacccattac tacaagaaag gaaatcgtta tgtccacatt acgcctgctc atctctgact
    27421 cttacgaccc gtggtttaac ctggcggtgg aagagtgtat ttttcgccaa atgcccgcca
    27481 cgcagcgcgt tctgtttctc tggcgcaatg ccgacacggt agtaattggt cgcgcgcaga
    27541 acccgtggaa agagtgtaat acccggcgga tggaagaaga taacgtccgc ctggcgcggc
    27601 gcagtggcgg tggcggcgcg gtgttccacg atctcggcaa tacctgcttt acctttatgg
    27661 ctggcaagcc ggagtacgat aaaactctct ccacgtcgat tgtgctcaat gcgctgaacg
    27721 cgctcggcgt cagcgccgaa gcgtccggac gtaacgatct ggtggtgaaa accgtcgaag
    27781 gcgaccgcaa agtctcaggc tcggcctatc gcgaaaccaa agatcgcggc ttccaccacg
    27841 gcaccttgct actcaatgcc gacctcagcc gcctggcaaa ctatctcaat ccggataaaa
    27901 agaaactggc ggcgaaaggc attacttcag tgcgttcccg cgtgaccaac ctcaccgagc
    27961 tgctgccggg gctcaccatg agcaggtttg cgaggccata accgaggcct ttttcgccca
    28021 ttatggcgag cacgtggaag cggaaatcat ctccccggac aaaacgccag acttgccaaa
    28081 cttcgccgaa acctttgccc gtcagagtag ctgagagtgg aacttcggtc aggctccggc
    28141 attctcgcat ctgctggatg aacgctttag ctggggcggc gtggaactgc atttcgacgt
    28201 tgaaaaaggc catatcaccc gcgcccaggt atttaccgac agcctcaacc tcgcgccgct
    28261 ggaagccctc gccgggcgac tgcaaggctg cctgtaccgc gcggatatgc tgcaacaaga
    28321 gtgcgaagcg ctgttggttg acttcccgga ccaggaaaaa gagctacggg agttgtcgac
    28381 gtggatagcg ggggcggtaa ggtaactcgc tgattaaaat atccaaggaa ggaaatatga
    28441 tcgatctgga aaaccaggaa cgggagatta tcaatttaat gttcagccag cgtatttcct
    28501 ggctagccgc cgtgcgtatc aggcataaat tatcgcttgc tgaagtaagt aaaatgttag
    28561 gtatctcgat taattctcta aaacagatcg aaaagacgga acggcttagc tcaaacatca
    28621 aaagcaaaat ggcagaaatt tatggttgtc caccagagtt gctgatatgc ccttcctgga
    28681 tgacggctga acataaatga atagattcgt gaatagatac ttgaatgttt tttattttct
    28741 atccatatga aaaataatag taaatctata aatcagttgc tattgatgaa tagatactaa
    28801 aaagccggag cagtccccca ctccggctta acacgacaat cgcctttcag cgaaacacaa
    28861 ttactcttta tcgcccagca gaacggattc cagtgcgatt ttgatcatgt cgttgaaggt
    28921 ggtctgacgc tcagcggcag tggtctgctc gtgagtgcgg atgtggtcag acacggtgca
    28981 gatggtcagg gctttcgcgc caaactctgc agccacgccg tagataccag ccgcttccat
    29041 ttccacgccg aggatgccgt atttttccat cacgtcgaac atttcgccgt ccggagagta
    29101 gaacaggtca gcggagaaca ggttacccac gcgagcatca acgcccagtg ctttagctgc
    29161 atctactgcg ttacgcacca tgtcaaagtc agcgatagcg gcaaagtcat ggtctttaaa
    29221 acggatgcgg ttaactttgg aatcggtgca ggcccccata ccgataacga cgtcgcgcag
    29281 ttttacgtgc ggcagaactg cgccacagga accaacgcgg ataattttct tcacgccgaa
    29341 atcggtgatc agttctttgg tgtagatgga gcaggacggg atacccatac cgtgacccat
    29401 tacggaaatt ttgcggcctt tgtaagtacc agtgaagccc agcataccgc gaacgttgtt
    29461 cacttcacgg gcatcttcaa ggaaagtttc agcaatatac ttcgcacgca gcgggtcgcc
    29521 tggcatcaaa actacgtcag cgaaatcgcc catttctgca ttaatgtgtg gggtagccat
    29581 tgttttatcc tttttgtgac ataacaaagg cggagtcaac gctccgccca aatccatcag
    29641 aacatggctt tgccatattc catatcagaa gtaccaaaat attttgccag agtctggccg
    29701 atatccgcga aggtttcacg gtgacccagt gagcccggtt ttactttcgg gccatatacc
    29761 agtaccggaa tgtgttcacg cgtgtggtca gtaccggtcc aggtcggatc gcaaccgtgg
    29821 tcagcggtga ggatcaggat gtcgtcatcg cgcagcagag acatcagctc cggcagacgg
    29881 cggtcgaaca gttccagacc cgcagcataa ccggcgacgt cgcggcggtg gccccaggaa
    29941 gagtcgaagt caacgaagtt ggtgaagacg atggtgttat cacccgcttc tttcatctct
    30001 ttgatggtgg cgtcaaacag cgcgtccaag ccagtcgctt tcactttttt ggtgataccg
    30061 cagttggcgt agatgtctgc aattttaccg acagaaacca cctggccgtg tttttcatca
    30121 accagtttct gcagcacggt cggtgctggc ggctcaacag ccaagtcgtg acggttaccg
    30181 gtacgctgga agttaccagc tttgtcaccg ataaacggac gagcgataac acgaccgata
    30241 ttgtagccgc cgttggtcag ctcttcacgg gcgatttcgc acagttcgta gagtttatcc
    30301 agaccgaaag tttcttcatg gcaggcaatc tggaacacgg agtcagcgga ggtatagaaa
    30361 atcggcttgc cggttttcat gtgctcttcg cccagttgat ccagaatgac cgtaccggaa
    30421 gagtggcagt taccgaggta acccggcaga ttagcacgtt cgaccagttt atccagcagc
    30481 tcttgcggga agctgttttc gtgatcggag aaatatcccc actcaaacag aaccgggaca
    30541 ccggcgattt cccagtgacc agacggggta tctttaccgg atgacatttc gtgcgcccat
    30601 gcgtacgcgc cgataacttc agcgttgccg tccattcccg ccggaatgaa accggtagaa
    30661 ccttcgtgtg ctttcgccag ccccagacgg gtcagatttg gcagattgag cggcccttta
    30721 cgaccattat cagcttcgcc tttggcacaa gcttctgcga tatgacccag ggtgtcagcc
    30781 ccgacgtcac caaagcgttc tgcatcttct gtagcgccga tgccgaatga gtccagcacc
    30841 ataataaatg cacgtttcat atgttctccg tactcagtgc ttcaaattta aatgatcaga
    30901 tcagtatacc gttattcgct gatacggcga tagacagttg gtgtgctttc cggtgcgtta
    30961 tcggcaagtt taattgccgc tttcaccgct ttcgccgctt cctgccagct gttttcgtct
    31021 ttcgcgagga taaccgccag cggacgctga ccgtctacct ggtcgcccag acgcgccata
    31081 tcagtaaagc cgacgctgta atcgatggtg tcagatgcct gacgacgacc gccgcccatt
    31141 gcaaccactg ccatccccag cgcgcgggta tccatttcac tgacaaaacc ttcggtatca
    31201 gcatagactg ctttcgtcag catcgctgtc ggcagatact tcgcgtagtt ctcaacgaag
    31261 tcggtcgggc ctttttgtgc cgctaccata cgaccaaaga cttctgccgc tttaccgttg
    31321 tccagcaccg cctgcaattt cgcgcgcgct tcggcgtcat ctttcgccag tttgccggag
    31381 atcagcatct ccacgcacag cgccatcgtg acatcaaaca gacgcgggtt acgatactca
    31441 cccgtcagga actgcaccgc ttcacgaact tcaaccgcgt tacctgcact ggaggccagt
    31501 acctgattca tgtcggtgag cagcgcggtg gtgcgcacgc cagcgccgtt agccacgcca
    31561 acaatcgctt cggcaagggc ttcagagagt tcgtaggtcg gcataaacgc gccgctaccc
    31621 actttcacgt ccatcaccag cgcgtccaga ccttccgcca gtttcttcgc caggatcgag
    31681 gcagtgatca gcgggatgga gtccacggtt gcggtaatat cacgggtcgc gtagaaacgt
    31741 ttatcagccg gagccagtga actggtctga ccgataatcg ccacgccgac gtctttaata
    31801 atttcgcgga aacggttgtc atccgggaaa atgtcgaagc cagggatgga ttccagtttg
    31861 tcgagcgtac cgccagtatg accgaggccg cgaccggaga tcatcggaat atagccgccg
    31921 caggctgcga ccatcggccc caacatcagc gaagtgacat cgccgacgcc gccggtcgag
    31981 tgtttatcaa caatcgggcc attcagatgc aggcttttcc agtcgagaac ggttcctgaa
    32041 tctcgcatcg ccatggtcag cgagacacgc tcaggcattg tcatatcgtg gaagaaaatg
    32101 gtcatcgcga gggcggcaat ctgcccttcg gaaatagtgt tgtcgcgaat accgttgata
    32161 aagaaacgaa tttcttcatc gctcagcgca tgaccatcac gttttttacg aataatttct
    32221 tgtgcgagaa acaaggtaac ctcccgagga aaagagtatg aggcggcttt tgccggatgc
    32281 gtctacgcct tatccggcct acgggtagat acgtctcgta ggcctgataa gcgcagcgca
    32341 tcaggcgtaa agcatcttac ttagtagctg ctggcgctct taccgtcgcc gtggcccagc
    32401 gctttcagca ggcttgccag caggctggaa gcaccaaagc ggtagtgacg cgcatcagcc
    32461 cagtcagcac cgaacagttc atctgcgatg gcgagatatt tctgcgcatc ttccgcagta
    32521 cgcacgccgc ccgccggttt gaaaccaacg gttttttcta cgcccatatc acggatcact
    32581 tccatcatga tgcgagcgct ttccggcgtc gcgttcacag ccactttacc ggtagaggtt
    32641 ttgatgaagt ccgcacccgc tttgatggag atttcagacg ctttacggat cagcgcttcg
    32701 tctttcagtt cgccggtttc gatgatcact ttcagcagta cattcgctgc cgcgcaagcc
    32761 tctttacagg ctttcaccag gtcaaaacca acctgctcgt cacccgccat cagcgcgcgg
    32821 tacgggaaca ccacgtcaac ttcatcagca ccgtaggcga ttgccgcacg ggtttctgcc
    32881 agcgcgattt cgatgtcgtc gttaccgtgt gggaagttgg ttaccgtagc gatacggatt
    32941 tccggggtgc cctgctcttt cagagttttg cgagcaatcg ggataaaacg aggatagata
    33001 cagatagcgg cggtattgcc gaccggagtt ttggcctgat gacacagagc aattactttc
    33061 tcgtcggtgt cgtcgtcatt cagggtggtc aggtccatca atttcagtgc acgcaggctg
    33121 cttgctttca gatcagtcat ttcattctcc tggcttgtcg ccaataaaat tcaccttgcg
    33181 agtttgttag tattctaaca tctactccgc aacacacttc gatacacatc acaattaagg
    33241 aaatctactt acaagtttgc atcactgtaa tgcgatctgg ttcaaataat tcactttcaa
    33301 atgaatgcgt cagtggtggc aaacgcatca ggatcaaagt gaacatcacg aaacttctta
    33361 caatggcgca tcaccttcgg cataaaagga aaagaagatg cccacttctc atgaaaatgc
    33421 actgcaacaa cgttgccagc aaattgtcac cagcccagtg cttagcccgg agcagaagcg
    33481 ccattttctg gcactggaag cagaaaacaa tctgccttac ccacagcttc ctgccgaagc
    33541 ccgccgcgcg ctggatgaag gtgtaatctg cgatatgttt gaaggtcatg cgccgtacaa
    33601 accgcgctat gtcttacccg attacgctcg ttttctggcg aacggttccg aatggctgga
    33661 gctggaaggc gcgaaagatc ttgatgacgc actctctctg ctgaccattc tttaccacca
    33721 cgtaccgtcg gtcacatcga tgccggtcta cctggggcaa ctggatgcgt tgttgcaacc
    33781 gtatgttaga attctaacac aaaacgagat cgatgttcga ataaaacgtt tctggcgtta
    33841 cctcgacaga accctgccag atgcctttat gcacgccaat atcggcccgt ctgattcgcc
    33901 cattacccgc gcgatcttac gtgcagacgc gcaggtttca ccgaacctga cctttatcta
    33961 cgatcctgaa atcacccctg atgacctgct gctggaagtg gcgaagaaca tctgtgaatg
    34021 tagcaaaccg cacatcgcca acggtccggt gcatgataaa attttcacaa aagggggcta
    34081 cgggattgtg agctgttaca actcactgcc gctggcgggt ggtggcagca cgctggtacg
    34141 ccttaacctg aaagccattg ccgagcgcag cgaatcgctg gatgacttct ttacgcgcac
    34201 tctaccgcac tactgccagc agcagatcgc catcatcgat gcgcggtgtg aattcctcta
    34261 tcaacaatca cacttctttg agaatagctt cctggtgaaa gaagggctga ttaaccctga
    34321 acgttttgtg ccaatgtttg gcatgtatgg gctggcggaa gcggttaact tgctgtgtga
    34381 gaaagaaggg attgccgcgc gctacggtaa agaagccgcc gcaaatgaag tgggttatcg
    34441 catcagcgcg caactggcgg agtttgtcgc caataccccc gtgaaatatg gctggcaaaa
    34501 acgcgccatg ttacacgcac agtcggggat cagttccgat atcggcacca cgccgggcgc
    34561 gcgtttgccg tatggcgatg agccagatcc gatcacccat ctgcaaactg tcgccccgca
    34621 tcatgcttat tattattccg gcatcagcga cattctgacg ctcgacgaaa ccatcaaacg
    34681 taacccgcag gcactggtac agctttgcct cggtgccttt aaagccggaa tgcgtgaatt
    34741 taccgccaat gtcagcggta acgatctggt tcgcgttacc ggttatatgg tgcgtttgtc
    34801 ggatttagaa aaatatcgcg ccgaaggttc acgcaccaac accacctggc tgggcgaaga
    34861 agccgcacgc aacactcgta ttctggaacg ccagccgcgc gtgataagcc atgaacagca
    34921 gatgcgcttt agtcagtaag attatcccct tctcctgcgt tgacgggcca ggcagtcgtc
    34981 tggctctgtt tttgcagggc tgcaatctgc gctgcaaaaa ctgtcacaat ccgtggacga
    35041 tgggacgttg caatgactgt ggggagtgcg tgccacagtg tccgcatcag gcgttgcaga
    35101 ttgttgacgg caaagtggtg tggaacgctg tggtctgcga gcaatgtgat acctgcctga
    35161 agatgtgtcc gcaacatgcc acaccaatgg cgcaatccat gagcgtggac gaagtgctta
    35221 gccatgtgcg caaagcggtg ctgtttattg aagggataac ggtaagcggc ggcgaagcca
    35281 cgacccaact gccgtttgta gtggcgctgt ttactgctat caaaaacgat ccgcaactgc
    35341 gccatctcac ctgtctggtg gacagtaacg gtatgttgag cgaaaccggc tgggaaaaat
    35401 tactcccggt gtgtgacggc gcaatgctcg atctcaaagc atggggtagc gaatgtcatc
    35461 aacaattaac cggacgcgat aatcaacaga ttaagcgcag catctatttg ctggcagagc
    35521 gcggcaagct ggcggaactg cgtttgctga tgatcccagg ccaggtggat tatttgcaac
    35581 acatcgaaga actggcggcg tttatcaagg gacttggtga tgttccggtt cgcctgaacg
    35641 cgtttcatgc ccacggcgtg tatggcgagg cgcaaagctg ggcgagcgcc acgccggaag
    35701 acgttgagcc gttggctgat gcattaaagg tgcgcggggt gagccggttg atatttccgg
    35761 cgctctattt gtgacatgcc ggatacggcc tacggttcgg cacagacttg taggcctgat
    35821 aagacgcgtc aagcgtcgca tcaggcattg tgcgccaact gccggatgcg gcgtgaacgc
    35881 cttatccggc ctacggttcg gcacaaacct gtaggcatga taagacgcgt taagcgtcgc
    35941 atcaggcatt atgcgccgac tgccggatgc tgcctacgaa gggcaaacta cccacgaacg
    36001 ttaaacaccg catacgtgtt attaagcaac acttcggcaa tctcatccgc gggttccggg
    36061 cgtaactcgc aaagcacggc aaacacacgg gcagcctgtt ccgggcggtt aggctgcccc
    36121 tgaaaaccgt tgagcggcat atccggcgca tcggtttcca gcaacaacga tgccagcggt
    36181 aattttgcga tgacatcgcg ggttttactg gcgcgtggat aggtgatagt accgcctacg
    36241 ccgattttgt agcccagctg tacaaaccgt tcagcctgtt gctggctgcc agaaaatccg
    36301 tggacaacac cagcacgcgg caaatcgtgg tgtttaagat gcatcgccag tttgtcgtgc
    36361 gtgcgccgtg aatgcaggat caccggcaga tcgtagcgtt tcgccagttt cagttgttcg
    36421 tcgagtaacc actgctgcct ctcaaattgc ggatcgtcgc cgaagagatc cagaccgatc
    36481 tcccccaccg ccaccacctt cgccggacgc ctttccagcg cctgctgtag ctgctcaaga
    36541 gacacatcgc tatgtttttc caacatacca ggatgcaagc ccaatgcggc atacagcggt
    36601 tgataatttt ccgctaacgc ctgcacgcgg gcaaaatttt ccgcctctgt cgccggaaca
    36661 atgatcttgc ctacgcccgc ttgtgccgcg cgttgcaggc tggcctcttc atcgccaata
    36721 aaaggcggga aatcaaaatg gcagtgagtg tcgataaaac ggcaaatcaa gccagatcct
    36781 cattgttaaa tgtcgggtcg ttcgcctgcg gagcgtcgct cacttcggca accagcgtat
    36841 cgttggcgac tggcgcaggc ggaatgacaa tcgattccgg cgtcaccact ggcaccagat
    36901 ggcgggtaag cggcgctttt tcagttaata gcttgccaac cgtggcgagg aaataacgac
    36961 cgcataaacg cccaagttta tagtcttcac gcagcgccgg aatccgactg ccaagcgcga
    37021 tactatgtaa tggcttcggc ggataaattt cggatatccg cagcttgccc ggtggtttct
    37081 caataaattg ctgaatgtca cgatagctgg tttcatgatg ctgcaccaga ttgaccagcg
    37141 gctgcaggct actgtcaccc agccagcgtt ccatgcgttt gaaccactgc ggcgtgtagt
    37201 acatttgtga cggcacagtg cggatgacga ccaacgtttt agcgccctgc cttgccgcct
    37261 ctttaaccgg aatcgcatca ctgatcccgc catccaggta gttaatgcct tccagcgaca
    37321 ctccgctacg ataaaagcca ggtatcgcac tggaggcgcg aatcacatcc agccagtttt
    37381 gtttggttgg taaaaagtaa ttcggctcgt agtcatcctg acgacaggcg cacatataaa
    37441 acgatttgcc gctgtcaaac aaccgcgcgg cggtatccat ttgcaacggc atctggcttg
    37501 cggtggcctc caccagccag tcgagatcga taagatttcc tccacggaca aagcgcaatg
    37561 gatcgaaaaa ttcgcgtttt gtggtatagc gcatgatgac tttgcgcgcg taaccgggct
    37621 gattgcagat aaacgccgag aggttctgcg ccccggcaga tgtgccgaga taaagatcga
    37681 aaggattaaa ctgcgcgcgc ataaactcat ccagcacgcc agccgtgaaa attccacgct
    37741 gtccgccgcc ttcgcacacc agagctattc gtccaggctg gaacggcctt agcgacaacg
    37801 gcgcaatatt accaagcgtt acaggtattc gctgccccac ttctgccttc ctgtttttgt
    37861 tgtgaaagaa agacaaagta acgcaattta ttattcgcta aaaccgcaaa agccagtccg
    37921 ctggactggc tttggataaa attttcttaa tggtctgaaa tctagggtcg ttttcggccc
    37981 atgaacaaac tcactaggaa cagaataatc ccgacgacaa agacaatttt agctgcgcct
    38041 gcagcggtac cggccagacc accaaaccca agtgcggcgg cgattaacgc gataaccaga
    38101 aatatgatgc cccaacgaaa cataagcgtc tcctttacca tagttaatgt caccgcttga
    38161 tatgagcgtg aaaatcactc aatgcgatat ttttagtgtg gtgcacatcg cgcctcccga
    38221 caaagttcgg gaggacgaat tacttagttt tcagatcatt tttaacgctt ttcacaccat
    38281 ctaccgcttt ggcgatactt tcagcacggt cactttgtgc ctgagaatct acggtaccgg
    38341 agagctgaac cacgccatcg gtggtttcaa ctttcacttt acgtgaaggg acgatatcgt
    38401 ccgccagcag tttggctttg atttcactgg tggtggcggt gtcacccgcg tagcccttca
    38461 ccgagccttc tttagcgtcg cgaacgtgca gtttgtcgct gacagaggtc acgccttcaa
    38521 cgcctttcgc cactttcact gcctcttcgg cctgggcctg actttcaacg aaaccgctca
    38581 gggtcacgac tttttgatcg gttttgacag agatatcggt gctcttgatg ttgtcatgat
    38641 ccaccagcgc cgccttcact ttcgcggtga tggcgctgtc atccatgaaa ttaccgactt
    38701 tattcataga gctatcgact ttttgccctg cgctttcatt ggtagtctgc gcgttgtttt
    38761 ccgcgtaggc agagccggtc gcgacggcag aggtcaacat tacagccagc agagttttcg
    38821 aaatcttcag tcttgtcata gtcatcgatt tattcctgta tgtttgctcg taatttgagc
    38881 ctagcaacac gaggttgcat tgctgaatag ggagagactt caccctctac agaagtcaat
    38941 ggtcgccatc acaaaagcga tgggtgatga ataacgacca ttacagcctc tgaatcagtt
    39001 attaatatcg gtagaatgac aatcgacggc tttagatact gatatctacg cattgaacgg
    39061 tatttaacgc catcagaaat gtcatcactt tgttaaatat agatcacaat tttgaaaccg
    39121 ctcgggatat cagcgaaaac ataagcaaaa gtgaatgttt taagaacatt ccgtaagcgg
    39181 ctaataagga agggaaattg acagggcgca gcggctaccg cgccctggag gcaagaatta
    39241 atgctcgcgg gtctggtgga actgaacgtc cggataacgt tcctgtgcca ggcgcaggtt
    39301 gaccatgctg gtagcgatgt aagcgaggtt atcgccgcca tcaagcgcca gttggctttc
    39361 gttcttacgc ttgaactctt cgaatttctt cgcgtctgca cattctaccc agcgggcagt
    39421 ggcaacgttg actgactcat acactgcttc aacgttgtat tcgctcttca ggcgcgctac
    39481 caccacatca aactgcagca caccaaccgc accaacgatc agatcgttgt tggagattgg
    39541 acggaacacc tgcaccgcgc cctcttcgga aagctgtacc agccctttga gcagctgttt
    39601 ttgcttcagc ggatctttca ggcggatacg acggaacagt tctggtgcga agttcggaat
    39661 accggtgaac ttcatcatct caccctgggt aaaggtgtcg ccgatctgaa tggtgccgtg
    39721 gttgtgcagg ccgaggatat cgcccggata cgcttcttca acgtgcgaac ggtcacccgc
    39781 cataaaggtc agcgcgtcgg agatcaccac atctttcgca gtgcgcacct ggcgcagttt
    39841 catgcctttt tcatatttac cggacaccac acgcataaac gccacgcggt cgcggtgttt
    39901 cggatccatg ttggcctgaa ttttaaatac gaagccggta aatttatctt cgctcgcttc
    39961 tacggtacgg gtatcggtct gacgcggcat cggcgcaggc gcccactcca ccaggccatt
    40021 cagcatatga tcaacgccga agttacccag cgcagtaccg aagaataccg gagtgatttc
    40081 gcccgcaagg aacagctctt tgtcgaactc gttagacgcg cctttaacca gttccagttc
    40141 gtcacgcagc tgctgtgcca gatcttcacc aaccgcagca tcgagatccg ggttattcag
    40201 ccctttaaca atgcggactt cctgaatggt gtgcccttta ccgctctgat agagataggt
    40261 ttcatcttta taaaggtggt aaacgccttt aaacagcttg ccgcagccaa ttggccaagt
    40321 gatcggtgcg cagccaattt tcagctcgtt ctcaacttca tcgagcagtt ccatcgggtc
    40381 gcggatatca cggtcaagtt tgttcataaa ggtgaggatc ggcgtgtcgc gcagacgggt
    40441 aacttccatc agcttacggg tacgatcttc aacacctttt gcggcgtcga taaccatcag
    40501 gcagcagtcc accgccgtca gggtacgata ggtatcttcc gagaagtctt cgtgccccgg
    40561 ggtgtcgagc aggttaacca ggcaatcgtg atacggaaac tgcatcacag acgtagtaat
    40621 ggagatccca cgctgctttt ccatctccat ccagtccgac ttagcgtgct ggttggaacc
    40681 acggcctttt actgtaccgg cggtctgaat ggcctgtccg aacagcagca ccttctcggt
    40741 gatggtagtt ttaccggcgt ccgggtgaga aataatggca aaagtgcggc gcttcgccac
    40801 ctctttcaaa taaggagaca acgtcataat tctttcttct tgagtaagcg gcatcgcgcc
    40861 gcgcttgttg aaaacgaaaa attgcggcta ttttacccat caacgggggg gaggcaatca
    40921 gtgtttacac aggagctgct ccagttcgtg caacgaagaa acggtccagg tgggcgcgat
    40981 gccttctggt tgctcgcgat tgtgcgcatt cagccagcag gtcgcaagcc cggcgttgat
    41041 gccaccgaga atatcggact cggcagtgtc gccaaccatc agcacgcgtg aacggtcagg
    41101 attgcccgcc tgttccagcg cataatcgaa aattttctta ttcggtttgg caacgccaac
    41161 ttcttcggaa atcaccagca aatcgaagta atcacgcagg cccgtccgtt ccagacgcac
    41221 ttgctgcaag gcgctaaaac cgttggtgat gatgccgatt ttagcgttgc tacgaatggc
    41281 gttaagcaga gaaaccgcgc ccggcaacgg cgtacagatt tccgccatcg cattaataaa
    41341 ggcatcgtta agcaagcctg cttcaacttt cagccgttcg gcccagctct cgaaacgccc
    41401 gtgctgaagc tgtaatgaag tgatcgcgcc gttttgataa tccacccaca gtggcttgtt
    41461 aacggcctga tagtcctgaa aatcttcagc ggtaaaggtg acgctgtaat caagaaacat
    41521 ccgctgcagg ccggtgaatg agtcaaaggt aaacagcgtt tcatcggcat caaagaaaat
    41581 ccagtcccac ttcattattc caccttgtct tacatactga ttggcaacgc catgatgatg
    41641 gcgtcttcgc gaccgtccgt ggtggggtag taattgcggc gaatcgtcgc ctcgttaaag
    41701 cctaaacttt cgtacagggc aatggcggca gcatttgagg cacggacttc cagccatagt
    41761 gtcgccacgc cgcgtttttc cagttcgtcg atcagatgtt ccagcagcgc cctacccagc
    41821 ccctgacgct gatagtcggg atcgaccgca atattgaaca atgtagcttc atccagcacc
    41881 acttgcgtaa tcgcaaacgc cgccattttg ccgttttgcg ttaactgaaa gttgagataa
    41941 cgctcgccct ggttgctggc aaaggttttt tcactccacg gaaaggagtg ggcgcgttgt
    42001 tcaatgtggt aagccgctgg taaatcagtc gtttcgaggg aagaaatcgt gttcatatgt
    42061 gcaaatttgt tgccataacg cggcgcgtgc cgttgggttt gcccgtaatt cggtgagcgc
    42121 cggtgatgcc acctgagcgc cttccaatga tagcggttcg tcagtaccca accgccagct
    42181 gttgcagcga ctgccttgcg gcagcatcgc gattttttct ggcgtcagtt gcagcacttg
    42241 atcggggctg acggttaatg cgcgcagaac atcgctcact aaaggatcag tcagggcggg
    42301 aagatcgttt gccaccatca ccagacggac gtgtgccggg ctcgcaatgg caatctcccc
    42361 ctgcaacgcg ccagggcgac gcagcgacca ctgggtaatg cccagttgct gtaactgcca
    42421 gtctcgtcgg gatgtcatag cgaatcgctc ctgttgtcag gggcgcaaat atagcaaatt
    42481 cgtcgatacc gcgccaacat atggctataa tcgccgccag tatcaattga ggagcattcc
    42541 atgtctgcat ttaccccggc aagtgaagtc ttgctgcgtc acagtgatga tttcgaacaa
    42601 agccgtattc tgtttgccgg agacttacag gatgacctgc ccgcgcgttt agataccgcg
    42661 gccagccgtg ctcataccca gcaattccac cattggcagg tgttaagctg ccagatgggg
    42721 gataacgccc gttttagtct ggtcgccacg gcgaatgatg tcgcagattg cgatacgctg
    42781 atttactact ggccgaagaa caaaccggaa gcccagttcc agttgatgaa tttactttct
    42841 ctgctgccag tggggacaga tatttttgtc gttggcgaga accgcagcgg cgtgcgcagc
    42901 gctgagcaga tgctggcaga ttatgcgccg ttgaataaag tcgacagcgc tcgtcgctgt
    42961 ggcctctatt ttggtcgtct ggaaaaacag ccagtatttg atgcggataa attctggggc
    43021 gaatacagcg tcgatggcct gacggtcaaa acgctgcctg gcgtgtttag ccgcgacggt
    43081 ctggatgtcg gtagccagtt gctgctctcg acgttaactc cgcacacgaa aggtaaagtg
    43141 ctggatgtcg gctgtggcgc gggtgtactt tcagttgcct ttgcgcacca ttcgccgaaa
    43201 attcgtctca ccttgtgcga tgtctctgcc ccggcggtag aagccagccg cgcaacactt
    43261 gcagccaacg gtgttgaagg tgaagtcttt gccagcaacg tcttttccga ggtgaaaggt
    43321 tgttttgata tgatcatctc caacccgccg ttccacgatg ggatgcaaac cagcctggat
    43381 gcggcgcaaa cgctgattcg tggcgcggtg cgtcatctta atagcggcgg cgagctgcga
    43441 attgtagcga acgccttcct gccttacccg gacgtgctgg atgagacatt tggcttccat
    43501 gaagttatcg cgcaaaccgg gcgcttcaag gtatatcgcg ccattatgac ccgccaggcg
    43561 aagaaaggtt gagtatctga tatctgtcgc cgcataccgt gtcggatgcg gcgtgaacgc
    43621 cttatccgac ctacaaaacc tgcacgttag cccttcgtag gccagataag acgcgccagc
    43681 gtcgcatctg gcatctgccc ccgcatgcct accaaacctg cacgctagtt tcctgatgga
    43741 catttttcca gcaattacac atcaggcgat aattaactat tgacgaaaag ctgaaaacca
    43801 ctagaatgcg cctccgtggt agcaattctt tttaagaatt gatggtatgc gaaggtggcg
    43861 gaattggtag acgcgctagc ttcaggtgtt agtgtcctta cggacgtggg ggttcaagtc
    43921 ccccccctcg caccaaaaac cacgttgata ttgctcgcac tgggcgaagg tggcggaatt
    43981 ggtagacgcg ctagcttcag gtgttagtgt tcttacggac gtgggggttc aagtcccccc
    44041 cctcgcacca aacgaggcga tatcaaaaaa agtaagatga ctgtgcgaag gtggcggaat
    44101 tggtagacgc gctagcttca ggtgttagtg tccttacgga cgtgggggtt caagtccccc
    44161 ccctcgcacc aattatcttt acttcctttc ttgtttcttc cctgttttgt catccctgat
    44221 tcaaattcat tatcatcagc atgatgccag caataagcgc caggcacgat ggtagcaaaa
    44281 tgtaatgccg caactgtttg tgatacagca tcaccggcgt caatactaat cccatcagaa
    44341 tgacaatccg ccactgatcc ggcgataaat ccgcatacat cagccctgca atcagcaatc
    44401 ccggcagcaa cactccccag ccactgccca gcgtacgttg caacatggtt tcatctcttt
    44461 cattgataat gaaaaccaat atcatatgat aatttttatc atttgcaagc cagagaaatc
    44521 ccttgctatc gagtaaacct atcgctagga ttagcaatca ttatcattta gattactatc
    44581 acgattatgg cctatcgttc cgcaccgctc tatgaagatg tcatctggcg aacgcatctc
    44641 cagccgcagg atgctgggct tgcacaagcc gtacgggcga tgatcgccaa acatcgtgaa
    44701 catttgctgg agtttatccg cctggatgaa cccgctccgc ttaacgccat gacgctggcg
    44761 caatggtcat caccgaatgc gctaagttct ctactggcgg tctattccga tcatatctat
    44821 cgcaaccaac caacgatgat ccgcgagaac aaaccgctga tctcactatg ggcacaatgg
    44881 tatatcggcc tgatggtgcc accattaatg ctggcgctac tgacgcagga aaaagcatta
    44941 gatgtgtcgc cggaacattt ccatgctgag tttcacgaaa ccggacgcgt cgcctgtttc
    45001 tgggtcgatg tgtgtgaaga taaaaacgca acaccacatt cgccgcagca gcgaatggaa
    45061 acgttaatca gccaggcgct ggttccggtt gtgcaagcac tagaagcgac cggagagatc
    45121 aacggcaaac ttatctggag taataccggt tatttgatca actggtatct cactgagatg
    45181 aagcaactgc tcggcgaggc tactgttgaa tcgctgcgcc atgccctctt ttttgagaaa
    45241 acgctcacga acggtgaaga taatccactc tggcgtaccg tggtactgcg cgacggcctg
    45301 ctggtgcgcc gcacttgctg ccagcgttat cgcttaccgg atgtgcagca atgtggctat
    45361 tgtacgctga aatgaaagca ctgccgggga agtaaacccg gcatcatgcg gattaatagg
    45421 gatgcaacac attacttgct tcgcaatgcc gcatacataa cagaatatct gcggcctcca
    45481 acaaccctgc atcagaactg acgcccagtt tcgacatcac attaaattta tgcgctcgga
    45541 tcgttttgat attacgttta agctgctcgg caatttgtgt cattgagtag ccacgcgaca
    45601 taaagcgcaa tatttctctc tccgtcgggc ttaacgtccg gctttggtta atgtaccact
    45661 gattgttcag tcggtcggtc gcctgacgta caccatttaa tgacaaaaag agttcctgat
    45721 gaaaaatctc cagcgttgac gctttactta atacaccgtc cagcggtgat ggcgacaatg
    45781 aaccaatcag ccgagcttca atatcatcat ccacaataac taaacgccgg gtacgcggaa
    45841 acttaatcgc cagctcagtc aggcaagata atccctctct gcgctcactt ctcatggcag
    45901 aaaaagaaaa aatgaccgcc gcaaacgaga tccgcgacat ggcatcctga aatgactcct
    45961 gactggtata ggtatggagc ttataagggt tgcccgcaaa ctttctggat aaactctcaa
    46021 taccgatact actcatgacg catttttcta tgagtgcgac atttctcttc ttaattcggc
    46081 tgtgttccat tctgcggcat ctccataaac agaaggcaag ttaagttctc ttgcaccctg
    46141 cgcgctattt atccatgcat acatttcagc gttgctgtga attgccagtc gacgcatcgc
    46201 actgtttttt tgggcgctga tagttttatt acttttctta agcaatgaag ctatctggtt
    46261 gatgccccat cctttaccta atagccgcag taccttgcgc tctgaaagag tgagaatgac
    46321 ggagcgatag ctaaaatcat gagtttcagt cattgccgga gaggtcagca tggcgctgat
    46381 acgctctgcg tgcgtattgc cggaacgtac ggtcttgacc agattctcaa tgggttcaac
    46441 atcagacaat aacgtcgccg tagggcacat gagcagttct acagcctggg aataccagga
    46501 gcgtgacacc atgaaaaccc agtgaatttc ccgatattgt gagattaaag aataataatg
    46561 ttcacaaaca gaacgtggat cttcactttc accggctaaa tcagcaatga ctaat
//
LOCUS       DAEEGA010000028        45946 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEEGA010000028 DAEEGA010000000
VERSION     DAEEGA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45946
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.028"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..104)
                     /locus_tag="KLH46_001603"
     CDS             complement(<1..104)
                     /locus_tag="KLH46_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0378324.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRNLCSH"
     gene            255..2408
                     /locus_tag="KLH46_001604"
     CDS             255..2408
                     /locus_tag="KLH46_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBE0378325.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2461..4191
                     /locus_tag="KLH46_001605"
     CDS             2461..4191
                     /locus_tag="KLH46_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378326.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4251..4742)
                     /gene="yajQ"
                     /locus_tag="KLH46_001606"
     CDS             complement(4251..4742)
                     /gene="yajQ"
                     /locus_tag="KLH46_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBE0378327.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4910..5821
                     /gene="panE"
                     /locus_tag="KLH46_001607"
     CDS             4910..5821
                     /gene="panE"
                     /locus_tag="KLH46_001607"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBE0378328.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5784..6374
                     /gene="yajL"
                     /locus_tag="KLH46_001608"
     CDS             5784..6374
                     /gene="yajL"
                     /locus_tag="KLH46_001608"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBE0378329.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6428..7876)
                     /gene="thiI"
                     /locus_tag="KLH46_001609"
     CDS             complement(6428..7876)
                     /gene="thiI"
                     /locus_tag="KLH46_001609"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBE0378330.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            8082..8324
                     /gene="xseB"
                     /locus_tag="KLH46_001610"
     CDS             8082..8324
                     /gene="xseB"
                     /locus_tag="KLH46_001610"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBE0378331.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8324..9223
                     /gene="ispA"
                     /locus_tag="KLH46_001611"
     CDS             8324..9223
                     /gene="ispA"
                     /locus_tag="KLH46_001611"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBE0378332.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9248..11110
                     /gene="dxs"
                     /locus_tag="KLH46_001612"
     CDS             9248..11110
                     /gene="dxs"
                     /locus_tag="KLH46_001612"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBE0378333.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNCRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11165..12082
                     /gene="yajO"
                     /locus_tag="KLH46_001613"
     CDS             11165..12082
                     /gene="yajO"
                     /locus_tag="KLH46_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBE0378334.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12193..12711)
                     /gene="pgpA"
                     /locus_tag="KLH46_001614"
     CDS             complement(12193..12711)
                     /gene="pgpA"
                     /locus_tag="KLH46_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBE0378335.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12689..13666)
                     /gene="thiL"
                     /locus_tag="KLH46_001615"
     CDS             complement(12689..13666)
                     /gene="thiL"
                     /locus_tag="KLH46_001615"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBE0378336.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13744..14163)
                     /gene="nusB"
                     /locus_tag="KLH46_001616"
     CDS             complement(13744..14163)
                     /gene="nusB"
                     /locus_tag="KLH46_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBE0378337.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14183..14653)
                     /gene="ribE"
                     /locus_tag="KLH46_001617"
     CDS             complement(14183..14653)
                     /gene="ribE"
                     /locus_tag="KLH46_001617"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBE0378338.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14742..15845)
                     /gene="ribD"
                     /locus_tag="KLH46_001618"
     CDS             complement(14742..15845)
                     /gene="ribD"
                     /locus_tag="KLH46_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBE0378339.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15849..16298)
                     /gene="nrdR"
                     /locus_tag="KLH46_001619"
     CDS             complement(15849..16298)
                     /gene="nrdR"
                     /locus_tag="KLH46_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBE0378340.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16449..16988
                     /locus_tag="KLH46_001620"
     CDS             16449..16988
                     /locus_tag="KLH46_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBE0378341.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17286..18170
                     /gene="tsx"
                     /locus_tag="KLH46_001621"
     CDS             17286..18170
                     /gene="tsx"
                     /locus_tag="KLH46_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBE0378342.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18347..18694)
                     /locus_tag="KLH46_001622"
     CDS             complement(18347..18694)
                     /locus_tag="KLH46_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBE0378343.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18823..19794)
                     /gene="secF"
                     /locus_tag="KLH46_001623"
     CDS             complement(18823..19794)
                     /gene="secF"
                     /locus_tag="KLH46_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBE0378344.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19805..21652)
                     /gene="secD"
                     /locus_tag="KLH46_001624"
     CDS             complement(19805..21652)
                     /gene="secD"
                     /locus_tag="KLH46_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBE0378345.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21680..22012)
                     /gene="yajC"
                     /locus_tag="KLH46_001625"
     CDS             complement(21680..22012)
                     /gene="yajC"
                     /locus_tag="KLH46_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBE0378346.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(22035..23162)
                     /gene="tgt"
                     /locus_tag="KLH46_001626"
     CDS             complement(22035..23162)
                     /gene="tgt"
                     /locus_tag="KLH46_001626"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBE0378347.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23217..24287)
                     /gene="queA"
                     /locus_tag="KLH46_001627"
     CDS             complement(23217..24287)
                     /gene="queA"
                     /locus_tag="KLH46_001627"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBE0378348.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24380..24953
                     /gene="acpH"
                     /locus_tag="KLH46_001628"
                     /pseudo
     CDS             24380..24953
                     /gene="acpH"
                     /locus_tag="KLH46_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24958..26775)
                     /gene="malZ"
                     /locus_tag="KLH46_001629"
     CDS             complement(24958..26775)
                     /gene="malZ"
                     /locus_tag="KLH46_001629"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBE0378349.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26931..28304)
                     /gene="proY"
                     /locus_tag="KLH46_001630"
     CDS             complement(26931..28304)
                     /gene="proY"
                     /locus_tag="KLH46_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBE0378350.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28380..29699)
                     /gene="brnQ"
                     /locus_tag="KLH46_001631"
     CDS             complement(28380..29699)
                     /gene="brnQ"
                     /locus_tag="KLH46_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBE0378351.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(30106..31401)
                     /gene="phoR"
                     /locus_tag="KLH46_001632"
     CDS             complement(30106..31401)
                     /gene="phoR"
                     /locus_tag="KLH46_001632"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBE0378352.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31459..32148)
                     /gene="phoB"
                     /locus_tag="KLH46_001633"
                     /pseudo
     CDS             complement(31459..32148)
                     /gene="phoB"
                     /locus_tag="KLH46_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
     gene            32338..33540
                     /gene="sbcD"
                     /locus_tag="KLH46_001634"
     CDS             32338..33540
                     /gene="sbcD"
                     /locus_tag="KLH46_001634"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBE0378353.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQMDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33537..36680
                     /gene="sbcC"
                     /locus_tag="KLH46_001635"
     CDS             33537..36680
                     /gene="sbcC"
                     /locus_tag="KLH46_001635"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBE0378354.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLIVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36806..37987
                     /gene="araJ"
                     /locus_tag="KLH46_001636"
     CDS             36806..37987
                     /gene="araJ"
                     /locus_tag="KLH46_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBE0378355.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGGAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(38130..39038)
                     /gene="mak"
                     /locus_tag="KLH46_001637"
     CDS             complement(38130..39038)
                     /gene="mak"
                     /locus_tag="KLH46_001637"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBE0378356.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVTIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39163..40074
                     /gene="rdgC"
                     /locus_tag="KLH46_001638"
     CDS             39163..40074
                     /gene="rdgC"
                     /locus_tag="KLH46_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBE0378357.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40152..40607)
                     /locus_tag="KLH46_001639"
     CDS             complement(40152..40607)
                     /locus_tag="KLH46_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378358.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40604..40939)
                     /locus_tag="KLH46_001640"
                     /pseudo
     CDS             complement(40604..40939)
                     /locus_tag="KLH46_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(41094..41378)
                     /gene="ppnP"
                     /locus_tag="KLH46_001641"
     CDS             complement(41094..41378)
                     /gene="ppnP"
                     /locus_tag="KLH46_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBE0378359.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41450..42127)
                     /locus_tag="KLH46_001642"
     CDS             complement(41450..42127)
                     /locus_tag="KLH46_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBE0378360.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42388..42576)
                     /gene="yaiA"
                     /locus_tag="KLH46_001643"
     CDS             complement(42388..42576)
                     /gene="yaiA"
                     /locus_tag="KLH46_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBE0378361.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42626..43150)
                     /gene="aroL"
                     /locus_tag="KLH46_001644"
     CDS             complement(42626..43150)
                     /gene="aroL"
                     /locus_tag="KLH46_001644"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBE0378362.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43333..43791)
                     /locus_tag="KLH46_001645"
     CDS             complement(43333..43791)
                     /locus_tag="KLH46_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBE0378363.1"
                     /translation="MTIWVDADACPNVIKEILYLAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43911..44720
                     /gene="proC"
                     /locus_tag="KLH46_001646"
     CDS             43911..44720
                     /gene="proC"
                     /locus_tag="KLH46_001646"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBE0378364.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44737..45852)
                     /gene="adrA"
                     /locus_tag="KLH46_001647"
                     /gene_synonym="dgcC"
     CDS             complement(44737..45852)
                     /gene="adrA"
                     /locus_tag="KLH46_001647"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBE0378365.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11956 a        11976 c        11238 g        10776 t
ORIGIN      
        1 caatgagagc agagattgcg ctgatgtccg gcagtgcttt tgccgttacg caccacgcct
       61 tcagtagcgg agcaggaagg acatctgatg gaaatggaag ccacgcaagc accttaaaat
      121 caccatcata cactaaatca ataagttggc agcattacct tcgcttaatt ccatataact
      181 ggacgattac attagaagta aactatactt tttatgaaat atcagggata aattattcac
      241 atggaaaata catcatgtac aaatcattat ttactattat cattctactg tatagcgcca
      301 gttcatatgc aaaaagcgaa tcccctcccg caagcactat aactttcatt gcacaaatcg
      361 atcatgttga gctaaataaa acagcattat ctcatgatat gaaactcgtt cttgccgatc
      421 gtttttattt caaaaccagt aatccttgcg aaacaaattc aatttctgct cgaccagaat
      481 caatggatat tactgacaaa gcctatgccg acaaattaaa aaaattagaa actccttttc
      541 ttgatgatcg ctatttcttt atgaccatca atcagtgtga cggtaatggc actcccatgc
      601 tgacagacat aaaagtatgt acaacagaat tatgtggtgc tgaatataca aagaaaatgt
      661 ctactgtatg gctcgatgaa actttaaaac caactgctaa acgtctggca atttatttta
      721 ttcaatctcc tttaccctat gacgaggaga aaaaacaatg gaaagtcacc ggatggtata
      781 tctcatcaac tgataatcag tcttcaacta aacagattgc atttgaagga tatacagaca
      841 cagataacct ctccgattct aaatttattt cttcatttaa atcctggttt gaaagtggaa
      901 agataaaaaa cattgctaac tataatccaa aaggagaaag agatggaaaa tacgaaagtt
      961 attacaatag caatggtaac gtctatgaat ctctaagttt ttctaatggt cttatcaacg
     1021 gtgagtttat tacatattat gaaaatgggg ccattgaaag taaacgtcat tttaataacg
     1081 acaaaattaa agatggtgaa tgccctcatt tttatgaaga tgggaaaatc aaacagcaac
     1141 atagctatct gaatagtaaa ctcgatggtc ctgcatatga atattatcct gatggcaaac
     1201 tcatgcagga aaatttctat cagcaaagtg tgttgattgg aaacgatacc agttactacc
     1261 aatccggaaa aataagctct atacacaata gaaattcccg tggtcagtat gatggagcta
     1321 acgaacgtta tagtgaagag ggtaagttac tgtctaaatc cgtctataaa gacggaaaac
     1381 aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga agaggagaaa cattttgatg
     1441 agcaaggcca attaaatggt ctggttaagc aatggtataa aaatggtaat cttgcaaaat
     1501 cacaaaacta taaacatgac atactcgatg gtaacagtga agagtggtac gaaaatggca
     1561 tccctgaaag tttgtaccct tataagaatg gaaaaaccga cggcattgcc aaatcatgga
     1621 ataagtatgg taaattaacc tattcaatcg aatacaaaaa tggcgtagag aatggggcat
     1681 atcgtaactg gagcaaaaac acaggtaaac taaccaaaga aaccctgtac gtcaatggta
     1741 tcagacaggg agtggaaaaa gagtttaacg atcgcacagg taaactatta acctccacgc
     1801 aatatgttaa taataaaaga catggtacgg aggaaactta tgatcaaaac ggaataaaat
     1861 atattacatg ctatcaaaat gatcagaaat tatcgtcttt agacaatcca acacaaataa
     1921 aagataatgc aacaacaggc gattccagtg ctcaatttgc attagggaaa tacgaattca
     1981 tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa atctgcagag caaaaaaaca
     2041 ctgatgcgat atacttttta gctacagcct acaaaggcaa tggtatacct gcaaacaatg
     2101 aaaaatacct agcatatctg caacaagctg caacactggg cagtagtaat gcccaggcgg
     2161 aaattggcta tttatatctc atcggaaaag aactaccaca gaatcttcct gatgctggtg
     2221 tctggttcaa aaaagcagct gcacaaggta attttgccgc acatttctat ctgggaagga
     2281 tgtatcagaa tggtgacggc gttgagagaa atatggaaaa agccaggttt catctatcta
     2341 atgcggcaga aggtggaata gaacctgcgc ttaaagcatt aaatgaactt gaacatcaaa
     2401 caaaataaag cgctcaggga ccacgtaagg ttcatcaaac agaaaaggat atagtttgcg
     2461 atgaaagcga aatctacttt gttattaatg attttatctg ttttttattc tcatcaggtt
     2521 ttatccagcg aacccactca gccggatgtt tattctgtcg tcgagaaaaa actaaactct
     2581 gctttacctc tggaagaaaa taccaccttc agatcgcagg ctgaatggtt taatttgcaa
     2641 tatgaattat tagtatctgg ctaccctgaa cgtgcttaca atttactgag caaacttgag
     2701 acagaatcga aaaacgctaa atattatctg gatttaactg cctccagagt cgctgaagaa
     2761 gaagcaccag agacagcctt aactttttta tcaaaaattg ggttaaataa accggctact
     2821 cttacatcct ttgagagcta tatcaatagc tggataaaaa gcaaacaacc cgaaaaggct
     2881 atgcagcttc tcagcctcga tgagaatgcc ctgaattatt atttaccgac tgtgttagtc
     2941 gcatatcaga tcgttcctga taaggcaata agtatttata attctgtata tggagataat
     3001 gttgttatac cctatcagca agtcaccatg ttacttacag ttgcggaggg ttatcaaaca
     3061 aaaggcgata taaaaaacgc cattcattat gccgataagg cgctgaatat gtttgataaa
     3121 gctattgctg tagatagcag tcgtgaatcc cattatagtg atgagtattt gaaactcatg
     3181 aatatctacg ccgccaatgg caacaaagaa aaagcggtgg ttttatctca acgtctacac
     3241 aaagccatta ccgaatctca atcgaattac ttaagtactc tggaaggaat tttattgttt
     3301 tatagaaata atgatatgca gcaagactat caaaacagcc tttcaaatta catagtattt
     3361 atcgataaaa tttttgcctt ttccccatca acaagaagtg aattaagcct cattaattta
     3421 ttaaattcat taaatgaggt ggaattgatg agaaaacgtc tggcgtttct gatgacagca
     3481 ccagaatacg cttgttatga tagtcagtat tgttatgaag acaaagtcag cgcattaaaa
     3541 atcctgtatc gtcatcatga caatgagcta atggacaaat atttcacgac tcttcttaaa
     3601 gagatcagta atcaaccatt agaagactgg gatataataa taagtaatac tggtgaaaaa
     3661 tttagtgaag tgggtctaac caaatatgct caacatctcg catctttcgc tgaagaattt
     3721 tatataaaag agcaaaaatc acattctgaa agtgaaatga gaggactatt ttcatcactt
     3781 gccgaattat atagttttgg taatgatact gccagcgcaa accgagtgtt ccatcagcat
     3841 gtccctgcct tcaatacaga tcatatgatt caatatttta taaatgccaa agaatggaac
     3901 aatgccaggg aattattgat aaaagaggaa atgttgggta tgcataatct gatattgttg
     3961 gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac acattacctt tacgcttaat
     4021 aaattaacaa cacaaccagc aataacaaaa atagatagcg tgggtaatga gcaactttat
     4081 cagattggca aaatctacca taagttggaa attaaacctg agccagagca acaggtttta
     4141 attcagtcgc tttatgaccg ggcatcagga agcgcctctg caactcaata aatgcatggt
     4201 caaaagggcc accacggtgg ccctgacaat cagtagctat atccagaaag ttaatcgcgg
     4261 aagtttttga actggaacgg ctgaccgaga tcgccaccgc gcaccatcgc catgacagcc
     4321 tgcaaatcat cacgagattt gccagtcacg cggatctcat cgccctgaat ttgcgcctgc
     4381 actttcagtt tgctgtcttt gatcatcttg acgatttttt tctgagtggc gctctcaatg
     4441 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     4501 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     4561 aactgattga cctggaagtc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     4621 agctcaaatg aggcttcaac gttacggaag tcaaaacggg actccacttc gcgactcgcg
     4681 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     4741 atcttttcct ctcccttcat ttttgatgcg aagcataata cctgcaaagt taaataacca
     4801 acagtcctgc accaggacgt atcccttaat tgtgcatagt cgcaacagca tgcacaatta
     4861 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     4921 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     4981 tgaagttcag ggctggctgc gtgtaccgca accttattgt agtgtgaatc tggttgagac
     5041 agatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc tcgccaccag
     5101 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     5161 atccacactg cctgtaacta cgccaatact gttaattcac aacggcatgg gcaccatcga
     5221 agaattgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     5281 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catatcggtc cggcacggca
     5341 acaggatggc gattacagtt atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     5401 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gcgtgattaa
     5461 tccactgact gccatctgga attgcccgaa cggtgaatta cgtcatcatc cgcaagaaat
     5521 tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc gaagggcatc atacttcagc
     5581 ggaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcagaaa atatctcgtc
     5641 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     5701 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     5761 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     5821 gtgaagagac tgaagccgtc accacgatca acctcttggt tcgcggcggt atcaaagtca
     5881 ccactgccag cgtcgacagc gatggcaacc tggcaattac ctgctcgcgc ggcgtgaagc
     5941 tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga atatgacgtg atcgtgctgc
     6001 ctggtggcat taaaggcacg gagtgttttc gcgatagcac tctgctggtt gaaaccgtta
     6061 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     6121 tggtgccgca cgatatcttt ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     6181 aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     6241 tgaccagcca ggggccgggt acagctatcg actttggtct gaaaattatc gacctgctgg
     6301 ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata
     6361 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     6421 ctacaaatta cgggcgatat accttcacat tgttaaagcc ttgctcgcgc agatagagcg
     6481 cctgcagacg gctcatcacc ccgcgctcac accacagcag ccaggttttg ttctggtcga
     6541 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacatca atcccttcga
     6601 ctttcagtgg cttatcttcc tgttcatcga cagaacggat atcgagaatc acgtcgttcg
     6661 ggccgaagcc gttgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     6721 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     6781 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     6841 cgccacaata ttccggcatc gtgcgagcaa agtcttcggt gccaatctgg cgagccaggt
     6901 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgtg tcagagacgt
     6961 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     7021 tgaccagcgc ctgtacgccg taacgttcag ccactttaga tgcggcacgc accatcatac
     7081 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacaaccg
     7141 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     7201 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     7261 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     7321 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccacca ataccttcgt
     7381 agcgaccttt aatcagcagg aggcgatcgt cttccacttc cagatgaaca gttacatccg
     7441 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     7501 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     7561 ttttgccttc cagctgatcg cgatactgaa ccaacgcttt ctcaaaaata tcgtgcatgt
     7621 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     7681 cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg aacttcgatg ttatcccagt
     7741 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     7801 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     7861 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     7921 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     7981 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     8041 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     8101 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     8161 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     8221 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     8281 atgaagacgc ctctctaacc ccttttacac cggacaatga gtaatggact ttccgcagca
     8341 actcgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     8401 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     8461 cctgcgacct ttcctggttt atgccaccgg tcatatgttt ggcgttagca caaacacgct
     8521 ggacgcaccc gctgctgccg tagagtgtat ccacgcttac tcattaattc atgatgattt
     8581 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     8641 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattctaag
     8701 cgatgccaat atgccggaag tgtcggatcg cgacagaatt tcgatgattt ctgaactggc
     8761 gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag acgcggaagg
     8821 caaacacgta cctctggacg cgcttgagcg tattcatcgt cataaaaccg gcgcattgat
     8881 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     8941 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     9001 ggatgtggta ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     9061 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     9121 gatcgacgat gcccgtcagg cgctgaaaca actggctgaa cagtcactcg atacctcggc
     9181 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     9241 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     9301 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     9361 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     9421 actgaccgtg gcgctgcact atgtctacaa caccccattt gaccaattga tttgggatgt
     9481 gggacatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     9541 ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     9601 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttgctgccga
     9661 gaaagaaggc aaaaattgcc gcaccgtctg tgtgattggc gacggcgcga ttaccgctgg
     9721 catggcgttt gaagcgatga atcacgcggg cgatattcgc cctgatatgc tggtggtcct
     9781 caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg cttaacaacc atctggctca
     9841 gctgctttcc ggtaagcttt actcttcact gcgcgaaggc gggaaaaaag ttttctctgg
     9901 cgtgccgccc attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtggt
     9961 gcctggcacg ttgtttgaag agctgggatt taactacatc ggcccggtgg acggtcacga
    10021 tgtgctgggg cttatcacca cgctaaagaa catgcgcgac ctgaaaggcc cgcagttcct
    10081 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc cgatcacttt
    10141 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
    10201 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
    10261 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
    10321 attcccggat cgttacttcg acgtggctat tgccgagcaa cacgcagtga cctttgccgc
    10381 cggtctggcg attggtggat acaaacccat tgtcgcgatc tactccacct tcctgcaacg
    10441 cgcctatgat caggtgctgc atgacgtggc aattcaaaag ctaccggtcc tgttcgccat
    10501 tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac cagggcgctt ttgatctctc
    10561 ttacctgcgc tgcataccag aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
    10621 ccagatgctc tataccggct atcactataa cgatggcccg tccgcggtgc gctacccgcg
    10681 cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctaccaattg gcaaaggcat
    10741 tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt ggtacgctga tgccagaaac
    10801 ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccact
    10861 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
    10921 aaacgccatt atgggcggcg caggtagcgg agtgaacgaa gtgctgatgg cccatcgtaa
    10981 accagtaccc gtgctgaaca ttggcctgcc ggacttcttt attccgcaag gaactcagga
    11041 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
    11101 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
    11161 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
    11221 ctgtatgacc tttggcgagc cagatcgcgg taatcacgca tggacactgc cggaagagag
    11281 cagccgtccc ataattaaac gcgcgctgga aggtggcata aatttctttg ataccgccaa
    11341 tagctattcc gatggtagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
    11401 tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc gttggtgatt taccggaagg
    11461 attatcccgt gcacaaattt tgcgctctat caacgacagc ctgcgacgtc tcggcatgga
    11521 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
    11581 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
    11641 gcacgcttcg cagtttgctc aggcgctgga actacaaaaa cagcactgct gggcgcagtt
    11701 tgtcagtatg caggatcact ataatctgat ttatcgcgaa gaagagcgcg agatgctgcc
    11761 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
    11821 gacgcgtcca tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
    11881 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
    11941 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttgttaagta aaccgggcat
    12001 tgccgcaccg attatcggaa cttcgcggga agaacagctt gatgagctgc tgaacgcggt
    12061 ggatatcact ttgaagccat aacagattgc cgagctggaa acgccgtata aaccgcatgc
    12121 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
    12181 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
    12241 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
    12301 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
    12361 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
    12421 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
    12481 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
    12541 ccagggtaaa aaggtcatca gataccagaa agggatcgcc gccagcgatc ccatcgtgcc
    12601 aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact
    12661 catcttcagg cgacttttcg cgacatcttt atggcgtgac aaaatggtca tatcctttcc
    12721 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    12781 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    12841 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    12901 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca
    12961 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    13021 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    13081 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    13141 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    13201 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    13261 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    13321 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    13381 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    13441 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    13501 ggaaatggtt tcccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    13561 tattgagaag agcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    13621 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    13681 caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt ccgtgaatct accggcctgg
    13741 atattacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    13801 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    13861 tttgtatggc acatcactac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    13921 ttctacctgg cccagttctt ccagcaggcg ggacaggtag ggcttcatca gtccgtcgag
    13981 gtatgcggtg ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    14041 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    14101 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    14161 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    14221 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    14281 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    14341 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    14401 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    14461 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    14521 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    14581 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    14641 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    14701 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    14761 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    14821 aatttctcaa gccctggcag cgagcataat ccacgggcgt cgctgcctaa tagtttaggt
    14881 gcgatataga caatcagctc atcgactaaa cccgcctgta gcaatacgcc agcgagcgtt
    14941 ggccccgctt ccacccagat gctgttaatc tgctgtttac ccagttgcat catcagtaca
    15001 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    15061 tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    15121 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    15181 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    15241 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    15301 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    15361 gccgtgcggc catcaagcga tgcgccaagt tttaattgaa tataaggaaa gccggtgcgc
    15421 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    15481 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    15541 tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    15601 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    15661 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    15721 taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttgggg
    15781 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    15841 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    15901 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    15961 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    16021 gatttaatat gattgattgc catttcaacg taatcggaac tcaccggacg tttttccagc
    16081 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    16141 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    16201 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    16261 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    16321 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    16381 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    16441 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    16501 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    16561 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    16621 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    16681 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    16741 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    16801 ttgaatatgg gcaaattcag ggaacaatag acaattttca ggaaatcaat gtgcagaatc
    16861 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    16921 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    16981 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatcttccc cgtcattttg
    17041 ttactctgct tacatcacct ggattgatag taaaagtttg caacaagggc gaaagtcagt
    17101 acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg attacgtaaa tgatagaact
    17161 gtgaaacgaa acatattttt gtgagcaatg atttttataa taggctcctc tgtatacgaa
    17221 atatttagaa acgcaatttg cgcctttttc actcccgcaa gggattttca aacagtggca
    17281 tacatatgaa aaaaacatta ctggcagccg gtgcggtact ggcgctctct tcgtctttta
    17341 ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc cgactggtgg caccagagcg
    17401 ttaacgttgt cggaagctat cacacccgtt tcggaccgca gatccgcaac gatacctacc
    17461 ttgagtacga agcattcgct aaaaaagact ggttcgactt ctatggttat gcggatgcgc
    17521 cggtattctt cggcggtaac tccgatgcta aaggtatctg gaaccacggt tctccgctgt
    17581 ttatggaaat cgaaccacgt ttctccatcg acaagctgac caatactgac cttagcttcg
    17641 gtccgttcaa agagtggtac ttcgcgaaca actacattta cgacatgggt cgtaataaag
    17701 atggtcgcca gagcacctgg tacatgggtc tgggtaccga tatcgacact ggcctgccga
    17761 tgagcctgtc catgaacgtc tatgcgaaat accagtggca gaactatggc gcagcgaacg
    17821 aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt tgtgccgatt accgatctgt
    17881 ggggcggtca gctgagctac atcggcttca ccaacttcga ctggggttcc gatttagggg
    17941 atgacagcgg taacgcaatc aacggtatta agacccgtac taataactct atcgcttcca
    18001 gccatattct ggctctgaac tacgatcact ggcactactc tgtcgtagct cgttactggc
    18061 acgacggtgg tcagtggaac gacgatgcag aactgaactt cggcaacggc aacttcaacg
    18121 ttcgctctac cggctggggt ggttacctgg tagtaggtta caacttctga ttatgaaaat
    18181 gccgggattt attcccggca tttctgattg ttaacaacgt gtgctttgtt catgccggat
    18241 gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa ttcaatatat tgctgaaata
    18301 acgtaggcct gataagcgta gcgcatcagg caattttacg ttttaatcac ttcttcttgt
    18361 tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc ttcaccgaca tctttctgcg
    18421 catcttcccc tgcgataacg gtcgtaccat actgatccgc ttcggtatat ttcgaatgct
    18481 catgatcgtg gcaatagaga cacaacaatt cccagttact gccatcttcc gggttattgg
    18541 tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag gttagaataa acaaactcgc
    18601 gggaacagcg accgcagacc cacggataga tttttaatgc tttttcgcga tagccgcttt
    18661 ctaaccgtgc atagtttttt gggatgatag ccatgaaaag ttacctggat gaaatgtagg
    18721 gttacgcgca gtttcccaag atgcggggat tttcgcaatc tccatacatc aaaaaaaatc
    18781 ccgatcttct gaccgggatt ttcaacatca acgggaactt gattaaggca gaattgacgg
    18841 ctgatccgcc ccttcttttt ccactttctg ctgcaacatg tgttcgcgct tcatacccag
    18901 tttcagagcc aacgcagatg ctacgtagat ggaagatgca gtgccgatgg aaacaccgat
    18961 aagcatggtc agcgagaagc cttccagtac cggaccaccg aagagataca gcatcaggat
    19021 aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac
    19081 gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg aagttttcac gaattcggtc
    19141 cgataccacg atactgtcgt taagcgagta accgataacc gacatcaacg atgccacaat
    19201 ggtcaggtca atctcgatat ggaataacga caaaataccc agcgtaatga taacgtcgtg
    19261 cgtcagcgca ataaccaccc ctgccgccag tcgccactca aagcggaaac ctacgtacac
    19321 gaggatagac agcagcgctg ccatcaacgc catcgcaccg gtttgcgcaa ggtcagcccc
    19381 cacgctcgga ccgacgaact caatacgctt cactgctgca ttctgattgg tggattcgtt
    19441 aatcaccttc agaacttggc tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg
    19501 catacggacc atgatgtcgt ggctgctacc aaagttttgc agcattggct cttcaaaacc
    19561 ggctttttgc aatgcatcac gcattacgtc aatttcagcc ggtttttcga gcgtaatttc
    19621 gataaccgta ccaccggtga aatccagccc ccagttaaag ccgcgcacgc ccataataac
    19681 gatagcagcg attaacagca gaccagagat gccgaaagcc cagtagtccc agcgcataaa
    19741 gtcatagact ttacggccgt ggtttagttg ttcaacagta tattcctgtg ccacatcgca
    19801 ctcctcagat tgacagcttc ttgacgcgct tgccgccata tagcaggttt acgatggcac
    19861 gcgtaccgac aatcgcggta aacatcgacg tcgccacacc gataccggta gtaatcgcga
    19921 accctttaat tgccccggta cccactgcgt acaggatgat gactttaatc agcgtggtga
    19981 tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa
    20041 cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga
    20101 ccgctaccgc aagggttaag acgatacccg cgatacctgg catactcagt gttgcgcctg
    20161 gcaacagcga cataataccg acgatcaaga tcaagttggc aatcagagca ctggtcgcaa
    20221 tcagaccaaa cttcttatag aagatgatca tgaacagaat agaaaccagc agaccggcaa
    20281 ggcaagcctc cagcccctgc tcaatgttct gcatacccaa ggtcgggcca attgtacgtt
    20341 cttcaacaat ctgaatcggc gcgatcaacg caccggcacg cagcagcagt gacagctgac
    20401 gggcttcgtt cgggttgttg atgccggtga tacggaagct gttacccaga cgagactgga
    20461 tgttggcgat gttaatcacc tcttcctgtt tcaccagaac cgcacgacca tttgcatctt
    20521 tcttaccgct gtctttgtac tccacaaaca gggttgccat cggtttgccg atgttgtcct
    20581 tggtgaagtt agacataatg ttgccaccag cgctatcgag cgagatgtta acctgcggct
    20641 ggttgtattc gtcctggctg gaagtggagt cggtgatatg gtcaccagtc agaattacgc
    20701 gtttgtacag cacaaccggc tgaccttcgc gggtctgttt cacttcagag tcgcccggta
    20761 cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt taccagacgg aattccagcg
    20821 ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc ctgaataccg ggcagttcaa
    20881 caacgatacg gtcagcaccc tgacgctgaa ccaccggctc cgccacgcca agttggttta
    20941 cacggttacg caggatatta atgttctgct gcaccgcata ttcacgcgct tcactcagac
    21001 gagcatcgct cattaccgca cgcagctggt tgctgccctg gctgctaatc accaggtccg
    21061 gatggcgctt gctcagatac gcaatggctt catcacgagc tttagcatcg cggaaagtga
    21121 tgctcaggcc gtagttgttt tctttacgaa cagtggtata cgggatgccc ttttcgcgca
    21181 ggtcactgcg taggctatcg atattttgtt cctggagttt gccaagcgcg gtgtccatat
    21241 ccacttccat caggaagtga acgccgccac gcaggtcaag gccgagcttc atcggctcag
    21301 catgaatagc tgccagccag cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt
    21361 tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa ctgggtgtca gtggagtcga
    21421 agcgcgcaag aatagcgccc tcttccagtg ccacagactt agcagttatt ttttcttctt
    21481 gtaacgtttt ctggacctgg atcagcgttt gctcactggc ggcgactccg cgcgcaccag
    21541 tgatctgaac agccggatcc tcaccaaaca ggttgggaag cgcatacagc agaccgatga
    21601 caatcaccac gatcagcatg acgtacttcc acaaaggata acggtttaac acggcaattc
    21661 ccttagggaa aaattttaat tacagcgcct tcatggtgcc tttcggcagg acggcagcta
    21721 cgaagtcacg tttaataact acttcagtgg tgtcattcag cgcgatagca atgtagccgt
    21781 tttccgctac tttggttacg cgaccaacca ggccaccgtt cgtcagaact tcatcacctt
    21841 tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga
    21901 tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa aatcaaagac atcgggctac
    21961 cttgcgccgg tgcacccgtt gccgctaccg catcagaaat aaaaaagctc attaaatttc
    22021 cctcattatt aatattaatc aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa
    22081 aaatcagtta cgaagctctc taatttaccc tcttcaatag ccttgcgtaa acccgccatc
    22141 aaacgctggt agtaacgaag gttatgaatg gtgttgagtc gcgcgcctaa tatttcgttg
    22201 caacggtcga gatgatgcaa gtaagcgcgt gaataattgc gacaggtgta gcaatcacac
    22261 tcaggatcga gtgggccagt atcgctctta tatttcgcat tgcggatttt caccacgcca
    22321 tcggtcacga acaaatgacc atttcgggcg ttgcgggttg gcattacgca gtcaaacata
    22381 tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt taccaacgcc catcaggtaa
    22441 cgcggtttgt ctgccggaat ttgcggacac acatgctcca gaatacggtg catatctgct
    22501 ttcggctcac ccacagccag accgccgaca gcgtagccat caaagccgat atctaccaga
    22561 cctttaacag atatatcacg taaatcttcg taaatactgc cctgaattat accaaataac
    22621 gcatttttgt taccaagact gtcaaaacgc tcacggctac gcttcgccca acgcagagac
    22681 atctccatag agcgttttgc gtaatcccag tcagcaggat acggcgtaca ctcatcaaag
    22741 atcatgacga tatccgaacc aagatcgtac tgaatctcca tcgacttttc cgggtcgagg
    22801 aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc cctgttcggt gattttacga
    22861 atatcaccaa ggctgaagac ctggaagccg ccggagtcgg taagaatcgg tcctttccac
    22921 tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt cctggcccgg gcgcagccac
    22981 aggtggaagg tgttgccgag gataatttgc gcgccagtgg cttcaacttc ttccggcgtc
    23041 atccctttta cggtgccgta ggtgccaaca ggcataaaac aaggcgtttc cactacgcca
    23101 cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc
    23161 attttttctc cactacgtca gaaaaacagt ccaacgttta aaccagcgcc gcggaattac
    23221 tccccgacgc gctcattaat tgcctgcgga ttgtacgtga taaacatcgc atcaccgtaa
    23281 ctaaaaaagc gatatttctc ttctaccgcc gctttatagg cattcatggt gtgttgataa
    23341 ccggcaaagg ccgaaaccag cataatcagc gtcgactctg gcaagtggaa gttcgtcacc
    23401 agcgcatcga ccactttgta ctggaagccc ggatagataa agatttgggt atcgtcaaag
    23461 aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc tttccagcga acgtactgaa
    23521 gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg ccgccagtac cgcgtctacc
    23581 acatcctgcg gtacttcagc gtattccgag tgcatgatgt gatcttcaat ggtgtcgacg
    23641 cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca caaacgccat ctccacgcct
    23701 ttggcgcgta atttttccag caaaggctcg tcaaaatgca gaccagcggt cggggctgca
    23761 accgcgcccg gtttttcgct ataaacggtt tgataaagtt cgcggtcagc gtcttcgtcc
    23821 ggacgatcga tatacggcgg cagcggcata tggccgatgc tgttgaggat atccagtacc
    23881 gagcgttcat cattaaattc gacttcaaac agtgcgccgt ggcgcgcggt cattgttgcg
    23941 ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag gttttggcgc tttcgaggcg
    24001 cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct caaccagcac ttcaatcttg
    24061 ccgccgctgg ctttacgccc aaacagacgc gccgggatca cgcgggtatt attaaaaacc
    24121 agaagatcgc cggggttgag cttatcgagt aaatcggtga aagtaccgtg cgtcagcgcg
    24181 cccgtcggcc cgtccagcga cagtaaacga cagctactgc gttcaggcat gggatagtgg
    24241 gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa cgcgcatgac actgactcag
    24301 actaaaataa gagtcgggta gtctagtgcc ggggcgctct ccctgcaacc tttacccccc
    24361 tttggataga atataatcga tgaatttttt agctcacctg catttagccc atctcgcgga
    24421 aagctcgctt tctggcaatt tactggctga tttcgtacgc ggaaatcctg aagaaagttt
    24481 tccgcccgac gtcgtggctg gcattcatat gcatcgacgt attgactgac aatctgccgg
    24541 aagtccgcga agcacaggag tggtttcgta gtgaaacgcg ccgcgttgcg cctattacgc
    24601 tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg ccggactttc
    24661 cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg ccggactcac
    24721 cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg gtgcgctatc
    24781 gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc ccacgtctgg
    24841 atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc gaaacccgct
    24901 tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta taaaacgtta
    24961 gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag tcagttgccc
    25021 atgcccttct ttgcattgcc attgcacggc attgagaaac ggtgacgcgg gtagcaccac
    25081 ttcactggcc tcgccacggt tgattgccac cagtacacgt tgctgattca gcacgcggac
    25141 aaataccact acgttatctt ccgcatacag cacctgacag ccgccacgac gcagcgcctg
    25201 acttttctta cgcagcacaa tcattcgccg gtacagcgcg aataacgccg tatcctgctt
    25261 ttccacctgc caggggaacg gtttacggca aaacggatcg tttttaccat ccagtcccac
    25321 ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga ccaccgccag
    25381 cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat cgtggctatc
    25441 gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt aattatccat
    25501 ccaggccata caggtttggg cgtcaatttg ctgcggatca taagagatgt cggtattggc
    25561 aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcagcat cttccacatc
    25621 ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg cttccggctg
    25681 ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc atattattgc gcgccccacc
    25741 cgcctccccc agcatatgca ccacatccag ccgccagccg tccatattcc acggcgcttt
    25801 cagccagtgg cggacaatac tgtcttcccc gcgataaatt tcattcacca gactttccga
    25861 ctgataatcc agcttcgtca agctggcata gccaagccag tcgagcgccg tgccatcatc
    25921 actaaacgag taccagtcgc gccagggcga ttcagagttg tgacaagctc cccccgtgcc
    25981 aagattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca cgccgtccag
    26041 caccagccgc attcccagct gctgcgtatt gtgtcgcaaa cgcagcaaca ccccatcacc
    26101 gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta cgctgggagc
    26161 tttaaacacc ggattgagat acagcgctgt cacgccaagc tttttcagat acggcagttt
    26221 ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct gcgccgtgac
    26281 cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt aatagacatg
    26341 atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga tctgataaaa
    26401 aatctgatcc gcagcccatt gtgggccgat atcaggtaca tcgacggcaa actgctccag
    26461 tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc gatcgtgcca
    26521 cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa tcgccgcacg
    26581 ccatgcggtg acgcctggct gcggctgact gcgctgctta tgcattggta ctgacatttc
    26641 ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc ccgtcagcca
    26701 cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg gcaggtgcca
    26761 tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca tagcctaatg
    26821 caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg ataactttcg
    26881 gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagta ttattggttt
    26941 tcagccagct gacgatcgtg gcggcgttta aacatccagc caatcaacag cacaacaatc
    27001 cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat caacccgata
    27061 ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg cactttaaat
    27121 ttcagcgcct taacttcttc tggcggcaaa cggcgacgga aggcgatttg cgacagcagg
    27181 atcataatcc acacccatac cgtggcgaag gttgccagcg aagcgatcac caggaagacg
    27241 ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt catcaccagc
    27301 accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg tgcgctgccc
    27361 tgctctgcca taccgtggag catacggcct acgccaaata cgtcactgtt aattgccgac
    27421 agcgaagcgg tcagcacaac aaagttaaga atgctggcgg caaaggtaat gcccatatgc
    27481 tggaacgtca gtacgaacgg gctaccggca gtgccaacct gattccacgg gtagatagac
    27541 ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac ggagttaatc
    27601 gcacgcggaa tcgatttctc aggatctttc gcttcaccgg cggtaatccc gataatttcg
    27661 atcccaccgt aagcaaacat caccatttgc aacgacatta ccatgccaag ccagccgtta
    27721 ctgaagaagc cgccgttgct ccacaggtta tgaataccgg tcggttgccc gccgttgcca
    27781 atcccccaga tgatgatgcc gaaaccggcg acaatcatga tgatgatggt ggcgacttta
    27841 aagaacgaga accagaattc cagctcaccg aagaccttca cgctcatcag gtttacggcg
    27901 caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg gaacaagaca
    27961 cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat ttcaaaacag
    28021 taggtccatc cggtaatgta acctgccagc gggccaaggt tttcctgcgc ataacgcgag
    28081 aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat aatgatatac
    28141 gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat tttgatggcg
    28201 tctgccgaac cgtaaaacag cccggtgcca attgctgaac ccagtgccat aaagcgtatg
    28261 tggcgggtac ttagcccacg ctttagctta ttcttacttt ccatcaattc ctaacccatc
    28321 aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg ttcagtgatt
    28381 tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat ggccagaacc
    28441 accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg taaacgctgc
    28501 gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag aataccaaaa
    28561 agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca ccatgagcgt
    28621 gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag caccggtacg
    28681 gagatctgga tcagctggct taagccgagg ttagacacca ccatcgagaa gccgccgagg
    28741 ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc tgcacaagca
    28801 caggtcaggc caaccgccgt gaccagacag gcgatgaaga ttaacgccgc cagcaggaag
    28861 ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc gccgtttgca
    28921 gactgatcga ccagcgacgc gctgtctgaa cccagacaga acagcgccag gtacagcaaa
    28981 gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag cagacgcgct
    29041 tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac cattgcgccc
    29101 agcgtatcca tggtcagata gccgttaacg aagccgttag aaaatgcagc gttttgataa
    29161 gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcgacaac agacaggatg
    29221 accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag cagcttgccc
    29281 ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat aaacagcggc
    29341 agcgcggaat cacccgtcag cggcgcaatc cccacttcaa aggaaacggt agctgtacgc
    29401 ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag tacgccagcg
    29461 actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc cagcgccact
    29521 accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc agtccagacg
    29581 tgttcgcctg cctgtaagcc gaccattgga gggaaaataa tgttacctgc gccgacgaac
    29641 aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg atgggtcata
    29701 aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa tccctgggga
    29761 tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag gcgcggcgaa
    29821 gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg aagcgcaatt
    29881 taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta tatagcgata
    29941 agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct aatcatgcga
    30001 acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca aagaaaataa
    30061 gccagcatcg gctggcttat ggcaataaaa gatgacaaag gcggattaat cgctgttttt
    30121 ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta ctgtactctc
    30181 aatattcagg cgactttcgt gatgattcac cgcatgtttc acgatcgcta accctaatcc
    30241 gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct cggtcaggcg
    30301 cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt cggcaccgtg
    30361 cggcactcgc tgccagcgta cggtgatatg cgtgccttcc ggcgtatgat tcacggcgtt
    30421 atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca ccttgaggcc
    30481 gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag cctcgcgctc
    30541 aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg tcggcgcggc
    30601 ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct gggtctgctc
    30661 gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct catccatcat
    30721 ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca cgttggcaaa
    30781 aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg ccaccatcag
    30841 caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg tgttgagcac
    30901 cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact ccgggtaacg
    30961 cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa gaatttgttg
    31021 cgccagaccg ttacaccaga aaataccgcc ctcttccgtg gtcagcacca ccgcgtcggg
    31081 cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac ggcggcgttt
    31141 tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc taccacgccc
    31201 cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca ataaattcca
    31261 gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca ggtaaccaaa
    31321 aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca ccagcctttt
    31381 ccacgacagc cgttccagca cgtaagatac tccagttaag aaatcataag ccctgctctg
    31441 cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac cgcgcacggt
    31501 ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac gaatgtggac
    31561 atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta acagctgctc
    31621 gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa attctgtcgg
    31681 ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt cgagacttaa
    31741 tccctgcatc ttaatcacct cttccaccgc cattggcgaa atacggcgca ttaccgcttt
    31801 gattcgcgcc accagctcct tcggcgaaaa tggcttggtg atatagtcat ccgcgccggt
    31861 ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca ccactggaat
    31921 atcccgggtc atcgactcgc gcttgaggtg tttgatgaac tggataccgg agccgccagg
    31981 taacatccag tcaaggagaa ttaaatccgg ctagggttca ttcagttgat tcacagcact
    32041 gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga agcagaccat
    32101 ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga tttgccctgt
    32161 tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt tatgacagat
    32221 ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc agaaaaaagc
    32281 aaatggcaca tctgtttggg tataatcgcg accatgcttt ttcgccaggg aaccgttatg
    32341 cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa aagccgcgaa
    32401 gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca tcagatggat
    32461 gcgattattg ttgctggtga tgttttcgat accggctcgc cgcccagtta cgcccgcacg
    32521 ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt ggtactggca
    32581 ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc gttcctcaat
    32641 acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg cgacgggacg
    32701 ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc gtgacattat ttccagccag
    32761 gcggggctta acggtattga aaaacagcag catttactgg cagcgattac cgattattac
    32821 caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc catcatcgcc
    32881 acgggacatt taacgaccgt gggttccagt aaaagtgacg ccgtgcgtga catttatatt
    32941 ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat agcgctcggg
    33001 catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg cggttccccc
    33061 attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt gacattttca
    33121 aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taactcaacc catggcggtg
    33181 ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga tgtatcgcag
    33241 gagccacctg tctggctgga tatcgaaatc actactgatg agtatctgca tgatattcag
    33301 cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt acgtcggagt
    33361 cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag cgaactcagc
    33421 gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc gcagcagcaa
    33481 cgtctgcagc atcttttcac cacgacgttg cgtaccctcg ccggagaaca cgaagcatga
    33541 aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg aagattgatt
    33601 tcacccgcga gccgttcgcc agcaacgggc tgtttgccat taccggccca accggtgcgg
    33661 ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact ccgcgtctct
    33721 ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgctgaa tgtctggcgg
    33781 aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt ctggagccag aatcgggcgc
    33841 gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga gctggcgcgc tgcgccgacg
    33901 gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg ttaaccgggc
    33961 tggattacgg gcgcttcacc cgttcgatgc tgctttcgca ggggcaattt gctgccttcc
    34021 tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc actgaaatct
    34081 acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcact gagctggaga
    34141 agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg caatcgctga
    34201 cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg cagcagcaag
    34261 aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa gccagccgcc
    34321 gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct caactggcgg
    34381 cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc atcgcagaac
    34441 acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact cgcttacaga
    34501 gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca gcagaattac
    34561 agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc cgtcagtgga
    34621 acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat cgcgagcatc
    34681 tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg cttgcggcga
    34741 tcacgttgac gttaaccgcc gatgaagttg ctaccgccct ggcgcaacat gccgagcaac
    34801 gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa caaaaacgtc
    34861 tggcgcagtt aatcgtcact atccagaatg tcactctgga acagacgcaa cgtaatgtcg
    34921 cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc gatgtgaaaa
    34981 ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc ccagcgtgca cagttacagg
    35041 cgggtcagcc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag gcgtatcagg
    35101 cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa gttaaaaagc
    35161 tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag cagcttcagc
    35221 gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact caacaatggc
    35281 aagccgtcac ggccagcctc aatatcacct tgcagccaca ggacgatatt caaccgtggc
    35341 tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga catgaattac
    35401 aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa attgaacaac
    35461 gtcagcagca gctcttaacg gcattggcgg gttatgcact gacattgcca caggaagatg
    35521 aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag caacgccaga
    35581 acgaattaac cgcgctgcaa aaccgtattc agcagctgac accgattctg gaaacgttgc
    35641 cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac tggcggcagg
    35701 ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa caggatgttc
    35761 tggcggcgca aagtctgcaa aaagcccaag cgcagtttga caccgcgcta caggccagcg
    35821 tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca ctaacgcagc
    35881 tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg ctggtcactc
    35941 agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg gctctcactg
    36001 tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg cgtgaaaaca
    36061 ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac cgtcagcaac
    36121 aacaaacctt aatgcagcaa attgctcaaa tgacgcagca ggttgaggac tggggatatc
    36181 tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc caggggctga
    36241 cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac gggcgctatc
    36301 tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc tggcaggcag
    36361 atgcggtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt agtctggcgc
    36421 tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg ctgttccttg
    36481 atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat gcgctggatg
    36541 ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgtagaagcg atgaaagagc
    36601 gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct gggctacagc aaactggaaa
    36661 gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg tgaattatct
    36721 cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg tgattggtag
    36781 ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg ctctgggcac
    36841 gtttggtttg gggatggccg aatttggcat tatgagcgtg ctcacggagc tggcgcataa
    36901 cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac tggtggtggt
    36961 ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac atatcttgtt
    37021 gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt cgtcttacct
    37081 gatgctcgcc attggtcggc tggtatccgg ctttccgcat ggcgcatttt ttggcgtcgg
    37141 agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg tggcggggat
    37201 ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt atttaagtca
    37261 ggaatgctgg cgttacacct ttttattgat cgctgttttt aatattgcgg tgatggcatc
    37321 ggtctatttt tgggtgccag atattcgcga cgaggcgaaa ggaaatctgc gcgaacaatt
    37381 tcactttttg cgcagcccgg ccccgtggtt aattttcgcc gccacgatgt ttggcaacgc
    37441 aggtgtgttt gcctggttca gctacgtaaa gccatacatg atgtttattt ccggtttttc
    37501 ggaaacggcg atgaccttta ttatgatgtt agttgggcta gggatggtgc tgggaaatat
    37561 gctaagtggc aggatttcag gacgttattc accactgcgc attgcagcag tgactgactt
    37621 tatcattgta ctggcactgc tgatgctctt tttctgcggc ggcatgaaaa caacgtcgct
    37681 tatttttgct tttatttgtt gcgcgggatt atttgccctt tcagcacctc tgcaaatatt
    37741 gttactgcaa aatgccaaag gaggagagtt attaggtggc gcaggtgggc aaatagcgtt
    37801 taacctcggt agcgccgtcg gcgcatattg cggaggtatg atgctgacgc tggggctggc
    37861 atataattac gtggcgctgc ctgccgccct gctttcgttt gctgcgatgt catcgttgct
    37921 gctgtatggt cgctataagc gccagcaagc ggcggatagt ccggtgctgg cgaaaccact
    37981 ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg atgcggcgtg aacgccttat
    38041 ccgccctacg cggttctggc acattttgta ggcctgataa gacgcggcaa gcgtcgcatc
    38101 aggcatgatg cgccaattgc ctacgttttt tactcttgtg gccataacca cgcagcgccg
    38161 cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg gcgtttcaca ttcgccgccg
    38221 aagacaaatt gtttaatcaa ctgcccaacc gtttgatata aacggtctac attgctcatc
    38281 ccgcccccca ggacaatcac atccggatcg agaatattca cgacatgtgc cagcgatttt
    38341 gccagccgca gctcgtagcg acgcaatgcc agttccgcta ccggatcgct ttcttcaacc
    38401 aggcggataa tttcactgcc tttcagcgca tgtccgctca aacgacgata atccgtcgcg
    38461 aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt taccgcaata acaagggact
    38521 tcctcgcgat aacgcagttc gtcttcgtcc atccacggta gcggattgtg tccccactca
    38581 cctgccgtgc cattgccgcc gatatgcgtc cgcccattga atgccacgcc cgcgccgcat
    38641 cccgtgccga tagtcacggc aaataccgtc tgcgctcccg ctgccgcgcc atctactgct
    38701 tctgaaaccg ccagacagtt ggcatcattt gccagccgca cttcccgctg caacctcgcg
    38761 cttaagtctt tatcgaatgg ctgaccgttg agccaggttg aattgacatt cttcaccaca
    38821 ccggtgtagg gcgaaatcga acctggaatg cccataccta ccgttccgcg ctgccctgtc
    38881 gcctgctccg ccatatcaac caacgtggcg atcgtttcaa tagtctgccg gtaatcatca
    38941 cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg catcgcccag tgcaatcact
    39001 tcagttttgg tgccgcctaa atcgatacct atacgcactg tactctcctt atttttttca
    39061 atatcaatag cgtagagacg gacaaccgga ttggcaatgc aaggccgccg acaattcgtt
    39121 atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta tcatgctgtg gttcaaaaat
    39181 ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg cagaagagat ggaaaaacag
    39241 ctagcctcga tggcatttac cccatgcggc agccaggaca tggcgaagat gggctgggtt
    39301 cctccgatgg gatcgcacag cgatgcgtta acgcacgttg ccaatggtca aattgttatc
    39361 tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga ttaaacaggc gctggaagcg
    39421 aaaatcgcca aactggaagc ggaacaggcg cgtaagctga agaaaaccga aaaagattcg
    39481 ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt tcagccgttt tagccagaca
    39541 atgatgtggc tcgacacggt taacggtttg attatggtgg actgcgccag tgccaaaaaa
    39601 gcggaagata cgctggcatt actgcgtaaa agcctggggt cgttaccggt tgtaccattg
    39661 agcatggaaa acccgattga actgacgctg accgaatggg ttcgctccgg tagtgcggca
    39721 cagggcttcc agctgcttga tgaagccgag ctgaaatcgt tgctggaaga tggcggcgtg
    39781 atccgcgcga agaaacaaga tctgaccagc gaagagatca ccaatcacat tgaagccgga
    39841 aaagtggtga ctaaactggc gctcgactgg cagcagcgca ttcagtttgt gatgtgcgac
    39901 gatggttcgc tcaagcgtct taagttctgc gacgagctgc gcgatcaaaa cgaagatatc
    39961 gaccgtgaag atttcgccca gcgttttgat gccgatttca tcctgatgac tggtgaactg
    40021 gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg aagcacaacg ttaattgctg
    40081 attttccttt aatgccggat gcgacgcctg ccgcgtctta tccggcgtac gaagccacac
    40141 caagcatata attattcgct acggcgagca ataattttta gcgcagcaat attatgcgtt
    40201 ttacgccgta actgatctaa tcgcctcatc cctattcgcc tgtcagcagc acgcagaata
    40261 ttcactaacc cccatttatc gctggtaaat tgttttgtta acagctccgc ttttggggta
    40321 tcaatatatt cacgcaataa atcagaaatt gaacaaatat ccgtcaggta aggataccag
    40381 caagtttcac cgtgaaaatt acgcactcca gcgttacctg caataaagtc tttcgggtaa
    40441 tcccagatca cgtttttatc caacgtgatc caataacgcg gtaaatcggt acttccacca
    40501 ttttggctgc gcatgggata acgggtacag tgaatttgca ggttgatcgt tggcgtcagt
    40561 aagtcataga tctcccgctg taacttgctc catggacgtt gtgtcattgt ttttcctcaa
    40621 gccggtgacg cgcttcactg cgaattttcc gcgttgcccc ggttttcact aaatcacgta
    40681 actgcgaaag atcgggttat tcgacaaata agagtaatga tggatcctct tttaaccacg
    40741 ccgttttcac atcggcagcc agctgtggat tattaatagc cagcgaccga tcctgaggct
    40801 cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt gcgtaatgcc gtttggcgca
    40861 aatgatcatt gcgactttgc agcaatgcac gccaccattc atcatctgat ttatctttta
    40921 tcgtagccag ttccgcaaaa aatacctgga gatcttcact gatatttata tcaaccttga
    40981 tgaattatga atatatcgga aattgaaaaa atcgcagcaa aggaaatatt attatgggtt
    41041 aaagagagga tatttcagaa aaatgccctc ccgttcaagg ggaaggcgag gaattacaga
    41101 tagcggcaca gataagaggt aggttcggca acttgaagat gaaactcact gtgaccggga
    41161 acattaaaca ccgaaccggc ttcatacact tgccagtcgg tcgcgtcagg cagtaacaca
    41221 ttcagcgcgc cactgattac cgtcatctct tccggctcag cggtgctgaa ggtgtattcg
    41281 ccttcaacca taacacccac gctggcgcga ccagtgctgc tgctggaaaa gccgatcgat
    41341 ttcactttgc cggaaaagta ctcattactt tgaagcataa actggccctg tctggtagtt
    41401 gaacatctac ttcactatag gggccagagg cgctggctgt cacgcaaaat tacaccagta
    41461 attccgcagc cagccgtgca atcaatacgt tagagagcaa gacaggaaca tcgagctgtt
    41521 tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca atccagcatg atgacatcag
    41581 ccccttttgc cagtaattct ttcccagcat caatgatttt ttgttctgaa tcatgaatgg
    41641 ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg ccatttttgc gcctgaacgg
    41701 gcagcatctc ctcaaccgga acgataaccc ccacctgatg atcttcaaca atagaggaaa
    41761 ccagtggagg caatattcgc gacggctcaa gaaagatcgt attacgcgca gtcatactac
    41821 taatgtttgc tgtactcatt aataaaatga cgtcataacc ctgattatcg agcacttcaa
    41881 ccacaccttg cagatctcgc tccactttac gacgcgaaac atgggccagc tggttgtcat
    41941 ttaataatgt gagaatggtg tcttcgcctg cttctggcgc gtactccgcc atcacttctt
    42001 cacgacttaa cttccccagc aggctatgat gggaaatatt atcttcgtca atgtattccg
    42061 tcaggagcgg caaaacctcc tgcataggta caatgccgat ggtcaggatc gccaacgacg
    42121 cactcattgt tcatccacct tacttttctt tcgtcgttaa ttaccgggca agtgtgaagc
    42181 accactgctg acacttactt gtgctacaaa tgacaaaaag cgtagcagca gacgtgcacg
    42241 gcataatgta aagattccaa atgattccag taatggaatg ttattgttta atattctaat
    42301 tattagaaaa acataaatta tgaaaaatgt gacgcagatc ccttgtagtg gcactcagaa
    42361 tcccttccga gcagtctgat gcggtcacta tttgtcaggg tcctcatagc gttttttcgc
    42421 atccatcgct tcctgagccg tcggatgttc actgatcaac gagtctggtt taggatgatc
    42481 ggctctgagt tggtaccagg ttaccgtctg tcctggcttt cctttttcaa tcgtcactat
    42541 atatgcttca cgaggataag gcggtttcgt tggcatcgtt cttccttatt tcacgggatg
    42601 aacgttaagt ataggcgctc taaaatcaac aattgatcgt ctgtgccagg gcgctacgaa
    42661 tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat gataatatgc gcaacttcgc
    42721 gatatagcgc atcgcgttct tccagcactt cctgaacttc ttcgctcagc ggttttcccg
    42781 ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag tcggttaacc aggactgata
    42841 ctggcgcaca caaataaacc acgatcccgt tattttgcat gaagtgacga ttaaattccg
    42901 tcagaataat gccgccgcct gtagcgataa cggtggatgc cgcagttacc gcttccagcg
    42961 ccgccgtttc tctggcgcga aatcccgccc actcttccct ttcgacgatc tccgcgaccg
    43021 tcatattgag ctgtgattgc aaccactgat cggtatcgac aaaccgacgg ttaagcgaat
    43081 cggcaagggc cattccgacc gttgttttac cacagccccg aggcccgatc agaaaaagag
    43141 gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg gcgaaagccg gtgtcatgag
    43201 aatagcgatc ataccatcaa actagtacaa tttcgattgt aaagaaaaaa ttccacttaa
    43261 agtaaaaata tcaatacatc ccttactata ccaataaata ttcaagaatg aagtgtaaat
    43321 aataaattac atttagccac gactacgttg cacttccagc caccacttct ccagctccgc
    43381 ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt ccgccggtct ggatcccact
    43441 ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc tcacgaatgg tcgctggcgt
    43501 gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc tcgatggctt cagcagccaa
    43561 aggtatatct gcggtgatca ccaaatcgcc cgcttcacac tgccggacaa tttcgttatc
    43621 ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata aatcgcgatg gcggcacgcg
    43681 taaactctgg tttgctacca gtaccagcgg catctgcata cgttccgccg cgagatacaa
    43741 aatctcttta attacattgg gacacgcgtc ggcatccacc caaattgtca taaagtcatc
    43801 ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt ccggcatagt tctgtttatg
    43861 cttctgccag cgattatcaa aacaatgaat ttcacggcag gagtgaggca atggaaaaga
    43921 aaatcggttt tattggctgc ggcaatatgg gaaaagccat tctcggcggt ctgattgcca
    43981 gcggtcaggt gcttccaggg caaatctggg tatacacccc ctccccggat aaagtcgccg
    44041 ccctgcatga ccagttcggc atcaacgccg cagaatcggc gcaagaagtg gcgcaaatcg
    44101 ccgacatcat ttttgctgcc gttaaacctg gcatcatgat taaagtgctt agcgaaatca
    44161 cctccagcct gaataaagac tctctggtcg tttctattgc tgcaggtgtc acgctcgacc
    44221 agcttgcccg cgcgctgggc catgaccgga aaattatccg cgccatgccg aacactcccg
    44281 cgctggttaa tgccgggatg acctccgtaa cgccaaacgc gctggtaacc ccagaagata
    44341 ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc ggaagtaatt gctgagccga
    44401 tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc ctacgtattt atgtttatcg
    44461 aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg cgcccaggcg tataaatttg
    44521 ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga aacgggagaa catccggggg
    44581 cactgaaaga tatggtctgc tcaccgggag gcaccaccat tgaagcggta cgcgtactgg
    44641 aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac gaagtgtatg gaaaaatcag
    44701 aaaaactcag caaatcctga tgactttcgc cggacgtcag gccgccactt cggtgcggtt
    44761 acgtccggct ttctttgctt tgtaaagcgc caaatctgcc gatttcaacc actcacgata
    44821 gtgactcatt tgtgggttca gcggcgcaac ccccacacta atccgtaaag ttacctgtgg
    44881 cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc cgtaacatgg cggtaatggc
    44941 gctctcagct ggcgtaccgg acatgattac tgcaaactca tcgccgccaa accgaccaat
    45001 cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc
    45061 gcccacatca tgaccccagg tatcgttgat gctcttgaaa tggtcgatat cgataatcag
    45121 taacgttgca tcgcgattat gccgccgaca gttatcaaat tcattgcgta acatagtttc
    45181 ccaatgacgt cggttataca cgcccgtcat gccgtcgcga gtactcatga cctgcaacct
    45241 gcgtttatgt tccgccagtt tggttgccgt ctggtagctg acccagccaa acatcagagg
    45301 ataaacgaca ataatgggaa gggagagcca ccattccagc ggagcactat tgaacgacac
    45361 ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc accatcaaca ccagacccgc
    45421 gacaaacaga cgggggccgc ctgcccccat caaattcaga cacagaatca tcaacatagc
    45481 ggtggaaggc agcacgttta cgcccattac gcctacccac attcccgcta atactgcatc
    45541 ggtttttaag ttgtaaattt cccggctaag cggatcgacg gccctgctcg ctatctgcca
    45601 ggctaaatgc ggccagacga acgcccagcc gaccaacacc agccaccacc agcccggcgg
    45661 cgggtgcgaa accagcgttg aagcaatcgg taagaacatg ccagccaggc caaccgcacg
    45721 gggtagtctg acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt
    45781 taaaggaggt tcctccccat gcgccgccgc ttttttgaaa aagttttcat cattcattat
    45841 ttttgggaac attcagaaac aattttccca aattatagag acggggctaa aggcagaagt
    45901 atgaaatttc gggtgagcca ctttcgtagc tcacccgtta gcacta
//
LOCUS       DAEEGA010000029        39815 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEEGA010000029 DAEEGA010000000
VERSION     DAEEGA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39815
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.029"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            64..225
                     /locus_tag="KLH46_001648"
                     /pseudo
     CDS             64..225
                     /locus_tag="KLH46_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            454..1044
                     /gene="uidR"
                     /locus_tag="KLH46_001649"
     CDS             454..1044
                     /gene="uidR"
                     /locus_tag="KLH46_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBE0378366.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1432..3105
                     /gene="uidA"
                     /locus_tag="KLH46_001650"
     CDS             1432..3105
                     /gene="uidA"
                     /locus_tag="KLH46_001650"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBE0378367.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWGDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATS"
     gene            3141..4166
                     /gene="uidB"
                     /locus_tag="KLH46_001651"
                     /pseudo
     CDS             3141..4166
                     /gene="uidB"
                     /locus_tag="KLH46_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4205..5470
                     /gene="uidC"
                     /locus_tag="KLH46_001652"
     CDS             4205..5470
                     /gene="uidC"
                     /locus_tag="KLH46_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBE0378368.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5696..7204)
                     /locus_tag="KLH46_001653"
     CDS             complement(5696..7204)
                     /locus_tag="KLH46_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBE0378369.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7305..8480)
                     /gene="manA"
                     /locus_tag="KLH46_001654"
     CDS             complement(7305..8480)
                     /gene="manA"
                     /locus_tag="KLH46_001654"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBE0378370.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8679..10325
                     /gene="fumB"
                     /locus_tag="KLH46_001655"
     CDS             8679..10325
                     /gene="fumB"
                     /locus_tag="KLH46_001655"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBE0378371.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10468..11871
                     /gene="fumC"
                     /locus_tag="KLH46_001656"
     CDS             10468..11871
                     /gene="fumC"
                     /locus_tag="KLH46_001656"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBE0378372.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11868..12797)
                     /gene="tus"
                     /locus_tag="KLH46_001657"
     CDS             complement(11868..12797)
                     /gene="tus"
                     /locus_tag="KLH46_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBE0378373.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12873..14174)
                     /gene="rstB"
                     /locus_tag="KLH46_001658"
     CDS             complement(12873..14174)
                     /gene="rstB"
                     /locus_tag="KLH46_001658"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBE0378374.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14353..15672)
                     /locus_tag="KLH46_001659"
     CDS             complement(14353..15672)
                     /locus_tag="KLH46_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBE0378375.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15897..16640)
                     /gene="rstA"
                     /locus_tag="KLH46_001660"
     CDS             complement(15897..16640)
                     /gene="rstA"
                     /locus_tag="KLH46_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBE0378376.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16769..17104
                     /locus_tag="KLH46_001661"
     CDS             16769..17104
                     /locus_tag="KLH46_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBE0378377.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17101..17823)
                     /gene="folM"
                     /locus_tag="KLH46_001662"
     CDS             complement(17101..17823)
                     /gene="folM"
                     /locus_tag="KLH46_001662"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBE0378378.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17860..19242)
                     /locus_tag="KLH46_001663"
     CDS             complement(17860..19242)
                     /locus_tag="KLH46_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE0378379.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19428..20372)
                     /locus_tag="KLH46_001664"
     CDS             complement(19428..20372)
                     /locus_tag="KLH46_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE0378380.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20896..22428
                     /gene="pntA"
                     /locus_tag="KLH46_001665"
     CDS             20896..22428
                     /gene="pntA"
                     /locus_tag="KLH46_001665"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBE0378381.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22439..23827
                     /gene="pntB"
                     /locus_tag="KLH46_001666"
     CDS             22439..23827
                     /gene="pntB"
                     /locus_tag="KLH46_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBE0378382.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23852..24886)
                     /gene="tqsA"
                     /locus_tag="KLH46_001667"
     CDS             complement(23852..24886)
                     /gene="tqsA"
                     /locus_tag="KLH46_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBE0378383.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25297..25662
                     /gene="mdtJ"
                     /locus_tag="KLH46_001668"
     CDS             25297..25662
                     /gene="mdtJ"
                     /locus_tag="KLH46_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBE0378384.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25649..25978
                     /gene="mdtI"
                     /locus_tag="KLH46_001669"
     CDS             25649..25978
                     /gene="mdtI"
                     /locus_tag="KLH46_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBE0378385.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(26017..26838)
                     /locus_tag="KLH46_001670"
     CDS             complement(26017..26838)
                     /locus_tag="KLH46_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBE0378386.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26938..27021)
                     /locus_tag="KLH46_001671"
     CDS             complement(26938..27021)
                     /locus_tag="KLH46_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378387.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27114..27449)
                     /locus_tag="KLH46_001672"
     CDS             complement(27114..27449)
                     /locus_tag="KLH46_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBE0378388.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(28017..28274)
                     /locus_tag="KLH46_001673"
     CDS             complement(28017..28274)
                     /locus_tag="KLH46_001673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378389.1"
                     /translation="MVGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMPFGLL
                     MTLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKG"
     gene            complement(28290..29099)
                     /locus_tag="KLH46_001674"
     CDS             complement(28290..29099)
                     /locus_tag="KLH46_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0378390.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSP"
     gene            29206..30108
                     /locus_tag="KLH46_001675"
     CDS             29206..30108
                     /locus_tag="KLH46_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0378391.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30243..31463
                     /gene="mlc"
                     /locus_tag="KLH46_001676"
                     /gene_synonym="dgsA"
     CDS             30243..31463
                     /gene="mlc"
                     /locus_tag="KLH46_001676"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBE0378392.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31588..32283
                     /gene="bioD"
                     /locus_tag="KLH46_001677"
     CDS             31588..32283
                     /gene="bioD"
                     /locus_tag="KLH46_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBE0378393.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32236..33528)
                     /gene="clcB"
                     /locus_tag="KLH46_001678"
     CDS             complement(32236..33528)
                     /gene="clcB"
                     /locus_tag="KLH46_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBE0378394.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALVG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33687..34301)
                     /gene="dmsD"
                     /locus_tag="KLH46_001679"
     CDS             complement(33687..34301)
                     /gene="dmsD"
                     /locus_tag="KLH46_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBE0378395.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34344..35198)
                     /locus_tag="KLH46_001680"
     CDS             complement(34344..35198)
                     /locus_tag="KLH46_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBE0378396.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35200..35817)
                     /gene="dmsB"
                     /locus_tag="KLH46_001681"
     CDS             complement(35200..35817)
                     /gene="dmsB"
                     /locus_tag="KLH46_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBE0378397.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35828..37866)
                     /locus_tag="KLH46_001682"
                     /pseudo
     CDS             complement(35828..37866)
                     /locus_tag="KLH46_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37927..39036)
                     /locus_tag="KLH46_001683"
     CDS             complement(37927..39036)
                     /locus_tag="KLH46_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBE0378398.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLADLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            39019..>39815
                     /locus_tag="KLH46_001684"
     CDS             39019..>39815
                     /locus_tag="KLH46_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBE0378399.1"
                     /translation="MTDINHDDTAYPSSSFSGSPYLYQQVYTSSPDTRLTQIKDLATP
                     QLVRRTNGRYTVRFRLKGATSPFLSVSTRSTDRRTATMRQRELAATAKAFLLDNPEVT
                     LQELTAHIRSMAEQFLTDASDDYWNGVEVATLVDEKSNLRELAATRALSVDQQRGIKL
                     ALEVLTAAQQRVDTGDTSGLIKLIDGGVSQLTDDNNHTDYSTIGDSTSILKNEQGDRP
                     AVFTQERQLLTPESRESSVLFQELVTSLLAEKVQTLKQSSFKDLSASL"
BASE COUNT        10144 a         9649 c        10188 g         9834 t
ORIGIN      
        1 gcatgacaag gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat
       61 tacccagaaa ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg
      121 caagatattg ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga
      181 caaattctca ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg
      241 gtaaacggtc gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct
      301 aaacaactgg cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg
      361 aatagagtgt accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt
      421 aaactagtta ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca
      481 caaccgacac ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt
      541 cacagtgcct cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat
      601 caccatttca tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg
      661 gcgctggctc gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag
      721 tctattgtct ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg
      781 gcggaaggga tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc
      841 actgaatttg ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac
      901 atcaacacgg caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat
      961 atcgaagcgg aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt
     1021 ggcggtatat taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg
     1081 cgtttcatat cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc
     1141 cccaaaatat tcaccgtagc catatgtcat gagagtttat cgttcccaat actctcgaac
     1201 gaacgttcga ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc
     1261 catatcacgc tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta
     1321 tttccatttc tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa
     1381 ctaatcagat taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt
     1441 cctgtagaaa ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg
     1501 gatcgcgaaa actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg
     1561 gcaattgcag tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat
     1621 gtgggcaacg tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt
     1681 atcgtgctgc gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa
     1741 gtgatggagc atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt
     1801 gccgggaaaa gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc
     1861 ccgccgggaa tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat
     1921 ttctttaact atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg
     1981 ggggacgata tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac
     2041 tggcaggtgg tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg
     2101 gttgcaactg gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa
     2161 ccgggtgaag gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat
     2221 atctacccgc tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt
     2281 aaccacaaac cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc
     2341 aaaggattcg ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc
     2401 aactcctacc gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa
     2461 catggcatcg tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt
     2521 ggtttcgaag cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa
     2581 actcagcagg cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca
     2641 agcgtggtga tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa
     2701 tatttcgcgc cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc
     2761 gtcaatgtaa tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg
     2821 tgcctgaacc gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag
     2881 gtactggaaa aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc
     2941 gaatacggcg tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa
     3001 gagtatcagt gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc
     3061 gtcggtgaac aggtatggaa tttcgccgat tttgcgacct cgtaaggcat attgcgcgtt
     3121 ggcggtaaca attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt
     3181 atggttcact tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg
     3241 ctcgtgggat tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca
     3301 ttaagaactc cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg
     3361 tgattgccgg aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac
     3421 gtatcgtggc gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc
     3481 tgtttatgtt gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct
     3541 cgtcgttgtt ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac
     3601 tggtgcaaaa cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga
     3661 ggatcggtaa aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc
     3721 tattcttctg ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt
     3781 caattggtca gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat
     3841 acggtgaata cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta
     3901 cccgtaaatg cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg
     3961 gatatatcgc caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg
     4021 ccttagtacc ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca
     4081 cggataaaaa attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc
     4141 aattaatcag cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc
     4201 aactatgaga aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac
     4261 ctctgcttac gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa
     4321 cgaattgcgc agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt
     4381 acagggagga ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg
     4441 cggagcgtat tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct
     4501 tgatggtgat aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa
     4561 tagcctgctt aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg
     4621 tattccgtta atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt
     4681 aggttattgg gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt
     4741 tttatggact gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg
     4801 aaaatacgat aaacatcgcg cacgttcttt tttagccgcc agttggcatg atgataccag
     4861 tcgctattct ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct
     4921 cgagaaaagc ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta
     4981 cgcgcagctc gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag
     5041 tggacaattg acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg
     5101 ccatgcaatg aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct
     5161 tgatcgtaac cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc
     5221 gcaatttacg ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga
     5281 tgtgcggatt gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc
     5341 gttacaaggg atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag
     5401 tacgctgggc gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat
     5461 gctgaagtaa gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag
     5521 tgcgcgtttt ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa
     5581 attcaaagtg ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg
     5641 tttgttttca gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact
     5701 gctgcggaat tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa
     5761 aatcttctaa cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc
     5821 tggtggtgat ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg
     5881 caccttcaac ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct
     5941 tcgctacctg agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca
     6001 gagatttaac cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg
     6061 ccggatcttt caggaacagc gacagattca gcgcactttc accctgactg tttttccagc
     6121 ttagcggcgc gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga
     6181 aggcttccgt cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca
     6241 gcagcgcctg agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa
     6301 tctggccgac ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc
     6361 tgtttagcga gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt
     6421 taccgctgat ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca
     6481 ttttttccag tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg
     6541 aaccgtcggt tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg
     6601 cgtctatccg accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag
     6661 catttaactg gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca
     6721 gtggattgag cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct
     6781 caaaaggcgt ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca
     6841 gcgtggtttg aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg
     6901 ggaagggacc atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg
     6961 gattttcttt cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc
     7021 gatgatagtt ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg
     7081 cgttcgcctg cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg
     7141 cgccgcctgt ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat
     7201 tcataatgat tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc
     7261 ctttaataag cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa
     7321 acacgcgcta aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac
     7381 gctgattcac ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct
     7441 tcgacgcaga acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg
     7501 tcatgcagcg agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt
     7561 ttcaccggct gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc
     7621 agttccggaa tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc
     7681 gccatcactt ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac
     7741 atcgcttcgc caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg
     7801 tcttccgggt aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg
     7861 tcgagcgccg attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac
     7921 aggttggcga acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc
     7981 gccggatgtg caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac
     8041 gcgttcatcg caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct
     8101 ttatagttac gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg
     8161 atttcagaat tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat
     8221 actttgaaca ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc
     8281 gatttatcac tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact
     8341 cgtgaactgc ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga
     8401 ttttccatac cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact
     8461 gagttaatga gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt
     8521 aaagcaagaa tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt
     8581 ttgttaaaaa actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc
     8641 ccagagcata accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt
     8701 atcagtctcc ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac
     8761 acgttagcgt atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa
     8821 ctctgttggc gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac
     8881 aacaggtggc cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc
     8941 tgcaattcct gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata
     9001 ccggcaccgc gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg
     9061 aagcggcgct ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc
     9121 aaaacgcgcc gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga
     9181 tcgatcttta tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg
     9241 gttcggcaaa caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac
     9301 tgaaaaatta cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc
     9361 atattgcgtt cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg
     9421 cttccgcgaa atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc
     9481 gcgatgtgga actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc
     9541 agtttggtgg taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg
     9601 catcctgtcc ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga
     9661 tcaaccgtca ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg
     9721 aagagctgcg taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga
     9781 aagagatcct cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca
     9841 cgattatcgt tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg
     9901 aagggctgcc gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc
     9961 cggaaggtta tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg
    10021 tcgatcaact gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc
    10081 agcaggtgac ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc
    10141 cggccgctgt attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac
    10201 tgggaatgga agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg
    10261 atgataaagg aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga
    10321 aataaacaga gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg
    10381 ttatcaaata gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga
    10441 aaaaattaat caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg
    10501 ggggcgattg atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag
    10561 catttccgca tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc
    10621 aagcgcgcag cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc
    10681 gccattcggc aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg
    10741 gctatctggc agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct
    10801 aaccgggcca gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac
    10861 gacgacgtga acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg
    10921 gcgctgctgg cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg
    10981 agtgaaaaat cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac
    11041 gccacgccgc taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat
    11101 ctcaaacata tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg
    11161 gtgggtactg gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca
    11221 gtcattacct gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt
    11281 gatgccctgg ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc
    11341 gccaatgatg tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc
    11401 ccggaaaatg agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa
    11461 gcattaacca tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc
    11521 gcttccggta actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa
    11581 tcggtgcgct tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt
    11641 gaaccgaatc gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg
    11701 cttaacaccc acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa
    11761 gggctgacct taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac
    11821 agctgggtac ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca
    11881 tacaggtgca gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc
    11941 tgcacattgt cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta
    12001 tcccgattaa tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct
    12061 cctttgtacc agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc
    12121 gcgttctgtg gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc
    12181 tcgcgcgtcc acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg
    12241 acttcatcac gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg
    12301 gcggggtcgt gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa
    12361 tgacgatgca cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata
    12421 tgctcgaacg tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc
    12481 gaaaggttat cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg
    12541 cgattttcgg actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc
    12601 gcgtcgttgc cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct
    12661 ttttttacct ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga
    12721 gcggcaaatg tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg
    12781 agatcgtaac gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca
    12841 gtcgtggtgg cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta
    12901 tgccataacg gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta
    12961 accgtaccgc ccattgccag tgctattgag tggacaattg ccagccccag cccgcagccg
    13021 ccggttgagc gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg
    13081 ttttctgggg caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac
    13141 agtagcaggc tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta
    13201 tccagcacgc gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt
    13261 tttatccgta ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc
    13321 cacaacggca ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca
    13381 taagtcagca gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg
    13441 gattcggcgg cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt
    13501 gttcgcagtt cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg
    13561 atattgtccg ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca
    13621 aagtggatac gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt
    13681 aacatatcct gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca
    13741 ataaaagcga tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga
    13801 acaggaccaa ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga
    13861 tcgtccaggg cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg
    13921 tatttactca gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc
    13981 gtcttacccc agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa
    14041 ctgttcatca aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc
    14101 agcccaacca gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata
    14161 aacaattttt tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt
    14221 ggagcaatgt aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga
    14281 cggatgtatc cccggtgtat tcatattcag taaattcaat tttacaccgg ggcttgttta
    14341 ttatgctaag gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag
    14401 aagacgggct gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc
    14461 catatcaacg atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag
    14521 tgttgaaagg acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat
    14581 tgagccaagc gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat
    14641 acccattgtt ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt
    14701 ttgtcccatg ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa
    14761 tttttccaat gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc
    14821 aaaggtaagt acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag
    14881 caaaatgccc gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa
    14941 cccgagcaaa ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc
    15001 cattacccca taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg
    15061 aatcgcgttg atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt
    15121 taacagcgca ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct
    15181 tgccccggtc agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa
    15241 agataccgca gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa
    15301 ggcgctggca tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat
    15361 gactaaacac aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt
    15421 ttcgagctta ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt
    15481 taataaatta acataaccgt taccagcaat attataccat tcgataacgg tttcactgct
    15541 tgtctcagga atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa
    15601 aacacttttg gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat
    15661 caaaaaaagc acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca
    15721 gcacctgaaa tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg
    15781 aggcgattat agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg
    15841 aataagctct tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag
    15901 tcatctgatt gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta
    15961 cgcacagttt taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc
    16021 gaaatagcca cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc
    16081 agcaatgcat cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg
    16141 aaatcagctg tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg
    16201 tcgatagtta acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga
    16261 cctttggtca gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa
    16321 acagcagggg gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg
    16381 tggttcatat cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt
    16441 aaatcacgac aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa
    16501 tccggatttt ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc
    16561 tgcatatcat gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc
    16621 acaaatacga tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg
    16681 ctgtttttac ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga
    16741 cttgttatgg aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc
    16801 gctggtagtg ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct
    16861 gattccactt ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg
    16921 cattgaagcc ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt
    16981 ttacctggtg tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg
    17041 ttctgttgcg tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca
    17101 ttaacgcaga tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt
    17161 aagtaagtaa tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga
    17221 tttattcagc gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga
    17281 tggcgcaata gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat
    17341 attatccagt gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac
    17401 cacataatcg gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct
    17461 ttccagcgca tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag
    17521 tacgtcggcc agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat
    17581 agcacgcaga ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt
    17641 ggtcgaaaaa tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg
    17701 atagtgtgtc cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag
    17761 ggcgaggccg atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc
    17821 catcgttatc tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt
    17881 cgccggaact gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac
    17941 attatcatgt gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag
    18001 aacaacggat aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat
    18061 gcaggcaccg acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc
    18121 gccgaccagg aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta
    18181 atcggaaccg gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca
    18241 cagtgaggcg gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt
    18301 atgagtggca gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc
    18361 gcaaacggaa acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc
    18421 caccatcaga ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac
    18481 acccagcgaa agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag
    18541 tgtcgctttg ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc
    18601 cacaccaatg aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg
    18661 tacgccaagt gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat
    18721 cgccagcaca acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat
    18781 gctggcagcg gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga
    18841 tgcaccgact atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc
    18901 cgtaaaaaag cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc
    18961 gcacagccag tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc
    19021 ataggtaaag ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa
    19081 ggccagcaat aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct
    19141 ggcaactgcc gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa
    19201 taccagcgcg gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa
    19261 acgtgataag aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag
    19321 cgcgggattt tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa
    19381 aaatgcgaaa ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga
    19441 tctgcgctga cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac
    19501 ttagcacctt tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg
    19561 tagttgccag agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc
    19621 agttcttcga ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac
    19681 tgggtttcaa agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg
    19741 atctcaactt tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa
    19801 tctgccggga tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg
    19861 aatgcagtaa tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa
    19921 gagtaggcac ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta
    19981 cgatagaagc cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc
    20041 ggcagttcga caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg
    20101 gctttataga ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg
    20161 acaacataaa aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct
    20221 tcgccaatag cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc
    20281 gcttgctccg gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt
    20341 gccgacgcca ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc
    20401 aggtactggt attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac
    20461 tgtctgcgtc atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc
    20521 gtctatgctt aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata
    20581 agttaattta atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt
    20641 cgaagacaat tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg
    20701 aatcgttaat attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc
    20761 ataattttca gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta
    20821 gctcgtacat gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg
    20881 atggaaggga atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt
    20941 gttgcagcaa cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta
    21001 gagagcggcg cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct
    21061 gaaattgtag aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg
    21121 ttagatgatg aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct
    21181 gcgcagaatc cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg
    21241 gactctgtgc cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat
    21301 atcgccggtt atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg
    21361 caaatcactg cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt
    21421 gcaggtttgg ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac
    21481 acccgcccgg aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat
    21541 tttaaagagg aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc
    21601 aaagcggaaa tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc
    21661 gcgcttattc caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg
    21721 aaggcgggca gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc
    21781 gtgccgggtg aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt
    21841 ccgggccgtc tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg
    21901 aaactgttgt gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt
    21961 cgcggcgtga ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta
    22021 tcagctcagc cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt
    22081 gcctgctcac cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg
    22141 ctgtcaagcg ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc
    22201 gttgtcggtt attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg
    22261 gttaccaacg cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga
    22321 ggctgggtta gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt
    22381 ggcttcaccg tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat
    22441 gtctggagga ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct
    22501 ggccggtctt tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat
    22561 ggcgattgcg ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt
    22621 gctggcgatg gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac
    22681 cgaaatgcca gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt
    22741 tggctttaac agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca
    22801 cctgacggaa gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt
    22861 ggcgttcggc aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca
    22921 caaaatgaac ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac
    22981 ggacagcgtc ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt
    23041 cggctggcat ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct
    23101 gaactcgtac tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct
    23161 gattgtgacc ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa
    23221 ggcgatgaac cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc
    23281 tactggcgat gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga
    23341 actgctgaaa aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca
    23401 ggcgcaatat cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg
    23461 tttcggtatc cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga
    23521 agcaaaagta ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga
    23581 taccgatacc gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga
    23641 tccgaagagt ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat
    23701 tgtctttaaa cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa
    23761 ggaaaacacc cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc
    23821 tctgtaaccc tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa
    23881 cggcgatgct ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg
    23941 gcacggaaag cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa
    24001 ataccaccaa tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc
    24061 cgaagaccag attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat
    24121 taaacaccag tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga
    24181 tgtaattaag cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg
    24241 cgagcatcgc ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat
    24301 aatgagaaac actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca
    24361 tcatttgctg aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca
    24421 gcagcaataa aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca
    24481 acgtcatcgc cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc
    24541 ctacgcgttg caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt
    24601 gcggtaacgt ccacgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca
    24661 tcgccatcac gatgattgtc atcaaaatcg acactgccag tacacgtggc acacgccagc
    24721 ggaccatatg ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg
    24781 gcacgatgat ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca
    24841 taatgacgat ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt
    24901 ttcttccttt gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg
    24961 ctcaggtaaa gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg
    25021 tgttcaacta ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt
    25081 gtaatattat ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct
    25141 catccactat cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc
    25201 tgcctaagct tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag
    25261 cgaaaattaa aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta
    25321 ggtctggcta ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt
    25381 gagggaaatg gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc
    25441 tctttcgccg ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt
    25501 attttattta ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa
    25561 attgccgggt taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa
    25621 gcgcgtaaac ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg
    25681 gctggcattg gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg
    25741 ctttcgtcgc aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc
    25801 gctttctcag gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt
    25861 tggtattgcc gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa
    25921 aggctggatt ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg
    25981 aagacgctgc ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag
    26041 cttgtcgcga aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc
    26101 ggcaggagcc gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag
    26161 aggcgaaccg ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc
    26221 ccagccagtc acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta
    26281 tcccgcctga gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc
    26341 tccctcaaat aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc
    26401 gaagtcccac ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg
    26461 ctttcccagt gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc
    26521 tttatttgac acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa
    26581 taaacagtgt cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt
    26641 gccgctggcc gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac
    26701 tggcacacga tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc
    26761 aacgtctggt ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac
    26821 agcaatggtt gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc
    26881 tcataaatac tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca
    26941 ggaaagataa aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc
    27001 aaagcgataa cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct
    27061 tagcaccgta attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct
    27121 gcgggttgtg cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc
    27181 gctgcctgcg ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt
    27241 ttagccgcct gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt
    27301 ttcgctggcg ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca
    27361 gcaaaggcgg cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc
    27421 attgtcatac cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact
    27481 atatccctgg gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac
    27541 aaacgctgaa taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct
    27601 accaataata gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa
    27661 ctgcgcatca gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa
    27721 tcctgatgac gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc
    27781 tattaacgtt ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt
    27841 atttttcagg cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc
    27901 atcagagcaa taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc
    27961 agcgtcccgg caatactcga ccccagatag taactgaaca gatacagcga ggaggcctag
    28021 cctttagcgc gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag
    28081 aatccggctg agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc
    28141 atcagtaaac caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag
    28201 cgggtggtca tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat
    28261 aataagccaa ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg
    28321 taattaaaca gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt
    28381 aatccccggt cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa
    28441 gtcgggcaaa aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc
    28501 gcgaaacaac cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca
    28561 ctaattaagc gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa
    28621 aaggccacga aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg
    28681 ccacttaacg aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc
    28741 attgtcgaaa gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg
    28801 cgaccaatgg catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg
    28861 gaaatggaca gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc
    28921 ggaaggatag gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc
    28981 gccagggtga cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa
    29041 atgctttgct tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact
    29101 tgaaatcctt gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat
    29161 tgttctgtct aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt
    29221 cgtcatctgc gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc
    29281 cgcctgaata tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc
    29341 ggtgcccgac tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag
    29401 tttcttgcag atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa
    29461 agactgcatc agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt
    29521 attcgggcgg tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta
    29581 caaacccgcg aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat
    29641 atgggattgc tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat
    29701 gaaccgctta tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca
    29761 ctggctgaac tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg
    29821 actgggctgt atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc
    29881 actcaggagg tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt
    29941 tcaattttgc ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt
    30001 gctgaagagg atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt
    30061 ccagctgcgc gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag
    30121 cgaaaaagcc cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt
    30181 attgaagtgc ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg
    30241 cggtggttgc tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg
    30301 cggtttatcg cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg
    30361 cgcagctggc tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg
    30421 tgcgagagct ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg
    30481 ttgaaactga agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg
    30541 ctctgcgcga tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag
    30601 atgacttgcc attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc
    30661 agaaaaaact tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg
    30721 aaaatggtat tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg
    30781 aggcgctgga gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga
    30841 cgatggcaga ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta
    30901 tcgatcacaa cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca
    30961 gtagtctcgt ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg
    31021 ggaaccacgg ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc
    31081 tgcgtctcaa tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat
    31141 tgtgtcaggc ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg
    31201 cgcatgtagg gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga
    31261 ttggctcacc gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc
    31321 gtcagcaggc ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta
    31381 accagggcac gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt
    31441 tgattcgtct gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc
    31501 aatttgggcc aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat
    31561 ttgtgaagca taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac
    31621 acttctgtag ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga
    31681 aaaagggttg cgggatataa acccgtagcg aaggggagca aagagacacc cgaagggctg
    31741 cgtaataaag atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca
    31801 gttaatccta tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat
    31861 tacaccctca tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta
    31921 gaagggactg gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta
    31981 gtgcaggaac aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat
    32041 gcactgctaa cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct
    32101 aaccgaatca acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa
    32161 cttccggcac cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg
    32221 gggcaataca tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg
    32281 taacgtccgc gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg
    32341 atactccccg gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc
    32401 cgccaacagt gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg
    32461 gaaccataat cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt
    32521 cggtgtaaag acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt
    32581 acaaaggaag atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac
    32641 ggtgctatag ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa
    32701 cccgactagt gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca
    32761 ggcgttcatt aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat
    32821 aatcagcgca tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc
    32881 gtcgctatga ttaatcagat tgctaaccag ccatgccacg atggcggaaa taatcaccgg
    32941 gccgagagag gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc
    33001 aagcggggca cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt
    33061 ccattcctgg cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag
    33121 aatcatcgca ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag
    33181 cgatttaacc aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta
    33241 atcggtcggc gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc
    33301 cgccgccagt ccgccgagcg ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc
    33361 tgcattgacc agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc
    33421 atgacgaaac ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag
    33481 acggcggaac atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg
    33541 gcaatgaaac acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg
    33601 ctttaagaga taaaaaaacg tgacattgta acgtattatt gccgaatcgc gcgtaatcgt
    33661 caccatccgg caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc
    33721 aacagttgtg attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag
    33781 aaagggtgtt ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg
    33841 tgccatgcca gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc
    33901 atcaacagca gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg
    33961 cctttctcgc gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg
    34021 cgatccagcc agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa
    34081 cgctgccagg cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc
    34141 gctaatgacg cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca
    34201 accagaggtg cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc
    34261 gccgcgacag aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt
    34321 gcgggttgcc ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg
    34381 ccataaaaga gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca
    34441 agtccggcaa cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct
    34501 gcgcataaca ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg
    34561 gcttgttgca cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg
    34621 gcacaagcga tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg
    34681 cgggctgccc gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag
    34741 aaagccagcg tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag
    34801 gtcatcatcc agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc
    34861 aacgcttgcg gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa
    34921 aatatggaac cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag
    34981 ttaaacgctc gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc
    35041 aacagccaga ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg
    35101 gcgaaccagc caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta
    35161 aagataacca gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc
    35221 cagataacca gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt
    35281 gggttttgtg aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg
    35341 gcgcagctct tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat
    35401 gggttgtttc ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt
    35461 ttcagcattg tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat
    35521 acaaacatct tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg
    35581 gcagactttt gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc
    35641 gaacacgttc tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata
    35701 aatacggcgg aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca
    35761 ggttttacag ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg
    35821 ctactcctta aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcca
    35881 gcggtgaagg gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac
    35941 caaacatatc cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac
    36001 gcggagtcac ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat
    36061 cgccatgacg gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc
    36121 aggcctgctg taacacatca atattgccgt agctggagtg ggtacgcgct ttggtttggc
    36181 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
    36241 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
    36301 tgttggcatc aggtcaggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
    36361 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
    36421 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
    36481 gacatccagt ttttcttttc cacgaacacc atcacgggtc gcggtcattt tcgtaccatg
    36541 gtcgatggca tctgtccatg taaaaacgga aatctgcgtt ttaacaggat tctcgagcat
    36601 cgggaaccat tctaccccca gatcccagct accttcgcgt acacctgagt tgccgccgtt
    36661 tatgccgacg ttaccggtaa gaacggaaag catggcaata gcgcgggacg tttgttcgcc
    36721 gttggagtgt cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat
    36781 ctctcgtgcc aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc
    36841 cggcgtttta gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt
    36901 acgtggtgcg ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa
    36961 tggctgatcg accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc
    37021 atcggtgcca gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata
    37081 gcgtggatcg atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta
    37141 agtgacacca ccgccgctca tccgcgtttc tgccgggtga tttccgaaca taacgaccag
    37201 tttcgtattg gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat
    37261 tgcggcactg atctgtgcgg tactgtagct gccatagcga ctgagaaaac ccccgcaaga
    37321 gttcatcaga cggtacggga cgttcgagtt ggtgatattt ccgccatcta cgcctgttcc
    37381 gtacaggaca tgtacagcct cattgccgta atctttcagg atccgccgaa gattatcact
    37441 gatggtatcc agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgctcgcc
    37501 gacgcgcttc atgggatatt tcaacctgtc aggatgattc atccgtcggc ggatagagcg
    37561 cccgcgtaaa catgcccgaa cctgatgatt accgtaaacg tcgtcacctg tcgtatcaga
    37621 ctccacccag tacacggtgt catctttcac atgcaaacgt agcagacagc ggctcccgca
    37681 gttaacggtg caggaactcc agaccgtttt ctcttctacc ggagcctctg ccgcccggac
    37741 catttgggaa aatggcagag tgaaagcact gcttgccagc gcgagactgc caagtgcgga
    37801 ggttttcatc agacttctat ggctgatttc agccttcatg agcgcctctg tggtatggat
    37861 tttcatcatt actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt
    37921 agtgggttat attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag
    37981 tggtgaaggg cgcagagtgg ttagcgtatt cacacatccg ccatggtcga ttttatcgcc
    38041 agacatattg ggctcgtgcc aggctccctg gcccatagcg ctaactccag ggagaatgcg
    38101 tggtgttact ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc
    38161 gccgttggca atcccacgtt tctgtgcatc tatagggttg atccacacct cctgacggca
    38221 ggctgccttc aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa
    38281 accaaacagt tgcagtggga aggttctacg ttcaggggag tcccagcctt caaaggttga
    38341 ggcataaact ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat
    38401 ttccgccagc ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc
    38461 ctcggggtcg tcacgaaatg ctttgtaggc gacaaaatgg ccattgggat ctttacgctt
    38521 atagataccc atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat
    38581 tttggcgtac agatgttgta accattgttc ctgcgtgcga ccttctgtga acttttgata
    38641 gacgtcaggt ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttacgttc
    38701 gaatttttcg ctggtgactg gctggaggaa aatgagatat cccatgttac cggcgtagtc
    38761 gttaggaata atatcttcct gctcaacggt catcaggtct gccagcagaa tgtcggcata
    38821 ttttgccgat gaggtcataa agttttcgat gaccacaatc atttcgcatt tcgattcgtc
    38881 ctgcagaatt tcatgcgttt tgttgatgtc agaatgctga ttaacgaggg tatttcccgc
    38941 gtagttccag atggactcta ctgtcagacg gatggacgga cttgaagtag ttgactcaac
    39001 gctacttaag gtcctgatat gactgacatt aaccatgacg atactgcata tccatccagt
    39061 tctttctctg ggtctcccta tctgtatcag caagtctaca cctcaagtcc tgacacaagg
    39121 ctgacacaaa tcaaggacct tgctacgcca cagctagtcc ggagaaccaa cgggcgttac
    39181 actgtccgct tcagacttaa aggagctacc agcccgtttc tatcagtctc aacccgcagc
    39241 acagaccgga ggaccgctac aatgcgtcag agagagctcg cagccacagc taaggcattc
    39301 ctactggaca atcctgaagt cacactacag gagcttacag cgcacatacg gtccatggct
    39361 gagcagttcc tgactgatgc cagtgatgac tactggaatg gcgtagaggt ggcgacactg
    39421 gttgacgaga agtccaacct cagggaactt gcagcgacaa gagcgttgag tgtggaccag
    39481 cagaggggca ttaagttagc gctggaagtc ctgaccgcag cacagcagag agtcgacact
    39541 ggtgatacct ctggtctgat taagctgatt gatggtggcg taagccagct tactgatgat
    39601 aataaccata ctgattactc aactataggc gactctacgt cgattctgaa gaatgagcag
    39661 ggggatagac cagcagtttt cacacaggaa cgtcagttac tcactccaga gtcacgagaa
    39721 tcatctgtac tttttcagga attggtgaca tcactgctgg ctgagaaggt ccagacactg
    39781 aaacaatcca gctttaagga cttgtcagcg tccct
//
LOCUS       DAEEGA010000030        38774 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEEGA010000030 DAEEGA010000000
VERSION     DAEEGA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38774
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.030"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="KLH46_001685"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="KLH46_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBE0378400.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..570
                     /gene="hisL"
                     /locus_tag="KLH46_001686"
     CDS             529..570
                     /gene="hisL"
                     /locus_tag="KLH46_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBE0378401.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    546..669
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            716..1615
                     /gene="hisG"
                     /locus_tag="KLH46_001687"
     CDS             716..1615
                     /gene="hisG"
                     /locus_tag="KLH46_001687"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBE0378402.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1621..2925
                     /gene="hisD"
                     /locus_tag="KLH46_001688"
     CDS             1621..2925
                     /gene="hisD"
                     /locus_tag="KLH46_001688"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBE0378403.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2922..3992
                     /gene="hisC"
                     /locus_tag="KLH46_001689"
     CDS             2922..3992
                     /gene="hisC"
                     /locus_tag="KLH46_001689"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBE0378404.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3992..5059
                     /gene="hisB"
                     /locus_tag="KLH46_001690"
     CDS             3992..5059
                     /gene="hisB"
                     /locus_tag="KLH46_001690"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBE0378405.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5059..5649
                     /gene="hisH"
                     /locus_tag="KLH46_001691"
     CDS             5059..5649
                     /gene="hisH"
                     /locus_tag="KLH46_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBE0378406.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5649..6386
                     /gene="hisA"
                     /locus_tag="KLH46_001692"
     CDS             5649..6386
                     /gene="hisA"
                     /locus_tag="KLH46_001692"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBE0378407.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6368..7144
                     /gene="hisF"
                     /locus_tag="KLH46_001693"
     CDS             6368..7144
                     /gene="hisF"
                     /locus_tag="KLH46_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBE0378408.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7138..7749
                     /locus_tag="KLH46_001694"
     CDS             7138..7749
                     /locus_tag="KLH46_001694"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBE0378409.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="KLH46_001695"
     CDS             complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="KLH46_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBE0378410.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="KLH46_001696"
                     /pseudo
     CDS             complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="KLH46_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="KLH46_001697"
     CDS             complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="KLH46_001697"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBE0378411.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11943..12302)
                     /locus_tag="KLH46_001698"
     CDS             complement(11943..12302)
                     /locus_tag="KLH46_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378412.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12299..12805)
                     /locus_tag="KLH46_001699"
     CDS             complement(12299..12805)
                     /locus_tag="KLH46_001699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378413.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12951..13514)
                     /locus_tag="KLH46_001700"
     CDS             complement(12951..13514)
                     /locus_tag="KLH46_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378414.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13721..14659)
                     /locus_tag="KLH46_001701"
                     /pseudo
     CDS             complement(13721..14659)
                     /locus_tag="KLH46_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14722..15096)
                     /locus_tag="KLH46_001702"
     CDS             complement(14722..15096)
                     /locus_tag="KLH46_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HBE0378415.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="KLH46_001703"
     CDS             complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="KLH46_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HBE0378416.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="KLH46_001704"
     CDS             complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="KLH46_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HBE0378417.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17166..18056)
                     /locus_tag="KLH46_001705"
     CDS             complement(17166..18056)
                     /locus_tag="KLH46_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HBE0378418.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="KLH46_001706"
     CDS             complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="KLH46_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HBE0378419.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYKINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="KLH46_001707"
     CDS             complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="KLH46_001707"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBE0378420.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="KLH46_001708"
     CDS             complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="KLH46_001708"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBE0378421.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="KLH46_001709"
     CDS             complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="KLH46_001709"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBE0378422.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="KLH46_001710"
     CDS             complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="KLH46_001710"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBE0378423.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23202..24095)
                     /gene="galF"
                     /locus_tag="KLH46_001711"
     CDS             complement(23202..24095)
                     /gene="galF"
                     /locus_tag="KLH46_001711"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HBE0378424.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="KLH46_001712"
     CDS             complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="KLH46_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HBE0378425.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRCSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="KLH46_001713"
     CDS             complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="KLH46_001713"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HBE0378426.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="KLH46_001714"
     CDS             complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="KLH46_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HBE0378427.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="KLH46_001715"
     CDS             complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="KLH46_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HBE0378428.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="KLH46_001716"
     CDS             complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="KLH46_001716"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBE0378429.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVVGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="KLH46_001717"
                     /gene_synonym="manB"
     CDS             complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="KLH46_001717"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBE0378430.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="KLH46_001718"
     CDS             complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="KLH46_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBE0378431.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="KLH46_001719"
     CDS             complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="KLH46_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBE0378432.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQSGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35503..35982)
                     /gene="gmm"
                     /locus_tag="KLH46_001720"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35503..35982)
                     /gene="gmm"
                     /locus_tag="KLH46_001720"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBE0378433.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35982..36940)
                     /gene="fcl"
                     /locus_tag="KLH46_001721"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(35982..36940)
                     /gene="fcl"
                     /locus_tag="KLH46_001721"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(36943..38064)
                     /gene="gmd"
                     /locus_tag="KLH46_001722"
     CDS             complement(36943..38064)
                     /gene="gmd"
                     /locus_tag="KLH46_001722"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBE0378434.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38090..38638)
                     /gene="wcaF"
                     /locus_tag="KLH46_001723"
     CDS             complement(38090..38638)
                     /gene="wcaF"
                     /locus_tag="KLH46_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBE0378435.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38654..>38774)
                     /locus_tag="KLH46_001724"
     CDS             complement(38654..>38774)
                     /locus_tag="KLH46_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBE0378436.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10357 a         9707 c         8723 g         9987 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgaat tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcctgactag tctttcaggc gatgtgtgct ggaagacatt
      601 cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac cttccggggg
      661 cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata agaaaatgac
      721 agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg atgactcacg
      781 cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc tgatcgcgat
      841 ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc cctgtctggt
      901 aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag aagagctgct
      961 taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc tggatttcgg
     1021 cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc cgctctcctt
     1081 aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc tcgaccagaa
     1141 aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc cgcgtgccgg
     1201 gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag ctaacggcct
     1261 gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg atggcgaaat
     1321 ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg gtgtgatcca
     1381 ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctgg atgaagtcat
     1441 cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg atcaacagcg
     1501 cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg aaaaactgaa
     1561 agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg agtgatcgcc
     1621 atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca acgccagctg
     1681 ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa cgatattctc
     1741 gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa gtttgataaa
     1801 accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag cgaacgcctg
     1861 agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac cttccacact
     1921 gcgcaaaaac tgccgccggt agatgtagaa acgcagctag gcgtgcgttg ccagcaggtc
     1981 acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc gctcttctca
     2041 acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt ggtgctgtgc
     2101 tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga tgtgcaggac
     2161 gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac ggaatctgtg
     2221 ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc aaaacgccag
     2281 gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc ggaagtgctg
     2341 gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct ttctcaggct
     2401 gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat ggcgcgtcgc
     2461 gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac cacccgccag
     2521 gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt ggagatctcc
     2581 aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga actggtcgat
     2641 ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc ggcaggcgac
     2701 tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac ctgttccagc
     2761 ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa agaaggtttc
     2821 tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac cgcccacaaa
     2881 aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc gtgactatta
     2941 ctgatttagc gcgtgaaaac gtccgcaacc tgacaccgta tcagtcggcg cgtcgtctgg
     3001 gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg gagtttaagc
     3061 ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg attgaaaatt
     3121 acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggc gcggacgaag
     3181 gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc ctctactgcc
     3241 cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa tgccgcacag
     3301 tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag ctggacggcg
     3361 taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc aatccgcagg
     3421 attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc gatgaagctt
     3481 atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat ccgcacctgg
     3541 cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc ggatttacgc
     3601 tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac ccgctctcga
     3661 cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc gccatgagcg
     3721 aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg aaagagatcc
     3781 cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc tttaaagcct
     3841 ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat cagaataaac
     3901 aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa agccagcgcg
     3961 tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt ttatcgatcg
     4021 cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt ttgacaaact
     4081 cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagcgg gctacaagct
     4141 ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag cggattttga
     4201 tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt ttgatgaagt
     4261 gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga aagtaaaact
     4321 ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg taattggcga
     4381 tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac gctacgaccg
     4441 cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc gttacgccca
     4501 tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg atcgcgaagg
     4561 tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc agatcgccac
     4621 ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg acgatcacca
     4681 caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc tcggcgacaa
     4741 acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg cccgctgcgc
     4801 actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct accagcgtgt
     4861 gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt acaccatggg
     4921 cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag aaagcctgtt
     4981 caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca ccctgccctc
     5041 gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc caacctgaac
     5101 tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg tgacccggac
     5161 attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca agcagcgatg
     5221 gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca accggtgctg
     5281 ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa tggcgtcgac
     5341 ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct gccactgcca
     5401 catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca ggggattgaa
     5461 gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc gtggaccatc
     5521 gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa cttctacggc
     5581 gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa aaacttcctg
     5641 gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg cgtctccatc
     5701 agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc ttacaggatt
     5761 acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca aaagatccgg
     5821 ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc ccggtgcagg
     5881 ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct ggcgttgcgc
     5941 gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc tggtttgaac
     6001 gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag caaggcaaca
     6061 agcaagtggc agtcagcggc tggcaagaga actcgggcgt ttcactggaa caactggtgg
     6121 aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg cgcgacggca
     6181 cgctggcggg atctaacgtc tctttatatg aagaagtgtg tgccagatat ccgcaagtgg
     6241 cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta cgtggcactg
     6301 gcgtgcgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc gtgaaggagg
     6361 ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg gtcaggtggt
     6421 gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac tggcaaaacg
     6481 ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt ccagcgatgg
     6541 ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca ttccgttttg
     6601 tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct ttggcgcgga
     6661 taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc gcctagccga
     6721 tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg ccgaaaccgg
     6781 taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca ctcaatggga
     6841 aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg tcctcaacat
     6901 gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa aagtgcgtga
     6961 agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac acttcctgga
     7021 agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc acaaacaaat
     7081 aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga tcaggatatg
     7141 ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct gatgccggtg
     7201 attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa cccggaagcc
     7261 ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa acagcgactg
     7321 tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc cccggactgc
     7381 gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca caaaggcacc
     7441 agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact ggaacaactg
     7501 ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact gtatgccagc
     7561 ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc actggcagca
     7621 acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta tcacctgctg
     7681 gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct gcataaacgg
     7741 catcagtgag ttacgggctg agcaggtgtg atgttgttgt cacacccgga gccattgccc
     7801 gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg ataagatgcg
     7861 ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc gtttctctta
     7921 aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc caacacaatc
     7981 cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc ctttttcgga
     8041 ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg aatatcaaga
     8101 tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata gtagtttggt
     8161 gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc ttcgctccct
     8221 agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg ctttgtcacc
     8281 tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag atctttctgc
     8341 tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg tcccagagca
     8401 atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac ttgctgaatg
     8461 tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga aacagtcaat
     8521 ggtaattgct ggttcttaac agaaggttcg atggtaagtt tttctggctc ttcctgatta
     8581 tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa cgtctcctgc
     8641 aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt gttatagcca
     8701 gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt ctccttcgct
     8761 actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat aattgtcatc
     8821 ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg gtcatggttt
     8881 tgcccagaaa cattattatt ttctactctc atcattaccc taactgacgg aaatttccaa
     8941 gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata aaaataatga
     9001 attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa caagatgtta
     9061 gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa gctcttctgc
     9121 catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat cacgttccag
     9181 gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca cttcaacgcc
     9241 tttcgccttg atacgcttca taatcccctg aatagaagac gcacggaagt tatctgaccc
     9301 gctcttcata atcagacgat aaatacccac cacttgcggt ttgcatcgac aattgccgag
     9361 atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc tttcggcaga
     9421 caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaatacg cggatcgaga
     9481 caaacgcctt cgataatctg gcgagtattc agacctaaac tttctgcata actatccagt
     9541 tcattaaagt atgctacgcg catcgccaga taggtattag cgaaaagttt aatcgcttct
     9601 gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgcgcc ttcctgtaac
     9661 aacgcagcaa aacgttctgc gcgttctgaa cgctcaccga tgacaatacg tgacggatgg
     9721 agattatcgt aaagggcttt accttcgcgg aggaattccg gagagaaaat aatattttca
     9781 gtacgatatt tcttatgcat cgctgcggta aaaccaacgg gaaccgttga tttgatgacc
     9841 ataaccgcat aaggatttat ctcaactacg tctttaatta ctgattctac actggatgta
     9901 ttgaaataat tagttttagg atcatagtcg gttggagtgg cgatgatgac ataatcagca
     9961 tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc tgattgcaaa
    10021 aactgctgaa tttccttatc aacaatagga gatatccgat cattcagcat agcaacgcgt
    10081 gacggtaaaa tatctaatgc cacaacctca tgattttgtg cgattagaag cccgtttgac
    10141 aagcctacat agccagtacc ggaaatggtg attttcattt catgctctca gaattaactt
    10201 aactgtgaat catgatgttt ttagcatcgt gataagatgc taaaagtttt aacgctacgt
    10261 tgtcagatgg tcaacgcaac gaaaaacaat tcagattttc tttataagaa tagctgatgt
    10321 ttattaagtt aatattaagc agtgaaattt agtctataag atatttggca aaaaaaagcc
    10381 cggtgcaata tacgccgggc ctcaatttta ttgttggtta aatcagatta atccagccat
    10441 tcagtatgga acacaccttc tttatcaatg cgcttataag tatgcgcccc gaaatagtca
    10501 cgctgtgcct ggatcaggtt cgcaggcaga accgcggcac ggtagctatc gtaataggca
    10561 accgcagcgg cgaaggtcgg aaccgggata ccgttctgta ctgcataagc gacgacatcg
    10621 cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt acggggccag cagcaggtta
    10681 gcgatctgcg gattttcggc ataagcatcg gtgattttct gcaggaactg cgcacggatg
    10741 atgcagccag cacggaaaat cttcgcgatt tcgccgtagt tcagatccca attgtattct
    10801 tcagacgcag cacgcagctg agagaagccc tgagcgtaag aaacgatttt gcccagatac
    10861 agcgcactgc gaactttttc gatgaactca gccttgtcgc ctgctgactg agcttgcgga
    10921 ccagagagaa ctttagatgc ggcaacgcgc tgatctttca gagaagagat ataacgtgca
    10981 aacacagact cggtaatcag cgacagcggt tcgccgagat ccagcgcgct ctggctggtc
    11041 catttaccgg tgcctttgtt tgctgcttca tccagaatca catcaaccag gtagttaccg
    11101 tcttcatctt ttttggtgaa gatatcttta gtgatgtcga tcaggtagct gctaagttca
    11161 ccgttattcc actcggtgaa agtctgggcc agttcttcat tagatagatt caggccacct
    11221 ttgagcagag aatacgcttc agcaatgagc tgcatatctc cgtattcaat accattgtga
    11281 accatcttca cataatggcc tgcgccgtcg gcaccaatat aagtaacgca tggctcgccg
    11341 tcttctgcca cggcagcgat tttagtcagg ataggcgcaa ccagctcata agcctctttc
    11401 tgcccgccag gcataataga aggacctttc agcgcgccct cttcaccgcc ggaaacaccg
    11461 gtaccgatga agttaaagcc ctctgctgaa agctcacgat tacgacgaat ggtgtcctgg
    11521 aagaaggtat taccaccatc aatgatgatg tcacctttat cgaggtatgg tttcagggaa
    11581 tcaatcgcag catccgtgcc tgcacctgct ttcaccatta acaggatgcg acgaggcgtt
    11641 tccagagatt caacaaactc tttcaccgta tagtaaggag ccagtttctt gcctggattt
    11701 tcagcaatca cttcttccgt cttttcgcgg gagcggttga aaatagagac ggtataacca
    11761 cgactttcga tgttgagcgc aaggttgcgc cccatcactg ccataccgac tacgccgatc
    11821 tgttgctttg acattgttta ctcctgtctt ctatattatt aaccgtctat taaccgttat
    11881 attattaacc gtcgtgattt tcatcttcta atcaaggaat acatatggtt acagcatgct
    11941 tcttacttta tagcgataag tatgccattg ggtgtatcca taagaacttt gaattttgga
    12001 tacttttctg gattaaaatt agcatagctt cgtttagata taacaataaa cgaactatta
    12061 aaagatttca tcttctctat taaatcatcc tgtgggatta cctcacactc gggattacta
    12121 atagtccaat cactatatct tgctaatcca aagacgtcac caattgccat ccctttatag
    12181 aagtaaatac catcctccaa gcctaattgg ataagattat ctccatataa ctcactatat
    12241 tcacttgctt tcttcatcag ttcatagcta tagcgacttt ctaagcgtga attaaaattc
    12301 aagtattcag aataaaaata tttgaattca aaatacaacg atcgccccga aaaaaaataa
    12361 tacacacaag gataaaatat cgtcccaact tgtaatttgt aatgttgata tcttttatta
    12421 aacttgcaat tgagatcgca acctgcaata ctgacaatgg gtaaatgggt gataaatacc
    12481 tgtcaccttg cgtaacagaa aaccaaaata agacatatgt taaaacaatc gagaaaaata
    12541 cccaaagtat tttattattc ctaagtgtaa agacagacag tatagcataa aaaacaacat
    12601 cactgccaac tttattaatt gcttgttgca aatctaatgt ggcaggactt ataccatgtc
    12661 gagcttgttc tgctgtttga acttgtagat caatttcatt ccataggtag tgaccaaata
    12721 tatttcctcc agcaggacta aaaggatcac cagagatcca aaaactacgg acataccaat
    12781 aaatgccacc aagaattgtt gccaagctaa aaataagaaa tgtctgtatg tttctctttt
    12841 tcgctatcaa tataacaaat atgatggctc catatatggc accaaatagt tttacgccac
    12901 aagcaattcc aataaaaaaa ccacataaaa aaattaattc attttgtgtt ttattgtttt
    12961 ctgagcgaat atatataaga atactggctg cagcaaaaca aagatagcgt taaatcaata
    13021 tatgcataac caagatgatc tttaagtgga ttgatagtaa atagtaggat tgtagcaata
    13081 atgcctggat aataagtttt cgtaatttcc catgaacagc caacaagtcc acaggctatt
    13141 acaaaaaaac agatatttgc taaaaactga gctaacctta catctcctaa ctgtaaacct
    13201 aaagccatta aaagatctgc attttgcggg aacagaggaa aacgcaaata ttgttcaaca
    13261 acaattgcat ggtgctcaac taatgaacga gccaaaggta agtgatacat agtatcatcc
    13321 caatcaccgg gagctcgcag ggacgataac aatgtaacca caaaaatcaa aacaagaaat
    13381 aaaataacaa acagcgtact gaactcaata ttatttattg ccagccatct aaattgctga
    13441 cgtttaataa tgcttaaaac ccaacacacc gtaaaaatta aaaagatagc aatcttatta
    13501 aattgcccga ccatgcccag aaagtaaatt atcaaaatat ttaaagacat ccctatgcat
    13561 aggcaataaa aaataaagaa aatttgttaa gtcctgccac cccctcctta aaaaaagatg
    13621 acaatacaat attaccaatg acaaaaaatg tcattccaaa agcaaaacaa tacacaacat
    13681 acaaatacaa cggcaattca atcatagtaa aatactctat ttacattatc agtttatagt
    13741 ttttttcaga aagataaaat aatgccttca tttcacgtct agttgttgta tttgaatgca
    13801 aaataatgcc acatacaaat gccattattg acgataacat gatggaggca gttaaaaagg
    13861 cagtcggaaa tcttctcacc aaacctgtat caatataatc aacaatgatg ggcaatccta
    13921 aaattataga caaagcagca agtaaaacgc cgataatact aaaaaagtat agtggccgtt
    13981 caagcgagta aagtttaatt attgttttca gaatcttgaa cccatcactc caagttgata
    14041 gtttgctcac actcccttca gatcgctcgc catattttgt attaacctca ccatacttca
    14101 tacgtaattc taaagcatga attgtaagct cagtttcgat ttcaaatccc cttgatagac
    14161 atggaaaaga tttaacatag cgtctggaaa atacacgata ccccgacaac atatcagtaa
    14221 aattcccttt aaagatagtc tgaacagtac cagtcaacag tttatttcct cagcgatgcc
    14281 cttttctgta tgtattttga tctccattct cttgacgaca tcctacaacc atatccaatt
    14341 gattttttat gagatggtta atcatttctg gagcagaact tgcatcataa gtgtcatctc
    14401 catcaaccat tagataaata tccgcatcta catctgagaa catacgtctt acgacattcc
    14461 ccttgccttt cagaggaaca gagtgaactg ttgctccagc atcctctgcc acttgtgctg
    14521 tactgtcagt cgaattatta tcaaatacat atatagaagc agttggaata ttcgttttga
    14581 aatcatttat gacttttgct attgccttct gctcattata acaaggaata attacagcga
    14641 tattaaaaga gtgatacata agcccctcat ataaactatt tagctaatgt gcaagtaatt
    14701 gtgtttttaa caaatcgttt actaacgaaa aatgaaatat tttgatgcgc aatatcctaa
    14761 aaccaaacta aaaagagaaa aggtgaacaa tgtaaatatt ggagataaag ctaagagatc
    14821 aaataaaacg ccgaataaat aactcatcgc ccccataaaa aatataaata taaaatatct
    14881 agccttggtt gcagatactt taaatgagca tcttgcattt gcaaaaaaag aaaaggttgc
    14941 tgcacatata aaaccaacaa tgttacttcg gccttgacca aaagctaaat tattataaca
    15001 cgcataaaat accccccaat gtaaagcagt attgacaatt ccaattaaaa aataactaaa
    15061 aaaagaactg gtcaataact tccctatttt taacatcctt tattttgctc cagaagtgag
    15121 gttattacta atttggatat tttctataga aaatacccat aaaactacaa cgcctataat
    15181 aaaaattccc atgggtgtaa aaagaaatgg attagttata gaaacggtaa tactgaaaaa
    15241 aagtattaac gacatcatta atgatgtttt tgtactattt aaaaaatagc ctttcaaaaa
    15301 gataagcaac ataggaacta atgatataaa tacaccaata acgcctgttt tctgaagtat
    15361 ctcaagaggt actatttcaa tccttaatcg atctaatata aatgaaccaa atcctcttcc
    15421 aaacaagaac gtcgctaaat caacattttt ataataaaaa tataaatcat taaatctaac
    15481 agagtcagaa tcttcactgc gtgatccgac catgtaaagc ataattataa atagaacgct
    15541 gcccaatatt attataatag tatttaaata tagcttatgt aatttaaaac tggctaataa
    15601 cagtgataat gttgtaaaaa gtaatacacc tcttgattct gtcaataata tcgcaagcac
    15661 tattatgaga cattttaaat attttttgtc gacaaaactg ataatcgcac cgacaccaaa
    15721 aaagtagaag gatttggaaa aaaaagcccc atcgggacga aaaaagaact cgctagtcaa
    15781 tgataagtgt tcataaatta aattaaaatt taacaacccg aaattcaaca aaattatgta
    15841 tgaaatatat aacagtgatc catataatgc aaatgcatta actattttaa taaaaatctc
    15901 acatgaaaat acttttaaag tatatcttag tgtaaataga tatataagtg acagcataaa
    15961 aaggtatggt tgcacatcaa acagtatatc atttatttta ttaccagcta ataagccgac
    16021 aaagaagcca taaaaaggaa ataaaagcaa ccatataaaa agactgacac atttttgggt
    16081 gattgaaaaa ttgaatgtaa ctaaattaaa aattaaaagt ataaaactaa atacaaataa
    16141 taaatatcgc aaagggaata ttcccaatgg ctccagtaat cttccaccac caccaataat
    16201 cagttcaata cataaaaatg ttataaaaat tttatttata ttattcatat tcgtaaggtg
    16261 atgtttttta aaatatgttt aaaatcaaca ttaataaaat ataaatttta tcagcctttt
    16321 tccgccttaa cttccaaaaa cttaaagcca agatcaaatt gcctatataa cctttaccca
    16381 attgattatt cacaaagcta tcatcagcta aggcattagc tattttagtt acttcttttc
    16441 gataccactt gtttctcacc aaaccaagcc gtttgtattt tgctttaaaa ctaatgtttg
    16501 ctcccacttg gttgttttca tgctgtctgt acagcattgt tggttttcta tcaatatacc
    16561 acgaataatt tctcgtccgc gcaaatgaat ataaaaacca atcatgcaga cagatatctt
    16621 gtgcagcatt tttattattt atgatgaact tcttaaattc aataagcgtt tcttttttta
    16681 taacatatgt acaccccgga cctgctgctt caaaaaaata atcgaattga gtttgcgact
    16741 gagccttatc aactaaattt tttcgccctg atggataata tgcaattaca ttgctggaat
    16801 aacaattcgc attattaaat accaagcagt caattgcatt ttttatttta tctttatacc
    16861 aaatatcgtc ctgatcacta aaacagatat aatcatagtt ttcaagatcg acatctttaa
    16921 ttaatctata aaaattttta cctgcccccc caaacttatt gacgcttgga agtatattta
    16981 taattgaggg atatgataat tgaaattctt cacaaatatt taatgtatta tcagttgata
    17041 aatcatctga tataaaaatt gagatgtcta catctttttg ttcaataata ctttttaatt
    17101 gttcctcaat ccagcattcg ccattatacg tagccataag tacagctact cttttttgat
    17161 cagaactatg cattataacg accgccccca gtaattcctc ttattccatc cagcgtagcc
    17221 aaaatatgtt ttttcatata ttcaatttta tttttttgag ttgcgataat taaagtcgta
    17281 tgaattaaag aaaaaacaac ttccctaata gagagtaaaa gaggcacatg attttttcta
    17341 agcaaaagaa atgcatttct tactcgataa taacgacgta ctggcgagtg aattggtatg
    17401 ttaatcccac agatttttac tgacttatcc ccaatagcat gaaccatttc tatatcagga
    17461 atgatatgaa ctaatatgcc atagtttaag catctaagac accattctgt atcgacataa
    17521 tcaataaaaa gttcctcatc catcattcca acaatctcaa aaacttcttt tgaaaccatg
    17581 gttccggatg agattgttac tgaactttta aacggtgtct gtccttcagt gacatgaatt
    17641 ttctctcgaa gaccattttt tttgatatta caaataggat aataatgtga cttatctcta
    17701 tcaataaaaa ctgggccagc tatttttgca ttttcattat ccatatagca ggaaagtttt
    17761 ttgataaact cattgcgaat acggctatct tgatcaaaaa aaattatctt ctctgcccct
    17821 ttttcaagag aatatcgaat cccaatattt tgtgcttttg ctataccttc attcctacca
    17881 agacaaatta aatgaacttt ttcaatgtct gctaatttat ctttcaaggt gaaattagtc
    17941 ctattatcga ctactacaac gtaacaattt tgctccttta gttcagtgat cagtgcattc
    18001 agatttttaa gttcgggatt atatgtcaca atgacagcgt aaatattact attcattatc
    18061 caagtgactc agtaattggt taattttagt tttcatatac agaacacatt ctagtataaa
    18121 gccattgtaa taacagagca tctaaaatta cacgtatgct ccaggatatt gcggcaccca
    18181 gtaacccata tttttcgacc ataaaataca aaagggctaa ataaggaacc aattcagcac
    18241 aatgaatcaa agcagtaatt ttagattttc ctaatgcctg gattgcagag aatggtatca
    18301 ttgccagcgc attgaagaaa aaaccgatca ataacacatt taatatatta aaggaattaa
    18361 ttttataatt ctcaccaaac catattttca atacaagccc tgaatataac aagcctatga
    18421 ttatcaccgg caaacagatt agaaacatta aaagcaatga tttatttaca ttacgtttaa
    18481 attctttaaa gtctttgatg ttacttaacc ttggaaacac tgccctcccg attgctgcag
    18541 gtattattcc taattttaga ataacctccg ctggtgcaga ataaaatgca actttatctg
    18601 cccccataat atttgacact ataaatcgat caaaatacac catgaccgga cttatgatat
    18661 tacttactgt catccaaccg ccaaagaaaa agagtcgttt aaaggtttta taacaaaaat
    18721 gaacccccga aattttaata tcattcctga cataataggc agagactaaa atcgaaataa
    18781 ctcttgcaaa tattaaacca gcaaccgctg ccgacaatgt agcactataa aaaacaaata
    18841 tggccggaat tcccgcgatg caagagcttg atatagattt ttgaatattt acaatgccaa
    18901 atttttcatc cccctcaaga atggctgacc ataattgatt tagaataaat aatggaatgc
    18961 aaatagccaa cagtttaaat gctagttgta tatcactatg ttcaacacca gaaattttta
    19021 gataattaac aattccatca gagaaaatca ataataaaaa agcaccaaag catgaaaata
    19081 gcaccaaaaa agatgtactt gttgaaatta ccttggttct ttcatgatga ttatcacgat
    19141 gaattgcaat ttctctaata acagagcgag tcaggcctac atcaaaaatt ccagcatacc
    19201 caacaagtgc aattgcaatt gtataaatac cgaaaccttc aggccccaaa cctcgagcga
    19261 gaaatcctag cgaggggata gcaattaaag ttggtattgc atagccaaaa aggttccaga
    19321 cactattttt tattatgctc attattacat ttctttatca tataaaataa tcagcatcta
    19381 caaatttttg accattaata tctttatcag aaagcaatgg attttgaacc ggccattcta
    19441 tatttatact tttgtcgctc cataaaatcc cacgctcttg catttttgaa tagaaattat
    19501 ttgttttgta gacaaattct gcttcatcac taagaacgca aaacccatga gcgaatcctt
    19561 ccggtatcca taattgacgc ttattctccg ccgacaaatt tacaccaacc cacttgccaa
    19621 atgttggtga tgatttccga atatcaacag ctacgtcaaa aacttcaccg acaacacagc
    19681 gtaccagttt tccttgagca taaggttcca gctgatagtg cagtccgcgt aacacaccct
    19741 tagttgattt agaatggtta tcctgaacaa actcaacctt tcgtcctaca gcctcttcga
    19801 aaactttctg attgaagctt tccataaaaa agccccgctc atcaccaaat acttttggtt
    19861 caaaaattag cacatcagga atttcagttt taattacgtt cattttatta ataaccttta
    19921 atcattttca gcagatactg accataagca tttttcttca gcggctcggc taataccttc
    19981 acttgctcag catcaataaa gcctttacgg taagcaattt cttccgggca ggaaactttc
    20041 agcccctgac gctcttcaat tgttgcaatg aagttgcttg cttcaataag actttgatgt
    20101 gtccccgtgt ccagccatgc ataaccacgc cccatcatag cgacagacaa acgtccttgg
    20161 tccatataaa tacggttaat atcggtaatt tccagttcgc cacgtgcaga tggtttaagg
    20221 tttttcgcca tttctacaac gtcattgtca tagaaataaa gcccagtaac cgcatagtta
    20281 ctttttggtt ccagcggttt ttcttccaga ctaattgccg taccgttatc atcaaattcc
    20341 accacaccgt agcgctcagg atcatttacg tgataggcaa aaaccgttgc gccactctct
    20401 ctgttaacag cggtatccat taatttcggc aggtcgtgac catagaagat attatcacca
    20461 agtaccagcg cacaatcatc accacctata aattcttcac caatgataaa tgcttgcgcc
    20521 agaccatccg gactcggttg tactttatat tgcagattaa gcccccactg gcttccatca
    20581 ccaagcaatt gttgaaaacg tggtgtatcc tgcggcgtac tgataatcag aatatctcga
    20641 atacccgcca acataagcgt tgaaagcgga taataaatca tcggtttatc ataaatcggt
    20701 agcagttgtt tgctcacagc cattgtcaca ggataaagac gagtgccgga accaccagcc
    20761 agaataatac ctttacgcgt tttcatttca ccattccttt taattcatct cgctctagca
    20821 tcatgagcga gatgcaataa tttgttaaat tgccgtggtc gtaaataatt cgttgagcat
    20881 acgtttcacg cccacctgcc agtcaggcaa gacaagcgca aagttctgct gaaatttttc
    20941 ggtattgagg cgagaattat ggggacgacg tgctggtgtg ggataggccg ttgtcggcac
    21001 ggcattaagt ttgttaagag caaggttgat ccccgctctg cgcgcttctt caaataccag
    21061 agcggcataa tcgtgccagg ttgttgtacc accagcaacc agatggtaca aaccagcaac
    21121 ttctggttta tttgcggcca cgcgaatggc atgagcggtg caatcagcca gtaattcagc
    21181 ccccgttggt gcgccgaatt gatcgtttat tacagccaac tcttcgcgtt cttttgccag
    21241 gcgcaacatc gttttggcaa agttatttcc tttaccagcg tatacccagc tggtacggaa
    21301 aataagatgc tttgcgcaat gttcttgtaa cgctttttcc ccagctagct tggtttcacc
    21361 gtagacattt agcggtgctg ttgcatccgt ttccagccat ggcgtgtcac cgttgcctgg
    21421 gaaaacataa tcagtggagt agtgtataac ccaggctccg acttcattag ctgcttttgc
    21481 aatcgcttcg acgcatgtcg cgttaagtaa ttgcgcgaaa ttcggttctg attccgcttt
    21541 gtctactgca gtgtgagcag cagcattaac aataacatcg gggcgaattt ttttgacggt
    21601 ttcagccaca ccttcggggt tgctgaaatc tccacaataa tcagtggagt gaacatcgag
    21661 cgcaatcaag ttacccagag gagccagagc acgctgcagt tcccaaccca cctgccctgt
    21721 tttgccgaaa agcaggatat tcattactga cggccctcat agttttgttc aatccacgac
    21781 tgataagcac cgcttttcac attctcaacc caatttgtat tagccagata ccattccacc
    21841 gttttacgaa tcccgctctc aaacgtttct tgcggtttcc agcccaattc gcggctaatt
    21901 ttatcggcat caatggcgta acggcggtca tgccccgggc gatcggcaac ataagtaatt
    21961 tgctcacgat aagatttctc tttcggtaca atctcatcca acaaatcaca aatagtgagc
    22021 actacgtcga tgtttttctt ttcgttatgt ccaccaatgt tataagtttc acccgcttta
    22081 ccttcggtta cgacggtata taacgcacga gcatgatcct ctacatacaa ccagtcgcga
    22141 atttgattcc ctttaccata aataggtaat gccttacctt ccagagcatt cagaataacc
    22201 aatggaataa gtttttccgg gaagtgataa ggaccataat tgttcgagca attagtcaca
    22261 atggttggta aaccataagt ccgcttccag gcacgtacca aatgatcgct ggaagcttta
    22321 gacgcagaat acgggctact tggcgcgtaa gctgtcgttt ccgtaaatag cggtaacgct
    22381 tcgttattat ttgcttcatc cggatggggt aagtcaccat aaacttcatc agtagaaata
    22441 tgatgaaagc ggaaactttt tttcttttca tcattcagag cagaccagta attgcgcgcc
    22501 gcttctaaaa gaacataagt ccccacaata ttggtttcaa taaatgccgc agggcccgtt
    22561 attgaacggt caacgtggct ctctgccgcc aggtgcatca ccgcgtctgg ctggtgctgt
    22621 gcgaaaatac gcgacatggc tacagcatcg cagatatctg catgctcaaa agcataacgt
    22681 tcagaatcag aaacatcagc gagtgattcc aggttcccgg cgtatgttaa tttatcgaca
    22741 ttaacaacac tatcttgcgt attatttatt atgtgacgaa caacagcaga accaataaac
    22801 cctgcgccac cagtaacaag tatcttcact tttttattcc atatagctat agagcatgct
    22861 gtgaaataga ctgctccaga tttgattaat agaggtatta atacacgcta ccgcccctgg
    22921 cttaacagct accagtgcac tgcatgcatc tcaacgatgt ggcgagcgtt agccactcgc
    22981 gccaaatccg aaaaattcaa acgttaattg tcttaccaaa cagccgtaga aacaaggaaa
    23041 atcctggaaa aatttgaata atatgagcta tgtaacttct tgtttttata ggctttataa
    23101 aaacggcaac ggcaggaaaa ttcaatgagc aaaacttatg ccacgaatct tcactgccgt
    23161 tataattttc ttatcaaccg ttacatccgg tcagattttc attattcgct taacagcttc
    23221 tcaatacctt tacggaactt cgccccttct ttcaggttgc gtaggccata cttcacaaac
    23281 gcctgcatat agcccatttt tttgccgcag tcgtaactgt cgccggtcat cagcattgca
    23341 tcaacggatt gttttttcgc cagctcggca atagcatcag tcagctgaat acgtccccat
    23401 gcaccaggct gagtacgttc cagttccggc caaatatcgg cagaaagcac atagcgacct
    23461 acggccatga tgtctgagtc cagcgtctgc ggctgatccg gtttttcgat aaattcaaca
    23521 atgcggctga ctttaccctc acggtccagc ggctctttag tctggatgac ggagtattca
    23581 gagaggtcac ccggcatacg ttttgccagc acctggctgc ggcccgtttc gttgaaacgt
    23641 gcaatcatgg cagcaaggtt gtaacgtagc gggtcggcgc tggcatcgtc gatcacaacg
    23701 tctggcagta ccacgacaaa tgggttgtca ccaatggcag gtcgcgcaca caaaatggag
    23761 tggcctaaac ctaaaggttc gccctgacgc acgttcataa tgatcacgcc cggcggacag
    23821 atggactgta cttccgccag cagttgacgc ttcacgcgct gctcaaggag tgattctaac
    23881 tcataagagg tgtcgaagtg gttttcgacc gcgttcttgg acgcgtgagt taccaggagg
    23941 atttctttga tccctgcagc cacaatctcg tcaacaatgt actgaatcat tggcttgtcg
    24001 acgattggta gcatctcttt gggtatcgcc ttagtggcag gcaacatatg catcccgaga
    24061 cccgctacag gaataactgc ttttaaattc gtcattattt catccacctg taaaatggtt
    24121 gctgaattat agcttgttcg atttttttcg ccagcatcaa ttaccctgaa ttgattactg
    24181 aattacttgt gatgttacgc cgcttcgttg tggattgcag tagcattgtt cctaagtatg
    24241 gctccatttt tccaggaatg gtcgcaaatc tactccctcc gttccggcaa tctaaagtta
    24301 atcttttcca cattaacaat atggtgatta atcctgtcga tatcgacgga gctttgccct
    24361 ctttcattca ccgcatgaac atttgcaaga gacagcagtg tttctttttt cgccataaag
    24421 acgccgcgaa cgtctttgcg catgtcgaag ttcatgctca atgctggtcc aactgaggat
    24481 tcctgcatca cattgatatt tcgcataaaa agatgttgcg gcttgttgtg taactccagc
    24541 gaggcacgct tcatctcaat gttagttagc gacacaaagg agacggcatt cccggcggag
    24601 atttggatgc cgcgcaattt ataagcaaga tgggtattat ccagttgaat attattcact
    24661 cggaaatttt gcggtatcga gagatatttg cctttaatta ccccatagcc gattaacatc
    24721 ccggcgctat taatcatttc aatattatca atcacgaaat tgtcacaacc gtaaatagcg
    24781 actgtcgcgt tatcaatgcc cgctttctta ctgaaatccg gcgtgatatt gcgggcattg
    24841 atattacgaa taacaaaatg tttaccattt tcaacatgga tcaactgccg acaatccgat
    24901 cccgtgatat tcgccacgac aaagtttttc actgcctggt cttccgggta gttgttatca
    24961 taagtgcttc ccgcaaggcc tatgccgatg ccccagttga ttttgccgtt ggtacagttg
    25021 atacgctcga tgacatggtc agatatcaaa atatcactgt cgttaattgc cacgttccat
    25081 tcaatggcgt cgccctgtaa gtcgctgaac ttacagttgg taatgttggc accgataatc
    25141 tggttatgaa atccctggcg taagatggcg taattagcgt ggctgacagt gaggttatcg
    25201 atggtcaggt tgcgcatgac ccgtttgttt ttgccgccaa tataaatctg cattaccggg
    25261 ccaaagccgc tcatcgccag ccctttgatg gtgcagtcag aacaacgcac atccagggta
    25321 atgttatgca taccgccgcc ctctccccct gtcacctggc tgccgtcctg taagacaaat
    25381 cgccctctgc cgttgccgcg caggcttcca aggatgtgta acgttttgcc gggagggatg
    25441 aagatgccgg tgttgatatt gtcacaaacg aatccggcag gcacgacgac cgtttgccct
    25501 tcgctgaagg cttgtttaaa tgaggcgatc cagtcatgtg ggttgtagtc gttaatgtta
    25561 acgctttgtc gggcgggaag tgcgcgagcg aaaggggtat ggaggaaggc aagcgccgag
    25621 cttgccgtca ggaaggtgcg tcgggagagt gttttaaatg gcatacgttc tcctctataa
    25681 agcctgcagc aagctggcga gttctcgatt aatcacctgc tggttaaaat cgtgttcgac
    25741 tttttcgcgc gcacgtttga cgaccggagc cagttcgtcg gtatccagtt ggctaaacgc
    25801 cgccagacgt tgtgccagtg cgcgagcatc gttctcaggc agcagccagc cggatttatc
    25861 ggcctccacc agttcaggaa ttccgctatg cagagtagaa accaccggaa taccgaccgc
    25921 catcgcttcc attagcgcca ccggaatgcc ttccatatcg ccatccgcgc ccgttaccga
    25981 tggcaacagg aagacatccg cgtcgtcgag catctccttc acttcatggc tcggtttaaa
    26041 gcccggcatc tctaccacat cttccagttg atattgttcg atgagggtgc gcaggcgtcg
    26101 ttcccacagg ccaatgccga ggatgcgata gcgaaatgcc acgccctgct ctttcaactg
    26161 acggcaggct tcaatcgcca catgcaggcc ttttttctcg gttaagcgtg cgacagatat
    26221 gatttccagc ggcgttgcgg gcgctttcac cggacgcggg ctaaagcgcg tcatgtccac
    26281 gcccatacgc gatacggcga ttttttctct ggggcagccc attttttgca gccttccggc
    26341 ccacagatcg cttatcggta acatcaggtc gccacggcga aacagttgtt gatattcggg
    26401 agtgtagtgg ttgagcactt cccgactgga gatatcaata ccgtggaaga tggtggcaat
    26461 tttgccgcga atgataccca gttcgcgtag ttttgctgcg gttacccccg ctgggccaaa
    26521 atgagcgatg aacacatcgg cgcgaaacgg tgttgcgacc tgaccacaaa tggcagacaa
    26581 aatcaggttc cgcgactcgg caccatagcg tttgaggttg agcgcatgcc aggtattttt
    26641 acgatgaatg ccacgtaagg tctggctggc gcggtggcgc agtttcgcta ctttgcccgc
    26701 aggttcgtcc tgtaaccagc gggttctggc gacaaggttg tatttcgtcc atgccgcgtg
    26761 ggtgttctct gtatcgcctt tttgcaaagc gacaatctcc acttcaaagc ccatatcaat
    26821 aaacgcggta atttgattga ggacgaaggt ttccgacgac agcgggaatt tcaataagaa
    26881 gaagccgacc ttcatttcac ctccccgatg cgctcaagca cagactgcac catctgcatt
    26941 cccgtctgac gttcgcgact gacggcttcg ttaaatcgag cgttcagcac cggaagctgg
    27001 cctaaggtat ccgcaaccat cgcttgcagg ctgccgtcca gtaaatgacg aatatcaatc
    27061 gccatctctg gcagtcccag ctgctgcata atcccggcgg atttatgttc atagttgatg
    27121 gcaatcgccg gagtagcaaa gttcattgag ataatggcgg agtgcaggcg cgtaccgacg
    27181 gtgagttcac aggctccgag aattttgccc atttccagat cgttgagttc atccatcact
    27241 acgtggtaac gggcaggatc gctgatgtgc tggcgcagat tgagcgccac catgcggtcg
    27301 tctttgttat agctgtcgat acccgtacag gtggagagcg caatcacctg atagccttca
    27361 tcgagaatgc gattgaccac cccggcaaag gctttttcat acgcttgttg agtggtgccg
    27421 agacgtttgt cgaacggtgc cagttcgcgc agggtaattg ccactgtttt ctgttgtgcg
    27481 gcaacgtcca gccagtgttg aacggcatag ctggcggtga agtcttcggt gtggtgatcg
    27541 accagccacg cggtatcgac gccatgttcc acttttgcgg tggtgatatt tctgcgtttc
    27601 atcaaatcaa ggctgaccga ttcgcgcagg atcagcgcgt cgcagtgacc aaagacatag
    27661 ttcgccagtt ggttaaattg ctcatcctgg aaggggccga cgctgtgacc aatcataaac
    27721 agcggctttt tcgccataaa tgtgcaaagc gcatgttcaa actgcggcac gccgtagaga
    27781 tcgacaaaaa acgatccgcc gacctgaata atggcgtcgt aacctgacag caggcgcacg
    27841 aagtcggtga atccctgggc gatggcgata ttgcgcagct tgccagtgtc ggtgacgcgt
    27901 gagagcaata cctgatgctg gtagcggcga cggaggactt ttttaatgcg cccgacaacg
    27961 cccgccgcgc tattgtgttg tgtcatctgc aggaacagcg gatcgcccat taccgggcgg
    28021 ttgagcagcc aggaagaact gaccggatag cggctcatca cgtccacttc ggtatgtgga
    28081 ttgaggatgt tgatggcatc aagtaagccg cgcaagatgg cgctgtcgcc acgattgccg
    28141 caagtgtggt tgcccagaat aagtaatttc attttttcct cataaatttg atgccaggtg
    28201 aggctgtgtt tgttgtgttc gttgccgttt tttgcctgat gcgacgctgg cgcgtcttat
    28261 caggcctaca ggtcccgagc acagaaccgt aggacggata aggcgtttta cgccgcatcc
    28321 ggcaaccgtt gtcggaaccg aaaacagcaa ttcaccccgc ccgtaaaagc attttcattt
    28381 tttcgctgcg acaaaactga cgcttcactt ccaccaccag cggatggcgt gacagcacaa
    28441 tcatcaccac aaacgccagc acgcccgcgg ctatttgcac cgccagcagc atccccagcg
    28501 ccagttgccc tttcagcaca atgcccagcg cataactgac caccagcgtc ggcaacgaga
    28561 gataaaacgg cagccacaaa ctcaggatgt actggcgata actggaacca agaaccggtt
    28621 taatcatcac gaagtaactc agaatggtgt tgataatctg caccagcagg aaaccgagcg
    28681 tgacgccgat tgcgcccgcc atctgcccac caataacaat cgccggaata aacagaaatg
    28741 ttttgaatac gttgaattta aagctgatat cgacccgcgc tttcgccatc agcagcgaac
    28801 caatcgggtt acccacggag cgcagcagac ccacaataca cagcaattgc agcaccggaa
    28861 taatgctgtt ccacttttcg ccgaatacca gcggtacgaa gttattcgac accaccatca
    28921 gccccagcag cgccggaaag ttgataatcc ccactatcga cagcagcttg tagaagttaa
    28981 cacgcagctt ttcggtgtcg tcctgaattt tggcaaacgc cggaaacaac acgcgggtga
    29041 tgattgggtt cagcttcatc ggcggtacga cggccacgtt gtacgccagg ttgtatcccc
    29101 ctgccacgcc cgcgccaaga atacgcgcca gcaccagcgt tgaaaggttg gtgttgagat
    29161 agttgatgat gctgtccgcc gtcagccagg caccaaaacg taagttcggt gccacagacg
    29221 ccagcgagaa gtgcagaccg gggcaataaa ttttgcgtcc aaagtagcca aacagcagtg
    29281 ttctcaccgc actattgacc agataaccga ggatcgcggt catcgccagc ggccagaaat
    29341 gggcgctaac taccgtaaaa gtgaagcccg ccagcaccgc gctggtttcg atcatgccga
    29401 ttttgttgaa ctccagctcc ttctgcatca acgcgcggaa ctgttgcccg tgggggatca
    29461 ccacaaacgc cagcgacaat gttttaatca acggtgccaa gtccgggtta ttcagcacgt
    29521 cgccgatgac atcactcaac aaaaacaccg ccacacatac cacaagcccc agcccgacgt
    29581 tcagccagta cagcgtggtg agttcaaggt gactgatttc ttttcgctga ataatcgagt
    29641 tagcgatacc gaagtcagaa agcgtatctg ccagcgcgat aatcaccagc gacacagtca
    29701 gcaggccgaa ctggtggttg tcgataatcc gcgccagcac ggtcatctgc accagcccga
    29761 ggccgatgat gatcaccgtg gcaatagccg accacttcgc gccgctgatg gttttttcac
    29821 gtaagctcat atcaatatgc cgctttgtta acgaagccct tgaataccgt caggaaaacg
    29881 attttgatat cgaaccagac gctccattcg cggatgtact caaggtcgaa ctcgacgcgt
    29941 ttttccattt tctccagcgt gtcggtttcg ccgcgccagc cattaatctg cgcccagccg
    30001 gtaatgcccg gtttcacttt atggcgcagc atgtagcctt caatgagctg gcgatactgt
    30061 tcgttatgcg ctactgcgtg cggacgtgga ccgacaatcg acatcccccc ggtcagcaca
    30121 ttgataaact gcggcaattc gtccagcgag gtgcggcgca gaaagttccc cactttagtg
    30181 acgcgcggat cgttctgcgt cgcctgggtc accactttgt cgttctccat cactttcatg
    30241 gaacggaact tccacacttt gatcggcttg ccatccatac catagcgagt ctggcggaaa
    30301 ataaccggcc ctggtgaact gagtttcacc gccagcgcaa tacagcacag caccggggag
    30361 atcagcagca gaataagcgt cgccagcaca atgtcttccg cacgttttag caggcggtta
    30421 acccccgaaa gcggcgtgtc atacagcggc accaccggaa cgccgttcat ctcttcgaga
    30481 cgtgaatgga gaatgttgaa ggtaaagacg tcggggatca gcagcaccga acaggtggta
    30541 tccgccagtt gatggaccag ttttttcact cgtgcgccgt cgcacatttg catcgcgata
    30601 tagacgttat gaatcttgcc cgcttttgca tcctcgacca gctgttgcag gttacccgcc
    30661 cagtcgttag aaacgccgcc cggtttcggg tcgtggtaaa cgcccaccac ttcaaaccct
    30721 aaccacggct gattacggaa actctccatc agcatttgtc cggcggctaa atcccccact
    30781 acggcgacca tccgcttgtt gtagccatga ttacgcagcc agcccgcccc aatgcgaata
    30841 catgaacggc aaaccaccag tccgatgctg gtcagcccat accacgccag ccagattttc
    30901 agttgcgtgt cgaaatcatt gttgaacgcc accagtccgg cgctgaaaat cacgcttaag
    30961 gtccagtttt gtagcaacag ggcaaattct gtcgctgccc gaacaccgcg ccatgagcga
    31021 taaaaatcgg tgatgccgcc cagcatctgg aacaccacca gcgtaatcag cgccaccaac
    31081 aggtgcatgt agaggaatga cagtccgctg acttcgcaaa ccagccatag cccggcaaac
    31141 atgatggtga tatctgaaaa gcgttgcacc atagagatta acgatgcatt ggttttcgct
    31201 cgctcgcgct tttttagatt tgtcatcgtt gttcctgtta ttagcccctt acccgcagtg
    31261 gggtaaggga agatccgaca ttactcgttc agcaacgtca gcagagttcg cgttcgcgct
    31321 tccatcagcg gcacatcacc gcgcgattcc acattcaggc gcaccaccgg ttcggtattg
    31381 gaggtgcgca ggttaaagca ccagtcggca aaggtcatgc tgatgccatc gctgcgatcc
    31441 accgccagcg cctcacggct aaaatgctgt tccacgcggt taatcgcctc aacgggttgc
    31501 gccagtttgc tgttgatctc accgcttgcc ggaaacgccg ccatccggtc gcgcaccagt
    31561 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    31621 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc gccaccgtag
    31681 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacattacc
    31741 ggcgtgccgc ctgcagcagt caccacatca atagtgttcc aagagagacg cggatcatgg
    31801 atgatcttcg cgccgggatt tttttcgagg aacgcttctg ccagcaggcc gacaatgtag
    31861 tagccctcga taaactgccc tttttcgtca aacagaaaac agcggtcaaa atcgccatca
    31921 aaggcaatgc ccatatccgc gccgtgtttg atgaccgcat tgcgggtgtc gtcgcgacat
    31981 tccggcagca gcgggttagg aataccgttg gggaaattgc cgtccggtgt gttgtgtact
    32041 ttgattaatt ccacgggcgc gccgagggct ttaaagcggg cttcaatggc gtccaccacc
    32101 ggacccgctg cgccattccc ggagttgatc accagcttga gcggcgtgag atttttgaca
    32161 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca ggttgatttg ctgatagcga
    32221 ccgcgtttgg tttcatcgac gggaggaaag tcgttggctt cagccagacg ctggacgtcg
    32281 cgcagtccgg tatcaccgct gatcgtgcga gccccctcgc gcaccagctt catgccgttg
    32341 taatccatcg gattatggct ggcagtaact tcaatgccgc catccacgcc gagatggaac
    32401 gtggcgaaat agatctcttc ggtgccggac ataccgatat ccagcacatc gacgcccgca
    32461 tcctgtaaac cttttgccag cgccagtttt aaggtttcgc tggtgagacg gacatcaccg
    32521 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    32581 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    32641 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    32701 tttatttttg tcgttattta caccgcggtt tcgcattcat tgcctgatgc gacgctgccg
    32761 cgtcttatca ggcctacaaa accgaaccgt aggtcggata aggcgttcac gccgcatccg
    32821 acaaacagcg cctgatgcga cgtttacacc cgtccgtagc gatccgcgaa gcgcaccaca
    32881 tcatcctctt cgagataaga gccggagcgc acttcaatta aatcgagcgg aattttcccc
    32941 gggttttcca ggcagtgcgt cgcccccagc ggaatataaa tggactcgtt ttcaccaagc
    33001 agtttgatat caccatcaat ggtgactttt gccgttcccg cgaccactac ccagtgttcc
    33061 gcgcggtgat ggtgcatctg taccgacaaa ccctcgcccg gtttcacggt gatgcgtttc
    33121 acctggtagc ggtcgcccgc gtcgatagag tcatatttgc cccacggacg atacacttcg
    33181 cgatgcaccc gatgctcatg gcgaccatcg gctttgatct gctcgaccac ttttttcaca
    33241 tcctgtaccg cgttacggtc ggcaatcagc accgcatctt tggtctgcac cactaccaga
    33301 tctttcaccc cgacggtggt gaccaggcct gattcggcat acacatagct gttttcagtt
    33361 ttgtgattaa tcacatcgcc gtggcaaacg ttgccctcgg cggtgtgggc gctgatctcc
    33421 cataatgaag accaggaacc aacatcgctc cagcccgcat ccatcggcac cacaacagca
    33481 tctgccgtag gttccatgac cgcgtaatcc accgactctt ccgggcaggc gagaaacgct
    33541 tcttcatcca cacgaataaa atcgagatcc ggatcgacgg cgctcatcgc tttttcgcag
    33601 gcatcgagga tatccgggcg atattttttc agttcttcga gatagcgtcc ggcgcggaac
    33661 aggaacatgc cgctgttcca gtaatattcg ccgcttgcta cataggcctg ggcggtttcc
    33721 agattcggtt tttcgacaaa ctgcgccact tcaaaggcca ccgtatcctg ctcacccaca
    33781 gacacttcac cgcgacgaat atagccataa ccggtttctg gtagatccgg cacaatgcca
    33841 aaggtcacca gcttgcccgc ttcggcataa ggcatggcat tacgcacggc ggcacggaac
    33901 gcgtcttcat cggcaatcac atgatccgcc gccaatacca gcattaacgg gtcgctctcc
    33961 gggctatgac gttttgccgc cagcgccgcc agtgcaatgg caggtgcagt gttacgccct
    34021 gccggttcga gaataatgtt ctcggtgagt ttgttcagtt gacgcagctg ttccgcgaca
    34081 ataaagcggt gctgctcatt gcaaatcacc accgggcttt cgcactccac gccgttcagg
    34141 cggcagatgg tggtttgcag catggtgaga tcgcctttca ggcataaaaa ctgcttgggg
    34201 taaagtacgc gggaaagcgg ccataaacgg ctaccggagc cacctgccat cacaactgga
    34261 tagagtttcg actgcgccat aattatcccc gaatatcatt tataaattga cgtaacacgt
    34321 tctctttatc gagcgtgcgt tcggcatatt cacgtgccac cgtgttatgt ttgggcagca
    34381 ggagcgcctg acgaatcccc gccaccagcg cctcgaccga ttccggttct acgcaaaccg
    34441 caatacccgg aaaggtttcg caaagctgcc ccagttctgt gtgggcctct gcggtaatca
    34501 ccgcgttacc gcctaccgcc agaatattgg tcagtttcga cggcaatacg gcatctgccg
    34561 cgccgcgttt ttgcaccacc agatggcaat cgcccatctt cagcagtgca ggtaaagcgt
    34621 catacgattg cagcgggaaa aattgcatgt tgcgcagtcc acgctgctgc gccatttttt
    34681 ccagccgcgc tttgccgccg ctctgcccga caatggcaaa aatcagcggt tcatcgcgca
    34741 ggagatcggc agcttcaata acgttttcca gcccctgctt ttcaccaata ttgccggagt
    34801 aaagaatgat ttttttgtta tccggcaggc caagctggtt acgaagggca tcaacatcgg
    34861 cgtctgcaac atgctgaaaa cgggcaattt ccgaccagtt ggggaagaag atgacgttat
    34921 ccgccgccac gcctttttcg atggctttat tcatcatcga acgcgaaatc gtggaaacgt
    34981 tatcgacgtt atgcagtccg ctacgttcga acgccgttgc cagctgtgcc actttgccgc
    35041 ctttgccttt tccggcaagg cccagcccca gcatggcgtc cacttcgtaa tcctgaatat
    35101 gcagcacggt acgcgcacca gagagcttcg ccagcaggcg cattcccggc gtgcaaaaca
    35161 gcgttggcac tacgccaata atgcgatccg gcttccagcg acgttgcgcc atcagtggaa
    35221 agaaactgct gacggcaaaa ctgccgagat gcaacaagcg tttcagggtg ctcggctgtt
    35281 ttggcacgta cagcgggcag cgccacaccg tggcagcccc ttcttctcgt ttgtagcgcc
    35341 aggcggaata gttctcaccc acctgccatt gcgggtagta aggcggtgcg gtaatgaccc
    35401 gcacctcatg accttgtgcc gccaaccatt ccaccatctc gccggtgtat ttgccgatgc
    35461 cggttaactc cggcgagtag ttaatgccgt agaccagtat tttcataatc cgggtactcc
    35521 ggcacgcttc tcagcgagaa aataggctcg gctgttagcg tgaacatcgt tactggcgag
    35581 cagcgcgtcc ggcgtcagcc agcggtaatc gtcatgctgc tcatccggca gtaacagctc
    35641 ttcttccgct actctgaagc gaaaaccgag caccacatag tgagtggtga aatccgtgcc
    35701 ggagaagtta tcgtcataaa aatgctgcca gacaccgtaa aactggcctg ctgttatcgg
    35761 cagacgcagc cccagttccg ccatcgtcag ccgctcaaat gcggcttcca gcgtttcgtc
    35821 tttctgcacg cgccctcccg gcacaaacca gtaaccctgc gccgggcggt tggttctttt
    35881 gccaagcaga aactcgccgc gactgttctc gacaataaag tcgagagaga caagcggtgt
    35941 ggagcgcact acagtggcaa agtcttcctg acgtaaaaac atcattcccc ccgaaagcgg
    36001 tcttgattct caaggaacca ctggtaagtg ctggcaagcc ccgcttccag tgagatttcg
    36061 tgataccagc caagctgatg caggcgcgtc acatccagca gtttgcgcgg cgtaccatcc
    36121 ggtttgctgg catcaaaaac cacccgacct ttgtaaccca ccactttggc gatggtttgc
    36181 gccagctcgc ggatagtgca gtcaacgccc gtgccgacgt taatgtgcga cagcatcggt
    36241 tgggtattct ccagccagac ttcatgcgcc agctccatga catgaatgct cgccgccgcc
    36301 atatcatcga cgtgcaggaa ttcacgcatc ggtgtaccgc tgccccatac caccacgtcc
    36361 gtgccgtcgc ctcgtggaag cgacgcagca atgctggtat cacatgggag ttactcgggt
    36421 ggaagttgtc atgcggcccg tacaggttgg tcggcatgac agtgcggtaa tcgcgtccat
    36481 actggcggtt gtaagattcg cacagtttga tcccggcgat tttggcaatg gcgtaaggct
    36541 cgttggtcgg ctccagcgtg ccctgcaata actcgctttc tgccatcggc tgttttgcca
    36601 gtttcgggta gatgcaggac gatccgagaa acatcagttt gttcacgtcg ttctgatgcg
    36661 cggcgtgaat gatgttgctc tcaatcatca tgttctggta gatgaaatcc gccggatagg
    36721 tgttgttggc aacaatgccg cccactttcg ccgccgccag atagacctgg tcaatgcgtt
    36781 cgctggcaaa gaaatcatgc acggcgcggc tgtccagcag gttcagttca tcgcgggtac
    36841 gtaataccag ctccacatca ccgcgctgtt cgagctgccg cttgatggca gaaccgacca
    36901 tcccacaatg gccagcgata aaaatgcgtt gtttactcat gcttatgact ccagcgcgat
    36961 cgccacgtcg tagccgtgag atttcagcag agagtgtttt ttcgccgctt cgaggtcatt
    37021 agccaccatt tcagacacca tctctctgag ggtgatttcc ggtttccagc ccagtttttc
    37081 gtgcgctttg gtcgggtcgc cgagcagcgt ttcaacttct gccggacgga agtaacgcgg
    37141 atcaacggcg ataatcacat cacccggttt aacgcccggc gcgtcatgcc cggtgacgga
    37201 aaccacaatg cccttctctt caacacccgt gccttcaaag cgcagtttga tgcccagctg
    37261 tgctgccgcc atttccacaa actgacgtac ggagtactga acgccggtcg cgataacgaa
    37321 atcttccggc tgttcctgtt gcagcatcat ccactgcatt tttacgtagt ctttggcgtg
    37381 gccccagtca cgcagggaat ccatattgcc gaggtacagg cacgactcca gcccctgggc
    37441 gatgttggcg attgcgcggg tgattttgcg ggtaacgaag gtttcgccgc ggcgcgggga
    37501 ttcatggttg aagagaattc cgttacaagc gtacatgccg taggattcgc ggtagttaac
    37561 ggtgatccag taggcgtaca gtttggcgac cgcatacgga gatcgcgggt agaacggcgt
    37621 ggtttctttc tgcggaattt cctgcaccag accatacagt tcagaggtgg aagcctgata
    37681 gaaacgagtt ttcttttcca gaccgaggaa gcggatcgcc tccagcaggc gcagcgtacc
    37741 catcgcgtcg acgtcagcgg tatattctgg tgactcaaaa gagaccgcaa cgtggctcat
    37801 tgcgcctagg ttgtacactt catccggctg tacttcacgc aaaatgcgcg tcaggttaga
    37861 ggtatcactc aggtcgccat aatgcagatg gaatttcggg ttgcaggtgt gcggatcctg
    37921 ataaatgtga tccacgcgct cggtgttgaa tgacgatgcg cgacgcttaa taccatgcac
    37981 ctcgtaacct ttttccagca gaaactctgc caggtaagaa ccgtcttgtc cggttacacc
    38041 ggtgatgaga gcgacttttg acatgtctta ttcctctgta tttttgaatt tattcagttt
    38101 caacgcgttc gcgtatcacc actgcgggat tcccccggca aaccacattt gccggaagcg
    38161 atttaaaaac actgcttcgt gcacccacga cggtgccgtc gccgattgtg acgcctgggg
    38221 caacaaagac atcggttgcc agccagcatt tctcgccaat cacaataggc gtggcgttaa
    38281 tggtgaaatg ttgacttgca tggtcgtggc taccggtgca taaataactt ttttgcgata
    38341 tcaccgaatg tgcgccaatg gttatttcac cgagggtata taaattgacg tcatcgccaa
    38401 cccacgcgta atcacctaag gttaatttcc acggataggt aatttttact gacggacgaa
    38461 taactacgtt ttttcctatt tttgctccga ataaacgtaa taaaaaagcc cgccagcgat
    38521 acaatacttg tggcgaccag gcaaatattg ttgcctgtac tgcccaccat aattgcactt
    38581 taatagcgtt gccgccccgg aaccctttcg gcaccgagaa accgcttaaa tcttgcattg
    38641 gttttcctta tattcagact ttgttatata aggctttagt cttacctgtc gtacgttggc
    38701 gtaaatgcca ggataattct gcccagaagc caggtacatg taatatctgc cgttggactt
    38761 ttttggcgtc agca
//
LOCUS       DAEEGA010000031        38531 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEEGA010000031 DAEEGA010000000
VERSION     DAEEGA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38531
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.031"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..74)
                     /locus_tag="KLH46_001725"
     CDS             complement(<1..74)
                     /locus_tag="KLH46_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HBE0378437.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="KLH46_001726"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="KLH46_001726"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBE0378438.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="KLH46_001727"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="KLH46_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBE0378439.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="KLH46_001728"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="KLH46_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBE0378440.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="KLH46_001729"
     CDS             2704..2940
                     /locus_tag="KLH46_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBE0378441.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="KLH46_001730"
     CDS             2943..3800
                     /locus_tag="KLH46_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE0378442.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5797
                     /locus_tag="KLH46_001731"
                     /pseudo
     CDS             3800..5797
                     /locus_tag="KLH46_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5798..6319)
                     /gene="sodC"
                     /locus_tag="KLH46_001732"
     CDS             complement(5798..6319)
                     /gene="sodC"
                     /locus_tag="KLH46_001732"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBE0378443.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6400..7296)
                     /locus_tag="KLH46_001733"
     CDS             complement(6400..7296)
                     /locus_tag="KLH46_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBE0378444.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7345..7584)
                     /locus_tag="KLH46_001734"
     CDS             complement(7345..7584)
                     /locus_tag="KLH46_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBE0378445.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7687..8286
                     /gene="nemR"
                     /locus_tag="KLH46_001735"
     CDS             7687..8286
                     /gene="nemR"
                     /locus_tag="KLH46_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBE0378446.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8323..9420
                     /gene="nemA"
                     /locus_tag="KLH46_001736"
     CDS             8323..9420
                     /gene="nemA"
                     /locus_tag="KLH46_001736"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBE0378447.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9501..9908
                     /gene="gloA"
                     /locus_tag="KLH46_001737"
     CDS             9501..9908
                     /gene="gloA"
                     /locus_tag="KLH46_001737"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBE0378448.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10011..10658
                     /gene="rnt"
                     /locus_tag="KLH46_001738"
     CDS             10011..10658
                     /gene="rnt"
                     /locus_tag="KLH46_001738"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBE0378449.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10751..15209
                     /locus_tag="KLH46_001739"
                     /pseudo
     CDS             10751..15209
                     /locus_tag="KLH46_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15253..16461)
                     /locus_tag="KLH46_001740"
     CDS             complement(15253..16461)
                     /locus_tag="KLH46_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0378450.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16469..16969
                     /gene="tnpA"
                     /locus_tag="KLH46_001741"
     CDS             16469..16969
                     /gene="tnpA"
                     /locus_tag="KLH46_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBE0378451.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     THRIKD"
     gene            complement(17008..17355)
                     /gene="grxD"
                     /locus_tag="KLH46_001742"
     CDS             complement(17008..17355)
                     /gene="grxD"
                     /locus_tag="KLH46_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBE0378452.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17690..18517
                     /gene="mepH"
                     /locus_tag="KLH46_001743"
     CDS             17690..18517
                     /gene="mepH"
                     /locus_tag="KLH46_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBE0378453.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPHTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18645..19226
                     /gene="sodB"
                     /locus_tag="KLH46_001744"
     CDS             18645..19226
                     /gene="sodB"
                     /locus_tag="KLH46_001744"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBE0378454.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19372..20541)
                     /locus_tag="KLH46_001745"
     CDS             complement(19372..20541)
                     /locus_tag="KLH46_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0378455.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20707..20796)
                     /gene="ynhF"
                     /locus_tag="KLH46_001746"
     CDS             complement(20707..20796)
                     /gene="ynhF"
                     /locus_tag="KLH46_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBE0378456.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            21095..22120
                     /gene="purR"
                     /locus_tag="KLH46_001747"
     CDS             21095..22120
                     /gene="purR"
                     /locus_tag="KLH46_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBE0378457.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22117..23049)
                     /locus_tag="KLH46_001748"
     CDS             complement(22117..23049)
                     /locus_tag="KLH46_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0378458.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            23162..24373
                     /locus_tag="KLH46_001749"
     CDS             23162..24373
                     /locus_tag="KLH46_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBE0378459.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24664..25812
                     /gene="cfa"
                     /locus_tag="KLH46_001750"
     CDS             24664..25812
                     /gene="cfa"
                     /locus_tag="KLH46_001750"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBE0378460.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25852..26493)
                     /gene="ribE"
                     /locus_tag="KLH46_001751"
     CDS             complement(25852..26493)
                     /gene="ribE"
                     /locus_tag="KLH46_001751"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBE0378461.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26708..28081
                     /gene="mdtK"
                     /locus_tag="KLH46_001752"
     CDS             26708..28081
                     /gene="mdtK"
                     /locus_tag="KLH46_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBE0378462.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28122..29378)
                     /locus_tag="KLH46_001753"
     CDS             complement(28122..29378)
                     /locus_tag="KLH46_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBE0378463.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29686..29762
                     /locus_tag="KLH46_001754"
     tRNA            29686..29762
                     /locus_tag="KLH46_001754"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29720..29722,aa:Val,seq:gac)
     gene            29767..29843
                     /locus_tag="KLH46_001755"
     tRNA            29767..29843
                     /locus_tag="KLH46_001755"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29801..29803,aa:Val,seq:gac)
     gene            29951..30256
                     /locus_tag="KLH46_001756"
     CDS             29951..30256
                     /locus_tag="KLH46_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBE0378464.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30382..31986
                     /locus_tag="KLH46_001757"
     CDS             30382..31986
                     /locus_tag="KLH46_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBE0378465.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(31998..32810)
                     /locus_tag="KLH46_001758"
     CDS             complement(31998..32810)
                     /locus_tag="KLH46_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378466.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32814..33599)
                     /locus_tag="KLH46_001759"
     CDS             complement(32814..33599)
                     /locus_tag="KLH46_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBE0378467.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33596..34264)
                     /locus_tag="KLH46_001760"
     CDS             complement(33596..34264)
                     /locus_tag="KLH46_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBE0378468.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34328..34966)
                     /locus_tag="KLH46_001761"
     CDS             complement(34328..34966)
                     /locus_tag="KLH46_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBE0378469.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34979..37080)
                     /locus_tag="KLH46_001762"
                     /pseudo
     CDS             complement(34979..37080)
                     /locus_tag="KLH46_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(37101..37727)
                     /locus_tag="KLH46_001763"
     CDS             complement(37101..37727)
                     /locus_tag="KLH46_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBE0378470.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(38183..38392)
                     /gene="fumD"
                     /locus_tag="KLH46_001764"
     CDS             complement(38183..38392)
                     /gene="fumD"
                     /locus_tag="KLH46_001764"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBE0378471.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         9281 a        10056 c         9432 g         9762 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccggct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgcaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttacttttgg tcaaatttgg cctgatggtc cagattagcg atctgtggca
     5101 atttttactg tttctctttc cactgctggc gacaatgcag cttcttaaat tgcagatgcc
     5161 caaatttgcc gcactgtggg ggcaactgat tgtttttatg ggttcgttta tcgctgtcac
     5221 taatcccccg gtgtatgatt ttgctgattt tcttaactat aatctggcaa aaatcgttgg
     5281 cgttgcgctg gcttggctag cgttcgccat tctgcgtcca ggatcggatg ctcgtaaaag
     5341 ccgccgccat attcgcgcgc tgcgccggga ttttgtcgat cagctaagcc gccatccaac
     5401 actgagtgaa agcgaatttg aatcgctcac ttatcatcac gtcagtcagt tgagtaacag
     5461 ccaggatgcg ctggctcgcc gttggttatt acgctggggt gtagtgctgc tgaactgttc
     5521 tcatgttgtc tggcaattgc gcgactggga atcgcgttcc gatccgttat cgcgagtacg
     5581 ggataactgt atttcactgt tgcggggagt gatgagtgag cgtggcgttc agcaaaaatc
     5641 actggcggcc acacttgaag aattacagcg gatttgcgac agccttgccc gtcatcatca
     5701 acctgccgcc cgtgagctgg cggcaattgt ctggcggctg tactgctcgc tttcgcaact
     5761 tgagcaagca ccaccgcaag gtacgctggc ctcgtaatta cttaattaca ccacaggcat
     5821 agcgttcacc gccaccgccc agcggtttag gttgatcgga catattatcg ccgccaacgt
     5881 ggaccatcag cgccttgtct ttgatttcat ccagtgattt cagacgaggc gcgatgacgg
     5941 catcggtagc tttgccgtca ttattgacga ccagtgcagg cagatcgcct aaatgcccgg
     6001 taccttctgg cccttcatgt ttaccggtat tttgtggatc aagatgcccg cctgcggatt
     6061 ccgcggcgct ggctttgcca tctttggtgg ctggctggca gcttcctttg gcatgaatat
     6121 ggaagccatg ttcaccgggg ggtaatgctt tcagatcggg tgaaaactcc agacctttat
     6181 cggtttcagt aatggtgacg ctaccaattg actgcccaac cccttgcgac gtgacgaggt
     6241 tcatctcgac tttttcactg gcagcttgtg cgccggtcgc aacaaccagc gccagaatgg
     6301 ccagactaaa acgtttcata ggacctccgt tcattgtgca ttcctgaata agtgtaaacc
     6361 agtgacacat ttttgaacgg cggctattcc taaaagtgct tacggtacgt cgtaccccag
     6421 tgcagcttta cggatacgaa accattgttg acgggtcatt ttcagtgttt ctgcttcgac
     6481 agctgcccgt acgcgctcaa ttttacctga gccgataatt ggcagcggct gcgacggtaa
     6541 acgtaatacc caggcgtaaa ccacctgttc aatcgagccc gcgtttaact cctccgccac
     6601 cacagccagt tcatcacgca gcggctggaa ataatcatca ttaaacagac gaccaccacc
     6661 aaggcaggac cacgccatcg gacgaacacg cagttgttgt agttggtcga gcgtgccatc
     6721 cagcagtaac ggctgatgca ccggggatat ttccacctga ttagtggcaa gggtaaacgg
     6781 cagacgtgat tgcaacaggg caaattgcgc aggcgtaaag ttcgatacgc caaaatgacg
     6841 cactttgccg ctctgatgca gatgtttgaa cgcgtccgcc acttcatcgg catccattaa
     6901 cgggtctggt cggtggatta acagcagatc cagatgatcg gtcgcgagat taattagcga
     6961 ctgttcggcg ctcttaatga tgtgattgcg gtcagtgatg taatgaccaa tgacgttttc
     7021 ttcacgcgcg gtcgtcgcga taccgcattt actgacgatt tccatccgtt cacgcaggtg
     7081 aggtgccagt ttcagtgcct cgccaaacgc cgcttcgcac tgatagccac cataaatatc
     7141 agcatggtcc acggtggtca cgccgagatc cagatgctct tcaataaaac tgaccagctg
     7201 gcgggcggac atattccagt ccatcaatcg ccagtagccc atcacaaaac gggaaaactc
     7261 cgggccttcc ggcgcaatag taatacgctg aaccataatc gcttcctctt atcagatata
     7321 agaggagtat acgcaagatt aggttcaaaa gagtgatggt tgctccggtt cgtctgatga
     7381 cgctggctta tttgcgcgta atttgcgcat taatcgctgc cgacaaaggc gcagcacctc
     7441 ttgtttttcg ccatcgctca ttttattcca gttaaaacgc tcatcccgac tacgaaaaca
     7501 gccgcgacaa aacccgcgtt cgtcagactg gcaaattccc cggcacgggc tctggacggg
     7561 aaagaactct aattgctccg ccacctcgcc ctcctcagat aagattatta ccattattga
     7621 agctgttaat gtccaaagta gcaactttgc ttgcactaga ccgactggtc tactacactc
     7681 caacgcatga acaaacacac cgaacatgat actcgcgaac atctcttggc gacgggcgag
     7741 caactttgcc tgcaacgtgg attcaccggg atggggctaa gcgaattact aaaaaccgct
     7801 gaagtgccga aagggtcctt ctatcactac tttcgctcta aagaagcgtt tggcgttgcc
     7861 atgcttgagc gtcattacgc cgcatatcac cagcgactgg ctgagttgct gcaatccggc
     7921 gaaggtaact accgcgaccg catactggct tattaccagc aaacactgaa ccagttttgc
     7981 caacatggaa ccatcagtgg ttgcctgaca gtaaaactct ctgccgaagt gtgcgatctg
     8041 tcagaagata tgcgcagcgc gatggataaa ggtgctcgcg gcgtgatcgc cctgctctcg
     8101 caggctctgg aaaatggccg tgagaaccat tgtttaacct tttgtggcga accgctacaa
     8161 caggcacaag tgctttacgc actatggctt ggcgcgaatc tgcaggccaa aatttcgcgc
     8221 agtttcgagc cactggaaaa cgcgctggcc catgtaaaaa acattattgc gacgcctgcc
     8281 gtttagcagg cattttttat caccagacga ccgggagcct ttatgtcatc tgaaaaactg
     8341 tattccccac tgaaagtggg cgcgatcacg gcggcaaacc gtatttttat ggcaccgctg
     8401 acgcgtctgc gcagtattga accgggtgac attcctaccc cgttgatggc ggaatactat
     8461 cgccaacgtg ccagtgccgg tttgattatt agtgaagcca cacaaatttc tgcccaggca
     8521 aaaggatatg caggtgcgcc tggcatccat agtccggagc aaattgccgc atggaaaaaa
     8581 atcaccgctg gcgttcatgc tgaaaatggt catatggcct tgcagctgtg gcacaccgga
     8641 cgcatttctc acgccagcct gcaacctggc ggtcaggcac cggtagcgcc ttcagcactt
     8701 agcgcgggaa cacgtacttc tctgcgcgat gaaaatggtc aggcgatccg cgttgaaaca
     8761 tccatgccgc gtgcgcttga actggaagag attccgggta tcgtcaatga tttccgtcag
     8821 gccattgcta acgcgcgtga agccgctttt gatctggtag agctccactc tgctcacggt
     8881 tatttgctgc atcagttcct ttccccttct tcaaaccatc gtaccgatca gtacggcggc
     8941 agcgtggaaa atcgcgcacg tttggtactg gaagtggtcg atgccgggat tgaagaatgg
     9001 ggtgccgatc gcattggcat tcgcgtttcg ccaatcggta ctttccagaa cacagataac
     9061 ggcccgaatg aagaagccga tgcactgtat ctgattgaac aactgggtaa acgcggcatt
     9121 gcttatctgc atatgtcaga accagattgg gcggggggtg aaccgtatac tgatgcgttc
     9181 cgcgaaaaag tacgcgcccg tttccacggt ccgattatcg gcgcaggtgc atacacagta
     9241 gaaaaagctg aaacgctgat cggcaaaggg ttaattgatg cggtggcatt tggtcgtgac
     9301 tggattgcga acccggatct ggtcgcccgc ttgcagctca aagctgagct taacccacag
     9361 cgtgccgaaa gtttctacgg tggcggcgcg gaaggctata ccgattaccc gacgttgtaa
     9421 tccaacattg cgagcggcgt aaagccgccg ctatactaaa acaacatttt gaatctgtta
     9481 gccattttga ggataaaaag atgcgtcttc ttcataccat gctgcgcgtt ggcgatttgc
     9541 aacgctccat cgatttttat accaaagtgc tgggcatgaa actgctgcgt accagcgaaa
     9601 acccggaata caaatactca ctggcgtttg ttggctacgg cccggaaacc gaagaagcgg
     9661 tgattgaact gacctacaac tggggcgtgg ataaatacga actcggcact gcttatggtc
     9721 acatcgcgct tagcgtagat aacgccgctg aagcgtgcga aaaaatccgt caaaacgggg
     9781 gtaacgtgac ccgtgaagcg ggtccggtaa aaggcggtac tacggttatc gcgtttgtgg
     9841 aagatccgga cggttacaaa attgagttaa tcgaagagaa agacgccggt cgcggtctgg
     9901 gcaactaatc tcctgccggg cgtgaactca tcgcgcccgc atctttactg catcgacaag
     9961 taatatttgt cataatgcgc gctgcaattt atccgtatta agagaatcag atgtccgata
    10021 acgctcaact taccggtctg tgcgaccgtt ttcgtggttt ttatcctgtt gtgatcgatg
    10081 ttgaaacagc cggatttaac gccaaaaccg atgcgctgct tgagattgcc gccatcaccc
    10141 tgaaaatgga tgaacaaggc tggctgatgc cggacaccac attacatttc cacgtcgaac
    10201 catttgtcgg cgcaaatttg caaccagaag ccctcgcctt caacggcatt gacccgaacg
    10261 atcccgatcg cggcgcggtc agcgaatacg aggcgctgca cgaaattttt aaagttgtac
    10321 gtaaaggtat taaagcgagc ggctgtaacc gcgccattat ggtggcgcac aacgccaatt
    10381 ttgatcacag ctttatgatg gccgccgcag aacgcgcctc gctgaaacgt aacccgttcc
    10441 accctttcgc cacttttgac actgctgcac tggccgggct ggcactcgga caaaccgtat
    10501 tgtcaaaggc ttgccagacc gctggcatgg acttcgacag cacccaggcg cactccgcgc
    10561 tgtacgacac cgaacgcact gctgtgctgt tttgtgaaat cgtcaaccgc tggaaacgtc
    10621 tgggaggctg gccgctacct gccgccgaag aggtgtaatc gagtcgatgc ctgatgacat
    10681 gcaatgattc aggcatctat agtgaggcta ttccacgcat caagcatgat attcacgggg
    10741 aatagcgtta atggcagata atccagaccc ttcatcgctc ctgccggacg tgttttcacc
    10801 ggcgacccgc gactggtttc ttcgcgcctt taaacagccg accgctgtcc agtcgcaaac
    10861 ctggcatgtg gcggcgcgag gcgaacatgc gctggtgatt gcaccgactg gctccgggaa
    10921 aacgctggca gcatttctct acgccctcga tcggctcttc cgcgaaggcg gcgaagatac
    10981 ccgcgaggcg cataagcgta aaacctcacg catcctctat atttcaccga taaaagccct
    11041 gggcaccgac gttcagcgca acttgcagat cccgttgaag ggtattgccg atgaacggcg
    11101 gcggcgcggc gaaacggtaa ttattgatga agtccacgcg gtagcgggca gtaaacgtgg
    11161 tgcgcatctg gcgttaagtc tggagcggct cgatgcgctg ctccacacct cagcacagcg
    11221 aattggcctt tctgccactg tgcgctcagc cagcgatgtg gcagcatttc ttggtggcga
    11281 tcgcccggtt acggtagtca acccgcccgc aatgcgccat ccgcagatac gaattgtcgt
    11341 accggtcgcc aatatggatg atgtctcatc ggtcgccagc ggcaccggcg aagacagcca
    11401 tgccggccgg gaaggctcca tctggccata tattgaaacg ggtatccttg atgaagtgtt
    11461 gcgccatcgc tcgaccattg tctttactaa ttcacgcggg ctggcggaaa aactgacagc
    11521 acgattaaat gagctttacg ccgcacgctt acagcgttcc ccgtctatcg ccgttgatgc
    11581 ggcccatttc gagtcgacct ccggcgcaac ctctaaccgt gtacaaagta gcgacgtttt
    11641 tattgcccgc tcacaccacg gctccgtctc taaagaacaa cgagcaatca ccgaacaggc
    11701 gctgaaatcg ggtgaattac gctgcgtggt cgcaacctcc agtcttgaac tggggattga
    11761 tatgggcgcg gtggatctgg tgattcaggt ggcaacgccg ctttctgttg ccagtgggtt
    11821 acaacgcatt ggtcgcgccg gacatcaggt tggcggtgta tctaaagggc tgtttttccc
    11881 ccgtacccgg cgtgatttag tcgattccgc agtcattgta gagtgtatgt tcgcaggcag
    11941 gctggaaaac ctgacaccac cgcataatcc tctcgacgtc cttgcgcagc aaaccgttgc
    12001 cgccgcggcg atggatgcat tacaggtaga cgaatggtac tcccgcgtac gccgtaccgc
    12061 accgtggaaa gatctgccaa gacgtgtttt tgacgccacg ctggatatgc tttccgggcg
    12121 ctatccctct ggcgattttt ctgcttttcg ccccaaactg gtctggaaca gggagaccgg
    12181 gatattgacc gcccgacctg gcgctcaatt gttggcggtt accagcggcg gcaccattcc
    12241 ggatcgtggc atgtatagcg tgttattacc cgaaggtgaa gaaaaggccg gttcgcggcg
    12301 ggtgggtgaa ctggatgagg agatggtata tgagtcgcgg gtgaacgaca ttatcactct
    12361 cggcgctacc tcatggcgga tccagcaaat cacccgcgat caggtgattg tgactcctgc
    12421 tccgggtcgt tctgcccagc tccccttctg gcgtggtgaa ggtaacggac gtccggctga
    12481 attaggcgag atgatcggcg attttcttca tttactggcg gatggcgcgt tcttttccgg
    12541 gactattccc ccgtggctgg cagaagaaaa tacgaacgcc aatattcagg ggttgattga
    12601 cgagcaacgc aacgcgacgg gcatcgttcc ggggagtcgc catctggtcc tcgaacggtg
    12661 ccgtgatgaa attggcgact ggcgtattat tttgcactct ccctatggaa gacgggtgca
    12721 tgaaccctgg gcgctggcga ttgccgggcg aatacatgcc ctatggggcg ctgacgcgtc
    12781 tgtggtcgcc agtgatgacg gcattgttgc gcgcattcct gacactgatg gtaaattgcc
    12841 cgacgccgcg atttttttgt ttgaaccaga aaagttgctg caaattgtcc gcgaggcggt
    12901 aggcagctcg gcacttttcg ccgcccgttt tcgcgaatgc gccgcgcggg cattattaat
    12961 gccgggccgc acgccgggcc atcgcacccc gctttggcaa caacggctgc gcgccagtca
    13021 gttgctggaa atcgctcagg gatatccgga ttttccggtc attctcgaaa ccctacgcga
    13081 atgtctgcaa gatgtttatg atcttcccgc actggaacgt ttgatgcgtc gcctgaacgg
    13141 tggcgaaatt caaatatccg atgtaacgac cactacgccc tcgcctttcg ccacaagttt
    13201 attgttcggc tatgtcgcgg aatttatgta ccagagcgac gccccgctgg cagagcgccg
    13261 ggcatccgta ctgtcgctgg acagcgagtt actgcgcaat ctactcggac aggtcgatcc
    13321 gggggaatta ctcgacccgc aggtcattcg ccaggtggaa gaagagttgc aacgactggc
    13381 tcctggtaga agagcgaaag gtgaagaagg attgttcgac ctgctgcgcg aactggggcc
    13441 aatgaccgtt gaagacctgg cgcaacggca tacaggcagc agtgaagagg ttgcgtcgta
    13501 tctggaaaat cttcttgcag taaaacgaat cttcccagcg atgattagcg gacaggagcg
    13561 tcttgcctgt atggatgatg ccgccaggct gcgtgatgcc ctcggcgtac ggctaccaga
    13621 gtcattgcca gagatttatt tacatagagt cagttacccg cttcgcgacc tctttctgcg
    13681 ctatctccgg gctcatgctc tggtcacggc tgaacaactg gctcatgagt ttagtctcgg
    13741 tattgccatt gtcgaagagc agcttcagca actgcgtgaa cagggtctgg tgatgaatct
    13801 gcaacaaaac atctgggtga gcgatgaagt atttcgtcgt ctgcgtttgc gctcgctgca
    13861 agccgccaga gaagcgacgc gtcccgttgc agccacgacc tatgcgcgat tgctgctgga
    13921 acgtcagggc gtattacccg ccaccgatgg tagcccggcg ctctttgcct caacatctcc
    13981 aggcgtttat gagggcgtag atggcgtgat gcgggtgatc gaacagcttg ccggagtcgg
    14041 tttacccgcc tcactctggg aaagccagat cctgcctgcc cgcgtacgcg actattcgcc
    14101 agaaatgctc gatgaattac tggcaaccgg agcggttatc tggtcgggac aaaaaaaagc
    14161 tgggtgaaga tgacggcctg gtggcactgc atctacagaa atatgccgca gaatcgttca
    14221 ctcccgccga agcggatcag gcgaatcgtt cggcgctgca gcaagcgata atcgctgttc
    14281 tggctgacgg aggagcctgg tttgcacaac aaatcagcca acggatacgc gataaaatcg
    14341 tcgaatcggt tgattcctcg gcactgcaag aggcgttatg ggcgctggtc tggcaaggcg
    14401 tcattaccag cgatatttgg gcaccgttac gcgccctcac ccgcagcagt tccaacgcac
    14461 gcacctcaac tcgccgcagt caccgggctc gtcgtgggcg tcctgtctat gcacaacccg
    14521 tctcgccgcg ggtatcttac aacacaccaa atctggctgg acgctggtcg ttattgcagg
    14581 tggagccact aaacgatacc gaaaggatgc tggcgctggc ggaaaatatg ctcgaccgct
    14641 acggcatcat cagtcgtcag gcggtgatag ccgaaaatat ccctggcggg tttccatcga
    14701 tgcaaacact ttgtcgaagt atggaagact ccgggcgaat tatgcgaggt cgttttgtag
    14761 aaggtctggg tggcgcgcaa ttcgctgaac gtctgactat tgaccgattg cgcgatctgg
    14821 cgacacaagc cacgcaaaca cgccactata caccagtggc gctctccgcc aacgatccgg
    14881 ctaatgtgtg gggaaatctt ctgccctggc ctgcacatcc ggcaacgctg gtaccaacgc
    14941 gtcgggcggg tgcgctggtg gtcgtttctg gcggcaaatt gttactctat ctggcgcaag
    15001 gcggcaaaaa aatgctggtc tggcaggaaa aggaggaatt actcgcccca gagattttcc
    15061 aggcgctgac caccgcactg cgtcgcgaac caaggctgcg ctttacgcta acagaagtga
    15121 atgatcttcc ggtccggcaa acgccgatgt ttacgctgct gcgtgaggcg ggattttcaa
    15181 gttcgccaca agggctggat tggggatagt tgacatcctc cacgccctga aagacgtggt
    15241 tccctgctac gttcaagctg tcgcctgaat catttcggtg ggttcctgct tcaacgagcg
    15301 gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta aaatgttacg
    15361 agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga atttactttg
    15421 tgacaggcgg ttttctttcg ctgtatgacc acagcacgcg caacgctggc ttgtgtacgc
    15481 tggcggaaca gcaagcacct gaccgccact ccagagctgc ttgtactcaa gctggcggcg
    15541 catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg cccggacatt
    15601 gcgccccggc tggctgaccg tacccgctgc tgactttgac atgttgctga ccttcaaatc
    15661 ctcaatgact atcattgcgt ggtttttgct gacggtcgtt gtgactttgt gaaggtagtc
    15721 cctgcggata tttgcgatac aggaatgcag tcgctgtatt ttgcgtttct gcttctgcca
    15781 gttgttgctg aatttgacct tccggcttaa ctgtcgctga agtctcgcca gcgtcttctg
    15841 gtttttttga aaactgttta caggctcaaa gactgtgcca tctgacagcg tggcgagttt
    15901 agccacgcca gcatccagcc cgatcattga tgctgaaggg tgaaccggag ttgatacttc
    15961 actttctgtc tgaatactga tgtaccacgt accgcaggac tggctgacag tgacattttt
    16021 cacaactccc gtgacctgac ggctattccg gtagcgcatc cagccaagtt tcggcagaaa
    16081 aatacggctg ttttcctgat cgagcttaac gtcctgcggg tagcggaatg cagcattctg
    16141 tccccgcttt ttgaatcggg gaaaagcagc ccgcttctgg aagaagtttt tgtaagcccg
    16201 ctcaaggtct ttcagtgact gttgcaatgg ctgtgagggc gaatctttaa gccattgcgt
    16261 ttcagtggcg tttttccact caaccagcca ggaagccatt ttcccgtaag ggatgtattt
    16321 attaccggcc tcatgattct cattctgaag cgccagagca cgattgaaaa cgaaacgaca
    16381 tgcgcctgcg aagcgcctca tttcacgctc ctgttgacca cctggtctta actggaattt
    16441 aaatgcctgt aatcgtttca caggtcaaat gatactttgg tctgtgaaaa aagaaaccga
    16501 tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt cacctggtct ttgtcaccaa
    16561 ataccgacgc cagatttttg accatgacac gacagaaaaa ctacgcactt acttttcaaa
    16621 tgtatgtgct gattttgcag ctgaactggt tgaaatggat ggcgaaccag atcacgtcca
    16681 tttgttaatt aactatcctc ccaaactggc gatatccagt ctggtaaaca gcctcaaagg
    16741 catatcgaat aggttactgc gacgagatcg accagatatt gcagtcaggt attactacaa
    16801 aggcgttttg tggagtcctg gctattttgc cagtagctgc gaaggtgcgc caatatccgt
    16861 catccgccag tacattgaac aacagcaaac acctggtcag gtggaaaacc gcgccttata
    16921 tccccgccct gaaggacgag gttttacgac gcaccggata aaggactgac ggatgcccgt
    16981 tcgcatccgt cagtattgca ggacggatta ttccgcgtcc ggctcttcag acttgtattt
    17041 agcggcagtt tctttgatca gctgctgcag ttcgccacgc tgatacattt cgatcacgat
    17101 atcacaaccg ccgaccagct cgccgtcaac ccacagttgc gggaaggtcg gccagttagc
    17161 atatttcggc agttccgcac gaatgtccgg attctgcaga atatcaacat aggcaaaacg
    17221 ttcgccacat gcggcaagcg cctggactgc ctgggcagag aaaccgcagc tcggcagttt
    17281 cggtgaacct ttcatgtaca gcaggatcgg gttttcagca atctggcgtt ggattttttc
    17341 gatagtggtg ctcattgtct tgcttcctca aacttcttta cggcagtaat ctgacattgt
    17401 agcgggtcag tgcggcatcg gaaaataaca ttttcatcac gcttttgcta ttttatccct
    17461 ttgctctatt tgttgcattt caaatattcg tttcttctct gatgcgcggc aaactggcgt
    17521 atttataacc atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat
    17581 tttttaacaa taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt
    17641 gtaaatcaga cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat
    17701 aaaccgtatt tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat
    17761 ggcccatgct tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc
    17821 agataaaaag aaaagcacgg caaccaccaa aaagacccag aaagccagcc agaaaacagc
    17881 gaaaaaagcc gccagtaaaa gtatgaccaa aagcaaaacc gcttcttctg ttaaaaaatc
    17941 ttccattacc gcttctaaaa acgccaaaac tcgcagcaaa cacgccgtca ataaaacggc
    18001 ctcagccagc tttaccgaaa agtgtaccaa acgtaagggc tataaatcgc attgtgtgaa
    18061 agtcaaaaat gccgcgtcag gaacccttgc cgacgcgcac aaagcgaagg tgcaaaaagc
    18121 cacgaaagtg gcaatgaata aactgatgca gcaaattggt aagccatatc gttggggcgg
    18181 cagctcaccg cataccggtt ttgattgcag cggcctggtt tattacgctt ataaagattt
    18241 ggtgaaaatt cgtattccgc gtacggcgaa tgaaatgtat cacctgcgtg atgcaggacc
    18301 tatcgaacgt agtgagctga aaaacggcga cctggtcttt ttccgtactc agggacgcgg
    18361 tacagccgat catgtcggcg tgtatgtcgg caacggcaaa tttattcagt caccgcgcac
    18421 aggtcaggaa attcaaatca cttctctcag tgaagactac tggcagcgcc actatgttgg
    18481 cgctcgtcgg gtaatgaccc caaaaacact tcgctaaaac tttaccctgt tgttacggca
    18541 acagggtaag ttcatctttt gtctcacctt ttaatttgct accctatcca tacgcacaat
    18601 aaggctattg tacgtatgca aattaataat aaaggagagt agcaatgtca ttcgaattac
    18661 ctgcactacc atatgctaaa gatgctctgg caccgcacat ttctgcggaa actatcgagt
    18721 atcactacgg caagcatcat cagacttatg tcactaacct gaacaacctg attaaaggta
    18781 ccgcgtttga aggtaaatca ctggaagaga ttattcgcag ctctgaaggt ggcgtattca
    18841 acaacgcagc tcaggtctgg aaccatactt tctactggaa ctgcctggca ccgaacgccg
    18901 gtggcgaacc gactggaaaa gtcgctgaag ctatcgccgc atcttttggc agctttgccg
    18961 atttcaaagc gcagtttact gatgcagcga tcaaaaactt tggttctggc tggacctggc
    19021 tggtgaaaaa cagcgatggc aaactggcta tcgtttcaac ctctaacgcg ggtactccgc
    19081 tgaccaccga tgcgactccg ctgctgaccg ttgatgtctg ggaacacgct tattacatcg
    19141 actatcgcaa tgcacgtcct ggctatctgg agcacttctg ggcgctggtg aactgggaat
    19201 tcgtagcgaa aaatctcgct gcataataac tgatggcaaa tgcagcattg cctgatgcgc
    19261 ttcgcttacc atgccaacgc ggatgaggcg ttaacagcgc attcggcaag ataaatcgca
    19321 cgttgtcagc aactgtaacg cagaaggtta tccttctgcg tttttgttta attagctgtt
    19381 agcaacgcaa actgtttcag gttgttttct ggctgacata aacaccagca ataatgccag
    19441 tcccgcgaca atcgcccccg tcaccggcac aaagctgtat cccagcccag cggaaattac
    19501 cgcaccacca gcagctgctc ccagcgcatt tccaagatta aaggcaccaa tattgactga
    19561 tgaagacaga cctggcgctt cactggcgac atgcatcacg cgcatctgta acggaggtac
    19621 aaccgcaaag gttgctgcgc cccacaccac catgctaata gctgcgccga actcattgcg
    19681 ggccaggaac gggattgcca gcataatcac catcagcagc aacaaaaagc ctttcaacgt
    19741 gccgttaact gaacgatctg ccagtttgcc gccgagatag ttaccgatag agaatccgac
    19801 accaatcagc accagcattg ccgtgacgaa caccggtgtt gcgtgggtaa tactttgcag
    19861 taccggagag atataggtgt agagggtaaa cattgcacca gctcccagta ccgtcgtcag
    19921 caatgcagac agcacctgcg gacgcattaa taccgccagc tcttttttca cttcaggtcg
    19981 tgcccctgta ccacctttag gtaatgagaa gaacagactt accattgaaa tcactcccag
    20041 ccccgccgtt gccagaaatg acatccgcca gccgatggtt tcacccaacc aggtcgccgc
    20101 cggcacgcca ccgatatttg ccagggttaa ccccataaac atagtggcaa ctgcgctggc
    20161 ctgtttatgt tttggcacca cgcttgcggc cacgactgaa cccaaaccaa aaaatgctcc
    20221 gtgattcagg ctggtcaaaa tacgtgaaag catcagggtc atataatccg gcgcgatggc
    20281 ggaaagaacg ttgcctagcg tgaaaattgc catcaggaaa atcaacgcac tgcggcgggc
    20341 acgatgagaa agtagaagcg tcatcagcgg cgcgcccacc attacgccaa ctgcataggc
    20401 actgattaac attccggcag cgggaatcga gacatccaca ccgcgcgcaa tgacgggcaa
    20461 caagcccatt ggcgagaact ccgttgtccc gataccaaac gcgccaatcg ccagcgccag
    20521 caacggatag ttaattttca tgccttatct ccacctcttc gcgtcattac gcgatattca
    20581 ttaaagtggc gaaagcatga cagcaatcac aaaaaaatga aaataacaaa aagagaaaac
    20641 acttttgcca ttttgctaac aaacaggaag gagatgcgag ggagaacgcg ctccctcgag
    20701 aggaaatcag tgcagcgcgg cagtcaaacc cacggctacg atcaaaccga ggacgataat
    20761 cgttgttacc agtgaaaatt taaggtcggt gctcatcaag ttttctcctt ttttattacc
    20821 acacaaaaag tgatattacg catttttaca cactgtgatg aaaaaatctc ccgtcattta
    20881 taatgataag tgtttttacc acttcccctt ttcgtcaaga tcggccaaaa ttccacgctt
    20941 acactatttg cgtactggcc attgacccct tcctgacgct ccgtgtcgtt tttccggcgt
    21001 accgcaacac ttttgttgtg cgtaaggtgt gtaaaggcaa acgtttacct tgcgattttg
    21061 caggagctga agttagggtc tggagtgaaa tggaatggca acaataaaag atgtagcgaa
    21121 acgagcaaac gtttccacta caactgtgtc acacgtgatc aacaaaacac gtttcgtcgc
    21181 tgaagaaacg cgcaacgccg tgtgggcagc gattaaagaa ttacactact cccctagcgc
    21241 ggtggcgcgt agcctgaagg ttaaccacac caagtctatc ggtttgctgg cgaccagcag
    21301 cgaagcggcc tattttgccg agatcattga agcagttgaa aaaaattgct tccagaaagg
    21361 ttacaccctg attctgggta atgcgtggaa caatcttgag aaacagcggg cttatctgtc
    21421 gatgatggcg caaaaacgcg tcgatggtct gctggtgatg tgttctgagt acccagagcc
    21481 gttgctggcg atgctggaag agtatcgcca tatcccaatg gtggtgatgg actggggtga
    21541 agcaaaagct gacttcaccg atgcggtcat tgataacgcg ttcgaaggcg gctacatggc
    21601 cgggcgttat ctgattgaac gcggtcaccg cgaaatcggc gtcatccccg gcccgctgga
    21661 acgtaacacc ggcgcaggcc gccttgccgg ttttatgaag gcgatggaag aagcgatgat
    21721 caaggtgccg gaaagctgga ttgtgcaggg tgactttgaa cctgaatccg gttatcgcgc
    21781 catgcagcaa atcctgtcgc agccgcatcg ccctactgcc gtcttctgtg gtggcgatat
    21841 catggcaatg ggcgcacttt gtgctgctga tgaaatgggc ctgcgcgtcc cgcaggatgt
    21901 ttcgctgatc ggttatgata acgtgcgcaa cgcgcgctat tttacgccgg cgctgaccac
    21961 gatccatcag ccaaaagatt cgctgggtga aacagcgttc aacatgctgt tggatcgtat
    22021 cgtcaacaaa cgtgaagaac cgcagtccat tgaagtgcat ccgcgtttga ttgaacgccg
    22081 ctccgtggct gacggcccgt tccgcgacta tcgtcgttaa tcacccgttg cgggagtctc
    22141 ttccggctcc cgtagccact ccttattcag cgtctcacta tcgccgagat actcaagcag
    22201 ccaggttaac gcaggcgaca tatcattttg ctgccatgtc agacaacatg ccgaatccgg
    22261 gaaggggttt tccagttcaa gtgctaccca cttcccctca ttaagccacg gtttggcgaa
    22321 atgtgttggc accatcccta tgcataatcc tgccgagata caggttgccg atgattccca
    22381 gtcaggtacg acaagtcttt tttggttatc cagcaaccag gtaatacgtt taggtagcgt
    22441 tcgcgaggtg tcttcgcgca ccaacgacgg ccagttgcgc aacgtatcat cgctgaacgg
    22501 gccatccatc aacgccagcg ggtggtgact ggcaacaacg caactccagc ttagcatccc
    22561 catatcccgg aaggcataac gaccgcctac cggaatcgcg cgtgttgcgc caattgccag
    22621 ttccacgcgc ccgtcggaaa gcgcatccca gacaccgttg aacacttcct gaaagacaag
    22681 aagttcgaca tcatcaaaat ggcgataaaa atcaacgatc atctgccgtg tacgttctgg
    22741 cctgacaata ttatccactg cgatagctaa ctgaccgcgc cagccgttcg ctatctgctg
    22801 acattgctgg cgggtgatct gcattttttt gacaacagag cgcccttctt tgagaaacca
    22861 cgctccagca gcggtcagct ccacatcacg gtgccgtcgt tcaaagagcg gtaccgccag
    22921 ccactcttcc agctgacgca cggtatagct gaccgcagaa ggaacacgat gcagctcctg
    22981 tgccgcagcg ctaaaactac cattacgcgc taccgcatca acaacttcga gtgaatattc
    23041 tgaccacata gtctgcctgc aaaatttttg aaaccagtca tcaaatatta ccgtttcaca
    23101 acactaattt cactccctac actttgcggc ggtgtttaat tgagagattt agagaatata
    23161 catgcaacct gggaaaagat ttttagtctg gctggcgggt ttgagcgtac tcggttttct
    23221 ggcaaccgat atgtatctgc ctgctttcgc cgccatacag gccgacctgc aaacgcctgc
    23281 gtctgctgtc agtgccagcc ttagtctgtt ccttgccggt tttgccgcag cccagcttct
    23341 gtgggggcca ctctccgacc gttatggtcg taaaccggtg ttattaatcg gcctgacaat
    23401 ttttgcgtta ggtagtctgg ggatgctgtg ggtagaaaac gccgccacgc tgctggtatt
    23461 gcgttttgta caggctgtgg gggtctgcgc cgcggcggtt atctggcaag cattagtgac
    23521 agattattat ccttcacaga aagtcaaccg tatttttgcg accatcatgc cgctggtggg
    23581 tctgtctccg gcactggctc ccctgttagg aagctggctg ctggtccatt tttcctggca
    23641 ggcgattttc gccaccctgt ttgccattac cgtggtgctg attctgccta ttttctggct
    23701 caaacccacg acgaaggccc gtaacaatag tcaggatggt ctgaccttta ccgacctgct
    23761 acgttctaaa acctatcgcg gcaacgtgct gatatacgca gcctgttcag ccagtttttt
    23821 tgcatggctg accggttcac cgttcatcct tagtgaaatg ggctacagcc cggcagttat
    23881 tggtttaagt tatgtcccgc aaactatcgc gtttctgatt ggtggttatg gctgtcgcgc
    23941 cgcactgcag aaatggcaag gcaagcagtt attaccgtgg ttgctggtgc tgtttgctgt
    24001 cagcgtcatt gcgacctggg ctgcgggctt cattagccat gtgtcgctgg tcgaaatcct
    24061 gatcccattc tgtgtgatgg cgattgccaa tggcgcgatc taccctattg ttgtcgccca
    24121 ggcgctgcgt cccttcccac atgcaaccgg tcgcgccgca gcgttgcaga acactctgca
    24181 actgggtctg tgcttcctcg caagtctggt agtttcctgg cttattagta tcagcacgcc
    24241 attgctcacc accaccagcg tgatgttatc aacagtagtg ctggtcgcgc tgggttacat
    24301 gatgcaacgt tgtgaagaag ttgactgccc gaatcatggc aatgccgaag tcgctcatag
    24361 cgaatcacac tgatctatat cgatatactt atacttaggc tgctaacaaa attttgttgt
    24421 atgattgaaa ttagcggcct atactaattt cgagttgtta aagctacgat aaatattatg
    24481 tttttacggg gacaggatcg ttcccgactc actatggatg gtcatttcgg caagggttcc
    24541 tcctttccct ctgttctacg tcggattata gactcgcggt tttttctgcg agatttctca
    24601 caaagcccaa aaagcgtcta cgctgtttta aggttctgat caccgaccag tgatggagaa
    24661 actatgagtt catcgtgtat agaagaagtc agtgtaccgg atgacaactg gtaccgtatc
    24721 gccaacgaat tacttagccg tgccggtata gccattaacg gttctgcccc ggcggatatt
    24781 cgtgtgaaaa accccgattt ttttaaacgc gttctgcagg aaggctcttt ggggttaggc
    24841 gaaagttata tggatggctg gtgggaatgt gaccgactgg atatgttttt tagcaaagtc
    24901 ttacgcgcag gtctcgagaa ccaactcccc catcatttca aagacacgct gcgtattgcc
    24961 ggcgctcgtc tcttcaatct gcagagtaaa aaacgtgcct ggatagtcgg caaggagcat
    25021 tacgatttgg gtaatgactt gttcagccgc atgcttgatc ccttcatgca atattcctgc
    25081 gcttactgga aagatgccga taatctggaa tctgcccagc aggcgaagct caaaatgatt
    25141 tgtgaaaaat tgcagttaaa accagggatg cgcgtactgg atattggctg cggctggggc
    25201 ggactagcgc actacatggc atctaactat gacgtaagcg tggtcggcgt caccatttct
    25261 gccgaacagc aaaaaatggc tcaggaacgc tgtaaaggcc tggatgtcac cattttgctg
    25321 caagattatc gtgacctgaa cgaccagttt gatcgtattg tttctgtggg gatgttcgag
    25381 cacgtcggac cgaaaaatta cgatacctat tttgcggtgg tggatcgtaa tttgaaaccg
    25441 gaaggtatat tcctgctcca tactatcggt tcgaaaaaaa ccgatctgaa tgttgatccc
    25501 tggattaata aatatatttt tccgaacggt tgcctgccct ctgtacgcca gattgctcaa
    25561 tccagcgaac cccactttgt gatggaagac tggcataact tcggtgctga ttacgatact
    25621 acgttgatgg cgtggtatga acgattcctc gccgcatggc cagaaattgc ggataactac
    25681 agtgaacgct ttaaacggat gtttacctat tatctgaatg cctgcgcagg tgctttccgc
    25741 gcccgtgata ttcagctctg gcaggtggtg ttctcacgcg gtgttgaaaa cggccttcga
    25801 gtcgctcgct aaaggctatt ctatcgcccc ctcttcgggg gcgagttcag atcaggcttc
    25861 tgtacctggt tgattcatgg cattttctcg tgccgccagc acacgttcta ccgtatctac
    25921 cactgcctga gtttgtggat cgatttcaat gttgacgcgt gcgccaagtt ttttcttccc
    25981 aagagtcgtg cgttccagtg tttccggaat taaatggacg caaaaacgcg ttggcgtgac
    26041 ttcgccgacg gtcaggctaa taccgtcgat gccaataaat cctttgtaca ggatatattt
    26101 catcaactga ctatcctgga ctttaaacca gatctggcga ttattttccg aggttaatat
    26161 tttcgccact tcagcggtgg tcataatatg acctgacatt aagtgtccgc caatttcatc
    26221 actgaatttc gccgcacgct caacgtttac ccaatccccc acttttaaat cgccaagatt
    26281 ggtaatgcgt aacgtttctt tcatcaggtc aaaactgaca tggttgccgt taatttccgt
    26341 cacggtcagg cagcaaccgt tatgcgccac ggaagcaccg gtttccaggc cgtccagcat
    26401 gtggtcgggt aactccacca catgcgtacg aaaatttggt ttctcgtcaa tcgacaccag
    26461 ttttgcggtg ccctgtacaa tccccgtaaa catacttaca actcctgaaa tcagttaaga
    26521 cattctgttc agcacaatag caggtggaaa acgcccttac cagtgaaggg gtaagaatgg
    26581 ctattttttc actggagaat taataaatcc tcgctacaat agactgaatt tcccctgctt
    26641 cttctttttg ctgcccattc aggcggcttt ttagtctctc atataactac aaataaaagg
    26701 tgttcacgtg cagaagtata tcagtgaagc gcgtctgtta ttagcattag caatcccggt
    26761 gattctcgcg caaatcgccc aaactgcgat gggttttgtc gataccgtga tggcgggcgg
    26821 ctatagtgcc accgacatgg cggcggtcgc tatcggtact tctatctggc ttccggcgat
    26881 cctctttggt cacggactgc tgctggcatt aacgccggtt atcgcgcaat taaatggttc
    26941 cggtcgacgt gagcgcattg cgcatcaggt gcgacaaggt ttctggctgg caggttttgt
    27001 ttccgttctc attatgctgg tgctgtggaa tgcaggttac attatccgct acatggaaaa
    27061 catcgatccg gctctggcgg acaaagccgt gggttatctg cgtgcgttgt tgtggggcgc
    27121 gccgggatat ctgttcttcc aggttgcccg taaccaatgt gaaggtctgg caaaaaccaa
    27181 gccgggtatg gtaatgggct ttatcggcct gctggtgaac atcccggtga actatatctt
    27241 tatttatggt catttcggta tgcctgagct cggtggcgtt ggttgtggcg tggctactgc
    27301 ggcggtgtat tgggtcatgt tccttgccat ggtttcttac attaaacgcg cccgctccat
    27361 gcgcgatatt cgtaacgaaa aaggcaccgc taagcccgag cctgcggtta tgaaacgact
    27421 gattcaactc ggtttgccga ttgcgctggc actgttcttt gaagtgacac tgtttgccgt
    27481 cgtggctctg ttagtgtctc cgctcggtat tgttgatgtc gcaggacacc agattgccct
    27541 gaactttagt tcactgatgt ttgtgcttcc aatgtcgctg gcggcagcgg taactatccg
    27601 cgtaggttat cgtctgggtc agggctcaac gctggatgcg caaaccgctg cgcggaccgg
    27661 gcttatggtg ggtgtctgta tggcaaccct gacggccatt ttcacggttt cactgcggga
    27721 gcaaatcgcc ctgttgtaca acgacaatcc cgaggttgta acgctggctg cgcatttgat
    27781 gttgctggcg gcggtatatc agatttctga ctcaatccag gtgattggca gtgggatttt
    27841 gcgtggttat aaagatacgc gttccatttt ctatatcacc tttacagctt actgggtgct
    27901 gggcttgcca agcggctata ttctggcact gaccgatctg gtcgttgaac ctatggggcc
    27961 agcaggcttc tggataggct ttattatcgg cctgacatcg gcagccatta tgatgatgct
    28021 gcgtatgcgg ttcctgcaac gtatgccgtc agccatcatt ctgcaacgag cgtcccgcta
    28081 ataaagacaa ggcgcaacct tcacgggttg cgcctgtgtt tttacgcagg ctggagcgtt
    28141 gcgccaatcc cgtcttcgtc tggctgtaat ttcagagcgt tacacagagt ttgccgactg
    28201 gcatctgtta tctcaacgcg tatatccccg ttaacttccc ctttcacatt gatgaagtca
    28261 ggtgaaattt ctttcgcact ggtttgcagg caaatcgctc cctctccgcg caggcgtttc
    28321 actttcagtt gcacatgcgt tgccgcacca gtgatcagcc acagttttcc gccattcaga
    28381 ttgatgttac caacaatgaa agtgacgctg tcacctttta ccgtactgcc gtcgagaatc
    28441 aacacaccgc cctgctgtac agtaatcgtt gattgttgca gctctgcgcc ataaccaata
    28501 atcattgaag cgccgtccag taatgcaaca ctcccggtca gttcaaccgg tgcgcgatct
    28561 tcctctacga caaaattttc gtccgcgctg gtgggtgaca acaggatcac tgttccacct
    28621 tgtattaccg cattttccag cgtaccggca tgcacttcag cccgcccccc cacattcacc
    28681 gacaggtttt gcgtcttacc ggaactgtag agctgaaggc catccgtccc cagacgataa
    28741 atggatccat tctcaacaat agtgtcagag tcttcaccac cgagattatt catggtgcca
    28801 ccagcaagca acgtatcact ggctttcccg gccagcataa aaacgccgcc ctgattaatc
    28861 tcacaattgc tggcttctcc acttggatac accaacaatt caccaccttc atctaccacg
    28921 accgctgagg ttgtcccatt aaccaacagg ccgccctctt gatcgagaat gattttttcc
    28981 gcgcgatgtc cttccagtac acgcaggtta ccgccatttt ccagcaacaa accgcaggca
    29041 taaccctgat caacgctgaa ttcaccttcg ggatggcggc cattaaccgt agcgagcgta
    29101 gagagactaa ttgcgcctcc ctcggactga acaatatctg tggcaatggc accgtcgctg
    29161 accgatagcg tgccattgtt accgtactgt gtgcatttgg cgatcccacc ggttgccact
    29221 ttcaaccagc cagcccccgt gaccacggta ttttcggctc ttccgccagc ttcaacaatc
    29281 aactcgcctt cagtaagttg cgtcgcgcca aggacctcgc cggtcttaac aatttgcaca
    29341 ttcccgtcgc tcatcaagtt tttcgcatca gatcccatga tttattcctt tgctgcatct
    29401 gtgtgccttt attgctacct aagtgtaaag gctacggagg atttatccac gacagatttg
    29461 agatggtggc aaacaactct gtttaaactc tgatacacga attattgggt tgtatcagat
    29521 gtaaatgcga tcctgaataa aaatcgccct tgcaaatcaa caaaatatag ccaatttgct
    29581 taaaaactct gcaaacggtc gaaatagcgc agaaaattac gttttgcctc ttgccacctt
    29641 tccactctgc cgctaatatt cgtccccgtt gtcacctaca acgttgcgtt catagctcag
    29701 ttggttagag caccaccttg acatggtggg ggtcgttggt tcgagtccaa ttgaacgcac
    29761 catcctgcgt ccgtagctca gttggttaga gcaccacctt gacatggtgg gggtcggtgg
    29821 ttcgagtcca ctcggacgca ccagattttc ttaatctggt cttttccttt ttccctctgt
    29881 ttcttctctg tatccaatac gttaaaagat ttacactgtc ttcgtatgcg ttatcagaag
    29941 gagaatcgct atggcaactt tgttacaact tcattttgct tttaatggcc catttggcga
    30001 cgcaatggct gagcagctta aaccacttgc tgagtcgatt aatcaggaac ctggttttct
    30061 gtggaaggta tggacagaaa gtgaaaagaa ccacgaagcc ggtgggatct accttttcac
    30121 tgatgaaaaa agcgcccttg cctatctgga aaaacatact gcccgactga aaaatctcgg
    30181 cgttgaggaa gttgtcgcca aagttttcga tgtcaatgag ccacttagtc aaatcaatca
    30241 ggcaaaactc gcctgacaga atttaatcaa gggcggttag cgcccttttc atccctgtct
    30301 gaaatttctc aaattctaaa aatctcaacc aaacttatct gataacacta aattcgaaag
    30361 aatgcgtaca ggtaagtaac aatgaaaaaa attgctattg tgggtgccgg gcctacgggg
    30421 atctacacct tattctcgct tctacagcaa caaactccac tttctatttc tatcttcgag
    30481 caggctgacg aggccggtgt cgggatgcca tacagtgatg aggaaaactc aaaaatgatg
    30541 ctggcaaata ttgccagtat tgaaataccg ccgattaatt gtacgtatct cgaatggcta
    30601 caaaagcaag aagccagcca tctccagcgt tatggcgtta aaaaagaaac cttgcacgat
    30661 cgtcagtttt taccgcgaat tctgctgggc gaatatttcc gcgatcaatt tttacgatta
    30721 gtagaccagg cacgacagca aaaatttgca gtggctgttt atgaatcatg ccaggttacc
    30781 gatctgcaaa ttacaaatgc tggcgtcatg ctcgctacaa atcaggattt acccagcgag
    30841 acgtttgatt tagcggtgat tgccacgggt cacgtctggc ctgatgaaga agaagcaacc
    30901 agaacgtatt ttcccagccc gtggtcaggc ctgatggaag caaaggtcga tgcgtgtaac
    30961 gtgggtatta tgggaacatc cttgagcgga ctggatgcgg caatggcagt ggctattcag
    31021 catggttcgt tcattgaaga tgataaacaa cacgtcgttt ttcaccgcga taacgcaagt
    31081 gaaaagctaa atattacgtt gatgtcgcgc acgggtattt tacccgaagc cgatttctat
    31141 tgccctattc cctacgagcc cttacacatc gtcaccgatc aggcattaaa tgctgagatt
    31201 caaaaaggtg aagagggcct tttggatcgg gtatttagat tgatagtaga ggaaatcaag
    31261 tttgctgatc cagactggag ccaacgcata gccttagaga gcctgaatgt cgattccttt
    31321 gctcaagcct ggtttgccga gcgcaaacaa cgcgacccat ttgactgggc agaaaaaaat
    31381 ctccaggaag tcgaacgcaa taaacgagaa aaacatactg ttccctggcg ttatgtcatt
    31441 ctgcgcctgc atgaagccgt acaggaaatt gttcctcatc tgaatgaaca cgaccataaa
    31501 cggttcagta aaggccttgc cagggttttc atcgataatt atgcggcaat cccttcagag
    31561 tctattcgtc gactactggc cttacgtgaa gcggggatca ttcatattct cgccctcggt
    31621 gaagactacg aaatggaaat taatgagtcg cgcaccgtcc tgaaaacgga agacaacagc
    31681 tactcgtttg acgtttttat tgatgcccgc ggacagcgtc cgcttaaagt gaaagatatt
    31741 cctttccctg ggctacgcga gcaattacag aaaacagggg atgaaatccc tgatgttggc
    31801 gaagattata cgttacagca acccgaagat attcgtggac gcgtagcgtt cggcgcgttg
    31861 ccctggttga tgcacgacca gcctttcgtt cagggactta cggcatgtgc agaaattggt
    31921 gaggcgatgg ctcgggcggt cgtaaagcct gcatcccgtg ctcgtcggcg tctttcgttt
    31981 gattaaaggt aaagctatca gcactcaaca aactgatgct gttcattaag acgatagcgg
    32041 actccggttc gaatattgtt ttcaccttca atggcgacgg cgaagcgaac tctttcgccg
    32101 tcatgatatg ccagtgcggc gcttccccct agcccgagaa taattgagtc gaccacgccc
    32161 gtgctcacta ccgttgaatt ttcaccgctg gcaatgatgt gtacattgtc gccgctgttg
    32221 gcaattctgg cgtttgcgcc aaagctggcg atttgcacca ggtcaccatt actggcgaca
    32281 tgacaccgtt cacccaatgt acaaacgcgt actcgcattc cggtattcgc aatccgacta
    32341 ctatctccag cgctgcttat ccgcgaagaa ttaccggtta cagcaacccg tgcgcgttca
    32401 ccggagctgc caatgtgact gttaaagccg acacttccaa tccgcacgct ataacccata
    32461 ctggcaattt gcgcggcata cccagcggtt gcgattcggg cgttatcctc acaacagccg
    32521 atgcgagcat gatcgtcgct gcgggcaaac tgactgcaaa atggtatctc tccggggtct
    32581 gtggccagtt tttgcataga ggaaacttcc tgatgcgcga agttttcatc cgccagccat
    32641 tttttccacg catactcaac cagactctcc gcccagtccg tatatccttc ctcaatcagt
    32701 gcatgacgaa tatcggcata actaccgccg cgtgggaaat ggcgcaggaa ccagcgatac
    32761 atcaccgctc cgatacgcca ttctcgcagg tctgctcgtg taatgatcat tcgttagtgc
    32821 cggtgatagc catcgaccat gcttttaaag gtttcgtgtg gcgtacgccc cgtggtgcaa
    32881 agataaagat gaccgaagat aaaaaagagg cttataaatg ccagagcaaa atgcgcctgc
    32941 aataaccagt atcttacgcc aggaaacaca tctcccacgg cttgcggata gagacacaac
    33001 agccccgtca atagtaacaa cggcagcaat ccatacatga caccaacata ggcgacctgc
    33061 tgtaagggat taaatttaga ctgtgttgtt gccgggaaag gatgttcttc cccctgcata
    33121 atgccaaaca aataaaatcg cgtttgtttt gccgctcgtt ccagccaccc ctgacgacga
    33181 atgcgatagt ggtgaccatt atccccaacg gcattgatca gcacaaagcc gagccagcat
    33241 gccagtaaca aaaatccgca aacttcatgc accgcaacca gacttttaac cgcagttgcg
    33301 cccaccatcg caaagtgatt tatcagccca cttgccagca acaatacaaa gagtaacgca
    33361 ttcgaccagt gccataatct gaccgctttg ctgtaaagat aaaccttctc accatgatcg
    33421 gtggctgatt tcttcacgcc acgagcacga agcaaggcgt gcagccccag caccaaccac
    33481 atcccaacca gcaatactcc ggcaatgatc aaccacaccg gccaaaattc gggagtgaac
    33541 tgtggcacat agttcgctaa ctggctctga aactgttcag catgttgcga cgggttcata
    33601 cattttcctt tttaatcaaa tgttgaccga accggcgata cagatgcggt tttccggcac
    33661 caggtagctg atattgatag tatttattat cctgcaacca cgcctgaatt tctgggctat
    33721 cttcgcgacc aaagatcaat gcgtgttccg gacaggcgct gacgcaaatg ggcggaaagc
    33781 cttttgccag tcgggactca gcgcagaaat cacatttatc tgccacttta gtgacagggt
    33841 taaggtagcg aacctggtac ggacaagcgc caatgcaata actgcagccg atgcactgcg
    33901 atttttctac ccgcacgatc ccctgctcat cacgccacga tgcccccgtc gggcatacgt
    33961 cgatgcacgg agcatcttcg cagtgctgac atgactggcg aaaaaagtga tactgcgttt
    34021 cgttgtcgtt atcggtgact ggaatgtgtg ctatcgataa acggcttccc tgagcgggga
    34081 cgtggttagt tttacggcat gcgcgggcgc aaatattaca gccattgcac cgtgactcgt
    34141 catgaatcat ggcataccga acttcctttt cttgcctcgt gttcgctaac agagaagacg
    34201 cagaaccagt aaaaaagatt actgttccca tccccagaac aaatttgcgt cgagtaaagg
    34261 acatctgatt atcccctaat gcagtggctg caactgtcca ccctatccat gacatctccc
    34321 tgaaatttca ggggttctga aactgctcta cctcgaacca ttcgcataag ccataagcct
    34381 tgccataggt cataaatcgc tcgctaaaca agtacaacac cgccgtcgat gactccacct
    34441 gccgtatatc ggcatattca ggtgcaacat ccatcgcggc gatcgcggct tcaatctgcg
    34501 cttcctggaa gtaatatggc gcttgcatca gcatggcgac tttgtaagga cgaggatacg
    34561 tctggcactc aaaacgaacg gcgtgtgcaa tggcgcgaca aatgtcctgc tccacaactt
    34621 gcaaagacat cgccgcgtag ttttcactca tggcctgcgt tgagtaatag taatcatcct
    34681 gacttcccgt catacaagcg atatccgcgt agtctccgtt ctgcttaatg ctttccagca
    34741 aaactgacag ttcctcttct gcaaccgaat agggtggcga gagaaacacg gtgcgcgcca
    34801 ccagctgccc agccgcagaa tgctggcgaa tgaactcagc caggatttca gcaggttctg
    34861 gttcggttac cgtatcaacg gggtgacgca tcccctgcaa ccattgccgc ttcgcctcgt
    34921 caacttcgct gactttcgct aacggcaatt catcctgatg gttcatctta cccattcctt
    34981 acgcaggcag tagattgtgt gctgccagat cggcggcaat atcttccagc cccagacgct
    35041 gcaatgtttc gcgggttgga caaccaagct gtgggtccca gcccatttct ttgtagaaca
    35101 tcgtgagcga agcatgcata tcgtcacgat ccattttgtc agtaccttca gtaaagaccg
    35161 ggatctgcgg atccttgtcg aaaacccagg aacagataag atcgtgttca ttacgcatat
    35221 ctttggtttg catcggtttt accgtgtagg cacgatgcaa cgtaaagata cgctctgcag
    35281 ctaaatctaa tttttcctga gtcatctctt cgccggtgat cgctttgaag aatttggctt
    35341 aaagcgccag atcgccccgg taattacggc ttttcagcgg cgaaacggtc attggccaga
    35401 cccagttgca cagagtgacg gcgttatgca aacagacccg caacagcgac cactttgcat
    35461 atttaatttt tgcgtcattg attggcgtgt agtttttggt ttcatcgtaa gcatcttcag
    35521 aaccaaaaag ttctttcgcc acttcacgtt gcagtttcaa tggcaagccg gaaccaataa
    35581 agttgatatg ggtatgcgtc atgcagtcac ggttgaacat acagttaaca atggaaccga
    35641 cctgcgctga cgcttcattg gcatggtgaa ccggatagcc aaacggcgac cagagtttat
    35701 ttttcgcgta gccccagtac tctttaccca aattccagcg ttctgcgatg gcatatgaac
    35761 catcagccag gtgactcagc tcacccacac gatgcgccag acggtagtaa aaatctttaa
    35821 tgaagttaac gtcacccgct tccagttgat cccagcgaat ttctgcatac tcttcagctg
    35881 gcagaacacg cttgaacaca cctttgctgt aacaataagt aaagtcgcga tgcaactgcc
    35941 cgtagttaca ccataggccg tagtcatcga acaaattcag accaaccagg ttaccaatca
    36001 cacgaccatc gtctttatct tcaaaatctt tcggcccgtt cgggaagatg gtggtatgga
    36061 caaagtttgc tacacaagtg ttaccacctg tgctgggcac gccaaactct ttcacccgag
    36121 gaatattcat ttgggtcata caacggatcg ggcaagagtg gcagccgctc atttttactg
    36181 tgtatttctc tgccgccggt cctaagtcaa aaacggattt ataggtacga aagccgaccg
    36241 tattctgatt gcctggcgga atttcacccg tttcaatcgg accaccttca gccgcgcccc
    36301 aaaacagccc tttacgtgct gtccagcgtg acttgggatc tgaatactct gcccacgatt
    36361 gtggcgtact tggcacgaca tggttgttat tcgcaccaat aagttcagtc atcatgtaat
    36421 cattgagacg cttcatctcc tgacgatcgg caatgttgac ccctttcgtc ccttcaaccg
    36481 caatcgcttt caggtttttc gaacccatta ttgcgccagt tcccgcaccg ccgctgtggt
    36541 tacggctatt caacatgcca gagagaggaa taaggttttc cccagcctga ccaatagccg
    36601 ccacacaggt ttccgggctg gtcaatcgac aaatttcttc cgtcgttgcg cgcgtccctt
    36661 ttccccataa gaaatcggct ttttccaggc taactttgtc atctttaatc ttcagccata
    36721 ccggtgattt cgccttccct tcgataataa tgacgtcgta tccagcgaat ttcatttgcg
    36781 ctgcaaaaaa gccacccata tgggcatcgc gactaaattt cctttggtaa aagtagaaag
    36841 tgaggtgata tttacgcgag aactacaggg ggcaccagat ccagttaatg ggccggtagc
    36901 aaagactaat ttattcgctt catcgaaagg tttcgtgcct agcggtactt cgtcatacat
    36961 aattttgtag ccgaagccca tgccaccgac aaaactttta aacttactgg aatcttcgag
    37021 ggtaatattt cctgtcgtga gattgactct taatatatta cctgtccaac cgttagccat
    37081 gattttttcc tttgcaagat tcacacagta atatctttcc actcgataat ttttaacgcc
    37141 cctgttgggc aggcgtttgc gcattcaccg cataacacac atttcgagga ttttttactt
    37201 tcggtattta cggtggccat catccacggg catgccgtag tacaggcgct acagccaata
    37261 caacgtttat gatcgacggt aatacagcct tctttctgct gccaggtaat cgcaccaatc
    37321 gggcagacgt tcatgcattg cggctctttg cattgacggc aggtgtccgc ggtatagttg
    37381 agatcgccat acaggccgcc gccagagcca accccgttgt cgccaaagaa ataattgcga
    37441 tggattttga tacgggagaa gaatgtccct actgagccat cgttgaagtt ggtacaagag
    37501 atttcacagc ggtggcaacc cgtgcatcgc gctcgctggg tcaccagcac ccctttgggt
    37561 gtatttatca accctactgt gccgctatca atatcttcct gtttgcaacc caaaagtgac
    37621 agtaacgcag gggcaattgt tagaccagca aggccttttc ctgatatacg caagaattct
    37681 aatcgcgtta agccaatatc taatagtgga cgatcaaccg ggttcattta tttcgcatcc
    37741 tcttcgcaaa cacagtgctg acaattcagc agcctgatat taagcattca actaatacgc
    37801 tgctgactct gtccccaaca ccagatattt tccctaaccc gatagtgtat gagaaaagat
    37861 ttaacgccga taaactaatc ctttatggtt aggagaaaaa ataatgtgat tattctgagc
    37921 ccttaacatt gatcgttatc aattaaaatt acaaagcaag aaattataag cgaactgata
    37981 tttattatca tttgaaataa atttaactta agcgacaatc atttgtgacg ttcattgcag
    38041 aaaataaaaa taacattatc gctataaatt aaaatacata ccgaaattcg tataatttca
    38101 gcagttgtta tttagaatga ttaactggac cgcaactgaa gcaagaatat caggatgaag
    38161 cttaccgcct catcctgaat ggtcatttta ccagtgcgtt aatgacggtt tccatcgcct
    38221 gcttttccag ctcactgcgc tgaatgcgtt tattggctaa tgctgtacgt aatacgcctg
    38281 cgataacaat atgctttggt tcctgcccca gatcgcgcat ttccagcaca actttaccga
    38341 caactctgca catttctcgg tacagttcat cctcttttgt ccgatttccc attctttata
    38401 cctatttatc atgccaacta tcaggtaatg ctgccaactt actgatttag tgtatgatgg
    38461 tgattttaag gtgcttgcgt ggcttccatt tccatcagat gtccttcctg ctccgctact
    38521 gaaggcgtgg t
//
LOCUS       DAEEGA010000032        37876 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEEGA010000032 DAEEGA010000000
VERSION     DAEEGA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37876
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.032"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..176)
                     /locus_tag="KLH46_001765"
     CDS             complement(<1..176)
                     /locus_tag="KLH46_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBE0378472.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="KLH46_001766"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="KLH46_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBE0378473.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="KLH46_001767"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="KLH46_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBE0378474.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="KLH46_001768"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="KLH46_001768"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBE0378475.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="KLH46_001769"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="KLH46_001769"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBE0378476.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="KLH46_001770"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="KLH46_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBE0378477.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="KLH46_001771"
     CDS             complement(5180..5422)
                     /locus_tag="KLH46_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBE0378478.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="KLH46_001772"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="KLH46_001772"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBE0378479.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGKRCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="KLH46_001773"
                     /gene_synonym="fabE"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="KLH46_001773"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBE0378480.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="KLH46_001774"
     CDS             complement(8339..9313)
                     /locus_tag="KLH46_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBE0378481.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="KLH46_001775"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="KLH46_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBE0378482.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="KLH46_001776"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="KLH46_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBE0378483.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="KLH46_001777"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="KLH46_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBE0378484.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="KLH46_001778"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="KLH46_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBE0378485.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="KLH46_001779"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="KLH46_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBE0378486.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="KLH46_001780"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="KLH46_001780"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBE0378487.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="KLH46_001781"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="KLH46_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBE0378488.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAAGKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="KLH46_001782"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="KLH46_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBE0378489.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="KLH46_001783"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="KLH46_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBE0378490.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /locus_tag="KLH46_001784"
     CDS             23180..23383
                     /locus_tag="KLH46_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBE0378491.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="KLH46_001785"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="KLH46_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBE0378492.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="KLH46_001786"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="KLH46_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBE0378493.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="KLH46_001787"
     CDS             26388..26660
                     /locus_tag="KLH46_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBE0378494.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="KLH46_001788"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="KLH46_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBE0378495.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="KLH46_001789"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="KLH46_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBE0378496.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="KLH46_001790"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="KLH46_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBE0378497.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="KLH46_001791"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="KLH46_001791"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBE0378498.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31741)
                     /gene="degQ"
                     /locus_tag="KLH46_001792"
                     /pseudo
     CDS             complement(30406..31741)
                     /gene="degQ"
                     /locus_tag="KLH46_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31895..32293)
                     /locus_tag="KLH46_001793"
     CDS             complement(31895..32293)
                     /locus_tag="KLH46_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBE0378499.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32487..33614
                     /gene="zapE"
                     /locus_tag="KLH46_001794"
     CDS             32487..33614
                     /gene="zapE"
                     /locus_tag="KLH46_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBE0378500.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33834..34262
                     /gene="rplM"
                     /locus_tag="KLH46_001795"
     CDS             33834..34262
                     /gene="rplM"
                     /locus_tag="KLH46_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBE0378501.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34278..34670
                     /gene="rpsI"
                     /locus_tag="KLH46_001796"
     CDS             34278..34670
                     /gene="rpsI"
                     /locus_tag="KLH46_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBE0378502.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35065..35703
                     /gene="sspA"
                     /locus_tag="KLH46_001797"
     CDS             35065..35703
                     /gene="sspA"
                     /locus_tag="KLH46_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBE0378503.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35709..36206
                     /gene="sspB"
                     /locus_tag="KLH46_001798"
     CDS             35709..36206
                     /gene="sspB"
                     /locus_tag="KLH46_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBE0378504.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36249..37616)
                     /gene="dcuC"
                     /locus_tag="KLH46_001799"
     CDS             complement(36249..37616)
                     /gene="dcuC"
                     /locus_tag="KLH46_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBE0378505.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(37637..>37876)
                     /locus_tag="KLH46_001800"
     CDS             complement(37637..>37876)
                     /locus_tag="KLH46_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0378506.1"
                     /translation="LLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNL
                     RQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         9175 a         9483 c         9951 g         9267 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatacc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaaccggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatccg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagcgt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgcgcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgtttg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgcag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctcc ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gtctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 cactgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 caggcaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacttggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ccggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag ggcaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctaggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttc gctgagccag
    30841 aacttggcaa cacttcgctg acaaacgcgc cacgctgcac gtcaaggttg aaggctttgg
    30901 cgatatcggc actcatctcg gtgcctttga tgcctaacaa accgcgtttg atttcaccaa
    30961 agtcgataag ctgctgcgcc agtgttcgcg ccatattact ggggatggca aatccaatcc
    31021 cgacgctccc gccgccaggc gcaaggattg cagtgttgat gccaattaac tcaccgttaa
    31081 ggtttaatag tgcaccgccg gagttaccgc ggttaatgga agcatctgtc tggataaagt
    31141 tttccagacc ttcaagattc aacccgctgc ggcctaatgc ggaaacaatg ccagaggtgg
    31201 cggtttgccc aaggccaaat gggttaccga ccgctacggc aaaatcaccg acgcgcaatt
    31261 tatcggagtc ggcaatagcg atttgcgtta atttgctcgg gttttgaatt tgtaacaggg
    31321 cgatatcgct ctggtcatcg ctaccaatca gttttgcatc aaactcgcgc ccatcattga
    31381 gctgaatact gattttctgt gcctgattaa tcacatggtt gttggtcagc acatagcctt
    31441 tactggcgtt gatgatgaca ccggaaccta aaccttcgaa gggttgtgca ggttgatccg
    31501 gtaaatcatc accaaaaaac tttttgaatt cttccgggat tttctgtccc tgactggccg
    31561 ttccttccac ccgtacgctc accactgccg gaagcacttt ttccagcatt ggagccagac
    31621 tggggagagg ggcctgatcg gcaacctggc ctggaatcga cgcgacggcc tgaaatgacg
    31681 ccgagagagt taacccggca cttaacgcta atgcactcaa cagctgggtt tgttttttca
    31741 ttattcctgc tctcgtacct gaatgataag aaaagagatt caaaacagtt tgatgttatt
    31801 gaattttcag acggctaaca atgagacacc agattaaatg atagtcgggg agggagagaa
    31861 gaggagggcg caatggctgc gcccgaaaaa taaattagtc gcgcttcgcg ccagtacgca
    31921 gcaggccgga tgcgccttca gaatagtcgc gaggcacctg caccggtgcc tgatcgttgc
    31981 tggcttcaga ctctgccaga cgattacgga acgggtttgc ttcagcagac agttccggca
    32041 gcaggctgct ggagcttttt gccatgtgct gatacagctg gcgatagtcg tgcgccatgg
    32101 tatccagtaa ttccgcgctg cgggcaaagt ggctaaccag ctcttcgcga tactcgtcca
    32161 gttcagcttt attcttttcc agttcgtact gcaacgcctg ttgctggcgt agtttacgat
    32221 taccaaaacg catggccaca gcaccaataa tgatgccgac gactaaccca attagcgcat
    32281 attcccaggt catgaacatc tcccgttgtc ttttgtttcc gtagggtgtt ggcttcaggc
    32341 tccatgcctg cggctgatta tgccactata accgttaatt ccacagaagt ggaatcccga
    32401 ctgcatatcg cgtagtgtag aacggccttt ttttcgtcaa ccgtgaacaa cagcgcaccg
    32461 attattcaag gaataacaat aagatcatgc aaagcgttac cccaacatcg caatacctga
    32521 aggcgctcaa tgaaggcagc catcaacccg acgacgttca aaaagaggcc gtcagccgcc
    32581 tggaaattat ttatcaagaa ctcatcaata gcaggccacc agcccccagg acgagtgggc
    32641 taatggcgcg ggtcggtaag ctgtggggta aacgcgaaga cacaaagcat acgccagtgc
    32701 gtggcttata tatgtggggc ggtgtaggac gcgggaaaac ctggctgatg gaccttttct
    32761 atcaaagcct gccgggagag cggaaacagc gcctgcactt tcaccgtttt atgctgcggg
    32821 tgcacgaaga gctaactgcc ttacaggggc agaccgatcc gctggaaatt attgccgatc
    32881 gctttaaagc cgaaactgac gtgctctgtt ttgacgaatt ttttgtttct gatattaccg
    32941 acgccatgct acttggcggt ctgatgaaag ccctgttcac ccgcggtatt accctggtag
    33001 cgacgtcaaa tattccgccg gacgaacttt atcgaaatgg cctgcaacgt gcgcgttttc
    33061 tgcctgcaat cgatgccatt aaacagcatt gtgatgtaat gaacgtggac gctggtgttg
    33121 attatcgact gcgtacactc actcaggcgc atctgtggct ttcgccactc aacgatgaaa
    33181 cccgggcgca gatggataaa ctatggttgg cgctggcggg ggtgaaacga gaaaattcac
    33241 cgacgttaga aatcaaccat cggccattgg cgacaatggg cgtcgagaac cagacgctgg
    33301 cggtttcttt tactacgctg tgcgtcgacg cccgcagtca gcatgactat attgcgctct
    33361 cacgcctgtt tcacacagta atgttgtttg atgtgccagt catgacgcgg ttgatggaga
    33421 gcgaagcgcg gcgctttatt gcgctggtgg atgagtttta cgagcgccat gtcaaattag
    33481 tggtgagtgc agaagtgccg ctgtatgaaa tttatcaggg tgagcggctg aagtttgagt
    33541 tccagcgttg cctgtcacgt ctgcaagaga tgcaaagcga agagtatctg aagcgcgagc
    33601 atttggcggg ttaaaacctg ccacaaatca caaaaagggg tcgatctttg accccgactt
    33661 ctctataatc ctgcgacccc acgttacaag aaagtttttt tccccaaaac tttttgtgtg
    33721 ctggcatagg ctattcgaag gggtaggttt gccggacttt gtcgtgtgaa cctcaacaat
    33781 tgaagacgtt tgggtgttca ccaacgtgta actatttatt gggtaagctt ttaatgaaaa
    33841 cttttacagc taaaccagaa accgtaaaac gcgactggta tgttgttgac gcgaccggta
    33901 aaactctggg ccgtctggct actgaactgg ctcgtcgcct gcgcggtaag cacaaagcgg
    33961 aatatactcc gcacgtagat accggtgatt acatcatcgt tctgaacgct gacaaagttg
    34021 ctgtaaccgg caacaagcgt actgacaaag tgtactatca ccacaccggc cacatcggtg
    34081 gtatcaaaca agcgaccttt gaagagatga ttgctcgccg tcctgagcgt gtgattgaaa
    34141 tcgcggttaa aggcatgttg ccaaaaggcc cgctgggtcg tgctatgttc cgtaaactga
    34201 aagtttacgc gggtaacgag cacaaccacg cggcacagca accgcaagtt cttgacatct
    34261 aatcgggatt ataggcaatg gctgaaaatc aatactacgg cactggtcgc cgcaaaagtt
    34321 ccgcagctcg cgttttcatc aaaccgggca acggtaaaat cgtaatcaac caacgttctc
    34381 tggaacagta cttcggtcgt gaaactgccc gcatggtagt tcgtcagccg ctggaactgg
    34441 tcgacatggt tgagaaactg gacctgtaca tcaccgttaa aggtggtggt atctctggtc
    34501 aggctggtgc gatccgtcac ggtatcaccc gcgctctgat ggaatacgac gagtccctgc
    34561 gttctgaact gcgtaaagct ggcttcgtta ctcgtgacgc tcgtcaggtt gaacgtaaga
    34621 aagtcggtct gcgtaaagca cgtcgtcgtc cgcagttctc caaacgttaa ttggcttctg
    34681 ctacggcaga aaacaatttg cgaaaaaacc cgcttcggcg ggttttttta tggctaaatt
    34741 ctgaatcagc gtaaaaaatg ggaagttgtt ttttgctgcc gtctgaaaga caggtaaaac
    34801 aaaaaccaca tcgccaataa gggtctaagt caactatttc agactaaagc gcatctcttt
    34861 ttccccattt ccggcatcca ctcaccacaa aggtcgcaaa atctggtaaa ctatcatcca
    34921 attttctgcc caaatgtcgg gtattgctca ttttttgttt gattttcgaa caaagagagt
    34981 cgttccttat tgggtaacac aacttctgac tggccacctg gtggctggta gcagtaaaaa
    35041 ttctgactat acctggaggt tttcatggct gtcgctgcca acaaacgttc ggtaatgacg
    35101 ctgttttccg gtcctactga catctatagc catcaggtcc gcattgtgct ggctgagaaa
    35161 ggtgtaagtt tcgagatcga acacgtggaa aaggacaatc cgcctcagga tctgattgac
    35221 ctcaacccga atcagagcgt tccgaccctg gtggatcgtg agctgaccct gtgggaatct
    35281 cgcatcatta tggaatatct ggatgagcgt ttcccgcatc cgccactgat gcctgtttac
    35341 ccggtagctc gcggtgaaag ccgtctgtac atgcatcgca tcgaaaaaga ctggtacacg
    35401 ctgatgaaca ctatcatcaa cggttcagct tctgaagcag atgccgcacg taagcaactg
    35461 cgcgaagaac tgctggcgat tgcgccggtc ttcggtcaga agccgtactt cctgagcgat
    35521 gagttcagcc tggtcgattg ctaccttgct ccgctgctgt ggcgtctgcc gcaactgggc
    35581 atcgagttca gcggcccggg tgcgaaagag ctgaaaggct atatgacccg cgtctttgag
    35641 cgtgactctt tccttgcttc tttaactgaa gcagaacgtg aaatgcgtct gggccggagt
    35701 taatctgtat ggatttgtca cagctaacac cacgtcgtcc ctatctgctg cgtgcattct
    35761 atgagtggtt gctggataac cagctcacgc cgcacctggt ggtggatgtg acgctccctg
    35821 gcgtgcaggt tcctatggaa tatgcgcgtg acgggcaaat cgtactcaac attgcgccgc
    35881 gtgctgtcgg caatctggaa ctggcgaatg atgaggtgcg ctttaacgcg cgctttggtg
    35941 gcattccgcg tcaggtttct gtgccgctgg ctgccgtgct ggctatctac gcccgtgaaa
    36001 atggcgcagg cacgatgttt gagcctgaag ctgcctacga tgaagatacc agcatcatga
    36061 atgatgaaga ggcatcggca gacaacgaaa ccgttatgtc ggttattgat ggcgacaagc
    36121 cagatcacga tgatgacact catcctgacg atgaacctcc gcagccacca cgcggtggtc
    36181 gaccggcatt acgcgttgtg aagtaataca aaacaggccc aggcggcctg ttttgtcttt
    36241 ttaatgactt aataaaatag cgtgattgtg gcaatcatat tcaccacgaa accgactgcc
    36301 atggggatcg ctgtccgctt caccaccgca aaaggcgaaa cgcctgcaat tcctgacgta
    36361 acgaccacca ccgcagtaat cggcgaaacc gcgcgcgcta gcgtcgtggc aaaatgcatc
    36421 ggcataatca ttacaaccgc tggtacatgt agtccggctg cgatattcgg aataagactg
    36481 gcaaatgaca taaacggcgc attgccagag cccatcacaa tggcacaaat ggcaatgact
    36541 agcgccataa taatcatcac gccaataccg cccagaccag aatgctccgc acccctgata
    36601 accgcatcaa cagtgccaat cgtcgttaag cctttcgcaa atatttcacc cgcgaccacc
    36661 agcgttacca cgttgacaaa ctgcgtaccc atgccgtcaa aaaacgcctg cacatcgtcc
    36721 attgtttcgc gcaagttatg cttgcggaag aactcaacaa acatcgtcac cgtcaaactt
    36781 agtaacatca ccaccaccag atgcagttct gacttcatca gcccgacgtg ggcgaggaac
    36841 agcgagccga gcatcaggat taacggcatc acaggtaaaa tggcgtaata gagcggcggg
    36901 acattatcga gagttttttg ctctgcctgt tcgtgattga tatttttatc ttttttgtca
    36961 aaagcgcgtt gcacgaaaaa gtgggagatc gccaccgaga taatgacgca agaggcgacc
    37021 ggaagctggt agtggaagaa gtatgtggca attttcattc ccgcgacctg ggctgcaaaa
    37081 atggagttcg tttccagaat cccccactca atggacatcg tggttgcgat aaccgccacc
    37141 gcagagaggc gacttactcc cagactcacc agcgtcggaa ataaggtgac catcagcaac
    37201 atacccagac cggaggcgct ggtaataaac tgcgccatga tttggccgat gacgtaagtt
    37261 gccgacagaa taatatacgg cgagcgaatg agttttaacg ggcggcttag caagctcacc
    37321 atcgcgcaac tggctcctat gcgctccatg tagcgggcat aaccgcccac cgccataatc
    37381 gacagcccca atccggcaat gcggttggac agcatgcgca ggatttcatt ataaatgtcg
    37441 acgatcaagt agccgctgct tttggcggga tcgagtacac caccgaaccc taaccagact
    37501 ccgcacatca tcaggaagac acctgcggca gccagcacca cctgaggttt gtagtttttc
    37561 aggatcaaat agcccatcgt aattaatacg atgacagata taattatgcc gaacattgtt
    37621 gggtaatgac tccaacttac tgatagtgtt ttatgttcag ataatgcccg atgaccttgt
    37681 catgcagctc caccgatttt gagaacgaca gtgacttccg tcccagcctt gccagatgtt
    37741 gtctcagatt cagattatgt cgctcaatgc gctgagtata acgcttgctg ataacgtgca
    37801 gctttccctt caggcgtgat tcatacagcg gccagccatc cgtcatccat accacgacct
    37861 caaaggccga cagcag
//
LOCUS       DAEEGA010000033        36889 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEEGA010000033 DAEEGA010000000
VERSION     DAEEGA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36889
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.033"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..247
                     /locus_tag="KLH46_001801"
     CDS             <1..247
                     /locus_tag="KLH46_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBE0378507.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="KLH46_001802"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="KLH46_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBE0378508.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="KLH46_001803"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="KLH46_001803"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBE0378509.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="KLH46_001804"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="KLH46_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBE0378510.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="KLH46_001805"
     CDS             complement(3258..3542)
                     /locus_tag="KLH46_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378511.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="KLH46_001806"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="KLH46_001806"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBE0378512.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="KLH46_001807"
     CDS             complement(4443..5651)
                     /locus_tag="KLH46_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0378513.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="KLH46_001808"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="KLH46_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBE0378514.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="KLH46_001809"
     CDS             complement(6446..8131)
                     /locus_tag="KLH46_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBE0378515.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="KLH46_001810"
     CDS             8401..8778
                     /locus_tag="KLH46_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBE0378516.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="KLH46_001811"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="KLH46_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBE0378517.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="KLH46_001812"
     CDS             9225..9512
                     /locus_tag="KLH46_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBE0378518.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="KLH46_001813"
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="KLH46_001813"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBE0378519.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="KLH46_001814"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="KLH46_001814"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBE0378520.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="KLH46_001815"
     CDS             11269..11745
                     /locus_tag="KLH46_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBE0378521.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="KLH46_001816"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="KLH46_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBE0378522.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14437
                     /gene="potG"
                     /locus_tag="KLH46_001817"
     CDS             13304..14437
                     /gene="potG"
                     /locus_tag="KLH46_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBE0378523.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="KLH46_001818"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="KLH46_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBE0378524.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="KLH46_001819"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="KLH46_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBE0378525.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="KLH46_001820"
     CDS             16302..16790
                     /locus_tag="KLH46_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBE0378526.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="KLH46_001821"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="KLH46_001821"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBE0378527.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="KLH46_001822"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="KLH46_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBE0378528.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="KLH46_001823"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="KLH46_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBE0378529.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="KLH46_001824"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="KLH46_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBE0378530.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="KLH46_001825"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="KLH46_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0378531.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="KLH46_001826"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="KLH46_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBE0378532.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="KLH46_001827"
     CDS             complement(22242..22757)
                     /locus_tag="KLH46_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE0378533.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="KLH46_001828"
     CDS             23406..25175
                     /locus_tag="KLH46_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBE0378534.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="KLH46_001829"
     CDS             25386..25709
                     /locus_tag="KLH46_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBE0378535.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="KLH46_001830"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="KLH46_001830"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBE0378536.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="KLH46_001831"
     CDS             complement(26533..27546)
                     /locus_tag="KLH46_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBE0378537.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="KLH46_001832"
     CDS             complement(27645..29075)
                     /locus_tag="KLH46_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBE0378538.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="KLH46_001833"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="KLH46_001833"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBE0378539.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="KLH46_001834"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="KLH46_001834"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBE0378540.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="KLH46_001835"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="KLH46_001835"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBE0378541.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="KLH46_001836"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="KLH46_001836"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBE0378542.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="KLH46_001837"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="KLH46_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBE0378543.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="KLH46_001838"
     CDS             complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="KLH46_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBE0378544.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9231 a         9166 c         9452 g         9040 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
     2341 aagcttctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc
     3721 tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc acgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg cgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggcaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgctacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
    11341 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgctgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 ctagaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gtgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg attcgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcttggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggaaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaattggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg gggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tataaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tgggtgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatactttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata attgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcct gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat tgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccagct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgctagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcat cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaata caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32401 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgtat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gttcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 ttttctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggg taagacgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gaactaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaatagct tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aagggcgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg
    35761 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
    35821 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
    35881 cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
    35941 cgggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
    36001 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
    36061 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
    36121 ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    36181 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    36241 ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca
    36301 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
    36361 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
    36421 cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
    36481 ccgttttacc actggcaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
    36541 gggcaattac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
    36601 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
    36661 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
    36721 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
    36781 cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt gaaaaatagg
    36841 taatgactcc aacttactga tagtgtttta tgttcagata atgcccgat
//
LOCUS       DAEEGA010000034        36520 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEEGA010000034 DAEEGA010000000
VERSION     DAEEGA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36520
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.034"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(156..1391)
                     /locus_tag="KLH46_001839"
     CDS             complement(156..1391)
                     /locus_tag="KLH46_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBE0378545.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="KLH46_001840"
     tRNA            complement(1538..1614)
                     /locus_tag="KLH46_001840"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="KLH46_001841"
     tRNA            complement(1657..1743)
                     /locus_tag="KLH46_001841"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="KLH46_001842"
     tRNA            complement(1764..1839)
                     /locus_tag="KLH46_001842"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="KLH46_001843"
     tRNA            complement(1898..1974)
                     /locus_tag="KLH46_001843"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="KLH46_001844"
     CDS             complement(2077..3462)
                     /locus_tag="KLH46_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE0378546.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="KLH46_001845"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="KLH46_001845"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBE0378547.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="KLH46_001846"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="KLH46_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBE0378548.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="KLH46_001847"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="KLH46_001847"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBE0378549.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="KLH46_001848"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="KLH46_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBE0378550.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="KLH46_001849"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="KLH46_001849"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBE0378551.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="KLH46_001850"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="KLH46_001850"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBE0378552.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="KLH46_001851"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="KLH46_001851"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBE0378553.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="KLH46_001852"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="KLH46_001852"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBE0378554.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="KLH46_001853"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="KLH46_001853"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBE0378555.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="KLH46_001854"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="KLH46_001854"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBE0378556.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="KLH46_001855"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="KLH46_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBE0378557.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="KLH46_001856"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="KLH46_001856"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBE0378558.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="KLH46_001857"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="KLH46_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBE0378559.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="KLH46_001858"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="KLH46_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBE0378560.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="KLH46_001859"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="KLH46_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBE0378561.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="KLH46_001860"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="KLH46_001860"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBE0378562.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="KLH46_001861"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="KLH46_001861"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBE0378563.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="KLH46_001862"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="KLH46_001862"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBE0378564.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="KLH46_001863"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="KLH46_001863"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBE0378565.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="KLH46_001864"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="KLH46_001864"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBE0378566.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="KLH46_001865"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="KLH46_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBE0378567.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="KLH46_001866"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="KLH46_001866"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBE0378568.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="KLH46_001867"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="KLH46_001867"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBE0378569.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="KLH46_001868"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="KLH46_001868"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBE0378570.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGNVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="KLH46_001869"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="KLH46_001869"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBE0378571.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="KLH46_001870"
     CDS             complement(31267..32913)
                     /locus_tag="KLH46_001870"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBE0378572.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="KLH46_001871"
     CDS             complement(32916..32966)
                     /locus_tag="KLH46_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378573.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="KLH46_001872"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="KLH46_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBE0378574.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..35022
                     /locus_tag="KLH46_001873"
     CDS             33502..35022
                     /locus_tag="KLH46_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBE0378575.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="KLH46_001874"
     CDS             complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="KLH46_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBE0378576.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35504..36343
                     /gene="hdfR"
                     /locus_tag="KLH46_001875"
     CDS             35504..36343
                     /gene="hdfR"
                     /locus_tag="KLH46_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBE0378577.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36439..36514)
                     /locus_tag="KLH46_001876"
     tRNA            complement(36439..36514)
                     /locus_tag="KLH46_001876"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36479..36481),aa:Trp,seq:cca)
BASE COUNT         8918 a         9996 c         9200 g         8406 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgccgg tactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcatat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgtacc
     6361 tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 gcgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acgggcgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg gaatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccgatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acattaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcaaccg gctctcgggg
    34621 ccctttctcg accgcttcga tctctcactg gagatcccat taccaccccc cggcattttg
    34681 agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt aatggccgcc
    34741 agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag tccggaaata
    34801 cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaagaaac gctgatccat
    34861 ctggggttat cgattcgtgc ctggcagcgg ttactgaaag ttgcacgaac cattgctgat
    34921 attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag ctatcgtgcg
    34981 attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg catttcgccc
    35041 tttttattaa tcgtcagaat cggtgtagtc ttcagcacct tcaacctgcg gtttaccgcc
    35101 ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg accacacgcg
    35161 ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga gtttctcttc
    35221 ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac catgacgctc
    35281 aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac gtggataatg
    35341 tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc acgctcctaa
    35401 ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga gtgtaaaaca
    35461 aaacatttaa atcataacga caaataattt tgcggagagc actgtggata cggaattgtt
    35521 aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg aatcgctcta
    35581 tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac tgggtgtgaa
    35641 ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa aactactgcc
    35701 ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg cgcatacctc
    35761 acgacataac gagttttcta tcggtgccag cgcctcgttg tgggaatgta tgcttaatca
    35821 gtggttggga cgcttgtatc aaaatcagga tgcccataca ggcttacagt tcgaagcgcg
    35881 aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg atcttcttat
    35941 taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctggggt atttcacttt
    36001 agcgctttat accagtgccc cttctaaact aaagggagat cttaactatc tgcgactgga
    36061 gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg aagttcccat
    36121 tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta atggttgcac
    36181 ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg tcgatagcac
    36241 aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa atgcgttgat
    36301 tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat taatctgaat ggctggcaag
    36361 gatgccggta gaaggattta cttcggagag gattatttca gataaaaaaa atccttagct
    36421 ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg
    36481 gagaccggtg ctctaccaat tgaactacgc ccctaattag
//
LOCUS       DAEEGA010000035        34993 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEEGA010000035 DAEEGA010000000
VERSION     DAEEGA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34993
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.035"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..809)
                     /locus_tag="KLH46_001877"
     CDS             complement(<1..809)
                     /locus_tag="KLH46_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBE0378578.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLT"
     gene            901..987
                     /locus_tag="KLH46_001878"
                     /pseudo
     CDS             901..987
                     /locus_tag="KLH46_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1392..1868)
                     /locus_tag="KLH46_001879"
     CDS             complement(1392..1868)
                     /locus_tag="KLH46_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBE0378579.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1927..3216)
                     /gene="bioA"
                     /locus_tag="KLH46_001880"
     CDS             complement(1927..3216)
                     /gene="bioA"
                     /locus_tag="KLH46_001880"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBE0378580.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3303..4343
                     /gene="bioB"
                     /locus_tag="KLH46_001881"
     CDS             3303..4343
                     /gene="bioB"
                     /locus_tag="KLH46_001881"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBE0378581.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4340..5494
                     /gene="bioF"
                     /locus_tag="KLH46_001882"
     CDS             4340..5494
                     /gene="bioF"
                     /locus_tag="KLH46_001882"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBE0378582.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5481..6236
                     /gene="bioC"
                     /locus_tag="KLH46_001883"
     CDS             5481..6236
                     /gene="bioC"
                     /locus_tag="KLH46_001883"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBE0378583.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6229..6906
                     /gene="bioD"
                     /locus_tag="KLH46_001884"
     CDS             6229..6906
                     /gene="bioD"
                     /locus_tag="KLH46_001884"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBE0378584.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7485..9506
                     /gene="uvrB"
                     /locus_tag="KLH46_001885"
     CDS             7485..9506
                     /gene="uvrB"
                     /locus_tag="KLH46_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBE0378585.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLAEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9698..10606)
                     /gene="yvcK"
                     /locus_tag="KLH46_001886"
     CDS             complement(9698..10606)
                     /gene="yvcK"
                     /locus_tag="KLH46_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBE0378586.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10871..11015
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11003..11992
                     /gene="moaA"
                     /locus_tag="KLH46_001887"
     CDS             11003..11992
                     /gene="moaA"
                     /locus_tag="KLH46_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBE0378587.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12014..12526
                     /gene="moaB"
                     /locus_tag="KLH46_001888"
     CDS             12014..12526
                     /gene="moaB"
                     /locus_tag="KLH46_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBE0378588.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12529..13014
                     /gene="moaC"
                     /locus_tag="KLH46_001889"
     CDS             12529..13014
                     /gene="moaC"
                     /locus_tag="KLH46_001889"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBE0378589.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13007..13252
                     /gene="moaD"
                     /locus_tag="KLH46_001890"
     CDS             13007..13252
                     /gene="moaD"
                     /locus_tag="KLH46_001890"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBE0378590.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13254..13706
                     /gene="moaE"
                     /locus_tag="KLH46_001891"
     CDS             13254..13706
                     /gene="moaE"
                     /locus_tag="KLH46_001891"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBE0378591.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13843..14547
                     /locus_tag="KLH46_001892"
     CDS             13843..14547
                     /locus_tag="KLH46_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBE0378592.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14752..15465
                     /locus_tag="KLH46_001893"
     CDS             14752..15465
                     /locus_tag="KLH46_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBE0378593.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLEQAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15501..16457)
                     /locus_tag="KLH46_001894"
     CDS             complement(15501..16457)
                     /locus_tag="KLH46_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBE0378594.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16457..17698)
                     /gene="clsB"
                     /locus_tag="KLH46_001895"
     CDS             complement(16457..17698)
                     /gene="clsB"
                     /locus_tag="KLH46_001895"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBE0378595.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGIQVFEYRRRPLHGKVALMVDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17695..18456)
                     /locus_tag="KLH46_001896"
     CDS             complement(17695..18456)
                     /locus_tag="KLH46_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBE0378596.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18589..18999
                     /locus_tag="KLH46_001897"
     CDS             18589..18999
                     /locus_tag="KLH46_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBE0378597.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18961..20067)
                     /locus_tag="KLH46_001898"
     CDS             complement(18961..20067)
                     /locus_tag="KLH46_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0378598.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20078..21211)
                     /locus_tag="KLH46_001899"
     CDS             complement(20078..21211)
                     /locus_tag="KLH46_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0378599.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21204..22940)
                     /locus_tag="KLH46_001900"
     CDS             complement(21204..22940)
                     /locus_tag="KLH46_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0378600.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22933..23928)
                     /gene="hlyD"
                     /locus_tag="KLH46_001901"
     CDS             complement(22933..23928)
                     /gene="hlyD"
                     /locus_tag="KLH46_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBE0378601.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYNLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23931..24602)
                     /gene="cecR"
                     /locus_tag="KLH46_001902"
     CDS             complement(23931..24602)
                     /gene="cecR"
                     /locus_tag="KLH46_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBE0378602.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24831..26018
                     /gene="rhlE"
                     /locus_tag="KLH46_001903"
                     /pseudo
     CDS             24831..26018
                     /gene="rhlE"
                     /locus_tag="KLH46_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26225..26707)
                     /gene="ybiA"
                     /locus_tag="KLH46_001904"
     CDS             complement(26225..26707)
                     /gene="ybiA"
                     /locus_tag="KLH46_001904"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HBE0378603.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26827..28977
                     /gene="dinG"
                     /locus_tag="KLH46_001905"
     CDS             26827..28977
                     /gene="dinG"
                     /locus_tag="KLH46_001905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBE0378604.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29005..29967
                     /gene="ybiB"
                     /locus_tag="KLH46_001906"
     CDS             29005..29967
                     /gene="ybiB"
                     /locus_tag="KLH46_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBE0378605.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30108..31193
                     /gene="hcxB"
                     /locus_tag="KLH46_001907"
     CDS             30108..31193
                     /gene="hcxB"
                     /locus_tag="KLH46_001907"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBE0378606.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31422..31682)
                     /locus_tag="KLH46_001908"
     CDS             complement(31422..31682)
                     /locus_tag="KLH46_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE0378607.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31947..32213)
                     /gene="ybiI"
                     /locus_tag="KLH46_001909"
     CDS             complement(31947..32213)
                     /gene="ybiI"
                     /locus_tag="KLH46_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBE0378608.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32287..32964)
                     /gene="ybiX"
                     /locus_tag="KLH46_001910"
     CDS             complement(32287..32964)
                     /gene="ybiX"
                     /locus_tag="KLH46_001910"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBE0378609.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33006..34993)
                     /locus_tag="KLH46_001911"
                     /pseudo
     CDS             complement(33006..34993)
                     /locus_tag="KLH46_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8293 a         8975 c         9454 g         8271 t
ORIGIN      
        1 gttaatcttt gcagggcttg cagagtgcga gtcgacaatg gattgttata aatattaaca
       61 tctgcataaa tgtgcagatt catgatactg tctggcaatg tgttcagctg attgttaggg
      121 atatatagtg attgcagacg ttgtggaagt tcgggcagtc tgtgtagttg gttttcccca
      181 acgtttaaaa gcctcagatt ttgaggaagg ggcggtagct cttgtaatct gttatcaaaa
      241 gcacttagtt cctgtagttc acatggtaat tctggtaagg cagtaagctg gttgttgcca
      301 acagaaagtt tttgtagagc cgggggaaga tcaggtagcg tctccaggct attgagaagg
      361 gctgagagtg actgcaagga agatggaaga gaaggcaaac atgttaaaac tctgttaagt
      421 gaaacatcaa gagcgaccag gtgaggcgga agagccggga gcctgctcag tctgttatcg
      481 ctggctttaa gtacagttaa ggacggaggg agttctggca gagagcgtag ctcattgcta
      541 gagatgttaa gttcttgtat gtgcgggggc aggtatggga gagagcgtaa tctgagtaaa
      601 cttaaattga gggctggctc ttgaaaagcc agacatattt tcagtaatcg aactgcctgt
      661 gttcggtctt ctgttgcagc accttccttg gcccagttat cccagatgcg gtgataatcc
      721 gtgatatttt gctcttctac ggataaacgg gatatacatc ctaaaccaca aaaggagcgg
      781 aaaaaagaag gattgtgggc aggtttcatt tattattgat cctcattgtt tatatattaa
      841 tatattcgtg caaatggagt gaggattctt taaaaaaacg cgcctgtaaa tagttaagaa
      901 caggaagaac tgttggggat gtggctggcc gaaaatgaag gggtgaagtt ctggctcaat
      961 gtgctgactg aactgaaaaa acgcggttgg tcgccctacc ttttcatcaa gccagtagtc
     1021 tgcccaccac agcatcattt ctctgcgctt atcgagatac tgagcatggt tgtaaattca
     1081 gctaacccag tgcgttgaca aaaaattagc gcaagaggac aaaaaatcac cttgcgctaa
     1141 tgctctgtat caggtcacta ataccatcta agtggttgat tcatagtgac tggatatgtt
     1201 gtgttttgca gtattatgca gtctattttt taagctaaat gtattataat gcattgatat
     1261 taatgatttt tactgtttct cggtcagctt ttttatacta acttgagcga aacgggaagg
     1321 taaaaagaca aaaagttgtt tttaatacct ttaagtgata ccagatggca ttgcgccatc
     1381 tggcagagtg attaactaaa catcgcggta atcgatgcgc ttgccagaga gtggaaatga
     1441 acgttaaacc cgaccatcgc gccgctggct ccttcatcga catcaatacg ctctacatcc
     1501 agcgcgtgaa cggtaaaaat gtagcgatgg gtttcgcctt tcggcggcgc tgcgccatcg
     1561 tatccggttt taccaaaatc ggtacgcgtc tgcaaaacgc cgtctggcat tgctaccaga
     1621 ccagagccaa acccttgcgg taatacgcgg gtatcagcgg gtaaattaac aactacccag
     1681 tgccaccagc cggagccggt tggcgcatcc gggtcgtagc aggtgacaac aaaacttttc
     1741 gttcccgcag gaacatcatc ccacgccaga tgcggtgaaa tattatcgcc atcgtaaccc
     1801 atgccgttaa agacatgacg atgcggcaac ttatcgccat cgcgcagatc gttactgatg
     1861 agtttcatga accctccttt cttgtttgca gaaagtgtag ccagaaaccc tcacgctgac
     1921 ttcccgttat tggcaaaaaa atgtttcatc ctgtaccgcg cggttaaccg ctgcggtcag
     1981 acgctgcaac tgttgcggga gaataatata gggcggcatc aggtaaatca gtttgccaaa
     2041 aggccggatc cataccccct gttcgacaaa gaatttttgc agcgccgcca tattcaccgg
     2101 acgagtggtt tcgaccacac cgattgcccc cagtacgcgc acatcggcaa ccatttcggc
     2161 atcacgggct ggtgccagtt gctcgcgcag ctgcacttca atagccgcca cctgctgctg
     2221 ccattcgccg gattcgataa tcgctaggct ggcgtttgct gccgcgcagg ccagcggatt
     2281 gcccataaaa gttggcccat gcataaagca gccggcttcg ccgttactga tggtttctgc
     2341 aacctcgcgc gtggtaagtg tggcggaaag ggtcattgtg ccgccggtta aggctttacc
     2401 gaggcacaaa atgtccggcg cgatttctgc atattcacag gcaaacagtt tgccggtacg
     2461 accaaatccg gtggcgatct cgtcggcaat cagcaagata ccttcgcgat cgcacatttt
     2521 gcggattcgt tttaaccatt ccggatggta catgcgcatc ccgcctgcgc cctggacaat
     2581 cggctcaatg atcaccgctg cgatttcatg acgatgcgcc gccatcaggc gggcaaaacc
     2641 aaccatatcg cgttcatccc attcaccatc catgcggctt tgcggggcgg gagcaaacaa
     2701 gttttctggc agatagcctt tccacaggct gtgcattgag ttgtccggat cgcacaccga
     2761 catcgcgcca aaggtatcgc catgataacc attgcggaag gtcagaaaac gctggcgcgc
     2821 ttcgcctttg gcttgccagt actgcaacgc cattttcatc gccacttcca ccgctacgga
     2881 accggagtcc gcgagaaaaa cgcactccag cggttgcggc gtcattgcca ccagtttgcg
     2941 gcatagctcg atggcgggcg catgggtgat accgccaaac atcacatgcg acatggcatc
     3001 aatttgcgtc ttcatcgccg cattaagctg cgggtgattg tagccgtgga tcgccgccca
     3061 ccaggacgac ataccgtcaa ccaggcgtct gccgtcagac aaaattagct cgcaaccttc
     3121 ggcgctcgcc accggataaa ccggcagagg ggaggtcatg gatgtgtatg ggtgccagat
     3181 atggcgttgg tcaaaggcaa gatcgtccgt tgtcataatc gacttgtaaa ccaaattgaa
     3241 aagatttagg tttacaagcc tacaccgaat taacaacaaa aaacacgttt tggagaagcc
     3301 ccatggctca ccgcccacgc tggacattgt cgcaagtcac agaattattt gaaaaaccgt
     3361 tgctggatct gctgtttgaa gcgcagcagg tgcatcgcca gcatttcgaa cctcgtcagg
     3421 tgcaggtcag cacgttgctg tcgattaaga ccggagcttg tccggaagat tgcaaatact
     3481 gcccgcaaag ctcgcgctac aaaaccgggc tggaagccga gcggttgatg gaagttgaac
     3541 aggtgctgga gtcggcgcgc aaagcgaaag cggcaggatc gacgcgcttc tgtatgggcg
     3601 cggcatggaa gaatccccac gaacgcgata tgccgtacct ggaacaaatg gtgcaggggg
     3661 taaaagcgat ggggctggag gcgtgtatga cgctgggcac gttgagtgaa tctcaggcgc
     3721 agcgcctcgc gaacgccggg ctggattact acaaccacaa cctggacacc tcgccggagt
     3781 tttacggcaa tatcatcacc acacgcactt atcaggaacg cctcgatacg ctggaaaaag
     3841 tgcgcgatgc cgggatcaaa gtctgctcag gtggcattgt gggcttaggc gaaacggtaa
     3901 aagatcgcgc cggattattg ctgcaactgg caaacctgcc gacgccgccg gaaagcgtac
     3961 caatcaacat gctggtgaag gtgaaaggca cgccgcttgc cgataacgat gacgttgatg
     4021 cctttgattt tattcgcacc attgccgtag cgcggatcat gatgccgacc tcttacgtgc
     4081 gcctttctgc cggacgcgag cagatgaacg aacagactca ggcgatgtgc tttatggcag
     4141 gcgcaaactc gattttctac ggttgcaaac tgctgaccac gccgaatccg gaagaagata
     4201 aagacctgca actgttccgc aaactggggc taaatccgca gcaaactgcc gtgctggcag
     4261 gggataacga acaacagcaa cgtcttggac aggcgctgat gaccccggac accgacgaat
     4321 attacaacgc ggcagcatta tgagctggca ggagaaaatc aacgcggcgc tcgatgcgcg
     4381 gcgtgctgcc gatgccctgc gtcgccgtta tccggtggcg caaggagccg gacgctggct
     4441 ggtggcggat gatcgccagt atctgaactt ttccagtaac gattatttag gtttaagcca
     4501 tcatccgcaa attatccgtg cctggaagca gggggcggag caatttggca tcggtagcgg
     4561 cggctccggt cacgtcagcg gttatagcgt ggtgcatcag gcgctggaag aagaactggc
     4621 cgagtggctt ggctattcgc gggcgctgct gtttatctct ggttttgccg cgaatcaggc
     4681 agtcattgcc gcgatgatgg cgaaagagga ccgtattgtt gccgaccggc ttagccatgc
     4741 ctcattgctg gaggctgcca gtttaagccc gtcgcagctt cgccgtttta ctcataacga
     4801 tgtcgctcat ctggcgcgac tgcttgcttc cccctgtccg gggcagcaac tggtggtgac
     4861 agaaggcgtg ttcagcatgg acggcgatag cgcgccactg gcggaaatcc agcaggtaac
     4921 gcaacagcac aatggctggt tgatggtcga tgatgcccac ggcacgggcg ttatcgggga
     4981 gcaggggcgc ggcagctgct ggctgcaaaa ggtaaaacca gaattgctgg tagtgacttt
     5041 tggcaaagga tttggcgtca gtggggctgc ggtgctttgc tcaagtacgg tggcggatta
     5101 tctgctgcaa ttcgcccgcc accttatcta cagcaccagt atgccgcccg ctcaggcgca
     5161 ggcattacgt gcgtcgctgg cggtcattcg ccgtgatgag ggggatgcac ggcgcgaaaa
     5221 actggtgtca ctcattgcgc gttttcgtgc cggagtacag gatttgccgt ttacgctggc
     5281 tgattcatgc agcgccatcc agccattgat cgtcggtgat aacagccgtg cgttacaact
     5341 ggcagaaaaa ctgcgccagc aaggctgctg ggtcacggcg attcgcccgc caaccgtacc
     5401 cgctggtact gcgcgactgc gcttaacgct aaccgctgcg catgaaatgc aggatatcga
     5461 ccgtctgctg gaggtgctgc atggcaacgg ttaataaaca agccattgca gcggcatttg
     5521 gtcgggcagc cgcacactat gagcaacatg cagatctaca gcgccagagt gctgacgcct
     5581 tactggcaat gcttccacag cgtaaataca cccacgtact ggacgcgggt tgtggacctg
     5641 gctggatgag ccgccactgg cgggaacgtc acgcgcaggt gacggcgtta gatctctcgc
     5701 cgccaatgct tgcgcaagcc cgccataaag atgccgcaga tcattatctg gcaggagata
     5761 tcgaatccct gccgttagcg actgcgacgt tcgatcttgc atggagcaat ctcgcggtgc
     5821 agtggtgcgg taatttatcc acggcactcc gcgagctgta tcgggtggtg cgccccggag
     5881 gcgtggtcgc gtttaccacg ctggtgcagg gatcgttacc cgaactgcat caggcgcggc
     5941 aggcggtgga cgagcgtccg catgctaatc gctttttacc gccagatgaa atcgaagagt
     6001 cgctgaacgg cgtgcgttat caacatcata ttcagctcat cacactatgg tttgatgatg
     6061 cgctcagtgc catgcgttcg ctgaaaggca tcggtgctac gcatcttcat gaagggcgcg
     6121 acccgcgaat attaacgcgt tcgcagttgc agcgattgca actggcctgg ccgcaacaac
     6181 aggggcgata tcctctgacg tatcatcttt ttttgggagt gattgctcgt gagtaaacgt
     6241 tattttgtca ccggaacgga taccgaagtg gggaaaaccg tcgccagttg tgcactttta
     6301 caagccgcga aggcagtagg ttatcggacg gcaggttata aaccggtcgc ctctggcagc
     6361 gaaaagaccc cggaaggctt acgtaatagt gacgcgctgg cgttacagca caacagcagc
     6421 ctgcagctgg attacgcaac ggtaaatcct tacaccttcg cagaacccac ctcgccgcac
     6481 atcatcagcg cccaagaggg caggccgata gaatcattgg taatgagcgc cggattacgc
     6541 gcgcttgaac aacaggctga ctgggtgtta gtggaaggtg ctggcggctg gtttacgccg
     6601 ctttctgaca ctttcacttt tgcagattgg gtaacacagg aacaactgcc ggtgatactg
     6661 gtagttggtg tgaaactcgg ctgtattaat cacgcgatgt tgactgcgca ggcaatacaa
     6721 cacgccggac tgactctggc gggttgggtg gcgaacgatg ttacgcctcc gggaaaacgt
     6781 cacgctgaat atatgaccac gctcacccgc atgattcccg cgccgctgct gggagagatc
     6841 ccctggcttg cagaaaatcc agaaaatgcg gcaaccggaa agtacataaa ccttgccttg
     6901 ttgtagccat tctgtatttg gttaaattgc gagcgagatc gcgtcttcga ttgactgcaa
     6961 tttaaccaat taaattctaa aataatcacg aaaaaaattt tacttccgcc tcatgcggcg
     7021 aatgtgggaa ttgcccaggc ggcgggggat aggggctgga gacagttatc cactattcct
     7081 gtggataacc atgtgtatta gagttagaaa acacgaggca agcgagagaa tacgcggctt
     7141 gcacgcgaat tggcgttaaa gacggctcaa agaaatatct tttatttttt aactggttag
     7201 ataaatgcaa tggcagtcac tgaacaggca tctcttgcca taaaactgtc atcactcatc
     7261 ttgacaaatg ttaaaaaagc cgttgctttg gggataaccc ggtaaggctg gagttttatc
     7321 tcgccacaga gtaaattttg ctcatgattg acagcggagt ttacgctgta tcagaaatat
     7381 tatggtgatg aactgttttt ttatccagta taatttgttg gcataattaa gtacgacgag
     7441 taaaattaca tacctgcccg cccaactcct tcaggtagcg actcatgagt aaaccgttca
     7501 aactgaattc cgcttttaaa ccttctggcg atcagccaga ggcgattcga cgtctcgaag
     7561 aggggctgga agatggccta gcgcaccaga cgttacttgg cgtgactggc tccgggaaaa
     7621 ccttcaccat tgccaatgtc attgctgacc ttcagcgccc aaccatggta cttgcgccca
     7681 acaaaacgct ggcggcccag ctgtatggcg aaatgaaaga gttcttcccg gaaaacgcgg
     7741 tggagtattt cgtctcctac tacgactact atcagccgga agcctatgta ccgagttccg
     7801 acaccttcat tgagaaagat gcctcggtta acgaacatat tgagcagatg cgtttgtccg
     7861 ccaccaaagc gatgctggag cggcgtgatg tgattgtagt ggcgtctgtt tccgcgattt
     7921 atggtctggg cgatcctgat ttatatctca agatgatgct ccatctcacg gtcggtatga
     7981 ttatcgatca gcgcgcgatt ctgcgccgac tggcggagct gcaatacgct cgtaatgatc
     8041 aagcattcca gcgtggtact ttccgcgttc gtggcgaggt gatagatatc ttcccggcag
     8101 aatcggatga cattgcactt cgcgtggaac tgtttgacga ggaagtggaa cgattgtcgt
     8161 tatttgaccc gctgaccggg cagattgttt ccactattcc acgttttacc atctacccga
     8221 aaacgcacta cgtcacaccg cgcgagcgca tcgtacaggc gatggaggag atcaaagaag
     8281 aactggccgc cagacgcaaa gtgctgttgg aaaacaacaa actgctggaa gagcagcggc
     8341 tgacccagcg tacccagttt gatctggaga tgatggacga gctgggctac tgttcgggga
     8401 ttgaaaacta ctcgcgcttc ctctccggtc gtggaccggg tgagccaccg ccgacgctgt
     8461 ttgattacct gcctgccgat gggctgctgg tcgtcgatga atctcacgtc accattccac
     8521 aaattggcgg catgtatcgc ggtgaccggg cgcgtaaaga gacactggtg gagtacggct
     8581 tccgcctgcc atcagcgctg gataaccgtc cgcttaagtt tgaagagttc gaagcattag
     8641 cgccgcaaac catctatgtt tcggcgacgc cgggtaatta cgagctggaa aaatccggcg
     8701 gcgatgtggt ggatcaggtg gtgcgtccaa ccggattgct tgacccgatt atcgaagtgc
     8761 ggccggtggc gacacaggtt gatgatcttc tttcggagat tcgtcagcga gcggcaatta
     8821 acgaacgcgt gctggtcacc acactgacca agcgaatggc ggaagatctt gccgaatatc
     8881 tcgaagaaca cggtgagcgc gtgcgttatc ttcactcaga tatcgacacc gtcgagcgta
     8941 tggagattat ccgcgacttg cgtctgggtg agttcgacgt attggtaggg atcaacttac
     9001 tgcgtgaagg tctggatatg ccggaagtgt cgctggtggc gatcctcgac gctgacaaag
     9061 aaggcttcct gcgttccgaa cgttcgttga tccagaccat tggtcgtgcg gcacgtaacg
     9121 ttaacggtaa agcgattctc tacggcgata ggagcactcc atcaatggcg aaagcgattg
     9181 gcgaaaccga acgtcgccgt gagaaacagc agaagtacaa cgaggaacac ggcattacgc
     9241 cgcaaggctt gaacaagaaa gtggtcgata tcctggcgct ggggcagaac attgccaaaa
     9301 ccaaagcgaa gggcagagga aaatcgcgcc cgattgttga gccggataat gtgccgatgg
     9361 atatgttgcc gaaagcgttg cagcagaaaa tccatgaact ggaagggctg atgatgcaac
     9421 acgcgcagaa tctggagttc gaagaagcgg cacaaattcg tgaccagttg catcagttgc
     9481 gtgagttgtt tattgccgca tcgtaacagg atagcgaaga agactgatga caaacggaaa
     9541 acagtctgat gcgctacgct tatcaggcct acattttctc cgcaatatat tgaatttgcg
     9601 cggtttgtag gccggataag gcgttcacgc cgcatccggc ataaacaacg cgcactttgt
     9661 cttcaatagg aagccggaat tttccttcgg atttccgtta acctaaagcc tgtaacgcct
     9721 tttccagcgc gttatgtaac aactggcggt catgacgata cggaatatcg ctggcctcca
     9781 gtacctcctg gatcacaatc cgctctttca ccgccgagac atccactttt ggcccgacga
     9841 tgaccgcatc aatgaccttt ttaccaacat actgctccat aattgccagc ttgctttcca
     9901 gcttcaaatt agccgcaggt aaactcaact cacgccccag attgccgata taaaccatcg
     9961 gcgctggcgt gcggcgtaat gcctgggcga tttccttcag cagcagaatt ggcatcaggc
    10021 tggtataaaa actgccaggc ccaataatga tgagatccgc ttcattgata gcgtgaaccg
    10081 cctcacgcgt tgcgggtaca ttaggcgtta acaataactc ttgaatcggc gtagttaact
    10141 ggtcgatatt gacctcgccg taaacttcat gcccctgatc gtcaatcgcc atcagatcaa
    10201 caggatgctc tgacattgga atcaaatgcg tatccacttt cagcagatta cgaattaaat
    10261 tgatggcttc cagaggccgc acgctaaggt gatccagcgc ctttaacatc aagtttccga
    10321 gattatgacc ggaaagttcg ccattgccgc caaaacggta ttcaaacatc gcggaggcga
    10381 cgttcggttc cgttatcagc tggttgaggc agttgcgcat atcgccccaa gcaatgccgc
    10441 cttctgaacg gcgaatacgc cccgtcgagc caccattatc cgtggtggtg acgatacccg
    10501 ttaaacgaga acccaaagac gaaagtgatg agagaacgcg tcccagtcca tgccctccgc
    10561 cgagagcaac gacacgatca agatcagcca gcgtacgatt gcgcatatag tttcctggag
    10621 tcagattatc cgcgctacag tagcgcaaat tgcggggaaa cagcaattaa cctgccaata
    10681 ttgaggatac aaactatttt cttctatctc taaaggacga tgcacgctat gcctccctga
    10741 tgatgtatat caaagtaaaa ccgccatttt cccttattct gtagcgaaat agcacgatca
    10801 tgacgctata tacatgatta catagcgaaa gtgtggatgg taaaaatctc atttacacgc
    10861 tagtatcggc ttaaccacta aacactctag cctctgcacc tgggtcaact gatacggtgc
    10921 tttggccgtg acaatgctcg taaagattgc caccagggcg aaggaagaaa tgacttcgcc
    10981 tcccgtatct ggaaaggtgt acatggcttc acaactgact gatgcatttg cgcgtaagtt
    11041 ttactacttg cgcctgtcga ttaccgatgt gtgtaacttt cgttgcacct actgcctgcc
    11101 ggatggctac aaaccgagcg gcgtcaccaa taaaggcttt cttaccgtcg atgaaattcg
    11161 ccgggttacg cgcgccttcg ccagtctggg caccgaaaaa gtgcgcctga caggaggaga
    11221 gccgtcttta cgccgcgact ttaccgatat catcgccgct gtgcgggaaa acgacgctat
    11281 ccgccagatt gcggtcacca ccaatggtta ccgtctggaa cgcgatgtgg cgaactggcg
    11341 cgatgcggga cttactggca ttaacgtcag tgtcgacagt ttggacgccc gccagtttca
    11401 cgctattacc gggcaggata aattcaacca ggtcatggca gggattgatg ctgcatttga
    11461 ggccggtttt gagaaggtca aagtcaatac cgtgctgatg cgtgatgtta atcatcacca
    11521 gctcgacacc tttctgaact ggatccagca tcgccctatc cagctgcgtt tcatcgaact
    11581 gatggaaacg ggcgagggca gcgagctctt ccgtaagcat cacatctctg gtcaggttct
    11641 gcgtgacgag ctactgcgtc gcggctggat ccaccaatta cgtcaacgca gcgacggtcc
    11701 cgcgcaagtc ttttgccatc cagattacgc cggagagatt ggccttatca tgccgtatga
    11761 aaaagacttc tgcgccactt gcaaccgcct gcgcgtttcc tccattggta aactccatct
    11821 ctgcctgttt ggtgaaggcg gcgttaacct gcgcgatctg ctggaaggcg atacccagca
    11881 acaggcgctg gaagcgcgta tttcagcggc gctgcgggag aagaaacaga cccatttcct
    11941 gcatcaaaac aacaccggta ttacgcaaaa cttatcgtac attggcggct aaaacgtcaa
    12001 aaggagagat cagatgagtc aggtaagcac tgaatttatc ccgacccgta ttgctattct
    12061 tacggtttct aatcgtcgcg gtgaagaaga cgatacctcc ggtcactatc tgcgcgattc
    12121 ggcgcaagaa gcgggccatc acgttgtcga tgaagccatt gtgaaagaaa accgctacgc
    12181 tattcgcgct caggtatctg cgtggatcgc cagcgacgat gtacaagtgg tattgattac
    12241 gggtggtact ggcctgacgg aaggtgatca ggctcccgaa gcgctgctgc cgttgttcga
    12301 ccgtgaggtt gaaggttttg gtgaagtgtt ccgtatgttg tcgtttgaag agattggcac
    12361 ttccacgttg caatctcgtg cggtagcggg cgtcgctaac aaaacgctga ttttcgccat
    12421 gccgggttcg accaaagcgt gccgtaccgc atgggaaaat atcatcgcgc cgcagctgga
    12481 tgcccgtacg cgtccgtgta atttccatcc acatttgaag aaataagtat gtcgcaactg
    12541 acccatatca acgccgctgg cgaagcgcac atggtggatg tctccgccaa agcggaaacc
    12601 gtgcgtgaag cgcgggcgga agcctttgtc accatgcgca gcgagacgct ggcgatgatt
    12661 attgatggtc gccaccacaa aggcgacgta tttgccactg cgcgtattgc cggtattcag
    12721 gcggcaaaac gcacttggga tctgatcccg ctgtgtcacc cgctgatgct cagcaaagtt
    12781 gaagtcaatt tacaggccga gccggagcac aatcgggtgc gtatagaaac cttatgccgc
    12841 ctgaccggga aaaccggtgt cgaaatggaa gcgttaaccg cggcctccgt ggcggcgctg
    12901 accatttatg acatgtgcaa agcggtgcaa aaagatatgg tgattggtcc ggtacgtttg
    12961 ctggcgaaga gcggcggcaa gtcgggtgac tttaaggtgg aagcggatga ttaaagttct
    13021 ttttttcgcc caggtgcgcg agttggtggg aacagatgca accgaagtgg ctgcggatta
    13081 cccaaccgtt gaagcgttac gccagcacct ggctgcgcag agcgatcgct gggcgctggc
    13141 gctggaagat ggcaaattgc tggctgccgt caaccagacg ctggtgagtt ttgaccatcc
    13201 gctgactgac ggcgacgaag tagctttctt cccgccggta accggaggtt aagatggcag
    13261 aaaccaaaat tgttgtcggc ccgcagccgt tcagcgtagg agaagagtac ccgtggctgg
    13321 cggagcgtga cgaagacggt gcggtagtca ccttcaccgg aaaggtgcgc aaccataacc
    13381 tgggcgacag cgtcaaagca ttaaccctcg aacactatcc ggggatgact gaaaaagcac
    13441 tggcagaaat tgttgatgaa gcgcgtaacc gctggccgct ggggcgcgtc acggtgattc
    13501 accgcatcgg ggaattatgg ccgggcgatg aaatcgtttt tgtcggtgtc accagtgcgc
    13561 atcgcagcag tgcgtttgaa gccgggcagt ttattatgga ttatctcaaa acccgcgcac
    13621 cgttctggaa gcgcgaagcc acgccggaag gcgaccgctg ggttgaagcc cgggagagcg
    13681 atctgcaggc ggcaaaacgc tggtagtttt ttgttagccg gataaggcac cgctgcgtcc
    13741 ggcaatttca ccgctcagta catgcgttta cgctgcgtcg atgcacagcc tcatcatttt
    13801 gcagtatcct taagatattc cttatatctt caggagatcg tcatggacag attcccacgt
    13861 tctgattcaa tcgtacaacc ccgggctggc ttgcaaactt atatggcgca agtctatggc
    13921 tggatgaccg ttggcttgtt gctgaccgca tttgttgcct ggtatgcggc taattccgcg
    13981 gccgtgatgg agctgttgtt cactaaccgt gtctttttaa tcggtctgat catcgcgcaa
    14041 ttagcattgg ttattgtgtt atcagcgatg attcaaaagc tgagcgcagg tgtaacgacg
    14101 atgctcttta tgctttattc ggcgctgacg ggtcttacgc tttccagtat attcattgtc
    14161 tataccgctg cttctatcgc cagtactttc gtcgttactg ccgggatgtt cggcgcaatg
    14221 agcctgtacg gttacaccac gaagcgcgat ttaagtggct ttggcaatat gctgtttatg
    14281 gcgttaatcg gcattgtgct ggcatcgctg gtcaacttct ggctgaaaag cgaagcattg
    14341 atgtgggcag ttacctacat cggcgtgatt gtctttgtcg gattgacggc gtatgacact
    14401 cagaaattga aaaatatggg tgagcagatt gatacccgcg acacgtcgaa cctgcgcaaa
    14461 tattccattc ttggtgcgtt aaccttgtat ctggacttca tcaacctgtt cctgatgttg
    14521 ttacggatct tcggcaaccg ccgttaattc tttttcacca tcactatccc ggccatgtgt
    14581 cgggattttt ttcttactaa aaacgacact taacaagtac caggtataaa atatgcctta
    14641 tgagcgagcg gaatcgtttt ccgtatccat aaatacaaaa gtattttgta tgcgtggttt
    14701 taaatttatt gatggtgaat attaatgttt tttctaagga caactaaaag aatggaatca
    14761 tactcgcaaa acagcaataa attagatttt cagcacgagg ccaggatatt aaacggtata
    14821 tggctcatta ccgctttagg tttggtggca accgcaggac tagcctgggg agctaagtat
    14881 ctcgaaatta cggcaaccaa atatgattca ccaccaatgt atgtcgccat agggttatta
    14941 ttactttgta tgtatggctt aagtaaggat atcaacaaga taaatgccgc catcgcgggc
    15001 gtaatatatc tgtttttact ctctttggtg gcgattgtcg ttgcaagttt agttcctgta
    15061 tatgccatta tcatcgtgtt cagcactgcg ggcgcgatgt ttttaatcag tatgctggcc
    15121 ggtttattat ttaatgttga tcctggttct caccgtttta tcattatgat gacgttgaca
    15181 gggttggccc tggtaatcat cgtgaatgcg gcattaatga gtgaacggcc catttgggta
    15241 ataagttgct taatgattgt gttatggcca ggcattatct cgcatggacg aaataagctc
    15301 cttgaacagg caggaaaatg ccatagtgaa gagttgtgga gtccggttcg ttgcgctttt
    15361 acaggtgcat taacactcta ttactatttt atcggcttct ttgggatact tgccgcgata
    15421 gctataacgc ttgtctggca aaggcatacg cgtttttttc attagtcgta aatgcagata
    15481 ctaaagaact taactaaact tcacatcgcc gcttcatttt tcgcccgtag cttcttcgct
    15541 tggctttcca gtagcaggta acagatcagc gccagcagca gcgggataaa gtaatacagg
    15601 acgcgataag cgagcagggc ggcgataatg ctccctttcg aggtatgctc cccagccagt
    15661 agtgcgataa acaccgcttc cagcacaccg atccccgccg gaatatgcac gatgacgcca
    15721 gcaatactac taaccagtaa cacgcccagt acaaagaaat agttcacgct ttgaccaagt
    15781 aacagccaga taatcgcccc cattaccatc cagttaacgc tggaaatcag catctgagcg
    15841 agggcgaatt tccatgaagg cagcaccagc ttttgccctt tgatggtcat atggcggtgc
    15901 ttcgcgaaag cgcaaaacca caaataaacc gcgataatca tcagtaagcc aatgccgaga
    15961 atacgcagcg tagtttgatc gacataccag taatccggca actccaccac gcctgcggta
    16021 aagataatcc ctgccagtaa aatatagccc agccagttgg tggtaatact gagcgaggaa
    16081 atccgcgtaa tagtgctgcc cggtaaccct agtcgggagt acaaacgata gcgcatacca
    16141 atgccgccga cccaggtact gagcgtcagg ttgaaggcgt agcagataaa cgacaccagc
    16201 atcacctggc gcttcgccag tttgtgaccg cagtaaaagc gggcgagcag gtcatagcag
    16261 ccgtaaatca gatagctgac gaccaccagc ccgaccgcac taagcagcgc aacgcgattg
    16321 tagtcgcgga tgaccttcca gacctcttcc cagtccactt ttttggcgta gaccaccagc
    16381 aacacaatca ccgcgataaa aaacagccag gtgaggatct tctttgctaa gcgccagcgc
    16441 gggtgtgatt tactcatcag gggtttaccc ccgtgttttc agtttctacc cgatcctgcg
    16501 tttccattgt cggttgtgcg gggggatcaa cctgcgtcag acgtggtgtg tgtgccggaa
    16561 gccagccaac cagcgccggg aagtggcgta aaaagtggaa cgccagcacg cttttggtca
    16621 ggttccacca ggtgcgtttg ggcagcatgg tttcatccac ctgctgacaa tctgcggcaa
    16681 taatgccgtt cagattatcg cgcagcgtct ggttaaaatg acgatcgtgg atgatgacat
    16741 ttgcttcgag attcagtgac aaactgagcg gatcgagatt actggaccct actgtcgccc
    16801 agtgatcgac catcaatgcc actttgccgt ggagcgggcg gcggcggtac tcaaaaacct
    16861 gaatgccgcc tttaaccaga tagttataca gcaagcgcgc accgactctg acaatcggca
    16921 tatccggttc gccctgaatg atcagtttga tccgcacccc gcgccgtgcc gctttacgca
    16981 aggcgtgtaa aaatcgatag ccggggaaga agtaggcgtt ggcgataatc acttcccgct
    17041 gcgcctgagt gagcattttc agataatggc gttcaatatc atcgcgatgt tcttcgttat
    17101 cgcgccagac cagcaatacc tgcgcttctc ccggctggcg gttctcttcc gctttgtgat
    17161 gacgtcgcca ccagcgtcgt gccgcgctct gtccaggcag gttttccagc tcaaactgga
    17221 gaatatcttc gacaatcggc ccttcaaggc gtaccgcgta atcctgttta gcctctggac
    17281 cgtagctgga catatgctcg gcggagtaat tcagcccgcc aataaaggcg atacgcgcgt
    17341 cgatcaccac aattttgcga tgcatccggc gaaacacatt ggtgcgcata ccaaaaaggc
    17401 gagggcgggg atcgtagtag cggaacacta cgccagctgc cgtcagttca ttgacaaact
    17461 catcgctgag atccggcgaa ccgtagccat ccagcaagac ttccgcttta accccgcgtt
    17521 gcgctgctgc cagtagtgcc gcatgcagct gtttgccgac gttatcctca aaccagataa
    17581 acgtttcaag aatgatgcgt tcttgtgcct cgccaatcgc cttaaacacc gcgggataat
    17641 attgctcgcc gttttccagc aactggatct tattgccttc gcgccagcta catttcataa
    17701 atgaatctcc gcacttaaag gggcatgatc agaaaggtgt cgccatgtcc gcagcggcaa
    17761 cgcggttggc gcgctggcgc tggcattttt gacgtagatc ctgtccagtc gtagtagagg
    17821 aaattgcacc ggaaacgtgc gcgccgggcg tccgtgggcg cgggtaaaaa tctcatccag
    17881 tccggcctgc acttttaacg gatgattagc tttttgccgc cagtcattga aatcacccgc
    17941 caccaatacc ggttcgccgt ccggtagctc attcacccat tcggcgagca tcgcaagctg
    18001 cgcctgacgg tgcgcctcac gcaggcccag atgtacgcac atcacatgaa tcgcttttcc
    18061 ggtcatcggc ggcacaatgc ggcagtagag cacgccgcgc ttttccgcac catcgaccga
    18121 aacatcgcga ttctcataat gttcaatggg ataacgcgac agtacggcgt tgccgtgatg
    18181 cccttccggg tatacggcat tgcgaccgta ggcaaaatcg ctccacatag tgtcggcgag
    18241 aaactcgtag tgcgaggtat cgggccagtt ttccacatgc agcggatgaa cttcgtgcgc
    18301 gcccatcact tcctgcaggc aaacaatatc ggcgctgacg gtacgcacgg catcgcgaag
    18361 ttccggcaaa atgaagcgtc ggttaaacgc ggtaaagcct ttgtgaatgt tgatggtgag
    18421 caccttgaac gaaaattgtt gtgtttgatc gggcataatt ttcctgtctt tgcctcttat
    18481 ctcattgaaa tagtgtagtc ggcgtcacaa aaaggtgcgg tcttacggaa ttttccgtaa
    18541 agttcggtac tctgagtaag tagagataaa ttcttcagga gagaagccat gaagtggcaa
    18601 caacgtgttc gtgtcgcaac gggtctaagt tgctggcaga ttatgttgca tttactggta
    18661 gtggcgctgc tggtggtggg ctggatgagt aagactctgg ttcacgtcgg cgtgggatta
    18721 tgcgcactgt attgtgtcac ggtagtgatg atgctggtgt ttcagcgcca ccccgagcaa
    18781 cgctggcgtg aggtggcaga cgtgctggaa gagctgacca cgacctggta ttttggcgca
    18841 gcgctgattg tgctgtggct gttgtcccgc gtactggaaa acaacttttt gctggcaatt
    18901 gcagggctgg cgatccttgc tggcccggcg gtagtgtctt tgctggcgaa agataagaag
    18961 ttacatcacc ttacgtctaa acatcgcgta cgccgctgac cctgtcgtgg ccgttatcac
    19021 cagtagcggc cacaaactat tccacacaat atccagactc gcatccttca aataaatctg
    19081 cttggtaatg tccgtaaagt ggcgaatagg gttaatccac gtcagatttt gcagccacac
    19141 tggcatgttt tccaccggcg aaacgtaacc ggaaaggaga atggcgggca tcataaaaac
    19201 aaacacgccg ataaacgcct gctgttgtgt cgagcagagt gatgaaatca acagaccgaa
    19261 tcccaccagc gataaaccgt aaatcaccat cgtaaagtag aacagcgcta gcgatccggc
    19321 gaaggggatt tgatacgccc agataccaat cgccagcaca atggtggcct ggaaggtggc
    19381 gacaattaac gccggtacgg ctttgccgat gaagatctgc caggtggtga gcggcgaaac
    19441 cagtagctga tcgagcgtac cttgttcacg ttcgcgggcg acggaaagtg aagtgacgat
    19501 cattacgccg atagtggtga tcatggcgat cagcgacggc accacaaacc atttgtagtc
    19561 gagattcggg ttataccagt tgcgtaccac cagctcgctg ttgttgggtt tcggttttcc
    19621 ttccagcagc tcctgctgat aatttttgac gatctgttgc aggtagttgg cggcaatttg
    19681 cgcactgttg gagttacgcc cgtcgaggat caactgcaac ggcgcggtct ggaaggtatc
    19741 cagtttgcgc gagaagtcag ccgggaaacg caccagtagt aacgcctttt gtgtgtcgat
    19801 ggtcgggcgg atctcctgtg ggcttttcag cagcagcaca tgtgtaaagg cgctggcgcg
    19861 ggcaaaacgt tgagtaagtt ccaccgaatg ctcgccgtta tcttcatcgt agatggcgat
    19921 ggtggcgtta gtcacttcca gcgtggcggc gaacgggaac aggatcacct gaattagcac
    19981 gggtaaaatc agaatcgcgc gggtttgtgg ttcgcgcagc aacgactgca actctttgcg
    20041 gattaacgtc cataagcgat gaaacatgct cttctcccta atccagccga cgtttggttt
    20101 tcagccacgt caggccgata aacatcaccg ccgaagcgat caaaaacagc acgtttacca
    20161 ccagcaccac tggaatattc ccggcgagga acaggctttg cagggtgctg acgaaataac
    20221 gagcgggaat aatgtacgtc accgcgcgga tcaccgcggg catactgtcg atctgaaaaa
    20281 taaagccgga aagcataatc gacggcagaa aagcggcgtt cagggcgacc tgagcggcat
    20341 tgaactggtt gcgggtaatc gtggaaatca gcagccccat tcccagggta ctgagtaaaa
    20401 acaggctgga gataaaaaac agaatcagca gcgaaccgcg atacggcacg ccgagaataa
    20461 acactgacac cagcatacac agcaacatcg ccagcatccc gagaaagtaa taagggatca
    20521 gcttacacag cagcagttcc gtgcgggtaa tctccgtaga gagcagagcc tccatggtgc
    20581 cgcgttccca ttctcgcgcc accaccagcg aggtgagaat cgcgccgatg accgtcatga
    20641 taatggtcac cgcacccggg ataataaagt gctggcttat cgccgccggg ttaaaccagt
    20701 agcgggtttg tacatcaata agcggttcaa aagtctgccc gttgtcctcc gctcgctgca
    20761 tttgccagat ctgccagatc ccttcgacat acccctgcac aaagttagcg gtattcggct
    20821 cactgccgtc ggtgatcacc tgaatcggtg cggtggcgtt ggcgcgctcc atctgttcgg
    20881 cgaaatccac gggaataacc accagaccgc gaattttccc cgactgcatt ttggcgatca
    20941 gttcctgacg gttatcgctg atggtggcgt cgatgtaggg cgaaccggtc atggtgtggg
    21001 tgaaatccag cgccgcttcg ctacgctgtt ccagcaaaat cccgacccgc agcttgctgg
    21061 agtcgaggtt aatgccgtaa ccaaaaataa acagcagtag cagtgggatc actaccgcaa
    21121 tcagccagct actcggatcg cgaacgatct gccgcgtctc tttgacgcac agcgcccgca
    21181 cgcgatgcca ggaaaggatc gggttactca ttgctatgct ccttatccca gtcgtggatc
    21241 aactgaataa aggcttgctc catggtggga tcgggttgct catcgttagc tgactgtgct
    21301 ttcaaatcgt ccggcgtacc gctggcgatt aatttcccac ggtacaccag gccgatgcgg
    21361 tcgcaatatt ccgcttcatc cataaagtgg gtggtgacca tcaccgtgac gcctttctct
    21421 accatgctgt tgatgtgcag ccaaaattca cggcgggtga gggggtcaac gccggaagtc
    21481 ggttcgtcga gaaagagaat atccggttca tgcatcagcg aacaggccag cgccagccgc
    21541 tgtttaaaac ctaatggcag ttcatcggtg gcgtgtgagg caatactttt caggccaaat
    21601 gcctcgctca tacgggagat tttttcgttc tgcgcccgac cgcgtaagcc atacacacca
    21661 gagaaaaagc gtaaattctg ttcgaccgtc aggttgccgt agagcgaaaa tttttgcgcc
    21721 atatagccga gatgctggcg cgctttaccg gaactctctt tcagatccat ccccagcacc
    21781 agcgcctggc cggaagtcgg caccagcaaa ccgcacatca tcttaaaggt ggtcgattta
    21841 cccgcgccgt ttggccccag caaaccaaaa atctccccac gtttaacggc aaagttgacg
    21901 tgatcggtgg cggcaaaatc accaaatttc ttggtcagtt ctttcgcttc gatcaccgtc
    21961 tcgccgggtg tgccttctac cgtatgcaat attgcaccca gcggcgattc tgaggttccg
    22021 gcaccgccca gcaaatcaat aaacgcatct tcaaaacgcg gcgtagtttc gttgatgttg
    22081 atttccggca tcccgtcggc atggcgaata tcgtctggtg tggcctcttt tttgaggatc
    22141 agacgtaccg atttcccctg aatcatgcca tcgctgacct gcggcagttt caaggcgcgt
    22201 tgcaacagtt tgcggttgcc ctcgtgtgga ctggtcatca gaaagctgcg tccggccatg
    22261 gtttgtgtca gggcttttgg ttctccctga tacagcaact cgccttcgtt catcagtaac
    22321 acgtcacggc actgctcggc ttcgtcgaga tacgaggtac tccagaggat taacatccct
    22381 tcgcccgcca gctcatgcac catctgccac agttcgcgcc gtgagatagg gtcaacgccg
    22441 acgccgggtt catcgagcag caacactttc ggttcgccca ccagggtaca ggccaggccg
    22501 agtttttgtt tcatcccacc ggagagcttg cccgccaggc gtccggtaaa cggcccaaga
    22561 gacgtaaact ccagcaggcg agcaaaagtt tgcttacgtg cctcgccggt gacgctgcgc
    22621 aaatccgcgt acagattgag gttctccatc accgtgagat cttcatacag accaaatttc
    22681 tgcggcatat agccgagcac ggcgtgcagc gcgccgtcgt ttttgatcgg atcaaagcca
    22741 atcaccgtgg cactgccgct gtcgggtttc agtaatcccg ccaacatccg catcagtgtg
    22801 gttttacctg caccgtccgg ccccaccaac cccgtcacat aaccggcgtg aatggtacaa
    22861 tcgagcggcg cgacggcggg cttgtccatg cccggaaagc gtttttccag gccgttcagc
    22921 gtgataacgg catcattcat gtcctgcctc gtcaccgaat tgtaccgtca ctggcattcc
    22981 ctggcgtaac gcatcatcgg cgtcggtcac cacaatacgc aggcgataga cgaggtcggt
    23041 acgcagatcc ggtgtttcga cggttttcgg ggtaaattca gcagtcggcg aaacgaaacc
    23101 aatctgcccg tgatacggct tgtccgggcg accatcggta taaagcagca ctttgcgccc
    23161 cggctgggcc tggtcaagat tccgttcatc aacataagcg cgcacccaca ccggacgcgt
    23221 tagtgaaacg gtaaacaccg tgccaccttc attgagaacc gtgcctggct ctaccgcgcg
    23281 cgttaacagc gtgccatcag acggtgctat caacgttgag tcctgtaaat tcagctccgc
    23341 ctgcgccagt tgcgcctgcg cctgttcgag gctggctttc gcctgagcga tgtcctgttc
    23401 acggttaccg gaacggtact gacgcaattt atcctgtgct gatttcagcg ttgcctgtgc
    23461 ctggtcgcgc gaggagcggg cattttccag gtcatttgcc gaaatggtgc ggcttttcca
    23521 cagtccttgc tggcggttat agaagttctg cgcatagtca taggcggctt gcgcctgttt
    23581 caccgctgcg gcggcctgag cgatttcttc atcgcgatac ccggcaagca tcagattata
    23641 ctgcgcctgt gccaccgaga cgcccgcttt cgcctgcatc agggcaatct catacggctt
    23701 gtgatccagt tcgcccagca cctggcccgc tttgatagca tcaccttcgt ccaccgccag
    23761 cgattcaacg cgccccccaa cacggaaact aagattcacc gtacgaatat ccacgttgcc
    23821 atacagcgtc aggccgttat cctggcggct ttgataccac cagtagcctc cggcaaccac
    23881 ggcggcaagt accactaccg ccagtccgat cacgacaggt tttttcatca ctacaaactc
    23941 ctttgcgata atccttgcaa aatcaggtcg atatgacagg tcaccgtctg gttgatcagt
    24001 tcggtctttt cttcatcgaa cgcggtccag ccggtacgta acagaattgt ttctttgcca
    24061 agacggaacg ccagaatctc accaatcaac gcatgggtat gaaggatcat gcgggtgtca
    24121 ttagcgtcgc agccggtcca ggcggcaatc aggcgtgtca ggtggctgtg tagcggacta
    24181 atcacctgtt cgtgcaccag gtggtaggct gccgtgggag agagctgctc acgggagata
    24241 aacttgctga ggttgacggt gtcatcctgg gtgagcagct taatcatgtt cctgcaggcg
    24301 cgaaggatca gttcacggat ggcagcccga tcaggctgtg gttgggcgaa caagcgttcg
    24361 gcttcctcgg catgcggacg gaactgctcg ccaataaaat cggcaatcca ctgggcgcag
    24421 gcgaggtaca aatcttcttt cgaaccgaag tagtaggtga tggcagcgat attctgcccg
    24481 gcctgggcgg ctatctcgcg ggtggtggcg ttcattccat attcaccaaa ctgcgccagt
    24541 gcggcagcaa tcagctgttt tttcgcctgt tcacccttga ttgtcatggc aggattattc
    24601 atcgcacagt ccattcttaa tcaaatgatt gattaagatt atgactccat atgggagttg
    24661 tccagtatgg ctaagaattt tagctacgcc agtcacaggg ataatttatg cgctgcgtca
    24721 caaaaactgc tacactccgc tccctcatga cattgtggtt tttgtcattt tccttttcag
    24781 tatctccctg aaaactacac cggtaacggt cggggcggtt cggagtagtt atgtctttcg
    24841 attctttggg tttaagccct gatatcctgc gcgccgttgc cgagcagggt taccgtgaac
    24901 ccacccctat tcagcagcag gcgatccctg cggtgctgga aggccgcgac ctgatggcta
    24961 gcgcccagac cggcaccggc aaaacagcgg gctttacgct gccgctgttg caacacctga
    25021 tcactcgcca gccgcacgcc aaagggcgtc gtccggtacg tgcgctcatt cttaccccga
    25081 cccgtgaact ggcggcacag attggcgaaa acgtccgtga ttacagcaaa tacctgaaca
    25141 ttcgttcgct ggtggtgttt ggcggtgtca gcattaaccc acaaatgatg aagttgcgcg
    25201 gcggcgttga tgtgctggtg gcaacgccgg gacgtttgct ggacctggaa catcaaaatg
    25261 cagtgaagct ggatcaggtt gaaatcctcg tcctcgatga agctgaccgc atgctcgaca
    25321 tgggctttat ccacgatatc cgtcgcgtgt taacaaaact gccagcgaaa cgccagaacc
    25381 tgctgttctc tgcgaccttc tctgacgata ttaaagccct ggcggaaaaa ctgttgcaca
    25441 acccgctgga aatcgaagtg gcacgccgca ataccgcgtc tgatcaggtg acccagcacg
    25501 ttcactttgt cgataagaaa cgcaaacgcg aattgctgtc gcacatgatt ggtaaaggga
    25561 actggcagca ggtgctggtg tttacccgta ccaaacacgg cgctaaccat ctggctgagc
    25621 agctcaataa agatggcatc cgtagtgtgg cgatccacgg gaataaatcg caaggtgcgc
    25681 gtacccgtgc gctggctgat tttaaatcgg gtgacattcg cgtactggtg gcgaccgata
    25741 tcgctgcgcg cggtctggat atcgaagagt tgccgcacgt cgtcaaccat gaactgccaa
    25801 acgtaccgga agattatgtc caccgtatcg ggcgtaccgg tcgtgcggct gctaccggtg
    25861 aagcgttgtc gctggtgtgt gttgatgaac acaaactgct gcgtgatatc gaaaaactgc
    25921 tgaaaaaaga gatcccgcgc attgcgattc cgggctatga gccggacccg tcaatcaaag
    25981 ccgaaccgat ccagaacggt cgccagcaac gtggcggcat caggctgatg aacaaacgca
    26041 aaactgcctg atgcgctacg cttatcaggc ctacgtgaac tctgcaatat attgaatttg
    26101 catgcttttg taggccggat aaggcgttta cgccgcatcc ggcatttcac aacaagcact
    26161 tgtcagcaat ttgagaacac gggaaaaaat ttatctgttt taccgcccat aagccaccga
    26221 gacgttactt ctctatagcc aattgttcgc gcaactccat taaaaggtag cccagtcgat
    26281 tcttgccctt accattacca ccgtctcccc agtaagcatc gttttccgta tgctcaacca
    26341 gttttgcggg cgcggttgcc agcaagagca cacgcagttc tgcatgctgt tcgaatttag
    26401 cgcgaagtgc tttacgcatc acctgttctt taaccgactc ccagttttta cgaagtggct
    26461 tggaacgatt acgccccatg cgagcggcga ccatggggga agaaacccga cggatctctt
    26521 cgcggtattt ttcatcaagg aatttttgtg cctgaaaata gtgttcggag gtgggccagg
    26581 tattcccgtc aaccttgatg ggcctggcgg caaagttaga aaaatcaccg tagtcatcgc
    26641 tggtgctgta gaaatttatg atggtgtctt gcatgacgtg ttggatcctt tgtgctcgaa
    26701 cgggcattaa accgcattat gttggtggtt attgcgagcc gctttccaga aacagaaaaa
    26761 ccattacccc tgaaaaccga aaaatgccac aatattggct gtttatacag tatttcaggt
    26821 tttctcatgg cattaaccgc cgcgcttaaa gcgcaaattg ccgcctggta taaggcgctt
    26881 caggaacaga tccccgactt tattccccgt gcgccgcagc ggcagatgat tgcggacgtc
    26941 gccaaaacgc tggccggaga agaagggcgg catctggcga ttgaagcccc caccggcgtt
    27001 gggaaaacgc tctcctattt gatccccggc atcgccattg cccgcgaaga gcaaaaaacg
    27061 ctggtggtga gtaccgccaa cgtggcgttg caggatcaga tttacagcaa agatttaccg
    27121 ctgctgaaaa agatcattcc cgatcttaaa ttcactgccg ctttcgggcg tgggcgctac
    27181 gtttgtccac gtaatctgac ggcgctcgcc agcactgaac ccacgcaaca ggatctgctg
    27241 gcgtttcttg acgacgaact gacgccaaat aatcaggaag agcaaaaacg ttgtgcgaag
    27301 ctgaagggcg atctcgacac ttataaatgg gatggtctgc gcgatcatac ggatatcgct
    27361 atagatgatg atctctggcg tcgtttgagt accgacaaag ccagctgcct caaccgtaac
    27421 tgttactact atcgtgaatg cccgtttttt gttactcgtc gggagattca ggaagcggaa
    27481 gtggtggtgg caaaccatgc gctggtgatg gcggcgatgg aaagcgaagc cgtactgcct
    27541 gacccgaaaa atctgctgct ggtgctggac gaaggtcatc acctgccaga tgtggcccgg
    27601 gatgcgctgg agatgagcgc tgaaatcacc gcgccctggt atcgactaca gctggacttg
    27661 ttcacgaaac tggtcgctac ctgcatggag cagtttcgcc cgaagaccat cccaccgctg
    27721 gcgatccctg aacgtttgaa tgcgcattgt gaagagttgt atgagcttat cgcctcgtta
    27781 aacaacattc tcaatctcta catgcctgcc gggcaggagg cagagcaccg ttttgcgatg
    27841 ggcgaactgc ctgatgaatt gctggagatc tgccagcggc tggcaaaact caccgagatg
    27901 ctgcgtggtc tggcggagtt atttcttaac gatttaagtg agaaaaccgg cagccacgat
    27961 attgtgcgtc tgcatcggtt gattttacag atgaaccgcg cgctggggat gtttgaggtg
    28021 caaagcaaac tctggcggct ggcgtctctg gcgcaatctt ccggtgcgcc ggtgaccaaa
    28081 tgggcgacgc gggaagagcg cgaagggcag ctacacctct ggtttcactg cgtgggaata
    28141 cgtgtcagcg atcagctgga aaggctgctg tggcgcagta ttccgcacat tattgtcacc
    28201 tccgcaacct tgcgttcgct gaatagtttt tcgcgtttgc aggagatgag tggtctgaaa
    28261 gagaaagcgg gcgaccgttt tgtggcgctg gattccccct ttaaccactg cgaacagggc
    28321 aaaattgtta ttccccggat gcgctttgag ccttccatcg acaacgaaga gcaacacatt
    28381 gccgaaatgg cggccttttt ccgtgaacag gtggagagca aaaaatatct cggtatgttg
    28441 gtactgtttg ccagcggacg ggcgatgcag cgctttctcg actatgtgac ggatttacgc
    28501 ctgatgttgc tggtgcaggg cgatcagccg cgttatcgct tagttgaact gcaccgcaaa
    28561 cgcgttgcca acggtgagcg tagcgtgctg gtgggcttac agtcatttgc cgaagggctt
    28621 gatttgaaag gtgatctgct cagccaggtg catatccaca aaatcgcttt tccgcccatc
    28681 gacagcccgg tggtgatcac cgaaggggaa tggctgaaaa gcctcaaccg ctatccgttt
    28741 gaggtgcaaa gcctgccgag cgcctcgttt aacctgattc agcaggttgg gcgactgatt
    28801 cgaagccacg gttgctgggg cgaagtggtt atctacgaca aacgcttgct gacaaaaaat
    28861 tatggcaagc gactactgga tgcattaccg gtatttccga tagagcaacc ggaagtccct
    28921 gaaggtatag ttaaaaagaa agaaaaaacg aaatccccac gccgtcggcg gcgttaatga
    28981 tgtgagtcag gtaaggagtc gtaaatggac tatcgcaaaa tcattaaaga gatcgggcgc
    29041 gggaaaaacc acgcgcgcga tttagaccgg gatactgcgc gcggtctgta tgctcatatg
    29101 ctcaatggtg aagtccctga ccttgagttg ggcggcgtat tgattgcgct gcgtatcaaa
    29161 ggggaagggg aagcagagat gctcggcttt tacgaagcca tgcaaaatca caccatcaag
    29221 ctgacgccgc ctgcaggcaa gccgatgccg attgtcatcc ccagttacaa cggcgcgcgt
    29281 aaacaggcta acctgacgcc attattggcg attctgctgc ataaactcgg ttttcctgtg
    29341 gtggttcacg gagttagcga agatccaact cgcgtgctga cagaaaccat tttcgaactg
    29401 atgggtatta caccaacgtt gcacggcggg caggcgcagg cgaagctcga cgaacatcaa
    29461 ccggtgttta tgcctgtcgg cgcgttttgc ccgccgctgg aaaaacaact ggcgatgcgc
    29521 tggcggatgg gcgtgcgtaa cagtgcacat accctggcga aactggcgac gccatttgcc
    29581 gaaggtgagg cactgtgtct ttccagcgtt tcgcatccgg aatacattgg acgcgtcgcg
    29641 aagttcttta gcgatatcgg cgggtgggcg ttactaatgc atggtactga aggcgaagtg
    29701 tatgctaatc cgcagcgctg tccgcagatc aatctcattg accgtgaagg gatgcgggtg
    29761 ctgtatgaaa aacaggaccc tgctggtagc gagttactgc cacaagcaaa agatccggaa
    29821 accacggcgc agtggactga gcgttgcctg gctggcagcg aagcgattcc cgaatcgctg
    29881 aaaatccaga tggcttgctg cctggtggct acgggtgaag cggcaactat cagcgatggc
    29941 ctggcgcgcg ttaatcaggc attttaattt ttatctcagc cggatgagcc acgtcatccg
    30001 gcttttaccc caccgcaata tgaaattcct gcatctttat tgaccttccc gcgcccggcg
    30061 tgcagcataa aaaaacaaca aacacataac ataaacagga gttaaccatg gaaagtggtc
    30121 atcgctttga tgctcagacg ctgcacagtt ttattcaggc tgtatttcgt cagatgggta
    30181 gcgaggaaca agaagcgaaa ttagttgccg atcatttaat cgcggcaaac ctggcagggc
    30241 atgattctca tggtattggc atgatcccaa gctatgtgcg ttcctggagt caggggcatc
    30301 tgcaaattaa ccatcatgcc aaaatcgtta aagaggcggg ggcggcagtc acgctcgatg
    30361 gcgatcgcgc attcggtcag gtcgcggcac acgaagcgat ggcgctgggg attgagaaag
    30421 cgcatcagca cggtattgct gccgtggcgc tacataactc gcatcatatc ggccgtatcg
    30481 gttactgggc ggagcagtgt tcagcggcgg ggtttgtctc tatccacttt gttagcgtgg
    30541 tcggtattcc aatggtcgcg ccgttccacg gtcgcgacag ccgctttggc accaatccct
    30601 tctgtgtggt tttccctcgt aaagataatt tcccgctgtt gcttgattac gccaccagcg
    30661 ccattgcatt tggcaaaacc cgcgtcgcct ggcataaagg cgtccccgtg ccgccaggtt
    30721 gcctgattga cgttaacggc gtgccgacga ccaatccggc ggtaatgcag gagtcgccgt
    30781 tggggtcgct gttgaccttt gccgaacata aaggctacgc ccttgcagcg atgtgtgaaa
    30841 ttcttggcgg ggcgctttct ggcggtaaaa cgacgcatca ggaaacgttg caaaccagcc
    30901 ccgatgccat tcttaactgc atgaccacta tcatcatcaa cccggaactg ttcggcgcgc
    30961 cggattgtaa cgcgcagacc gaagcctttg ccgagtgggt gaaagcctcg ccgcatgatg
    31021 acgataagcc gattttgcta ccgggcgagt gggaagtgaa cacgcgtcgc gaacggcagg
    31081 agcaggggat tccattggat gcgggaagct ggcaggccat ttgtgatgcg gcgcggcaga
    31141 ttggtatgtc ggaagagacg ttgcaggctt tctgtcagca gttagccagc taaaaaaaag
    31201 cccgtccagt ggcggacggg caaacaaggg taacatagga tcaatgaggg ttagagcata
    31261 tgcgtctgtc ggcaaacaga cagggaaata cttgtgctgg acgtagcgta aacgcctgat
    31321 ccgtccaacg gttcggattt tgtaggcctg ataagacgcg gtagcgtcgc atcaggcatc
    31381 tgttgccgga tgcggcataa acgccttatc cgtcctacga attacttata aattaccgca
    31441 gtaccgctta atttattgtt gttggtggcg gaagtgatgc tgtatccact ggcaccggct
    31501 gccgccgctt tctcagccag tttcgcttcc agggcatcga gggtggatgc gccatcggca
    31561 gaaaccacgc cgattttatt catgttctgt gcctgggatg ccgttaccgg ttccgccgcg
    31621 aacacgccaa atgacagagt tgaaagagcc atagcagcaa caacagtatt gatagttttc
    31681 ataattaatc tctcgcaggt gatttttgta taagtgacgt tgtttcgtcg atgtgatgag
    31741 tatcacgttt ttttacgaga gataaaatcg aagagaattg accgccttgt tcaaataaat
    31801 tgattgatat ctaacatact gataaaccat ttatttgttg tacttatgga cttgttttat
    31861 gccgcgtggc agtcacagca gcgttaaagg cacggaatgc cacgcaaaat acaaattata
    31921 ctcgctatgc atctcgccag ttgtagttaa cgtaactgtc tgtctttcga acctctgcga
    31981 ttatatcctg tatacgctgg tttttgtaaa tctttctcct gctgacaatg aatacataag
    32041 cgcacgccag gaatggcttc ccgacgggct tgcgggatgg gggcaccgca ctcttcacat
    32101 tcatacaggc tttcgccacg cggaatttca ccccgagcgc gggcaatcgc atcttcaatt
    32161 gtactgttga tctgttcgtt gacggcgtca tcgttagccc aagcggatgc catatcgtcc
    32221 tccccatatc aatgcttgta cagttaagtg tagctaatat agggacgaac ctgggcagtt
    32281 caagcgtcag atctccgacc attcccgcag cagattatga taaagattga gcagcgacag
    32341 gatctcttca ttttcaccat agcggctttt cagcgactga atatttttgt ccagttcaaa
    32401 cagcatggcg cgctttttat catcgcggat catcgactgg atccacataa atgatgccac
    32461 tcgtacgccg cgggttacgg gtgtcacgca atgcaggctg ctggagggat acaacaccag
    32521 atcacctgcc gggagtttta cccgatgttg tccaaaggtg tcattaacga ccagttcgcc
    32581 gccgtcgtag ctttgtggat cgcttaaaaa cagcgtggca gaaaggtcag tgcgcatcca
    32641 gccgttttgt ggatggctgc gtactgcgcc atccacatgg aaaccatagg tttcattgtt
    32701 ctgatagcga ttaaacagcg gcgtggaaag ggtacgcggc aaggcagcgg caaagaacaa
    32761 tgcatgttgg ttaaccgcgt tcagcacctc attttgcagt gcggcgtata acgcgctgcg
    32821 ggtgtctact tgctgattgt tcttaacttg tgcaccttgt gctccggtgg tgacgcgtcc
    32881 atccacccat tcggcttgtt ccagttgttc gcgaaaatgg gcgacgtcct gtggcgataa
    32941 cacgccggga atgtggtaca tcatacaagt tctccaaaaa gtggggcctg cgccccacat
    33001 ctgaatcaga aatgcatatt ggctgtgagc aagaaggttc ttggctcgcc cgggtgataa
    33061 cggtagccgc tcttgttgat tgaggcgacg taatcggtat caaacaggtt gtaaacgttt
    33121 agctggaagt cgagattgcg attaacccga taccccagtt tggcatcggc gacccagtaa
    33181 ccttctgtaa atgctggcgt tcccaccgcg ccgtctgaac ctttatgtat actgccgata
    33241 tagcgtgcgc ccgcgccaac agagatatca tcggttgcct gatattggct ccataaggtg
    33301 aaggcgtgct ccggggtata cggcagcgat gaggaaccat cctgggcaac atctttgccg
    33361 tttttgatgg ttgctttttg ctgggtatag ccgccaatca cctgccacgc gggagtgata
    33421 ttccctgcca cggatatctc atagccttcg acgcgtttct taccgtattg cgagtaagtc
    33481 ccgtcatcat tttgctcaac ttcattttcg atatcagtac ggaacagcgc ggcggtgagc
    33541 aacaggcgtt tatccagaac ctgccatttg gtgccaatct cgctggtgtt ggctttttgc
    33601 ggtttaaaat cggtgcggtt ggcactgtta ccactgccag actgcgcaag ggcgaagttg
    33661 ttgccgcccg gaggctgctg ggaaacggca tagttaatat agacattgcc gttttccgtc
    33721 aggtggtaca gcgccccggc tttccagttc accagattgc ccgacttggc ggtgtcgacg
    33781 gtggtgactg gagaaccttt tgccacacca gctgggcagg tgatggcacc gcgtccgctg
    33841 ccgccgcagg cggtggcact gtcatattca gtatgataat tatccagacg gatcccgccg
    33901 ttcagctcaa aatcacgggt gatttgcagc gtatcgaagg cgtaaattgc gaaggtatcc
    33961 gtctgaccat tggcgtttgc gccgttgcgc gtcaggccgc cgggatgaat tctgctgtca
    34021 ggatgataaa tatttaccgc cggtaacgtc accggattaa cgccatagtt agtctgcgtt
    34081 tcacgggtaa attccacgcc ggtactgaca tcatgaccga tagaaccggt atagaacgtc
    34141 gaggtcaggt tggtctggtt ggtgagaatt ttattactca catctttggt attcgccgtg
    34201 cgtgactagg tccagctatt cacatcgctg gtgggctgag taatattcga cgccccgccc
    34261 ataatcgccg tcatcaggta atcctgcttt acgcgcgacc aacgggtagt attgcgaatg
    34321 gtggtgttat cgttgatgtc gtgctcaaaa cgcatggtgg cggtgtcggt ggtcgaatcg
    34381 tcgtaatcgg aatccgtgcc gtaaaagtta tgagtatcaa cttttccgga atgattcagg
    34441 gccgccgctc ccgcagatgg ggcagaatag cccggcaaac cgatggtcgg aatgccgccg
    34501 tctggcgtgt tgtgctgagt gacatgcaga taattaagat acaaacgatt tgctgtacca
    34561 aggccaaaag cgacagaagg ggcgacgccg taacgctcat ttttgacttt gtcgcgtccg
    34621 gcatcgtggg ttttttcgcc catcacattc aggcgcaccg cggtagtatc accaatgacc
    34681 tgattgacgt ccagcgtgcc gcggcggaac caggcgctgc caatactggc ggaggcgtca
    34741 atgccggaat cattgcgcgg ctgcttgctg atcatattga tcgagcctgt cggtgcgctg
    34801 cgcccgtagt cggtgccgga cggcccttta atcacttcga cctgctcggt attgaaggtg
    34861 tcgcgcgaga cgctgccgat atcgcgaata ccatcaatat aaatactgtt agaggtatcg
    34921 gcaccacgca tataaatggc gtcgccagtg gtggagttac cgttctcacc cgcaaaaaac
    34981 gcacccacgc cgg
//
LOCUS       DAEEGA010000036        34937 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEEGA010000036 DAEEGA010000000
VERSION     DAEEGA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34937
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.036"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..2261
                     /locus_tag="KLH46_001912"
     CDS             <1..2261
                     /locus_tag="KLH46_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HBE0378610.1"
                     /translation="AKSLYLNPDKNTVNAFIHQQDINVLAQDDYQSLSIQGNGALGIT
                     ENSYIGAHWNFNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNAQGGN
                     FTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLGSFYL
                     NSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKTT
                     SQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNLAISA
                     SVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISAT
                     VSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRAI
                     VMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRYS
                     EQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSD
                     SYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQD
                     LQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNREL
                     MLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGS
                     EKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKLR
                     GNVQTAQREVAP"
     gene            2258..3346
                     /locus_tag="KLH46_001913"
     CDS             2258..3346
                     /locus_tag="KLH46_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HBE0378611.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3434..4732)
                     /gene="exuT"
                     /locus_tag="KLH46_001914"
     CDS             complement(3434..4732)
                     /gene="exuT"
                     /locus_tag="KLH46_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HBE0378612.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5216..6628
                     /gene="uxaC"
                     /locus_tag="KLH46_001915"
     CDS             5216..6628
                     /gene="uxaC"
                     /locus_tag="KLH46_001915"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBE0378613.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6643..8130
                     /gene="uxaA"
                     /locus_tag="KLH46_001916"
     CDS             6643..8130
                     /gene="uxaA"
                     /locus_tag="KLH46_001916"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBE0378614.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8213..8764
                     /locus_tag="KLH46_001917"
     CDS             8213..8764
                     /locus_tag="KLH46_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBE0378615.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8769..10013)
                     /gene="sstT"
                     /locus_tag="KLH46_001918"
     CDS             complement(8769..10013)
                     /gene="sstT"
                     /locus_tag="KLH46_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBE0378616.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10609..11574)
                     /gene="alx"
                     /locus_tag="KLH46_001919"
     CDS             complement(10609..11574)
                     /gene="alx"
                     /locus_tag="KLH46_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBE0378617.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11845..12843)
                     /locus_tag="KLH46_001920"
     CDS             complement(11845..12843)
                     /locus_tag="KLH46_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE0378618.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12922..13614)
                     /locus_tag="KLH46_001921"
     CDS             complement(12922..13614)
                     /locus_tag="KLH46_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBE0378619.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13687..14190)
                     /locus_tag="KLH46_001922"
     CDS             complement(13687..14190)
                     /locus_tag="KLH46_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBE0378620.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14275..15411
                     /gene="rlmG"
                     /locus_tag="KLH46_001923"
     CDS             14275..15411
                     /gene="rlmG"
                     /locus_tag="KLH46_001923"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBE0378621.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15696..16010
                     /gene="higB"
                     /locus_tag="KLH46_001924"
     CDS             15696..16010
                     /gene="higB"
                     /locus_tag="KLH46_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HBE0378622.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            16007..16423
                     /gene="higA"
                     /locus_tag="KLH46_001925"
     CDS             16007..16423
                     /gene="higA"
                     /locus_tag="KLH46_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBE0378623.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16468..18486)
                     /gene="fadH"
                     /locus_tag="KLH46_001926"
     CDS             complement(16468..18486)
                     /gene="fadH"
                     /locus_tag="KLH46_001926"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBE0378624.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18712..21063)
                     /gene="ygjK"
                     /locus_tag="KLH46_001927"
     CDS             complement(18712..21063)
                     /gene="ygjK"
                     /locus_tag="KLH46_001927"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBE0378625.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(21080..21499)
                     /locus_tag="KLH46_001928"
     CDS             complement(21080..21499)
                     /locus_tag="KLH46_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378626.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21477..21830)
                     /locus_tag="KLH46_001929"
     CDS             complement(21477..21830)
                     /locus_tag="KLH46_001929"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378627.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21831..22151)
                     /locus_tag="KLH46_001930"
     CDS             complement(21831..22151)
                     /locus_tag="KLH46_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378628.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22285..23718)
                     /locus_tag="KLH46_001931"
     CDS             complement(22285..23718)
                     /locus_tag="KLH46_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE0378629.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23781..24230)
                     /locus_tag="KLH46_001932"
     CDS             complement(23781..24230)
                     /locus_tag="KLH46_001932"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBE0378630.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24227..27304)
                     /gene="ebgA"
                     /locus_tag="KLH46_001933"
     CDS             complement(24227..27304)
                     /gene="ebgA"
                     /locus_tag="KLH46_001933"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBE0378631.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLDDYT
                     LHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTRYSE
                     AGHSIASYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSGKPT
                     SCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSDGEV
                     LIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMGING
                     EYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTALTN
                     RHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSNSWG
                     SEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27487..28470)
                     /gene="ebgR"
                     /locus_tag="KLH46_001934"
     CDS             complement(27487..28470)
                     /gene="ebgR"
                     /locus_tag="KLH46_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBE0378632.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28688..29019
                     /locus_tag="KLH46_001935"
                     /pseudo
     CDS             28688..29019
                     /locus_tag="KLH46_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(29061..30440)
                     /gene="ygjG"
                     /locus_tag="KLH46_001936"
     CDS             complement(29061..30440)
                     /gene="ygjG"
                     /locus_tag="KLH46_001936"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBE0378633.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30858..32378
                     /gene="aer"
                     /locus_tag="KLH46_001937"
                     /pseudo
     CDS             30858..32378
                     /gene="aer"
                     /locus_tag="KLH46_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32532..33155)
                     /gene="nfeR"
                     /locus_tag="KLH46_001938"
                     /gene_synonym="yqjI"
     CDS             complement(32532..33155)
                     /gene="nfeR"
                     /locus_tag="KLH46_001938"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBE0378634.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33432..34177
                     /gene="nfeF"
                     /locus_tag="KLH46_001939"
                     /pseudo
     CDS             33432..34177
                     /gene="nfeF"
                     /locus_tag="KLH46_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34231..34306)
                     /locus_tag="KLH46_001940"
     tRNA            complement(34231..34306)
                     /locus_tag="KLH46_001940"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34271..34273),aa:Ile,seq:cat)
     gene            34431..34937
                     /gene="mug"
                     /locus_tag="KLH46_001941"
                     /pseudo
     CDS             34431..34937
                     /gene="mug"
                     /locus_tag="KLH46_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8537 a         8974 c         9176 g         8250 t
ORIGIN      
        1 atgcgaagtc attatattta aatccagaca aaaatactgt taacgctttt atacatcagc
       61 aagacatcaa tgttctggca caggatgatt accaatcgtt gtctattcag ggaaacggtg
      121 cgctgggaat aacagaaaat agctatattg gtgcacactg gaatttcaac ggttatgatg
      181 cagatgatgt cagtgacagt aatactgacg tcagcgatct ctattatcgt tatgattttt
      241 tacgtcgtta ttatgtgcag gcgggtcgca tggacaaccg cacactattt aatgcacaag
      301 gcgggaactt tacctttaac tttctgccac tcggtgcaat cgatgggatg cgtatcgggt
      361 cgacactgag ctatttaaac caggcgcaaa gccagcaagg aaccccagta atggttctgc
      421 tttcgcgcaa ttctcgtgtt gacgcttatc gtaatgagca acttttggga tcgttttatc
      481 tcaatagtgg ttcgcaattt attgatacca gttcctttcc gccaggcagc tacagcgtgg
      541 cattaaaagt ctacgaaaat aaccaactca cccgcaccga gcttgtgccg tttaccaaaa
      601 ccggcggtct gactgacgga aatgcgcaat ggttcttaca ggcaggtaaa actacatcac
      661 aggtttctga tgatgaaagt tcagcttatc agctaggagt acgcctgcca ttacatccgc
      721 aatatgagct ctacgcaggg ctggcgaatg ccgatgatgt gagtgctttc gagttaggta
      781 ataactggac ggcagattta ggcagggtgg ggaatcttgc aatcagcgcc agcgtgttcc
      841 gtaacgatga cggcggcaaa ggtgatatgc aacaggccaa ctggagtaat ccgggatggc
      901 cgacgttggg cttttatcgg accaactctg acggcgatgc ttgtacaacc gacagcagag
      961 agagctataa cgccttaagc tgttatgaaa gtatttccgc gacagtttca ctgaattttg
     1021 tcggctggaa tatgatgctg ggttatacct gcacacaaaa taacactgat gatagtttgc
     1081 gttgggataa acagcagagc tttgaaaata actatcttcg ccagacaaca gcgcaaagta
     1141 tctctgaaac tgtacaactt agcgcttccc gcgctattgt gatgcgtgac tggattttga
     1201 gtacgtcagt cggtgttttc catcgtaatg acaacggtgg cgataacgac gacaacggct
     1261 tgtacttatc gttttcgtta tctgacacgc caacgatgga tagcaataac aacagccatt
     1321 caaccaatgt gcctacggat tatcgttata gcgaacagga tggcgatcaa acgtcatggc
     1381 agttatcgca taccttttat aacgattcat tcagccataa agaacttggc gtaaccgtcg
     1441 gaggcctgaa caccgatacc ataaacagcg cggttaacgg gcgttgggat gggcaatacg
     1501 gaaatgtcta cgctaccgta tctgatagtt atgaccgtaa gaatcatgat catctttcgg
     1561 cctttacggg cacttatagc tctacgctgg ctgtcagtcg ctatggcgtt aatttgggag
     1621 ccagtggtac agacgatttg ctgggtgcgg tactggtgga tgtgaaaggc ttctctgaac
     1681 aggatgaaga gagtcaggat ctgcaactcg aagcgcgggt ggcaggcagc cgaactttgc
     1741 agcttggtca aagtgacagt gtgttgttcc cttatcctgg atttcagtct ggttttgttg
     1801 aggttaacga cagtagccag ggcaatcagc aggggacaac aaacatcatt aacggtgcag
     1861 ggaatcgtga attaatgttg ttgcctggca agctgcgcta tcgcgaagtg tctgccagct
     1921 ttaactacaa ctatatcggt cgtttattac tgccggcagc ggtgaaaaaa ttcccgattg
     1981 tggggctgaa cagcgccatg ttactggtag ctgaagatgg cggatttaca cttgaaatta
     2041 acggcagcga aaaagagctc tatctgcttt ccgggcagca attccttaag tgtccactga
     2101 gtgttgtaaa gaaacgcgcc agcattcgtt acagcggaga tgttacttgt agtgtggtga
     2161 cttattcaca attaccggag tcgattcagg ttcaggcaca gttgaaacag cctaaattac
     2221 gtggaaacgt tcagacggcg caaagggagg ttgcaccatg agaaatcgat tgattgcggc
     2281 gatattgggc ttgtttggca cgctcactgg cgttcaggca gctcctgacg tgaccagtga
     2341 aattacgtat gatttggcat ctggtagagc ggattattac ttctggaagg atgaagcgtc
     2401 ggcaggaaat aatggatata tgtggtatga atgttcgtat cctgacctcc aacaaacctg
     2461 tacagctaat ggaaatatat cgacagtaca aatctattta actgaacaac gcagtgggat
     2521 gcgttggccg gtaaaactca aaggatttaa aacagccatt gtaagtagcg atgaagcgcc
     2581 gctaggatgc aaggggggca aagggcttca gacgaatctt aaggattcta atagatcttc
     2641 atgtacagaa gatggtcaac attattatat atacgataca aagtttctta cgctctacct
     2701 tgagcagaca gagatgaaga atttgccgat tggtggcgtc tggaagggga aagttaaatt
     2761 acattcgaac agcccggccc aggactattt cgcaaatatt accctgaata cgctcgaccc
     2821 caaccatatt gacgtgttct tcccggagtt cgcccacgcc acgccaaggg tgcagttaga
     2881 cttgcatcca acaggaagcg ttaacggcag caactacgcg caagatctga ccatgttgga
     2941 catgtgcctg tacgatggtt ttaacggtaa tgccatcagt tatgaaatca tgctcaaaga
     3001 tgaagggcga ccagctgcag ggcgcagaga cggttacttc tctatctatc gtcagggagg
     3061 gaccaccacc gacgagggag aacgcattga ttaccgggtc aaaatgtaca acccggaaac
     3121 cggtgggcaa attgatgtgc gcaataatga aaatatggtc tggaacagca ttaacctgaa
     3181 acgtgtgcgt ccggtggtac tgcccggtat tcgctatgcc gtgatgtgtg tgccgacacc
     3241 cttgactctg gcagtcgata aattcagcgt gatggataaa caggccgggt actacatggg
     3301 caaattgtca gtaatcttta cgccttcctt gccaaccatc aattaaccct gcaaaccgga
     3361 caaacgcttt tttctatata aacgacgaag ccgcctgata aggcggcttc gtcgttataa
     3421 cttacgttaa acgttaatgt tgcggtgcgg gatcgttatg tgtttcctgc gccacctcaa
     3481 tcgccggttt gttctgcaat acggtccaga taaccagcgc acccagcagg tcaaatactg
     3541 ccagaactgc gaacagcggg ctgaagccga tggtgtcagc cagtgcaccg acaaccagcg
     3601 caaacagcgt acttgccagc catgcggaca tcccggttaa accgttcgcc gtcgccactt
     3661 cgttacgacc gaacacgtca gaagagagcg taatcagcgc gccagacagt gcctggtggg
     3721 caaaaccacc gatacacagc agcataattg cgacatacgg gttggtgaac agaccgatca
     3781 tacccgggcc aatcatcagc acagcaccca gcgttacgac catcttacgg gaaacgatca
     3841 ggttcacacc aaaccaacgc tggaacagcg gcggcaggta accaccgagg atacaaccga
     3901 ggtcagcaaa cagcatcggc atccaggcga acatcgcgat ctctttcagg ttaaagccgt
     3961 aaactttaaa catgaacagc gggatccacg cgttaaaagt accccaggcc ggttctgcaa
     4021 ggaaacgcgg cagtgcgata ccccagaact gacggttacg caggatctga ccaacggaca
     4081 ttttcttcgc cgtgctcacc tggtgctggg cttcctgacc attaataata tagtcgcgtt
     4141 cttcatcggt cagatgcttc tggtcgcgcg gatgtttata gaaaatcagc catgccatcg
     4201 cccagataaa gctcaatgca ccggagatga taaatgccat ctgccagctg tgcatcacga
     4261 ttgcccatac caccagcggc ggcgcaatca tcgcaccaat cgaagaacct acgttaaagt
     4321 agcctactgc gatggaacgc tctttcgccg ggaaccattc ggagctggct ttcagacccg
     4381 ccgggatcat cgctgcttcc gcggcaccga ccgcaccacg agcaacagct aggccacccc
     4441 agctacctgc cagcgcggtt gcaccacaga acacggccca cagcacagca aacattgcat
     4501 aaccgatttt cgtacccagc acatccagta cataacctgc taccggttgc atgaccgtat
     4561 aagcagcaga ataggctgcg atgatatagg aatactgttg ggtggagatg tttaactctt
     4621 ccatcagagt tggcgcagct gccgccacag tgttacgcgt caggtaacca agcacggtgc
     4681 cgagcgtcac cagtgcgatc atataccaac gtaacccttt aattttacgc atgtaaaacc
     4741 tcatcgtttt gttatatccg caggaagcgc ggccatcatc ccgccagagg ctgaagatgt
     4801 tagttacgac cgggtaaccg aaaaaaaaac cggcacgccc cgaacgttgc catgaaagtt
     4861 tcgtgctaat tcggcttccg taccggttaa cccgattcat ctaacaaatg aatgaatcgg
     4921 caatttgcat tccgtcgact tatgatttgc gacggcagaa agataacttg tcatacaacc
     4981 ttaaaaggtg agagccatca caaatgtgcg aatatttgta gggacattac ctgacgacag
     5041 cgaggccagt actggcgcgg cctgcagcga gatttaccac tttgagagta atttttttaa
     5101 ctacgtttat tgatctaact caagaaaata tcttcggact ctggaaattg gtgtgataac
     5161 tttgtcagca tcgcaccata agcaagctag ctcactcgtt gagaggaaga cgaaaatgac
     5221 tccgtttatg actgaagatt tcctgttaga taccgaattt gcccgccgtc tgtatcacga
     5281 ctacgcaaaa gaccagccga ttttcgatta ccattgccat ttgccgccgc agcagattgc
     5341 ggaagactat cgttttaaaa acctgtatga catctggttg aagggcgatc actacaaatg
     5401 gcgcgctatg cgtaccaacg gtgtggctga gcgtctgtgt actggtgatg cgtctgaccg
     5461 tgaaaaattt gacgcctggg cggcgactgt tccgcacact atcggcaacc cgttatacca
     5521 ctggacgcac ctcgaactgc gtcgtccgtt cggtatcact ggcaaattgc tttctccgtc
     5581 aactgccgat gaaatctgga acgaatgtaa cgaactgctg gcgcaggata acttctccgc
     5641 gcgcggcatc atgcagcaga tgaacgtgaa aatggtcggc accaccgatg acccgatcga
     5701 ttctctggag catcacgcag agatcgccaa agacggctct ttcaccatca aagtgctgcc
     5761 gagctggcgt ccggacaaag ccttcaatat cgaacaggcg acctttaacg actacatggc
     5821 gaagctgggc gaagtttccg ataccgacat tcgccgcttt gctgacctgc aaactgcctt
     5881 gaccaaacgt ctggatcact tcgccgctca cggctgtaaa gtgtctgacc atgcgctgga
     5941 cgtggtgatg tttgctgaag cgaacgaagc ggaactggac agcattctgg cgcgccgtct
     6001 ggctggcgaa accctgagcg agcacgaagt ggcacagttc aaaactgcgg tactggtgtt
     6061 ccttggtgcc gaatatgcac gtcgcggctg ggtacagcag taccacattg gcgcgctgcg
     6121 taataacaac ctgcgtcagt tcaaactgct ggggccggat gtaggctttg actccatcaa
     6181 cgaccgtccg atggcggaag agttgtctaa gctgctgagc aagcagaacg aagaaaacct
     6241 gctgccgaaa accattctct actgcctgaa cccgcgcgat aacgaagtgc tgggcaccat
     6301 gatcggtaac ttccagggcg aaggtatgcc gggcaaaatg cagttcggtt ccggctggtg
     6361 gtttaacgat cagaaagacg gtatggaacg tcagatgacc caactggcgc agctcggtct
     6421 gctgagccgc tttgtcggta tgctgactga cagccgtagc ttcctgtcat acacccgtca
     6481 cgaatacttc cgccgcattc tgtgccagat gatcggtcgc tgggtggaag cgggcgaagc
     6541 accggcggac atcaacctgc tgggcgagat ggtgaaaaat atttgcttta acaatgcgcg
     6601 tgactacttc gccattgaac tgaactaagg tctggggttg atatgcaata catcaagatc
     6661 catgcgctgg ataacgtcgc ggtcgcttta gcagatttgg ctgaaggcac agaagtcagt
     6721 gtcgataacc agactgttac gctgcgccag gatgttgctc gtggacataa atttgcgtta
     6781 acggatatcg caaaaggggc caacgtcatc aaatatggcc tgccgattgg ttatgcattg
     6841 gcggatattg cggcggggga acacgtccac gcccacaata cgcgcacgaa tctgagcgat
     6901 ctggatcagt atcgctatca acctgatttt caggatctgc ctgcgcaagc ggcagatcct
     6961 gaagtgcaga tctatcgtcg cgctaacggc gatgtcgggg tgcgtaacga gctgtggatc
     7021 ctgccaaccg tgggctgtgt caacggcatc gcgcggcaaa tccagaaccg tttcctgaaa
     7081 gagaccaaca acgccgaagg taccgacggc gtgttcctct tcagccacac ctatggctgc
     7141 tcacaactgg gcgacgatca catcaacacc cgcaccatgc tgcaaaacat ggtgcgccac
     7201 ccgaacgcgg gcgcagtgct ggtgattggt ctgggctgtg aaaacaacca ggttgccgca
     7261 ttccgtgaaa cgttgggcga tatcgatcct gaacgcgttc atttcatgat ctgccaacag
     7321 caggatgatg agatcgaagc cgggatcgag catttgcatc agctgtataa cgtgatgcgc
     7381 aacgataaac gcgagccagg caaactcagc gaactgaagt ttggtctgga gtgcggtggt
     7441 tctgacggtc tttctggtat tactgctaac ccgatgctgg ggcgtttctc tgactacgtg
     7501 attgctaacg gcggtactac cgtactgacc gaagtgccgg agatgtttgg cgcagagcag
     7561 ttgctgatgg accattgccg cgacgaagca acgtttgaaa aactggtcac catggtcaac
     7621 gacttcaaac agtactttat tgcccatgat cagccgatct acgaaaaccc atcaccgggg
     7681 aacaaagcgg gcggtatcac cacgctggaa gacaaatcac ttggctgtac ccagaaagcg
     7741 ggttccagtg tcgtggttga cgtgctgcgt tacggcgagc gtctgaaaac gccagggctg
     7801 aacttgttaa gtgcgccggg taacgatgcc gtagcgacca gcgccctggc gggtgcgggc
     7861 tgccatatgg tgctgttcag tactggtcgc ggcacgccat acggtggatt tgtgccgacg
     7921 gtgaaaatcg ccaccaacag tgaactggcg gcgaagaaaa aacactggat cgactttgac
     7981 gcgggtcagc tgatccacgg taaagcgatg ccgcagttgc tggaagaatt tatcgacacc
     8041 atcgttgagt ttgccaacgg taagcaaacc tgcaacgagc gtaacgactt ccgcgaactg
     8101 gcgatcttca aaagcggcgt aacgctataa agtcgaaatg agttgttaat aaaaaacggc
     8161 gtttcataat gtgaagcgcc gtttgctttt ccatacctgc taaggattta tcatgaccgc
     8221 gtattggctg gcccagggcg tgggtgtcat cgcctttctg attggtatca caacattttt
     8281 caatcgtgac gaacgtcgct tcaaaaagca gctttcggtc tatagcgccg ttattggcgt
     8341 acattttttt cttctgggca cctatcccgc tggtgccagt gccatcctta atgccattcg
     8401 tacattgatt accttacgca cgcgcagctt atgggtaatg gcgattttta ttgtgctgac
     8461 tggcggaatt ggcctcgcga agttccatca tcctgtcgaa ctattgccgg ttatcggtac
     8521 gattgtcagt acctgggcgc tgttccgctg taaagggctc accatgcgct gcgtgatgtg
     8581 gttttcaacg tgttgctggg tgattcacaa cttctgggcg gggtcgatag gcggcacgat
     8641 gattgagggt agttttctgc ttatgaatgg cctgaatatc attcgtttct ggcggatgca
     8701 gaaaagggga attgatccgt ttaaagtaga gaaaacccct cccgccatag acgaaagggg
     8761 ttaaacaatt aattacgcag ggcgctattt gccagacgat cgtcttccgc ctggcaagct
     8821 gccgcagtga acagcacgtc agttgaagag ttcagcgcgg tttcgcaaga gtcctgcaat
     8881 acgccgataa taaagccgac ggcaaccacc tgcatggcga tatcgttcga aataccgaac
     8941 atattacagg ccagtgggat cagcagcaaa gaaccccccg ccacgccgga tgcgccacag
     9001 gcacacagag aagccactac gctcaacagc agcgctgtgg gcagatcgac cggaataccc
     9061 agcgtattaa ccgcagccag cgtcaacacg gtaatggtga ttgctgcgcc cgccatattg
     9121 atggtggctc ccagcggaat agaaactgaa taggtatcgc gatccagatt cagcttttca
     9181 cacagcgcca tattcaccgg aatgttcgct gcagagctgc gggtgaagaa ggcatacaca
     9241 ccgctttcgc gcaggcacag cagcaccagc gggaacgggt tacgacgaat tttccaccac
     9301 accagcaatg ggttaaccac cagcgccacc agtaacatac agccaaccag cacgaccagc
     9361 agttgcgcgt agccccacag tgtggagaaa ccggtggttg ccagggtaga agaaaccagc
     9421 ccaaaaatac cgaacggtgc gaagcgaatg accagtttca ccataaaggt aacggcattc
     9481 gacatatcat tgaccaggtt tttggtggtc tcgttaccgt gacgcagtgc gaagccgagg
     9541 ccgatcgccc acaccagaat cccgatgtag ttacctttca gcagggcgtc gatggggttg
     9601 gaaaccatgc tcattaccag tccgcgcatc acttcgacaa tgcctgacgg cggcgaaata
     9661 tcacccgcgc tactggacaa gtgcagggta gaagggaagg caaagctgaa gactactgcg
     9721 gccagagctg cagagaaggt gcccagcaga tagaggaaca aaataggacg gatattggtt
     9781 ttctgcccgt gctggtggtt agcaatagat gccattacca gcatcaacac caggatgggg
     9841 gcaacagctt tcagtgcgcc gacgaacaaa gtacctaaca gaccaacagc ttccgccgcg
     9901 ggttttgaga tccatgccag aagaatcccc agaacaaggc cgaccaggat ttgttttacc
     9961 aggctgccat gagccagacg ccggaatagc cccggtgaac gttgcgtagt catttttcga
    10021 tcctttcatt gtgttgtcgc acatccctgg tgcgttctgt cgacgcatct ttcaggatgt
    10081 aacaaagtgt ttgcaaggtc gagtataagg aaagttacag cttcgggaag cgaaaaatgc
    10141 tggattttaa agagaggtca tgttttgcaa cttaatggtt gcatttattt aacataaaca
    10201 gagggaagga ctggctacta aggctgatta gataacatat gggcaaaact atttggaata
    10261 tgagaacggt tgcgctcctt taacgtgtta tgccatataa agtcactggc gcagggagcg
    10321 cgcagggggc ggccaatcgc cgccgccccc tgcaccccca ggctctggcg aacaaaatcg
    10381 ccgctgcgcg gttccctcag cctatccctt gcggctaccg ggtcgggcgc aaggtaacgt
    10441 ccctgtaaga tgcgccctcg ccccggcctc cagggaacgc ttcggcgatt ttgacgccac
    10501 caacacccgt gcggtctttt tgtaaagagc tataagccga ttacataatt aatagctcac
    10561 ctttgtctgg aatgagttat ctctaggaat ggcttaatat acagcgaatc attctccccg
    10621 ctgcttatca tgccgataat tcacccaggc gttgataata aacgtcatca ccagaatgcc
    10681 aaacaccacg cccagcgaga cggcgattgg aatatggtag aagtcgacaa tcagcatctt
    10741 gataccgata aacaccagaa tcaccgccag gccatatttg agcatcgaga aacgctctgc
    10801 tacgcccgcc agcaggaaat acatcgcacg caggccgagg attgcaaaca ggtttgaggt
    10861 cagcacaatg aacgggtcag tggtcacggc gaagatagcc ggaatgctat ccacggcaaa
    10921 aatcacgtcg ctcaattcca ccagaatcag taccagcatc agcggtgtgg cgtacagcaa
    10981 tccattctta cgcacaaaga aatgctcgtt gtcgatggtg tcggtcatgc gcaaatgacc
    11041 gcgtagccag cgcaccagcg gcttgtcgcc aatgcctgat tcatcttcat gggcgagcgc
    11101 catcttcacg ccggtaaaca gcaggaaggc accgaagata tacaggatcc agtcgaactg
    11161 cgaaatcagc cagctgcctg tgaagatcat gatggtacgc agaacaatcg ccccgagcac
    11221 gccatacacc agcacgcgac gttgtaatgc cgccggaacg gagaaatagc tgaacaacat
    11281 cagccagaca aagacgttat cgaccgccag cgatttctca atcagataac ccgtgagaaa
    11341 ggccagggcc tgtggatcgg caacggcgcg accttcggtt tgcaccagat accaccagaa
    11401 agcggcgtta aacagtaacg acagcgtcac ccagaccagc gaccaggccg cagcctgttt
    11461 catggtcatg gcatgtgccc cacgacgccc ctgcaacaac aggtcgatag ccagcataat
    11521 ggcgacaaca acagcgaatc cgccccatag caacggcgtg ccgacagtat tcatagaagt
    11581 tccttacaca taaaaaaacg gccaacgtcg gaagacgtca gccgctgctt tttatgcata
    11641 gacctcgcct tccggcaagg tctcacttac aacaaaaaag gaatacgtct gcgtattcct
    11701 ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc gtaatgacga tcgaccggca
    11761 atgaagttac tcccctttgc aggtaacaaa gtatgacaga ccattcagtc agtcaatggt
    11821 ctgttacata ccttttacac tgttttactt tggcaagtat agttttacgc tatctgccgg
    11881 aaaaatcacc ccagtttggc ggcggatctc ggtcagcagt ttggctgtga tgcgactgac
    11941 cgccagccct ggatgatcca ccagatgctc atccaccagg gtagcgaaca gctctgcttc
    12001 gtagagcatg gtattaatat gctgcggctg ggtgagatcc tgcatctggc tgccacgcgg
    12061 cacaaagcag actttctggc attcagacag tttttcaatc accagcgatc ccgcttcgcc
    12121 ctgaatctcg cttcccagga cagaatcact gacctttgag tgctgcaagg tgacgctgaa
    12181 atcaccgtaa tccatcacca ccacgccgtg ggcgtcaacg ccgcttgcca gcaaactcgc
    12241 cgttgcctgc acgcttttcg gctcaccaaa taacgccacc gccgacgcca ggcagtaaaa
    12301 gccgatatcc ataattgaac cgttagagaa tgccggatta aaggtgttgg gattctcacc
    12361 gtccaggtaa cgttgatacc gcgaggaata ctggcaatag ttgaaaaaga ctttacgcag
    12421 tttgcctgct ttcggcagcg cctggcgcaa caaatgaaag ttcggcaggc aggcggtttt
    12481 aaatgcctca aacagcacca cctgattttc gcgcgcacag gcaatggcgg catccacttc
    12541 cgccagattc gacgccagcg gtttttcgca aatcacatga attttatggc taaggaaaag
    12601 ttgtgtctgg gaaaagtgca gggaattcgg gctggcaata tacaccgcgt caatggcatc
    12661 gctttccgcc atcgcttcca gcgacgtaaa cagatgctcg acagaaaaat cattggcgaa
    12721 gtgctgagcc tgctcaaggc tgcgggaata tacggcggtt aacttgtatt taccgctctc
    12781 atgggcggcc tcgacgaact ggcgagtgat ccagttcgta ccaatcacag cgaaacgtat
    12841 cataacgcgt ggatactcca taaagggatt ctgtcgtcaa tgtagcacgc ctttttcatc
    12901 ttcgacgtgc gcagatacgc attattcttt cgccggacag ccatgctcac taaacggccc
    12961 aatcatgacc gatggcccga gatatttcgg ctggcgatgg aggatttttg cgtcaatcac
    13021 cgcgctgaca cgagcgagtt tctcccgcag ttgagtataa aaaccgtgct tcatattgag
    13081 gtctggcgcg ttaaacccga tagcgtcgat gccgtactgt tttgccagcc agatggcgcg
    13141 ttggttatga aattcttgcg agataatggt gatatggttt tccccaaaca cctttttggc
    13201 acgcactacc gaatccagcg ttgagaatcc ggcatagtcg caaaagatca ctttcgccgg
    13261 tacgccttta gcgatcaaag cctgctgcat acctgatgct tcatcgtaat ttttacgtcc
    13321 gttatcgcca ctcaccagta gccatttcac ttttccggcg tggtacaacg ctgctgcggt
    13381 atcaatacgt cgggtaaagt agcgattacc cggcctcgcc cccagcaata aacccacgtt
    13441 acgcgccggg acggcgttga catcgcccca ggtcagctgc ttgctggcgt tgaccataac
    13501 gcgatcggcg ataaaaatgg tcgctcctgc cacaagtaac agtaagcagg caatctttag
    13561 agtccggcga gaaaaacaaa tacggagaag aaggcgggca aatgcgcgca gcatggtcgt
    13621 tatccttaac gaaagggggg aggtcgattt taggcgctaa agaaaagata acgcctgaca
    13681 tcattttcag attttgtcct gcccaagcga caactgcgtt agccacagcc gggtgtcgaa
    13741 ctcaagctgg tggtactgcg gctccatatg gcagcacaat tgatagaacg ctttgttatg
    13801 ctctttctcc ttcaggtgcg ccagctcgtg caccacgatc atgcgcaaaa acggttccgg
    13861 cgcgttgcga aacacggtag cgacgcggat ctctgctttt gctttcagct tgccgccctg
    13921 cacacgagaa acagcggtat gtaacccgag cgcgtttttt agcacatgga tcttattgtc
    13981 atacatcact ttattgatcg gcggggcatt acgcagaaac tgatttttca gatcctgagt
    14041 atattgccag agagctttat cggtggcgta gtcgtgtgtt cccggatagc gttttgccag
    14101 cacatcgccc agacgctgtt cgttaatcag tgttcgcacc tgcgaaagca gctgctcggg
    14161 ataaccctga agataagtaa gattgctcat aaccgcccac gaaaaagaga aaaagggtat
    14221 actcacgcac ccttttcagg ggcaacgtcg aaattttatc attcaggagg gccgatgagc
    14281 cacttagaca acggtttccg ttcactgaca ctacaacgtt ttccggcgac ggatgacgtt
    14341 aacccgctac aggcgtggga agcggcagat gaatatttgc tgcaacagtt ggacgacaca
    14401 gaaatccccg gcccggtgtt gatcctgaat gatgcctttg gtgcgttaag ctgcgcgctg
    14461 gcggaacata agccgtacag cattggcgac tcatacatca gtgaactggc gacgcgcgag
    14521 aatttacgcc taaacgggat tgatgagtcg agcgtgaagt ttctcgacag caccgccgac
    14581 tacccgcaac agccgggtgt ggtgctgatc aaagtgccga aaacactggc attgctggaa
    14641 cagcaactgc gtgcgctgcg caaagtggtc acgtcggata cacgtattat tgccggtgcc
    14701 aaagcccgtg acattcacac ttccacgctg gaactgttcg aaaaagtgct cggcccgacc
    14761 accaccacgc tggcatggaa aaaagcgcgc ctgattaatt gcacgttcaa tgaaccgccg
    14821 ctggccgatg cgccgcagac cgttagctgg aagctggaag gtactgactg gactatccat
    14881 aaccatgcga atgtcttctc ccgcaccggg cttgatatcg gcgcgcgctt ctttatgcaa
    14941 catctgccag agaatctcga aggggagatt gtcgatctcg gttgcggtaa tggcgttatt
    15001 ggtctgacgc tgcttgataa aaacccgcag gcgaaagtgg tgtttgtcga tgaatcgccg
    15061 atggcggttg cttccagccg tttgaacgtt gaaaccaaca tgccagaggc gttggatcgc
    15121 agcgagttta tgatcaacaa cgcgctctcc ggcgtggagc ctttccgctt taatgctgtg
    15181 ctctgcaacc ctccgtttca ccaacaacat gcgctgaccg ataacgtcgc ctgggagatg
    15241 ttccactacg cccgccgctg cctgaaaatc aacggcgagc tgtatatcgt tgccaaccgc
    15301 catctggatt acttccataa actgaagaaa attttcggca actgcaccac tattgcgacg
    15361 aataataaat ttgtggtgct gaaagcggtg aagctgggac gtcgtcggta agattgggag
    15421 ttacctgccg gatgcggcgt aaacgcctta tccggcctac gttcagcacg ttacccctag
    15481 gccggataag acgcggcaag cgtcgcatcc ggcaatgtct gctcagttct ggaatcgcgc
    15541 ttccataaaa caaaaaatcc ctgtcaccat cctctacatt ctcttgttta gcgtttttct
    15601 acgtttattc ttccgtcaca cagataaatt catccgttgc caatctgtca acggatgtta
    15661 tcatattgcc aatttgtcaa cggacgtgat gaagaatgca cctgataact caaaaagcat
    15721 tgaaagatgc tgcggaaaaa tacccgcaac ataaaacgga gttggtggct ctggggaaca
    15781 cgattgctaa gggatatttc aaaaaacctg agtcattaaa agcagtattc ccatctctgg
    15841 ataacttcaa atatctggat aagcattatg ttttcaatgt tgggggcaat gaattacgtg
    15901 ttgtagcaat ggtctttttt gaatcgcaaa agtgctacat acgtgaagtt atgacgcata
    15961 aagaatacga tttctttacc gctgttcatc gtactaaggg gaaaaaatga ttgcgattgc
    16021 cgacatcttg caagcaggag aaaagctaac tgctgtggca ccttttctgg cgggtattca
    16081 gaacgaggaa caatacaccc aggcgctgga actggtagat catctgctgc tcaacgatcc
    16141 tgaaaacccc ttgctggatc tggtgtgtgc caaaataacc gcgtgggaag aatcagcgcc
    16201 cgaatttgcg gaatttaatg ccatggctca agccatgcct ggcggtatag ccgtgattcg
    16261 tacccttatg gatcaatatg gtttaaccct ttccgatctg ccggaaattg gcagtaaatc
    16321 tatggtgtca cgcgttttga gcgggaagag gaaattaacg ctggaacacg ctaaaaaatt
    16381 ggcaacgcga ttcggcattt ctcccgcctt gtttattgat taacgcgacg tgccggatgc
    16441 gttgcatccg gcgcttcaaa taacgattta aatctccagc gccagccgtg ttccctgggc
    16501 aattgcccgt cgtgcgtcca gctccatagc cacatcgcag ccgccaatta aatgcacagt
    16561 tttcccgcta tcaatcagcg gttgcgccag cgcgcggttt ggctcttgcc ctgcgcagat
    16621 caccacattg tccactgcta atacctgcgt ttcgccgttg atcaccacat gcagcccgtc
    16681 atcgtcaatc ttctgataac ttacgcctgg gatcattttc acaccccgcg agagcaggat
    16741 ggtgcgatgg atccagccgg tggttttgcc taacccctgt cccggtttgc tggctttgcg
    16801 ctggagcatc acaatttgcc gtgggctacg cgggatctgc attccctgcg ggcttaagcc
    16861 accagcctgt tgtaggctgc tgtcgatccc ccactcatta cagaacctgg cgatattctg
    16921 gctggtggat tcgcccggct gacttaaata catcgccgta tcaaaaccaa tcccgccaca
    16981 accgatgatg gcaaccttgt tgcctaccgg cgctttgtcg cgcagtacat cgagataact
    17041 caataccttc ggatgatcga tcccgtcgat aggcggaaca cgcggcacga tcccactggc
    17101 gaggatcgtt tcatcgaacg cctgtaactg atccgccgtc acggtgtgat tgagtttcag
    17161 cgtcacgccc gtcacttcga tcatccggcg gtagtagcgc agcgtttcgt aaaactcctc
    17221 tttgccgggg atctgtttgg cgatattaaa ctgcccgccg atctcgctgt gcgcatcaaa
    17281 caatgttacc tgatgcccac gcgccgccgc gttaatggca aacgccagcc cagcaggtcc
    17341 agcgccgacc accgccagat tttttttctg cacggcggga aggattggca ttttggtttc
    17401 gtggcaggcg cgaggattca ccaggcacga ggtgactttg ccaacgaaga tctgatcgag
    17461 gcagccctgg ttgcagccaa tacaagtgtt gatctcatcg gctcgccctg attgcgcttt
    17521 tgacagcagt tccgcatccg cgagaaacgg tcgcgccatc gatactatat cggcatcgcc
    17581 gcgcgagaga atatcgtcgg caacctgcgg atcgttaatc cggttggtgg ttaccagcgg
    17641 cagcgagacg tggcctttca gtttgcgcgt gacccagcta aatgcgccgc gcggcacggg
    17701 cgtggcgatg gtcggaatcc gcgcttcgtg ccagcctatt ccggtgttga taatggtcgc
    17761 gcccgccgct tcaatggcct gcgccagttc taccgtttcg gcaaaagtgc cgccgccttc
    17821 gaccaggtcg agcatcgaca accggtaaat aataatgaag tcgttgccga cgcgttcgcg
    17881 caccgtacgc actacttcta cggcaaatcg catccggttg cggtaatcgc cgccccactg
    17941 gtcactacgc tgattggtgc gcagcgtcag aaattcgttg atcaaatacc cttcagagcc
    18001 catcacttcg acgccatcgt atccggcctc ccgcgccagt tgcgcgcagc gggcgaaatc
    18061 gtcgatcagt tgcaggatct cttcatgggt gagttcgtga ggaacgaaac ggttgatggg
    18121 ggcctgcaat gcggacggtg cgaccagatg cggttggtag ctgtagcgcc cggtatgcaa
    18181 aatttgcagg gcgattttgc cgccttcctg atgtacggct tcggtaatgg tgcgatggtg
    18241 tgggatctgg ctggcgtcgt tgagcattgc gccaccttcc atgccaacgc ctgttaaatc
    18301 tggtgcaatg ccgccgctga caatcagcgc cacgccgtga cgggcgcgtt cggcataaaa
    18361 cgcagccagc cgttcagcac cgtccgggta ttcctccagc ccggtgtgca ttgaacccat
    18421 caacacgcgg ttttttaacg tggtaaaacc taaatccagc ggggcgaaca gcgacgggta
    18481 gctcataaaa ttgtccagta tgtaaaataa ttgttatgta gtcggatgag ttctaattta
    18541 gccgtcgatg gagaaaaggg aaaaggggag tgcggggttt gtgatgggat tcaaaaagaa
    18601 tacgcccggt ggtaacgccg gatgcgacgc ttgccgtgtt ttatccggct tgggggattg
    18661 cacatgtagg gcggataagg cgttcacgcc gcatccgcca gccgttgcaa attactgctt
    18721 acggaaaaaa tcgttataca acatatacaa atgagcggca ctccaggaga aatttggtgc
    18781 gccttgctgc gcgcctgtca gcgggttgta attctcctga attgggccat cggcggttaa
    18841 tcctttggcg tgcctgaaga acgtatccgc cagcttcagg gcatcatcac gataaccgta
    18901 acgctccatc cctttcagac caaaccagaa ctgatccacc catacgcgcc cgcgccagta
    18961 gatatcagcg ccaaaagccg gattggttaa cgccgccgtt cccagcggaa caaaggtgtt
    19021 gaactcttta ggatcgagca tcaccttcac caccgcgtcg gcattggcct gcgttgccgc
    19081 accgttaaac agcggcgacc agccttccgg ccctttaccg cgctcaacaa tcggtttgcc
    19141 cgcgcagccg ttcgccagtg gtttatcttc aatacgtacg tcatagtaga actgggtagt
    19201 cgggtcgaac atacaggtgt tgatgtagtc cgcgagctgc tgtgccaact ggcgatagcg
    19261 tttggcctct tctggcttac cgaggatggt tgccatctcc gccagataat ggttatcgct
    19321 gtacatatag ctggcctgat ccaccgactc ctgcaatagc gagtagccca gcaacgttcc
    19381 gtcctgactg cggttttcgg cgaatttcac cgtccagtcg ctacgtttgc cgccattagc
    19441 gacatattta tccaactgtt ctttgtcgat aaaaccaaag acggcggcat catcacggcc
    19501 cgattcccac gacgcagcga cctgtgccgg aatttccaga ctgtcgtact ggcctttctc
    19561 caccacgcgg gcgtagttgt tcagcccaga ctgcgtctct tctttatcgc cttttttcac
    19621 cgtaaacagc atctcgccgc tctcggtgtt gtgggctttg tcgcgggtcg cgccatattc
    19681 cggcaccccg ttgccgttat gatcgcggtt acgtaaccac cagtcgtgat aggccaccag
    19741 tttcgggtac atctctgcca accaggcttt atcctgggtg acgttgtata cttccatcac
    19801 cgaccaggcg gcaaggctgg gtttggtatt acgttcgttc cagttgccgc catcgccacc
    19861 acgctcgggg ctaaggttcc acgctatcag gtcggggaca aagcccacat cctgcggacg
    19921 cacgctgtcg ccaggctgga tctgccagga gaagaccgcg cggatattct ctttggcgat
    19981 atccggattg aaatgcgcca tcgcaaacgc ctgcttccag gtatcccacg gccaggtctg
    20041 attgccggag aaccagcgtc cggtcaccga cggcgtgacg gtgttataat tcaccgcacc
    20101 gcccggtgag cgccagttac cgttgagcgt ttcgatcgct ttcaccgcga cgcgcgtctg
    20161 ttccggcgtc gcatccggat tggttaaccc tttcttcagg tattcttccc agcgttgctg
    20221 cgaggcggtg agataaaacg ccggacgcgc cagaatatcg cggatctgca tttgctcttt
    20281 gctaacttcc tgagcggtca gcagatggga ataggtggtg tagagcgtgg tcgaaccgtt
    20341 gatatgcgcc ttactggtaa agcgattacc gttgatttca gtctgcaccg gcagggattt
    20401 atgcacctga tattccgatt cgccggaggt cagcagatcc caggtggcgc gcactttgcc
    20461 aaaggtgact ttcaggccat cacgggtggc gctgattttg cgctgatagt cagggtattc
    20521 gccagcaatg gttttatcgg aaagcggttt cccttctttc gcttccagtt tttccagcag
    20581 ttcgccatcc cacaccagat ccagcggttt attgctgatg attttagttt ccagtagtga
    20641 cgtgcgcggc gtggcgaagc gcagagtcat ttcgacctgc acatcttttg ctgtcagttt
    20701 ttgcaccagc gcgccgggaa tactgtatgc ctccagcgtg aagttgactt tcttgccgtc
    20761 ttgccagaca gtcaggcggt cgaaattgct ggccataaag ttgatgtatt cttccgtcag
    20821 cagcgcaacg cccggaaagc cgcccatggt gttcgggccg tctggcaaca gatgaccatg
    20881 ccaggctccg agatcgaaaa acggattaaa acgctggtga tcgtcgtaat cgtaatcctt
    20941 catgtactgc ggcgcaccag tacggttaat cacgttttta taattgtcgg cgttagcggc
    21001 atgagcggaa aaacttatca gcagagcgca ggttactggc gttaaaatag ttttgatttt
    21061 catgagatta cagtccttat taccagtaga aatattgcat caggaagatt tggtcgctgg
    21121 gatcgttaga ggcatttttc atgtagaagc ggctatcaaa agccgtggcg aaacgtccgc
    21181 cgccatattc gtaccagata ccaaactgca tgaaagaggt ggtttcatcg tcagcgtggt
    21241 cagggcgatg tttggcgtag gtgtaagcgg tggagagata aacgcctttt aacgcttcat
    21301 acatggcgct caccatcatc ccgctttcac ttcccggtac gtccagcccg tcggcacgtc
    21361 cggtatggtg ccaggcgcga gcggcaaagt taggggtaaa caggcccgtg agatagagtt
    21421 gtcctgtgcc gtcggtaatt tccagaccat tcatccaggt gatgccttcg gcaaggtcat
    21481 actggatgta accgttcatc cattgccgga taggtgtatt tgtcgtcgta gcgatcgtac
    21541 ttgccaaaat gtagccaggc tttgctttcg tcatgatgtc cggcaggtgt ggcggtgacg
    21601 ctataacgaa agttgccgct taagttctgt accttcagcg cggtcataat gtcgcgggta
    21661 ttgggaatga cgtagcccaa atcgggtgtg aagtcgcccc accattgcag gtcatccagc
    21721 gaggaatcct gacgcagact gagcatgatt tcggtgccat cattggtacg ataaccgcca
    21781 tagaaacggt taatcccacc ttcgaaacca ccccagctat ggtcaggcac ctaggcatta
    21841 ccgtcgtggt cggcctgcac ggtccagcct tcgccgtaaa tcaggccgaa ccaggagccg
    21901 tacttcatct ccaggccacc ttcaatatag gtgccgtcgc tgtatttatg tttattctca
    21961 ccctctaaat ccatatatcc accaagccct aattcgccgt aaaaacgaaa tgaaggagaa
    22021 gatgtcgagg taattgcggg tgtttttgtg tcgtcatgtt ctgccggacg tgcttcttct
    22081 cctgccgcag aaaaggcgta gcaaaacggc agagcaagta atgctctgca tggtgcagtg
    22141 attaatttca ttgtgttaat ccatgagcta aagaaaaagt acagcctttt atcgttgtaa
    22201 ttataaaaat ataaaggcag aaactatttt ccctctcctc atccaggaag agggaaaatg
    22261 cctgctcatt ttttattagt ctgcttaatc aacattgctg gctggcgtcg cttcaatatg
    22321 gcgctcttca gccgttaatc cctttatata tttgctgtat ttccaccacg caaagccgag
    22381 gaagataaca ataccgccga cgttataaaa aatgatggtc agaatattcg cgccagtcgg
    22441 gaatgtcgaa gcgacaaacc ctacggcaaa tatcgcaatc agcattgaaa caacgataat
    22501 accggtgcgg cgggagccca tacggaaatc gcgtggcagg tgatctaatt tggcgcgtaa
    22561 attcaggtaa gccagcatga tgaataacgg cggaagcatg gacgctgcgg cggtcatatt
    22621 aataatggta ttcatcagat cctgcacggt attggaaccg agcatcggga taatcatcag
    22681 cgggatgacg atcagaaact ggatccatgc tgcgcgcgcc ggaacgccgt tttcgttaag
    22741 ttcgacggtt ttcttaccaa agatgccttc cgggatttcg gagaagaaaa ttttcaccgg
    22801 cgttgcggtc cacatcagca gggaaccgaa cattgcggta aaggacacca gaccgacaaa
    22861 gcgattcatc aacgcttccg gcagaccaaa atacgccgcc atgccgtgga atacctgcac
    22921 cgatccgccg gtaaatttca actctttgct gctgacgaag acgttaatca gcaccgagga
    22981 gacggaatac agtacgccga taaaaatccc ggcgaggatg atcactttaa cgaacgattt
    23041 cgaaccgcct ttgacgtcgt taacgtacac cgcgacggac tccgcgccac ctgcggccat
    23101 aaagatccag gtggtaacgc cgaggaacgc ccagttgaag ttcgggatca tcgcgtcaac
    23161 ggtgatcgga tcggcaggct gtacgccgcc aaccagtgcc gtacctgcca gtaaaatgta
    23221 ggagagcgtt aacagcagca tcagcgttga agtgacggag gtaattggcc ccagcatttt
    23281 cgccccgttg gtggaaaccc aggtggagaa ggcgaacagc accatactga taacggtggt
    23341 ggcaaccggc gtcataatat attcgtagcc gaggaaggca tacgaagcat aagcaataac
    23401 gcgcggcaac agcgaggtga aaaagaacag atttacgaac cagtaagtgt aggcagtaat
    23461 aaatgcccaa cgtccgccca gcgaactttt tacccacgcg tagacaccgg cttctgagtt
    23521 tttatttaac gaaacaaatt ctgcgatgat cagacagaag ggaataaaat aaaaaatcgt
    23581 cgcgaggaaa aacatcggtg ccgaggccag tccaagctca atattattat tgataacgtt
    23641 gttaaagctg taaacggcgg caaaagtcag cgagagcaag ccgaatttgc cgattgtatt
    23701 acgtttggta tcagacataa cacatctccg aaaatgtgta gggtagcggt ggcggcaaac
    23761 tccttttgcc gccgtagttg ttatttgtta tggaaataac catcttcgat ggtgactttg
    23821 agaaccactt ttttgaccgc gttattgcag ataaaccgat acgcttcatg gatatcgcaa
    23881 ataacgattt gcccttcgtg gacctctacg gtttctccgc agccttttaa atattcacgg
    23941 tcagtttcat cacgataata ttccactacc tgtaatgtct ctttcggcgc atattcaatt
    24001 ttttgctgcc cttgcaggta gtaatgcact tcaaaatagc gtcgatgccc ggtaaatagc
    24061 gcatcggtcg cggagtcatt ttccacacgg taggtcaatg aatcaccgat ggagtgggcg
    24121 acgccaggct gaatgttgtc gatattttca atcgcttcaa cgcagcgttg ccacttcttg
    24181 ccagaggcgt aaatctggcg gaactgttct aagttatcga tgatcctcat tgcttattct
    24241 cgctgtgcaa attcgtggaa aagaaccctg cgccgaactc atacgacgcc aggctttgcg
    24301 cggtagcttc tccgccagaa accggcagca acgtaaagcc gtagctgaag tcacggaacc
    24361 agacgcgcca ggagtccagc acctcgctgc cccaggagtt ggagccgagg ccaagtagct
    24421 ggtggtcgag gttcagggtg atgtcatcac tgcgctgcag ctcgttacag tgctgggcag
    24481 cgtggatgtt ttcctgggta tagtgccagg cgctgaagtt aattgggcgc tgcggaacca
    24541 ccagcagacc gttgccgtgg cggttagtca gtgccgtcca gcggacatgc tgacggttgc
    24601 cgttgttctg cgggaaggga tagttctcga acatggcatc gacggtgctg cgccagatat
    24661 cgatgatgtt agcctgctgg ctgtcggcgt agttttcgcc cggtccacga ccgtaatacg
    24721 ccacctgatc gtattcgccg ttaatcccca tggtgaaacc gatgcacgga atgatgtgcg
    24781 gatagtcgcc gtaacgctcg ccggaaagcg ccacgttaac ctggccatcg gcagcgatgc
    24841 gccagatgta ggtacagcgc atcccgaagt caaacaccgg cggtgcaata actgtgcggc
    24901 tgatgatcaa cacttcacca tcgctctgtt ctacggcaaa gtcgcgcaga tgttcctgca
    24961 tgatctgcaa atgattcggc tgccacaacc cttcgtactc ctgcttgtgg ttgtcgatca
    25021 tcggcttgaa gaagttgatc tttggctcgc gggtcagcag cgactcgcca tttacctggc
    25081 aggatgtcgg tttgccactc atttttgaga aggtgatcgc gaagttgtag ccgcgaacgg
    25141 tgcagctcaa acgatcgtct tccagcgtca gcggacgcgc attatttggt gcgaaaggca
    25201 ctggctgcgc ggtgttttcc ttcagcggga actgataaga ggcgatagaa tgcccggctt
    25261 cgctgtagcg ggtgcgggaa tctttggtca ccgtaatgtt gaggaacgct tcgcgggcgt
    25321 ccagctgcgg cagcgtgatc tgcaaggggg cttcgctgtt cggcgcaacg tcgcgcagtt
    25381 taatctgctg cgtcgcgagc gtttcacctt cggcgcgcac ctctgcgtgc agggtgtagt
    25441 catcaagcgt ggtaaaccac agtttatttt cgactttcaa ctcgccgcga gtcagcgcgt
    25501 ggatttttac cggcgcgata acctgtttgt actctttcag gcccgatccc ggcgtctgat
    25561 cggaatagat caaaccatca agacagaagt tatagttgtt gggatagtcg ccgccgaatt
    25621 tataccagac attgccgtgg tcgtcctgtg cctggatccc gtggtcgcac cactcccaga
    25681 cataatgacc ctgaatgcaa tcgtgcttat agaagacgtt ctggtactcc gtcagcccgc
    25741 ccggtccgtt ccccatcgca tgagcatatt cacagatgat gcgcggcttc ggatgcgggt
    25801 attcaccaaa ctcattcatc agcggcacgc gggtgtacat ggtggaaata atatcgacca
    25861 cttcagcatc gcgatcttct tcgtaatgaa ccagtcgcgt gtcatccagc gctttcgccg
    25921 catggtacat cgcgcggatg ttacagccat agccggattc attgcccagc gaccagatga
    25981 tgatcgacgg atggtttttc tgtgcgtgga tatggcgaac aatgcgctcg acgtagactt
    26041 tttcccactg cggatcgtcg gtaatacggc tgatatcgcc gacattagca aagccgtgcg
    26101 attcgacgtc ggtttccgcc atcacaaaca ggccgtagat atcacacagt tcgtaaaaac
    26161 gcggatcgtt cgggtagtga gcggtacgca cggagttgat attgtgctgc ttcatcaact
    26221 ggagatcttt ctcgacgcga tccattccaa cggcgcggcc tttgcgatga tcgttgtcgt
    26281 gacggttgac gccgtgcagc atcacataac ggttattgat ccagaacaga ccgtcgcgca
    26341 ctttgatatc acggaagcca acgcgttgtg gcaccacttc cagaacgttg ccgttggcgt
    26401 ctttcagcgt catgaccaga tggtaaagat aaggggattc tgctgaccat tgctgcggct
    26461 gttcgacagt aaaagcaaag ctggcgctgg tcagtttttc aattgccaaa tgatcaatgg
    26521 cgctgctgtg caccacgcgt tcgccatcaa acagggtata ttccagcgtc gtgacgacag
    26581 gggaggcggc gagattttcc agcaccactt cgcaggaaag cgtggcatcg caataggctt
    26641 cgtcaaagtc ggtacgcaca gtgaaatcgt taatatgcgt taggtgtttt ccgaccagat
    26701 aaacatcgcg gaagatcccc gccgaccacc acatatcctg gtcttccacg taggtagagt
    26761 ccgcccactg catcacgcgc acacacaaca ggttgtcgcc ggttttaacc atcgcgctga
    26821 tgtcaaactc tgcggtcagg cgactgccct tgctgaaacc cacatactga ccgttaacgt
    26881 agacttcaaa ataggtttcg acgccgtcaa atttaatcag cgtctgttta ccctgccagc
    26941 cgtcgctgag ggtgaaaata cgttgatagg caccggttgg gttatcgctg gggacaaacg
    27001 gcacatcgat ggggaacgga aaaccttcgt cggtatattg cagtttgccg tgaccttcca
    27061 tttgccacat ggcggggacg gtaatatgcc cccagtcagc cattaactca gaggtgaagg
    27121 cttctggtac ttgcagcgga tggtcaaaaa agtggaaatt ccactgactg cttaagggca
    27181 gaaacaggct gctggtttcg cgggcaaagg tacgcgcttg cgcaacagaa tcatatgaaa
    27241 aaaagtacgc acgcggcgca agtcggtttt cgtgggtgag ctgaatgttt tcccagcgat
    27301 tcatagggcc tcccggtaga gagaaatcgg aaccattcat ggtgatggta gtttgtgtaa
    27361 ttgctggggg gttagtaaaa tttttactaa aaagcagcag gtaaatgcat ttactttaac
    27421 tggatcacga aaataaacgg cagcactgtc cggcaaaaat gattgccgga cagtgtgtaa
    27481 ggggatttaa cgggtcgtgc cgcgcagttt taatttgctg ggaacgaaga ctagcagcgg
    27541 cagcgcgcga ccatcgcggg ctttttcata caccaggtta acgccctgac ttcccatcat
    27601 ctcggaatgg atgcgcacgg tggagagcgg cggaaaggta aatcgcgcgg tggggatatc
    27661 gttaacgctg ataagcgaaa tatcttgtgg gatgttcagg ccacgttcat gaattgcccg
    27721 cagtacgccg atagcaatgg aatcggaagc aacaaacagt gccttcggat agtcttcccg
    27781 cgccagcatt tgttttgcca gttcataacc cgacgaactg gaaaaaccgc cgcgccagat
    27841 atcctcttcg cgtaccactt gtttcagtcg gccatattcc gcaaaggcga cctcacgaat
    27901 atccgccttg ccaggctcat cttcaccgcc aataaaacca atacgattaa cgccctggtt
    27961 gatatagaag tcgatgattt ctttactgat gcgtgccaga tcgatatcca ccgcatcgta
    28021 accgctgccg ggttcgtgaa agtcgataaa acagatattg tcggtcaacg cgctggcagc
    28081 ggcgcgcagg gcgggcgtgg gtttgccgac aattaaaata ccggtgacgt ttttaatgtc
    28141 tggtaatccg ttgtgttcat aacagttggt cagttcgatc gccagctttt cgcactgagt
    28201 ttcaatgccg tggcggatcg ccagatagta aggatcgttg atctccagct cctgctggta
    28261 gctgtagata gccagaatat ggtgttggtt gactgcgcct gtctgtagtt tacgggcgct
    28321 actggtcttg tactccagct tttcggcgat ctcgagaatg cgatgtttcg tctcttcttt
    28381 cacattcaat gtcggatcgt catttaagac cctggatact gtcgccaggg atacgccagc
    28441 ttcgattgcg atgtctttta atgttgccat gtttaccttt tttactttcc ctgatgtgcg
    28501 cccccattgt agaggaagtg cttatcgggt cacctgtttt cagtaaataa agcgcgtttc
    28561 tttgacgctg taacggtaac acagcggctt aggaatgtga tcacgcctgc attctccatt
    28621 tttagcctac aggattgaga aaaacttttc tatagtttag taaaatttta tccgaaggga
    28681 ggaagcaatg gaaaccgtgg cttacgctga ttttgcacgt ctggaaatgc gcgtcggaaa
    28741 gattgtggaa gtgaaacgcc atgaaaacgc cgacaagctg tacatcgtac aggttgatgt
    28801 ggggaaaaaa cactgcaaac cgtgaccagc ctggtgccgt actacagcga agaggagctg
    28861 atggggaaaa cggtggtggt cttatgcaat ctgcaaaaag cgaaaatgcg tggtgaaacg
    28921 tcggaatgca tgctgttgtg tgcggaaacc gatgacggca gtgaaagcgt gttgttaacg
    28981 ccggagcgga tgatgcctgc gggcgtacgc gtcgtataaa aagatcggat ggcgacgtcg
    29041 tatcgccatc cgatttgata ttacgcttct tcgacactta ctcgcatggc cgccagcgcc
    29101 ttacgcgccg ctttgatcac cagttcacac tgttcaatgg tcagtgtcag tggcggttca
    29161 atgcggatcg ttttggcgtt attgagcgtt ccggccacca gtacgcgctg gcggaacatc
    29221 tcgctggcaa agttatagcc gatttcgtta tcaacaaact caatcgccat caacatccct
    29281 ttaccacgcg cttcctgtac cagatcggga tattcccgcg ccagttgacg gaaaccgtcc
    29341 agcaacatat cgcctttttg ctcagcctga gccggtaagt tctgctccag caacacattg
    29401 atggtcgcca gcgccgccgc acaggccagc gggttgccgc caaaggtggt ggtatgcagg
    29461 aatgggttgt cgaacagaac tgaaaacacc tcttcagtgg cgatggtcgc gccaatcggc
    29521 atcacgccgc cgccgagggc ttttgccaga cacagaatgt ccggctgaac gttctcatgt
    29581 tcgcaggcaa acatcttgcc cgtgcgcccc atgcccgttt gtacttcatc gaggatcatc
    29641 agtgcgccga actcatcgca cagcttacgc acggcggtga gatagcccgg cggcggcaga
    29701 attacgccac cttcgccctg aatcggttcg aggatcaccg cagccacatc atcaccggtt
    29761 tttttgcact cattaagagc cgtgcgcatg gcttcgatat tgccaaacgg cacatgacgg
    29821 aaccctggca gcagtggcat aaacggtttg cggaaggtcg atttcgccgt ggccgacagc
    29881 gcgccaagtg atttaccgtg gaacgcgccg ctggtggcaa taaaagtaaa tttgccgcgc
    29941 ggcgactgat aagccttcgc cagcttcagc gccgcttcaa cggactcggt gccgctatta
    30001 cagaagaagc tgtatttcag tttaccgggc gttagcgcag caagggtttt cgccagcata
    30061 gcccgtaacg gatcgagtag ctcctggctg tgcagtggtt gtttcgcaag ttgattctgt
    30121 acggcggaaa ccacaactgg attacggtgc cccacgttga aaattccaaa acctcccagg
    30181 cagtcgataa actcctgtcc ctgggtgtcg acaagcgtat ttaaacctcc cgcttgccac
    30241 tctacggctc cgtaatcccc gccggcggta acagatttgc gatactctaa aaaccccgga
    30301 ttgacatgct ctttgaagta ttcaatcacc tctcggttaa gtgctttcat ctcctcatga
    30361 tccagcgttc gcttctcaat gagattcagg gcgtgggcgc tgcacgctaa agccgatgcg
    30421 ctcgaaggta acctgttcaa aatatgctcc ggaggctcgc gtatcacatg ataccgattt
    30481 aagtattgca gggattgcgc cactccggct gcgatcgcga aaatcgggat aaacacaaat
    30541 gcggttatca tttgtgcaat atttaatgct atccgttaac atttgttgtt attttgcacg
    30601 gtttttgtcg ggtatggatt tgatgattgc tgcgcaaatg cacgttttca gggcaataat
    30661 tgcctgattt ttgggcaaga taattaacgt tttcgtatag taacagagtg aaagtatttt
    30721 aaatcaaaga attagatttg tgctttaatc gcaaattgcg atctaaatca aattaatcgg
    30781 ttaaagataa ccgcagcggg gccgacataa actctgacaa gaagttaaca accatataac
    30841 ctgcacagga cgcgaacatg tcttctcatc cgtatgtcac ccagcaaaat accccgctgg
    30901 cggacgatac cactctgatg tccactaccg atctgcaaag ctatatcact catgctaatg
    30961 acacttttgt gcaggtgagc ggctttacct tgcaagagtt acaagggcag ccgcacaaca
    31021 tggtgcgtca cccggatatg ccaaaagcgg cgtttgcgga tatgtggttc accctgaaaa
    31081 aaggggagcc ctggagcggc atcgtgaaaa atcgccgcaa aaatggcgac cattattggg
    31141 tgcgggccaa tgcggtaccg atggtgcgcg agggaaaaat cagtggctat atgtcgattc
    31201 gtacccgggc gacggatgaa gagattgcgg cggtggagcc gctgtacaaa gcgttgaact
    31261 ccggacgtac cagtaagcgt attcataaag gcctggtggt gcgtaaaggc tggctgggta
    31321 aactgccttc attaccgctt cgctggcgga cgcgtggagt gatgaccctg atgtttatct
    31381 tgctggcggc catgctttgg tttgttgctg ccccggtggt gacgtatttc ctctgtgtgt
    31441 tagtggtatt gttggcaagc gcttgttttg aatggcagat tgtgcgcccg atagaaaatg
    31501 tcgcccgtca ggcactgaag gtggcgaccg gagagcgtaa tagtgttgag cacctgaatc
    31561 gcagcgatga gctggggctg acattacgcg cggtagggca gcttggcctg atgtgccgtt
    31621 ggctaattaa cgatgtctca agccaggtgt ccagtgtcag aaatggcagt gagacgctgg
    31681 cgaaaggcac cgatgaactg aacgaacata cccagcagac agttgataac gttcagcaaa
    31741 cggtggcgac catgaaccaa atggcggcgt cggtgaaaca gaactctgcc acggcgtcgg
    31801 ctgccgataa actttctatc accgccagta atgcggcagt gcagggtggg gaggcgatga
    31861 ccacggtgat caagacaatg gacgatatcg ccgacagtac ctagcgcatt ggcaccatta
    31921 cttcgctgat taacgatatt gcgtttcaga ccaatattct ggccctgaat gcggcggtgg
    31981 aagcggcgcg tgccggcgaa cagggcaaag gttttgcagt ggtggcaggg gaagtgcgtc
    32041 atttagccag ccgcagcgct aatgctgcca acgatattcg caagctgatt gatgccagtg
    32101 ctgataaggt gcaatccggt tcgcagcagg tacacgccgc cggacggacg atggaagata
    32161 ttgtggcaca ggtgaaaaac gtcacccagt tgatcgccca gattagccat tcaacgctgg
    32221 aacaggccga tgggctttcc agcctgaccc gtgcagtgga tgagcttaac ctgatcaccc
    32281 agaaaaatgc cgagctggtg gaagagagtg cgcaggtgtc ggcgatggtg aaacaccgcg
    32341 ccagccgact ggaagacgcg gtgacggtgc tgcattaatc ggtatgccgg atccggcggt
    32401 tggagcacaa tgcctgatgc gatgctggcg catcttatca ggcctgcggg gtgtgtagcg
    32461 ggtgtaggcc ggataaggcg cgtcaagcgt cgcatccggc aattgcaccg cgccactggc
    32521 ggatgcggcg attaatccag ttgcgtaata tcaaaagcgg cgcggtcgat caccgcaatg
    32581 atctttttta tttgtgcatc actgatatcg ctctggttga cgcgtaaatc cagcaccgct
    32641 ttaaaattat ccagcgcccg cttcatttgc gggttctggc gcagcgccgc gccaacgcaa
    32701 cgcgctttga tgcgttcttc aatcatctcc acttgttcgc ggttttcttc cagccactgc
    32761 gcaccttgtt cggtcagcgc aatctgcttc ttaccacctt cctcttcgcg gatggtaatc
    32821 agcgactgct cctgcagaaa atccagcgtc gggtagatga cgcccgggct tggggtgtaa
    32881 ttcccctggg ttagattctc aatcgcttta atcaattcgt aaccgtggct gtcatcgcgc
    32941 gagagaatat ccagaatcac cagacgcaat tcaccgtgac caaagaaacg ttgccgacga
    33001 ccgccgccgc gaccgtggcg accaccgcaa cattgaccgt gttcatgctg gtgaccgtgt
    33061 ccgcagtgct cgtgttctga cttctcacct ttgcagcagc cctcatgccg tggctggcct
    33121 tcatgtttac aacacccttc gtgatgatgg ctcatatcag cctccttttg cttgattaga
    33181 tatatctaat ttatatctga tgtgagcatt actgcaagta caaaaatgat ttttaaacgt
    33241 ggaagcacct cacatctagg ccggataaga cgttcacgcc gcatccggca gcattgctat
    33301 ggcactgaat gccttcacat acttcagaca tatcagttct acaaatcgct tgcatttatc
    33361 atgattaaca atcattatca tttgcgaatt ttatttagat atatctgatt accatcacga
    33421 aggcgataac aatgaataac accccccgct acccgcagcg cgttcgcaat gatctgcgct
    33481 tccgtgaact gactgtgcta cgcgttgagc gcatcagcgc cggttttcag cgcattgtcc
    33541 ttggcggcga ggcgttggac ggttttacat cgcgcggctt tgacgatcac agcaaactct
    33601 tctttcctca acctgacgct cactttgtgc cgccaacggt aacggaagag agcatcgtct
    33661 ggccggaagg accacgccca ccgtcgcgtg actatacgcc actgtatgac gaactacgcc
    33721 atgaactggc gatcgatttc tttattcacg acggtggggt cgccagcggc tgggcgataa
    33781 acttacggtg gcaggtccgc gcggttcgct ggtggtgccg gaagattacg cgtatcagct
    33841 gtatgtctgc gatgaatccg gaatgcccgc actgcgccgc cgcctggaaa cgttgagcaa
    33901 acttgccgtt aaaccgcaag ttagtgcgct ggttagcgtg cgggataacg cctgtcagga
    33961 ttatctcgcg caccttgatg gttttaatat cgaatggctg gcacatgatg agcaggcggt
    34021 agatgcgcgt ctggcgcaga tgcaaatccc tgccgatgat tacttcatct ggataaccgg
    34081 cgaaggtaaa gtcgttaaga atttaagccg ccgctttgaa gcggaacagt atgacccaca
    34141 gcgggtacgt gcagcggctt actggcacgc aaagtaactg atatataaaa agaaaaaggc
    34201 tgacgatttc tcgtcagcct ttgctatatc tggtggcccc tgctggactt gaaccagcga
    34261 ccaagcgatt atgagtcgcc tgctctaacc actgagctaa ggggccatgg tagcggatta
    34321 taaagtaact ccgtgtcgca atccagccat taccgcgcgc ctgctgtttt tataaacaat
    34381 gtattttcaa tccgttatac ttttcctggt gatgttagaa aggagtaaat atggttgagg
    34441 atattttggc tccagggtta cgggtcgtgt tttgcggtat caaccctggg ctttcatccg
    34501 ccgggactgg ttttcccttt gctcatccgg caaatcgctt ctggaaggtg atatatcagg
    34561 ccgggtttac cgaccgtcag ttgaagccgc aggaggcaca gcatctgctg gattatcgtt
    34621 gtggcatcac caaactggta gaccgtccaa cggtgcaagc caatgaagtt tcaaagcagg
    34681 agctgcacgc aggtgggcgt aagctgattg aaaaaattga agattatcag ccgcaggcgt
    34741 tggcgattct gggcaaacaa gcatatgaac agggattcag ctagcgcggt gcacagtggg
    34801 ggaaacaaac gctcaccatt ggttcgacgc agatttgggt gctgccaaat cccagcggtt
    34861 taagtcgcgt ttcactggag aaactggttg aagcgtatcg cgagctggac caggcgctgg
    34921 tagtgcgtgg gcgataa
//
LOCUS       DAEEGA010000037        34444 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEEGA010000037 DAEEGA010000000
VERSION     DAEEGA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34444
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.037"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..331)
                     /locus_tag="KLH46_001942"
     CDS             complement(<1..331)
                     /locus_tag="KLH46_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378635.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVTADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="KLH46_001943"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="KLH46_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBE0378636.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="KLH46_001944"
     CDS             complement(1692..2072)
                     /locus_tag="KLH46_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HBE0378637.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..2502)
                     /locus_tag="KLH46_001945"
     CDS             complement(2086..2502)
                     /locus_tag="KLH46_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378638.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="KLH46_001946"
     CDS             complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="KLH46_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBE0378639.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3503..3970)
                     /locus_tag="KLH46_001947"
     CDS             complement(3503..3970)
                     /locus_tag="KLH46_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBE0378640.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4135..5004
                     /gene="pldA"
                     /locus_tag="KLH46_001948"
     CDS             4135..5004
                     /gene="pldA"
                     /locus_tag="KLH46_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBE0378641.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5137..6966
                     /gene="recQ"
                     /locus_tag="KLH46_001949"
     CDS             5137..6966
                     /gene="recQ"
                     /locus_tag="KLH46_001949"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBE0378642.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDGEE"
     gene            7030..7650
                     /gene="rhtC"
                     /locus_tag="KLH46_001950"
     CDS             7030..7650
                     /gene="rhtC"
                     /locus_tag="KLH46_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBE0378643.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="KLH46_001951"
     CDS             complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="KLH46_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBE0378644.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8443..9465
                     /gene="pldB"
                     /locus_tag="KLH46_001952"
     CDS             8443..9465
                     /gene="pldB"
                     /locus_tag="KLH46_001952"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBE0378645.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9473..10273
                     /gene="yigL"
                     /locus_tag="KLH46_001953"
     CDS             9473..10273
                     /gene="yigL"
                     /locus_tag="KLH46_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBE0378646.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10349..11248
                     /gene="bioP"
                     /locus_tag="KLH46_001954"
     CDS             10349..11248
                     /gene="bioP"
                     /locus_tag="KLH46_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBE0378647.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11136..12089)
                     /gene="metR"
                     /locus_tag="KLH46_001955"
     CDS             complement(11136..12089)
                     /gene="metR"
                     /locus_tag="KLH46_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBE0378648.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12325..14586
                     /gene="metE"
                     /locus_tag="KLH46_001956"
     CDS             12325..14586
                     /gene="metE"
                     /locus_tag="KLH46_001956"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBE0378649.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14625..15440)
                     /locus_tag="KLH46_001957"
     CDS             complement(14625..15440)
                     /locus_tag="KLH46_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBE0378650.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15702..16463
                     /gene="udp"
                     /locus_tag="KLH46_001958"
     CDS             15702..16463
                     /gene="udp"
                     /locus_tag="KLH46_001958"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBE0378651.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16604..18031
                     /gene="rmuC"
                     /locus_tag="KLH46_001959"
     CDS             16604..18031
                     /gene="rmuC"
                     /locus_tag="KLH46_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBE0378652.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18126..18881
                     /gene="ubiE"
                     /locus_tag="KLH46_001960"
     CDS             18126..18881
                     /gene="ubiE"
                     /locus_tag="KLH46_001960"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBE0378653.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18895..19500
                     /gene="ubiJ"
                     /locus_tag="KLH46_001961"
     CDS             18895..19500
                     /gene="ubiJ"
                     /locus_tag="KLH46_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBE0378654.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19497..21137
                     /gene="ubiB"
                     /locus_tag="KLH46_001962"
     CDS             19497..21137
                     /gene="ubiB"
                     /locus_tag="KLH46_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBE0378655.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21216..21485
                     /gene="tatA"
                     /locus_tag="KLH46_001963"
     CDS             21216..21485
                     /gene="tatA"
                     /locus_tag="KLH46_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBE0378656.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21489..22004
                     /gene="tatB"
                     /locus_tag="KLH46_001964"
     CDS             21489..22004
                     /gene="tatB"
                     /locus_tag="KLH46_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBE0378657.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22007..22783
                     /gene="tatC"
                     /locus_tag="KLH46_001965"
     CDS             22007..22783
                     /gene="tatC"
                     /locus_tag="KLH46_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBE0378658.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22825..23607
                     /gene="tatD"
                     /locus_tag="KLH46_001966"
     CDS             22825..23607
                     /gene="tatD"
                     /locus_tag="KLH46_001966"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBE0378659.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23604..24092)
                     /gene="rfaH"
                     /locus_tag="KLH46_001967"
     CDS             complement(23604..24092)
                     /gene="rfaH"
                     /locus_tag="KLH46_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBE0378660.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24259..25752
                     /gene="ubiD"
                     /locus_tag="KLH46_001968"
     CDS             24259..25752
                     /gene="ubiD"
                     /locus_tag="KLH46_001968"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBE0378661.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGEHFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25798..26499
                     /gene="fre"
                     /locus_tag="KLH46_001969"
     CDS             25798..26499
                     /gene="fre"
                     /locus_tag="KLH46_001969"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBE0378662.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="KLH46_001970"
     CDS             complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="KLH46_001970"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBE0378663.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="KLH46_001971"
     CDS             complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="KLH46_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBE0378664.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30243..31574
                     /gene="pepQ"
                     /locus_tag="KLH46_001972"
     CDS             30243..31574
                     /gene="pepQ"
                     /locus_tag="KLH46_001972"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBE0378665.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31574..32188
                     /locus_tag="KLH46_001973"
     CDS             31574..32188
                     /locus_tag="KLH46_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBE0378666.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32227..33678
                     /gene="trkH"
                     /locus_tag="KLH46_001974"
     CDS             32227..33678
                     /gene="trkH"
                     /locus_tag="KLH46_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBE0378667.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33690..34235
                     /gene="hemG"
                     /locus_tag="KLH46_001975"
     CDS             33690..34235
                     /gene="hemG"
                     /locus_tag="KLH46_001975"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBE0378668.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8103 a         8701 c         9306 g         8334 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggtaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gtgatgatag agatgatata
     2221 tactgctaat gtaccaaaaa cataaatttt tatatatatg aaaccactat cacggagtcg
     2281 ttggcaattc atgttgatga cgagataatg gagtgcgatg gtagagacta taacaagaaa
     2341 acctgcttcc ccatcgttaa aaaagataat aagaaaggca aaaatgaaat ttattaaaat
     2401 aaataaaaat atatatcggt gtctggaaat cttaccgtta gatgttggga taaacatacg
     2461 taacataaat tttacatcct tgtgtgagtc tcctgtcagc atggcaatat gcccactctc
     2521 atacaagagt cggcatattt tttcagaata tatttatttt ttatttggac gttctacgct
     2581 gcgtataaat cgcgtccatc acaaaaattg ccagcgccac ccaaataaag gtgaaagtca
     2641 ccatcttatc ggcacccggt ttttcaccat aaaacgtcac agccagcagg aacatcagcg
     2701 tcgggccaat gtactgaaaa aagcctaacg ttgagagacg caggcgcgtg gcagcggcag
     2761 taaaacacaa cagcggtacg gtagtgacaa taccggcggc gatcagcagt aaattcagcg
     2821 acatcgggtt ttgccccata tggctggttg aactgtcggc aatagcaaac aggtaaatcg
     2881 ccgccacggg cagcagccac atggtttcaa ttaacatgcc ggtttgtgct tctaccgcaa
     2941 tcttcttgcg caccaggccg tagaaggcaa aactaaatgc cagccccagc gcgataatag
     3001 gtagcgaacc aaaagtccac agctggacca acacgccaca tatcgccaga atcaccgcca
     3061 gccattgcat ccggcggaat cgctcgccga ggaaaatcat ccccagcaca atgttcacca
     3121 gcgggttaat aaagtaacca aggctcgctt ccagcatatg gtgattgttc accgcccaga
     3181 taaacagtag ccagttgcca ccaatcagta cggcagagac tgccagcata aaaattttct
     3241 gtggcgtctg aatcagcgtt tttaaatagg accactggcg gcaaatgctc atcagcacca
     3301 ccataaagaa aaacgaccag atcacgcgat gcgtcaggat ctcatcggcg ggcacgtagt
     3361 aaatcagctt gaagtatgct ggcgcaatac cccaaataaa ataagcggca agagcgagta
     3421 atacgccctg ccgcgtttgt tttgcatcca tcgggaatac tcatttttaa ttggtaacag
     3481 cagtttacct gcttttatgt cttcaaccca ccatataggt ggcggtggca ctggcaatat
     3541 aaagctgttc ttcattgtgt aattcaacgc gggcgacggc gactttattg cctgcacgca
     3601 acaggctact ggtagcagta aaacgctcgc ccctgcctgg gcgcagataa tcaacgcgaa
     3661 gatcaatggt ccccatccgc gatagccgct ggcgtagttc atcttcactg atggtttcgt
     3721 ggcgggttaa ggtacttccc acgcacacca ggccggcggc gacatccagc gccgacgcaa
     3781 tgactccgcc gtgcaaaatg ctttgcgccc agttacccac catcatcggc tgatttttaa
     3841 aggccagctg tgcgaactct ttttcgtaac gctccagttc catccccaat gcgcggttaa
     3901 atggcatgtg ataaacaaac atctcaccca ctaatttcag ggcttgttca gcggtcagta
     3961 cggcagacat atcatcctta cacttcattg gttaatgaaa tgttgatttt atgcttcttt
     4021 gttgttggtt tctactttag gaagggataa ctaacggcaa tggagttaag tatgtaaaat
     4081 agcccgcaga aaaacattca ccttatcaat aattcgttac ggagaacacg accgatgcgg
     4141 actctgcagg gctggttgtt gccggtgttt atgttgccta tggcagtata tgcacaagag
     4201 gcaacggtga aagaggtgca tgacgcgcca gcggtgcgtg gcagtattat cgccaatatg
     4261 ctgcaggagc atgacaatcc gttcacgctc tatccttatg acaccaacta cctcatctac
     4321 acccaaacca gcgatctgaa taaagaagcg attgccagtt acgactgggc ggaaaatgcg
     4381 cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc cgctgtggcg tgggatttta
     4441 ggcccaaact cggtgttggg tgcgtcttat acgcaaaaat cctggtggca actgtccaat
     4501 agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac cgcaattgtt cctcggtttt
     4561 gccaccgatt accgttttgc aggttggacg ctgcgcgatg tggagatggg gtataaccac
     4621 gactctaacg ggcgttcaga cccgacctcc cgcagctgga accgccttta tactcgcctg
     4681 atggcagaaa acggtaactg gctggtagaa gtgaagccgt ggtatgtggt gggtaatact
     4741 gacgataacc cggatatcac caaatatatg ggttactacc agcttaaaat cggctatcac
     4801 ctcggtgatg cggtgctcag tgcgaaagga cagtacaact ggaacaccgg ctacggcggc
     4861 gcggagttag gcttaagtta cccgatcacc aaacatgtgc gcctttatac tcaggtttac
     4921 agcggctatg gcgaatcgct catcgactat aacttcaacc agacccgtgt cggtgtaggg
     4981 gttatgctaa acgacttgtt ttgatgaacg gttgagtggt tggcaaatct ggaatccagc
     5041 atccaggatt accctctcag agactaaaag cattgcagtt tctcgcgcag gcgctgaaaa
     5101 tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg cgcaggcgga agtgttgaat
     5161 ctggagtccg gagctaaaca ggttttacaa gaaacctttg gctaccaaca gtttcgcccc
     5221 ggccaggaag aaattatcga cactgtgctt tccggtcgcg attgcctggt tgttatgccc
     5281 actggtggcg gaaaatccct ttgctatcaa attcctgcct tattgctaaa cggccttacc
     5341 gtggttgttt caccgctgat ttcgttgatg aaagatcagg tggatcaact acaggccaac
     5401 ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg aacagcaact tgaagtgatg
     5461 acaggctgcc gcaccgggca aattcgctta ctgtatatcg ccccggaacg cctgatgctg
     5521 gataactttc ttgagcatct ggcgcactgg aatccggtgt tattagccgt cgatgaagcg
     5581 cactgtatct cccaatgggg ccacgatttc cgcccggaat atgccgcgct cggtcagttg
     5641 cgccagcggt tcccgacgct gccgtttatg gcgctgaccg ccacagccga cgacaccacg
     5701 cgccaggata tcgtgcgcct gctggggctg aacgatccgc tgattcaaat cagcagtttt
     5761 gaccgtccga atattcgcta catgctgatg gagaaattca aaccgctcga tcagttgatg
     5821 cgctacgtgc aggaacagcg cggtaagtct ggcattatct actgcaacag ccgggcgaaa
     5881 gtagaagaca ccgctgcgcg cctgcaaagc aggggtatta gcgcggcggc ctatcatgcc
     5941 gggctggaaa ataatgtccg cgccgacgtg caggagaaat tccagcgcga tgatctgcaa
     6001 attgtggtgg cgacggtggc gttcggcatg ggcatcaata aaccaaacgt tcgcttcgtg
     6061 gtccactttg atattccgcg caatatcgaa tcctattatc aggaaaccgg tcgcgccggg
     6121 cgtgatggcc tgcctgcgga agcgatgctg ttttacgatc cggctgatat ggcgtggctg
     6181 cgccgttgtc tggaagagaa gccgcagggg cagttgcagg atatcgagcg ccacaaactc
     6241 aatgcgatgg gcgcgtttgc cgaagcgcaa acttgccgtc gtctggtatt gctgaactat
     6301 tttggcgaag ggcgtcagga gccgtgcggg aactgcgata tctgcctcga tccgccgaaa
     6361 cagtacgacg gttcaaccga tgctcagatt gccctttcca ccattggtcg tgtgaatcag
     6421 cggtttggga tgggttatgt ggtggaagtg attcgtggtg ctaataacca gcgtatccgc
     6481 gactatggtc atgacaaact gaaagtctat ggtatgggcc gtgataaaag ccatgaacat
     6541 tgggtgagcg tgatccgcca gctgattcat ctcggcctgg tgacgcaaaa tattgcccag
     6601 cattctgccc tacaactgac agaggccgcg cgcccggtgc tgcgcggcga atcctctttg
     6661 caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag cgatgcagaa atcgttcggc
     6721 ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac tgcgtaaatc gattgccgat
     6781 gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa ccttgattga gatggctgaa
     6841 cagatgccga tcaccgccag cgaaatgctc agcgttaacg gcgttgggat gcgcaagctg
     6901 gaacgctttg gtaaaccgtt tatggcgctg attcgtgcgc atgtcgatgg cgacggcgaa
     6961 gagtagtcag cagcataaaa aagtgccagt atgaagactc cgtaaacgtt tcccccgcga
     7021 gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg tgcacattgt ggcgcttatg
     7081 agccccggtc ccgatttctt ttttgtctct cagaccgctg tcagtcgttc ccgtaaagaa
     7141 gcgatgatgg gcgtgctggg cattacctgc ggcgtaatgg tttgggctgg gattgcgttg
     7201 cttggcctgc atttgattat cgaaaaaatg gcctggctgc atacgctgat tatggtaggc
     7261 ggtggcctgt atctctgctg gatgggttac cagatgctac gcggtgcact gaaaaaagag
     7321 gctgtatctg cgcctgcacc acaggttgag ctggcgaaaa gcgggcgcag tttcctgaaa
     7381 ggcttactga ccaatctcgc caatccgaaa gcgattatct attttggttc ggtattctca
     7441 ttgtttgtcg gcgataacgt tggcactacc gagcgctggg gcattttcgc gttgattatt
     7501 gtcgaaacac tggcgtggtt taccgtcgtt gccagtctgt ttgccctgcc gcaaatgcgc
     7561 cgtggttatc aacgtctggc gaagtggatt gatggttttg ccggggcgtt atttgccgga
     7621 tttggcattc atttgattat ttcgcggtga tgccagacgc gtcttcagag taagtcggat
     7681 aaggcgttta cgccgcatcc gacattattt ttcacgcatg cctcgccgat gctaacagcg
     7741 ctcccaccag cataaacaac gagccgaaaa tcttattcag cgccttcatc tgctttggtc
     7801 ctttaatcca tagagcaatc ctttgagcaa gggtggcgta accgatcatc acaataatat
     7861 cgaccacaat agtggtgacg ccgagcacga tatactgcat cagttgcggc tgttgcggca
     7921 tgatgaattg cggaaatagc gccgccagaa acacaatact tttgggattg gtgagattca
     7981 caaaaactgc gcgctggaac aaatgtcgac gcgattgagt agaggccagc gatttaagat
     8041 caattgcgcc agcggcgcgc cactgctgga ttcccagcca aatcaagtaa gccgcgcctg
     8101 cccactttaa cacttcaaac gcaatcactg agcgggaaaa tagcgtcccc aaccccacgc
     8161 caaccagcac aatatgaatc gccagtccgg tctgaagccc agcaatagac gccaccgcgc
     8221 cgcgataacc gtggttgagc gaggtggtca tagtgttgat tgcaccagag cctggcgaca
     8281 ggcttaaaat gatcgatgtc agcaggtagg caaaccacca ttctaaggtc atgatgaact
     8341 cccggtgtgt ctatttttgt gccacaatac gctactgtcg cagcgttgtg tcaggcacgc
     8401 ttaaaaaaac gattttacgt ggtttaagag gcagattacc cgatgtttca gcagcaaaaa
     8461 gactgggaaa caagagaaaa cgcgtttgct gcttttacca tgggaccgct gactgatttc
     8521 tggcgtcagc gtgatgaagc agagtttact ggtgtggatg acattccggt gcgctttgtc
     8581 cgttttcgcg cacagcacca tgaccgggtg gtagtcatct gcccggggcg tattgagagc
     8641 tacgtaaaat atgcggaact ggcctacgac ctgttccatt tggggtttga tgtcttaatc
     8701 atcgaccatc gcgggcaggg acgttccggt cgcctgttag ccgatccgca tctcgggcat
     8761 gttaatcgct ttaatgatta tgttgatgat ctggcggcat tctggcagca ggaggttcag
     8821 cccggtccgt ggcgtaaacg ctatatactg gcacattcga tgggcggtgc gatctccaca
     8881 ttatttctgc aacgccatcc aggtgtatgt gacgccattg cgctaactgc gccaatgttt
     8941 gggatcgtga ttcgtatgcc gtcatttatg gcacggcaga tcctcaactg ggccgaagcg
     9001 catccacgtt tccgtgatgg ctatgcaata ggcaccgggc gctggcgcgc gttgccgttt
     9061 gctatcaacg tactgaccca cagcagacag cgatatcgac gtaacttacg cttctatgct
     9121 gatgacccaa cgattcgcgt cggtgggccg acctaccatt gggtacgcga aagtattctg
     9181 gctggcgaac aggtgttagc cggtgcgggt gatgacgcca cgcaaacgct tctcttacaa
     9241 gctgaagagg aacgcgtggt ggataaccgc atgcacgacc gtttttgtga actccgcacc
     9301 gccgcgggcc atcctgtcga aggaggacgg ccgttggtaa taaaaggtgc ttaccatgag
     9361 atcctttttg aaaaggacgc aatgcgctca gtcgcgctcc acgccatcgt tgattttttc
     9421 aacaggcata actcacccag cggaaaccgc tctacagagg tttaaatttc ttatgtacca
     9481 ggttgttgcg tctgatttag atggcacgtt actttctccc gaccatacgt tatcccctta
     9541 cgccaaagaa actctgaagc tgctcaccgc gcgcggcatc aactttgtgt ttgcgaccgg
     9601 tcgtcaccac gttgatgtgg ggcaaattcg cgataatctg gagattaagt cttacatgat
     9661 tacctccaat ggtgcgcgcg ttcacgatct ggatggcaat ctgatttttg ctcataacct
     9721 ggatcgcgac attgccagcg atctgtttgg cgtagtcaac gacaatccgg acatcattac
     9781 taacgtttat cgcgacgacg aatggtttat gaatcgccat cgcccggaag agatgcgctt
     9841 ttttaaagaa gcggtgttca aatatgcgct gtatgagccg ggattactgg agccggaagg
     9901 cgtcagcaaa gtgtttttca cctgcgattc ccatgaacaa ctgctgccgc tggagcaggc
     9961 gattaacgct cgctggggcg atcgcgtcaa cgtcagtttc tctaccttaa cctgtctgga
    10021 agtgatggcg ggcggcgtgt cgaaaggcca tgcgctggaa gcagtggcga agaaactggg
    10081 ctacagcctg aaggattgta ttgcgtttgg tgacgggatg aacgacgccg aaatgctgtc
    10141 gatggcgggg aaaggctgca ttatgggcag tgcgcaccag cgtctgaaag atctgcatcc
    10201 ggagctggaa gttattggta ctaatgccga cgacgcggtg ccgcattatc tgcgtaaact
    10261 ctatttatcg taatcgttct ttatttggtc agttgtcaac ctgatacttc gctacaatgg
    10321 atactcgtta atcaaagagt tttccattgt ggcgttactt atcatcacca cgattctgtg
    10381 ggccttctcc tttagctttt atggcgagta ccttgcgggg cacgtcgata gctattttgc
    10441 ggtgctggtg cgcgttggct tggcggcact cgtttttctg ccgtttctgc gtacccgtag
    10501 caatagcctg aaaacggtcg gcctgtatat gctggtgggc gcgatgcagc tcggcgtgat
    10561 gtatatgctg agtttccgcg cttatctcta cctgacggtc tccgaattgt tgctgttcac
    10621 cgtactgacg ccgctctaca tcacgctgat ttatgacatc atgagtaagc gccgtctgcg
    10681 ctggggctat gcttttagcg ccttgctggc ggtgattggt gccgggatta ttcgctatga
    10741 tcaggtcacc gaccatttct ggactggctt gttgctggtg caactctcca atatcacttt
    10801 tgccatcggc atggtgggtt acaaacgcct gatggaaact cgcccgatgc cgcagtataa
    10861 cgcctttgcg tggttctatc ttggcgcgtt cctggtggcg gtaattgcat ggttcttgct
    10921 gggaaatgcg cagaaaatgc cgcaaaccac gttgcaatgg ggcattctgg tgtttcttgg
    10981 cgtggtggct tccgggattg gctactttat gtggaactac ggcgcgacgc aggtggacgc
    11041 cggaacgctg ggcattatga ataatatgca cgttccggca gggctgctgg taaacctggc
    11101 tatctggcac caacagccgc actggccaac gtttattaca ggcgcgctgg tgatcctggc
    11161 ctcactgtgg gtgcatcgta agtgggtcgc tccgcgctct tcacaaacgg cagatgatcg
    11221 caggcgtgat tgcgcgctga gcgaataaac gcttccgtaa ctggctgacg ctgctcgcca
    11281 tcgcgcacgg cggcgtacag tcggctccac aagccttcgc ccagggtttt ggtcaccacc
    11341 agaccctggc gctcaaaact ctctactacc caatgcggta gcgcggcaat acccatccgc
    11401 gcggcaacca tttgaatcaa caataaggtg ttatcgacgc ttttcagtga cgggctgacg
    11461 cctgccggct gaagaaaatg ccgccagaca tccagtcgac tacgctgcac cggataaatt
    11521 aatagcgtct cgctggcgag atcttccggt gtaatacgcg tttttgccgc cagtggatgg
    11581 tcaggtgcta acaccaggcg tacttcataa tcgaacatcg gcgaataatg caggccactg
    11641 cgcggcagga tatcggaggt catcaccaga tccagctcgc cctgttgcaa cgcgggctgc
    11701 gggtcaaacg tcacgcccga tttaaaatcc atctctacct gcggccagtt cttatggaaa
    11761 ttttctaaag cgggtgtcag ccactgaata cagctatggc actcaatggc aatgcgcaga
    11821 cgcgtctgct gcggttcatt gcaggcttgc agtgcctggc tgatttgcgg cagcacctgg
    11881 tttgccagtt gcaacaggat ttctccctgc ggtgtaaagc gtagcggctg gctcttacgc
    11941 acaaatagcc ggaagccaag gcgttgttcc agatcgctaa actggtgaga cagggcggat
    12001 tgcgtctgat gcaacgttgc cgcagcggct gcgagcgagc cgcagttccg caacgcttgt
    12061 agcgttttca ggtgttttac ttcgatcatg aaagtccttc acttcggcat gaataatttg
    12121 cgcttgagga atatacagta accgccaatt atggatgtgt aaacatctgg acggctaaaa
    12181 tcattcgtct tttaaattta tggtgcgttg gctgcgtttc tccaccccgg tcacttactt
    12241 cagtaagctc ccggcgatga ataaacttgc cgccttccct aaattcaaaa tccataggat
    12301 ttacatataa ttagaggaag aaaaatgaca atattgaatc acaccctcgg tttccctcgc
    12361 gttggcctgc gtcgcgagct gaaaaaagcg caagaaagtt attgggcggg gaactccacg
    12421 cgtgaagaac tgctgacagt agggcgtgaa ctgcgtgctc gtcactggga tcaacaaaag
    12481 caagcgggta tcgacctgct gccggtgggc gattttgcct ggtacgatca tgtactgacc
    12541 accagtttgc tgctgggtaa tgttccgcca cgtcatcaga acaaagacgg ttcggtagat
    12601 atcgacaccc tgttccgtat tggtcgtgga cgtgcgccga ctggcgaacc tgcggcggca
    12661 gcggaaatga ccaaatggtt taacaccaac tatcactaca tggtgccgga gttcgttaaa
    12721 ggccaacagt tcaaactgac ctggacgcag ctgctggagg aagtggacga ggcgctggcg
    12781 ctgagccaca acgttaagcc tgtgctgctg gggccggtta cttacctgtg gctggggaaa
    12841 gtgaaaggtg aacagtttga ccgccttagc ctgctgaacg acattctgcc ggtttatcag
    12901 caagtgctgg cagaactggc gaaacgcggc atcgagtggg tacagattga tgaacccgcg
    12961 ctggtactgg aactaccaca ggcgtggctg gacgcataca aacccgctta cgacgcgctc
    13021 cagggacagg tgaaactgct gctgaccacc tattttgaag gcgtaacgcc aaatctcgac
    13081 acgattactg cgctgcctgt tcaggggctg catgtggacc ttgtacatgg taaagataac
    13141 gttgttgaac tgcacaagcg cctgccttct gactggctgc tgtctgcggg tctgatcaat
    13201 ggtcgtaacg tctggcgcgc cgatcttact gagaaatatg cgcaaattaa ggacattgtc
    13261 ggcaaacgtg atttgtgggt ggcatcttcc tgctcgttgc tgcacagccc catcgacctg
    13321 agcgtggaaa cgcgtcttga tgcagaagtg aaaagctggt ttgccttcgc cctacaaaaa
    13381 tgccatgaac tggcactgct gcgcgatgcg ctgaacagtg gtgacacggc agctctggca
    13441 gagtggagcg ccccaattca ggcgcgtcgt cactctactc gtgtacataa tccggcggta
    13501 gaaaagcgtc tggcggcgat caccgcccag gacagccagc gtgcgaatgt ctatgaagtg
    13561 cgtgctgaag cccagcgtgc gcgttttaaa ctgccagcgt ggccgaccac cacgattggt
    13621 tccttcccgc aaaccacgga aattcgtacc ctgcgtctgg atttcaaaaa gggcaatctc
    13681 gacgccaaca actaccgcac gggcattgcg gaacatatca agcaggccat tgttgagcag
    13741 gaacgtttgg gactggatgt gctggtacat ggcgaggccg agcgtaatga catggtggaa
    13801 tactttggcg agcacctcga cggatttgtc tttacgcaaa acggttgggt acagagctac
    13861 ggttcccgct gcgtgaagcc accgattgtc attggtgaca ttagccgccc ggcaccgatt
    13921 accgtggagt gggcgaagta tgcgcaatcg ctgaccgaca aaccggtgaa agggatgctg
    13981 acggggccgg tgaccatact ctgctggtcg ttcccgcgtg aagatgtcag ccgtgaaacc
    14041 atcgccaaac agattgcgct ggcgctgcgt gatgaagtgg ccgatctgga agccgctgga
    14101 attggcatca tccagattga cgaaccggcg ctgcgcgaag gtttaccgct gcgtcgtagc
    14161 gactgggatg cgtatctcca gtggggcgta gaggccttcc gtatcaacgc cgccgtggcg
    14221 aaagatgaca cacaaatcca cactcacatg tgttattgcg agttcaacga catcatggat
    14281 tcgattgcgg cgctggacgc agacgtcatc accatcgaaa cctcgcgttc cgacatggag
    14341 ttgctggagt cgtttgaaga gtttgattat ccaaatgaaa tcggtcctgg cgtctatgac
    14401 attcactcgc caaacgtacc gagcgtggaa tggattgaag ccttgctgaa gaaagcggca
    14461 aaacgcattc cggcagagcg cctgtgggtc aacccggact gtggcctgaa aacgcgcggc
    14521 tggccagaaa cccgcgcggc actggcgaac atggtgcagg cggcgcagaa tttgcgtcgg
    14581 gggtaaatcc aaaccgggtg gtaataccac ccggtctttt ctcattacag cgacttcttc
    14641 ccaccatact gcttaaacca ttccagcata cgctgccagc catctttcgc agattcggca
    14701 tgatagctcg ggcgataatc agcgttgaat gcatgcccgg catccgggta cacgataatc
    14761 tctgctttcg cattagcagc ccgcagcgcc tggcgcatcg tttcaacgct ctcctgcgga
    14821 atgctgttat cctgaccacc atataagccg agaaccggcg cgttaagatc ggttgcgata
    14881 tcaacaggtt gtttcggtga attcagcgac ttgtcgcccg tcagtttgcc gtaccacgcc
    14941 actgcggctt ttagctgtgg attatgcgcg gcatacagcc aggtgatacg tccaccccag
    15001 cagaatccgg tgatcattaa acgatgaaca tcgccgccgt tgcgggaagc ccaactggcg
    15061 acatgatcga gatcggccag cacctgcgag tcaggcactt ttgctaccag accgctaagc
    15121 aacgtgggga tatcggcaaa atcattcgga tcgccttcgc ggaagtaaag ttcaggtgcg
    15181 atagccagat acccctccag cgccagacgg cgacaaatgt cgcggatatg ttcatgcacg
    15241 ccaaaaattt cctgcactac aatgaccact ggcagtgggc catcgctttg ctttggtctg
    15301 gcatggtaag caggcatgtt atccccttgt gaagggatag aggtgaagcc cgccacaatc
    15361 gcgtcgtccg gggtctgaac gatggtcgaa gcgagaggcg atgcagcagg tgcaaatcca
    15421 gattgttgtg ttgttgccat ggtattctcc gtacccttat aaaaatgttg cgcaatgtta
    15481 actatagtca gcatgcaaca aatcacattg cctgaatcgg ctcatctttt atgcagtcct
    15541 gcagaatgaa gagtgattta tgtgatatga atcacttttg gtgggtaaat ttatgcaacg
    15601 catttgcgtc atggtgatga gtatcacgaa aaaatgttaa acccttcggt aaagtgtctt
    15661 tttgcttctt ctgactaaac cgattcacag aggagttgta tatgtccaag tctgatgttt
    15721 ttcatctcgg cctcactaaa aacgatttac aaggggctac gcttgccatc gtccctggcg
    15781 acccggatcg tgtggaaaag atcgccgcgc tgatggataa gccggttaag ctggcatctc
    15841 accgcgaatt cactacctgg cgtgcagagc tggatggtaa acctgttatc gtctgctcta
    15901 ccggtatcgg cggcccgtct acctctattg ctgttgaaga gctggcacag ctgggcattc
    15961 gcaccttcct gcgtatcggt acaacgggcg ctattcagcc gcatattaat gtgggtgatg
    16021 tcctggttac cacggcgtct gtccgtctgg atggcgcgag cctgcacttc gcaccgctgg
    16081 aattcccggc tgtcgctgat ttcgaatgta cgactgcgct ggttgaagct gcgaaatcta
    16141 ttggcgcgac aactcacgtt ggcgtgacag tttcttctga taccttctac ccaggtcagg
    16201 aacgttacga tacttactct ggtcgcgtag ttcgtcactt taaaggttct atggaagagt
    16261 ggcaggcgat gggcgtaatg aactatgaaa tggaatctgc aactctgctg accatgtgtg
    16321 caagtcaggg cctgcgtgcc ggtatggtag cgggtgttat cgttaaccgc acccagcaag
    16381 agatcccgaa tgctgagacg atgaaacaaa ccgaaagcca tgcggtgaaa atcgtggtgg
    16441 aagcggcgcg tcgtctgctg taattctctt ctcctgtctg aaggccgacg cgttcggcct
    16501 tttgtatttt tgcgtagcgc ctcgcaggaa atgcctttcc aactggacgt ttgtacagca
    16561 caattctatt ttgtgcgggt aagttgttgc gtcaggaggc gttgtggatt tctcaatcat
    16621 ggtttacgca gttattgcgt tggtgggtgt ggcaattggc tggctgtttg ccagttatca
    16681 acatgcgcag caaaaagccg agcaattagc tgaacgtgaa gagatggtcg cggagttaag
    16741 cgcggcaaaa caacaaatta cccaaagcga gcactggcgt gcagagtgtg aattgctcaa
    16801 taacgaagtg cgcagcctgc aaagtattaa cacctctctg gaggccgatc tgcgtgaagt
    16861 aaccacgcgg atggaagccg cacagcaaca tgctgacgat aaaattcgcc agatgattaa
    16921 cagcgagcag cgcctcagtg agcagtttga aaacctcgcc aaccgtattt ttgagcacag
    16981 caatcgccgg gttgatgagc aaaaccgtca gagtctgaac agcctgttgt cgccgctacg
    17041 tgaacaactg gacggtttcc gccgtcaggt tcaggacagc ttcggtaaag aagcacaaga
    17101 acgccatacc ctgacccacg aaattcgcaa tctccagcaa ctcaacgcgc aaatggccca
    17161 ggaagcgatc aacctgacgc gcgcgctgaa aggcgacaat aaaacccagg gcaactgggg
    17221 cgaggtagta ttgacgcggg tgctggaggc ttccggtctg cgtgaagggt atgaatatga
    17281 aacccaggtc agcatcgaaa atgacgcccg ctcgcggatg cagccggatg tcatcgtgcg
    17341 gctgccgcag ggaaaagatg tggtgatcga cgccaaaatg acgctggtcg cctatgaacg
    17401 ctattttaac gccgaagacg actacacccg cgaaagcgcg ctacaggaac atatcgcgtc
    17461 ggtgcgtaac catatccgtt tgctgggacg caaagattat caacagctgc cggggctgcg
    17521 aactctggat tacgtgctga tgtttattcc cgttgaaccc gcttttttac tggcgcttga
    17581 ccgccagccg gagctgatca ccgaagcgtt gaaaaacaat atcatgctgg ttagcccgac
    17641 tacgctgctg gtggcgttgc gtactattgc caacctgtgg cgttatgagc atcaaagccg
    17701 taacgcccag caaatcgccg atcgtgccag caagctgtac gacaagatgc gtttgttcat
    17761 cgatgacatg tccgcgattg gtcaaagtct cgacaaagcg caggataact accgccaggc
    17821 aatgaaaaaa ctctcttcag gtcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg
    17881 tttaggagta gaaattaaac gcgagattaa tccggatttg gctgaacagg cggtgagcca
    17941 ggatgaagag tatcgacttc ggtcggttcc ggagcagccg aatgatgaag cttatcaacg
    18001 cgatgatgaa tataatcagc agtcgcgcta gcccattagg ggtagttaag ccgggtagaa
    18061 atctagggca tcgacgccca atctgttaca cttctggaac aattttttga tgagcaggca
    18121 ttgagatggt ggataagtca caagaaacga cgcactttgg ttttcagacc gtcgcgaagg
    18181 aacaaaaagc ggatatggtc gcccacgttt tccattccgt ggcatcaaaa tacgatgtca
    18241 tgaatgattt gatgtcattt ggtattcatc gtttgtggaa gcgattcacg attgattgca
    18301 gcggcgtacg ccgtgggcag accgtgctgg atctggctgg tggcaccggc gacctgacag
    18361 cgaaattctc ccgcctggtc ggagaaactg gcaaagtggt ccttgctgat atcaatgaat
    18421 ccatgctcaa aatgggacgc gagaagctgc gtaatatcgg tgtgattggc aacgttgagt
    18481 atgttcaggc gaacgctgag gcgctgccgt tcccggataa cacctttgat tgcatcacca
    18541 tttcgtttgg tctgcgtaac gtcaccgaca aagataaagc actgcgttca atgtatcgcg
    18601 tgctgaaacc cggcggccgc ctgctggtgc ttgagttctc gaagccaatt atcgagccgc
    18661 tgagcaaagc ctatgatgca tactccttcc atgtgctgcc gcgtattggc tcactggtcg
    18721 cgaacgacgc cgacagctac cgttatctgg cagaatccat ccgtatgcat cccgatcagg
    18781 ataccctgaa agccatgatg caggatgccg gattcgaaag tgtcgactac tacaatctga
    18841 cggcaggggt tgtggcgctg catcgtggtt ataagttctg acaggagacc ggaaatgcct
    18901 tttaaacctt tagtgacggc aggaattgaa agtctgctca acaccttcct gtatcgctca
    18961 cccgcgctga aaacagcccg ctcgcgtctg ctgggtaaag tattgcgcgt ggaggtaaaa
    19021 ggcttttcga cgtcattgat tctggtgttc agcgaacgcc aggttgatgt actgggcgaa
    19081 tgggcaggcg atgctgactg caccgttatc gcctacgcca gtgtgttgcc gaaacttcgc
    19141 gatcgccagc agcttaccgc actgattcgc agtggtgagc tggaagtgca gggcgatatt
    19201 caggtggtgc aaaacttcgt tgcgctggca gatctggcag agttcgaccc tgcggaactg
    19261 ctggcccctt ataccggtga tatcgccgct gaaggaatca gcaaagccat gcgcggaggc
    19321 gcaaagttcc tgcatcacgg cattaagcgc cagcaacgtt atgtggcgga agccattact
    19381 gaagagtggc gtatggcacc cggtccgctt gaagtggcct ggtttgcgga agagacggct
    19441 gccgtcgagc gtgctgttga tgccctgacc aaacggctgg aaaaactgga ggctaaatga
    19501 cgccaggtga agtacggcgc ctatatttca tcattcgcac ttttttaagc tacggacttg
    19561 atgaactgat ccccaaaatg cgtatcaccc tgccgctacg gctatggcga tactcattat
    19621 tctggatgcc aaatcggcat aaagacaaac ttttaggtga gcgactacga ctggccctgc
    19681 aagaactggg gccggtttgg atcaagttcg ggcaaatgtt atcaacccgc cgcgatcttt
    19741 ttccaccgca tattgccgat cagctggcgt tattgcagga caaagttgct ccgtttgatg
    19801 gcaagctggc gaagcagcag attgaagctg caatgggcgg cttgccggta gaagcgtggt
    19861 ttgacgattt tgaaatcaag ccgctggctt ctgcttctat cgcccaggtt cataccgcgc
    19921 gattgaaatc gaatggtaaa gaggtggtga ttaaagtcat ccgcccggat attttgccgg
    19981 tcattaaagc ggatctgaaa cttatctacc gtctggctcg ctgggtgccg cgtttgctgc
    20041 cggatggtcg ccgtctgcgc ccaaccgaag tggtgcgcga gtacgaaaag accttgattg
    20101 atgaactgaa tttgctgcgg gaatctgcca acgccattca gcttcggcgc aattttgaag
    20161 acagcccgat gctctacatc ccggaagttt accctgacta ttgtagtgaa gggatgatgg
    20221 tgatggagcg catttacggc attccggtgt ctgatgttgc ggcgctggag aaaaacggca
    20281 ctaacatgaa attgctggcg gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg
    20341 acagcttttt ccatgccgat atgcaccctg gcaacatctt cgtaagctat gaacacccgg
    20401 aaaacccgaa atatatcggc attgattgcg ggattgttgg ctcgctaaac aaagaagata
    20461 aacgctatct ggcagaaaac tttatcgcct tctttaatcg cgactatcgc aaagtggcag
    20521 agctacacgt cgattctggc tgggtgccac cagataccaa cgttgaagag ttcgaatttg
    20581 ccattcgtac ggtctgtgaa cctatctttg agaaaccgct ggccgaaatt tcgtttggac
    20641 atgtactgtt aaatctgttt aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac
    20701 tggtgttact ccagaaaacc ctgctctatg tcgaaggggt aggacgccag ctttatccgc
    20761 agctcgattt atggaaaaca gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg
    20821 gtattcctgc cctggtgaga gcatttaaag aaaaagcgcc gttctgggtc gaaaaaatgc
    20881 cagaactgcc tgaactggtt tacgacagtt tgcgccaggg caagtattta cagcacagtg
    20941 ttgataagat tgcccgcgag cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt
    21001 ttctcggaat tggcgctacg ttagtattaa gtggcacatt cttgttggtc agccgacctg
    21061 agtgggggct gatgcccggc tggttaatgg caggtggtct gatcgcctgg tttgtcggtt
    21121 ggcgcaaaac acgctgattt tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc
    21181 tttgtttaat catcatctac cacagaggaa catgtatggg tggtatcagt atttggcagt
    21241 tattgattat tgccgtcatc gttgtactgc tttttggcac caaaaagctc ggctccatcg
    21301 gttccgatct tggtgcgtcg atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa
    21361 agcaggataa aaccagtcag gatgctgatt ttactgcgaa aactatcgcc gataagcagg
    21421 cggatacgaa tcaggaacag gctaaaacag aagacgcgaa gcgccacgat aaagagcagg
    21481 tgtaatccgt gtttgatatc ggttttagcg aactgctatt ggtgttcatc atcggcctcg
    21541 tcgttctggg gccgcaacga ctgcctgtgg cggtaaaaac ggtagcgggc tggattcgcg
    21601 cgttgcgttc actggcgaca acggtgcaga acgaactgac ccaggagtta aaactccagg
    21661 agtttcagga cagtctgaaa aaggttgaaa aggcgagcct cactaacctg acgcccgaac
    21721 tgaaagcgtc gatggatgaa ctacgccagg ccgcggagtc gatgaagcgt tcctacgttg
    21781 caaacgatcc tgaaaaggcg agcgatgaag cgcacaccat ccataacccg gtggtgaaag
    21841 ataatgaagc tgcgcatgag ggcgtaacgc ctgccgctgc acaaacgcag gccagttcgc
    21901 cggaacagaa gccagaaacc acgccagagc cggtggtaaa acctgctgcg gacgctgaac
    21961 cgaaaaccgc tgcaccttcc ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga
    22021 tactcaaccg cttatcacgc atctgattga gctgcgtaag cgtctgctga actgcattat
    22081 cgcggtgatc gtgatattcc tgtgtctggt ctatttcgcc aatgacatct atcacttggt
    22141 atccgcgcca ttgatcaagc agttgccgca aggttcaacg atgatcgcca ccgacgtggc
    22201 ctcgccgttc tttacgccga tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc
    22261 ggtgattctc tatcaggtgt gggcatttat cgccccagcg ctgtataagc atgaacgtcg
    22321 cctggtggtg ccgctgctgg tttccagctc tctgctgttt tatatcggca tggcattcgc
    22381 ctactttgtg gtctttccgc tggcatttgg cttccttgcc aataccgcgc cggaaggggt
    22441 gcaggtatcc accgacatcg ccagctattt aagcttcgtt atggcgctgt ttatggcgtt
    22501 tggtgtctcc tttgaagtgc cggtggcaat tgtgctgctg tgctggatgg ggattacctc
    22561 gtcagaagac ttacgcaaaa aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat
    22621 gttgctgacg ccgccggatg tcttctcgca aacgctattg gcgatcccga tgtactgtct
    22681 gtttgaaatc ggtgtcttct tctcacgctt ttacgttggt aaagggcgaa atcgggaaga
    22741 ggaaaacgac gctgaagcag aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag
    22801 ggcggttgtc atatggagta caggatgttt gatatcggcg ttaatttgac cagttcgcaa
    22861 tttgcgaaag accgtgatga tgttgtagcg cgcgcttttg acgcgggagt taatgggcta
    22921 ctcatcaccg gcactaacct gcgtgaaagc cagcaggcgc aaaagctggc gcgtcagtat
    22981 tcgtcctgtt ggtcaacggc gggcgtacat cctcacgaca gcagccagtg gcaagctgcg
    23041 actgaagaag cgattattga gctggccgcg cagccagaag tggtggcgat tggtgaatgt
    23101 ggtctcgact ttaaccgcaa cttttcgacg ccggaagagc aggaacgcgc ttttgttgct
    23161 cagctacgca ttgccgcaga attaaacatg ccggtattta tgcactgtcg cgatgcccac
    23221 gagcggttta tgacattgct ggagccgtgg ctggataaac tgcctggtgc ggttcttcat
    23281 tgctttaccg gcacacgcga agagatgcag gcgtgcgtgg cgcgtggaat ttatatcggc
    23341 attaccggtt gggtttgcga tgaacgacgc gggctggagc tgcgggaatt gttgccgttg
    23401 attccggcgg agaaattgct gatcgaaaca gatgcgccgt atctgctccc gcgcgatctc
    23461 acgccaaagc catcatcccg gcgcaacgag ccagcccatc tgccccatat tttgcaacgt
    23521 attgcgcact ggcgtggaga agatgccgca tggctggctg ccaccacgga tgccaatgtc
    23581 aaaacactgt ttgggattgc gttttagagt ttgcggaact cggtgttctt cacactgtgc
    23641 ttaatctctt tattaataag attaagcaat agcatggagc gagcctcacc atcgggttcg
    23701 gtgaaaatgg cctgaaagcc ttcgaacgcg ccttcggtaa taatcacctt atcacccgga
    23761 taaggggggg ccggatcgac aatgtctttc ggtttatata ccgatagctg atgaattacc
    23821 gccgatggga ctatcgctgg cgacgcgcca aagcgcacaa agtggctgac gccacgcgta
    23881 gcgctgatgg tcgtggtatg aatcacttct ggatcaaatt ccacaaacag gtagttaggg
    23941 aataacggtt cactgactgc ggtacgttta ccccgtacga ttttttccag ggtgatcatc
    24001 ggagcgaggc aattaaccgc ctgtctctca aggtgttcct gggcacgttg aagttgcccg
    24061 cgcttgcagt acagtaaata ccaggattgc ataatgactc ttatccgttt aatcggggcg
    24121 caaggatagc aaaagcttta cgctaagtta attatattcc ccggtttgcg ttataccgtc
    24181 agagttcacg ctaatttaac aaatttacag cattgcaaag atgaacgccg tataatgggc
    24241 gcagattaag aggctacaat ggacgccatg aaatataacg atttacgcga cttcctgacg
    24301 ctgcttgaac agcagggtga gctaaaacgt atcacgctcc cggtggatcc gcatctggaa
    24361 atcactgaaa ttgctgaccg cactctgcgt gctggtgggc ctgcgctgtt gttcgaaaac
    24421 cctaaaggct actcaatgcc ggtgctgtgc aacctgttcg gtacgccaaa gcgcgtggcg
    24481 atgggcatgg ggcaggaaga tgtttcggcg ctgcgtgaag ttggtaaatt attggcgttt
    24541 ctgaaagagc cggagccgcc aaaaggtttc cgcgacctgt ttgataaact gccgcagttt
    24601 aagcaggtgt taaacatgcc gacaaagcga ctgcgtggtg cgccctgcca acaaaaaatc
    24661 gtctctggcg atgacgtcga tctcaatcgc attcccatta tgacctgctg gccggaagat
    24721 gccgcgccgc tgattacctg ggggctgacc gtaacgcgcg gcccgcataa agagcggcag
    24781 aatctgggca tttatcgcca gcagctgatt ggtaaaaaca aactgattat gcgctggctg
    24841 tcgcatcgcg gcggcgcgct ggattatcag gagtggtgtg cggcgcatcc gggcgaacat
    24901 ttcccggttt ctgtggcgct gggtgccgat cctgccacga ttctcggtgc agtcaccccc
    24961 gttccggata cgctttcaga gtatgcgttt gccggattgc tacgcggcac caaaaccgaa
    25021 gtagtaaagt gtatttccaa tgacctcgaa gtgccagcca gtgccgaaat cgtcctggaa
    25081 gggtatatcg atcctggtga gatggcgccg gaagggccgt atggtgacca cacaggttac
    25141 tataatgaag tcgatagttt cccggtgttt accgtgacgc atattaccca gcgtgaagat
    25201 gcgatttacc attccaccta taccgggcgt ccgccagatg agcccgcggt actgggcgtg
    25261 gcgttgaacg aagtgtttgt accgattctg caaaaacagt tcccggaaat tgtcgatttt
    25321 tacctgccgc cggaaggctg ttcttatcgt ctggcggtag tgacgatcaa aaaacagtac
    25381 gccggacacg cgaagcgcgt catgatgggc gtctggtcgt tcttacgcca gtttatgtac
    25441 actaaatttg tgatcgtttg cgatgatgac gtcaacgcac gcgactggaa cgatgtgatt
    25501 tgggcgatta ccacccgtat ggacccggcg cgggatactg ttctggtaga aaatacgcct
    25561 attgattatc tggattttgc ctcgcctgtc tctgggctgg gttcaaaaat ggggctggat
    25621 gccacgaata aatggccggg ggaaacccag cgtgaatggg gacgtcccat caaaaaagat
    25681 ccagatgttg tcgcgcatat tgacgccatc tgggatgaac tggctatttt taacaacggt
    25741 aaaagcgcct gatgcgcgtt tgttttgccc tatttatcga tccgacagag aaagcgcatg
    25801 acaaccttaa gctgtaaagt gacctcggta gcagctatca cggataccgt atatcgtgtc
    25861 cgcatcgtgc cagacgcggc cttttctttt cgtgctggtc agtatttgat ggtagtgatg
    25921 gatgagcgcg acaaacgtcc gttctcaatg gcctcgacgc cggatgaaaa agggtttatc
    25981 gagctgcata ttggcgcttc tgaaatcaac ctttacgcga aagcagtcat ggaccgcatc
    26041 ctcaaagatc atcaaatcgt ggtcgacctt ccccacggag aagcgtggct gcgcgatgat
    26101 gaagagcgtc cgatgatttt gattgcgggc ggcaccgggt tctcttatgc ccgctcgatt
    26161 ttgctgacag cgttggcgcg taacccaaac cgtgatatca ccatttactg gggcgggcgt
    26221 gaagagcagc atctgtatga tctctgcgag cttgaggcgc tttcgttaaa gcaccttggt
    26281 ctgcaagtgg tgccggtggt tgaacaaccg gaagcaggct ggcgtgggcg tactggtacc
    26341 gtgttaacgg cggtattgca ggatcatggt acgctggcag agcatgatat ctatattgcc
    26401 ggacgttttg agatggcgaa aattgcccgt gacctgtttt gcagtgagcg taatgcgcgg
    26461 gaagatcgcc tgtttggcga tgcgtttgca tttatctgag atataaaaaa acccgcccct
    26521 gacaggcggg aagaacggca actaaaactt tattcagtgg catttagatc tatgtcgcat
    26581 ccggcaattg gtgcacgatg cctgatgcga cgctggcgcg tcttatcagg cctacattgg
    26641 tgccggatcg gtagaccgga taaggcgttc acgccgcatc cggcaagtgg ttaaacccgc
    26701 tcaaacaccg tcgcaatacc ctgacccaga ccgatacaca tcgtcgccag accaaactga
    26761 acgtctttgc gttccatcag attcagcagc gtggtgctga tacgcgcacc ggaacaaccc
    26821 agcggatgac ccagcgcgat cgcaccacca ttgaggttga tcttctcgtc aatctgctcc
    26881 attagcccga gatctttaat gcacggcagg atctgcgcgg caaaggcttc gttcatctca
    26941 aacacgccga tatcgctggc agaaagcccc gcttttttca gcgccagctt cgaggccgga
    27001 accgggccgt aacccataat cgatgggtca caaccaacga ccgccatcga acgcacacga
    27061 gcgcgcggct taagacctaa ttcatgggcg cggctttcac tcatcaccag catggcagct
    27121 gcgccatcgg aaagtgcaga agatgtgccc gccgttaccg taccgtttac tggatcaaac
    27181 gccggacgca gcgtggcgag ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa
    27241 ttaaactgct tcaggacgcc gtcggcatcg tgaccaccag ttgggatgat ttcattttta
    27301 aatgcggccg actgcgtggc ggcccaggcg cgggcgtgtg accgcgcggc aaaggcatcc
    27361 tgcatttcac ggctgatacc gtgcatacgc gccagcattt ctgccgttaa gcccatcatg
    27421 cccgccgctt tggcgacatt gcggctcagg ccggggtgaa aatcgacgcc gtgactcatc
    27481 ggcacatggc ctatatgctc cacgccgcca accagacatg cctgcgcatc gccagtcatg
    27541 atcattcgtg ctgcgtcatg cagtgcctgc atggatgaac cacacaagcg attgacggta
    27601 accgccggga cagagtgcgg cacttctgcc agcagcgccg cgttacgggc gatgttaaaa
    27661 ccctgctcca gcgtctgttg cacacaaccc cagtaaatat cgtcgagggc cgccgcttcc
    27721 agcgccgggt tacgcgccag caggctacgc attaaatgag cggagagatc ttccgcacgc
    27781 acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg
    27841 acaacctgtt ccattgtgac tccttaagcc gttttcaggt cgccaaccgg acgggctggc
    27901 tcaaccggag gatagtacgg ttcgttatga cgcgctttat tacgcagacc ttccggcact
    27961 tcatacagcg ggccgaggtg ctgatattgc tgtgccatat cgaggtattt tgcgctaccg
    28021 agggtgtcca gccagcggaa cgcgccgccg tggaacggag ggaagcccag gccgtagacc
    28081 agcgccatat ccgcttccgc cggagtggcg ataatgccct cttccagaca gcgcaccact
    28141 tcgttgacca tcgggatcat catgcgggcg ataatctctt cttcgctgaa atcgcgcttc
    28201 ggttggctca cttctgccag caggtcttca acggcggcgt cttcttcttt cttcggctta
    28261 cctttgctgt cttctttata acgccagaaa ccgaggccgt tcttctgacc aaagcggttg
    28321 gcatcaaaca gcgcgtcgat ggcatcgcgg taatctttct gcatccgctg cgggaagcct
    28381 gctgccatga cagcctgagc gtgatgcgcg gtatcaatgc ccacaacgtc cagcagatat
    28441 gccgggccca tcggccagcc aaactgtttt tccatcactt tgtcgatctt gcggaaatcc
    28501 gcgccgtcgc gcagcagctg gctgaaaccg gcgaaatacg ggaacagcac gcggttaaca
    28561 aagaagccgg ggcagtcgtt aaccacaatc ggcgtcttgc ccatcttgct cgcccaggcg
    28621 acaactttcg cgatggtttc gtcggagctt ttctcgccgc gaataatttc taccaacggc
    28681 attcggtgga ccgggttaaa gaagtgcatc ccgcagaagt tttccgggcg ttccagcgcg
    28741 ttggccagtt cgctgatagg aatggttgaa gtgttagacg ccagcacggt atcctggcgt
    28801 actttttgtt cggtttctgc cagtacggct tttttcactt tcgggttttc aacaaccgct
    28861 tctaccacaa tatccacgcg gtcaaatccg gcgtagtcga gcgttgggtg gatcgtggag
    28921 atcacgccag ccagtttcag accatcgatt ttgccgcgct caagctgctt gttcagcagt
    28981 ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt tgatatcttt catgacaacc
    29041 ggcacgcctt tccacgcgga ctggtaagcg ataccgccac ccatgatgcc tgcgcccagc
    29101 acagcagcct gtttcggggt ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca
    29161 tattgatcgt taaggaaaat gccgaccagt gcgcgagctt cgttggtatg cgccagcggg
    29221 acaaaacttt tgttttccag atttaaggct tcttcacgac caaaacgggc cgcagcttca
    29281 atggttttta ctgcggtgat gggggccgga taatgtttcc ccgccgtttg tgcgaccatt
    29341 cctttagcga tggtgaagct catggtggct tcaatcttgc tcagttttag cggttcctgc
    29401 ttcggctgac gtttcgcttt ccagtcgagg tcgccgttaa tggcctggcg taaaatcgcc
    29461 atcgcccctt caatcagttt ttctgctttg actacgccat ccaccagacc gattttcagc
    29521 gcctgatccg cgccgacatc tttaccggcg gcaatgattt ccagtgcact gtcagcgccc
    29581 agcatacgtg gcatacgtac agaaccgcca aagcccggca tgatgcccag tttggtttcc
    29641 ggcagaccga tgcgcagatc cggcgtcgcc agacgataat cggtcgccag cacgcattcg
    29701 cagccaccac ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg cagatcttcc
    29761 aggcgattaa acacgctatt ggcaaagtgc agccactgac ttaactgttc ttcaggaacg
    29821 aggaacaggg acaaaaattc ggtgatatca gcaccgacga taaaggctgc tttgttcgaa
    29881 cgcagcagca gcccttttag atctgattgc tgttccagca cgccgatggc ctcgccgagg
    29941 ctggcgacgg tcgcagtgtc gagtttatta actgaacctg gggcatcaaa taccagttcg
    30001 gcaatgccat cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc
    30061 tcctgaatcc gcaaggtgat ctggtcgtac cagatgcatc ggagtgtgtg ttttgtgtta
    30121 aaaatatgca aataaaagat taaagaaatg ccgatctgat cacgctcggc agaaatcacg
    30181 ctctggatga acgatgtgct aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa
    30241 aaatggaatc actggcctcg ctctataaaa atcatatagc taccttacaa gaacggactc
    30301 gcgatgcgct ggcgcgcttc aagctggatg cattacttat tcactccggc gagctgttca
    30361 acgtttttct cgacgatcat ccctatccgt ttaaagtgaa cccgcaattc aaagcgtggg
    30421 tgccggtaac tcaggtgcca aactgctggt tgctggtgga tggcgtgaac aagccgaaac
    30481 tgtggttcta tctgccggtt gattactggc acaacgtcga accgctgccg acctccttct
    30541 ggactgaaga tgtggaagtg atcgcgctgc cgaaagccga tggcattggt agcctgctgc
    30601 ctgctgcgcg cggcaatatc ggttatatcg gtccggtgcc ggaacgtgcg ctgcaactgg
    30661 gtattgaggc cagcaacatc aacccgaaag gggtgatcga ctacctgcat tattaccgct
    30721 ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaatg
    30781 gtcatcgtgc ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat atcaatattg
    30841 cctatctgac cgcgaccggt catcgtgata ccgacgtacc ttacagcaac attgtggcgc
    30901 ttaacgaaca cgctgcggtg ttgcattaca ccaaactgga ccaccaggca ccggaagaga
    30961 tgcgcagctt cctgctggat gccggggccg aatataacgg ctatgcggct gacctgaccc
    31021 gtacctggtc ggcaaaaagc gacaacgact acgcacagct ggtgaaagac gtaaatgatg
    31081 aacaactggc gctgatcgcc accatgaaag caggcgtcag ctatgtggat taccacatcc
    31141 agttccatca gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc gatatgagtg
    31201 aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt attggccatc
    31261 cgctgggcct gcaggtgcat gacgtcgccg gttttatgca ggatgatagc ggtacacacc
    31321 tcgcggcacc ggcaaaatat ccgtacctgc gctacacccg tattctccag ccgggcatgg
    31381 tgttaaccat cgaaccgggt atctacttca ttgaatcgct tctggcaccg tggcgtgaag
    31441 ggcagttcag caagcacttc aactggcaga aaattgaagc actgaaaccg ttcggcggca
    31501 ttcgtatcga agacaacgtg gtgatccacg aaaacaacgt ggaaaacatg acccgggatc
    31561 tgaaactggc gtgatggaaa gctggttaat tcctgcggca ccggtcacgg tcgttgaaga
    31621 gatcaaaaag agccgcttca taacgatgtt ggcgcatacc gatggcgttg aggcggcgaa
    31681 agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc cgccaccatt gcgtggcgtg
    31741 ggtggcgggt gcgccggatg attctcaaca gctgggtttc tctgacgacg gggagccggc
    31801 gggaacggca ggtaaaccga tgctcgccca gctaatgggc agcggcgtcg gggaaattac
    31861 cgctgtggta gtgcgctact acggcggcat attgcttggc accggtgggt tagtgaaagc
    31921 gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca agacgccatt
    31981 aaccgaatat actttgcaat gtgaatatag tcagttaacc ggcattgaag cgttgctggg
    32041 gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc
    32101 gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta gccgtggttc
    32161 attgcaattg ttagcgattg aagaataatc cccacttcgt tttgcagaac taaggaagcg
    32221 gcagagatgc attttcgcgc cattacccga atcgttggac tactggtcat cttattttca
    32281 gggaccatga ttatccctgg gctggtagca ctcatctacc gggatggagc gggccgcgct
    32341 tttacccaga ccttttttgt cgccctcgcc attggctcta tgctgtggtg gccgaaccgc
    32401 aaagagaagg gcgaactgaa atcccgtgag gggtttctga tagtggtgct gttttggacc
    32461 gtgctgggta gtgtcggtgc gctccctttt atcttctcgg aaagcccgaa cctcacgatt
    32521 accgatgcgt tttttgaatc tttctctggc ctgaccacca ccggggccac tacgctggtg
    32581 gggctggatt cgctccctca tgccatcctc ttttatcgcc agatgctgca atggtttggc
    32641 gggatgggga tcatcgtgtt agcggttgcg atactgccta tcctcggcgt gggtgggatg
    32701 cagctctatc gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt
    32761 gcggaaacgg cgaaaactct gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg
    32821 gcgttgtggt ttgccggaat ggatgccttt gatgccatcg gccatagctt tgcgactatc
    32881 gctattggcg gcttctcgac acatgatgcc agtatcggtt atttcgacag tccgactatt
    32941 aacactatca ttgctatctt cctgctgatc tccggctgta actacggtct gcacttttca
    33001 ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg catgtttatc
    33061 ggcgtacagt ttacgctggt ggttatttgt acactcgtac tgtggtttca taatgtctac
    33121 agttcggcgc tgatgacaat taaccaggcg tttttccagg tggtatcgat ggcgacaacc
    33181 gccgggttta cgactgacag cattgcccgc tggccgctct ttttgccggt actgctttta
    33241 tgttcagctt ttatcggcgg ttgcgccggg tcaacgggcg gtggcctgaa agtgatccgc
    33301 atcctgctgc tgtttaagca ggggaaccgt gagctgaaac gactggtgca tccgaacgcc
    33361 gtctatagca ttaagctggg gaatcgcgca ctgccggaac gtatcctcga agccgtttgg
    33421 ggatttttct ccgcctatgc attggtgttt attgtcagta tgctggcgat tatcgccacg
    33481 ggcgtggatg atttttctgc ctttgcctca gttgttgcga cattgaataa cctggggcca
    33541 gggcttggcg tggttgctga taactttacc agtatgaacc cggtggctaa atggatcctg
    33601 attgccaaca tgctgtttgg tcgtctcgag gtctttacat tactggtgct ctttaccccg
    33661 actttctggc gtgaatgatg gagtaatacg tgaaaacatt aattcttttc tcaacaaggg
    33721 acggacaaac gcgcgagatt gcctcctacc tggcttcgga actgaaagaa ctggggatcc
    33781 agacggatgt cgccaatgtg caccgcattg aagaaccaca atgggaaaac tatgaccgtg
    33841 tggtcattgg tgcttctatt cgctatggtc actaccactc tgcgttccag gaatttgtca
    33901 aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt ttattccgta aacctggtgg
    33961 cgcgcaaacc ggagaagcgt actccacaga ccaacagcta cgcgcggaag tttctgatga
    34021 actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt tacccacgtt
    34081 atcgctggta cgaccgtttt atgctcaagc tgattatgaa gatgtcaggc ggtgaaacgg
    34141 atacgcgcaa agaagttgtc tataccgatt gggagcaggt ggcgaatttc gcccgagaaa
    34201 tcgcccattt aaccgacaaa ccgacgctga aataagcata aagaataaaa aatgcgcggt
    34261 cagaaaatta ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc
    34321 actgacacgg aacaacggca aacacgccgc cgggtcagcg gggttctcct gagaattccg
    34381 acagagaaag caaaaataaa tgcttgactc tgtagcggga aagcgtatta tgaacactct
    34441 gcgc
//
LOCUS       DAEEGA010000038        34090 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEEGA010000038 DAEEGA010000000
VERSION     DAEEGA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34090
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.038"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..578
                     /locus_tag="KLH46_001976"
     CDS             <1..578
                     /locus_tag="KLH46_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0378669.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREILHQLAQSQQLRPEQAAESED"
     gene            complement(575..1711)
                     /locus_tag="KLH46_001977"
     CDS             complement(575..1711)
                     /locus_tag="KLH46_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE0378670.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1717..2040)
                     /locus_tag="KLH46_001978"
     CDS             complement(1717..2040)
                     /locus_tag="KLH46_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBE0378671.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2223..2366
                     /locus_tag="KLH46_001979"
     CDS             2223..2366
                     /locus_tag="KLH46_001979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378672.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="KLH46_001980"
     CDS             complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="KLH46_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HBE0378673.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="KLH46_001981"
     CDS             complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="KLH46_001981"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBE0378674.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(5629..7473)
                     /gene="selB"
                     /locus_tag="KLH46_001982"
     CDS             complement(5629..7473)
                     /gene="selB"
                     /locus_tag="KLH46_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBE0378675.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7470..8861)
                     /gene="selA"
                     /locus_tag="KLH46_001983"
     CDS             complement(7470..8861)
                     /gene="selA"
                     /locus_tag="KLH46_001983"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBE0378676.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8959..9567)
                     /locus_tag="KLH46_001984"
     CDS             complement(8959..9567)
                     /locus_tag="KLH46_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBE0378677.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9638..10723)
                     /locus_tag="KLH46_001985"
     CDS             complement(9638..10723)
                     /locus_tag="KLH46_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE0378678.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNSFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10726..11088)
                     /locus_tag="KLH46_001986"
     CDS             complement(10726..11088)
                     /locus_tag="KLH46_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBE0378679.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11625..13538
                     /gene="mtlA"
                     /locus_tag="KLH46_001987"
     CDS             11625..13538
                     /gene="mtlA"
                     /locus_tag="KLH46_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBE0378680.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            13633..14781
                     /gene="mtlD"
                     /locus_tag="KLH46_001988"
     CDS             13633..14781
                     /gene="mtlD"
                     /locus_tag="KLH46_001988"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBE0378681.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14781..15368
                     /gene="mtlR"
                     /locus_tag="KLH46_001989"
     CDS             14781..15368
                     /gene="mtlR"
                     /locus_tag="KLH46_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBE0378682.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(15380..15589)
                     /locus_tag="KLH46_001990"
     CDS             complement(15380..15589)
                     /locus_tag="KLH46_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378683.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            15873..16235
                     /locus_tag="KLH46_001991"
     CDS             15873..16235
                     /locus_tag="KLH46_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBE0378684.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16779..17462
                     /locus_tag="KLH46_001992"
     CDS             16779..17462
                     /locus_tag="KLH46_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBE0378685.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            17506..21871
                     /locus_tag="KLH46_001993"
                     /pseudo
     CDS             17506..21871
                     /locus_tag="KLH46_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            22239..23894
                     /gene="lldP"
                     /locus_tag="KLH46_001994"
     CDS             22239..23894
                     /gene="lldP"
                     /locus_tag="KLH46_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBE0378686.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            23894..24670
                     /gene="lldR"
                     /locus_tag="KLH46_001995"
     CDS             23894..24670
                     /gene="lldR"
                     /locus_tag="KLH46_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBE0378687.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24667..25857
                     /gene="lldD"
                     /locus_tag="KLH46_001996"
     CDS             24667..25857
                     /gene="lldD"
                     /locus_tag="KLH46_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBE0378688.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTILLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            26053..26526
                     /gene="trmL"
                     /locus_tag="KLH46_001997"
     CDS             26053..26526
                     /gene="trmL"
                     /locus_tag="KLH46_001997"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBE0378689.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26579..27400)
                     /gene="cysE"
                     /locus_tag="KLH46_001998"
     CDS             complement(26579..27400)
                     /gene="cysE"
                     /locus_tag="KLH46_001998"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBE0378690.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27480..28499)
                     /gene="gpsA"
                     /locus_tag="KLH46_001999"
     CDS             complement(27480..28499)
                     /gene="gpsA"
                     /locus_tag="KLH46_001999"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBE0378691.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28499..28966)
                     /gene="secB"
                     /locus_tag="KLH46_002000"
     CDS             complement(28499..28966)
                     /gene="secB"
                     /locus_tag="KLH46_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBE0378692.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(29029..29280)
                     /gene="grxC"
                     /locus_tag="KLH46_002001"
     CDS             complement(29029..29280)
                     /gene="grxC"
                     /locus_tag="KLH46_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBE0378693.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29422..29853)
                     /locus_tag="KLH46_002002"
     CDS             complement(29422..29853)
                     /locus_tag="KLH46_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBE0378694.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            30098..31642
                     /gene="gpmM"
                     /locus_tag="KLH46_002003"
                     /gene_synonym="pgmI"
     CDS             30098..31642
                     /gene="gpmM"
                     /locus_tag="KLH46_002003"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBE0378695.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31652..32935
                     /gene="envC"
                     /locus_tag="KLH46_002004"
     CDS             31652..32935
                     /gene="envC"
                     /locus_tag="KLH46_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBE0378696.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            32939..33898
                     /locus_tag="KLH46_002005"
     CDS             32939..33898
                     /locus_tag="KLH46_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBE0378697.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(33885..34090)
                     /locus_tag="KLH46_002006"
                     /pseudo
     CDS             complement(33885..34090)
                     /locus_tag="KLH46_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         8470 a         8932 c         8690 g         7998 t
ORIGIN      
        1 ttgagcgtaa tgccttttat gaaggcgtcg aatttcctga tgattgtgat gttttaatca
       61 gctacgctcc accaaaagat gaatcgctgg ttgccagttt tattacacaa tatgccgtaa
      121 ccgcttatgc cagccagcgc tatcttgaga aacatcctat tagccgtcct gacgaactcg
      181 aacatcactc ctgtattttg atcgactcga tgatgatcga cgacgcgaat atctggcgct
      241 tcaacgtcgc cgggagtaaa gaggtgcggg attatcgcgt gaagggaaat tacgtttgcg
      301 ataacacgca atcggcgctg gagctggcac gaaatcacct ggggattgtg tttgcgccag
      361 acaaaagtgt gcagagcgat ctgcaagacg gcacgctggt gccctgcttt cagcacccgt
      421 atgaatggtg gctggatctg gtggctatct tccgtaaacg ggaatacctg ccctggcggg
      481 tccagtatgt gctggatgaa atgctgcgtg aaattctcca ccagcttgct cagtcgcagc
      541 aactgcggcc tgaacaagcg gcggaaagcg aagattaatg atcgagataa aggtaatgca
      601 cccagctggt catgcgtaat agcactttgc gcatcaccga gacatggctg aaatgatcag
      661 agtagaccgc cacctgggcg taaataccgt ctggtaaagc gctcaaatca gtgtgctcat
      721 ccagttcaat ggtcgcgata acgccatctg aacccggcgc tgtgtttaac gtctgtaagg
      781 tgccggtcga ctgataagct ccgccgggaa cagcaggact aatggctgcc agcttgccgc
      841 tgaatacctt acctggcaga gcattaaaca ccacttccgc atcgtcgcca ggggccagtc
      901 gcagcaagga gttctgacgg aactgcgcca caatttgtcg tttctgatcg ggtataaaca
      961 ccatcaccgg acgtagcggc agcgacgcgg cataggtacc cggacgaatc agcacctggg
     1021 tcacataacc atcgctcggc gcacgcacta tcgtctgctc aaggttatat tttgcttccg
     1081 cgagctgcgc tttcaggctg gcgatttgag aatgttcacc caacaccagg ctatccagct
     1141 ggctctggat ctgtttttgt tccgccgccg atgactttac ggaggcttcc tgcgccagat
     1201 aattttgccg cgccacatca atatcgcgtt ctgaaaacgg gtttactttc gcctggctgc
     1261 cgcgtgcgta acgctgatac tctttagcga atttatcccg cgtggccttt gcctgctgag
     1321 tattcgccgc catctcatct aactctgcgc ccaacgcccg ctgtttatgt tctgcggtaa
     1381 cgatatccgc catcagccga tccacccgcg cctgataacg cgtcgggtcc agtcgaaata
     1441 gcacctcacc ttttttaatc agcgtatttt tcttatccgt cacttcgatc accacgcctg
     1501 tcacctgtgg gacaacagga atagaaataa ccgctttttg cgccttgaag gtatacggat
     1561 ggttatagtt cattaacaga attaaaccac tgacaataaa tatgccaccc agggccgctg
     1621 tggaaatggt ccatttattt acaggaattt tgaagatctt aaacattgcc catgcaaaag
     1681 ccacataggt caaaataatc agtaaatcca taagtattac tcggcagaag aggatttgat
     1741 gtcggcgagc tgtttctcca gcccggcaat acgctgttgt agttgcacaa cggaggagtc
     1801 atggatttgc attccccatc cccgctccgg gcggtaaagc gtggcccaaa tccacaaaaa
     1861 tggccagata acatgcagcg taaaaagact cacccaacca gcaacatgaa tggcgtcggc
     1921 atgaggatga ttacgttttt tagcaatcag gtagggaata tcatgtaaaa taataatccc
     1981 atagaatatc accagaaata caaaaataag cactcccagt gcaaaatagt ccaggaacat
     2041 atttccctca aagaatataa aaaagaacaa ttaacgcata ttatgaaatg ccatgatgca
     2101 gtgcaatttc cttatttata taacgacata ttgtttttta tatactttct gaaatgtgct
     2161 gtctggtttt tcaacaggac gaggtttatc atattgaaat agtggaacta ttggccaaac
     2221 taatgaataa ctccagtaaa acatccacag tacagattaa gcgtattaaa ccttcaatta
     2281 tctaccgttt attgctgatt ggcctcggat caccaatggt gatttacggc ctggttcgcc
     2341 cgctcaccat cgaaacgcga gattaaactc ctgacaaaac acgccccaga aacgcacttc
     2401 tgttggcgta gtctgggtta ttgcgcacgt aggtttaaaa cgtaaattgt aacaacgtgc
     2461 gctttgttta tgccggatgc ggcgtgtacg ccttatccgg cctacggtca ggttcccgta
     2521 ggcatgataa gacgcgtaag cgtcgcatca ggcaatgaat acccaatgcg accagcttct
     2581 tatatcagaa cagccccaac ggtttatccg agtagctcac cagcaggcac ttggtttgct
     2641 ggtaatgctc cagcatcatc ttgtgggttt cgcgaccgat acctgattgt ttgtagccac
     2701 caaacgcagc atgtgccggg taagcgtgat aacagttggt ccacacgcgc ccagcctgta
     2761 tgccgcgccc catcttatag gccagattac cgttgcggct ccagacgccc gcgcccaggc
     2821 catattgcgt atcgttcgcc agctccagcg cttcttccat cgttttgaag gtggtcaccg
     2881 ccagcaccgg gccaaaaatc tcctcctgga acacccgcat attgttctga ccaaacagaa
     2941 tcgtcggttc gaggtagtag ccgtctttca gttcaccttc cagcagcttg cgccgcccgc
     3001 ctgtgagcac gtcagcgccc tcttttttac cgatatcaat gtagttgagg atggtttcca
     3061 gttgcccgtg agaaacctgc gcgcccattt gcgtcacgct gtcgagcggg ttaccgctgc
     3121 gaatgctttc gacacggcgg atggcgcgtt ccataaagcg ttcgtagatt gactcctgca
     3181 ctaaggcacg actcggacag gtgcaaactt cgccctggtt aaaggcaaac agtgcaaagc
     3241 cttccagcgc tttatcgaaa aaggcatctt cttcatccat cacatcagca aagaagatat
     3301 ttggcgactt accgcccaac tccagcgtca ccggaataat gttttgcgtt gcgtattgca
     3361 taatttgttg gcccacttcc gttgagccgg taaacgccac tttggcgatg cgtttcgagg
     3421 tcgccagata ttcgccaatt tccccacctg cgccattgac cacgttcacc acgcccggcg
     3481 gcagtaaatc accgacaatt tccattagca gcagtacaga aagcggggta agacgtgcgg
     3541 gtttcagcac cacacagttg cccgccgcca gcgcgggagc cattttccag ctcgccatca
     3601 gcagcgggaa gttccacggg ataatctgcc ccaccacgcc taacggttca tggaaatgat
     3661 aggccacggt ttcgctatca acttcactga tcccaccttc ctgcgcccga atacacgagg
     3721 cgaaatagcg gaaatggtca atcgccagcg gtacatccgc agcactggtt tcgcgaatgg
     3781 gtttgccgtt atcccaggtt tcagctgtcg ctaacagctc gaggttttgt tccattcgat
     3841 cggcaatctt aaacagaatc gccgcgcgat cctgcaccga ggtgtgcgcc catttatctt
     3901 tcactttgtg cgcagcatcc agcgccagat cgatgtctcg tttgcccgaa gacgccactt
     3961 cgcacagcag ctgcccggtc accggcgtca gattctggta atactcacca tcggcagggg
     4021 ctacccattc accgccaata aagttgtcgt agcgggcttt taacttgatg gggaaaccat
     4081 actcgccggg cttaatctgt gctgaagggg gattattggt catgatcgtc tccttgcggt
     4141 gtgaggtata acaagggtag acgtgactgg cgaaatcttc gccagtcggt aacagcttta
     4201 cgacagctat cacgaattta cgggcaagga ttacagagat agttgaaaaa gcggctgaca
     4261 atttgccagc cgttgttgga atgatgatta cattgcggcg cgataaatca ccacaatttc
     4321 ttcgtgagtt gcctggatcg ggttagtaaa gccacaggca tctttcaggg cattagtcgc
     4381 cagaaccgcg aaatcttctt ctttcacgtt caggtcgcgt aggcctgccg ggatatccac
     4441 tttcttcgcc agttcacgga tggcgttaat gcaggcttca gcaccttccg cgtcgttttt
     4501 acctgtcacg ttcacgccca ttgcagcggc acagtcacgc agacgtgcag cggcgacttt
     4561 gctgttgaat acctgaacat gcggcagcag aacggcgtta catacaccgt gtggcaggtt
     4621 gtagaaaccg cccagctggt gcgccatcgc atgaacataa cccagagaag cattattgaa
     4681 cgccataccg gcaaggaact gggcataagc cattgcttca cgcgctttcg cattactgcc
     4741 atcttcaacg gctaacggca ggttttcggc aatcatggtc acggctttca gtgcacaagc
     4801 gtcagtgatc ggcgtggcgg caatagaaac atacgcttcg atagcgtgcg ttaaggcatc
     4861 catacccgtt gcggcggtca gtgacttcgg cataccaatc atcagagagg agtcattgac
     4921 agaaagcagc ggagtcacgt gcttatcaac aatcgccatt ttgttatgac gcgcttcgtc
     4981 agtgatgatg cagaaacggg tcatttcaga ggccgtaccc gccgtggtat tgatggcgat
     5041 catcggcagc tgcggttttg cagagcggtc aacgccttcg taatcgcgaa tatcgccgcc
     5101 attggctgcc accagcgcaa taccttttgc gcagtcgtgt ggagaaccac cgcctaagga
     5161 gatcacgcta tcgcaattat tttctttaag taatttcaaa cctgcggcga cgttttccgt
     5221 ggtggggtta ggttgggtgc catcataaat aacgctaaaa atattgcgtt cttccagtgc
     5281 tttttgcaca tcacccgcca tacctaattt cgttaacata ttgtcagtga caattaaggt
     5341 acgggtaaat ccataatctg ccatcatatt cattgcatca gtcaatgaat cagcgccgat
     5401 gacattcaca gaaggaataa agaaagttga agatgccata acacactcct gaaagtgtaa
     5461 aaaagggtgg gcagcttact gaggattttc attcgaaaac atgatcatta ttattaatta
     5521 tcaaaattaa tctaataaaa agcacattgt attcattaaa caatgtgctt tatttaaaat
     5581 cataagaaat ttgtattaat ttattttaaa aattaattaa tcatttcctt atttttccgg
     5641 aaataataat gcgtcgcgca ataaatgatc atttccacga cgacgcgtaa agccaatgcg
     5701 gtcaaaatat tccagaatct gaattgccag ctttcggcct acgcctaagc gatcgcggaa
     5761 atccgccgcg caggttgaac cacactcctg atcgagatcg cggatcatat tggcaaattc
     5821 gacaatccga tcgttacggt aataacgatc tttgacgatc gcggtaatta ttccttgctg
     5881 cgccgcctgg cgtagcgtca ggcgcattgc ctgctcgtcg gtacccgtct cttttgccag
     5941 atcacgcacc caccacggct cgtcaccaaa cagcggctct gctttttgcc agatggcctg
     6001 ctgctcttcg ctgaagcccg ctttgtgatc cggcagatgc agccaaccgt gatgactgag
     6061 gatgtcgccg ctttcgcgca tcttttcaat cagcaacagt actagcgctt catcttccat
     6121 tggcaacgcc atacgtcgca gacgttcgcg cccaggccca ggttcatcgc gatgttgctc
     6181 atgataagtc gctaatgtgt cgagaatttt ccgttgccag cgagcggcaa ccggcgcatt
     6241 caacaagcta taaccagcct gaatatagcc aggctgttgc agcagttcgc gcatcccttc
     6301 gccgttgagc tggcgcgccc aggcgaaatc aggaagatta accgcgccgc gttgcaggtg
     6361 aacggctaac gcttcgctat cattctgtgc cctggcgagg gaagccagcc attgcagata
     6421 ctctggttta cgtttaccgc gacgcggcgg gttaagcatc acgacgcgcg ctccggccag
     6481 cgtgttgcgg gcagagatat cgcgcaatac caggcggtcg ttatctgcca gccataacgg
     6541 ggtgtcgaag accagttcag caaggttatc ttccagcagt gaaacgcgtc ccgtgacgtg
     6601 gctggcggcg tggtgaatat gcagcggctg ccactgggtc agcggtgcat gggtttgaag
     6661 ctcgacaatc acccgtgtga acggctctgg cggcacatcg gcaagcagcc agtcgccacg
     6721 gttaatctgc tctttttccg catcacccgc gatgtcaagc gcgatacgct gcccggcatt
     6781 ggcggtttct gttggctggt tttgcgcatg cagcgcacgt acacgcatcg gtttatttac
     6841 accagtcagc cagagtgaat cgcctacctt cacttccccg cttaacgccg taccggtgac
     6901 gaccagcccg gcacctttta cggtaaatgc gcggtcaatc gcgaggcgga aactatgttg
     6961 gctggcgtgc tcgcgttccg gcaactgaag cagatgctcg cgcagggcat caattccccg
     7021 accttcggtt gctgcggtga taaacagttt tgcctcagca aaaccgtatt cccgcagaac
     7081 ctcctttacc tggcgttcaa cctcatcaac acgcgcttcg tccacgcgat cggctttggt
     7141 cagcgccact gtcagcatcg ggttaccggt cagctgcaaa atcgccagat gctcacgggt
     7201 ctgtgccatc acgccgtcat cgcacgccac caccaacagc gcgtgatcga taccaccaac
     7261 gcccgccagc atgttggaaa gaaacttttc atgaccggga acgtcgataa aaccaggcac
     7321 gcgaccatcc ggctgcggcc agtaggcata gccgagatcg atggtcatgc cgcgcttttt
     7381 ttcttccggc agacggtcag catttacgcc agtaatcgcc tgcaataagg ttgttttgcc
     7441 gtggtcaacg tgtccggcag tcgcaataat catttcaaca acatctccaa aaaccgttgc
     7501 tcatcttcaa ggcagcgtaa atccagccac aatcgtccgt cataaatacg accaatcacc
     7561 ggcactggca attcacgcca gcgggcggct aatgactcaa ggtggctacc gcgtccatca
     7621 tggggtgtaa acgttaatgc cgcgctcggc aggcgatcaa ccggcagcga accactgcca
     7681 atctgcgaaa gacatggcat aacctgtacc gcaaactccg cgccgtaatg tgcgacaagg
     7741 ggagcctgta aacgttgtgc ctggatttga atgacctctg cgctgcgggt aagcaggcgc
     7801 agggtcggta atttttcact cagagcttca gggtgtaaat aaagacgcaa cgtggcttcc
     7861 agcgccgcga gggtcatttt atccgcgcgt aatgcacgct tcagcgggtg gctttgcagg
     7921 cgggcgatca tctctttttt accaacgata atcccggcct gcggcccgcc taacaacttg
     7981 tcgccggaga agctcaccag actgacgccc gccgcaatca actcctgcgg cattggctct
     8041 ttcggcaaac cgtactggct aagatcgacc agcgagccac tgcctaaatc agtcaccact
     8101 ggaacatcca gctctttgcc gagcgccacc agttcagctt cgtctatcgc tttggtgaac
     8161 ccctgaatgc tgtagttact ggtatgtact ttcatcaaca gtgcggtatt ttcattcacc
     8221 gcctgacgat aatcattcgc gtgcgtgcgg ttggtggtcc ctacttcgtg tagggtgcag
     8281 cctgcctgac gcataacatc gggaatacga aacgcgccgc caatctccac cagttcgccg
     8341 cgagatacca ccacctcttt cccgctggca gtggccgcca acatcaataa caccgccgcc
     8401 gcattgttat tgacgataca ggcatcttcc gcccccgtaa tacggcacag cagctgcgcc
     8461 agcgcccgat cgcgatgtcc gcgtccggcg tcgtccagat cgtactcgag ggtcactggc
     8521 gaacgcatag cctgcgcaac ggcttccacc gcggcttccg cctgtaaagc tcgcccaagg
     8581 ttggtatgca gcacggttcc cgtcaggttg atcaccggac gcagcgcgct ctgcgcctct
     8641 ttcgtcaacc gggcatcgac ttcttgcgcc cagttttcac accacgcagg cagcgtctgg
     8701 ctgccacgaa tcacttctcg cgcttcgtcg agcatctgac gcaacaattc caccacgcgg
     8761 gtgtgaccat aagtatcacg caaagaaagg aaggagctat cgcgcaataa gcgatcaata
     8821 gccggaagtt gactatagag ggaacgcgtt tcggttgtca taggaaacct ggctgatcaa
     8881 ggccctctca cacggagaag ggcgtttaac ataaccacgg attgtaacgt gagatgggtc
     8941 aggaggacat atcgcgcatc aagcctttgg cggttcggtg cgagcaaaac tttcgcggct
     9001 aaacaggttt tcgaccagtt tgactaagtg tgggcggtcg acacaccaac ctggcgcaac
     9061 gcggcggaaa ttgagatatc cgacagcaca ggcaatggcg atagtcgcca gattgaccgt
     9121 atcggttttg agtgtgccat cgaccagata tccttccagc acatccagac tgcggttgat
     9181 tttctcccgc tggcgtaaca attcatcttc agactgctgc gccgccggac gcgcctgttc
     9241 acgcaccgat accagcccgg catccataat gccatccgcc agcgcctcaa ttttgcgcac
     9301 ccgcaacgac tccagcggat cgcgcggcaa catcgccgga gcgacattca ttaactcaat
     9361 atattcagcg atgatcggcg aatcaaacca gcattcgccc tcttcggtca ccagcaccgg
     9421 cactttccct aacgggttaa attgcgccac gccgttatcc gcgttatagg gcagttcatt
     9481 aataaattcg aaagttatgc ccttttctaa caacagaata gaaagtttgc gtacaaacgg
     9541 gctggtgtag ctaccgacga gtttcatgcc gagtcctttg tgcgaggaaa aatatcagta
     9601 tggctcaggc tcacgcaaaa cacaggtgat ataagtctca atgatccaaa taaagataat
     9661 gcatccaact ggtcattctt agcagcactt tccgcatcac cgaaacatgg ctgaaatggt
     9721 cggagtaaac cgccacctgg gcgtagatgc cgtcgggtaa ggcatcgata tcatcgttag
     9781 ggtccagttc aatggttccc agcacaccgt ccgtgccggg aacgaccgtt aatgattgca
     9841 ataccccctg cgcctgataa gaaccgcctg gcacgacagg taaaatacta gtcaatttgc
     9901 cgtggaacac ctgcccaggt agcgcgttaa acaccacttc cgcatcatcg ccaggtttca
     9961 gacgtaacag cgagttttgc cgaaattggg cgacaatttg ccgtttttgc tcggggatga
    10021 agaccatcac cggacgcagc ggcaaggcag ctgcgtatgt acctgggcgg atcagtacct
    10081 gagtgacgta gccattgctt ggtgcgcgaa tgacagtctg ctcaagatta tattttgctt
    10141 cagtaagttg cgctcttaag ctcacaatct gcgattgctc gccgttgacc atactgtcga
    10201 gctgactctg gatctgcgcc tgctccgcca ccgagccttt cactaacgca tcctgcgcga
    10261 ggaaattttg ccgcgcatcg tcgatgtcac gttccgagaa cgaattcacc gccgcctggc
    10321 tgcctttcag gtaacgttga taatttttaa acagacggtc gcgctccgct gaaacctggg
    10381 tggtgttggc ctgcgcttca gtgagctgcg cacgcagcgt ctttatatta tgcgtcgccg
    10441 tcatcaggtc agcctgaagt ctgtcaactc gcgcctggta acgaaccggg tcgagcttaa
    10501 aaagcacctc gcccttttga ataagctgat tattcttgtc agtgacttca gtaacaattc
    10561 ccgtcacctg tggcgtgata gggatcgcta tcactgcctt ttgcgcggta aaagtgtaag
    10621 ggtggttgta gttcatcaac aaaatcaaac cactcaccag aaacacgcct cccagcgccg
    10681 ccgtcgccag cgccacgtaa gttaaaacaa tcaatagatc cataattaga tctccgggaa
    10741 cgtggaatag tcagtctttt tctcggcggc gagttgatgc tccagccggg aaattcggtc
    10801 agaaagtgcg gcgatttccg gttcagtcgc tttctcctgc gacgcaacat gtgactgcat
    10861 accccagcca cgctccggtt gatagagcgt cgcccagatc cacagaaacg gccagataac
    10921 atgcagagta aacaggctca cccagcccgc cgtatgaata gcgtcggcat gggggtgatt
    10981 gcgctttttg gcaatcagat aagggatgtc atgtatcgcg atgattccat aaaaaatcac
    11041 cagaaagacg aagatcagca ctcccaacgc gaaatagttt agaaacatat ctgcctcgga
    11101 ttcacgttta tcagtgttgt tttttggctg gcagccagaa gggagtcagg ctgatatttt
    11161 gacaataatc cgggttcgcg attctcgcca taacaccaaa gaataatttt tagaggtgat
    11221 gagttgctta gttacataac gattgtatga cgaaggcata acatgctgta gatcacatca
    11281 ggtgaacgcc gtaagaaaat atcttgtgat tcagatcaca aagattcaac aaaccatcaa
    11341 aacaaaaatg tgacattact cacatttaaa tgccattttt agcgaaaatc accgccttgt
    11401 ttctttttta tacaggcgtt ttgtgatgag cgtcacgtca attacctctc taccccctat
    11461 atttatgtga ctgatatcac acaaaagccc gtcgactgga cagttaaccg attcagtgcc
    11521 agatttcgca gtatctacaa ggtccggcta cctctgccgc cacattaaca aaaaacctcg
    11581 ggcttccagc ctgcgcgaca gcaaacataa gaaggggtgt ttttatgtca tccgatatta
    11641 agatcaaagt gcaaagcttt ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg
    11701 cgtttatcgc gtggggtatc atcaccgcgt tatttattcc aacagggtgg ttaccgaacg
    11761 agacgctggc gaagctggtc gggccgatga tcacttatct cctaccgctg ctgatcggtt
    11821 ataccggtgg taagctggta ggcggcaaac gtggtggcgt agtcggtgcc attaccacca
    11881 tgggcgttat cgtcggcgca gacatgccga tgttcctcgg ttctatgatt gcaggtccgc
    11941 tgggcggctg gtgcattaag cacttcgacc gctgggtaga cggtaagatc aaatccggtt
    12001 ttgagatgct ggtgaataac ttctccgcag gcatcatcgg gatgatcctc gctattctgg
    12061 cattcctcgg cattggcccg attgttgaag ccctgtccaa aatgctggct gcgggcgtta
    12121 acttcatggt tgtccatgac atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa
    12181 tcctgttcct caacaacgcc attaaccacg gtatcttctc gccgctgggt attcagcagt
    12241 cccatgaact gggtaaatca atcttcttcc tgattgaagc taacccaggt ccaggtatgg
    12301 gcgtgctgct ggcgtacatg ttctttggtc gtggtagcgc taaacagtct gcgggcggtg
    12361 cggcaatcat ccacttcctg ggtggtatcc acgaaatcta cttcccgtat gtgctgatga
    12421 atccgcgtct gatcctcgca gtcatcctcg gcggtatgac tggcgtgttc acgctgacta
    12481 tcctgggcgg tggtctggtt tctccggcat ctccgggttc tatccttgct gtactggcga
    12541 tgacaccaaa aggtgcttat ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct
    12601 ccttcgttgt ctctgctatt ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg
    12661 aagcagcaac tcgtcgtatg caggacatga aagctgagtc taaaggcaca tctccgctat
    12721 ctgcgggcga tgtgactaac gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg
    12781 ccggtatggg ttcaagtgcg atgggcgcag gcgtgctgcg taagaaaatt caggatgcag
    12841 gtctgtcgca gatttcggtc actaacagcg cgatcaacaa cctgccgcca gatgtggacc
    12901 tcgtcatcac tcaccgtgac ctgaccgaac gcgctatgcg ccaggttccg caggcacagc
    12961 atatttcgct gaccaacttc ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg
    13021 ttgctgccca gcgccatact gaaaacgaag tgaaagtgaa agacagtctg aaagacagct
    13081 ttgacgattc cagtgctaac ctgttcaaac tgggcgcgga gaacatcttc ctcggtcgca
    13141 aagcagcaac caaagaagaa gcgattcgtt ttgctggcga gcagctggtg aaaggcggtt
    13201 acgttgagcc ggaatacgtt caggcgatgc tggatcgtga aaaactgacc ccgacttatc
    13261 tgggtgagtc tatcgcggtg ccacacggta cggttgaagc gaaagatcgc gtactgaaaa
    13321 cgggcgtggt gttctgccag tacccggaag gcgtgcgctt cggtgaagaa gaagatgaca
    13381 ttgcccgtct ggtgattggt attgcagccc gtaacaacga gcacattcag gttatcacca
    13441 gcctgaccaa tgcactggat gatgagtccg tcatcgagcg tctggcacac accaccagcg
    13501 tggatgaagt gctggaactg ctggcaggtc gtaagtaatc caatcctacc ctctcctcac
    13561 ggagagggtt tgggtgaggg aaaagcctca ccccagccct ctcgggtaaa aacattgatg
    13621 aaggttaata ctatgaaagc attacatttt ggcgcaggta atatcggtcg tggctttatc
    13681 ggtaaactgc tggcagacgc gggtatccaa ctgacgtttg ccgatgtcaa tcaggtggta
    13741 cttgatgccc tgaatgcccg tcatagctat caggttcatg tggtcggtga aaccgagcag
    13801 gtagataccg tttccggcgt caatgctgtc agcagcattg gtgatgatgt cgttgatctg
    13861 attgctcagg tcgatttagt cactaccgcc gttggcccgg ttgtgctgga acgtattgct
    13921 ccggctatcg ccaaagggct ggtgaaacgc aaagaacaag gtaatgaatc cccgctgaac
    13981 atcatcgcct gtgaaaacat ggtacgcggc accacgcagc tgaaaggtca tgtgatgaac
    14041 gccctgccgg aagacgccaa agcgtgggta gaagagcacg ttggctttgt cgattccgcc
    14101 gttgaccgca tcgtaccgcc ttcggcttcg gcaactaacg atccgctgga agtgacggta
    14161 gaaactttca gcgaatggat tgtcgataaa acgcagttca aaggcgcact gccgaacatc
    14221 ccaggcatgg agttaaccga caacctgatg gcatttgtcg aacgtaaact cttcaccctg
    14281 aacacgggtc atgctataac cgcgtacctc ggaaaactgg ccggtcatca gaccattcgt
    14341 gacgctattc tcgacgagaa aatccgcgcg gtggtaaaag gtgcgatgga agaaagtggt
    14401 gcggtactga tcaagcgcta cggctttgac gcagacaaac atgcggcgta catccagaaa
    14461 atcctcggtc gttttgagaa cccgtatctg aaagatgatg tagagcgcgt aggccgtcag
    14521 ccgctgcgta aactgagtgc tggcgaccgt ctgatcaagc cactgctcgg tacgctggaa
    14581 tacagccttc cgcacaagaa tctgattcag gggattgctg gtgcaatgca cttccgcagt
    14641 gaagatgatc cgcaggctca ggaactggca gcactgatcg ctgacaaagg tccgcaggcg
    14701 gcgctggcac agatttccgg tcttgatgcc aacagcgagg ttgtatccga ggcggtaacc
    14761 gcttataaag caatgcaata atggtggacc aggcgcagga caccctgcgc ccgaataaca
    14821 gattgtcaga tatgcaggca acaatggaac aaacccaggc cgttgaaaac cgtgtgcttg
    14881 agcgtctgaa tgctggcaaa accgtgcgaa gctttctgat caccgccgtc gagcttttga
    14941 ccgaggcggt aaatcttctg gtgcttcagg tattccgcaa agacgattac gcggtgaagt
    15001 atgctgtaga accgttactc gacggcgacg gtccgctggg cgatctttct gtgcgtttaa
    15061 aactcattta cgggttgggc gtcattaacc gccaggaata cgaagatgcg gaactgctga
    15121 tggcattgcg tgaagagcta aatcacgacg gcaacgagta cgcctttacc gacgacgaaa
    15181 tccttggacc ctttggtgaa ctgcactgcg tggcggcgtt accaccgccg ccacagtttg
    15241 aaccagcaga ctccagtttg tatgcaatgc aaattcagcg ctatcaacag gctgtgcgat
    15301 caacaatggt cctttcactg actgagctga tttccaaaat cagcttaaaa aaagcctttc
    15361 aaaagtaagc aacgtctgct tactgcccct ctacctgctt cggccgataa agccgacgat
    15421 aatactccag acgttgtaga tataacggca cgctttcatc gggtattccg gacggaatcg
    15481 cgttacgggg aggaagtttt ttcagatact cccggaacgc ctggcttgat gccatgaaat
    15541 ctacggcttt atcgataaga agcggaacgt tttcacctaa tttgcccatg atgatatctc
    15601 cgtattaccc cgcgccggga atgcgcggcc tccaatttta gtttaggttc ccggtaaaga
    15661 tgacattaag gaaaacgtgc tgaatcctca ataatcatcc gctgctgttt attatctttt
    15721 tctatagatt tcttaattaa tcaacgaatt gcgtagtttt tgagcatgtt aatgattacg
    15781 ttgcagaact attacaatgt catcagttgt gaacaaagca cctggtcgcg catactaggg
    15841 gctataaatt tatctttatc agaagccatc acatgaaaga agtcgaaaaa aacgaaatca
    15901 agcgtctcag cgatcgcctg gacgccatcc gccaccagca ggccgatctg tcgctggttg
    15961 aagccgcaga caaatatgcc gagctggaaa aagagaaagc cacgctggaa gcagaaattg
    16021 ctcgcctgcg tgaggttcat agccaaaaac tgagtaaaga agcacagaag ctgatgaaga
    16081 tgccgttcca gcgcgcaatt accaaaaaag agcaggctga tatgggcaag ctgaagaaaa
    16141 gtgttcgcgg actggtggtt gtgcacccaa tgaccgcgct ggggcgcgaa atgggcctgg
    16201 aagagatgac tggattttct aagactacgt tttaagcccc tcagacgact ggttcgatcg
    16261 tatcaacccg catctggctt gccttagctg gtcaatgcgg ggactctcct gtctactcgc
    16321 ctaattacca aacaattcac tccctttact tcgatttacc gctggttgat attctgaaaa
    16381 tgcattttta ccgaaatagc catctataaa caataaaaat atatagaaga tatcatttgt
    16441 taattaacaa gcatttacgt ctcttttttt cggatttaat tgtgaaattt taatcatcat
    16501 attcacaaaa tcaattatct gtgaaatttt tcccagacat ggtttctttc atatttgaca
    16561 tttattaata atcatcatta gaatccacga aaaataatat attcatacaa acacagacat
    16621 cttttgaaac gaatgataca tttttatgat gaatgcttaa aacaatattt aatcgacaca
    16681 catcactgtc attaatgtaa agcaacatag ctcaccaata atacaatgga caactctacc
    16741 ggcattgatc gctggtcgga ggaaataagg atttttacat gaacttgaag aagatttttt
    16801 tcagtgctgt cactgtttca gttctctgcg cattaacggg atgtgactac attgaagaag
    16861 gcaagccaga aagtagttta ctgaaacaac aagaagaaca taataataaa attgtgctac
    16921 ttgaaaaaca acaagcacag ctaaagagtc aactcgaaac tattcaaaaa caacaaactg
    16981 gcataataaa cagtacaaag actttaactc acgtaattaa atccgttaaa gaccaacaaa
    17041 atacttttat ttttaccgag tttaacccag caaaaacgaa gtatttcatc ctgaacaacg
    17101 gttcggtggc tttagcgggt cgagtgttat ccattgacgc cacagagaat ggtagtgtta
    17161 ttcatatctc actggtcaac ttattaagta cacctatctc aaacattggt tttaatgcga
    17221 catggggtgg tgaaaaacct gtcgatgcca aagagtttgc ccggtggcaa caattacttt
    17281 tcaacacatc aatgaaatcc acattgaaat tattaccagg tcaatggcaa gacattaatt
    17341 tgaccctgaa gggtgtatcg cccaataacc tgggatatct gaagttagcc atcaacatgg
    17401 aaaatattca gttcgacaat cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaat
    17461 aaatgcagtt agtacgctta taacaaggaa tagcgagcat cattaatgaa caaaatattt
    17521 aaagttatct ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc
    17581 cgtggcaaga aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg
    17641 ttgtcgtcgt ttggggcatt ggcgaatgcc gggaatgaca acggtcaggg tgttgattac
    17701 ggtagtggat cagctggcga cggctgggtt gctataggca aaggggcgaa agcaaatact
    17761 tttatgaaca ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa
    17821 tatagctctg ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg
    17881 gttagtgcaa tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg
    17941 ggccaatact caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct
    18001 atgggggact cttccaaagc ggaaggagca aacgccattg ccctggggcg tagcagtgta
    18061 gctagcggta ctgacagcct cgcatttggc agacaatcac ttgccagcgc agcgaacgct
    18121 attgcgatag gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg caataatgcg
    18181 aaggcaaaag ggactaatag catggcaatg gggttcggaa gccttgccga taaagtcaat
    18241 actatcgcat taggaaatag cagccaggct ctggcagata atgcaatcgc cataggccag
    18301 ggcaacaaag ctgatggcgt ggatgccatc gctctgggta atggtagcca gtcgagaggc
    18361 ttaaacacca ttgccttagg cacagccagt aatgcaactg gtgataagag tcttgcgctt
    18421 ggtagtaata gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga ttccattgcg
    18481 gatttagaca ataccgtctc tgtcggcaat agttcattaa aacgcaagat cgttaatgtg
    18541 aaaaatggcg cgatcaagtc tgacagttac gatgccatta atggttcaca gctttatgcc
    18601 attagcgact cggtagcaaa aaaggcttgg aggaggggct gcagtagatg ttgatgacgg
    18661 tactgttaca gcaccaacct acaatttaaa aaatggtagc aaaaataacg taggggctgc
    18721 gctcgctgta cttgatgaaa acaccctgca atgggaccaa accaaaggca aatacagcgc
    18781 tgctcatggt actagtagcc caactgccag cgtaatcacc gatgttgcgg atggcacgat
    18841 ttcagcctcc agtaaggatg cggttaacgg ttcccaactg aaagctacca atgacgatgt
    18901 cgaagccaac accaccaata tcaccaacct gacggattcc gttggtgacc ttcaggctga
    18961 tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc aggataccac
    19021 cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg atgctgttaa
    19081 cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca atatcgccaa
    19141 taacacttcc aatattgcca ctaacaccac caacatctct aacctgactg agacggtgac
    19201 taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca cggcagctca
    19261 tggcaccgag accaccagca aaataaccaa cgttaaagat ggcgacctga cgactggcag
    19321 caccgatgcc gttaacggct ctcagctgaa aaccaccaac gatgccgtgg cgacgaatac
    19381 caccaatatc gccactaaca ccaccaacat ctctaatctg actgagacgg tgactaatct
    19441 tggtgaggat gcgctgaaat gggataagga caatggtgtc ttcactgcag ctcatggcaa
    19501 caataccgcc agcaaaatca ccaatatcct ggacggcaca gtcactgcaa ccagttccga
    19561 tgccattaac ggtagccagc tttatgactt aagcagcaat atcgccacct acttcggcgg
    19621 caatgcttct gtgaatactg acggtgtgtt taccggtcca acctacaaaa tcggtgaaac
    19681 aaattattat aacgtcggcg atgcactggc tgcgattaac tcctcattta gcacgtctct
    19741 cggcgatgct ctgctttggg atgccaccgc aggtaaattc agtgccaaac acggtactaa
    19801 tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa atttcagact ccagttctga
    19861 cgcagtaaac ggctcacaac tccacggcgt gagcagttat gttgttgatg cgctgggggg
    19921 tggtgccgaa gtcaatgcag acggcaccat caccgcaccg acttacacca ttgcgaacgc
    19981 cgagtacaac aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga
    20041 taagactgcc aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat
    20101 catcactaat gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc
    20161 tcagttgaat gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc
    20221 tggtaacacc gacgcaacct atatcgaaga aaatggcgct ggtatcaact acgttcgtac
    20281 taacgacaac gatttagcct ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt
    20341 aggttataac gctgtcgcgt ctggtgccag cagcgtagcc attggtcaga acagcagcag
    20401 caccgttgat accggtattg cgctgggtag cagctccgtt tccagccgtg tgattgccaa
    20461 aggttctcgt gacactagcg taacggaaaa tggcgtggtg attggttatg acaccactga
    20521 cggcgaactg ctaggtgcat tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa
    20581 cgtagctgat ggttcagaag cccatgacgc cgttacggtt cgccagttgc agaacgctat
    20641 tggagcggtc gccactacgc caaccaagta ctttcacgcc aactcaacgg cagaagactc
    20701 actggccgtt ggtgaagact cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc
    20761 aggtattggt atcggtttga acactctggt tctggctgat gcgatcaacg gcattgctat
    20821 cggcagtaac gcacgcgcaa accatgcaaa cagtatcgca atgggtaacg gttctcagac
    20881 cacccgtggt gcacagactg gctacaccgc ctacaacatg gacgcaccgc agaactctgt
    20941 aggtgagttt tctgtcggta gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg
    21001 ttcagcggat accgatgcgg ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc
    21061 gcaaaatacc cagagcatta ctaacctgaa caatcaggtc actaatctgg atactcgcgt
    21121 tactaatatc gaaaacggta ttggcgacat tgtcaccacc ggtagcacca agtacttcaa
    21181 gaccaacacc gatggcgtag atgccaacgc ccagggtaaa gatagcgttg ctattggttc
    21241 tggttccatt gctgccgctg acaacagcgt cgcactgggt accggttccg ttgcagagga
    21301 agaaaataca atctctgtag gttcttctac taaccaacgc cgtatcacca acgtagctgc
    21361 cagtgttaat gccaccgatg cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg
    21421 agtgcgctac gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg
    21481 tggcaacggc agtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc
    21541 ggtgaactac gcgcagttga agcaaagcgc gcaggaaacg aagcaataca ccgatcagcg
    21601 gatggttgag atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc
    21661 ttctgcaatg gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc
    21721 tattggtggc ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag
    21781 cgccaatggt cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc
    21841 cgccgcactc ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc
    21901 aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac
    21961 aatctaaggg caattctctg atgaggattg cccttttctt taccagacat ctccccccac
    22021 aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa
    22081 aactcacatc aatattgcca atacataaca tttagttaac cattcattgt cattatccct
    22141 acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga
    22201 ctcattacac gatgtgcgtg gactctagga gacctgcaat gaatctctgg caacaaaact
    22261 acgatcccgc cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt
    22321 tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag
    22381 caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct
    22441 cagtggttta tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag
    22501 tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc
    22561 tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt
    22621 tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg
    22681 gcctgggttt taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag
    22741 tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca
    22801 gctttgagat tggccagatg gtggggcgtc agctaccgtt tatgaccatt atcgtgctgt
    22861 tctggatcat ggcgattatg gacggctggc gcggtatcaa agagacgtgg cctgcggtcg
    22921 tggttgcagg cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg
    22981 agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac
    23041 gctggcaacc agttcgcgta ttccgcttcg gtgacttggg ggcgtcacag gttgatatga
    23101 cgctggccca caccggttac accgcgggtc aggtgttacg tgcctggaca ccgttcctgt
    23161 tcctgaccgc taccgtaaca ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg
    23221 gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc gtacctcaat aaactggttg
    23281 cccgtatgcc accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact
    23341 ggttctctgc caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga
    23401 agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctac
    23461 ccatctactc catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggactgt
    23521 catcaacact ggcactggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt
    23581 tcctcggctg gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg
    23641 ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg
    23701 ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct
    23761 gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca
    23821 gcctgatctt cacctgtatg gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt
    23881 ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt
    23941 gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca
    24001 actggcgatg caactcggcg tatcacgtaa ttcactacgc gaggcgctgg caaaactggt
    24061 gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga
    24121 cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg atgatccgga
    24181 ttacagtttc gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc
    24241 ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct
    24301 aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga
    24361 agcctcacat aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc
    24421 ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac
    24481 cgaacaacat caggctgtca ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa
    24541 agcaatgatg gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca
    24601 ggctcgccac gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa
    24661 aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg
    24721 ccgttcctgt tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac
    24781 gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat gtccgactta
    24841 agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg
    24901 ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg
    24961 catggcattc cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca
    25021 gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt
    25081 aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg
    25141 ccgacaccgg gcgcacgcta ccgtgatgcg cattcaggta tgagcggccc aaatgcggca
    25201 atgcgccgct acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac
    25261 ggtcgtccac atgatttagg taatatctca gcttatctcg gcaaaccgac cggactggaa
    25321 gattacatcg gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag
    25381 tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat
    25441 gcgcgcgatg cagtacgttt tggtgctgat ggaattgtgg tttctaacca cggtggccgc
    25501 cagctggacg gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa
    25561 ggtgatatcg ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg
    25621 attgcgctcg gtgccgacac catactgctg ggtcgtgctt tcctgtatgc gctggcaaca
    25681 gcgggccagg cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg
    25741 atgacgctga ctggcgcgaa atcgatcagt gaaattacgc aagattcgct ggtgcagggg
    25801 ctgggtaaag agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg
    25861 tttgcgcccc ctcaccctaa ccctctccct cagggagagg ggaccgttcg gcgctgtatg
    25921 tactccctca ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggagg
    25981 gccggggtga ggggaaaagg ccgcacggtt caccaatttt ctgctatcct gcccccgcac
    26041 taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc
    26101 aacatcatcc gtctttgcgc taataccggc tttcgtctac atatcatcga accgatggga
    26161 tttgcctggg acgacaagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc
    26221 gttacgcgtc atcatgacta tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt
    26281 gccctcacca cgaaaggtac gcctgctcac agcgccgtaa gctatcagga tggcgattat
    26341 ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct
    26401 gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaatct gtccaatgcg
    26461 gtgtcggtag tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga
    26521 gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt
    26581 agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca
    26641 ttgatggctt atcgctgtct ggtttaccga caatacgaac cggaacgcca gcggcggttg
    26701 tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa
    26761 cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg
    26821 ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata
    26881 cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa
    26941 tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt
    27001 tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat
    27061 aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg
    27121 ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt
    27181 cagcggcgta ggcttcttca accacttcac ggatagcaat agcaggcata attggcgatg
    27241 acagcttgtt cgccagcatg tagctcagtg cactgccaag gttttcgtgc ttgagtagcg
    27301 tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt
    27361 taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag
    27421 ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt
    27481 tagtgactgc tgcgctcgtc cttgcgtgca cgacctaata atgtcaatgc tgcctcgcgc
    27541 gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat ttcaacgccg
    27601 aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca
    27661 atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatacc aaaacggcgg
    27721 ttacgcgact ggttgtcggt acaggtaagc accagatcgc ccagccccgc catgcccata
    27781 aaggtggcag gatcggcacc cagcgccgca ccaagacgcg acatttcagc cagcccacgg
    27841 gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac cgtcagacat ccccgcgcca
    27901 atggcaatga cgtttttcac cgcgccgcca agctgcacgc caatgaaatc cgggttgctg
    27961 taaacgcgga aacttttgcc gcagtgcagc agctgctgga gatcatcggc aaaggtctga
    28021 tcggtcgagg ccagcgaaat agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt
    28081 gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt
    28141 aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga
    28201 cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg
    28261 agaatattac gactggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg
    28321 ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata
    28381 tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag ggtaatggca
    28441 agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc
    28501 aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca
    28561 gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc
    28621 tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat
    28681 gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac
    28741 acaggaacgc ggtttcttcg cccaaggagg ccgttacggt aacacgcagt accacttcgt
    28801 atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc
    28861 aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa
    28921 tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct
    28981 taagtgttgt ccttaaatac tgcgcaatgt aataccaacg ttcacacgtt atttcagcag
    29041 gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg
    29101 tgcgtcaata aaaatctggg gaaccgtggt gcaaccgctg cgtttgatca tctcttcacg
    29161 cttggcggca ttgccatcga tcggcaactc ctggaaactc acgcccttgc tgctcagcag
    29221 tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat
    29281 aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag
    29341 tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca
    29401 tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt cgccagccca
    29461 gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt
    29521 tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccgatt tgtctttgtg
    29581 cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc
    29641 tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc
    29701 tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct
    29761 cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg
    29821 atgacggcca acaaattgca taatttcttg catggggggt aacaactccc gacgtagtga
    29881 ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa
    29941 ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc
    30001 gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat
    30061 tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat
    30121 ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt
    30181 tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga
    30241 cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca
    30301 cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat
    30361 caaagatcgc gctttctttg ctaatccagt gctggctggc gcggtacata aagcgaaaaa
    30421 cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga
    30481 agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct
    30541 gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa
    30601 atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta
    30661 ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac
    30721 tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc
    30781 tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga
    30841 tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg
    30901 tgaaatcact cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt
    30961 taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta
    31021 cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca
    31081 gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt
    31141 agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta
    31201 cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa
    31261 gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac
    31321 cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt
    31381 cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc
    31441 tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt
    31501 tccgctgatt tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga
    31561 catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa
    31621 gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac
    31681 acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag
    31741 cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc
    31801 tattcaggcc gatatcgccg caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc
    31861 aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct
    31921 gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc
    31981 gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga
    32041 tgccgcattc cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag
    32101 ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac
    32161 cattgcccag ttgaagcaaa cgcgtgaaga agtcaccatg cagcgtgctg aactggaaga
    32221 gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac
    32281 tcaggctctg aacgagcgta aaaagacgct ggcggggctg gaatcttcca tccagcaagg
    32341 tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg
    32401 tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg
    32461 cgagcgccag aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa
    32521 atcgctgatg tcccgaactg gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt
    32581 tcgcgggccg acgctgcatc gctatggcga gcagctacag ggtgaattac gctggaaagg
    32641 gatggttatc ggtgcctctg aaggtactga agttaaagca attgccgacg gtcgagtgat
    32701 tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga
    32761 catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc
    32821 gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt
    32881 cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt
    32941 gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt
    33001 acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa
    33061 ccaggtgctg gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt caccgcacgc
    33121 cagagagatg gcgaccaaag cgcataacag cggtcacgaa gtgttgattc atctcccgat
    33181 ggcaccgttg agtaaacagc cgctggagaa aaatacgcta cgcccggaga tgagcagcga
    33241 cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa
    33301 caaccacatg ggcagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc
    33361 gctggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat
    33421 gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc
    33481 gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa
    33541 cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat
    33601 ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc
    33661 gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt
    33721 ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt
    33781 ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca
    33841 gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt aacgctagcg caaattaaag
    33901 gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa
    33961 gaccacagta acgcctggta accgaccttc acgctgcgca cattggtaat caggcgctta
    34021 tacatacccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc
    34081 tttgcgcacc
//
LOCUS       DAEEGA010000039        33686 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEEGA010000039 DAEEGA010000000
VERSION     DAEEGA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33686
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.039"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            529..666
                     /locus_tag="KLH46_002007"
     CDS             529..666
                     /locus_tag="KLH46_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378698.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1266..2045
                     /gene="ipaJ"
                     /locus_tag="KLH46_002008"
     CDS             1266..2045
                     /gene="ipaJ"
                     /locus_tag="KLH46_002008"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HBE0378699.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2992..3921)
                     /gene="virB"
                     /locus_tag="KLH46_002009"
     CDS             complement(2992..3921)
                     /gene="virB"
                     /locus_tag="KLH46_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HBE0378700.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4138..4374)
                     /locus_tag="KLH46_002010"
     CDS             complement(4138..4374)
                     /locus_tag="KLH46_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBE0378701.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4378..6279)
                     /gene="ipaA"
                     /locus_tag="KLH46_002011"
     CDS             complement(4378..6279)
                     /gene="ipaA"
                     /locus_tag="KLH46_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HBE0378702.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6288..7286)
                     /gene="ipaD"
                     /locus_tag="KLH46_002012"
     CDS             complement(6288..7286)
                     /gene="ipaD"
                     /locus_tag="KLH46_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HBE0378703.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLNILSRN
                     GYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLENLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7337..8428)
                     /gene="ipaC"
                     /locus_tag="KLH46_002013"
     CDS             complement(7337..8428)
                     /gene="ipaC"
                     /locus_tag="KLH46_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HBE0378704.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(8448..10190)
                     /gene="ipaB"
                     /locus_tag="KLH46_002014"
     CDS             complement(8448..10190)
                     /gene="ipaB"
                     /locus_tag="KLH46_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HBE0378705.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10196..10663)
                     /gene="ipgC"
                     /locus_tag="KLH46_002015"
     CDS             complement(10196..10663)
                     /gene="ipgC"
                     /locus_tag="KLH46_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HBE0378706.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10720..11346)
                     /gene="ipgB1"
                     /locus_tag="KLH46_002016"
     CDS             complement(10720..11346)
                     /gene="ipgB1"
                     /locus_tag="KLH46_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HBE0378707.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="KLH46_002017"
     CDS             complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="KLH46_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HBE0378708.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="KLH46_002018"
     CDS             complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="KLH46_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HBE0378709.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13562..15178
                     /locus_tag="KLH46_002019"
     CDS             13562..15178
                     /locus_tag="KLH46_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HBE0378710.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15188..15550
                     /gene="ipgE"
                     /locus_tag="KLH46_002020"
     CDS             15188..15550
                     /gene="ipgE"
                     /locus_tag="KLH46_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HBE0378711.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15550..16008
                     /gene="ipgF"
                     /locus_tag="KLH46_002021"
     CDS             15550..16008
                     /gene="ipgF"
                     /locus_tag="KLH46_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HBE0378712.1"
                     /translation="MSRFVFILLCFTPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16017..17132
                     /gene="mxiG"
                     /locus_tag="KLH46_002022"
     CDS             16017..17132
                     /gene="mxiG"
                     /locus_tag="KLH46_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HBE0378713.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17140..17391
                     /gene="mxiH"
                     /locus_tag="KLH46_002023"
     CDS             17140..17391
                     /gene="mxiH"
                     /locus_tag="KLH46_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HBE0378714.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17404..17697
                     /gene="mxiI"
                     /locus_tag="KLH46_002024"
     CDS             17404..17697
                     /gene="mxiI"
                     /locus_tag="KLH46_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HBE0378715.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17703..18428
                     /gene="mxiJ"
                     /locus_tag="KLH46_002025"
     CDS             17703..18428
                     /gene="mxiJ"
                     /locus_tag="KLH46_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HBE0378716.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18425..18952
                     /locus_tag="KLH46_002026"
     CDS             18425..18952
                     /locus_tag="KLH46_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HBE0378717.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18894..19589
                     /gene="mxiN"
                     /locus_tag="KLH46_002027"
     CDS             18894..19589
                     /gene="mxiN"
                     /locus_tag="KLH46_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HBE0378718.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19582..19989
                     /gene="mxiM"
                     /locus_tag="KLH46_002028"
     CDS             19582..19989
                     /gene="mxiM"
                     /locus_tag="KLH46_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HBE0378719.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19973..20401
                     /gene="mxiM"
                     /locus_tag="KLH46_002029"
     CDS             19973..20401
                     /gene="mxiM"
                     /locus_tag="KLH46_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HBE0378720.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20531..21163
                     /gene="mxiE"
                     /locus_tag="KLH46_002030"
     CDS             20531..21163
                     /gene="mxiE"
                     /locus_tag="KLH46_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HBE0378721.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21150..22850
                     /gene="mxiD"
                     /locus_tag="KLH46_002031"
     CDS             21150..22850
                     /gene="mxiD"
                     /locus_tag="KLH46_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HBE0378722.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22869..23936
                     /gene="mxiC"
                     /locus_tag="KLH46_002032"
     CDS             22869..23936
                     /gene="mxiC"
                     /locus_tag="KLH46_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HBE0378723.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23949..26009
                     /gene="mxiA"
                     /locus_tag="KLH46_002033"
                     /gene_synonym="virH"
     CDS             23949..26009
                     /gene="mxiA"
                     /locus_tag="KLH46_002033"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HBE0378724.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26022..26423
                     /gene="spa15"
                     /locus_tag="KLH46_002034"
                     /gene_synonym="spaK"
     CDS             26022..26423
                     /gene="spa15"
                     /locus_tag="KLH46_002034"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HBE0378725.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            26427..27719
                     /gene="spaL"
                     /locus_tag="KLH46_002035"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26427..27719
                     /gene="spaL"
                     /locus_tag="KLH46_002035"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HBE0378726.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27813..28169
                     /gene="spa13"
                     /locus_tag="KLH46_002036"
                     /gene_synonym="spaM"
     CDS             27813..28169
                     /gene="spa13"
                     /locus_tag="KLH46_002036"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HBE0378727.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIRQYKPKF"
     gene            28138..29016
                     /gene="spa32"
                     /locus_tag="KLH46_002037"
     CDS             28138..29016
                     /gene="spa32"
                     /locus_tag="KLH46_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HBE0378728.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGLFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29010..29891
                     /gene="spa33"
                     /locus_tag="KLH46_002038"
                     /gene_synonym="spaO"
     CDS             29010..29891
                     /gene="spa33"
                     /locus_tag="KLH46_002038"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HBE0378729.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29896..30546
                     /gene="spa24"
                     /locus_tag="KLH46_002039"
                     /gene_synonym="spaP"
     CDS             29896..30546
                     /gene="spa24"
                     /locus_tag="KLH46_002039"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HBE0378730.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30561..30821
                     /gene="spaQ"
                     /locus_tag="KLH46_002040"
     CDS             30561..30821
                     /gene="spaQ"
                     /locus_tag="KLH46_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HBE0378731.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30821..31591
                     /gene="spa29"
                     /locus_tag="KLH46_002041"
                     /gene_synonym="spaR"
     CDS             30821..31591
                     /gene="spa29"
                     /locus_tag="KLH46_002041"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HBE0378732.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31598..32626
                     /gene="spa40"
                     /locus_tag="KLH46_002042"
                     /gene_synonym="spaS"
     CDS             31598..32626
                     /gene="spa40"
                     /locus_tag="KLH46_002042"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HBE0378733.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32639..32881
                     /locus_tag="KLH46_002043"
     CDS             32639..32881
                     /locus_tag="KLH46_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378734.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            complement(33488..33686)
                     /locus_tag="KLH46_002044"
                     /pseudo
     CDS             complement(33488..33686)
                     /locus_tag="KLH46_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT        10496 a         4914 c         6753 g        11523 t
ORIGIN      
        1 ttgttgacta cctgtgcggc atctggtatc aaggtggcgt gaagccggtt caggttctca
       61 tgcggaacgt tggaacctat atccccccga taccaaaggc gaaatgtaag tgacaaaatg
      121 caatgaacac gttctccgtt aactgaggag catggtgata aataaaggta aatgcagaag
      181 gtgtagagtc tcgcagattt ctgacatcat agagcctaac attagatgca taacgctaca
      241 gattgtagaa tctctacagg tgaaatatac actggttggc gtttatgtcg caaaacatgt
      301 cattcaggtt catctcacca ataaggatat gagtgaagtg gaggataagt gagtctgctg
      361 tcagagtttt ctggtgtatg tcagtaactg cgaaccatgt ctgcttgata tgggagctta
      421 tggaggctct ctgtacaagg ttagggagct aacaaagcag ggataaaaag tccgcctgat
      481 gcaggcacgg ttcgttaaat cttttgccat tggtaacaaa atggtgcgat gaatgttcag
      541 gctatcttta tcttgatggt ggttcagctg tttggtaaag aaatcgctgt aacaatagaa
      601 gaactgcagg cagtactggt cccacatcgt ttgctcagtc aactggtgaa gttccgtagc
      661 gcgtaaatta atgctttgca tgaaacgtta ctggagtgga tgaaagaata cctgcatata
      721 tcattactgc gttggattac cagtacaaca gattgaatga actggacatg ttgatagacg
      781 gcattgaaaa gcagcttata agcatagtgc ggcagagtta attggagagc acttcgcctt
      841 ctgagtatta agtaagcatg gtggtacctg cggatactat ctattcactg tgctcggtcg
      901 gcagccttac tgaccaagga gttgggggag ttgataacaa agttgaaaaa acagctctca
      961 accaaggtgg ttattgtaac aatagcaaat aagctggtgt gacctgtatc acaataaccg
     1021 attatggacg taaatacgac aaaaatatgt cagtatcagg ctgtattaat tgctgatgct
     1081 gatggacatt aactcttaat aaaagggtgg atgctgaaag gttgttatgg tgacggacgt
     1141 gatgacgacg gaaaagagta tgaggttttg gatttaaagt tcgttcgggg aataaggacc
     1201 cggtcagcta attctcatag tagttcgcat tgctggatgc aatactgtgc tgcaattact
     1261 cgtcgatgtc ggaacaacgg aagccttgca aacggggatg tattcataca ggtgttatgt
     1321 tatatggggt tttattgcag ggggctatac caagagaata tatgatatct catcagactg
     1381 atgtgagggt aaatgagaat agagtaaacg aacaaggttg ttttttggcg aggaagcaga
     1441 tgtatgacaa ttcttgtgga gctgccagtt tactctgtgc tgcaaaggaa ttaggggttg
     1501 ataaaatacc acaatacaaa gggtctatgt ccgaaatgac aaggaagagt tctcttgatt
     1561 tggataatcg ttgtgagcgg gatttatact taataactag tggtaattat aatccgagaa
     1621 tacacaaaga taatatcgcg gatgccggtt attctatgcc tgataagata gtcatggcta
     1681 cgaggcttct tggattgaat gcttatgtcg ttgaagaaag taacattttt tcgcaagtaa
     1741 ttagttttat ttatcccgat gcaagggact tattaattgg catgggatgt aatatagtac
     1801 atcaacgaga tgtgttatct tctaaccaaa gagttttgga agctgttgct gtatccttta
     1861 ttggagttcc tgtcggcctc cactgggtgt tgtgtagacc tgatggaagc tatatggatc
     1921 cagctgtagg agaaaattat tcttgttttt caactatgga attaggtgca agaagaagta
     1981 attctaactt tataggatat actaaaatag gtatttccat agttataact aatgaggctt
     2041 tgtaaattgt cgttttttat ctgtgaagga gcttgaatat tttggggcgg ggggcataac
     2101 aaatgatatt actgagtaca aatttagcta tattgggcgg attagttgtg tgggaagacg
     2161 aaatgtttct cttgaaataa gtgaactccc tcaaggaatg attgtgtgct taaatattta
     2221 agtgctggta agtttgagaa ggctgacata ttttgcagag caaacagtga ttgacttgga
     2281 tgatattttt attttagtca ctcgttgtaa ttggagtatg ctttatttta tttgaaaata
     2341 gatgtatttt atacccgcaa tttaaactgt aagccctatg aggggtatgg atattataag
     2401 ttactctttg actaagcata acttttgggg ttagaaaaat aaatctcttg ttaataatta
     2461 atataatttt aaaatcaaac ttgtttataa ttataaaata atgtaagtcg gtcattttat
     2521 gggaagatta ggagaataaa acagtaatta tatttgagaa atctgctaat atattcttaa
     2581 agcttttttg atactgtgaa cgaagtctgt taatggtggg ttttttgttt atcaccatgt
     2641 aaaaaaaatt gagtagtaaa agcgttgact gctaatcctc gctgttttta ttgaaacgat
     2701 taaaaatacg ctgaatattt tcggccagtc actcatcttc acataaaatt ttgaccatca
     2761 tgccgcatcc cttagttgca caattcattt tcaatgatat gaatattact aatttcacca
     2821 gataattttc tcgtggtcag cagggtatga gttttcaccg taattttaag gaaacacagt
     2881 gcgtggggat tgaataacag atagccgacg gaattacccc accggctgaa agtcaagttc
     2941 aacgaagatt tttattatct gaattgggca gtttacatca gtgttcgatg tttatgaaga
     3001 cgatagatgg cgagaaatta tatcccgaat agcttcatct atttctctct gaactgtttt
     3061 gtttatttta gataatgtaa atattagagt tctgtttgtt ttattctctt ttctttttat
     3121 gtaagtccgt ttgtctttag aaataaataa cgagtcaact tttagcgaag ggttttgctt
     3181 tctgtttttg cttttcttta tgatgtttaa aatttctttt ttatatatgt ccgggggcaa
     3241 atttgtatca aggtctttta tttcttcctt taatattggt agtgtagaac taagagattc
     3301 attagccttt tcaagtcctt tataataatt gaataatatc ttgtagtcat taaagttaag
     3361 ttctgacgcg attggaaata gagatattat ttcctgtgga acgcttgctg cctgaaaggc
     3421 acgagtgact ttcgcgcgag acagattctc ttttttggct atgtctttat aggacatccc
     3481 tgatactttc agaaaattaa gaccaatacc aagttctcgg atgctatgct cttttgctgt
     3541 ttgtatatcg tttgctagtt ttctggcatc aagagtagat atatattctt ttgaatatag
     3601 aacttcaaga tctgctcctg catatattgc agatgctctt ctacgagtgc catccagaat
     3661 ttcaattcta ccatcaatct cccttcctat tacagggaag aattgttgta gctttatagt
     3721 ttttatgata tcggctaatg attcttctgt tagggattct tgatcgcgta cattcgtttt
     3781 ttggttgacg aaggttaaat cttggatcct tttatgagga atcttggctt tgataaagga
     3841 aactttatta ccagaatgga gtgtaaactc tttcttttgg ccttccttta tacttaacaa
     3901 gtcgttgcac aaatccacca tatcacaccc tgtttattca tattgattta tattgacaaa
     3961 agttaaatgc agtgtgagca agattgtatg taactgttga tgatagtcaa cggtagtctt
     4021 aaaaattact attttaatag ctggattgat gcaatttatc tttattggat aaaagaataa
     4081 tattcttgtg gagctctgat gtgagagctc catagatgaa aaaatgctta tcaattacta
     4141 tattgatttt gtactatttt tgactacaga aataagatct ccaatagtaa taatgtgttc
     4201 taatgtagat tcatctatac gtagattgaa ctcaaaacta atcatatcca gcatttcaat
     4261 cagagaaaga gaatcggcag aaagatcttc aataaattta gtttcttcag atagtttgct
     4321 gtacgctata ccatagcaaa aggccactat tgataatatt ttttctttta tcatatttta
     4381 atccttattg atattcttta atacttttga tagggaagtg gttacatctt ttgctgcttt
     4441 atatatatta ttgttttcag ctgtaatatc attattattt tttaaatcag atatatcatc
     4501 tgtaagtaat tctgcagagg tatcgtcaat ttttgacaat accttgctga gggcgctaga
     4561 tacttctttt gccttttcat agatcgcatg atttttatca attgtatctg cgttggtttc
     4621 tgttacgtag tttcttatat ttgcatctgt aacttttaat ttgttgttta ttctatgttc
     4681 tgttgtggat tttgtaatgt cctttgattt ttcctgtaac tctcgttctt gtgttcctga
     4741 tttagttcca atgagagata taagattact tagggagttt gttacttttt ttgaagcttc
     4801 aaatatcgtt tcccgtgtta aatatgtgtc tccatttctt gataaaaaat cagtctgaag
     4861 tacttttgaa tcagtgtttg agttgaattt attgatttgc gaagctactc tatgagaaag
     4921 agaagcgcta cttttagttg tatctgatga gttatcatta ctgcttgtca ctccttccgg
     4981 gtggagaggg ggggtggttg aagagttctg tatgttcaga ggcctgtgtc cccgagaaag
     5041 agagttattt gcagatcttt cttgactact cgtagttaac aggtctgaac ctattccatc
     5101 cgaattatct ggagtatggt tgaaaacctt ttctaaagct gaattatttg caaatgtctg
     5161 gacagtatct gatatagagt gtggtagaac ggaagttact ttttgaacta gtgctctttg
     5221 tggatttagt aatgagtttc cgttacggga taatagatcg tttgtctgag aagtaggaga
     5281 ctgtgcatca ttttctgggt tctcgtgata gctattgtca tatactctgt tgtcaaaaac
     5341 tctattatcg taagttctat tatcattatt tatatgatag tgaattacag gttgggatgt
     5401 tttctcattg gctccggggg ttggtccgcc atctggcata tcttcgggag gtgtagggag
     5461 gccatcttgg ataggatgcg aatcttttat atcttctaaa atatcattaa ggacttttct
     5521 caacagctca ggaaacacgg ctactgtatt taagtctctt gacagtttac ctataccaaa
     5581 tcctttacta tcaatagtat ttttacgttg attgcgcaaa ctatcaaagg caagttttgc
     5641 ctgtgcgtca actaaatcat ctagtttgag agatgaaaca gtttcattac ttaactttaa
     5701 ttcagcaaga atattattct tgataaatga tgatacttca gtattgagga gtttttgtcc
     5761 caatgataaa gcttcaccag atgtgtgttg ttgtgctaat agtgcaagtc tgtgctcaat
     5821 gttttgtcgt aattgatgga gtttcttatt ctttgccgac catggtccaa aggcagaatc
     5881 ttgggataat ttatctttta taattagttc cgaatactgt gcgacaattt tttgaagtcc
     5941 cttttcgttt tcaaaattaa gaccattttg atgtactaag gagtacaaaa catttgaaaa
     6001 ttcgagaatc tcgcttttgg catacgcatt cattgagttg gaataaatgc ttcttaacac
     6061 atcttctttc cctgaggaag aaaataaaaa atcaagacat ttctgattaa aagacgttgc
     6121 aaagttttct ttttcagaca ctgatgctgg tgtttttaga gaagtttgcg aactatggat
     6181 atcggatatt ttgcttttta actcgctgta ttctgttgat gatggtgaag ttgccttata
     6241 taagaatgtt ggcgcttgag tattatttac attatgcatg gcgcacctca gaaatggaga
     6301 aaaagtttat ctgtatctgt acatgagctt attgtactac tcaaaacctt tactaaatta
     6361 tcaaaaatac tattggcatt actgtatttt tgaactaaag tttgaagatt atttttcatt
     6421 gtttcatctt cggcagagaa tccggcattc catgcctgat attttgcatt atctagcaca
     6481 acctcgccat ttccacctag attctctaag ctttttaaca tattgtctat tggggtcatg
     6541 tttatactga caacatatcc cccgtttttt tgagatacct tgccgattgt tccacctaat
     6601 tctgtaagcc atttatttgc ttgttcctga ctaacagtat tatttgctgg atatagcggt
     6661 ttatctttat atttttcctt gagttcttcc aatgcctttt taagcgagtt gacttgtaat
     6721 ttcacggagt ttccgtcgtt acctccggga gagatccagc cggcaagact ggaaagaaca
     6781 gcgctaaaat cttgatacat ttgagtatat gaactaacgg catgttcata tactttcaga
     6841 tactgttcat taatattatt gatggagttt gcaattttag cccacagttc tctatgagat
     6901 atcatttgat ctccatcaag ctcggcttct ttcggagctg aatgtaataa ttctcttgcg
     6961 tctttattaa ttggatatcc gttcctggaa agaatattca atagttgagc ggatttattt
     7021 acatccatgc taatctgaga tgaatgtaat gctatttctt ctagcgatgt tttagtcaac
     7081 gttttttgtg aaagctcttt ctttaatgcc tgatttgttg ttctgatatt atgaagggtg
     7141 tcgttgagca tagtaaggga acttacagga tgagaactgg tcgttgtttt tatatcagaa
     7201 ttaactgttt cggttgatga accgttggta ttgtttggac tgaatgatga ggtggaaata
     7261 ctattagtca gagttgttat attcatggtt atttccttac aggaagagcc atatattatt
     7321 atggctcttg gatatattaa gctcgaatgt taccagcaat ctgactggct gtcgaattct
     7381 ttgattggtt gatgctgtca attatattca ataatttttg tattaattga tttgtcgctt
     7441 gggatgcttc tttagatact tgggatgctt cctctgcttg tttactgctg gcctgactga
     7501 ttagttgttc ttcttcttca agagcagatg cataacgccc tcctgatgtg gatatattac
     7561 cagcaacggc tgatgtttcc attgttgcac ggccaatgtt ttgtttttgc tgcgcagaaa
     7621 gggtattgag ctcgtaagtt cttctctggg tgtcaatttt atcctgcaaa gaaatatcgg
     7681 gagaactaag agaagtttta tgttcagttg acagggatat attatctggc gccagtttat
     7741 ttttacctaa aaattttgtg ctagagttag tttgtggtga ggtgatattt gtatctattt
     7801 gtttatttaa ccctaattta gaacctgcaa gctctttttc aagagattga gcagtggcaa
     7861 ggttctttct taaggctcct ttttggtcgc taatccctga atgcgttttt ttggcaccga
     7921 tacccgttat acctacttgt gtgactgctc cagtaatgct tgatgatagg gctgccaggc
     7981 cttgccgaac aatgttctct gcagctgatt ttgtagcatc gaacgcaatc aatgacaatt
     8041 gagagcccaa tttagtttct gcagtgcgga gagcagaaag tagaacggct acactaataa
     8101 ttaaagaaac agcattagaa gaaagactgg aaatatccag agtgttctct ggagtaaggg
     8161 tgtgactcat ctctgaaaga tctttcatct ttttgtcagt aaggcgagca atgatttctg
     8221 aatcatgctg aacctgctct gataacttta aaagttgatc atcatttaat gtggttgtta
     8281 atacgggatt tttaccgaca ttaagtggaa tatgcgctgc aatctgctga taattttgtg
     8341 atttttgtgt ttcggaagaa ctttgagttt gttttgtgga tatatctgta tataaaatct
     8401 gggttggttt tgtgttttga atttccataa cattctcctt atttgtatca agcagtagtt
     8461 tgttgcaaaa ttgcttttgc aacatcagtt ctattagcct gagagttgga cattgaggct
     8521 aatagatctg caattacttc ctgcaattgg ccgaattttt ctattgcttc actgatatat
     8581 tttgacagtt gttcaacttg atatttcgat aatgtcaggt ctgctagatt tgtcgacgcg
     8641 ctgttctgga aaacagcaga agcgacactt cctcccgctt gtgtggcaga gttaacactt
     8701 tctcctaata aaactgcttg gtttaaatgg gtggaaataa tttgtttgga tattacttca
     8761 tcacccgctg caccaagaaa tttatttaat ctggcaacag cattagtgat taaatcgtcc
     8821 agttgagaag aaaaattctt gagaaacttt ggtataaggt ctgtgagggt tttacctatt
     8881 attttgccaa tattttctgc aagttttgct gctgcctgtt taccaacagt ggctacgaga
     8941 acgactgctg caactaggac aagagcgcct gcgattgccc ccagaataga gccaatcatt
     9001 ttggcttttt tcgagtcgac gcccaagcct tcgagcattt ttgtaaatgc atctgaaagg
     9061 agtttgatta agggttcaat gactgctttc atgatcggat tcagggcttg ttccatgaag
     9121 gaattgccgg tcgctgcttg tactatagca tccgtaacca taagagctaa accaacagct
     9181 gccagtgcta gagaggctcc tccagaaaaa gctgctgcaa caacactaac gatagttaaa
     9241 agtgccccaa gtattttccc aacacaaccc attactctgt tgagttcttc tgctttacgt
     9301 acttcagcag catactcatc agattttctc tccatttcag tttttcttga ttcttggaga
     9361 gactggaata gagccagatc attttttaaa gattcttcat tattttttcc aactagttga
     9421 ataaaggttg ccatcaattg agtaacactg gcaagtccgg ttaatgattt ctgctgggtt
     9481 gatagctgtt cagcagatgc tgtgtttgaa aatgcagaaa aagagtctat ttctttttcg
     9541 agttgcattg atttatctgt aagtttgcta tgaattgaca gggttttctg ctcaatcaat
     9601 gtcctgtctt taactgctgc gtcttttttg atagtgagtt gtatttcttc ccggcttaat
     9661 tttttctttt ctggtgactc tgggtcgagt tcggataatc ttgtttgaat ttggttaatt
     9721 ttattttcta ggtcttttat tttagaatct gcgtttttta gtttattaat ttgtttttca
     9781 tagtctctgg ttagtccttc agtttcagat agaagagtgt taattttatc ggagaattct
     9841 aggttttttt gctgtcttgc ctgttgctgg gacttccaag cagtaatttt atttgttaat
     9901 gcagttaaag atttttcacc gagtatttga ataaggtttc caattaaaag cgttagttgg
     9961 gaacttgcat ttaatgactt tggtgcttta agttcaggga ttaatatatt tgaagttgag
    10021 tgtgcattag ttgtattaat attttggtta gcagtaagat cagcaattgt aagagaaaat
    10081 aatttgttag ctgcatcatt tgcagcttgg atagtattgt ctccaagctc agtggaagcc
    10141 aatattttgg caagaggaaa accagtggtt gtggtgctta cattatgcat aataattact
    10201 ccttgatatc ctgaattgcg tccaagtatg attgtgcttt tatttttaat ttttcatcat
    10261 tgctgtgttg aattacgagt tcgaagcact ctttagcttt taagggggct ttcaaccgaa
    10321 gctggcattg tccagtatgg aatactggtg tatagtcatt ttttcctaat gcaaaagcga
    10381 cagcataaag gtctgctgct tgttggaact gttcttttat ctgataaata gctgcgagtc
    10441 ccataatgta gtctacattg taaaagtcgt atatacataa aaacctgaag aaaacttcag
    10501 cttcctctat tcttcctttg ttgtaaaagt cataagcata tgaataaatg tcatccatca
    10561 tatcatcagg aattgcatta atatctttca gtgtagcgcc agagttaatt gcatcaatta
    10621 ctgcagtaga gatgctttca ttttcggtga tatttaaaga cataaggtct cctttttgtc
    10681 tgctttactt taataagtat aagatttaat ataaaagatt taatttgtat tgctttgacg
    10741 gtatacagcc tttagaacct ctgcgcttat ttctatttgt aaaacttttg ttgattcatt
    10801 aaatatttta tcgtagacgg aactggatac tagatttgta agatccttca tttgtgtgtt
    10861 aaacaaatcc atgtcactgc tatagcgttt taaaatcttt gctctgaggc cagatgaggc
    10921 aatctcgttt agtgctgtat gactgataga tgtttgtgct gatttttcat caagttttat
    10981 atccgggaat tttttttcaa tagctgcaaa tatgtttttt ttgttatctg aggtcaaaac
    11041 aactccattg tgtatggcca tttcatctat acttctgttt atcattgaag agacataagt
    11101 ggttcgctct tggctcatcc aacaaaatag ttgattcccc gaattactat cttttatttt
    11161 gtctatgacg ctgtccctga ttttatttat attcaccggg catccaaaat tcttctgagg
    11221 aaagcgttgt tttaagttaa aaacagataa tattttcttt acaagcttat tgcgacttaa
    11281 cgaatcaaat gatgggcgag gaatcgcgaa ttcaacctgt ggaagtattt tgtttagaat
    11341 ttgcatgata ccccctatat gttagttcac ttctgaagtg atgtttgcaa tggttagttt
    11401 aatacaaaat aaaaattcat acaaagattc aatgagttca tcaggtgaaa gatatttttt
    11461 gtagtgtttc ttggtcaaaa cacattgatg agtatcaggg agaagacaga tagaagaacc
    11521 ataatctcta ctcaaagagt agttaaggat gagtatgtgt tctggattaa taatattttc
    11581 tgtaaaaagt tcatgttcat taagaagtcc ggtcaaaaag atgtattcat cattatctgt
    11641 tatacaaaca ggtatttgtt cttcgagcaa tataaaggct tgatttttat cgttaaaatc
    11701 aagctttgca cctgttattt cataaagttt atcatatagt ttgcgacaca tttctatggc
    11761 cttctatata ttagaatgag agttattcaa taaaagctta ttgatctgta ataagtcgtg
    11821 aatgtaaagt ttggttccaa gatctggcga tttaagagaa ttgtaataat caataatttt
    11881 tattgatttt tcgatgattt cttctttttt atctgttgct tcggttttta attcgtttaa
    11941 tacacgagtc atattgctgg gtgctgattt tactaagtat gagtggcaag cgttgatgac
    12001 cgcatcaaag tcttctgaag atacattttc agtaggatgt ttgtaaagtg aaattgataa
    12061 aggcttgaat gtttttggtg tgtggataag gttaatttta ttgaaacttt taagatagct
    12121 gcgtcgtaat tgagtcaatc tggaaagatc aaaatcagat tcaatatact tcattatttc
    12181 gttataagac tgattgagac tttcaatgcg ttgcctaact ttatttgcat acgcgtatac
    12241 atcgttaggt gtagcaacca atttaacgtc agggagtgga aagtatattt cagaatttcc
    12301 tgctttaaga acattaagtg ccataccagc acagttttca gatttactga ttaatttata
    12361 atttgccaat tgtctgaaag catctgcttt ctttttgcat ataaattttg acatgtttgt
    12421 ttcatcaagg ccaaaaaggt tatggctgat tttaccatcc tttgttttat tatcacctga
    12481 taatggaatg tatactttat cagcactgac cccccaataa ccataggggt cttttttttg
    12541 attagctctg ggaaaaaata tttttttgtt gttattttga tcttgttttg aatttaataa
    12601 gtttctaacc tcttggcctg catcaagtct ttcggctgtt tggtctgaaa tcatatttag
    12661 tttgtcttgc ttataggtat cgacactgta acctgagcgt ttttcaaaaa ggtagtttat
    12721 ttctgtatct tttgtaacat tcccgccggg ataccagctg agataggtat gagttggttc
    12781 ctgtagtaaa ttgtttttta tactgatact agtatgccct aaatgggtta tgcctctatt
    12841 tccccagcaa cgaaaaacta aatgtctttc aagatttttg gctgtaccga tgccatgaaa
    12901 acgctctgcg gtattcaaca ttaatttgat gtctctacct gtgaaagagt gttgaattga
    12961 gtttttagct gcttcatagg cggcaaacgc actttctgca gatttttcgc tagctaatgt
    13021 attgaggaaa agcaataagg atttttgatt ctctagtcga taatttttta gttgttcatt
    13081 taatatctgt gaatttatct ttgcattaat agtgcttaat gttctgaaaa agagatcctt
    13141 taggttgaat ttctgtgcta tcaatatagg tccatgctct gtatcttcaa cgcgtatagg
    13201 cccttttgta ttgcttgcgt caatgaaatt gctaattttg aggatcatac tttattaact
    13261 ctccattact tggtgattgt ggggatgtaa aatgtatttg gtatataata tattcaatta
    13321 ataaatttag aaacttgagc ctgttaacat aatcaaattt tcttttgctg tacataatat
    13381 gtacctcgtg agcatatgta gtgctcgttt catcatgaaa tcccacaaga taaagtgcct
    13441 gatgtatcag gctcggagtg ttatagaaaa agagagaacc ctgttgaata agtatctttt
    13501 ttgttgtaaa acgaggtaca aaactgcaat caatgcccca tttatgactg aggataatta
    13561 aatgcacata actaatttgg gattgcatca ggtttcattt caaagcggag attcctataa
    13621 aggcgcagaa gaaaccggaa agcacaaagg tgtaagcgtg atttcatatc aaagagttaa
    13681 aaatggagaa agaaataagg ggatcgaagc gcttaatcga ttatatttac agaaccaaac
    13741 aagcttaaca ggaaaaagcc ttttgtttgc gcgtgacaga gctgaagttt tttgcgaggc
    13801 aataaagttg gcaggtggtg atacgtcaaa aattaaagcc atgatggaac gattagatac
    13861 ctataaactt ggtgaagtta ataaaagaca tattaatgag cttaataaag taataagtga
    13921 agagataaga gcacagctag gcattaaaaa taaaaaggaa ttgcaaacga aaataaaaca
    13981 aatatttact gattatttaa ataataagaa ttggggaccg gtcgacaaga atattagtca
    14041 tcatgggaaa aattatggtt ttcaattaac tcctgcctct catatgaaga tagggaataa
    14101 aaatatattt gtcaaagagt ataatggaaa aggaatttgt tgtgcttcta cgagagagag
    14161 tgatcatatc gcgaatatgt ggctttcaaa agtggtggat gacgagggga aggaaatttt
    14221 ttcgggtatc agacatggag ttatatcagc ctatggattg aaaaaaaact catctgaaag
    14281 agctgttgct gctcgtaata aagctgagga gttagtaagc gcagcattat atagcagacc
    14341 agagttatta tcacaggctt tgtctggtaa aacagtagat ttaaagattg tttcgacttc
    14401 tcttctgacg ccgaccagtt taaccggggg agaggagagt atgttaaaag atcaggtgaa
    14461 cgcattaaaa gggttaaact ctaaaagggg ggagccaacg aaattattaa ttcggaatag
    14521 tgatggtctt ctgaaagaag taagcgttaa tctaaaagtg gtgacattta actttggtgt
    14581 aaatgaatta gcgctaaaaa tgggcttagg ctggaggaat gttgacaagc ttaatgatga
    14641 atcaatatgt tctttgttgg gggataattt cctcaaaaat ggtgtgattg gaggttgggc
    14701 tgcagaagca atagaaaaaa atccaccatg taagaatgat gtgatatatc tggctaacca
    14761 gataaaagag attgtaacca aaaagttaca aaaaaatgat aatggagagc cgtataaatt
    14821 gtcacaaaga atgactctat tggcttatac tataggtgct gtaccttgct ggaattgtaa
    14881 gagtgggaag gacagaacag gtatgcagga tgctgaaatt aaaagagaaa taataagaaa
    14941 acatgaaaca ggtcagtttt ctcaattaaa tagtaaatta tcctcagaag agaaaagatt
    15001 attttctact attctaatga atagtggtaa tatggaaatc caagagatga atactggtgt
    15061 gcccggaaat aaagtcatga aaaaattgcc gctatcttcg ttagagctat cttattctga
    15121 aagaataggg gacccaaaaa tatggaatat ggtgaaaggg tattcgtcat ttgtataaga
    15181 ggaatatatg gaagatttag cagatgttat ttgccgagcc ttgggtatcc ctttaattga
    15241 tatcgatgat caagcaatca tgcttgatga tgatgtgcta atatatattg aaaaagaagg
    15301 agattcaata aatttattat gccctttttg tgcattgcct gaaaatataa atgatcttat
    15361 atatgcgttg agcctaaatt actcagaaaa gatatgctta gctactgatg atgaaggcgg
    15421 aaatctaatt gcgcgattag atcttaccgg gataaatgaa ttcgaggatg tatatgttaa
    15481 tacagaatat tatatttcgc gtgtgcgttg gctgaaggat gaatttgcgc gaagaatgaa
    15541 ggggtattaa tgtcccgttt cgtatttatt ttgctgtgtt ttaccccata tttagggaga
    15601 gctgattgtt gggataaggc tggtgaaagg tacaatattc catcaagttt gttaaaagcg
    15661 attgcggaaa aagaatccgg atttaataaa tctgcagtca atgttaataa caatggaagt
    15721 aaagattatg gtataatgca aataaatgac tttcattcta aaagacttag agaaatggga
    15781 tattctgagg aaatgctaat tagtcatcca tgtctttctg tacattatgc agcaaagtta
    15841 ttaaatgagt ttatgatgat gtatggaaga gggtgggagg ctgttggagc ctataatgca
    15901 ggaacgtctc cgaaaaagaa aaaagaaaga ttaaagtacg cagaggatat ctatagacgc
    15961 tatttgcgga tagcagcaga gagcaagcag aataatcgaa ggatataaga ggatttatgt
    16021 ctgaggcaaa gaactcaaat cttgcaccat tcaggttatt agtgaaactc acaaacggtg
    16081 tcggggatga gtttccgtta tattatggga ataacttaat tgttctcgga cggacgatag
    16141 aaacacttga gtttggaaac gacaatttcc cagaaaacat tattcctgta acagatagta
    16201 aatcggatgg tataatttat ttgaccataa gcaaagataa tatctgtcaa tttagtgatg
    16261 aaaaaggtga gcaaattgat atcaattcac aatttaattc atttgaatat gacgggattt
    16321 cttttcacct taaaaatatg agggaggata aatcgagagg ccatatacta aacggaatgt
    16381 ataagaacca ctcggtgttt tttttctttg ctgttattgt tgtgttaatt ataatttttt
    16441 ccctctcttt aaagaaagat gaagtaaaag aaattgctga aattatagat gataagagat
    16501 atggaatagt caacactgga cagtgcaatt atattcttgc cgaaacacaa aatgatgcgg
    16561 tatgggcgtc tgtagcctta aataaaaccg gatttaccaa atgcagatat attctggtgt
    16621 caaacaaaga aataaataga attcaacaat atatcaatca acgattccca tttatcaatt
    16681 tgtatgtgtt gaatttagtg agtgataaag cggaattact ggtgttttta agtaaagaaa
    16741 gaaatagttc aaaagatacc gaacttgata aattgaaaaa tgctctgatt gttgaatttc
    16801 cgtatatcaa aaatattaaa ttcaactatc ttagcgatca taatgcaagg ggggatgcta
    16861 aaggtatttt cacaaaggta aatgtccagt ataaagagat ttgtgaaaat aataaagtta
    16921 catattctgt cagagaagaa ctgacagatg aaaagcttga gctcattaat agactgattt
    16981 ctgaacataa aaatatatat ggagatcagt atattgagtt ttctgtactt ttgatagatg
    17041 atgattttaa aggtaaatca tatcttaaca gcaaagacag ttatgtaatg ttgaatgata
    17101 aacactggtt ttttttagat aaaaataagt gaggataaaa tgagtgttac agtaccgaat
    17161 gatgattgga cattgagttc attatctgaa acttttgatg atggaactca aacattacaa
    17221 ggtgaactaa cattggcact agataaatta gctaaaaatc cttcgaatcc acagttgctg
    17281 gctgaatacc aaagtaaatt atctgaatat acattatata ggaacgcgca atccaataca
    17341 gtgaaagtga ttaaggatgt tgatgctgca attattcaaa acttcagata atagggagca
    17401 ttcatgaatt acatttatcc agtcaatcag gttgatatta tcaaagccag tgattttcaa
    17461 tctcaagaga tatcaagtct ggaagacgtc gtgtcggcta aatatagtga tattaagatg
    17521 gatacagata ttcaagtatc acaaataatg gagatggtaa gcaatccaga atcattaaac
    17581 ccagaatctt tggccaagtt acagacgacg ctctcaaatt attcaatagg agtatcatta
    17641 gctggcacgt tagcaagaaa aacagtttcg gctgttgaaa ctttattaaa gtcttaattt
    17701 atatgattag gtataaaggt tttattttat tcttgttgct gatgttgatt ggatgtgagc
    17761 aacgtgaaga gttaatttct aatttatctc aaagacaggc aaatgaaata atatctgtgc
    17821 tagaacgcca taatattact gctagaaaag ttgatggagg taaacagggg atctcggtac
    17881 aagtcgaaaa ggggacattt gcatcggcag ttgatttgat gcgcatgtac gatttgccaa
    17941 atccggagag agttgatatc tcacaaatgt ttcctacaga ttcattagtg tcttctccaa
    18001 gagctgaaaa ggcccgttta tatagtgcta ttgagcaacg gctggaacag tctttagttt
    18061 ctattggtgg tgttatttcg gcaaaaatac atgttagcta tgatcttgaa gaaaaaaata
    18121 tatcttcaaa accgatgcat atatcagtaa tcgctatata tgactcaccg aaagagtctg
    18181 aactattagt tagtaatatt aagcgatttt tgaaaaacac cttttctgat gttaagtatg
    18241 aaaatatatc tgtcatatta actccgaaag aagaatatgt ttatacaaat gtacaacctg
    18301 ttaaggaagt taaatcggaa tttttaacaa atgaagtaat atatttattt ctcgggatgg
    18361 ctgtactagt tgtcattctt ttggtatggg cattcaaaac agggtggttc aagagaaaca
    18421 aaatatgata agaatggatg gaatttataa aaaatatctt tcaataattt ttgatccagc
    18481 gttctatata aatagaaatc ggttgaattt gccttctgaa ctgttagaaa atggcgtaat
    18541 cagaagtgag attaataatc tcataattaa taaatatgat ctaaattgcg atattgaacc
    18601 tttaagcggg gtaaccgcta tgtttgttgc caactggaat ttacttccag ctgttgctta
    18661 ttttatcggt agtcaagagt ctcggttaat taatcatagt gagatggtta ttagttatta
    18721 tgggggcaaa atcagtaagc aaggggaggc cgctattcga agtggttttt ggcatcttat
    18781 tgcgtggaag gaaaatatat ctgttggtat atatgaaaga ataaacttat tatttaatcc
    18841 aattgctttg gagggaaatt atacgcctgt agaaagaaat ttgtcccgat taaatgaagg
    18901 tatgcaatat gcaaaaaggc actttaccgg tatccagaca tcatgcctat gatggtgttg
    18961 ttataaagag aattgaaaag gaattatgta aaaccataaa agatagagac actgagagta
    19021 agaagaaagc aatttgtgtt ataaaggagg ctacgaaaaa agctgaatca ctgcgtatag
    19081 atgctgtatg tgatggttat caaattggta tacagactgc ttttgagcat attattgact
    19141 atatttgtga atggaagtta aagcaaaatg aaaacagaag aaatattgag gactatataa
    19201 ctagtttgct ttcagaaaat ttacatgatg agaggatcat tagcacttta ttggagcagt
    19261 ggcttagttc gttgcgtaat acagtaacag aattaaaagt ggttttgccc aagtgtaatt
    19321 tagcactgag gaaaaagtta gaacttgatc tccataaata tcgctcagat gtcaagatta
    19381 ttttaaaata ttctgaaggg aataattata tattctgtag tggtaatcaa gtagtggagt
    19441 ttagtcctca ggatgtaata tcaggtgtta aaatagaact tgcggaaaaa ttaacaaaaa
    19501 atgataaaaa atatttcaaa gagttagcac ataagaaact gagacaaatt gcagaagact
    19561 tgttaaaaga gaatcctgtt aatgattaat caaataaatg caagcaatgc tctgcagcaa
    19621 agattaaata gtgaagaatt tgttaattta aatgagagat tatcatcttc gcaatcattt
    19681 gatgaggata taatctatga aataatgcaa tatttctcac agagtgaatt aaattccata
    19741 gataatgatg aactgcacaa taagattgaa caattattca attcacgttt tccttatctt
    19801 acagctgcac aaaaaagttc acttcttaat aaactcatag atgcaaatca atatgtcgat
    19861 ttgcatgagg gattttatgc atcattgtcc atatataaca atattgattt ctatattaag
    19921 actacaacct tcgattcatt aattagtgtc tttgaagcag ggagagaggc agatgattcg
    19981 acatggtagt aataagttga aaatatttat tttaagtata ttgctattaa cactgagtgg
    20041 gtgtgcttta aagtcatcat ctaattctga aaaagaatgg catattgttc ctgtaagtaa
    20101 ggattatttt tctattccaa atgatttatt atggtcgttt aatacaacca ataaaagtat
    20161 aaatgtttac tctaaatgta ttagtggtaa ggcggtttat agttttaatg caggtaaatt
    20221 catgggcaac tttaatgtta aggaagtaga tgggtgcttc atggatgcac aaaagatagc
    20281 tatagataaa ctattttcta tgctgaaaga cggggttgtt ttaaaaggta ataagataaa
    20341 tgataccatc cttatagaga aggatgggga agttaaatta aaattaattc gagggatata
    20401 attgtattgt gagtaaatat aaaggtctaa atacaagtaa tatgttttac atttactcta
    20461 gtggacatga accagttaac gttgagcttg taaaagataa agaacgtaac ataattgagc
    20521 tggctccagc atggaagggt ttttttttgt ccgaaatcaa aatataaaat tcagtgataa
    20581 tgtgaactat cattatcgct ttaacattaa tagttgtgca aaatttcttg ctttttggga
    20641 ctatttcagt ggtgctctag tagagcatag ccatgctgaa aaatgtattc atttctatca
    20701 tgagaatgat cttcgggata gttgtaatac cgaatcgatg cttgataagt taatgttaag
    20761 attcatcttt tcatctgacc agaatgtaag taatgcactg gctatgatac gaatgactga
    20821 gtcatatcat ttggttcttt atcttctgag aactattgag aaagaaaagg aggtaagaat
    20881 aaagagtctg acagagcatt atggtgtgtc ggaggcttat tttagatctt tatgtagaaa
    20941 agctttaggg gcaaaagtca aagaacaact gaacacttgg cggttagtaa atggtttatt
    21001 agatgtattt ttgcacaatc aaacaatcac ttcagctgcc atgaataatg ggtatgcatc
    21061 aacatcccat ttttcaaatg agataaaaac acgactaggc ttttctgcaa gagaattatc
    21121 gaatattact tttttagtga aaaaaataaa tgaaaaaatt taatattaaa tctttgactc
    21181 tcttgattgt attgttacca ttgatagtta atgctaataa tattgactct catttattag
    21241 aacaaaatga catagcgaaa tatgttgctc aaagtgatac ggtaggatct ttttttgaac
    21301 gtttttctgc gttattaaat tatcctattg ttgttagcaa acaagctgca aaaaagagaa
    21361 tatctgggga gtttgattta tctaatccag aagaaatgct tgaaaaactg acattacttg
    21421 tgggactcat atggtataaa gatggaaatg cattatacat atatgactct ggagaattaa
    21481 taagcaaagt tattttgctg gagaatatat cacttaatta tcttattcag tatctgaagg
    21541 atgctaacct ttatgatcac agatatccta taagaggaaa cattagtgat aaaacattct
    21601 atatatctgg tcctccggca ttggttgaac tggtagctaa tacagcaact ttgcttgata
    21661 agcaggtttc ttctattggg acagataaag ttaattttgg ggttataaaa ttaaaaaata
    21721 catttgttag tgacagaact tataatatga gaggggagga tattgtcatc ccaggggtgg
    21781 ctactgttgt agaacgttta ttaaataatg gtaaggctct gagtaacaga caagcccaaa
    21841 atgatccgat gccacccttt aatattacac agaaagtatc tgaggatagt aatgactttt
    21901 catttagttc agttaccaat agttctatat tagaggatgt atcacttatt gcatatccag
    21961 aaaccaattc tatattagtc aaaggtaatg atcagcaaat acagattatt cgcgatatta
    22021 tcactcagtt agatatagcc aaacgacata tagaattatc attatggata atcgatattg
    22081 ataaatctga attaaataac cttggtgtta attggcaagg aacggcttca tttggtgatt
    22141 cgtttggtgc gtcatttaat atgagctcat cagcatcgat aagcactttg gacggcaata
    22201 agtttattgc ttcagttatg gcattaaatc agaaaaagaa agcaaatgta gtttcaaggc
    22261 cggtgatttt gacacaagag aatattccgg ctatatttga taataacagg actttttatg
    22321 tgtcattagt tggtgagcgc aattcttcgt tggagcatgt tacgtatgga acattgataa
    22381 atgttatacc tcgcttttca tctcggggac aaattgagat gtctttaacg atagaggatg
    22441 gaacaggaaa tagtcagtca aattacaatt ataataatga aaatacatct gtccttccag
    22501 aagttggaag gaccaaaatc agtacaatag cgagagttcc acaaggcaaa agtttgttga
    22561 taggtggtta tacgcatgaa acgaactcta acgaaataat atctattcct tttctctcct
    22621 ctattcctgt aatcggtaat gtgttcaagt ataaaacaag taatatcagt aatattgtaa
    22681 gggtttttct gattcaaccc cgtgagataa aggagtcaag ttattataat actgcagaat
    22741 ataaaagcct catatctgaa agagaaatac aaaagactac acaaataatc ccatctgaaa
    22801 ctactttgct ggaagacgaa aaatcattgg tttcatactt aaattactaa ctataaagta
    22861 ggtgatgtat gcttgatgtt aaaaatacag gagtttttag ctctgcattc attgataagt
    22921 tgaatgcaat gacaaattca gatgatggag atgagactgc tgatgcagag cttgattctg
    22981 gcttggctaa tagcaagtat attgactcat ctgatgagat ggcttccgct ctttcgtcat
    23041 ttataaacag aagagacctt gagaaactga aaggaacaaa tagtgatagt caggaacgta
    23101 ttttagatgg ggaagaagat gaaattaatc acaagatttt tgatttaaag agaacgttaa
    23161 aagataacct tcctctagat cgggatttta tagacagact aaagagatat tttaaagatc
    23221 caagtgatca agtcttagca ttaagggaac ttttgaatga aaaagatctt actgctgaac
    23281 aagtcgaatt attaactaaa attattaatg agataatatc aggtagtgaa aaaagtgtta
    23341 atgctggaat aaattcagct atacaggcta aattatttgg caacaaaatg aaacttgaac
    23401 cacagctttt gcgtgcatgt tatcgtggtt ttatcatggg gaacatatca acaacagatc
    23461 agtatataga atggcttggt aattttggtt ttaatcacag acatacaatt gtgaattttg
    23521 tagagcagtc actgattgta gacatggatt ctgagaaacc gagctgtaat gcttatgagt
    23581 ttggttttgt gttatctaaa ttaattgcaa ttaagatgat tagaacttca gacgtaattt
    23641 ttatgaagaa actggaatcc tcaagcttgc taaaagatgg cagtttaagt gcagagcagc
    23701 tattgctaac tttattatat atttttcaat atccaagtga aagtgagcaa attcttactt
    23761 ctgttataga agtatcacga gccagtcatg aggattctgt agtgtatcaa acatatctat
    23821 cttctgttaa tgaaagtcct catgatatat ttaaaagtga aagtgaaaga gaaattgcga
    23881 tcaatattct acgagagctt gtcacaagtg catacaagaa agagctttct agataacagg
    23941 agataaaagt gatccagtct tttcttaagc aagtaagtac taagcctgaa ttaattatat
    24001 tagtgcttat ggttatgatc attgctatgt tgatcatacc attacctaca taccttgttg
    24061 attttttaat agggcttaat attgtacttg ctattctcgt ttttatggga tcattttata
    24121 ttgaaagaat attgagtttt tctacattcc cttcggtttt gttgataacg acattatttc
    24181 gtctcgcttt atcaataagt actagtcgac taattttagt tgatgcggat gcgggtaaaa
    24241 ttattactac atttggccag tttgtcattg gtgatagtct ggctgtaggt tttgttattt
    24301 tttcaatagt aactgttgtg cagttcattg ttataacaaa aggatctgaa agggttgcgg
    24361 aggtagctgc tcgcttttct cttgacggta tgccggggaa acaaatgagc attgatgcgg
    24421 atttaaaggc tggaattatt gatgccgcag gagctaaaga aagacgcagt attctggaac
    24481 gtgagagtca attgtatggt tcatttgatg gagcaatgaa gttcattaaa ggtgatgcaa
    24541 ttgctggcat cattattatt tttgtcaatt taataggagg tatttctgtt ggtatgagtc
    24601 aacatggaat gtccctatct ggtgcattat ctacttatac catactaact attggtgacg
    24661 gattggtctc tcagattcct gctctgttaa tttctataag tgcaggattt atcgttacaa
    24721 gagttaatgg tgatagcgat aatatgggac gtaacataat gtcccagata tttgggaatc
    24781 cttttgttct cattgttaca tcagctcttg ccttggcgat agggatgttg ccaggttttc
    24841 cgttttttgt ttttttcctg atagcagtta ctttgacggc tttattctat tataaaaagg
    24901 tcgtagaaaa agaaaaaagt ctgtctgagt ctgattctag tggctatact ggtacatttg
    24961 atattgataa tagccatgac tcatctttgg caatgataga aaatctggat gctattagtt
    25021 cagaaaccgt tcctttaatt ttattatttg ccgaaaataa gataaatgca aatgatatgg
    25081 aaggtcttat tgaaaggata agaagtcagt tctttattga ttatggtgtc aggcttccaa
    25141 ctattttata tagaacaagc aatgagctta aggtcgatga tattgttttg ttaataaatg
    25201 aggtgcgtgc cgatagtttt aatatatatt ttgacaaagt gtgtattaca gatgaaaatg
    25261 gagatataga tgctctaggt attcctgttg tttcaacttc atataatgaa cgtgttattt
    25321 cttgggtaga cgtttcatat acagaaaatc taactaatat tgatgctaaa attaaaagtg
    25381 ctcaagatga attttatcac cagttgtcac aagctttatt aaacaacata aatgagattt
    25441 ttggtataca agaaacaaaa aatatgttag atcagtttga aaatcggtat cctgatctat
    25501 taaaggaagt cttccgacat gtgactatac agagaatttc tgaggtatta caaagattgc
    25561 ttggagaaaa tatttctgtt cgcaatttaa aacttattat ggagtctttg gcgctttggg
    25621 ctcccagaga aaaagatgtc ataacattag ttgaacatgt ccgtgcatca ctttctaggt
    25681 atatttgtag taaaatagct gtttctggtg agattaaagt tgtgatgctt tccggatata
    25741 ttgaggatgc aataagaaaa gggataaggc aaacctctgg tggctctttc ttgaatatgg
    25801 atatagaggt ttcggatgag gtaatggaaa ctttagcaca tgctttgaga gaattgagaa
    25861 atgcaaaaaa aaatttcgtt cttttggtat cagtagatat acgtaggttt gttaaaagac
    25921 ttatagataa cagatttaag agtatactcg ttatatcgta tgctgagatt gatgaagcat
    25981 ataccattaa tgtattaaag actatttagt gaggtttaaa tatgagtaac attaatttag
    26041 ttcaattagt tagagatagt cttttcacga ttggttgtcc tccatcaatt attacagatt
    26101 tagacagcca ttcagcgatt accatttctt tggatagtat gcctgctatt aatatagctc
    26161 ttgtcaatga gcaggttatg ttatgggcta attttgatgc accaagtgat gtaaaacttc
    26221 agtcatctgc ttataacata ttaaatttaa tgttaatgaa ctttagttat agcattaatg
    26281 agctggtaga gcttcatcgt tctgatgagt acttacagtt aagagttgtt attaaagatg
    26341 actacgttca cgatgggatt gtttttgcag agattctgca tgagttttat cagaggatgg
    26401 aaatcttaaa tggggtctta taatcaatga gctatacaaa attgctcact caattatctt
    26461 ttcctaatag aatctcgggg ccaatcttgg aaacaagtct tagcgatgtt tcgattggtg
    26521 agatttgtaa cattcaggct ggaattgaaa gtaatgaaat tgttgcaaga gctcaggttg
    26581 taggatttca tgatgaaaaa acaatattaa gcttgattgg aaattctcgt ggactttcac
    26641 ggcaaacgtt gattaagccc actgcccagt ttcttcatac gcaagtcggc cgtggattat
    26701 tgggagcagt agtcaatcct ttaggggagg ttactgataa gtttgctgtt acagataaca
    26761 gtgaaattct ttatcgacct gtagataatg ctcctccgct atatagtgaa agggctgcaa
    26821 ttgagaagcc ttttttaaca ggtattaagg ttattgattc tttactcacg tgtggtgaag
    26881 gacagcgaat ggggattttt gcgtcagctg gttgtggcaa aacttttctc atgaatatgc
    26941 tcattgaaca tagtggtgct gatatatatg ttattgggtt aattggtgag cgaggtcgag
    27001 aggttactga aacggttgat tatttgaaaa actctgagaa aaaaagcagg tgtgttttag
    27061 tatatgcaac ttcggattac tcttcggttg atcgttgtaa tgctgcatat atagccactg
    27121 ctatagccga attttttagg actgaaggac ataaagtagc gctttttatt gattcattaa
    27181 caaggtatgc cagagcatta cgtgatgtgg ccttagccgc tggagaatca cctgccagaa
    27241 gaggctatcc ggtttcggtt tttgatagct tacccagact tcttgaaagg ccaggaaagt
    27301 taaaggcagg tggctctatt actgcatttt acactgttct tttggaggat gatgattttg
    27361 ctgatccatt agctgaagag gtaagatcca ttttagatgg acatatatat ttgagcagaa
    27421 atctagccca aaaaggacaa tttcctgcaa ttgattcctt aaaaagtata agcagggtat
    27481 ttacacaggt tgttgatgaa aaacatcgta ttatggccgc tgcatttcgg gagctacttt
    27541 ctgaaataga agagctaagg acaattattg actttggtga atacaaaccg ggggagaatg
    27601 cctctcagga taaaatctac aacaaaatat ctgttgttga gagttttctg aagcaagatt
    27661 atcgactcgg ttttacttat gagcagacaa tggagcttat tggtgaaaca attagataag
    27721 gttttaaaaa tcaaagataa atatcaacgg tctgttaaat taattgaagc acacatatta
    27781 acactaaaaa aaaaaactct ttgtatcgag atgtggaggc attagataaa aggattatat
    27841 acttcctgca actggagaat gacttagaac ctgtaggcgc tcagagtgtt agtcagcttt
    27901 tcaatacaag acgtaagatc gctatagtaa aaaagcatat tatccaatat cagtctgaac
    27961 gaattcttct aaagggaaga attgaagaaa ttcaaaaaga tattgatgag gcaaatgctt
    28021 ctaaaagaaa actgcttcat aaagagagta aaatatgtaa acggattggc ttgattaaaa
    28081 gaaataactt tgccaaacag ttgatacttg atgagttatc acaagaggat atgaagtatg
    28141 gcattagaca atataaacct aaattttagt agtgacaaac agatagaaaa atgtgagaaa
    28201 ctatcttcaa tagataatat tgatagtctc gttttgaaga agaagaggaa ggttgaaatt
    28261 cctgagtact ctttaattgc atctaactat tttactattg ataagcactt tgaacataag
    28321 catgataaag gagaaattta tagtggcatt aaaaatgcgt tcgaacttag aaacgaacga
    28381 gcgacatatt ctgatattcc agaatcaatg gccattaaag aaaatatttt gataccagat
    28441 caagatatca aagcaaggga aaaaataaat atcggcgata tgagggggct cttttcatat
    28501 aataagagtg gaaatgcaga caagaacttc gaaagaagtc atacttcttc tgtaaaccct
    28561 gataatctgc tagaatctga taatagaaat ggtcaaattg gtttaaaaaa tcatagcttg
    28621 tctattgata agaatattgc tgacatcatt tctttactaa atggaagtgt tgctaaatca
    28681 tttgagctgc ctgtaatgaa taaaaatact gcagacataa ccccatccat gtcattgcaa
    28741 gaaaaatcaa tagttgaaaa tgataaaaat gtttttcaaa aaaatagtga aatgacttac
    28801 cactttaaac agtggggggc tggacattct gttagtattt cagtggagtc tggttctttt
    28861 gttctaaaac cgtcagatca atttgtagga aataaacttg acttaatttt gaaacaagat
    28921 gctgagggta attacagatt tgatagcagt caacataata aggggaataa aaataatagt
    28981 acaggatata atgaacagag tgaagaagaa tgctaagaat taaacatttt gacgctaacg
    29041 aaaaactaca gattttatat gcaaagcaac tctgcgagcg tttttcgatt cagacattca
    29101 aaaataaatt tacaggcagt gaaagtttag tcactcttac ttccgtgtgt ggggattggg
    29161 taattcgtat tgatacatta tcttttttga aaaaaaaata cgaggtattt tcaggatttt
    29221 ctacacaaga atctttactg catttatcaa aatgtgtctt tatagagtcg tcatctgtat
    29281 tttcgattcc agaactgtct gataagatta ctttccggat cacgaatgaa atccagtatg
    29341 caactactgg gagtcattta tgctgttttt cctcttcttt aggtattatt tattttgaca
    29401 agatgccggt attacgtaat caagtttctc ttgactcatt gcatcatctt ttagagtttt
    29461 gcttaggttc atctaatgta aggctggcta ctttaaaaag aattcgcact ggtgatataa
    29521 tcatagttca gaaactttat aatttattat tgtgtaatca agttattatt ggggattata
    29581 ttgtgaatga taataatgag gcaaaaatta atctgtcaga aagtaatggt gagtcagaac
    29641 acacagaagt ttctttggca ttattcaatt atgatgatat caatgtaaaa gtggacttta
    29701 ttcttttaga aaaaaatatg acaatcaatg aactaaaaat gtatgtagaa aacgaattat
    29761 tcaagtttcc cgatgacata gttaaacatg taaatattaa agtaaatggt tctttggttg
    29821 ggcatgggga acttgtttct attgaggatg gttatggtat cgagattagt tcttggatgg
    29881 taaaggagta atctcatgct gagtgacatg tccctcatcg ctacactttc tttttttacg
    29941 ctattaccat ttttagttgc tgctggtacc tgttatataa agttttctat tgtttttgta
    30001 atggtacgaa atgcacttgg tttgcagcag gttccatcaa atatgactct caatggtatt
    30061 gcattgatta tggctttgtt cgtgatgaaa cccattattg aagctggtta tgaaaattat
    30121 ctaaatggcc ctcaaaaatt tgatacaata tctgatattg tccgtttttc tgattctggc
    30181 ttgatggaat ataagcaata cttgaaaaag catacagatc ttgagttagc acgttttttc
    30241 caaaggtctg aagaagaaaa tgctgattta aagagtgcag agaataatga ttattcattg
    30301 ttttccctct tgcctgcata tgcgctaagt gaaatcaaag atgcgttcaa aataggtttt
    30361 tatttatatt tgccatttgt tgttgttgat cttgttattt caagcatttt acttgcactt
    30421 ggtatgatga tgatgagtcc aatcacaata tcagtaccaa taaaacttgt attatttgtt
    30481 gcgcttgatg ggtggggtat cctgtctaag gccttgattg aacaatatat caatattcct
    30541 gcttagatga tggaggtaat gtgtctgata tagtttatat gggtaataag gctctttatt
    30601 taatccttat cttttcctta tggccagtag gtatagctac ggttattgga ttatctattg
    30661 gtttgttaca gacagtgact caactccaag agcagacact tccttttggt ataaagctta
    30721 taggtgtctc aatatctttg ctacttcttt ctggatggta tggtgaggtt ttattgtctt
    30781 tttgtcatga aataatgttt ttaattaaga gtggggtttg atggacattt caagctggtt
    30841 cgaaagtatt catgtgtttt taatactcct gaacggcgtt ttttttagat tagctccatt
    30901 atttttcttt cttccatttt taaataacgg tataatttct ccatctatta gaatacctgt
    30961 gatttttctt gttgcatcag gattaattac ttctggtaag gtagacatag gttcttctgt
    31021 ttttgaacat gtttatttcc ttatgttcaa ggaaataatt gttggccttc ttctctcttt
    31081 ttgcttgtct cttccctttt ggatatttca tgctgttggt agcattattg acaaccagcg
    31141 tggggcaacg cttagtagtt caattgatcc tgccaatggt gttgatacgt ctgagttggc
    31201 aaaatttttc aatctttttt ctgcagttgt atttctatac agtggtggta tggtctttat
    31261 tttagaatcc atacaattgt cttataatat atgcccgtta ttttctcaat gttctttccg
    31321 tgtctcaaat atcttaacat ttctgacttt attggcaagt caggctgtta ttttagccag
    31381 tcctgttatg atagtattgt tactatcaga agtattactt ggtgtattat cgagatttgc
    31441 tccgcagatg aatgcttttt ccgtatcatt aactattaaa agtttacttg caatatttat
    31501 tattttcatc tgttcttcta ctatttactt ttctaaagtt caatttttcc tcggcgaaca
    31561 taaatttttc acaaatctat ttgttagata aaatattatg gcaaataaaa cagaaaagcc
    31621 gacacctaaa aaactaaagg atgccgcaaa aaaaggacag tcatttaaat ttaaggattt
    31681 aacgactgtt gttattattc tggtagggac atttactata atatcattct tttccttaag
    31741 tgatgtaatg ctcttataca gatatgtaat aattaatgac ttcgaaatta atgagggtaa
    31801 atactttttt gcagtggtta ttgtcttttt taagataatt ggcttcccac tttttttctg
    31861 tgttctttcg gctgtgttgc caacattggt tcaaacaaag tttgttcttg cgactaaagc
    31921 tatcaagatt gatttttcag tattaaaccc tgttaaaggg ttaaaaaaaa tatttagtat
    31981 aaagacaata aaagaatttt tcaaaagcat tctgcttctt attattctag cattaacaac
    32041 ctatttcttt tggattaatg accgaaaaat aattttttct caggtgtttt ctagtgttga
    32101 tggcttatat cttatttggg ggaggctgtt taaggatata atattatttt tcttggcatt
    32161 ttctattctt gttattattc ttgactttgt gattgagttc attttataca tgaaagatat
    32221 gatgatggat aaacaggaga taaaaagaga atatatagag caagagggac actttgagac
    32281 aaagtcgaga aggcgtgagt tgcatatcga gattctttca gagcagacta aatctgatat
    32341 acgtaattca aaattagtgg taatgaaccc gactcatatt gcaattggta tttattttaa
    32401 tccagaaata gcgcctgcac cttttatttc tctcattgaa actaaccagt gtgccttggc
    32461 tgtcagaaaa tatgcaaatg aagttggtat accgactgtg cgtgatgtga aattagctag
    32521 aaaactatat aaaacacata caaaatatag ttttgttgat tttgaacact tggatgaagt
    32581 cctacgtctt attgtttggc ttgagcaggt tgaaaacact cattaataag taaaggagat
    32641 gtgttatatg ggagttaatt tctgtaataa aataggtatt gatcagtctg aatttgaaat
    32701 agaatcttcc atcattaact ccattgctaa tgaggtattg aacccaatat ctttccttag
    32761 caataaggat ataataaatg ttttactcag aaagatttca tctgaatgtg acctggttag
    32821 aaaagacatt tatcgttgtg ctctggagtt ggtcgttgaa aaaacacctg atgatttata
    32881 atcattcata ataactcttt tgctgatata aatatttttg ttgaagatat ttatgtagtc
    32941 acaggatcaa ttgtagaaaa ttgttatgtt tttgttagga actgcactaa ataatatatg
    33001 ataagacagc aaaaacgact gaccataatc cttcttttat taggagttga taagagagac
    33061 tattcatcct gtaatgttaa aactctgtta tatagtattc gcgattatgc aaagtctgta
    33121 aatgatcatg agatactaac cgagagtaat cgtttattat ctcattgcat ttcagatagt
    33181 aacggagctt tctttaaaag tagtaaatac gttcccctta agtacttacg taaaaggaga
    33241 atagcacgaa agataccaaa tgattagcac tttgctagta agcttgctgg taaactatat
    33301 tgaccgttgg gtttgttttg agactgtaat ttaaattgtg taattgcctg tttttgatat
    33361 gtttactcca acaacggaga caggtaaatt atggacgaaa agaagcttaa agcccttgcg
    33421 gctgaactgg ctaaaggcct caaaactgag gccgacctca atcagttttc ccgtatgctg
    33481 acgaaatatc ccggctctta aaacgatagt ggctcaggct ttcatttttc agcgttcccc
    33541 agaagatttc catcggagcg ttgtcgtaac agttaccttt acgcgacatt gatgttttca
    33601 gaccagactg ctcctgtatg acccggtaat cgtatgcgca gtactgtgaa cctcgagcgg
    33661 agtggtggat tagcccggca ggtggg
//
LOCUS       DAEEGA010000040        33638 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEEGA010000040 DAEEGA010000000
VERSION     DAEEGA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33638
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.040"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="KLH46_002045"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="KLH46_002045"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBE0378735.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="KLH46_002046"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="KLH46_002046"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBE0378736.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="KLH46_002047"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="KLH46_002047"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBE0378737.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="KLH46_002048"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="KLH46_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBE0378738.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="KLH46_002049"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="KLH46_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBE0378739.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="KLH46_002050"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="KLH46_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBE0378740.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="KLH46_002051"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="KLH46_002051"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBE0378741.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="KLH46_002052"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="KLH46_002052"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBE0378742.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="KLH46_002053"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="KLH46_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBE0378743.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="KLH46_002054"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="KLH46_002054"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBE0378744.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="KLH46_002055"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="KLH46_002055"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBE0378745.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="KLH46_002056"
     CDS             8212..9075
                     /locus_tag="KLH46_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBE0378746.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="KLH46_002057"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="KLH46_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBE0378747.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="KLH46_002058"
     CDS             10411..11028
                     /locus_tag="KLH46_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBE0378748.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="KLH46_002059"
                     /pseudo
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="KLH46_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="KLH46_002060"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="KLH46_002060"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBE0378749.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="KLH46_002061"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="KLH46_002061"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBE0378750.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="KLH46_002062"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="KLH46_002062"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBE0378751.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="KLH46_002063"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="KLH46_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBE0378752.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="KLH46_002064"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="KLH46_002064"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBE0378753.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="KLH46_002065"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="KLH46_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBE0378754.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="KLH46_002066"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="KLH46_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBE0378755.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="KLH46_002067"
     CDS             21859..23568
                     /locus_tag="KLH46_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBE0378756.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="KLH46_002068"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="KLH46_002068"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBE0378757.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="KLH46_002069"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="KLH46_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="KLH46_002070"
     tRNA            27625..27719
                     /locus_tag="KLH46_002070"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            28020..29204
                     /locus_tag="KLH46_002071"
     CDS             28020..29204
                     /locus_tag="KLH46_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0378758.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPREAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29616..30659
                     /locus_tag="KLH46_002072"
     CDS             29616..30659
                     /locus_tag="KLH46_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBE0378759.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPENNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31174..31602
                     /locus_tag="KLH46_002073"
                     /pseudo
     CDS             31174..31602
                     /locus_tag="KLH46_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31632..32783)
                     /locus_tag="KLH46_002074"
                     /pseudo
     CDS             complement(31632..32783)
                     /locus_tag="KLH46_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32848..33502
                     /locus_tag="KLH46_002075"
                     /pseudo
     CDS             32848..33502
                     /locus_tag="KLH46_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8422 a         8834 c         8291 g         8091 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcatgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatggttt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccatcac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtgctgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cggatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtagatg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaaagccg gaggatgtca
    28021 tggcattaac cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg
    28081 ctgatggcgg cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga
    28141 agtaccgctt tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc
    28201 tagccgatgc tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc
    28261 caagggaggc aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg
    28321 aggctattgc tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg
    28381 atgaaatcat tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg
    28441 ccgaaatcaa accaatgaag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc
    28501 tggagaaaat gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta
    28561 ctggccgggc agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa
    28621 aagtacattt tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg
    28681 gttataccgg aagtataatc actaagacag ctactcagat cattatgtta accggcgtac
    28741 gaacacaaga actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg
    28801 agatcccggc agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg
    28861 tcattatgct atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg
    28921 gtagaaatga tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac
    28981 ttggatataa gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc
    29041 atgaacaagg ctttaattcc gcctggattg aaatgcagtt agctcatgtg gataaaaatt
    29101 ccatcagggg tacctataat catgccctgt atctcgatgg ccgccgtgaa atgatgcaat
    29161 ggtacgctga ttacattgat tcactctcca gccgggaaag ttaagtcaga aatacttttc
    29221 cctgatgttt tcctcaatta agtattttat cctctggcct acaaggctgg aggatttttg
    29281 ttattatatt atttttttgc gtcttcaaac ttattacaaa aacataatta aaaacaaatt
    29341 gacattttta aattggcaat tacaaaatac agccttgcag ttatttcgat acctttagca
    29401 aaggaatagt tgattcagat ataatttagc atttcaatca tctgacatca ttataaaaaa
    29461 tgagattcac ataaagataa aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag
    29521 caatattcat tcggtgccaa gacaggactt gatacacaga atctcaactt ttatgctctc
    29581 agactatatc accacatgtt ttacaggctc agaaaatgaa cgatagatta tgctttgaag
    29641 tgcacgataa caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg
    29701 agtttgaaga agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca
    29761 acaaactccg acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg
    29821 catatgcatt tctggaacag aatgccccgc gtaaggcact gaacgccgct cttaaaggac
    29881 tggcggcagg taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc
    29941 ccgaaaaccg gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac
    30001 agcgccatca ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaaaata
    30061 atcagggggc tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag
    30121 ccttcagtgt actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc
    30181 tcctgcattt tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg
    30241 ccacaaacac ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg
    30301 tgtggcatga cttctccggc tctctggata cggcagaaga ttactatgca acatactcac
    30361 cattatgggg acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct
    30421 ccgtgctgca tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg
    30481 atttcgaaat atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca
    30541 agacactttc tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc
    30601 cctggatatt gcccttttcc gcagcaggga tgaaacattc aagcatccag tatcagtaga
    30661 tattgcatta gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt
    30721 tccttcccca ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag
    30781 cgttgtaaat tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt
    30841 tatcgtcaaa cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa
    30901 tcccctcctc tggcgttttc ggttgaactg gtatccccgt ccaacacagg cagccattat
    30961 aaacagtaaa aaattgtatt cgctgttatg aaatcaatag tcctgacgta ctgttgaatg
    31021 tgcttgtgtt ctgaacctcg tacaaccagt acatttgata tcgaattata aacaaaacat
    31081 tgcatccaac ttttttaata tggacaatag ttgtccatac ggtggtttta tattcgataa
    31141 atcaatggat ttcattgagt cacaggactg gtaatggatg aaaatgcttt aaggtttgcc
    31201 tcatactggc gcaactcgct tgcagatgct gagtcaggaa agggctgttt tgaacggaaa
    31261 gacgcccaaa atttcactca ctggcatggg atagcggcgg gacgtcttga cgaagcgatt
    31321 gtcaataaat tttttaaggg agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc
    31381 ccaaaagttt atttccggtt actgcagcat ggtaaggacc gttccgcagg cacgcctgat
    31441 attgttacac cgatagtgac gccagccttg ctaagccgtg agggtttttt atatccgacg
    31501 ccagcgacct ccattcccag agacctgctt gaacctttgc caaaaggagc attttcgatt
    31561 ggtgagattg ggcagtatga caaatacaag acgacccata ctgatcctac ccacgtaatg
    31621 tggacacggc cctaagcgag attatggttt tcaaattgtt ccgggctgag accgccacag
    31681 gcactgtgac gacgccagcg attgtaatca cactcgatat aattaaacac cgttgcccgc
    31741 attatttccc ggctgctaaa gcgttccccg tggatacatt ccaccttcag cgaatgaaag
    31801 aagctttcca cacaggcatt gtcataacag tagcctttcg cactcatact gccacgcagg
    31861 ttgtgtcgct tcagcattgc ctgataatcc cctgaacagt attgaccacc acggtccgta
    31921 tgaacaatga cgctttccgg gcgtcttctc cgccacaacg ccatttgcag tgcatcacag
    31981 gccagttgtg ctgtcattcg cggtgacatc gaccagccaa tgacggagcg tgaccacagg
    32041 tcgatgacta ctgcgagata caaccagccc tcatcggtac gcaagtacgt gatgtcaccc
    32101 gcccacttct ggtttcgggc cactggcgct gaagtcctgc tccagcagat tctccaatac
    32161 gggcaggcca tgtgcacggt agctgaccgg gctgaacttc cggctggctt tcgcccgcag
    32221 cccctgacga cgcaggctgg cggcaatggt tttaatattg aactccggca gttcgtcagc
    32281 aaggcgggga gcaccgtatc gctgctttgc ctcaaagaat gccttatgga cagcggcatc
    32341 gcaggtgagc cgaaactgtt ggcgcaggct catctggtga cgacgcctga gccagacata
    32401 ccagccgctg cgggcaactc gaagtacacg acacatcgct ttgatgctga actctgcccg
    32461 atgattttcg ataaagacat acttcatttc aggcgcttcg cgaagtatgt cgcggccttt
    32521 tggaggatgg acagttcctc agcctgctcc gccagttgtc gtttaaggcg gacattttca
    32581 gcggccagtt cgctttcgcg ctctgacgaa ctcatttgtt gctgctgttt actgcgccag
    32641 gcataaagct gagattcata caggctgagt tcacgggctg cggcggccac accgatgcgt
    32701 tcagcgagtt tcagggcttc gttacgaaat tcaggcgtat gttgtttacg gggcttcttg
    32761 ctgattgata ctggttttgt catgagtcac ctctggttga gagtttactc acttagtcct
    32821 gtgtccacta ttggtgggta agatcaccct ccattaaaac taaaacgggc gctttctgat
    32881 atatcatctg taagcgcagt aataccatta gtagcacctc tcggcggcgc attgattatt
    32941 gaatattata actggaatgt tatatttctt gttatgatgc tgttcagtat tataataatg
    33001 ttattatcac caaagataac tcatgcaaat gagaaagtaa tatgtcaaaa cagtgcatcc
    33061 tttgtgacca aagagtttat ctgcggcact atacttgtct cattaatact cagttctcta
    33121 ttttgctaca tatcaatatc accatcaatt ttcatgcata cttatggcct gagtactttt
    33181 agttactcaa ttattttctc cttctcagtt ttatttttca taatcggaaa tcaattatca
    33241 aagattgaaa aattatccaa cctatggttt ttattaccca atagccaatt ttatcgcatt
    33301 acaaataaat ggttcaaaaa caggatttgc cgcatctatt acaggtttta ctcaaacaat
    33361 aagcgctggt acaatcagtt tcctgtcagt aaccgtaagc gacaacggtg ttggtcccgg
    33421 aactacttta ggtcttggtt cactaataac ttcattactg tcaatagccg taatcgccat
    33481 tgcaggaaat aataaaaagt aagtgtactc tgactgcgta tgtatctact tgaaccgccc
    33541 cggttttcct ggagagtgtt ttatctgtga actcaggctg ccagatcatc gtttccgatg
    33601 gaagcataat aagctttttc tgcttctgcc ggagggat
//
LOCUS       DAEEGA010000041        32634 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEEGA010000041 DAEEGA010000000
VERSION     DAEEGA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32634
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.041"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..956)
                     /locus_tag="KLH46_002076"
     CDS             complement(<1..956)
                     /locus_tag="KLH46_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBE0378760.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="KLH46_002077"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="KLH46_002077"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HBE0378761.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="KLH46_002078"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="KLH46_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="KLH46_002079"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="KLH46_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBE0378762.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="KLH46_002080"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="KLH46_002080"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBE0378763.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="KLH46_002081"
     CDS             complement(5018..6346)
                     /locus_tag="KLH46_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBE0378764.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLFGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8702)
                     /locus_tag="KLH46_002082"
     CDS             complement(6354..8702)
                     /locus_tag="KLH46_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBE0378765.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="KLH46_002083"
     CDS             complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="KLH46_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBE0378766.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="KLH46_002084"
     CDS             complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="KLH46_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBE0378767.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="KLH46_002085"
     CDS             complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="KLH46_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBE0378768.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="KLH46_002086"
     CDS             complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="KLH46_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBE0378769.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="KLH46_002087"
     CDS             complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="KLH46_002087"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBE0378770.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15317..16552
                     /gene="moeA"
                     /locus_tag="KLH46_002088"
     CDS             15317..16552
                     /gene="moeA"
                     /locus_tag="KLH46_002088"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBE0378771.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16552..17301
                     /gene="moeB"
                     /locus_tag="KLH46_002089"
     CDS             16552..17301
                     /gene="moeB"
                     /locus_tag="KLH46_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBE0378772.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="KLH46_002090"
     CDS             complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="KLH46_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBE0378773.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18273..19172
                     /locus_tag="KLH46_002091"
     CDS             18273..19172
                     /locus_tag="KLH46_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBE0378774.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19178..21610
                     /locus_tag="KLH46_002092"
     CDS             19178..21610
                     /locus_tag="KLH46_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBE0378775.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21756..22571
                     /gene="ybiV"
                     /locus_tag="KLH46_002093"
     CDS             21756..22571
                     /gene="ybiV"
                     /locus_tag="KLH46_002093"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBE0378776.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22723..23988
                     /locus_tag="KLH46_002094"
     CDS             22723..23988
                     /locus_tag="KLH46_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBE0378777.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24229..25821)
                     /locus_tag="KLH46_002095"
     CDS             complement(24229..25821)
                     /locus_tag="KLH46_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBE0378778.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26040..26960
                     /gene="ldtB"
                     /locus_tag="KLH46_002096"
     CDS             26040..26960
                     /gene="ldtB"
                     /locus_tag="KLH46_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBE0378779.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27018..28136)
                     /locus_tag="KLH46_002097"
     CDS             complement(27018..28136)
                     /locus_tag="KLH46_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBE0378780.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="KLH46_002098"
     CDS             complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="KLH46_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBE0378781.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28786..28914
                     /gene="mntS"
                     /locus_tag="KLH46_002099"
     CDS             28786..28914
                     /gene="mntS"
                     /locus_tag="KLH46_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBE0378782.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29186..30769
                     /gene="opgE"
                     /locus_tag="KLH46_002100"
     CDS             29186..30769
                     /gene="opgE"
                     /locus_tag="KLH46_002100"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBE0378783.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="KLH46_002101"
     CDS             complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="KLH46_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBE0378784.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31385..31450)
                     /locus_tag="KLH46_002102"
     CDS             complement(31385..31450)
                     /locus_tag="KLH46_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378785.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31685..32572
                     /gene="rhtA"
                     /locus_tag="KLH46_002103"
     CDS             31685..32572
                     /gene="rhtA"
                     /locus_tag="KLH46_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBE0378786.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         8168 a         8701 c         7883 g         7882 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattactgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaaaca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttcacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc
     8701 atgttaatac atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct
     8761 tgctgcggct ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact
     8821 tgcttagggc aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac
     8881 tatcctttaa ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc
     8941 agtacggtca gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata
     9001 tccgctcgtg cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg
     9061 ccgagaaatg acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac
     9121 accacgatag aagagacggt cccaggcagg atatgacgca ataaaatggt catatcgctg
     9181 gcaccaatac tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg
     9241 cgcaccaggc gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca
     9301 atgccgcttc ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac
     9361 agcacatcgc aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac
     9421 aagcccagca acgtccctat cgccacaccg ataaacacgg caaacacgcc cgccgccagc
     9481 gagatttgcg caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca
     9541 aaccagtgct gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg
     9601 tcatagggag cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat
     9661 gcggcggtca tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta
     9721 cgtacctggt caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta
     9781 aatagtcgca tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca
     9841 ccactaagtt gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg
     9901 ggtaatcgcg catttctaca gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa
     9961 ctttctccac cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca
    10021 ctaccgggat catcgcgttg cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc
    10081 ctttcgccct cgcggtacgc atataatctt cgcttaaaac gtcgacaaat gacgcacggg
    10141 taaagcgcgc catcacggcg gccactgccg cgccgagcgt cagggagggt aaaatgtagt
    10201 gctgccaact gtctgctccc acagtaggca gccagcccag ttcaacggag aatacctgaa
    10261 ttaaaaacat ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac
    10321 tcaatcgatc cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa
    10381 atatcaccgc ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg
    10441 caatctcatc ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag
    10501 cattgctgat atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac
    10561 gcaccagttc tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc
    10621 cgggcagcat gtggacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca
    10681 accccagtaa tcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa
    10741 atccgcgtct tcaaagctga agccggtatc tggcatgatc caaaaaccgg tcaggttttt
    10801 actgtgtgcc gacaccagtt tttctaccac cagcgggatc cacggcgatt cttgccagat
    10861 gatatcctgc gccgccttat ataagcgggt cttttccgcc ggatcattcg ttttcagtgc
    10921 ctgagccagg aagtcatcca cctgtttatt gctgtaaaac gcggtattaa acagcgtcgg
    10981 tggccagttt tgcgaggcaa acagcggcga tagtgcccag tcggcttcgc cggttgaagc
    11041 cgaccagcca gtgtagaaca tccgcacgcc actctctttt tgccctttac cttcaacttc
    11101 tgccgcccgc tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa
    11161 ctgctgctgg gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag
    11221 cgtggtactg aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg
    11281 atcgtaaggc cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt
    11341 agctggcgtt gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta
    11401 attcagcgcc tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat
    11461 ataacgctgc ataattgacg gactggccat caactcgata tttttgtttt tctccagcag
    11521 tgtggcctgc tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc
    11581 cgcgcgggtg ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc
    11641 tggctgccag taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc
    11701 cagttcatac ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag
    11761 cgctgtcggt gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa
    11821 cggctgtttg agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt
    11881 cttatacagg ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc
    11941 ttttaccgcc actgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt
    12001 cacggtgtaa gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag
    12061 tttcatctct ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga
    12121 taacgtgtca ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac
    12181 cacatctttg gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac
    12241 taacccacta cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca
    12301 tgaatgcgta ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg
    12361 agacggctgc cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca
    12421 gtacacgctg cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac
    12481 gcgtataagg atgctgcggg ttttcgaaga ccgcgcaccg tgaaccaatt tcaacaattt
    12541 gcccgagata catcaccgcc acacgatgac taatccgctc taccacagcc atatcgtggg
    12601 agataaacag atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct
    12661 gcccgcgaat agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat
    12721 tcaatgccaa cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg
    12781 ggtaacgcca ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg
    12841 cagccgcgtc tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac
    12901 cgatggtctg acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc
    12961 ggcgcaatgc ctgaagtttg ccaggtgaca aggtatcgat tcgctgaccg ttaaagataa
    13021 tttcgccgcc ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc
    13081 tgccagactc acccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct
    13141 caacggcatg cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg
    13201 tgacaagatt acgcacccgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg
    13261 ggggttcctg tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta
    13321 accctttcat cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt
    13381 gcggtgcatg aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca
    13441 ccagtacccg atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc
    13501 ccatcgacat ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga
    13561 catccagcgc ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca
    13621 tcgcaatcat cactcgctgg cgcatcccgc cagagagttg atgcgaataa cgtgaaagaa
    13681 tggtttgtgc ctccggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt
    13741 cttcacgact ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa
    13801 atactgggtt cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgca
    13861 catggcgcat ttgtgcagcg ctctgctcgc taagttcaat cacatcacga ctgcgccgcc
    13921 gcaacagcat tttatcgcac tgtactaaac cgcccgcctg ttccaacaga cgcatcaatg
    13981 ccagcgcagt cactgactta ccggagccgg attcgcctac aattgccagc gtctcaccgc
    14041 gttgcagact aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg
    14101 caatattaag attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca
    14161 acggtgtccc ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag
    14221 gcgcgataca ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg
    14281 attaagccac cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct
    14341 tccgcgagac ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg
    14401 cggatgaaga cttcgcctgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag
    14461 catccggcac ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg
    14521 cctgtggacg tggctgccgc cagattgccg tctaaatcca acgccacggc ccccacggtg
    14581 cccattttct gtttttcatc cagtggcgca ccgctatggt cgaggactgt tgccccttcc
    14641 tcgcgcgctg ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggtgagacg
    14701 cgctccatgc catgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc
    14761 gggctttgct ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc
    14821 gccaccgcac cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt
    14881 tcatcacgcg taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga
    14941 cgcaccgctt ccgtcaccac atccagcgca ctttcgccgg cttccagcat tttctgcccg
    15001 gtttcaacaa tggcagacaa cgcctcgatg tagcgtaatt cctgttgcag actcatctgc
    15061 gcgcggctaa ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc
    15121 gtccattaag ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca
    15181 ttcaattttt gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc
    15241 ccgaggtggc atacagtcta tggcgttttt ctgacataat aggcaaattc gattttgcct
    15301 ccgcaggagt gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa
    15361 tgagatgctt tctcgcgtta ccccactgac cacccaggaa acgctgccac tggttcagtg
    15421 ttttggtcgt attctggcga gcgatgtcgt ttcgccactg gatgtcccgg ggtttgataa
    15481 ctccgctatg gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc
    15541 cgttgccggt aaatcctttg ccggtcagcc ataccatggt gaatggcctg cgggtacctg
    15601 cattcgtatt atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga
    15661 gcagactgaa caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa
    15721 tattcgccgt cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg
    15781 cctgactacc gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat
    15841 tcgtaaagtg cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc
    15901 gctgggcgac ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca
    15961 gctgggttgc gaggtaatca acttagggat tatccgcgac gatccacatg ccctgcgcgc
    16021 cgcatttatt gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt
    16081 gggtgaagcg gattacacca aaatgattct tgaagagctg ggggagatcg ccttctggaa
    16141 gctggcgatt aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg
    16201 cggcctgccg ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt
    16261 gctggcaaaa ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac
    16321 agcgtcccgc ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg
    16381 caacgccgac ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag
    16441 ctcctttagt ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt
    16501 gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa
    16561 ctcagcgatc aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt
    16621 gacggtcagg aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc
    16681 tgcgcagctt cgcagtatct ggcaagcgcc ggtgtcggca acctgacact gctcgacttc
    16741 gacacggttt cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg
    16801 caaccgaagg tggaatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt
    16861 acgccagtca atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat
    16921 ctggtgctcg actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt
    16981 gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc
    17041 ttcacttatc aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac
    17101 gcgttaacct gcgtagaagc aggcgtgatg gcaccgttga taggcgtaat tggttcgttg
    17161 caggcaatgg aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc
    17221 gtcatgtacg atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg
    17281 tgtgaggtgt gcgggcagta attgccacct ccaagcctga taagacgcga caagcgtcgc
    17341 atcaggcatt tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg
    17401 ggcacagatt tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat
    17461 gaggatgcag aacgtccggt taaatcgacg ttctgccaaa cgctccctgc cagtcctgct
    17521 caaacttcgt cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca
    17581 gtggcagagt aattgattca catccggcca gtaagcagtc cagcgcctga cgcggggttt
    17641 tgaaactcgc tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt
    17701 cggtcacagt ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa
    17761 catattccgc acctgccagc gccgacagca gcccttgtgc cgcgccatat accgcggttc
    17821 ccagcgtcgg aatcccttcc gcttttaaca tcttaatcgc tgccagcccc tcggctgtca
    17881 ccggaacttt caccacgata tccgcaataa tagaacgcag cttaagcgcg tcattcacca
    17941 tcccttcagc agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt
    18001 catgaagttg cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt
    18061 tagtggtcac acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg
    18121 aagtatccag atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac
    18181 tatcacggga aaactctctg aacagttgac gcacatcaat ctgactttca ttcgaaagta
    18241 ttttaatgct tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga
    18301 cccatgatgg ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc
    18361 gctggtgtca gaacccggaa agccgcgccc gtacgcagga tctgctgtat gacgcacgac
    18421 tgtgtctgga aggctgcgag ctgtgcgcta aggccgcgcc ggaagtgatt gagcgcgcgc
    18481 tgaatggttt gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg
    18541 actgctgtcc gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca
    18601 tggcgaccgt tctgcgcgat aaaccgtttt acgatcgcag cggcggcggc ttaacgcttt
    18661 cgggtggtga acccttcatg cagccggaaa tggcgacggc gctgctgcaa gcctgccacg
    18721 aggcaggcat tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc
    18781 cttctctgcc ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt
    18841 ttaaacagtg gaccgacggt aacgccgcca gggtgctgga taacctgaaa aaactcgccg
    18901 cagcgggcaa aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa
    18961 cctctgtaaa agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt
    19021 ttctgcccta ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg
    19081 ccccggaaaa accgcttgat gcgccagagc tgctcgattt tgcccagcag tatgcctgcc
    19141 agaaagggtt aaccgcgacc ttacgaggat aacaaccatg accacactga aactggacac
    19201 gctcagcgac cgcattaaag cgcacaaaaa tgcgctggtg catattgtga agccgccagt
    19261 ctgtaccgag cgcgcgcagc actataccga gatgtatcaa caacatctcg ataagccgat
    19321 cccggtacgt cgcgcgctgg cactggcgca tcacctggcg aatcgcacca tctggatcaa
    19381 acacgatgag ttgatcattg gcaaccaggc aagcgaagtt cgcgccgcgc cgatcttccc
    19441 ggaatatact gtctcgtgga tcgaaaaaga gattgatgat ctggcagatc gtcccggtgc
    19501 tggctttgcg gtgagcgaag agaacaaacg tgttctgcat gaagtgtgcc catggtggcg
    19561 cggtcagacg gtacaggatc gctgctatgg catgtttacc gatgagcaaa aaggtctgct
    19621 ggcgaccggc atcattaaag cggaaggcaa tatgacctcc ggcgatgcgc acctggcggt
    19681 caatttcccg ctgctgctgg aaaaagggct tgatggtctg cgtgagaaag tagcggaacg
    19741 tcgctcgcgc atcaatctga cggtgctgga agatttgcac ggcgaacagt tcctgaaagc
    19801 gattgatatc gtgctggtgg cagtcagtga acacattgaa cgtttcgctg ccctggcgcg
    19861 tgcaatggcc gcgaccgaaa cccgcgaaag ccgtcgcgat gaactgctgg cgatggcaga
    19921 aaactgcgat cttatcgccc accagccgcc gcagactttc tggcaggcgc tgcaactgtg
    19981 ttacttcatc cagttgattt tgcagattga atctaacggt cactcggtgt cgtttggtcg
    20041 tatggatcag tatctctacc cgtactatcg ccgcgacgtt gaactgaacc agacgctgga
    20101 tcgcgaacac gccatcgaat tgctgcatag ctgctggttg aaattgctgg aagtgaacaa
    20161 aatccgctcc ggctcccact caaaagcctc tgcgggaagt ccgctgtatc agaacgtcac
    20221 cattggcgga caaaatctgg ttgatggtca accaatggac gcggtgaatc cactctctta
    20281 cgcgatcctc gaatcctgcg gtcgcctgcg ttccactcag cctaacctca gcgtgcgtta
    20341 ccatgcagga atgagtaacg atttcctcga cgcctgcgta catgtgatcc gctgcggctt
    20401 cgggatgccg gcattcaaca acgacgaaat cgtgatcccg gaatttatta aactcggtat
    20461 tgaaccgcag gacgcttatg actacgcagc gattggttgt atagaaaccg ccgtcggtgg
    20521 caaatggggc tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg tgatgctggc
    20581 ggcgctggaa ggcgggcgtg atgccaccag cggcaaagtg ttcctgccac aagaaaaagc
    20641 gttgtcggca ggtaacttca acaacttcga tgaagtgatg gacgcgtggg atacgcaaat
    20701 ccgttactac acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca tgctggaaga
    20761 gaacgtgcac gatattctct gctcggcgct ggtggatgac tgtattgagc gagcgaaaag
    20821 tatcaagcaa ggcggcgcga aatatgactg ggtttctggc ctgcaggtcg gtattgccaa
    20881 cctcggcaac agcctggcgg cagtgaagaa actggtgttt gaacagggcg cgattggtca
    20941 gcaacagctg gctgccgcac tggcggatga cttcgatggc ctgactcatg agcaactgcg
    21001 tcagcggctg attaacggtg cgccgaagta cggcaacgac gatgataccg tcgatacgct
    21061 gctggctcgc gcttatcaga cctatatcga cgaactgaaa cagtaccata acccacgcta
    21121 cggtcgtggt ccggttggcg gcaactatta cgcgggtacg tcgtctatct ccgctaacgt
    21181 accgtttggc gcgcaaacta tggcaacgcc ggacgggcgt aaagcccaca ccccgctggc
    21241 agaaggcgct agtccggcct ctggtactga ccatctcggc cctactgcgg tcattggctc
    21301 agtgggtaaa ctgcctacgg cagcgattct cggcggcgtg ttgctcaacc agaaactgaa
    21361 tccggcaacg ctggagaacg aatctgacaa gcagaaactg atgatcctgc tgcgtacctt
    21421 ctttgaagtg cataaaggct ggcatattca gtacaacatc gtttcccgcg aaacgctgct
    21481 ggaggcgaaa aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg cgggttattc
    21541 cgccttcttc accgcgctct ctccagacgc tcaggacgat atcatcgccc gtactgaaca
    21601 tatgctgtaa tctcttcggc ccggctgcgg cgtcgccggg ctaaatcatt cacatcatca
    21661 atttcatcct tactttcatt cgaaatataa tttgtgctct gcgtcacatt gttattacac
    21721 tgttacggtt tcattttcga gccaggagca cacctatgag cgtaaaagtt atcgtcacag
    21781 atatggacgg tacttttctt aacgacgcca aaacgtacaa ccaaccacgt tttatggcgc
    21841 aatatcagga actgaaaaag cgcggcatta agttcgttgt cgccagcggt aatcagtatt
    21901 accagcttat ttcgttcttt cctgagctaa aggatgagat ctcttttgtc gcggaaaacg
    21961 gcgcactggt ttacgaacat ggcaagcaac tgttccacgg cgaactgacc cgacatgaat
    22021 cgcggattgt tattggcgag ttgctaaaag ataagcaact caattttgtc gcctgcggtc
    22081 tgcaaagtgc atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg atggcaaaac
    22141 actaccatcg cctgaaacct gtaaaagatt atcaggagat tgacgacgta ctgttcaagt
    22201 tttcgctcaa cctgccggat gaacaaatcc cgttagtgat cgacaaacta cacatagcgc
    22261 tggatggcat tatgaaaccc gtcaccagtg gttttggctt tatcgacctg attattcccg
    22321 gtctacataa agcaaacggt atttcgcggt tactgaaacg ctgggatctg tcaccgcaaa
    22381 atgtggtagc gattggcgac agcggtaacg atgcggagat gctgaaaatg gcgcgttatt
    22441 cctttgcgat gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac gctaccgatg
    22501 ataataatca tgaaggcgcg ctgaatgtga ttcaggctgt gctggataac acatcccctt
    22561 ttaacagctg acctctctac cggagccatg cgctccggtt ttcccttctt tttacatcct
    22621 gtacatcaca tcaaatcatt aaacttgcat taacttattt ataacttgta ttgtcacatg
    22681 caaattagca tagtttcgaa tgaaagttaa tgcgaggtgg ttatggcttc tacttttacc
    22741 agcgacacat tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg
    22801 gcgcagcttg gcgatgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga
    22861 gtggctgaaa tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat
    22921 gccgatatca aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt
    22981 tgtgcggtga taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg
    23041 ctggactatc tgaaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc
    23101 ttcgggacgc tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa
    23161 atgcaggccc gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg
    23221 acatttgaaa gtgatggaaa gcaatggttt aacccggatg tgagcgtcat ctaccctgac
    23281 cgtatccgcc gccttccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactcc
    23341 ggggcgctgg aacgctggct gcttccagcg tatcagcacg ttttcgccaa cgtctttaat
    23401 ggcaatctgg cgaaatacga tccctggcat gcggcacatc gtacggaagt tgaagagtac
    23461 acggtggaca acaccaccaa atgttccgtg tttcggacat tccagggctg gactgcgctc
    23521 tcggatatgc tgcctggtca ggggttgctg cacgtcgtgc ccattcctga agccatggcg
    23581 tacgtactgt tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg
    23641 cccggaagag tgttgccggt atcagagcaa tggcatccac tgttaattga ggcgttaacc
    23701 agcattccaa aactcgaggc cggagactcc gtctggtggc actgcgacgt catccattcc
    23761 gttgcccccg ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg
    23821 atgtgcgaga aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca
    23881 tcgccgggcg acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt
    23941 gccgacctca acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaatggaa
    24001 gtgaattgat gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg
    24061 ctttcttaat gcctgatgcg gcgttaacgc cttatccggc ctacaaagtc ctgcagattc
    24121 aatatattac aggtactgcg taggcctgat aaacgtagcg catcaggcta ttttgcattt
    24181 gtcatcagcc gtcgtcaccc cgacagtatc ggggtgacgg ataatcactt actcgatccc
    24241 tttactacgc aggtaatctt cgtaattacc gctaaagtcg atcacgcgtt ccggggtgat
    24301 ttccagaatg cgggtcgcca gggagcttac gaactcacgg tcgtgtgaaa caaagatcag
    24361 cgtgccctga tacagttcca gcgccatgtt cagtgactcg atggattcca tatccaggtg
    24421 gttggtcggt tcgtccatga tcagaatgtt cggcttctgc atcattaact taccaaacag
    24481 catccgccct ttttccccac cggaaagcac tttagctggc tttttgatgt cgtcctggct
    24541 gaacagcaaa cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt cctgcttcca
    24601 ctgactcatc cattcgaaca cggtcagatc attttcaaac tcatattcgt ggtcctgggc
    24661 gtagtaacca atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg gttgcagatc
    24721 gcccaccagc gttttcagca gcgttgattt accgacgccg ttggtaccca gtaccgcaag
    24781 tttttcaccg acctccagca gcaggttgag atttttaaac agtggaccgt tatcaaaccc
    24841 tttggtcaga ccttccactt ccagcgcgtt acggaacagt ttcttatcct gttcaaaacg
    24901 gataaacggg ttctgacggc tggaggcttt cacctcttcc agtttgattt tatcaatctg
    24961 gcgcgcgcgc gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc ggctaacgaa
    25021 agattgcaac tcagcaatct gcgctttctt cttggcgtta tcggccagca gacgttcacg
    25081 cgcctgggtc gccgccgtca tgtactcatc gtagttaccc ggataaacgc gcagctcgcc
    25141 gtaatccaga tccgccatgt gggtacagac catgttaagg aagtgacggt cgtgcgagat
    25201 gatgatcatg gtgctgtcac gctcgttcag cacctgttcc agccagcgaa tggtgtcgat
    25261 gtcgaggttg ttggtcggtt cgtcgagcag gagaatatcc ggctcagcaa acagcgcctg
    25321 cgccagaagc acacgcagct tccagccagg agcaacttca ctcatcgggc cgtagtgctg
    25381 ttccactgga attcccacgc caagcaacag ttcaccggcg cgagcttccg cagagtaacc
    25441 gtccatttcg ccgtatttca cttccagatc ggccacttta tagccgtctt cttcactcat
    25501 ttccggcaaa gcatagatgc ggtcgcgctc ctgcttcact tcccacaact ctttatgccc
    25561 catgatcacc gtatccagca cagtgaactc ttcaaaggca aactgatcct gacgcagctt
    25621 accaatgcgc tcgttgggat cgagggaaac gttacccagc gtcggctcaa ggtcgccgcc
    25681 gagaatcttc ataaaggtgg atttaccact accgttcgcg ccaatcaggc cgtaacggtt
    25741 gccgccgccg aatttgacgg aaatgttttc aaacaacggc ttactgccga actgcatggt
    25801 gacgttactg gaaactaaca caggcgtatc ctgaaaagag atatgacaaa ccgcgcatta
    25861 tgccataagt ctgaaagaag atcctgctcc tcgcttaagg tctacactca agccaaaggc
    25921 aaaaaatgtg atttcgtaca catctgattt cactgtgagc tggaatgaac ttataatgcg
    25981 cttccaatac tctaatattc tcaacccaat ggcctgccag gcacaaaatc tcgcttaaca
    26041 tgaatatgaa attgaaaaca ttattcgcag cggccttcgc tgttgtcggc ttttgcagta
    26101 ccgcctctgc ggtaacttat cctctgccaa ccgacgggag tcgcctggtt ggtcagaatc
    26161 aggtgatcac cattcctgaa ggtaacactc agccgctgga gtattttgcc gcggagtacc
    26221 agatggggct ttccaatatg atggaagcga acccgggtgt ggataccttc ctgccgaaag
    26281 gcggtactgt actgaacatt ccgcagcagc tgatcctgcc ggataccgtt catgaaggca
    26341 tcgtcattaa cagtgcagag atgcgtctgt attactatcc gaaagggacc aacaccgtta
    26401 tcgtgctgcc gatcggcatt ggtcagttag gcaaagatac gcctatcaac tggaccacca
    26461 aagttgagca taagaaagca ggcccgacct ggacgccgac cgccaaaatg cacgcagagt
    26521 accgcgctgc gggcgaaccg ctaccggctg tcgttccggc aggtccggat aacccgatgg
    26581 gtctgtatgc actctacatc ggtcgcctgt atgctatcca tggcaccaac gccaacttcg
    26641 gtatcggcct gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac atcaaattcc
    26701 tgttcgagaa agtaccggtc ggtacccgcg tacagtttat tgatgagccg gtaaaagcga
    26761 ccaccgagcc agacggcagc cgttatattg aagtccataa cccgctgtct accaccgaag
    26821 cccagtttga aggtcaggaa atcgtgccaa ttaccctgac gaagagcgtg cagacagtga
    26881 ccggtcagcc agatgttgac caggttgttc ttgatgaagc gatcaaaaat cgttccggga
    26941 tgccggttcg cctgaattaa tcgtcaaaac gtaaaacaaa ggcggactca taatccgcct
    27001 tttttatttg ccagacctta ccaggccggg atcataacta acaaaacgta tcccaccagc
    27061 gccgcccaca acagcatcgg tattgaatag agatggaaac gccaccagat gcggcgatcg
    27121 ttcgccatac gtagcgcaat caaatttgcc agcgatcctg gtaataaacc aaagcccccc
    27181 acgtttaccg cccatgccag taataaagac ggaggtacat aattgagcag caaaatggta
    27241 ctcggcacgt tactgatcac ctgcgataaa ccgactgccg ttaaccataa cccgggttca
    27301 gataaatgac tcacgttacc caacacgcct tgcaacgctg gaagctgggt cagtaaatgg
    27361 acgtcgataa acatcgccat aaacaccagc agcaacgtcc agtccacact caacaccacg
    27421 cgacgcgcca gcagcacaaa gcccgccgcc acaatcacca gtccccacag ctcttgtttg
    27481 aactccagcg ccgtcagaaa gacgatatac agccccagac aattccacac cagccgcggt
    27541 ttccactccg gtgtttgcac ccccgtatgg tattgcagcg cctttccagg gaaacaacac
    27601 cagcacaaca gcaggagtgt cagcatcatt gcgccagcca tcggtgccat ttgggcaata
    27661 aatccggcaa acgaaagacc agaacgtccc cagataagaa tattttgcgg gttgccaatt
    27721 ggcgtcagta gcgaaccagc gttgactgcc agcgcctcaa aaataatcag ccgattaacc
    27781 gggatctcac acagtctttt tagcgtgata gtcagcggaa caacaataaa cagcgcgaca
    27841 tcgttggtca gaaaggtaga aagcagcgcc gccgccagca ccataaacat cgccagccga
    27901 cgctccgtag caaagcggcg caccattttg cgccccagca catcaaaata accgcttaac
    27961 tccacacctt tggtcagcag catcaggccg cttaaggtga tgatggtgtg ccagtcgata
    28021 gcagcaggcc aggatttcgg tgcaaagggc acgaaaaagc ttaatccgat accaacaaga
    28081 attaataact gaaaaaaacg atcgccttgc agcgtgcgta aaaaaggcag gctcatttgg
    28141 caccgtgttt ctgggtaaac aaacgaaaag cgtcgagcgt ctcttcacta acatggtgct
    28201 ccatgccttc cgcgtcgcga cgggcgattt ccggactgac gcccaacacc aacaagaaat
    28261 tttcgactat ctgatgtcgc tcgcggcttt cctgcgccag cttctctcct tctgccgtta
    28321 aaaacacgcc acgccagggg atcatttcaa tcagccccat ggttgccagc cgcttaagca
    28381 ttttagccac cgtcggttgc gaaactccca gacgagcagc catgtccacc tgacgagctt
    28441 cccccacttc cctgatcaag tcagaaatca gctcaacgta atcatcaata agctcgcgcc
    28501 gatgcgcctc tctgacctgg cggaaccctt caacgtgctc ttccacgttc actaactgcg
    28561 tcactttttt tgctattggc gtacctgcgc gacgactcat tgtgcttcct cattatggtg
    28621 acgcatacat agcgttctca tttcgtgcgc acattgtaaa ccagagttgc gaaggtacaa
    28681 aaaattaacg ttttagcaat agctatataa tatagcctgt gctatatctg tatgtaatgc
    28741 aatcatccct caaggatcga cgggattagc aagtcaggag gtcttatgaa tgagttcaag
    28801 aggtgtatgc gcgtgtttag tcattctccc tttaaagtac ggttaatgct gctctctatg
    28861 ttgtgcgata tggtcaacaa caaaccgcag caagataaac cttcagataa atagcggcgt
    28921 cgcggtacgc cgcttcactc ctgcttttat gcaggcataa cgcgttttgg tctgaaaaaa
    28981 accacttttt gtcggatttg caatcccctt cgcaaaagat ttgttcgtca gtagttgacc
    29041 tgaacggcgg ctcgctctat cttcttgcag ccctgcgtat attgcggctc gcggatgcgg
    29101 acccctttcc cctcttcacg cactcttgca ggtattgacc cttgacgcca gggtaagcac
    29161 atggcgtttg ttacgatagt ggcatatgaa tttaaccctc aaagaatcgc ttgttacccg
    29221 tagccgggta tttagcccgt ggactgcgtt ctacttttta cagtcgctat taattaacct
    29281 cggcttaggt taccccttca gtttgctcta caccgctgcg tttacggcta ttttgctttt
    29341 gctatggcga acattgcctc gcgtacaaaa agttctggtc ggtgtcagtt cgctggtggc
    29401 ggcttgttat ttcccttttg ctcaggccta cggcgcgcct aatttcaata cattgctggc
    29461 attgcactcc accaatatgg aagagtcgac cgaaatcctg acgatttttc cgtggtacag
    29521 ctacctggtc ggcttattta tttttgcgct cggcgtaata gcaatcaggc gaaaaaaaga
    29581 gaatgaaaaa gcgcgctgga ataccttcga cagcctgtgt ctggtattca gtgtggcgac
    29641 attttttgtt gctcccgtgc aaaacctggc ctggggtggc gtatttaaac tgaaagatac
    29701 tggctatccg gtatttcgtt ttgctaagga tgtcatcgtc aataataacg aggtgattga
    29761 agagcaagaa cggatggcaa aactttccgg aatgaaagat acctggacgg tcactgccgt
    29821 taagccgaag tatcagacct atgtggtagt gatcggtgaa agcgcgcgtc gcgatgccct
    29881 cggtgccttt ggcggtcact gggacaatac cccgtttgcc agcagcgtta acggtttgat
    29941 atttgctgac tacattgctg ccagtggctc cacgcagaaa tcgcttggct taacgctcaa
    30001 tcgcgtagtc gatggcaaac cacagtttca ggataacttt gtcaccctgg caaatcgcgc
    30061 gggcttccag acctggtggt tttccaacca gggccaaatc ggcgaatacg atactgctat
    30121 cgccagcatc gccaaacgtg cagatgaagt gtacttcctg aaagaaggta attttgaagc
    30181 agataaaaac acgaaagacg aagcgttact ggatatgacc gctcaagtgc tggcgcaaga
    30241 gcactcgcaa ccgcagctga ttgttctgca tctgatgggc tcgcatccgc aggcctgcga
    30301 caggacacaa ggcaaatacg aaacctttgt gcaatcgaaa gaaacgtcgt gctatctcta
    30361 taccatgacg caaacggacg atttactgcg caagctgtac gatcagttac gcaacagcgg
    30421 cagcagcttc tctctggttt acttttctga ccacggtctg gcctttaaag agcgcggcaa
    30481 agacgtgcaa taccttgccc atgatgataa atatcagcaa aatttccagg tgccttttat
    30541 ggtcatttcc agcggcgata aagcgcatcg ggtgattaaa gcccgccgct cagccaatga
    30601 cttcttaggc tttttctcgc agtggacggg aattaaagcg aaggaaataa atatcaaata
    30661 cccgtttata tctgagaaga aagccgggcc gatatacatc accaacttcc agttacagaa
    30721 ggtggattac aaccatctcg gaaccgatat tttcgacccg aaaccttaaa aacaaaaatc
    30781 cgccccgaga ggcggatttt tatatcacca aagtgattag aagcggtaac caacaccggc
    30841 aatccaagtg cctacgtcaa cgctacgaat acggctctgc tcgtaagaga agtccagagc
    30901 aacgttttcc atcgggttga actgcagacc agcaccgtag gagaaaccgt agtcgctggt
    30961 gtcgtgtttg taggtcgggt attcagtggt ctggaattta ccataaccca cacccactac
    31021 accgtagatg cttgcccagt cgttaatgcg gtaagccgga ccagcagtga tgccgtagta
    31081 ctggtttttg ttgtagtcac cagagcttgc agtacggctt ttctcggtgt aagtgaaaga
    31141 accgatcaca cccagcgggc tgttgtcttc ttcatagcgg tatttcaggt tgaaaccgcc
    31201 cattttgttc atttggccct gagcgtcgct ctgtgcgtaa ccgccagtta cggtagaagt
    31261 cgcagctacg gaagtacctg cggtgaaagc cagaactgcg gccagtgctg aaagacatgc
    31321 aatttttttc ataaccacct caaatgtgat tcaaataagt cctaagtttt aaatatatca
    31381 aaaattaatg ggaaactctt cgcgatttgt gatgtctaac gggccatttc atgtaacaga
    31441 acgtttccat acaccgctat ccatctaaat ttaaatcact ttttcagaga actgcgtaag
    31501 tattacgcat gttttccctg tcattcatcc agattattcc taatcaccag actaatgatt
    31561 ccatcaatcc tggcgcattt tagtcaaaac gggggaaaat tttttcaaca aatgctcaac
    31621 cagcattggg tatatccagt acactccacg ctttacttaa gtctagatat ttgtgggaga
    31681 aaggatgcct ggttcattac gtaaaatgcc ggtctggtta ccaatagtca tattgctcgt
    31741 tgccatggcg tctattcagg gtggagcctc gttagctaag tcactttttc ctctggtgag
    31801 cgcaccgggt gtcactgcgc tgcgtctggc attaggaacg ctgatcctca tcgcgttctt
    31861 taagccatgg cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt tttacggcgt
    31921 ttcgctgggt gggatgaatt atctttttta tctttctatt cagacagtac cgctgggtat
    31981 tgcggtggcg ctggagttca ccggaccact ggcggtggca ctgttctctt ctcgtcgccc
    32041 ggtagatttc gtctgggttg tgctggcggt tcttggtctg tggttcctgc taccgctggg
    32101 gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg ccggggcttg
    32161 ttgggctatt tacattttaa gtgggcaacg cgcaggagcg gaacatggcc ctgcgacggt
    32221 agcaattggt tcgttgattg cagcgttaat tttcgtgcca attggagcgc ttcaggctgg
    32281 tgaagcactc tggcactggt cggttattcc attgggtctg gctgtcgcta ttctctcgac
    32341 cgctctgcct tattcgctgg aaatgattgc cctcacccgt ttgccaacac ggacatttgg
    32401 tacgctgatg agcatggaac cggcgctggc tgccgtttcc gggatgattt tcctcggaga
    32461 aacactgaca cccatacagc tgctggcgct cggcgctatc atcgccgctt caatggggtc
    32521 tacgctgaca gtacgcaaag agagcaaaat aaaagaatta gacattaatt aaatttacat
    32581 ttctgtatgg ttatgcataa ccatacagaa tttctcgcta cttttcctct acac
//
LOCUS       DAEEGA010000042        32211 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEEGA010000042 DAEEGA010000000
VERSION     DAEEGA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32211
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.042"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            64..1024
                     /gene="espL1"
                     /locus_tag="KLH46_002104"
                     /pseudo
     CDS             64..1024
                     /gene="espL1"
                     /locus_tag="KLH46_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1196..1303
                     /locus_tag="KLH46_002105"
     CDS             1196..1303
                     /locus_tag="KLH46_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378787.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1356..2114)
                     /locus_tag="KLH46_002106"
     CDS             complement(1356..2114)
                     /locus_tag="KLH46_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBE0378788.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2401..3330
                     /gene="pfkB"
                     /locus_tag="KLH46_002107"
     CDS             2401..3330
                     /gene="pfkB"
                     /locus_tag="KLH46_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBE0378789.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3431..3721
                     /gene="ghoS"
                     /locus_tag="KLH46_002108"
     CDS             3431..3721
                     /gene="ghoS"
                     /locus_tag="KLH46_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBE0378790.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3827..4687
                     /locus_tag="KLH46_002109"
     CDS             3827..4687
                     /locus_tag="KLH46_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBE0378791.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4728..5264)
                     /locus_tag="KLH46_002110"
     CDS             complement(4728..5264)
                     /locus_tag="KLH46_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBE0378792.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5411..6079
                     /gene="hxpB"
                     /locus_tag="KLH46_002111"
     CDS             5411..6079
                     /gene="hxpB"
                     /locus_tag="KLH46_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBE0378793.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6242..6832
                     /locus_tag="KLH46_002112"
     CDS             6242..6832
                     /locus_tag="KLH46_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBE0378794.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6965..8355
                     /gene="tcyP"
                     /locus_tag="KLH46_002113"
                     /pseudo
     CDS             6965..8355
                     /gene="tcyP"
                     /locus_tag="KLH46_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8359..9175)
                     /locus_tag="KLH46_002114"
                     /pseudo
     CDS             complement(8359..9175)
                     /locus_tag="KLH46_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9452..9715)
                     /gene="cedA"
                     /locus_tag="KLH46_002115"
     CDS             complement(9452..9715)
                     /gene="cedA"
                     /locus_tag="KLH46_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBE0378795.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9898..12159
                     /gene="katE"
                     /locus_tag="KLH46_002116"
     CDS             9898..12159
                     /gene="katE"
                     /locus_tag="KLH46_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBE0378796.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(12206..12964)
                     /gene="chbG"
                     /locus_tag="KLH46_002117"
     CDS             complement(12206..12964)
                     /gene="chbG"
                     /locus_tag="KLH46_002117"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBE0378797.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPVNLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12977..14329)
                     /gene="celF"
                     /locus_tag="KLH46_002118"
                     /gene_synonym="chbF"
     CDS             complement(12977..14329)
                     /gene="celF"
                     /locus_tag="KLH46_002118"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBE0378798.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGHDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14434..15276)
                     /gene="chbR"
                     /locus_tag="KLH46_002119"
                     /pseudo
     CDS             complement(14434..15276)
                     /gene="chbR"
                     /locus_tag="KLH46_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(15284..15634)
                     /gene="chbA"
                     /locus_tag="KLH46_002120"
     CDS             complement(15284..15634)
                     /gene="chbA"
                     /locus_tag="KLH46_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBE0378799.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15693..17042)
                     /gene="chbC"
                     /locus_tag="KLH46_002121"
     CDS             complement(15693..17042)
                     /gene="chbC"
                     /locus_tag="KLH46_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBE0378800.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(17127..17447)
                     /gene="chbB"
                     /locus_tag="KLH46_002122"
     CDS             complement(17127..17447)
                     /gene="chbB"
                     /locus_tag="KLH46_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBE0378801.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17746..18084)
                     /gene="osmE"
                     /locus_tag="KLH46_002123"
     CDS             complement(17746..18084)
                     /gene="osmE"
                     /locus_tag="KLH46_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBE0378802.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18286..19113
                     /gene="nadE"
                     /locus_tag="KLH46_002124"
     CDS             18286..19113
                     /gene="nadE"
                     /locus_tag="KLH46_002124"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBE0378803.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            19343..20230
                     /gene="cho"
                     /locus_tag="KLH46_002125"
     CDS             19343..20230
                     /gene="cho"
                     /locus_tag="KLH46_002125"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBE0378804.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20190..20765)
                     /gene="ves"
                     /locus_tag="KLH46_002126"
     CDS             complement(20190..20765)
                     /gene="ves"
                     /locus_tag="KLH46_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBE0378805.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(20968..21453)
                     /gene="spy"
                     /locus_tag="KLH46_002127"
     CDS             complement(20968..21453)
                     /gene="spy"
                     /locus_tag="KLH46_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBE0378806.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(21783..22751)
                     /gene="astE"
                     /locus_tag="KLH46_002128"
     CDS             complement(21783..22751)
                     /gene="astE"
                     /locus_tag="KLH46_002128"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBE0378807.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALVCTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(22744..24077)
                     /gene="astB"
                     /locus_tag="KLH46_002129"
                     /pseudo
     CDS             complement(22744..24077)
                     /gene="astB"
                     /locus_tag="KLH46_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(24074..25552)
                     /gene="astD"
                     /locus_tag="KLH46_002130"
     CDS             complement(24074..25552)
                     /gene="astD"
                     /locus_tag="KLH46_002130"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HBE0378808.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTVGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(25549..26583)
                     /gene="astA"
                     /locus_tag="KLH46_002131"
     CDS             complement(25549..26583)
                     /gene="astA"
                     /locus_tag="KLH46_002131"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBE0378809.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDSATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(26580..27800)
                     /gene="astC"
                     /locus_tag="KLH46_002132"
                     /pseudo
     CDS             complement(26580..27800)
                     /gene="astC"
                     /locus_tag="KLH46_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            28246..29052
                     /gene="xthA"
                     /locus_tag="KLH46_002133"
     CDS             28246..29052
                     /gene="xthA"
                     /locus_tag="KLH46_002133"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBE0378810.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            29219..29927
                     /locus_tag="KLH46_002134"
                     /pseudo
     CDS             29219..29927
                     /locus_tag="KLH46_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            29932..30609
                     /gene="ydjY"
                     /locus_tag="KLH46_002135"
     CDS             29932..30609
                     /gene="ydjY"
                     /locus_tag="KLH46_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBE0378811.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            30624..31331
                     /locus_tag="KLH46_002136"
     CDS             30624..31331
                     /locus_tag="KLH46_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBE0378812.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            31331..31879
                     /locus_tag="KLH46_002137"
     CDS             31331..31879
                     /locus_tag="KLH46_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBE0378813.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            31889..>32211
                     /locus_tag="KLH46_002138"
     CDS             31889..>32211
                     /locus_tag="KLH46_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE0378814.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTL"
BASE COUNT         8270 a         8258 c         7625 g         8058 t
ORIGIN      
        1 gcatgacaag gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat
       61 taccccgatg ctcagacctg ggctgatagg tatgttcgca actgtgcaat tgctattctg
      121 cccctattac ctgtgggagt tccaggggct attttcgcgg gtattgcatc acgaatgcca
      181 tttgccccta tacatccatc ggcaatgttg ttaataatgg ctacaggcca gactcaacag
      241 cttattacat tattcaaaca gttgcccata ctccctgaaa aagaaatcat tgaaataata
      301 actgcgcaga atagcgttgg tacacctgct ttatttttgg ctatgatgaa cggacatact
      361 gacaacgtga aaatatttat gcaagaaatt cagtcactgg tagataatca catcattcat
      421 gaagataatc tggttaaatt actgcaaact aaaagtgcta acgaaacacc tggactttat
      481 atctccatgt tgtatggatt cgatgaaata atcgatatct ttctgaatac attaaccact
      541 ccaatagcac taagagcttt taaacaaaaa actggtgatg agtattttag ccatgaaaat
      601 acatgatggt gagccaggat tatacgccgc aatggaaaat aatcaccctt tgtgtgtcac
      661 acggttcctc tctaaaatta atggcatcgc ctttaaatac aagttgagca aagctaacat
      721 catggattta ttaaaaggag ctacagcaca gggaacccct gcattataca tcgccatgag
      781 caagggtaat gaagacgtcg tgttatctta tatatcgacg ctgggtgctt ttgcaaaaaa
      841 acattctttt agtcaacatc agttatttac actattggcc gctaaaaatc atgacaacat
      901 gtcagctgtt catatagcca ttcatcataa gcattataaa actgtagaaa catattatgc
      961 tgctataaat gtcatcagcc aaagcctgag ttttagtgct gatgaaataa agacgtattt
     1021 ataaccagct atttacgata tatatcgata cataatttta tttttttcat aaaacgcaga
     1081 aacagtgacc atgtgcacaa cgccacccga cggctgtaat aacaaaaaat cccttgatgc
     1141 ctgtcccttt tgttacactc cgttatcacg cacaagagat atgcaggaca ctggtatgcc
     1201 gactaaacgc tttgataaaa aacactggaa gatggtggtg gtgctactgg caatctgtgg
     1261 cgctatgttg ttgctacgtt gggcagcaat gatttggggc tgagcagtga caaacgaaga
     1321 cagccagacc gcataacacg gtccggcaga taattttaca tgctgtagcc cagcgataag
     1381 gttgtccgac ggtcggtatg ttccggtgca gactctggag gttcagagtt ccatgtgacg
     1441 ttgtaagcca ccttcagtcc aaaatgttca ttaatagcaa catttaatgc gctttcggag
     1501 ttcagcgttg tgtcttccgc gccaaagacc gaaacaccct gcgtaaattt agcgttgtca
     1561 gtcaactgcc atgcataagc cccggaggca tagcccagcg gctgggtttc actggcattg
     1621 tcggtatatt tgtcgtaacg cacacctgga ccgaattcaa agcggaaact gtgtaccggg
     1681 ccattcaaaa actgacgacc ataacccgcg gtcaacacat cgcgctctcg atagccgtta
     1741 taacggtctg tcagccagct tgcctgacca aataaatagt cataatcagt taaattaaag
     1801 cggctacgcc cgcccgccgc atatttttct gaagaacgct catcattaga agaagtatta
     1861 ctggcgttcc cccacagcga ccaggcagtg gtttgtccat accaggtcat ggtggtgtca
     1921 gccgtaagtg aggagctttt cgtattgcct gattgtgcaa gatatcctgc gttcagatta
     1981 ccttcgaaag gttttttagc gctggcagga tcgtccatga cagtaaaaac ggaatcatcg
     2041 gcagctgcat tcagtgacgc aaacatgccc cccgccaaca taacgatggc aggaactgtc
     2101 ttcaaaagct tcatttatca agagtccgta caacaaaaaa agagaccatc gcggtcccgg
     2161 aaactttctt aaggatcaaa gattagcgtc cctggaaagg taacgaatta taaaaaggcg
     2221 cgaataactt agcaatgtat tcttatttca ttttttgaat aagcatgtgg cgaaaacaga
     2281 tttttattta tatatattta tctgcaaaat tttaaataaa gctctaataa atcatattgt
     2341 taatttcttc actttccgct gattcggtgc cagactgaaa tcagcctata ggaggaaatg
     2401 atggtacgta tctatacgtt gacacttgcg ccctctctcg atagcgcaac aattaccccg
     2461 caaatttatc ccgaaggaaa actgcgctgt accgcaccgg tgttcgaacc cgggggcggc
     2521 ggcatcaacg tcgcccgcgc cattgcccat cttggaggca gtgccacagc gatcttcccg
     2581 gcgggtggcg cgaccggcga acacctggtt tcactgttgg cggatgaaaa tgtccccgtc
     2641 gctactgtag aagccaaaga ctggacccgg cagaatttac acgtacatgt ggaagcaagc
     2701 ggtgaacagt atcgttttgt tatgccaggc gcggcattaa atgaagatga gtttcgccag
     2761 cttgaagagc aagttctgga aattgaatcc ggggccatcc tggtcataag cggaagcctg
     2821 ccgccaggtg tgaagctgga aaaattaacc caactgattt ccgctgcgca aaaccaaggg
     2881 atccgctgca tcatcgacag ttctggcgaa gcgttaagtg cagcactggc gattggtaac
     2941 atcgagttgg ttaagcctaa ccaaaaagaa ctcagtgcgc tggtgaatcg cgaactcacc
     3001 cagccagacg atgtccgcaa agccgcgcag gaaatcgtta atagcggcaa ggccaaacgg
     3061 gttgtcgttt cccttggtcc acaaggagcg ctgggtgtcg atagtgaaaa ctgcattcag
     3121 gtggtgccac caccggtgaa aagccagagt accgttggcg ctggtgacag catggtcggc
     3181 gcgatgacac tgaaactggc agaaaatgcc tctcttgaag agatggtccg ttttggcgta
     3241 gctgcgggga gtgcagccac actcaatcag ggaacacgtc tgtgctccca tgacgatacg
     3301 caaaaaattt acgcttacct ttcccgctaa caaaaacatt cccccagcat tgggggaatc
     3361 accaccaacc tgtcggcaac gcgtttctcc gactatgctc aaaagtcatg tgataacaaa
     3421 ggggtgaact atggccagtg gcgatcttgt ccgttatgtc ataaccgtca tgttgcatga
     3481 ggatacattg actgaaatta acgagttgaa taattacctg actcgcgacg gttttttgct
     3541 caccatgacg gatgatgagg gaaatatcca tgagctgggg actaacactt ttggacttat
     3601 cagtacccaa agtgaagaag aaattagaga actggtttcg gggcttaccc aaagtgcaac
     3661 cggcaaagat cctgaaatca ccatcacgac ctgggaggaa tggaatagca acagaaaata
     3721 aatggttttt gggcaataat cagtctgtgg tgtgcgttag ctcgtgtttt tacaccgcat
     3781 tcttgcgcta accttatgat ctggcagaca acatgggaga gacatcatgt ggcaggcaat
     3841 cagtcgtctt ttgagcgagc agttaggtga aggcgaaatc gaactgcgta atgaactgcc
     3901 tggcggagaa gtccatgccg catggcattt gcgctatgca ggacatgact ttttcgtcaa
     3961 atgtgatgaa agggaactgc ttcccggttt taccgccgaa gccgaccaac tggagttact
     4021 gtcgcgtagt aaaaccgtca ccgtgcctaa ggtttgggca gtaggcgctg accgtgacta
     4081 cagttttctg gtgatggatt atctcccacc tcgtccgctg gatgcgcata gcgcatttat
     4141 tcttggtcag caaattgcgc gtttacatca atggagtgac caaccacaat ttggcctcga
     4201 ttttgataac tcgctctcca caactccaca gcccaacacc tggcaacgtc gctggtcaac
     4261 gttttttgct gaacaacgga ttggctggca gttggaactg gcagcagaga aagggatcgc
     4321 tttcggcaat atcgacgcca tcgtcgagca tattcagcag cgtctggcct cgcatcaacc
     4381 gcagccttct ctgttgcacg gcgatttatg gtccggcaac tgtgcgctgg gtccggatgg
     4441 cccgtacatt ttcgaccctg cctgcgactg gggtgaccga gagtgcgacc tagcgatgtt
     4501 accgctgcat actgaacagc cgccacaaat ctatgacggc tatcagtcag tatccccgct
     4561 acctgccgat ttccttgaac gtcaaccggt ttaccaactc tacacgctgt taaatcgtgc
     4621 aagattattt ggcggtcagc atttggttat tgctcagcag tcattggata gattattagc
     4681 agcatgatat gggttgagga taatggccgc tccgtgcggc cttttgatta aataaaccca
     4741 agcagagaaa agaacacata acctgcagca atacaaataa ccggcaggat atatagtgag
     4801 aaaaactgca ggaaaatagt atggtgcgga acaacaatac gggactcaat ttgttcacgc
     4861 gttagcccct cttccccttt ggctttttcc agaatgagtt gatcttcaac accttcccgc
     4921 aagaagcgcg cctggcgact catccgggca ccggaatcct gcaacgccag tccgacaaaa
     4981 atcaggatga aaatcaccca gaacataacg ttcacaccac cattaaaatt aggggtcggg
     5041 gagttatacc agaacagatt caaaaaaggc gtattcgcct gcatcatatc gatcatgaca
     5101 tgagcaaagt cgagcatgac cgcattaatg ccttcctggt tttcctgccg ggtattcata
     5161 aatttcagca aagaaatcaa tgtagagatc aatgcaggta taaaaatcac ccaacccaaa
     5221 atccttttca aaacagcaat gcgtccagct tgttgatacg tcatgagttc tccttgatta
     5281 agacgcgtcg tttcacttag tttacctgta gatatctgtt ttcgcccatt ctttaaaggc
     5341 gatatgatag gcgcttaatc ataagcacgg cttaatacct tacacataat gctctaaagg
     5401 agaggttgta atgtcaaccc cgcgtcagat tcttgctgca atttttgata tggatggatt
     5461 acttatcgac tcagaacctt tatgggatcg agccgaactg gatgtgatgg caagcctggg
     5521 ggtagatatc tcccgtcgta acgagctgcc ggacacctta ggtttacgca tcgatatggt
     5581 ggtcgatctt tggtacgccc ggcaaccgtg gaatgggcca agccgtcagg aagtagtaga
     5641 acgggttatt gcccgtgcca tttcactggt tgaagagaca cgtccattat taccaggcgt
     5701 gcgcgaagct gttgcgttat gcaaagaaca aggtttattg gtgggactgg cctccgcgtc
     5761 accactacat atgctggaaa aagtgttgac catgtttgac ttacgcgaca gtttcgatgc
     5821 cctcgcctcg gctgaaaaac tgccttacag caagccgcat ccgcaagtat atctcgactg
     5881 cgcagcaaaa ctgggcgttg acccactgac ctgcgtggcg ctggaagact cggtaaatgg
     5941 catgatcgct tctaaagcag cccgcatgcg ttccatcgtc gttcctgcgc cagaagcgca
     6001 aaatgatcca cgttttgtat tagcaaacgt caaactctca tcgctgacag aactcaccgc
     6061 aaaagacctt ctcggttaat gaccaggggc agtgatcacg ctgcccctgg ttctttatct
     6121 gaatttgcat tcaactgacg gattaatcgt caatttaaga gaaagagtta caccgtcacc
     6181 acttccgtgc actgtataaa aatcctatac tgtacgtatc gacagtttag tgagttttat
     6241 catgacggcg gaaggtcacc ttctcttttc tattgcttgt gcggtatttg ccaaaaatgc
     6301 cgagctgacg cccgtgctgg cacagggtga ctggtggcat attgtccctt ccgcaatcct
     6361 gacgtgtttg ttaccggaca tcgatcaccc aaagtcgttt cttgggcagc gattaaaatg
     6421 gatatcaaaa ccgatcgccc gcgcttttgg gcatcgtggt tttacccaca gtctgctggc
     6481 ggtatttgcg ctgctggcaa ccttttacct taaggttccg gaaggctggt tcattccggc
     6541 tgatgcgcta caaggaatgg tgctgggtta tttgagccac atacttgccg atatgctgac
     6601 acccgccggt gttcccctgc tctggccatg ccgctggcgt ttccgcttgc ctatcctggt
     6661 tccccaaaag ggcaaccaac tggaacgttt tatctgcatg gcattatttg tctggtcggt
     6721 atggatgccc cattcattac ccgagaacag cgctgttcgt tggtcatcgc aaatgatcaa
     6781 taccttgcag atccagtttc atcggcttat taagcatcag gttgaatact aaaaaggcaa
     6841 aaatcacctt tctggaataa gcaattccat ttgaatataa gagccagctc acagttctgt
     6901 taatcttgcg ccaacactat gactgctacg cagtgataga aataataaga tcaggagaac
     6961 ggggatgaac tttccattaa ttgcgaacat cgtggtgttc gttgtactgc tgtttgcgct
     7021 ggctcagacc cgccataaac agtggagtct ggcgaaaaaa gtgctggtgg gtctggtgat
     7081 gggtgtggtt tttggccttg ccctgcatac catttatggt tctgacagcc aggtacttaa
     7141 agattctgta cagtggttta acatcgttgg taacggctat gttcaactgc tgcaaatgat
     7201 cgttatgccg ttagtcttcg cctctattct gagcgcggtt gcccgtctgc ataacgcatc
     7261 tcagttaggc aaaatcagtt ttctgaccat cggtacgctt ttgtttacca cgctgattgc
     7321 ggcgctggtc ggtgtgctgg tcaccaacct gtttggtttg acggctgaag gtctggttca
     7381 gggtggtgca gaaactgcac gtctgaacgc cattgaaagt aactatgttg gtaaagtctc
     7441 tgatcagagc gttccgcagc tggtcttgtc ctttatcccg aaaaacccgt ttgccgatct
     7501 taccggagcc aatccgacgt caatcatcag cgtggtaatt tttgccgcat tcctcggcgt
     7561 agctgcattg aaactgctga aggatgatgc gccgaaaggg gaacgcgtct taaccgctat
     7621 cgatacccta caaagctggg tgatgaaact ggttcgcctt gtcatgcagt tgacccctta
     7681 cggcgttctg gctctaatga ccaaagtggt tgcaggttct aacttgcaag acatcatcaa
     7741 actgggaagt ttcgttgtcg cgtcctacct cggtctgctg attatgtttg ctgtgcatgg
     7801 cattctgctg ggcattaatg gcgtgagtcc gctgaagtac ttccgtaagg tatggcctgt
     7861 gctgacgttt gcctttacca gccgttccag tgctgcgtct atcccactga atgtggaagc
     7921 acaaacgcgt cgtctgggcg ttcctgaatc catcgccagt ttcgccgcct ctttcggtgc
     7981 aaccattggt cagaacggct gcgccggttt gtatccggca atgctgcggt gatggttgcg
     8041 cctacggttg gcattaaccc gctggacccg atgtggattg cgacgctggt cggtattgtt
     8101 accgttagtt ccgcaggcgt tgccggtgtc ggtggtggtg caactttcgc cgcactgatt
     8161 gtgctgcctg cgatgggcct gccagtaacc ctggtggcgc tgttaatctc cgttgaaccg
     8221 cttatcgaca tgggccgtac ggcgctaaac gttagtggct cgatgacagc tggcacgctg
     8281 accagccagt ggctgaagca aaccgataaa gccattctgg atagcgaaga cgacgccgaa
     8341 ctggcacacc gttaattctt atgctggcaa cggtccgttt tgtatagggg ccgttgcttt
     8401 actttttaaa tgttccatcg cggcgatgca tactgactca tatttaaaca gatcgtcagc
     8461 gaaaaaaatg tccaacttat caatacattc ctggctcaat gaatgtgcct gtagtgagaa
     8521 gcttaaaacc tcatacccga gacgttttct aagtgaacta tctggaatac tcataatatc
     8581 ttcaagcaca ccacttccca ttcccggaag atttagtgga ttaaaaagaa gattgatatt
     8641 aagcattgat attatcgaag gaaactttag gagactctca ttttttttga atcgcagcaa
     8701 aaatgctccc ggcaggatgt atataatttt tttcatagta tatagtcctt aaataatgaa
     8761 taaccaacct attttttata ttgatcaaat tcgtcatttt ttcgggtcgg aattcacagg
     8821 taaaatccat cccaacaaaa ttctcattaa tatctcttat ccgaaactca ctgccataaa
     8881 gattattaat tatcactcta tatttaacat tgtcctgcag atacgtatca aaactaagtg
     8941 ctggcgctcc tagctttgag gcatcatagt taatgttaga catataggca atcatacgat
     9001 tgaagttcga ctctgctatt tcatcgcagt cacattgacc cgttgatata aagaaagtga
     9061 gtgccgccat ggtagactca agcctttctt taaaagagtc gaaagccagg cttttaatat
     9121 ttaaatcacc ataattactc tgtattaagt ttgtagaaaa catctcccgc ctcatattgt
     9181 taacaaaatt attatcttat ttaaatctaa gtcatttaca atataagttt aagagcgacg
     9241 ccacaggatg aactatcaaa aataactcat catgattagc aaaacttaac cattttaaaa
     9301 taaataaata attaaagaaa aaagatcact tatttatagc aatagctcgt caaaggcagc
     9361 tttttgttac aggtggtttg aatgaatgta gcaacgaaat gcagaatttc aggtcctgta
     9421 actcccggca aaaccgggag gtatgtaatc cttactcagt cacttcccct tcctggcgga
     9481 tctgatttgc ccaacgttgg gcagattcag gcacagtaaa cgccggtgag cgcagaaatg
     9541 actctcccat cagtacaaac gcaacatatt tgccacgcag catccagaca tcacgaaacg
     9601 aatccatctt tatcgcatgt tctggcggcg cgggttccgt gcgtgggaca tagctaataa
     9661 tctggcggtt ttgctggcgg agcggtttct tcattactgg cttcactaaa cgcatattaa
     9721 aaattagaaa aactgtagtt tagccgattt agcccctgta cgtcccgctt tgcgtgtatt
     9781 tcataacacc gtttccagaa tagtctccga agcgggatct ggctggtggt ctatagttag
     9841 agagtttttt gaccaaaaca gcggcccttt cagtaataaa ttaaggagac gagttcaatg
     9901 tcgcaacata acgaaaagaa cccacatcag caccagtcac cactacacga ttccagcgaa
     9961 gcgaaaccgg ggatggactc actggcacct gaggacggat ctcatcgtcc agcggctgaa
    10021 ccaacaccgc caggtgcaca acctaccgcc cctgggagcc tgaaagcccc tgatacacgt
    10081 aacgaaaaac ttaattctct ggaagacgta cgcaaaggca gtgaaaatta tgcgctgacc
    10141 actaatcagg gcgtgcgcat cgccgatgat caaaactcac tgcgtgccgg taaccgtggt
    10201 ccaacgctgc tggaagattt tattctgcgc gagaaaatca cccactttga ccatgagcgc
    10261 atcccggaac gtattgttca tgcacgcggc tcagcagctc acggttattt ccagccatat
    10321 aaaagcttaa gcgatatcac caaagcggat ttcctctcag atccgaacaa aatcacccca
    10381 gtatttgtta ggttctctac cgttcagggt ggagctggct ctgccgatac cgtgcgtgat
    10441 atccgtggtt ttgccaccaa gttctatact gaagagggta tttttgacct cgttggcaat
    10501 aacacgccaa tcttctttat ccaggatgcg cataaattcc ccgattttgt tcatgcggta
    10561 aaaccagaac cgcactgggc aattccacaa gggcaaagtg cccacgatac tttctgggat
    10621 tatgtttctc tgcaacctga aactctgcac aacgtgatgt gggcgatgtc ggatcgcggc
    10681 atcccccgca gttaccgcac catggaaggc ttcggtattc acaccttccg cctgattaat
    10741 gccgaaggga aggcaacgtt tgtacgtttc cactggaaac cactggcagg taaagcctca
    10801 ctcgtttggg atgaagcaca aaaactcacc ggacgtgacc cggacttcca ccgccgcgag
    10861 ttgtgggaag ccattgaagc aggcgatttt ccggaatacg aactgggctt ccagttgatt
    10921 cctgaagaag atgaattcaa gttcgacttc gatcttctcg atccaaccaa actgatcccg
    10981 gaagaactgg tgcccgttca gcgtgtcggc aaaatggtgc tcaatcgcaa tccggataac
    11041 ttctttgccg aaaacgaaca agcagcattc catccaggtc atattgttcc tggtctggac
    11101 ttcaccaacg atcccctgtt gcagggacgt ttgttctcct ataccgatac acaaatcagt
    11161 cgtcttggtg ggccgaattt ccatgagatt ccgattaacc gcccgacctg cccttaccat
    11221 aatttccagc gtgacggcat gcatcgtatg gggatcgaca ctaacccggc gaattatgaa
    11281 ccgaactcga tcaacgataa ctggccgcgc gaaacaccac cggggccgaa acgcggcggt
    11341 tttgaatcat accaagagcg cgtggaaggc aataaagttc gcgagcgcag cccatcgttt
    11401 ggcgaatatt attcccatcc gcgtctgttc tggctaagtc agacgccatt cgagcagcgc
    11461 catattgtcg atggtttcag ttttgagtta agcaaagtcg ttcgtccgta tattcgtgag
    11521 cgcgttgttg accagctggc gcatattgat ctcactctgg cccaggcggt ggcgaaaaat
    11581 ctcggtatcg aactgactga cgaccagctg aatatcaccc cacctccgga cgtcaacggt
    11641 ctgaaaaagg atccatcctt aagtttgtac gccattcctg acggtgatgt gaaaggtcgc
    11701 gtggtagcga ttttacttaa tgatgaagtg agatcggcag accttctggc cattctcaag
    11761 gccatgaagg ccaaaggcgt tcatgccaaa ctgctctact cccgaatggg tgaagtgact
    11821 gcggatgacg gtacggtgtt gcctatagcc gctacctttg ccggtgcacc ttcgctgacg
    11881 gtcgatgcgg tcattgtccc ttgcggcaat atcgcggata tcgctgacaa cggcgatgcc
    11941 aactactatc tgatggaagc ctacaaacac cttaaaccga ttgcgctggc gggagacgcg
    12001 cgcaagttta aagcaacaat caaggtagct gaccagggtg aagaagggat tgtggaagct
    12061 gacagcaccg acggtagttt tatggatgaa ctgttaacgc tgatggcaga acaccgcgtg
    12121 tggtcacgca ttcctaagat tgacaaaatt ccggcgtaaa tatggataag gtcacgtccc
    12181 ctggggagtg accttaccac cttacttaca catcaagata actacccaac cgataaccac
    12241 gctcagcaat tgcatatttc aacgacgctg atgtcagcac ttccagctcc gttaaccgtg
    12301 ggaaacagta agcactctga cggatagtat tatcgataaa cgccggatga cacatcacct
    12361 ccagcgaagg atcaccccga tggcttgcgt catccagcac ttgcaggaac agtgcctcgc
    12421 taatctcttc accataaaac gcgctactga atccctgcga actgcgcaga ttaaccggta
    12481 aatcaccagc gttagatagc ggctgacggt cgatgcgcaa cgcaatcccc tcttcagccg
    12541 caaacctggc aacaatcggg aaaatctgcg ggaacatatg cacatgatga tggctatcaa
    12601 gatgcgtagg tttgcgtcca aatagctcaa tgaaacgcaa atactgactg gcaagctcct
    12661 gagtaatttc ttccagcggt aaagcatctt cttctgccaa ctgccagatc cattttccca
    12721 gcacaccatc gcgggttaac cccggcatag ctgtcagtgg cttgcccata gtaaggacaa
    12781 agtgcatccc tatggccaga ctcggttcat cacgactcaa ctgcaccgca tggtcaatag
    12841 cctgcccatt caccagcgcc gtcgtcgatg tgacaatccc attgcgacag gcctcgataa
    12901 tgccgtagtt ctgacctttg cttaagccaa aatcatcggc attaacaatc agtaagcgtt
    12961 ccataatcag cctcggttaa tgtgcttttt taagctctgc gatgcagtcg gcaaaatttg
    13021 gcagccattt ctcgtgcgcc agaatcatct cgcgtgccag cagctcagca tcgcgatcgg
    13081 aatgcaccaa cggactaagg tttagcgcca gtaacacatc gttaaattct ccgctaagtg
    13141 cggcgttgct ggcagcaatc tcgaagcctt taatggtgtg aatcagcccc atcactttat
    13201 catcgaaatg cgtaatgcgc ggatgtggcg tcgcgccatc gtgccccagc ttacaggtca
    13261 tttctaccgc ccagtctgcc ggaatattat caatatgccc atgatgcggg atattaacgt
    13321 aatgttcagc ttgcttgtcg ttgtagatag cgttgatcac ttcgcacgct gcatcagagt
    13381 aataagcccc accgcgctgt tccagttctt tcggcttaac tttcaactcc gggtttttat
    13441 acagctcaaa aagttgtttc tcgactttct gtactacctg cgctcgtgcg ccgcctttgt
    13501 agtattcgcc catttcaata gccagcatct ctttttgctt gaagtaataa agcaaataag
    13561 aacacggcaa cagattcaga gaacgaatta agccttcgct aaatggcaga tcgaaaatat
    13621 ttttaacgcc agatactttt aactgcccgg atgccacacc atcaagcaat tcggcaaagc
    13681 gcgacttgcc atttaccagc acatccttaa tgaacaccat atggttgagg ccaaacaaat
    13741 cgatagataa atcatcgctg tctttcagca tcagaacatc gtgaataaac atcttcatgc
    13801 cgatcggaat attacacacg ccgataaaac gtttaaatcc ggtatgacga taaacggctt
    13861 cagtaaccat tcccgccggg ttagtgaagt taatcaccca tgcattcgga caaagttctt
    13921 cgacatcttt tacgatgtca aaaatcaccg gaatggtacg cagaccttta aacaaaccgc
    13981 ccgcgccgtt ggtttcctga ccaagataac catgacttaa tggaatacgt tcatccagtt
    14041 cacgcgccgg taattggcca acgcgcagtt gggtagtaac gaaatcagca tctttcaatg
    14101 cttcgcggca atccagcgtt ttataaagct tcatcgggac gccagcgtta tcaatcatcc
    14161 gttggcagag atcgaaaata atatccagtt tagctttgcc accctcgaca tccaccagcc
    14221 ataattcgct gaccggcaat tcgtgataac gcttaataaa tccttccagt aactccgggg
    14281 tatagctgct cccgccacca atagtgacga cttttaattt ctggctcata atttctccct
    14341 tcagtacaga atactgatat ctggcatatc tgcccccccg gacataaata atccagcaac
    14401 aggacagata tgtgaattgt caggtataac gacttactga ttaaattcag tcaatttctt
    14461 acgatatctc ttaggcgtaa aggacgttaa tttcttaaac gttttaataa acaaactcgg
    14521 gctactataa ccggcctcaa acgcaatatc cgtcacggaa tagttggtca tttccagttg
    14581 tttttttgca aaattaatac ggatctcatt aataatctgc attggtgttt tgccataata
    14641 tcgttgagtc gctcgcgtca aatattcctg tgatttggct gacaacgtca ccatattctc
    14701 cagcgccgat tcactaaact gctctttatc atgcatcttt tctaccgtac ttttcagcca
    14761 ctacggtaca tcatcaatca cctgttcttc gcggtaatga cgtaaacggt taatgacata
    14821 aaaagtaacc atctcaacaa actcttccag ccctgtttcg cggaaattta atgaagaaat
    14881 cactgtttcc acataggtga gaaaagcgtt attggtccgg tatacctgcg aagcaacaaa
    14941 gcaataaggc aacaacggca ggtaatgctg ctcaaaaaag cgtttactga tcccaacgtt
    15001 caatatgcgc gtggcaccaa actcataaaa actttgatgg tgcgaaccta acggaataaa
    15061 aacaaaatcg ccccgttcca gtaacacgcg cttaccgtta atctcctgga aataacgccc
    15121 ggttaatact agagtaaatt cataatagtc gtgctgatgc agtccgctga tactctcagt
    15181 tttgttatag ataaacacat ggaagttttt gccattaaac aactgctgtt ctcgggcagt
    15241 ggcaatttcc ggcgcgttaa tcactggctg catcatcgac tccttatgcc ttcagttttt
    15301 catgaagctc aattaattca gtaatcagtt cacgcgcaag catggacgtc attaaatgat
    15361 cctgagcgtg gaccagcacc agactcactt tcatcttacc ttcgcccgca tcgccttcaa
    15421 tcagtttcgt ctgtaccaga tgcgcttcat tcaatgccat ccgtgactga tccatcatgg
    15481 ctttcgctgc ggcaaaatcg ccctgcttcg cctgtttcaa tgcggcatac gccaggctac
    15541 gcgcttgtcc agagttgatg ataagcccca tcaccacttc ttccagctct tcagcttctg
    15601 tttgcgtatc aggaatatta tcgagatcca tcatacatcg ttcctctctc ttcttaccgg
    15661 caagattacc cgtaccggca tcgattaaaa tttcaggggt tagcgatatc ttcttcgctc
    15721 tcttctttat caatcgcatt ttgtgctttg ttagccacca caacaaaggg cagataaatt
    15781 aacgttgcga tgccaaggtt gaagagtgcg accagcaatg cggcgacgct accgttggtg
    15841 ttaaagaagg ctcccagacc ggttggcatg gtccacggtg caatattggt caccggagga
    15901 atgatgccca tgtagtacgc tgcgagggtg attgccgcca gaatcggttg taccagtaca
    15961 aacgggataa acatcaccgg gttcatgata attggcagac caaacagaat cggttcgtta
    16021 atctggaaga tgccggacgg cagcgccagt tttgccacct gacgataatc agcacggcga
    16081 gaggcgataa agatagccag gatcaggcct aaagtcgcac cactgccccc aaggaaaata
    16141 aaggagtcca gcatcggctt ggcccagata tggaaggtct taccggctgc cagcgccgct
    16201 tcgacggaac catattgctg ataggtcgcg atattttcca gcgcccacgg cgtcataatg
    16261 ccgttgtcca gtgcggtcag cgccagcgcg ccatgaatac cgaagaacca gagcagtgga
    16321 acaaagatca cataggccca gcccaccacg ctacccaacg atgccagtgg ggttgagatg
    16381 gtatccataa tgatctgatg gaagttggtg ccccaggtat tcaacgccca ggcaataatc
    16441 cccatcacgg aaagaataat aaagccggga attaatgccg agaaagaacg cgatactgaa
    16501 gcaggtacgc tgtcgggcag tttaatgacc caattgcggc ggacaataaa ggtaaacatt
    16561 tctgccacca ccaggccaat aataatcccg gagatgatat tcgccccacc taaccagttt
    16621 gcgccaaccg cataggcctc accgacacta tatggggtga cggtcataaa tgctgcaacg
    16681 gataacaacc cagccgccag cgcatcgact ttacgctctt ctgccagcgc catgccaata
    16741 aagaacggtg ccattaaaga cattattcct aatgttccgt tatatacgtt gccgccaata
    16801 cctttcagac cattaagtgt ttcaatggtt gaggcgtcga ggcgaatacc taaggaataa
    16861 aaaaacgacc cctccccaaa gcttagaaaa acgttgttaa ttaatacaaa catggccccc
    16921 gcaagggtta acggcattaa gcgaataaag ccatttttga ttgcattaac gtgtggctgc
    16981 tttcctattt taactgcaaa agggaggagt accttttcaa gcgatgcaat aacattactc
    17041 atagaaaaat acccttaaaa accgcaattt aaatattacg gtattgattt atgaaataac
    17101 tctttgacgg gaaaatttaa aataaattaa tttgctgcgg cttttttaat cgctgcaacc
    17161 gcagccttaa gcacgcctaa accatcgact ttgccataaa gcagcgagtc aattacttca
    17221 accggtttgt tgggtaacaa acgctggatt tcgggcaaca tataagcaat ctgcggccct
    17281 aataacacga catcggcatt ctgacctttt tcaccagcca gtgtttccgg aaatgcttca
    17341 ataatgaccg gaacttcata tttttctgcc tgtgcgcgca tttttgatac cagtaaagag
    17401 atagacatgc ccgcagaaca aaacagataa atgtgtttct tttccataaa actgccctca
    17461 tcgacgatta tctgtcagcc agacactccg caagccttga cctgcttcca tgctctgggt
    17521 aacttgcgaa accaacatga ctttttttgt ggctgaaagg agtatacggt atagacctga
    17581 gcaatagtat ttctttgacc ttataaattg tttctccttg acctgtggtt atgaccctct
    17641 tcacatttcg ggcaaataat tcgcgaagat aattaagaac tcaggccaaa aaaaaccggc
    17701 gcaatggccg gtttccgttg ttactcaagc tttcagacga attgattact tcgcagcctg
    17761 tggatcagtg tcgtattcag cacaggtctg ataaccggag ttgatgacat gtccggtatc
    17821 atctaacgcg acaaagtagg tttctgcttt accatcacgt tgacccagga tgtaggtctg
    17881 gcaagtaccg cgagcatgga tcatgctcac ttcagacgaa ggtttacccg caatttgtgc
    17941 aacctgcgcc cggctcatgc cttttttgac gtctttcacc acaggctgta caaactggtc
    18001 tttggtacga tcataagctg tacaacccgc cagcatggtt aataccgccg ctgcactcag
    18061 aattcctgcc atattcttgt tcatattccg tcctcttgtt tatcagcgtg ttagataagc
    18121 ctggaataca ttgggcgctt tttcaagccc gtgaacgaaa cggctccgct ttcagaggat
    18181 tcctgtatga cgttttaacc accattcagc ccgctgtcgc ttgtcgtttc agtagcaacg
    18241 ggttagcttt aaggaagttt tgtcttttct gtctggaggg gttcaatgac attgcaacaa
    18301 caaataataa aggcgctggg cgcaaaaccg cagattaatg ctgaagagga aattcgtcgt
    18361 agtgtcgatt ttctgaaaag ctacctgcaa acttatccgt tcattaaatc actggtgctc
    18421 gggatcagcg gcggtcagga ctccacgctt gccggaaagc tgtgccagat ggcgattaat
    18481 gagctgcgcc aggaaactcg caacgaatca ctgcaattta ttgccgtacg cctgccctat
    18541 ggtgttcagg ctgacgaaca agattgccag gatgccattg cctttattca accggatcgc
    18601 gtattaaccg ttaatatcaa gggcgcggta ttggctagcg agcaggcatt gcgggaagca
    18661 ggcattgaac tgagcgattt tgtccgtggc aatgaaaaag cgcgtgagcg gatgaaagca
    18721 caatatagca ttgcgggtat gaccagcggt gtcgtggtgg gcaccgatca tgcagcagaa
    18781 gccattaccg gattcttcac taaatatggt gacggcggta cggatattaa tccgctgtat
    18841 cgtctcaaca aacgccaggg taaacagtta ctgacggcat taggttgccc ggaacacctt
    18901 tataagaaag ccccaactgc cgatcttgaa gatgatcgcc cttctctgcc agatgaagtg
    18961 gcactcggcg tgacctatga caatatcgac gactatctgg aagggaaaaa cctacctgaa
    19021 caggtcgcca gaacaataga gaactggtat ctgaaaaccg aacataaacg ccgtccgcca
    19081 attaacgttt tcgatgattt ctggaaaaag taataatttg cacctattgg attgtgcgaa
    19141 aaagagtaat ttgttcacgc cggatgcggc gtgaacgcct tattcgacct acaaaactat
    19201 gcaaattcaa tacattgcag gagtcgaata ggcctgacag gcgtagcacg tcagacggtg
    19261 taacctttgt catcaacccg cctctttttt aatcgcctcc cgcctgttac actggataga
    19321 taaccagcat tcggagtcaa cagtggtacg gcgtttaact tctccgcggc tcgaatttga
    19381 agctgcggca atttatgaat atcccgaaca tttacattca ttccttaatg acttacccac
    19441 ccgaccaggg gtgtatctgt ttcatggcga aagtgacacc atgccgctct atatcggcaa
    19501 aagcgttaat atccgcagtc gggtgctttc tcatttacgc accccggatg aagccgccat
    19561 gctacggcaa tcccggcgaa ttagctggat ctgcaccgca ggcgaaatcg gcgctctgct
    19621 ccttgaagcg cgattaatca aagaacaaca gccgctgttt aataaacggt tgcgccgcaa
    19681 tcgccagctc tgtgccctgc aattaaatga aaagcgcgtc gatgtggtgt atgccaaaga
    19741 ggtggatttt tcacgagccc ccaacctgtt tggcctgttt gccaataggc gcgcagcttt
    19801 gcaagcattg cagagcatcg ctgatgaaca aaaactttgt tatggcctgt tgggactgga
    19861 accgttaagt cgcggtcgtg catgttttcg ttcagcgcta aaacgttgcg ccggagcatg
    19921 ctgcggtaaa gagagccatg aggaacatgc gctacgcttg cgccagtctc tggagcgttt
    19981 gcgagtggtg tgttggcctt ggcaaggtgc ggtggcactg aaagaacagc acccggaaat
    20041 gactcaatat catattattc aaaactggct gtggctgggg gcggttaatt cgctggaaga
    20101 agcgacaacg ttaattcgga cacccgccgg gtttgatcac gacggttata aaattctttg
    20161 taagccgctg ctttccggta actatgaaat tactgaactt gatccggcga atgaccagcg
    20221 agccagttga tttcactgaa caacagttta ccttgcggtt gtaaaagccg cagggtgtgc
    20281 ctgccatcga accagcacgc gccctggtcg gtagtgagca gtttgtcgcc cagttgccac
    20341 gccccgttaa tcacaaacac caccccgccg cgtgagccaa aggtagtaaa cgtacgttcg
    20401 gcaattctga ctttcgcctt acagacatcc aggcgcgtca taatattgaa atccatcgac
    20461 atctgccctg ccgtcagttt cgcttttacc acctggtccg ccgtaaaggc aaaaggctgt
    20521 aacggtttta aggtatggtt aaagcggtct gcgctttcaa ggagcatctc gccgccttcc
    20581 agcaacgtca ctatcctttc catgccggga aacaaagaaa actccccatt cgccgcgatg
    20641 gatgcaatgc tggcacgcca gtaaaaatca cgttttgccg gtgggaaagt gcaaatttcg
    20701 cgcgtttcac cggcagcgtt tcgccacaga ttcaccgaca ttttacgcat atcaaagtat
    20761 tccatactcg ctcccggccc gttcttttca ttgttatcgt tcaggtaacc gtggatgatt
    20821 atcggcaact cgggtgattt acgttagtgg tgatcaggct ttttttacaa ctgtttgaga
    20881 agagaaaaga aaaccgccga tcctgtccac cgcattactg caaggtagtg gacaagaccg
    20941 gcggtcttaa gttttttggc tgaaagatta ttcagcagtt gcaggcattt taccttttgc
    21001 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
    21061 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
    21121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
    21181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
    21241 gcctttcatg atttcgtgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
    21301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
    21361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
    21421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
    21481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
    21541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
    21601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac gatgattttg
    21661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
    21721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
    21781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
    21841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
    21901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
    21961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
    22021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
    22081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccaacgt acagaccagc
    22141 gcgccaaaat gtctgacgct gaaatgggta aacgtaccac caggctcctg atggaacacc
    22201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
    22261 cgttgcggta atacaccgaa ctgcggatgc aaggagccac gaattgcggt atgtagatca
    22321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
    22381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
    22441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg attcccgagg
    22501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
    22561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
    22621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
    22681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
    22741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
    22801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
    22861 ggcagcagta agacgatcgc ggtagtaacg atccacccag tcattgagca cattaaacaa
    22921 ggtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatacccc
    22981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta agtcaaagac
    23041 ttttagttca ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
    23101 ggcgtgctcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
    23161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
    23221 aacttctatc gccataaaac cgttcacccg cgtgccagta actgtgactg gcgagcgaac
    23281 gcctgttggt ggcaaaacag cacctggcgg ttactcacgg caatcacgtc attatgaaaa
    23341 acgctctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg gggattcacc
    23401 tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg ataacgggaa
    23461 ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gtagcatacc cggttcaccg
    23521 taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa cgctacctgc
    23581 ggcaacgccg aatggacgct aaatttctct tcgtccttaa aaatcgcttt taacagcgat
    23641 tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc aacggtgaga
    23701 tgcactttgc catccagcgt atcggcagat ggcgcgatcg ttgccgcatt ggctacccac
    23761 attggcgaag cggaactgac gctggaaagc cagtgcggtg cctggcgtgc aactttttcc
    23821 agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat aaacggacgc
    23881 tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggcttt cattttcagt
    23941 aagccctgtt ttgccgccag tcgcgggtta gacacctgaa aacggtgacg ggtagaggct
    24001 tcattaccaa acgacaggcc cgcgtaatga tgcgtcagcc ctaccagccc gtcgaaatta
    24061 acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg ttaagcgtgg
    24121 cgggcaatgt taacgagtcc gactccaggc tcgccatagg ccatgcgcag taatctgcgg
    24181 cataccaggc gctggggcga tggttaccgg aagcaccaat gccgccgaat ggggcggtac
    24241 tggcagcacc agtaagcggt ttgttccagt taacaatccc cgcccgcgcc tccagcaaca
    24301 gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca aaagagtccg aagcgagtgt
    24361 tgttcgccat ccgaatcgcc tcatcaaaag tatcataacg ccagacgcgc aaaagcggcc
    24421 cgaacacctc ttcatccggc acaccgccaa cgcccgtcat ttcaatgatc cctggtgtca
    24481 gcaacgatgt ccctgcttgt aataagcgcg gcgcaagcag ggttcgtccg cccatcgctt
    24541 ccagttgctg ccaggcagta accacctgct gtgcggcctg ttcagaaatc agcccgccaa
    24601 taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg acggcaacca
    24661 gactagcaag aaacgcatcg ccctgcgctc cgcttttgag caataaacgg cgggcgcagg
    24721 tgcagcgttg cccgactgtg acaaacgccg actgaatggt cagatggaca gccgcgtcga
    24781 tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg gcgagaattt
    24841 tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg ctaccggtaa
    24901 acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca cgcccgccct
    24961 gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc attaccgctt
    25021 cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct gccagcaatg
    25081 ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca aacaccgcca
    25141 gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca ctacgctgct
    25201 cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc atcgccgtca
    25261 cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc gcggttaatt
    25321 cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga cgttcagcca
    25381 atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc tgttcgacct
    25441 gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga ttacgcttca
    25501 cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc atgctgtttt
    25561 ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt tgagggcatc
    25621 cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgaa tcggtacgcg ccagcaccac
    25681 gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc cctgcgcagg
    25741 ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc ggtcgatgtc
    25801 acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac gaaaaccttc
    25861 tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga tgacgtcctg
    25921 ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt ctgcaataaa
    25981 tgccttttgc ccggtgccgc agagaaaatc ggcgcggcta aaatccatcg aaaagaagcg
    26041 tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc cgcgcatttc
    26101 agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa agcgcgattt
    26161 cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca gcgtgcacag
    26221 ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt tatagacatt
    26281 cagctctttt gaggcgtgaa ccaacgtgcc gacgcgatag ttgtaccagg gatcgttcag
    26341 cccaaccgcc acctcaatag cgcaaatccc cgccacggtg cctgtctcgc tatcttccag
    26401 cacgaacaca tagccctgct cgcttttggg cagttcacct tgccaggttt tgattgccct
    26461 ttcgatacgc gccgaaagcg tggcttcatt ggcgggaagc gacgtcaggc cgccgcccgt
    26521 tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac ggatgaccat
    26581 catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc cagtccggtc
    26641 gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt gccacccgca
    26701 atcagtacca tcacgcctgc tttcgccgct tccagagaga tctgtttcgc ttgcccggcg
    26761 taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc actgaacaaa
    26821 ccatagcggt gattaatagt attaagacgc tcaacaaacc agtcgtgacg ctgtttaacg
    26881 ccattaagca tctctggtgt gttgatgagc tccagcactt tgcctgctac cgccgaggcc
    26941 agcggattac cgccataggt ggtgccatga gtgccaacgg tcatcacgcg ggcgcactct
    27001 tcggttgcca acaacgcacc gaccgggaaa ccgccgccca gcgctttggc ggtggttaac
    27061 agatcgggcg tcacgccgta gtgcatatag gcatacagct caccagtgcg cccgacgccg
    27121 gtttgtacct catcaaaaat cagcaaggca ttgtggcggt cacacaattc acgcagacct
    27181 tgtaaaaacg cgttgctggc tggcaccaca ccgccttcgc cctagatggg ctcgacaatc
    27241 accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt atatgcagca
    27301 tgacgaatat ccgccggcag tggcgcaaaa tcctgtgaat aggctggctg cccacccgca
    27361 ctgacagtaa acagcgtgcg accatgaaac gcatttttga acgccacgat gccactctta
    27421 tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc ttcgttggct
    27481 tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat caattttttc
    27541 gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa cttactcgcc
    27601 tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt caccgcaatg
    27661 ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga accttcgcca
    27721 cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc aaagttttca
    27781 cgcgtaattg gctgagacat agcgacctct acagtaaata attcgttatt tgcatgttaa
    27841 caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc cagtgataaa
    27901 agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac catatgcatt
    27961 taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa taaaaaacgc
    28021 aatgttatgc agaagtaaaa tataattcta gaattgtgat cattgacgaa atttactgga
    28081 aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg cgccatcatt
    28141 gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca acgcgaaatt
    28201 ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa atttgtctct
    28261 tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt cgaaaagcac
    28321 caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt tccgctcgaa
    28381 gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca ttatggcgtg
    28441 gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg tgacgacgaa
    28501 gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa tgtcaccgtg
    28561 atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt cccggcaaaa
    28621 gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg tgataatccg
    28681 gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg cattggcgaa
    28741 gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga agagcgcgaa
    28801 tggatggaca ggctgatgag ctggggggtg gtcgatacct tccgccatgc taatccgcaa
    28861 acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga taaccgtggt
    28921 ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt agaaaccggc
    28981 atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt ctgggcgacc
    29041 ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat taaccttgag
    29101 aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta taccgtcact
    29161 gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa actccacgat
    29221 gaacgctgag cgtaactttc tgtttgcctg tcttattttt gcgctggtca tttacgctat
    29281 ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga cactcattcg
    29341 ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcaatg ccccctcttt
    29401 ctgttaccag gaagcatact ggtgatcgct ggaggaatag tttttggccc gctcttagga
    29461 cacaactctc attaattgcc gcaacgctgg cctcctcgtg ctcattcctg ctggcgcgct
    29521 ggctgggacg tgatttactg ctgaaatacg tgggccatag ccataccttc caggccattg
    29581 aaaaaggcat tgcgcgtaac ggtattgatt ttcttattct gacccgctta atcccgttgt
    29641 ttccttacaa tattcaaaat tacgcttacg gattcaccac catcgccttc tggccttata
    29701 cccttatttc ggcactcacg accctgcccg gtattgttat ttataccgtg atggcaagcg
    29761 atctcgccaa tgaaggcatt acgctgcgct ttattttaca actctgtctg gcgggcctgg
    29821 cgctgtttat tctcatccag ctcgcaaaac tctacgcccg acacaaacat gtggatctgt
    29881 ctgcttcgcg ccgcagccca cttactcacc caaaaaatga aggatagaac gatgtcgcaa
    29941 cattattcag tgtcatggaa aaaaggactg gctgcactct gcttactggc tgttgccggg
    30001 cttagcggct gcgatcaaaa agagaatgcg gcggcaaaag tggaatacga cggactttcg
    30061 aacagccaac cgctgcgcgt cgatgccaat aaccatacgg taaccatgct ggtgcaaatt
    30121 aatggtcgtt tcctcaccga cgacactcgt cacggtattg tgtttaaaga tggctccaac
    30181 ggacataaat cgctgtttat ggcttatgcg cccccgaaag cattttatga agccctgaaa
    30241 gaggcaggtg gtacgccggg cgaaaacatg acgatggata ataaagaaac gactcatgtc
    30301 acaggcagca aactggatat ttcggtcaac tggcaagggg cggcaaaagc gtattccttc
    30361 gatgaagtga ttgttgacag taatggcaag aaactggaca tgcgctttgg cggtaattta
    30421 acggcagcag aagagaagaa aacgggttgc ctggtgtgtc tggatagctg cccggtcggc
    30481 atcgtcagca atgcaacata cacttatggt gcggtggaaa aacacggcga agtgaaattc
    30541 aaaggcaatg cctcagttct cccggcggat aacacgctgg caacggttac ctttaaaatc
    30601 accgaataaa gccaagataa aggatgatga agatacaatc gcgtaaaatc tggtactacc
    30661 gtataaccct catcatcctg ttattcgcta tgctgctcgc atgggcgctg cttcccggcg
    30721 tccatgagtt tatcaatcgc agcgttgcgg cgtttgccgc cgtggaccaa cagggtatag
    30781 aacgctttat tcagtcttac ggcgcactgg cggcagttgt ctcgttcttg ttgatgattt
    30841 tacaggccat tgctgcaccg ctacctgcgt ttttgatcac ctttgccaat gcgtcgctgt
    30901 ttggcgcgtt ctggggcggc ttgctgtcgt ggaccagttc gatggccggc gcggcgctgt
    30961 gcttttttat cgccagagtg atgggccgcg aagtggtgga aaaattaacc ggcaaaaccg
    31021 tgcttgacag tatggacggc tttttcactc gctacggcaa acacaccatc ctggtctgtc
    31081 ggttattgcc ttttgtccct ttcgatccaa tcagctatgc tgccggtctg acttcaatac
    31141 gttttcgctc gttttttatc gccaccgggc ttggtcaatt accggcgact attgtttatt
    31201 cctgggcagg cagcatgtta acaggcggta ctttctggtt tgtcaccgaa ctgtttattc
    31261 tgtttgccct gaccgtggtg atttttatgg cgaagaaaat atggcttgag cgccagaaga
    31321 ggaatgactg atgggtttac cgccgcttag caaaattcct ttcattttac gtccgcaggc
    31381 gtggctgcat cgtcgccatt acggcgaggt gctaagcccg attcgctggt gggggcggat
    31441 cccgtttatc ttttatttgg tgtcaatgtt tgttgggtgg ctggagcgca aacgctcacc
    31501 gctcgatccg gtagtgcgat cgcttgtcag cgcgcgcatt gcgcaaatgt gcctgtgtga
    31561 gttttgcgtg gatatcacca gtatgaaagt cgccgagcgt accggcagca ccgataaact
    31621 gctggcagtg gctgactggc ggcaaagccc gctctttagc gatgaagaac ggctggcgct
    31681 ggagtacgcc gaagccgcaa gcgtcacacc gccaacagtc gatgatgccc tgcgtacccg
    31741 actggctgcg cattttgacg ctcaggcgct caccgaactg acggcattga tcggcctgca
    31801 aaacctgtcg gctcgtttta attctgccat ggacattccc gctcaggggc tgtgccgtat
    31861 tccggaaaaa cgttcttaag gagggaagat gcgccattgt gggtggttgc tgggattgtt
    31921 atcgctgttt tctttggcaa cacatgccag tgactggcag gaaattaaaa atgaggccaa
    31981 agggcaaacc gtctggttta acgcctgggg cggcgatacc gcaattaacc gctatctcga
    32041 ctgggttagc ggcgagatga aaacccatta cgctataaac ctgaagattg ttcgtctcgc
    32101 ggatgccgca gacgcggtga agcgcattca gaccgaagct gctgccggtc gtaaaacggg
    32161 cggctcggtg gatctgctct gggtgaacgg cgaaaacttc cgcaccttaa a
//
LOCUS       DAEEGA010000043        31881 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEEGA010000043 DAEEGA010000000
VERSION     DAEEGA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31881
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.043"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(97..1473)
                     /gene="yeaG"
                     /locus_tag="KLH46_002139"
                     /pseudo
     CDS             complement(97..1473)
                     /gene="yeaG"
                     /locus_tag="KLH46_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
     gene            1909..2655
                     /gene="mipA"
                     /locus_tag="KLH46_002140"
     CDS             1909..2655
                     /gene="mipA"
                     /locus_tag="KLH46_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBE0378815.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2745..3506
                     /locus_tag="KLH46_002141"
     CDS             2745..3506
                     /locus_tag="KLH46_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBE0378816.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3557..4441)
                     /locus_tag="KLH46_002142"
     CDS             complement(3557..4441)
                     /locus_tag="KLH46_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBE0378817.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4525..5520)
                     /gene="gapA"
                     /locus_tag="KLH46_002143"
     CDS             complement(4525..5520)
                     /gene="gapA"
                     /locus_tag="KLH46_002143"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBE0378818.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5862..6275
                     /gene="msrB"
                     /locus_tag="KLH46_002144"
     CDS             5862..6275
                     /gene="msrB"
                     /locus_tag="KLH46_002144"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBE0378819.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6317..6589
                     /locus_tag="KLH46_002145"
     CDS             6317..6589
                     /locus_tag="KLH46_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBE0378820.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6959..8035
                     /locus_tag="KLH46_002146"
     CDS             6959..8035
                     /locus_tag="KLH46_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBE0378821.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDMVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            8062..9441
                     /locus_tag="KLH46_002147"
     CDS             8062..9441
                     /locus_tag="KLH46_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0378822.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9458..10503
                     /locus_tag="KLH46_002148"
                     /pseudo
     CDS             9458..10503
                     /locus_tag="KLH46_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10524..11360
                     /locus_tag="KLH46_002149"
     CDS             10524..11360
                     /locus_tag="KLH46_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBE0378823.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11365..12312
                     /locus_tag="KLH46_002150"
     CDS             11365..12312
                     /locus_tag="KLH46_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HBE0378824.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12322..13302
                     /gene="ydjG"
                     /locus_tag="KLH46_002151"
     CDS             12322..13302
                     /gene="ydjG"
                     /locus_tag="KLH46_002151"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HBE0378825.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13439..14197
                     /locus_tag="KLH46_002152"
     CDS             13439..14197
                     /locus_tag="KLH46_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE0378826.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14315..15673
                     /locus_tag="KLH46_002153"
     CDS             14315..15673
                     /locus_tag="KLH46_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0378827.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15766..16407)
                     /gene="pncA"
                     /locus_tag="KLH46_002154"
     CDS             complement(15766..16407)
                     /gene="pncA"
                     /locus_tag="KLH46_002154"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBE0378828.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16418..17434)
                     /gene="ansA"
                     /locus_tag="KLH46_002155"
     CDS             complement(16418..17434)
                     /gene="ansA"
                     /locus_tag="KLH46_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBE0378829.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17601..19457)
                     /gene="sppA"
                     /locus_tag="KLH46_002156"
     CDS             complement(17601..19457)
                     /gene="sppA"
                     /locus_tag="KLH46_002156"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBE0378830.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19618..20169
                     /locus_tag="KLH46_002157"
     CDS             19618..20169
                     /locus_tag="KLH46_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBE0378831.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20286..21329
                     /gene="selD"
                     /locus_tag="KLH46_002158"
     CDS             20286..21329
                     /gene="selD"
                     /locus_tag="KLH46_002158"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBE0378832.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDLNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21334..23295
                     /gene="topB"
                     /locus_tag="KLH46_002159"
     CDS             21334..23295
                     /gene="topB"
                     /locus_tag="KLH46_002159"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBE0378833.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23422..24462
                     /locus_tag="KLH46_002160"
     CDS             23422..24462
                     /locus_tag="KLH46_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBE0378834.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24579..25922)
                     /gene="gdhA"
                     /locus_tag="KLH46_002161"
     CDS             complement(24579..25922)
                     /gene="gdhA"
                     /locus_tag="KLH46_002161"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBE0378835.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            26158..26430
                     /locus_tag="KLH46_002162"
     CDS             26158..26430
                     /locus_tag="KLH46_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBE0378836.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(26396..26803)
                     /gene="nudG"
                     /locus_tag="KLH46_002163"
     CDS             complement(26396..26803)
                     /gene="nudG"
                     /locus_tag="KLH46_002163"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBE0378837.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            26890..27510
                     /locus_tag="KLH46_002164"
     CDS             26890..27510
                     /locus_tag="KLH46_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBE0378838.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27519..28826)
                     /gene="ynjE"
                     /locus_tag="KLH46_002165"
                     /pseudo
     CDS             complement(27519..28826)
                     /gene="ynjE"
                     /locus_tag="KLH46_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(28893..29546)
                     /locus_tag="KLH46_002166"
     CDS             complement(28893..29546)
                     /locus_tag="KLH46_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE0378839.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29546..31063)
                     /locus_tag="KLH46_002167"
                     /pseudo
     CDS             complement(29546..31063)
                     /locus_tag="KLH46_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(31036..31881)
                     /locus_tag="KLH46_002168"
                     /pseudo
     CDS             complement(31036..31881)
                     /locus_tag="KLH46_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7992 a         7635 c         8264 g         7990 t
ORIGIN      
        1 cgtcactctc gtctggggaa tatctccccg gcagccttca gggaaaaata tcatcagatg
       61 gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct caatctgctg
      121 ttccaggacg tagaacagat ggaccgggtt tgccgccact tctacatgat cgaagttgaa
      181 cacgcgggag aggatcttaa acgcaaaacg cgtcgacaga ccgttcatcc cttcatcgac
      241 accggcgtag tcacgatatt cctgatacga cttggctttc ggatcggtgt ctttcagact
      301 ttcgccatca taaacccgca tctttgaata aatgctggag ttttctggct ctttcaggcg
      361 cgaaagaatg gaaaaacgtg acagtgtttc gagcgtgcca ggggcgcatg gggcgtgagt
      421 caattcactg tgattaagca atttctcgta gattttgatc tcttcggaaa tgcgcaagca
      481 atacggcacc ttcacgatgt aaacacggtc gaggaaggct tcgttgtttt tgttattacg
      541 gaaagtgacc cattcggact tgttcgagtg ggcgagaata atcccgttga acggcagggc
      601 ggagatacct tccgtcccgt tgtagttacc ttcctgagtg gcggttaaca agggatgcag
      661 cacttttatc ggtgctttaa acatctcaac gaattccatg atcccctgat tggcgcggca
      721 cagcgcaccg gaatagccgt aggcgtccgg gtcattctgc gcgtagtgtt cgagtttacg
      781 aatatcgact ttcccaacca gcgcggagat gtcctggttg ttctcatcac cgggttccgt
      841 tttggcgata gcaatttgtt gcagaattga cggccagacc ttcactaccc ggaacttagt
      901 gatatcgcca ccaaattcat gcaggcgttt tgccgcccat ggcgacatga tggtgccgag
      961 ataacggcga ggaatgccat actctttttc cagaatctga gcatcttcct gcggattgaa
     1021 aagacagaac ggatgatcgt tgaccgggct acgctcaccg ttggcgctca atacataaat
     1081 cggtacgagc tgcattaatg atttcagtcg ctcagcaagc gatgatttac ccccacccac
     1141 aggccccagc agatacagga tttgtttctt ctcttccagc ccctgagccg cgtgtttcag
     1201 ataagagaca atctgttcaa tcgcgtcttc catgccgtaa aactcttcaa atgccggata
     1261 acgtgcaatg acccggttag aaaagagtcg agaaagtctg gggtcctggg ctgtatcgac
     1321 catgacaggc tcaccgatag ccatcaatag ccgctcagcc gcgttggcat aagcactgcg
     1381 atcttgccga caagtggtaa gaaactcctg cagtgtgaac tcttcgtcct tggcagcttc
     1441 atatcgctgg cgatagtgat caaatatatt catggtatgc cgtcctttcg ttttttagca
     1501 caggttaaga gccgttcata tgaatagtag aggctcccgg aagaggtaaa ctgaacgacg
     1561 tctcttgcta aaacacagca actctcatgc cattttatcg acgatgagaa caacgtgccc
     1621 ggtgatacac cttcaaaatt aagcgtagat ggcaaatgga aaacttgcct gcacggcttt
     1681 cttaatttca atggcatatc aacaactcat ccataaattt tgcataatta atgtaaagac
     1741 caggctcgcc agtaacgcta aatccatttg gctgtaagcg cggtgtcatc cgcggcagga
     1801 aaattaaaca gttactttaa aaaatgaaaa cgtaaaaagg ttgggtttcg atgtattgac
     1861 gggtaaactt tgtcgcccgc taaacatttg tttaaggaat gattaattgt gaccaaactc
     1921 aaacttctgg cacttggagt gcttatcgca acgtctgcag gcgtagcgca cgctgaaggt
     1981 aaattttccc tgggcgcagg cgtaggtgtc gttgagcacc catataaaga ttacgatacc
     2041 gatgtttacc cagtaccggt aatcaactat gaaggcgata acttctggtt ccgtggctta
     2101 ggtggtggtt actacctgtg gaatgacgca acggataaac tttcaattac cgcttactgg
     2161 tcgccgcttt acttcaaagc gaaagacagt ggcgatcacc aaatgcgtca cctggatgac
     2221 cgtaagagca ccatgatggc tggtctgtct tatgctcact ttacccagta cggttacctg
     2281 cgtaccaccc tggctggcga taccctggat aacagcaacg gcattgtctg ggatatggcc
     2341 tggttgtatc gttacaccaa cggtggcctg accgtgactc cgggtattgg tgtgcagtgg
     2401 aacagcgaaa accagaacga atactattat ggcgtatcgc gcaaagagtc cgctcgcagc
     2461 ggtctgcgtg gctataaccc gaatgacagc tggagccctt accttgagct gagcgccagc
     2521 tacaacttcc tcggcgactg gagtgtttac ggtaccgcgc gctacacccg tctgtctgat
     2581 gaagttactg acagcccgat ggtggataaa tcctggactg gcctgatttc taccgggatc
     2641 acctacaaat tctgataatg catctttcca gggcattttg tgcataaaaa cagggcgtca
     2701 cgccctgttt tgcattacgg tggtgcaata cctgggagga aacaatgcaa caaaaaatga
     2761 ttcaatttag tggcgatgtc tcactgccag ccatagggca gggaacatgg tatatgggcg
     2821 aagatgccag tcagcgcaaa acagaagttg ctgcactacg cgcaggcatt gaactcggtt
     2881 taaccctcat tgataccgcc gaaatgtatg ccgatggcgg tgccgaaaag gtggttgggg
     2941 aagcattaac cggtctgcga gagaaggtct ttctcgtctc taaagtctat ccgtggaatg
     3001 ctggcgggca aaaagcgata aatgcatgcg aagccagttt acgccgtctc aatactgact
     3061 atctcgatct ctacttatta cactggtctg gcagtttcgc ttttgaagag actgttgccg
     3121 cgatggaaaa attgatcgcc cagggaaaaa tccgtcgctg gggcgtttct aaccttggtt
     3181 atgccgatat gcaggaactc tggcagctgc cggggggaaa tcagtgtgcc actaatcagg
     3241 tgctttacca tctcggttca cgagggattg agtacgatct actcccctgg tgccagcaac
     3301 agcagatgcc ggtgatggct tacagtccat tagcccaggc cgggcggttg cgcaatggac
     3361 tgttaaaaaa cgcggtagtc aacgaaattg cacatgtcca acaacatgca gctgcgcttg
     3421 aggtcgaact ttcgtcagcg gaattagcta tgctggataa ggcatatccg gcaccaaaag
     3481 gaaaaactgc gctggatatg gtgtgatgtt gatacgtgcc ggatgcatcg cacccggcac
     3541 acaacattaa cgcaaattaa cgtttggcaa cgcgaatgga ttgcgccaga tgtgcaggtt
     3601 tctctttggt gactttttgc gtttctgaag cgtaagccgt ttctacacaa acaaaggtct
     3661 tgtagccatc gtccggcatg tcgcccatgc taactgaaag cgccggtccc gggttccagc
     3721 cgacaacgtt cagatgatgc tggtggccta cggcgataat acgattcagc gcttcatcat
     3781 taatcacgct gcaatcttgc ggattcagat acacgcgatc ggtacggtca gggaaggtct
     3841 gaataccgtc ggtcagtaca tcttctttcg cgtcattcac tttatcaatg aagcgatcgc
     3901 ccagcccact gacgcttacc ttagcgatat cacccacgtt aaagtaggta tgcagggcag
     3961 aggtggtttc aaattcgcca tgtgattcaa gatcgatttc acaggtttta cccacgcgga
     4021 aatgcgctaa cagcgtaaag tcgtgcggcc agaatttttt cgtctcttcg ctttgtgtca
     4081 attcaaaagt cagcgctacg ccatcagcat cttcatgatg tgatttcagc gtccacggca
     4141 ggttgcgggc aaaaccgtgc gcaggcagac cttgttgtgc cgccggacca aaccacggcc
     4201 agcaaaccgg tacgccaccg cgaatagcga cgccattttt gaacggtgtg ttgttgctca
     4261 accacagaac ttcttcttca cccgcaggtt tccacgagag aaggtgtgcg ccctgtaatg
     4321 caaaagaggc ttttacctgg ggatgatcga ccacaatgag gtccagttca tccagtttac
     4381 gacgggagag gacaggggag atttgttcga tgaccggaag ggcaaaaatt ttcttaatca
     4441 tgacgcagtc ctttaacttc attttatcag gtaaaaaaaa gagcgaccga agtcgctctt
     4501 tttagatcac agtgtcatct caacttattt ggagatgtga gcgatcaggt ccagaacttt
     4561 gttggagtaa ccggtttcgt tgtcgtacca ggataccagt ttcacgaagt tgtcgttcag
     4621 agcgatacca gctttagcat cgaacacgga agtgcaaact tcgccgttga aatcggtaga
     4681 tactacgtca tcttcggtgt agcccagaac gcctttcatt tcgccttcag cagcagcttt
     4741 aacggcagct ttgatctgct cgtaagttgc agctttttcc agacgaacgg tcaggtcaac
     4801 tacggatacg ttcggggtcg gaacgcggaa cgccatacca gtcagtttgc cattcagttc
     4861 tggcagtact ttacctacag ctttagcagc accggtagag gacgggatga tgttctggga
     4921 agcgccgcgg ccgccgcgcc agtctttgtg agacgggcca tcaacggttt tctgagtagc
     4981 ggtagtagcg tgaacggtgg tcatcagacc ttcgatgatg ccgaagttat cgttgataac
     5041 tttagccagc ggagctaggc agttggtggt gcaggaagcg ttggaaacga tgtcctggcc
     5101 agcatatttg tcgaagttag cgcctttaac gaacatcgga gtgttgtctt tagacggacc
     5161 agtcataacc actttcttcg caccagcggt gatgtgttta cgagcagttt cgtcagtcag
     5221 gaacagacca gttgcttcag cgacaacgtc aacacccact tcgtcccatt tcaggttagc
     5281 cggatcacgt tcagcggtaa cacggatttt tttaccgtta acgatcagat gaccgtcttt
     5341 cacttcaacg gtaccgtcga aacggccgtg agtggagtca tatttcagca tgtatgccat
     5401 gtaatcagcg tctaacaggt cgttgattgc aacgatctcg atgtcagaac gtttctgagc
     5461 agcacggaaa acaatgcgac cgatacggcc aaaaccgttg atacctactt tgatagtcat
     5521 atattccacc agctatttgt tagtgaataa aaggttgcct gtaaaattac aaaaacctta
     5581 cgcagcgtca agcggaatcg tgtcaatcat tgcgacaaat caatcctgtg cctaagcatt
     5641 acgcgactga ctcgcctcac ttttcctttg ggcttgagac cacatggggt cggcgccccg
     5701 aattttaaag ggcaattacg ataaaaatgt gattttcatc acgatttagg tgcagccatt
     5761 tcgtcgagat caagtttagg acaaaatttt gccagttggt gttaatgttt tgttagaatc
     5821 agtcacgtaa tgtgagcacg attaaagtga gatgtgagta aatggcgaat aaaccttcgg
     5881 cagaagaact gaaaaaaaat ttgtccgaga tgcagtttta cgtgacgcag aatcatggga
     5941 cagaaccgcc atttacgggt cgtttactgc ataacaagcg tgacggcgta tatcactgtt
     6001 tgatctgcga tgccccgctg tttcattccg aaaccaagta tgattccggc tgtggctggc
     6061 ccagtttcta cgaaccggtg agtgaagaat ccattcgtta tatcaaagac ttgtcacatg
     6121 gaatgcagcg catagaaatt cgttgcggta actgtgatgc ccatctgggg catgtctttc
     6181 ccgacgggcc gcaaccaaca ggcgaacgtt attgtgttaa ctctgcttct ttacgcttca
     6241 ctgatggcga aaacggcgaa gaaatcaacg gttgaaaaaa cgattcagca acttattcca
     6301 caggagcgga aaaattatga atcttgatga cattatcaac agcatgacgc ctgaggtata
     6361 ccagcgtttg tcgaccgccg ttgaactggg gaaatggcct gatggcgttg cgttgaccga
     6421 ggaacaaaaa gagaattgcc tgcaactggt gatgctgtgg caagcccgcc ataataccga
     6481 agcacagcat atgacaattg acacaaatgg tcagatggtc atgaagagca aacagcagtt
     6541 aaaagaagat tttggtatct cagcaaagcc tattgccatg tttaagtaag tggttcatcc
     6601 cctttttgcc gcggctatga aggggatagc caacaatgac cccacgactc gtctcaatta
     6661 tctcgacccg acctggctaa gtcccagtcc gatctcttgc agtcgcgttc acgcctgaat
     6721 gacttctcct gaacttaagt ctcagaccta tttggccggt aatccctctc gaatttgctc
     6781 tttttgcgtc ccgccttcca ccaacctgta acagacccag ccattttctg cgtaattgtg
     6841 atctgagttg taaaatgcag gtcattatct gtgttgtgca gatttaatat tgacctcaaa
     6901 aatcatgtgg gtaagtatga tggccatcta agcaaaacaa aacacagagg atgacgcaat
     6961 gaaagcactg gctcggtttg gcaaggcctt tggcggctac aagatgattc atgtcccaca
     7021 acccatttgt ggcccggaag acgtagtgat tgaaattaaa gccgcggcaa tctgcggcgc
     7081 agacatgaag cactacaatg tcgatagcgg ttctgatgag tttaactcta tccgcggcca
     7141 tgagttcgca ggttgtattg cgcaggttgg tgaaaaagtc aaagactgga aagtggggca
     7201 acgcgtcgtg tcggataaca gcggtcacgt ttgcggcgtt tgtccggcct gtgaacaggg
     7261 tgattttctg tgttgtacag aaaaagtaaa ccttggtctg gataacaata cctggggcgg
     7321 tggtttttcc aaatattgtc tggttcctgg tgaaattctc aaaattcatc gtcatgcgtt
     7381 gtgggaaatc cctgatggtg ttgattatga ggacgcagcc gtacttgacc ctatctgtaa
     7441 tgcctataaa tccatcgcgc agcaatcgaa attccttcct ggtcaggatg ttgtcgtcat
     7501 tggcactggc ccactcgggc tgttttccgt acaaatggcg cggattatgg gggcggtaaa
     7561 tatcgtcgtc gttggtctgc aagaagatgt ggcggtccgc ttcccggttg caaaagaact
     7621 gggggcgacg gcagtagtaa atggttctac cgaagatatg gtggcgcgct gccagcaaat
     7681 ttgtggcaaa gataatctgg gactggtgat tgaatgctcc ggtgccaata tcgcactgaa
     7741 acaagccatc gaaatgcttc gcccgaatgg ggaagtggta cgcgttggaa tgggcttcaa
     7801 acctcttgat ttctcgatta atgacattac cgcctggaat aaaagcatca ttgggcatat
     7861 ggcctatgac tccacctcat ggcgtaacgc tatccggcta ttagccagcg gcgctatcaa
     7921 agtcaaaccg atgatcacgc atcgtatcgg cctgtcgcaa tggcgcgaag ggtttgatgc
     7981 gatggtcgat aaaaccgcaa tcaaagtgat catgacttac gactttgatg aataactact
     8041 ctcctgaagg aacgttatcg catggaacag ataacaaaac cgcattgtgg tgcacggctg
     8101 gatcgcttac cggattgccg ctggcattcg tcaatgtttg ccatcgtcgc gtttggcttg
     8161 cttgtctgct ggagtaatgc cgttggtggc ttgatcctcg cgcagctgaa agcgttgggc
     8221 tggacagata attccactac agccacattc tcagcaatca cgaccgccgg aatgtttctc
     8281 ggtgcgctgg tgggcggcat cattggcgac aaaaccggtc gcagaaatgc gttcatcctc
     8341 tatgaggcca ttcatattgc ctcgatggtt gtcggtgctt tctcaccgaa tatggatttt
     8401 cttattgcct gccgttttgt gatggacgtt gggctgggag ctttactggt tacactgttt
     8461 gctggtttca ccgaatatat gcccggtaga aatcgcggaa cgtggtccag tcgggtttcc
     8521 tttattggca actggtcata tccgctctgc tcactgattg cgatgggact cacgccgctg
     8581 attagtgcag agtggaactg gcgggtacaa ctgcttattc ctgcaatatt gtcgcttatc
     8641 gctacggcgc tggcctggcg ctactttcct gaatccccgc gctggctgga atcgcgcgga
     8701 cggtatcagg aagccgagaa agtcatgcgg agtatagaag aaggcgtcat acgccagacg
     8761 ggtaaacctt tgccgcctgt ggttattgct gatgacggta aagcgccaca agcggtgccg
     8821 tattcagcct tactgacagg agtattactg aaacgcgtga tattaggctc ttgtgtgctg
     8881 attaccatga acgttgtgca gtacacacta attaactggt tgccaacaat attcatgacc
     8941 caggggatta atttaaaaga ctcgattgtt ttaaatacca tgagtatgtt tggcgcgcca
     9001 tttggtattt ttattgccat gctggtgatg gataaaattc cgcgtaaaac aatgggtgtg
     9061 gggctattaa tcctgattgc ggtgctcgga tatatctatt cactgcaaac cagtatgttg
     9121 ctcataacgc tgattggttt cttcctgatt actttcgtct atatgtacgt ttgctatgcc
     9181 tcggcagtgt atgtccctga aatctggccg acagaggcca aactccgtgg ctccggtctg
     9241 gcgaatgcgg tagggcgaat cagtggtatt gccgcacctt atgccgttgc agtgctgctc
     9301 agtagttatg gtgtaacggg agtctttatt cttctggggg ctgtttcaaa tattgtcgca
     9361 attgctatcg ccaccattgg aattgaaacc aaaggtgtct ccgttgaaag tttaagtatt
     9421 gatgcagtag ccaataaata atacgggtgg attcaaaatg aaaaattcaa aagcaatatt
     9481 gcaggtgccg ggcacaatga aaattatttc agcagaaata ccagtgccta aagaagatga
     9541 agttttgatt aaagtagaat atgtcggtat ttgtggttca gatgtacatg gttttgaatc
     9601 aggcccgttt attccgccta aagacccaaa tcaagaaatt ggcctgggtc atgaatgcgc
     9661 cgggacggtt gtggctgtgg gaagccgcgt gcgcaaattt aaaccggggg atcgggtaaa
     9721 tatcgaacct ggcgttcctt gcggtcactg tcgttactgt ctggaaggca aatataacat
     9781 ctgcccggac gttgatttta tggcgacaca acccaactac cgcggcgcat taacgcacta
     9841 tctgtgtcat ccggagagct ttacttacaa actgcccgac aatatggaca cgatggaagg
     9901 ggcgctggtg gagcctgccg cagtcgggat gcatgccgcg atgctggcag atgttaaacc
     9961 gggtaagaag ataattattc tgggagcagg ttgtattggt ttgatgacgt tgcaagcgtg
    10021 caaatgcctg ggcgcaacgg aaattgccgt cgttgatgta ctggaaaaac gtctggcaat
    10081 gggggaacag cctggtgcga cagtggttat taatggcgca aaagaggaca ctattgcacg
    10141 ctgtcagcaa tttaccgaag acatgggcgc agatattgtt ttcgaaacag cgggttctgc
    10201 ggtcaccgtt aaacaggcac ctttaatctg gtaatgcgcg gcggtaaaat tatgattgtt
    10261 ggtactgtac ccggcgattc ggcaatcaat ttcctcaaaa tcaatcgcga agtcactatc
    10321 cagacggtat tccgctatgc caatcgttat ccggtcacga ttgaagctat ttcttcaggg
    10381 cgattcgatg tgaaatcgat ggtgacgcat atttacgatt atcgggatgt acaacaggca
    10441 tttgaagagt cagttaacaa caaacgcgac attattaaag gcgttattaa aattagcgat
    10501 taattaatac gggagaacag aacatgctcg cagatatcag atattgggaa aacgatgcca
    10561 caaataaata ttatgcaatt gcccatttta acgtatggaa tgcagaaatg ttgatgggcg
    10621 ttaaagacgc agccgaagaa gcgaaatccc cggttattat ttcttttagt acaggttttg
    10681 tcggtaacac ctcatttgaa gatttctctc acatgatggt atcaatggca caaaaagcaa
    10741 cggtgccggt aataactcat tgggatcatg gtcggagtat tgagattatt cataacgcct
    10801 ggactcatgg tatgaattca ttaatgcgtg atgcttctgc atttgatttc gaagaaaata
    10861 ttcgtttaac caaagaggct gttgatttct ttcatccgct gggtattccg gtagaggcgg
    10921 aattagggca tgtcggtaat gaaaccgttt atgaggaagc gctggcgggc tatcactata
    10981 ccgatcctga ccaggcggct gaatttgttg aacgtacggg ctgtgactca ttggctgtcg
    11041 ccatcggcaa ccagcatggg gtttatacgt cagagccgca attgaacttt gaggtcgtca
    11101 aacgcgtacg cgatgctgtt tctgttccgc tggttttgca tggcgcatcg gggatcagtg
    11161 atgccgacat taaaactgca atttcgttgg gtattgcgaa aatcaacatt catacggagc
    11221 tctgtcaggc cgcaatggta gcagtgaaag aaaaccagga tcagcctttc ctgcatttag
    11281 aacgtgaagt acgtaaagct gtaaaagaac gggcattgga aaaaattgaa ctgttcggtt
    11341 ctgatggcaa agcggaataa tgatatggat aatctcgacg ttatttgtat aggtgccgct
    11401 attgttgata ttccattaca accggtcagt aaaaatatct ttgatgtaga ttcttatcct
    11461 cttgaaagaa tcgcaatgag caccgggggc gatgcaataa atgaagcaac aattatttct
    11521 cgtctggggc atcgtaccgc attaatgagc cgcattggta aagatgcctc agggcaattt
    11581 attctcgacc attgtcgcaa agagaatatt gatattcaaa gcctgaaaca ggacgtcagc
    11641 atagatacat ctattaacgt tggactggtg acggaggatg gcgagcggac gtttgtcacg
    11701 aaccgtaacg gcagtttgtg gaaattaaat attgacgatg ttgattttgc gcggtttcct
    11761 caggcgaaat tattatcact ggccagtatt ttcaacagtc ctctattgga tggtaaagcg
    11821 ctaacagaaa tttttacgca agcgaaagcc cggcagatga tcatttgtgc cgatatgatc
    11881 aaaccgcggt tgaatgaaac gctggatgat atttgcgaag cattgagtta tgtcgattat
    11941 ctgtttccaa attttgccga ggaaaaatta ctcacaggga aagagacact ggatgaaatt
    12001 gctgactgct ttcttgcgtg cggcgtaaaa acggtggtga ttaaaacggg taaagacggc
    12061 tgctttatca agcgtggtga catgacgatg aaggtgccgg cggtcgcagg aataaccgcc
    12121 atcgacacca ttggcgcggg cgataacttt gcttcaggtt ttattgcggc actgttagaa
    12181 ggcaaaaatc tgcgtgaatg cgcacgcttt gccaatgcaa cggcggctat ctcggttcta
    12241 agcgtcggtg ccaccaccgg cgtaaaaaac agaaagctgg tcgaacaatt gctggaagaa
    12301 tacgaaggat aatgaaggca aatgaaaaag atacctttag gcacgacgga tataacgctt
    12361 tcgcgaatgg ggttggggac atgggccatt ggcggcggtc ctgcatggaa tggcgatctc
    12421 gatcggcaaa tatgtattga tacgattctt gaagcccatc gttgtggcat taatctgatt
    12481 gatactgcgc taggatataa ctttggcaat agtgaagtta tcgtcggtca ggcgttaaaa
    12541 aaactgcccc gtgaacaggt tgtagtagaa accaaatgcg gcattgtctg ggaacgaaaa
    12601 ggaagtttat tcaacaaagt tggcgatcgg cagttgtata aaaacctttc cccggaatct
    12661 atccgcgaag aggtagaagc cagcttgcaa cgtctgggta ttgattacat cgatatctat
    12721 atgacgcact ggcagtcggt gccgccattt tttacgccga tagctgaaac tgtcgcagtg
    12781 cttaatgagt taaaggctga agggaaaatt cgcgctatag gcgctgctaa cgtcgatgct
    12841 gaccatatcc gcgagtatct gcaatatggt gaactggata ttattcaggc gaaatacagt
    12901 attctcgacc gggcaatgga aaacgaactg ctgccgctat gtcgtgataa tggcattgtg
    12961 gttcaggttt attccccgct agagcaggga ttgttgaccg gcaccatcac tcgtgattac
    13021 gttccgggcg gcgctcgggc aaataaagtc tggttccagc gtgaaaacat gctgaaagtg
    13081 attgatatgc ttgaacagtg gcagccactt tgtgctcgtt atcagtgcac aattcccact
    13141 ctggcactgg cgtggatatt aaaacagagt gatttaatct ccattcttag tggggctact
    13201 gcaccggaac aggtacgcga aaatgtcgcg gcactgaata tcaacttatc ggatgcagac
    13261 gcaacattga tgagggaaat ggcagaggcc ctggagcgtt aaatactcaa cacgattcac
    13321 ctctgtttct gtgaaaactg tgatgaaaat ctgtgattca aataggttat tttgaaagta
    13381 aacatcggtt atgcgataat cgcgctaatg tgatgtaaaa agatacaggg gtgtaatcgt
    13441 ggcggcaaaa gacaggattc aggcaattaa gcaaatggtt gccaacgata aaaaggtgac
    13501 agtctcaaat ttgagtggga tttttcaggt aaccgaagaa accattcgcc gcgatcttga
    13561 gaagctggaa gatgaaggct ttttgaccag aacctatggt ggtgctgttt taaatacagc
    13621 ggtgttgacg gagaatatcc atttttataa gcgcgcttca tcgttttatg aagagaagct
    13681 gcttattgca cgcaaggcac taccctttat cgacaataaa accaccatgg cagccgattc
    13741 cagtagtacc gtaatggaat tgctcaaatt gttacaggac cgtagtgacc tgacgttgct
    13801 aaccaactcg gcggaagcga ttcatgttct ggctcagtca gaaattaaag tcgtttcaac
    13861 gggtggggaa ctaaacaaaa atacactttc cctgcaagga agaattacta aagagatcat
    13921 cagccgctac catgtcgata tcatggtaat gagttgtaaa ggtcttgata ttaacagcgg
    13981 cgcgctggac tctaatgaag cggaagctga aatcaaaaag acaatgatcc gtcaggcgac
    14041 agaagttgcg ttattggttg atcactctaa gtttgatcgt aaagcttttg tccagttagc
    14101 tgactttagt catattaatt atataataac tgataaatca ccgggtgcag agtggatagc
    14161 attttgcaaa gacaataata tccagctggt gtggtaattt aagcacacgt tgtcaatatt
    14221 atttgttcct gcgactggaa ataaaatgat gataaagatg cggcaagtga atttgccgca
    14281 attgctacct tttatagcaa atccgaggca cccaatggaa caatatgatc aaattggcgc
    14341 aagactggac cgcttgcctt tggcccggtt tcattatcgt atatttggta taataagctt
    14401 tagtctgtta ttaacggggt ttttgagtta ctcaggtaat gtcgtcttag caaagctggt
    14461 aagcaatgga tggtcaaata atttcctcta tgccgccttt acctcggcat taatgtttgg
    14521 ttatttcatc ggctcactta ctggtgggtt tattggtgac tactttgggc ggcgcagggc
    14581 gtttcgcata aatcttctca tcgtcggtat tgctgcaaca ggggccgctt ttgtccctga
    14641 tatgtactgg ctcattttct ttcgcttcct gatgggaaca ggaatggggg cgctgattat
    14701 ggttggctat gcctcattta cggagtttat ccccgcgacg gtgcgtggaa aatggtccgc
    14761 gcggctctca tttgttggta actggtcgcc catgctgtct gcggcgatag gcgtggtggt
    14821 tatcgctttt tttagttggc gaataatgtt tcttctgggt ggtattggca tactgttagc
    14881 ctggtttctc tcaggtaaat actttatcga gtcgccacga tggctggaag ggaaagggca
    14941 aatcgccggt gcagaaagcc aacttcgtga agtagagcag caaattgaaa gggagaagag
    15001 tattcgttta ccccagctta ctttgaacca gagcaacagc aaggttaagg taatcaaggg
    15061 taccttcagg ctcctgttta aaggggaaat gttacgacgt acattagttg cgataactgt
    15121 tttaattgca atgaacattt cgctttatac cattaccgta tggataccga ccatatttgt
    15181 taactccggc attgatgtcg ataaatcaat attaatgacc gctgttatta tgattggtgc
    15241 tccggtagga atatttattg cagcattaat tattgatcat tttcctcgtc gattatttgg
    15301 ctccgcctta cttattatta ttgccgtgtt aggctatatc tattcaagtc agactacaga
    15361 gtgggcgatt ttaatctatg gtctggtgat gatcttcttt ttatacatgt atgtttgctt
    15421 cgcgtcggcg gtttatatcc cggagctttg gccaacgcat ttacgcctgc gcggttcggg
    15481 tttcgttaat gccgtcggac ggatcgtcgc agttttcacg ccctatggcg ttgcggcatt
    15541 attaacacat tatgggtcga tcacggtgtt tatggtgctt ggtgtcatgt tagtgctctg
    15601 tgcgctggtt ctctccattt ttggcatcga aacgcggaag gtgtcgctgg aagagatttc
    15661 tgaggtgaat taaatctttc gttgatggca tcaattgcca gatgtaccac tgacgtgttt
    15721 tatttggcct acaatgccag tacagaattg taggccagcg taaaattacc cctgcgtctc
    15781 ttcccagtct gccagcgtat atagcgttgc cccagctgct gacatctcca taaacgcgtg
    15841 tgcactgtcc tggggctgga tattcacgcc acgacagcca tcggtaatca cgtttacctt
    15901 ataacctaac tgtaacgcgt ccagcacggt aaacttcacg caatagtcag tagccaggcc
    15961 cataacgatc aattcatcga tttcatgatc gcgcaaccag tcatcaagcg cggttttctg
    16021 ccgacgtccg ttatcaaaaa aggcactgta actgtcaact aaaggatttt cgcctttatg
    16081 gaacaccgct gcgatctctt tttgattcag taacggatgt aattgtgcgc cttcactgtt
    16141 ctgcacacag tgttcaggcc agaaagtttg ccgcaggccg ccgagttggc ctggcgtata
    16201 aggctctacg ccgtgctggc tggcaaagct gccgtgattc gccggatgcc agtcctggct
    16261 ggcgataacc gcttctccgc gcgactggca ccagtcaata aggcggttag caacctccac
    16321 cgtactgtca ccttccggta cggcgagcgc gccaccagca cagaaatcat tttgtaaatc
    16381 gaccagtaac agggcgcgat ggggcattgc agtctcctta atcatccggc gtcagttcgc
    16441 cgcgcaggtt ttggctcatg gccttgcgaa tggtttcagt atccagttcc tggctcagca
    16501 ggtaatgcag tttggttagc gtggcttcta cagtcatatc tgcaccgtca ataacgccgg
    16561 catgggcgag ggcgttaccg gtggcataac cactcatatt cactttaccg gacatacatt
    16621 gtgtcaggtt gaccaccaca ataccgcgat cgctggcttc ttgtaattcc tgcaggaagg
    16681 ctttgttttg tggcgcatta cccacgccat aggagcgcag aatcaatgct ttcaccggtt
    16741 ggcgcagaaa attgcgcacg acgtcagcag aaatccctgg ataaatcgtc accacgccaa
    16801 ttggttgtgg cgtgattgga tgaacgatta attccccttc accgtgcggg gcgggtggcg
    16861 tattcaaacg acgaatatgg ataccagctt ccagtaacgg aggaaggttt ggagaggcaa
    16921 acgcatcaaa accatcggca tgggctttgg tagtgcggtt gccgcgatac aatcggttat
    16981 tgaaaaagag cgttacttcg ttgatcggat aattcgccgc aacgtacaac gcattcagca
    17041 gattaatttg tccgtcagag cgtaactcag ccagcgggat ttgtgaccct gtcacaatga
    17101 ccggtttacc gagattctcg agcatgaacg acagcgcaga ggcggtatac gccatcgtgt
    17161 cggtaccgtg tagaatgaca aaaccatcat agtcgtcata gtgcgcttta atatcttcag
    17221 caatatgctg ccagtcttcc ggcgtcatat cagaagaatc catcaacggc gtatattcat
    17281 gaatggtgaa atctggcatc tccgggcgat ggaattccgg catcagcgcc agttggcgtt
    17341 gtagatgacc tgacaccgga atataaccct gctcggaacg ctgcatcccg atggtcccgc
    17401 ccgtgtaggc gacgtaaatt gatttctttt gcatgatatt gatatgaatg atatcgaaag
    17461 agcaaaagta tatacgtgag gcaggaggag cgcacctgat actatgcgga taaggaaata
    17521 tggggcgaga cagcgggaag aggattcgtc gaggtaaaaa ataatgtggc cgacgatctg
    17581 tcggccactc agtacaagac ttaacgcacg ttggcgcagg tcaggcaaaa cgcataacgg
    17641 ttttgtgggt cgttaaacgc ggccagcttg tcgctttcac ttttaacggt agaggctacc
    17701 gaggcaagcg gtgcaggtaa catggcctgg aacgcatctg gcaacattgc ccggacagaa
    17761 ccagacatgt tgtccatcac tttgtcgaag aaggtcggtt catcaacgta gtattccaga
    17821 tgccactgtt tcacttttgc gagctctgct gctttggcaa ccgcatcatc gaaatccccg
    17881 agactatcga ccagcccgtt agcttttgca tcctgaccgg tccagacgtg gccctgggcg
    17941 attttatcaa tttgctccgg cgtcgaatga cgcgcatcag caaccagcgt gataaagcgt
    18001 ttatagccat tctcaatgct taattgcatc atctgctgcg cttccggcgg cagtgccctg
    18061 gtgatagaaa catccgccag cggtgaagtt gagacaccat cagtatgaac accaatcgaa
    18121 tccagactat tttctacggt ggtgatcacg ccgaagatac cgatagaacc ggtcagggtg
    18181 ctggggttag ccacaatgta attagctggc gtggaaatcc agtaaccacc agatgccgcc
    18241 atgccgccca tcgatacaac cacagactta cccgctgccc gggctgctgc cagttcagcg
    18301 cgaatcactt cagacgcggt aacgctgccg cctgggctat taacacgcag gacaatcgct
    18361 ttcactttcg ggtcaaggcg agcgtcgcgg atttgtgccg cagtggtatc accgccaaca
    18421 ttcccctgag tttcctcgcc atccataatt gcgccattag cgaagacgac accgatgctg
    18481 tcaccggtat ctgccggcgt tttcaatgcg taatcgtaat aactgatggc gcgataattt
    18541 ttatcagtct tactccagcc gaactctttg gtcagagctt tttcgatttc ggcactcgat
    18601 gccagtgcat cgaccagctt gttttccagt gcatatttcg cggtatcgcc accggttttg
    18661 gttaaaccct caagcaaccc ttgcgcgcca gggaatacct gctgagcagg gatctgccgg
    18721 ttagcggcaa cagtattcag atagttttgc cacagctcac caatccagcg gctgtcagct
    18781 tcgcgggctg ccggtgacat atcatcacga ataaacggtt caacggcaga tttatacgta
    18841 cccacgcgga acacatgggt ggaaactttc agcttatcca gcaacgattt gtagtacaga
    18901 ccgttggtag caaaaccgtg cagatcaacc acgccttgcg gtgacagcca aattttattg
    18961 gcgaaactgg cgagataata ttgcccctgg ctgtagttct cgccaacggc ataaaccggt
    19021 ttcccgctgt cacgaaactc tttcagggct ttgccgatgt actgcataga cggctggtcg
    19081 ccgcctgcga agtttttcag atccatcacg ataccggtga tattgcggtc gtccttcgcc
    19141 tggcgaatgg tgttgacgat atcaaacagt gagttttcct gcagacgatc ggaactggca
    19201 ccaagcagct ggcggcttaa tttactaaac cgctgagaac tgtctggttt atcgacgatc
    19261 acaccagaaa tgtccagcag cagtgcgcca cgactggccg tttctttcga atcaccacca
    19321 ctgacctgca tccagatccc cacaccaacc agtacgagga aaataaagaa caggttaagt
    19381 accatttcac ggacgaaatt cagcagacgc cacgtccatt taaaaaatcc ggcaataaat
    19441 cgccaaaggg ttcgcatgta ttctcccaac ttaaggtcac acctgtcaca ggcgcaatag
    19501 atgtggttat cgtaatgacc caaccggcac ttgtcagcag gaattgcgga ctgtgctgta
    19561 acaaaatcca ctctcgtgtt aattttgtga ataaatatca cgacaggagt taatcaaatg
    19621 gatgcactcg aactattgat caatcgccgt agcgcctccc gcctggctga acccgcgcca
    19681 acgggtgaac aactgcaaaa catcctgcgt gcgggtatgc gtgcgccgga ccataagtcc
    19741 atgcaaccgt ggcatttttt tgtgattgaa ggggaagggt gcgagcgttt cagcgccgta
    19801 ctggaacagg gggcgattgc tgccggtagt gatgataaag ctatcgacaa agcccgtaat
    19861 gcgccgttcc gcgcaccgct catcatcacg gtggtggcga aatgcaaaga aaatcataaa
    19921 gtcccgcgat gggaacagga aatgtctgcc ggatgcgcgg tcatggcgat gcaaatggca
    19981 gccgttgccc agggatttgg cggcatctgg cgcagtggcg cattaactga aagtccggta
    20041 gtgcgtgaag cattcggttg ccgtgagcag gataaaattg tcggttttct ctacctcggc
    20101 acgccgcagt tgaaagcatc tacgtcgatt aacgtcccgg acccgacgcc gtttgtaact
    20161 tatttctgat aattgtcgcg aaactgtctg gattgtgagc aaaggcgcag gaattcagac
    20221 actctcactt atcacttcac ggaatgaggg ttaccatacc cggattgcat tgacaggaga
    20281 tgtccatgag cgagaactcg attcgtttga cccaatacag ccacggagct ggttgcggct
    20341 gtaaaatttc cccaaaagtg ttggaaacca tcctgcacag tgagcaggcg aagtttgttg
    20401 atctgaattt gcttgtgggt aatgaaaccc gcgacgatgc ggcggtgtac gatctgggca
    20461 atggcaccag cgttatcagt accaccgact tctttatgcc gatcgttgat aatcctttcg
    20521 attttggccg cattgcggcg actaacgcca tcagcgatat cttcgcgatg gggggcaaac
    20581 cgattatggc gattgcgatc ctcggctggc cgattaacaa actttccccg gaaattgccc
    20641 gcgaagtaac cgaaggtgga cgctatgcat gccgtcaggc gggtattgcg ctggctggcg
    20701 gtcactccat cgatgcgccg gagccgattt ttggtctggc ggtaacgggg atcgtaccga
    20761 ccgagcgggt gaagaaaaac agtaccgcac aagccggatg caaactgttc ctgacgaaac
    20821 cgctggggat cggcgttctt accacggctg agaaaaaatc actgttgaaa ccagaacatc
    20881 agggactggc gacggaagtg atgtgccgga tgaacatcgc aggcgcgtcc tttgccaaca
    20941 tcgaaggcgt aaaagcgatg accgacgtta cgggctttgg tctgctgggc cacttgagcg
    21001 aaatgtgtca gggggctggt gtgcaggcac gcgtcgacta tgaagcgatc ccgaaactcc
    21061 ccggtgttga agagtacatt aagttgggcg cagtacctgg cggcactgaa cgtaactttg
    21121 ccagctacgg tcatctgatg ggtgaaatgc cgcgtgaagt gcgcgatctg ctgtgcgatc
    21181 cgcaaacttc tggcggtttg ctgctggcgg tcatgccgga agcagaaaat gaggttaaaa
    21241 ctacagccgc cgagtttggc attgaactga cggcgattgg cgaactggtg ccagcgcgcg
    21301 gcggtcgtgc catggttgag atccgttaat ttaatgcggt tgtttattgc cgaaaaaccg
    21361 agtctggcgc gcgccattgc tgatgtcctg cccaaaccgc accggaaagg cgatggcttt
    21421 atcgagtgcg gtaatggtca ggtggtgact tggtgtatcg gtcacctgct tgagcaggcg
    21481 cagccagacg cctacgacag ccgctatgcg cgctggaatc ttgcggattt gccgattgtc
    21541 ccggaaaagt ggcaattaca gccacgaccc tccgtgacca aacaacttaa cgtcatcaaa
    21601 cgcttcctgc atgaagccag cgaaatcgtt cacgccgggg acccggatcg tgaagggcaa
    21661 ttgctggtgg atgaagtgct ggactatctg caactggcac cggaaaagcg ccagcaggtg
    21721 cagcgttgct tgataaacga cctgaacccg caggcggttg agcgggcgat cgaccgtctt
    21781 cgttccaaca gtgagtttgt gccattgtgc gtttctgcgc tggcgcgagc gcgtgccgac
    21841 tggctgtacg gcatcaatat gacccgtgcg tataccattc tcggtcgcaa tgccggttat
    21901 cagggcgtac tttccgtggg acgcgtgcag acacccgtgc tcgggctggt ggtgcgccgc
    21961 gatgaagaga ttgaaaactt cgtggcgaaa gacttctttg aagtcaaagc acatatcgtg
    22021 acacctgccg atgagcggtt taccgctatc tggcaaccga gcgaagcgtg tgaaccgtac
    22081 caggatgaag aagggcgctt gttacatcgt ccactggcgg agcatgtggt taaccgcatt
    22141 agtggtcaac cggctattgt caccagctat aacgataaac gggaatcaga atccgcgccg
    22201 ctgccgtttt cgctttcggc gttgcagatt gaagcggcaa aacgcttcgg tctgagcgcg
    22261 cagaacgtgc ttgatatctg ccagaagctg tacgaaacgc acaagctaat cacttatccg
    22321 cgttccgatt gtcgctattt gccagaagaa cattttgccg gacgccacgc ggtgatgaat
    22381 gccatcagcg ttcatgcacc ggatctgttg ccgcagccag tggtagatcc agatatacgc
    22441 aaccgctgtt gggatgacaa aaaggtcgat gcgcaccacg ccatcatccc gaccgcacgg
    22501 agttctgcga tcaacctgac ggagaacgaa gcgaaggtct ataacctgat tgcccgtcaa
    22561 tacctgatgc agttctgccc ggatgcggtg ttccgcaagt gtgttatcga actggacatt
    22621 gccaaaggca aatttgtcgc taaagcccgt tttcttgctg aagcaggctg gcgcacgctg
    22681 ttaggcagca aagagcgcga tgaagaaaac gacggcacgc cattgcctgt ggtggcgaaa
    22741 ggcgatgagt tgctgtgtga aaaaggtgaa gtggtagagc ggcaaaccca gccgccgcgc
    22801 cattttaccg atgcaacact gctttcggcg atgaccggga tcgcgcgctt tgtgcaggac
    22861 aaagatctga aaaaggtcct tcgtgcgacc gatggtctgg ggacagaagc aacccgtgcc
    22921 gggattattg aactgctgtt caagcgtggt tttctgacca aaaaagggcg ctatatccac
    22981 tccaccgacg ccggaaaagc gttattccat tcgctgccag aaatggcgac gcgaccggac
    23041 atgaccgcgc actgggaatc ggtgctgacg caaatcagcg aaaagcagtg tcgctatcag
    23101 gactttatgc agccgctggt ggggacgcta tatcagctta ttgatcaagc caaacgtacg
    23161 ccggtgcggc agtttcgcgg cattgtggct ccgggcagtg gtggcagtgc tgataagaaa
    23221 aaggctgcac cgcgtaaacg tagtgcgaaa aaaagtccgc cagcagatga agccggaagc
    23281 ggggcgatag cgtaagcgag cgaatctttc gtgctattcg agtcatattc tgaaatatcc
    23341 agcggatcaa gaaaattcgt tggatatttt tttgcatgga taaaattatc gcctctaaag
    23401 tatgtaataa cagggaatgt gatgaaaaaa gttttattgc aaaaccatcc tgggagcgag
    23461 aagtattctt ttaatggctg gggaatattt aatagtaatt ttgaacgggt gattaaagaa
    23521 aataaggcca tgctgctttg taagtggggg ttttatttaa catgtgttgt cgctgtaatg
    23581 tttgtattcg cagcgataac atccaacggt ttgaatgaaa gaggcctgat taccgcggga
    23641 tgctcttttc tttatctatt aattatgatg gggcttattg ttcgggccgg ttttaaagca
    23701 aaaaaagaac aactgcatta ttatcaggct aaaggtattg agccgctcag tatcgagaag
    23761 ttacaggcgc tacaattgat cgcaccttat cgattctatc ataagcaatg gtctgaaacg
    23821 ctagagttct ggccgcgaaa gcctgaacct ggcaaagata ccttccaata tcatgtgctt
    23881 ccttttgact cgatcgatat catcagtaaa agacgcgagt ctttagagaa tcaatggggt
    23941 atcgaagata gcgaaagtta ttgtgcctta atggagcatt ttctttctgg cgaccatgga
    24001 gccaatacct ttaaagcaaa catggaggaa gccccagagc aggttatcgc cttgttgaat
    24061 aaatttgctg tttttccctc agactatatc tctgattgcg ctaataatcg ctccggtaaa
    24121 tcctcggcga agctaatatg ggcggcggaa ttatcatgga tgatctcgat atcaagcaca
    24181 gcttttcaaa acgggacaat tgaagaagaa ctggcctggc attatataat gcttgcttct
    24241 cgaaaggcgc acgagttgtt cgaaagcgaa gaagattatc aaaaaaatag tctaatggga
    24301 tttctttact ggcatatctg ctgctatcgc agaaagttaa cggatgctga acttgaagcg
    24361 tgttatcgtt acgacaagca gttttgggag cactacagta agaaatgccg ttggcccata
    24421 agaaatgttc cctggggagc atcatccgtt aaatactcat aaacgcctga aattttgccg
    24481 ggggcgctgc gcttgaccgg cctacattag agcgtaactg caatgaattg tgcccatctg
    24541 taggcctgat aagcgtagcg ccatcaggca tttacaactt aaatcacacc ctgcgccagc
    24601 atcgcatcgg caaccttcac aaaaccggca atgttcgcgc cctgcacgta gttggtttgc
    24661 tcaccttcac caccatgctc aacacaggca tggtggatat ccagcatgat gtgatgcaaa
    24721 cgtgcgtcaa ctttctcggc tttccagccc aggcgcgcag cgttttgtgc catttccagg
    24781 cccgacgttg ctacaccgcc agcattagcc gctttacccg gtgcaaacag tacgcctgcc
    24841 tgctggaaca gttcagtcgc ttcgatggtg gtcggcatat ttgccccttc agcgacggct
    24901 ttaacgccat tggcgataag ctgatgcgcg gcgtcaacat ccagttcgtt ctgggtggcg
    24961 caaggcaggg cgatatcaac cggtacagac cacggctgtt ggccttcgag atagaccaga
    25021 ccaaattctt tggcgtaatc tgccactcga ccatcgcggc tggctttgat ttcgataaga
    25081 cgtgccagtt tctctttcgt gaatccgctt tcatcaacta cagtgccgct ggagtctgac
    25141 gcagtgatca cacgagcacc aaattccatc gctttttcga tagcgtactg ggcgacgttg
    25201 ccggagccag aaacggaaac gctcatccct tcaaaaccca taccgtggcg ttttaacatt
    25261 gcttctgtga aataaaccag accgtagccg gtagcttccg ggcgaataag actaccgcca
    25321 aatgaaaggc ccttaccggt gaagacgcag gcggtattgt tggagagctt tttcatcatc
    25381 cccgccataa agccgacttc acgaccacca acgccgatat cacctgccgg aacgtcggta
    25441 tccgcgccca ggtggcgata cagttcagtc atcagcgtct ggcaaaaacg catcacttta
    25501 ccttcgcttt ttcctttcgg atcgaaatcg ctgccgcctt taccaccgcc catcggcagg
    25561 gtagtcaggg catttttgaa ggtttgttca aagccgagga atttgagaat ggaaaggtta
    25621 actgacggat ggaagcgcat accgcctttg tacgggccga tggcagagct gaactgcaca
    25681 cgccatgcac ggttgacctg tatctggttg cgatcatcaa cccataccac gcgaaactgg
    25741 atcacgcgct ccggttcaac cagacgctcc agtaatgaca tctggcgata ttttggattt
    25801 tgttcaagaa aaggccagag tgtggtcatt acttcacgaa cggcttgcgc gaactcggtt
    25861 tgattcgggt cgcgcttttg gacatggttg aggaatgact ccagagaata tgtctgatcc
    25921 atagatataa aaaccttata tattaatacg attgtgctta tgttgtttat gtcatgtgct
    25981 tttgcagttt tcgactatac catcaagaat gtgacggtaa gcaagaaaaa aatgacccag
    26041 gaaagcaaat taataacgag agtaatctca taaccaaatg cgataactca gacgctaaag
    26101 ttttgccacc cggctaccgt tatcagtagc agagacaata acgttaagga gttccctgtg
    26161 agtcgagcat tgttcgccgt agtattagca tttccattaa ttgcactggc taacccgcat
    26221 tatcgtccgg acgtggaggt aaacgtaccg ccagaagtgt tcagttcagg cgggcagtca
    26281 gcgcagccct gtactcagtg ctgcgtttat caggatcaaa attattctga aggggcggta
    26341 attaaagccg aaggaatact gctgcaatgc cagcgtgatg acaaaaccct tagcactaat
    26401 ccgctgctct ggcggcgcgt aaagccataa acgcctctaa taatggaatg tcagcagggg
    26461 ccagcggata ttgcagcgcc tcttcaggtg agcaccagac cagcgcctga tgttcatgtg
    26521 cctgtaacgt cccgtggaag tcgggtacgt gccaggcatg aagatggata atccggcccg
    26581 aaacttctcg ctgatggctg gcaacatact cacccacagt tgcttcgatg tccaattctt
    26641 cgtttaactc acgcaccagc acctgctgct ggctttcatc cagctcgact ttacctccgg
    26701 caaactccca taatcccgcc tgatcgctct gggcggggcg ttgcgcgagt aaaattttgc
    26761 catcacgttc aatgatggcg gcaacaactt caatcatttt cataaattac acacgggcag
    26821 ataagcgaaa aatgccataa tatccgcact gttgctcaat cagaaaggct ttatatttaa
    26881 ggagtgcctg tgctagaccg ccatcttcat ccccggatta aaccgttgtt gcatcagtgc
    26941 gtgcgggttc ttgataaacc gggcattacg ccagatggtt taacattagt gggatttgcc
    27001 atcggcgtgc tggctctgcc gtttctggcg ctgggctggt atctggcggc gctagtcgtc
    27061 attttgttga acaggctgct tgatggtctg gacggtgcgc tggcgcggcg cagagggctt
    27121 accgacgcgg gcggctttct tgatatttct ctcgattttc tcttttacgc gctggtgccg
    27181 tttggcttta ttcttgctgc accggagcaa aatgcgctgg caggcggctg gttgttgttt
    27241 gcgtttatcg gcacgggcag cagttttctc gcctttgctg cactggcagc aaaacatcag
    27301 attgataacc ccggttatgc acataagtcg ttttactatc tggggggatt aaccgaaggc
    27361 acagaaacga tcttactgtt tgtgctggga tgtttattcc ctgcatggtt tgcgtggttt
    27421 gcgtggatct tcggtgcgtt gtgctggatg acgaccttta cgcgggtgtg gagtgggtat
    27481 ctgacgctga agtcgctcca gcgtcagtaa ttgcaatgtt atttgctact gcccgggccg
    27541 cgttcaccgg ttgcaaccgg attttttgga tcgctgctcc attcgtacca gccgccgtca
    27601 tacacggaaa cattcttcca acccatggcg cgtgcgtaca taaaggtttc ggacgcgcgc
    27661 cagccggtgc cgcagtagaa tgaaacttgc tgatctggtt tgatattcca tgctttccac
    27721 atagcggtaa tatcatcggc gctacgcatg gtgccatccg ggttatagaa atcttccata
    27781 tgcgtcgagt cgctaccagc gtgtccccaa cgtgctccgg ctatttcacc ttttggttta
    27841 atatagctgt aaccgctggt cgtaccgata aattctggcc acgaacgaat gctcaccagc
    27901 gatgcatcct ggcgatgcag cagtccacgg gcctgttcca tatcgagcat caactgcggt
    27961 tgtgccggga tcttcacgcc gaaatccggt tccgctttca ctttcggtgg cgttcctcgc
    28021 tcaacaggca gtcccgcgtc ggaccaggtt tgccagccgc catccagcag acgcacatct
    28081 ttcacgcctg cgtagagcat aatttgcgcc acacgcgctc cagcgtatac gtcacgcccg
    28141 tacagaatga ccgtggtgtc atggcgaatg ccgtgttttg ccagcatcgc ttttagttgt
    28201 tcatcagaaa ctttgttcca cagcggttcg ctttccactt cgttggtatc gatgtagtca
    28261 gcgccaggaa tatggctgat aaggtaaagc ttaggagcgc cctaggccgc ttcaatgact
    28321 ttccagtcac cgacaggttt cgccgtaacc tctttacctt gttgcaggtc atgcagccat
    28381 tgcggataaa ccagctgctc aaagtgcggc agtttttgta gacgggaagg ttcgcttagc
    28441 gcgtcactca gaatggagat atgcgtaaaa cctgcttttt gcagtcgcgt tttgacggcg
    28501 tcgacatctt tgccattacc gtacagcgcc accggggcat cggctttcag gttatgttgc
    28561 ttgatccacg cgttgagctg ttcggtgctc attttgtcga gccagctagc agagaggttt
    28621 aaggcggcag gttcatgacc agaagggcca tttaaggttt gtggccagcc gttataaaac
    28681 gcgctggggc gagtatctat cgctttgcca ttttgttgct gaagttggtc aagtgtaaga
    28741 ggcttcgcca gttcagcggc ccacgaagaa gcgcaagcca gccctaaagc cattgccagc
    28801 gcggtcattt gagaaacacg tttcatcgaa aatcccgaca ttgtagtttt gagtgcgcca
    28861 ttctgaactg acgcattgag aaaaactttg cgttatcgca gtttgttgta attttctgac
    28921 cactgcgcca tatccagaac agaactatca gcaggaacat cctggagatc gtgcgttacc
    28981 tgaacgacgg ggatcgccag ggcgcgaact tcgctgaaca cccactggcg aaaattatcg
    29041 cgcagagcca catcaagacg gctgaatggc tcatccagga gtaacgcttt tggctgggcg
    29101 agaagggcgc gtagtagagc aacgcgcgct cgctgaccgc cagacaaagt ggcaggatcc
    29161 tgatggaaag ctccgtcaag gcctgaacgc tcaagtgcat cattcacggc attacgtcgg
    29221 gcattcccct taagtgtcgc cggtagcgcc agcagtaaat tttgcccgac gctgaactgg
    29281 tcaaataaca gtgcatcctg aaaaagaatg ccaatctgac gctgtgcggt gggtagcatg
    29341 tcaatccgtt gctcattgag ccatagctca cctgtacaag aaaactgtcc ggccagtgca
    29401 ccaatcatcc atgaaaacag agtggatttt ccacagccag acggccccat taacgtgacg
    29461 atgtcacctt tatcaaccgt aaagttaacg tttgtcagca agcggctttc tggtaaacgt
    29521 agcgaaacat ttttcacgca gagcattagc ggattccttg tctgacataa cctacccatt
    29581 ttgcgactaa cgcggtcagg gcaaaaataa taagcggtaa caacagttgc cataacgcct
    29641 gggcggcgag aataccgttg ctgccgccgc tgcttaatgc caccgcctca gtggtgagcg
    29701 tcggaaaacg ccccgcgccc agccacagcg tcggcatata ctgcgcaata ctgactgaaa
    29761 atcccaccgc gaaggcagtc agcacagggc gcaacatgag tgggcatttc acgtaaaaga
    29821 agattttggc ccgcgaccag cccagtgttt gcgcaatcaa aattaaccgt gaatcaatgc
    29881 gctgccaggc gggttgcagg ataaacagca tccacggcat tacccacagc agatgccccc
    29941 agaccaccgc cgtccagctt ccatccaggt tcagccatag cgccagcgtg tactggcccg
    30001 ccaccagtgg cagagcgggc aatagaatgg gcaaccatag ccacaactgg cgacgctgtg
    30061 ccccccattc cagccacagc agtaacagga gcaaagcgat gaatgttgct accagcccca
    30121 ttgtcagact gttgatgagt gcttcactat tgatcgtcga ctgatccgcg aggatcgcca
    30181 gcagaaccac acacagcaca ccggttaagg gtaaaaaact cgccagcgta gtgcctggta
    30241 ataaaggtgt ggcaggcttg cgaatgccat ctacgcgggg aatagtgcgc cgccagctgc
    30301 gccaaaacgt aggcggcgag taacagcatc agcagcagac tggcgagtgc gcctttcgtt
    30361 tgttgatccg cgtcgccctg agttaaccac tgccagctaa ttactgccag cgtcggtgga
    30421 ttacctggcc caagaataat cgccacatcc acgaccgaaa gcgaccaggc aacaatcgcc
    30481 agcatcgcca ttgccagcga aggcgcgacg gagggcaaca gcagccagtt caggcattgc
    30541 cagcggctgt agcccagcga atccagcaca atgacctgct gcaacagccg tttttcgctc
    30601 aatactgcca ctaagatcca cagcagaaat gcgctttctt tcactgcaag ggtgaggccc
    30661 agaccgatcc aaatcggtcc attggcggag tgaaatacgg gaaatagtca tatagcagcc
    30721 ctccgtcagc aaagagcagc agggcgctgg tggcaaaagc cacatgggga atggcgagca
    30781 gccacggcag acgggcgcac atacgctgcc atttcggccc aggccatagc gccacaatca
    30841 ccagcagggc aatcaacaat gccccgaccg ccgcgatggt tgtcgacacc agcgttgcca
    30901 gtaatgcctg cggtaactgt ggatcggcaa ataacgcctg ccagcgtgtc aacgacaacg
    30961 caggcgatat cagcgtgaga gctgccggga tcagcggtgc ataaatcacc gccaccatcg
    31021 cccacagcaa aaagattaat gcgtaccgta acggcgtaac cattcttgtt ccagtgcatt
    31081 cacccatcct gcgtgcggtt cagccagtac cggcggcaga tcctgcggca ttcttgattg
    31141 caatatttcg cgctgcccgt caggcagttt ttgcggatcg agaacagaag gatcgcccca
    31201 gacaacggga tctgctttac gcagttgcgc atcgggtgag agcaggaaat tggcaactac
    31261 cttcgccgca gcactggcat tcgcgttggc tggagtggtg acgaaatgca cgttgccaat
    31321 catcccctcg cgaaagccaa aactgtaact gcttgccggc aaatcgccgc tggcgatttt
    31381 ttgctgcgca tgtgcggggt taaaggtcag cgacaggcgc aatgtgccgg ctttcagcag
    31441 agcatccatc cgcgcgggcg aaggcgggaa atcctttcct tcgcgccaca aatacggatg
    31501 cagtgcatca agatattgct acaagggcgc agtgacacgg gcgaaagtcg catcgtccgg
    31561 cgcttctttt aatgcggcgg gatctggcgt cagcataatc agcaactgtt caagaaacgc
    31621 cgtgccggta aagtccggtg ggcgcggata agtaaccgtg ccgggattgg ctttagcaaa
    31681 ctccagtaag gcttgcggcg tttgtggtgg ctgtggcgta acatcgcggc gggcgataaa
    31741 cgtcagttgt gcgccgcccc agggcgattc cgcaccttgt gtcggcactg aaaaatctcc
    31801 ccgcaccggc agctgtgtgt cgacatagcg ccagttgggc agagtctccg cccagcccgt
    31861 ttgtaataaa ttggcctctt t
//
LOCUS       DAEEGA010000044        31599 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEEGA010000044 DAEEGA010000000
VERSION     DAEEGA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31599
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.044"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..606)
                     /gene="capU"
                     /locus_tag="KLH46_002169"
     CDS             complement(<1..606)
                     /gene="capU"
                     /locus_tag="KLH46_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBE0378840.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEF"
     gene            complement(609..1451)
                     /locus_tag="KLH46_002170"
     CDS             complement(609..1451)
                     /locus_tag="KLH46_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBE0378841.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1637..2354
                     /locus_tag="KLH46_002171"
                     /pseudo
     CDS             1637..2354
                     /locus_tag="KLH46_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3027..4535
                     /gene="ltrA"
                     /locus_tag="KLH46_002172"
     CDS             3027..4535
                     /gene="ltrA"
                     /locus_tag="KLH46_002172"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBE0378842.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPEPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            4616..5103
                     /locus_tag="KLH46_002173"
                     /pseudo
     CDS             4616..5103
                     /locus_tag="KLH46_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            5186..5443
                     /locus_tag="KLH46_002174"
     CDS             5186..5443
                     /locus_tag="KLH46_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378843.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(6001..6768)
                     /gene="fecE"
                     /locus_tag="KLH46_002175"
     CDS             complement(6001..6768)
                     /gene="fecE"
                     /locus_tag="KLH46_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HBE0378844.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(6769..7725)
                     /gene="fecD"
                     /locus_tag="KLH46_002176"
     CDS             complement(6769..7725)
                     /gene="fecD"
                     /locus_tag="KLH46_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HBE0378845.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(7722..8720)
                     /gene="fecC"
                     /locus_tag="KLH46_002177"
     CDS             complement(7722..8720)
                     /gene="fecC"
                     /locus_tag="KLH46_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HBE0378846.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(8717..9619)
                     /gene="fecB"
                     /locus_tag="KLH46_002178"
     CDS             complement(8717..9619)
                     /gene="fecB"
                     /locus_tag="KLH46_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HBE0378847.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(9664..11988)
                     /gene="fecA"
                     /locus_tag="KLH46_002179"
     CDS             complement(9664..11988)
                     /gene="fecA"
                     /locus_tag="KLH46_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HBE0378848.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(12075..13028)
                     /gene="fecR"
                     /locus_tag="KLH46_002180"
     CDS             complement(12075..13028)
                     /gene="fecR"
                     /locus_tag="KLH46_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HBE0378849.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(13025..13546)
                     /gene="fecI"
                     /locus_tag="KLH46_002181"
     CDS             complement(13025..13546)
                     /gene="fecI"
                     /locus_tag="KLH46_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HBE0378850.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            13844..14026
                     /locus_tag="KLH46_002182"
                     /pseudo
     CDS             13844..14026
                     /locus_tag="KLH46_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14020..14124
                     /locus_tag="KLH46_002183"
                     /pseudo
     CDS             14020..14124
                     /locus_tag="KLH46_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14464..14721
                     /locus_tag="KLH46_002184"
     CDS             14464..14721
                     /locus_tag="KLH46_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HBE0378851.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(15041..15172)
                     /locus_tag="KLH46_002185"
                     /pseudo
     CDS             complement(15041..15172)
                     /locus_tag="KLH46_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            15454..16812
                     /locus_tag="KLH46_002186"
     CDS             15454..16812
                     /locus_tag="KLH46_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HBE0378852.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(17051..18436)
                     /locus_tag="KLH46_002187"
     CDS             complement(17051..18436)
                     /locus_tag="KLH46_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBE0378853.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(18486..18833)
                     /gene="tnpB"
                     /locus_tag="KLH46_002188"
     CDS             complement(18486..18833)
                     /gene="tnpB"
                     /locus_tag="KLH46_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBE0378854.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(18830..19210)
                     /locus_tag="KLH46_002189"
     CDS             complement(18830..19210)
                     /locus_tag="KLH46_002189"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBE0378855.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(19565..19999)
                     /locus_tag="KLH46_002190"
     CDS             complement(19565..19999)
                     /locus_tag="KLH46_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBE0378856.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(19987..20388)
                     /locus_tag="KLH46_002191"
     CDS             complement(19987..20388)
                     /locus_tag="KLH46_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378857.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(20648..21217)
                     /locus_tag="KLH46_002192"
     CDS             complement(20648..21217)
                     /locus_tag="KLH46_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBE0378858.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(21962..22105)
                     /locus_tag="KLH46_002193"
                     /pseudo
     CDS             complement(21962..22105)
                     /locus_tag="KLH46_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(22439..22705)
                     /locus_tag="KLH46_002194"
     CDS             complement(22439..22705)
                     /locus_tag="KLH46_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378859.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            22846..23052
                     /locus_tag="KLH46_002195"
     CDS             22846..23052
                     /locus_tag="KLH46_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBE0378860.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            23147..23749
                     /locus_tag="KLH46_002196"
     CDS             23147..23749
                     /locus_tag="KLH46_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378861.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWGSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            23920..24117
                     /locus_tag="KLH46_002197"
     CDS             23920..24117
                     /locus_tag="KLH46_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378862.1"
                     /translation="MFFTARYTPGSQQTECAGAVIHQKWHAAVHTHPGDNMYVATAIP
                     ALWKRLTQPAGYSFRHPGMSA"
     gene            24949..25866
                     /locus_tag="KLH46_002198"
     CDS             24949..25866
                     /locus_tag="KLH46_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378863.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            25951..26823
                     /locus_tag="KLH46_002199"
     CDS             25951..26823
                     /locus_tag="KLH46_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBE0378864.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            27041..29425
                     /locus_tag="KLH46_002200"
     CDS             27041..29425
                     /locus_tag="KLH46_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HBE0378865.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNRTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            29422..30327
                     /locus_tag="KLH46_002201"
     CDS             29422..30327
                     /locus_tag="KLH46_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HBE0378866.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            30324..31394
                     /locus_tag="KLH46_002202"
     CDS             30324..31394
                     /locus_tag="KLH46_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBE0378867.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPLYVSLYP
                     TEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALSRSETVL
                     QKSLEITEEADLALRSAMARIK"
BASE COUNT         7739 a         8123 c         8349 g         7388 t
ORIGIN      
        1 aaattcgtgc cctttttccg ggcgcaacat tcctacctga gcaattacgg ggcatcctct
       61 tcgggacgcc agccaggaaa gaacactcgg aggcaaactg tttcgggaat caacgtataa
      121 ttcctggaaa tcaaagccag gaggcacaac agtaacccga gttcgacatc cttcctgctc
      181 cagatgtgtc ttcatatttg ttccgggaac aatcacctca tcgcaaaaat gatttattga
      241 aaaaaatttt gtttttcgtg tcaaatatgt cttttgcctg ataattctga atggatgttt
      301 ccaggtaaaa aaccgtacca aaccaacaat attactatca tgtccactgt gacaaacgat
      361 tgcatcgggc tgaaaactat gaactattcc gagtaatctc catacagtag gaatgtgaag
      421 actgtttcga aataacgcga atgtgatatc aattccaaac ttactggctt caaaagcaat
      481 tttgctgttc tccctgcaga caagcagaac tgaatgcccc attttcttca gggccttcat
      541 ttgagcaaca gcctgtaatt cctgaccacc tatattcggt gatgattccg taaatagtat
      601 attcattatt agtcatccgg taatcttggc cccttatgaa gggccagcaa cgatgaaaaa
      661 aacggtgttg tataataaaa aagtcgtctt ttcagccata cgcaattttc ccgttccttg
      721 gtgcttattc ttcctatccg tgtagttcct ttagcaggag cattcattct tctttctgtt
      781 gtatataaat aatggaatcc aagttcttca gcgatctgaa tataatcctt gttataataa
      841 ccttccggcc aacacaaatg tttactgcat tttcctgtca ttttttttaa atattctctc
      901 ccgctcaata agtcctgccg aagatgttta cactgttctt ctcgtgaagt gaacttttta
      961 tcccaacgtg tatgagtatg tgtatgaaca tgaaattcga ccagaccaga ttgcaacatt
     1021 tcgttcactt cagaccagcg aagcataaca ttatcagcat tcccggttgc tatttgatgt
     1081 tcgcaatcgc gatgagaata ttcctttcca ggagagtgac gaaccggacc atttccaata
     1141 aaactggtaa ttaaaaaaat atgtgccttg agattaaact cgtttaataa aggatatacc
     1201 tgaaaccagt tgtcaaggta tccatcatca aatgtcaaca taacgctttt acggggaagt
     1261 tttccgcccc gataaaaaaa ctccagttca tctgatgaca gtgttttcca gttgttttca
     1321 gccaaccatt tcatttgttt ccggaatgtc tcgggagaaa gtgtaaccag tcctgggcaa
     1381 tggctgacat gatgatacat taataccgga acatgatttg ctttaaacaa aatgccccct
     1441 ccgtttaaaa tttaaagttt ctggcaccca gtaagtagag ataaaaggaa agcctaataa
     1501 aggtggtgaa ctttatatac tactaaatca aacatcaaca atttataaac aataatatat
     1561 tatcatatct tttaaaaacg gctcactaag aaccttgagg tggcacacta aatttagcta
     1621 cctgaacaga ggtgatatgc tcacctcaga acaacacagg tgccataatg aaaaaagaaa
     1681 tttcagcgca gagtttaaac gcgaatccgc tcaactggtc gttgaccaga aatacaccgt
     1741 ggcagatgca gccagcgcta tggatgtcgg cctttccaca atgacgcgat gggtgaaaca
     1801 attacgtgat gagcggcagg gaaaaacacc aaaagcctcc cccattaccc cggaacaaat
     1861 tgaaatccgt gagctcagga aaaagctaca acgtattgaa atggaaaatg aaatattaaa
     1921 aaggctaccg cgctcttgat gtcagactcc ctgaacagtt ttcgataatc gggaaactca
     1981 gggcgcgtta tcctgtggcc actctctgcc atgtgttcgg ggtccatcgc agcagctaca
     2041 aatactggaa aaaccgtcct gaaaaaccag acggcagacg ggctgtatta cgcagccagg
     2101 tacttgaact gcatggcatc agccacggct ctgccggagc aagaagcatc gccacaatgg
     2161 caacccagag aggctaccag atggggcgct ggcttgctgg cagactcatg aaagagctgg
     2221 ggctggtcag ttgccagcag ccgactcacc ggtataagcg tggcggtcat gagcacgttg
     2281 ctatcccgaa tcatcttgag cgacagttcg ccgtaacgga accaaatcag gtgtggtgcg
     2341 gtgatgtgac ctatagtgtg cccggagttc agggcgggca tggatgctta aatgaaccgc
     2401 gagtctgtct ggaatattga accggtaact cacgatgaga aacccaacaa tcccaccggg
     2461 tgtgacggtg gagaacctga gcggcagtga cctgcggcat gcccgcaggg tgatgtaacc
     2521 cgctgacaac ggggattgag gcgagatcac taagccgaga tgatcctcaa ggttaagtac
     2581 tgaaaggctg aagaacatga acccgttaat ccgcctctgt gggttgaaaa cgtcaccacg
     2641 gcctacgtga tctgacaggc cgtgcaggag gaactggcag tgatacgtaa gcactgccgg
     2701 tcgaaggtgt tttgacatgt atgcgaaaca ccggggcagc agcgtctatc acgcttgcgt
     2761 tgctgacttc tgccaacttg cggcaagcaa ggataaagag tgcgacgggc agcctcctca
     2821 gtatgcctga gtccaggcag gtaaaccggg gaaggtcagc gacggatgtt aagggggcat
     2881 ggctccgatg acgcgctgac tggcggagct tccgtagtag tccgcgatgg ggaaagccca
     2941 ttacatggcg aagggaagca gtttgaatgt gtttgcgacg tgaattaact ggcctaacga
     3001 ggtgaagacc tttgataatc agcgaaatgc aacgcaagct tgccacatgg gcagccaccg
     3061 atccgtccct acggattcaa cggctgctgc gtctgataac acaaccagaa tggctggctg
     3121 aagcggcgcg gatcacgctt tcatcaaagg gggcgcatac ccccggcgtt gatggcgtga
     3181 acaaaacaat gctacaggcc agactggctg ttgagctgca aatcctcagg gatgaattac
     3241 tctcaggcca ctaccagccc ttgcccgcca gacgggttta catccctaaa agcaacggca
     3301 aactgcgacc actgggtatc cccgcgttgc gggatcgtat tgttcagcgg gccatgctga
     3361 tggcgatgga gccgatatgg gagagtgatt ttcatacgct ctcatatggc ttccggcctg
     3421 agcgcagtgt ccaccacgcg atccgcacgg tgaaattaca gctcacagac tgcggtgaaa
     3481 cccggggacg ctgggtgatt gaaggcgacc tgtccagtta cttcgacacc gtacatcatc
     3541 gactgctgat gaaggctgta cgccgcagga tcagtgacgc acgtttcatg actctgctgt
     3601 ggaaaaccat caaggcggga catatcgatg tcggtctctt tcgggcggcc agtgaaggtg
     3661 tgccacaggg cggtgttata tcgccgctat tatcgaacat catgctgaat gagttcgatc
     3721 aatacctgca tgagcgctac ctgagcggga aagccagaaa agatcggtgg tactggaata
     3781 acagtatcca acggggccga agtacggcgg tcagagaaaa ctggcagtgg aaacccgcgg
     3841 tggcgtactg ccgctatgcc gatgattttg tcctcatcgt caaaggcacc aaagcacagg
     3901 cggaagccat cagggaggag tgtcggggtg tgctcgaagg cagtctgaaa ctcaggctga
     3961 acatggataa gactaaaatc acccatgtta atgacggctt tatctttctg gggcacagga
     4021 tcattcgcaa acgcagtcgt tatggcgaga tgcgagtggt ctcaacgatc ccgcaggaga
     4081 aagccagaaa cttcgccgca tcgctgacag cactgttatc aggcaactac agtgaaagca
     4141 aagtcgatat ggctgaacaa ctcaaccgaa aactgaaagg ctgggccatg ttctatcagt
     4201 tcgttgattt taaggccaaa gtcttcagtt atatcgaccg tgtcgtgttc tggaagctgg
     4261 ctcactggct ggcccgcaaa taccgtacag gtatcgcttc cctgatgagg tggtggtgta
     4321 aatcaccgga accgggtcag agcaaaacgt gggttttatt tggtaaaacc aatcacggca
     4381 agctcagcgg cgaaatactg taccggttgg tggggcaagg caagaagctg ttccgctggc
     4441 ggctacccga aggtaatccc tatctgagga cggagaccag aaacacgtat acatcgcgct
     4501 ttacagaagt ggcaatggcg ttcgccagca tttaaatgga gagccggatg cgctgaaagg
     4561 tgcacgtccg gttcggggag gagaggcagg gaaatagtcc gactacgccc tgcctcttac
     4621 tctactctgg acgggtaagc gctgggcgta cctcgccgtt gttctcgacc tgttcgcaag
     4681 aaaaccagtg ggctgggcca tctcgttctc gccggacagc aggcttacca tgaaagcact
     4741 ggaaatggca tgggaaaccc gtggtaagcc cgtcggggtg atgttccaag cgatcaaggc
     4801 agtcattata cgagcaggca gttccggcag ttactgtggc gataccagat cagacagagt
     4861 atgagccggc gtggaaactg ctgggataac agcccaatgg agcgcttctt caggagtctg
     4921 aagaactaat gggtgccagc gacgggctat gtaagcttca gcgatgcagc tcacgcaata
     4981 acggactata tcgttggata ttacagcgca ctaagaccgc acgaatataa tggtgggtta
     5041 ccaccaaacg aatcagaaaa ccgatactgg aaaaactcta acgcggaggc cagttttagt
     5101 tgaccacaac agactacctg aagggagccg cggtcgccta gcagttgcag tagcaggaga
     5161 tcatccagcc gcagtacagg tcacgatgac tctggttaat gataccggct ttgaccccgt
     5221 attttccggc tctatcgctg aatcatggcg tcagcagccg tgcacaccat cctattgttg
     5281 tgactgggag gctgccacca tgcttcgcgc tttccctctg gcgaaaaagg gagaaggacg
     5341 ggcccgtctg ccttcacttt atgccagctt cggtaagctg ggtgagacac cgactcatga
     5401 agatatcatt gataacaatc gatccatcaa ctggcctgta taacgtggct gccggtgatt
     5461 aagaaagctg cacctaccta agtagtagca aacgcacact ttttagaaaa atcgatggtc
     5521 agaaactgga ttagcaattc cgttccaggg ttgcttttga tttacgttgg cgtctgatca
     5581 ttgatttatc ctcaaaagcc caacctcatt ggtaatgaac cagctccgtg aatgtccgct
     5641 ctggcacaga gcgaaatttt ttgatctccc cccctgaaat ctaaacttag tcatgtcacg
     5701 tttttgggtt tctaaaattt taacttcgcg tttttcgttg ccgtaagggt tatacagaaa
     5761 tgtccgttaa gcagagttca aaattgattg ccgtgatcac gactggtttg aaagccgcgc
     5821 ccaagcctgt acagctctgg tttgcgttga ttatgaacct gtcagcctaa agcaagcgga
     5881 tggacgatga gtattggtaa tctttcagag tccggaaaag ttcagcccca gtctgaacag
     5941 gcttgctggc gccagtccag tttcattcag tcgtggtttg gttcttacgg cctgtgcaat
     6001 ctacctcatt aggcacatcg gcctgccaga taccggctcg gggtgtattt ccgcttccac
     6061 gctgaatact gttctcagca atcctggggt catcacctct tctggtgtgc cttgcgccat
     6121 aacatgtccg tttgccatta ccaccagttg atcgcagtac cggctagcct gattaaggtc
     6181 gtgcagcaca gcgaccaccg ttttcccctg agtccggagt tcgcccatca accgcatcag
     6241 gtccacctgg tgattgatat caagataggt ggttggctca tcaagtaata caacgggcgt
     6301 attctgggcc aggaccatcg ccagaaatgc gcgctggcgc tgaccgccgg aaagctcggt
     6361 taaccgacga acggcaagat gattgatccg ggtctggttc atggcgacat taactcgtgc
     6421 attgtcttca gcggagagac gcccccagag tgacagccag ggattacgac catacgaaac
     6481 cagctcctgg actgtgatcc cctctggcgt taaatggtgc tgaggcagca gcgaaagcct
     6541 gcgggccaac tggcgtgatg agagcatatt tatgggatta tcgccgagaa atacggtgcc
     6601 agactgcggc attaaaagcc gcgaaaaaca gtttaacagc gtcgatttcc cgcaaccgtt
     6661 agggccgatc agggcggtga tcttccccgt tggcagtgag agtgaaacgt cgttaagtac
     6721 cttgtctgtc ccgtaactga ccgtcagatt ttcagttcgt aaagtcattt atcgcattct
     6781 cacaagcaac cagacaaacc acggcgcacc gataatggcg gtcagcacgc caaccgggag
     6841 ctccagtggg ggatgaataa ttctcgccag cagatcggca accaccaaca gcaacgcacc
     6901 tgtcagggct gaaacaggca gcagtctgcg gtgacgtcca ccggtgatgc tacgcatcat
     6961 atgcggcacc acgagaccaa taaagctaat cgggccgcag gcggccacgc cggtagatgt
     7021 catggcgaca gctagtaaca aagcccagaa tcgggtatgg ggcaccgaca caccgagcgt
     7081 ggtggcgcgc gcatcgccga gtgcaaggag gtcgagatcg cggcaaaaac tcaggctcag
     7141 cggcagaaat aaaatcatca gcgggatggc aatcttcaca aagctccagt cacggcccca
     7201 taagctgccg gtcagccaca gcagggcgtt gttcacatcc tgcgggcgcg agagcatcag
     7261 ataatccgtc aggctggccc agcatgcaga aagcgccacg ccggtgagcg ccagcttcat
     7321 cggctggtgg gtctttgcca gcatcttcag taatatcaac cccgccatgc cgcccgcaaa
     7381 ggccagcagc ggcagcacca tcacgggcag tgacggcata agaagtagag cccccacaga
     7441 ggccaggctg gcggcatggt taacgccgag aatatccggt gatgccagag ggttgcgcac
     7501 aatcccctgt atcagcacgc ccgccacggc gagggctgca ccgacaaaca gtgccagcag
     7561 caagcgcggc agtcggtact ccatcaatac ataataatgc tcgcgtccgg cctgccagtc
     7621 ggtcagcagc gcgcgccacg gcacggggat cactcccata tggagtgata acagcgcaca
     7681 gcccgccagg gcaagggtga tgaaaataac cagcgcaatt ttcatcctcg cctcctcaca
     7741 agccagacaa agcaagggct gccaatcagc gccagcactg cgcctgcggg cagatctccg
     7801 gggaaggcca gcgcgcgtgc gagtacatct gccagcagca tcagcgtggc ccccagcagc
     7861 atgctcactg gcagtacgtt gcgctgatcg aagcctgccc agaagcgcgc cagatgtggc
     7921 accagcagac cgataaacgc caccggacct gccacgctga cgcacgcacc aaccagaagc
     7981 agcactaaca tattgatgac caaacgtagc ctcgtcaggt tcactcccag cgtatgggcg
     8041 gtgctgtcgc tgaggttgag caggttcagt tgattcgcca gcagtaacac gacagggact
     8101 gcagtgacca ccaccggcaa gagctgccag acatcctgcc agcgggcgtg ggacactccg
     8161 cctgccagcc agtaaaagat gccgtaagca tgatcttcgg ccagcagcag ggtgatgcgg
     8221 gtaaggccca tacaaaaggc cgacagcgcg atacccgcga ggatcagttt gtttctgtca
     8281 tgggtatgac gaaatccgcc tcctgcggtc atgaccagca gccagctcac gccgcccccg
     8341 catgccgcga tgaacgacag agaatagcct gcaatcggcg tcggactcag cgcgctggta
     8401 agcgccatag ccagcgccgc gccgctgtta atgccgagca gtgaaggaga ggccattggg
     8461 ttgtgggtca gggtttgcag cagcgtgccc gcgagcgcca ggcttgcgcc gatcagaacg
     8521 gcgaccaggc ttcgtggcaa acgaaggttt tgcaccagcg cttctggtag cgttggcgtg
     8581 tgtccaggca gcagggcgcg ggttgcatct gctccggaaa caggaatggc cgagtagcaa
     8641 aacagactca gccagaaaat aataataagt gctgcaacgg gaagccccca cagcagcatc
     8701 gggtgtttta tcgcggtcat ttcacaacgg taagcggctg atggtggaag atttttaccg
     8761 tgtcagcggc aatacgctct gcagcaaaaa taccgcgcat ccgcgcccag gtgttactgt
     8821 cgaccgaagc aacctgctgc ttctgcgcgg cggttaacat ctgccagagc ggatcttgtt
     8881 gccagcgttt aacaatgctc tcttcgcgat agtgggcaac cagcagccag gcaggattga
     8941 ccgccagcag ttgctccagg ccgatggacg gcatggacga acccgccatc gcagcgggaa
     9001 cgttcagccc cagagaggcc agcacgctgc cggtccaggt ctcctgagta tgcaggttga
     9061 attgctgttc gcgtgatgtg ccaaaggcca cgcgtgtccc tttgggaagc tggctggccc
     9121 actgcgccat cctctcttta tgttgttcca gacgtgcctg catctctcgc tttttaccca
     9181 ccatttcgcc gatgatagcc gcagattgca aattttcagc gtaggtttcg ttgcgggact
     9241 taagcagcag taccggcgcg atttgctgca aggcgatgta aacccccgca tggcgactgc
     9301 tgtcggcaat gatcaggtct ggtttcagag cggcaatggc ttccaggctc ggctgcgcgc
     9361 gcgttccgac ggactgccac ggtttcaggt gcgcacgcac ttcgggcagg atgcgttttg
     9421 catcgttatc gtcggcaata ccgatcgggc tgacgtccac ggcggccagc gcatcggcga
     9481 acgagagttc cagcaccaca atccgttgtg gcgttttttc gagtgtaaac gtgccgtgtt
     9541 cgtcctgaac catggcggca aaggcgtggc tgatcaccag cagcaggcct gcaaaaagaa
     9601 aacggataaa tgccaacata atcacattcc agctaaaagc ccggcaagcc gggcgttaac
     9661 acatcagaac ttcaacgacc cctgcatata cagcgtgcgc ggctggcctg catagatgcc
     9721 tttgttgttg tcgtcataag agcggatgaa gtagtcctgg tcgaagatgt ttttcacacc
     9781 gaatgccagg ttcagatctg ccatctgcgg gccaaagtca tacgccacgc gtgcacccca
     9841 gagcatgaag ccgggaatgc ggccggtact gccgtcggcg ctctctttca ccgtattggc
     9901 gttatccgca aactggctgg actggaaatc gctgttcaga ttgaacgtcc agtttcctgg
     9961 cttgtagtcc acgcccagcg tgcctttatg tttcggggag aatggtacca gattgccgta
    10021 ggtgtcgcct ttctcgcgga tttccgcgtt cacatacgca tagctggcgt agatggaaac
    10081 gttatcaagc gttggcgtta gcgtacccag atcgtaacgt gcctgcgttt ccagcccggt
    10141 atggcgcgtt ttgccacgtg cagtgacggt gtcgttggtc tggttggagt cgtactgatt
    10201 gttaaagtta atcaggaaca gccccatttc cgccgtcagc gcgccgtcgt cgtagcgggt
    10261 accgagttcc caggttcgcg ctttttccgg ttccacattg ccgctttgca cagccttgcc
    10321 aatctggctg tactgtacgg tgccgaacga gccttcagtg tttgcataaa gattccagct
    10381 gtcagtcagg tgatagagca cgttcaacgc cggaagcggt gcgttatagc tcacttcttc
    10441 gtgcgtgcct gtgatggcgt tgttctggta tgactcgata tgttcgaaac gcatacccgg
    10501 cgtgatggtc cagttgccga tgtcgatttt gtcatccaga taccaggcgt gcgcctcggt
    10561 gccggaacgc gtatcgcggt cgtaagggct tgagccggac ggcaactgcc cgctgctggt
    10621 ggcggtgtag taacgcattt catgcgttga ttcattcaca tagcgatagc ccacgcccac
    10681 ttcgtgcgcg gaagggccga tcataaagat ctggctgtag cgtggctcaa taccgcgcac
    10741 ccagtagtta cgcggcgaga gggtgatgcg tttgccttgc tccaggtagc cgctgcgcag
    10801 ggtttgggtg tagaacccct gaatgttgaa tttatgctgg ctgtctggct ggaactggta
    10861 gcccaggctc gccagcttgc gacgacccca gaagcggtca tacgggcggg tggattgcca
    10921 gcgatcggcg tcgtaatccg cgcgagacag gccaccgggc atgtcggctt caccgtcgta
    10981 atattgcagc aggctgttga aggtgtgcac ctcatccggc gcatatttgc ttttcagcat
    11041 caggtcgtcg atgcgggtgg cgctgtgctc gcgccagtca ctgccgcgcg tgccggagta
    11101 gagcagcgcg gtgccaaaac cgttgtccgc tgtgccgccc accatcaggt tgtgcgtctc
    11161 tttcgggttg ttttgtgaag aggttgggct gagctgacct tccacgcccg cctcgatacc
    11221 aaagtcctgc ggaatggcac gggtaacaaa gttcaccacg ccgcccacgc tctgcggtcc
    11281 gtaacgcacc gcaccaccac cgcgtaccac gtcaatggca tccatgttgc cgagcgaaat
    11341 gggagccagt gaaagctgcg gctgaccgta aggggcgaag gggacgggga tgccgtccat
    11401 caggacggtc gagcggctgg cgaggcgcgg gttcaggccc cggatgccaa agttcatcgc
    11461 caggtcgtgg ctgccggtgc cgttgttttc cggcgcgctg acgccaggga tgcggttaag
    11521 tacctcacgc atggtggttg cgccggtttt ggcgaaatcc tcacggcgga tcacgtcacg
    11581 cgcgccagca tgttcaaata cgtcgttttc acgcgcatca cccagccagt cgccgaccac
    11641 ggtcagggca tcttcttttg gtgcgggcgc gggctccagc gtccagctgt tatttcccag
    11701 cggttttacc tgcagtccgc tgccgtccag cagttgttgc aggccgctct cgacgtcgta
    11761 atcgccgtgc aggccgttgc tctgcttgcc gcgcgtcagg ctggcgtcaa ccgagagggt
    11821 aaatccgctg tgtgcggcat actgattgag cgctttatcg agcgatcccg gtgcgatatt
    11881 aacctgtgca gcaaaagcgg aaaacgagag accggccagc ggcagcaggc tcaggcgaat
    11941 ggcgttaacc aaaggtgttg ttttacgaaa aacgcgtaac ggcgtcatac cttccccatc
    12001 atcatttttg ttgtgttcag ctatgagtcg aacgagaagg gtaaaaagga caatcgaaat
    12061 aagaattatt ttccttacag tggtgaaatg tttatccagt accgcgtaat agactgaatt
    12121 ttaacgggaa gcgtttgcgc gataacgttc aggatcgcat cggtattttt cagcgggaac
    12181 gtcccgctca ggcgcagccc ggcaacggcg ggatcgcagc gcagcacgcc gttgcggtaa
    12241 cgggttagcg tggctatcac ctcacccagc ggtttatcgc tgaagctcag gatgtccttc
    12301 gtccagcttg tactctcgtc atccagcggt ttcactgcgc caaactcaga ggcgctgaac
    12361 tgcaggcttt caccagcgtt cacgatgcgt ttttgcgcgg gggcactggc gagaagcact
    12421 tccacagcgt gctgctgaac gtcaagctgc gtgaaattat cctgctggcg gacggtaaat
    12481 tctgtcccta aagcagtgag ctggccctga cgggtcagga cacggaaggg gcgttgcagg
    12541 gcatctttcg cggtagtaat ggcgatttca ccgtaccaga gccggacggt gcgctgatgc
    12601 gcatcaaaac gcacatccgc cgcgctttgg gtattcagcg tgagcaggga gccatcttcc
    12661 agttgctgac ggctgactgt gcctttggcg gtgcggtaat ctgcccgcag accttcgccg
    12721 gtttccgact gccagagctg ccagcctccg ccagcgccga gcaacagcag taatcctttc
    12781 atcacgtgac ggcgggtgag gcgggtatcg tgcaacgccc ggctggcaac gtcgccaggc
    12841 acaccgccaa gctggttgcg caggttttca acctgctgcc aggcccactg gttatcctga
    12901 tcctgttcat accactgttg ccagcgcgct tcctgttgtg ggctgacgcg ctcgccgctt
    12961 agcacggcat accagtggga agctgaacgc agcgcctgac ggcgggaatc ggttaacaaa
    13021 ggattcataa cccatactcc agacggaaca gcaggcagtg ctcgacggct ttcgccacgt
    13081 attttttcac ggagctgatg gaaacaccga gtttgtgcgc aatctcgctg tatgtcagac
    13141 catccagttg cgaaagcaga aacgcttcac gtgttttgcc gtttagcccg tccagcatgc
    13201 tgtcgaggag ttgtagggtc tcgagttggc tttcgcgttc ctcaggtgaa ggcgctcccc
    13261 cctccggcat aagcgccagc atctccagat acgctttttc cagcgcgttt cggcgaaaca
    13321 ggtccaccat cacgcgtttg gcgatagtgc agaggaagga gcgaggatcg cggatcgtcg
    13381 agagcgtttc gctgaccatt acccgcaaaa aagtgtcctg ggcaatgtca tctgcatcaa
    13441 aagcagactg gagtttgcgc gtcagccagc ttttcaacca gccgtgatgt gtgccataaa
    13501 gcgactcgaa cgttaaggaa gctgtggtag tggcgcggtc agacatgcgg agtgcatcaa
    13561 aagttaatta tcacgtagtc atattaatat gagaatggtt atcattacaa ttggaaataa
    13621 aattgtttcc aatagacatt tttaacatgt tgtttttcta agtgttataa ggtaggtata
    13681 aaatgggatg gagcctctgc ttctgacatg tgtcggtcag aatgactcat gatgtggtct
    13741 gctattattg acatcctcac tgccctaaag gatggggatt tcggtaatga tcttacccag
    13801 caatagtgga cacgcggcta agtgagtaaa ctctcagtca gaggtgactc acaagacaaa
    13861 acagtttcaa ccagtaaaaa aacgtaaaca ccattcgcat gaatttcgta gttaagatct
    13921 gaagcttgca gaatgcatcg gtgttactgc cggagtccgt gaacttagtc tgcatgaatc
    13981 accacagctt tacatctgtc gcagtaaaca gcaaaatcag caaacttact taattatatc
    14041 gagtgcgatt acaatcgctg gcgatggtac agttggtgtg gcggtctcag tccggaacaa
    14101 tttgaaaatc ataatctcac ttaaaacggc atccatttta caaaagatgg ccgtctgtgt
    14161 atttgtaatg aataccttct acatgtttag agtgcataat ccacgacaag gcgatatcct
    14221 aaaaattagc tagcaggcaa ctttgatggc atagataatg tttttagtaa aaaagagttg
    14281 actgaatata atacagcctc ttcctacata agaaggaatg tgctcacaaa aaaaaaccat
    14341 tagtctgctt cctgttctta caataatcag tattatgtat tttatttata tagcaaaata
    14401 tgcttgttac acctgtcata attaggcatg gtaaaattat tgactgaacc ctgggggaga
    14461 aagatgcaca gtgattatta taatatggtt tttggtgaaa aattggccaa tattttatat
    14521 gaagctaact ctcaattttt ttacgagaga aatgtcatag aagaggcagt caacgcttta
    14581 ttctgtgagc gtgaaataat aaacaacaag aacataataa agaagctgat gttctttttg
    14641 tcagatgtta atcacaccaa gaaagatgtt gttcagagcg cacttaatat tatcattgac
    14701 attacatcag gtgatatttg agattttaca taaaagataa tctctcaagg agtcttatct
    14761 gaaaacgaaa tatatagtcc ctctcgtttt gtaatatcga ttggctttgt tgaatcggtt
    14821 acgtaccccg gagccggtca tattttcagg taatacacac gctttcagac atttcggctt
    14881 tcgtcatttt tgtatcatgg gcatagcttc tgctacctga ccatcataat cacgcagctt
    14941 cagtgaagca gtgaccacct gtttaacccg gtgtatcgtt atgatcttat ccagcaattg
    15001 aaccgccccg ggtttcctgg agagtgtttt atctgtgaac tcaggctgcc agatcatcgt
    15061 ttccgatgga agcataataa gctttttctg cttctgccgg tgggatatgg ccctgccttt
    15121 ccagcaatcg tcgattgtta taccagtcca cccacgtgag tgtggtcagc tcatcatact
    15181 gacacgtgca ttacccctac cgcgataatg caaccgtaca atatagggcg tttcctaaac
    15241 gcagccctca tcactccaat ccaacaagac acagcatttt tgatcacgaa gaaagacaaa
    15301 taatttccgt aaatgtttcc gttgttacaa ataacaacag tgttttacat ttctttggcc
    15361 tcaggcccgc gcatgttgcc attcgatgtc attttcctga ctcgtttctt ctttatggtg
    15421 tagtgtgatc ttcactttat agatgctaac aatatgaaaa gaaaaattat tcccgtgcta
    15481 atcggttgta cgctctcttt ctctgccctg gcggcacaac ctacggctga gcgctatgtc
    15541 gtcagttttc cagaaggcac gcacgtgaac tacgccggcg catttgccag tgcattcccg
    15601 aacggccttc cggttgggat aggttctggc ctgctgttca ccggtaaaca gggcgatgcc
    15661 ctgacattcg caaccattac cgatcgcggt ccgaacgcgg attcaccgaa agaggggaaa
    15721 aacgaaacta aaattttcgt tacgccagat ttcgcgccgc tgctgatgac gattcgtgta
    15781 caaaacggca aagcggaggc catcgatccc cgtcctctgc atgacgataa aggcgcgatc
    15841 aacggtctgc cgctggcaag cgacgtgatt ggctccacca atgaagtggc cttcagcgac
    15901 acgttacata gactgaaggg ggataatcgc gggctggata cggaagggat cactccggat
    15961 ggtaaaggag gttactggct gtgcgatgag tatggcccgt tcctgattaa catcgacagt
    16021 aaagggaaaa tcctggcaat ccacggtccg caggcggcgg aaggggaaaa agcgattgcg
    16081 ggcggtttgc caaatatcct caaatggcgt caggcgaacc gcggctttga aggcctcacc
    16141 cgtatgccgg acgggcgtat tattgtcgcc gtgcaaagta cgctggatat cgacgctaag
    16201 agcaagaaaa aggcgctgtt tacgcgtctg gtgagcttcg atccggcaag cggaaaaacc
    16261 gcgatgtacg gttatcctat cgacagcgcg gcctacagca aaaacagcga cgctaaaatc
    16321 ggagacatcg tggcgctcga taaccagcac atcctgctga tcgaacaggg aagagataaa
    16381 aacaacagaa tgcgtaacct gatttacgag gtcgatctca ataaggcgag cgacctgtca
    16441 ggcttcgaca agccgggtga gtatccggag tttgatgatg agaaaaccct gtcacagcgc
    16501 ggcattaccc ttgcgcagaa aacgcaggtt gttgatctgc gctccttggg ctggcagcag
    16561 gagaaggctg aagggttggc gctgatcgac agcaaaacgc tggcggtggc gaacgataac
    16621 gactttggcg tgaaggtggc gatgcaacat ccggtcgagg gcaagacatt caaggattat
    16681 cgggtgaacg cggaaggaaa gctgacgctt gacgacaagc aggttgaaac gaccctcaga
    16741 gtgaagccac tggagaaacc ggagtctgac agtgaactgt ggattgtgac gctgccggaa
    16801 gcgttgaaat aagtttataa ggtacggaca gcagtatcgc ttagtttttc aaattcatgt
    16861 atgtgaacaa tattggggtt ccggcgtaga cccccaatga aagcgtcacc acgtgaaaac
    16921 atgcctgcac ggtttacacg tcatcccatg gtggcagccg gttaactttc ctgcgccacc
    16981 agtggaaaaa ggccataagc agtccgaaca aaagtaagcg tacagcgagg gccgtattga
    17041 cggggatgtg ttattcagct ggcagtgcta tgcgccacgg aagcagttcg ctgacccggt
    17101 tgaccggcca gtctgctatg acgccaagca catggcgaag gtagctttct ggatccacgt
    17161 cattcagttt gcacgtcccg atcaggctgt acagtagcgc tccccgctca ccaccatggc
    17221 cagagccgaa gaacagggtc ttcatctttt cacgcaacca gctttccagg gatttcaaca
    17281 gcggtttcgt ttttcgctga cgttcagcaa gccgctgctc tgccggcatt ccccttatat
    17341 ccgcctctat ggcgtacaac tgaccgatct gctccagggc ttcttccgtc agtgctgacg
    17401 ggatgcggac gtgcacatcg tggatctttc ggcgggcatg agcccagcag gcagcttccg
    17461 ttatcccacc attgcgatac agctcgttga acccggcgta cgcatccgct tgcagcacac
    17521 cgctgaagca agcaagatga gtctgcggat ggatgctttt tctgtccggg ctgtaagcga
    17581 accacactgc aggtgccaac gctgacccgg cattgcggtc atcacgaaca tacgcccaca
    17641 accgcccggt cttcgtcttc ttattacccg gcagcagtac ctggaccggg gtatcatcgg
    17701 catggagttt gccgtcagtc atgacatagc catgaagcgc ctcttccagc ggagacagca
    17761 gccggcagca tgcatccacc cagcccgaca gcagtgaacg gctcagctcc acaccttgcc
    17821 ggccgtatat ttctgactgg caatacagcg gggtgtgctc tgcatacttc gaggtcagca
    17881 cgcgggccag cagccccggt ccggcgatac cccgctcgat gggccgcgaa ggtgcaggtg
    17941 cctgcacgat ggtatcgcac tgagtacagg catgtttttc ccgtaccgtc cggataaccc
    18001 ggaaggcgct acgcatcaac tccagctgtt cggcggtatc ctcgcccaga tagctcagtg
    18061 aaccgccgca gttcgggcag cacggcgccg caggcaacag tcgcttttcg tcacggggta
    18121 gtgattcagg gaacggctta cgggtgcggg tctgacgcaa cggacgctgt actgccgggt
    18181 catacaccct accagtcagc gtatcgctct ctttctgaag ccggttcaga tcggcttcca
    18241 tttgtgcgat acggcgggag actttttcgg aacgactgcc gaagttcatc cggcggagtt
    18301 tatccagctg cgcctgcaga tggtctattt cgcgctcccg gttgctcagc ttttcctgca
    18361 gggcgtggat cagcgcttcc tgttcggcca ggcgctgttt cagcaggaag atgtcgtcag
    18421 aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa ccaggataaa
    18481 gagatttaca gcatggtcag ggaggtcagc agccgcttgg gctgtcgcca gtcgatacct
    18541 tccagcagca tcgccagctg cgcctgcgta aggaacactt tgccatcacg ggctgacggc
    18601 caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc gccggtggac
    18661 cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaatatg gccggacatg
    18721 ggatcgtctt tcagcgccgt ttgtactttc gcagccaggc cgttgaagct atttctcata
    18781 tcggtgatac cggcaaccag ccaaattttg gtcccggaag gtaacgggat catcgcttca
    18841 gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag cggagcgtcc
    18901 cgtgccggaa cgttacctca cagctgatac tgagggtttc cgggtcctct gcgagcgatt
    18961 ctggctgttc ggcagctgca tcgagagtca caggaagtag ctgggggctc tctgaagaag
    19021 gtaatagcag ctttccctcg cgccattgtt gtcgccattt gaacaacaga ttggcgttaa
    19081 tgccattttc aagagcaagt tttgagatgg atatcccggg ttcacaggag gcagcaacga
    19141 gcagctgttt aaattcggga ggataattag ggcagccttt tcgcctgccg ggagtcacat
    19201 ttttctgcat atctgacact ttggttccca ctacttattt ggtggacacc actttgtcta
    19261 attcgtcaga ttctgaccag acggttcagg ctgtacgctt accactattt tatccctgtt
    19321 gaatttggtt aagccggtgc agacgcgccg ggaaacgcac acctgaaccg gcacaataaa
    19381 atacaataca accccctgtt actggagata cgaatggaat gacgaggcag cctgtcagtc
    19441 acagggggct gttggcataa aatcaacgga aaggccacca acaccggaat taagctccac
    19501 agtccccgca ctggcaatcc attcccggga aaggcatcac cgcaaactgc tccggaggca
    19561 accattacag cctctgtaac ggattatcag ccctggcatc ttccgacggc acctcctgcc
    19621 gcccccacgc cctgctgtat accccggtac cgcagcgtgg gcagcacttt tccccctcat
    19681 aatcttcgtc acacatcaca caaaaccact ttctcacctt caccgtataa tccggaaccg
    19741 tcatgccctg cagatgcaaa cgacgggcct ctttctctgc cgggtcaagc caggtacatc
    19801 gcagcaggat tttttcagcg acagtcatct ggtcatgttc aaaccacggt ggttgtccgt
    19861 cattgccccg ctgaagtgtt aacgggcatc cacaggacac acagcacaaa acgtcctgtc
    19921 ggttgtgctt cgcagcttct ttcgccgtaa caaaatggcc tttgctgtca ttcgccatat
    19981 aacagttcag aattcgcata tttcctcctg agttgtccgg ggaatactga atcctccggt
    20041 ctggcagtgt gtgcagtact gctccccata ataatcgtgg tgacattgtc tgcagtgcca
    20101 gctggcttta cgcaccacgg gtaaggcatc cggtacgaat tgctgcagac gtttaatcaa
    20161 ctgtatttct ctgcgctccg gcctgacata agggcactgt tgaccgtgct ctgtcagcct
    20221 gtcgtcagtg tgttcaaacc agggaagttc agtttcgtat tgcggatggt atctgagtgc
    20281 actgccacac aagtggcagg tgtagttagc ataaggtgcg gcctgtgcag tacgggcgcc
    20341 cgtcagacgt ccgttgccat caagagcgat aaaggatttt gcgtacatag tcatatacct
    20401 cagccgccag acgacacgca ggcaacagcc gtcccggtac gggcagcgtg gtcagggtgt
    20461 gaatggcggt tcagttaaaa tctggactgg agtttgggta aagcaggctg gttgctgtta
    20521 cagcactgtg gttgtcacac aatggaacac tgacgtaaaa aacagtcagt gggcgagtgg
    20581 atacatcgtt gccgccagac aacaacccat acttttggtt tgccggatat tggcgcgcca
    20641 gtcgtgctca actccggcta cagccaaaat tgcgctcacc ggtgcagtga gctttggtat
    20701 gctccttcgc cagatagtca gcacgttcca gcacttgctg aaagccggtg tcatcattac
    20761 gcgccagcca caccgccggc caggccggaa aatgcaccag cgtggcatgg cacccgacat
    20821 ccactcccag ggcactgcac cacgcctgtt taatcatccc ggccagagac tccggagcgc
    20881 ggtaatcacc ggcacgacac caggtatccc ggttgaccag cagcatcagg tgatagtgtt
    20941 ttttaccttt gcactcccca aactcccggg cccagacgta atgcagggtt gtgggatgca
    21001 cgcgtttacc ttcccgatgc ttacgtttct ggtaagcgtc gattcgggct ttcagggcat
    21061 tgatgaagcg ggatatcaca gctgcgtccg ttgctgccgg tacatccggg agacgcaggt
    21121 caacccgcaa tgccgtcagg cggggatgaa cattcagtgc gtgccgcacc gtctcacgaa
    21181 tacgttgctg ccagaagggg ttgtatttgt aggtcatggt taaatctccg tatggttcat
    21241 acggaatagc cacgtcgtaa aaaatacgca gagtccctga agtgacctcc ggcaggacac
    21301 tgtctcaggc actctgtgat aacccgaacg gtcgtctccg gactgacggt tgaattttca
    21361 gcgtcgtttt ttaatgtaaa aaaccggtta cgggtaaggc tgtgaggagg gagtgatggt
    21421 gatacgcaaa aagaagtgca gggactgtgg aaacgcgatt actcataata cagtgtgttg
    21481 cccgtattgt ggttctgtcg accccttcgg ctactaccgg aatacagacc gtatcgttac
    21541 catactgctg gcgctgatta ttgtggtcct gctgactact gtcagcgtca gtgtgtgtat
    21601 actgtgtagc tggtgagata acggttcatg tatcccctga gctgacagtg taccgtgtat
    21661 attaagcgta accgacaggt tctccttcat accatattaa attttccagg gattgccgct
    21721 gccttatttc gtcccataca ggaaaactca tctgggtttc tgacaaatac gcgaaactca
    21781 cagcccctca atatcaacca cacgttgtaa gccggtaata cctgaaaatc agcctattac
    21841 cgcccacgcc tccggcacca ttcccttttg atttcgaatt ctttgttgtc gctataccgc
    21901 ttaatgttcc cggtcctccg gtgcagataa caccggagtc atcgtagcgc tgcttcaata
    21961 ttcaggtgac gtatttccag acggaagccg gcgcgcgatc gtaaagtttg ttcattgtca
    22021 gttctgccag ccggttgtca acggcagagt taaaggcttc cagctccgca agggtaagtt
    22081 tataacgcgt gtgggaattc acttttcccg cgtctcctga cgtattacgg gctgccactt
    22141 acctggtgtc ccgcaataca gccttagtgt tccgttatcg gggatccgga cgcaccgcaa
    22201 caaccgggga agtatttctg gtttagagca gtgtcaagag ggatttctca gcccctcact
    22261 caccggctca gtctcagggc aggagcatta atacgctcag ttcatgtctt atacaggaat
    22321 gggcacaggg ggagctggcg tgtcactgct gattttacgc atcagcgttc atgtatctgc
    22381 actctgatac atgaacgatt cacagcgctc tgttaccgga gagatttact ctcttagctt
    22441 actctggcaa atcctttacg ttactctctg atgacttctt atatttataa taccaatcag
    22501 acagccttat cccatcggtg atatcagtct cctcctcgcc aggaacgggc tgactcactg
    22561 taactacctc cgaaaacaac tctgcaatta tcagaacgcc tgcttctctc cctgtcctca
    22621 cgaaaactat cccctcttta tcgcgcgtgc gtgcggaagc atcttttcgc aacaaccacc
    22681 cggtataccg gtacggctca gccaccgcaa cctccccgtt tatctcctgg cggtcacatt
    22741 cccgattatt ttttacgttt ctccccggtt gttatgccgg tgaaggtggc gcgctgtttt
    22801 catgaccaca ccggctggtt taacaacatc cggaggaaca ttctcatggc taccccagtt
    22861 tcactgatgg atgaccagat ggtcgacatg gcatttatca ctcaactgac cggcctgaca
    22921 gataagtggt tttacaggct catcagggat ggggcctttc cggctcccat caagctgggc
    22981 cgcagctccc gctggctgaa aagtgaagtg gaagcctggc tacaggcgcg tattgcacag
    23041 ccccgcccgt aatttctgac cgttatccgt tcacccgcag cagacgtaat ccccggcctg
    23101 ccggcggcat tctgctgcct gttatatccc cgtgaggaat atgaaaatga aacaacagta
    23161 tcagacccgc tacgaatggc tccacgaaag ctaccagaaa tggctgaccg gcttcacccg
    23221 gcacgccgta tcctggggcg tgtgtcatcc gaatatctac tacttccata atctgacgcc
    23281 cggttggggg tcattcaatg gtgaaaaacc ggagattgcc atcgtacccc agagcctgca
    23341 ccggctgatt tatggccctg acaaacgggc cacgccgccc ctggatgatg atctgatagt
    23401 gaatttatgc accagtgaac atctgctggt tcatcatccg atgctggaag gcattctcct
    23461 gtctgaatgt gaacgtctga ggcagcgttc tctggcaaat aaactgatca gcctcttccg
    23521 tcagtttggt ggcacggagt tgcgcctcaa actggtctgg ctttgctggc ttgatttaat
    23581 gaccggaaac agccttgaag actggaaaga gaacctgaaa cggaaatcag aaaaagagct
    23641 ggaggaatgg atcatcaacc gtcagaggca gagcgcagca ctgacggatc tgatggatca
    23701 gtacgtgctc ctggcatacc gcacaacggt tgacgatagc cgcaactgat gccggttgct
    23761 tccggaaggt gtgacaaaat acggaaatga tgatgctccc ggcccccatg ctcacctgca
    23821 gagcgtgttc gtcgtcatca tactgagcca tgacgggcgt cttaagggca tcctgtcatt
    23881 gctgagcagg caacaccacc agaggagcca cggacaggga tgttctttac agcccgatac
    23941 acgccaggtt ctcagcagac tgaatgtgca ggtgcggtta ttcaccagaa atggcatgct
    24001 gcggtacata cccatcctgg tgacaatatg tacgtggcga cagcgatccc ggcactgtgg
    24061 aagagactca ctcagcctgc aggatattct ttccggcatc cgggaatgtc cgcctgaatg
    24121 caaacaggat gatatttgaa tccgtaatac tgttctttca tcataaataa tttatgcaga
    24181 tacagttaac cgttacagta gccggtttgt tttaccggga aataatatcc gtcagactga
    24241 taccggaaca tatctgaaac acgttatgat gttttgttat cagaaatgtt gtatattctt
    24301 atccgtggcg acagtttaat gccctctggt atcacctgat aatgtatatc ctgcgatgat
    24361 tcgttatatt tacgggacac gcctcttttc attgtgctcc ttttcagact tcattttccg
    24421 taagtgttcc ccggcataaa ggaggtacct ttctggcgtt tataactctc tttatcggtg
    24481 acgccggtaa tacagggaat accacaccga caaacgaggt attaacagac gatatttcag
    24541 tgtccataat ttttctgtcc ggttaaataa cgtcagtctg ctgccctctg actgtaatca
    24601 tcaaagccgg aatgcatacc gggttacaac gtaaaaaaag tgatgctggc ccccggtgca
    24661 gctggcgggc gaaacatgtt tacctttcaa taccccctgt ctgcagtctc cactcttcca
    24721 tcctcttaag ctacgtcggc cacatgagtg cacttccatg ataacaggta ttcctgtcag
    24781 cataacgaag ccggttacct tcccttcctc ctgcatttcc tgtcagaatg actatacagc
    24841 gcgcagattg cctttttatt gaccatctta atataccgtt ataaacttct ttttatcata
    24901 ttattatcac cttattatgt ttaattttta agcagtcgga gaaagaggat ggttcagaaa
    24961 attttgtcgg ataaggttat gaatgaaaga acaaatgcgt attactctta ttatcttgga
    25021 gagcgtaata tctccgtact gccccttaat gtttatgatc ccccggaacg ttttatcgct
    25081 tacataaaaa aaaacagaga aaatctgaac atcacacttt cagattttga acttgaacaa
    25141 ataatttctg gtatgcgtct gaaggccctg gccttcctgg tccccctgga aaaaatatcc
    25201 tggatcgcgg gcagtgaacg cgcatgctta ttttcctggt atctgttgat gcagtttatc
    25261 cagaataaca gggcaaaaat aagcgcagac ttgcttcaaa agaataaact gtatcttaaa
    25321 gaagagtatc tggaagggaa tgccttcccg tcggactcat cgacacagtt cagacaaata
    25381 ctcagagttc tcgatattct ttctgataag aatttgcggg atgagtggat aatccagacc
    25441 aaagacagat ggatgcgggc atttaaatca aagagtcctt tcagttacct cctcccggag
    25501 aacgaacatg aatgcatatg gacatggaat tatttgaagg gaaaaaatat tgcactggaa
    25561 aaactggcca gttttcctgg ttctgctgat atttaccacg caattcacct ttcatttgac
    25621 atatgggtta cctgtccact cacctcacct gacgatataa aaaatttcag aaacagtttc
    25681 aataaggcca aggcgcagcg caagtacaaa aaaatgcagg aagataaagt caatgtacag
    25741 ttttttcttg acgcagaaac cagggcgcag ctcaaagaat tatcgagggt cagaagactc
    25801 agtaccggcg aaatgctgca tgacctgatt gttgaagagt ataaaaggta ccgacattcc
    25861 agataatttc agacatatat tatctccgtg agtcccccgc cacctttccg gtgtgcgggg
    25921 gttttgtctt ttttcaccgg gaatacatgt atgaatccgt ctgatgccat tgaggcaatt
    25981 gaaaaaccgc tctcctccct gccttactca ctttcccgtc acatcctgga acatctgcgc
    26041 aaactcacca gtcacgaacc cgtgattggc attatgggta aaagcggggc cggtaaatcc
    26101 tcactctgta atgcactgtt tcagggggag gtcaccccgg tcagtgatgt tcacgccggc
    26161 acccgggaag tgcagcgttt ccgtctgagt ggccatggtc acagcatggt tatcactgac
    26221 ctgcccgggg tgggcgagag ccgggacaga gatgcagagt atgaagccct gtaccgtgac
    26281 attctgcctg aacttgacct ggtactgtgg ctgattaaag ccgatgaccg tgccctgtct
    26341 gtggatgagt atttctggcg acacatcctg caccggggac atcagcaggt gctgtttgtg
    26401 gtgacgcagg ccgacaaaac ggagccctgc catgaatggg atatggccgg tattcagcct
    26461 tctcccgcac aggcacagaa tattcgcgaa aaaacggagg cggtattccg tctgttccgg
    26521 cccgtacatc cggttgttgc cgtatcggcc cgcaccggct gggaactgga tacgctggtc
    26581 agtgcgctaa tgacagcact tcccgaccat gccgccagtc ccctgatgac ccgcctgcag
    26641 gacgggctgc gcacggaatc tgtccggggt caggcccgtg aacagtttac cggtgcggtg
    26701 gaccggatat ttgacacggc ggagagcgtc tgtgttgcct ctgttgcccg taaggttctg
    26761 cgcgctgttc gtgacaccgt ggtctctgtt gcccgcgcgg tatggaactg gattttcttc
    26821 tgaacccggt ggtgatgcac cagactcttg ttacagattt ttaaccccat gaaaacacaa
    26881 tgcaaacaca gccatacccg ggttgtcccc ttgcgttgtg tttctcaagt taatagaatg
    26941 catcgatcgt tactatcata tcgatcgcta taaacgatcg tttgtggtaa atgacctcct
    27001 ccgtggttgt catgaaaatg cgtctcagga aggttaaata atgcacgaaa agaacatcgc
    27061 cctgctttgt gatgaagccg accgactttt gcaactgaac attaatctgc tccggcaaat
    27121 ggttgatgaa ccggatgtgt tatctgacag taagaacgaa aacagactgc tttttgataa
    27181 acagaaagca ctgaaaagaa ttgaggagct ggagggcgaa caaatcaaaa ccgcccgcag
    27241 ggagatggtg ctggctgttg tcggcacgat gaaagcaggt aaatcaacta ccatcaacgc
    27301 cattgtgggg caggaaattc tgcctaaccg taaccgcccc atgacctctg taccgacgct
    27361 catccgccac gttcccggaa aaactgagcc ggtcctccat ctggaacata ttcagcccgt
    27421 ccgcaattta ttaagcacac tgcaacaaaa actcgacacc ccggcaggac agcaagtcgc
    27481 acagaccctg cagcaaaccg gggatacctg cgaactgctg gatattctgg caaatgatgg
    27541 ctggctcaaa aatgaatacc acggggaaga cgaaatcttt accggactgg catcgttaaa
    27601 cgatctggtt cgccttgctg cggcaatggg gagtgagttt ccttttgatg aatacgcaga
    27661 agtgcagaaa ctgccggtga tcgacgtgga attcagccat ctggtgggga tggatgcatg
    27721 ccagggaaca ctcacactgc tggatacccc cggccctaat gaggccggac aaccgcagat
    27781 ggaagtgatg atgcgggatc aactgcagaa agcctctgcg gtgctggcgg tgatggatta
    27841 cacccagatg aactcaaaag cggatgaaga cgtccgtaaa gagcttaatg ccattgctga
    27901 cgtatcagcc ggccgcctgt ttgtgctggt caataaattt gatgagaaag accgcaatgg
    27961 cgatggggca gatgccgtac gccagaaagt tccggcaatg ctgaacagcg atgtgctgcc
    28021 cgcctcccgc gtttatccag gctcatcacg tcaggcgtac ctggctaacc gtgcgcttca
    28081 tgagttacgg aaaaacagaa cccttcctgt tgatgaagcc tgggtcgatg attttgtcag
    28141 ggaagccttc ggtcgcatga aaaaagaata cgtctgtaaa gacagtgaaa tggcaaccga
    28201 aggggcgaca gacctgtggg aaggctcact tatcgatcaa ctgataacgg aagtcataca
    28261 gagctcccat tccagagcgg cggcactggc ggttgattct gccgccgcaa aactgatgca
    28321 gaatgcggaa aatgtcagcg aatatctgtc attacgccat caggggctac agcaaagcat
    28381 tcagtcactg cagtcgcata tcaccagcct gcttgcggat atccgggaaa tcgcggactg
    28441 tcaggagcag gtgaccgctg atgtcagaat ggccatggag gagatcgatg ccagaacccg
    28501 ggaattactg acgggggtct gcacctcact ggaagaggag ctgaatgact atttcagaag
    28561 cggtaaacgc aaagaacagc aaatgctgga ggaagaaaat tcagcgcagc cccgggagag
    28621 aaacgcattc gctttctttc acgatatttt cggaaccgga aatcagcatg acaggatgcg
    28681 ggactttgat ccggacagcc ccgaaataaa attcagtgat cgcagggaag cccttgaact
    28741 gatgacgcaa atcgaatcga ccgtgaccag cctgcaccgt gaggctgaag cacagttccg
    28801 tcctgaactg gaaaaaatcg tcaggggtat tgaaacaggt tttcgtggta cggccctgta
    28861 cgccacagaa aatattgccg gacgcatcaa tgcccgtctg gaggatgaag gtttcactgt
    28921 aaaaatcagt tttccggcag tcagccagtt acagacccgg ctcgcagtaa aaacaaatct
    28981 gagcgcgctt atggaggaaa gaacggagac agtaacccgt cgctgtcgac aggatggtgt
    29041 ctggggtacg ctttgtcgat gggcaaacac cagtgactgg ggctggaaag agtacagtgt
    29101 ggatgtgagc cgcagcgtga tcaatatcaa caaggtcaga gaggaagtga tgtcactgac
    29161 ccgggcatat ttcggggagc tgcaggcatc cattgagcag gatattaacc agcccgtccg
    29221 ccaggaaatc gatgcctttt tctgtacatt cagggagaaa gttgaacagc tgcgtaacac
    29281 gctgattcag agctctgaag atcataaacg cgatcagcag gcgcaggaac ggctgaccgg
    29341 gcgtcttcag gcgttaaatg aaagggttcc tgagctcatt actgacagta aggcgttaag
    29401 ggaagaactg gagacaatgc tgtgacttca ccatttattc aacaaattgc tgataaccgg
    29461 gtatgtcagg tactcacctg tcttcctgaa aaatttgtgg ttgatttcgc aaacggtatt
    29521 gatgttgcac aggagcatat ccgtacggcc ggagagcgca cgttcttccg gcggttaaaa
    29581 gaggggctga gcggcgaagg tactgcacgg cagaatgcca tcaatgcttc gcttgcacag
    29641 ggcgttgagg cgtccctgcg ctggctgacg gagctgacaa cgtcacttgc caccacaaac
    29701 tacgcgatta cccgggtaaa cgacagggtc agttcactgg tcagtgatac tgccaggctg
    29761 gcgcattatt ccgcagatac gcgggagcag ttactcaccc tggccgatca ggttcaccag
    29821 aaactgaatc atcttgaaga aaaactccac cgtgttgacc tggttcagcg ggcgcagtta
    29881 catcttgagc agatattctc ctggtggagc gccgggcgat atgcgtcatt ttcccctgcc
    29941 ggacgttgtt atgtggcact tgaagagctt cgctggggcg cgtttggtga tgtgatacgt
    30001 cagggcgaaa caggccaggt taaccagcta ctggatattc tcagacataa agcattaacg
    30061 cagatggcac aggagagtgg cggtagtgca accgtgcgtc tgaacactct ggactggctt
    30121 ggtggtcagg gccgggagca ggcagataac gagtggcatg acgcgattaa ctggctgggg
    30181 gactggtgca gtgaagagca gcatccggtg atctggtcaa ccacgcaggc tgcagaacac
    30241 ttaccggttc gcatgccccg tctctgctct gcagaacgcc tctctgaaag tatggtcgat
    30301 gaaatatttc agaagggggc tgcatgagta tagaaataaa aacagggctg gtgctgtccg
    30361 gcggtggggc ggtgggcgct tatcaggcgg gagtggttaa ggcactggca gagtgtggta
    30421 cacagatcag catggtttca ggggccagca ttggcgcatt caatggtgcc attatcgcgg
    30481 cctctcccga tctgtcagaa gctgccgtac gcctggaggc gctctgggat catcttggga
    30541 ataatcaggt gctgtcggta aaccgatcgg tttacttttc attgctgaaa aaattgttcc
    30601 aggcaatgaa cctctgccag atccccggac gtgcaggagc actgcttacg acacttcttc
    30661 gccatatatc gataatcaac ggcttcgaca atccgatgac tcagccgttg ttgtcagatg
    30721 ggcccctgac agcgttgatg gatcattatc ttgatacaga tgctctggca ggcgggctac
    30781 cgctgtatgt gtcgctgtac cccacagaag ggggcatgca ggatattatt gactgcattc
    30841 gtgctgaact gggttccgga accacgaaaa acgccgtttt tcagcatatc cagagtctgc
    30901 cccgcggaca gcagaaagag gctctgcttg cgtcagccgc actgcccctg ttgttccgtc
    30961 cccgtgaggt tcaggggaca atgttcggtg atggtggtat gggaggatgg cgaaatatgc
    31021 agggaaatac ccctgtaacg cctctggtcg atgccggatg caatatggtg attgtgtcgc
    31081 atctgagtga cggttcttta tgggatcgcc aggcttttcc ggacaccaca atccttgaga
    31141 tccgtccccg gaaaaggctg aaatatgcag gtgatggtgg caacagtggc ggtctgctca
    31201 gtttcacgtc ggcacatacc gacgcctggc gtcagcaggg ctacgaggac acgatgctga
    31261 cgatggagca tatccggaaa ccgctggcag cacgtcaggc actgtcccgg tcagaaacgg
    31321 tgttgcagaa aagtctggag ataacggaag aggcagattt ggcactgaga agcgcgatgg
    31381 cccggattaa ataaagacgc cccaaagaaa cgccaccgcc tgtggtggtg tttttcctgt
    31441 ctggtgttgt tgaaaatatt ttcagatata tattacctgg gtgagattta agattaacgc
    31501 atttattaat gtcataagga gagttatcat gaaattcatc gtaaaagcac ttaggtgatg
    31561 ctgccaactt actgatttag tgtatgatgg tgtttttga
//
LOCUS       DAEEGA010000045        31187 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEEGA010000045 DAEEGA010000000
VERSION     DAEEGA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31187
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.045"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            389..2047
                     /locus_tag="KLH46_002203"
     CDS             389..2047
                     /locus_tag="KLH46_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBE0378868.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="KLH46_002204"
     CDS             complement(2044..3000)
                     /locus_tag="KLH46_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBE0378869.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="KLH46_002205"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="KLH46_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBE0378870.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="KLH46_002206"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="KLH46_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBE0378871.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="KLH46_002207"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="KLH46_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBE0378872.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="KLH46_002208"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="KLH46_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBE0378873.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="KLH46_002209"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="KLH46_002209"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBE0378874.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="KLH46_002210"
     tRNA            complement(9859..9946)
                     /locus_tag="KLH46_002210"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="KLH46_002211"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="KLH46_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBE0378875.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="KLH46_002212"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="KLH46_002212"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBE0378876.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="KLH46_002213"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="KLH46_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBE0378877.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="KLH46_002214"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="KLH46_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBE0378878.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="KLH46_002215"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="KLH46_002215"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBE0378879.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNA
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="KLH46_002216"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="KLH46_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBE0378880.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="KLH46_002217"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="KLH46_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBE0378881.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANGGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFVQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="KLH46_002218"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="KLH46_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBE0378882.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="KLH46_002219"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="KLH46_002219"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBE0378883.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLIEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="KLH46_002220"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="KLH46_002220"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBE0378884.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMIAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27413
                     /gene="dmsA"
                     /locus_tag="KLH46_002221"
                     /pseudo
     CDS             24969..27413
                     /gene="dmsA"
                     /locus_tag="KLH46_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27424..28041
                     /gene="dmsB"
                     /locus_tag="KLH46_002222"
     CDS             27424..28041
                     /gene="dmsB"
                     /locus_tag="KLH46_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBE0378885.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28043..28834
                     /locus_tag="KLH46_002223"
     CDS             28043..28834
                     /locus_tag="KLH46_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBE0378886.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28870..29496)
                     /locus_tag="KLH46_002224"
     CDS             complement(28870..29496)
                     /locus_tag="KLH46_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBE0378887.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29811..30959
                     /locus_tag="KLH46_002225"
     CDS             29811..30959
                     /locus_tag="KLH46_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0378888.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7686 a         7643 c         8379 g         7479 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcgggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagcgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 gcgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ttgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct agtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacggtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatgg cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgtg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagccgcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccgctgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgatcgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttacgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgatcg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctaccgat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacgg acaataccgg cgatgacaat tacgacggcc
    25261 tacactaggt tcgcgcctgc ctgcgtgggc gttccatgcg tcaccgtgtc tacaatccgg
    25321 accgcctgaa atatccgatg aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca
    25381 ttagctggga agaagcctac gacatcatcg cgaccaatat gcagcgcctg atcaaagagt
    25441 acggcaacga gtctatctat ctgaactatg gcaccggtac gctgggcggc accatgaccc
    25501 gctcctggcc gccgggaaat accctggtcg cgcgcctgat gaactgctgc ggcggttatc
    25561 ttaaccatta cggtgactac tcttccgcgc aaattgcgga aggtttgaac catacctacg
    25621 gcggctgggc agatggtaac agcccgtcgg atatcgaaaa cagtaaattg gtagtgctgt
    25681 ttggtaataa cccaggcgaa acgcgaatga gtggcggtgg ggtgacttac tatcttgaac
    25741 aggcacgcca gaaatctaat gctcgcatga tcatcatcga tccgcgctat accgacaccg
    25801 gtgccgggcg cgaagatgag tggatcccta ttcgtccagg aacagatgcc gcactggtta
    25861 acggtctggc gtacgtcatg atcactaaaa acctggtgga tcaggcattc ctcgataaat
    25921 attgcgttgg ctatgacgag aaaaccctgc ccgccagtgc gccgaaaaat ggtcattata
    25981 aagcttatat tctgggtgaa gggccagatg gcgtggctaa aacaccggaa tgggcctcgc
    26041 aaatcaccgg tgttccggca gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca
    26101 aaccggcgtt tatcagccag ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa
    26161 cccgtgctat ctcgatgctg gcgattctga ccggtaacgt tggtattaat ggagggaaca
    26221 gcggcgcgcg tgaaggttca tacagcttac cgtttgtccg tatgccgacc ttggaaaacc
    26281 cgatccagac cagcatttcg atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa
    26341 tgacggcgct gcgtgatggt gtgcgcggga aagataagct ggatgtgccg atcaaaatga
    26401 tctggaacta tgccggtaac tgcctgatta accagcattc tgaaatcaac cgtacccatg
    26461 aaatcctcca ggatgataag aagtgcgagc tgattgtggt tatcgactgc cacatgacct
    26521 catcggcgaa atatgctgac atcctgctgc ctgactgcac cgcttccgaa cagatggact
    26581 ttgcactgga tgcatcctgc gggaatatgt cttacgtgat tttcaacgat caggtgatta
    26641 aaccgcgctt tgaatgtaag accatctatg aaatgaccag cgaactggca aaacgtcttg
    26701 gcgttgagca acagtttact gaaggccgta cctaggaaga gtggatgcgg catctgtatg
    26761 cccagtcgcg ggaagcgatt cctgaactgc caacgtttga agagttccgc aagcagggga
    26821 tctttaaaaa gcgcgaccca caagggcatc acgttgctta taaagccttc cgtgaagatc
    26881 cgcaggcaaa cccactgact acgccatcgg gcaaaattga gatttattcg caggcgctgg
    26941 ctgacattgc cgctacctgg gaattgccag aaggcgatgt gatcgatcca ctgccgatct
    27001 acacgccggg ctttgaaagt tatcaggatc cgctgaacaa acagtatccg ctgcagctta
    27061 caggtttcca ctataaatct cgcgttcact caacttacag caacgttgat gtgctgaaag
    27121 cggcttgccg tcaggaaatg tggatcaacc cgcttgatgc ccaaaaacgc ggtatccaca
    27181 acggcgataa agtcaggatc tttaacgatc gtggtgaggt tcatattgag gcgaaagtga
    27241 cgccacgaat gatgccgggc gtggtcgcac tgggtgaagg cgcctggtat gacccggatg
    27301 caaaacgtgt cgataagggc ggttgtatca acgtactgac cactcaacgt ccgtctcctc
    27361 tcgctaaggg gaatccgtca catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa
    27421 ccgatgacaa cccagtatgg attttttatt gattccagcc gttgcaccgg ttgcaaaacc
    27481 tgcgagctgg cttgtaaaga ctacaaagat ttgacgccag aagtcagttt ccgccgcatt
    27541 tatgaatatg ctggcggcga ctggcaggaa gataacggtg tctggcacca gaacgtgttt
    27601 gcctactatc tgtcgatttc gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc
    27661 ccgagcggtg cgatgcataa acgcgaagat ggttttgttg tggtcgatga agatgtatgc
    27721 attggctgcc gttactgtca tatggcttgc ccgtatggcg caccgcaata taacgaaacg
    27781 aaaggccata tgaccaaatg cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg
    27841 atctgtgttg aatcttgccc gctgcgggcg ctggatttcg gccctatcga cgagctgcgt
    27901 aaaaaacatg gcgatctggc ggccgttgcg ccgttgccgc gagctcactt taccaaaccg
    27961 aatattgtga tcaaacccaa tgccaatagc cgcccgaccg gggataccac tggctatctg
    28021 gcaaacccga aggaggtgtg agatgggaag tggatggcat gaatggccgc tgatgatctt
    28081 cacggtcttc gggcaatgtg tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa
    28141 aggcgacctg cgagcagaag cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt
    28201 actgatgggc attggcttta tcgcctctat gctccatctt ggttcaccga tgcgcgcctt
    28261 taactcgctc aaccgggtag gggcttcagc actcagtaac gaaatcgcca gcggttcgat
    28321 cttttttgct gtaggcggca tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc
    28381 attgcgtacg ctatggctgg tggtgacgat ggttcttggc gtcgtctttg tctggatgat
    28441 ggtgcgtgtg tataacagca ttgataccgt tccgacctgg tacagcatct ggacgccgat
    28501 gggctgggcg atgcgtctgc tgccagccat ttctgtactg gcactggtgg tgagtggcgt
    28561 gatgtcagtg atgcagggca cagagctggc aactattcat agttctgtgc agcaggccgc
    28621 agcgctggtg ccggactatg gtgcgctgat gtcctggcgg atcgtgcttt tggccgttgc
    28681 cctgtgcttg tggattgcac cacagctaaa aggttatcag cctgcggttc cgctactatc
    28741 cgtctcattc attctgcttc tggcagggga attaatcggt cgcggcgtat tctatggttt
    28801 gcatatgacc gtggggatgg ccgtcgcaag ctaatcagta acaaccgggg tttcggcccc
    28861 ggttctcttt tatttctgct tcgttaacgt gtcataactg gtcatcaaat tacgataatc
    28921 aggaatatgg ttagagaaca acgtcgccag tccttcaata tcattacgcc agtcacggtg
    28981 cagctcgcaa gccacgccga accatgtcat caattgcgca ccagcttgcg acatgcgatc
    29041 ccacgccgaa tgccgggtaa tttcattaaa ggtccctgat gcatcagtca cgacaaagac
    29101 atcaaaccct tcttcaatgg ctgaaagcgc cgggaatgcc acgcaaactt cggttaccac
    29161 accggcaata attaactgtt ttttacctgt cgctttgact gcttttacaa aatcttcgtt
    29221 atcccaggcg ttaatattgc ccgggcgagc aatgtaaggt gcatcgggga attgtgcttt
    29281 cagttctaga actaatgggc cgttaggacc ggtttcaaaa ctggtggtaa gaatagttgg
    29341 taagttgaaa tacttagcca ggtcacccag cgccagcacg ttatttttaa acttatcggg
    29401 ttcgatatcc cgtacaaggg aaagtaaacc agcctggtga tcaacaagca aaacggcagc
    29461 atcattttta tcaagacgaa catacggttt ggtcatcctc ttctcctttc gagagtcagt
    29521 ggtacgtctg aaatcggtca gacgcgatgc attgctctga aagcatagac gggaaatatg
    29581 agtttgctgt gaccatgaaa tttttcgact gaacgaagcg tatgaaattt gtgttagatc
    29641 aataaaaaca atcagatgag attaatcatc tcttcagcat tagcgttatg aatgagtaat
    29701 ataaatcgat aataccgccg ctgcgatatg cgcgtatttt cacccattga caatgttttt
    29761 ggcggtggca tgatgcgcat gaaatttgaa cttcctcacg gttttaattc atgtccacgt
    29821 atacccggcc tgtcatgctt ttgctgtctg gcctgctttt gttgactctg gcgatcgcgg
    29881 tgttaaatac actcgtgccg ctttggctcg cccaggaaca catgtccaca tggcaggtag
    29941 gcgttgtcag ctcatcctat tttaccggca accttgtcgg tacattgctg acagggtatg
    30001 tcattaaacg cattggcttt aaccgcagct attatctgtc ctccttcatt tttgctgctg
    30061 gctgtgccgg ccttggcctg atgattggtt tctggagctg gttggcgtgg cgttttgtcg
    30121 cgggcgtcgg ctgtgccatg atttgggtgg ttgttgagag cgcgctgatg tgcagtggga
    30181 cgtcacgtaa ccgtgggcgt ttgcttgctg cgtatatgat ggtttattac gtgggaacgt
    30241 ttttaggcca gttactggtc agcaaagtct caaccgagct gatgagcgta ttgccgtggg
    30301 ttacaggttt gacattggca gggatcttac cgctgttgtt tacgcgtgtc ctgaatcagc
    30361 aggctgaaaa ccatgattcg acgtcaatta cggcaatgct aaaactccgt caggcgcggc
    30421 ttggcgtgaa tggctgcatt atctcaggaa tcgttctggg atctctatat ggcctgatgc
    30481 ctctgtacct caatcacaaa ggggtgagca atgccagtat tggtttctgg atggcggtac
    30541 tggtcagtgc gggtatcctc ggacaatggc cgattggacg tctggcggat aagtttggtc
    30601 gactgctggt tttgcgtgtt caggtctttg tcgtcattct cggcagtatt gcgatgctta
    30661 gccaggcggc gatggcccca gcgttattca tcctcggtgc cgctggcttt acgctatatc
    30721 cggtggcgat ggcctgggct tgtgagaaag ttgaacatca tcaactggtg gcgatgaacc
    30781 aagccttact gttgagctat accgtgggaa gtctgcttgg cccgtcattt agcgctatgc
    30841 taatgcagaa tttctccgat aatttattgt ttatcatgat cgccagcgta tcgtttatct
    30901 atttgctgat gctgcttcgc aacgccggtc atacgccgaa acccgttgct cacgtgtaaa
    30961 tgaattcaag caaagtgtga acttactgtt tcacactctg cttttttgtt tcttctatct
    31021 gacttgcttt tttccaaatt ttattcgttt aaaaataaaa tgtgcagcag gttataattt
    31081 tgcatttcgc tatttccgca cttcttattt gccgcgcata atccctcgtt ttacagatgc
    31141 ccctttaatt ttggcgaagg atttgtctat ggctgggaat gttcagg
//
LOCUS       DAEEGA010000046        30584 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEEGA010000046 DAEEGA010000000
VERSION     DAEEGA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30584
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.046"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..588
                     /gene="tuf"
                     /locus_tag="KLH46_002226"
     CDS             <1..588
                     /gene="tuf"
                     /locus_tag="KLH46_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBE0378889.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            818..1201
                     /gene="secE"
                     /locus_tag="KLH46_002227"
     CDS             818..1201
                     /gene="secE"
                     /locus_tag="KLH46_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBE0378890.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPIRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1203..1748
                     /gene="nusG"
                     /locus_tag="KLH46_002228"
     CDS             1203..1748
                     /gene="nusG"
                     /locus_tag="KLH46_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBE0378891.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1907..2335
                     /gene="rplK"
                     /locus_tag="KLH46_002229"
     CDS             1907..2335
                     /gene="rplK"
                     /locus_tag="KLH46_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBE0378892.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2339..3043
                     /gene="rplA"
                     /locus_tag="KLH46_002230"
     CDS             2339..3043
                     /gene="rplA"
                     /locus_tag="KLH46_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBE0378893.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3456..3953
                     /gene="rplJ"
                     /locus_tag="KLH46_002231"
     CDS             3456..3953
                     /gene="rplJ"
                     /locus_tag="KLH46_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBE0378894.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4020..4385
                     /gene="rplL"
                     /locus_tag="KLH46_002232"
     CDS             4020..4385
                     /gene="rplL"
                     /locus_tag="KLH46_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBE0378895.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4705..8733
                     /gene="rpoB"
                     /locus_tag="KLH46_002233"
     CDS             4705..8733
                     /gene="rpoB"
                     /locus_tag="KLH46_002233"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBE0378896.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8810..13033
                     /gene="rpoC"
                     /locus_tag="KLH46_002234"
     CDS             8810..13033
                     /gene="rpoC"
                     /locus_tag="KLH46_002234"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBE0378897.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="KLH46_002235"
     CDS             complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="KLH46_002235"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBE0378898.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="KLH46_002236"
     CDS             complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="KLH46_002236"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBE0378899.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="KLH46_002237"
     CDS             complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="KLH46_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBE0378900.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="KLH46_002238"
     CDS             complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="KLH46_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBE0378901.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="KLH46_002239"
     CDS             complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="KLH46_002239"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBE0378902.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="KLH46_002240"
     CDS             complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="KLH46_002240"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBE0378903.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18720..18820)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="KLH46_002241"
     CDS             complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="KLH46_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBE0378904.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19444..20217
                     /gene="nudC"
                     /locus_tag="KLH46_002242"
     CDS             19444..20217
                     /gene="nudC"
                     /locus_tag="KLH46_002242"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBE0378905.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20257..21321
                     /gene="hemE"
                     /locus_tag="KLH46_002243"
     CDS             20257..21321
                     /gene="hemE"
                     /locus_tag="KLH46_002243"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBE0378906.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21478..22149
                     /gene="nfi"
                     /locus_tag="KLH46_002244"
     CDS             21478..22149
                     /gene="nfi"
                     /locus_tag="KLH46_002244"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBE0378907.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22192..22782
                     /locus_tag="KLH46_002245"
     CDS             22192..22782
                     /locus_tag="KLH46_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBE0378908.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22969..23241
                     /gene="hupA"
                     /locus_tag="KLH46_002246"
     CDS             22969..23241
                     /gene="hupA"
                     /locus_tag="KLH46_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBE0378909.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23254..23949
                     /locus_tag="KLH46_002247"
     CDS             23254..23949
                     /locus_tag="KLH46_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBE0378910.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="KLH46_002248"
     CDS             complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="KLH46_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBE0378911.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24614..25990
                     /gene="zraS"
                     /locus_tag="KLH46_002249"
     CDS             24614..25990
                     /gene="zraS"
                     /locus_tag="KLH46_002249"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBE0378912.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25987..27312
                     /gene="zraR"
                     /locus_tag="KLH46_002250"
     CDS             25987..27312
                     /gene="zraR"
                     /locus_tag="KLH46_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBE0378913.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27309..28598)
                     /gene="purD"
                     /locus_tag="KLH46_002251"
     CDS             complement(27309..28598)
                     /gene="purD"
                     /locus_tag="KLH46_002251"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBE0378914.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28610..30199)
                     /gene="purH"
                     /locus_tag="KLH46_002252"
     CDS             complement(28610..30199)
                     /gene="purH"
                     /locus_tag="KLH46_002252"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBE0378915.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7165 a         7962 c         8419 g         7038 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggcat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggg cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      601 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
      661 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
      721 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
      781 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
      841 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggc
      901 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
      961 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1021 cattgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgattcgcca
     1081 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1141 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1201 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1261 gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1321 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1381 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1441 ggcacttggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1501 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1561 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1621 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     1681 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     1741 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     1801 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     1861 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     1921 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     1981 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2041 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2101 ttctttcact ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa aagcggcagg
     2161 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2221 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2281 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2341 ggctaaactg accaagcgca tgcgtgttat ccgtgagaaa gttgatgcaa ccaaacagta
     2401 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2461 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2521 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2581 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     2641 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     2701 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgt ggcctgatgc caaacccgaa
     2761 agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     2821 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     2881 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     2941 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3001 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3061 tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat
     3121 attcgggttg tggcaaggcg gcaactgagt gagtcgccaa gagcatagct aactatgtga
     3181 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3241 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3301 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3361 tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag
     3421 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3481 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3541 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3601 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     3661 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     3721 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     3781 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     3841 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     3901 gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     3961 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4021 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4081 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4141 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4201 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4261 tgaaagaagc taaagacttg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4321 aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta
     4381 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta
     4441 gtcaccagcc tttttgcgct gtaaggcgcc agtagtgttt cacactgttt gactactgct
     4501 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4561 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4621 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     4681 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     4741 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     4801 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     4861 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     4921 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     4981 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5041 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5101 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5161 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5221 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5281 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5341 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5401 cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct
     5461 tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5521 cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc
     5581 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     5641 gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt
     5701 atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt
     5761 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     5821 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     5881 gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     5941 gaaatcgaag gttccggtat tctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6001 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6061 atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6121 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg
     6181 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6241 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6301 ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6361 actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6421 aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6481 aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc
     6541 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6601 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac
     6661 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     6721 cacgatgacg ccaaccgtgc attgatgggt gcgaatatgc aacgtcaggc cgttccgact
     6781 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     6841 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     6901 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     6961 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7021 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7081 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7141 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7201 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg gtccggaaga gatcaccgct
     7261 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7321 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7381 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7441 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7501 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7561 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc
     7621 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     7681 ccgcgcgatc gctggctgga gctgggcctg acagacgaag agaaacaaaa tcagctggaa
     7741 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     7801 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta
     7861 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     7921 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     7981 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8041 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8101 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8161 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8221 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8281 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8341 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8401 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8461 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8521 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcact
     8581 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacatcgt ggacggcaac
     8641 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     8701 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     8761 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     8821 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     8881 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     8941 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9001 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9061 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9121 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9181 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9241 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9301 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9361 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9421 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9481 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9541 actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga
     9601 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
     9661 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
     9721 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
     9781 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
     9841 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
     9901 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
     9961 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10021 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10081 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10141 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10201 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10261 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10321 ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg
    10381 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10441 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt
    10501 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10561 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10621 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    10681 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggca tcgatgacat
    10741 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    10801 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    10861 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    10921 aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    10981 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11041 tggtctgatg gcaaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11101 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11161 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11221 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11281 ggttatcgag gggggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11341 tgctgaagac gttctgaagc cgggtactgc tgatattctc gttccgcgca acacgctgct
    11401 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11461 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11521 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11581 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    11641 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    11701 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    11761 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    11821 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    11881 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    11941 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12001 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12061 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12121 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacacct tggcgcgtat
    12181 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12241 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12301 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12361 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12421 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12481 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12541 gggcgtaaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12601 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    12661 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    12721 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    12781 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    12841 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    12901 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    12961 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13021 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13081 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13141 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13201 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13261 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13321 cataccggct gcaaaccctg agcggttaac acggcagcaa ccgcttccgg tctgcgatcg
    13381 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13441 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13501 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13561 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13621 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    13681 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    13741 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    13801 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    13861 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    13921 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    13981 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14041 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14101 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14161 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14221 cgctgcgcct gttgggccag ttgttccaga tagccactgg cggcaggcga taacagcgcc
    14281 atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt
    14341 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg attgctgaag
    14401 gttttcatgc cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaataac
    14461 tgcggctgcc cggtccggac tgacgtgcca gcaggcctac ttctaccgcc agacgaaatg
    14521 ccttcgccat gttgacggga tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt
    14581 cggcccccat ttccagcgcc tgcgtggcat ggctgggaac gccgatgcca gcatcgacaa
    14641 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    14701 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    14761 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    14821 gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    14881 gaatctccaa ttttagccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    14941 tggcttcttc cgctgttttc gccccggaag tattcggcag cagggccaca cccgccgcga
    15001 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15061 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15121 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15181 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15241 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15301 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15361 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15421 caggctgcgc cactggctcg acttaccgtc gaacagtcgg agttctcccg caggcgtctc
    15481 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccatca ccccgaccac
    15541 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tgctggttat ctggccacag
    15601 gcagcggtaa cacccctgct cccagggcgg tgtcagtacc atcaactgac cgccaaatcc
    15661 gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg
    15721 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcttgtgcaa ccgcatcttt
    15781 caacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg
    15841 tgtcagtcgc tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag
    15901 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    15961 gacgccagcc cctgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16021 cagcacctgg ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcagaat
    16081 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16141 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16201 tgccgacacc cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca
    16261 cggtgggata atccgccagg cgctcaacat gccgtgccaa ctgttccagc ccctgcggtg
    16321 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16381 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16441 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16501 catacgcctg atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16561 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat
    16621 ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    16681 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    16741 acatcacgct tcctccttat gcaggtagat ttctccgcct ctggcacgga agttctccga
    16801 catatccgcc attcccactt caatggtttg cgcggcggcg taatcgcgta cttcctggct
    16861 gattttcatc gagcagaatt ttggcccaca catggagcaa aaatgggcga ctttgccgga
    16921 ctcttggggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    16981 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17041 cgcgcccgga tgcccttttg ccaggtcagc agcgtgggcg gcaatcttat aagtgataag
    17101 cccctgctta acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca
    17161 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17221 atagcccggt gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17281 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacat ggcctgggcc
    17341 ttcaatcatc acctgtacgt catattccca ggcgattttg gtcagttcgc ccagcgtatg
    17401 caattcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17461 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17521 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17581 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    17641 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    17701 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    17761 tgtaccgatc ggcaccggac tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    17821 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    17881 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    17941 caggaagtta cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18001 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18061 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18121 gcggcccata ttctcgcgga tggcgatgaa ttccatgtcc ggcgtgatga tgccctggcg
    18181 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18241 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18301 gcgcacggta agttcttcgg tatcggcgcg cgcatcgatc cacggctggc gtagttttgc
    18361 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18421 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18481 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18541 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18601 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag
    18661 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    18721 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    18781 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    18841 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18901 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    18961 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19021 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19081 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19141 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19201 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19261 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19321 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19381 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19441 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19501 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19561 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt aatacaacag
    19621 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    19681 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19741 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg tagccattgc
    19801 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19861 tcgctcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19921 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19981 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20041 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20101 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20161 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20221 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20281 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20341 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20401 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20461 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20521 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20581 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20641 cgtaccattc gtcacgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20701 tggacgctgg caacctacat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa
    20761 aaaatgatgt atgccgatcc gcaggcgctg cacgctctgc tcgataaact ggcgaaaagc
    20821 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    20881 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20941 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21001 accaaaggcg gcggacagtg gctggaagcc atggctgaaa ccggttgcga tgcgctgggc
    21061 ctcgactgga caacggacat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21121 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21181 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21241 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21301 tctgaacagt atcaccgcta agagcctgat ccatcaggct attttatttg ccattttgag
    21361 gccgggcagt gctcaaaatc ctcacgtact acgtgtacgc tccggttttt ccgcgctgtc
    21421 cggcctcaaa ctgccttcaa caattacgcc tgttgaacca ggctctaagg agcgattatg
    21481 gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat ctgcgaggat
    21541 cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc
    21601 ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag
    21661 tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc
    21721 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    21781 gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggctta
    21841 ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    21901 ctctccagca aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg
    21961 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    22021 gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    22081 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    22141 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa
    22201 aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct
    22261 tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt
    22321 gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac
    22381 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    22441 gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt aagtgaactg
    22501 gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    22561 atcacgaccg ttgcgatgct ggaaatgact caggctggtc gcgaaatggg cgatgaagag
    22621 cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    22681 ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    22741 gagtcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    22801 aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    22861 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    22921 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    22981 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    23041 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    23101 gttggtttcg gtaccttcaa agtgaaccat cgcgctgagc gtactggccg caacccgcag
    23161 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    23221 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    23281 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct gtttctcact
    23341 gcctgtagcc acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    23401 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt
    23461 agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg cgataacctc
    23521 acgctcatca atatcaatgt ttacagcaaa ccgccggtga acattcgcgc gcgttttgac
    23581 gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct
    23641 aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc
    23701 ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg
    23761 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    23821 gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa
    23881 ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    23941 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc
    24001 ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca
    24061 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    24121 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca
    24181 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    24241 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    24301 caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    24361 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc
    24421 cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    24481 actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    24541 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    24601 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    24661 cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg
    24721 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24781 tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    24841 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    24901 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    24961 ttcccctcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    25021 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    25081 tggaatgtac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat
    25141 cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    25201 tattctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg
    25261 ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt
    25321 ggcgctgggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    25381 taaaggactg gcgaaatact ttgccgagcg cgcgcctgca gggggagaag cgcatcaact
    25441 ggcgcaggtg atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg agttgctgga
    25501 actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca
    25561 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    25621 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    25681 tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag
    25741 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga
    25801 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg
    25861 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    25921 ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    25981 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    26041 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    26101 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    26161 atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt
    26221 ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact
    26281 ggggcgcagg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    26341 aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag
    26401 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    26461 gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    26521 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    26581 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt
    26641 actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt
    26701 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag
    26761 gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt
    26821 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26881 tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    26941 atccctctgc tggctggcca ttttcttcag cgctttgccg agcgtaatcg caaggcggta
    27001 aaaggcttta cgccacaggc gatggatttg ctgatccact acgactggcc gggaaatatt
    27061 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    27121 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggggca aagtcaggat
    27181 attcagccgc tggtggaagt ggaaaaagag gtgattctgg cggcgctgga gaaaacgggc
    27241 ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa
    27301 ctgtcgcgtt agttctgctc gcgttcgatg gcgcgccaac cgatatcttt ccggcagaag
    27361 caatcatccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgccacg
    27421 gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggttaccac ctgctcgtca
    27481 tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc cagcggcagg
    27541 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    27601 ctgagagaag cacgttcatc ccactctgac gttttctcgt ccagtttgcc ttcacaggcc
    27661 gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27721 tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg
    27781 cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc
    27841 cagatgatac gttccatggt gcgctgatga acttcatcgg ttactaccgg cgcgggggag
    27901 taagcgccca tcccgccggt gtttggtccg ttatctttat cgcctacgcg tttgtgatcc
    27961 tggctggtag ccatcggcag cacatgctcg ccgtccacca tcacgataaa gctcgcttct
    28021 tcgccatcga ggaactcttc gataacgatg cgatgacccg cgtcgccaaa agcgttgccc
    28081 gccagcatat cgtgaacagc cgcttccgct tcttccagcg tcatcgccac gataacgcct
    28141 ttcccggcag ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac
    28201 accagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat cttatggcgg
    28261 gccaggaaat ctttggtaaa cgcttttgag ccttccagtt gggccgcacc tgcggttggg
    28321 ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct
    28381 tccgggccga cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg
    28441 atatcggtca cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt gcctgcatta
    28501 cccggagcaa caaaaacagt ctcaaccagc ggcgactggg ccgctttcca ggccagcgcg
    28561 tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat taatggcgga
    28621 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt
    28681 cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg
    28741 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt
    28801 ccaggccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28861 ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagca ttggatttca
    28921 cgaatttcgc cactttccag cagaacagcg catcacgcag ttcctgttcg gtcggctgac
    28981 gttgggtgac gacgcgcagt tcttccgcac cgaccatccc caggtcacga tcctgaacca
    29041 gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac acgctcgccc cactgaccgc
    29101 aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg
    29161 aaggcgcaat aatcacttcg acaaactgac gagaaatgat ggcctgtgcg gtttccgcat
    29221 ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg
    29281 cgcgatcgta agcatcaaga atggaattgc cgatagctac accgcaaggg ttggcgtgct
    29341 tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    29401 cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggta gcaacggagg
    29461 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac
    29521 gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt
    29581 ctttgctttc accgtggtac gccggaacca tgctgccgaa gtagttggca atcatgctgt
    29641 cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca
    29701 gcgatccttc gttgtcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca
    29761 cgattgcgac atctttatgg ttcttagcgg cggagcgcac catggtcggg ccgccgatat
    29821 cgatgttctc aaccgcatct tccagcgagc agccttcacg agcaacagtc tgggcgaacg
    29881 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29941 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    30001 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt
    30061 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    30121 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    30181 ctggacgacg ttgttgcatg gtaaatcccc tgggtttgac tattacagag agcgttagct
    30241 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    30301 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    30361 ttttgacagt ggttaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc
    30421 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    30481 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgccttca tcgacacggc
    30541 ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gaga
//
LOCUS       DAEEGA010000047        29802 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEEGA010000047 DAEEGA010000000
VERSION     DAEEGA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29802
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.047"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            58..852
                     /locus_tag="KLH46_002253"
     CDS             58..852
                     /locus_tag="KLH46_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBE0378916.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            874..2283
                     /locus_tag="KLH46_002254"
     CDS             874..2283
                     /locus_tag="KLH46_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0378917.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2261..3040
                     /locus_tag="KLH46_002255"
     CDS             2261..3040
                     /locus_tag="KLH46_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBE0378918.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3037..3897
                     /locus_tag="KLH46_002256"
     CDS             3037..3897
                     /locus_tag="KLH46_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBE0378919.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4045..4620)
                     /locus_tag="KLH46_002257"
                     /pseudo
     CDS             complement(4045..4620)
                     /locus_tag="KLH46_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4637..4897)
                     /locus_tag="KLH46_002258"
     CDS             complement(4637..4897)
                     /locus_tag="KLH46_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBE0378920.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4888..6159)
                     /locus_tag="KLH46_002259"
     CDS             complement(4888..6159)
                     /locus_tag="KLH46_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE0378921.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6237..6599)
                     /gene="queD"
                     /locus_tag="KLH46_002260"
     CDS             complement(6237..6599)
                     /gene="queD"
                     /locus_tag="KLH46_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBE0378922.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6918..8717
                     /gene="cysJ"
                     /locus_tag="KLH46_002261"
     CDS             6918..8717
                     /gene="cysJ"
                     /locus_tag="KLH46_002261"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBE0378923.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8717..10429
                     /gene="cysI"
                     /locus_tag="KLH46_002262"
     CDS             8717..10429
                     /gene="cysI"
                     /locus_tag="KLH46_002262"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBE0378924.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10503..11237
                     /gene="cysH"
                     /locus_tag="KLH46_002263"
     CDS             10503..11237
                     /gene="cysH"
                     /locus_tag="KLH46_002263"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBE0378925.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11501..11653
                     /locus_tag="KLH46_002264"
     CDS             11501..11653
                     /locus_tag="KLH46_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBE0378926.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11847..12938
                     /locus_tag="KLH46_002265"
                     /pseudo
     CDS             11847..12938
                     /locus_tag="KLH46_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13084..14121)
                     /gene="iap"
                     /locus_tag="KLH46_002266"
     CDS             complement(13084..14121)
                     /gene="iap"
                     /locus_tag="KLH46_002266"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBE0378927.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14373..15281
                     /gene="cysD"
                     /locus_tag="KLH46_002267"
     CDS             14373..15281
                     /gene="cysD"
                     /locus_tag="KLH46_002267"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBE0378928.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15283..16710
                     /gene="cysN"
                     /locus_tag="KLH46_002268"
     CDS             15283..16710
                     /gene="cysN"
                     /locus_tag="KLH46_002268"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBE0378929.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16710..17315
                     /gene="cysC"
                     /locus_tag="KLH46_002269"
     CDS             16710..17315
                     /gene="cysC"
                     /locus_tag="KLH46_002269"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBE0378930.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17365..17688
                     /locus_tag="KLH46_002270"
     CDS             17365..17688
                     /locus_tag="KLH46_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBE0378931.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGSLF
                     MWLLG"
     gene            17882..18193
                     /gene="ftsB"
                     /locus_tag="KLH46_002271"
     CDS             17882..18193
                     /gene="ftsB"
                     /locus_tag="KLH46_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBE0378932.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18212..18922
                     /gene="ispD"
                     /locus_tag="KLH46_002272"
     CDS             18212..18922
                     /gene="ispD"
                     /locus_tag="KLH46_002272"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBE0378933.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18922..19401
                     /gene="ispF"
                     /locus_tag="KLH46_002273"
     CDS             18922..19401
                     /gene="ispF"
                     /locus_tag="KLH46_002273"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBE0378934.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19398..20447
                     /gene="truD"
                     /locus_tag="KLH46_002274"
     CDS             19398..20447
                     /gene="truD"
                     /locus_tag="KLH46_002274"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBE0378935.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20428..21189
                     /gene="surE"
                     /locus_tag="KLH46_002275"
     CDS             20428..21189
                     /gene="surE"
                     /locus_tag="KLH46_002275"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBE0378936.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPAEQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21183..21809
                     /gene="pcm"
                     /locus_tag="KLH46_002276"
     CDS             21183..21809
                     /gene="pcm"
                     /locus_tag="KLH46_002276"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBE0378937.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21949..23088
                     /gene="nlpD"
                     /locus_tag="KLH46_002277"
     CDS             21949..23088
                     /gene="nlpD"
                     /locus_tag="KLH46_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBE0378938.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTMSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23151..24143
                     /gene="rpoS"
                     /locus_tag="KLH46_002278"
     CDS             23151..24143
                     /gene="rpoS"
                     /locus_tag="KLH46_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBE0378939.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24263..24670)
                     /locus_tag="KLH46_002279"
     CDS             complement(24263..24670)
                     /locus_tag="KLH46_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBE0378940.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24817..25410
                     /locus_tag="KLH46_002280"
     CDS             24817..25410
                     /locus_tag="KLH46_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBE0378941.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25410..26357
                     /locus_tag="KLH46_002281"
                     /pseudo
     CDS             25410..26357
                     /locus_tag="KLH46_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26630..27193)
                     /locus_tag="KLH46_002282"
     CDS             complement(26630..27193)
                     /locus_tag="KLH46_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378942.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27358..27609
                     /locus_tag="KLH46_002283"
     CDS             27358..27609
                     /locus_tag="KLH46_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0378943.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27611..29707
                     /locus_tag="KLH46_002284"
     CDS             27611..29707
                     /locus_tag="KLH46_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0378944.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7301 a         7382 c         8059 g         7060 t
ORIGIN      
        1 attcgtcaca tcatcatcgg caacgaaggt gcattgtgct atatcactga agtaacagtg
       61 aaaatcttta aatacacccc ggaaaacaac ctcttctacg gctatatcct ggaagacatg
      121 aaaaccggct tcaacatcct gcgtgaaatc atggtggaag ggtatcgtcc gtcgatcgct
      181 cgtttgtatg acgctgaaga tggcacccaa cacttcaccc attttgccga cggaaaatgt
      241 gtgctgatct ttatggctga aggtaaccct cgcattgcga aggcgacggg cgaagggatt
      301 gcggaaatcg ttgcccgcta cccgcagtgc cagcgcgtgg acagcaaact gattgaaacc
      361 tggtttaaca acctgaactg gggaccggat aaagtggctg ccgaacgtgt gcagatcctc
      421 aaaaccggca acatgggctt taccaccgaa gtgtccggct gctggagctg catccacgaa
      481 atctacgaaa gcgttattaa ccgtattcgt actgagttcc cgcacgccga cgacatcacc
      541 atgctgggcg gtcactcctc tcatagctat cagaacggca ccaacatgta cttcgtctat
      601 gactacaacg ttgttgactg taagccggaa gaggaaatcg acaagtacca caatccgctc
      661 aacaaaatca tctgcgaaga aaccattcgc ctcggcggtt cgatggtgca ccaccatggc
      721 attggtaaac atcgcgttca ctggagcaag ctggaacacg gcagcgcgtg ggcgttgctg
      781 gaagggctga aaaagcagtt cgatcctaac ggcattatga atacgggtac tatctatccg
      841 attgaaaaat aatgtatcag gcagcgttcc gcgatgacgg ggcgttgcct tttcggcttc
      901 tcaggcgaga agccgttctt attaccggac aatgaagggg taaagatgaa cacttcaccg
      961 gtgcgaatgg atgatttacc gcttaaccgt tttcactgtc gaattgctac gctcactttc
     1021 ggcgcacacc tgaccgacgg ttatgttcta ggcgtcattg gttacgccat tattcaactt
     1081 acgcccgcca tgcaactgac gccgtttatg gcgggaatga tcggcggctc ggcgctcctt
     1141 ggtttgtttc ttggcagcct ggttcttggg tggatctccg accatattgg tcggcaaaaa
     1201 atcttcacct tcagcttttt gctgattacg ctcgcttcgt tcttgcaatt ttttgccacc
     1261 acgccagagc atcttattgg gctgcgcatt ttgatcggca ttggtctggg aggcgattac
     1321 tcagtaggtc acaccttgct ggctgaattt tccccgcgcc gccatcgcgg tattttgctg
     1381 ggcgcattca gcgtggtgtg gaccgtaggc tatgtgttgg caagtattgc cggacatcac
     1441 tttatttccg aaaacccgga ggcctggcgc tggttgctgg catcggcagc tctgcccgcg
     1501 ttgttgatta cgttattacg ctggggaacg ccagaatcgc cacgttggct actgtgccag
     1561 gggcgttttg cagaagctca cgctatcgtg catcgctatt ttggtcccca tgttttactg
     1621 ggcgatgaag tggtaacggc gacccataaa cacatcaaaa ccttgttctc ttcgcgttac
     1681 tggcggcgca cggcgtttaa cagcgtcttc tttgtctgcc tcgtaatccc atggtttgtg
     1741 atttatacct ggctgccaac tatcgcccag actattggtc tggaagatgc gctgactgcc
     1801 agcctgatgc ttaatgcgtt gttaattgtg ggcgcgctgc tgggattagt tctgacgcac
     1861 ctgctggcac atcgcaaatt tttgctggga agttttttgc tgctggcggc aacgctggta
     1921 gtaatggcct gtttgccttc cggcagttca ttaacgctgc tgctttttgt tctcttcagc
     1981 accaccattt cggcagtcag taatctggtg ggcattttgc ctgcggaaag ttttcctact
     2041 gacattcgct cgctgggcgt cggttttgcc accgccatga gtcgacttgg cgcggcggta
     2101 agtactggcc tgctgccgtg ggtgctggcg cagtggggaa tgcaagtcac cttattgctc
     2161 ctagcgacag tgttgttggt tggttttgtt gtgacctggc tatgggcacc agaaactaaa
     2221 gccctcccgc tggtggcggc gggaaatgta ggaggtgcga atgaacattc tgttagcgtt
     2281 taaagccgaa ccggatgccg gaatgctggc ggaaaaagag tggcaggcgg cggctcaggg
     2341 taatagcgga ccggatgttt cgctactgcg aagtttactc ggtgctgatg aacaggccgc
     2401 tgccgcgctg ctgttagcgc agagaaaaaa cggtacgccg atgtctttaa ccgcgttgag
     2461 catgggggat gaacgggcgt tgcactggct gcgctatctc atggctcttg ggtttgagga
     2521 agctgtattg ctggagacgg cggcagatct gcgctttgcg ccggaatttg tcgctcgcca
     2581 tattgccgaa tggcagcgtc agaatccgct ggatctgata attaccggct gccaaagcag
     2641 cgaagggcag aatgggcaaa cgccattttt gctggcggaa atgctgggct ggccctgctt
     2701 cacccaggtg gagcgtttca ctctcgacgc gctgtttatc accctcgaac agcgtactga
     2761 acatgggttg cgctgttgcc gggttcgctt gcctgcggtg attgccgtgc gtcagtgcgg
     2821 tgaagtggcc ttgccagtac cgggaatgcg ccagcgaatg gctgcgggga aagcagaaat
     2881 tatccgtaaa actgttgctg ccgaaatgcc agcgatgcag tgtctgcaac tggcaagagc
     2941 agagcaacga cgcggagcga cgctgattga tgggcaaacg gtagcagaaa aagctcaaaa
     3001 gctatggcag gactatttac gccaaaggat gcagccatga atatcgcaat tgtgacaata
     3061 aatcaggaga atgccgcaat agcgagctgg ctggcggcgc aggatttcag cggttgtacg
     3121 ctggcgcact ggcaaataga acctcagccc gtagtggcgg aacaggtgct ggatgcgatg
     3181 gtggaacagt ggcagcgaac gcctgcggac gttgttctct ttccgccggg aacgtttggc
     3241 gatgaactgt cgacccggct ggcctggcgt ttacatggtg caagcatctg tcaggtgact
     3301 tcgttagata ttccaaccgt aagcatgcgc aaatcccact ggggaaatgc gttaacggcg
     3361 acgctgcaaa cagaaaaacg cccgttatgc ctttccctgg cgcgtcaggc gggagcagcg
     3421 aaaaacgcca cgttgccctc tggaatgcag caactgaaca ttgtcccagg tgcgcttccc
     3481 gactggctgg ttagcacaga ggatctgaaa aatgttagcc gcgacccatt agctgaggcc
     3541 agacgggtgc tggtggtggg acagggcgga gaggcagata accaggaaat cgttatgctg
     3601 gcagaaaagc tgggagccga agtgggctac agtcgggcgc gagtgatgaa cggtggcgtt
     3661 gatgctgaaa aagtgattgg tatttccgga catctgctgg cacctgaggt gtgcattgtt
     3721 gttggcgctt ccggcgcagc agcgttaatg gcgggcgtac gcaatagcaa gtttatggtg
     3781 gcgattaatc atgacgccag cgcagcggta ttttcacagg cagatattgg cgtggttgat
     3841 gactggaaag tggtgctgga ggcactggtg acaaacattc acgcagaata ccaataacgt
     3901 gctgccggat gatcctgcct acggttctgg actggggacc tgtaggcctg ataagacgcg
     3961 ttaagcgtcg catcaggcgc tgaatgtcgg atgtggcgtg cggccaacaa atcaaaacaa
     4021 attcaattgg ttacgattat cccgtcaaag caattttttc gctaacgtcc agactccggt
     4081 attcgtggtg gaaagcgcca cgacacccgc gttaatcgca ttacgcgcat cttcttcatc
     4141 gcataccagc ccgccagcaa tcagcggttg gcggattttt tccgtcaccc agcccagcac
     4201 tttgggcatg cagcctggca ggatctcaat acaatccgga ttcgattgcg caacttactt
     4261 atcaatgttg tgaaacgaaa tcgaatcaac aataaacagg tgatgaatac agaaaaaaac
     4321 ttccgctctt gccgctttta gcatcgatgc tttggtgctg ataattccat ccgcctcggt
     4381 caccagtttc aaaaactgaa tcaccacttc tttatttgac gcgccttcca gcaaatcaac
     4441 atgaataaaa gcatatttcc cggcgttctt aatcttttta acgatattac tgatggtgca
     4501 gatattgccg tacaacacgg aaataaactg gcattcagaa tcgatggcca gttgcaggct
     4561 ggcattatct ttaacggcag caatcaccgg attctggcgg agcaggtgta agaggggcat
     4621 aaattatcct tattatttat ccaaagcggt aggtgatgcc gaatccggaa ggcggatagc
     4681 gccactgttg tagtgtggat tcgtcgcaca gcaaacggca ggtgccacac tccaggcagc
     4741 cgcgatagtc aatacgtaag tcaccttcct gtgtgagaga aaaaagacct accggacagg
     4801 cgttaatcaa ccgttgtacc atctgacgct caacggagtc agccggaaca atgtgcggcg
     4861 catcagcaac gcgccagaga ttacgggcta cagacatcgc agactcctga taacatcgcc
     4921 cgccagatgc cacaggccat gacgacgtaa atgatgccag aataactggc gcagcggtgg
     4981 aacaggttta tcaccctgat cccataaatc gcgggaaata tcctgcatta acgcaggcca
     5041 cgcccgatac cagccagggc gttgcaaaag cgccggaaca tgctgataac gttgtagaac
     5101 atcccatagc aggctgcgct ctacgttgtg atgataaagc gcaaacagat tttgcggctc
     5161 gcggtgctgg caggcgctta ttagcgtttg tgccgccgcc tgtgcgccag tcagcgccat
     5221 atccatgccg cgcacggaaa ttccggtatt gacgcaactg cgcaacgcat cgcccaccag
     5281 cagccagccg ttaccggcgt attgcaccgg catactgtgc aagccacctt ctggcaccag
     5341 atgcgcacca tactccagtg attccgtgtt tttgataagc gtgcgcactg ccggatgcgc
     5401 tttaaagcga gtcagcagct cgcttgccgg aacacgactt tgcgtaaggg aagagagcgg
     5461 gcaaacaatc cctaacgaga gcgtttgttg attagtataa agaaatgcgc cgccgggtaa
     5521 gtcatcacag attccgccgc tgaacagcaa cgctgcgcct tcgttattct ccagatgaaa
     5581 acgttcttca atagcggatg tttccagcga cagcacttct ttgatcccca acgccatcgc
     5641 ttcgccagca ggacgagtca ctaacccgtg acgttcagcc aggacgctgt tggcaccttc
     5701 tgccagcacc acataacggg cgcggagaat atcgtcacca caaataacgc cacagactct
     5761 gccgttttct tcatacagtg catccaccgt cgctccgggg atgcattcga caccttcttt
     5821 ttcggcttcg gcaaccagcc acggatcgaa ccgtgcacgt aatacactcc aggattcacc
     5881 gccgggctgt aagctggaaa acgtcgttgc gccatccggc gttaacaggg aaaggctttc
     5941 gtgagtgatg cgtcgttcaa gaggcgcggt cagatgaaat tgcgggagga gttccgcgag
     6001 tgcatgggta tataaccgcc tgccggaaag atttttgctg ccggggattt cagcgcgttc
     6061 gagtaacaaa acggataaat ccgctcgcgc gcagcgtaac gcgcaagcgg tgcctgcaat
     6121 accggcacca ataataataa tgtcgcagtc gtcttccatg tcatgcccca gatatccaga
     6181 acacaacacc ctaacatagc gttactaaag ggaaattgac cgccgacaca ctcttatcat
     6241 tcgccgcgat agatacaccc tgcggtgcag gtttctttta ccatcaccgc acttaacagc
     6301 ggcacaacgg gtttaacctg atcccaaatc cattttgcta aaacttcgct ggttggattt
     6361 tccagacctg gaatatcatt gagataatgg tgatcgaggc gctcataagt tggcttaaac
     6421 gccgctttca gttcagcgaa atcgataatc cagcccgtat gcggatcgac ttccccggta
     6481 atttccagtc gcaccataaa ggaatgcccg tgcaggcgac cacatttatg cccttccggg
     6541 acatgtggta agcggtgagc ggcttcgaag gtgaaatctt taaataacgt ggtggacatc
     6601 ataatttctc tacaggacga taaaaccgcc gtagagtacc ggaaaggctg ttttttcgca
     6661 ttatctaaaa cgactttgtt gcgcaaaatc gctgatttat cttaatgatt ggctaaattc
     6721 atttgttttt cattaggttg gttaatctat tttgttgtta aagactattg ctaaaacagg
     6781 ttagtcgatt tggttattag ttatcgctat cccgtcttta atccacaccg ttcaccccgt
     6841 taaccttacc ttctcttctg ttttatgggc gctgacaggg cgcagaaaca gctttgctta
     6901 ctggaacata acgacgcatg acgacacagg tcccaccttc cgcgttgctt ccgttgaacc
     6961 cggagcaact ggcacgcctt caggcggcca cgaccgattt aacgcctacc cagcttgcct
     7021 gggtttctgg ctatttctgg ggcgtgctca accagcagcc tgttgcgctt gcagtgacgc
     7081 cagcgcccgc cgcagaaatg ccgggtataa cgattatctc tgcttcgcaa accggcaatg
     7141 cgcgccgggt tgctgaagca ttacgcgatg atttattagc agcaaaactg aacgttaagc
     7201 tggtgaacgc gggcgactat aaattcaaac aaatcgccag cgaaaaactg ctcatcgtag
     7261 tgacgtcaac gcaaggggaa ggggaaccgc cggaagaagc cgtcgcgctg cataagttcc
     7321 tgttctccaa aaaagcgcca aagctggaaa acaccgcgtt tgccgtgttt agcctcggcg
     7381 atagctctta tgaatttttc tgccagtccg ggaaagattt tgacagcaag ctggcggaac
     7441 tggggggtga acgcctgctc gaccgtgtcg attctgatgt tgaataccag gctgctgcca
     7501 gcgagtggcg cgcccgcgtg gttgatgcgc ttaaatcgcg tgcgcctgtc gcggcacctt
     7561 cgcaatccgt cgctactggc gcggtaaatg aaatccacac cagcccgtac agcaaagacg
     7621 cgccgctggt ggctagcctc tctgttaacc agaaaattac tgggcgtaac tctgaaaaag
     7681 acgttcgcca tatcgaaatt gacttaggcg actcgggcct gcgttaccag ccgggtgacg
     7741 cgctgggcgt ctggtatcag aacgatccgg cactggtgaa agaacttgtc gaactgctgt
     7801 ggctgacagg cgatgaacct gtcactgtcg agggcaaaac gttgcctctg aacgaagcgc
     7861 tacagtggca cttcgaactg accgtcaaca ccgccaacat tgttgagaat tacgccacgc
     7921 ttacccgcag tgaaacactg ctgccgctgg tgggcgataa agcgaagtta cagcattacg
     7981 ccgcgacgac gctgattgtt gacatggtgc gtttctcccc ggcacagctt gatgccgaag
     8041 cgctaattaa tctgctgcgc ccgctgaccc cgcgtctcta ttccattgcc tcctcgcagg
     8101 cggaagtcga gaacgaagta cacgtcaccg ttggtgtggt gcgttacgac gtggaaggcc
     8161 gcgcccgtgc cggtggtgcc tccagcttcc tcgctgaccg cgtggaagaa gagggcgaag
     8221 tccgcgtatt tatcgaacat aacgataact tccgcctgcc cgctaacctg gaaaccccgg
     8281 tgattatgat tggcccaggc accggtattg cgccgttccg cgcctttatg cagcaacgcg
     8341 ccgctgacga agcgccaggt aaaaactggc tgttctttgg caatccgcat tttacggaag
     8401 acttcctgta tcaggtggag tggcagcgct acgtcaaaga gggtgtgctg acgcggatcg
     8461 atctcgcctg gtcgcgcgac caaaaagaaa aagtttacgt acaagataaa ctgcgcgaac
     8521 agggcgcgga gctgtggcgc tggatcaatg acggtgccca catttatgtc tgcggcgacg
     8581 ctaatcgcat ggcgaaagac gttgagcagg cacttctgga agtgattgcc gaatttggtg
     8641 gcatggacac cgaagcggcg gatgaatttt taagtgagct gcgcgtagag cgccgttatc
     8701 agcgagatgt ctactaatga gcgaaaaaca tccagggcct ttagtggtcg aaggaaaact
     8761 gacagacgcc gagcgcatga agcttgaaag caactacctg cgcggcacca ttgcggaaga
     8821 tttaaacgac ggtctgaccg gcggctttaa gggcgataac ttcctgctga tccgcttcca
     8881 cggcatgtat cagcaggatg accgcgacat ccgcgccgaa cgtgctgaac agaagctgga
     8941 gccgcgccac gcgatgctgt tgcgctgccg tctgccgggt ggagttatca ccaccaaaca
     9001 gtggcaggcg atcgataaat ttgccggtga aaacaccatc tatggcagca ttcgcctgac
     9061 caaccgccag acgtttcagt tccacggcat tctgaaaaag aacgtcaaac cggtgcacca
     9121 gatgctgcac tcggtcggtc ttgatgcgct ggcgactgct aacgacatga accgtaacgt
     9181 actctgcacc tcgaaccctt acgagtcgca gctgcacgca gaagcgtacg agtgggcgaa
     9241 gaagatttct gaacatctgc tgccgcgtac ccgcgcgtat gcggagatct ggctcgatca
     9301 ggaaaaagtc gccaccaccg atgaagaacc gatccttggt cagacttacc tgccgcgtaa
     9361 gttcaaaacc acggtagtga tcccgccgca gaacgatatt gatctgcacg ccaacgacat
     9421 gaacttcgtg gcgatcgcgg aaaacggcaa gctggtgggc ttcaacctgc tggtgggcgg
     9481 tgggctttct atcgaacacg gtaacaagaa aacctacgcc cgcacggcga gtgagtttgg
     9541 ctatctgccg ctagagcata cgctagcggt ggcggaagcc gtcgtgacaa ctcagcgtga
     9601 ctggggtaac cgaaccgatc gtaaaaatgc caaaaccaaa tacacactgg agcgcgtggg
     9661 ggttgagacg tttaaagcgg aagtggagcg tcgcgcgggg atcaaatttg aaccgatccg
     9721 cccgtatgaa tttacaggac gcggcgatcg tattggctgg gttaagggca ttgatgataa
     9781 ctggcacctg acgctgttta tcgaaaatgg tcgcatcctt gattatccgg ggcgtccgct
     9841 gaaaactggc ctgctggaga tcgcgaagat ccacaaaggt gatttccgca ttacggcgaa
     9901 ccagaatctg atcatcgccg gtgtgccgga aagcgagaaa gcgaagatcg agaaaattgc
     9961 caaagagagc gggttaatga atgccgtcac gccgcagcgt gaaaactcga tggcctgcgt
    10021 gtcattcccg acttgcccgc tggcgatggc ggaagcggag cgtttcctgc cgtcttttat
    10081 cgacaacatc gataatttaa tggtgaaaca tggtgtcagc gatgagcata tcgtgatgcg
    10141 tgtaacaggc tgcccgaacg gttgtggtcg cgcgatgctg gcggaagtgg gcttggtggg
    10201 taaagcaccg ggtcgctaca acctgcatct tggcggcaac cgcattggga cacgtatccc
    10261 acggatgtat aaagaaaaca tcaccgagcc ggaaatcctg gcgtcgcttg atgaactgat
    10321 agggcgctgg gcgaaagagc gcgaagcggg tgaaggcttc ggcgacttta cggtgcgtgc
    10381 gggcatcatt cgcccggtgc tcgatccggc gcgcgatttg tgggattaac catcagcccg
    10441 gtcttgtagg cctgataaga cgcgtgagcg tcgcatcagg caaggcaaac agtgaggaat
    10501 ctatgtccaa actcgatcta aacgccctga acgaactgcc gaaggtagat cgcattctgg
    10561 cgctggcaga aactaacgcc gaactggaaa aactggacgc tgaaggccgc gttgcctggg
    10621 cgctggataa tctgcccggt gaatatgtac tttcttccag ctttggcatt caggcggcgg
    10681 tgagcctgca tctggtgaat cagattcacc cggatattcc ggtgatcctc accgacaccg
    10741 gctatctgtt cccggaaacc taccgcttta ttgacgagtt aacggacaaa ctcaagctca
    10801 acctgaaagt gtaccgcgct accgaaagcg ccgcctggca ggaagcacgc tacggcaaac
    10861 tgtgggaaca gggcgttgaa ggcattgaaa agtacaatga catcaacaaa gtcgaaccga
    10921 tgaaccgggc gctgaaagaa ctgaatgcgc aaacgtggtt tgctggcctg cggcgtgaac
    10981 aatccggcag ccgtgccaat ttaccggtgc tggcaattca gcgtggcgta tttaaagtgc
    11041 tgccgattat cgactgggat aaccgaacta tttatcagta cctgcaaaaa catggcctga
    11101 aatatcaccc attatgggac gaaggatatt tatcggtggg cgatacccat acaacccgta
    11161 aatgggaacc cggcatggcg gaagaagaaa cgcgtttctt tggcttaaaa agggaatgtg
    11221 gattacacga agggtaaata ttgccggata aattaaacgc ctctgtcaga aatgatgggg
    11281 gcgtttttat tttgcgagca gcattccggc atttaaaggt gaacatcgca gggaaaatta
    11341 acaggtatag aatggcgttg ggtgcttgag gctgtctggc gttaagcatt cgcgaggttc
    11401 cagatggaca aaagccccag gcgatatttc tatcaacctg aggctcgcgt tcgaacgtag
    11461 caattcggat gttagtctct tctttgcagg gaggcaagac atgctgacaa aatatgccct
    11521 tgtggcaatc atcgtactgt gttgtacagt actgggattc acgttgatgg taggtgactc
    11581 gttatgtgag ttgagtatca gagaacgtgg tatggagttt aaggcagttc tcgcttacga
    11641 atcgaagaag tagccaccac gcgggggtaa aaccccgcgt actgaattgt gatggtttgg
    11701 tctcctgcac ccttttttta aatttatatt ttttatgtga atatatagtc tctatttatt
    11761 attatgaatt tcaaatgaaa atcattaatt tattcataat gtaaatgttt tattggctga
    11821 tatatatcat agatagtttt taaaatatgc gtaaatatcc tttaagttta ctgaaggata
    11881 aaaatattgt gactttcttt gatttctggg gaaaaaaccg acgtggcgag aaagagggta
    11941 tcgacgacta tcaccttctt tgctggcatt tgctggatgt ggccgcaatg ggctatttaa
    12001 tggttaaaag aaattgcttc gggctggctg attattttcg tcaattaggg atttctgaca
    12061 aggaacaggc agctcaattt ttcgcctggt tgctgtgctg gcacgatatt ggaaaatttg
    12121 cccgctcttt tcaacaactt tacctgcccc ctgaattcaa gattcaggaa ggtgccagaa
    12181 aaaattacga aaaaatctct cattcaacgc tgggctactg gttgtggaat cattatttaa
    12241 gtgaatgtca ggagttactt ccttcatctt cgctctctcc gcgcaaactt aggcgagtga
    12301 tagaaatgtg gatgcccgtg acgacagggc atcatggtcg gccccctgat cgtatggatg
    12361 aattggataa ttttctgcct gaagacaaag ctgcagcacg agattttctc cttgaaatca
    12421 agccactgtt tccgcgaata gagatcccca cattctggga tgatgacgag ggcgttgaac
    12481 ttataaaaca actttcctgg tatatctctg caaccgtcgt acttgcagac tggacagggt
    12541 cgtcaacgcg attttttcca cgcgtcgcac aggcaatgga tattgaagat tactggcaaa
    12601 aggctttagt tcaggctcaa aacgcattaa ccgtctttcc gccacaagca gaaaccgcgc
    12661 ctttcaccgg aattaatacg ctgtttcctt ttattgagaa tacgacaccg ttacagcaaa
    12721 aggtgctgga tctggatatc aatcagccag gaccacagtt gtttattctg gaagatgtga
    12781 ccggcgcagg taaaacagaa gcggcgctta tcctggcgca caggttgatg gctgcgggta
    12841 acgctcaggg gctgtttttt ggcctgccaa caatggcaac ggccaatgcc atgtacgatc
    12901 gactggtcaa aacctggctt gctttctatt cgccagaggt gttccccgct ccagcggaga
    12961 taaaccggcg caatacggtg cggttttccc ggaagtaaac cggacgcact ggatgcgatg
    13021 atgggtatca cttagaattc cccgcccctg cggtagaact cccagctccc attttcaaac
    13081 ccatcaagac gccttcgcca gctccttcac caacggtagc attatccgca taacgtcacg
    13141 gcagcgacgt tctattcttc caggaagtgc cttatcaata tgttgttgat tatccagtct
    13201 tacgtcatgc cagctatttc ccgccgggaa ggcaggtgtt tttgcgcgtt gctgataacc
    13261 atccttattt ccaagattcc agttagtcgc ttccaccgaa agtacagcaa tgcccgcttt
    13321 gtcgaatatt tctgcgtcat tacaacaccc agtgcctttc ggataatttt tattcaaacc
    13381 cggattggtc gttgcggcta ttccgtgact gcgtgcaatt gccagcgccc tgtcgcgcgt
    13441 taatttcctt actgcttcag gggtttttac accgctgttg aaatacaatt tatcgccaac
    13501 aattaagtta tcgagattaa tcaccagcag cgtatttttc ttttcggtgt cactcatccg
    13561 cttgagtaaa ttctcagcgc ctaattttcc ttcctcttcg ccgctggtcg ccacaaatcg
    13621 aataccatac tcggtaggcg tatttttcag gcgttctgcc agttccagca tgacacctaa
    13681 acctgcggcg ttatcatcta ttccttgtaa cgtcagtccg ccgagattgg catctgcgtc
    13741 agcatcgctc agcggggcgt aagtatccag atgcgccata atgatgatct gctgcggcgc
    13801 tttgccttca tgagcggcaa tcaccgtact tcctgtcacg ttatgccagt tcttgcgatt
    13861 atcgcgggcg gtataaatat accgactatt aaatgtccga atatcactgc gataacccat
    13921 ttgctgaaac tgttggcgaa tataatcggc agataacatt tctgctggag ttccggtcat
    13981 gcgtcccgga aagaaagtag caatatgtcg tgcctgggta ttagcaaaat cgccaggttt
    14041 aggtgacgag gcgtgtacgg ggagaataaa gcatacgccg agcgccaggg cagcggtacg
    14101 gtggcgcaat gcggaaaaca tagtgagtcc ttaaatacca tgcaaatttt tttaccgcca
    14161 tagtatgaaa ctgccgctgc gctaaaacaa tttcaaatct tcctaaacgc ccgaaatccg
    14221 gtgccttaag cactttttga tattagcttt gccaaatcgt tattccgtta aggaactact
    14281 cattctaatt ggtaatttca ttcgttctct tacgctccct atagtcgaaa catctgatgg
    14341 caagaaaata gcggtattgc aaaggaacgg ttatggatca aaaacgactt actcacctgc
    14401 ggcaactgga ggcggaaagc atccacatta ttcgcgaggt ggcggcagaa ttctcaaatc
    14461 cggtgatgct ctactctatc ggtaaagatt ccagcgtcat gctgcatctg gcgcgcaagg
    14521 cgttttatcc aggtacgctg cctttcccgt tgctgcatgt cgataccggc tggaaattcc
    14581 gcgagatgta tgagttccgc gatcgtacgg cgaaagccta cggctgcgag ctgctggtgc
    14641 ataaaaaccc ggaaggcgtg gctatgggga ttaatccatt cgtgcacggc agcgcgaaac
    14701 ataccgatat tatgaaaact gaaggcctga aacaggcgct gaacaaatac ggttttgatg
    14761 ccgccttcgg tggtgcgcgc cgtgacgaag agaaatcccg cgctaaagag cgaatctact
    14821 ctttccgtga ccgcttccat cgctgggatc cgaaaaatca gcgcccggag ctgtggcaca
    14881 actacaacgg gcaaattaac aaaggcgaaa gcatccgcgt cttcccgctc tctaactgga
    14941 ccgagcagga tatctggcaa tacatctggc tggaaaatat cgacattgtg ccgctgtatc
    15001 tcgctgcgga acgtccggtt ctggaacgcg acggtatgtt gatgatgatt gatgacaacc
    15061 gtatcgacct gcaaccgggc gaagtgatca aaaaacggat ggtgcgtttc cgtacgctgg
    15121 gctgctggcc gctgaccggt gcggtggagt caaatgcaca aacactgccg gaaatcatcg
    15181 aagagatgct ggtttccacc accagtgaac gtcaggggcg cgtgattgac cgcgaccagg
    15241 cgggatccat ggagctgaaa aaacgtcagg ggtattttta agatgaacac cgtacttgca
    15301 caacaaatcg ccaatgaagg cggcgtcgaa gcctggatga ttgcgcaaca acataaaagc
    15361 ctgctgcgtt ttctgacctg tggtagcgtc gatgacggca aaagtaccct gattggtcgt
    15421 ctgctgcacg atacccgcca aatctacgaa gatcagctct catcgctgca taacgacagt
    15481 aagcgtcacg gcacccaggg cgaaaagctg gatctggctc tgctggtgga cggcctgcaa
    15541 gctgagcgcg aacagggcat cactattgat gtggcctacc gctatttctc taccgagaag
    15601 cgtaaattta ttatcgccga caccccaggg cacgagcagt acactcgcaa tatggcgact
    15661 ggcgcatcga catgtgaact ggcgatctta ctgattgatg cccgtaaagg cgtgctcgat
    15721 caaacccgtc gtcacagttt tatctccaca ctgttgggga tcaaacatct ggtcgtggcg
    15781 atcaacaaaa tggatctggt ggattacagt gaagagacgt tcacccgtat tcgtgaagat
    15841 tatctgacct ttgccgggca gctgccggat aatctggata tccgctttgt gccgctctcc
    15901 gcactggaag gtgacaacgt ggcttcgcaa agtgaaagta tgccgtggta cagcggtctg
    15961 acactgctcg aagtgctgga aaccgtggag atccagcgag tggtggatgc ccagccaatg
    16021 cgcttcccgg tgcagtacgt taaccgccca aatctcgatt ttcgtggcta cgccggaatg
    16081 ctggcatccg gtcgcgtgga agtcgggcaa cgtgtaaaag tgctgccctc tggtgtggaa
    16141 tcaaacgtcg cgcggatcgt gacttttgat ggtgatcgcg aagaagtctt tgccggagaa
    16201 gcgatcaccc tggtgctgac ggatgagatc gatatcagcc gtggtgatct gctgctggcg
    16261 gcagacgaag cgttaccggc ggtgcagagt gcgtcggtgg atgtggtatg gatggcggaa
    16321 cagccgcttt ccccgggcca gagttatgac atcaaaattg ccggtaagaa gacgcgtgct
    16381 cgtgttgatg gcattcgtta tcaggttgat attaataacc ttacccaacg cgaagttgaa
    16441 aacctgccgc tgaacggcat cggcctggtg gatctcactt ttgacgagcc actggtgtta
    16501 gatcgctatc agcaaaatcc ggttaccggt gggctgattt ttatcgatcg cctgagcaat
    16561 gtgaccgtgg gtgccggtat ggtgcacgag ccagttagcc aggcaacagc tgcgtcatct
    16621 gaattcagtg cattcgaact ggaattgaat gccctggttc gtcgccactt tccgcactgg
    16681 ggcgcgcgcg atttgctggg ggataaataa tggcgctgca tgacgaaaac gtcgtctggc
    16741 atagtcatcc ggtcactgtg caacaacgcg aactacacca cggtcatcgt ggtgtagtgc
    16801 tgtggtttac cggcctctcc gggtccggta aatcaacggt cgccggggcg ctggaggagg
    16861 cgttacataa actcagcgtc agtacgtatc tgctggatgg cgacaatgtt cgccacggat
    16921 tatgcagcga tctcggtttt agcgatgccg atcgtaaaga gaatatccgt cgcgtcggtg
    16981 aagtggcgaa tttgatggtt gaagccggac tggtggtgct gaccgcattt atctcgccac
    17041 accgcgccga acgccagatg gttcgcgaac gcgtaggaga agggcgcttt attgaagtgt
    17101 ttgtcgatac gccgctggcg atttgcgaag cccgcgatcc aaaaggctta tataagaaag
    17161 cgcgtgccgg tgaactgcgc aactttacgg gaatagattc cgtttacgaa gcgcctgaat
    17221 cggcagaaat tcatctcaat ggtgaacaat tagtaacaaa tttggtacag caattattag
    17281 atctgttgag acagaacgat attatcagat cctgagacac caccgggttt ccatgcccgg
    17341 ttcgtcaaag taacaggatc aaatatgcgt aatagccata acattacact aacaaataac
    17401 gacagcctta ctgaggatga agaaactaca tggtcactgc ctggtgccgt ggtcggtttt
    17461 atctcctggt tatttgcgct ggcgatgccg atgttgattt atggctctaa cacgctgttc
    17521 ttctttctct acacctggcc tttctttctg gcactgatgc ctgtcgcggt agtggtgggg
    17581 attgcgctgc attcattgat ggacggaaag ctacgctaca gtattgtttt caccctggtg
    17641 acagtaggca ttatgtttgg ctcactgttt atgtggctac tgggctaatt tgtactttct
    17701 tgtctcctct gttcataatt caaaccgtaa ctaataatga gattatgttc tgcacgccct
    17761 gggtatacgt aacaatggac aaatgtggta catttgcccg cgttgtcgcg gtatccccaa
    17821 cagaggatgt agagtcgtct tcggatgcat gggatgatga tgcagttttt cagggggcag
    17881 gatgggtaaa ctaacgctgc tgttgctggc tattctggtc tggctacagt attcgctgtg
    17941 gttcggtaag aacggtatac atgactatac ccgcgtcaat gatgatgtgg cggcactgca
    18001 agctacaaac gcgaaactta aagcgcgaaa cgatcaactt tttgccgaaa ttgacgatct
    18061 caatggcggc caggaggcgc tcgaagagcg tgcgcgtaat gaactcagca tgaccaggcc
    18121 gggcgaaact ttttatcgtc tggtgcctga cgcgtcgaag cgcgcacagt ctgcggggca
    18181 aaacaatcga taaatcagcc cgggaattaa catggcaacc actcatttgg atgtttgcgc
    18241 cgtggttccg gcggccggat ttggccgtcg aatgcaaacg gaatgtccta agcaatatct
    18301 ctcaatcggt aatcaaacca ttcttgaaca ctcggtgcat gcgctgctgg cgcatccccg
    18361 ggtgacacgt gtcgtcattg ccataagtcc tggcgatagc cgttttgcac aacttcctct
    18421 ggcgaatcat ccgcaaatca ccgttgtaga tggcggtgat gagcgtgccg attccgtgct
    18481 ggcaggtctg aaagccgctg gcgatgtgca gtgggtattg gtgcatgacg ccgctcgtcc
    18541 ttgtttgcat caggatgacc tcgcgcgatt gttggcgttg agcgaaacca gccgcacggg
    18601 agggatcctc gccgcaccag tgcgcgatac tatgaaacgt gccgaaccgg gcaaaaatgc
    18661 cattgctcat accgttgatc gcaacggctt atggcacgcg ctgacgccgc aatttttccc
    18721 tcgtgagctg ttacatgact gtctgacgcg cgctctaaat gaaggcgcta ccattaccga
    18781 cgaagcctcg gcgctggaat attgcggatt tcatccgcag ttggtcgaag gccgcgcgga
    18841 taacattaaa gtcacgcgcc cggaagattt ggcactggcc gagttttacc tcacccgaac
    18901 catccatcag gagaatacat aatgcgaatt ggacacggtt ttgacgtaca cgcctttggc
    18961 ggtgaaggcc caattatcat tggtggcgta cgcattcctt acgaaaaagg attgctggcg
    19021 cattctgatg gcgacgtggc gcttcatgcg ttgaccgatg cattgcttgg cgcggcggcg
    19081 ctgggggata tcggcaaact gttcccggat accgatccgg catttaaagg tgccgacagc
    19141 cgcgagctgc tacgcgaagc ctggcgtcgt attcaggcga agggttatac ccttggcaac
    19201 gtcgatgtca ctatcatcgc tcaggcaccg aagatgttgc cgcacattcc acaaatgcgc
    19261 gtgtttattg ccgaagatct cggctgccat atggatgatg ttaacgtgaa agccactact
    19321 acggaaaaac ttggatttac cggacgtggg gaagggattg cctgtgaagc ggtggcgcta
    19381 ctcattaagg caacaaaatg attgagtttg ataatctcac ttacctccac ggtaaaccgc
    19441 aaggcaccgg gctgctgaaa gccaatccgg aagactttgt ggtggtggaa gatttgggct
    19501 ttgagcctga tggtgaaggt gagcatattc tggttagaat cctcaaaaac ggctgcaata
    19561 cccgttttgt ggcggatgca ctggcgaaat tcctgaaaat tcattcccgt gaagtcagct
    19621 tcgctgggca aaaagacaaa catgctgtaa cggaacagtg gttatgtgct cgcgtgccgg
    19681 gcaaggaaat gcccgatctg agcgcctttc aactggaagg ctgccaggtg ctggagtatg
    19741 cgcggcacaa gcgcaagctg cgtttagggg cgctgaaagg taacgccttt accctggttc
    19801 tgcgcgaagt gagcaatcgc gatgacgttg aacaacgtct gatcgatatt tgcgtaaaag
    19861 gtgtaccgaa ctacttcggt gcccaacgtt ttgggattgg cggtagcaac ttgcagggcg
    19921 cgctgcgctg ggcgcaaacc aatactccgg tgcgcgatcg caataaacgg agtttttggt
    19981 tgtcggcagc ccgcagtgcg ttgtttaatc agattgttgc tgagcgcctc aaaaaagcag
    20041 acgttaatca agttgttgac ggcgatgcgc tacaattagc cggacgtggt agctggtttg
    20101 tagcaaccac cgaagaactg gcggaattac agcgtcgcgt caacgataaa gagctgatga
    20161 taaccgccgc attgccgggc agtggcgaat ggggaactca gcgtgaagcg ctggcattcg
    20221 aacaagcagc tgtcgccgca gaaactgaat tacaagcttt actggtgcgc gaaaaagttg
    20281 aagccgcgcg cagagcgatg ctgctgtatc cgcaacaatt aagctggaac tggtgggatg
    20341 acgtcaccgt agagatccgt ttctggcttc cggcgggtag ttttgcaacc agcgttgtca
    20401 gggaacttat caacacaaca ggtgattatg cgcatattgc tgagtaatga tgacggggta
    20461 catgcacccg gtatacaaac gctggcgaaa gccttgcgtg agtttgctga cgttcaggtg
    20521 gtcgcccccg atcgtaaccg cagcggcgct tcaaattctc tgacactgga atcctccctg
    20581 cgcacgttta cctttgaaaa tggtgatatt gctgtgcaaa tgggaacccc gaccgattgt
    20641 gtctatcttg gcgtgaatgc tctgatgcgt ccgcgcccgg acattgttgt gtccggaatt
    20701 aacgccgggc cgaatctggg ggatgatgtt atttattccg gtacggtagc cgccgcgatg
    20761 gaaggccgtc atttaggttt tccggcgctg gccgtctcgc ttgacgggca taaacattac
    20821 gacactgccg cggcggtaac ctgttcaatt ttgcgcgcac tgtgtaaaga gccgctgcgc
    20881 accggacgta ttcttaatat taacgttcag gatttaccct tggatcaaat caaaggtatt
    20941 cgcgtgacgc gctgcggtac acgacatccg gcagagcagg tgatcccgca gcaagatccg
    21001 cgcggcaata cgctgtactg gattggcccg ccgggcggta aatgtgatgc cggtccgggg
    21061 accgattttg ctgcggtaga tgagggctat gtctccatca cgccgctgca tgtggattta
    21121 actgcgcata gcgcgcaaga tgtggtttca gactggttaa acagcgtggg agttggcacg
    21181 caatggtaag cagacgcgta caagcacttc tggatcaatt acgtgcgcaa ggtattcagg
    21241 atgagcaggt gctgaatgca cttgccgccg tgccgcgtga aaaattcgtt gatgaagcgt
    21301 ttgaacaaaa agcctgggac aatatcgcct tgccgatagg tcaggggcag acaatttcgc
    21361 agccgtatat ggtggcgcga atgaccgaat tactcgagct gacgccgcag tcgcgggtgc
    21421 tggaaattgg cactggttcg ggatatcaaa cggcaatcct ggcgcatctt gtccagcatg
    21481 tttgctcggt tgaacggatt aaaggcttgc agtggcaggc acgtcgccgc ctgaaaaatc
    21541 ttgatttaca taatgtttca acccgtcatg gtgatggatg gcaaggttgg caggcacgtg
    21601 cgccgtttga cgctatcatt gttacggcgg caccgccgga aattccaact gcgctaatga
    21661 cgcagctgga cgaaggcggg attctcgtct tacccgtagg ggaggagcac cagtatttga
    21721 aacgggtgcg tcgtcgggga ggcgaattta ttatcgatac cgtggaggcc gtgcgctttg
    21781 tccctttagt gaagggtgag ctggcttaaa acgtgaggaa atacctggat ttttcctggt
    21841 tattttgccg taggtcagcg tatcgtgaac atcttttcca gtgttcagta gggtgccttg
    21901 cacggtaatt atgtcactgg ttattaacca atttttcctg ggggataaat gagcgcggga
    21961 agcccaaaat tcaccgttcg ccgcattgcg gctttgtcac tggtttcgct atggctggca
    22021 ggctgttctg acacttcaaa tccaccggca ccggtcagct ccgttaatgg caatgcgcct
    22081 gcaaatacta attctggtat gttgattacg ccgccgccga aaatggggac gatgtctaca
    22141 gcgcagcaac cgcaaattca gccggtacag cagccacaaa ttcaggctac tcaacaaccg
    22201 caaatccagc cagtgcagcc agtagctcag cagccggtac agatggaaaa cggacgcatc
    22261 gtctataacc gtcagtatgg gaacattccg aaaggcagtt atagcggcag tacctatacc
    22321 gtgaaaaaag gcgacacact tttctatatc gcctggatta ctggcaacga tttccgtgac
    22381 cttgctcagc gcaacaatat tcaggcacca tacgcgctga acgttggtca gaccttgcag
    22441 gtgggtaatg cttccggtac gccaatcact ggcggaaatg ccattaccca ggccgacgca
    22501 gcagagcaag gagttgtgat caagcctgca caaaattcca ccgttgctgt tgcgtcgcaa
    22561 ccgacaatta cgtattctga gtcttcgggt gaacagagtg ctaacaaaat gttgccgaac
    22621 aacaagccaa ctgcgaccac ggtcacagcg cctgtaacgg taccaacagc aagcacaacc
    22681 gagccgactg tcagcagtac atcaaccagt acgcctatct ccacctggcg ctggccgact
    22741 gagggcaaag tgatcgaaac ctttggcgct tctgaggggg gcaacaaggg gattgatatc
    22801 gcaggcagca aaggacaggc aattatcgcg accgcagatg gccgcgttgt ttatgctggt
    22861 aacgcgctgc gcggctacgg taatctgatt atcatcaaac ataatgatga ttacctgagt
    22921 gcctacgccc ataacgacac aatgctggtc cgggaacaac aagaagtgaa ggcggggcaa
    22981 aaaatagcaa ccatgggtag caccggaacc agttcaacac gcttgcattt tgaaattcgt
    23041 tacaagggga aatccgtaaa cccgctgcgt tatttgccgc agcgataaat cggcggaacc
    23101 aggcttttgc ttgaatgttc cgtcaaggga tcacgggtag gagccacctt atgagtcaga
    23161 atacgctgaa agttcatgat ttaaatgaag atgcggaatt tgatgagaac ggagttgagg
    23221 tttttgacga aaaggcctta gtagaagagg aacccagtga taacgatttg gccgaagagg
    23281 aactgttatc gcagggagcc acacagcgtg tgttggacgc gactcagctt taccttggtg
    23341 agattggtta ttcaccactg ttaacggccg aagaagaagt ttattttgcg cgtcgcgcac
    23401 tgcgtggaga tgtcgcctct cgccgccgga tgatcgagag taacttgcgt ctggtggtaa
    23461 aaattgcccg ccgttatggc aatcgtggtc tggcgttgct ggaccttatc gaagagggca
    23521 acttggggct gatccgcgcg gtagagaagt ttgacccgga acgtggtttc cgcttctcaa
    23581 catacgcaac ctggtggatt cgccagacga ttgaacgggc gattatgaac caaacccgta
    23641 ctattcgttt gccgattcac atcgtaaagg agctgaacgt ttacctgcga accgcacgtg
    23701 agttgtccca taagctggac catgaaccaa gtgcggaaga gatcgcagag caactggata
    23761 agccagttga tgacgtcagc cgtatgcttc gtcttaacga gcgcattacc tcggtagaca
    23821 ccccgctggg tggtgattcc gaaaaagcgt tgctggacat cctggccgat gaaaaagaga
    23881 acggtccgga agataccacg caagatgacg atatgaagca gagcatcgtc aaatggctgt
    23941 tcgagctgaa cgccaaacag cgtgaagtac tggcacgtcg attcggtttg ctggggtatg
    24001 aagcggcaac actggaagat gtaggtcgtg aaattggcct cacccgtgaa cgtgttcgcc
    24061 agattcaggt tgaaggcctg cgccgtttgc gcgaaatcct gcaaacgcag gggctgaata
    24121 tcgaagcgct gttccgcaag taagtaagca tctgtcagaa aggccagtct caagcgaggc
    24181 tggccttttt cttttgtttg ggaagcttgt gtttttgtcg cctggataag acacgtcagc
    24241 gtcctacccg gcaatcagca ttctatgtgc tcatcatctt gcgcaccaac tgcataaaca
    24301 gctcctgctc ttctccactc aaacgcccca aaaattcctc atccacgctg tcaccaatag
    24361 gtatcgccgc agcaagtagc ttttctcctt cggcagtcag ccagacaaag cgtcgccgct
    24421 tgtcagcgac gtcatgttca cgtctgacta agccacgatt ttccattctt gccaacattt
    24481 ctgctagcgt tgctttggtg ctgactgctg cttctatcag tgccacctgt tcaataccag
    24541 gtttatcagc aatggcacgc atcaccgcgt actgtggttt ggtcaggtca ggtaactcgt
    24601 gctgccagcg agccgtgtgc tgctgaaaaa gctgtcgtaa ctgatggaac gctttatttc
    24661 gtaacgccat gtaaactccg ggctaattct ctgtcgctat catagcggtt ttttagtgac
    24721 gaagaagatg ttgttacttt tcaatccttg ccgctgtgga aatgaaggag tatgttaata
    24781 ataacgttcg tatacgaata attaagagaa taagcaatga aactgatcgt cgggatgaca
    24841 ggggctaccg gtgcgcctct tggtgtggca ttactgcaag cgctgcgtga gatgccgaat
    24901 gtcgagactc atctggtgat gtcgaagtgg gcgaaaacca ccattgaact ggaaacgcct
    24961 tacagcgctc gcgatgttgc tgccctcgca gacttcagcc ataacccggc ggatcaggcg
    25021 gcgatcatct catccggttc ttttcgtacc gacggcatga tcgttattcc gtgcagtatg
    25081 aaaacgctcg ccggtatccg cgctggttac gccgatggcc tggtagggcg cgcggcggac
    25141 gtcgtgctca aagaaggccg caaactggtg ctggtgccgc gtgaaatgcc gcttagcacc
    25201 atccatctcg aaaatatgct cgcactttca cgcatgggcg tggcgatggt gccgccgatg
    25261 cctgcctttt ataaccatcc cgaaacggta gatgacattg tccaccatgt ggtagcccgc
    25321 gtgctggatc aatttggcct cgaacatccc cacgccaggc gctggcaagg attaccgcag
    25381 gcccggaatt tttctcagga gaatgaataa tggcatttga tgatttacgc agctttttac
    25441 aggcgcttga tgaccacggc cagttactga aaatcagcga agaagtgaac gctgagccgg
    25501 atctggcagc agcagctaac gccaccgggc gtatcggcga cggcgcgccc gcgctgtggt
    25561 ttgataatat tcgcggcttt accgatgccc gcgtggcgat gaataccatc ggttcctggc
    25621 agaaccacgc gatttccctt ggcctgccac caaacacccc ggtcaaaaag cagattgatg
    25681 agtttatccg ccgctgggat aacttcccga ttgccccgga gcgccgcgcc aatccagcct
    25741 gggcgcagaa caccgtcgat ggcgaagaga tcaacctgtt cgatattctg ccgctgtttc
    25801 gcctgaatga tggcgatggc ggtttctatc tcgacaaagc gtgcgtggtt tcccgcgatc
    25861 cgctcgaccc ggataacttc ggcaagcaga acgtcggcat ctaccgcatg gaagtgaagg
    25921 gcaagcgtaa gctcggcctg caaccggtgc cgatgcacga tatcgccctg catctgcata
    25981 aagcagaaga gcgcggtgaa gatctgccga ttgcgatcac gctcggtaac gatccgatca
    26041 tcacgctgat gggggccacg ccgctgaaat acgatcagtc tgagtatgaa atggcaggcg
    26101 cgctgcgtga aagcccgtac ccgatcgcca ccgctccgct gaccggtttt gatgtgccgt
    26161 ggggttcaga agtgatcctc gaaggggtta tcgaaagccg taaacgcgaa atcgaagggc
    26221 cgttcggtga gtttaccggg cactactccg gtgggcgcaa catgaccgtg gtgcgcatcg
    26281 ataaagtctc ttatcgcagc aaaccgattt tcgaatcact ctatctcggt atgccgtgga
    26341 ccgaaatcga ctacctatga ctaggtaatt tattcaagat aaccgggaag agattaaaaa
    26401 agacgggaat agattggaat caacaagtta ggaactcaag gttttcaaga taaagcgagt
    26461 ccaaaaatgt taaacggttc gatatcaata aaacattcaa gataactcac aggttcttac
    26521 aatagccttt aaagaccgtt caaaaacgat tcaggtgttt attatagcgc tgatgggtgc
    26581 aagcgccaaa accaatgtaa ttttagcaca gctaggcact tgcactgggt tatgcgctgg
    26641 ctgctttctg aggtagattt aagtgatcgc ccttagcttc ggacaacgcg taaatctctc
    26701 taaccttttc gtctggcaaa ttaccaaggc ttaacgctat ggctctacga actccaacta
    26761 gagcgtgcaa ctcttggatg tgctcatcca gaagaatgag taattgctcc gctccttttc
    26821 tcccaagaac atcagctact tgttgttttt cttgtggttt aagaaatgac aagaacatta
    26881 gaatttgagc atcaacatta tccgcattct cttttttggg ttctttggaa tcttttagtt
    26941 caacatcacc ctctcccgtc atgagccatt ccaaggtaac attttcgctt tctgctatct
    27001 tttttaactg atgtgggcgt ggtatcggtg cgtaatcccg cctgttgtaa taatttttta
    27061 aagtgccttc attgatcccc catgcccgag cagccgcatt tcttgaaggg tatcgttcta
    27121 ctaacctaaa gattctttcg ataatcggtt cttttccatc ggcaggaaaa gaaccttggg
    27181 gtgcttttct cattcttggt tcctttctat ctaattcatt gataaataat aaaattaaaa
    27241 aataaaccat aaaaacacca aagaaccctt tacagggaat ctttggtatc ttaatcttta
    27301 tccatacgga tatccgaccg gatatccgcg cgggtaattt tgtaggttaa aggaaagatg
    27361 agacaaagag aagatagtga ccgcgattgg ccttcgctga agattcagct ggcgctcaaa
    27421 gataacgggt ggtcttcctt ccgggagctt tcactccatg ctggttatgc tcaggattca
    27481 ctgaaagcgg tagttcgcac accaaacaaa aaatacgaac gcgtaatttc tgacgctata
    27541 ggtgtaccgc cagaaatcat ctggcccagc cggtatcgta acgatggatt tatcaggaag
    27601 gcgtcgtgat atgcagtcgt acgttaccgt aaatgacctg ctcggcgtac caggaatgcc
    27661 agccaccaca aaaggcatcc gacaggcgtt acagcgtttt tctcgtgatc ttggtgacgt
    27721 tagtcgccgt cgggaaggca caaaagccat tgagtaccac attgattgtc tgcctgaaat
    27781 aacacgaaag gcactgcgtg aacgctatgt ggaacagctg gtggctacag agaacaatgt
    27841 ttctgaagtg aaagctgtta ccagaaaaac acgcaatcct gacgctgtcc aggcaatcga
    27901 agcatatcgc ggttctccac aactgatgga agaacgcctg aatgcgctga ccgaaaacca
    27961 gcgctgggta tctgaagcaa gagctgcgct ggtggtggaa gtgctgaagc tggaaagcgc
    28021 cggtaacccc gggcgactga aagccattaa ctttcttgtt gaaaaagccc gtaaaggtga
    28081 gctgccggag cgcctgcaac aggccgcagt taacgccaat gcaaaacgtg gcgctaatcg
    28141 tactatcagc cgcgatccac tttaccagtg ggttctgaaa tacaaccagt cgcagaacgc
    28201 cgccgaacgg ctgctgttac tggcaccggg aaaacgcgac gaaataaaac cggaagaaat
    28261 cagctggctg cctgaatttc tggcgcagta tcgccaggtg aacggcagac ccatgtcaga
    28321 agcctacgag gattttgtcg cagaatggca gcgtcgccat gcggacgaac cgtacatgct
    28381 ggaggtgatg ccttcctatg atgttgtccg gtatgccatg aaaaaactgc cggaagtggt
    28441 gaaacaaaaa gggcgcgtta ccggcagtga ataccgtcag cttgaaggat tcacccgccg
    28501 cgactggaca gctatgccgg tgaattatgt ctggattggt gacggtcacg gtatgaagct
    28561 gaaatgtgcg catccgatcc acgggcgtcc attctcaccg gaagtcacgt ttgtgatcga
    28621 tggtggaaca cggtttgttg ttggctggag tcttgacctt gccgaaaacg tgttcgccgt
    28681 tgccggtgcg atacagcacg gcattcgcaa ccacggaaaa ccgttcctgt attactcgga
    28741 taacggttcc ggtgaaacgg ctgacatgct ggacaaagag gtcgtgggta ttctgccacg
    28801 cctggggatt aaacacccta ccggtattgc cggtaatcca cagggacgcg gcattattga
    28861 acgcctgaac cggacactgc cgatgcgtat agccaggagg tatcgcacct attttggcaa
    28921 gggtgctgac cgggaatcgt tgcgtgtact taaccgtgat ctgcgttcag cgtttaacgc
    28981 cctgcaacag gacaagcctc tgaacgaccg ccagaaagcg gcaatgcgcg agctgccatc
    29041 gtgggcggag cttattgagg ctattcgtga aggcgtggaa tggtacaaca accgcccgca
    29101 ttctgagctg ccgatgaaac cgaacggacg gcattacagc ccgacagagt tcagaaaaaa
    29161 acgccaggca gaagaagaca ccgaaattga gtggctgagt gatcttgagc tgcgcgacat
    29221 gttccgccca atggtggaac gaccggtaag acgctgcgaa attcagtggc tgaacaatat
    29281 ttactacgcg ccggagttgc gtgacgaaca cggtcgcaaa gtgcttatca gttatgacat
    29341 tcatgacgcc gagcgaatta cggtacgtcg caaggacggc agctttatct gtgaggcgat
    29401 atggaacggc aataaacgcg cagcctttgc tgtcagtgct gaatatcaca aacaacagca
    29461 gcgtattaaa ggaatgcgca aacgcgctga ggaaaaaatt cgtgatgctg aagatgaagg
    29521 tattcagatt ctggaacaca aacaggctga accctggctg agtaatgttt atcgcccggt
    29581 tggtaatgtg gtggcagttc agcaaccgga atatgaagaa gagcatgacg aagaatttga
    29641 acgcgatttc cggctgggga tgcaaaaact ttttgccatg caggaagaag atgatccgct
    29701 ggcctgaata aaaaaacagt ccgcgggaac ggactgttaa ataaaggaaa caaagctgat
    29761 ttgaaataac cgaggaaata ctatgactga tattgatgat gt
//
LOCUS       DAEEGA010000048        29236 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEEGA010000048 DAEEGA010000000
VERSION     DAEEGA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29236
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.048"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..218
                     /locus_tag="KLH46_002285"
     CDS             <1..218
                     /locus_tag="KLH46_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0378945.1"
                     /translation="DTAMNFIRQGLGIALQPELTLKSIAGELCSVPHEPTFYRQISLL
                     TKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="KLH46_002286"
     CDS             complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="KLH46_002286"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBE0378946.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="KLH46_002287"
     CDS             complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="KLH46_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBE0378947.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2972..4600
                     /gene="glpA"
                     /locus_tag="KLH46_002288"
     CDS             2972..4600
                     /gene="glpA"
                     /locus_tag="KLH46_002288"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBE0378948.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4590..5849
                     /gene="glpB"
                     /locus_tag="KLH46_002289"
     CDS             4590..5849
                     /gene="glpB"
                     /locus_tag="KLH46_002289"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBE0378949.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5846..7036
                     /gene="glpC"
                     /locus_tag="KLH46_002290"
     CDS             5846..7036
                     /gene="glpC"
                     /locus_tag="KLH46_002290"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBE0378950.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7229..8179
                     /locus_tag="KLH46_002291"
     CDS             7229..8179
                     /locus_tag="KLH46_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBE0378951.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8220..8690)
                     /locus_tag="KLH46_002292"
     CDS             complement(8220..8690)
                     /locus_tag="KLH46_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBE0378952.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8745..9947)
                     /locus_tag="KLH46_002293"
     CDS             complement(8745..9947)
                     /locus_tag="KLH46_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBE0378953.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(10034..10576)
                     /locus_tag="KLH46_002294"
     CDS             complement(10034..10576)
                     /locus_tag="KLH46_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378954.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            10855..11280
                     /gene="nudI"
                     /locus_tag="KLH46_002295"
     CDS             10855..11280
                     /gene="nudI"
                     /locus_tag="KLH46_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBE0378955.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(11319..11921)
                     /locus_tag="KLH46_002296"
     CDS             complement(11319..11921)
                     /locus_tag="KLH46_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBE0378956.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            12229..13368
                     /gene="arnB"
                     /locus_tag="KLH46_002297"
     CDS             12229..13368
                     /gene="arnB"
                     /locus_tag="KLH46_002297"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBE0378957.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAESGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRTAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            13372..14340
                     /gene="arnC"
                     /locus_tag="KLH46_002298"
     CDS             13372..14340
                     /gene="arnC"
                     /locus_tag="KLH46_002298"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBE0378958.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            14340..16322
                     /gene="arnA"
                     /locus_tag="KLH46_002299"
     CDS             14340..16322
                     /gene="arnA"
                     /locus_tag="KLH46_002299"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HBE0378959.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            16319..17209
                     /locus_tag="KLH46_002300"
     CDS             16319..17209
                     /locus_tag="KLH46_002300"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBE0378960.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            17209..18861
                     /gene="arnT"
                     /locus_tag="KLH46_002301"
     CDS             17209..18861
                     /gene="arnT"
                     /locus_tag="KLH46_002301"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBE0378961.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            18858..19193
                     /gene="arnE"
                     /locus_tag="KLH46_002302"
     CDS             18858..19193
                     /gene="arnE"
                     /locus_tag="KLH46_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBE0378962.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVSQNVQVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            19193..19579
                     /gene="arnF"
                     /locus_tag="KLH46_002303"
     CDS             19193..19579
                     /gene="arnF"
                     /locus_tag="KLH46_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBE0378963.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="KLH46_002304"
     CDS             complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="KLH46_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBE0378964.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(19950..21305)
                     /gene="menE"
                     /locus_tag="KLH46_002305"
     CDS             complement(19950..21305)
                     /gene="menE"
                     /locus_tag="KLH46_002305"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBE0378965.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(21302..22264)
                     /gene="menC"
                     /locus_tag="KLH46_002306"
     CDS             complement(21302..22264)
                     /gene="menC"
                     /locus_tag="KLH46_002306"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBE0378966.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(22264..23121)
                     /gene="menB"
                     /locus_tag="KLH46_002307"
     CDS             complement(22264..23121)
                     /gene="menB"
                     /locus_tag="KLH46_002307"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBE0378967.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(23136..23894)
                     /gene="menH"
                     /locus_tag="KLH46_002308"
     CDS             complement(23136..23894)
                     /gene="menH"
                     /locus_tag="KLH46_002308"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBE0378968.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(23891..25561)
                     /gene="menD"
                     /locus_tag="KLH46_002309"
     CDS             complement(23891..25561)
                     /gene="menD"
                     /locus_tag="KLH46_002309"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBE0378969.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(25650..26945)
                     /gene="menF"
                     /locus_tag="KLH46_002310"
     CDS             complement(25650..26945)
                     /gene="menF"
                     /locus_tag="KLH46_002310"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBE0378970.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASLVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="KLH46_002311"
     CDS             complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="KLH46_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBE0378971.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(27384..27845)
                     /locus_tag="KLH46_002312"
     CDS             complement(27384..27845)
                     /locus_tag="KLH46_002312"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0378972.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            27910..28827
                     /gene="rbn"
                     /locus_tag="KLH46_002313"
     CDS             27910..28827
                     /gene="rbn"
                     /locus_tag="KLH46_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBE0378973.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKVFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            29018..29206
                     /locus_tag="KLH46_002314"
                     /pseudo
     CDS             29018..29206
                     /locus_tag="KLH46_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         7087 a         7856 c         7300 g         6993 t
ORIGIN      
        1 cggatacagc gatgaatttt attcgacagg gattaggcat cgccttacaa ccagagttaa
       61 cgctgaaaag cattgcaggt gaattgtgtt ccgttcctca cgaaccaacc ttctatcgac
      121 agatttcgtt gctgactaaa gaaaagccgg tagaaggcag tccactgttt ttactacaaa
      181 tgtgcatgga acaattagtg gcgattggaa aaatttgata tgagacagga tggcgcatga
      241 acgccatcct gcagtaacat tactctttat taagaaattt tactgcctta tcagggaaat
      301 cagtaaacag cccatttaca cccgctttgt tatacagagc atcatataac tgattcacat
      361 caggagtgta ttcaggcagt ttatctgacc gcacggtata aggatgcact accagtttat
      421 tctgctgggc atcttgcacc atgccagtga gtttgatatt acccggctgc gatgtctcct
      481 caatcaacat atggtaatcc ggaccaatac catctgcata ttccgccacc tgtttcatgg
      541 ctcccggctt aaacatccag tcgtagttgt aattaaccca gcttccatcc ggctgtttct
      601 gctgcgtttc attccagtcg gtataggcaa tcagctgtac cagattgagc tccatgccca
      661 ttttgggttc cagctcattc ttaatacgct tcagctcatc agcatcaaaa cattgcaaat
      721 aaactttatc gtctttaccg gtgtaaccat atttcttcag cacttccagc gtttttgccg
      781 caatatcctt cccttcctga tgatggaacc acggcgcttt gatttctgga tagataccga
      841 tatttttccc ggtagagtga tttaacccct gaacaaattc aatctcttct tcaaaggtgt
      901 gcacccggaa gtcggactta cccattggga aacgccccgg ataagtctgt acttttttac
      961 cgttttcaat atcgaaacct tcggtaaatt tcagcgactt aatttcatcc agcgtgaaat
     1021 ctatcgcgta gtaacgaccg tctttgcgcg cccgatccgg gaaacgatcg gcaacatcag
     1081 taacacgatc gagataatgg tcatgcagaa caaccagatg gtcgtctttg gtcatcacca
     1141 aatcctgttc cagataatcc gctccctgcg catacgccat cgcttttgct ggcagcgtat
     1201 gctccggcaa atatccactg gcaccgcgat gggcgatgac tattttttcg ttgctgtccg
     1261 ccgccattgc actgcttccc atgactatag tgctcatcat gatcgccatg ctaaggtttt
     1321 tcagcttcaa tttcatgcca ttagcctccg ttgcgttttt gcagtaattg ttcatggcga
     1381 cgtttttcgc caatcatcac aacaatcaac aagataaccg ccagaatgct gccgccaatc
     1441 attaccataa agccgccatc ccagccgaag aagtccacgg tgtagccaac aatcgcgctc
     1501 gccgccaccg aaccgcccag gtaaccaaac agcccggtaa agcccgctgc cgtacctgcc
     1561 gcttttttcg gtgccagttc cagcgcatgc agaccgatca gcatcacagg accgtagatc
     1621 aggaagccga taacaatcat acaaatcata tcgacggttg ggttacctgc cgggttcatc
     1681 cagtaaacga tagtcgcgat ggtcaccagt gtcataaaga agacgccggt tgccccacgg
     1741 ttaccacgga agactttgtc cgacatccag ccgcacagca gggtgcccgg aatacccgca
     1801 tactcataga agaagtaggc ccaggaggat ttatccagcg cgaaatgctt aacctctttc
     1861 agataagtcg gtgaccagtc gaggatgccg taacgcagca gataaacgaa cacgttggcg
     1921 atggcgatgt accacagcag tttgttcggc agcacgtact gcatgaagat ttgcttcgcg
     1981 gtcagctcct gttccgcttt ttcgttatag tcgtccggat aatcattttt gtactcttcg
     2041 atcggcggca agccacagga ttgcggggta tcgcgcatca tcgcaaaggc gaataatgcc
     2101 accagaatgg cgcagaaagc aggcatatag agcgccgcat gccagtcatt gaaccaggcc
     2161 atccccagca ggaacagcag cggcggaata ccaccaccga cgttgtgcgc acagttccac
     2221 actgacacaa tgccgccacg ttctttctgc gaccaccagt gcaccatagt acgaccacac
     2281 ggcggccacc ccatcccctg gaaccaaccg cagaggaaca acagtacaaa catcaccgca
     2341 atgctcgacg tcgcccatgg cacaaagccc ataaacaaca tcactgccgc cgccagaatc
     2401 aaacctgcgg gcaggaaaac gcgcggattc gagcgatccg ataccgaacc catgatgaat
     2461 ttcgaaaatc cataagcaat cgagatcccc gaaagggcaa aacctaaatc accgcgtgag
     2521 aatccctgct caaccagata aggcatagca agcgcaaagt tcttacgaac caaatagtaa
     2581 gccgcatagc caaagaatat ccccaggaaa atttgccagc gcaatcgacg ataagtcgga
     2641 tcgatctccg cggcaggtaa gcgcgctttg tgtggcgctg gtttaaaaat actcaacatt
     2701 gatagcctcc gtggcccgtg gtcttattta tgattaacag cctgattcag tgagagaacc
     2761 tgccgtttct tgagttgccg cgatgttaag aaaacattca taaattaaat gtgaattgcc
     2821 gcacacatta ttaaataaga tttacaaaat gttcaaaatg acgcatgaaa tcacgtttta
     2881 ctttcgaatc atgagcgaat atgcgcgaaa tcaaacaatt catgttttta ctatggctaa
     2941 atggtaaaaa acgaacttca gagggataac aatgaaaact cgcgactcgc aatcaagtga
     3001 cgtgattatc attggcggcg gcgcaacggg agccgggatt gcccgcgact gtgccctgcg
     3061 cgggctgcgc gtgattttgg ttgagcgcca cgacatcgca accggtgcca ccgggcgtaa
     3121 ccacggcctg ctgcacagcg gtgcgcgcta tgcagtaacc gatgcggaat cggcccgcga
     3181 atgcattagt gaaaaccaga tcctgaaacg cattgcacgt cactgcgttg aaccaaccaa
     3241 cggcctgttt atcaccctgc cggaagatga cctctcattc caggccactt ttattcgcgc
     3301 ctgcgaagaa gcagggatca gcgcagaagc tatagacccg cagcaagcgc gcattatcga
     3361 acctgccgtt aacccggcac tgattggcgc ggtgaaagtt ccggatggca ccgttgatcc
     3421 atttcgtctg accgcagcaa acatgctgga tgccaaagaa cacggtgccg ttatccttac
     3481 cgctcatgaa gtcacggggc tgattcgtga aggcgcgacg gtgtgcggtg ttcgtgtacg
     3541 taaccatctc accggcgaaa ctcaggccct tcatgcacct gtcgtggtta atgccgctgg
     3601 gatctggggg caacacattg ccgaatatgc cgatctgcgc attcgcatgt tcccggcgaa
     3661 aggatcgctg ctgatcatgg atcaccgcat taaccagcat gtgatcaacc gctgccgtaa
     3721 accttccgac gccgatattc tggtgcctgg cgataccatt tcgctgattg gtaccacctc
     3781 tttacgtatt gattacaacg agattgacga taatcgagtg acggcagaag aggttgatat
     3841 tctgctgcgt gaaggggaaa aactggcccc cgtgatggcg aaaacgcgca ttttgcgggc
     3901 ctattctggc gtgcgcccgc tggttgccag cgatgacgac ccgagcggac gtaacgtcag
     3961 ccgtggcatc gtgctgctcg accatgctga acgcgatggt ctggacggat ttatcaccat
     4021 caccggtggc aaactgatga cctatcggct gatggctgaa tgggctaccg acgcggtatg
     4081 ccgcaaactg ggcaacacgc gcccctgtac gactgccgat ctggcactgc ctggttcaca
     4141 agaacccgct gaagttacct tgcgtaaagt catctccctg cctgccccgc tgcgcggttc
     4201 tgcggtttat cgtcatggcg atcgcacgcc tgcctggctg agcgaaggcc gtctacaccg
     4261 tagcctggta tgtgagtgcg aagcggtaac tgcgggtgaa gtgcagtacg cggtagaaaa
     4321 tttaaacgtt aatagcctgc tggatttacg ccgtcgtacc cgtgtgggga tgggcacctg
     4381 ccagggcgaa ctctgcgctt gccgcgctgc cggactgctg caacggttta acgtcacgac
     4441 gtccgcgcaa tctatcgagc aactttccac cttccttaac gaacgctgga aaggcgtgca
     4501 acccatcgcc tggggagacg cactgcgcga aagcgaattt acccgctggg tttatcaggg
     4561 actgtgtggt ctggagaagg agcagaaaga tgcgctttga tactgtcatt atgggcggtg
     4621 gcctcgccgg attactctgt ggcctgcaac tgcaaaaaca cggcctgcgc tgtgccattg
     4681 tcacgcgtgg tcaaagcgcg ttgcatttct catccggttc gctggatttg ctgagccatc
     4741 tgccagatgg tcaacccgtg gcagacattc acagtggact ggaatctttg cgtcagcagg
     4801 cacctgcaca tccttactct cttctcgggc cacaacgcgt gctcgatctc gcttgccagg
     4861 cgcaggcatt aatcgctgaa agcggtgcgc aattgcaggg cagcgtagaa ctggctcacc
     4921 agcggattac gccgctcggc actttgcgtg caacctggtt aagttcgcca gaagttcccg
     4981 tctggccgct acccgcgaag aagatatgtg tagtggggat aagcgggctg atggatttcc
     5041 aggcgcatct tgcggcggct tcgttgcgcg aactggatct ttcggttgaa accgcagaaa
     5101 tagagctgcc ggaactggat gtcctgcgca ataacgccac cgaatttcgc gcggtgaata
     5161 tcgcccgttt ccttgataat gaagaaaact ggccgctgtt acttgatgcg cttattcctg
     5221 tcgccaatac ctgcgaaatg atcctgatgc ccgcctgctt cggcctggcc gatgacaaac
     5281 tgtggcgttg gttgaatgaa aaactacctt gttcactgat gcttttgcca acgctgccgc
     5341 cttccgtgct gggcattcgt ctgcaaaacc agttacagcg ccagtttgtg cgccaaggtg
     5401 gagtgtggat gccgggcgat gaagtgaaaa aagtgacctg taaaaatggc gtagtgaatg
     5461 aaatctggac ccgcaatcac gccgatattc cgctacgtcc acgtttcgcg gttctcgcca
     5521 gcggcagttt ctttagtggc ggactggtag cggaacgtaa cggcattcgc gagccgattc
     5581 tcggccttga tgtgctacaa accgccacgc ggggtgaatg gtataagggc gatttttttg
     5641 cgccgcaacc gtggcagcag ttcggtgtaa ccactgatga gacgctacgc ccgtcgcagg
     5701 cagggcaaac cattgagaac ctgtttgcca tcggttcggt gctgggcgga tttgatccca
     5761 tcgcccaggg atgcggcggc ggtgtttgtg ccgtcagtgc tttacatgcc gctcaacaga
     5821 ttgcccaacg cgcaggaggc caacaatgaa cgacaccagc ttcgaaaact gcattaagtg
     5881 caccgtctgc accaccgcct gcccggtgag ccgggtgaat cccggttatc cagggccaaa
     5941 acaagccggg ccggatggcg agcgtctgcg tttgaaagat ggcgcactgt atgacgaggc
     6001 gctgaaatat tgcatcaact gcaaacgttg tgaagtcgcc tgcccgtccg atgtgaagat
     6061 tggcgatatt atccagcgcg cgcgggcgaa atatgacacc acgcgcccgt cgctgcgtaa
     6121 ttttgtgttg agtcataccg acctgatggg tagcgtttcc acgccgttcg caccaatcgt
     6181 caacaccgct acctcgctga aaccggtgcg gcagctgctt gatgcggcgt taaaaatcga
     6241 tcatcgtcgc acgctaccga aatactcctt cggcacgttc cgtcgctggt atcgcagcat
     6301 agcggctcag caagcacaat ataaagacca ggtcgctttc tttcacggct gcttcgttaa
     6361 ctacaaccat ccgcagttag gtaaagattt aattaaagtg ctcaacgcaa tgggtaccgg
     6421 tgtacaactg ctcagcaaag aaaaatgctg cggcgtaccg ctaatcgcca acggctttac
     6481 cgataaagca cgcaaacagg caattacgaa tgtagagtcg atccgcgaag ctgtgggagt
     6541 aaaaggcatt ccggtgattg ccacctcctc aacctgtaca tttgccctgc gcgacgaata
     6601 cccggaagtg ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac tggcaacccg
     6661 ctggctgtgg cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa
     6721 agtggtttat cacactccgt gccatatgga aaaaatgggc tggacgctct acaccctgga
     6781 gctgttgcgt aacatcccgg ggcttgagtt aacggtgctg gattcccagt gctgcggtat
     6841 tgcgggtact tacggtttca aaaaagagaa ctaccccacc tcacaagcca tcggcgcacc
     6901 actgttccgc cagatagaag aaagcggcgc agatctggtg gtcaccgact gcgaaacctg
     6961 taaatggcag attgagatgt ccacaagtct tcgctgcgaa catccgatta cgctactggc
     7021 ccaggcgctg gcttaaactc ccttctgatg cccggtaagc atgtggttac cgggcatttt
     7081 tgcgtacacg attccgtgcc caatgtatgc gttgcaacgc agtgaaaatt cctctgaaaa
     7141 cgtctcgcaa aggctgaaac tggcagatgt caaaggcctg ggataaccgt aatgtcgcgt
     7201 catcataaat atcaggtgac ggacaaccat gaccgaatca acaacctcct ccccgcatga
     7261 tgcggtattt aaaaccttta tattcacacc cgaaaccgca cgggattttc tcgaaataca
     7321 tttaccagaa ccactgcgca agctttgcaa cctgcaaacc ttacgcctgg aacccactag
     7381 ttttattgaa aaaagtttac gcgcttacta ctcggatgtt ttgtggtccg tggaaaccag
     7441 cgacggtgac ggctatatct actgcgtgat tgaacatcaa agctctgcag aaaagaatat
     7501 ggcttttcgg ccaatgcgct atgccactgc cgccatgcag agtcacctag ataaaggcta
     7561 tgacagagtt ccgctggtgg tgccattgct gttttatcat ggcgaaacat cgccctaccc
     7621 gtactcactt aactggctgg atgagtttga cgatccgcaa cttgcccggc agttgtacac
     7681 cgaagctttt ccgttggtgg atatcaccat cgtacctgac gatgagatca tgcaacatcg
     7741 gcgtatagct ctgctggaac tgattcaaaa gcatattcgc gaccacgatt taatcggcat
     7801 ggtcgacagg atcaccacgc ttttggttag aggcttcact aatgacagcc agctacaaac
     7861 actgtttaat tatctgctgc aatgcggcga tacctcccgt ttcacccgtt ttattcagga
     7921 gattgccgaa cgttcaccac tacaaaagga gagattaatg actattgctg aacggctacg
     7981 gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc agcaaggtaa
     8041 attagaaggt ttgcaggaag gtatgcatga acaagccatt aaaattgctt tgcgcatgct
     8101 ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg aagccgatct
     8161 ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg cctttgatat
     8221 caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc cagtcgttga
     8281 tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt cgctccccac
     8341 gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc tttacccgca
     8401 gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg cccggcgtta
     8461 tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc atcaatccct
     8521 tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac ctgcactaac
     8581 agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca gcgcgcagcc
     8641 cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa attttgccgg
     8701 agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac ggataaagtc
     8761 atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc gtttaaccaa
     8821 cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc caggctgtag
     8881 cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc tagcgcaaag
     8941 ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggcc
     9001 cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaatcact
     9061 tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa accaccggtg
     9121 aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg
     9181 ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc
     9241 cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc
     9301 ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt
     9361 tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcggctgt
     9421 ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat caccttaaat
     9481 tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac ggcaaaacca
     9541 caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta
     9601 cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc
     9661 caggcttcat gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcgcttaaa
     9721 tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt
     9781 tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat
     9841 ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc
     9901 ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt tatctcctgc
     9961 ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatgcc
    10021 gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca gccacagcgt
    10081 tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc tcaatactga
    10141 ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt tgaataccgc
    10201 tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg ttgccaattt
    10261 tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa ttcgggttgg
    10321 tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga ttcaacccca
    10381 gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg cttaaagcca
    10441 ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta cccgcctggc
    10501 cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc agacctgcta
    10561 gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc ccaaagctgc
    10621 gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct agtcacttat
    10681 tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact ttaccgatac
    10741 acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc actttcacat
    10801 atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac ccctgtgcga
    10861 caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct gtgcaaaatg
    10921 gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct
    10981 ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg agaacagctg
    11041 cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa gacatatgca
    11101 gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt ttctgccaac
    11161 cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc tgaagatctg
    11221 gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg tcttctttaa
    11281 caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac gaattcccca
    11341 tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata atcaggttta
    11401 aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg ggtgaaaata
    11461 acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata aatctcatta
    11521 ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa ccaggtcgcc
    11581 gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat gtcagcgcta
    11641 aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc tgttttatct
    11701 gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc
    11761 gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa accattgcta
    11821 ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa aatgatgaaa
    11881 tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc acctatagac
    11941 aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa ttagcagttg
    12001 gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc caaatgcaaa
    12061 gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag tttcttaagg
    12121 ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac acaacactct
    12181 ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt
    12241 ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc
    12301 gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc cttttgccag
    12361 ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg
    12421 ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt
    12481 tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga tgtcgaccgc
    12541 gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg cactaaagcc
    12601 atcattccgg tgcattatgc cggtgcgcca gcagatattg acgccattcg cgccattggc
    12661 gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac gtattacaaa
    12721 gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt
    12781 acctgtgctg aaagtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg
    12841 atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca
    12901 ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat taacgccgcg
    12961 attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg cgaaattgcc
    13021 cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgcctgg
    13081 ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc
    13141 agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg gttacatttc
    13201 cgcaccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat
    13261 accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc
    13321 gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa
    13381 atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta
    13441 ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga gtatgagatc
    13501 ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa
    13561 gcggagaaca gccatattgt gtctattttg attaaccgca attacgggca acattcagcg
    13621 attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga tgccgatctc
    13681 cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg
    13741 gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc ttcgaagatg
    13801 attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg ttgtatgctg
    13861 cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt
    13921 atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt acatcatgcc
    13981 gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt gatgtacgac
    14041 ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg cagcattatt
    14101 gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca
    14161 caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct
    14221 cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc
    14281 gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa
    14341 tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa gctctgctgg
    14401 ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt gaaaaagcct
    14461 tttatggttc ggtggctcat ctagcggcgg aaagagacat tccggtttat gcgccggata
    14521 acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag gtgattttct
    14581 ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc gcaggtgcat
    14641 ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc
    14701 tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa cgtgccgatg
    14761 ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc gctattacgc
    14821 tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta cccgccatta
    14881 aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca
    14941 gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg cacaacatgg
    15001 tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat cagaaattca
    15061 ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt
    15121 ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg
    15181 cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc ctggtgcaag
    15241 gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc
    15301 tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt
    15361 atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccac
    15421 attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag tatcacgtca
    15481 aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca
    15541 acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg
    15601 ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg
    15661 ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct
    15721 ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat ggcgaaaaag
    15781 agggtttaca gttcaccctc ttcctcccgt ttaactggat gggaccacga ctggataacc
    15841 ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc aatctggtag
    15901 aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt actgatatcc
    15961 gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg
    16021 aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc
    16081 tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt gcgggctttc
    16141 gcgttgtcga aagtagctgc tactacggca aaggatatca ggacgtagag catcgtaaac
    16201 cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat atgcaggaaa
    16261 ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat
    16321 gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc
    16381 gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg
    16441 cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat
    16501 gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag gtacggcctg
    16561 gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca
    16621 cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg gtaactggga
    16681 tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag agattatcgg
    16741 tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc
    16801 aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatac cgttccgtcc
    16861 actgcttgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac ccacctggga
    16921 tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac tcaaccgcat
    16981 cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta
    17041 tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc
    17101 tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg gaaatattgc
    17161 cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta
    17221 cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg
    17281 cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca
    17341 tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa acccattgcc
    17401 ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg
    17461 gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt tactctgcgc
    17521 ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc attgtttatt
    17581 gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg
    17641 ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga
    17701 tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt
    17761 gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc
    17821 tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc ctggggactg
    17881 gcgatagcgc agcgggagcc tgacttctgg cactattttt tctgggttga gcatattcaa
    17941 cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc
    18001 attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa
    18061 aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt
    18121 ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc acctctggca
    18181 atgctgttgg cgcattacgc gttgctggca gcaaaaaata atcctctggc gctgcggatt
    18241 aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtgatctcc
    18301 ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa agtcttttgt
    18361 gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa
    18421 aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca
    18481 attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa
    18541 tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggca
    18601 tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc
    18661 cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt
    18721 aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat
    18781 atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg tctggtgctg
    18841 attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg
    18901 ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac
    18961 atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg atgctctggc
    19021 tgctggtctc gcagaacgta caggtaggca ttgcctaccc gatgttaagt ctgaattttg
    19081 tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt
    19141 gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgggcct
    19201 gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc
    19261 ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt
    19321 tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg
    19381 ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag
    19441 ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct
    19501 gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccac
    19561 gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc actcatcggg
    19621 gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata
    19681 aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac
    19741 ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc
    19801 ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc tgtttgctaa
    19861 gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg
    19921 cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc
    19981 gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt cagccagcgc
    20041 accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc aacggtttgc
    20101 tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc aacggaaacg
    20161 ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc ttccggctga
    20221 ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt cagcttgcca
    20281 ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc
    20341 agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta
    20401 ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag gccgtcggct
    20461 tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc
    20521 ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc
    20581 gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt
    20641 gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc
    20701 cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt
    20761 aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac accttgcgca
    20821 ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt agaacccgag
    20881 gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac gccttcaacc
    20941 agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca
    21001 aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc
    21061 acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag cgtttgcggc
    21121 gtattccacg cccgcaacat cacgccgctg ccctcaacca ccccctgcgc cgcaaagccc
    21181 gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga
    21241 cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag
    21301 atcataacaa ctgctcctgt acatcagcgt ccactaacgg cagtgggctc cccggccagc
    21361 gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg
    21421 ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca atggaagaac
    21481 tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt actttttcca
    21541 gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg
    21601 caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt tcacgggcaa
    21661 acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat
    21721 aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg
    21781 cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt
    21841 cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc ggcatatcgg
    21901 caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca cggtagttgg
    21961 ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca aaggccacgg
    22021 aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag gcaagcagca
    22081 cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag atctcccccc
    22141 acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc
    22201 tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac
    22261 gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcgtt
    22321 gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc
    22381 ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc
    22441 catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt ccagatccgc
    22501 cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg
    22561 gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca tgtaggaagc
    22621 gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc
    22681 tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggagtagcc
    22741 agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac
    22801 gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg
    22861 gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgatatt
    22921 gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa
    22981 ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc
    23041 ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaggcat
    23101 tgcttcatca ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga
    23161 cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca
    23221 tggcagtcgg cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat
    23281 aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg
    23341 agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc
    23401 accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg
    23461 gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg atcggaacgc
    23521 tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc gacaataacc
    23581 ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag
    23641 taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg cagtaagtcg
    23701 gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc taggagatca
    23761 acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc gtggcaatcg
    23821 ccggaaaaac cgtgcaaaaa caccagccag ggtaaaccta gttttccgtg ttttgcctgc
    23881 gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat
    23941 cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag gcgtcggcaa
    24001 atgccgtttc aagttcctgc cagttttgcg gatgatgata tttcagctcg aacatcgcgg
    24061 cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc tcgctttgcg
    24121 gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg
    24181 gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagcgcg gagagatcgc
    24241 ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg gtcgaaagca
    24301 gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc ggaagttgcg
    24361 aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc ccctgttcag
    24421 gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca cggcgggcaa
    24481 taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt
    24541 tttctgccgg atgcagctcc agccagtcgg caatattggc aattaagcga cgtccgcgat
    24601 ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac
    24661 agcttgcctg ccattgcagg agccgtttcc ccgtcaggct acttcccagt tgcaccacaa
    24721 tttgcgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga tcggcacacg
    24781 gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca agagtttgcg
    24841 cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc
    24901 gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga ggcgcttcac
    24961 gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag ctaagcccgg
    25021 tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg actccccccg
    25081 catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct
    25141 gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt cccggctggc
    25201 gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaagagaa
    25261 tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt gccaccgccg
    25321 tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca
    25381 gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg
    25441 ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt ctgacgccgt
    25501 gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca
    25561 tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg
    25621 atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct
    25681 gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc
    25741 gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag ggaaacgcag
    25801 aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac
    25861 ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc
    25921 gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata
    25981 cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc
    26041 gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc
    26101 tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga
    26161 tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat
    26221 aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg
    26281 tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt gaccagactt
    26341 gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc ttcggcgatg
    26401 gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct
    26461 cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat
    26521 tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc
    26581 gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt gccctgcgac
    26641 gggtcaaaag cattcagccc ccaaatgcgt aagtcagcgt gttccgggtg ctggcgaaga
    26701 aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct
    26761 tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag
    26821 ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt
    26881 gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat
    26941 tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg
    27001 cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagcccg
    27061 acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga
    27121 taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc
    27181 acatcatcca gcgctttttc tgcatgcgct ttcagctcaa catatttctg atcggcggga
    27241 tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg
    27301 atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac
    27361 taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat cccaatgtgt
    27421 gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact ctggtagaag
    27481 ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg atgcgtacat
    27541 gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa cgcttcactg
    27601 acaatcaccc gacctataac gaccggctca agatcatcat cacttttcag aatcctcgca
    27661 tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca
    27721 tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc gcaacgcagt
    27781 tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca
    27841 atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt
    27901 gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac ccgcacgcgc
    27961 aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact ttggttgttt
    28021 gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg
    28081 gacaaggttt ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg
    28141 tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc
    28201 cgtgaatttg tggaaaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa
    28261 attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt aaccgcttat
    28321 ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga taaaccgggt
    28381 gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact gtttcaggaa
    28441 ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg cgcagattac
    28501 ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat
    28561 gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata
    28621 accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc tgcgacacta
    28681 gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg ctatgatgac
    28741 aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactggcg
    28801 aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gtgaaacttg
    28861 ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg agcgattttt
    28921 gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc ttttcatctt
    28981 gattcagcac aacctttgca actaaaagga gcccatgatg gttacagttg tcagcaatta
    29041 ttgtcaatta tctcaaaccc aactcagtca gacatttgca gaaaaattta ctgtgaccga
    29101 ggaattactg cagtctttaa aaaaaacagc gttatccgga gatgaagaaa gtattgagtt
    29161 actgcataat attgcgttag gttatgatga atttggaaaa aaagctgggt aatgctgcca
    29221 acttactgat ttagtg
//
LOCUS       DAEEGA010000049        29197 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEEGA010000049 DAEEGA010000000
VERSION     DAEEGA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29197
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.049"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(177..1073)
                     /gene="yhaJ"
                     /locus_tag="KLH46_002315"
     CDS             complement(177..1073)
                     /gene="yhaJ"
                     /locus_tag="KLH46_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HBE0378974.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            1178..1879
                     /locus_tag="KLH46_002316"
     CDS             1178..1879
                     /locus_tag="KLH46_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBE0378975.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1902..2066
                     /locus_tag="KLH46_002317"
     CDS             1902..2066
                     /locus_tag="KLH46_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378976.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2278..3588)
                     /locus_tag="KLH46_002318"
     CDS             complement(2278..3588)
                     /locus_tag="KLH46_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBE0378977.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3616..4947)
                     /locus_tag="KLH46_002319"
     CDS             complement(3616..4947)
                     /locus_tag="KLH46_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBE0378978.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(5222..6586)
                     /gene="tdcG"
                     /locus_tag="KLH46_002320"
     CDS             complement(5222..6586)
                     /gene="tdcG"
                     /locus_tag="KLH46_002320"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBE0378979.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6658..7047)
                     /locus_tag="KLH46_002321"
     CDS             complement(6658..7047)
                     /locus_tag="KLH46_002321"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBE0378980.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7061..9355)
                     /gene="tdcE"
                     /locus_tag="KLH46_002322"
     CDS             complement(7061..9355)
                     /gene="tdcE"
                     /locus_tag="KLH46_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBE0378981.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9389..10597)
                     /gene="tdcD"
                     /locus_tag="KLH46_002323"
     CDS             complement(9389..10597)
                     /gene="tdcD"
                     /locus_tag="KLH46_002323"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBE0378982.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10623..11954)
                     /gene="tdcC"
                     /locus_tag="KLH46_002324"
     CDS             complement(10623..11954)
                     /gene="tdcC"
                     /locus_tag="KLH46_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBE0378983.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11976..12965)
                     /gene="tdcB"
                     /locus_tag="KLH46_002325"
     CDS             complement(11976..12965)
                     /gene="tdcB"
                     /locus_tag="KLH46_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBE0378984.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13064..14002)
                     /gene="tdcA"
                     /locus_tag="KLH46_002326"
     CDS             complement(13064..14002)
                     /gene="tdcA"
                     /locus_tag="KLH46_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBE0378985.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14192..14536
                     /gene="tdcR"
                     /locus_tag="KLH46_002327"
     CDS             14192..14536
                     /gene="tdcR"
                     /locus_tag="KLH46_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBE0378986.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            14792..15331
                     /locus_tag="KLH46_002328"
     CDS             14792..15331
                     /locus_tag="KLH46_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378987.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            15353..15700
                     /locus_tag="KLH46_002329"
     CDS             15353..15700
                     /locus_tag="KLH46_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378988.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            15869..16519
                     /locus_tag="KLH46_002330"
     CDS             15869..16519
                     /locus_tag="KLH46_002330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0378989.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(17140..17516)
                     /gene="rnpB"
                     /locus_tag="KLH46_002331"
     ncRNA           complement(17140..17516)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KLH46_002331"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(17549..18694)
                     /gene="garK"
                     /locus_tag="KLH46_002332"
                     /pseudo
     CDS             complement(17549..18694)
                     /gene="garK"
                     /locus_tag="KLH46_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(18791..19681)
                     /gene="garR"
                     /locus_tag="KLH46_002333"
     CDS             complement(18791..19681)
                     /gene="garR"
                     /locus_tag="KLH46_002333"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBE0378990.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(19711..20481)
                     /gene="garL"
                     /locus_tag="KLH46_002334"
                     /pseudo
     CDS             complement(19711..20481)
                     /gene="garL"
                     /locus_tag="KLH46_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(20497..21831)
                     /gene="garP"
                     /locus_tag="KLH46_002335"
     CDS             complement(20497..21831)
                     /gene="garP"
                     /locus_tag="KLH46_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBE0378991.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGLKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            22206..23777
                     /gene="garD"
                     /locus_tag="KLH46_002336"
     CDS             22206..23777
                     /gene="garD"
                     /locus_tag="KLH46_002336"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBE0378992.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVEIASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(23829..24653)
                     /gene="agaR"
                     /locus_tag="KLH46_002337"
     CDS             complement(23829..24653)
                     /gene="agaR"
                     /locus_tag="KLH46_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBE0378993.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            24902..26182
                     /gene="kbaZ"
                     /locus_tag="KLH46_002338"
     CDS             24902..26182
                     /gene="kbaZ"
                     /locus_tag="KLH46_002338"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HBE0378994.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            26205..26678
                     /gene="agaV"
                     /locus_tag="KLH46_002339"
     CDS             26205..26678
                     /gene="agaV"
                     /locus_tag="KLH46_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBE0378995.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            26689..27468
                     /locus_tag="KLH46_002340"
     CDS             26689..27468
                     /locus_tag="KLH46_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBE0378996.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            27458..28267
                     /locus_tag="KLH46_002341"
     CDS             27458..28267
                     /locus_tag="KLH46_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBE0378997.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            28354..28788
                     /locus_tag="KLH46_002342"
     CDS             28354..28788
                     /locus_tag="KLH46_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE0378998.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            28785..>29197
                     /locus_tag="KLH46_002343"
     CDS             28785..>29197
                     /locus_tag="KLH46_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBE0378999.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         7594 a         7622 c         6890 g         7091 t
ORIGIN      
        1 attgttgaca aagtgcgctt tgtttatgcc ggatgcggcg taaacgcctt atccagccta
       61 caaattgcca aaattcaata tattgcagtg actgcgtagg cctgataagc gtagcgcatc
      121 aggcagtttt gcgtttgtca gcagtcagaa gcaccttgtg aaaggtgctt aatagattat
      181 tttccagcga acaactttgg aatttcccgc agacaccagg atttcgcttc ccccatactg
      241 tcacgacgcc aggccataat aatatcgatc tcgctggtcg attccgggct gacgacacgc
      301 aaccgccctt ccgcaatatc tttttcgacc atcggatacg gcatcgtcgc cacgccaagc
      361 cccgccagta atgcctgacg tttatcttca atcgtgctca ccgttaagcg cggctgtttg
      421 tccagcagct gtacggtcaa caccgggcgc tcacgagcgg tatccgctac cgcaattcca
      481 cgatatttca cacgcgtcac ttcagataac ggttccggct cctgatgaat cgggtgatca
      541 ggcgcggcaa cgtagacgtt cattaacgta tagagcttgc gcgagttgat ctccgacgag
      601 gaacgaaaat gcatatccgg cgcgataacg atatccgccc gcccctgctc cagccgttcc
      661 cacgcccccg ccagcacttc tgtgatgatt gccagttggg tattggcttt tgccgccagt
      721 ttgtcgatta acgggaaaaa ggcaggtgtc ggtaccagcg cttcggtcac aatggtgaga
      781 tgcgtttccc aaccgcgcgc gagagcttcc gcatcagtag tcagtttatc tgcggcttcc
      841 agcaaaacgc gccctcgctc cagcaacatc ctcccgacat tggtgaattt ggtgcgatgg
      901 cccgagcggt caaacagcac cacatccagc tcttcttcca gtttttgcat ggtgtagcta
      961 agtgcggaag gcacgcgtcc cagctcatcc gctgccgccg caaaactgcc ccggcgatcg
     1021 atcgcatcca taacccgtag tgcttccagc gttaatgccc tttctttggc catttcgttc
     1081 tcattcagga aatttgaaca taccgggcag aatatctggc taacaatgta gcgtccagcc
     1141 ccttaccata aaaggaagta aagagaggtc aagaattatg attactaccc gaactgccag
     1201 gcagtgtgga caagcagact acggatggtt gcaggcccgg tatacttttt cctttggaca
     1261 ctacttcgac ccgaaattgt taggctatgc ctccctgcgt gtgcttaacc aggaagtgct
     1321 ggccccaggt gccgcctttc agccgcgaac ctatcccaaa gtcgatattt taaatgtgat
     1381 tctggatgga gaagcagagt atcgcgacag cgaaggcaat catgttcagg ccagcgccgg
     1441 agaggcgttg ctgctctcta cccagccggg tgtcagctat agcgaacaca atctcagcaa
     1501 agacaaaccg ttaacgcgaa tgcagctttg gctggatacc tgcccgcagc gagagaatcc
     1561 gctgattcaa aagctggcgc ttaatatgga caagcagcaa ttaatcgcct cgccagaggg
     1621 tgcgatggga agcctgcaat tacgccaaca agtgtggctg caccatatcg tgctcgacaa
     1681 aggcgaaagt gcgaatttcc agttgcatgg gccacgcgcg tatttgcaat cgattcacgg
     1741 gaaatttcat gcgcttacgc atcatgaaga gaaagcggcg ctaacctgcg gtgatggggc
     1801 gtttattcgt gacgaggcta acattacgct ggttgccgat tccccactgc gcgctttgct
     1861 gatagatttg cctgtctagt tgtttttaca gggagatgat gatgagtaaa aaatcgacca
     1921 aaaagcgcca gccagcgaag cccgtggtgg cgaaagaacc tgctcgcacc gccaaaaatt
     1981 ttggctatga agagatgttg agcgagctgg aagctatcgt cgcggatgct gaaacgcgtt
     2041 tagccgagga tgaagctacc gcgtaaagac aggttgccgg atgcggcgta aacgccttat
     2101 ccggcctaca aaacacaaca acatcaacaa attaaacacc ccgtaggccg gataagatgc
     2161 gccagcatcg tatccggcat caggtacaaa acacaacaac atcaacaagt tgagcacccc
     2221 gtaggccgga taaggtgcgc cagcatcgca tccggcacaa tactcaaaac ctggcgttta
     2281 tctggccttg ctcgccataa tctcgataat ctgccgatcc gtttgctgca tcgaatggct
     2341 tgctaacgca cacaggttgg caatcgactg ctcaacatca tgcgccacga tcccttcatt
     2401 gccggtcacg gcggtatcat ccagcgccat taacaccgct ttccacgcag ccgaagcact
     2461 ggtcgaaacc ttcatcgcgc agctgttcga cgcaccatcg caaatcatgc cgctgacatc
     2521 gccgatcata ctgctgatcg ccatcgagat agtttcataa cgcccatcca ccagccatgc
     2581 catcccggcg gcggccccca ttgctgcggt cgttgcggca caaagtgcag acaaacgcgg
     2641 taactggtta tggatgtaaa ttgcgctcaa atgagaaagc atcagcgcac gcgccagtcg
     2701 ttcatcatcc gctccgaagt gttctgctac aaccaccaca ggcattattg cagtgatccc
     2761 ctggttaccc gagccggagt tactcatagc cggaagcgta gcgccgccca tacgcgcatc
     2821 ggatgccgcg ctggtacgaa tcacaatgga tgaagagaga tctttcgcca gcaaaccgcg
     2881 ctcgcactgt ttttccagcg tcgcgccaat atgcagcccc cacttaccgc tcaaaccttc
     2941 ctgcgataac gcacaattta gcttcgcgga atcgagaata aagcggatcg ccgcaaatgg
     3001 gacttcattg acgaacttca ggatctcagc cagcgtcgtt ctggaaagca ccgtcagcgg
     3061 cgactcttgc tcgccctctg ccacacacgc ctggtgggta aacaccacac cattgtgcgt
     3121 ctcaatatgc acaatgttgg tatgcccgcc gacgatggtg acacacgccc acttctcacc
     3181 gttccagact ttggcgcgtg agaagaggat ttcattgcaa ggttcctgga tcttaacaga
     3241 gactttcccc gccgccagca gtgctttggc atcggcaatt gcctgcgcag ttgcgtcttt
     3301 cagcacttcc agcccggcgt tggcatttcc acctaacgcc cccagcgccg ccgcaatcgg
     3361 cagccccacc attcccgtgc cgggaacggt gacgcccaga ccgttcttca tcagatttgg
     3421 cgaaacccag gcttctacac gttcaaccgg accttccagt tctgccgcag caaccgccgc
     3481 cgccagcgcc agtgaaatcg gttcagtaca tcccagcgcc ggttttactt cctcctgaac
     3541 ggcgaggatg taacgctgcc ataacggatt taaagtcgaa tcaaacatat taaaaacctt
     3601 aaaatttcag gtaaatcaag aaaatgccag gaacagagaa acacaaagca acaaaccagt
     3661 gacgataatc aggtacagag acatcccttt gtatttgtgc aatgccggta ctttgtaaac
     3721 cagccacgcc gggatcaggc accctaccat gccgaaaatc gggctacaga tagaggtgaa
     3781 gctcaacacc ggtgcgttca gtacgatggc gctccaggcc agcaaaatgg cgaaaatcat
     3841 gatgccgcgc tgaacaagat tttcgttaat cttctcggca ggcatcttgc gacgcaggat
     3901 gttcattacg atcccttgcg ttgcttcgcg aaagcctaaa tagacgccaa agaatgcagt
     3961 catgacagca aagatattga gaatgacgct gaccactttc acccacgctg cgccgtcacc
     4021 gctaataaac tgtgcggcaa tcgccagcgc ggaaatattc tgctcgtagg cttttaccgc
     4081 ttcgtcatgt cccatcgcca gcgtgaacga cacggcgtag aaaaagacgg tgacaaacaa
     4141 aatgccaaac gcgatattca ttgcccgcaa tgctttatgc cgcgccactt caattgattt
     4201 ttcccgcgag cgataagaga tcaccatcgg gcttaacgtc tggataaaca gaatcgacgt
     4261 cagggtaaac ggcagcgtaa taatggcgtt tttcaccagc agccccagcg gcggtagcga
     4321 accgacgtta tacagatgcc acattcccac catcgacacg cccagcgcag cgactaccag
     4381 cagcttggtc agcaccatgt cggtcgaaat tttgaataac aatttctcgc cgcgtgaaga
     4441 gatcgccacc agaatgcaaa tcagtaccag accataaaag ggactgtctg acagcaaccc
     4501 ttccgtcacg ccgaaggtat gcagatagga agcactatcg ttggtgatgg cggtggaata
     4561 gacgaacatc caaatcacca gcattacgaa atagagcgca cctaacagga tgccccagtt
     4621 tttacctaaa taaccgctaa tgacactcgg gtaatcttta cattctggtg attctgccag
     4681 cgtattaata aacaaccgct gaaacagata cattgccggg taaccaatca ccgatgagag
     4741 caaaaatacc cacaatccca tcaaaccgac ctgcaccggg agaaaaacaa tccccgcgcc
     4801 aatcgccatc ccgatactca taatcaccca gccggtgtca gtactgtcga atttgatcgc
     4861 ttctcgccac tcgctctcac tcattccagc acgacccgcc ggggtcgaag cgtctgcaat
     4921 gacgccttta ttcgatgcaa tttccataat ttctcgctca atattttgta gggcttatta
     4981 tttttttccg agccgcatca aggcgatatg cggtctacgt ttttgcaggc gagttattag
     5041 aataaagaaa tgatacgcgc gaggtcagag aaaatcttca caatcaagac tttaaaataa
     5101 cggagatgga taagaatttt ctactaaatt aatcgcaaga gaatattcat tctctatttg
     5161 cggcagatca caaaaaaaag gtgcacattt gtgcacccaa ggatgaaagc tgacagcaat
     5221 gtcagccgca gaccacttta atggccagtc ctccgcgtga tgtttcgcgg tatttatcgt
     5281 tcatatcttt gccggtttca tacatcgtct cgatcacttt atcgagtgaa acacgcggtg
     5341 ccgaggtgcg gcgcatcgcc atccgcgcgg cgtttactgc tttcacggca ttaatggcat
     5401 tacgttcaat gcacgggatt tgtacctgtc cggcaaccgg atcgcaggtc agcccaaggt
     5461 tatgctccat cgcgatttcc gccgcattgc atacctgcgc cggactgccg cccagtagtt
     5521 cagttaaccc tgccgctgcc attgaacagg ccacgccaat ctccccctga cagccgacct
     5581 ccgcgccaga gatggaggcg ttcattttat acagcgcgcc aatagccccc gctgccagaa
     5641 aatagcgggc aattgaccgc tcgtttaccg gacgacggaa cttatcgtaa taagccagca
     5701 ctgccggaat aatgccgcac gcaccgttag tcggtgccgt taccacgcgc ccgccagctg
     5761 cgttttcttc actaaccgcc agcgcgtaca tgttgatcca gtcgatgaca ttcatcggat
     5821 cgttagagat gttatcgcta gaaaccagct gacgacgcag cgctacggca cgacacggca
     5881 cattgagcgg accaggcagc acgccttcag tgttcatccc gcgttcaata ccgtcatgca
     5941 tcacttgcca gatacgggca aaccctgcgt caatttccgc tttgctgcgc agcgccagct
     6001 cgttgtgcat catcaggcca gatatagaca ggccgttgta atcacacatt ttcagcagtt
     6061 cacctgctga gtggaaatcg taaggtacgg acgtttcgac atcgtgcgac aggccgaagt
     6121 gttcttcttc gacaataaac ccgccgccga cagagtaata ggttttactt aatagcgctt
     6181 cctgtccctt ccaggcagtg atccgcattc cgttctcatg gcgaggcaac atttcgggat
     6241 ggaagataat gttctttgct acaggaaaat caacaatatg cgcaccagat gccactggca
     6301 gccgcccgct gcgcgttact aactctataa atgcagggat ctcatcaatg acaacatcct
     6361 gcggactgtt tcctgccagt cccatgataa tggcgacatc cgtggcatgg cctttgcccg
     6421 tcagtgacaa cgacccgtac agatcgacca caatatggct cgtcgcggtt aataagccgc
     6481 tactttccag ccgatcaata aaactttttc cggcattcat tggccccacg gtatgcgaac
     6541 tggagggacc aatcccaatt ttgaaaatat cgaatgcact aatcatatcc acaccctcgg
     6601 attgccgttc agtgaagtgg agcggaacga ccttgcgacc gtcccgctca cgaggcttta
     6661 cgcactacgt actgcgatag cttcaatttc cagcttcaca tctttcggca aacgcgcaac
     6721 ctgcacacag ctccgggtcg ggtaggtcgc ctgatgctca tcgaagaact gcttatagac
     6781 ttcattgatg gtggcaaaat cattcagatc ggtgatgaac acggtcatct tgatgatatc
     6841 gcccacgctc agcccggcag caaccacgat cgctttgacg ttttcgaggc ttaaacgtgc
     6901 ctgatcttgc acatcagccg ggatctcacc ggtctgcggg caaaccggaa tttgcccgga
     6961 ggtgaagacc atgctgccta aatcaacgcc ctgaacataa gggccgattg cgcctggggc
     7021 acgttgcgtt tcgataatct ttttcataca tcctccggcg tcagagcgcc tgggtaaaag
     7081 tacgtgaaat aacatcctgt tgctgttcac gggtcagtgc gttgaagcgc acggcgtagc
     7141 cagagacacg gattgtcagg ttaggatatt tttccgggtg ctcgatggca tccagcagca
     7201 tttcccgatt cattacgttg acgttgaggt gttgaccgcc ttcgacatcc gcttcgtggt
     7261 ggaaataccc atccagcagg ccaacaaggt tggttttacg tactggatct tctttgccca
     7321 gcgccgcagg aacgattgag aaggtgtacg agatcccatc tttggcgtag gtgaacggca
     7381 gtttcgccac cgacgtcaat gaggccacgg cacctttgcg gtcacgacca tgcatcgggt
     7441 tagcgcccgg cgcgaacggt gttccggcgc gacgaccgtc cggcgtatta ccggttttct
     7501 ggccgtacac cacgttagaa gtgatagtca gaatcgactg ggtagggacg gcgttgcgat
     7561 aggttggcag cgctttaatt ttcttcataa agcgttcaac caggtcgcag gcaatgctgt
     7621 ctacgcgctc gtcgttgttg ccgtactgcg gatattcacc gtcgatttca aagtccaccg
     7681 ccaggccgtt ttcgtcacgg attggtttca cgcgggcata tttgatggca gacagggagt
     7741 ccgtcgccac cgacaggccc gcgatgccgc atgccatagt gcgatagaca tcacgatcgt
     7801 gcagcgccat cagcgaagct tcgtagctgt acttgtcgtg catgtagtga atgatattca
     7861 gcgcgctgat gtactgcacc gccagccagt ccatgaagtg atcgaggctg tccatcactt
     7921 tgtcgtaatc cagcacgtcg tccatcagcg gtgctgtttt cggcccgacc tgaatcttca
     7981 gcttctcgtc caccccgccg ttaattgcgt agagcagcgt tttcgccagg ttagcgcgtg
     8041 caccaaagaa ctgcatttgc ttaccaatca ccattgggct gacgcagcag gcaatcgcgt
     8101 aatcgtcgct gttgaagtca gtacgcatca gatcgtcatt ttcatactgc aaggaagagg
     8161 tgacgatcga cacctgcgcg gcatattttt tgaaggcaat cggtaattct tccgaccaaa
     8221 gaatggtcag gttaggttcc ggtgccggcc ccatagtgtg cagggtgtgc aaatagcgga
     8281 aggagttttt ggtcaccagc gtacgaccgt ccagccccat cccgccgatc acttccgtcg
     8341 cccagattgg gtcgccggag aacagcgaat caaattccgg tgtacgcagg aagcgtacca
     8401 tacggatctt catgatgaag tgatcgatca gttcctgtgc ctgctgctca ttgaggacgc
     8461 cagctttaaa gtcgcgctca atgtagatat ccaggaacga tgccgtgcgc cccagcgaca
     8521 tcgcgccgcc attttgcgat ttcactgccg ccagataagc gaagtagagc cactgcaccg
     8581 cttcctgtgc attctgcgcc gggcgagaga tatcaaagcc atatttcgcc gccatttcct
     8641 gaatctgcaa cagcgcatga cgatgctctg ccagctcctc acgcagacgg atggtggctt
     8701 ccagatcctc gcctttttcc agacgagact ggagatcggc aaattgcagt tcgcgttcac
     8761 gcaccagata actgatgcca tacagcgcta cgcggcgata gtcaccgata atgcgcccac
     8821 gaccatagcc atctggtaaa ccggtcagca cgccagattt acggcagcgc agcatatccg
     8881 gtgagtaaac atcaaatacg ccctggttat gggttttacg cagatcggta aacagatatt
     8941 caaattcact gtccatttca cggccatagg cgtggaatga acttttaatc atattaatgc
     9001 caccgaacgg gtgtagcgca cgtttcaacg gcgcatccgt ttgcaggcca acaatttttt
     9061 ccagcggctg gttaatatat cccgcatcat gagcggtaat tgtggtggca atattggtat
     9121 cgaaatcaac cggcgcgtgg gttgcgtttt cgatacggat gccttccatt accttttccc
     9181 acaattccgt ggtggcaggc gtcgcttcgg cgagaaaaga ttcatcgcct tcatacggtg
     9241 tatagttatg ttgaataaaa tcgcggacat taatttcgct tttccagtcc gtacctttaa
     9301 agccaagcca tgcgtcagcg tacagcttat cgctggtatc aatatctacc ttcatgaaaa
     9361 ataatctctc tacaatactt caactaaatt atgcaaattc tgcgggcgcg ttaactttac
     9421 ctaaatgaat ggcatccaaa gcaatcattt tttcttcgtt agtcggaata acgacgcaaa
     9481 tgacatgcgc attttcactg gaaacaattc gctcgccaaa agagttagat cgattattca
     9541 tttctgtatc aatctctacg cctaatacag ccaaatgttc catgaccaga cgacgaatta
     9601 aacttgaatt ctctcctatt ccgccggtga atataattcc atccagccga cgtaatgaag
     9661 ctgcatgtcc ggcaatatga cgggcaattc ggtgaacaaa ggttttaatt gccagttgcg
     9721 cgcgttcgtg accttcatgc caggcttttt ccagaacacg taaatccgaa gaaagaccgg
     9781 aaatacctaa taatcccgac tctttattca ctacgcgttc caggtcaccc aggctctggt
     9841 tggtttggct ggcgacccag gacatcgcac caaagtcgac atcgccactg cgggtaccca
     9901 tcatcaagcc ttccagcggc gtcattccca ttgaggtgtc aacactctga ccgttgcgaa
     9961 ctgcgcagat tgacgcgcca ttgccaagat gcgccacaac caggccggag tcatcttccg
    10021 ccagattcag cagcgaatgt gcgcgctggg aaacatagcg gtgcgacgtg ccgtggaaac
    10081 cataacggcg tacacctaac tcttcataat atttccacgg caggccgtat aaataagctt
    10141 ccggagccat cgtctggtgg aaactggtat caaataccgc cacctgagtt acgcccggaa
    10201 ataattgctg cgccgattca ataccactta aatttgcgta attatgcagg ggtgccagtg
    10261 gagaaacgcg acggatatta tcaatgactt catcggtaat aatggcggac tcggtaaaaa
    10321 tactgccgcc gtgagcgatg cggtggccaa ttaaggccac gctgtcattt aaattccgtt
    10381 tttccagttc aaatgcaatt gccttcaatg caccttcgta gctgtggtga gccagcggtg
    10441 ctggctctcc cccatttacg gataagaatg cattttccga gttaataccg tcggcaatac
    10501 ctgacattaa tacttcacaa tcgctggcat cgagtacgga aaacttaatc gaagacgaac
    10561 cacagttaat aaccaaaaca accggaaatt cattcatctc ttttctcatc ctgagttacg
    10621 gattaaaaca gtttgtatac gatgttcagg atggtcagca gaccaatcac ggtaacaaac
    10681 acgttatcca gacgaccacg gtatttcgcc agagacggcg ctttacggat agcatacatc
    10741 ggcaacaggc acagcaggga tgcgataatc ggtgcgccca tggcttcaat caggtcgagg
    10801 atgttcgggt tggcgtaggc aacaacccag gtggagccca tgatgaagat catgctgaga
    10861 gtattcagtt tacccagcga cactttggtt ttgtcacctt tataaccgaa cttcagaatc
    10921 agaccattca agccttccag cgtccccaga tagtgaccga agaaagattt gaagatagcc
    10981 acgagtgcga tgatggaagc cgcatattcc agtgtaatcg cgaacgttgt tttcgtaccg
    11041 gtcatggacg caaagtggtt agccagataa gaaagcactg gaatattctg cgctttggct
    11101 tccgccatgt tggccggaga cagagtaaac aggcagctaa aggcaaagaa catcaccact
    11161 gcaaccatca gcatgctggc acgagaaatg atttgggaac atttacgttc ggtgaagtcg
    11221 cgaccgaagt ctttctcata ctcttcacgt ttagaaacca cgaaggaaga gacgattggc
    11281 gagaagttaa aggagaaaac catgatggaa atccccagcc agacagtgat caggataccg
    11341 tcatgaccgg ttaacgacag cgaaccgagg tcaacctggt cgataactgc agagttccag
    11401 taagggatca gcgacagaga aatcagcacc aggctggcga taaacggcca taccaggtag
    11461 ctcatcactt taaccatcag atccttacca aaccagatga cgaaagccat cagcagcaac
    11521 aggaacagcg ccacaaagcc gcgattcagc ggtgcaaatc cgagctggtt ttcccagaac
    11581 gtcataaagg tattggtaat agtaacgcca taaatccaca gcagtgggca aatcgcgaag
    11641 aagtacagga acgtgataac cacgccgcca gttttaccaa aatgctcttc caccgtttcc
    11701 gtaatgttgc cggaagggtt agagccagaa agacacagac gcgccagcgc ccggtggcaa
    11761 taaaatgcga tggggtatgc caataccaac atcagaagaa tcgggatcag tccgccaaaa
    11821 cctgcgcgga tagggaagaa cagcaccccg gcgccgattg ccgtaccaaa caagcctaac
    11881 gtccatgtgg tatctgattt acgccaggac gattgttttg tctggctgga tacaatgcta
    11941 tctgaagtac tcatatccta tcctcaacga attaattaag cgtcaacgaa accggtgatt
    12001 tgagagacgc gagaaagatc gatattgccg ccggaaataa tactgacggt ttttctgttt
    12061 tgaatatatt ggtctaattt accgcttaat aatgcagcac atgccagagc gcctgcgcct
    12121 tcggtgacga ctttattgcg ctgaattaag gcaatcatac tgtttctgat ttcgtcttcg
    12181 ctgaccagca cgatgtcatc gactaattca cgaacgattt cgtaagttaa attacccggg
    12241 cgggagacat cacaaccatc cgccagggtg ccggtagttc ggtgcgtggt tatttctccg
    12301 gagtggaaag aagccgccat gccgtgaacg ttttctgact gtacgccaat aacacgaatg
    12361 gtcgggttaa tagatttaat tgccaccgca ataccagcaa ttaaaccacc accaccaatt
    12421 ggcacaatca cgttatcgac atcatagaga tcttccataa tttccagacc aatcgttccc
    12481 tggccagcaa tcactttcgg atcatcgtaa ggtgggataa aaatacgacc ttccatttcg
    12541 acaatttcgc tcactttagc gatagtgtcg ttgaagttat caccatgcag aacgacttct
    12601 gcggagtagt cgcacgttgc cgctactttg gattttggcg caccttttgg catcaccact
    12661 ttaccgtcga tacccagcat cgcgcaggag agcgaaaccc cttgcgcatg gttgcccgca
    12721 gaacaggcca ccacgccttt gcgtttttcc gcatcggtca gtgaacttaa tttattaaat
    12781 gcgccacgaa ttttaaatga acccgtacgc tgcatatttt caaacttcag gaatatttca
    12841 cctttgcaac gttcactaaa atagttggag cgaggcatgc ctgttttata aattcgccca
    12901 gccagtcgtt gtttcgcttc aataatatca tcaatagcaa ccggcagatc gtatgtaata
    12961 tgcattataa aacctcttcg cctgaattaa atatgtaggt aaccgtaacc gacacctgca
    13021 aaacagacag gtggattatt tatgtttaaa taaaaacaaa caactaacca acttctatta
    13081 attgccttcg tctacaccca ttataagatg aatactcttt ggctaattcc accaaaaccg
    13141 atgctgcttt tttaatacga taatttttcg accataccgc ggcatattgt gccacaggta
    13201 atgtttcttc aaccggaata gtaataaatt gattagaacc aaaaggtgac gtcatatcac
    13261 aaggaattac agttaagaaa tcagcattga gaacaagatt ataaattgtc acgactgagt
    13321 cggttttaac gatgttttca atactgatgc catttctttg taacgtagta agcagttcgc
    13381 tgtagtaccc catattagtt tgtggcaaca cccactgttc gttcttcaac gactccagcg
    13441 tggtggtgcc ggtgcatgtt cgggacttac tggctaccag cacaaactcg gactcgaaca
    13501 gcggctcaac atgtaaatcc tgaagcttca tttctgcact taacgtacca atcgcaaaat
    13561 ccagtcgacc gtcgcggatt gccggtaaga acgaagacag ttgcgcttca tacatagaaa
    13621 cctgcgcttt cgggaacacc tctttgaact tgttgatcat ccctgacata aaagtaaaac
    13681 caatcaatga aggaaaacca aatgagactt ccaccaccgc ttcagaagac ataccgctta
    13741 tctcattaac catatttttc atttcacggg taatggattc ggaacgggag agtaacaatt
    13801 gaccggcagg tgttaatgtt acaccggtat ttttccgcac cactaattcc acaccaaaat
    13861 aatcttcaat atcgttaatg attttactga cggccggttg agttaaccct aattcttttg
    13921 cagccgagcc gatagagcca cttctaatga cttcctgaaa gactaccagg tgctgcgttt
    13981 tcggaagaag aatagtgctc ataacgacct acgttaatta cctcattgac ggcatgaagt
    14041 gtatcaaaat gaaataaaca ggatatgtgc gaccactcac aaattaactt tcattacttt
    14101 ccaaggtatc gttattacaa ttaaatatct ttaaatatca acaagttaaa gcataaaaat
    14161 cagcataaac cctgattttt atcaaaaaaa aatgacgggg ataaccatat tttatggcga
    14221 taacatcatt cgttatgtgg ttaatacaaa aaaggggctg agaccatatt tcaagcaatt
    14281 accggataat taccaggcaa aatttgagtt aaatttaatg agcaagttct caaattttat
    14341 tataaataaa ccattttcag cgataaatac tgcggcacgt cacatttttt cacgctattt
    14401 gttggagaac aaacatttat tttatcaata ttttaaaatt tcgaatacag gtattgatca
    14461 tctcgaacaa ttgattaacg tcaacttttt ctcttctgac aggacgtcat tttgtgaatg
    14521 caatcgtttt ccataaattc ttctcccctc ataggcgacg aatagcattt tgtgttgagg
    14581 atcacaaaac gaataattgc tggtcgccgc gataaggtca gacgaagtca acaaggggga
    14641 ttttcactgt gctttacatc agcgtaggac acagaatgtt gcttcgttta ctgtaacgcc
    14701 gggtaaatga gcgttttttg atagtgcgta agtgtcgtgc gaggaaaata agcatctatt
    14761 attgtttgtc atgacgacaa ggcaggcatc aatgaaaggc ttcccaattg cacatatttt
    14821 tcacccttca atcccgccaa tgcacgcagt ggttaacaat cacaatagaa atattgatta
    14881 ttggacggta aaaagaaagt ttgcagaaat tgtctccacc aatgacgtta ataaaattta
    14941 cagtataagt aatgaactcc ggagagtatt atctgcaatt actgcattga atttctatca
    15001 aggcgatgtt ccttctgtca tgatccgaat ccaaccggaa aatatgagtc cattcatcat
    15061 agatatttct acaggagaac atgatgatta tatcatacaa acattagatg taggcacttt
    15121 tgcacctttt ggtgaacaat gtacttgctc agccgtcaat aaaaaagagc tggaatgtat
    15181 taaggagacg atttctaagt attgcgcaaa gttcacccaa aaagaagcca tcttaacacc
    15241 ccctaggcac tttaataaaa cctgcattac ttcagattgt tggcaaattc tctttttttc
    15301 acccgatcat tttaataatg atttttattg attatttata aaggataaaa taatgttccc
    15361 tgtttcatca attggtaatg atattagtag tgatttagtc cgtcgaaaaa tgaatgacct
    15421 tcctgaaagc cccataggaa ataacctcga agcactagca ccaggtatag aaaaactaaa
    15481 acagacctct attcaaatgg tcactttact taacgcgtta caaccaagtg gaaaatgcat
    15541 tatcactggt gattttcaaa aagaattagc gtacttacaa aatgtaattc tttataatga
    15601 ctcgtctctt cgtctggatt ttttaggtta taacacccaa attattcaac gatcggacaa
    15661 tacctgcgaa cttaccatta atgaaccgtt aaaaaactag gaaatatcta caggcaatat
    15721 caatgttaat tgcccattaa aagatattta caatgaaatc aggaggttga acgtaatctt
    15781 tagttgtggg actggagata tagttgatct atcctctctg gacttacgta atgtcgattt
    15841 agattattat gatttcacag ataaacatat ggctaatact attctaaatc attttaaatt
    15901 ggattcaaca aattttacta atgccaacat gtttcaggtt aattttgtta gttcaacaca
    15961 aaacgccaca atctcctggg attatttact aaaaataacg cctgttttaa taagcattag
    16021 cgatatgtat tctgaagaaa aaatcaagtt tgtcgaaagt tgtttaaatg agcttggaga
    16081 cattaccgaa gaacaattaa aaattatgag atttgcaatt ataaaatcta taccaagggc
    16141 aactcttaca gataaattag aaaatgaatt aacaaaagaa atatataaaa gctcatcgaa
    16201 aatcaacaat tgcttgaaca gaattaaatt accagagatg aaagaattct catcaaaaaa
    16261 aatatatgat tacatcgata caatcattga agattatgaa aatatcaaag aaaatgctta
    16321 tctggtcgtc ccccaaatta attatactat ggatttaaac atagaagact ctagctcaga
    16381 agagttaata ccctcgagaa agacgaaaat tctccggaca atggctttga ggtcggggaa
    16441 tataacacat atgaagcata taactcagag aatcaatatt ttaccagaga ggactatacg
    16501 tataattacg accttttaaa tgcaatatag acttaataac ttcttacaaa gtctattatt
    16561 aataccgtag atattcatct taccctgtgc accaaaagca aaaacttatc gacgcgggtt
    16621 tgaaactgat ggcaaccgca aaaatgcctg atgcgctacg cttatcaggc ctacgccatc
    16681 tctgcaatat attgaatttg cgtgcttttg taggcaggat aaggcgttca cgcagcatcc
    16741 ggcatgaata aagcgcactc aacaatctga aacccgccgg aacggtttat tacgtacatc
    16801 aggtgaaact gaccgataag ccgctttctt ttgggtatag tgtcgtggac agtcattcat
    16861 ctttctgccc ctccaaaagc aaaaacccgc cgaagcgggt ttttacgtat atcaggtgaa
    16921 actgaccgat aagccgcttt cttttgggta tagcgtcgtg gacagtcatt catctttctg
    16981 cccctccaaa agcaaaaacc cgccgaagcg ggtttttacg tacatcaggt gaaactgacc
    17041 gataagccgc tttcttttgg gtatagcgtc gtggacagtc attcatcttt ctgcccctcc
    17101 aaaagcaaaa acccgccgaa gcgggttttt acgtaaaaca ggtgaaactg accgataagc
    17161 cgggttctgt cgtggacagt cattcatcta ggccagcaat cgctcactgg ctcaagcagc
    17221 ctacccgggt tcagtacggg ccgtacctta tgaaccccta tttggccttg ctccgggtgg
    17281 agtttaccgt gccacggact gttaccagcc gcgcggtgcg ctcttaccgc accctttcac
    17341 ccttacctga tcccgcttgc acgggccatc ggcggtttgc tctctgttgc actggtcgtg
    17401 ggtttccccc ccaggcgtta cctggcaccc tgccctatgg agcccggact ttcctcccct
    17461 ccgcccgtct cccccgaaga ggacgacgac gaagcggcga ctgtctggtc agcttcggcg
    17521 cgcagtatag agggtttgcg cgcccttgtc accccgtgtt gcgcattcca atcgccagtg
    17581 tcgcggcaat attacgtgaa gcacggcaga tattgtcata agccccgcgg aatgcttcgt
    17641 ccaacgtacc tatgctggtc aatacgctga agaccgcatc aatgccatgc tgatgtacaa
    17701 cgccaacatc atcggtcagg ctacccgcaa tgccaatcac cggtttatgg tacttcttcg
    17761 ccacgtttgc gacgccaatc ggtaccttgc cgtgaatact ctggctgtca atacgccctt
    17821 caccggtgat caccagcgta caatcgtgaa tatgttcctc cagattcagc gccgtagtga
    17881 cgatttcaat accacttttc agttccgcac caagaaacgc catcagcacc gcacccatac
    17941 cgcccgcagc tcctgcgccg gggacatctt ttacatcaac atgcagcgct tttttaatga
    18001 catcggcata gtgagagagg ttattgtcca gctcaacaat catcgcttca ctggctccct
    18061 tttgtgggcc aaagatgcgc gatgcgccgt tatcgcccac cagcggattg gtgacatcac
    18121 aagcgacgcg aatgacgcaa tcttttaagc gcggatcgag gccggaaata tcaatatcat
    18181 tcagagtatt aagactaccg ccgccaaaac caatttcatt gccgttggcg tcgcataatt
    18241 tcgcccccag cgcctgtacc atgcctgcgc cgccatcatt tgtagcgcta ccgccaatgc
    18301 cgataataat gtttgtcgcg ccgctctcca gcgcctacag gattaactcg cctgtgccgc
    18361 gtgaagtggt cacgagtgga tcgcgttttt ccgcaggtac cagctccagc ccactggccg
    18421 ccgccatttc aataaacgcg gttttgccat cgccggagat cccccaactg gcattcactt
    18481 tctcgcccag cggccctgta acccaggcgt gacgttcaga accctgggtg gctgcaatca
    18541 tcgcttccac cgttccttcg ccaccgtcgg caaccggaat agaaacgtac tgtgcatcag
    18601 gaaaaatttc ccgaaatcct ttttctatcg cctgcgcaac ctcgctggca gataaacttt
    18661 ctttataaga gtctggggcg attacgattt tcatacctat gcctgttacc acatgacgcc
    18721 ggagggcgtt tctcttattc ggcctggatt ccaggcccgg attgcaatac gccatccggg
    18781 cacgacgtca ttaacgagta acttcgactt tcgccagttt ttcgtagtag cacgccaggg
    18841 cgctatgatc cgccgttcct aaaccatctg ctcgcagtgc ctgcatcatc tccataaccg
    18901 cagctgtgag cggcagttgc gcgccgacgc cgtgggaagt atccagcgca tttgccagat
    18961 ccttaatatg cagatcaata cggaagcccg gcttgaagtt gcggtccatc accatcggcg
    19021 ctttggcatc cagcacggta ctgcccgcca gtccaccgcg aattgcctga tacaccaggt
    19081 ccgggttaac gcccgcttta gttgccagcg ttaacgcttc tgacatcgcg gcaatattca
    19141 gcgccacaat gacctgattc gccagtttgg tgacgttacc tgcaccaatt gccccggtat
    19201 gcaccacgga acccgccatt gctttcatca aatcatagta tttgtcgaaa atagccttgt
    19261 cgccgcccac catcactgac agcgtaccgt cgatggcttt cggttcaccg ccgctcaccg
    19321 gagcatccag catatcaatg cctttcgctt tcagcgcttc gctgatttca cggcttgcca
    19381 gcggtgcgat agaactcata tcgatcaata ccgtacctgg cttcgcgcct tcaataatgc
    19441 cattctcacc cagcgccacc tctttcacat gaggggagtt tggcagcatg gttatgatga
    19501 cgtcgcactg ttcagcgatc gctttagccg tagaggctgt ttctgcacct gcagcaatca
    19561 cgtcagcaat agcttctggg ttacggtcag caaccaccag cgagtaacct gctttcagaa
    19621 ggtttttact cattggttta cccataatcc ccaggccaat aaaaccaact ttcatagtca
    19681 tatcaatcat ctctcttgtt gcggtggtgg ttatttttta aaggtatcag ccagtttatg
    19741 agtggcagag cggaagacgc cgagatcgct gccgacagcc acaaacgtcg cgccccattc
    19801 cagataacga cgcgcatcgg cttcgaccgg cgcgaggata ccgctgggtt tgccgtgcgc
    19861 gctggcacgg ttaaaaatgt gctgaattgc tttttgtaca tccgggtgtg atgcattgcc
    19921 gagatggcct aatgccgcgg ccagatcgct ggggccgacg aagatgccgt ctacgccttc
    19981 ggtagcggca atggcatcga cgttatctac gccctgctga ctttctatct ggaccagaat
    20041 agtgatgttc ttgttcgact gagcgaaata atccgccacg gtgccaaaca tattggcgcg
    20101 gtgagaaacg gagacgccgc gaatgccttc cggtgggtaa cgggttgatg ccaccgcctg
    20161 ctctgcttcc tcttttgttt ctacaaaagg aatcaggaag ttatagaaac cgatatccag
    20221 aagacgctta ataatttccg gctcgttggt cggcactcac actactggcg cgctggcgct
    20281 gcctttcaag gccattaact gcggaataaa cgtggagata tcgtttggcg catgttcgcc
    20341 atccagcacc agccagtcaa acccagccaa accaagaact tcagtgctaa tcgggttaga
    20401 gagtgctgac cagcaaccaa tttgtacctg tttcgcagcc agtgcggctt tgaatttatt
    20461 cgggaaaaca tcgttattca tcgcttatac ctttgcttat ttctgcaatt ccatacgttt
    20521 aatgtcgcca actacgaaga ggtagcagac catcgccatc agcgctgaac atcccacgaa
    20581 aaccagtgct gcattgaagg agtgcagttc acttaccagg tagccaatca ccagtggagt
    20641 gacaatggag gcaacattgc caaagacgtt aaagacgccg ccacagaggc caacaatctc
    20701 tttcggcgcg gtgtcagaaa tcaccggcca gcccagagca ccaaatcctt tgccaaaaaa
    20761 agccagcgcc atcagcatga ccaccagcgt ggtgttgttg gtgtagttac ataagatgat
    20821 ggtggaagcc agcaacattc ccagcacaat cggtagctta cgtgccaggg tcagggataa
    20881 accgcgtttg atcagataat ccgagaagac acctcccagc acgccgcccg caaaaccaca
    20941 cagtgccgga atcgaggcga ccagacccac tttcagaatc gacatgcctt tttcctgcac
    21001 cagataaatc gggaaccagg tgaggaagaa ccaggtgatg gtgttgataa aatattgtcc
    21061 gaaaaatacg cccaacatca tgcggttaga gagcaattgc ttgatgtaat gcagtttgag
    21121 accgcttgct gccgcgctgc ccggcttttt gtggtccata tcgaccaccg cgccattttc
    21181 agagataaac ttcagctctt cctcagacat acgtgggtga tctgtcgggt tatgaatcaa
    21241 cttgatccac agcgccgtca gcacaaaaca aatcaccccc ataacggtaa agacgtgctc
    21301 ccatccccag gcgaaagtca gccagccaag cagaggcgaa aagagcgcca gcgagaaata
    21361 ttgtgccgag ttaaagatgg cggaggcagt accacgttct ttcgtcggga accaggcggc
    21421 gacaatacgg gcgttcgccg ggaatgatgg agcttccgag aatcccagca taaagcgcat
    21481 aaagaacatg gagatccctg cccaggccag cgggaacata tcaacaaagc cttgcaggaa
    21541 ggtgaacagc gaccagaaaa agaggctgta ggtgtaaact tttttcgagc caaacttatc
    21601 aagcaaccag ccgccgggga tttgcatcag caagtaggcc cagccaaaag cggagaagat
    21661 gtaacccatc gaaaccgcac ttaactgcaa ctcttttgcc acttcggtac cagcaataga
    21721 cagcgttgca cgatcggcgt agttaacggc ggtaacaata aaaataatca gtaatattaa
    21781 atagcgggta tgcacacctt tccttttttc gtcaacggtg tccagaatca ttttatttac
    21841 ctcgggtact tatgctgatt tttattatta tggggaaggt gttatttata agtttcattt
    21901 atgccgtaac gacaatgaac tcgggaatta gtataagcag cgcgagaata ataatcattg
    21961 tgcaaatgct aatttaatta atacttttta aatattattt tgagcatatg cacataaggt
    22021 tgcgcgctaa agcacagatt tgcgctttac cttgccgggc ggcactgcaa tccctgaaat
    22081 gattgacatt gatcacattt ctgcgtttaa actcctgaca ttcttatttc acccaatgaa
    22141 atcatttatt tttaaatgag accaggtcct cattttaata acccctggct ggagaatatt
    22201 gcacaatggc caacatcgaa atcagacaag aaacgccaac tgcgttttat ataaaagttc
    22261 acgacacaga taatgtggca attattgtta atgataatgg cctgaaagca ggaacgcgtt
    22321 ttccggatgg gctggaatta attgaacata ttccccaggg gcataaagtc gcattgctgg
    22381 acattccggc taatggtgaa attattcgtt atggcgaagt gattggttac gccgtgcgtg
    22441 caatcccacg cggaagctgg atcgacgaat caatggttgt actaccggaa gcgccgccgt
    22501 tacacacgct gccactggca accaaagtcc cggaaccctt accgccgctg gaaggataca
    22561 cctttgaggg ctatcgcaat gccgatggca gcgtgggcac caaaaacctg ctcggcatca
    22621 ccaccagcgt ccactgtgtg gcaggcgtgg tggactatgt agtaaaaatc attgaacgcg
    22681 atctgctacc gaaatacccg aacgtcgatg gcgtggtggg gctgaatcat ttgtacggtt
    22741 gtggcgtggc gattaacgca ccggcagcag ttgtacctat tcgtaccatt cacaatattt
    22801 cgctgaaccc taactttggc ggcgaagtaa tggtgattgg cctgggttgt gaaaagttgc
    22861 agcctgagcg cctgctgact ggaacggatg atgtgcaagc tattccagta gaaatcgcca
    22921 gcattgtcag tttgcaggat gaaaagcatg tcggttttca gtccatggtc gaggatattt
    22981 tgcaggtcgc cgaacgccat ctacaaaaac tgaatcaacg tcagcgagaa acctgtccgg
    23041 cttcagaact ggttgtcggc atgcattgcg gtggcagcga tgcgttttct ggcgtaacgg
    23101 caaacccggc agttggctat gcgtctgatc tactggtgcg ctgcggcgca acggtgatgt
    23161 tttcagaagt cacggaagtg cgtgacgcga tccatctgct gacaccacgc gcagtgaacg
    23221 aagaggtcgg caaacggctg ctggaggaga tggagtggta cgataactat ctcaatatgg
    23281 gaaaaaccga ccgcagcgcc aacccttcgc cgggcaacaa gaaaggcggt ctggcaaacg
    23341 tggtagagaa agcgctcggc tccattgcta aatcgggcaa aagtgcaatt gttgaagtgc
    23401 tgtcgcccgg tcaacgcccg actaaacgcg gattaattta cgccgcgacg ccagccagcg
    23461 attttgtctg tggcacgcaa caggtggctt cgggtatcac cgtgcaagtg tttacgaccg
    23521 gtcgtggtac gccgtacggc ctgatggcgg tacccgtcat taaaatggca acccgcaccg
    23581 agctggcgaa ccgctggttt gatttaatgg atattaatgc gggcaccatc gctaccggcg
    23641 aagaaactat tgaagaggtg ggctggaagt tgttccactt tattctcgac gtcgccagcg
    23701 ggaagaagaa aaccttctcg gatcaatggg gactgcataa ccagctggcg gtgtttaacc
    23761 cagcaccggt gacctgataa ttcaattcgt attatgcccg atagcacacg tttatcgggc
    23821 atattgagtc acagcgaaga cgcgtgatcc ccgaccagaa tcacttcaac cccagccttt
    23881 cgcagtcctt ccagactatc cgcaggaatg ccttcatcaa caatgatcat gtcgatacgt
    23941 tgagtatcaa tgatcttatg taaactggaa cgattgaatt tactggaatc ggtgaccacg
    24001 atgatccgtt ccgcaacttc gcacatccga cggtttaaac gggcttcatc ttcattatgt
    24061 gtgctgacgc cgcgctccag atcgatcgca tctacaccaa gaaacagcat atcgaagtgg
    24121 taattttgca gcgattgctc agcctgatcg ccgtaaaaag attgcgactg acggcgcaaa
    24181 tgcccgccgg tcatcagcag ctcaacgcct tccgcttcca gcaacgcatt agccacgttc
    24241 ataccgttgg tcatcgcaat tacgtcagtg tacttgcgca tcagacgagc aatctcaaaa
    24301 gtggtggtcc cggaatcgag gatcacccga tgacctggct gaatcaactc aacggcagct
    24361 ttcgcaacgc tgcgtttcat cgcggtgttc agtgcgcttt tatcttccac tgatggctcg
    24421 actgacggcg tcgtgctatc gcagatcaac gcgccaccat aggcacgcac agcgatcccc
    24481 tgcttttcca gaaacgccag atcattgcgg atcgtcacag tagatacgcc atacaatgcc
    24541 gacagatcgt taacctgcac actcccttgc tgtcgcagac gctgaatgat ctgttctcgt
    24601 cgctcgctgg tgcctgtcac tcgcttctca cctgaagcgt cggtattact catagtaagt
    24661 cctttcgtaa aactttcgtt tcatttcgtt ttgcctatta acgcctttct attaagcaaa
    24721 tgcaagccca ccttgcccat tggcgcaagc tactctcgtt tcagtgactt tcattacgtt
    24781 tcttttgtga atcagatcag aaaactatta tctttcgttt tatttttatc tcaccatgac
    24841 gcagtatcaa ttgaaacaaa acgaaagatc aatatcgcag taatctgaac tggagaggaa
    24901 agtgaaacat ctgacagaaa tggtgagaca gcacaaagcg ggcaaaacaa atgcaattta
    24961 tgccgtttgt tccgcacatc cgctggtgct ggaggctgca atccgctacg ccagtgcaaa
    25021 ccaaacgccg ttactgattg aagcaacctc caatcaggta gaccagttcg gcggttatac
    25081 cggaatgacg cccgccgatt ttcgcggctt tgtttgtcag ctcgccgact cgttgaattt
    25141 cccgcaggat gcgctgattc tgggtggtga ccatctgggg ccaaaccgct ggcaaaacct
    25201 gccagccgct caggcaatgg ccaatgccga tgatttgatt aaaagctacg ttgcggcagg
    25261 attcaaaaaa atccaccttg attgcagcat gtcctgtcag gacgatccaa ttcccttaac
    25321 tgatgacatc gtggctgaac gcgccgcccg tctggcgaaa gtggcggaag aaacctgtcg
    25381 cgaacacttt ggcgaagccg atctggagta tgtcattggt accgaagtgc cggtacctgg
    25441 cggcgcgcat gaaaccttaa gcgagctggc ggtcaccacg ccggatgccg cccgcgccac
    25501 gctggaagcc catcgtcacg cctttgaaaa gcaaggtttg aatgccatct ggccacgcat
    25561 tattgccctg gtggttcaac ccggcgtcga attcgatcac accaacgtta ttgattatca
    25621 gcccgccaaa gcgagcgcct taagccagat ggtcgaaaac tacgaaacgc tgattttcga
    25681 agcgcactct accgattatc aaacgccgca atctctgcgc cagctggtga ttgaccactt
    25741 tgccattctg aaagttggcc cggcgctgac cttcgccctg cgtgaagccc ttttttctct
    25801 ggcggcgatt gaagaagaac tggtaccagc gaaagcctgt tccggtctgc gtcaggtgct
    25861 ggaaaacgtg atgctcgacc gcccggaata ctggcaaagc cactaccacg gtgacggcaa
    25921 cgcgcgtcgt ctggcgcgtg gttacagcta ctcagatcgc gtgcgctatt actggccgga
    25981 cagccagatt gatgacgctt tcgctcatct ggttcgtaat ctggcggatt caccaattcc
    26041 gctaccgctc atcagccagt atctgccgct gcagtacgtg aaagttcgct ccggcgagct
    26101 gcaaccgacg ccacgggaac tcattataaa ccatattcag gacatcctgg cgcagtacca
    26161 cacagcctgt gaaggccaat aagcaaaaca aagaggaaca tgctatgcca aatattgttt
    26221 taagccggat tgatgaacgc ttgattcacg gtcaggtcgg cgttcagtgg gtcggatttg
    26281 cgggggcaaa tctggtgctg gtagccaacg atgaggttgc cgaagatccg gtacaacaaa
    26341 acctgatgga aatggtactg gcagaaggga tcgccgtacg tttctggacg ctgcaaaaag
    26401 ttatcgacaa cattcatcgc gccgccgatc gacagaaaat cctgctggtt tgtaaaacac
    26461 ccgccgattt cctgacgctg gtgaaaggtg gcgttccggt gaatcgcatt aacgttggca
    26521 atatgcacta cgccaatggc aaacaacaaa tcgccaaaac ggtttctgtg gatgcgggcg
    26581 atatcgcagc atttaacgac ctgaaagccg ctggggtgga atgcttcgtt cagggcgtcc
    26641 cgacagagcc tgctgtggac ctctttaaat tactttgagg gattcatcat ggaaatcagc
    26701 ctgttgcagg catttgcgtt gggcattatc gcctttatcg ctggcctaga tatgtttaac
    26761 ggcttaaccc atatgcaccg cccggtggtc ctcggcccgt tggtcgggct ggtacttggc
    26821 gatctgcata ccggaatttt aaccggcgat acgctggaac tagtgtggat ggggctggcc
    26881 ccgctggcgg gcgcacagcc gcctaacgtg attatcggta ctatcgtcgg cacggcgttt
    26941 gccattacta ctggcgtgaa acccgatgtc gcggtcggtg tcgccgtacc tttcgctgtc
    27001 gcagtacaga tggggattac cttcctgttc tcggtgatgt ctggcgtgat gtctcgctgc
    27061 gaccggatgg cggagaacgc cgatactcgc ggcattgaac gcgtgaacta tctggcactg
    27121 ctggcactcg gcactttcta ttttctctgc gctttcctgc ctatctactt cggcgcggaa
    27181 catgcaaaaa ccatcattga tgtcctgccg caacgattaa tcgacggcct cggcgtcgcg
    27241 ggcggcatca tgccagcaat cggttttgcc gtgctgctga aaatcatgat gaaaaacgtc
    27301 tacataccct acttcatcct gggctttgtt gccgcaacct ggctcaagtt accggtgctg
    27361 gctattgctg ccgccgcgct ggcgatggcg ctgatcgatc tgctgcgtaa atctcctgaa
    27421 ccgactcaac ctgcggcaca gaaagaggaa tttgaagatg gcatctaatc aaaccaccct
    27481 gccgaacgtc agcgaaaatg aagaaacact gctgactggc gtcaatgaaa acgtgtatga
    27541 agatcggagc attgccgcgg agctgacgaa aaaagatatc aaccgcgtcg cctggcgttc
    27601 catgctgtta caagcgtctt ttaactacga acgtatgcag gcttctggct ggttgtacgg
    27661 tctgctgcct gctctgaaaa agatccacac taataaacgc gacctggcgc gcgccatgaa
    27721 ggggcatatg ggtttcttca atacccatcc gtttctggtg acgtttgtta tcggcattat
    27781 ccttgcgatg gagcgttcta agcaggacgt taacagtatt cagagcacca aaattgccgt
    27841 cggtgcgccg cttggcggga ttggcgatgc gatgttctgg ctaacgctac tgccgatttg
    27901 tggcgggata ggcgcaagcc tcgcgctaca aggctctatt cttggcgcag tcgtctttat
    27961 tgtgctgttc aacgtggtac acttggggct gcgttttggt ctggcgcatt atgcttaccg
    28021 catgggcgtg gcggcgattc cccttattaa agcgaatacc aaaaaagtcg gccatgctgc
    28081 gtcaatcgtt ggaatgacgg taatcggcgc actggtggca acctatgttc gtttaagcac
    28141 cacgctggaa atcacggcgg gcgacgcagt agttaagtta caggctgatg ttatcgacaa
    28201 actgatgcct gccttcttac cgctggtcta caccctgacc atgttctggc tggtacgccg
    28261 cggctagagt ccgctgcgcc tgattgcggt taccgtggtt ctagggatcg tcggtaaatt
    28321 ctgccatttc ctttaaatac aatgaggtat tagatgttaa gtattatttt gacagggcat
    28381 ggcgggtttg ccagtggcat ggaaaaagca atgaagcaaa tcctcggcga tcaatcgcag
    28441 tttattgcca tcgattttcc cgaaacctca agcgccgcgc tgcttacatc gcagcttgaa
    28501 gaggccatcg cccaactgga ttgtgaagac ggcattgttt ttctgacgga tttgctgggc
    28561 ggcacgccgt ttcgcgtagc ttcaacgctg gcgatgcaaa aaccgggctg tgaagtgatt
    28621 accggcacca atttgcaact gctactggag atggtgctgg agcgcgaagg gttaagcggt
    28681 gaagaattcc gtgtacaggc gctggagtgt ggacaccgag ggctgaccag tctggtggac
    28741 gagttaggcc gctgccatga agaatgtccg gtcgaggaag gaatatgaca cacgttctgc
    28801 gcgccagaag gctgctgact gaagagggat ggttcgatga ccatcagttg cgtgttgctg
    28861 acggtgtcat cgcagcaatc gaaccgattc cagtgggcgt gactgaacgc gatgcggaac
    28921 tgctctgccc cgcttacatc gacacccatg tacacggtgg tgcgggcgtt gatgtaatgg
    28981 atgacgcgcc ggatgtactc gacaagctgg caatgcacaa ggcacgcgaa ggtgtcggca
    29041 gttggttacc gaccaccgta accgcgccac ttaataccat tcatgcggcg ctgaaacgta
    29101 ttgctcaacg ttgccaacgc ggcggacctg gtgcgcaagt gctggggagt tatctcgaag
    29161 gaccgtactt cacgccgcag aataaaggcg cgcatcc
//
LOCUS       DAEEGA010000050        28406 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEEGA010000050 DAEEGA010000000
VERSION     DAEEGA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28406
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.050"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="KLH46_002344"
     CDS             complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="KLH46_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HBE0379000.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            323..1954
                     /gene="dcuS"
                     /locus_tag="KLH46_002345"
     CDS             323..1954
                     /gene="dcuS"
                     /locus_tag="KLH46_002345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBE0379001.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRIFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1951..2670
                     /gene="dcuR"
                     /locus_tag="KLH46_002346"
     CDS             1951..2670
                     /gene="dcuR"
                     /locus_tag="KLH46_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBE0379002.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3241..4581
                     /gene="dcuB"
                     /locus_tag="KLH46_002347"
     CDS             3241..4581
                     /gene="dcuB"
                     /locus_tag="KLH46_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBE0379003.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4659..6305
                     /gene="fumB"
                     /locus_tag="KLH46_002348"
     CDS             4659..6305
                     /gene="fumB"
                     /locus_tag="KLH46_002348"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBE0379004.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPVFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6428..7057
                     /locus_tag="KLH46_002349"
     CDS             6428..7057
                     /locus_tag="KLH46_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBE0379005.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7196..8617)
                     /gene="melB"
                     /locus_tag="KLH46_002350"
     CDS             complement(7196..8617)
                     /gene="melB"
                     /locus_tag="KLH46_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBE0379006.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8732..10075)
                     /gene="melA"
                     /locus_tag="KLH46_002351"
     CDS             complement(8732..10075)
                     /gene="melA"
                     /locus_tag="KLH46_002351"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBE0379007.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPS"
     gene            10358..11266
                     /gene="melR"
                     /locus_tag="KLH46_002352"
     CDS             10358..11266
                     /gene="melR"
                     /locus_tag="KLH46_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBE0379008.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11465..13732
                     /gene="adiA"
                     /locus_tag="KLH46_002353"
     CDS             11465..13732
                     /gene="adiA"
                     /locus_tag="KLH46_002353"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBE0379009.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14057..14818
                     /gene="adiY"
                     /locus_tag="KLH46_002354"
     CDS             14057..14818
                     /gene="adiY"
                     /locus_tag="KLH46_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBE0379010.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14955..16292
                     /gene="adiC"
                     /locus_tag="KLH46_002355"
     CDS             14955..16292
                     /gene="adiC"
                     /locus_tag="KLH46_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBE0379011.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16396..18039
                     /gene="eptA"
                     /locus_tag="KLH46_002356"
     CDS             16396..18039
                     /gene="eptA"
                     /locus_tag="KLH46_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBE0379012.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRATNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18036..18704
                     /gene="basR"
                     /locus_tag="KLH46_002357"
     CDS             18036..18704
                     /gene="basR"
                     /locus_tag="KLH46_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBE0379013.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18705..19805
                     /gene="pmrB"
                     /locus_tag="KLH46_002358"
     CDS             18705..19805
                     /gene="pmrB"
                     /locus_tag="KLH46_002358"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBE0379014.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="KLH46_002359"
     CDS             complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="KLH46_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBE0379015.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19982..21484)
                     /gene="proP"
                     /locus_tag="KLH46_002360"
     CDS             complement(19982..21484)
                     /gene="proP"
                     /locus_tag="KLH46_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBE0379016.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21748..22626)
                     /locus_tag="KLH46_002361"
     CDS             complement(21748..22626)
                     /locus_tag="KLH46_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBE0379017.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="KLH46_002362"
     CDS             complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="KLH46_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBE0379018.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25253..25588
                     /locus_tag="KLH46_002363"
     CDS             25253..25588
                     /locus_tag="KLH46_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBE0379019.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25746..26189
                     /gene="yjdN"
                     /locus_tag="KLH46_002364"
     CDS             25746..26189
                     /gene="yjdN"
                     /locus_tag="KLH46_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBE0379020.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26322..27110
                     /gene="phnC"
                     /locus_tag="KLH46_002365"
     CDS             26322..27110
                     /gene="phnC"
                     /locus_tag="KLH46_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBE0379021.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27135..28151
                     /gene="phnD"
                     /locus_tag="KLH46_002366"
     CDS             27135..28151
                     /gene="phnD"
                     /locus_tag="KLH46_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0379022.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28257..>28406
                     /locus_tag="KLH46_002367"
     CDS             28257..>28406
                     /locus_tag="KLH46_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HBE0379023.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATF"
BASE COUNT         7297 a         7421 c         6934 g         6754 t
ORIGIN      
        1 acgactttcg tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct
       61 gacatatcgc agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc
      121 cgtccagtag cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag
      181 catagccccg gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta
      241 tatagccaat catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga
      301 aatttgtcac acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg
      361 tccgatgaaa ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct
      421 attggtggtg catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc
      481 caacaaggca ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt
      541 gcagaaaaaa ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacgcaa
      601 cgatctgctg tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc
      661 ccagcgtatt ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga
      721 aaatgtcgct atcaatcgcg gttttctggc gcaggcttta cgcatattta cccccatcta
      781 cgatgaaaat cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac
      841 ccaacagatc aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt
      901 cggactgatt ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga
      961 accctacgaa atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga
     1021 aggcgtcgtt gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga
     1081 attgctgaat taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg
     1141 gtcacaggtg gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga
     1201 gattacgatt aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt
     1261 tatcatcggt gccatttcaa ccttcaggga caaaactgaa gttcgtaaac tgatgcagcg
     1321 actcgacggt ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa
     1381 taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta
     1441 cattctcaaa acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa
     1501 atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca
     1561 tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc
     1621 gacgctgatt accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga
     1681 acccggtggc gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt
     1741 taacgatgat ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt
     1801 ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa
     1861 tctcggcggc agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca
     1921 gataccctgg gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac
     1981 gcaatggtcg cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt
     2041 ggaacagcct cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc
     2101 gacctgatat tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc
     2161 ctgcataacg cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca
     2221 accattaaag attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct
     2281 tcccgctttg aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat
     2341 cagtattatg accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag
     2401 gatcctcgtc gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg
     2461 attgacgcgc atcaggacta tgagttttca accgacgagc tggctaacga ggttaacatc
     2521 tcgcgtgttt cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc
     2581 agtatccatt atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac
     2641 tactcactgc tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt
     2701 attaccgtaa tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc
     2761 cagatcaaat ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg
     2821 gtgtttttaa tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg
     2881 tgtattttta cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc
     2941 tgagaacaaa aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat
     3001 ctgcgcagta caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt
     3061 atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc
     3121 taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac
     3181 agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac
     3241 atgttattta ctatccaact tatcataata ctaatatgtc tgttttatgg tgccagaaag
     3301 ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc
     3361 caccttcagc caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg
     3421 gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag
     3481 ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg
     3541 accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc
     3601 gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag
     3661 atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt
     3721 aatgtcacct ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca
     3781 tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg
     3841 gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac
     3901 ggtgataccg cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg
     3961 attttcctcg gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca
     4021 tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc
     4081 ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc
     4141 ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc
     4201 atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat
     4261 ccgtgggcgt atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg
     4321 gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg
     4381 gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca
     4441 gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt
     4501 tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc
     4561 gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca
     4621 ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct
     4681 accaggcacc tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt
     4741 acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagcactga
     4801 ccctgctggc gcagcaagcc tttcacgacg cttctttcat gctacgtccg gcacaccaga
     4861 aacaggttgc ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac
     4921 tgcaattctt aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata
     4981 ccggcaccgc gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg
     5041 aagaagcgct gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc
     5101 aaaacgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa
     5161 tcgacctgta cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg
     5221 gctctgccaa caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac
     5281 tgaaaaactt cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc
     5341 atatcgcgtt tgtgattggc ggtacgtctg cggaaaccaa cctgaaaacc gtcaaattag
     5401 caagcgctca ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc
     5461 gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc
     5521 agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg
     5581 catcctgccc ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa
     5641 tcaaccgcga aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac
     5701 aagaactgcg tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga
     5761 aagagatcct cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca
     5821 ccattattgt gggtcgagat attgcacacg ccaagctgaa agagctgatt gacgccggta
     5881 aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc
     5941 ctgccggtta tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg
     6001 tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc
     6061 agcaggttac cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc
     6121 cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc
     6181 tgggtatgga agctatctgg aaaatcgaag tagaagattt cccggtgttt atcctggtcg
     6241 atgacaaagg taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta
     6301 agtaacctct tcggcccagc gcctgacagc atgctgccag gtgatccccc tgggccacct
     6361 cttttgcgat tgtaatttca tgcttgctgg tgaatagtca gtattttccc tgacttgcga
     6421 actcaccatg acccgcacac tcaagccgtt aattcttaac accggcgcac tggcgctaac
     6481 gttaatcctg atttataccg gcatttcggc ccatgacaaa ctcacctggc tgctggaagt
     6541 gacaccggtg attatcgtcg tgccgctact acttgccacc gccagacgtt acccgttaac
     6601 gctgctgctc tatacgctca tcttctttca cgccatcatc ctgatggtag gcgggcagta
     6661 cacctacgcg aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagtcgtaa
     6721 tccgtatgac aagctggggc actttttcca ggggctggtg cctgcactgg tggcacgaga
     6781 aattctcgtt cgcgggatgt acgtgcgcgg acgtaaaatg gtggcgtttc tggtctgttg
     6841 cgtggcgctg gcgataagcg cgatgtatga attaatcgag tggtgggcgg cactggcgat
     6901 gggccaggga gcggatgatt ttctcggtac ccagggcgac cagtgggaca cgcaatccga
     6961 tatgttctgc gcgctgcttg gcgcattaac gacagtgata ttgctcgctc gctttcattg
     7021 ccgccagcta cggcgctatg gcttgataac tggataacaa cgtgcacttt gttgatgccg
     7081 gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa
     7141 gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg
     7201 ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt
     7261 tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca ttgaggcgat
     7321 agaaacggaa gtagagaatc agcgttacca tgaaaaacag agtcggtaga gcaatcataa
     7381 taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca
     7441 tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag
     7501 tctgcacgga gtaagcgatg ctttcacagc gtacgtgcag tttatattca ccgtaatcaa
     7561 cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca
     7621 aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga
     7681 gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata
     7741 atgatttaac taagcgtggg aagaatacta acgtcaccag gttagcagct cccgcatacg
     7801 acagataata ggggaacaaa tccgcatcac cgataacata tgagaaataa tagatagcaa
     7861 atccggtaat aatgttgctg gctacattat aagcaagagc catacccaag aggcatgaaa
     7921 gctgatcgtt tttataaata agcgcaacga tggctttaag tgtcagatgg cttccttcag
     7981 cagacgattg attgtctgac gaaaagactt catgcacatt gcgcagagtg atgatggttg
     8041 aaacaataaa aaaggcgatc aataccagag tgaacatctg aaagccaaat ccccgatcgc
     8101 caccgccgac ataattaaca aatggtagcg tcacacctgc cgtaacaaag cctgccagac
     8161 tggcaaaaaa acgcggataa ggaaccagtt gttcgcgttc acgtttatcg agcgtgatgg
     8221 ttggaaccag cgaccagaag ggaatatcca taatggtgta agtcatgccc cagaggatgt
     8281 aggtcacgca aacaaagaca atctgagtag taccttcaaa cagatgcgca ctaaagagga
     8341 gaaataagat tacagagttt gccaacgtac cgatcaggat ccagggtttg aacttacccc
     8401 atcgcgatcg cgtagcattt acaatccatc ccataatcgg atcgttaata gcatcccaga
     8461 tcctcgccac cagaaacaaa gtaccgacca aacccacaga cagcccgacg acatcggtgt
     8521 agtaatacat gaggtacata tacacaatgc cgatcgcaaa atccttcccg aacgctccaa
     8581 atccataact gagttttgta gtcattgaaa tgctcatagg gtatcgggtc gctgttacca
     8641 acgcccgcct tctgttattg gtatcgtcag aggtgtgcgc cggaataaat cccccggcgc
     8701 agtagcgttt agtcgcgttt taacggtgca actagcttgg cagccagtcg ccgtgggcgg
     8761 caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg gcagtatgcg
     8821 ggtccatcat cgcggcgtgg taaacacggt cgcgattttc cgtaagaata gcttcggtca
     8881 gcagcgtctg tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtagcgtac
     8941 cgactttggt cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc
     9001 cttgtggcag gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc acactcggct
     9061 cgccagtcca gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa
     9121 tccgggaggc gtttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc
     9181 agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgaccta
     9241 gcttaataaa ccacggcgta tattcggcaa aatgttctga cgattccgtg acgaagtagc
     9301 ccagcttttt gaacatttcg tagcgcacaa tattctggca gcgagtatta ccatgaatat
     9361 ttggcttcgg tgcctgccct gcttcataag ccgccagcag ttccgggtag agattcacgt
     9421 aactgccgtc ggcggttttg cgctccagct ccaggtaaaa cgccatatgg ttgatacctg
     9481 cgcaacggta acgcagcgta gccgggtcga tattgaggtc acgcgccagc tcttccgccg
     9541 ttccctgcac cgaatggcac agcccgacct gtttgatata cggatagcgg gcatacatcg
     9601 cccaggtatt catcgccatt gggttaacat agttgagcat ggtggcatcg gggcagactt
     9661 ccgtcatgtt ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac
     9721 cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga
     9781 aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg
     9841 catcctctaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg
     9901 aatccatcag cttacgcacc acaatatgcg actcttccag gcgggtgggg tcaatgtcca
     9961 tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca agaatatttt
    10021 taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga
    10081 tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccggct
    10141 tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt
    10201 atgggaataa attacctgaa aagtagagtt ttcggcctca tgatgctcgg acagtcccct
    10261 cgcccctctg ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct ccctcactcc
    10321 tgccataatt ctgatattct aggaaagaga gccatccatg aatacagata cgtttatgtg
    10381 cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg tattcggaat accagcggat
    10441 ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga
    10501 agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat
    10561 caatcagggt catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg
    10621 aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct
    10681 ggataaagac ctgattaacc acgtcactca cggcatggtg atcaaatcac ttgccacaca
    10741 gcaacttagc ccgtttgaag tacgccgctg gcagcaggaa ttaaacagtc cgaatgagca
    10801 aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctag
    10861 ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca
    10921 tgcgcaattt tatgttagcc agatgctggg ctttattgcc gaaaactatg atcaggcgct
    10981 gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt
    11041 ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt
    11101 tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt
    11161 tcgttcaagt agtcgttttt acagcacgtt cggcaaatat gtcggcatgt cgccgcaaca
    11221 ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta
    11281 tcagccaaaa aaatagttta gccgtcgata ataagcagtg aaaataattt aaaataatca
    11341 cataacgtat tgtttataaa actaaatatt tcaaaaatgt ttgtttttca cgcgctttac
    11401 agcccgaaaa ggccggaaga tacttgcccg caacgaagat tccttcataa ccgggtaagc
    11461 aatgatgaaa gtattaattg ttgaaagcga gtttctccat caagacacct gggtcggtaa
    11521 cgccgttgag cgtctggcag atgctttaag ccagcaaaat gttaccgtga ttaaatccac
    11581 ctcctttgat gatggttttg ccattctctc ttcaaacgaa gccattgact gcctgatgtt
    11641 cagctatcaa atggagcatc cggacgaaca tcaaaacgtc agacaattga tcggtaagct
    11701 tcatgagcgc caacaaaacg tgccggtctt cctgttgggc gatcgggaaa aagccctcgc
    11761 cgcaatggac cgggatctgc tggagcttgt cgatgaattc gcctggattc tggaagatac
    11821 cgccgacttt atcgccggac gcgccgttgc cgcgatgacc cgctaccgcc agcagctgtt
    11881 gccgccactg ttcagcgcgc tgatgaaata tagtgacatc catgaatatt cctgggcagc
    11941 gccaggccac cagggcggcg ttggttttac caaaacaccc gccggacgtt tctaccatga
    12001 ctactatggt gaaaatctgt tccgcaccga catgggcatc gaacgaactt ccctcggttc
    12061 tttgcttgac catactggcg catttggcga aagcgaaaaa tatgccgcac gcgtatttgg
    12121 tgccgatcgc tcctggtcgg tagtcgtcgg tacttccggc tctaaccgca ccatcatgca
    12181 ggcttgcatg accgataacg atgtcgtggt cgttgaccgt aactgccata aatccatcga
    12241 acaaggtttg atgctgacag gcgcgaaacc ggtctatatg gtgccaagcc gcaaccgcta
    12301 cggcattatc gggccaatct atccgcagga aatgcaacct gaaaccttgc aaaagaaaat
    12361 cagtgaaagc ccgctgacca aagacaaagc cgggcaaaaa ccgtcttact gcgtggtaac
    12421 caactgcacc tatgacggcg tgtgttataa cgctaaagaa gcgcaggatc tgctggaaaa
    12481 aacctccgat cgtctgcact ttgacgaagc ctggtacggc tatgcacgtt tcaacccgat
    12541 ctatgccgat cactatgcca tgcgcggcga acctggcgat cacaacggtc ctaccgtttt
    12601 cgccacccac tccatccaca aactgctgaa tgcgctgtca caggcttctt atattcatgt
    12661 acgtgaaggt cgtggggcga ttaacttctc ccgcttcaac caggcctaca tgatgcatgc
    12721 caccacctcc ccgctgtatg ccatctgcgc atccaacgac gtggcggtgt cgatgatgga
    12781 cggcaacagc ggcctgtcac tgacacagga agtgattgac gaagcggttg atttccgtca
    12841 ggcgatggcg cggctatata aagagttcac cgctgacggt agctggttct tcaaaccgtg
    12901 gaacaaagaa gtcgtcaccg acccacaaac cggcaaaacc tatgactttg ctgacgcacc
    12961 aaccaaactg ctgaccaccg ttcaggactg ctgggtaatg catccgggcg aaagctggca
    13021 cggcttcaaa gatattccgg ataactggag tatgctcgac ccaattaaag tcagcatcct
    13081 tgctccggga atgggtgaag atggtgaact ggaagaaacc ggtgttccgg cggcgctggt
    13141 cactgcctgg cttggtcgcc acggcattgt gcctacccgc accactgact tccaaattat
    13201 gttcctgttc tctatgggcg taacccgtgg gaaatggggg actctggtta acaccctttg
    13261 ctccttcaaa caccactatg acgccaacac accgctggcg caggtgatgc cggaacttgt
    13321 tgaacaatat cctgacactt acgcgaacat ggggattcac gatctgggtg acaccatgtt
    13381 tgcctggctg aaagaaaaca accctggcgc acggttgaac gaagcctatt ccggcctgcc
    13441 ggtggcggaa atcaccccgc gtgaagcgta caacgcgatt gtcgacaaca atgtcgaact
    13501 ggtatccatt gaaaatctgc caggacgcat cgcggcaaac tcagttatcc cgtatccgcc
    13561 aggaatcccg atgctgctgt ctggtgaaaa cttcggcgat aaaaacagtc cgcaagtaag
    13621 ttatttacgc tcgctgcaat cctgggacca ccatttccct ggatttgaac acgaaactga
    13681 agggactgaa attattgacg gtatttacca cgttatgtgc gtgaaagcgt aaccactatt
    13741 ccgctgaagg cgtaattgtt taaataacat tacgccgcct ggccttaggc cttttgagta
    13801 tggcaacgtt ttcataaaaa ttgctgcaaa caaaaatgtc atactttttg cgcggcccca
    13861 ccccgcgctt ttttttgcct gttatttatc ctgtaaaaaa atatgtacat gagaaattac
    13921 tataaaaatt tgtaatatta gtaaaactcg ttatttttat gtatgtttat attcatcatg
    13981 caattatata accatttccc ggtatcgctt tgctttagcg agaactggtc ttttattcgc
    14041 actcaggagt acatgtatga ggatttgcag cgaccaacct tgtattgttt tattgactga
    14101 aaaagatgtc tggataaggg tgaatgggaa agaacctatt agccttaaag ctaaccatat
    14161 ggcgttatta aattgtgaaa ataatattat cgacgtctcc tctcttaaca acactttggt
    14221 tgctcatatt agtcacgaca tcatcaaaga ttacctccgg tttctgaata aagatctctc
    14281 gcaaatacca gtatggcaac gtagcgctac gcccatactc accctgccat gcctgacgcc
    14341 agacgtcttt cgcgttgccg cgcaacacag catgatgccc gcagaaactg agtcagaaaa
    14401 ggaacgaaca cgcgcattat tattcacggt gctatcccgt tttctcgaca gtaaaaaatt
    14461 cctttcatta atgatgtata tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat
    14521 tgaaagcgat attcacaaag actggaatct tagtatggta gccagttgtt tatgtcttag
    14581 cccaagtctg ttaaagaaaa agttgaaaag cgaaaacacc agttatagcc aaataatcac
    14641 cacctgccgc atgcgttatg ccgtaaatga attaatgatg gacggtaaaa atatctctca
    14701 ggtatcacag tcctgcggct acaacagtac gtcgtacttt atttctgtct ttaaagactt
    14761 ctacggtatg acgccgctgc attatgttag tcagcacaga gaacgcactg tcgcctgatt
    14821 tttaacctta acgaagagct atattaataa cggcatcagc gataacccgg tcgataataa
    14881 ttcaactatc gaatgcaggc gtatgatatg acgtaattta ttgtcacgaa gctcgccttc
    14941 gcaggagttt aattatgtct tcggatgctg atgctcacaa agtgggctta atccccgtca
    15001 ccctgatggt gtcggggaat attatggggt caggtgtttt tctgttacct gcaaacctgg
    15061 cctccactgg cgggattgcc atttatggat ggttggtgac gattatcggt gcgctggggc
    15121 tctcgatggt atacgccaaa atgtcgttcc tcgacccaag tcctggtggt tcttacgctt
    15181 acgcccgccg ctgctttggc ccgtttctcg gttatcaaac caacgtcctc tactggctgg
    15241 cctgctggat cggcaatatc gccatggtgg tcattggcgt aggatattta agttacttct
    15301 tcccgattct gaaagatcca ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct
    15361 tcgtcctgct gaacattgtc ggtccgaaaa tgatcacccg cgtgcaggcc gttgccactg
    15421 tgctggcgct gatccccatc gtcgggattg ccgtatttgg ctggttctgg ttccgtggtg
    15481 aaacctatat ggcggcatgg aacgtcagcg gcctgggcac cttcggtgcg attcaaagta
    15541 cccttaacgt tacgctgtgg tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg
    15601 tggtgaaaaa cccgaaacgc aatgtcccta tcgccaccat tggtggggta ttgattgccg
    15661 ccgtttgcta tgtgctctcc accaccgcga ttatggggat gatccctaat gccgcactgc
    15721 gcgtttctgc ctcgccattc ggtgatgccg cacggatggc gttgggtgac accgctgggg
    15781 ccattgtttc cttctgcgca gctgcgggtt gcttaggttc actgggcggc tggacgttgc
    15841 tggcgggtca aacggcgaaa gccgctgccg atgacggact gttcccaccg atttttgccc
    15901 gtgtaaataa agcgggtacg ccagtggcgg ggctgattat cgtcggtatt ttgatgacca
    15961 tcttccagct cagcagcatt tcaccaaacg cgaccaaaga gttcggtctg gtttcttccg
    16021 tctcggtcat ctttacactg gtgccatatc tttacacctg tgcggcgtta ctgctgctcg
    16081 gccacggtca ctttggtaaa gcacgcccgg catatctggc tgttactacc attgccttcc
    16141 tctactgcat ttgggccgtg gtggggtccg gggcgaaaga ggttatgtgg tcatttgtca
    16201 ccctgatggt catcaccgcc atgtatgcgc tgaattacaa ccggctacat aaaaacccgt
    16261 atcccttaga tgcaccaata agcaaagatt aattcccctt aatccagcaa acataaaagc
    16321 caaccttaag aacttaaggt tggcttaatt ttgctttgcg agcatatgcg cactttgttc
    16381 gatggaaaca ccgtgatgtt gaagcgccta ctaaaaagac cctctttgaa tttactcgcc
    16441 tggctattgt tggccgcttt ttatatctct atctgcctga atattgcctt ttttaaacag
    16501 gtgttgcagg cgctgccgct ggattcgctg cataacgtac tggttttctt gtcgatgccg
    16561 gtcgtcgctt tcagcgtgat taatattgtc ctgacactaa gctctttctt atggcttaat
    16621 cgaccactgg cctgcctgtt tattctgatt ggcgcggctg cacaatattt cataatgact
    16681 tacggcatcg tcatcgaccg ctcgatgatt gccaatatta ttgataccac tccagcagaa
    16741 agttatgcgc tgatgacacc gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct
    16801 gcgctgattg cctgctggct aaaaatcaaa cctgccacct cgcgtctgcg cagtgttctt
    16861 ttccgtggag ccaatattct ggtttctgta ctgctgattt tgctggtcgc cgcactgttt
    16921 tataaagact acgcctcgtt gttccgcaat aacaaagagc tggtgaaatc cttaagcccc
    16981 tctaacagca ttgttgccag ctggtcatgg tactcccatc agcgactggc aaatctgccg
    17041 ctggtgcgaa ttggtgaaga cgcgcaccgc aacccgttaa tgcagaacga aaaacgtaaa
    17101 aatttgacca tcctgattgt cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc
    17161 tacccgcgtg caactaaccc gcggctggcg aaagataacg tggtctattt ccctaatacc
    17221 gcatcttgcg gcacggcaac ggcagtttca gtaccgtgca tgttctcgga tatgccgcgt
    17281 gagcactaca aagaagagct ggcacagcac caggaaggcg tgctggatat cattcagcga
    17341 gcgggcatca acgtgctgtg gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc
    17401 gtgcctcacc agaacgtcac cgcgctgaat ctgcctgatc agtgcatcaa cggcgaatgc
    17461 tatgacgaag tgctgttcca cgggctggaa gagtacatca ataacctgca gggtgatggc
    17521 gtgattgtct tacacaccat cggcagccac ggtccgacct attacaaccg ctatccgccg
    17581 cagttcagga aatttacccc aacctgcgac accaatgaga tccagacctg taccaaagag
    17641 caactggtga acacttacga caacacgctg gtttacgtcg actatattgt tgataaagcg
    17701 attaatctgc ttaaagaaca tcaggataaa tttaccacca gcctggttta tctttctgac
    17761 cacggtgaat cgttaggtga aaatggcatc tatctgcacg gtctgcctta tgccatcgcc
    17821 ccggatagcc aaaaacaggt gccaatgctg ctgtggctgt cggaggatta tcaaaagcgg
    17881 tatcaggttg accagaactg cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac
    17941 aatttattct ccacgctatt gggattaact ggcgttgaga cgaagtatta ccaggccgcg
    18001 gatgatattc tgcaaacttg caggagagtg agtaaatgaa aattctgatt gttgaagacg
    18061 atacgctgtt attgcaggga ctgattctgg cggcgcaaac cgaaggctac gcgtgcgatg
    18121 gcgtgacaac cgcgcggatg gcggaacaaa gccttgagga cggtcattac agcctggtgg
    18181 tactggattt agggttaccc gacgaagatg gactgcattt tctcgcccgt atccggcaga
    18241 aaaaatacac cctgccggta ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg
    18301 ccgggctgga tgtcggtgcc gatgactatc tggtgaagcc ttttgcgctg gaagagttac
    18361 acgcccgtat ccgcgccctg ctacgacgcc ataataatca gggcgaaagt gagctgattg
    18421 ttggcaatct gacgctgaat atgggtcgcc gtcaggtatg gatgggcggt gaagagttga
    18481 ttctgacgcc caaagaatat gctctgctgt cacggttaat gctcaaagca ggcagtccgg
    18541 tacatcggga aattctctac aacgacatct ataactggga caatgaaccc tcgaccaaca
    18601 ccctggaagt gcatatccac aatctgcgcg acaaagtggg caaagcccgt atccgcaccg
    18661 tgcgcggctt tggctatatg ctggtcgcga atgaggaaaa ctaattgaat ctgatgcgtt
    18721 ttctgcgccg accaatatcg ctgcgccaac ggctgatatt gaccatcggg gccattttgt
    18781 tggtgtttga gctgatcagc gtcttctggc tatggcatga aagtaccgag cagattcagc
    18841 tgtttgagca ggcgctacgc gacaatcgca acaacgatcg ccacatcatg cgtgagatcc
    18901 gcgaggcggt cgccagcctg attgtccccg gcgtctttat ggtcagcctg acgctattta
    18961 tctgctatca ggcggtacgc cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg
    19021 aagcgcgcac cgccgacaac ttaacgccca ttgccattca cagcgccacc ctcgaaatcg
    19081 aagcggtggt ttcggcgttg aacgatctgg tcagtcgcct gaccagcacg ctggataacg
    19141 aaaggttgtt taccgctgac gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt
    19201 tgcatctgga actgctggcg aaaacgcatc acattgatgt agcaccgtta gtggcacggc
    19261 ttgatcagat gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat
    19321 tttcttccgg taattatcaa catgtaaaac tgctggaaga tgtcattctc ccctcgtatg
    19381 acgaactcag taccatgctc gaccagcgac agcaaaccct gctattgcca gagagcgccg
    19441 ccgacatcac cgttcagggt gatgcaaccc tgctgcggat gttattgcga aacctggtag
    19501 aaaacgccca tcgttacagc ccgcaaggca gcaacattat gattaagctg caagaagatg
    19561 gcggagcggt catggcagtt gaagatgaag gaccaggtat tgatgagagt aaatgcgggg
    19621 agttgagtaa agcgtttgta cgtatggaca gccgttatgg cgggattggt ctggggttaa
    19681 gtattgtcag ccgcattaca cagttgcatc acgggcagtt tttcctgcaa aaccggcaag
    19741 agacttccgg cacgcgggcc tgggtacggc tgaagaaaga tcagaacgtg gcaaaccaga
    19801 tataaagaaa gctgctgacc accagcacgc tgaacacggt agttaaactt tcataaacac
    19861 ggtttttcat tgcactctcc tccgttaacc tggaggagag tatgcgcgtc agagattaag
    19921 tgaaccttaa gagttcaatt ggcctgataa gacagcgtca catcaggcca tccgtttcag
    19981 cttattcatc aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg
    20041 caatctcgtg gtcaatatta tcgattttct gctcgatatt atcgtaatgc tcgacgagaa
    20101 tttccttcgc ttcctgtata tctgacgccg ccggtgtcgc acctttcaac ggacgatttg
    20161 ccgtctcttt catggttacg ccggtgatta aaccaatcac cgccactacc atcaggtaat
    20221 aggcaggcat catcagattc tgcgagcttt cgaccagcca ggccgccagt gttggcgtca
    20281 gaccggcaac cagcaccgaa atattaaatg ccgccgccag cgcgctgtaa cggatatgcg
    20341 tcgggaacat cgctggcaag gtagaagcca taacgcccat aaagcagtta aggatcaccg
    20401 ccagcatcag taacccggca aaaatcaggc cgatgacgtt actgttaatc agaataaacg
    20461 ccgggatcgc caacacaaac agtgcaacac taccaagtag cacaaacgga cgacggccaa
    20521 aacggtcact cagcaagccc atcaccggct ggacaaacag cataccgatc ataatggcga
    20581 taataatcag caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa taactcggca
    20641 tataggtcag caacatgtag taagtcacgt tggtggcaat taccagacca atacatgtca
    20701 acaggctgcg ccagtattta gtggcaatct ctttaaacga gactttcggg ccatcctgca
    20761 aaccttcgcg gtcgccctgt tccagtttat caacatgctg ctggaacgcc ggagtttctt
    20821 ccagcgcatg gcgcaggtaa agcccgataa tccctaacgg cagagcaata aagaacggaa
    20881 tacgccagcc ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt aacaccacca
    20941 cgcccgcacc cagcacaaac ccggcaatag aaccgaagtc cagccagctg cccataaagc
    21001 cacgtttacg gtcaggggag tattccgcaa caaatatcga cgccccggta tattcaccac
    21061 cgaccgagaa accttgtgcc atcttacaga tcaacagcag aatcggtgcc caaataccaa
    21121 tcgtgtcgta ggacggtatt aagccaatac agaacgtact gatcgacata atcacaatag
    21181 tgatagcgag gatcttctgg cgaccatatt tatcgcccaa cataccaaag aagagtccgc
    21241 caagcggtcg aatcagaaag ggaacggaga aagtggcaag tgcagcaacc atctgcacgc
    21301 tggggtcagc ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa ccataaacac
    21361 caaaatcaaa ccattccatt gcattaccca gtgatgctgc ggtaatggct ttacgcagtt
    21421 taccgtcatc aataatggtg acatcacgaa gggtaatcgg ttttactttt ttccttttca
    21481 gcatagcttt cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg
    21541 gccgcaaaag gggagcggga attgcaggat gcaatctctg taacaagcgt agcgggtttg
    21601 cttacaccct ccggttaagg aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt
    21661 aatgaccttt ttaccctacc agtgtttaaa tttgtgatca acttcacaca tggatttcgt
    21721 ttaggactca ttgatgtaac tgattattta cgccaaattt acagagaaga tttcctcgac
    21781 cattgccccc accaaacgct gcgctgaaca gagtcgtggc atccccagcg cgacagtttg
    21841 ccagcgttgg gtcactgacc agcaaacggg ttgtttatct gccgtggtcc actttcccag
    21901 ccaacttaaa aaatcagcat gatccccgcc cgtcggaacg gtcgaagagg caaaccactg
    21961 atggtaatag tgacgcgtag tcggtgccgc attgatactc tctgccagat gttgtgtcgc
    22021 tttctcgcgc aaatcaccca gcaacaacac caccgcttgc gggcttccca gacggcaggc
    22081 atcgccaaat gcaccccagc gcaactcctc cagaacgata aaacagcgcc caggcaacga
    22141 ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt tgctcgctct gatcacctgc
    22201 ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc cagcgacaac gggctgaaaa
    22261 ccactcatac aaggcggcaa tttgctgggg gagctccggg aaagcagagt gctggcggtg
    22321 atccattttt tgcagcagcg ccagatgctg cgcgatctgc gtcagcgcca gatgaccggg
    22381 atccagcatc gacgccagtt tttccaccat tgtcagtcgc aggctaagcg gcgcatttaa
    22441 catgccgccg ttcgtccatg tctgaagctg cacacgcgaa agaagttcct gcattagtcg
    22501 ttcacgaaac tgctgctgat gggcttgagg atggcgggca tcatcgccct gcgccagatc
    22561 cactaaaaag cgcggatgaa ccgactccag cacgcgaccg gggccgtcca gtaacgtctt
    22621 ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat cgcgtaacag ctgagcgtcg
    22681 gtgtaaatcc agctgtgagt catcacagtc tgctgcaatt gctgctgcat cagcctgacc
    22741 accgattcat tttgttgacg cagagccaga ctttcgcgta aacgcgtctg taattctgtc
    22801 aaacatgaag cgaactcagc gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc
    22861 tgctctgcca gaactttagc catttgttgg caatagagat cgacttctgc gcttaatgct
    22921 cgttgcaaca cactgtaatc aaccgtttct gtcggggatt tctcatttcc ccgtccccag
    22981 tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa cgcctaaacg cgtgctctgc
    23041 tcgtccgttg cttcctggcg cgaaatgact tcactgaaca gctggcgagt gttgaagtgg
    23101 ggaactacgc cgtgaaaaac aggaaaatga aacccaggac gaaaccctga ctcgctcaat
    23161 tccattttga cttgttgctc aatggggcga ataacatcgg ttaacacccg gcaaagggtg
    23221 gattccatct cggcaaaacg cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt
    23281 aacagtgtct cacagcgggt acgaatctcg cttaacggct ccgaatcatc ctgaaacaag
    23341 gcggctaact gcgcattcac cgcatcttgc tgttgacgca gaaagtggtt ggcggaggtc
    23401 agggccagct cgatttcatg tttaatctcg ccgctcacct gcgcctgatt gagttgcaat
    23461 agctgcaaac tttcttcgac ctgatggata ttttgccgca attgttcaca agcgacgttt
    23521 aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct gcgcgtaatt caacagttta
    23581 tgcgcagcag atcgcagagc atacaatgag gcgttagcgt aagcggcatg aagcaacgcc
    23641 tgaattggct gggcgaacag cgaatcttcc cacaactgat cggcagcatg acgaatatgt
    23701 tcgaggtccg ccagatcggc atgacgccag cgcctgccga gcgcggcatg ggcaaaatct
    23761 tccacccagc gttgttgctc tggcgctggt aacttaccgt tgttggctaa ctcatagcgc
    23821 gcccgattcg ccaggtagcc ccacatcgac gacaccggga atatctgctg tggcgtaata
    23881 cagcctttca tcagcgtccc ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta
    23941 cgatcctgtt gatcgaactt attgaccagc acatacagcg gcaccgattg ccccaccgcc
    24001 aaaatcgcct cacggacctc ttcatcggag atcgatttca gttgcgtata atccagcacc
    24061 gccagtaccg ccgaggcgcg tgccagctgc tggttaagca ttttttgcag atgcggttgc
    24121 ccggcttcat ttggcccggg ggtatccagt aacgtcaact gaccgggata actttccagc
    24181 cccgccagat ggacaaactc cacttcaatc acgggaatat gctcaatggc ggcgtaagca
    24241 gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca ctaaatcatt caaacttttc
    24301 agacaatgaa aaataggctg ggcacccaga taatattttt cgaaagcgac gccattttcg
    24361 atccgctgca taagcgcacg catatcttta tctatttcca gcacatcggt cagatgctta
    24421 atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta aacaatcgat tggcgcgaca
    24481 tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat ggcgaataag cgtcggcagc
    24541 gcagtcattg ggcgattacg attaggcaga acctccgtac caacaatggc attaatggtg
    24601 gttgatttcc ctgctttcat ggtaccgaca attgccagca ccatttccag tcgggaaatt
    24661 ttacacaact cattattcag catcgcgtga cgttcggcga tattaggctg actccagggt
    24721 aaagccagtt gtggcgcgtc gtctccgggt acagagagag gcattttttc cagtaactgc
    24781 aactgttggc gagaaagctg taacaggcgt tcagcctcct gacttaactc atacagggtc
    24841 tgtgtgtaca tagaaaattc ttccttaaag caaattttgt tacttttatt tagccagatt
    24901 gtttttgagt tctgttttcg gcttttataa ttactgcaag aaataatttt atatttagtg
    24961 tgttgttttt tatcagaata aataacgtct tctgatgcgt ttaaaacgtc agaaagataa
    25021 aaatatcatg tgaataaaaa aaagaacaag tagagcatta acattatctt aaataataaa
    25081 tagaggcaaa aagattattt tctttttacg tttcctttca aatgaaaacg atcgtcgtct
    25141 aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg cctttttcgg
    25201 caatctgccg ttttttggcg cttttgccct gctgactttt gaggaaatcc acatgtcatt
    25261 accacactgc ccaaaatgca actccgaata cacttacgaa gataacggca tgtacatctg
    25321 cccggaatgt gcctacgaat ggaacgacgc agaacctgca caggaaagcg acgagctgat
    25381 cgttaaagat gctaacggca atctgctggc tgacggcgac agcgttacca tcattaaaga
    25441 tctgaaggtg aaaggtagct cttcgatgct gaaaattggc accaaagtga aaaacatccg
    25501 cctggttgaa ggcgaccata acatcgattg caaaatcgac ggttttggtc cgatgaaact
    25561 gaaatctgag tttgtgaaaa agaactgatt gtattgtgat cggtaagccg gataaggcgc
    25621 tcgcgccgca tccggcaacg atgccgactg cctgatgcga cgcttgcgcg tcttatcagg
    25681 cagcctctca tctgtataaa tttcgaacta cacttaactg gcttctctta actgaggtca
    25741 ccatcatgcc gttaagtccc tacctctctt ttgccggtaa ctgttccgac gcgattgcct
    25801 attatcaacg tacgttgggc gcggaactgc tctataaaat cagcttcggc gaaatgccga
    25861 aatcagcgca ggacagcgcc gagaactgcc cttccggaat gcaatttccc gataccgcca
    25921 tcgctcatgc caacgtgcgc attgccggaa gcgacatcat gatgagcgat gctataccgt
    25981 caggaaaagc cagctactcc ggctttacgc tggtgctcga ttcgcaacag gtcgaagaag
    26041 gaaaacgctg gtttgataac cttgccgcta acggaaaaat cgaaatggcc tggcaggaaa
    26101 ctttctgggc gcatggcttt ggcaaagtca ccgataaatt tggcgtaccg tggatgatta
    26161 atgtcgtcaa acaacaacca acgcaataac ccgccgggag gcccgccctc ccgcactgtc
    26221 atcgaattcc cgttaactct tcatctgtta gtcactttta attaaccaaa tcgtcacaat
    26281 aatccgccac gatggagcca cttttttagg gaggctgcat catgcaaacg attatccgtg
    26341 tcgagaagct cgccaaaacc ttcaatcagc atcaagcgct gcatgcggtt gatctgaaca
    26401 ttcatcacag tgaaatggtg gctctgcttg ggccgtcggg ttccggcaaa tccacccttt
    26461 tacgtcattt aagcggtttg attaccggcg ataaatccgc cggtagccat atcgagctac
    26521 tgggccgcac tgtccagcgc gaaggccgcc tggcccgcga tatccgcaaa agccgcgcca
    26581 acaccggcta catctttcaa caattcaacc tggtgaaccg cctgagcgta ctggagaacg
    26641 tgctgattgg cgcgctcggc agcacgccgt tctggcgcac ctgttttagc tggttcaccg
    26701 gcgagcagaa acagcgcgcg ttacaggcgc tgacccgcgt tggcatggtg cattttgccc
    26761 atcagcgcgt ttccaccctc tccggcggcc agcagcaacg tgtggcgatt gcccgtgcgc
    26821 tgatgcagca ggcgaaagtg attctggccg atgaacccat cgcctcgctg gacccggaat
    26881 cagcgcgcat cgtgatggac accctgcgcg acatcaacca gaacgacggc atcaccgtgg
    26941 tcgtcacgct gcatcaggtg gattacgccc tgcgctactg cgaacgcatc gtcgccctgc
    27001 gtcaggggca cgtcttctac gacggcagca gccaactgtt tgataacgaa cgttttgacc
    27061 atctctaccg cagcattaac cgcatcgaag agaacgcgaa agctgcctga tatccccatc
    27121 attgaggaaa acgaatgaac gctaagataa ttgcctcgct ggccttcacc agcatgttca
    27181 gcctcagcac cctgttaacc ccggcacacg ccgaagagca ggaaaaggcg ctgaatttcg
    27241 gcattatttc aacggaatca cagcaaaacc tgaaaccgca atggacgccg tttttacagg
    27301 atatggagaa gaaactgggc gtgaaggtca acgccttctt tgccccggac tacgcgggca
    27361 ttatccaggg gatgcgcttc aataaagtgg atattgcctg gtacggcaac ctgtcggcga
    27421 tggaagcggt ggatcgtgcc aatggccagg tcttcgccca gacggtcgcg gcggatggat
    27481 cgccgggtta ctggagcgtg ttgatcgtca acaaagacag cccgatcaac aacctgaacg
    27541 atctgctggc gaagcggaaa gatctcacct tcggcaatgg cgatcctaac tccacctctg
    27601 gcttcctcgt ccccggttac tacgtcttcg ctaaaaacaa tatctccgcc agcgacttca
    27661 agcgcaccgt caacgccggg catgaaacca acgcgctggc cgtcgccaac aagcaagtgg
    27721 atgttgccac caacaacacc gaaaacctcg acaagctgaa aacctccgcg ccggagaagc
    27781 ttaaagagct gaaggtgatc tggaaatcgc cgctgatcct aggcgatccg atcgtctggc
    27841 gcaagaatct gtccgaaact accaaagaca agatctacga cttctttatg aactacggaa
    27901 aaacgccgga agagaaagcg gtgctggaac gcctgggctg ggcgccgttc cgcgcttcca
    27961 gcgacctgca actggtgccg attcgccagc tcgcactgtt taaagagata caaagcgtga
    28021 aaggcaataa agggctgaat gagcaggaca agctggcgaa aaccaccgag attcaggcgc
    28081 agctggatga cctggaccgc ctgaacaacg cgttaagcgc gatgagttcg gtgagtaaag
    28141 cggtgcagta aatcgtaggt cggataagac gccccggcgt cgcatccgac aatgtgtagg
    28201 cgttgatgcc ggatgcggtg caagcacctt atccggccta cagaccggag ccaaacatgc
    28261 aaaccatcac catcgcccca cccaagcgta gctggttctc gcttctgagc tgggccgttg
    28321 ttctcgacgt gctggtcgtc tcgtggcagg gcgcggaaat ggccccgctt acgctgatta
    28381 aagacggcgg caacatggca accttc
//
LOCUS       DAEEGA010000051        28338 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEEGA010000051 DAEEGA010000000
VERSION     DAEEGA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28338
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.051"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="KLH46_002368"
                     /pseudo
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="KLH46_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(998..2020)
                     /locus_tag="KLH46_002369"
     CDS             complement(998..2020)
                     /locus_tag="KLH46_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0379024.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="KLH46_002370"
     CDS             complement(2034..3536)
                     /locus_tag="KLH46_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBE0379025.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSMLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="KLH46_002371"
                     /pseudo
     CDS             complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="KLH46_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4942..5472
                     /gene="ppa"
                     /locus_tag="KLH46_002372"
     CDS             4942..5472
                     /gene="ppa"
                     /locus_tag="KLH46_002372"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBE0379026.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5851..6192)
                     /locus_tag="KLH46_002373"
     CDS             complement(5851..6192)
                     /locus_tag="KLH46_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBE0379027.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="KLH46_002374"
     CDS             complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="KLH46_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBE0379028.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="KLH46_002375"
     CDS             complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="KLH46_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBE0379029.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11910..12548
                     /gene="msrA"
                     /locus_tag="KLH46_002376"
     CDS             11910..12548
                     /gene="msrA"
                     /locus_tag="KLH46_002376"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBE0379030.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12871..14214
                     /locus_tag="KLH46_002377"
     CDS             12871..14214
                     /locus_tag="KLH46_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBE0379031.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14276..14482)
                     /locus_tag="KLH46_002378"
     CDS             complement(14276..14482)
                     /locus_tag="KLH46_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBE0379032.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14807..15361
                     /locus_tag="KLH46_002379"
     CDS             14807..15361
                     /locus_tag="KLH46_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBE0379033.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15424..16362)
                     /locus_tag="KLH46_002380"
     CDS             complement(15424..16362)
                     /locus_tag="KLH46_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379034.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="KLH46_002381"
     CDS             complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="KLH46_002381"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBE0379035.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17504..19447
                     /gene="cpdB"
                     /locus_tag="KLH46_002382"
     CDS             17504..19447
                     /gene="cpdB"
                     /locus_tag="KLH46_002382"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBE0379036.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFEISPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19573..19953)
                     /locus_tag="KLH46_002383"
     CDS             complement(19573..19953)
                     /locus_tag="KLH46_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBE0379037.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20042..20902
                     /gene="qorB"
                     /locus_tag="KLH46_002384"
     CDS             20042..20902
                     /gene="qorB"
                     /locus_tag="KLH46_002384"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBE0379038.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21011..21976
                     /locus_tag="KLH46_002385"
     CDS             21011..21976
                     /locus_tag="KLH46_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBE0379039.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATVQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22084..22746
                     /gene="ytfE"
                     /locus_tag="KLH46_002386"
     CDS             22084..22746
                     /gene="ytfE"
                     /locus_tag="KLH46_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBE0379040.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="KLH46_002387"
                     /gene_synonym="dagA"
     CDS             complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="KLH46_002387"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBE0379041.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="KLH46_002388"
     CDS             complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="KLH46_002388"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBE0379042.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25350..25895
                     /locus_tag="KLH46_002389"
     CDS             25350..25895
                     /locus_tag="KLH46_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HBE0379043.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25879..26526)
                     /locus_tag="KLH46_002390"
     CDS             complement(25879..26526)
                     /locus_tag="KLH46_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE0379044.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26738..27529
                     /locus_tag="KLH46_002391"
     CDS             26738..27529
                     /locus_tag="KLH46_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HBE0379045.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27539..>28338
                     /locus_tag="KLH46_002392"
     CDS             27539..>28338
                     /locus_tag="KLH46_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBE0379046.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6954 a         7539 c         6988 g         6857 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccact aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacaat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccatttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcatt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgctgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
     4141 ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac
     4201 ttcttttacc agccagtcac cgatcaactt accttcgagg atgttgtcgg cagtaacggt
     4261 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcgagcaaga agaccgggat
     4321 ttcggcatct ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat
     4381 aaagatcgca tccacccctt atgcaacgaa ggaacgtacc gctttaatct ggttttcctg
     4441 cttttgctga ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc
     4501 cacattggtt tctgcggcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt
     4561 taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa
     4621 gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg
     4681 aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta
     4741 aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac
     4801 ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga
     4861 cgaaaacaag caaagacatt cggcgcgagt tggctataat acttggcact tgtttgccac
     4921 atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg
     4981 gaagacatct acgttgttat tgagatcccg gctaacgcag atccgatcaa atacgaaatc
     5041 gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg
     5101 tgcaactacg gttacatcaa ccacaccctg tctctggacg gtgacccggt tgacgtactg
     5161 gtcccgactc cgtacccgct gcagcctggt tctgtgatcc gttgccgtcc ggttgccgtt
     5221 ctgaaaatga ccgacgaagc cggtgaagat gcgaaactgg ttgcggttcc gcacagcaag
     5281 ctgagcaaag aatacgatca cattaaagac gtaaacgatc tgcctgaact gctgaaagcg
     5341 caaatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggtgaaagtt
     5401 gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgca
     5461 aagaataaat aagttcttct agcgcaataa ccctgaacgc cgggcttcgg ttagtaaggg
     5521 tttttttatg cccgcgataa ataaactctc tattccacca tcattattct cagcggttgc
     5581 aaggcttgaa cggtaagaac aagcaaaccc gaccaccatt ttgctgttca tagccacttg
     5641 ctggaagtta gccgacctca ctcatactca ccgttcaaag cgtgcccccg ttttacccct
     5701 gcacatttgt gaacgccagc agagtttttt atgcgattgc agatatggaa agcggctcgg
     5761 agagaaaagc aaaagatgga aaagttacaa aaaaaatcgc gagatacatc gcataaaaca
     5821 acgccacccg aaggaggcgt gcttttatta ttacttatcc ctgtctaacc agtcgccgct
     5881 ttcaattagc tttaatccat cgacgggtcg ttgataaacg tacatccatg cactcccata
     5941 cggcgtctga atcaactggc gcgcgtattc accgcccctg gtgcgcaagg catcaagttc
     6001 ggccagcgtg gcgttgtcaa tacgataaac ttcaccgtgt accgttccgt tccccggaac
     6061 tgcgcctgga tagtggccca ggctatacaa ctggtagtta tcgatactga aatcgcccag
     6121 taactgggca ttggtcatcc agtgactgtt gccttgtttg tggcgtaaac tgccgtagac
     6181 aaatattcgc attgctaaaa ctcgaactga tagagcaaat ccagtgcctg gtctacacca
     6241 gacacggctt ccagatatag cttaggcatc aggcgataac gtaacgtgag tgttgctata
     6301 gagtcaaata tacccacgcc gtatttcact tgcagacctg gcaatacata gccgctgacc
     6361 actacctggg aggagtcgcc tactcccagg gtgtcgagcg ctaaattgct tacgccaaac
     6421 gtctcgccga ttttacccac aatctggcca ctttgcgcaa cccccagacc aatcagcatc
     6481 gaggtcattg ccgcactgtc gctctgatcg ctctccagcc cttgtccacg tagcaaataa
     6541 gacaatgcag cttgttgcga catcgctggg tcagagaaga tctccgcttt cggttcgtcc
     6601 gccagaccag tgacgcgaac tccggcgatt acatcgtctt ctgtagcatc cgggttacga
     6661 atagcttcaa tattaagata cggttgatcc ggcggaccag agaacagtaa ctcaccctta
     6721 cgcacaatca gatcctgacc ataggcatgg aagcgccctt cagggatgtt gatctgcccg
     6781 ttcaggccca gcccttgttt gtcctgtacg acgttaagat cgcccgtcag ccgcgctttc
     6841 aggccaaagg cgtcaatgcg cacattgttg ccaacgtgga caatcaggtt actgttaatc
     6901 ggaatcgacg ccgttttcgg ctcttccggt tgcaggttat cgttaagcat caccacatcg
     6961 ctggagacgc ctactgcgct ttccggcaga tcgtgcacca cgatgcgcgc ccacgggaca
     7021 tccacgcgac catcgagggt aaacaggttt ggtgtagcct cgaatacaac atctggcgat
     7081 acatccattc gtaccatcgg cggcacggtg atccgcactt tactgccttt cgccgttact
     7141 cgtgcccgcc agttttcaat ttggctccag tcggcattac cgttcaggta gatttcaccc
     7201 tgctgggtcc gtactgtacc ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc
     7261 tggctcggct gcatatcaaa cggcataaag ttgccgtcga tatccacacc cgtaacctga
     7321 agctgaccaa acaactgcgg gctttgcaca tcaccaccca gacgcaagtt ggcactcacc
     7381 atccccgctg ctttttcccc acgggtaaag atggggttta tcatcgcaag gttgaagtta
     7441 cggatattga cgttgccacc aagattacgg cggccttgcg gatcggtcac ctgcacctgt
     7501 ccatcaaact ggccgttatt ggtcaggcgg atggtccagc ccaattcggc acggttgtta
     7561 cgcaattccg ccgtcagatt cagtgtctga aacgccaccg gcagcgccgc atcgttgacg
     7621 gtttgcgtta cctgcacgtt acgccccgaa agggtgatac tgccctgcgg cagcccctct
     7681 ttcgtggtgt cccaggcaac atccgcttta cccgtgaaga taccgctggc ctgagtggtt
     7741 tctggcataa acggtttcag catggcgagg tcgaagcggt tgagattcac caccgcacgc
     7801 ccttcggccc ccgcatcgat agtttgcggc acgcacagtt ccgcattcgg gttaagccag
     7861 cagtgtggcc cgatgctgat tttttgctcc ttattgcggt aatccagcgc aatatcgcgg
     7921 gtcagcgacc acgggccgac cggcgtctgg aagcgggtat tgctaagagt tcccttccag
     7981 cgttcttctt tgcgatcaaa acttcctgcc agattaagct gcccggagac aggctcgccc
     8041 tgaatccgca actgtagctc gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg
     8101 ttgatattta catccggctg cgaaatttgc tcaacgcgta cgtcgagttt cccggcgatc
     8161 tgatcggtgg atttaatgtc gccttccacg cgaacctgcg ccacggaaag ttcctgccag
     8221 cgcaggccgc gcgcggtgat atctgccagt agttgtggcg cttccaccgt gccgcgtact
     8281 ttcaccagcc ctttcgctgt accgccaagc cccggcagcg cgttatccag ccccggcgcg
     8341 ttgatggtgg catcaagatt gagatctttt acccccagct cgcctttcac ttcggcactg
     8401 tttggcccga gttccagatg aagcccaggg atcatccact gcatataact gttgcctttc
     8461 agcgtgccgt caacgttcac tttgttctgt ttgacgttac cggtcagctt cagttctggc
     8521 acctccatct gccaggtgcc gccgtacagg ctaccgcggg ttttaatcaa gccattgagt
     8581 ttcgacggcc actccgggaa ctctttggcg gtgttaatgc cattaagcgt tagctcaccg
     8641 cgccaactaa tggcctgctg ccagtcgagc aacgccttga gttcagtttt cccttccagc
     8701 gccgcgacgg tgagtttgtc gagattgacc tgctgttcat tacctttggc atcaagggta
     8761 atggtggctg gcgggatctc ctgtcccttc actgccgtac gcatagagag cgtgtaatcg
     8821 gtcattttgc cggtaagttt cagtttcaga tcatccgcct gatactgctt ctcaccagtg
     8881 aacggccagt aaagctgttt gctgttcact tccacgttga gtggcaatcc ggcttccgcc
     8941 agtcgcgtct gggcgcgtaa atccatatcc accggaccgg aaaggttaac gcctatctcc
     9001 agctgttcgc gcagcgcgcc gcccactttc agcttcacct tttcaccttt caacggctcc
     9061 acgttcagtg tgctgttgag ggtgatatcc accggccagt tgtctgacag ctgcgccgtg
     9121 ccgctggcgt tgacgatccc ctggttggaa tcgatatcca gggcgtccag tttagtattg
     9181 ccgtcaatgc tgctcacttt cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc
     9241 acgcgcagct gctcgccctt aaactcttca atgttcaggt taagcggcaa atgcacgtcg
     9301 gtcatttccg gcaataccgg gcgagaaaag agatctttca gcgtttcgcc gagcggcttt
     9361 tcctccggct gcggattttc aattttcggt tcgaccactt cttcctgcgc cacttccacc
     9421 actttcggca gagcaatcag caggcctttc agcgacgtcg gtttcagggt cagggttttc
     9481 tcctgccagt tcaggccgga ggtgaagtcc atcaccgaca ccgtggtgtc atcaatcttg
     9541 atgttgacgt tgtccagcgc cacccgtgtc agggtgatgg gatacggcgt ggagagatcc
     9601 agcggaccgc tatcttcctc ttcttcaacc tgttcagaag gaggcatttt tttactgtcg
     9661 atgttgacct gaatgtcttt cagcgccagg tcattaatac aaacgctact gttccacagg
     9721 cactcaagac cgacccccag atgcagatta cccgctttta ccgccacgcc tagctgctca
     9781 taacgaacgt cagacaaggt gagatcgcgc cagccgccgg tgaccttgcc aatatccagt
     9841 cctggcaccc agcgatccgc cgctttaaat acaagatgca ggccgctggt ggtgcccacc
     9901 agaaacgcca ccgatcccag caacagtaag ataacgataa ccacgccgag gctgattttt
     9961 ttccataaac tcataattct ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt
    10021 tatccgcgac cggtacggca aaatcgagtt tgattggccc gaccggcgat tcccagcgta
    10081 cgccgacccc ggtaccggtt ttaaagtcgc tgcggcgaat atcgcttacc gcttcgccac
    10141 tatcgacaaa caccgcgccc caccattttc cggtcacgtt gtactggtat tccagcgatc
    10201 cggttatcaa cttcgaggcc cctttcaggt caccgttggc gtatttcggg gcgatagatt
    10261 tgtatttgta gccgcgaata ctgcgatcgc ccccgacgaa gaaacgcaga tccggcggta
    10321 ctttgtcgaa atcaccggtt tcaatccagc ccagcgtgcc gcgtgtcaca aagcgatggc
    10381 gatcgtacag tgtgcggatc cagacgtgct gcgcctggaa aacggagaaa tcgacatctg
    10441 aaccccaggc cgtgttggag tagtcgatag agtagcgttg cgagtctccc caggttggca
    10501 tcaggccacc acgagaacgc gtgcggctaa tcatcacccc aggataaaac agcatcgtgg
    10561 tgttggtaat ttcaccctgg gtaaagtggt cgaggctcca gcgcaggtta atggcacgct
    10621 gccagccgct ggagagatcc cagtagcgag aagccaccaa cgtagtggag tcagattcgg
    10681 tatcgttcag gtcagtgcgc ttaaaaccgc cctgcaccaa ataatattgt tccagtggat
    10741 tcttcagcag cggcatttta tagctgaagt cgagggtctg ttccggcgcg gaaatactgg
    10801 tactggtggt caaactgtga ccgtatgagt tcatccacgg ctttttccac gtcgctttca
    10861 cgcgcggtcc cacgtccgta gagtaaccga ccccggtttc gatggtgttt tctgttcgcg
    10921 gcgaaaccac gcccgtcaat ggtaatactt tcgtttcgcg cgctttatca aattgtggag
    10981 ccaccaccac cgagttaaac cagccggtag cagaaagtcg gcggttcagt tccgccagat
    11041 ctttcgattc atactcatcg ccctctttaa acggcaccag attttgcagg tattcatcgc
    11101 ggatttgtga tccttcaaag gtcacatgcc caaagcggta acgttcgcca ctgttataat
    11161 caatatccca gaaggcttta tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt
    11221 cgctatcgaa ataaccttta cgcaacgcaa tactggttaa ggactttttg aaattttcat
    11281 aatcgccctg gttcagtacc gtaccaatag ccgggcgagt atcgagcaat ttcaaatagt
    11341 ctttatcggt ccgcgcgccg ccgcgcaata ccacatcggt gccgccaatt aacaccggca
    11401 cacctggcgt gactttggcg atcaatacct gccgcccttt ctttggcggt ggacggagat
    11461 caaattcaat ggtcggctgg taataaccca gcgctttcag accttcacgg atggcatcat
    11521 cgacgcgtgc gcgaaagcga cggtctggcg tcacttcatc actttcaatc gtagaaagct
    11581 gcgcacgaac gttcttttcc agctgtcccg ataacccctc gacctgtaga cggacgttcg
    11641 cggcgacggc agatccgctt aagcagagta agcttacaca gcataactgt cggatatagc
    11701 gcacattttc tcctgaatat ccttttttcc tgcccctgga acgccgttaa acggcttaac
    11761 aaaaatccag taatatggat taaaaagcag actaaacccc aaatatttct tatgttttac
    11821 tttagaccta ttcacggtgg ttattgtgtg caaatacgcc tcttgttaca accttgaccc
    11881 caatgaccga ttttcgggag agcgacacca tgagtttatt tgataaaaag catctggttt
    11941 cccccgccga tgccctgcct ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg
    12001 tcaacggtca ctcaatgacc aatgtacctg acggaatgga gattgccatt tttgcgatgg
    12061 gttgtttctg gggtgtggag cgtctgttct ggcagttacc cggcgtttac agcaccgccg
    12121 caggctatac cggcggctat acgccaaatc cgacttatcg ggaagtgtgc tccggtgata
    12181 cgggtcatgc cgaagcggta cgcattgttt acgatccttc cgtcatcagc tatgagcagt
    12241 tgctacaggt attttgggag aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc
    12301 acggcacgca gtatcgttca gcgatttatc cgctgacccc agaacaggat gccgcagctc
    12361 gcgccagtct ggaacgtttt caggcggcga tgcttgccgc cgatgacgat cgtcacatca
    12421 ccacggaaat cgctaacgcc acaccgtttt attatgccga agatgaccac cagcaatatc
    12481 tgcataaaaa cccatatggt tactgtggaa ttggcggaat tggcgtctgt ctgccgccgg
    12541 aagcatagcg ttacgggtac aaatgtagtt tgttgataaa gtgctctttg tctatgcctg
    12601 atgcggctta aacgacttac taggcctaca aaatcgccca aattcaatat attgcaagga
    12661 ctgcgtaggc ctgatcggca tagcgcatca ggcagtattg catttatcag cagtctgaat
    12721 tttaaccctc tggcgacttt acagtacctt acgctatact agccactgaa aatgccggtt
    12781 cactttcttc gaatcggctt tcaatgtgta tttcacacaa attaatcaac tttcccttcc
    12841 gagggtctgg cctgaaaggt cggataagat atgttaaaca gtattttagt catactctgc
    12901 ttgatcgctg taagtgcgtt cttctcgatg tccgagatct cgcttgccgc ctcacgcaaa
    12961 atcaaactta aactgctggc tgatgaaggc aatataaatg cccaacgcgt tctgaatatg
    13021 caggaaaatc ccggcatgtt ctttaccgtg gtccaaatcg gtctgaacgc agtggcgatt
    13081 ctcggcggta tcgtcggtga tgcggcattt tctccagctt ttcacagcct gttctcccgc
    13141 tatatgtcgg cagagctctc tgagcaactg agctttattc tctctttctc gttagtgact
    13201 ggcatgttta tcctgtttgc ggatttaacc ccgaaacgca tcggtatgat tgcgccagaa
    13261 gcggtggctt tgcgtatcat caacccgatg cgcttctgcc tgtacgtttg caccccgctg
    13321 gtgtggttct tcaacggcct ggcgaacatg atcttccgta ttttcaaact gccaatggta
    13381 cgtaaagatg acatcacttc tgatgacatc tacgcggtag tggaagccgg tgcgctggcg
    13441 ggcgtgttac gtaaacagga acacgagctg attgaaaacg tctttgagct ggaatcccgt
    13501 accgttccgt cttcaatgac accgcgtgaa aacgtgattt ggtttgatct ccacgaagat
    13561 gagcaaagcc tgaagaataa ggtggcggaa catccgcact ctaagttcct cgtctgtaat
    13621 gaagatattg accacatcat cggttatgtc gattctaaag acctgctgaa ccgcgtgctg
    13681 gctaaccaaa gcctggcact gaacagcggc gtacaaattc gcaacacgct gattgtgccg
    13741 gatacgttaa ccctttcaga ggcgttggaa agttttaaaa ccgcaggtga agacttcgcg
    13801 gtgatcatga acgagtacgc gctggtggtg gggatcatca ccctcaacga cgtgatgacc
    13861 acgctgatgg gcgatctggt cggtcagggg ctggaagagc agattgtcgc ccgtgatgag
    13921 aactcatggc tgattgacgg cggcacccca attgacgacg tcatgcgcgt gctggatatt
    13981 gacgagttcc cgcagtcggg caactacgaa accatcggtg gctttatgat gtttatgctg
    14041 cgtaagatcc cgaaacgcac cgattcggtg aaattcgccg gctacaaatt tgaagtggtg
    14101 gatatcgata actaccgcat cgaccagctg ctggtgaccc ggatcgacag caaggccacc
    14161 gccctttcgc caaaactgcc tgacgctaaa gataaagaag aaagcgtcgc gtaaccccca
    14221 gaaacatcaa cggctcctga atcaggagcc gtcttattac tgcatagcac tttggttaag
    14281 ccatctctgt ttgcagacgc ataacctgac ggttaacttc ggacatcact gacaaatgca
    14341 gcttatcctt cacttttggg ataagaatct tacccttatc aaattcaaaa gcgccaatgt
    14401 ccttgatgta taaccgtcca cggaacagga tctttacgta cttcgccacc tgaagtgggt
    14461 tgtaccgttg gaaaattttc attgttatct cctgctgagt attacgcctg tgcggtgcca
    14521 caatcggccc aactattatg aggcgcaaat tttaatgctt agtgactata gactatccgg
    14581 gcaatgtttc caccgcgtat aacttttttt acctaaaggt tacaattatt cagaattatc
    14641 tttttaccga agcgcggtct tcagtataag cattcatttt tcatatgatt tgtgcgcttg
    14701 accgcaaact ggcatcacac ttgcgggaaa ttcgataaat agcacatatg attaaaactc
    14761 agacccaagt ggtcggatca cctgcatatc ataagaagga aacaccatga ccctacgcaa
    14821 gattctggca ctcacctgcc tgctgttgcc gatgatggct tccgcacatc agttcgaaat
    14881 cggtcagcga gtgccgccga ttggcattac cgatcggggc gagttggtgc ttgataaaga
    14941 tcagtttagc tacaaaacct ggaacagcgc gcagttagtg ggaaaagtgc gagtactgca
    15001 acatattgct ggtcgcacct ctgcaaaaga gaaaaacgcg acgctgattg aagcgattaa
    15061 atcagcgaag ttaccgcacg atcgttacca gaccaccacc attgttaaca ccgacgacgc
    15121 gattccgggt tccggcatgt ttgtgcgcag cagtctggag agtaataaaa agctttatcc
    15181 ctggtcgcag tttattgtcg atagcaatgg cgtcgcgcgc ggtgcctggc agctggatga
    15241 agaaagttcc gctgtagtgg tgctggataa agacggtcgc gtgcaatggg ccaaagacgg
    15301 ggcgctcaca ccggaagagg tgcagcaagt gatggacctg ctgcaaaagt tgctcaaata
    15361 atggcaataa cataaattct ggaaaaataa ttttccccta tcgttatcta tgggataggg
    15421 gaattaagtc tctgagttct tacaccgaac taatactggt aacgctagtt gctctttttt
    15481 aaacgcctca atggtcgatt tatatttctc gcaaatcttt tctttggtaa tattactatc
    15541 tgcggccaga ttcgcactta ctatcgcatc cacttgttcg gctaattcga cttgttggca
    15601 acctgcatta accagaaaat tataaataag agagtaatct tcatcctcca aaacaacaga
    15661 ggaaccatta cgaaaacgtt catcccctcc atagatttta agcgcaaagc ccaaagattc
    15721 tatgactatg gcaaaattat catcaattat tttttcgtaa atataccatc catcgcattc
    15781 tttaattcca ccgcctggta tttggagttc aaatttaccc cgttttaatt tgcttactac
    15841 atcctccaat aaggcataac cgaaagatac gtcaccaaac gcctggcgaa tataaccttt
    15901 aatccttttt ggtatgtcag actgtttttt ctgcaaaata tcacaggcat acaatcttct
    15961 gttttgttct aacggaaact cgcggttttt gaaatccatg aatgagttat actcatttat
    16021 tgttatggga atttcttcgt attcaaaccc actgcctcgc aatgttattg tatattcagc
    16081 accgttatct ttaaggctga aactgataag aaaattatcc atggcattaa cagtttcatt
    16141 ttttgatgaa agcacttgat aactattttt atcaatctgt gttgcgcaac gaaaggactc
    16201 ccgcaaagtt aacagactat cataattcga aacccaatct tttttcttaa atgcatgctg
    16261 aatattatta aaaaaatcgt ttgaatgtgt ttttgtatgc gatgacttta caaataactc
    16321 caccgcacca ataaaattat tgtgtctgat cctgggtaac atatggctac cttattttat
    16381 ttggttattt tatgtcacca ttaaatatct cattatagaa gtaaactatc aaaaaacgct
    16441 cattattaaa atgtaactta ctagcaattg aatttttgca ctttaaatac aatctctttt
    16501 tatttataac tttttaggtt gggataaatc aggatggcag aatcaggaaa tacactgttt
    16561 ctgccatctg aatttagtaa atggacactc tgaaccccgg attcaggaac gactcgcgcg
    16621 gagtgtaatc cagcggttta ccctgccagt cgtgaacgtg cgctccggca gctgcagcca
    16681 cagcatgtcc ggcggcggtg tcccaaatat tcgttggtcc gaagcgcggg tacagctgcg
    16741 cctgtccttc cgccaccagg cagaatttca gcgaagagcc gatggacgtg gtctgatgtt
    16801 cgccaagctg ttgcagatac tctttcagct ccgcatctgc atgggaacgg ctgataacca
    16861 ccagcggcgg gcgcgcatcg cggacctgaa tcagcttgcg cacaccgcac tcttctttcc
    16921 aggccttgcc ttctgccgcg ctgtacatta cgttcattac cggcgcatac accacaccta
    16981 aaatcggttt gccatggtca atgagcgcaa tgttaacggt gaattcgcca ttacgtttaa
    17041 taaactcttt agtaccatcc agcgggtcta ccagccagta acgctgccag tgctgacgga
    17101 cttcccaacc gggaggatct tcttcagaaa ggaccggaat atccggtgcc agcgtacgta
    17161 aaccgtccat gataacggtg tgagcggcaa tatccgctgc cgttaccgga gaattgtccg
    17221 ctttgctgac gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg gcatcgcctg
    17281 cattccgtgc aagctggcat acttgatcta acatttctcc acctcgtctc tgtgagcggt
    17341 gttaacttat tgttttactt ataccctatc gttaatgaat gcgccaactg tgatagtgtc
    17401 atcattttca aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa ggcgtttatt
    17461 ctcccttttc tttcgtattc ccgatgataa aaggatgtcc ctgatgatta agtttagcgc
    17521 aacgctcctg gccacgctga ttgccgccag tgtgaatgca gcgacggtcg atctgcgtat
    17581 catggaaacc actgatctgc atagcaacat gatggatttc gattattaca aagacaccgc
    17641 cacggaaaaa ttcggactgg tacgtacggc aagcctgatt aacgatgccc gcaatgaagt
    17701 gaaaaacagc gtactggtcg ataacggcga tttgattcag gggagtccgc tggccgatta
    17761 catgtcggcg aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac
    17821 gctggattat acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct
    17881 gaaaaatgcg ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag
    17941 aaccaaacag ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga
    18001 cggaaaaaaa cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg
    18061 ctgggataaa gctaatttat ccggaaaagt gacggtgaat gatattaccg aaaccgtgcg
    18121 caaatacgtg cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc
    18181 cgggctgtct gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga
    18241 aattccgggc gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga
    18301 ttttgctgat atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt
    18361 aatgccgggc atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag
    18421 cggtaaatgg caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa
    18481 taaaaaatcc ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga
    18541 tgccacacgc cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta
    18601 tctggcgctg gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta
    18661 tgtcgaacat tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc
    18721 cgcaccgttt aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa
    18781 aggccagttg accttccgta atgccgccga tctttatctc taccccaata cgctgattgt
    18841 agtgaaagcc agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa
    18901 ccagattgct cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac
    18961 ttataacttt gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg
    19021 ctatgacggc gagtgccaga tgattaatgc caatgcagag cggattaaga acctgacctt
    19081 taatggcaag ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc
    19141 ttacggcggc aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga
    19201 gaaccgctcg gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca
    19261 tccggcggca gataacaact ggcgtttagc accgatagct gccgataaga aactggatat
    19321 ccgtttcgaa atttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta
    19381 tccgatgaat aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag
    19441 taagtaaaac acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat
    19501 gcgatgctgc cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag
    19561 cgcatccggc agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa
    19621 ctcaatccag tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag
    19681 gctatattcc acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg
    19741 ttctaacgcc tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg
    19801 caggtcgcta aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg
    19861 gctggtgacg tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc
    19921 tttcagttgt tgtgacaggc taacctgact catttcatac ttaccttttt gcacgtactt
    19981 actaaaagta agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc
    20041 catgatcgct attactggtg ccactggcca acttggtcac tatattattg aatccttgat
    20101 gaaaacggtt cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc
    20161 cctggcagca caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac
    20221 atctgcactt cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg
    20281 tgccccgcag catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc
    20341 ttataccagc ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga
    20401 gacggagaaa atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta
    20461 cagcgaaaac tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc
    20521 ggcgggcgat ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcacg
    20581 cgtgattagc gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg
    20641 gacgttgaca cagttagcgg cagagctgac caaacagagc ggcaaacagg ttacctatca
    20701 aaatctgagc gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact
    20761 ggcggatatg ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag
    20821 caaaacgctt agcaaattga ttggctgccc aacgacaacg ttagccgaaa gcgtaagcca
    20881 tctttttaat gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa
    20941 tgacgggatg ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg
    21001 gatgccagta atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct
    21061 tatttttgtc gggccgttga tcgtgccgga atacccggcg atgttgcagt cgatggggcg
    21121 ttatctggcg ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg
    21181 tcagttggcg cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat
    21241 ttattacttc tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat
    21301 tatcggcacc ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga
    21361 cggcaaactc gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc
    21421 gagtgtgaat attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac
    21481 ctctggcatc gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa
    21541 cgcccgctgg ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggtgcaggc
    21601 gctggtcacg ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat
    21661 acaaacgccg gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat
    21721 ggttgcgata gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca
    21781 gcgattaccg acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct
    21841 gtacaccttt ttactccgcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct
    21901 gttagtgatt ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc
    21961 tgtctcagaa agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta
    22021 tagcttttgc cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca
    22081 gctatggctt atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg
    22141 gctctgtttc gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc
    22201 gcggcggcac gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct
    22261 gaacaaccga ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc
    22321 atcgtgcgct accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact
    22381 aaagtcgagc gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg
    22441 accatgctgc atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg
    22501 atgatcaaac aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag
    22561 cacgatgaag cgggcgaact gctggaagtg atcaaacaca ctaccaataa cgtcacaccg
    22621 ccgccagaag cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat
    22681 gacctgatgg atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt
    22741 gagtgatcta aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc
    22801 agttcagcag cccgcttctt accaataaac agccagccca accccagcgc gataaaccac
    22861 agcggggtaa ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg
    22921 aacacaaaga acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc
    22981 gacttttcat gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc
    23041 gtccagacga acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc
    23101 acgctaggat tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc
    23161 aaccctttcg cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct
    23221 tcctgcgcca gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac
    23281 gccgcagagg tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac
    23341 acaaacagct caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac
    23401 ataatcacaa tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc
    23461 gcgcgtggca gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca
    23521 atccccacga aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc
    23581 gggaaccagc cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac
    23641 tgaaagtgca tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt
    23701 ttgatcatcg caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc
    23761 gtcagcagca gcactatcac cgccagcgag gcgacccagt cggagagatc ggggaaccag
    23821 aactgagcat aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac
    23881 cagtaagtcc agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca
    23941 ctaaaagatt tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa
    24001 aagagcataa aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc
    24061 gttttgccgg accccataaa caacccagta ccaatggcac ctccaatggc aataagctga
    24121 acatgtcggt ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca
    24181 acgactttta cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct
    24241 ctacggcctt tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg
    24301 taacaatacg cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg
    24361 agtgtcatat cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt
    24421 tgcaacacaa aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc
    24481 ctctggttcg aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa
    24541 caccagggtg ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc
    24601 ctgcgggata gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca
    24661 gccagggatc acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc
    24721 aaacacggtg ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc
    24781 cggaattgcg ccttcaccct ggttgatcac gcggaattgc aggccagatt cggtgctatt
    24841 cacaccttct tttttggcgt tttcttccag gtatttcacg ccttcagcag ccatcgcctg
    24901 gaaacgctga cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac
    24961 cacatcaacc ggcacagccg gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag
    25021 tgcttctggc agcagccctt ccaggccaga ttcactcagt tgttgcccta cctgcaaacc
    25081 aatgccgtag cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc
    25141 tttcttaggg tataaaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt
    25201 gtcgcaggaa tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat
    25261 ataataaagg cgggtttacc acggacatca tagcgttagc ttattcatag gctatgattg
    25321 aggaacaaga cgtggagcag gaggaaacca tgcccgggcg ctttgaacta aaaccaaccc
    25381 tggagaaagt ctggcacgcg ccggacaatt ttcgcttcat ggacccgctg ccgccgatgc
    25441 atcgtcgggg gatcattatt gccgccattg tattggtggt cggatttctg cttccatctg
    25501 atgatacgcc caacgcgtcg gtggtgacgc gtgaagcgca gttagatatt cagtcgcaat
    25561 cacaaccgcc gacagaagaa cagccgcaaa ctacccaaac gcaaccattc cagccagata
    25621 gcggaattga taatcaatgg cgctcttacc gtgtcgaacc cggtaaaacg atggcgcagc
    25681 tgttccgcga ccacggactg cccgctaccg atgtctatgc aatggcgcag gtagaaggcg
    25741 cgggtaagcc gctgagtaat ttgcaaaatg ggcagatggt gaaaatccgc cagaacgcca
    25801 gcggcgtggt gacgggctta accattgata cggggaataa tcaacaggtc ctgtttaccc
    25861 gccagccgga tggcagtttt attcgtgcgc ggtagatgaa gggtcaacca aaagccagct
    25921 aaggattgtc tctttgatga ccttacgtct ggcttccagt gcctgggggg aaaccatact
    25981 gatatgataa actaggccaa gcgtatgctg gttgatcaaa taatatcccc caagcgcagc
    26041 aatattaata ttcacctgca atgggtcgat acctggctta aaaatattgt atttcttccc
    26101 ttcatccaat aaggattcca ttaactgcaa gtgagcatgg ttaatttcta agagacgctg
    26161 tgattttgca taatgcacac ctttactttg attttcacta tgcacaattt ttaaaaacca
    26221 aggattagca atataataat cccatgtaaa atcaataatt ttctcaatgg ctttttgtgg
    26281 cggaagatct gagaaatcaa gggccttttc tttttgtcga atatcgttcc aggtatattc
    26341 cagcacttcg ataaataaat tttctttatt accaaagtgg tgataaaccg tctgtttact
    26401 acaccctgct gcctttacaa tattatctac tctggcccct tcataaccat attcggcaaa
    26461 ttcattaacc gcacatagca gcaatttttc cttcaggctc tgtacgttgc tggtatcaat
    26521 atacataacg cttccccacg acctcttcgt ggtgactatc ataacctgat ttaatttcag
    26581 cttcagaaaa aagggcaaaa acttgtactt tcatcaggtt atcaaatgtc ctgtgatcgc
    26641 catcgcatat caaccaacta gatggttgat ctttttatcc tccagtcgca agctaatcat
    26701 caccaaggaa acaaaattca aaaaagagag ctattgtatg aaatccactc gtcctgttgc
    26761 cgtgattacc ggagccactc gcggtattgg aaagggctgt gcgcaggagc ttgctcgtgg
    26821 cgggtttaac ctgctgatta acgatcgtcc cgatgccgac agcgtagaaa aactgcatat
    26881 cacacaacaa gaatgtttgg cggaaggcgt ggaagtgatt tgcttccctg ctgatgtcgg
    26941 tgatctatct ctgcatgagg agatgctcga cgcggcgcaa aaccagtggg ggcggctgga
    27001 ctgtctgctc aataacgcgg ggatttcagt gaaaaaacgc ggtgacctcc tcgacctcga
    27061 accagacagc ttcgatcaaa acattgccat taacacccgc gcgccgttct tccttgctca
    27121 ggcattcagt aagcgcttac tggcacagcc gaaaccggaa gcggagctgc cgcaccgctc
    27181 gattattttc gtcagttcca tcaacgccat catgctggcg atgaatcgcg gcgaatacac
    27241 cattgccaaa accgccgttt ctgccgctgc tcgtctgttt gctgctcgcc tttgtaatga
    27301 acaaattggc gtttatgaag tgcgccctgg tctcatcaaa actgatatga ctattccggc
    27361 aacggcttat tacgacgaac tgattgccaa aggtctggtg ccgtggggac gttggggtta
    27421 tccggctgat attgcttcca ccgtccgcgc gatggcggaa ggtaaactga tttacacctg
    27481 tggtcaggcg gtcgcaatcg acggcggctt aagtatgccg cgcttctgag gctgaatcat
    27541 gagagatctg ttacaacgcc cggatttatt cagtatcaat accgcgacac tgggctataa
    27601 aacgccgcta cccgccatta ttgatgcctg cgccgcacgc ggtattggcg caattgcccc
    27661 ctggcgcagg gagttgcagg gtgaagatct ccagcaaatc acccgccagc ttgccgccag
    27721 taacatgagc gtttccggtt tgtgccgtag cacttactac accgcgccga cactggcgga
    27781 acgcaaactg gcgattgatg ataaccgccg ggcgctggac gacgcagcgg tgcttaacgc
    27841 cgcctgttat atgcaggtgg ttggcggtct gccaacgggc actaaagatc tatatgaagc
    27901 gcgagaacaa gtgaaacaag ggattcgcca gttgctgcca cattcgaaag atgtcggcgt
    27961 gccaattgcg ctggagccac tgcacccgat gacagctgcc gatcgctctt gcctgtgcac
    28021 gttacgtcag gcgctggact ggtgtgatga actcgatccc gatggtgagt ttggcctcgg
    28081 cgtggcggtg gatgtttatc acgtctggtg ggatccggat ctcgccagtc agatcctgcg
    28141 tgccggaaaa cgcatcctcg cgtttcatgt ttccgactgg ttaatgccga ccactgatct
    28201 ggtcaatgac cgtggtatgc cgggagatgg cgtcatcaat attccgtcaa ttcgtcggct
    28261 ggttgaaaac gccggattta atggtgctat tgaactggaa atattctcgc cttactggtg
    28321 gcagaaagat attaacag
//
LOCUS       DAEEGA010000052        28288 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEEGA010000052 DAEEGA010000000
VERSION     DAEEGA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28288
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.052"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            564..1334
                     /locus_tag="KLH46_002393"
     CDS             564..1334
                     /locus_tag="KLH46_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBE0379047.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="KLH46_002394"
     CDS             1325..1798
                     /locus_tag="KLH46_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBE0379048.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPRLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="KLH46_002395"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="KLH46_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBE0379049.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="KLH46_002396"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="KLH46_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBE0379050.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="KLH46_002397"
     CDS             3233..3727
                     /locus_tag="KLH46_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBE0379051.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="KLH46_002398"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="KLH46_002398"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBE0379052.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="KLH46_002399"
                     /pseudo
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="KLH46_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7526..8335
                     /locus_tag="KLH46_002400"
                     /pseudo
     CDS             7526..8335
                     /locus_tag="KLH46_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            8550..9911
                     /locus_tag="KLH46_002401"
     CDS             8550..9911
                     /locus_tag="KLH46_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0379053.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLSQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9960..11624)
                     /gene="tsr"
                     /locus_tag="KLH46_002402"
     CDS             complement(9960..11624)
                     /gene="tsr"
                     /locus_tag="KLH46_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBE0379054.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11743..12189)
                     /gene="hpaR"
                     /locus_tag="KLH46_002403"
     CDS             complement(11743..12189)
                     /gene="hpaR"
                     /locus_tag="KLH46_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBE0379055.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12461..13750
                     /locus_tag="KLH46_002404"
     CDS             12461..13750
                     /locus_tag="KLH46_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBE0379056.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPAPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13747..15213
                     /gene="hpaE"
                     /locus_tag="KLH46_002405"
     CDS             13747..15213
                     /gene="hpaE"
                     /locus_tag="KLH46_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBE0379057.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVSEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15215..16066
                     /gene="hpaD"
                     /locus_tag="KLH46_002406"
     CDS             15215..16066
                     /gene="hpaD"
                     /locus_tag="KLH46_002406"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBE0379058.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16076..16456
                     /locus_tag="KLH46_002407"
     CDS             16076..16456
                     /locus_tag="KLH46_002407"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBE0379059.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16524..17327
                     /gene="hpaH"
                     /locus_tag="KLH46_002408"
     CDS             16524..17327
                     /gene="hpaH"
                     /locus_tag="KLH46_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBE0379060.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALMDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17338..18141
                     /gene="hpaI"
                     /locus_tag="KLH46_002409"
     CDS             17338..18141
                     /gene="hpaI"
                     /locus_tag="KLH46_002409"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBE0379061.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPASIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18163..19518
                     /gene="hpaX"
                     /locus_tag="KLH46_002410"
     CDS             18163..19518
                     /gene="hpaX"
                     /locus_tag="KLH46_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBE0379062.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19528..20418
                     /gene="hpaA"
                     /locus_tag="KLH46_002411"
     CDS             19528..20418
                     /gene="hpaA"
                     /locus_tag="KLH46_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBE0379063.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDEKYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCPPTQFRGR"
     gene            20445..21382
                     /locus_tag="KLH46_002412"
                     /pseudo
     CDS             20445..21382
                     /locus_tag="KLH46_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21609..23167
                     /gene="hpaB"
                     /locus_tag="KLH46_002413"
                     /pseudo
     CDS             21609..23167
                     /gene="hpaB"
                     /locus_tag="KLH46_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23185..23697
                     /locus_tag="KLH46_002414"
     CDS             23185..23697
                     /locus_tag="KLH46_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBE0379064.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24090..26240
                     /gene="btsT"
                     /locus_tag="KLH46_002415"
     CDS             24090..26240
                     /gene="btsT"
                     /locus_tag="KLH46_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBE0379065.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26290..26493
                     /locus_tag="KLH46_002416"
     CDS             26290..26493
                     /locus_tag="KLH46_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBE0379066.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26504..27460
                     /gene="yjiA"
                     /locus_tag="KLH46_002417"
     CDS             26504..27460
                     /gene="yjiA"
                     /locus_tag="KLH46_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBE0379067.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27529..>28288)
                     /locus_tag="KLH46_002418"
     CDS             complement(27529..>28288)
                     /locus_tag="KLH46_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBE0379068.1"
                     /translation="NYTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMART
                     EDFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQ
                     HADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQ
                     ALVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSF
                     YLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6919 a         7401 c         7092 g         6876 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctagattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgaccaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcact cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgccca ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc taacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cctaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tatccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gtacaacatc agccgcacta cagtgcgtca
     7561 tattctcagc caattacgcg aatgcggcgt cttaacgcag gttggaaacg actatgttat
     7621 tgcgcgtaaa cctgaccatg atgacggttt cgcctgtact accgcttcaa tgagtgaaca
     7681 aaacaaagtg tttgagcagg cttttttcac gatgataaac cagcgtcagt tacgccctag
     7741 ggaaactttt tctgaactgc aactggctcg ggccgcgggt gtcagcccgg tggttgtaag
     7801 agaatatctt ttaaaattcg gccgttataa tcttattcaa agcgaaaagc gcggccagtg
     7861 gagcatgaag caattcgatc aatcctacgc cgagcagctg tttgagctac gcgaaatgct
     7921 cgaaacacat tcacttcagc actttctcaa cctgcctgat catgatcccc gctggctgca
     7981 ggcgaaaacc atgctagaac gtcatcggct actgcgtgac aacatcggca atagctttcg
     8041 catgttctcg caacttgaca gagattttca ctccttactg ctttcagctg ccgacaatat
     8101 cttttttgat caatcgcttg agatcatctc agtcatcttt cattttcact atcaatggga
     8161 cgaaagcgat cttaaacagc gcaatatcat tgccgtcgat gaacatatga ccattctcag
     8221 cgctctgatt tgccgcagtg accttgatgc cacccttgcg ctccgtaacc atttgaattc
     8281 agctaaacaa tcaatgattc gatccattaa tgagaacacg cgttatgctc attaagtacg
     8341 gattaaaaac agcaaactat tagcgggtgc acggttttat caaaagataa ataccgggaa
     8401 attcccgctt acctatgctc acaatcagtc gactaaaaat gtttttgtga gcggtagtaa
     8461 agtcctaaaa ctttaacctg ccgttaatgt caaccttgcg taatgcacaa ttacgtaaac
     8521 aattaaaatt ataaatacag gagtccggcg tggaaaaaga aaatatcacc ctcgatccgc
     8581 gttcttcatt tactccatct tcgtcggcag atattcccgt gccaccagat ggattagttc
     8641 aacgcagtac ccgaattaaa cgcattcaaa ccaccgccat gttgttatta ttttttgcgg
     8701 cggtaatcaa ttatctcgac cgcagttcgc tgtcggtagc aaatttaacg attcgtgaag
     8761 aattgggatt aagtgccacc gaaatcggcg ctttgctctc cgtgttttca ctcgcttacg
     8821 gcattgcgca acttccttgc ggcccactat tggatcgtaa aggcccacgc ctgatgctgg
     8881 gactggggat gttcttctgg tcactgtccc aggccatgtc tggcatggtg cacagcttta
     8941 cgcagttcgt gttggtgcgt atcggtatgg ggattggtga agcgccgatg aacccatgcg
     9001 gtgtaaaagt cattaacgac tggttcaaca tcaaagagcg cggacgcccg atgggcttct
     9061 tcaacgcagc ttctaccatt ggcgttgctg taagcccacc gattctggcg gcaatgatgc
     9121 tggtgatggg ctggcgcggg atgtttatta ccattggtgt actggggatt tttctcgcca
     9181 tcggctggta tatgctctat cgcaaccgcg agcacgtaga actgactgcc gttgaacaag
     9241 cttatctcaa tgcaggtagc gtcaatgccc gccgagatcc gctcaatttt gccgaatggc
     9301 gcagcctgtt ccgtaaccgc acaatgtggg gaatgatgct cggattcagt ggcatcaact
     9361 acactgcgtg gctgtatctg gcctggcttc ctggttacct gcaaacagcc tataacctgg
     9421 atttaaaaag cacagggttg atggcggcta tccctttcct gtttggggct gccgggatgc
     9481 tggtcaacgg ttacgttact gactggctgg tcaaaggggg aatggctccg attaaaagcc
     9541 gtaagatctg cattattacc gggatgttct gttctgccgc ctttacgctg atagtaccac
     9601 aagcgacaac atccatgatg gcggttctgc tgattggcat ggcactgttc tgtattcact
     9661 ttgccggaac atcctgctgg ggcttgatcc acgtcgcagt tgcttctcgc atgactgcgt
     9721 cggtgggcag tatccagaac tttgccagct tcatctgcgc ctctttcgcg ccgatcatta
     9781 ctggttttat tgttgataca acccactcat tccgtctggc actaatcatt tgcggttgcg
     9841 tcaccgcagc gggagcactg gcgtacatct tcctggttcg ccagccgatc aacgacccgc
     9901 gtaaagatta aactccctat gcccgataac attttgctta tcgggcattt ttatggcgat
     9961 taaaacgttt cccagttctc cccgctatct gccactgcca ttttacgcgg cgtagctggc
    10021 gtcacggttt ttaccacagc cgatgtttca cgctgctgtt gctgctgttg ctgaatccgg
    10081 aacactgcca cagcttcggt caggcgactg gcctgctctt ccagcgcggc ggcggcagcg
    10141 gcagattctt ccaccagcgc ggcgttctgt tgagttaccc ggtccatctc agcaaccgct
    10201 aagccaacct gatcgatacc acggctctgc tcatcagaag cagaagctat ttcgcccata
    10261 atgtccgtca cgcgggtcac agcactgaca atctccgcca ttgtttcccc ggcgctttcg
    10321 accagcgtag agccaacatc cactttcccc accgagtctt caatcaggct tttaatttca
    10381 cgaaccgcct gggcgctacg ctgggccaga ttacgcactt ctcccgcaac caccgcaaaa
    10441 ccacgtcctt gctcacccgc acgcgctgct tcaaccgccg cgttcaaagc cagaatattg
    10501 gtctggaagg taatgccgtc aattacgctg ataatatcgg cgattttctg cgaactggtg
    10561 gagatatcgc gcatagtctg caccacgtta tctaccactt tgccgccgcg ttgcgccgtt
    10621 tcagaagcac ttaatgccag atggctggcc tggcgcgcat tctcggcgtt ctgtttcacc
    10681 gttgcggtca gttgctccat gctggctgcc gtctcttcca gcgaagcggc ctgttgctcg
    10741 gtgcgcgaag agagatcatt attgccggta gcgatttcgc tggcaccgct atagatggca
    10801 ttggccccgt tgcgcacatc accgacggta cgcatcagct ctccctgcat atggcgcaaa
    10861 ctctctgcca gttgccccat ctcattagag ccatccacct caatcggttt caccagatcg
    10921 ccgcctgcaa tatgacgaat gctgtcaatc aggcgattca ttggcgctac cagcgaggct
    10981 ttaataccga accagacggc gaagatgacc gccagtacga cgatcatcac gcccaccaga
    11041 atccacatcg cctggctgta ggaggcattg ttatcgctga cggcgatatc atagagccga
    11101 tcgttttgct ccatgtaagc cacatactgc ttctcgaaac cgtcctgata tccctgggtc
    11161 ggctgatcaa agaactcgtt gattttgcct gcacctaaca gttggatcag ctccgccagc
    11221 gcattgtgat aaatatcgta attacgtttg atctctgccg ctgcggcggt gctctgacgc
    11281 gggtcacgcg gcaacgcttc gtaatccgcc cagttttttt ccgcctgttt cagcgaaata
    11341 ctggcactct gcatcagctc agcaacggtt gaaccgctac caatattatt ctgatccatc
    11401 atgtagcgga tacccgcgcg gttgagggtg ttacgcgtct gcaacaacgc gacccagctg
    11461 ccattcagcg tgggttgctg ctggcgaatg gtttgtaaaa cagtgaaatt ttctttgtca
    11521 ttctttaagg cattaaaaaa cagaccgcct gatgtcagtt gtaaaaggcc aaaaacggcc
    11581 aaaaccagca gtaagctggt cacgatcttg atacgtttta acatggtttc tctttcctgt
    11641 ggaccgatgc aagattttcg gcctggaaag caaaaacttt atgaaaaggt gtaacccgcc
    11701 atccatggaa aaaccaggcg ctggttatca gatactaaaa agttattcat tatcgcccgg
    11761 gatgtcttct tgtcgggaat tgcccagagc aataaattcc tctaacagat gggttaactg
    11821 ttgcattttc tcggcagtaa actgagcttc aatctgccga taggcttctt caatctgtgt
    11881 ctgagcgcgg ttatacagcg cctgcccctc tttggtcagc gagatataga gcttccgctg
    11941 atcattgatc ggctttaatc gcaacactaa accgtcgcgt tccatccgcg tgaggatccc
    12001 ggtcaggctt gggcgcaaaa tgcaggcgcg atacgccaga tcgtgaaaat ccattgatgg
    12061 gctttccgcc agaatacgca cgatgcgcca ctgttgctca gtcaaattat gccgtttaac
    12121 gattgggcga aaataactca tcgccgcttc tcgcgcctgg agcaacgcaa tggttagtga
    12181 gtcgtgcatt atctttcccc tgaaaaattc aaaatcatta atatagaaac aattaattat
    12241 cacgttaccg ggactgagct gcccccgcaa ttcttcattt tatcaaaatt gatgatattt
    12301 tattaactta ctgatattta atattttatt atctttaaat gtaaatagtt tgttaattag
    12361 atcacattta tatcacttac ttttgccaaa ctacgcaatc gggaataaaa gtaatcatta
    12421 acatattaat gattaagatg atcgaatccg aggagtggta atgaaaggca ctatcttcgc
    12481 cgtagcgttg aaccatcgca gccaacttga tgcctggcag gaagcgttcc agcaatctcc
    12541 ctacaaagcc ccgcctaaaa ctgcggtctg gtttattaaa ccgcgcaata cggtgattgg
    12601 ttgcggtgaa ccgattccct ttccacaggg tgaaaaagta ctgagcggtg cgactgttgc
    12661 gctgattgtg gggaaaacgg cgacgaaagt acgtgaagaa gatgcggcag agtacatcgc
    12721 cggatatgcg ctggctaacg acgtcagcct gccggaagag agcttttacc gtccggcaat
    12781 caaagcaaaa tgtcgtgatg gattctgccc cattggcgaa accgtggctc tcagcaatgt
    12841 cgataatctg actatctata ccgagatcaa cgggcgtcct gccgatcact ggaataccgc
    12901 cgatttacaa cgtaacgccg cacagctgct gagcgccctg agcgaatttg ccacgctgaa
    12961 tccaggcgat gctattctgc tcggcacgcc acaggcgcgc gtggaaatac agccaagcga
    13021 tcgcgtgcgt gttctcgcag aaggtttccc gccgctggaa aatccggtag tggacgaacg
    13081 tgaagtgacc acgcgcaaga gcttcccaac gctgccacac ccgcacggta cgctgtttgc
    13141 cctcggcctg aactacgccg accacgccag cgaactggaa tttaagccac cggaagagcc
    13201 gctggtgttc ctgaaagcgc cgaataccct cactggcgac aaccagacct ccgtgcgccc
    13261 gaacaatatt gaatacatgc actacgaagc ggagctggtg gtagttattg gcaagcaggc
    13321 gcgtaacgtc agcgaagccg atgccatgga ttatgtcgcg ggctacaccg tgtgtaacga
    13381 ctacgccatt cgcgactatc tggaaaacta ctaccgccct aacctgcggg tcaaaagccg
    13441 cgacggactg acgccgatgc tttcaaccat cgtgccgaaa gaggcgatcc cggccccgca
    13501 taatctgacc cttcgcacct tcgtcaacgg cgagttacgc cagcaaggca ccaccgccga
    13561 tctgatcttc agcgtgccct tcctgatcgc ctacttaagc gaatttatga ccctgaatcc
    13621 gggcgacatg atcgccaccg gcacaccaaa aggcttatct gacgtggtgc ctggcgatga
    13681 agtagtggtg gaagtagaag gcgtgggccg cctggtgaac cgaattgtga gtgaggaaac
    13741 agcgaaatga aaaaagtaaa tcattggatc aacggcaaaa atgttgcagg taacgactac
    13801 ttccagacta ccaatccggc aacgggtgaa gtgctggcgg atgtggcctc tggcggtgaa
    13861 gcggagatca atcaggcggt agcggcagcg aaagaggcgt tcccgaaatg ggctgatctg
    13921 ccgatgaaag agcgtgcgcg cctgatgcgc cgtctgggcg atctgatcga ccagaacgtg
    13981 tctgagatcg ccgcgatgga aaccgcggac acgggcctgc cgatccatca gaccaaaaat
    14041 gtgttgatcc cacgcgcttc tcacaacttt gaatttttcg cggaagtctg ccagcagatg
    14101 aacggcaaga cctatccggt tgacgacaag atgctcaact acacgctggt gcaaccggta
    14161 ggcgtttgtg cgctggcgtc gccgtggaac gtgccgttta tgaccgccac ctggaaggtc
    14221 gcgccgtgtc tggcgctggg caataccgcg gtgctgaaga tgtcggaact ctccccgctg
    14281 accgccgacc gcctgggtga gctggcgctg gaagctggta ttccggcggg cgtgctaaac
    14341 gtggtacagg gctacggcgc aaccgcaggc gatgcgctgg tccgtcatca tgacgtgcgt
    14401 gccgtgtcgt tcaccggcgg taccgccacc gggcgcaaca tcatgaaaaa cgccgggctg
    14461 aaaaaatact ccatggagct gggcggtaaa tcaccggtgc tgatttttga agatgccgat
    14521 attgagcgtg cgctggacgc cgccctgttc accatcttct caatcaacgg cgaacgctgc
    14581 accgccggtt cgcgcatctt tattcagcag agcatctatc cagaattcgt taaacgcttt
    14641 gccgaacgcg ccaaccgtct gcgcgtgggc gatccgaacg atccgaatac ccaggttagg
    14701 gcgcttatca gtcagcaaca ctgggaaaaa gtctccggct atatccgtct cggcattgaa
    14761 gaaggcgcaa ccctgttggc gggcggcccg gataaaccgt ccgacctgcc tgcgcacctg
    14821 aaaggcggca acttcctgcg cccaacggtg ctggcggacg tagataaccg tatgcgcgtt
    14881 gcccaggaag agattttcgg gccggtcgcc tgcctgctgc cgtttaaaga cgaagccgaa
    14941 ggcttacgcc tggcaaacga cgtggagtac ggcctcgcgt cgtacatctg gacacaggat
    15001 gtcagcaaag tgttacgcct ggcgcgtggc attgaagcag gcatggtgtt cgtcaacacc
    15061 cagaacgtgc gcgacctgcg ccagccattt ggcggcgtaa aagcctccgg caccgggcgt
    15121 gaaggcggtg agtacagctt cgaagtgttc gcggaaatga agaacgtctg catttccatg
    15181 ggcgaccatc caattccgaa atggggagtc tgatatgggt aagttagcgt tagccgccaa
    15241 gatcacgcac gtaccgtcaa tgtatctctc tgaactacca ggaaaaaacc acggttgtcg
    15301 ccagggtgcg atcgacgggc ataaagagat cagcaagcgt tgccgggaaa tgggcgtcga
    15361 taccattatc gttttcgata ctcactggct ggtcaacagt gcttatcaca tcaactgtgc
    15421 agaccatttt gaaggcgtct acaccagcaa cgagctgccg cattttattc gcgacatgac
    15481 ctacaactac gagggcaacc cggagttggg gcagctaatt gccgaggaag ccttaaagct
    15541 cggcgtgcgg gcaaaagcgc acaatattcc cagcctgaaa ctggaatacg gcacgctggt
    15601 accgatgcgc tacatgaatg aagacaagca cttcaaagtg gtctccattt cggctttctg
    15661 cacggttcac gattttgccg acagccgcaa gctgggcgaa gcgattctga aagcgatcga
    15721 acagtacgac ggcaccgtgg cggtccttgc cagcggttcg ttatcgcacc gctttattga
    15781 cgatcagcgt gcggaagaag ggatgaacag ctacacccgc gagttcgacc gccagatgga
    15841 cgagcgtgtg gtgaagctgt ggcgcgaagg ccagttcaaa gagttttgca atatgctgcc
    15901 ggagtacgcc gactactgct acggcgaagg caatatgcac gacacggtga tgctgctggg
    15961 gatgctcggc tgggataaat acgacggcaa ggtggagttt attaccgagc tgttcccaag
    16021 ctctggcacc ggtcaggtta acgctgtttt cccgctgccc gcgtaaggac gttttatgcc
    16081 gcactttatc gtggaatgta ctgaaaatat tcgtgaagaa gcccgcttac ctgagctgtt
    16141 tgccagtgtt aacactgcac tggcggcaac ggggattttt ccgcttgggg gaatacgtag
    16201 ccgggcgcac tggatcgata cctggcaaat ggctgatggc aagcacgatt acgcctttgt
    16261 gcatatgacg ctaaaaatcg gcagcggacg cagtctggaa agccgtcagg aagtgggcga
    16321 aatgctgttt gatctgatta aaacgcactt cgcctcactg atggagagcc gttacctagc
    16381 gctttcattt gagattgcag agctgcaccc gacgttgaat ttcaaacaga acaacgtaca
    16441 cgcgttgttt aaataaattt gcaccagctt ccaggccgga tgataaggcg ctgccgcatc
    16501 cggcaatgcc acaggatatc gctatgttcg ataaacacac ccacaccctg atcgcccagc
    16561 gtctggatca ggcagaaaaa cagcgcgaac agatccgcgc gatctcgctg gattacccgg
    16621 agatcaccat cgaagacgcc tacgccgtgc agcgcgaatg ggttcgcctg aaaatcgccg
    16681 aaagtcgcac gctgaaaggc cacaaaatcg gtctgacctc gaaagcgatg caggcaagct
    16741 cgcagatcag cgaaccggat tacggtgcac tgatggacga catgttcttc cacgatggca
    16801 gcgatatccc gaccgatcgc tttatcgtgc cgcgcattga agtggagctg gcttttgtgc
    16861 tggcaaaacc gctgcgtggg ccaaactgca cgctgttcga cgtttacaac gccacggact
    16921 atgtgatccc ggcgctggag ctgatcgacg ctcgctgcca caacatcgat ccggaaaccc
    16981 agcgcccgcg caaagtattc gacaccattt ccgataacgc cgccaacgcc ggagtgatcc
    17041 tcggaggtcg tccgatcaaa ccagacgaac tggatctgcg ttggatctcc gccctgatgt
    17101 atcgcaatgg cgtgattgaa gaaaccggcg tcgccgctgg tgtgctgaat catccggcaa
    17161 acggcgtggc ctggctggcg aacaaactcg caccgtatga cgtacaactg gaagccgggc
    17221 aaatcattct cggtggttcg ttcacccgcc cggttccggc gcgtaagggc gacaccttcc
    17281 acgtcgatta cggcaacatg ggctccatta gctgccgctt tgtttaagga gagaacgatg
    17341 gaaaacagtt ttaaagcggc gctgaaagca ggccgtccgc agattggatt atggctgggg
    17401 ctgagcagca gctacagcgc ggagttactg gccggaccag gattcgactg gttattgatc
    17461 gacggtgagc acgcgccgaa taacgtgcaa accgtgctca cccaactaca ggcgattgcg
    17521 ccctacccca gccagccagt ggtacgccca tcgtggaacg atccggtgca aatcaaacaa
    17581 ctgctggacg taggcacaca aaccttactg gtgccgatgg tacaaaacgc cgacgaagcc
    17641 cgtaaagcgg tacgcgccac ccgttatccc cccgccagta ttcgcggtgt gggcagtgcg
    17701 ctggctcgcg cctcgcgctg gaatcgcatt cctgattacc tgcaaaaagc caacgatcaa
    17761 atgtgcgtgc tggtgcagat cgaaacgcgt gaggcaatga agaacttacc gcagattctg
    17821 gacgtggaag gggtcgacgg cgtgtttatc ggcccggcgg atctgagcgc cgatatgggt
    17881 tatgccggta atccgcagca cccggaagta caggccgcca ttgagcaggc gatcgtgcag
    17941 atccgcgaag cgggcaaagc gccggggatc ctgatcgcca atgagcaact ggcaaaacgc
    18001 tatctggaac tgggcgcgct gtttgtcgcc gtcggcgttg acaccaccct gctcgcccgc
    18061 gccgccgaag cgctggcagc acggtttggt aatagttctt ctatttcgat ggcgaagcaa
    18121 aacaacaact ctgtctacta aacgtacgat gtggagcgca tcatgacgac ctctaccctg
    18181 caatataaag ataataaaac tgttgaaatt gaacatcgtg tcattaataa attatttcgt
    18241 cgcctaatca tttttctttt tattctgttt gttttttcgt ttcttgatcg catcaatata
    18301 ggatttgccg gactgacaat gggcaaggat cttggcctga cgtccacaat gtttggcctc
    18361 gccgcaacac tcttttatgt cacgtacgtc atgtgcggga tccccagtaa tatgatgtta
    18421 gcgaaaattg gcgctcgtcg atggatcgct gggatcatgg tggtctgggg aattgcttcc
    18481 acctgcacaa tgtttgcaac aagccccgaa acactctatc tactgcgtat gctggttggt
    18541 attgctgagg cgggtttttt acctggtatt ttggtttatc tgacctggtg gttcccggca
    18601 tatcatcggg ccagagccaa tgccttattt atgatagcga tgccagtcac tatgatgctg
    18661 ggattgatcc tttcaggcta cattctggcc ttggatggac tgtggaatct aaaaggctgg
    18721 caatggctct ttttgctgga aggtctgcca tcggtcattc ttggtgtcgt cacctggttt
    18781 tatctcaacg acacaccaga ccaggcgacc tggcttgatg aaaacgaaaa acaggcttta
    18841 aaaacgatga ttctccgtga acaagagagt gcatttgccc atgcatctgt tccacgctca
    18901 accttacgtg aagtcctgac cccagccatc atgctatata cacttgctta tttttgcctg
    18961 actaatactc tgagtgcgat taatatctgg acgccgcaaa tcctacagag tttcaatact
    19021 ggcagtagca acatcatgat tggtttgctg gcggccatcc cccaattctg cacaattttt
    19081 ggcatgatct ggtggagtcg tcgttccgac cgtcttaaag aacgcaaaaa gcatacgatt
    19141 cttccctacc tgtttgccgc tgccggatgg ttactcgctt ctgcgacaga tcacagcctc
    19201 attcaactga gcagcatcat tatggcctca atgggatcat ttaccgcgat gactatcttt
    19261 tggacaacac cggatcaagt tataagcttg caatcgcgtg ccgttgcgat ggctgtcatc
    19321 aatgctgtcg gcaatgtagg ttcagcagtc agtccattac ttatcggtat tttgaaagat
    19381 gcaaccggca gttttagctc aggtttgtgg tttgtagcag gattgctggt actgggcgcg
    19441 ttagtcatga cccgaatccc tatggggagt cgggaacact ctctttcatc tgaacttatc
    19501 gcaccaaaac cgcaataagg agtcatgatg tccagtgaag ctatctttga acatggttat
    19561 gaaaatattg atttggtccg ggaatatgat gcccgctacg ctggtgaaga tgtgcattat
    19621 gaaacatttg cccggttagc gaatttcttt ggacgcgata tgcgccctca ctggcacgat
    19681 agatattttc aactccatta tctggtcact ggacgcatta ctttacaact ggatgagcac
    19741 ttctattctg tgcgcgcacc gctttttgta ctaacaccac catcggtgcc acatactttc
    19801 tttactgatc ctgatagcga tgggcatgtt ctgaccgtac gtcaggagtt aatttggccc
    19861 ctggtagaaa agctctggcc tggtagtgga gatgccgcaa tgctacaagg tatctgtctg
    19921 tcactggagg gaatggaaca tacacaggcg gcattaaatc attattggcc gataatatcc
    19981 ctggagtttc aacaaaataa gcaaggacgg gaaattctat tgcaatcact ggcgcaaagt
    20041 atttttacaa tcctactgcg tgaagcacca ccaaatgata tttcaacatg cagcgtgcgc
    20101 ggcgagatgt gtttattcca gaagtttaat cggctgattg acgaaaaata tcgccaacat
    20161 ttaatggtcc ctgaatatgc aacaatgctc ggtatgagtg aatcacggct taccgagctg
    20221 tgccgacgtt ttgccaatca atcgccgaag agattgattt tcgaacgagt gagccgggaa
    20281 gccagacggc ttctgctcta tagcgagcaa agcatcaacc agatagccct tgatcttggc
    20341 tacaaagatc ccgcctattt cgcccgtttt ttcaaccgaa tgatgggttg cccaccaaca
    20401 caattccgtg gtcgctgacc atttatcaac ctgagagact cgacatgaaa atatctttac
    20461 gctcactatt tgtggcgtgc ctgattgcgc cagtggcatt cacctacgcc tccgaaccgc
    20521 aataaatgaa aattgtacta cttggaacga aaggtggccc ttctttatta aaagctgacc
    20581 gtttaccgat ggcaacggca cttattattg gcaataaaac ctagttaatt gatgctggtt
    20641 acggggccag cttacaactg gtaaaaaata atattccact gcgccagatt gataatattt
    20701 tactcaccca tttacacagt gatcatattc tggactatcc atcagtctta atgaatgcat
    20761 gggctagcgg tctgcaggac aaaactattc atgtattcgg tccgccaggc acccgagcca
    20821 tgaccgctgc cagttggcag gtcttcgatc gggacatcac actgcgtatg gaagaagaag
    20881 gaaaacccga tcctcgcaaa ctcgttacag ctcaagatgt tggtgaaggc gtagtttata
    20941 aagatgatac cgtgaccgtg actgcattaa aggtccctca ttctcctttc cctgacggtg
    21001 aggcttttgc ttatcgcttc gacacacaag gcaagcgaat tgtcttctca ggagatacct
    21061 cgtactttcc cccactggca actttctcag aaggcgctga tatacttgtg catgaggccg
    21121 tacatgagcc ctccgtcgct aagctggcag acagtattgg caatggtaaa acactggcaa
    21181 aagccattgc ttctcaccat acgactattg aggatgtagg caaaattgcc aatgaagcaa
    21241 aagtgaaaaa actcatcctt agccacctgg taccaatcac cgttccggat gaaacatgga
    21301 agcaagaagc acagaaaact attcaggccc tatcgtcgtt ggtcatgaaa atatgacgat
    21361 taccattcca ttcgccccat aaaaaattcc gtccccgtta gcccttgtta gcggggaata
    21421 ttatgatttg ccttactgta agttgatcac gatcgcagtt tactatttaa ctccaccttg
    21481 ctgtcgaaaa gtaccaacgt tcgcctgaaa cgtctcttcc cgacaaccgc gttcaacgct
    21541 tcaattaaat aacaaaaaca acacacaaaa ttaacaatcg tattccgatt aatactgtag
    21601 aggtcgacat gaaaccagaa gatttccgcg ccagtaccca acgtcctttc accggggaag
    21661 agtatctgaa aagcctgcag gatggtcgcg agatctatat ctatggcgag cgagtgaaag
    21721 acgtcaccac tcatccggca tttcgtaatg cggcagcgtc tgttgcccaa ctgtacgacg
    21781 cgctgcacaa accggagatg caggactctc tgtgttggaa caccgacacc ggcagcggcg
    21841 gctataccca taaattcttc cgcgtggcga aaagtgccga cgacctgcgc cagcaacgcg
    21901 acgccatcgc tgaatggtca cgcctgagct atggctggat gggccgtacc ccagactaca
    21961 aagccgcttt cggttgcgca ctgggcgcga atccgggctt ttacggtcag ttcgagcaga
    22021 acgcccgtaa ctggtatacc cgtattcagg aaactggcct ctactttaac cacgcgattg
    22081 ttaacccacc gatcgtcatt tgccgaccga taaagtgaaa gacgtttaca tcaagctgga
    22141 aaaagagact gacgccggga ttatcgtcag cggtgcgaaa gtggttgcca ccaactcggc
    22201 gctgactcac tacaacatga ttggcttcgg ctcggcacaa gtgatgggcg aaaacccgga
    22261 cttcgcactg atgttcgttg cgccaatgga tgccgatggc gtgaaattaa tctcccgcgc
    22321 ctcttatgag atggtcgcgg gtgctaccgg ctcgccatac gactacccgc tctccagccg
    22381 cttcgatgag aacgatgcga ttctggtgat ggataacgta ctgatcccat gggaaaacgt
    22441 gctgatctac cgcgattttg atcgctgccg tcgctggacg atggaaggcg gtttcgcccg
    22501 tatgtatccg ctgcaagcct gtgtgcgcct ggcagtgaaa ctcgacttca ttacggcact
    22561 gctgaaaaaa tcactcgaat gtaccggcac cctggagttc cgtggtgtgc aggccgatct
    22621 cggtgaagtg gtggcgtggc gcaacacctt ctgggcattg agtgactcga tgtgttcaga
    22681 agcgacgccg tgggtcaacg gggcttattt accggatcat gccgcactgc aaacctatcg
    22741 cgtactggca ccaatggcct acgcgaagat caaaaacatt atagaacgca acgttaccag
    22801 tggcctgatc tacctccctt ccagtgcccg tgacctgaac aatccgcaga tcgaccagta
    22861 tctggcgaag tatgtgcgcg gttcgaacgg tatggatcac gtccagcgca tcaagatcct
    22921 caaactgatg tgggacgcca ttggcagcga atttggtggt cgtcacgagc tgtatgaaat
    22981 caactactcc ggtagccagg atgagattcg cctgcaatgt ctgcgccagg cacaaagctc
    23041 cggcaatatg gacaagatga tggcgatggt tgatcgctgc ctgtcggaat acgaccagaa
    23101 cggctggact gtgccgcacc tgcacaacaa cgacgatatc aacatgctgg ataagctgct
    23161 gaaataacgc agcaggaggt taagatgcaa ttagatgaac aacgcctgcg ctttcatgac
    23221 gcgatggcca gcctgtcggc agcggtaaat attatcacca ccgagggcga cgccggacaa
    23281 tgcgggatta cggcaacggc tgtctgctcg gtcacggata caccaccgtc gctgatggtg
    23341 tgcattaacg ccaacagtgc gatgaacccg gtttttcagg gcaacggcaa gttgtgcgtc
    23401 aacgtcctca accatgagca ggaactgatg gcgcgccact tcgcgggcat gacaggcatg
    23461 gcgatggaag agcgttttag cctctcatgc tggcaaaaag gtccgctggc gcagccggtg
    23521 ctaaaaggtt cgctggccag tcttgaagga gagatccgcg atgtgcaggc gattggcaca
    23581 catctggtgt atctggtgga gattaaaaac atcatcctca gtgcagaagg tcacggactt
    23641 atttacttta aacgccgttt ccatccggtg atgctggaaa tggaagctgc gatttaagct
    23701 ccagcccctt cacgcattcg tcgaaggggc atttttattg tgatctcaat cacatatcta
    23761 cccgatgaat aattcttgcc caaccgctca ccgccattcc tgaccgctta catccctaaa
    23821 ataaccactc agttatttac cttactttac gcgcacgtat ctctggcaac atcactgcag
    23881 gatagcggtc aatttacctc ctcaaacgca acgcaaacct agaacggctt cggcctatga
    23941 ttaacaaatt actctatgga ttttggtttg caggaaggca gcaagtgagt gaatccccgg
    24001 gagcttacaa aagtaagtgt cagaggtgaa cgaacgcagc tgccgcacct gtaagccaaa
    24061 agacgacgag taaaatgcca ggttttacta tggatacgaa aaaactattc aagcacatac
    24121 cctgggtgat tctcggaatc atcggtgcat tctgcctcgc ggtagttgca ttacgtcggg
    24181 gggagcacgt cagcgccctg tggatcgtgg tcgcctctgt gtcggtgtat ctggtggcgt
    24241 atcgctacta cagtctgtac atcgcccaga aggtgatgaa actcgacccc acgcgcgcga
    24301 cgcctgcggt tattaacaac gacggtctga actacgttcc gaccaaccgt tacgtgttgt
    24361 ttggtcacca cttcgccgct atcgccggtg ctggtccgct ggtgggaccg gttctcgccg
    24421 cgcagatggg ctacctgcct ggcacgctgt ggctgctggc gggggtagtg ctggccggtg
    24481 cggtacagga ctttatggtg ctgtttatct cctctcgccg taacggcgca tctctcggtg
    24541 agatgatcaa agaagagatg gggccagtac cggggactat cgcgctgttt ggctgtttct
    24601 taatcatgat catcatcctc gccgttctgg cgctgattgt ggttaaagcc ctggccgaaa
    24661 gtccgtgggg tgtcttcacc gtttgctcaa ccgtaccgat tgcgctgttt atgggtatct
    24721 acatgcgctt tatccgtccg gggcgtgtgg gtgaagtctc tgttattggt atcgtgctgc
    24781 tggttgcctc tatctacttc ggtggcgtga ttgctcacga tccgtactgg ggtccggcac
    24841 tgacctttaa agacaccacc attaccttcg cgctgattgg ctatgcgttt gtttccgcac
    24901 tgctgccggt gtggctgatc ctcgcaccgc gcgactatct ggcaaccttc ctgaaaatcg
    24961 gcgttatcgt cggcctggcg ctgggtatcg tggtgctgaa cccggaactg aaaatgcctg
    25021 cgatgaccca gtacattgac ggtactggcc cgctgtggaa aggcgctctg ttcccgttcc
    25081 tgttcatcac catcgcctgt ggtgcggtat ctggcttcca cgcgctgatc tcttccggta
    25141 cgacgccgaa actgctggct aacgaaaccg acgcgcgttt cattggctac ggcgcaatgc
    25201 tgatggagtc cttcgtggcg attatggcgc tggttgcagc gtccatcatc gaaccgggtc
    25261 tttacttcgc gatgaacacc ccgcctgctg gccttggcat caccatgcct aacctgcatg
    25321 aaatgggtgg cgagaacgcg ccgatcatca tggcgcagct gaaagacgtt accgcacacg
    25381 cggcagcgac cgtcagctcc tggggcttcg tgatttcgcc agagcagatc ctgcaaaccg
    25441 cgaaagacat tggtgagcct tctgtcctga accgtgcagg tggtgcgccg acgctggcgg
    25501 taggtatcgc tcacgtgttc cacaaagtgc tgccgatggc tgacatgggc ttctggtatc
    25561 acttcggtat tctgttcgaa gccctgttca tcctgaccgc gctggatgcg ggtacccgtt
    25621 ctggccgctt tatgctgcaa gacctgctgg gtaacttcat cccgttcctg aaaaaaaccg
    25681 actctctggt tgccggtatc atcggtactg cgggctgtgt gggtctgtgg ggctacctgc
    25741 tgtatcaggg cgtggtcgat ccgctgggcg gcgttaagag cctgtggccg ctgttcggta
    25801 tctccaacca gatgctggca gccgtagcgc tggtactggg caccgttgtg ctgattaaga
    25861 tgaagcgcac ccaatacatc tgggtaactg ttgttccggc tgtatggctg cttatctgca
    25921 ccacctgggc gctgggtctg aaactgttca gcaccaaccc gcagatggaa ggcttcttct
    25981 acatggcaag ccagtacaaa gagaagattg ctaacggtac tgacctgacg gcgcagcaga
    26041 ttgccaacat gaaccacatc gttgtgaaca actacaccaa cgcaggcctg agtattctgt
    26101 tcctgattgt ggtgtacagc atcatcttct acggtttcaa aacctggctt gcggtgcgta
    26161 acagcgacaa acgtactgac aaagaaacac cgtacgttcc aatcccggaa ggcggcgtga
    26221 agatctcttc gcaccactaa ccgtgtttag ccccgcttcg gcggggcttt gttcttatca
    26281 gagtgaacta tgtttggtaa cttaggacag gcaaaaaaat atctcggcca ggcggcgaag
    26341 atgttgattg gtattccaga ctacgacaac tacgtcgagc atatgaagac caaccatccc
    26401 gacaagccgt acatgagcta tgaagaattc ttccgcgaac gccagaacgc gcgctacggc
    26461 ggcgatggta aaggcggtat gcgctgttgt taatggagag agcatgaacc cgattgcagt
    26521 taccctactc accggttttt taggcgcggg caaaactacc ctgctgcgcc atattcttaa
    26581 cgaacaacac ggctacaaga ttgccgtgat tgaaaacgaa ttcggcgaag tctccgttga
    26641 tgatcaactg attggcgacc gcgccacgca gatcaaaacg ctgaccaacg gctgcatctg
    26701 ctgttcgcgc tccaacgagc tggaggacgc actactcgac ctgctggata atctcgacaa
    26761 gggcaatatt cagttcgatc gtctggtgat tgaatgcacc ggcatggccg atcccggccc
    26821 gattattcag acctttttct cccatgagat tttatgccag ctctacctgc tggacggcgt
    26881 gattgcgctg gtggatgcgg tacatgccga tgagcagatg aaccagttca ccatcgccca
    26941 gtcccaggtg ggctacgccg accgtattct gctgactaaa accgacgtcg caggcgaagc
    27001 agagaaactg cgtgaacgcc tggcgcgcat caacgcccgc gcaccggtct acaccgtcac
    27061 ccacggcgac atcgacctgg gcctgctgtt caacaccaac ggttttatgc tggaagagaa
    27121 cgtcgtcagc accaaaccgc gtttccactt tatcgcggat aaacaaaacg atatttcgtc
    27181 aattgtggtg gaactggatt acccggtaga tatcagcgaa gtttcccgcg tgatggaaaa
    27241 cctgctgctg gaatcggcgg ataaactgct gcgttacaaa gggatgctgt ggattgacgg
    27301 cgaacctaac cgcctgctgt tccagggcgt ccagcgcctc tacagcgccg actgggacag
    27361 gccgtggggc gatgaaaaac cgcatagcac aatggtgttt atcgggattc agttgccgga
    27421 ggaagagatt cgggcggcgt ttgcgggatt gaggaagtaa gttcgggaaa gatcaacttt
    27481 ttgagagttc caccgcaaca ttattacgag tggaactctt tagatatgtc agaccaacca
    27541 aagcttcttc aattgcgttt tcattttttc atgtctcatt ttcagttcag ccaatcccca
    27601 ttctgaagaa ttacaaacat cttttgttaa ataaaaagag cttttactat taagtttctc
    27661 ataaatagat ttctttttga taaaatcata gtgttgtgtt tcagaatttt tggaaccact
    27721 tatgagtgtt aaattaccta ttttatgcag ccagtagaca tgctcttgag gttggaatct
    27781 attaatccag tattcattaa ctaatgcttg aggaagaata tgctcaattg tgattctccc
    27841 cgtataggtt ttaatcacgc tctcatcttg ttgctccata tctaagcgaa gcaaaattgc
    27901 ttttattaag ttcacttgat tcggacgagg ctcatataaa tcttcatcca gagcagctat
    27961 aaaacctgaa ttatctgcat gttggtttat ctgatttatc acagaatcaa aaggcatatc
    28021 attatttatt gctaccagag cagaataaca aaccatttct ctttggcttt taatttgttt
    28081 ttttaaccac ccatgcatat ataccttctc aaaggcggta ataaattgag aaaaatcatc
    28141 taaattaaaa tcttctgtac gagccattcg attcatgaat gccataaccg gaggaatcca
    28201 ctcatctacc cctaaattac ttagcgatgc tatttttcgt cgtattgaag ggtgttcaaa
    28261 atcattctct aaaatcttag tataattt
//
LOCUS       DAEEGA010000053        27601 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEEGA010000053 DAEEGA010000000
VERSION     DAEEGA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.053"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="KLH46_002419"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="KLH46_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBE0379069.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="KLH46_002420"
     CDS             complement(1614..1937)
                     /locus_tag="KLH46_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBE0379070.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="KLH46_002421"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="KLH46_002421"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBE0379071.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="KLH46_002422"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="KLH46_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBE0379072.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="KLH46_002423"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="KLH46_002423"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBE0379073.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="KLH46_002424"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="KLH46_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBE0379074.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="KLH46_002425"
     CDS             7537..8574
                     /locus_tag="KLH46_002425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBE0379075.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="KLH46_002426"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="KLH46_002426"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBE0379076.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="KLH46_002427"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="KLH46_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBE0379077.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="KLH46_002428"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="KLH46_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBE0379078.1"
                     /translation="MTPILLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="KLH46_002429"
     CDS             10745..11626
                     /locus_tag="KLH46_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0379079.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="KLH46_002430"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="KLH46_002430"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBE0379080.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="KLH46_002431"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="KLH46_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBE0379081.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="KLH46_002432"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="KLH46_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="KLH46_002433"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="KLH46_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBE0379082.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="KLH46_002434"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="KLH46_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBE0379083.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="KLH46_002435"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="KLH46_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBE0379084.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="KLH46_002436"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="KLH46_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBE0379085.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="KLH46_002437"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="KLH46_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBE0379086.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="KLH46_002438"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="KLH46_002438"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBE0379087.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="KLH46_002439"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="KLH46_002439"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBE0379088.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="KLH46_002440"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="KLH46_002440"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBE0379089.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="KLH46_002441"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="KLH46_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBE0379090.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="KLH46_002442"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="KLH46_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBE0379091.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="KLH46_002443"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="KLH46_002443"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBE0379092.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="KLH46_002444"
     CDS             24893..25273
                     /locus_tag="KLH46_002444"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBE0379093.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="KLH46_002445"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="KLH46_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBE0379094.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="KLH46_002446"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="KLH46_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBE0379095.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="KLH46_002447"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="KLH46_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6812 a         6867 c         7233 g         6689 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 acgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cttggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgaggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtacgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagcaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAEEGA010000054        27449 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEEGA010000054 DAEEGA010000000
VERSION     DAEEGA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27449
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.054"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            108..1139
                     /gene="mdtN"
                     /locus_tag="KLH46_002448"
     CDS             108..1139
                     /gene="mdtN"
                     /locus_tag="KLH46_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBE0379096.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWLVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1139..3187
                     /gene="mdtO"
                     /locus_tag="KLH46_002449"
     CDS             1139..3187
                     /gene="mdtO"
                     /locus_tag="KLH46_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBE0379097.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYG
                     EPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIV
                     VGLYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALA
                     LQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLA
                     SEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAA
                     KPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGS
                     SYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSS
                     YIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTL
                     PQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDS
                     EERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLA
                     TALSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ
                     "
     gene            3184..4650
                     /gene="mdtP"
                     /locus_tag="KLH46_002450"
     CDS             3184..4650
                     /gene="mdtP"
                     /locus_tag="KLH46_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBE0379098.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4848..5087
                     /locus_tag="KLH46_002451"
     CDS             4848..5087
                     /locus_tag="KLH46_002451"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379099.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5097..5267
                     /locus_tag="KLH46_002452"
     CDS             5097..5267
                     /locus_tag="KLH46_002452"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBE0379100.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5316..6728
                     /gene="fdhF"
                     /locus_tag="KLH46_002453"
     CDS             5316..6728
                     /gene="fdhF"
                     /locus_tag="KLH46_002453"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBE0379101.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6729..6995
                     /locus_tag="KLH46_002454"
     CDS             6729..6995
                     /locus_tag="KLH46_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HBE0379102.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7089..7778
                     /locus_tag="KLH46_002455"
     CDS             7089..7778
                     /locus_tag="KLH46_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBE0379103.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7856..9169)
                     /gene="gltP"
                     /locus_tag="KLH46_002456"
     CDS             complement(7856..9169)
                     /gene="gltP"
                     /locus_tag="KLH46_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBE0379104.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9511..10107)
                     /gene="nrfG"
                     /locus_tag="KLH46_002457"
     CDS             complement(9511..10107)
                     /gene="nrfG"
                     /locus_tag="KLH46_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBE0379105.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10104..10487)
                     /gene="nrfF"
                     /locus_tag="KLH46_002458"
     CDS             complement(10104..10487)
                     /gene="nrfF"
                     /locus_tag="KLH46_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBE0379106.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10480..12198)
                     /locus_tag="KLH46_002459"
     CDS             complement(10480..12198)
                     /locus_tag="KLH46_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBE0379107.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLCLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNCATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12218..13174)
                     /gene="nrfD"
                     /locus_tag="KLH46_002460"
     CDS             complement(12218..13174)
                     /gene="nrfD"
                     /locus_tag="KLH46_002460"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBE0379108.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13171..13842)
                     /gene="nrfC"
                     /locus_tag="KLH46_002461"
     CDS             complement(13171..13842)
                     /gene="nrfC"
                     /locus_tag="KLH46_002461"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBE0379109.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHVPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13839..14405)
                     /gene="nrfB"
                     /locus_tag="KLH46_002462"
     CDS             complement(13839..14405)
                     /gene="nrfB"
                     /locus_tag="KLH46_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBE0379110.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14450..15886)
                     /gene="nrfA"
                     /locus_tag="KLH46_002463"
     CDS             complement(14450..15886)
                     /gene="nrfA"
                     /locus_tag="KLH46_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBE0379111.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16279..18237
                     /gene="acs"
                     /locus_tag="KLH46_002464"
     CDS             16279..18237
                     /gene="acs"
                     /locus_tag="KLH46_002464"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBE0379112.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18437..18751
                     /locus_tag="KLH46_002465"
     CDS             18437..18751
                     /locus_tag="KLH46_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBE0379113.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18748..20397
                     /gene="actP"
                     /locus_tag="KLH46_002466"
     CDS             18748..20397
                     /gene="actP"
                     /locus_tag="KLH46_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBE0379114.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20575..21867
                     /locus_tag="KLH46_002467"
     CDS             20575..21867
                     /locus_tag="KLH46_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBE0379115.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22021..23670)
                     /locus_tag="KLH46_002468"
     CDS             complement(22021..23670)
                     /locus_tag="KLH46_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBE0379116.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23821..25170)
                     /gene="ghxP"
                     /locus_tag="KLH46_002469"
                     /gene_synonym="yjcD"
     CDS             complement(23821..25170)
                     /gene="ghxP"
                     /locus_tag="KLH46_002469"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBE0379117.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25876..26340)
                     /gene="soxR"
                     /locus_tag="KLH46_002470"
     CDS             complement(25876..26340)
                     /gene="soxR"
                     /locus_tag="KLH46_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBE0379118.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26426..26749
                     /gene="soxS"
                     /locus_tag="KLH46_002471"
     CDS             26426..26749
                     /gene="soxS"
                     /locus_tag="KLH46_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBE0379119.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26752..27449)
                     /gene="pdeC"
                     /locus_tag="KLH46_002472"
                     /pseudo
     CDS             complement(26752..27449)
                     /gene="pdeC"
                     /locus_tag="KLH46_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
BASE COUNT         6822 a         7438 c         6990 g         6199 t
ORIGIN      
        1 gggcaaatat caatctggca tttgtcggaa ttatttatac cgcccttttt gctctctacg
       61 ccatgctgtt ctggctggca tttttctaat ctatttgaga cgcgctcatg gaaagtacgc
      121 cgaaaaaagc acctcgcagt aaattccctg ctctgttagt ggttgcgttg gcgctggttg
      181 cccttgtttt cgttatctgg ctcgtagaca gtgcgccatc aactaatgac gcttacgcgt
      241 cagcagatac cattgatgtg gtgccggaag tcagcggtcg cattgtagaa ctggcggtca
      301 ccgacaacca ggcggtgaaa cagggcgatt tgctgttccg catcgacccg cgcccgtacg
      361 aagccaacct ggcgaaagcc gaagcctccc tcgcggcgct ggataagcaa attatgctca
      421 cccagcgtag cgttgacgcc caacagtttg gtgccgactc ggttaatgcc acggtagaaa
      481 aagcccgtgc cgccgcgaaa caggccacag atacattacg ccgcaccgag ccattactga
      541 aagagggttt tgtctcagca gaagacgttg accgtgcaag aacggcgcag cgcgccgcag
      601 aagcggatct taatgccgta ttgttacagg cgcagtcagc cgccagcgcc gtcagcggcg
      661 tcgatgcgct ggtggcgcaa cgtgcggcgg tcgaagcgga tattgccctg accaaactac
      721 atctggaaat gacgaccgtt cgcgcgccat ttgatggccg gatcatttcc ctcaaaacct
      781 ccgtcgggca atttgcttct gccatgcgcc ctatttttac cctcatcgac actcgtcact
      841 ggtacgtgat cgccaacttc cgcgaaaccg atctgaaaaa tattcgctca ggtacacccg
      901 caacgattcg cctgatgagt gacagcggca aaaccttcga gggtaaagtg gattcgattg
      961 gctacggcgt gctaccggat gacggcggcc tggtgctggg cggcctgcca aaagtgtctc
     1021 gttctattaa ctgggtccgc gttgcccagc gttttccggt caaaatcatg gtcgataaac
     1081 ctgacccgga aatgttccgc atcggcgctt cggcagtcgc taatcttgag ccgcaataat
     1141 gagcgcgctc aactccctgc cattaccggt ggtcaggctg ctggcgttct ttcatgaaga
     1201 gttaagcgag cggcgacccg gccgcgtgcc gcagatcgtg caactctggg taggctgcct
     1261 gctggtgatt ctgatctcga tgacttttga gatccctttt gtggcgttat cgctggcagt
     1321 gctgttttac ggtatcggta ttcagtcgaa cgcgttttac accaaatttg tggcgatctt
     1381 gtttgtggtt gccacggtgc tggagatcgg cagcctgttt ttgatctaca aatggtcata
     1441 cggcgaaccg ttgatccgat tgatcatcgc cgggccgatc ctgatgggct gcatgttttt
     1501 gatgcgcacc catcggctgg gactggtctt tttcgctgtc gccatttacg ggcaaacctt
     1561 ccccgccatg ctcgactatc cggaagtagt cgtgcgctta acgctgtggt gtatcgttgt
     1621 tggcctctat ccaaccttgc tgatgacgtt aatcggcgtg ctgtggtttc ccaatcgtgc
     1681 cattacgcaa atgcatcaag cgcttaatga tcggcttgat gatgccatta gccacctgac
     1741 ggacagcctc gcaccgctac ccgaaacgcg gattgaaaga gaggcgctgg cgctgcaaaa
     1801 actgaatgtc ttttgtctcg cggacgatgc caactggcga actcaaagcg catggtggca
     1861 aagctgcgtg gcaacggtaa cctacattta ctcgacgctg aatcgctacg atcccacctc
     1921 ttttgctgat tctcaggcaa ttattgaatt tcggcaaaaa ttagcttcag aaatcaacaa
     1981 gctgcagcat gccgttgctg aaggtcagtg ctggcaaagc gactggcgga tcagtgaaag
     2041 tgaagcggtg gcggcacggg aatgtaacct ggagaatatc tgccagacgt tgttacaact
     2101 gggccagatg aacccgaata cgccgccaac gcccgccgcc aaaccgccat caatggtcgc
     2161 cgatgctttt accaatccag actatatacg ctacgcggta aaaacattgc tcgcctgttt
     2221 gatctgttac accttttaca gcggcgtgga ctgggaaggc attcacacct gtatgctgac
     2281 ctgcgtgatc gtcgctaatc caaatgtcgg ttcgtcgtac cagaagatgg tgctgcgttt
     2341 tggcggggcc ttttgcggcg cgattctggc gctgttattc acgctactgg tcatgccctg
     2401 gctggacaat attgtcgaat tgctgtttgt gctggcaccg attttcctgt tggccgcatg
     2461 gattgccacc agctctgaac gctcttctta tatcggcaca cagatggtgg tcaccttcgc
     2521 gctcgccacg ctcgaaaacg tttttggccc agtgtacgac ctggtggaaa ttcgcgatcg
     2581 cgccctgggt atcatcattg gtaccgtggt gtccgcgatg atttacacct ttgtctggcc
     2641 tgaaagtgaa gcgcgcacgc tgccgcaaaa actggctggc gcgctgggta tgttaagtaa
     2701 agtaatgcgg atcccacgcc agcaggaagt cacggctctg cgcacttatc tgcaaattcg
     2761 tatcggtctg catgcggcgt ttaatgcctg tgaagagatg tgccaacgcg tggcgctgga
     2821 gcgtcaactg gacagcgaag aacgcgcctt actgattgaa cgttcgcaaa cggttattcg
     2881 tcagggccgc gatatccttc acgcctggga tgccacctgg aattcggcgc aggcgctgga
     2941 taacgcacta cagccggaca gagcaggtca gtttgccgac gccctggaga aatacgctgc
     3001 cggtctggca accgcactca gccgttctcc tcaaataacg cttgaagaga cacccacctc
     3061 gcaggccatc ctgcccacct tattaaaaca ggagcaacac gtctgccagc ttttcgcccg
     3121 cttgccagac tggacagccc cggcattaac gcccgccacg gaacaggcac aaggagccac
     3181 gcaatgatca atcgtcaact ttcacgtctg ctgttgtgca gcattctcgg cagcacgacg
     3241 ctgatttccg gctgtgccct ggtacgtaag gattctgcgc ctcatcaaca gctcaaaccg
     3301 gaacaaatca aactggccga tgatattcat cttgccagct ccggctggcc gcaggcgcag
     3361 tggtggaaac aactcaatga cccgcagttg gatgccctga tccaacggac gctaagtggt
     3421 tcacacaccc tcgccgaagc gaaactgcgg gaagaaaaag cgcaatcgca ggccgatttg
     3481 ttagatgccg gttcacagtt gcaggtggca gcgttaggga tgcttaaccg ccaacgtgtc
     3541 tcggcgaacg gctttttaag cccttatgcg atggatgcgc ccgcactggg gatggacggg
     3601 ccgtactata cggaagccac agtaggtttg tttgccggac tggatcttga tttgtggggt
     3661 gtgcatcgct cagcggttgc cgccgccatt ggcgcgcata atgccgcgct ggcagaaacc
     3721 gcagcagtag agctatcgct gaccacgggc gtagcgcagc tttattacag tatgcaggcc
     3781 agctatcaga tgctcgatct gttagaacaa actcgcgatg tgattgatta cgcggtgaaa
     3841 gcgcaccaaa gtaaagtggc gcacggtctg gaagcacaag tgcctttcca cggcgcgcgg
     3901 gcacagattc tggcggtcga taaacaaatt gctgccgtca aagggcaaat caccgaaacg
     3961 agagaatctc tgcgcgcatt gattggcgcg ggagccagcg atatgccgga gatcaaacct
     4021 gtggcattac cacgagtcca gaccggcatt ccggcgacac tctcttatga gttgctcgcc
     4081 agacgcccgg atctgcaagc catgcgctgg tatgttcagg cgtcattaga tcaggtggat
     4141 tccgcgcggg cgttgttcta tccgagcttt gatatcaaag cgtttttcgg ccttgatgcc
     4201 atccacctgg ataccttatt caaaaaaacc agtcgccagt tcaacttcat cccgggtctg
     4261 aaattgccgc tgtttgacgg tggacggttg aatgccaatc tcgaaggcac gcgcgccgcc
     4321 agcaacatga tgattgaacg ttacaaccag tcagtactga acgcggtgcg tgacgttgcc
     4381 gtcaacggca cgcgtctgca aacgctcaac gacgagcgag aaatgcaggc tgaacacgtg
     4441 gaagcaacgc gcttcaccca gcgcgctgcc gaggccgcct atcagcgcgg cttaaccagc
     4501 cgcttacagg ccactgaagc ccggttgcca gtacttgccg aagagatgtc attactgatg
     4561 ctggacagcc gccgggtgat ccaaagcatt cagttgatga aatcgctggg cggcgggtat
     4621 caggcggctc ccgtcgtcga gaaaaaataa aatgtctgcc gcgtgatggc tgtcacgcgg
     4681 tatttcgttt cgtcacgtca aaactgacga cagcctgttt ttcgtcagag ttttgaataa
     4741 atagtgcccg taatatcagg gaatgacccc aaataaaatg tggcataaaa gatgcatact
     4801 gtagtcgaga gcgcgtatgc gtgatttgat taactggagc gagaccgatg aaaaaagtcg
     4861 tcacggtttg cccctattgc gcatcaggtt gcaaaatcaa cctggtcgtc gataacggca
     4921 aaatcgtccg ggcggaggca gcgcagggga aaaccaacca gggtaccctg tgtctgaagg
     4981 gttattatgg ctgggatttc attaacgata cccagatcct gaccccgcgc ctgaaaaccc
     5041 ccatgatccg tcgccagcgt ggcggcaaac tcgaacctgt ttcctgagat gaggcactga
     5101 attacgttgc cgagcgcctg agcgccatca aagagaagta cggtccggat gccatccaga
     5161 cgaccggctc ctcgcgtggt acgggtaacg aaaccaacta tgtaatgcaa aaatttgcgc
     5221 gcgccgttat tggtaccaat aacgttgact gctgcgctcg tgtctgacac ggcccatcgg
     5281 ttgcaggtct gcaccaatcg gtcggtaatg gcgcaatgag caatgctatt aacgaaattg
     5341 ataataccga tttagtgttc gttttcgggt acaacccggc ggattcccac ccaatcgtgg
     5401 cgaatcacgt aattaacgct aaacgtaacg gggcgaaaat tatcgtctgc gatccgcgca
     5461 aaattgaaac cgcgcgcatt gctgacatgc acattgcact gaaaaacggc tcgaacatcg
     5521 cgctgttgaa tgcgatgggc catgtcatta ttgaagaaaa tctgtacgac aaagcgttcg
     5581 tcgcttcccg tacagaaggc tttgaagagt atcgtaaaat cgttgaaggc tacacgccgg
     5641 agtcggttga agatatcacc ggtgtcagcg ccagtgagat tcgtcaggcg gcacggatgt
     5701 atgcccaggc gaaaagcgcc gccatcctgt ggggcatggg tgtaacccag ttctaccagg
     5761 gcgtggaaac cgtgcgttct ctgaccagcc tcgcgatgct gaccagtaac ctcggtaagc
     5821 cgcatgcggg tgtaaacccg gttcgtggtc agaacaacgt gcagggtgcc tgcgatatgg
     5881 gcgcgctgcc ggatacgtat ccgggatacc agtacgtgaa agatccggct aaccgcgaaa
     5941 aattcgccaa agcctggggc gtggaaagcc tgcctgctca taccggctat cgcatcagcg
     6001 agctgccgca ccgcgcagcg catggcgaag tgcgtgccgc gtacattatg ggcgaagatc
     6061 cgctgcaaac tgatgcggag ctgtcggcag tacgtaaagc ctttgaagat ctggaactgg
     6121 tcatcgttca ggacatcttt ataaccaaaa ccgcgtcggc ggcggatgtc attttgccgt
     6181 caacgtcgtg gggcgagcat gaaggcgtgt ttactgcggc tgaccgtggc ttccagcgtt
     6241 tcttcaaagc agttgaaccg aaatgggatc tgaaaacgga ctggcaaatc atcagtgaaa
     6301 tcgccacccg tatgggttat ccgatgcact acaacaacac ccaggagatc tgggatgagt
     6361 tgcgtcatct gtgcccggat ttctacggtg cgacttacga gaaaatgggc gaactgggct
     6421 tcattcagtg gccttgccgc gatacttcag atgccgatca ggggacttct tatctgttta
     6481 aagagaagtt tgataccccg aacggtctgg cgcagttctt cacctgcgac tgggtagcgc
     6541 caatcgacaa actcaccgac gagtacccga tggtactgtc aacggtgcgt gaagttggtc
     6601 actactcttg ccgttcgatg accggtaact gtgcggcact ggcggcgctg gctgatgaac
     6661 ctggctacgc acaaatcaat accgaagacg ccaaacgtct gggtattgaa gatgaggcat
     6721 tggtttgagt gcactcgcgt aaaggcaaaa ttatcacccg tgcgcaggtc agcgatcgtc
     6781 cgaacaaagg ggcgatttac atgacctacc agtggtggat tggtgcctgt aacgagctgg
     6841 ttaccgaaag cttaagcccg attacgaaaa cgccggaata caaatactgc gccgtgcgcg
     6901 tcgagccgat ctccgatcag cgcgccgccg agcagtacgt gattgacgag tacaacaagt
     6961 tgaaaactcg cctgcgcgaa gcggcactgg cgtaatacag tcctttctac agcctccttt
     7021 cggaggctgt ttttttatcc attcgaactc tttatactgg ttactcccta cccaatcgta
     7081 ttatcaaaat gaaaaaaatt atcgcattga tgttgttttt gacattcttt gcccacgcga
     7141 acgactccga gcctggcagc cagtatttaa aggcagcaga ggccggggac cgacgtgcac
     7201 aatattttct tgccgacagc tggtttagct ccggcgattt gagcaaagcc gaatattggg
     7261 cacagaaagc cgccgacagc ggtgatgctg atgcctgcgc gctgttggcg cagatcaaaa
     7321 tcaccaatcc ggtcagtctg gactatccac aagcaaaagt tcttgcagag aaagcggcgc
     7381 aagcgggcag taaagaaggt gaagtaacgc tggcgcatat tctggtaaat actcaagcgg
     7441 gtaaaccgga ttatccaaag gcgatttcgc tgttagaaaa cgcctcggaa gatctggaga
     7501 atgactccgc cgtcgatgcc caaatgctgc ttggtttgat ttacgccaac ggcgtgggca
     7561 ttaaggccga cgatgacaag gcaacctggt atttcaaacg cagctctgca atttcccgaa
     7621 ccggttattc cgagtactgg gcgggaatga tgttcttaaa cggtgaggaa ggttttatcg
     7681 agaagaacaa acaaaaggcg ctgcactggt tgaacctgag ctgtatggaa gggtttgata
     7741 ccgggtgtga agagtttgaa aaattaacca acggttaagg taggcctgat aagacgcggc
     7801 aagcgtcgca tcaggcatcc acacattgcc gggggatatc cccggcaatc ttcaattatt
     7861 gatccgcagt tttatcaaat ttgcccagca cttcacgctc ataagccagc gcctttttgc
     7921 ggtcaaattt gtgttcccac ttggcaatca ccagcaccgc cagcgcatta cccaccacgt
     7981 tcagcgcagt acgcgccatg tcgaggatac ggtcaacacc agcaataaac gccagacctt
     8041 ccagcggaat acctacgcta cccagcgttg ccagcaacac cacaaacgac acgccagcca
     8101 cgccagcaat ccctttcgag gtcaccatca gcgtcagcac cagaatgatt tcctgccaga
     8161 tggacaggtc aatgccatac agctgagcga tgaaaatagc ggcaatactt tgatacagcg
     8221 tcgaaccatc aaggttaaaa gagtaaccgg tcggcaccac gaagctggtg atcgacgccg
     8281 gtgctccgta ggcttccatc ttctcaataa ttcgcggcag tacgctttca gagctggcag
     8341 tggagtacgc cagaatcagc tcatctttca gaatacgaat caggatccag acgcttaacc
     8401 cgcacagacg tgccacaatt cccagcacta ccagcgcgaa gaacagaatg gcgaaatgca
     8461 ccagcagcac cagtttcgcc agtggccaca gagacgagaa accaaagtta gccaccgtca
     8521 ccgcaatcag cgcaaacaca cccaccggtg cataacgcat caccatgtga gtcactttaa
     8581 acatggtttc agagatggag cggaacacgg tcaccagcgg ttcacgatgc gtcgcgggca
     8641 gggaagaaag ccccagacca aacagcaccg agaaaaagat gatcggcagc atttcgcctt
     8701 tcgccatcga cgccacaatg ttcgtcggca ccagcgacaa aatcgtgccc ataatgccgt
     8761 gggaactgct ttgtaccgct tccgtagtgc tctgatattt cgagatatcg acggtcgcca
     8821 gctgcgacat atccaccccg gcaccgggct ggaagacgtt cgccagagtg atccccaaaa
     8881 tgatggcgac ggtggtgatc acctcgaagt aaataatggt tttcgcgcca atacgcccga
     8941 gctgtttggc atcaccaaca cccgcgatac ccaccaccag cgtggagatc acaatcggca
     9001 caacaatcat tttgatcaga tggatgaaga tatcacccgc cggagagagc aaattgacga
     9061 ccagccagtc gcggctgtcg ctatggtagt gcaggtagct tcccaggaga atgcccagca
     9121 ccatagcaaa cagaatctgc caggccaggc tgaattttat atttttcatc agaataactt
     9181 cctcaatgga gcgccgttcc ccgttcagaa acgcgagaac gtgacatact gccagggcat
     9241 gaattgtgtt gcgttggttt gtagattttt ttaacgctta tgatcagtat catttgcgcg
     9301 gtatgttgcg caacccttta acaacagagc ttttcgctgc attaatgcat aataacgcga
     9361 gtttatggca ggattaatga ataaccattt attatggaaa gatttttatt tgtttaatct
     9421 gcaataatac tgattggtga ttatttttgg catatgttca ttcgattatt ttttatgcag
     9481 ctcaatgaca taatcttcat caccagatga ttaatccgat ctccgctgca acagttttgc
     9541 catattaatc gactcgacca gctgtgtccg gttgatccgg ggtgagttga gatccatcac
     9601 tttttgccat agttcgatgg cttgcgcgta attcgcctgc ataaacgcat cggaagccag
     9661 cagcatcaga gcggtgattt cgttagagtc cagcgcgagg gctttgtcga tcattgcgcg
     9721 agtctgggcg gtcatatgct ggctggcctg gtaataaagc accgtcgcca gcgccgcata
     9781 cagttcagcg ttctcaccac gtagttgcaa cgcctgacga tacgccagca gcgaattgct
     9841 gtaatcgttt tgccacagat agtactcgcc cagtaacgcc cattgttcgc tgttttgtgg
     9901 attagcgcgg attttatcct gcaatgcctg aagctgcgct tccggggttt gctggctggc
     9961 aaactgatgt agcggatcgc gctgacgctg atactccgca cgtaccgcct gccattttgg
    10021 gcttaacaga taactgccga cacagaggaa taccatcaat atcgtgagtg ttgtcagcat
    10081 tttcaccggt atttgaggct gtttcatcgc ttagccctca ctcgccagag gatcagtgcc
    10141 atcagcaata acaacaccac tggcagcgcc cacaacacca gcgtctgacc cgttaacggt
    10201 gggttatagc gaacaaaatc tccgtagcgt tcggtcatcc agccaatgat ttcgacttcg
    10261 ttcttcccct ccgacaccat gctataaacc tgatggcgca tactgaccgc caccggcgca
    10321 ttggattcga gtaagttttg attctggcac tgcggacaac gtaactggct ggcaatattt
    10381 aacgcctgtt gctgttgttg cggattggcg aattgccagg tgtctacaac ctgagcgcgc
    10441 gcaaaacagg taaataacag cagtagcgtg agaagccctt tattcatcgc gcttcttccc
    10501 ccgccatccg cttaacaatg cgcccgcaat catcaacaat cctccccccc agatccagcg
    10561 cacaccgctt tgtacataca aacgaaaagc gtaacgatcc gctctggttt tttcacccat
    10621 gaccgcatac cagtcatgga tgccgttcca gcgaattgac ggttccatca tttgctgacg
    10681 gcgtgcttca taaaaacgcc gctccggcgt taattcacca atgcgttgct gatggtcaaa
    10741 cagcgccact atcgcttttt cgctggtgta attgcctctg gcttgcagat cgagtcgctc
    10801 aaaacggaag gtgtatcctg ccagcgtcac ctgctgaccc ggctgtaaat tcaggctgat
    10861 ctcctgacga ctgacactgg aaaccacgat ccccgcggca aataacagca cgccagcatg
    10921 agctaccagc gccggaagct gcacgcgttt gccagagaca aacgtcgcca gcacaatcac
    10981 caccagcatc aacagaccaa acggtaacgt cgcgcagtta aaatacggcg cgccaacgga
    11041 gaggcgtccc cagcccagca ggccgtaaat catcggataa agcgttccca ccagcacgat
    11101 cagtagcact gcgcaaaaca gcaacagcgt agcgaggatt aacatttccc gcgataaccc
    11161 cgaaaaacgc accgccgggc caccgtcccg cgctcgccag ccatacagag ccaaagacgc
    11221 aaggctaatc agtgcaaaca ggctgaacaa cggcacggcg cggacgttat ccagtgcgaa
    11281 cgcatgaacc gaaaccagaa tgccagaacg gacaattaag gtgcccagca gcgacagcat
    11341 cagagtaact atcgccagca acagcgacca gtggcggaaa atcccccgct ggcgtgtcag
    11401 ggacaaactg tgcagcagcg cagtggcaga aagccagggt aataaagagg cgttttccac
    11461 cggatcccag aaccaccagc cgccccagcc gagttcgcag taggcccacc aggaaccgag
    11521 gatgatcccc gccgttaatg cactccagcc aggtaacgcc cagcgccagc aaattcgggc
    11581 gctggccgca tcaaactcgc cgcgcagtag actcgccagc gccacgctcg ccgctaccat
    11641 caaaccgcca tagccaaggc aaagcagcgg tggatgaaag ataagaccgg gatgttgcag
    11701 catcggattg agatcgcggc cttcgattgc tggtggaaat atccgcacga agggatcgga
    11761 ccacagcacc acaaacagta gcagtgcggc gagcatgaga gataaaacgg ctagcgtcag
    11821 cggaaatagc ggatcgcttt gctgccgata atgccaggca aacagcgcgc tccaggcgga
    11881 aagcagcagc acccaaagca gcagcgaacc ttcatgaccg ccccacaccg ccgccagttt
    11941 gagttcccac gacagcaggc tgtagctatg ttgggcgaca taaatgaccg agaaatcgct
    12001 gatgagaaaa caatacgtca gtacgccaaa ggcgagcagc aggagcgcga actgcgccag
    12061 aatgccgatg caagtgagtc gcatcatggc aggccagcgc aaccgcactc ccgcaaaggc
    12121 cgtcaacggg gtcaacacgt tgaccccgag acttaacaac aacgccagaa aaccgacttc
    12181 aggaaggaat acgtccagaa accacctctt ttctggctta cgccaccgtc aactgtccgg
    12241 cgtagagaat gaaaaagcgc agcatcaaca cgccgaccag actggcccca cacgccgcca
    12301 gcacggcagg aatgccggaa ctgcgattga cccacggttt gagcaacatt ggcacaatca
    12361 gccccagccc ggcgacacca agccagaacc accacgtcca gaaaccgcca cctaatgccg
    12421 ccaccagcgc acgaacttta ccgtcatcgc ccagcgccag accgacaaaa aacgccacca
    12481 gcaggaagat ttcaccccat accacgggga tttccatccg gtgaacaaag tgcgcttccg
    12541 tggaatgcgg gttactgcgt tggcgtatcg ccatggcgat cagcgccacc gccgcaccgg
    12601 acgagatgcc ggagaagagg agcagcaccg gcaggctcgg gttattgagg aacggatacg
    12661 atttcagcgc cgacagcaga aagccggtat aagcccccag cagcaccgcc aacaccagca
    12721 tcagcgtttc cagtccgcga tgaacgggcg tcagcaggct cagaaccttt tgcacgatcc
    12781 ccagcttcgg caaccagcgt tgttgcaggg caagcaaatc atgttcaaag attttcgcca
    12841 gccacagcac cagcaccacc atgtagagct gaaacagcat cacccccatc gacatcaccg
    12901 aggtaaaact gtagtggaac atcagcttcc agaaggtcca cggtcttgtc aggtggaaga
    12961 cgaggatcaa cagaccgagg atcaccgcgc ccggcccgac aatcagcgtg gtgcgcagca
    13021 acgtactgtc tgcaccgccc gcctgcgggt agaagcgacg taacagcacg gccagcgtca
    13081 ccagaccggc agaaatacca atcaaaaaca ggtagatggc aatcggccag tcccacacca
    13141 gcgattcaaa atgaaatgcg gaagtctgcg tcattggctc acctccccgt atttaaacgg
    13201 tacgcggtac agcttcggtt tggttcccag cgccagcttg tagcggtaag taggcttctg
    13261 gcgcagcagt tgcgaaatct cactgttagg atcgtccaga ttgccaaacg tcagcgcctt
    13321 cgtcgggcaa gcttcaacgc acgcgggaag cttaccggcc tgcaaattgg tcttacgaca
    13381 gaaatcgcat ttgtccgccg tcttcgtgac cggatggata aagcgcacgc ggtacggaca
    13441 ggcggcgatg cagtactgac aaccgacgca gagatcagga ttaacatcga caatcccgct
    13501 agcggcatca cgaaaagacg caccggtcgg gcagacgtca acgcacggca catgatcgca
    13561 gtgctggcaa gacttacgga agaagcgata tttcacatca ggaaattcgc cctgcggctc
    13621 gctacgaata atcgtcaggc gcgagacgcc ttccggcacc ttgttcactt cccgacaagc
    13681 atccatacag gcggtacagc cgatgcataa cgactcgtca tgcaccatgc cgtaacgcac
    13741 cccattgata ttcaacgttt tcgccaccac gcgccctgcg gtgccgctga cggctgccag
    13801 cacgccgacg ccggtgagaa actggcgacg agaccaggtc atggctgctc cttaagcaac
    13861 ggaacagacg ccgggttaaa gttcggattg gtgcgctgat cgctgtggca atcgacgcaa
    13921 atcttaatcc gtcctttgtc acttaacgtc tgcatcgtat cttgctgtgg atgcagggaa
    13981 tggcagctgg cgcacgccac tttggttacg tggacatcgt gcggccagaa cgctttttgc
    14041 aactgttccg gcagatgaca ggacatacag acgctgttct gctctccgac cttatacatc
    14101 ggctcgttaa agcgcatcac atctttcacc ccttcgcggt gttgcggtga tgcttggccg
    14161 tgacagttgg tgcaggtgac cggcaatttg ttattcgggt tgataacgga agcatgtttg
    14221 ccatgcatac cttcggtatc tggtttatga cagtccagac aggcggcatc cgggttacgc
    14281 tgctgggtaa cttcgtagcg atcaccagat gcctgcaccg caggggtagc ggtaattccg
    14341 ttcaggctcc acaacaggcc cgacgccagc accccagcag ttaataacga acgtaatacg
    14401 ctcatattca ctccattgtg ttttccgccc cttgcgaggc ggaacggggt tattggctta
    14461 acagaccgtt tttacgtgcc tgctcttccc actgcgggat caccgttttg atgaagtcct
    14521 gcttctcggc cttgatttgt tccatgttca ggccaatggc ctgctgggct ttctctttgg
    14581 tcgagatatc cgggatctgg atttcatggg tgataccttt ggtcgccagc aggcgcgcca
    14641 gtttggtgcg cgcatccgcc gctttatcca tcgccgtacc gagcatccgt agaccttctt
    14701 ccggtgcgtg catatgaata ccgtgggaag cgatcgccag atcccaacgc cactgggcat
    14761 gacggatatc gtcctgaatc ggcttcattt cggcttccgt cgcgcctgca tccagcgccg
    14821 ctttcgcttc gaagtgagcg tgaaccagtt gatcttcaac cttgattttc aggtcgttaa
    14881 tcgattgctt acgttccgcg accacttttt gcaaggcagc tttatcctgg gtatggcagt
    14941 tcgcacaagt ctgggcgaag ttatcaaacg gattaccaat tttatggtcg gtgtagagtt
    15001 tgccttcggc gttctgcact tttggcatat ggcagtcgat acaggtcacg ttgtttttac
    15061 cgtgaatgcc cgctgtccag gtttcatatt ccgggtgctg cgctttcagc attggcgttt
    15121 tcgacaggga gttagtccag tcagagaagg caattttgtc gtaatactgc tccatatttt
    15181 cgactttcat gccgtcatcc cacgggaatt taaccgcttt gtttttgccg tcgaagtaat
    15241 actccacatg gcactgaccg caaaccatcg attgctggtc gaaacgtccg gctttctcaa
    15301 aaggtttacc aatggcttcc atcgcgcgag ccgcatacgg acgggaaagg gttaactccg
    15361 gtttgccttt ggcgaactct ggtgaggcgg tgttatggca gtcggcacaa cctaagttgt
    15421 tgacgatttc cgggccgccg cgcgcccatt taccgtggaa gtagccatct tcgccgtctt
    15481 tctggatcag acgcgccaca tccgggcttt tacaactcca gcacgccatc ggtagcggac
    15541 catcttcagc gtttttcggc gcaccggtac gcagggtttc acgcacatcg gtcacagcaa
    15601 aggcatgtcc acgaggcttg ttgtaatcgc gcgagaaggg ataccccgcc cacaggatca
    15661 ccagccgtgg atcttccgcc agggcgtcaa cacgctctga ctgttccgag gtggctttcc
    15721 aggagagata ttgatcgtga tgctgcgggg caaaggtttc attcttcgct tccacagtta
    15781 caggttttgc gggagctgcc gtttgttcag cgtgaacaga agtgaaaaag aaaaaaggaa
    15841 tcaataagct gaagatacgg cgtgcgttta tttttatcct tgtcataggg gcttcatccg
    15901 aattgcgcca ttgttgcaat ggcggttttt attgtttttc acgacagtaa ccgcacctac
    15961 actgtcatga cattgctcac ccctatgtgt aacaaataac cacactgtga atgttgtctt
    16021 taatcaattg taagtgcatg tgaaatacca ctttagagtt agtcagtatc ttcctctttt
    16081 tcaacagcat gcataactgc atgttcctca aagaattaat caacttttgt tggtgacctt
    16141 caaaaattac cctgccgttt atttgcacca ttctactttt gcgtgatctg tcgcccaaat
    16201 actaaacaaa actgccaata cccctacatt taacgcttat gccacatatt attaacatcc
    16261 tacaaggaga acaaaagcat gagccaaatt cacaaacaca ccattcctgc caacatcgca
    16321 gaccgttgcc tgataaaccc tcagcagtac gaggcgatgt atcaacaatc tattaacgta
    16381 cctgatacct tctggggcga acagggaaaa attctcgact ggatcaaacc gtaccagaag
    16441 gtgaaaaata cctcctttgc ccccggtaat gtgtccatta aatggtacga ggacggcacg
    16501 ctgaatctgg cggcaaactg ccttgaccgc catctgcaag aaaacggcga tcgcaccgcc
    16561 atcatctggg aaggcgacga cgccagccag agcaaacata tcagctataa agagctgcac
    16621 cgcgacgtct gccgcttcgc caataccctg ctcgagctgg gcattaaaaa aggtgatgtg
    16681 gtggcgattt atatgccgat ggtgccggaa gccgcggttg cgatgctggc ctgcgcccgc
    16741 attggcgcga tgcattcggt gattttcggc ggcttctcgc cggaagccgt tgccgggcgc
    16801 attattgatt ccaactcacg actggtgatc acttccgacg aaggcgtgcg cgccgggcgt
    16861 agtattccgc tgaagaaaaa cgttgatgac gcactaaaaa acccgaacgt caccagcgta
    16921 gagcatgtgg tggtactgaa gcgtactggc ggaaacatcg actggcagga agggcgcgac
    16981 ctgtggtggc acgacctggt tgagcaagcc agcgatcagc accaggcgga agagatgaac
    17041 gccgaagatc cgctgtttat tctctatacc tccggttcta ccggaaaacc aaaaggcgta
    17101 ctgcacacta ccggcggtta tctggtgtac gcggcgctga cctttaaata tgtctttgat
    17161 tatcatccgg gtgatatcta ctggtgcacc gccgatgtgg gctgggttac cggacacagt
    17221 tatttgctgt acggcccgct ggcctgcggc gcgaccacgc tgatgtttga aggcgtaccg
    17281 aactggccga cgcctgcccg tatggcgcag gtggtggaca agcatcaggt caatattctc
    17341 tataccgcac ccacggcgat ccgcgcgctg atggcggaag gcgataaagc gattgaaggc
    17401 accgaccgtt cgtcgctacg cattctcggt tccgtgggcg agccaattaa cccggaagcg
    17461 tgggagtggt actggaaaaa aatcggcaac gagaaatgtc cggtggtcga tacctggtgg
    17521 cagaccgaaa ccggcggttt catgatcacc ccgctgcctg gcgctaccga gctgaaagcc
    17581 ggttcggcaa cacgtccgtt cttcggcgtg caaccggcgc tggtcgataa cgaaggtaac
    17641 ccgctggaag gggctaccga aggtagcctg gtgatcaccg actcctggcc tggtcaggcg
    17701 cgtacgctgt ttggcgatca cgaacgtttt gagcagacct atttttccac cttcaaaaat
    17761 atgtatttca gcggcgacgg cgcgcgtcgc gatgaagatg gctattactg gatcaccggg
    17821 cgtgtggacg acgtgctgaa cgtctccggt caccgtctgg ggacggcaga gatagagtcg
    17881 gcgctggtgg cgcatccgaa gattgccgaa gccgccgtag taggtattcc gcacaatatt
    17941 aaaggtcagg cgatctacgc ctatgtcacg cttaatcacg gggaggaacc gtcaccagaa
    18001 ctgtacgcag aagtccgcaa ctgggtgcgt aaagagattg gcccgctggc gacgccagac
    18061 gtgctgcact ggaccgactc cctgcctaaa acccgctccg gcaaaattat gcgccgtatt
    18121 ctgcgcaaaa ttgcggcggg cgataccagc aacctgggcg atacctcgac gcttgccgat
    18181 cctggcgtgg tcgagaagct gcttgaagag aagcaggcta tcgcgatgcc atcgtaaccc
    18241 acaattgccg gatgcgaatc ggtaacggtt tgtaggcctg ataagacgcg acagcgtcgc
    18301 atcaggcatt gattgccgga tgcggcgtaa acgccttatc cggcctacat tcggcaaggg
    18361 ttacccgagc gttaaccttc tcccataagg gagcgggaat taaaacaatc cctacattac
    18421 ctctggagaa tctgtgatga atggtacgat ttatcagcgg atagaagaca atgcgcattt
    18481 cagggagtta gtcgaaaaac ggcaacggtt tgccaccatc ctgtcgatta ttatgctggc
    18541 agtttatatc ggctttattt tactgatcgc cttcgcgccc ggctggctgg gcacgccgct
    18601 gaatccgaac accagcgtca cacgcggtat tccaattggt gttggagtga ttgtgatctc
    18661 ctttgttctc accggtatct acatctggcg ggcgaacggc gaattcgacc gtcttaataa
    18721 tgaagtcctg catgaggtac aagcatcatg aaaagagttc tgacggcgct tgccgccaca
    18781 ctccctttcg cagctaacgc cgcggatgct attagcgggg ccgtagagcg ccagccaacg
    18841 aactggcagg cgattattat gttcctgatt ttcgtcgtgt ttacgctcgg cattacctac
    18901 tgggcatcaa aacgcgtacg ttctcgtagc gactactaca ccgcaggcgg caatatcact
    18961 ggcttccaga acgggctggc gattgccggg gactatatgt ccgccgcctc attcttgggg
    19021 atctccgcgc tggtgtttac ctccggctat gacgggctga tctactcgtt gggcttcctg
    19081 gtgggctggc caatcattct gtttctgatt gccgaacgtc tgcgtaacct gggacgctac
    19141 acctctgccg atgtggcctc ttatcgtctg aaacaagggc cgattcgtat tctttcggcc
    19201 tgtggttctc tggtagtggt ggcgctttac cttatcgctc agatggtagg cgcaggtaaa
    19261 ctgatcgagc tgctgtttgg ccttaactat cacattgcgg tggtgctggt cggcgtgctg
    19321 atgatgatgt acgtcctgtt cggcggcatg ctggcgacca cctgggtaca aattatcaaa
    19381 gctgtgctgt tgctgttcgg tgccagcttt atggccttta tggtgatgaa acacgtcggc
    19441 tttagcttca acaatctgtt cagcgaagcg atggcggtac acccgaaagg tgtcgacatc
    19501 atgaaaccgg gcggactggt gaaagatccg atctccgcgc tctctctggg gctgggactg
    19561 atgtttggta cggcgggctt gccgcacatt ctgatgcgct tctttacagt cagcgatgcc
    19621 cgcgaagcac gtaagagcgt gttctacgcc accggattta tgggctactt ctatattctg
    19681 acctttatta tcggcttcgg cgcgatcatg cttgttggtg cgaatccgga atataaagac
    19741 gcggcgggcc atctgattgg tggtaacaac atggcggccg ttcacctggc gaatgcagtg
    19801 ggcggcaacc tattcctcgg ttttatttca gcggttgctt tcgccactat cctcgcggtg
    19861 gttgcggatc tgacactggc gggcgcatcg gcggtttcgc atgacttgta cgctaacgtc
    19921 ttcaaaaaag gcgcgaccga acgtgaagag ctgcgggtat caaaaatcac cgtactgatc
    19981 ctcggcgtga ttgcgattat cctcggcgtg ctgtttgaga atcagaacat cgcctttatg
    20041 gtaggccttg cgtttgccat cgcggcgagc tgtaacttcc cgatcattct gctttccatg
    20101 tactggtcga aactgactac ccgtggcgcg atgctgggcg gctggctggg gctgattacc
    20161 gcagtggtgc tgatgatcct cggcccgact atttgggtac agatcctcgg tcacgaaaaa
    20221 gccatcttcc cgtatgaata cccggcgctg ttctctatca gcgtggcatt cctcggcatc
    20281 tggttcttct cggcaaccga taactcagcg gaaggggcgc gcgagcgtga actgttccgc
    20341 gcgcagttta tccgctccca gaccggcttt ggcgttgagc aaggccgcgc acattaatct
    20401 ctccccttcc ccggtcgcct gaccggggaa tacgcttcct ctccaacatg catcaccttt
    20461 tcccataata ttaaacaaat aaactcatta aaaaatgagc gatttttgac agtcgtagaa
    20521 aatgataatg cagagaatat gccttttctt tcttgttaat tataaggata ttttatgcgc
    20581 tataatggtt taaataatat gtttttccct ctttgccaga ttaacgataa ccactctatc
    20641 acaagttcat cacatacaaa gaaaacaaaa tcagataatt acagcaaaca tcataaaaac
    20701 acgttaattg acaataaagc cctctctctt ttcaaaatag atgatcatga aaaagtgata
    20761 ggcttgattc agaaaatgaa aagaatttat gatagtttac catcaggaaa aatcacgaaa
    20821 gaaacggaca ggaaaataca taaatatttt atagatatag cgttatatgc aaataataaa
    20881 tgtgacgata gaattacgag aagagtttac cttagtaaag aaaaggatgt atccattaag
    20941 gtggtatatt ttataaataa tgtcgccgtc cataataata ctatcgaaat tccacagata
    21001 gtaaatggtg gttacgattt ttcacacctt agcctgaagg gtatcgtgat taaagatgaa
    21061 gatttatcca attcgaattt tgcaggttgc agactacaaa acgctatttt ccaggactgt
    21121 aatatgtata aaacgaattt ttattacgcc ataatggaaa aaatactttt tgataattgt
    21181 attctcgatg actcaaattt cgctcagata aaaatggccg acggaactct aaatgcatgc
    21241 tccgctatgc atgttcaatt ctacaatgca gcaatgaata gagccaatat taaaaatacc
    21301 ttccttgact attcaaattt ttatatggcg tacatggctg aggtaaatct ttataaagta
    21361 atagcgccat atgttaattt atttaaagcc gaccttagtt tctctaaact cgatttaatt
    21421 aactttgaac atgctgatct gtctcgcgtc aatctgaaca aagcaatcct ccagaatata
    21481 aacttaattg atagcaaact cttttgtacg tggctgacaa atacattcct cgaaatggtt
    21541 atatgtaccg gctctaatat ggctaatgtt aattttaata atgccaattt aagcaactgc
    21601 catttcaact gttctatttt aacaaaagcc tgtatgttta atacccgtct ctatcgggtt
    21661 aatttcgatg aggctagcgt ccagggaatg ggcatttcca ttctccgtgg ggaggaaaat
    21721 atccccattg atagtgatac cctggtaaca ctacagaaat tctttgaaga agattgtacc
    21781 tctcatactg gcatgtcaca aactgaggat aatattaatg cagtcgctat gaagattact
    21841 gcagatatta tgcaacacgc agattgaggt taagtcgaag aacagccaat gaattgactt
    21901 tacaagagag ataacttatt tgttttagaa tcatgaatgt tcgcccgagc taacctgtta
    21961 aaggtgcaaa agattgcacc tttttcagat tacctcgggc ctggttacgt cgctttcggc
    22021 ttactgattt tcctcaatta gcaacgcttc aagcaaatcg agatcgtgca gtaatttttg
    22081 cagcgtttcg ttgctgatct cccgcgtggc gcgcaggtgg tacagttcag cacgttcaga
    22141 acgcaatgcc gccagacgga agcgacgctc aaggttctct tcctgcacgg aactttcaac
    22201 gtcgttacgt ccatcggcgc gacgacgcag gttaccaatg acgcgagaac tgacctccgt
    22261 aagcaactgg ttatcgatat tctcttcggt atccgccgcc agacgctcct ccattttctg
    22321 gatcgccaca atcgccactt ctgccgttgc cgctcgcgca atacgttctt ctttcaattg
    22381 ttgcgaatgg tccgcgactt caatgtgttg tagcagaata ggcaacatca ccacgccgac
    22441 aaacagcgag aagagaatga cgccagccgc caggaacacc agctcatagc gcgccgggaa
    22501 gacgttacca tccggcaaga gcagcgggat tgagagcaca ccggccagag tgatcgcccc
    22561 acgcacccca gcgaaagacg cgatcaggat ttctcgtgtg gtccacgaac caaactccat
    22621 cggcttcttc ttcaggaagc ggttgctgaa ctttttcatc gtccacagcc agccgaaacg
    22681 gaccagcatc agcgccgcat atatcagaat aatatcggta aacagcatcc agatttcgac
    22741 gttagggtcg atttctgccg ccatcagcga cgtctccaga atacccggca gttgcagacc
    22801 taacagcagg aacaccatgc cgttaaagac aaattccagc atcgcccagg tgctgtttgc
    22861 gcgcaggcgc attgccagtg gcgcacggcg catcacaccg gagcgggtga tggtcatccc
    22921 ggcggcaacc gcagcgagga tgcccgaaac gccaatatgt tcggcaatca gataagaagc
    22981 gaatggcagc aagaacagca gaacgatctg cgtcgcgggt tcatcaccgc cccagcggct
    23041 gaggaatcgc agcgagcgac catacagcca gctcaccaca aaaccggcaa gaataccgcc
    23101 aatggctact ttcataaatt cgaccgtcgc accgccgaca gtgaagatca tcgtccccat
    23161 cgccactgcc acggcaaact tcaacgacac caggccggaa gcgtcattca tcaacgcttc
    23221 gccctgcaaa atgcccatga tttttttcgg gatgcgccct tcgccaacaa tcccggagag
    23281 cgccacagca tcggtcggag aaagtaccgc cgccagcgca aaggcgggga tcagcggaat
    23341 gcccggcacc acccagtaaa taaggaagcc aatgccgacc acggtgacca ccaccagcgc
    23401 cagcgcgagg ccgaaaatct ctcgaccatg ttcgagaaat tcacgggtcg gcgttttcca
    23461 gccatcagcg aacagcaacg gcgggataaa caagactaaa aagagttcag gatcaaactc
    23521 cacatgcaaa ccaaacgtcg gccacgccag tagcgcaccg atggcgattt gcataagcgg
    23581 aagcgggatc tgaaagggca tgacacgagt gaccaccccg gacagcgaga ccacgagggt
    23641 cattatcagt atggtgaaga agatttccat gcgttccctg tttgcgatgt tttttatgat
    23701 ctgagaaaga cgttatgcct gaattctcat tttttcagaa tatcgcaaaa gctcacacaa
    23761 tgtgcggaga aaataaaaac gggcagccat tgccacccgt tttcagaatc cgatgaaaga
    23821 ttagatagcc cagccacccg catagaaggt caccagcgcc acggcgataa cgaccgtacc
    23881 gatgttcaac ttgcgccatt caccggaaac cagacgacca atcaccagag tcgcgaagcc
    23941 gatcatgatg cctgttacga tgttacaggt cagcacgatg aataccgccg taaccagacc
    24001 cgccatcgca tcaacaaaat cagcaaagtc gattttcgcc acgttgctca gcatcagcag
    24061 gccaacgtac atcagcgccg gagccgttgc ataccccgga acgaggtaag aaagcggaga
    24121 gaggaacaga atcagcagga acagcacgcc aacggtgata gccgtcaacc cggttttacc
    24181 gcccgccgcc gtacccgccg cagactcgat gtataccgct gccggagctg cacccaccag
    24241 gccagagaaa acgctgctca tggagtcagt ggtcagtgct ttcccgccgt cgatgatctg
    24301 cccatcttta tccagcaggt tcgcctggcc ggcgacagcg cggatagttc cggtggcatc
    24361 aaatactgcc gtcatcacca gcgccagaac gcttggcagg actacaggat tcagcgcgcc
    24421 cataatgtcc aggctgccaa tcagtgaatt gccgttttca tcgctcaatg aaggcatggc
    24481 gaaaacgccg gagaaatgga cgttaggatc gaagatcaaa ccgacaattg agataccgat
    24541 aatggtcagc agaatgccac cagggacttt cagtttttcc aagccgatga tcaccgccag
    24601 acctaccagt gacataatca ccgggaaggt cgcgaaatca cccagcgcaa cgggcagacc
    24661 atcaagcggg tttttaatca ccagaccgac accattagcg gcaatgagca gcaggaacag
    24721 accgataccg atccccgtgc cgtgcgccac accgtgcggc aagttgcgca aaatccagct
    24781 acggataccc gtggcagaaa ttaccgtaaa cagcacaccc atcaggaaca cggcacccag
    24841 cgcgacaggt acgctaatat gttgccccag caccaggctg aatgcggtaa acgccgtcag
    24901 ggagatggcg caaccaatcg ccaacggcag attagcccac agacccatca cgatagaacc
    24961 gagtccggca accagacagg ttgcaacgaa aactgccgca ggcgggaagc ctgctttacc
    25021 caacatacct ggaacgacga tgaccgagta gaccatcgcc agaaacgttg ttaacccggc
    25081 aaccacttcc tgacggacag tgcttccacg ttgtgaaatt ttaaaccagg cgtcgagtga
    25141 accgccggta cgcgctgatg gcgtagacat aagaaaacat cccctgagag tttaattttc
    25201 gtcaagatgc gtggcggaca atcccctgcc agcgaaacgt tatcttcctg ctccaggttt
    25261 gcggcaattt ggtgcgtcgc aaaaaagcaa acgtttagct ccgcgcatac aaaacgggtg
    25321 gtaaaaaaaa gcaaacgatt atccggcgat catagcggct ttttcaaccg aagtttgctg
    25381 cttttttctc ttttattgtc ttgacgatga aaaaccaggc aattgatgcg caaacgttat
    25441 cgtcgatgtg caagattgca gcgttatcga gccgtatacc gttctggatt gcgataaaaa
    25501 atcgtctgag atgacaaaaa tattgcggct aaaaagtata cttttacact ggcgaagaaa
    25561 cgtcggtgac atacaattaa agcatcaaca ccaaccggaa cctccaccac gtgctcgaat
    25621 gaggtgtgtt gacgtcgggg gaaaccctcc tgtgttccag cgggatagag agaaagacaa
    25681 agaccggaaa acaaactaaa gcgcccttgt ggcgctttag ttttgtgttt gcggataatt
    25741 ccaaaagagc tcgatcagtc ccctcgcccc ttcggggaga gggttagggt taggggaaca
    25801 ggcagcacca gtgcaaatat ttaccctcac cccggcccca tagttaacga tgagtcagct
    25861 tgtccttgtg gcgctttagt tttgttcatc ttccagcaag cgtgcgccgg taccttcttc
    25921 tcctaagcgg tcgcccgggt tacgcaacgg gcaatcactg cgcgaaaggc agccacaacc
    25981 aatacatccg tccagttcgt cacgcagcgc cactaaggta tgaatgcgcc gatccaactc
    26041 ttctcgccat tgggacgaaa gctgtttcca ctctttcgca cttaacgtat gcccttctgg
    26101 caacacgcca aacgcttcac caatggtcgc cagcggaatg ccaatacgct gagcgatttt
    26161 gataattgcg acatatcgca acacatcacg tttatatcgc cgctgattgc cgctgttacg
    26221 gatactagta atcaaccctt tgctttcata gaaatgcagc gccgataccg ccacaccgct
    26281 gcgtttcgcc acttcgccag gggttagcag cgctttaatg cggggtaatt tcttttccat
    26341 aaatcgcttt acctcaagtt aacttgagga attatactcc ccaacagatg aattaacgaa
    26401 ctgaacactg aaaagaggca gatttatgtc ccatcagaaa attattcagg atcttatcgc
    26461 atggattgac gtgcatattg accagccgct taacattgat gtcgtcgcaa aaaaatcagg
    26521 ctattcaaag tggtacttgc aacggatgtt ccgcacggtg acgcatcaga cgcttggcga
    26581 ttacattcgc caacgccgcc tgttactggc cgccgttgag ttgcgcacca ccgagcgtcc
    26641 gatttttgat atcgcaatgg acctgggtta tgtctcgcag cagaccttct cccgcgtttt
    26701 ccgtcggcag tttgatcgca ctcccagcga ttatcgccac cgcctgtagt tttattgccc
    26761 gcgcgttaac tcccgcgcgg gtaattgctc catccattgc ataaacacct gcggcggcat
    26821 cgccttcgca aaaaaccatc cctggcaata gcgcacgccg cgtttgcgca gccagttaac
    26881 ctgctcctca gtttcgacgc cttcagcgat cgtttttaac cccaggctgt gcgccagctc
    26941 gatgatgtgt tccgcaatca aatgactggt tttgtgggtg gtcagcgttt caacaaacga
    27001 tttatcgatt ttcaaaatat cgacattcaa tgatttaagg ttatgcaagt tagagtagcc
    27061 aataccaaaa tcatcaattg ccacttcgta accagcctgg cggaaagcca gaattaatcg
    27121 gcgtcatttt ttcgacatca agaaatgcat gctcagtcac ttcaaattta atttgctgcg
    27181 gacgcaccgc gtattgctct gttttctgat tgattcgcgc tatcaaccgt gacgtatgaa
    27241 aatcggaggc cgacaggtta atagaaacat agcgatcggc atgtgttgcc aggtacgcgc
    27301 ccagatcgcg gaagacatta tcaataacat aatcagttat ctgttctatc atcccctcct
    27361 tttctgccag cggaataaac tctgccggat tcattatttg cccctgctca ccaggccaac
    27421 gtaacaacgc ttcagcgccg atacatttt
//
LOCUS       DAEEGA010000055        26563 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEEGA010000055 DAEEGA010000000
VERSION     DAEEGA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26563
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.055"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            348..641
                     /locus_tag="KLH46_002473"
     CDS             348..641
                     /locus_tag="KLH46_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBE0379120.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="KLH46_002474"
     CDS             713..1372
                     /locus_tag="KLH46_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBE0379121.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="KLH46_002475"
     CDS             1464..1910
                     /locus_tag="KLH46_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBE0379122.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="KLH46_002476"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="KLH46_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="KLH46_002477"
     CDS             3390..3809
                     /locus_tag="KLH46_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBE0379123.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="KLH46_002478"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="KLH46_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBE0379124.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="KLH46_002479"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="KLH46_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBE0379125.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="KLH46_002480"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="KLH46_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBE0379126.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHSIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="KLH46_002481"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="KLH46_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBE0379127.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9864)
                     /locus_tag="KLH46_002482"
     CDS             complement(8332..9864)
                     /locus_tag="KLH46_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBE0379128.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10194..11492
                     /gene="dadA"
                     /locus_tag="KLH46_002483"
     CDS             10194..11492
                     /gene="dadA"
                     /locus_tag="KLH46_002483"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBE0379129.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11502..12572
                     /gene="dadX"
                     /locus_tag="KLH46_002484"
     CDS             11502..12572
                     /gene="dadX"
                     /locus_tag="KLH46_002484"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBE0379130.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12740..14154)
                     /locus_tag="KLH46_002485"
                     /pseudo
     CDS             complement(12740..14154)
                     /locus_tag="KLH46_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14765..16501)
                     /locus_tag="KLH46_002486"
     CDS             complement(14765..16501)
                     /locus_tag="KLH46_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBE0379131.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="KLH46_002487"
     CDS             complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="KLH46_002487"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBE0379132.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17610..18221
                     /gene="emtA"
                     /locus_tag="KLH46_002488"
     CDS             17610..18221
                     /gene="emtA"
                     /locus_tag="KLH46_002488"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBE0379133.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18223..18957)
                     /locus_tag="KLH46_002489"
     CDS             complement(18223..18957)
                     /locus_tag="KLH46_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBE0379134.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19158..19412
                     /locus_tag="KLH46_002490"
     CDS             19158..19412
                     /locus_tag="KLH46_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBE0379135.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19462..20922)
                     /locus_tag="KLH46_002491"
     CDS             complement(19462..20922)
                     /locus_tag="KLH46_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBE0379136.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20859..21416)
                     /locus_tag="KLH46_002492"
     CDS             complement(20859..21416)
                     /locus_tag="KLH46_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HBE0379137.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21442..22296)
                     /locus_tag="KLH46_002493"
     CDS             complement(21442..22296)
                     /locus_tag="KLH46_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HBE0379138.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22296..23105)
                     /locus_tag="KLH46_002494"
     CDS             complement(22296..23105)
                     /locus_tag="KLH46_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBE0379139.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23115..23873)
                     /locus_tag="KLH46_002495"
     CDS             complement(23115..23873)
                     /locus_tag="KLH46_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0379140.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQMALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23870..24850)
                     /locus_tag="KLH46_002496"
     CDS             complement(23870..24850)
                     /locus_tag="KLH46_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBE0379141.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24850..25872)
                     /locus_tag="KLH46_002497"
     CDS             complement(24850..25872)
                     /locus_tag="KLH46_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HBE0379142.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26221..>26563
                     /locus_tag="KLH46_002498"
     CDS             26221..>26563
                     /locus_tag="KLH46_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379143.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6684 a         6473 c         6848 g         6558 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgtga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagcgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa taggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagaatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca accccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     8941 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     9001 gaaggtcgca cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     9061 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     9121 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     9181 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     9241 atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg
     9301 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     9361 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     9421 atcgacaatc gaactggcgt cggtccaact gcggaataag caattgtttt taaagaaaga
     9481 gttatgtcca tagcaggcgt gtgccatcac cagcgcctgc atagtaatgg tgttctcttc
     9541 catcaagtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gcccttgctg
     9601 accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata
     9661 gttaattggc atgccgacgc tggaataggc atccatcatc tgttctgagg ttatcacttc
     9721 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     9781 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     9841 attcatagaa tcgatcgtcg ccatacacgc acctcattgt tgtcggcgct ctctgtgtgg
     9901 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     9961 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
    10021 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
    10081 tgtgagtcag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
    10141 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
    10201 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
    10261 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga
    10321 atgccgggca aatctccccc ggatatgctg caccgtgggc agcaccaggt gtgcctttaa
    10381 aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc
    10441 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
    10501 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
    10561 gcgccgaaac caatattcaa tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
    10621 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
    10681 cgtatcagct gctggaatcc agccgcctgg cggaagtgga tcccgcgctg gcagaagtag
    10741 cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac tgccagctgt
    10801 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
    10861 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagttt ggcgatgaag
    10921 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
    10981 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
    11041 aggaagatgg tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc
    11101 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
    11161 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
    11221 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
    11281 cggttgtcgg gagtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
    11341 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
    11401 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
    11461 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
    11521 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
    11581 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
    11641 ggagcgcact cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
    11701 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
    11761 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
    11821 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
    11881 ggatgaatcg gttaggcttc cagtccgatc gcgtgcttac cgtctggcag cagttgcggg
    11941 caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgctgaagcg gaacatcctg
    12001 atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc
    12061 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
    12121 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
    12181 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
    12241 cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
    12301 ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc gcctaccggt actcctgttt
    12361 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctggcggttg
    12421 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
    12481 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcgc
    12541 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
    12601 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
    12661 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
    12721 aactcgccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcggagtcga
    12781 cttcagataa atcacgcttc acccttgatg cagattcggg ttcatgttca gattgttgat
    12841 taacaggctg cgagtcctgt tgtctttcca gactcaacag gcccaactgt tgatccagga
    12901 gctccaggtc ctcctgtacc ttcacgcgct ccaggagttc cgggtcatcc aggaactcca
    12961 gatccataag gtcctccagc tcctcgagtc cttccaggtc aacctagcct tccttgtgca
    13021 aaaagtccaa aaggtccttc aggccctgct gttttttcag gccctcgacg gctttgagca
    13081 gcgtcggtaa attgtctttg tcccacgggt taccattaca gtcaaacacc ttcaggctct
    13141 ggggctgcgg cacctgtacc gttttaaact tgttttctcc acagtagtaa attattagtt
    13201 tgggcggcag ccgtggtgaa gagatcaaat cgttactaga acagtcaaat actttcaggc
    13261 gagtcaagtt ctccagcatc tcatcaggta ttgagttaat attgttgccg gcacaggata
    13321 gtttatacag gtactggggc atcgacggcg gtaaaaattg caagaagttt tcagaacagt
    13381 taatcactct caggttagtc gacagctcag gcagccagat aatactgttt tcatagcact
    13441 tcagtaccat cagttttgag ggctgcacct tcggtaacaa gatcaagacg ttatctgtac
    13501 agtcaagcac cagcagccac tttggcatca ccttcggtaa cgcggtcaac acgtttttac
    13561 tgcagtaaaa ttcaatcaga tgtggaggca aattctcagg gagtgaagtc aatcccaggt
    13621 tgctaagatt gagcatactc cgtcttgtaa tgagacattc cttcattctg cgcaatgctt
    13681 ctgcccggtt ttcaatccgg cttgctggca ggttttcttc caggatattt tccttgattt
    13741 cttccagact ttctttcagg tcttcttggg gaatttctga ctgtttgttt tgctggtttt
    13801 cttccaggat ttcgtacagg tttttttgca ggttttcttg caaaccatcc tgctgtatag
    13861 cccattcgca ccatttggcg accatcatga actcgttatg ttgctgattc agacgagtcc
    13921 ccaacgtgat gatggtattg ttgtccacct gtatgttcac tttgcccgcg atactgaggt
    13981 caatgatctg ccagggggca tcaccccagc tgaatttttg cgggctgaga tacttcatgt
    14041 tctgcaaaat ttgattcttg agaagagccg ccctggcggg cgtcgtacgc ctgggttaac
    14101 ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct ttaaagctag ccataatgct
    14161 acgtttcctt gaaataaata tcctctggca gcgctggaga cgtgaatgtg gtcaccgcaa
    14221 aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt tttccctggt
    14281 cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc tctgacgtat
    14341 taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa ggaataacta
    14401 taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg tgtgggctat
    14461 gtcagtgcgg caggttgcgt gggggaatat gttcaggcga tgtcaggcag gagcccctga
    14521 tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa aaagccctta
    14581 cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa tggtttgtca
    14641 tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg aaatgacaag
    14701 tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag atgctgcatt
    14761 aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac acttcattat
    14821 cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca ccgacaaccg
    14881 gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta tcacgatatt
    14941 ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg ctggcttcga
    15001 gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg ctgaacagtt
    15061 taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg ccttcgcgca
    15121 gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct gcaatacgcg
    15181 tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta tcagcactca
    15241 attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca acgcgtgaca
    15301 ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac gagagtgatg
    15361 ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg
    15421 cattctccag ccccgccatc atcgggaaca cggctaggat gattggcacc gcgccgcgta
    15481 atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa gggagcaatc
    15541 cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa atgagcgccg
    15601 gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc aggaacatgg
    15661 cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag cggttgcgaa
    15721 tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca
    15781 gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataatc
    15841 cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga
    15901 tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa ctgacactgc
    15961 tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc attggatcat
    16021 tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca
    16081 gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataagcctt
    16141 caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa
    16201 gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag gaactggcct
    16261 gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt accatgtagg
    16321 cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc agcatgccga
    16381 tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa ctaagtaata
    16441 tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca
    16501 acggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga
    16561 gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg
    16621 ctaatcgtca actgagcgcc ttcccgggtg ttattcagaa tggcatgtgc gcccaacggc
    16681 agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg
    16741 cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattgggc
    16801 gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat tcccgcatga
    16861 tagagctgca acagcatacg ttcgacgcgg aaagggtgca cgttaatatc ttccagcacc
    16921 agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgca
    16981 agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca
    17041 atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt
    17101 tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccttgc
    17161 gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag taacgggtca
    17221 tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg
    17281 taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc aagggaattg
    17341 agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc
    17401 tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg
    17461 tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat ggcaattcct
    17521 tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac
    17581 ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg
    17641 tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg aacgcgaaag
    17701 ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg
    17761 gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg
    17821 cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg
    17881 atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccag
    17941 agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag
    18001 gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct aacggggcag
    18061 gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatt
    18121 tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc
    18181 gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc
    18241 cgctttttcc cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac
    18301 cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt
    18361 gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt gaaaaacacc
    18421 ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta attcggcagg
    18481 ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca agcggaaacg
    18541 taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga gtggggcgga
    18601 gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta cggtaataaa
    18661 tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc
    18721 accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga tgttgtcttc
    18781 ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat
    18841 cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca aatcgcgtaa
    18901 cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg
    18961 gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac aaaacttaat
    19021 acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac gcccagcatc
    19081 gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga ccgattcatc
    19141 gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca
    19201 ttatcggcaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc
    19261 tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc attgacggct
    19321 ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct atcctggtgc
    19381 tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag
    19441 atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga aattgcgacc
    19501 ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat tgaccgacgc
    19561 attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc
    19621 ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag
    19681 cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg tctgcgttgg
    19741 aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca gagcgtagct
    19801 caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta
    19861 atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt gcgagaggat
    19921 ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc gcgtgaaggc
    19981 accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgcg ggttaacaat
    20041 cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg tcggaaagcg
    20101 tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt atttccccat
    20161 cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc tgccatcttt
    20221 ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg ccacgacatc
    20281 gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata
    20341 gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac
    20401 ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc cggcaccgtc
    20461 gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt
    20521 gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca gggtaaaacg
    20581 gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact ctggccactg
    20641 ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt tttcaccatc
    20701 ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac cgagcttcac
    20761 aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc
    20821 attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg
    20881 caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg
    20941 actacggctc catccctggc gatatcccag acttgcttct tcagggtggt gatattaatg
    21001 gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga aacttcgact
    21061 gcccccagat tgttggtcag aatccgcgcc agatcgaggt tgccgtcata ggggacataa
    21121 atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg aacttttacc
    21181 tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac acttaatgcc
    21241 tgagcgacat tctgtttatc caaggccttc atggtgggtt gatcgagaac ggtggtcgtc
    21301 gtcgatgata ccggactgga ccatacggtc agcgtgtcgc tctcctgagc cgctacggcc
    21361 tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag tctcatagaa
    21421 taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga ctttaatgtc
    21481 agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta
    21541 atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt gaggagccag
    21601 cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt cgagctgaag
    21661 tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg cttcaacaac
    21721 aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc agccctgatt
    21781 gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac atccggcacg
    21841 atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg tgcccggaat
    21901 tgcttttcgg gtgcaggcga tattaccgtc aggataacgt tcacgaagca atgccagcat
    21961 ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca
    22021 tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac ccgcgttcgc
    22081 ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta acatcttaca
    22141 cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta tatagccagg
    22201 ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat cttccagcag
    22261 taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg cggtacgcta
    22321 tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt
    22381 tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag actccagctg
    22441 acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccacggata
    22501 aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa gatatagttg
    22561 ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg
    22621 ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgca
    22681 tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggcagg
    22741 tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc cttcagtgcg
    22801 gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaaccgta
    22861 gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat atcgaataag
    22921 gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct gtccgttggc
    22981 gaggcgcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg cgttttttct
    23041 gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat atcatcaatg
    23101 agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg gtcggcagaa
    23161 acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa gccttgtgtt
    23221 actcgtccat ctggctctac ttggataatg ctgtcggcaa tggcgttggc atggagcgga
    23281 tggtgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag cgtgtccaga
    23341 agttggatct gatgaccgaa atcgaggctg gaggcgggct catccagtaa caacaagcgc
    23401 ggttgttgga cgagtgcacg ggcgattaaa gccatctgac gttcaccgcc gctaagcgta
    23461 ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc cgtggctttc
    23521 aggcgttctt gcttaccggg aacggaaaaa gcgcctatgg tgggggccag gcccatcaac
    23581 accatatcca gcacactgaa agcaaaaata ccgtcgtgcg cctgcggcac ccaggcaata
    23641 gcctgtgcgc gctggcgatg gctgagtgtc tgcacagaga ggctatccag taggatctcg
    23701 ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt gccgcaacca
    23761 ttcgcgccaa gcagacaaca aattgttcct tgcgacaggg tgaaggacac gtcgcgtagc
    23821 actggatggc gggagtaacc cagagacgca tgacgaacgg caagcaaagt cactggcgac
    23881 ctccgcgtaa caacaaaagc aggaaaaacg gtgcgccaac aaaagcggtc agaatgccaa
    23941 gtgggatttc tgttgttcca atgctgcgcg ccagcgtatc ggtaagcagg agcagaatag
    24001 caccagtaca cattgccata ggcaacagct gctgatggtt atgaccggtc aacaagcggg
    24061 caatgtgcgg aacgaccaga ccgatccaac caattatgcc cgctattgct accgtgctgg
    24121 cggtgattag tgtggcgcag acaatcaatc caaagcgcag acgcgtcacg ttcagtccca
    24181 gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg agaaaaaaca
    24241 ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt aaggtcacgg
    24301 tggacagtcc ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa ggatcggcca
    24361 gcgttttaat tagcgatatc cccgctccgc acaacgttcc taacgcaatg cctaccagca
    24421 ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag acgcccgcga
    24481 ccaccatcag cccgccgcaa aatgcgtaca gctggatgta aacaatgggt aatccccaca
    24541 ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata tccggtgaaa
    24601 ctaagggatt acgcaacatt ccctgatagg tggttccggc tcctgccagc gctgcgccca
    24661 gtaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt tgttcctgca
    24721 cagactcatt ttcttgacga agaattagcg ccagcagttg ctgcatatcg agatgataag
    24781 ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata catccgttta
    24841 cgattctcat tagctcgcca ccaatttttg atagtcggca tcactgagtg aacaatgcca
    24901 gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg cgggatccag
    24961 ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg catcgagcca
    25021 gccaaagggc aagccactta aaaataagat gcgttgttcc gcaacggctt tcacgccctg
    25081 ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca gaataatatc
    25141 cggctgccag cgcaggagat tttccatgga aacctgtgtc aggccgtggc gatcggctat
    25201 ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg aaccctgtaa
    25261 gcccgtttcc agccctcggg gaccgcgcgc agcataaaag cggaggttag cggcgggtga
    25321 ggtggcgaat gcctgagctt caccaacgaa gtggctggcg agattggctt gttctgcggc
    25381 gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg attgcgccag
    25441 ttttccgtta agcaataacc aggggatttg tgtctgttcg ctaacctgcc gtgcctggga
    25501 gatcaaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta acgccataag
    25561 cccttccagc gatagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg gtaactggcg
    25621 aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg aaaaaccgac
    25681 cattttttct ggcgcgacag ccagcaagag caaatcggcg ggcgcgcctg cgctgacgat
    25741 gcgtgtaatg ttttgggctt cgggaagaga ttgcgaagca aaacgattaa ctgtttgtga
    25801 aaaggacggt agagactgca ggagcagggt agatgcaagt gcctgcgcaa acgtccgtct
    25861 ggttatgggc atggagaaca tcctgaaaag taaaatcgat atatactttc atatatatcg
    25921 ataaaaatca tgttcaatat caaagtttag ctattgaaaa catgaagatg tgacgacttc
    25981 cgcaagcctg ttgaccctct ctatcattaa aaatgtattt atcaggtggc taatattatt
    26041 taaatcattt aaggctaaat catttaaata tgaatgacat tattgttttt tatcgtccac
    26101 ggttacgcga cgttgaagtt acttgtttta taatgagcga tttttgatat ctcgccgaga
    26161 aaattatcag cattctctcg tttcagtgtc gtgaatatac tgttaacgga ggtgaaaaga
    26221 atgctggggg ttatacaaaa cctatgttgt tgtcgtccgg attcaccgct tgctgatagt
    26281 aagcagcgta accagaagtt accgcaacaa gactttgagg attttaagaa cgacgttatt
    26341 aatgtcatta acaacaatga agatatttat tttcttagag agagtaattt actgaatgcg
    26401 tttattcgga cgtgcaaaaa aagagatgat aacagttacg gaaatatgat atttacgctt
    26461 tattttgata acgatatcta tgggaacaga attatcaaag catcgctgaa cggcggagag
    26521 ttgcagttat taagcacagg gtgtagcaat gataacattc tcc
//
LOCUS       DAEEGA010000056        26076 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEEGA010000056 DAEEGA010000000
VERSION     DAEEGA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26076
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.056"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="KLH46_002499"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="KLH46_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBE0379144.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="KLH46_002500"
     CDS             complement(2138..2584)
                     /locus_tag="KLH46_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBE0379145.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="KLH46_002501"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="KLH46_002501"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBE0379146.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="KLH46_002502"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="KLH46_002502"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBE0379147.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="KLH46_002503"
     CDS             complement(5347..6009)
                     /locus_tag="KLH46_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBE0379148.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="KLH46_002504"
     CDS             6182..6595
                     /locus_tag="KLH46_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBE0379149.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="KLH46_002505"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="KLH46_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBE0379150.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="KLH46_002506"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="KLH46_002506"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBE0379151.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="KLH46_002507"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="KLH46_002507"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBE0379152.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="KLH46_002508"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="KLH46_002508"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBE0379153.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="KLH46_002509"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="KLH46_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBE0379154.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="KLH46_002510"
     tRNA            complement(9861..9948)
                     /locus_tag="KLH46_002510"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="KLH46_002511"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="KLH46_002511"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBE0379155.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="KLH46_002512"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="KLH46_002512"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBE0379156.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="KLH46_002513"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="KLH46_002513"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBE0379157.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="KLH46_002514"
     CDS             14005..14592
                     /locus_tag="KLH46_002514"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBE0379158.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="KLH46_002515"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="KLH46_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBE0379159.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="KLH46_002516"
     CDS             14984..15841
                     /locus_tag="KLH46_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBE0379160.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPM"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="KLH46_002517"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="KLH46_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBE0379161.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="KLH46_002518"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="KLH46_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBE0379162.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="KLH46_002519"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="KLH46_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBE0379163.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="KLH46_002520"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="KLH46_002520"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBE0379164.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22444)
                     /gene="etk"
                     /locus_tag="KLH46_002521"
     CDS             complement(20264..22444)
                     /gene="etk"
                     /locus_tag="KLH46_002521"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HBE0379165.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGTILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(22464..22910)
                     /gene="etp"
                     /locus_tag="KLH46_002522"
                     /gene_synonym="yccY"
     CDS             complement(22464..22910)
                     /gene="etp"
                     /locus_tag="KLH46_002522"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBE0379166.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22898..24037)
                     /locus_tag="KLH46_002523"
     CDS             complement(22898..24037)
                     /locus_tag="KLH46_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBE0379167.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24083..25243)
                     /locus_tag="KLH46_002524"
                     /pseudo
     CDS             complement(24083..25243)
                     /locus_tag="KLH46_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
     gene            complement(25322..26017)
                     /locus_tag="KLH46_002525"
     CDS             complement(25322..26017)
                     /locus_tag="KLH46_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0379168.1"
                     /translation="MLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHVATNPVTATRT
                     AKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCRMKWSDINDNH
                     LHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKHHDPLSPKTVS
                     KYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHKSDSMAARYRD
                     SRGREWDKIEIDK"
BASE COUNT         6217 a         6648 c         6906 g         6305 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgatgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccaggaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaaa
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgctact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgatgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgccg cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaacg aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgtgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gcctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga gtaagcgaca gcgatcagcg
    22321 tgaataacgc ggtcacgctg ataataaact tacggtgatc ccataactcg ccgaccagac
    22381 gaagcagatc gatctcattt tcctgagtgc tgcctggtgg cgtattcata tttttagttg
    22441 tcatcgtatt ttataaactc aaattaccgg ctgaggcgct tcgcccattc ctgactggcg
    22501 cgctccaaca taccgtagac atgttcaaat gcgtcctgac ttttacgata gggatccggg
    22561 atctctttct gttccagcca ttgcccaaac agcattgtct tcccgcggac ctcgggagca
    22621 attgcggtaa cctgagcaat atgttccgac tccattgcca gaatcagatc gtaatttcgt
    22681 gccatctcag cagtgagctt acgtccggca tgtccttcaa gagatacgcc atgattagcg
    22741 gcaacatctg ccgctgtcgc atctgcaggg tgttttacca gaccatgaac acctgccgat
    22801 ttgactttta cgcccggcag acgtttacgc agcaagcgct cgccaatggg cgaacggcaa
    22861 atgttgccgg tacaaaccac taggattgag ttaaatttta gttgggccat gtttgaatcc
    22921 accgtgaagt ctctgtcagg tcatgcaccc cggaaatcgt cggtaccagc agagaaatca
    22981 cacggttcca acgagccagc ggcgcggtag tgacataaac aatgtcgtaa ggttcaagct
    23041 ggaattccgt tccgagtatc attgccgatg cgtccttcgc attcagctga taaatattgg
    23101 cgattttgcc attctgcttg ttttgcgttg cacgaataac aaaaatcccc gtcgcatcag
    23161 cgacatcctg attcataccc tcagcgttac ccagcgcttc tgccagggtc ataccgctgc
    23221 ggtccatttt cagcgtgctc tgtttaccca cttcacccat cacgaagact ttcagatcat
    23281 cgttgcgcgg aataaataaa atatcgcccg ggtgcagcag tttgttctgg cgcaagtcgc
    23341 cacgctgcat cagcgcatag aggttcactt tggtctttac gccattctgg gtgagcacca
    23401 cgttgcgcca gtctgcatct gcggtcagac cgcccgcggc gttaatcgca tccatgatgg
    23461 tcagaggaat attggtaatc ggctgctgac ccgatttgct gacttcgccg gtgacgtacg
    23521 ctttttgcga tcggaacgcc gcgacgctga catcgacttg cgggctttca atgactgaat
    23581 cgagacgcgc tgtaatctcg ttgcgtacct gggtaagtgt tttcccggcc actttcaggc
    23641 gaccaatata ggggtagaaa atcgcaccat cggcgttaac ccagttgccg gtatcactgg
    23701 cgctacggta ttgccctgcg ggggtggtta gttccgggtg atcccagacc gtaaccatca
    23761 acacatcacc aatcccgata cgatattcat agtttgcgat ttgctgctcc agctccgggt
    23821 tagcctgcga cataatcggt ttggcgcgca gctgatcaat caatcctggc gtgacgggat
    23881 agatattgac cattttgtcc aggtcatact gattgtcagg tagttcaatg acatccttgt
    23941 tactggtcga taaattctga ccaggaacaa gggtacatgc tgtcaaagac agaacagcca
    24001 atgtcagtac cgaaaactta aaaatgttct ttttcattgg tttatattta tcagtcaatg
    24061 taatgttatg aattacgtca ggttattgtc caaccggtac ttctcgctct gccgtcattg
    24121 gatcgagctg gtatttgcgt gacagcattt gtccaccatc acgcgtcaat ggtgtccacg
    24181 aaaccaccgc gcggttgcgg ttaggtccaa tggtcatcaa gtcgaacgga atcgagatat
    24241 aaaaaccttt actaaagccg ccttcgccgt agtcatcttt agatacgtta ctgattgccg
    24301 cccatacccc taccgccacg ccgctgtcaa agcgtttggc gacgtcgatc gttgcgcctt
    24361 tatcttttgc cagatattgc ccaacgctaa gtttcatcag tacgccattg agcgtcggcg
    24421 ggttccagta agccgtcacg aaacctgtgg gcgtggaata atcggtgaag cgcatcatgt
    24481 tgtcccagtc acgctgcttc acgtagttaa cgtccacacc cagcgcccag ctggcatcta
    24541 gcgggcgata aagaagctcg gaaccgacac cggcatacat cgtttccaga taaccgccat
    24601 acacctggcc atagaaacca ttgcctaagt cggcaaagta attcgcttgc aagttgttga
    24661 gataaacgtc attgcgaacg taatcacgga tatgcgtgcg cacgcggggc agggtagagt
    24721 ccgcgggtaa cagtgaagac ttaaacttgt cgtagttgtt gtaaatattg gtgaaaatac
    24781 cgtcatcaag cagcaggtgg tcggtaaacc agtagcgggc gctggacatc agccccagct
    24841 ggaacatata gaaatcttcc gggccgccca gcgactgtga aagtgttggg ttgaaagagt
    24901 agctaaagcg atcttcacga atacggtagc cccgaccaaa ggcagaaaga tcttctgctt
    24961 caacacgttg ttgttgcagt tgctctgatt taccaggcgc tgtgccggcc agctgtttgc
    25021 gcaggctggc tacgtcggtt tcggtagtca ccatcgccat atgctcgcgc ttttgcgtca
    25081 cgctaatctt ctcaacgcct tgcggcaggt tattcaccag aatccggttg gcacgatcga
    25141 ccgcttcgcg agagtcacgg tatttgtatt gctgaccaga catatacagc gtcttatcgc
    25201 gtagctgaat ttctggcgcg tcaaagcccg cgttatactt caggtgaata agttgtatat
    25261 ttaaaatctc tttgactatc aatgaattag tataagaagg tcattattag tcaaaataaa
    25321 atcatttgtc gatttcaatt ttgtcccatt cccgtccacg gctgtcccta taccgcgccg
    25381 ccattgaatc tgatttatgt ccgagaagac gttgagcaaa cttatcgcca atctgattac
    25441 ggtatagcct cgccgacagg ctacgcagtt catggaatgt tggcgggtct ccatcaaatg
    25501 agagtcctga tgcatttctc gcctttgtaa aatacttcga tactgttttc ggggaaagcg
    25561 gatcgtgatg ctttgatgcg attatagttt cactgctgct ggcctccctg catttctgta
    25621 gtgtatcagc caatgagata ttgagcgcgt caatcgttag cgtcagcgga atggcgagct
    25681 tagcccctgt tttgctctgt tcaatgtgaa gatggttgtc gtttatgtct gaccatttca
    25741 ttctgcacaa atcgccgact ctctgccctg taacgacagc caaatccatt gctagcctca
    25801 gccagattgg gagaggttcg gctgcatggt aaatcgcgac atactcatta gctgtcagcc
    25861 ttgagcgcct tacttctgac tttgctgtgc gggttgctgt taccggattc gttgctacat
    25921 gcccctcggc tattgcctca cgaaaaacgt caacaagggt tgacctgatt aattttgcgg
    25981 aagccgcttt accttctgct acgtaggtgt ttagcattgc tgccacctct ttcgttgata
    26041 tgtcagcgag cggtttgtcc ggcaattttc ttcgga
//
LOCUS       DAEEGA010000057        25287 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEEGA010000057 DAEEGA010000000
VERSION     DAEEGA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25287
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.057"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            117..641
                     /locus_tag="KLH46_002526"
     CDS             117..641
                     /locus_tag="KLH46_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE0379169.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            722..1396
                     /locus_tag="KLH46_002527"
     CDS             722..1396
                     /locus_tag="KLH46_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBE0379170.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1412..3892
                     /locus_tag="KLH46_002528"
                     /pseudo
     CDS             1412..3892
                     /locus_tag="KLH46_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            3908..4941
                     /locus_tag="KLH46_002529"
                     /pseudo
     CDS             3908..4941
                     /locus_tag="KLH46_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5023..5361)
                     /gene="rcnB"
                     /locus_tag="KLH46_002530"
     CDS             complement(5023..5361)
                     /gene="rcnB"
                     /locus_tag="KLH46_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBE0379171.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5580..6428)
                     /gene="rcnA"
                     /locus_tag="KLH46_002531"
                     /pseudo
     CDS             complement(5580..6428)
                     /gene="rcnA"
                     /locus_tag="KLH46_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6550..6822
                     /gene="rcnR"
                     /locus_tag="KLH46_002532"
     CDS             6550..6822
                     /gene="rcnR"
                     /locus_tag="KLH46_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBE0379172.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6914..7010
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7045..7833
                     /gene="thiM"
                     /locus_tag="KLH46_002533"
     CDS             7045..7833
                     /gene="thiM"
                     /locus_tag="KLH46_002533"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBE0379173.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7830..8630
                     /gene="thiD"
                     /locus_tag="KLH46_002534"
     CDS             7830..8630
                     /gene="thiD"
                     /locus_tag="KLH46_002534"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBE0379174.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8695..9516
                     /locus_tag="KLH46_002535"
     CDS             8695..9516
                     /locus_tag="KLH46_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBE0379175.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVDERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9564..10310
                     /locus_tag="KLH46_002536"
     CDS             9564..10310
                     /locus_tag="KLH46_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE0379176.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLCGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10284..11249)
                     /locus_tag="KLH46_002537"
     CDS             complement(10284..11249)
                     /locus_tag="KLH46_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBE0379177.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11246..12249)
                     /locus_tag="KLH46_002538"
                     /pseudo
     CDS             complement(11246..12249)
                     /locus_tag="KLH46_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(12246..13496)
                     /locus_tag="KLH46_002539"
     CDS             complement(12246..13496)
                     /locus_tag="KLH46_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0379178.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NTALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCSFLFAMPLAF
                     YYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLLLGLVTAAIC
                     YGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMRTAAQGLITL
                     CCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAIIAVLFMIFFR
                     ESDNEITAIKVDDRDIALTQGEVK"
     gene            13753..14805
                     /gene="fbaB"
                     /locus_tag="KLH46_002540"
     CDS             13753..14805
                     /gene="fbaB"
                     /locus_tag="KLH46_002540"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBE0379179.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            15113..15967
                     /gene="gatY"
                     /locus_tag="KLH46_002541"
     CDS             15113..15967
                     /gene="gatY"
                     /locus_tag="KLH46_002541"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBE0379180.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            15996..17258
                     /gene="gatZ"
                     /locus_tag="KLH46_002542"
     CDS             15996..17258
                     /gene="gatZ"
                     /locus_tag="KLH46_002542"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBE0379181.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            17268..17720
                     /gene="gatA"
                     /locus_tag="KLH46_002543"
     CDS             17268..17720
                     /gene="gatA"
                     /locus_tag="KLH46_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBE0379182.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            17751..18035
                     /gene="gatB"
                     /locus_tag="KLH46_002544"
     CDS             17751..18035
                     /gene="gatB"
                     /locus_tag="KLH46_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBE0379183.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            18039..19394
                     /locus_tag="KLH46_002545"
     CDS             18039..19394
                     /locus_tag="KLH46_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBE0379184.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            19442..20482
                     /gene="gatD"
                     /locus_tag="KLH46_002546"
     CDS             19442..20482
                     /gene="gatD"
                     /locus_tag="KLH46_002546"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBE0379185.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            20588..21361
                     /locus_tag="KLH46_002547"
     CDS             20588..21361
                     /locus_tag="KLH46_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE0379186.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(21443..22342)
                     /gene="yegS"
                     /locus_tag="KLH46_002548"
     CDS             complement(21443..22342)
                     /gene="yegS"
                     /locus_tag="KLH46_002548"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBE0379187.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            22748..23065
                     /locus_tag="KLH46_002549"
     CDS             22748..23065
                     /locus_tag="KLH46_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379188.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(23407..24768)
                     /gene="yegQ"
                     /locus_tag="KLH46_002550"
     CDS             complement(23407..24768)
                     /gene="yegQ"
                     /locus_tag="KLH46_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBE0379189.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(24915..25246)
                     /locus_tag="KLH46_002551"
                     /pseudo
     CDS             complement(24915..25246)
                     /locus_tag="KLH46_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
BASE COUNT         6630 a         5814 c         6395 g         6448 t
ORIGIN      
        1 tttaaatcaa taaaacaata tgtttacatt gcggcagctt ttttgatgtg agaaatttgt
       61 ttccaagaga tacagactct taacaaccaa aaataaattt tattaaggaa catattatga
      121 aacgttcaat tattgctgcc gctgtctttt cttctttttt tatgagcgct ggagtatttg
      181 ctgcagacgt tgataccgga acattaacca ttaaaggaaa tattgcagaa tctccgtgta
      241 aattcgaagc gggtggtgat tcagtaagta ttaatatgcc gactgtacca accactgtct
      301 ttgaaggtaa agctaaatat tctacctatg atgatgcagt cggtgtaacc agcagcatgt
      361 taaaaattag ctgcccgaaa gaagttgctg gtgtaaaact ctcgttgatc acaaacgaca
      421 aaataaccgg taacgataag gcgatagcca gtagcaacga taccgtaggt gataacagcg
      481 atgtcctaga tgtttctgca ccttttaaca ttgagagtta taaaacagcg gaaggtcaat
      541 atgctattcc gtttaaagca aaatacctga aactgacaga taactcagtg caatcaggtg
      601 atgtgttatc ttctctggtt atgcgtgtgg cgcaggatta ataaataaaa ttgcaagttt
      661 ctccaggtaa taaacatggc tgctatcccc atggcggctt tttaatttaa gaggcattaa
      721 gatgaaagga ttattatctt tactcatttt ttctatggtc cttcctgcac atgccggaat
      781 tgttatctac gggacgcgca ttatttaccc ggcagaaaat aaagaagtta tggtgcagtt
      841 gatgaaccag ggaaatcgtt cttcgctgct gcaggcgtgg attgatgatg gcgatacgtc
      901 gttaccacca gaaaaaattc aggttccttt catgttaacg ccaccagtgg caaaaatagg
      961 ggcaaattcc gggcagcaag taaaaatcaa aattatgccg aataaactgc ccactaataa
     1021 agaaagcatt ttttatctga atgttctgta tattccacca aatagtccag agcaagaagg
     1081 caagaatgca ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc ggccagcggg
     1141 tattgctccg gtaaataaag cgacctttaa aaagttactg gtaaatcgca gtggcaatgg
     1201 attggtgata aaaaatgact cagctaattg ggtgacgatt tcggatgtca aagctaataa
     1261 tgttaaagtc aattatgaaa ctattatgat tgccccctta gaaagtcaga gtgttaatat
     1321 caaaagtaat aatgcaaata actggtatct gaccattatc aatgaccacg gcaactatat
     1381 tagtgacaaa atttaattgc aggagctgcc tatgttgaga atgaccccac ttgcatcagc
     1441 aatcgtagcg ttattgctcg gcattgaagc ttatgcagct gaagaaacct ttgataccca
     1501 ttttatgata ggtggaatga aagaccagca ggttgcaaat attcgtcttg atgataatca
     1561 acccttaccg gggcagtatg acatcgatat ttatgtcaat aagcaatggc gcgggaaata
     1621 tgagattatt gttaaagaca acccgcaaga aacatgttta tcaagagaag ttatcaagcg
     1681 gttaggcatt aatagcgata acttcgccag cggtaagcaa tgtttaacat ttgagcaact
     1741 tgttcagggt gggagctata gctgggatat cggggttttt cgtctcgatt tcagtgtccc
     1801 gcaggcttgg gtggaagaac tggaaagtgg ctatgttcca ccggaaaact gggagcgggg
     1861 tattaatgcg ttttatactt cttattatgt gagtcagtat tacagcgact ataaagcgtc
     1921 gggtaacaac aagagtacat atgtacgttt taacagcggg ttaaatttac tggagtggca
     1981 actgcattct gatgccagtt tcagtaaaac aaataacaat ccaggggtgt ggaaaagcaa
     2041 taccctgtat ctggaacgtg gatttgccca atttctcggc acgcttcgcg tgggtgatat
     2101 gtacacatca agcgatattt ttgattctgt tcgcttcagc ggtgtgcggt tgtttcgtga
     2161 tatgcagatg ttgcctaact cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca
     2221 gagtaacgcg ctggtaacta ttgaacagaa tggttttgtg gtttatcaga aagaggttcc
     2281 tcctggcccg tttgcgatta cagatttgca gttggccggt ggtggagcag atcttgatgt
     2341 tagcgtgaaa gaggcggacg gctcggtaac cacctatctg gtgccttatg cagcggtgcc
     2401 aaatatgctg caacccggcg tgtcgaaata tgattttgcg gcgggccgta gccatattga
     2461 aggggcgagc aagcaaagtg attttgtcca ggcaggttat cagtatggtt ttaataactt
     2521 attgacgctg tatggcggca cgatggttgc taataattac tatgcgttta ccctctgaac
     2581 gggttggaac acgcgcattg gcgcaatttc cgtcgatgcc acgaagtcgc atagtaaaca
     2641 agacaacggc gatgtgtttg acgggcaaag ttatcaaatt gcctacaaca aatttgtgag
     2701 ccaaacgtcg acgcgttttg gtctggcggc ctggcgttat tcgtcgcgtg attaccggac
     2761 atttaacgat cacgtttggg caaacaataa agataattat cgccgtgatg aaaacgatat
     2821 ctatgacatt gccgattatt accagaacga ttttggccgt aaaaatagct tttctgccaa
     2881 tatgagccag tcattgccag aaggctgggg ttctgtgtcc ttaagtacgt tatggcgaga
     2941 ttactggggg cgtagcggca gcagtaagga ttatcagttg agttattcca ataactggcg
     3001 gcggataagc tataccctcg cggcaagcca ggcgtatggc gagaatcatc atgaagagaa
     3061 acgttttaat atttttatat caattccctg tgattggggt gatgacgtta cgacgcctcg
     3121 tcggcaaata tatatgtcta actcaacgac gtttgatgat cagggttttg cctcaaataa
     3181 tacgggatta tcaggaacag tagggagtcg ggatcagttc aattatggtg tcaacctgag
     3241 tcatcaacat cagggaaatg aaacgacagc tggggcgaat ttgacctgga acgcgccggt
     3301 tgcgacagtg aatggcagtt atagtcagtc gagtacttat cgacagactg gagccagtgt
     3361 ttcagggggc attgtcgcct ggtcgggtgg cgttaatctg gcgaaccgtc tttccgaaac
     3421 gtttgctgtg atgaatgcgc caggaattaa agatgcttat gtcaatgggc aaaaatatcg
     3481 cacaacaaac cgtaatggag tggtggtata cgacggaatg acaccttatc gggaaaatca
     3541 cctgatgctg gatgtgtcgc aaagcgatag cgaagcagaa ttacgtggca accggaaaat
     3601 tgccgcccct tatcgcggcg cggttgtact ggttaatttt gataccgatc agcgcaagcc
     3661 ctggtttata aaagcgttaa gagcggatgg tcaaccatta acgtttggtt atgaagtcaa
     3721 tgatatccat ggtcataata ttggcgttgt cggccaggga agccagttat ttattcgcac
     3781 caatgaaata ccgccatcgg ttaatgtagc aattgataag caacaaggac tttcatgcac
     3841 aatcaccttc ggtaaagaga ttgatgaaag tagaaattat atttgccagt aatagtcagg
     3901 tggttctatg gaaattcgca taatgctatt tatattaatg atgatggtta tgcctgtgag
     3961 ctatgcggca tgttatagtg agttatctgt tcagcacaat ttggttgttc aggggaattt
     4021 tgcacttact caaacacaaa tagcgacata tgagcataat tttaatgatt cgtcatgcgt
     4081 gagtacaaat actatcaccc ctatgagccc atcggatatt attgttggac tttataacga
     4141 taccattaaa ttaaatttac attttgaatg gaccaataaa aacaacatca cgttgtcaaa
     4201 taatcagagc agtttcacca gtggttattc ggtaacggtg acacctgcgg ccagtaatgc
     4261 aaaagtgaat atttctgcgg ggggcggtgg ttcagtgatg attaatggtg ttgcgacatt
     4321 atccagtgct tcatcatcga cacgcgggag tgccgcagta caatttctac tgtgtttatt
     4381 aggtggcaag tcatggggtg catgtgtaaa tagctacaga aatgcattgg cacaaaatgc
     4441 aggtgtctat tccttcaatc tgacattgtc atacaacccg ataaccacaa cctgcaaaca
     4501 ggacgattta ttaattactt tagacagtat tcccgtttca caattaccag ccacaggtaa
     4561 caaagcaaca ataaatagta aaaaaaggga tattattctg cgttgtaaaa atttattagg
     4621 tcaacaaaat caaacatcac ggaaaatgca ggtgtattta tcaagttctg acttgttaac
     4681 caacagcaac acaatactga aaggtgcgga agataatgcg taggatttat tcttgaaagt
     4741 aatggttcgc cagtcacact tttaaatatc actaacagca gtaaaggata cacaaattta
     4801 aaagaaattg cggcaaagtc aaaacttaca gatacaacgg tttcaattcc gataacagcc
     4861 agttactacg tctacgatac aaacaaagtt aaatctggcg cactggaggc aaccgcatta
     4921 atcaacgtga aatacgacta ataacaagca aaacgagcaa gtggctaata ataaaaaata
     4981 acaaggtgtc tgcatttccc cctcatgatg agggggcttt ttttagcgat gataaaaaat
     5041 ctcaccgtcg taggctttaa tgattttacc gtcggtgtcg ctgatcaaca cgtacgcgcc
     5101 acccatatag gtccagtgcg tcccggcatc aggcgcgggc aggttacgta actgccactg
     5161 cttaatgttg tactcatcgg tgcggtacat ctctgggacg gtatcacctg gtttgaaatg
     5221 cttaaagtca gcaatgaaac tacttaactc atacttagaa attcccgagg tattggtcga
     5281 acccgcggtg gctggtgcgg cccaggcggc actggtaacc agcaaaagca cacccagcaa
     5341 catcttattt ttaatggtca taaactctcc actttgggga tctgtaatat cagtcgcata
     5401 tctttcctgt catacaatat aactgacaag cgatattttg ttagtgatcc catgatttcg
     5461 taaaaagtgt aaatgaaagg atgccctaaa agcatcctta gcgagcagag agtcagcgga
     5521 aatagctgat ttaagaatca tcctgggctg aagataacat gcaagcctaa aggattgagt
     5581 tatcgcatta tgcccatgaa gccatgtacg cccatataca caccgactaa gccaatcaac
     5641 agactggaaa aatagggggc gcgtttagcg agagtgttaa atccgctcca gcgttttgcg
     5701 acctgctgaa cgctgattgc tgcgccaacg cctacggtga caagcgttaa cgccaggcca
     5761 atgctgaaac tgacgaccag tgttgcgccc agtgtcaggg ctttcaactg aatgcatatc
     5821 aacagcacgg taattgctgc cgggcagggg ataaagccac cggttaagcc aaataacaaa
     5881 atttgccagt tgatgacctc tctaccatca aagcgtcgtt taatgtcatt ggcatgggct
     5941 cgtgcatggg catcctgata ctcgccatat tcgtgatggt gatgatgtcc ttggtcgtgg
     6001 tgatgttcgt ggtcttgatg atgttcgtga tcgtgatgat ggtgatgatg ctcataatca
     6061 tgcccgtgca tattctccag ccagttgcgt tcgccgcgct aggtacgcca gaacatccag
     6121 aacgcggtgc caataatgat cactgcggaa atcagctgga gccacggttc tgctgattga
     6181 gcagtaaagc gcttactgat caccatcccg ccaaaggcaa tcaaccagac caccgcggta
     6241 tgcgaaatgg ttgctgccag tccgagcatc accgcctgtt taatggtgcc tttgatggcg
     6301 atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg aagcgcacca
     6361 agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag agttgtaaat
     6421 tcggtcatga taataattct tagtattaat tcggcaatct gattctactc ccccccagta
     6481 cctgatacta cccccccagt agattaatag tgctatgatt tttcatattc ttgttaacca
     6541 ggtgttgcca tgtctcatac aatccgtgat aaacagaaac tgaaagcgcg tgccagtaag
     6601 attcagggcc aggtcgtggc gctcaagaaa atgctcgacg agccgcacga atgcgctgca
     6661 gttttacaac agattgctgc tatccgtgga gcggtaaacg gtctgatgcg ggaagtgatt
     6721 aaaggtcatc tgacggaaca catcgttcac cagggggatg agctaaaacg tgaagaagat
     6781 ctggatgtcg ttctgaaggt gctggattca tatatcaaat aatttattaa cgcgattgta
     6841 aaactgccgt ttttcctcgt ttacaacggg ggcgctggac attaccatcc tcctctgcga
     6901 tttatcatcg caaccaaacg actcggggtg cccttctgcg tgaaggctga gaaatacccg
     6961 tatcacctga tctggataat gccagcgtag ggaagtcacg gaccaccagg tcattgcttc
     7021 ttcacgttat ggcaggagca aactatgcaa gtcgacctgc tgagttcagc gcaatctgcg
     7081 cacgcgttac acctttttca ccaacattcc cctcttgtgc actgcatgac caatgatgtg
     7141 gtgcaaacct ttaccgccaa taccttgctg gcgctcggtg catcgccagc gatggttatc
     7201 gaaaccgaag aggccagtca gtttgcggct atcgccagtg ccttgttgat taacgttggc
     7261 acactgacgc agccacgcgc acaggcgatg cgtgctgccg ttgagcaagc aaaaagctct
     7321 caaacacgct ggacgcttga tccagtagcg gttggtgcgc tcgattatcg ccgccatttt
     7381 tgtcatgaac ttttatcttt taaaccggca gcgatacgtg gtaatgcttc ggaaatcatg
     7441 gcattagctg gcattgctaa tggcggacgg ggagtggata ccactgacgc cgcagctaac
     7501 gcgatacccg ctgcacaaac actggcacgg gaaactggcg caatcgtcgt ggtcactggc
     7561 gaggtggatt atgttaccga tggacatcgt atcattggta ttcacggtgg cgatccgtta
     7621 atgaccaaag tggtaggaac tggctgtgca ttatcggcgg ttgtcgctgc ctgctgtgcg
     7681 ttaccaggcg atacgctgga aaatgtcgca tctgcctgtc actggatgaa acaagccgga
     7741 gaacgcgcag tcgccagaag cgaggggcca ggcagttttg ttccacattt ccttgatgcg
     7801 ctctggcaat tgacgcagga ggtgcaggca tgaaacgaat taacgctctg acgattgccg
     7861 gtactgatcc gagtggtggt gcggggattc aggccgatct taaaaccttc tcggcacttg
     7921 gcgcttatgg ttgctcagtt attactgcac tggtggcgca aaatacccgt ggcgtacagt
     7981 cggtgtatcg cattgagcct gattttgtcg ccgcccagct cgattcggtg ttcagcgatg
     8041 tgcgaatcga taccactaaa atcggtatgt tggcggaaac cgatattgtt gaagcggtgg
     8101 cagaacggtt gcaacgttat cagatccaaa acgtggtact cgacaccgtt atgctggcaa
     8161 aaagcggcga cccgctgctt tcaccttcgg cggttgctac gctgcgcagt cgattattgc
     8221 cacaggtttc attaataacg ccaaacttgc ccgaagctgc cgccttgctc gacgcgccac
     8281 acgcgcgcac cgaacaggaa atgctggaac aagggcgatc gctgttggcg atgggctgtg
     8341 gcgcagtgct aatgaaaggt ggtcatctgg atgatgagca aagcccggac tggctgttta
     8401 cccgcgaggg tgaacaacgg tttaccgcac cgcgcattat gaccaaaaac acccacggca
     8461 ctggttgtac actctctgcg gcgttggctg cactacgccc gcgccataca aactgggctg
     8521 acaccgtaca ggaggcaaaa atctggcttt catcggcgtt agcccaggcc gacacgctgg
     8581 aagttggtca cggtattggt ccggttcacc acttccacgc ctggtggtga gcaccgtact
     8641 atagctgctt tggttattgt cagcctttgg cactgacatt cagggatgaa accaatgcag
     8701 ctaagaatta ccagtcgaaa aaaactcacc gctctattat gcgctcttgg gctaatttcc
     8761 atcgttgcaa tttatccgcg tcagacggtg aattttttct attcgacagc agttcagatt
     8821 acagactaca tccacttcta cggttatcgc ccggttaaat ctttcgctat tcgcattcct
     8881 gccagttaca ccattcacgg attagatgtt tcgcgctggc aggagcgtat cgactggcag
     8941 cgggtggcaa aaatgcgcga caacgatatc cgcttacagt ttgcttttat taaggctacg
     9001 gaaggcgaaa agctggtgga cccctatttt tcgcgtaact ggcaattaag ccgcgaaaat
     9061 ggcctgctgc gcggggcgta tcattatttc tccccgtcgg tatccgcttc agttcaggcg
     9121 cgattatttc tgcaaacggt ggatttctca caaggcgatt tccctgccgt gctggacgta
     9181 gacgaacggg gaaaattatc ggcaaaagaa ttacgcaagc gggtaagtca gtggctaaaa
     9241 atggtcgaaa aacgcacggg aaaaaagccg attatttact caggagccgt tttttatcac
     9301 accaatctgg cgggctattt caatgaatat ccgtggtggg tggctcacta ttatcaacgt
     9361 cgtccggaca atgacggcat ggcctggcgc ttctggcagc attccgaccg tggacaggta
     9421 gatggcatca atggtccggt ggattttaat gtgtttaatg gcacggggat gagttgcagg
     9481 cattcgttga tgggattaaa gaaacgcctt aaataagaag cagtgatata ctcccaccag
     9541 actccctggc aggaaaaaga caaatggaac aagcgcatac ccagctgatt gcccaactga
     9601 acgagcgtat tttagcggcg gataacacgc cgctttatat taagtttgcc gaaacggtaa
     9661 aaaatgccgt gcgcagcggg gtgctggagc atggcaatat tttgcccggt gagcgtgatt
     9721 taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag gcgctggaag
     9781 aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac atcttcgaat
     9841 actcgttgaa agaagcgaga ggtttttctc agcaggtggt attgtgcggg aaaaagcccg
     9901 atacgctatg ggttaataag cgtgtcgtga aatgtcccga agaagttgcg cagcagctgg
     9961 cggtcgaagc gggaagtgat gtctttttgc ttaagcgtat tcgctatgtc gatgaagaag
    10021 cggtatcgat tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt gatgccatcg
    10081 gaatttctct ttatgactat ttccgcagcc agcacattta cccacaacgt acgcgttccc
    10141 gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca tattcagtta gatagcaaaa
    10201 taccggtgct ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct attgagtaca
    10261 gcattagcca ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag ttcctcgcgc
    10321 gttggcgcac aatcaccgcc ccgatgcccg acaacccacg acgccactgc attgcccagt
    10381 aatacggcat ccgccagtgg cagaccagag gccagcccgg caagtacgcc acctgcatga
    10441 ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa atgccggaat gcagccagaa
    10501 gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac gaacgatcaa cggcgcggca
    10561 aatttctcct gccattgctt gccaagtgtt gttatctctg cggataaagc aaaacgttcg
    10621 gcggcaatct cagcctcttg acgattgagt gacactaaag gtcgacaggc catgatccgc
    10681 gccagtaatg cgtccgggat atcgccaata cgtgggcaaa aatcgataaa cggcgtcacg
    10741 tcttgcagct tttccagcca ttccactaac aattcgccgc agggcgaggc cagttgataa
    10801 ccggaaaaat agagcaggtt gccaggcgca acggttaatc gtgccaacca ttggcgattc
    10861 cattgatttt ccacaccgct gaatgacata aaagtgcgtt cgccatccgg ctcaaccagt
    10921 gccagacacc agccgttatc gctttcggcg ttatcgatca ggctgattaa cccctctttc
    10981 gccatccggt tgcgaataat ctccgcccac acgccctgac cgagcggcaa ggcattaccc
    11041 gcttcgatgc cgaggcgctt taacgccacg gcaatattca gtgcgcagcc gccaacgtta
    11101 acgctctgct gtttcagttc gatatcgcac ccacgccagg ggagggcata agcgtcggcg
    11161 atcacgtcaa tgaccgccgc gccgacgacc atcaccggct gacgcgtggt taattcaggc
    11221 agcagtgtgt gtaatcgagc gccgctcata ccgcctcccg ttgttgacga tatttcgcca
    11281 gcgctgtggc atagcggttg aaatcaagct gatttaccgc atccagctcc gcctttaacg
    11341 caggatcgac agcgttaatg ccatgcaacg cgccgcaaat cgccgtcgcc atagcaccta
    11401 tggtgtctgt gtcgccgcca agattggcgc acaggacggc gcagcggttt gggtcggttt
    11461 gtgccagttc aactagcgca atggcgcacg gaacggactc aatagtgctg gtgcctgcgc
    11521 caacgacctg gtaaagctgt tcgctggcgg attcggtgcc gtcggcatta cgcacaattt
    11581 tcagcgcaat ctccagacgt gccgccagtg aggcgctgaa cgtggtggtg cgtttttgct
    11641 gtgcatgtcg cgcaattgaa ggcaatgaat caacaatcgc tgaccagcgt tctccgtcaa
    11701 tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc aaccgccaga tcggatttat
    11761 gggtcgggct ggacgccagc gccacatcat caataaagga atcaacatca cgcgctggaa
    11821 gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt ggttacgccg ttgttttcca
    11881 gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc aatcttcgag gtcggaccta
    11941 gtacgttttt gttaaaggcg tcgaaacgca acgcccagtc gagaatatta cgcccaatca
    12001 gattcggatc gatcttgcct tcacattcca gtaacgcatc cgccagacac aggccatcga
    12061 ggtatcgtcg gtgaattcgg cgcggttaaa ataacaggcc gcgttattct cctttggtcc
    12121 aggcagaaaa cggtcaatcc agccaaagtg tgctttaacg cggctgcgtg gccaaagctc
    12181 ggagggcatc cccatcgcat cccctaacgc ctgcccataa agagcaccga gaatacgttc
    12241 tgttttcatt taacttcccc ttgtgtcaac gcaatatctc gatcatcgac cttgatagcc
    12301 gtaatttcgt tgtcggattc gcggaaaaaa atcatgaaca gcacggcgat aatggcaatc
    12361 atcaccgcgc cgaaagtcca catcccggac cagttgaaag tcagtccgtt taccggttcc
    12421 tgataagcga acatcttttc catcatcaca ccgccaagac gatagccgag caaactgccg
    12481 aagccctggc agcagagcgt gatcagcccc tgcgcagcgg tacgcatatg cacgggggct
    12541 tttttatcga catagatgta agcggtaacg tagtaaaaat cgtaacttac gccgtgaagc
    12601 aaaataccga ggaacagtaa cgcgtaggtg aaatattcat ccgcactacc gtaaataaag
    12661 aagccatagc agatcgcagc ggtgaccaga ccaagcaata ataccttttt gataccaaag
    12721 cgtttagtga aaaacggcaa tgccagcata aagaagattt cagagaactg gccgagcgtc
    12781 atccagccgg tggcgttttt catgccaact tcggtcagat aaccgttggc aaagatgtaa
    12841 tagaacgcca gtggcatcgc aaacaggaat gaacagaaga aaaagacgag gaagttttta
    12901 tcgcgcagca ggatcagcgc atccaggccg agcatgactt taatgtccat tttgccggtg
    12961 ctttttggcg gcgtgtcggg caggaaaaac gcaaacacac cgagcagagc agtgttagtc
    13021 ggtgagatat cggcataccc cagcatttgc ggcaagaaac cacatgccag accggaggca
    13081 atccagccga tagtgcccat cacacgaatg cgtgggaaat cacgctccac atccggcacg
    13141 ttggcaaaag cgatgctgtt agtcagcgca atggtcggca tataggttag cgagtaggcc
    13201 agcagtaacg gaaagaaccc ggcaaaagtg gtctgttgcg cagcgaaata catcagcaca
    13261 gcaccagcga acatcaatac cgccagcact ttctgcgccg agaaaaagcg gtcagtgatg
    13321 gagccaacca gaattggcga caggatcgcc gcaatggctg tgcaggcata cgaccagcca
    13381 atttctccgg cactaaaacc gcttttactt aaccacaacc acaatggcac aaaccacgcg
    13441 ccccagataa accattcaac aaacatcatg aacgacagct ttgctgttgt tttcatcttt
    13501 taattccttc atgacaggta atgtaaggta cgcgatgaca ataccacata ataatgcctt
    13561 ttgaatacct ttgcggtgaa aacttgaccg cgctaacatt ttttctgatg aatcgagcca
    13621 acagaaaacg ctgaaaaaac atccaaaaga tggaaaaact cgcccgtaac gactaggatt
    13681 atgattgccc gcaaaaggcg ggccaggaca atccatagcc gatatccaat cggaatttac
    13741 gggagcatag taatgacaga tattgcgcag ttgcttggca aagacgccga caacctttta
    13801 cagcaccgtt gtatgactat tccttctgac cagctttatc tccccggaca tgactacgta
    13861 gaccgcgtga tgattgacaa taatcgcccg ccagcggtgt tacgtaatat gcagacgttg
    13921 tacaacaccg ggcgtctggc tggcacagga tatctttcta ttctgccggt tgaccagggc
    13981 gttgagcact ctgccggagc ttcatttgct gctaacccgc tctactttga cccgaaaaac
    14041 attgttgaac tggcgatcga agcgggctgt aactgtgtgg catcaactta cggcgtgttg
    14101 gtgtcggtat cgcggcgcta tgcgcatcgc attccatttc tcgtcaaact taatcacaac
    14161 gagacgctaa gttacccgaa tacctacgat caaacgctgt atgccagcgt ggagcaggcc
    14221 ttcaacatgg gcgcggtggc ggttggtgcg actatctatt ttggctcgga agagtcacgt
    14281 cgccagattg aagaaatttc tgcggccttt gaacgcgcgc acgagctggg catggtgaca
    14341 gtgctgtggg cctatttgcg aaactccgcc tttaagaaag atggcgttga ttaccatgtt
    14401 tccgccgacc tgactggtca ggcgaaccat ctggcggcaa ccataggtgc agatatcgtc
    14461 aaacaaaaaa tggcggaaaa taacggcggc tataaagcga ttaattacgg ttataccgac
    14521 gatcgcgtgt acagcaagtt aaccagcgaa aacccgattg atctggtgcg ttatcagtta
    14581 gctaactgct atatgggccg ggctgggttg ataaactccg gcggtgctgc aggcggtgaa
    14641 actgacctca gcgatgcagt gcgtactgcg gttatcaaca aacgcgcagg cggaatgggg
    14701 ctgattcttg gacgtaaggc gttcaagaaa tcgatggctg acggcgtgaa actgattaat
    14761 gccgtgcagg acgtttatct cgatagcaaa attactattg cctgatacgc ctcatacgaa
    14821 cagcaccacc ccgtaggacg gataagacgt ttacactgca tccggcaacc tatgcctgat
    14881 gcgacgcgga agcgtcttat catgcctaca tagcactgcc acgtatgttt acaccgcatc
    14941 cggcataaaa acacgcgcac tttgctacgg cttccctatc gggaggccgt ttttttgcct
    15001 ttcactcctc gaacaatttt catattgttg tttttgtgat cattatctcg acatttaaaa
    15061 gcaaataatt tcattatatt ttgaaatcga aagcaaacga caagatatga aaatgtacgt
    15121 ggtatcgaca aagcagatgc tgaacaacgc acagcgcggc ggttatgcgg ttccggcatt
    15181 caatattcac aatctcgaaa cgatgcaagt ggtggtggaa accgctgcca acctgcatgc
    15241 gccggtcatc atcgccggaa cgcctggcac atttactcat gctggtacag aaaatctgtt
    15301 ggcgctggtc agcgcgatgg cgaagcaata tcaccatcca ctggcaattc atctcgacca
    15361 tcacacgaaa tttgacgata tcgctcagaa ggttcgttct ggcgtgcgct cagtcatgat
    15421 tgacgcctcg catttgcctt ttgcgcaaaa tatttcacgg gtcaaagagg tggtggattt
    15481 ttgccatcgc tttgatgtca gcgtcgaggc ggagctgggg caacttggcg gccaggaaga
    15541 tgatgtgcaa gtcaatgaag tcgatgcgtt gtacaccaac cccgcacagg cgcgtgaatt
    15601 tgccgaggca accgggattg attccctggc ggtcgccatc ggcacggctc acggtatgta
    15661 tgccagcgca ccggtgcttg atttttctcg actggagaac attcgccagt gggtgaactt
    15721 accgctggtg ctgcatggcg cgtcaggatt atcgactaag gatattcagc aaaccatcaa
    15781 actggggata tgcaaaatca acgttgcaac ggagctgaaa aatgccttct tgcagtcgtt
    15841 aaaaaattac ctgaccgagc accccgaagc gaccgatccc cgggattatt tgcagtcggc
    15901 taaatccgca atgcgcgatg tggtgagcaa agtgattgcc gattgtggct gcgagggcag
    15961 ggcataacgc acctgccatt taacaaggaa aaaacatgaa aacgttaatt gcccggcata
    16021 aagctggtga acatatcggc atatgttcag tctgttctgc ccatccgttg gttatcgaag
    16081 cggcgctggc atttgatcgc aacagcacgc gcaaagtgct gattgaagca acgtcaaacc
    16141 aggtcaatca atttggcggt tataccggaa tgacaccggc agactttcgc gaatttgttt
    16201 ttacgattgc cgataaagtt gggtttgcac gcgaacgcat tattctcggc ggcgatcatc
    16261 tgggaccaaa ctgctggcag caagaaaatg cggatgcggc gatggaaaaa tccgtcgagc
    16321 tggtaaaagc atatgttcgt gctggcttca gtaaaattca tcttgatgcg tcaatgtcct
    16381 gcgcggggga taccataccg ttagcaccag aaacggttgc ggaacgagct gctgtgcttt
    16441 gctttgctgc ggaaagtgtg gcgacagatt gccagcgtga gcaactgagc tatgtcattg
    16501 gcactgaagt tccggttccg ggcggtgagg ccagcgccat tcagtcagta cacatcaccc
    16561 atgttgaaga tgccgccaat actttacgta cgcatcaaaa ggcctttatt gcccgtgggc
    16621 tgacagaggc gttaacacgt gtgattgcca tcgtggtgca gccgggtgtg gaatttgatc
    16681 acagcaatat tatccattat cagccgcagg aagcgcaggc gctggcgcaa tggatagaaa
    16741 acacccgaat ggtttatgaa gcacattcta ccgattacca gacccggacg gcttattggg
    16801 aattagtccg cgatcacttt gcaatattga aagtcggtcc cgcattaacc tttgctttac
    16861 gcgaggcgat atttgcactg gcacaaattg agcaggaact tatcgccccc gaaaatcgca
    16921 gcggttgcct ggcggtaatt gaagaagtga tgctcgacga accgcaatac tggaaaaaat
    16981 attatcgcac gggttttaac gattcattac tggatattcg ttacagcctg tcggatcgta
    17041 ttcgttatta ctggccgcat agtcggatta aaaatagcgt tgaaacgatg atggtgaatc
    17101 tgcaaggcgt ggacatccca ctgggcatga ttagtcagta tcttcccaag caatttgaac
    17161 gcattcagtc cggggaatta tcagcaatgc cgcatcagct tattatgaat aaaatttatg
    17221 atgttttgcg cgcctatcgc tacggctgtg cggaataagg acggtatatg actaacctgt
    17281 ttgttcgtag cggaatttct tttgtcgatc gtagcgaagt tttaacctat atcggtaatg
    17341 agatgctcgc gaaaggtgtg gttcatgata cctggccaca ggcattaatt gccagagaag
    17401 cagaattccc taccgggata atgcttgagc agcacgccat tgcaataccg cattgtgagg
    17461 cgattcatgc taagtcgtca gccatttatc tgttaaggcc aacaaataaa gttcattttc
    17521 agcaagcgga tgatgataac gacgtggcgg tatcgttggt tattgcgttg attgtggaaa
    17581 atccgcagca gcaattgaaa cttttacgct gtttatttgg caagttacaa cagcccgata
    17641 tcgtcgagac actaattact cttcctgaaa ctcagttaaa ggaatacttc acaaagtatg
    17701 ttttagattc agacgaataa atccctctgt aacaataaaa agtactattt atgaaacgca
    17761 agattattgt cgcttgcgga ggcgcggttg cgacctctac gatggcggcg gaagaaatta
    17821 aagagttgtg tcagagtcat aatattcctg ttgaattaat ccagtgtcgg gttaatgaaa
    17881 tagaaaccta tatggatggt gtgcatttga tatgtaccac tgccagggtg gatcgtagtt
    17941 ttggcgatat tccgttagtt cacggcatgc cttttgtttc tggtgtcggt atcgaagcat
    18001 tacaaaataa aattctgact atcttacagg ggtgacctat gttttcagaa gtcatgcgtt
    18061 atattctcga cctcggccct acagtgatgc tgccggttgt catcattatt ttttctaaaa
    18121 tattaggcat gaaggcaggc gattgcttta aagcgggtct gcatatcggg attggctttg
    18181 ttggcattgg ccttgtgatt ggcttaatgc tggattccat tggtccggcg gcgaaagcga
    18241 tggcggaaaa tttcgacctg aatctgcatg tggtcgatgt cggctggccg ggctcttcac
    18301 caatgacctg ggcgtcgcaa attgcgctgg tggcgattcc gattgcgata ttggttaacg
    18361 tggcgatgtt actgacccgt atgacgcggg tggtaaatgt tgatatctgg aatatctggc
    18421 atatgacctt caccggcgcg ttgctgcacc tggcaaccgg ttcatggatg atagggatgg
    18481 cgggtgtggt aattcacgcg gcgtttgttt ataagctctg cgactggttt gcccgcgata
    18541 cccgaaattt ctttgagctg gaaggtattg ctattccgca cggtacgtcg gcgtatatgg
    18601 ggccgattgc ggtgctggtc gatgctatca tcgagaaaat cccaggcgtt aaccgaatta
    18661 aatttagcgc cgacgatatt cagcgcaaat ttggcccttt tggcgaacct gtcaccgtgg
    18721 gttttgtgat ggggctgatt atcggcatcc tcgcgggtta cgatgtcaaa ggtgtattgc
    18781 agctggcggt aaaaacggcg gcagttatgc tgctgatgcc acggatgatt aaacccatca
    18841 tggatggttt aacgcccatc gctaagcagg cccgtagtcg tttacaggcg aagttcggcg
    18901 gtcaggagtt cctgattggc ctggatcccg cattactgct ggggcatacg gcggtggtat
    18961 cggcaagcct gatttttatt ccgctcacca ttttaattgc tgtttgtgtt ccgggtaatc
    19021 aggtactgcc gtttggcgat cttgccacta tcggcttctt cgtggcgatg gcggtcgcgg
    19081 tgcatcgtgg aaatctgttc cgcaccttaa tctcgggtgt catcattatg agcatcaccc
    19141 tgtggatcgc gacgcaaact attggtttgc acacccaacc ggcggctaat gctggggcgt
    19201 taaaagccgg gggtatggtg gcttcaatgg atcagggcgg ttctcccatt acctggttac
    19261 tgattcaggt tttctccccg caaaatattc ccggtttcat tattatcggc gcaatttatc
    19321 tgaccggtat tttcatgacc tggcgtagag cgcgtggttt tactaaacaa gagaaagccg
    19381 ttctcgcaga ataattttta cctgaggggg aggttatccc ccttaacaat caggagtttt
    19441 tatgaaatca gtagtgaatg atactgatgg tatcgtgcgc gttgcaaaaa gcgtcattcc
    19501 tgaaattaaa catcaggatg aggtgcgggt aaaaattgcc agctcgggct tatgtggttc
    19561 cgatttaccc aggatattta aaaatggtgc acattattat ccaataacgt taggccatga
    19621 atttagcggc tatattgatg cggtgggatc cggtgttgat gatttacacc ctggcgatgc
    19681 ggttgcctgt gtgccgttat taccctgttt tacttgtcca gagtgtttga aagggtttta
    19741 ttcccagtgc gcaaaatatg attttattgg ctcgcggcgt gatggtggat ttgctgaata
    19801 tattgtcgtt aagcgaaaaa atgtctttgc tctacccacg gatatgccta ttgaggatgg
    19861 ggcttttatt gagccgatta ccgttggcct gcatgctttt catttagcgc aaggttgtga
    19921 gaataaaaac gttattatta ttggtgccgg aaccattggc ctgctggcta ttcagtgcgc
    19981 tgtcgcgctg ggagcaaaga gtgtgacggc gatcgacatt agctcagaaa aactggcact
    20041 ggcaaaatct ttcggtgcga tgcaaacatt taacagtagc gaaatgagcg cgccgcaaat
    20101 gcagagcgtt ttacgcgaac tgcgctttaa tcagcttatc ctcgagacgg ctggcgtacc
    20161 gcaaactgtc gaactggcgg tagagattgc cggtcctcat gcccaactgg cgctggtggg
    20221 cacgttgcat caggatctgc atttaacatc ggcaacgttt ggcaaaatat tgcgtaaaga
    20281 gctgacggtt atcggcagtt ggatgaacta ttccagccct tggccggggc aggagtggga
    20341 aacggcgagc cggttgctga cagaacgtaa gttaagcctg gagccattaa tcgctcaccg
    20401 tggaagcttt gaaagcttca cccaggcggt gcgtgacatc gctcgtaatg ccatgccggg
    20461 caaagtgttg ctcattccat gaaaccgcgg gccagcgtga tgctggcccg gtattgtgca
    20521 aaacagatca ttcaccaatg gtcccccttc gtttacacta gccacaattg aatatgggta
    20581 aatgacgatg aattcattcg agcgaaggaa taagatcatc caattagtga atgaacaggg
    20641 aaccgtgctt gttcaggatc tggcgggagt atttgctgcc tcggaagcga caatccgtgc
    20701 cgatttgcgc tttctcgaac aaaaaggcgt ggttacgcgc tttcatggcg gtgcggcgaa
    20761 aataatgtcc ggtaatagtg aaaccgagac ccaggaagtc gggtttaaag agcgatttca
    20821 gctcgccagc gcgccaaaaa acagaatagc gcaggcggca gtcaaaatga tccatgaagg
    20881 gatgaccgtt attctcgaca gcggaagtac cacaatgctt atcgccgaag gattaatgac
    20941 cgctaaaaat atcacggtga ttaccaacag tctcccggca gcgtttgccc tttccgaaaa
    21001 taaagacatt actctggtcg tctgtggtgg cacggtccgc cataaaacgc gctcgatgca
    21061 tggttccatt gccgagcgtt cattgcagga tattaatgcc gatttaatgt ttgtcggtgc
    21121 tgatggtatt gatgcggtta atggtattac gacctttaat gaaggttatt cgattagcgg
    21181 ggcgatggtc accgccgcaa ataaagtgat tgctgttctc gattcatcga aatttaatcg
    21241 tcgcggtttt aatcaggtac tgccaatcga aaaaattgac attattgtta ccgatgatgc
    21301 tgtgtcagag gtggataaat tggcattgca gaagacacgg gtaaaattaa ttacggtgta
    21361 gtattgttct gtatgccgga taaggtgttt acaccgcatc cggcattacg gattctccct
    21421 ggttcaagag agaatagttt gattaacgca ataatggaca atctggtggt aatcgacaac
    21481 gcaacgccgc cggaagtatt tcaatatgga agttttgccc actcaacggt tcgccatcaa
    21541 gattaaaagt gatttcgtgt ggggcttgta tatcaaacca cgacgaagcg ccttcgataa
    21601 tattcgggtt atcttcgtca gattttaagg ttgatacgag agccggaaga atttcatcgc
    21661 cggtaaaaat gcgcagttgc agcaagccat cgttaattaa cgcgttcggg cacaattgtt
    21721 gaccgccacc ggcctgacgc ccgttaccaa taccaatgac cagggcgtca ccttgccagt
    21781 gaaagttttc accgcggatt tcacaacggt ccggttgcag agtatccatg cgcattaagc
    21841 catgaatgat gtaagagacg ccacccagcg cggcttttaa tttttccggc gtttctgtgg
    21901 taatacgcgt cccaaatccg cctgtcgcca tattaataaa acaggtttgt ttgttgacct
    21961 gcgccatatc tatcgcaatg gcattaccgg caattgccag tttcagcgcc ttatccagtg
    22021 cctcaggaat ccctacactg gtggcaaaat cattggcggt tcctaatggc aaaattccca
    22081 gcgcgggtat gtcatccccc tcacactgaa tcaacgccgt agaaacttca ttaatggtgc
    22141 catcgccacc accggcaatc accgttgcga cgcccaactt ccgggcctcc tctacaaatc
    22201 gtgcggcatc acctttctcc caggtgaccc gcacatggat cgtcattcct tcctcacgca
    22261 acagcataat tgcttcgcgc aagggtagat tgtcagtact tttaccatta agaatcagta
    22321 agctggcggg aaattctgcc atgatcgtat gtgcctttat gattggtctg tagatagtgt
    22381 agagcagaaa acaaaaggtg gagtcagaac aggatgaaag tcggaggata aaaagtcagt
    22441 tcaagtatgg aggattcagt ggttaccacc aggagcttgc aatggggaca gcgaatacta
    22501 acggctgcca ccgataaatt tcaaaaaaga gcatatacct aatattcaac taaacagtgg
    22561 catcttcaat ataatatatt aaagccccca tggagttacc ctgaagggcc tcaatgtccg
    22621 taattcctac ttatgtagga aatgttgtac agagcattta ttataatcct attcaattat
    22681 aataatcatg ccattattat atttaaacac tagagagtgt cgttggtatt taatggggga
    22741 aggtgagatg aaaaagatag ctgctatatc attaattagt atttttctta tatctggttg
    22801 tgctgtgcat aatgatgaaa caagtatcgg taaatttggt cttgcatata aaagtaatat
    22861 tcagcgtaaa ctcgataacc aatactacac cgaagccgaa gcttctttag ccaggggcag
    22921 aatatctggt gcagaaaata tagtaaaaaa tgatgcagcc catttctgtg ttactcaggg
    22981 caaaaaaatg cagatagttg acctgaagac agaaggtgca ggattacatg gcgtcgctcg
    23041 tctgacattc aaatgtggag agtgagaata tttttttgta agcgtcaaac atgcgcgttc
    23101 tggctgtgcg tagccggaac ctgggcgagc acgataccgt tacgtgaaag gcattgtgct
    23161 atgaaggggg attctatcga tgtggtcaat ggaagacggt tatcagggat caatccttct
    23221 ttacagctta tgtacaaaaa ataagcccgt gtaagggaga ttacacaggc taaggaggtg
    23281 gttcctggta cagctagcat tttatggatt atgtttttca gcgaaacgga tgataacctt
    23341 aataaatgca gctgtatgtg atcggtttct aagaattttc catccgggaa aaataatcga
    23401 aattaatcac ttaccgtggg ggttacgcgt ggtttccccg gagaaattac gcattagcag
    23461 cgcgtaattt agctcaagat cctgcgggac cgggagccac acagtataac catcgcctgg
    23521 tgcgaccggc atagcttcgc ctttggcgtt ttccatgtgc tcaagggtaa agttaatgtt
    23581 gccttgcggc gtcatcagct caagactgtc gccaacggag aatttatttt tcaccgctac
    23641 cgctgcgagg tcccccttgc gctcaccggt aaactcacca acaaactgct ggcggtcaga
    23701 aactgaataa ccgtattcgt agttctgata atcgtcgtga gtatgacgac gcaggaaacc
    23761 ttcggtatag ccacgatgcg ccagaccttc cagagtttcc agcaggctgg tatcgaacgg
    23821 ttttcccgca gcggcgtcat cgatagcttt gcggtaaacc tgtgcggtgc gtgcgcaata
    23881 gtagaaagat ttggtacgac cttcgatttt cagcgaatgc acgcccattt tggtcaggcg
    23941 ttctacatgg gcgatggcgc gcagatcttt cgagttcatg atgtaagtgc cgtgctcatc
    24001 ttcaaacgcg gtcatatact cgcccggacg ctgggcttct tcgatcataa acactttgtc
    24061 ggttggcgcg ccgataccca gcgtcggctc aacattttgc accggaatcg gctcgtactt
    24121 gtgtacgatg ttgccaacgt catcttcttt cccttcctgg acgttgtact cccagcggca
    24181 ggcgttggtg caggtgccct ggttcgggtc gcgcttgttg atatagccag agagcaggca
    24241 gcgaccggag taggccatgc acagtgcgcc gtgaacgaag atctcgatct ccatatccgg
    24301 cacctgatta tggatctctt caatctcttc cagcgacagc tcgcgagaga ggatcacgcg
    24361 ggtcagtccc atttgctgcc agaatttcac cgtcgcccag ttcacggcgt tagcctgcac
    24421 cgagaggtgg atcggcattt cagggaagtg ctcacgcacc agcataatca gccctggatc
    24481 ggacataatc agcgcatccg gccccatttc caccaccggt ttcaggtcac ggataaaggt
    24541 tttcagcttg gcgttgtgcg gtgcaatgtt gaccacgaca taaaactttt tccccagcgc
    24601 gtgggcttca ttgatgccga gctgaagatt ttcgtggttg aattcgttgt tgcgcacacg
    24661 tagggagtaa cgcggctggc ccgcataaac agcatctgcg ccataagcga aagcgtaacg
    24721 catatttttc agcgttcccg ccggggaaag gagttccggt ttaaacataa ttctctcgtt
    24781 ctgatgacag gtcagatccg cttcacctga tgaagcggtt aggggaggcc cccactttaa
    24841 gggcgggcat tgtagcgctg taggggggat ctggtaaagc gccggacgcc aacaacccac
    24901 gcccggcatc gtaattacgt attgtctttc accgtctggc ttgtgccatt ggctttaacg
    24961 gaggcaattc cggtttcacg agattgcgcg gtggcgtaca tctggctgga gccgataatt
    25021 tgatgattag cggctttcag attgaaatag aatttgccgt tgcttgccgt tttttctcat
    25081 agcgctcttc ttgcgggctg ttgctacgca ccgacgcgat gcccttttcc gcagaggctt
    25141 ttgaggtata cagctcgctg gtgaggatag tctcaccatt gcccgctttt agcacaaacc
    25201 ggaactgatt atcactgctc ttactgagtt caaaccaacc agccataatg actccttgat
    25261 tgaaattgct tatcgaaaac agcatct
//
LOCUS       DAEEGA010000058        24603 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEEGA010000058 DAEEGA010000000
VERSION     DAEEGA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24603
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.058"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(1..374)
                     /locus_tag="KLH46_002552"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="KLH46_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            898..2826
                     /gene="thrS"
                     /locus_tag="KLH46_002553"
     CDS             898..2826
                     /gene="thrS"
                     /locus_tag="KLH46_002553"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBE0379190.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2830..3372
                     /gene="infC"
                     /locus_tag="KLH46_002554"
     CDS             2830..3372
                     /gene="infC"
                     /locus_tag="KLH46_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBE0379191.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3469..3666
                     /gene="rpmI"
                     /locus_tag="KLH46_002555"
     CDS             3469..3666
                     /gene="rpmI"
                     /locus_tag="KLH46_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBE0379192.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3719..4075
                     /gene="rplT"
                     /locus_tag="KLH46_002556"
     CDS             3719..4075
                     /gene="rplT"
                     /locus_tag="KLH46_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBE0379193.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4192..4316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4198..4242
                     /gene="pheM"
                     /locus_tag="KLH46_002557"
     CDS             4198..4242
                     /gene="pheM"
                     /locus_tag="KLH46_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBE0379194.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4525..5508
                     /gene="pheS"
                     /locus_tag="KLH46_002558"
     CDS             4525..5508
                     /gene="pheS"
                     /locus_tag="KLH46_002558"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBE0379195.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5523..7910
                     /gene="pheT"
                     /locus_tag="KLH46_002559"
     CDS             5523..7910
                     /gene="pheT"
                     /locus_tag="KLH46_002559"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBE0379196.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQHRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7915..8214
                     /gene="ihfA"
                     /locus_tag="KLH46_002560"
     CDS             7915..8214
                     /gene="ihfA"
                     /locus_tag="KLH46_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBE0379197.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8315..9295
                     /gene="btuC"
                     /locus_tag="KLH46_002561"
     CDS             8315..9295
                     /gene="btuC"
                     /locus_tag="KLH46_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBE0379198.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9358..9909
                     /gene="btuE"
                     /locus_tag="KLH46_002562"
     CDS             9358..9909
                     /gene="btuE"
                     /locus_tag="KLH46_002562"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBE0379199.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9909..10658
                     /gene="btuD"
                     /locus_tag="KLH46_002563"
     CDS             9909..10658
                     /gene="btuD"
                     /locus_tag="KLH46_002563"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBE0379200.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10736..11161
                     /locus_tag="KLH46_002564"
     CDS             10736..11161
                     /locus_tag="KLH46_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBE0379201.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRDRFDLHLPRETKQQASIGTQIDKDELLPGDLVFFKTGSGQNGLH
                     VGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11408..12121
                     /gene="ydiV"
                     /locus_tag="KLH46_002565"
     CDS             11408..12121
                     /gene="ydiV"
                     /locus_tag="KLH46_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBE0379202.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12185..13621
                     /locus_tag="KLH46_002566"
     CDS             12185..13621
                     /locus_tag="KLH46_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBE0379203.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="KLH46_002567"
     CDS             complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="KLH46_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBE0379204.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="KLH46_002568"
     CDS             complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="KLH46_002568"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBE0379205.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAIEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="KLH46_002569"
     CDS             complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="KLH46_002569"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBE0379206.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLNRRM
                     Y"
     gene            16316..18694
                     /gene="ppsA"
                     /locus_tag="KLH46_002570"
     CDS             16316..18694
                     /gene="ppsA"
                     /locus_tag="KLH46_002570"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBE0379207.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="KLH46_002571"
     CDS             complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="KLH46_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HBE0379208.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20454..20747)
                     /locus_tag="KLH46_002572"
     CDS             complement(20454..20747)
                     /locus_tag="KLH46_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBE0379209.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20744..22033)
                     /locus_tag="KLH46_002573"
     CDS             complement(20744..22033)
                     /locus_tag="KLH46_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE0379210.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22089..23027)
                     /locus_tag="KLH46_002574"
                     /pseudo
     CDS             complement(22089..23027)
                     /locus_tag="KLH46_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(23047..23811)
                     /locus_tag="KLH46_002575"
     CDS             complement(23047..23811)
                     /locus_tag="KLH46_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBE0379211.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24127..>24603
                     /locus_tag="KLH46_002576"
     CDS             24127..>24603
                     /locus_tag="KLH46_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBE0379212.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAI"
BASE COUNT         6095 a         5939 c         6464 g         6105 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt
      181 cgacagtttg cggtaaattc ctgagtaaca cagtatgaca gcatcgaact taagtcagag
      241 gtatcaagta gggtgatgag ctggcgcgca aatttattaa taggctctga tgaaggtgtt
      301 ggtatgtcgc tatcgatgtg caatctatga ggagacttat aatgagttct gataatgata
      361 tcgttttgac tcataatcta ttcgccttat atatatctgg aggttttgag gatgatgatt
      421 aattattatc gccagaacga aatgtgcacg aaaaattgtt ataagaattg tgtcactttt
      481 atcattgtga tatgaccact tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa
      541 atcacgcaac gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac
      601 ctttcagacg caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact
      661 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      721 tgttaacatc gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      781 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      841 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      901 cctgttataa ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg
      961 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
     1021 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
     1081 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat
     1141 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
     1201 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
     1261 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     1321 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtttc cattcttgac
     1381 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1441 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1501 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1561 gcgtgggcag acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1621 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     1681 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     1741 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1801 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct
     1861 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1921 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     1981 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag
     2041 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     2101 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     2161 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     2221 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     2281 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     2341 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     2401 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggagcgtttc
     2461 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag
     2521 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2581 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2641 atccgcgagc acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg
     2701 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2761 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2821 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2881 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2941 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     3001 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     3061 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     3121 ttccgtccta gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     3181 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     3241 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcggta
     3301 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     3361 aagaaacagt aaggccttca agtagcacta tctgtggagc ctttgggctt cacaggcatt
     3421 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3481 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3541 aagcacgcta acctgcgtca cattctgact aaaaaagcga ccaaacgtaa acgtcacctg
     3601 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3661 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     3721 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     3781 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     3841 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     3901 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     3961 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     4021 cgacaaagta gcgttcacca ctctggttga aaaagcgaaa gcagctctgg cataagccag
     4081 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     4141 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     4201 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     4261 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     4321 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggacac
     4381 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4441 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     4501 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     4561 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
     4621 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4681 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4741 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4801 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     4861 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4921 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4981 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     5041 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     5101 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     5161 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     5221 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgag
     5281 ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggctgc
     5341 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     5401 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     5461 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5521 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgata
     5581 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     5641 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5701 acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgcg
     5761 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc
     5821 cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt
     5881 gctccttttc tgagctggga atttctgacg atcacagtgg cattatcgaa ctgcctgcgg
     5941 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgataac accatcgaaa
     6001 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg
     6061 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     6121 acgacacgtt gccgattaca gtcgaagcgc cggaagcctg cccgcgctat cttggccgtg
     6181 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     6241 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac
     6301 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6361 tggcaaaaga gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg
     6421 acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg
     6481 aacactctgg cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc
     6541 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6601 atgagcgtgg cgttgatccg gtactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     6661 tgatcgacat ctgcggtggt gaggctggcc cggtaattga tatcaccaac gaagcaacgc
     6721 tgccgaagcg tgcaaccatc actctacgtc gtagcaaact ggatcgcctg ataggccatc
     6781 atattgctga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6841 gcaaagacga atggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag
     6901 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     6961 aggcaagcct gattatgggg actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa
     7021 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     7081 tgcagcagat gatccatcca ggcgttgaag ccttactgct ggcaagcccg atctctgttg
     7141 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     7201 agcaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     7261 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7321 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7381 accttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7441 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7501 gttttgttgg agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     7561 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7621 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7681 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7741 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7801 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7861 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7921 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7981 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     8041 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     8101 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     8161 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     8221 ctaactaaaa aggccgctat gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     8281 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8341 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8401 aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc ctcgtggcga
     8461 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8521 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8581 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8641 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
     8701 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     8761 cgttgcatta gggattatct gcagcgcact aatgacgtgg gctatctact tttccacctc
     8821 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8881 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     8941 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccatgtg
     9001 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct
     9061 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
     9121 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     9181 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     9241 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9301 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacctagg agaaacgatg
     9361 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
     9421 aagtacgccg gtaatgtgct gttaattgtc aatgtcgcct caaagtgtgg cttaacgccg
     9481 caatatgagc agttggagaa tattcaaaaa gcctgggccg atcgaggttt tgtggtgctg
     9541 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     9601 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     9661 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg
     9721 gaagagagcg gattctatgc ccgtatggtc agcaaaggcc gtgcaccgct gtacccggat
     9781 gatattttat ggaattttga aaaattcctg gttggcaggg acggaaaagt catccagcgt
     9841 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     9901 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     9961 cgctttcagg cgaggttcgg gctggggaaa tcctgcactt ggtggggccg aatggcgcgg
    10021 gtaagagtac cttactggcg caaatggccg gaatgaccag cggtaaggga agcattcagt
    10081 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc
    10141 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
    10201 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
    10261 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    10321 ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg
    10381 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    10441 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacgttgc
    10501 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    10561 aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    10621 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cccgaaaatc
    10681 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
    10741 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
    10801 gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct
    10861 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg atcgtttcga
    10921 tttgcatctg ccccgtgaaa ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga
    10981 cgagttgctg cctggtgacc tggtcttttt caaaacgggt tccggacaaa atggtttgca
    11041 tgtaggtatt tacgatacca acaaccaatt tatccacgcc tctaccagca agggagtgat
    11101 gcgttcctca cttgataatg tctattggca gaaaaatttc tggcaggcga gacgaatcta
    11161 gtatgtcgaa aaattgacgg cgatgataca tagccgtcaa tttactcctg aatacagtag
    11221 tattttgcag acaaaaattc cgtaaatgga ttattcgttt ataatcagga taatctaaaa
    11281 aaaggccccc tgaaaaagtg gttaacatgg aacaaatgaa agttactcct tctgtaattg
    11341 ttatgtctta tggcctcatc ccttttagcc ggatactgaa aaacatcctt cgagagggac
    11401 ggttaccatg aagatttttt tggagaatct ttatcattct gattgctatt tcctgccgat
    11461 aagagataac cagcaactcc ttgtcggcgt tgagttaatt acccatttct ccagtgaaga
    11521 tggcacggtt cgtatcccga cgagtcgggt tatagcgcaa cttaccgagg agcaacattg
    11581 gcaattattt tcagagcagt tggaattact aaaatcatgc caacactttt ttattcaaca
    11641 taaactcttt gcctggctta atttaacgcc acaagtggct acattattat tagacaggga
    11701 taattacgca ggcgaactat taaaatatcc gtttattgag ctgttgatta atgagaatta
    11761 cccgcatctc aatgaaggaa aagataatcg cgatcttttg tccttatcgc aaatgtatcc
    11821 ccttgtgtta ggcaatttgg gagcaggtaa tagcacaatg aaagcggttt ttgatggtct
    11881 tttcacccgc gtaatgctgg agaaaggttt tattcagcaa caaatcacgc atcgctcctt
    11941 cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt gcatcatcgc
    12001 ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt tccatgctct
    12061 ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg ttcagcgata
    12121 accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca aacagatagg
    12181 atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc
    12241 actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg aactcgctaa
    12301 cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac
    12361 cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt tcgtcgtctg
    12421 ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc ttgctgatgg
    12481 cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga
    12541 tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg cgatgcatta
    12601 tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg
    12661 ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg
    12721 tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt tggctgactt
    12781 tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc gtctctggtt
    12841 taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc
    12901 acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg attacgggcc
    12961 gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg atcatcaagg
    13021 gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc gtctggcgca
    13081 gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca gctatcagca
    13141 ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca tgacggagca
    13201 aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag agcgcagcga
    13261 ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct
    13321 acgtgaggag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg
    13381 tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc
    13441 agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag aaaagggtga
    13501 tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg acagagatga
    13561 tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct gctcgagtta
    13621 aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga tattcttgcc
    13681 cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg gtaattcgcc
    13741 ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc
    13801 tatccgtata acgcataaaa attaataata attgatataa caatgatttc tattatcaat
    13861 attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg cgcagaaatg
    13921 cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa aggcgagttt
    13981 ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta
    14041 agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat
    14101 aattccagca atctccgtag agccattgcg gatttgctga caaatatcct cacaaacttc
    14161 taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca ccagatgttc
    14221 aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg catgataatt
    14281 cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat
    14341 ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat
    14401 agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta ccggacagga
    14461 gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc caatcgcgcc
    14521 ccaactgatt aaatcagcaa taaactgacc ggttaccata tcgaggaact cagtcgcggt
    14581 agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt
    14641 tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc cgacaacagt
    14701 tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt actggttgcg
    14761 cagcgactgc agacgggtgg cgtactctat tgcagcggtg agatcgtgga tcgagcaggg
    14821 gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa ttctgcggcg
    14881 ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg cgagttcggc
    14941 gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag
    15001 tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca tcacgataaa
    15061 ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat
    15121 aacaaaaaaa caaaccaatg cactagttct ctagtacatt cggcgattaa ggcccatgat
    15181 atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat
    15241 ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact gacgaagcga
    15301 ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat
    15361 agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca ccagattatc
    15421 catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca ttgccagata
    15481 cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct gagcctgatc
    15541 cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg
    15601 cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt
    15661 tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca
    15721 aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg agtagaagac
    15781 cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc
    15841 acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg gaaattgtga
    15901 cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac
    15961 gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata attctatgaa
    16021 agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata aaatgaacct
    16081 tcattttatt ttttataaaa acagcatttc atttttatgg tttcgtttat accgatggtt
    16141 tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc ttaaaaagta
    16201 aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggttaaat atgcaaagat
    16261 aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc
    16321 caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga atgatgtaga
    16381 cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt ccggaatggg
    16441 tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt ttctggacca
    16501 aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg acgatgttac
    16561 tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc ccttccagcc
    16621 tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg acgaaaacgc
    16681 ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt cctttgccgg
    16741 tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca
    16801 tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc agggttacga
    16861 tcaccgtggc gtggcgctct ccgctggtgt tcagcggatg gtgcgctccg acctcgcatc
    16921 ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg tgtttatcac
    16981 ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg atgagtttta
    17041 cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca ccatggggtc
    17101 gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga
    17161 agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag tgcaggaact
    17221 ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata ttgagtgggc
    17281 gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc
    17341 acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta tcgccgaagg
    17401 ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg acatcagcga
    17461 aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc cggactggga
    17521 accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc
    17581 ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag atgcaacaga
    17641 acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt
    17701 ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga tgccggatct
    17761 gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact tcgcctgcct
    17821 gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt
    17881 ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa acgaaatccg
    17941 cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc tgactgaagg
    18001 gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc tctctgattt
    18061 taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag atgaagagaa
    18121 cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt
    18181 cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga ccaacgttga
    18241 gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg aagaactggc
    18301 gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt gtgaaatccc
    18361 gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct caattggctc
    18421 aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg tgtctgaatt
    18481 gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc gtgccgcgaa
    18541 gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg aagactttgc
    18601 cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca ccgtggtgca
    18661 aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg cgttcagtcg
    18721 ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga catcctgcgt
    18781 taatcgccgt ataatatctt ttcttaacaa aaacttttgt attttatctg aggcagtgcg
    18841 cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga cccgtttacg
    18901 gctaaaaaaa gccgccatct cttccagcga taatgaatga tgcggcgctt tcagcacgac
    18961 ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac aggcatcgtg
    19021 aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat tttcgccgcc
    19081 gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct catccatacg
    19141 gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg ttaattcagg
    19201 ttcatcaaaa taccccataa acacattggg gccacgcgat gcttcttcac cttcgcaacc
    19261 tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg cggcagcgta
    19321 accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg catgcggcga
    19381 actttctgtg gaaccataga cacttaataa tttaatgctg cgctgctggc attcgcgcgc
    19441 cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg aaaggtctgc
    19501 gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc ctagcataca
    19561 ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa
    19621 cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc ccgttgcgtg
    19681 accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc gcgcgcaata
    19741 agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg gcagaccctc
    19801 ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta tcgccgtggt
    19861 cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg cgggagccag
    19921 tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg gcaggattaa
    19981 atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg cctgacactt
    20041 attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca cggaaaccgc
    20101 accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag gcagttgaaa
    20161 tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca gacagctcgc
    20221 ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca cggcaatttt
    20281 gtctggcatc gcacgagcgg tctgctgcca gtaatcggcc agtgaagcat cgccccatag
    20341 cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca ctttcatttt
    20401 ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca tgattagccg
    20461 tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag aatagtgtta
    20521 ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa atgaatgttt
    20581 cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt acggaattca
    20641 ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac atggaattta
    20701 ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc gcggttgctc
    20761 ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt cccatgatca
    20821 ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc atgatgtcgg
    20881 ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc gggatcttgc
    20941 gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg tattgcgcca
    21001 gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg gcggcagcct
    21061 gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc aggcagaagc
    21121 ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc attgccagac
    21181 cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta atcagcgggc
    21241 gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg
    21301 cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg gtatagagaa
    21361 atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg gcaccttcct
    21421 cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata acctctttaa
    21481 caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag cggccaagca
    21541 tcgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca tccccagcct
    21601 gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg cctgggataa
    21661 actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga ttacgcaata
    21721 cggtataaga tgcgtgttga ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg
    21781 cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct accggcgctg
    21841 atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca
    21901 tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc ccggctcgtg
    21961 ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg gcatcaaatt
    22021 tgtcatccga catgcgttat gctcccttcc tgctgcgttt acagcgggag atgtggttga
    22081 aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta gatatcgccc
    22141 accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt gatggcgaca
    22201 atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat ccccagcgtc
    22261 aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc acgctccatc
    22321 cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag caccgccgcc
    22381 agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac gccaaccaca
    22441 cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc gcgacagaga
    22501 atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg agaggtatca
    22561 ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg gctgttgatc
    22621 ttttcctaag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat gtgaccatcg
    22681 acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac acttaaccgt
    22741 gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg gtgtttatct
    22801 ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc gtcgttttgc
    22861 tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg gtcggtattt
    22921 tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag ttcagcataa
    22981 cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt ttcttctccg
    23041 taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg cctccggcga
    23101 atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa tgccgaccag
    23161 ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact gatttaccgg
    23221 ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt taatatcgga
    23281 ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat cttcaagcgt
    23341 gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat taatcaccgg
    23401 aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt cgccggaacc
    23461 ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca gcgcttttgc
    23521 ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca ggctgtgcgg
    23581 cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc cgccaacggt
    23641 cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa tggcattgag
    23701 atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac
    23761 aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca tgatttctcc
    23821 tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc
    23881 gatagcggtg cggggggttc gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa
    23941 aaaatgcgct tgctaaaaca aaagtgcaat ttgcggcaat atagttattc catgaccgta
    24001 caatcttgca ttcagtgtaa ataatcttgt ttagctctcg ctaaacgcgg ttgtataacc
    24061 gcaatacacg ccgtatttta tccggcatat attgtttttg cttgctcatg gaatagggag
    24121 gttgtcatgt atcaacgctg ttttgataat gccagtgaaa cgctgtttgt cgccggtaaa
    24181 acgccacggc tttcacgttt tgcatttagc gatgatccaa aatgggagtc tggacatcac
    24241 gttcatgaca atgaaaccga gctgatttac gtcaagaaag gggttgcaag gtttaccatc
    24301 gattcttcgt tatatgtcgc acatgcggat gacattgtgg tgatagaacg cggcaggctg
    24361 catgcggtgg cctctgacgt taacgatccg gcaacgacgt gtacctgtgc gctgtacggc
    24421 tttcagtttc agggggctga ggaaaatcag ttactgcaac cgcattcttg tccggtaatt
    24481 gccgcggggc agggaaaaga agtcataaaa accttattta atgagctaag tgtgattttg
    24541 ccgcaaagta aaaatagcca aacatcttcg ttatgggatg catttgccta tacattagca
    24601 att
//
LOCUS       DAEEGA010000059        24183 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEEGA010000059 DAEEGA010000000
VERSION     DAEEGA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24183
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.059"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(25..597)
                     /locus_tag="KLH46_002577"
     CDS             complement(25..597)
                     /locus_tag="KLH46_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBE0379213.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="KLH46_002578"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="KLH46_002578"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBE0379214.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="KLH46_002579"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="KLH46_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBE0379215.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="KLH46_002580"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="KLH46_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBE0379216.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="KLH46_002581"
     CDS             complement(3559..5340)
                     /locus_tag="KLH46_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBE0379217.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="KLH46_002582"
     CDS             complement(5333..7105)
                     /locus_tag="KLH46_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBE0379218.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="KLH46_002583"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="KLH46_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HBE0379219.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="KLH46_002584"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="KLH46_002584"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBE0379220.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRECDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8664..10356
                     /locus_tag="KLH46_002585"
                     /pseudo
     CDS             8664..10356
                     /locus_tag="KLH46_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10421..11116
                     /gene="queC"
                     /locus_tag="KLH46_002586"
     CDS             10421..11116
                     /gene="queC"
                     /locus_tag="KLH46_002586"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBE0379221.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11168..11566)
                     /gene="fadM"
                     /locus_tag="KLH46_002587"
     CDS             complement(11168..11566)
                     /gene="fadM"
                     /locus_tag="KLH46_002587"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBE0379222.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11660..12031)
                     /locus_tag="KLH46_002588"
     CDS             complement(11660..12031)
                     /locus_tag="KLH46_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBE0379223.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12182..14053)
                     /gene="ppiD"
                     /locus_tag="KLH46_002589"
     CDS             complement(12182..14053)
                     /gene="ppiD"
                     /locus_tag="KLH46_002589"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBE0379224.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14245..14517)
                     /gene="hupB"
                     /locus_tag="KLH46_002590"
     CDS             complement(14245..14517)
                     /gene="hupB"
                     /locus_tag="KLH46_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBE0379225.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14726..17080)
                     /gene="lon"
                     /locus_tag="KLH46_002591"
     CDS             complement(14726..17080)
                     /gene="lon"
                     /locus_tag="KLH46_002591"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBE0379226.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17268..18542)
                     /gene="clpX"
                     /locus_tag="KLH46_002592"
     CDS             complement(17268..18542)
                     /gene="clpX"
                     /locus_tag="KLH46_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBE0379227.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18668..19291)
                     /gene="clpP"
                     /locus_tag="KLH46_002593"
     CDS             complement(18668..19291)
                     /gene="clpP"
                     /locus_tag="KLH46_002593"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBE0379228.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19538..20836)
                     /gene="tig"
                     /locus_tag="KLH46_002594"
     CDS             complement(19538..20836)
                     /gene="tig"
                     /locus_tag="KLH46_002594"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBE0379229.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21180..21497)
                     /gene="bolA"
                     /locus_tag="KLH46_002595"
     CDS             complement(21180..21497)
                     /gene="bolA"
                     /locus_tag="KLH46_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBE0379230.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21802..22380
                     /locus_tag="KLH46_002596"
     CDS             21802..22380
                     /locus_tag="KLH46_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE0379231.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22424..23899
                     /gene="ampG"
                     /locus_tag="KLH46_002597"
     CDS             22424..23899
                     /gene="ampG"
                     /locus_tag="KLH46_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBE0379232.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVMLGGLLDYLALRKTHLT"
BASE COUNT         5664 a         6666 c         5829 g         6024 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacgg taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatcgcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcgaaag tgagggtaat
     8461 gtcgcattca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taataatatt gttccgggtt tatatgcgat tgctcaaccg tcttaaccag tatcaacgtc
     8701 tgtggcaacc ttcagccgca aaccgtcacc gtcagcgaac tggccgaacg ctgtttttgc
     8761 agcgaacgcc atgttcgtac gctgttgcgt caggcacagg aggcgggatg gctggagtgg
     8821 caggcgcagt caggacgcgg aaagcgcgga caattacgct ttctggtcac gccggaatcg
     8881 ctacgcaatg cgatgatgga acaggcactg gaaaccggaa agcagcaaga tgtgctggag
     8941 ctggcgcaac tggccccagg tgagctgcgc actctgttac agccatttat gggcggacaa
     9001 tggcaaaacg atacacccac gttgcgtatt ccctactatc gcccgctcga accgctacaa
     9061 ccaggctttt tgcccggccg tgccgagcag catctcgccg ggcagatatt ttccggcctg
     9121 acccgcttcg ataataatac tcagcgcccg attggcgatt tagcgcatca ctgggaaacc
     9181 tctactgaca ggttacgctg ggacttttat cttcgttcaa ccctacactg gcataacggc
     9241 gatgcagtaa aagcctcaca cttacaccag cgattattga tgctgttaca actgccagca
     9301 ctggatcaat tatttattag cgtgaagcgt attgaagtca cccatccgca gtgtctgacc
     9361 ttctttttac atcgccccga ttactggctt gcgcaccggc tggcgagcta ttgcagccat
     9421 ctggcgcatc cgcaattccc actgatcggc actggtcctt ttcgcttaac acaattcaca
     9481 gcggagctgg tgcgcctgga gagccatgat tattaccatt tgcgtcatcc gctgcttaaa
     9541 gcggttgagt actggataac tccgccgctt ttcgaaaaag atttgggaac cagttgtcgg
     9601 catcccgtgc aaatcaccat cggcaaaccg gaggagctgc aacgggtcag ccaagtcagt
     9661 agtggcatca gtttaggttt ttgctattta acgttgcgca aaagtccccg actctccctc
     9721 tggcaggcgc gaaaagtgat ctccattatt catcaatccg gtttattaca aacgttagaa
     9781 gtcggagaaa acctgatcac cgccagtcat gcattactgc taggctggac tattccgcac
     9841 tggcaagtac cggatgaagt caaactaccg aaaaccttga cgctggttta tcacctaccg
     9901 atagaacttc ataccatggc agaacgccta caggcgacac tggcagcgga aggctgtgaa
     9961 ctcacaatta tttttcataa cgcaaaaaac tgggacgaca cgaccctact ggcacacgca
    10021 gacctcatga tgggcgacag attaattggc gaagcaccgg aatatactct ggagcaatgg
    10081 ctacgttgcg atccactatg gccacatgtt ttcgacgctc cagcatatgc acatctgcaa
    10141 tcgacgctgg acgcggtgca agtaatgcct gatgaggaaa accgatttaa tgccctgaaa
    10201 gcggttttta gccagttaat ggcagacgcg acgctgacgc cgctgttcaa ctatcactat
    10261 cgcattagtg cccctcccgg cgtgaacggt gtgcgactga caccgcgcgg ctggtttgaa
    10321 tttaccgaag cctggcttcc cgcgccgtcg caatgaatag ctggtccggg ttaatcgcag
    10381 gcgttaccat aacgctttta ttgttcaagc aggattatct atgaaacgtg ctgtcgttgt
    10441 gttcagtgga ggccaggatt ccaccacctg tctggtgcag gcattacaac aatatgatga
    10501 agtccattgc gtgacgttcg attacggtca gcgacatcgc gcagaaatcg acgtggcacg
    10561 cgaactggcg ctgaaactgg gagcacgcgc gcataaggtt cttgatgtaa cgctgctcaa
    10621 cgagctggcg gtcagcagcc tgacacgtga cagcattccg gtgcctgatt atgaacctga
    10681 agccgatggc atcccgaata cgtttgtccc agggcgtaat attttgttcc tgacgctggc
    10741 ggcaatatat gcgtatcagg taaaagcaga agccgtaatt accggcgtct gtgaaacgga
    10801 tttctccggc tacccggatt gccgcgatga gtttgtgaaa gcactaaacc atgccgtcag
    10861 tttgggcatg gcgaaagata ttcgttttga aacgccgctg atgtggattg ataaagcgga
    10921 aacctgggcg ctggcagatt attacggcaa actggattta gtccgtaacg aaacgttgac
    10981 ctgctataac ggcattaaag gcgacggttg cggtcattgt gcggcatgta atttacgcgc
    11041 caacggtttg aatcattatc tggccgataa accgacggtg atggcagcga tgaagcagaa
    11101 aaccgggttg aggtaattat tccgggtgtc gccggatgcg gcttgagcat ccggcaccac
    11161 aaaacgttta cttaaccatc tgctccagct tttcgcgcaa ttccccttcc agagctaatg
    11221 ctttctgcgt tttaagatca atacaaacaa acgtaataag cgcatccgct accacctgcc
    11281 cttccggctc cagtgtaatg acctggttta agatgccgct tttaccgttt aattgctgca
    11341 actgactggt aatagttaac aggtcgctta ataccgctgg gcgacgatag ttaatattga
    11401 tattgaccac gacgaaggcg atgttatggg ccgtcatcca ctgaaaactg tcgctatttt
    11461 ccaccccatc ccagcgggct tcttcgagaa attcaaggta gcgggcgttg ttgacgtgct
    11521 ggtaaacgtc gagatgatat ccacgaactt tgatttgtgt ttgcatagcg caataacctt
    11581 acgttgttgt tataagtaca gaggtcataa ctggtatgac ctcttcagtc tggcaaaaaa
    11641 ttgccactat gcaaattaat tacagggtta ataccgccag attacgttcc accagcgaat
    11701 tgcccatccc cggcacctgc tttagatcct ccaccgtttt aaacggaccg tactcttcgc
    11761 gataactgac aatcgcctgc gctttcttca ggccaacgcc attcatcgcg cgggctagct
    11821 cttccgcgct ggcattatta atgctgaccc gggtgccttc ttcgtcactg gctttcgccg
    11881 gtactgctgc tttactttgt gctgcaggag cttccgcttt ggtttctacc gccgtcggtt
    11941 tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc agggacaggg
    12001 taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg ttaacagtgg
    12061 aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa tggaaaaggc
    12121 cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg cggcttcagg
    12181 attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc aggttactca
    12241 tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc atcgcttttt
    12301 tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga accacattac
    12361 cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt ggcagtgcaa
    12421 acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc tcgccaaatt
    12481 tcagaccggc agcctgcata gcttccgcac ctttgccggc tttcaaatca accagcagtt
    12541 tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt gccttaactt
    12601 gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg acgcgcagca
    12661 cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccgac gcgccgtttt
    12721 cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc aactcttccg
    12781 gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca gcttgctctg
    12841 caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc tgcagcgcat
    12901 agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca cgtacttcgt
    12961 ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg aaaccgaccg
    13021 aagatttgat gacaccagac agttggcctt tttctttcag accagcattt ttcagttcgt
    13081 cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca gagataatat
    13141 cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc tcatcaagta
    13201 ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta cgctgcggct
    13261 gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg ctaaccggtt
    13321 gctgcatcgt tgcggcaacc agcttgatgt aactcacgcg gaattgttcc ggcgtcatga
    13381 aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca ggctgcttcg
    13441 ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc gcgaccagtg
    13501 ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaacg ccgttaatca
    13561 gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg gtcatcccca
    13621 tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc tggaaggctg
    13681 gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc agctcacgag
    13741 cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga cgcagggttt
    13801 tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt tgctgcatac
    13861 gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc tggtcattca
    13921 cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg gtcaatatga
    13981 acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca gccgtgcgta
    14041 agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct cacgcaacgt
    14101 atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag cccacaagac
    14161 acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc ttgaacttcg
    14221 tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt tacctgcacg
    14281 gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg ggttgcggcc
    14341 agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg ctacatcatc
    14401 cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac ggccagccgc
    14461 agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt tattcactct
    14521 tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa cgcgcagcag
    14581 ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc cgatccgcta
    14641 tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca gccctgtttt
    14701 tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg ccagacggtt
    14761 cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga atgtccagat
    14821 cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac ggaattagca
    14881 ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca ccgatcggca
    14941 gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga accgggttac
    15001 cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga ccatctttcg
    15061 gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa tcagggttga
    15121 tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata gactcctgca
    15181 tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca catgcggttt
    15241 caatggtcag caagtcaccg cccacttccg tccacgccag accgattacc tgaccgacac
    15301 ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag tcatgcaggt
    15361 tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc ttaaccgctt
    15421 tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca cgggtgtagt
    15481 aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt ttcagtgcat
    15541 tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt tcatcttcgg
    15601 tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga atgttcatgg
    15661 agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc aggtagtggt
    15721 cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag gccggatcgc
    15781 ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcgga tttttcacgc
    15841 ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa gtacggcggt
    15901 gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca tatttacgcc
    15961 cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc ggccctacca
    16021 ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg tactcaagga
    16081 tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc tgcgcctgac
    16141 gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc cagtcgatat
    16201 aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc ttctgcaact
    16261 ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt ttgcgcttca
    16321 gggcttcgtt ttcgtccggc acgtcgtcca tttcaccgag ttctttctga atagctttca
    16381 tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta acgcggttgc
    16441 gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc atcagatatt
    16501 ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc agtttcagcg
    16561 gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta ttcagcgacg
    16621 tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac tggctgattg
    16681 cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc agatactccg
    16741 ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg cgctgtaacc
    16801 cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat atagaggcca
    16861 cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct tctttctgcg
    16921 cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg atagattttt
    16981 cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg cgcagcggca
    17041 atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct cttagtttaa
    17101 tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa catgtacgtc
    17161 agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa ttaaagggga
    17221 gataaaatcc cccctttttg gttaactaat tgtatgagaa tggttaatta ttcaccagat
    17281 gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc atcaattacc
    17341 gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta catggtatcg
    17401 agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg cgccatcgct
    17461 ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc ttccagatta
    17521 aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat ctgaatcaga
    17581 gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat gaactcaggg
    17641 ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc gccttcgctt
    17701 gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc ggtttctaca
    17761 cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa caggatctta
    17821 gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg cggtggaaca
    17881 gcagctaccg taccttcgat cagtttcaac agtgcctgct gtactccttc accggaaacg
    17941 tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc atcgatgtag
    18001 acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag cttctgaatg
    18061 atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc gtcggccatg
    18121 gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt accggaaccg
    18181 gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct ggtatcgccg
    18241 ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt cgcctgttcc
    18301 tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg tagcgcactg
    18361 cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt acataaatca
    18421 acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac ttcatgctgg
    18481 cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg tttatctgtc
    18541 atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa tgccattctc
    18601 aggcgcaagc cgcttacagc gttgtgccgc cctagataag tatagcggca cagttgcgcc
    18661 tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc accgcttcag
    18721 gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat gattgacccg
    18781 tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca cgggcatgaa
    18841 tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc atcacgcgcg
    18901 aattcggcag gcaaaaacgt ttaccttttg cccctgcggt caacaagaaa gcgcccatcg
    18961 aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc atggtgtcat
    19021 agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga tagatatctt
    19081 tttctgggtt ttccgcttcc aggaacagca tctgcgccac aatcaggtta gccatgtggt
    19141 cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa tagatatcaa
    19201 aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc atatggggtg
    19261 caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa ttgaaaaaaa
    19321 cctgctgtac cgattgtaac cttatggacg gattatcagc tagtccctgt gttatgggta
    19381 cggcatcacc atatttcaag cataacaatc ttttgttgtt acgctaacac cgaaagaggc
    19441 ttttcgcacg gctccatgca aaattcatga caaaaaaaag cccaccacct ggcggtgacg
    19501 ggcctttgtg cgaatttagc gcgttatgct gcgtaaatta cgcctgctgg ttcatcagct
    19561 cgttgaaagt ggtttctttt tcagtcactt tcgctttcgc cagtacagct tcaacagcct
    19621 gttcttccag agcaacattg cgcatgttgt ccatcagttc tttgtttttg ctgtagaact
    19681 cgataacttc tttcggatct tcgtacgcag aagccatctc ttcgatcagg cctttcacgc
    19741 gctcttcgtc agctttcagc tcgttggtgc ggataacttc gcccagcagc aggccaacaa
    19801 ctacgcggcg tttagcctgt tcttcgaaca gttcgcgcgg cagttccaga gcttgttttt
    19861 cgttgccacc gaaacgctgt gcagcctggc gacgcagaac gtcgatttcg ctgtcgatca
    19921 gcgcagccgg tacgtcgatg tcgttagctt ttaccagacc ttcgatcgcc tgagacttaa
    19981 cgcggttacg gatggcgctc ttcagctcgc gctccatgtt tttacgcact tcagcgcgca
    20041 gaccttctac ggaaccatct tcaacgccga aacgtttgat gaattctgca gtcagttccg
    20101 gcagttcacg ctcttcaact ttcttcaggt tgatagcgaa tttcgctgct ttacctttca
    20161 ggttttctgc gtggtattct tccgggaagg tcacgtcgat ggtgaactct tcgccagctt
    20221 tgtggccttt gataccgtct tcaaagcccg ggatcatacg accctggccc atcgccagta
    20281 cgaaatcaga cgctttaccg ccttcgaact cttcgccgtc tacagaaccg gtgaagtcga
    20341 tggttacgcg gtcttctgct tcaacagcgc cgtctttttc tttccaggtc gcctgctgtt
    20401 tacgcagagt atccagcatg ccgtcaacgt cagcgtcggt cacttcaacg atcggttttt
    20461 caacttcgat cgcttccaga ccctgcagtt caacttccgg ataaacttca aactctacag
    20521 agtaagtgaa gtcttcaccc agcttgtatt cgcccggaac ataagtcggt gcgccagccg
    20581 gattgatttt ttctttaatg atggcgtcaa tgaagttacg gctcatcagg tcacccagaa
    20641 cgtcctggcg tacagacgcg ccataacgct gagcaacgat attcattggc actttgcctt
    20701 tgcggaagcc gtcaatacgt acttttttcg caacgttgac cagctcgctt ttaacagcgg
    20761 tctcgatgct gtcagcagcg atagtaatcg ttacacggcg gccaaggcct tgagtggttt
    20821 caactgaaac ttgcatcttg ttacctcaaa aaatcacagt gctcggtcaa ctcttacccc
    20881 gcaagcacag gttgttggct tcgcggaacc gggatgttct tataatcaat cacattccct
    20941 gttaccagaa tcatcccgaa agcattcaaa taggacgcgg cattatagcg gcatcacttt
    21001 tatgagtcga gaacggttat tgcgcgctgt cgcgtatttt ttcacaattc ccgggcaatt
    21061 ttcgtctgaa tgctgggcaa aaagcgcaaa atccagacaa aacaaaacgg cccgcaggcc
    21121 gtttttcata aaatctttag caacatactg gaaaagcgcc gacagttgca aatgcgtttt
    21181 tacgcgatgc ttcctgctcc acgacaggga ggagaggcaa agacggtgtc ctgcaaccct
    21241 tcccactcct taatagtgta agtatgcaga gccagcgcat gaacggtagt agagagttcc
    21301 tccgctaaag tactgtaaat cattcgatga cgattcagaa aacgttcacc cgtaaaacga
    21361 tcgctgacca gcacaacttt aaaatggctt tcagagccgg ctgggacatt gtgacgatag
    21421 ctttcatcca ctacttcgag gaatacgggt tggaacgccg cccttaattt ttcttctatc
    21481 cgctcacgta tcatcatgaa atatctcctc cgacaacgct gagatgtcat ccatcccttt
    21541 aaatactagc cgcttttacc gtttccattg cagcttaaca acaaatattt aggtttcgtc
    21601 ctggtttttc cgtcaagcgt atgaagttta ctgatttaaa gattgaaaca caggcctcgc
    21661 atcagcgttt cttttaccag gggtgattat ttcgtcaaca tgatgaattt acatgcctta
    21721 cccacttccc ctcgccgttg gcaatgttat gatggcggga atttttacca aacacgctta
    21781 agagtctctg agagcccgaa catgttcaaa aaaatcctct tcccgttagt tgctctgttt
    21841 atgcttgcag gatgcgcaaa accgccaaca actattgaag tttccccgac gattacactg
    21901 ccacagcagg atccaagcct gatgggcgtc accgtaagca ttaatggtgc cgatcagcgt
    21961 accgatcagg cgctggcaaa agtcacccgc gataatcaaa tcgttaccct gaccgcctcc
    22021 cgcgctctgc gtttcctgct gcaagaagtg ctggaaaaac agatgaccgc gcgtggttac
    22081 atggttggtc cgaatggccc ggttaatctg caaatcatcg ttagccaact gtatgctgac
    22141 gtgtcccagg gcaacgtgcg ctacaacatc gctaccaaag cggatatcgc catcatcgct
    22201 accgcgcaga atggcaacaa aatgaccaaa aactatcgtg ccagctacaa cgttgaaggt
    22261 gcgttccagg cctccaacaa aaatatcgcc gatgcggtta acagtgtgct gaccgatacc
    22321 atcgctgata tgtctcagga caccagcatt cacgaattca tcaagcagaa cgcgcgttaa
    22381 tttctgccct ctggcccggt gcaagccggg cctgtagacg cccatgtcca gtcaatattt
    22441 acgtattttt caacagccgc gttcagccat attgctgatc ctgggttttg cttccgggct
    22501 accgctcgcc ctgacatccg gcaccttaca ggcctggatg acggtcgaaa atatcgatct
    22561 caaaaccatt ggtttcttct ctctggtagg ccaggcttac gtttttaaat tcctctggtc
    22621 accgctgatg gaccgctaca cgcccccatt ttttgggcgg cggcgcggtt ggctgctcgc
    22681 cacgcaaatc ctgttattag tcgccattgc ggcgatgggt tttctcgaac caggcaccca
    22741 actccgctgg atggcggcgc tggcggtggt gatcgctttt tgctctgcct cccaggatat
    22801 tgtcttcgat gcgtggaaaa ccgatgtgct cccggcagaa gaacgtggtg cgggcgcggc
    22861 aatcagcgtg ctgggttacc gtttagggat gctggtttcc ggcggcctag ccctgtggct
    22921 ggcagataaa tggttgggct ggcagggcat gtactggttg atggcggcgc tgttgatccc
    22981 ctgtattatc gcgacgttgc ttgcaccaga accaaccgac accattcctg tgcccaaaac
    23041 gctggaacaa gcggttgttg cacctctgcg agatttcttt ggtcgcaata atgcctggct
    23101 tattttgctt cttatcgtgc tgtataagct gggcgacgca ttcgccatga gcctgacaac
    23161 cacgtttttg attcgcggcg tcgggtttga tgcgggtgaa gtaggcgtgg taaacaaaac
    23221 gcttggctta ctggcgacca ttgttggcgc attgtacggt gggattttga tgcagcgcct
    23281 gtcgctgttc cgggcgctgc tgattttcgg cattttacaa ggtgcgtcta acgctggtta
    23341 ctggctgctg tcgattactg ataagcatct ctacagcatg ggcgcagccg tctttttcga
    23401 aaacctctgt ggcgggatgg gcacatcagc ctttgtcgcg ctgttaatga cgctgtgtaa
    23461 taagtcattt tccgctactc aatttgccct gctctcagcg ctttccgctg tagggcgagt
    23521 ttatgtcggc cccgtggcgg gttggtttgt tgaagcacac ggctggtcga cattctatct
    23581 attctccgtc gccgctgccg taccggggct tattttgctg ctggtttgcc gccagacgct
    23641 tgaatataca cgagtaaatg acaactttat ctcccgtacc gaatatccgg caggttatgc
    23701 ctttgccatg tggacactgg cggcgggcgt cagcctgttg gccgtgtggt tacttctgtt
    23761 gacgatggac gcgctggatt tgacgcactt ctctttcctg cctgctctgc tggaagtcgg
    23821 ggttttagtc gccctttctg gcgtcatgct tggtggtttg ctggattatc tggcgctacg
    23881 aaaaacgcat ctgacgtaat ctgtaaatat tatttagctg tagtaatcac tcgccgtaat
    23941 tattacggct aaataaacat cagcatcgct tattaatatt tgttcttttg tgcggcttag
    24001 cgtttggaaa ttattgacgc catttataaa ttctattttt cttacacgat tcagctaatg
    24061 agtctttatt ttctcaccac ccagttgtca ctttaatgat aattatttgt taaataattg
    24121 ttttatttcg cattggttat accaattgcc cgccaagact ccttctgcac tccccttttg
    24181 tta
//
LOCUS       DAEEGA010000060        22777 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEEGA010000060 DAEEGA010000000
VERSION     DAEEGA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22777
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.060"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="KLH46_002598"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="KLH46_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBE0379233.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="KLH46_002599"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="KLH46_002599"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBE0379234.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="KLH46_002600"
     CDS             complement(3736..4092)
                     /locus_tag="KLH46_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBE0379235.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="KLH46_002601"
     CDS             complement(4096..4512)
                     /locus_tag="KLH46_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBE0379236.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="KLH46_002602"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="KLH46_002602"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBE0379237.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="KLH46_002603"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="KLH46_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="KLH46_002604"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="KLH46_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBE0379238.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="KLH46_002605"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="KLH46_002605"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBE0379239.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="KLH46_002606"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="KLH46_002606"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBE0379240.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="KLH46_002607"
     CDS             10521..11504
                     /locus_tag="KLH46_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBE0379241.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="KLH46_002608"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="KLH46_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBE0379242.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="KLH46_002609"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="KLH46_002609"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBE0379243.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="KLH46_002610"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="KLH46_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="KLH46_002611"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="KLH46_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBE0379244.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="KLH46_002612"
     CDS             complement(15306..15515)
                     /locus_tag="KLH46_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBE0379245.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="KLH46_002613"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="KLH46_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBE0379246.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="KLH46_002614"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="KLH46_002614"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBE0379247.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="KLH46_002615"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="KLH46_002615"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBE0379248.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="KLH46_002616"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="KLH46_002616"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBE0379249.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="KLH46_002617"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="KLH46_002617"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBE0379250.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="KLH46_002618"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="KLH46_002618"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBE0379251.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..>22777)
                     /locus_tag="KLH46_002619"
     CDS             complement(22469..>22777)
                     /locus_tag="KLH46_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379252.1"
                     /translation="TEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSSQRLHDMLN
                     PTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALFPIFTASWRATRIKNKGI
                     "
BASE COUNT         5854 a         5925 c         5486 g         5512 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gttgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaatccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcaaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa taatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gacaaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaacagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagt
//
LOCUS       DAEEGA010000061        22159 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEEGA010000061 DAEEGA010000000
VERSION     DAEEGA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22159
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.061"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..408)
                     /gene="sxy"
                     /locus_tag="KLH46_002620"
     CDS             complement(<1..408)
                     /gene="sxy"
                     /locus_tag="KLH46_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBE0379253.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
     gene            627..1136
                     /gene="sulA"
                     /locus_tag="KLH46_002621"
     CDS             627..1136
                     /gene="sulA"
                     /locus_tag="KLH46_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBE0379254.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            1492..2538
                     /gene="ompA"
                     /locus_tag="KLH46_002622"
     CDS             1492..2538
                     /gene="ompA"
                     /locus_tag="KLH46_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBE0379255.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(2614..3066)
                     /gene="matP"
                     /locus_tag="KLH46_002623"
     CDS             complement(2614..3066)
                     /gene="matP"
                     /locus_tag="KLH46_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBE0379256.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            3251..5011
                     /locus_tag="KLH46_002624"
     CDS             3251..5011
                     /locus_tag="KLH46_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBE0379257.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            5080..5598
                     /gene="fabA"
                     /locus_tag="KLH46_002625"
     CDS             5080..5598
                     /gene="fabA"
                     /locus_tag="KLH46_002625"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBE0379258.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="KLH46_002626"
     CDS             complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="KLH46_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBE0379259.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="KLH46_002627"
     CDS             complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="KLH46_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBE0379260.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="KLH46_002628"
     CDS             complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="KLH46_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBE0379261.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="KLH46_002629"
     CDS             complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="KLH46_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBE0379262.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(9679..11586)
                     /locus_tag="KLH46_002630"
     CDS             complement(9679..11586)
                     /locus_tag="KLH46_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0379263.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="KLH46_002631"
     CDS             complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="KLH46_002631"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBE0379264.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            13950..15059
                     /gene="ycbX"
                     /locus_tag="KLH46_002632"
     CDS             13950..15059
                     /gene="ycbX"
                     /locus_tag="KLH46_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBE0379265.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="KLH46_002633"
     CDS             complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="KLH46_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBE0379266.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="KLH46_002634"
     CDS             complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="KLH46_002634"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBE0379267.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(16893..17632)
                     /locus_tag="KLH46_002635"
                     /pseudo
     CDS             complement(16893..17632)
                     /locus_tag="KLH46_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(17598..18113)
                     /locus_tag="KLH46_002636"
     CDS             complement(17598..18113)
                     /locus_tag="KLH46_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE0379268.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(18121..18663)
                     /locus_tag="KLH46_002637"
     CDS             complement(18121..18663)
                     /locus_tag="KLH46_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0379269.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGYFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKFT
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(18761..19744)
                     /locus_tag="KLH46_002638"
     CDS             complement(18761..19744)
                     /locus_tag="KLH46_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0379270.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGLRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            complement(19735..22107)
                     /locus_tag="KLH46_002639"
                     /pseudo
     CDS             complement(19735..22107)
                     /locus_tag="KLH46_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
BASE COUNT         5495 a         5803 c         5203 g         5658 t
ORIGIN      
        1 cacctccccg agaatcgctt ccagatgaaa agacatattg ggcaaatctt tcagtctttc
       61 ccgggtattg cgcgtgcttt tctctttcag cgctgcatcg agagaatatt tcgacagacg
      121 caccagcttc agttgatttc gccatagact ttcatcaacc cgatagtaat tgagggtaac
      181 ggatcggcca cactttttat atgtcagcca gacaggcgga tgttttacac agtactgtgc
      241 actttgctca caagcccgaa gatacaactc accatcagaa accatcgcaa acaccgtgtc
      301 gtcaacggtc aggctgtaac tgccaaacaa tgatcggtat tcaattgtgc ccaacgttgc
      361 caagtattct tgtgatttat agatccgctt ataggagagg cttttcataa aattcctttt
      421 aaaatcataa cataaaagaa tgattcacat taacggatcc gttaactacg aaaataggca
      481 acttattctt aaggggcaag attaatttat gttttcccgt caccaacgac aaaatttgcg
      541 aggctctttc cgaaaatagg gttgatcttt gttgtcactg gatgtactgc acatccatac
      601 agtaactcac aggggctgga ttgattatgt acacttcagg ctatgcacat cgttcttcgt
      661 cgttctcatc cgcagcaagt aaaattgcgc gtgtctctac ggaaaacact acagccgggc
      721 ttatcagtga agttgtctat cgcgaagatc agcccatgat gacgcaactt ctactgttgc
      781 cattgttaca gcaactcggt cagcaatcgc gctggcaact ctggttaaca ccgcaacaaa
      841 aactgagtcg ggaatgggtt caggcatctg ggctaccctt aacgaaagta atgcagatta
      901 gccagctctc cccttgccac acagtggagt caatggttcg cgctttacgc acgggcaatt
      961 acagtgtggt gatcggttgg ttgacagatg atttgactga agaagagcat gctgaacttg
     1021 ttgatgcggc aaatgaaggt aacgctatgg ggtttattat gcgtccggta agcgcatcct
     1081 ctcacacaac gagacaactt tccgggctaa aaattcactc taatttgtat cattaagtaa
     1141 atttaggatt aatcctagaa ctttttttgt cgcccagcca atgctttcag tcgtgactaa
     1201 ttttccttgc gaaggcttgt ctgaagcggt ttccgcgttt tttttctgta aattgtcgct
     1261 gacaaaaaga ttaaacatac cttatacaag actttttttt catatgcctg acggagttca
     1321 cacttgtaag ttttcaacta cgttgtagac tttacatcgc caggggtgct cggcataagc
     1381 cgaagatatc ggtagagtta atattgagca gatcccccgg tgaaggattt aaccgtgtta
     1441 tctcgttgga gatattcatg gcgtattttg gatgataacg aggcgcaaaa aatgaaaaag
     1501 acagctatcg cgattgcagt ggcactggct ggtttcgcta ccgtagcgca ggccgctccg
     1561 aaagataaca cctggtacac tggtgctaaa ctgggctggt cccagtacca tgacactggt
     1621 tttattccta acaatggtcc gacccacgaa aaccaactgg gtgcaggtgc ttttggtggt
     1681 taccaggtta acccgtatgt tggttttgaa atgggttacg actggttagg tcgtatgccg
     1741 tacaaaggcg acaacatcaa cggcgcatac aaagctcagg gcgttcagct gaccgctaaa
     1801 ctgggttacc caatcactga cgatctggac atctacactc gtctgggtgg tatggtatgg
     1861 cgtgcagaca ccaaggctaa cgtacctggt ggcgcatcct ttaaagacca cgacactggc
     1921 gtttctccgg ttttcgcagg tggtgttgag tacgcgatca ctcctgaaat cgctacccgt
     1981 ctggaatacc agtggaccaa caacatcggt gacgcaaaca ccatcggtac tcgtccggac
     2041 aacggtctgc tgagcctggg tgtttcctac cgtttcggtc agggcgaagc agctccggta
     2101 gttgctccag ctccggcacc ggaagtacag accaagcact tcactctgaa gtctgacgtt
     2161 ctgttcaact tcaacaaagc aaccctgaaa ccggaaggtc aggctgctct ggatcagctg
     2221 tacagccagc tgagcaacct agatccgaaa gacggttccg tagttgttct gggttacact
     2281 gaccgcatcg gttctgacgc ttacaaccag ggtctgtccg agcgccgtgc tcagtctgtt
     2341 gttgattacc tgatctccaa aggtatcccg gcagacaaga tctccgcacg tggtatgggc
     2401 gaatccaacc cggttactgg caacacctgt gacaacgtga aacagcgtgc tgcactgatc
     2461 gactgcttgg ctccggatcg tcgcgtagag atcgaagtta aaggtatcaa agacgttgta
     2521 actcagccgc aggcttaagt tatcgtctgg tagaaaaacc ccgctgctgc ggggtttttt
     2581 ttgcctttag taaattgaac tgactttcgt cagttattcc ttacccagca atgcctgcag
     2641 atcctgctta agagaagaca ttttattcgc gtatttctct ttgttttccg catcttcaat
     2701 cagctgaaca atcgtttcag aaagcgtttt accgcgacgc tgtgcaagac cagccagacg
     2761 ttgccagaca ataaattcca gatcgatcga ttttttgcgc gtatgctggt gttccgcatt
     2821 aaaatggcgc ttccgtctgg cccgaatggt ctgcttcatg cgattgacca gttccggatt
     2881 catatgcttg tcaatccagc catttaccag cacgggttca ttttcgagcg agagcaacac
     2941 atcgacggct tcctgggcgg cactggcttc tatgtaacgg gtaattaact ccccttcgcg
     3001 atgcttcttc accagatact tccatttcca accgctttca agattttcaa gttgttgata
     3061 tttcatagcg atctcaatgt taccgtgtaa ctctttacag aatatcagct ttttaggcca
     3121 gtgaagaaaa gaatctccat cctgtgagct ttaacgccca gccaggagta ttgtcggtgc
     3181 tttctacgtg tgctgtagtg ccggttacgg tataatcgcg gctttgacaa cagactaaaa
     3241 aacatcaact ttgaccatta cgaaacttgc atggcgtgac ctggttcctg ataccgatag
     3301 ctatcaggaa atatttgctc agccacattt gattgacgaa aacgatcctt tattcagtga
     3361 tactcaaccg cgactgcaat ttgcgctgga gcagttgctg catacgcgag catcctcctc
     3421 ttttatgctg gctaaggccc cggaagagtc tgagtatctg aatcttattg ccgatgccgc
     3481 gcgtacgcta caaagcgatg caggccaact ggtgggcgat cactatgagg tttccggcca
     3541 ctccatccgc ttacgtcacg cagtgagtgc agatgataat tttgcgactt taacgcaagt
     3601 tgtcgctgcc gactgggtag aagcggagca actctttggc tgcctgcgcc agtttaatgg
     3661 cgacattacc ctgcagcctg gtctggtgca tcaggcaaat ggcggtattc tcatcatctc
     3721 tttgcgtaca ctgctggcgc aacctctgct gtggatgcgg ctgaaaaata tcgttaaccg
     3781 cgagcgtttt gactgggttg cgtttgatga gtcgcgccct ctccccgtct ctgtgccttc
     3841 gatgccattg aagctgaaag tcattctggt aggcgaacgc gaatcattgg ctgatttcca
     3901 ggagatggag ccagagcttt cagagcaggc tatttatagc gaatttgaag atactctgca
     3961 gattgtcgat gcggagtcag taagccagtg gtgtcgctgg gtgacattta ccgccagaca
     4021 taatcactta cctgccccgg gagcggatgc ttggccggta cttatccgcg aagcagcacg
     4081 ctacaccggt gaacaagaaa cacttccgct tagcccgcag tggatcctcc gccagtgtaa
     4141 agaggtcgcc tccctgtgtg atggcgacac cttctccggc gagcagctaa acttaatgct
     4201 gcagcagcgt gaatggcgcg aaggtttcct cgctgaacgt atgcaggatg agatccttca
     4261 ggagcaaatc ctgattgaaa ccgaaggcga acgcatcggg caaattaacg ccctttcggt
     4321 cattgaattt ccgggtcatc cacgcgcttt tggcgaacct tctcgcatta gctgcgttgt
     4381 gcatattggc gatggtgaat tcaccgacat cgaacgcaaa gcggagcttg gcggcaatat
     4441 ccatgcgaaa gggatgatga tcatgcaggc gttcctgatg tcggaactac agcttgagca
     4501 acagatcccc ttctcagcat cgctgacatt tgagcagtca tacagtgaag tggatggcga
     4561 tagtgcctcg atggctgaac tctgcgccct gataagcgcc ctcgctgatg tgccagtgaa
     4621 tcagagtatc gctatcacag gttcagtcga tcagttcggc cgcgtccagc cggtcggtgg
     4681 tttaaatgag aaaatcgaag gcttctttgc tatttgccag caacgtgagt taacggggaa
     4741 acaaggtgtc attatcccca ctgctaacgt tcgccattta agtcttcaca gtgaactggt
     4801 gaaagcggta gaagaaggca aattcaccat ctgggcagta gacgatgtga ctgacgcact
     4861 gccgttatta ttaaatctgg tgtgggatgg cgaaggccaa acgacgctga tgcaaaccat
     4921 ccaggaacgt atcgcgcaag catcgcaaca ggaaggacgt caccgttttc catggccatt
     4981 acgttggctg aactggttta ttccgaactg atcggacttg ttcagcgtac acgtgttagc
     5041 tatcctgcgt gcttcaataa aataaggctt acagagaaca tggtagataa acgcgaatcc
     5101 tatacaaaag aagaccttct tgcctctggt cacggtgaac tgtttggcgc taaaggcccg
     5161 caattgccag caccgaacat gctgatgata gaccgtgtgg tcaaaatgac cgaaacgggt
     5221 ggtaacttcg acaaagggta tgttgaagca gaactggata tcaatccgga tctgtggttc
     5281 ttcggatgcc acttcattgg cgatccggtt atgccgggat gcctgggcct ggacgcaatg
     5341 tggcagctgg tagggttcta cctcggctgg ctgggcggcg aaggtaaagg ccgcgcgctg
     5401 ggcgttggcg aagtgaaatt cactggtcag gtactgccga cagcgaaaaa agtgacctac
     5461 cgtattcact ttaaacgcat tgttaatcgt cgtctgatta tgggtctggc agatggcgaa
     5521 gtgctggttg attgtcgtct gatctatacc gccagcgacc tgaaagtcgg tctgttccag
     5581 gatacgtctg ccttctgatt atcatcagac agagtatcaa aaggcgaaac ctccgcaatg
     5641 cggaggtttc tttttaaaga gacagaatca ggccattact accctgtccg ccatggcttc
     5701 tcgccagcct cccagccatt gtgacctttg actcagcgtc tgatagggac acatttcttt
     5761 tgagcgtccg gcgatgccgg cctgataacc acgttgatgt gcccgttcca ggcgatctcg
     5821 tttttgtctc ttcatgcctc gtttccctca tactggtttc tggtggaaaa gaaaacagtg
     5881 attactaaat gtgcaatcac agttaagcaa taccgcgaat cataactgcc gtcaatgcgc
     5941 aaaattcacg ccactgtcat atttgtgagc tacacgggca tgtcggttgt acaaaaaatg
     6001 tgaagaggaa caaatttttc cgcacgttag aaacaaaaaa accgcctcag gcaacatacc
     6061 ggagacggtt atatttacaa atctttataa ttaaggtaga cgctttatct cttgtgcaat
     6121 agaagcggct tcctgactcc agacaccggc cagcacttta accatctcat cgtaaccatc
     6181 ctgagtttgc actccttcca gacggaacgg acgtttgatc agttgtccct ggtggttcaa
     6241 cagccactca ccactgacaa tgaccttgcc atcatagcga ccgttaaact ccgttacggt
     6301 aacattgagc gtgtcctggg cgcttcccag aggctgggag gcaaccaccc agccgggcag
     6361 ttgcgtactc aggttggcaa ccagggtgtt gcgcaactgt tgatccaacg ggctggccca
     6421 caagttgttg ttggcaatca catacttcac atcactggtt tgataaacca caccattccc
     6481 cgccagatag tcaggaacag tgacctgctc tacccataac agacgattgc cctggctggc
     6541 ggtactttgt gtaccgctct gcaccacagg taactggtaa tagtttttat taatttcgcc
     6601 ggagctgcat ccggccagcc acagtgctgc aatcgtcact agccactttt tcattgtttc
     6661 gccctcttcg gctctggatc ttttttgtcc ttcgcttcaa ataccagcgc gttactcttc
     6721 tcattgagcg ttttcagcac cggttgcagt tctcgcaaca cctgatcaag gcgctgcata
     6781 tccgccacca tcttgttgta ggctgcggag ccaggctgga agccctgcat gctgcgattc
     6841 aattcacgca acgttgactg catatccgtc ggcaactgct gcatcgactg gctggcgagg
     6901 atcttgttca tgctatccag cgtcgtttgc aggtttttca ttgtgcgctg actttcagaa
     6961 agcgtactgg ttgcctgttc aatcatcgga ttcaatggca gtttgttgat cttatccaac
     7021 gcttccatca gtcgttgctg gatttgcgcc aagccgccgc taacagtcgg gataatctga
     7081 taaccattaa attcacgaat accggttatt gcaggcgtat ttggatagaa atcgagatca
     7141 acatacagtg caccagtgac caggtttccg gttttcagcg atccgcgtaa accacgtttc
     7201 aacaattcgc caaggtgctc aacaacatcc gcattttcgc caagctgcat tttcagccgc
     7261 tctggctcga tacgaatcag taccggaata cggtaatcat cgttaaatgt ctgacgcata
     7321 ttcggcgcaa agaatggcac tttgcttacg gtacccaggc gaataccccg gaattctacc
     7381 ggagctcccg gttgcagacc gcgtaccgaa tctttaaaga acatcagata atcaatgtga
     7441 tcggtgtaca acgaatcctg aatgctcttc tgatcatcat acaaaacgaa agctgttttc
     7501 ggtgccactg gctgccctaa atccagacct tccggcacat caaagctaac accgccacta
     7561 agcagcgttg tcaatgagcc catctccaca cgcatccccg ctgacgtcag atcaaccgca
     7621 atgccactat ctttccagaa gcgaacattg ctggtcacca gtcggtcata aggtgcattg
     7681 atgaacagtt gataactgat attgcgtttt tgcgtatcga aggtgctggt ttcaaccgaa
     7741 cctacccgat agccacggaa cagcaccgga tctcctggcg agagctgccc ggcttttttg
     7801 ctatcgagaa tcacacggat acctttcgca tcaggcgggg ccaacggtgg tgagtccagc
     7861 aaatcgtatt tatccatttt gctgcctttc gcgcctggct gcagttcgat ataaactcca
     7921 gacagcagcg ttcccaggcc gctaatccct tcgcgaccaa tctgcggttt caccacccaa
     7981 aagacggtgt ctttatgcag caatttttcc ataccggaat tcagccgcgc tttgatttca
     8041 acgtgcgtca aatcatcagc cagtgtggcg ctttcaacca cgccgacgtc aacgctacgg
     8101 cttttaatgg tggttttgcc accttcaatt ccttccgcat tcgcggtgat cagggtcact
     8161 tccggtccct gatggctgta atgataaaaa agaacccagg ccccaatgag cgccgtgacg
     8221 ataggaaata tccacacggg agaccagttc ttcactttct ggattttggc ttccccatta
     8281 ttagattcca tactgtcagg actcctcatg ctctgattca ggttgacgat cccacgacaa
     8341 acgcgggtca aacgtcatag cagaaaacat tgtcattatg acgactaaag caaacattaa
     8401 tgcacccatt gccggataaa tactcattaa acctcccata cgcaccagcg ccgagagcac
     8461 cgcgataacg aaaacgtcaa tcatcgacca gcggcctaca aactcaacaa cttcataaat
     8521 caaatgcatt ctttcactgt cgcgcttgcc atgccctttg gcatcccaac acagccacgc
     8581 gatggcgatc atctttaacg ttggcaccat aatactggcc agaaagatca ccgcagcgac
     8641 gggataagag ccttcgctcc ataacagaat gaccccagcg agaatcgtcg acggcatctt
     8701 cgagcctaat aaatccgtca ccatgatggg caaaatatta gccggaagat acagcatgat
     8761 ggacgttaca agcagcgcga gtgtccactg caggctgttt ctacgccgaa cgtacccttt
     8821 ggtactacaa cgcgggcaca cgggttcatc agcaggaagg attgccgtac aacaggagca
     8881 cgaacgcagc ccctgacgta tccccgtgac gcctggtttt agcggctggc gcagttctgg
     8941 catcggggcg atgtcgtccc ataaccagcg acgatcaacg cactgaaaag cgcgcagttg
     9001 caggacacaa aataaacacc aggggagaaa gctgctgcct accccaatgc tgccgtaagc
     9061 catcagttta acgaaactga ccagcacacc cgcgaggaaa atctccgcca ttccccaggt
     9121 tttgagttga aaaagcaccc gtgccagttg ctcttttaaa cggaccggta attccgcgcg
     9181 attcaccagt aacagaatgg ttatcagaca aaacgcggga accagttgca caaacaacag
     9241 gaaaaaggtg ccgaggctgg cgtagtcctc agaaaaaagc acgccgggaa tttccagtaa
     9301 tgtaatttca ctggtaactc ctgcaacgtt catattcaca aaaggaaaca agttggacag
     9361 caacagcatg aacagtgcag ccaacgcata ggcggtggga cgctgccgag gggcatccca
     9421 cgccacggtt aacgttgtgc cacaccgggg acatgccgct ttctgaccat gctcaaggcg
     9481 cggtaacgcc accagcatgt cacactgcga gcacaggatg tgcttcgcgg catgatgatg
     9541 ttcgcacatt ggtactcctt aatgatgcgc tattttacag atgactaaca aagtgttggt
     9601 tttcatgcct gataaaccca gttcatcagg caattttacc gttgtcagct gtctctgttt
     9661 aaatcgacta ttttgcgatc agccaccatt ttttaacgct tcaagatact cccagcgttc
     9721 aaaggcttgc tccagctcct gctctgcagc agccatatca gcaagcactt tttgcgtctg
     9781 ctcatgcggc tgactgaaga aggaagcatc cgccacttgc gtctgtaggg cttccagctt
     9841 cgcctccaga tcttcgagca attgcggtag ctgctccagt tcgcgctgca atttatagct
     9901 tagtttgcta ctgctgcgtt ttacagtttc tgcttttggc gcggcggctt cttcgctttt
     9961 tttcaccgca ggctgtttga gtgccacata ttgttcttgc tgaccacggg catcatgata
    10021 accgccaaca taacgaccaa ttttaccgcc gccttcgaag atccagcatt ctgtaacggt
    10081 gttatcgaca aactgacgat cgtggctaac cagcaatacc gtgccctgat agctgtcgat
    10141 cagttcttcc agcagttcca gcgtttcgac atcaagatcg ttggtcggtt cgtcaagaat
    10201 caataagttg cttggtttga ggaacaaacg cgccagcagc aagcggttcc gctcaccgcc
    10261 agaaaacgca cgtactggcg tcatcgcccg tttcggatgg aacagaaagt cctgcaaata
    10321 gcccaatacg tggcgtggct tgccattaac catcacctct tgcttacctt cggcaaggtt
    10381 atccatcacc gttttatcgg gatccagttc cgcgcggtgc tgatcgaaat aagccacttc
    10441 cagtttggtg ccaacgtgaa tacgcccgct gtccgcttga agctgaccga gcatcagttt
    10501 cagcagcgtg gttttgccgc acccattcgg accaatcagg gcaattttgt cgccacgtag
    10561 aacctgggca gaaaaatctt tcaccagttg cttaccgtta acctggtagc aaacgtcttc
    10621 catttcgaaa acgatcttac cggagcggct ggcctcttcc acctgcatct ttgcggtacc
    10681 catcacttcg cgacgttcac cacgttcgcg acgcatcgct ttcagggcgc gtacgcggcc
    10741 ttcattacgg gtacggcgtg ctttgatccc ctggcgaatc cacacttctt cctgcgccag
    10801 tttgagatcg aactcggcat tttgtagttc ttccacgcgc agggcttctt ctttttcctg
    10861 caggtactgg tcgtaattcc ctggataggt caccagcttg ccgcgatcga gatcaacaat
    10921 gcgcgtcgcc atattgcgga taaacgaacg gtcgtgggag atgaaaataa tcgtcccatt
    10981 gaaggtttta aggaacccct ccagccagtc gatggtttcg atatccagat ggttagtcgg
    11041 ttcgtccagc agcaatacgc gcggattgct caccagcgcg cgtcctaatg ccgctttacg
    11101 caaccagccg ccggaaagcg acgacagcgc aacgttagga tctaacccca gttgcgccag
    11161 cacttcgttg atgcggtttt ccagctgcca caggttgtgg tgatccagct gttcctgaac
    11221 cttcgccagt tcgttgagat ttttctcgct cgggtcgttc atcaccaggc gcgaaatatc
    11281 gtgatagcgt ttcagatatt ccgcttgttc ttcaatgcct tcggcaacga aatcataaac
    11341 gctaccctca acgttacgcg gtggatcctg ttgcagacga gctacaatca aatcttgctc
    11401 ataaataatg cgaccgtcat ccagcccttg ttcacggttg aggattttca ttaacgtcga
    11461 ttttcctgcg ccgttgcggc ccaccagaca aacacgttcg ttatcttcga tatgcagttc
    11521 tgcgttatcg agaagcggcg cgtcgctgaa cgacagccat gcgccatgca tactgattaa
    11581 tgacattact attcctttca ggctgcggta atcagccagc agttgtggat ctggcggtta
    11641 cgggcgaaat cctgggagag cgttttttgc gtaatttctt gtgctttcag tcccagtttt
    11701 gccaggccgt cgagatccat acggaagcca cgtttgttgt tcgagaacat gatcgtccca
    11761 cctgcacgca gcagacgttt caaatctttc atcagcgcca gatgatcgcg ctgaacatca
    11821 aacgcatctt ccattcgttt tgagttagag aaggttggcg gatcgataaa gatcagatcg
    11881 aactgttcat ttgcctcacg cagccacgcc aggcaatcgg cctgaatcag gcgatgcgca
    11941 cgcctagtca ggccattcag acgcaggttg cgttctgccc attccagata agtacgcgac
    12001 atatccacgg tggtggtgct gcgtgcaccg cctaatcccg catgcacggt ggcgctgccg
    12061 gtataagaaa acaggttgag gaaatctttg cctttgctca tctgaccgag catacggcgg
    12121 gcgatacggt gatcgaggaa cagaccagta tcgagataat ccgtcaggtt cacccacaag
    12181 tgagcgttat attcggtaac ttcaagaaac tcgcccttct cgcccagttt ctggtactgg
    12241 tttttgccct tatggcgttc acgggttttc agcaccagtt tgtttggcgc aatccccagt
    12301 accgaaatgg ttgcagcgat aatatcgaac agacgctgac gcgctttgtg cgcatcaata
    12361 gtttttggcg gcgcatactc ctgcaccacc acccagtcgg cataacggtc aacggcaacg
    12421 ttatattccg gcagatcggc gtcatacagg cggtaacatt caatcccttc ctggcgtgcc
    12481 cacttctcga attttttgag gttcttacgc aggcggttgg cgtaatcttc cgctaccatt
    12541 gccggtttac tgtctggtgt gctttcggca acgtggtaat ttttctgtac gcagtccagc
    12601 gggccgtttt tcgccttgta ctgtttgtct gcacgcagct gcaggcagct tagcagatcc
    12661 ggcgaggcac taaacaaaga gagattccag ccaccgaact ggtttttcat gattcggccc
    12721 agcagactat gcagcgcaat cagcgccggt tcgctgtcca gacgttcacc gtatggcggg
    12781 ttgctcaaca ctgtaccgta cggccctttc ggcagcggat tggtcagttg cgcgacatct
    12841 ttcacctcaa aggtgatcag ttcaccaatc cccgcaagac gggcgttagt gcgcgcacgt
    12901 tgaatcaccc gtgcgtcgct gtccgaaccg taaaaatgag agctatactc agccaggcct
    12961 ttacgggcgc gagtttgcgc ttccgctttc acttcctgcc agatagcttc atcatgctgc
    13021 gcccagccgc taaagcccca acgcccacgg tgcaagcctg gtgcgcgatc ggtcgccagc
    13081 atcgccgctt caatcagcaa cgtaccggaa ccacacatcg gatcgagcag cggtgttcct
    13141 gactgccagc cggatcgcat cacaatagcg gctgccaggg tttctttgat cggcgcaata
    13201 ccagcacgat cgcgatagcc acgcagatgt aaaccatcac cactcagatc aagagcgata
    13261 ctggcggttt ctttatgcag ccagacgtta acgcggatat ccggcgcatc gcgatcaaca
    13321 tttggacgcg gcagattttt ccgcgtgaaa gcatcgacga tcgcgtcttt cactttcatc
    13381 gcgccgtact gactgttgcg gatggtgtca ttcaaaccac tgaagtggac agcgaaggtc
    13441 gcgccaggat taaacatctc tgtccagttg atcgcctgaa caccgagata gaggtctaaa
    13501 tcgctgtaaa ccttacactc gcccagcggc aacataatac gcgaggccag gcggctccac
    13561 atcaggctct ggtaaacaag ccgtgtgtcg cccttgaaat ggaccccacc ctgaaccacc
    13621 tggcattcaa cggcccccag attttccagt tcagttttta acagctcttc cagcccacgg
    13681 gccgtactgg caaacagaga attcatatcg tcacttttac tctaagagcc taccgcagta
    13741 ggcgtaattg ttgcagtcag tctggacatg gagagcgcgg agaaaccgga gcgtacacaa
    13801 gtacgtgagg atttcgagca caacccaggg ccaaactgac aaataaaata gcctaacggg
    13861 acaggcgcta agaaaattgt tgcgcattat agctaataag cgcgtcatgt cataaagttg
    13921 agggcttatt ttcatttgag gaccgcaccg tggcgacatt aacccggctt tttattcatc
    13981 ctgttaaatc gatgcgcggc attggtctta cacatgctct ggctgatgtc agtggtctgg
    14041 ccttcgatcg catctttatg atcacggaac ctgacggtac gtttattacc gctcgccagt
    14101 ttccccagat ggtacggttt actccttcac ctgtgcatga tggcttgcat ctcaccgcac
    14161 cagatggcag tagcgcatat gttcgttttg ctgatttcgc cacacaagac gcaccaaccg
    14221 aagtttgggg cacacatttt accgcgcgaa ttgcgccaga cgcgatcaac aaatggctaa
    14281 gtggattttt ctcccgcgaa gtgcaattac gctgggtggg gccacaaatg acccggcgcg
    14341 tgaaacgcca caacactgta cctctgtcat ttgctgatgg ctatccttac cttcttgcta
    14401 acgaagcctc gttacgtgat ctccaacaac gttgtccggc cagtgtaaaa atggagcaat
    14461 tccgcaccaa tctggtggtt tccggcgcgt cagcttggga agaagatagc tggaaagtga
    14521 ttcgcattgg tgatgtggtg tttgatgtgg ttaaaccttg tagccgctgt attttcacca
    14581 ccgtcagccc agaaaaaggg caaaaacatc cggcaggcga accattaaaa acattgcaat
    14641 ctttccgcac tgcccaggat aacggcgatg tcgattttgg tcagaattta attgcccgta
    14701 atagcggcgt gattcgcgtt ggcgatgagg tggaaattct ggcaacggct ccggcaaaaa
    14761 tttacggcgc agctgccgct gatgataccg ccaacatcac gcaacaaccg gacgcaaatg
    14821 tagatattga ctggcaggga caggcatttc gtggaaataa ccaacaagtg ttgctggagc
    14881 aattagaaaa tcagggaatt cgtatccctt attcttgccg cgcgggcatt tgtggaagtt
    14941 gccgtgttca gcttttagaa ggcgaagtca cgccgctgaa aaaatcagca atgggcgatg
    15001 atggcaccat tctttgctgt agctgtgtac cgaagactgc ccttaagctg acgcgttaga
    15061 ctgcctgttc gaggctgaag ctgtcaacat tcacctgcgg tttcagcctg tcgttcatga
    15121 ttttaatggc gtcgcccaac tgcatcgcgc gacccgcaat aacaacgcag ggttgtgcca
    15181 gcaggcatag cgcggcattt tcgccagatt caacgaccag caaattgacc tgctcgtgag
    15241 tatcgctcaa ccaaacgcag gccgcatcgc ccggcatggg cacccacatt tcaccatggg
    15301 agacaaaatg ccagcttttc ggcatttgcg gtttgagata acgaattgct accagtgcat
    15361 tcagcaccag ttcggctttt tggtctttag ataaattaaa atcgcggcac ttttcttcaa
    15421 aagagaaata gagcgcggca tcatcaacac aaaagcctgc gggagaaaag gcatctggag
    15481 tcaacatttt gcgcgcaaaa cgtgagcgaa atagcatacc attggctaaa tcgagcatca
    15541 ttcgatcgtg ctcttcatcg taataccaac gccagttatc gtctggttta attcgcatct
    15601 gcctctcccc taccctataa aaccgcagca acacaatcca ttgtcgcttc gcttaataac
    15661 ctcaacaata aaagtacaaa atgtctaaat aagcaacaac gatgaatata aaacaaccag
    15721 ggctgaaaat aaagccctga ttatcttata aagaggaatt cgaagaaata attagatatg
    15781 ggtaacgatt tctttaatca gcggcggacc tttaaaaata aaaccagaat aaatttgcac
    15841 cagtgaggca cccgcagcaa tcttttcacg cgcagcgata accgagtcga tgccgccaac
    15901 accgatgatc ggtaagcgac cgtttaattc tagtgacaag cggcgaataa tttctgtgct
    15961 ttttaactga agcggacgac cacttaagcc accggtttga tcgcaatttt tcattccctg
    16021 aaccagagaa cgatcgagag tggtattggt tgcaataacg ccatcaatat tatggcgaac
    16081 taaactatcg gcaacctgga tcaattcgtc ttcagaaaga tccggcgcga tcttcactgc
    16141 gatcggcaca tatttatggt gcatcgcttg caaatcattt tgcttatttt taatcgcggt
    16201 taagagatca tccagcgctt caccatattg cagcgtgcgt aatcctgggg tattcggcga
    16261 tgaaatattg atggcgatat atcccgcata ggcatagatt ttttccatac aaatcagata
    16321 gtcatctttg ccctgctcca ctggcgtatc tttatttttg ccgatattaa tacccaagac
    16381 gccgtcataa tgggcctttt ttacgttctc tacgaggtta tcaacgccaa gattattaaa
    16441 gcccatacgg ttgatcaaac cttcggcatc taccagacga aagagacgcg gcttgtcatt
    16501 accaggctgt ggacgtggcg tgacggtacc gatctcgatc gatccaaatc ccatcgcgcc
    16561 taacgcatca atgcactccc cgtctttatc aagaccggct gccagaccaa gcggattttt
    16621 aaacgtcagg cccatgcagt taacaggttt cgcaggcact ttctgccgca ccagtgcttc
    16681 aaacggtgtt ccggtaatgc ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg
    16741 atcgagctgg aaaagggctt tacgaacgaa ggggtagtac atgaactctc ctggattccc
    16801 ggtgtgcaaa ccgggggcgt attatgtgcg agttcggacc aaaagggaat tgacctgcgg
    16861 caaaaaaaaa gcaaacgttt tcagaacctg tttcagggca gattttgctc cttctttgca
    16921 ctcaacccgc cgtaatcatt tatcgtccgc caggcgacat gacacggtgc tattacctga
    16981 caccagtttg tctgacgctg gcttttaggc ggcacgactc ccgcattact taacgcttta
    17041 ccatttacgc tcacctgaat caggttaatg tagtaaggcg ttgggttagt gagttgtaca
    17101 ccctgcgaat tccgtgtcca gcgtaattgt tgataagctt ccagcgggtc gcccggcaaa
    17161 ccttcgggtc gccagaacag tttaaatacc gagcgcatcg ccacttttac gctctgattt
    17221 tcaactttgc cggatggcac gctggcaatg cttaactcaa atagcgtttc gcgatccaca
    17281 ggcaagatgt cgctttccgt tctcagcaag cgcaacgtgc ctgtagtacc gggcttcagg
    17341 agaataagtg gcggagcggc cagtaatagt gctggcactg tcgacgcttc ccccccccgg
    17401 cccaacgcgt tgggcgattg attttgctgt taataagcca agattcctga ctggtattag
    17461 tcagtaaaat agatatcgat tctctgtctg ccggaaagat aaatcgcgtt ccgccgacca
    17521 ccactcctgc ctgcaattca cccgccacca gcagcgataa aacggcgaaa atcaagagcg
    17581 ctaatctgtt ccacgtatta gtcataatga agtacaaatg tcgcgctggc attcacttta
    17641 cccgactgca catcgctatt gacaggtcgc aattgcgcat aaaaagttaa tggcacctta
    17701 ccgctatgta ggctgtagtc tttactcgcc tggttaagcg gaatcaatat cttcgcatca
    17761 tccagaatcg caattgccag cccactggct gcatccgtac cactttcaat ggacagcatt
    17821 tcgctctgca ctccgtcggg ggttcctgaa aaggtgacat caaccgtgct gacatctgtt
    17881 ccgcaatcct gcaaattaat gatgaaagat ttctccgggc cataattccc atgaaaaaaa
    17941 cgtgtcagcg agacatcgcc taactgcaca ttgatttgct tgctatcctg tgccagcacg
    18001 catgtattac ggcgaaacga tccgctaatg ttgaccgtgc catcatgggc aaatagctca
    18061 cagccccccg ttaatcccag taagaacaga atccagatta tccgtttcaa catcactcct
    18121 tcactggtaa accagatcaa aatagagaac cgccgtcgca ttaccgcccg ttgctcccgc
    18181 ctttgtgaac ttataacgaa gttgatattt gagtgtgtta tccccggctt ttaagggcgt
    18241 aagaggctgg acctgattga gcgggatttc ttgctgactt tgcgcatcaa gaatttgcac
    18301 ggcaatcccc gttgccatac caccgcttcc gcctgtgtca gttagtttga gcagtgtcgg
    18361 cgcaagggtg tcagcctcgc cgctaaaaag gacgtccgcg ccagtaatac cagcggcaca
    18421 accggtgata tcaatattca aatcagcagc agtgcttacg gtattcgcgg cctgaaaatc
    18481 actgatatta aaataaccaa tatgaatatt ttgtaatgca cttttggtgg cgacatcaca
    18541 gccctgatcg accacattcc cataaatatg catactgact tgttcaggcc cagccatccc
    18601 gacatggatg ttaagaaggc taaagaacaa aataacgcac tgatatatcg tttttttctt
    18661 catacgttca cgcgttaatc aaaatttatt ctcaccgttg ccgtggcttc aaatcgcccg
    18721 gtttctggtg gtacgcctgt cgtgctgact ggatagacat tcattcgcgg atactgtctg
    18781 catcatcagg aatagagagc ggtagtgtcc caccagcagg ggaaataatg ttgttctggc
    18841 tatcggtcac caccacgccc acgccaggac gtgacgtctt aatcgcctgg ggatagctgg
    18901 ggtctgaggt tgccgtcaga ctcagattga cgttaactaa ccctgcgttt cccgtgttac
    18961 acctgaccgg aattgcaagt tctgccgttc tggccccaag aggcttatgc cctacaacac
    19021 gaaagttagc cgcaaatacg tcgcccagat caacctcgat tgtttctccg gcattgacgg
    19081 aacatgagcc aagtgaattg atcgtaccag acaggtaata cactaaaacc ggtgtaccag
    19141 tagtcgtgca ggaatcacct gcgggtatgt tgtagcattc aaataatcgc gccagcgcca
    19201 taggaggaat gacgaccgaa ccaacgaacg gctgcaatat tttcagggaa aagctaccgc
    19261 tattaccgct agccccctgg ccacctaaac gtagaccgcc gggatcgcaa atatgccatc
    19321 gtgtatctgc ccctgtgccc acgttttcga aaggaaccgc agtaggagaa ctgcctttaa
    19381 cctgcaataa aactttggcg gcaagatagt cattaatttt gtaccagttg tcgcctaaat
    19441 agactaacgg tgaatccgcg gcgaatgccc atcggccact tctgacattg tctttatcgc
    19501 aatcacagga aacataataa tcattcgatt cgttccactg ataaaatttg tctttaacta
    19561 ctccagccgt attttcttct tcatccagag ttttaacgcc aaaattgacg ccaatatacg
    19621 gattcccctg agtggtatga cactgcccga tataagcagc agtttgagcg caccatagca
    19681 ttaagacggc ggcaaaaaat agtcgtagta ataacacgca tttttctcca aagattatct
    19741 gcatatggca tccattcgcg taatcgcaga gttaatttgt tgttcggtaa gctggtaact
    19801 gatatgacaa atttgatcgg gagcttcgcc ccagcgtaca gttatcgctc cctgagcggg
    19861 caaaccggac atatagagcg agccatcatc atcaataatg ccgctattag tctgtatgcc
    19921 gtccagcgtc gctatcgcgc caaaacgcag cggtataccc tgctttgatg ttttcattaa
    19981 tacccttgct cccgcatggg cattgaactc cgccagaacc aacgcccctt tggtgggaac
    20041 cacgttgact actgcatttt caagatccac gttacgtttt aacgatgccg catcaagtgc
    20101 gattcgattt tcccgataag aagtggcata ggggacaacg gtatatccgc gccagtcagt
    20161 tttcagcccg gtgctgttcg ccacacgagt attctctgcg cctggtgctg caatcagcac
    20221 gttagtttcc cccagcgatt gccccagggt aatgccctgt gaatgggcaa ctaaactgcc
    20281 tgaaagagcg tagttcagtt gttgttgact gccgttatcg ctgtagttgt agcccactcg
    20341 ggcatctgca aacgcccctt tgtagtccat gctggcgcta ccattagccg ttttcccctc
    20401 gctgttatat ccctgttgca cgctgtagct caggttaccg tcatcgagta gtgtctccgt
    20461 taaacccagg ttttgtgtaa tgtgcccttc gttatcgata ctggtgttag aggtcatcca
    20521 ggcgtaattt tttttcctgg tatagctatc attacttctg gggagcaaca gattgagcgg
    20581 cagggaaaaa tttagtgcaa acacctgatc agcatcaggt tgaccacggg acttactgta
    20641 gttccaggaa acccccagcg agagatcttt aatttgcgtg ttgtagccaa actgtaatag
    20701 ccgatcctgt tgatcggtat gccagtaagt ttgctggcta ccacttaaat aaaacaaacc
    20761 gtactcgcct aattgctggg agatattgac ctgcagtttg ccacgtttgg tgtaaaacaa
    20821 attgtaataa cgcgaccaca tcggcgtatc ttcatcatca ccgtcattaa attcgtagcc
    20881 atccatatgt ttatacatgg tgtcggaaag ggtgtagaaa cccgacgtcg aatagcgata
    20941 gcccatcaat tgaaaattag tgcccaattg gttgagcgtt ttggcataca gaaaacgata
    21001 tgactgacct ttatgttcgc tttggtctgc cagcgtactc ttcgcctggg tcgcatcgaa
    21061 cgaaattgct ccgaaatcgc caaggttaaa tcccagacca aacatggcgg cacggtaata
    21121 ttcagcatat tgtccaccac cataccatgt cgttccccac ggtccgcccc actgcaacgt
    21181 ggtctgggca aatttgctct cctgctgttc attactattg gttctgtatt tcgccagtgt
    21241 caccgcgtat ttgattcgcc cctgacgctg gagtaatggc acgctggaaa acggtacgct
    21301 gtagctattg acgctgccgt ccgcttcttt gatttcaact aacaaatcac cgctcgacga
    21361 cgtggaatag agatcactaa tttcaaaagc accaggcgat acataagttt ggtaaatggt
    21421 gtaaccattt tgcttaatcg ttatctgggc attgcttttg gcgataccac gcaccacagg
    21481 cgcaaaccct ttcaggctgt ctggcagcat gttgtcatct gacgccagct gcacaccacg
    21541 aaagctgaca ctatcgaaaa aatcgcctgc agtataatca tcacctacgg tcagttcgcc
    21601 ttttagtgga ataaccgccc gctgcaaata gctgctgaga ttcttccatt ctgcggtttg
    21661 gccactactg cgactccatg ttgaattgtt gcgcaatctc catgggccta aattaacgcc
    21721 actgtttaaa ttcagaaaat agctgtcgcc agaatcactg tctgcgctgc tatgaatact
    21781 gttagccccg ctaaatagcg cgttgatccc ttcatcccaa cgttcagggg ggacataacc
    21841 acgggcatta cttttgatgg cgatttgcgg tactgaaatt tccagacgga gtttattcac
    21901 attaaacgtt acgctggcat ccggaataat ctccgccaat ggcacacaca cttgtttgtc
    21961 cagttgttta aaagccggaa acgcatcagt attaacaccc attgcatcca ggctttcagt
    22021 cgtcaaacag gcggcaatcc ccttctctgg cgttatctca acaaaattga cgttgcgcgt
    22081 atctacaatt gtctgattca gaacaatggt aatgactcca acttactgat agtgttttat
    22141 gttcagataa tgcccgatg
//
LOCUS       DAEEGA010000062        21988 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEEGA010000062 DAEEGA010000000
VERSION     DAEEGA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.062"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            61..1062
                     /locus_tag="KLH46_002640"
     CDS             61..1062
                     /locus_tag="KLH46_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBE0379271.1"
                     /translation="MHKSNCFDTARLVAAMMVLISHHYALSGQLEPYIFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSVLTYFLFGWMLNNFSTE
                     YFSHDIIRKTISSILMSQAPDADITSHLIHPGINGSLWTLPLEFMCYIITGVAVALLK
                     SGKTFIVILLIFVSLSLVESVSENKDVIFSIPVWLYPLRGMAFFFGATMAMYEKSWNV
                     SNVKISVISLLAMYAYASYGKGIDYTMACYILVSFSTIAICTSFGDPLVKGRFDYSYG
                     VYIYAFPVQQVIINTLHIGFYPSMLLSAIVVLFLAHLSWNLIEKRFLTRSSPKLSLN"
     gene            complement(1353..1787)
                     /locus_tag="KLH46_002641"
     CDS             complement(1353..1787)
                     /locus_tag="KLH46_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBE0379272.1"
                     /translation="MRITLQWDINEMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINSQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1759..2550)
                     /locus_tag="KLH46_002642"
     CDS             complement(1759..2550)
                     /locus_tag="KLH46_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0379273.1"
                     /translation="MAINNFKPFALDPNANVTSQADWEALPALLSGFTAGKASSAQVN
                     KAIRQASFIAAALAQYTANKSRLDVLDDGDLNGFISKMGTAFGKDFQALDATLSALAG
                     LATGANKLPYFTGNDTAAQTDLTSVGRDIIGKNTIADILTYLGLGEAAKRNVGTGANQ
                     IPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNVSSTYGMRFPMTFSRVLAVTVT
                     LNVTGASGQPTVSATNVHNTGFDITVSPGSGYGSSADAYYIAMGY"
     gene            complement(2550..3230)
                     /locus_tag="KLH46_002643"
     CDS             complement(2550..3230)
                     /locus_tag="KLH46_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HBE0379274.1"
                     /translation="MSKYTELITNYHATKPLFFDHIDLSTRPLIDVSSTMSGLITAFD
                     IDTAVGVQLDILGLWIGRSRIVSQPISGVYFSWDTDGLGYDQGIWKGPYDPDSGYTTL
                     SDETYRIILKAKIAINNWDGRNDSLPPILDAATEGSGLRMQIVDNQDMTISVWVFPET
                     DISDVSLELIAAIKQGYLTVKAAGVWAGDVETPSVEAPSEGSKFFGFDMDNEYIGGFD
                     VGAWGTIL"
     gene            complement(3227..4426)
                     /locus_tag="KLH46_002644"
     CDS             complement(3227..4426)
                     /locus_tag="KLH46_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBE0379275.1"
                     /translation="MALNLDTLGLSATVTAQGISAPDYQTILDTLTSYFRQIYGSDAY
                     LEPDSKDGQMVALVALAVHDANNTAIGIYNSFSPTTAQAAALSSNVKINGITRKVATN
                     STADLLLTGTAGTTITNGSARDKNGIIWNFPASVAIGVDGTVLVTATCANSGSVAALA
                     GTITTINTPTRGWVSVTNPVAATVGSPAETDAELRIRQGQSVALPSITPFEGVDGAIA
                     NVAGVTRHKLYENDTGATDSNGLPPHSISAIVDGGDVTEIAQTIRGSKGQGTATYGKT
                     SVTVPDTYGNPHVISFSRSTDVPIFVAITLKVFTGYTSQIGEQIKQAVADYINGLTIG
                     DDVLLSRIYSPANLGVVSGGNARYYDITDLLIGKSSGSVSASNIDIAYDASASCSTAN
                     ISITVTS"
     gene            complement(4427..4780)
                     /locus_tag="KLH46_002645"
     CDS             complement(4427..4780)
                     /locus_tag="KLH46_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379276.1"
                     /translation="MRYRREDTEGDYTFGQGDDTFLIDSPECVAQAVKTRFELWRGQW
                     FLDLTEGTPYVQSVLGKQRSDVYILAIRERIQDTPGVLSILSFDTNYDGTSRRVTFTS
                     SIDTIYGQTTVTSEA"
     gene            complement(4780..5520)
                     /locus_tag="KLH46_002646"
     CDS             complement(4780..5520)
                     /locus_tag="KLH46_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HBE0379277.1"
                     /translation="MPIPTQSQIGGEQQTAQAIADSVSTQMRVAMPGIIQSFDPETVT
                     CTVEVALRGIVGDGSTELKPLVDVPVIFPRGGGCTLTFPVKEGDECLLIFADRCIDFW
                     WQSGGVQETVDPRQHDLSDAFAIVGPQSQARKISGISTSAAQLRTDDGAAFVEVAAGH
                     NITIKTPGQLTATAEGGTTITSPTITLNGNVTINGNLSQGMGESGGTATMLGPVMVTN
                     DVTASGISVATHKHGGVQTGGGTTGGPQ"
     gene            5743..6033
                     /locus_tag="KLH46_002647"
     CDS             5743..6033
                     /locus_tag="KLH46_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBE0379278.1"
                     /translation="MGFRFRKRIRIAPGLAINISKSGVSTSIGGKGSTINIGKKGVKM
                     TNGLPGTGLSHTTNLYSPGKSTEKKQLTHKQKIIRNILFVIIVFIIIKAQYF"
     gene            complement(6057..6401)
                     /locus_tag="KLH46_002648"
     CDS             complement(6057..6401)
                     /locus_tag="KLH46_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379279.1"
                     /translation="MRHLLCTVTVFAVLISAPAFADQQCGDFKIHWADDGLARINGAK
                     PEMQKITFLKNKGDYNNIKMDWRMATDQPGRWVGLEYINRNGKAILNAQWLQASMDSP
                     RQYATYDCRKVK"
     gene            complement(6414..7478)
                     /locus_tag="KLH46_002649"
     CDS             complement(6414..7478)
                     /locus_tag="KLH46_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379280.1"
                     /translation="MSLNWMRHFELQLLDQNGQGVSLSDFKVTFQIEWADTRWPRVAN
                     VKIYNLSTDTTNKILGQEFAKIRIIAGYDGIAPDVDASQVGVSREISPDQIGQVNGQN
                     YGLIFDGDIRFTVTGKDNITDSWVLIQAIGDHEAFLYATTITTLAAGYTVADLHRATM
                     QDFNAFGVTQGITGDFPDTVFPRGRAIYSSSRNVMDNIAAQCKATWQLVDGQVQMVPE
                     DKYIHEAIVLNANTGLIGMPQQTMGGGVNVRCLINPNIRINGLIQLDQASVYRATLGN
                     SEIAQSPGRITETEENGNRVLTGTTSQAASIATDGVYIVKAIDYTGDTRGQAWYMDLM
                     CFARGARDLYSPKAMQGTTN"
     gene            complement(7481..7786)
                     /locus_tag="KLH46_002650"
                     /pseudo
     CDS             complement(7481..7786)
                     /locus_tag="KLH46_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120161.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7788..8390)
                     /locus_tag="KLH46_002651"
     CDS             complement(7788..8390)
                     /locus_tag="KLH46_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379281.1"
                     /translation="MDILSAIFRQQSRRIGILIPSVVVSEKHSDALEITEHPVEKPTT
                     NSASGFIADHAYKRPSEVTMECGFAGGGSLLDFIDTSSIGLSAGLSPKETYQQLLDLQ
                     SSRVPFDVVTGKRVYSNMLVRAIEVTTDKTSENVLNCTLTLREVIMSQTQSVSVADKS
                     DMQDGVSTSAVQNSGMKSTTPPNESLLSQLGGSVTSAFGG"
     gene            complement(8390..10405)
                     /locus_tag="KLH46_002652"
     CDS             complement(8390..10405)
                     /locus_tag="KLH46_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBE0379282.1"
                     /translation="MSGNADTIKDFLVSLGFDIDQAGANKFEAVLKGVTANVLKVGAV
                     VKGAALSIVGFTTQIANGLDKIYWASQRTGASVQGIKALGYAASQTGASAESAMSSLE
                     GLAGFMRSNPGAEGFLNRLGVQTRDASGKMRDTAAIFTGVGQKLNNMPYYRAKQYAQM
                     LGIDENTLMAMRRGMGQLSSEYALTAKRIGFNAESAAKQSNIFMTSMRNLTMTLGQAK
                     DKIGSNLAGGLAGSIDNFRRQILDNWPKIEAVITKIIKGILWAGDAITRVLWRTGQAV
                     EGVIAWFKKLNPATQQLIALFSGLLVAWRLLNTAFMSSPLGMITTLIIALGLLLDDYQ
                     TWKEGGKSLIDWGKWKTEIDQAVKMIGDLKKTVTDLTKALAKLLGIDPKSWSLKWDFS
                     NFISQMGEFGKMLNMIADLLNAIKDGRWADAASIGKQMLNQGSENPSAMPMVTDSANG
                     TADWIKEHWGFDPRSVGRTVRGWFGDDEPEQLGQSVKRPQPTKAGSELLGWMQPMFTN
                     LEQLYRLPEGLLRSVAITESGGNQFAVSGAGAKGLFQFMDGTARDMGLRGNDVFDPEK
                     SAQAAAKYLSQLLRQNGGDLSKALASYNWGIGNVKRYGMGLMPQETRNYIPKVMSNMP
                     TSAPVIQQETNINIHGVFDPREAARLTVDRQKGVNSQLTQQLPAGPR"
     gene            complement(10583..11008)
                     /locus_tag="KLH46_002653"
     CDS             complement(10583..11008)
                     /locus_tag="KLH46_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379283.1"
                     /translation="MEFEIKGVKYRTAKLSVFEQLKVSRKLLPVLAGMVSDFRSVQEK
                     ISSKDTEGAMATILPKIANAVSDLSDGDVDAILFPCLSVVSREHMKGWVPVCQHGEMA
                     FDDIDLLTMLQLVARVVADSLGNFLQGLPTSETPTPLAE"
     gene            complement(11012..11452)
                     /locus_tag="KLH46_002654"
     CDS             complement(11012..11452)
                     /locus_tag="KLH46_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HBE0379284.1"
                     /translation="MATYSFLDVTASLTGPTGVIDLGQGSANSEEGITQTMGGNKNTM
                     TIGADGEVMHSLHADKSGTITVTLLKTSPVNKKLSLAYNAQSQSSATWGNNVIVIRNT
                     ASGDISTARSCAFQKQPDFNNAKEGGTVAWVFDCGKIDQLLGEF"
     gene            complement(11463..12608)
                     /locus_tag="KLH46_002655"
     CDS             complement(11463..12608)
                     /locus_tag="KLH46_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HBE0379285.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYANDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKIPQTDAGVTRLLSNVEQSMDQSVTNGLVAAGVWNGGPIGQLNSGDTLT
                     KGYYVYAQPLSEQAQADREARKAPLIQVACKLAGAVHYADVQINVVR"
     gene            complement(12612..13169)
                     /locus_tag="KLH46_002656"
     CDS             complement(12612..13169)
                     /locus_tag="KLH46_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379286.1"
                     /translation="MGELRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLFPELINQQWVRRYDMTV
                     RLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(13144..13533)
                     /locus_tag="KLH46_002657"
     CDS             complement(13144..13533)
                     /locus_tag="KLH46_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HBE0379287.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(13520..14074)
                     /locus_tag="KLH46_002658"
     CDS             complement(13520..14074)
                     /locus_tag="KLH46_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379288.1"
                     /translation="MSFKSGVTTRVDNAQAILDALRSLTKKDVLVGIPEEDSEREDVP
                     FGNAGIGYVNEYGSPAQNIPPRPHLIPGVKSVEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRVAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(14071..14478)
                     /locus_tag="KLH46_002659"
     CDS             complement(14071..14478)
                     /locus_tag="KLH46_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HBE0379289.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKDLFPYFAGLFVAHYMTLWAADSRAMLVGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            14477..14711
                     /locus_tag="KLH46_002660"
                     /pseudo
     CDS             14477..14711
                     /locus_tag="KLH46_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14708..15649)
                     /locus_tag="KLH46_002661"
     CDS             complement(14708..15649)
                     /locus_tag="KLH46_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HBE0379290.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLAGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVVELAAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWAISTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPIQYRGLYQIATYYGKLGAVEPVYKETISYVDGI"
     gene            complement(15661..16164)
                     /locus_tag="KLH46_002662"
     CDS             complement(15661..16164)
                     /locus_tag="KLH46_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379291.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DADGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVNL
                     GSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDANGN
                     AEISWKI"
     gene            complement(16169..17389)
                     /locus_tag="KLH46_002663"
     CDS             complement(16169..17389)
                     /locus_tag="KLH46_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBE0379292.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGDHSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKVPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTVFKREVLAS
                     ADKTLVRQVVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRSTTGDAQRPNMGMTSIAS
                     LNKQNAEFWSNRKG"
     gene            complement(17404..18141)
                     /locus_tag="KLH46_002664"
     CDS             complement(17404..18141)
                     /locus_tag="KLH46_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBE0379293.1"
                     /translation="MATNRQSRQAEIRYRTSLRQIARAVGDIVNGRYDGSNDSVTEIM
                     DALERYSEIITPWATKVAENFTADIARQNEKQWRQHSRNISAELRNMVDRAPVGQVMK
                     SIVAEQIKYIKSLPLEAADRVYDIQNKAIEAVVAGGRAEPFAKEIAASGDVSRSRANL
                     IARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDG
                     MTGHAGELPNCRCYKEIVFPNPHSYLA"
     gene            complement(18029..19495)
                     /locus_tag="KLH46_002665"
     CDS             complement(18029..19495)
                     /locus_tag="KLH46_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HBE0379294.1"
                     /translation="MARNKQATRRTAQATADGYENFVARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVLTRR
                     IKEMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAFEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPMRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVAVNTTNAISTALGDGLMTRKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     ELGDKPPEPPGGDPISNEPTADSAGGRGYRKWALRWFK"
     gene            complement(19495..21117)
                     /locus_tag="KLH46_002666"
     CDS             complement(19495..21117)
                     /locus_tag="KLH46_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HBE0379295.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWDEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDESAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNSF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMDYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMISYAPMNSALNIWELLGRQA"
     gene            complement(21120..21692)
                     /locus_tag="KLH46_002667"
     CDS             complement(21120..21692)
                     /locus_tag="KLH46_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBE0379296.1"
                     /translation="MFRRCDLKQLTPKQELFCREYLKDLNATQAAIRAGYSEKTARAT
                     GSENLSKPDIADRIAELKAERNEEVGIDAAYVLRRLTEIDQMDVLDILLANGELKQIK
                     DWPKVWRTTLSGMDVVEMASADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
     gene            complement(21676..>21988)
                     /locus_tag="KLH46_002668"
     CDS             complement(21676..>21988)
                     /locus_tag="KLH46_002668"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBE0379297.1"
                     /translation="KDLVVRHKCDNPSCVNPDHLIIGTVADNNRDRSRRGRNSYPDRS
                     GEKNGMAKLKECDVICIRREYVRGSVGNGLRSLAKRYGVSTTMIADIVNHRNWSNVSE
                     V"
BASE COUNT         5001 a         6003 c         5440 g         5544 t
ORIGIN      
        1 aatggactat gacataaaga tctgatattt tcctcacaaa aacttaggga ttttatttta
       61 atgcataaga gcaactgctt tgatacggca aggcttgtgg cagctatgat ggttctaatc
      121 agtcaccact acgcgctctc tggacaactc gagccgtata tattcggctt tgaaagtgct
      181 ggcggcattg ctgttatcat attcttttcc atatcaggat acctgatttc aaaatcagca
      241 atcagaagtg attcctttat tgatttcatg gctaaaagag caagaagaat attccctgct
      301 cttgtccctt gctctgtact gacgtatttt ttatttgggt ggatgcttaa caacttcagt
      361 acagaatatt ttagtcatga cattatccgg aagaccataa gctctatttt aatgagtcag
      421 gctccagatg ccgacattac atcacattta attcatcccg gtattaacgg cagcttgtgg
      481 acactaccac tggagtttat gtgttacata ataactggag tggctgttgc tctactaaag
      541 agcggaaaaa cgttcatagt tatactattg atttttgttt cactttcatt agttgaaagt
      601 gtatctgaga acaaggatgt aatattctcc attccagtat ggctgtatcc tttgcggggg
      661 atggctttct tttttggtgc cactatggcg atgtatgaga aatcttggaa tgtaagtaat
      721 gtgaaaattt ccgtgatttc tttgcttgct atgtacgcat acgcgagcta tggcaaaggc
      781 attgactata caatggcttg ctatattctg gtttctttct caacaatagc tatatgcaca
      841 tcctttggag atccactggt taagggtagg tttgattact cttatggtgt atacatttat
      901 gcattccccg ttcagcaggt tattataaat acattgcata ttggctttta cccttcgatg
      961 ttgttaagtg ccattgtcgt tcttttcttg gctcatcttt cttggaatct aattgaaaaa
     1021 agattcctta cacgaagctc gccaaaatta tccctgaatt gaataataaa ggggccatcg
     1081 agcccctgaa taaatcaaag ccagatataa cactgcctgg cagtggacac cgttctgatg
     1141 cctcgtcctg caaggacgag cgaaggtgga gttcttttcc acgcagctat ttagtacagt
     1201 actgagtcat attgtttatg tatgagttaa cttccgtaat atcaattaaa taaaataagt
     1261 tgaggccatt gtagaagtga gcattatatc caggttatct ggacctgttg aaaaatgcgt
     1321 attattccaa caattaccct agattaacaa gtctatttat ttggatgcat aggccactcg
     1381 ggttttacta cattaacacg cataagcagt acgcgatatt ttttccatgc cagtaacagt
     1441 ttcgtttcat catctgttgc catacctaaa tcaaccgcat cctgaagcgg cgctattaca
     1501 ttcgttgcct ctgctatcag tgcagatttc ttttcatcat ttattaaggc taattgttct
     1561 ggtgttggag aaggacggtc aacaatgaca gggtgaccgt tctcaccaca ggtaatcatt
     1621 tttgttacac tctgtgagtt aatcagtgtt ttccactctg attccgagat ttccactgca
     1681 tcatcaggaa tattggtgcc gtgaaattca gtcgaataaa atccgtttgt cgatgcagaa
     1741 taaaaatatg acatttcgtt aatatcccat tgcaatgtaa tacgcatcag cagatgaacc
     1801 atatcctgaa ccgggcgaca ctgtaatatc aaatccagtg ttatggacat ttgtcgccga
     1861 tacagtcggc tgccctgacg cgccagtaac gttcaatgta actgtgacag caaggactct
     1921 tgagaatgtc atcgggaacc tcatcccgta tgttgaagat acattcgggg agccattcaa
     1981 ctgaccgcac tgtaaaatta atccagacgg aaatttttgc gcagcagccg ttgcggtatt
     2041 acttgacgcg aacaggctca tatcaggtat ctgattcgcc cctgtaccta cattcctttt
     2101 agccgcttct cccaaaccaa ggtatgtgag aatgtcagca atagtatttt tcccaataat
     2161 gtcacggcca acagaagtta aatcagtctg cgctgctgta tcatttccag tgaaatatgg
     2221 gagtttattt gcacctgttg cgagcccagc taatgccgac agcgtggcat caagcgcctg
     2281 gaaatctttc ccgaaagcgg tccccatttt ggatataaac ccgttcaggt ctccatcatc
     2341 aagcacatcc agcctgcttt tgttggcggt gtactgcgcc aacgctgccg cgataaagct
     2401 ggcttgccga atagctttgt tgacctgtgc gctggatgct ttacctgccg taaaccctga
     2461 aagcagagcc ggaagtgctt cccagtcagc ttgtgaggtg acgttagcgt tcggatcaag
     2521 cgcgaaaggt ttaaagttgt ttattgccat tagagtattg tcccccatgc tccaacatcg
     2581 aacccgccga tgtattcgtt atccatatca aacccaaaga atttagagcc ctcggatggt
     2641 gcttctaccg aaggcgtttc aacatcaccg gcccatacgc cagctgcttt aacggtgaga
     2701 tagccctgtt tgatagcggc aatcagttca agagacacat cagaaatatc agtctcgggg
     2761 aatacccaaa ccgatatcgt catgtcctgg ttgtcgacaa tttgcatcct caggcctgag
     2821 ccttcagtcg cagcgtcaag gatgggaggc agagagtcgt tccgaccgtc ccagttgttg
     2881 atagcgattt tcgctttcag aatgatgcgg tacgtctcat cgcttagcgt cgtatagcca
     2941 gaatcaggat catatggccc tttccagatg ccctggtcat acccaagccc gtcagtgtcc
     3001 cagctgaaat aaactccgct aattggctgg ctgactatgc gactgcgtcc gatccacaga
     3061 ccgaggatgt cgagctgtac accgacagca gtatcaatat cgaaggctgt tataagccct
     3121 gacatagtgc tggacacatc aatcagcggg cgggtgctca gatctatatg gtcaaaaaag
     3181 agtggcttgg tagcgtggta gttagtgatc agttcggtgt atttgctcat gaggtcaccg
     3241 tgatactgat attcgcggtg ctacaggacg cagaagcatc ataggcaata tcaatgtttg
     3301 atgccgatac gctgccagac gacttaccga tcagcaggtc ggtaatatcg taatagcggg
     3361 cattcccgcc gctcacaacg ccgaggtttg ccggggaata aatacggctc agcagaacgt
     3421 cgtcgccaat tgttaggcca tttatataat cggcaacagc ctgtttaatc tgctcgccga
     3481 tttgagaggt atagccggta aaaactttca gggtaatggc tacgaaaatt ggcacatcgg
     3541 tagagcgcga aaaactgatg acgtgtggat taccgtaagt atccggcacc gtgacagaag
     3601 ttttaccgta agttgcggtt ccctgccctt tactcccccg gattgtttgg gctatctcgg
     3661 taacatcccc tccatcgacg atggcggaaa tagagtgtgg cggcagcccg ttgctgtcgg
     3721 ttgccccagt gtcgttctca tatagtttgt gacgtgtcac gccagcaaca ttagcgatag
     3781 caccgtcgac accttcaaac ggtgtgatcg atggtagcgc gacgctttgc ccctgccgaa
     3841 tgcgcagctc tgcgtcggtt tcggctggtg aaccgacagt agccgcaact ggattggtta
     3901 ccgacaccca acctcgggtc ggggtgttga tagtggtaat agtcccggcc agcgccgcaa
     3961 ccgaaccgct attcgcacat gtggccgtca ccagcacagt accatcaacg ccgatcgcta
     4021 cacttgcggg aaaattccag ataatgccgt ttttatcccg tgcggagcca ttcgtgatag
     4081 tcgtgcctgc cgtaccggtt aacagaaggt cagcagtaga gtttgtcgct acttttcgcg
     4141 tgatcccgtt aattttcaca ttgctgctaa gcgctgcggc ctgcgctgtc gtcggtgaaa
     4201 aagagttgta gatcccgata gcggtgttgt tagcgtcatg cacggcaaga gccaccagcg
     4261 cgaccatctg cccgtctttg ctgtctggtt cgaggtaggc atcactaccg taaatctgcc
     4321 tgaaatagct ggtcagtgta tctaggattg tctggtaatc aggcgcacta atcccctggg
     4381 cggttaccgt tgccgatagc cccagcgtgt cgaggttcaa agccatttat gcctcgcttg
     4441 ttacagtcgt ctggccgtag attgtgtcaa tggaggaagt gaaggtgacg cgacggctgg
     4501 tgccgtcata attggtatcg aaggaaagaa tcgacagaac gcccggtgta tcctgtatgc
     4561 gttcgcgtat agccaggatg tagacatctg atcgctgctt cccaagcact gactgaacat
     4621 acggcgtgcc ttccgtcaga tcgagaaacc actgaccgcg ccacagctcg aaacgggttt
     4681 ttacggcctg ggcgacacat tccggactgt cgataaggaa agtatcgtca ccctggccga
     4741 aagtgtaatc gccttcagta tcttcgcgac ggtatcgcat tattgcggcc ctccggtagt
     4801 tcccccgcca gtctgtactc cgccatgttt atgcgtggcg acactgatac cagaagctgt
     4861 tacgtcattc gttaccatca ccggcccaag catcgtcgca gtaccgccgc tttcgcccat
     4921 tccctgagac aagttgccat taatcgttac gttgccgttc agcgtgatag tcggggatgt
     4981 gattgtcgtt ccaccttcag ccgtagctgt aagctggccc ggtgttttaa tggtgatgtt
     5041 atgtcctgcg gcgacttcta caaacgccgc accatcatcg gttcgcagct gcgcggcgct
     5101 ggtactgata ccgctgattt tccgtgcttg cgactgcggg ccaacgatgg cgaacgcatc
     5161 agataagtca tgctggcgcg ggtcgacggt ctcctgaacg ccgccgctct gccaccaaaa
     5221 atcgatgcaa cggtcagcaa aaatcaggag gcactcgtcg ccttctttta ccggaaaggt
     5281 cagcgtgcaa ccgccgccgc gcgggaagat gaccggcaca tccaccagcg gttttaattc
     5341 ggtggatcca tcgccaacaa taccgcgtag cgccacctct actgtgcagg taacagtctc
     5401 aggatcgaac gactgaatga tgccgggcat cgctacgcgc atctgggtag acaccgaatc
     5461 ggcaatggcc tgcgcggtct gctgctcacc gccgatctgt gattgagttg gaattggcat
     5521 aaaaccccca taaaaaaacc agccgaagct ggtttggtat caattatctc ttagtaacta
     5581 agctacatca gtgtgaagta aggcttttcc gctgcaatgg cacacattca caaggtgtat
     5641 taggatatgt ttgcaacaag taagcattga aaatcaattt cagccatgaa gccgccatag
     5701 agtggacctg caccatcgca ttattgacta caggagtaac aaatgggatt tagatttcgt
     5761 aaaagaatcc ggattgcgcc cggactcgcg atcaacatta gtaaaagcgg agtaagcact
     5821 tcaattggtg gtaaaggttc caccattaac atcgggaaaa aaggcgtaaa gatgacaaat
     5881 ggccttcccg gcacggggct gtcgcatacc actaaccttt actctcccgg gaaatcgaca
     5941 gaaaaaaaac aacttactca taagcaaaaa ataattagaa acatcctgtt cgtaattatt
     6001 gtgtttataa taattaaagc tcaatatttt tgacgcatgc ccgcctatct ggcgggctat
     6061 tttacttttc ggcagtcgta tgttgcatac tgacgcggcg aatccatgct ggcttgcagc
     6121 cactgtgcgt tgagaatggc tttgccgttt cgattgatgt actcaagacc aacccatctt
     6181 ccaggctgat cggtagccat acgccaatcc atcttgatat tgttataatc gcctttgttt
     6241 ttcaggaagg tgattttttg catttctggc tttgcgccat tgattctggc taagccatca
     6301 tctgcccaat ggattttaaa atcaccacat tgctgatccg caaaagcggg tgctgatata
     6361 agcaccgcga acacggtaac agtgcaaagt aaatgtctca cggccccacc tcactaattc
     6421 gtcgtccctt gcattgcttt cgggctataa agatcacgag cgccacgcgc aaaacacatc
     6481 aaatccatgt accacgcctg acctctggtg tcgccagtat agtcgatagc tttgacgata
     6541 taaacgccat ccgtcgcaat gctggcagcc tgtgacgtcg tgccggtcag cacacggttg
     6601 ccgttctctt ctgtttcggt gatacgcccg ggcgactgtg ctatttcgct attgccgagc
     6661 gtggcgcggt acaccgaagc ctgatcgagc tggataagac cattaatacg gatgtttggg
     6721 tttatcaggc atcgcacgtt tactccgcca cccatcgttt gttgcggcat accgatcagg
     6781 ccagtattgg cattcaacac aatggcttcg tgaatatatt tatcctccgg caccatctgg
     6841 acctgaccat ccaccagttg ccatgtcgct ttgcactgcg cagcaatatt atccatcacg
     6901 ttgcggctgg atgagtaaat cgcgcggcca cgaggaaaca cggtatcagg aaaatcgccg
     6961 gtaatgccct gtgtcacgcc gaacgcgttg aaatcctgca tcgtcgcccg gtgcagatcc
     7021 gcaacggtat atccagcggc aagcgtggtg atggtggtcg catagaggaa cgcttcgtga
     7081 tcaccaatgg cctgaatcaa cacccaggaa tccgtaatgt tgtccttccc ggtgacggtg
     7141 aagcgaatat caccgtcaaa aatcaggccg tagttctgac cgttcacctg ccctatctgg
     7201 tctggtgaaa tctcccggga cacaccaacc tggctcgcat caacatccgg cgctataccg
     7261 tcatacccgg caatgatgcg aattttggca aactcctgcc caagtatctt gttcgtggta
     7321 tcggtcgaaa ggttgtaaat tttcacgttc gccacgcgcg gccagcgtgt gtctgcccac
     7381 tcgatctgga acgtgacctt aaagtcagac agggaaacgc cctgcccgtt ctggtccaac
     7441 agttgcagct caaaatggcg catccagtta agagacattt ctactcctgt acgaaaatga
     7501 ggtggctgta tgtgccgagg ttggttttgg tgggctcgtc tggtgcgccc ttatcggtcg
     7561 ccaccaccag cgcgccatca atgccaagct gtggatattg tcgtaaaagg tttacgccgg
     7621 tcagtagagg tacgccagag agaagcgcgg caccgccgct atccatcacg tccataatcc
     7681 agccagccgc atcacgccag atgatcctga gtgtatacgt ggtattgccc agcaaaactc
     7741 ggaactactg attgtcagga gaaagcggta tttcgttaaa ctgcatatca tcccccgaat
     7801 gctgatgtaa cgcttccgcc cagctggctc agcaaggatt cgtttggcgg tgtagtggat
     7861 ttcatcccgg aattctgcac cgccgatgtg ctgacgccat cctgcatatc tgatttatct
     7921 gcaacgctaa cgctctgcgt ctgcgacatg atcacttcac gcagggtaag cgtgcagttc
     7981 agcacgttct cgctggtttt atccgttgtc acctcgatgg ctcgcaccag catattgctg
     8041 taaacccgct ttccggtcac tacatcgaac ggcaccctag aggactggag atccagtagt
     8101 tgctgatagg tctctttcgg gctaagtccg gcgctgaggc cgattgacga tgtatcaatg
     8161 aagtccagca acgaaccgcc acctgcgaag ccgcattcca tcgtgacttc gctggggcgc
     8221 ttatacgcat gatcggcgat gaaacccgac gcgctattcg ttgttggctt ctccaccggg
     8281 tgctcagtaa tttcgagcgc atcagaatgc ttttcagaaa caaccacgct ggggattaat
     8341 attccaattc gccgggattg ctggcgaaaa attgctgata aaatatccat tatctcggtc
     8401 ctgcggggag ttgctgggtt aactgtgaat tcacgccctt ttgacggtca acagtcaaac
     8461 gggcagcctc gcgcggatcg aaaacgccgt ggatgttaat attcgtttcc tgctgaatca
     8521 ccggggcgct ggtgggcata ttgctcatta ctttcggaat gtagttgcgc gtttcctgcg
     8581 gcattagccc cattccataa cgcttaacat tcccgatccc ccagttatat gatgccagtg
     8641 ctttgctaag gtctccgccg ttctgccgca acagctggct caggtactta gcggctgcct
     8701 gagctgactt ttccgggtcg aatacatcat tcccacgcag gcccatgtcg cgcgccgtgc
     8761 catccataaa ctgaaacaga cctttagcgc cagcgcctga aacggcgaac tgattaccgc
     8821 ccgattccgt tatggccacg ctgcgcaata acccctccgg aagccggtag agctgttcca
     8881 ggttggtaaa catcggctgc atccatccca gcagttcaga gcctgctttg gttggctgtg
     8941 gccgcttaac tgactggccg agctgctcag gctcatcatc accgaaccag ccgcgcaccg
     9001 ttcggcccac gctgcgagga tcgaatcccc agtgctcttt aatccagtcg gcggttccgt
     9061 tggcgctgtc tgttaccatc ggcatcgctg acggattttc gctgccctga ttaagcatct
     9121 gtttgccgat gctggcggca tcagcccagc ggccatcttt aatggcgttg agcaggtcgg
     9181 cgatcatgtt cagcatcttg ccgaactcac ccatttgcga aatgaagttg ctaaaatccc
     9241 actttaggga ccatgacttg gggtcaatac cgagcaactt agccagcgct tttgtcaggt
     9301 ccgtaacagt ctttttcagg tcaccaatca ttttgacggc ctgatcaatt tcagtcttcc
     9361 atttccccca gtcaatcagg cttttgccac cttctttcca cgtctggtaa tcatccaaga
     9421 gcagaccaag tgcaataata agcgtcgtta tcatgcccaa gggtgatgac atgaaagcgg
     9481 tatttagcag ccgccatgca accaacagcc cactgaacaa tgcgataagc tgctgcgtgg
     9541 ctgggttcag ctttttgaac caggcgatca caccctcaac agcttgccca gttcgccata
     9601 acacgcgggt aatcgcgtca cctgcccaga gaattccttt gatgatcttc gtgatgaccg
     9661 cttcaatctt cggccagttg tcgagtatct gcctgcggaa gttatcaata ctgccagcaa
     9721 ggccaccagc taggttagag ccaatcttgt ctttcgcctg tccaagcgtc atcgtcagat
     9781 tacgcatgga ggtcatgaaa atattggact gtttagccgc tgactcagca ttaaaaccaa
     9841 tacgctttgc cgtcaacgcg tactcagaac tgagctgccc catccctctg cgcatcgcca
     9901 tcagcgtgtt ttcatcgatg ccgagcatct gcgcgtattg cttcgcgcgg taatacggca
     9961 tgttgttgag cttttggcca acgccagtaa agatggcagc agtatcacgc atctttccgc
    10021 tggcatcgcg ggtctggaca ccaagacggt tcaggaaccc ttccgccccc ggattgctac
    10081 gcatgaagcc agccaaccct tcgagggagg acatagccga ctcggcgctg gcaccggttt
    10141 gcgatgcggc gtatcccagc gctttgatgc cctggacact ggcccccgtc cgctgggatg
    10201 cccagtaaat tttatccaga ccgttcgcga tctgagtggt aaatccgaca atgctcagcg
    10261 ctgcgccttt caccaccgcg ccgaccttca gaacgttcgc ggtaacgcct ttcagcacgg
    10321 cttcaaactt attagcgcca gcctgatcga tatcgaatcc cagcgaaaca aggaaatctt
    10381 taatcgtatc tgcgttaccg ctcattggcc gctctccatt tatctacccg ggcgtcgtta
    10441 tcctcgcgca tgtcgaggta gtcattgaga agcgcgatgc ggcaaagatc taccgcaccg
    10501 ctgttaaggt ctttctggtc aatatggaag gcaagcgccg ggcgaagaat aaaatcttca
    10561 ccgcccggca ggctgttgaa ggttattccg ctagcggggt gggcgtctcg ctggtaggga
    10621 gtccttgcaa aaaatttccc agcgagtcgg cgaccacccg cgccaccagt tgcagcatgg
    10681 taagcaggtc gatatcgtca aacgccattt cgccatgctg gcagaccggc acccagcctt
    10741 tcatgtgctc gcgtgaaaca acggaaagac aggggaacag gatagcgtcc acgtcgccat
    10801 cactcagatc ggacacagca ttggcaatct ttggcaggat agtagccatc gcgccttcgg
    10861 tgtctttgct gctgatcttc tcctgaacgc tccggaagtc cgaaaccatc ccggccagaa
    10921 ccggcaacag cttgcgggac accttcagct gttcgaaaac gctgagcttt gcggtgcgat
    10981 atttcacgcc tttaatttcg aattccatgc gttaaaactc cccgagaagc tggtcaatct
    11041 tgccgcagtc gaatacccag gcgacggttc cgccctcttt agcgttattg aaatcaggct
    11101 gtttctggaa tgcacacgaa cgcgcagtag aaatatcacc cgatgccgtg ttgcgaatga
    11161 cgatcacgtt attgccccag gtggcagagg actggctttg cgcgttatac gccagagaca
    11221 gctttttatt caccggggag gttttgagta gcgtcaccgt aatggtgcct gacttatcgg
    11281 cgtgcaggct gtgcatcacc tcgccatcgg caccgatagt catggtgttt ttgttgccgc
    11341 ccatggtctg ggtgatacct tcctcagagt tcgcagaacc ctgaccaaga tcgataacgc
    11401 cggtcggccc ggtgagcgac gcggttacat cgagaaaaga ataagttgcc atttatcgct
    11461 ccttagcgaa ccacgttgat ctgcacatcg gcataatgaa ctgcgccagc cagcttacag
    11521 gccacctgga ttaacggtgc tttgcgcgct tcacggtcgg cctgcgcctg ttcggacagc
    11581 ggttgcgcat acacgtaata gcctttggtc agcgtatcgc cggaattcag ctgtccgata
    11641 gggccaccat tccacacgcc agcagctacc agaccgttcg tgacggactg atccatggac
    11701 tgttcaacgt tggaaagcag acgggtcaca ccggcatcag tctgcggaat tttggtggtg
    11761 ctggtgtaaa gcaggttata gaggttggtc tgaacgtagt tctgcaacca gtcgagcccg
    11821 tggcgctcgt cgaagaagtc accgttcgcc atgacaccct gttgcaggat cgccgtgtcg
    11881 ttggcgtagt acacgaatac attgcagttt tttgcatcaa gtgccgatgc ctggctgact
    11941 gtcagtgttt catacccgac acccggctcc tgcttaaact tgagcgtaat cgcggtatta
    12001 ctgccattga aattaaccgt gaatgcccgg ccaaatgcag ataacgcagc gtatttatta
    12061 cccgatgaat attgaataaa actgcgtgaa tatccggcgg ttttcagttt tgatgccaaa
    12121 tcatcgctgg atgcagtctg caggcatttc tcatcgcttg tcgtaatcgc cagaatacgg
    12181 cttacagaag aggattcgat cgccgcagcc actttcagcc agtctgcatc cggaatatct
    12241 tcatcgtctg caatccccag cccataccat gaagtataat cgagcatggc attcacagcc
    12301 tgctccagcg tctcaggcgt ggcctgttcg ctgtctccct tcgttttcac ccaacgacca
    12361 acaaaaacct cctgaggttt cggtgattgt gagaaaaaca cctgcgcagc cttatattct
    12421 ggtgattcca cgccaaaatc ttttccaata tcttccgcgg cagaataacg gcgaatgcgc
    12481 tcacttaccg gaatgattgt ggacgggccg agaatgagta atgcaccaaa atttcgccct
    12541 gatgctgcac gcggcgacat gatcacatca acattaacaa cgtttgatac aggcaagccc
    12601 tgtgccatag cttaatctcc gaaaaagatg actggtgctt ccaccagcga tttaataccg
    12661 tactcgcgca caaccttccg gcgcagacgc accgtcatat cgtagcggcg gacccattgc
    12721 tgattaataa gttcagggaa gagacctgtg taatcgccaa gagacagccc cagcgcattc
    12781 agtgctgcgt tgttctgcgg tacagatata ccgtcacgaa accgggacgc atacaccatc
    12841 cccgccggac cataaaacga agccatacac tcaatcgttt catgccgcca gagctgagag
    12901 ccatcatcgg tctgtctggt gaatgccgga ctgtcatcac ctgaccatcc gataacccca
    12961 aacgcacacc agttcgtttc aaccggtagc agtggcggct gctctttctg ccagcgcggg
    13021 cgaaccatcc cggcagacag accggaaacg ttacgcatcc actggcttaa cagcctgtcg
    13081 agcgcttcgt cataatccgg atcgccactg gttggtatta accatccgcg ctctgtactg
    13141 gtgttattgc tcaaccggag ttcccccatc aaacggcatc aactcacaat gcgcctgaac
    13201 gaatccggcc ccataagctg tatacgggtc gacgaaggtc acacgataat cacggccctg
    13261 atacgtcacg atatcggcat cacggccagt ctgtccctgc gtcagtcgct cagtcgtcac
    13321 aatcagaatt gcaccgctga ttacctgccc tgcctgcata cggcggtttt ccagagagcg
    13381 atcaacagtt acgactccgg caaactgctt tttaacttca ctgtcgctgc cgatcccgtc
    13441 ctcatccacc gtttgcactc ggcgtgttac ccacaaattg aagtcgcaaa aatcggggtc
    13501 aaaaagcaca tctgttacat caagagtcgg catctttatc cctcacaaca tgggtaatag
    13561 ctctgcgata ttgcccggtg tcaattaatg gtttcgccag atcggttccg ggagattcgc
    13621 cagcaacacg ccgagcaagt tccagtgttg cccccttgcg cccccgacga gcccgggctt
    13681 caacggtgct atcagcaagc ggtgtaaagc cggtaatggt catgtaacgc ctgacgccat
    13741 tcgcggccag cgttccggcg cggttaagcg ctctttccgc acccgccgca tttccatcaa
    13801 gcgcagcctg cgccgctgct ttgagctgcg gcaccgtctg ttcctctacg gatttaacgc
    13861 cggggatcag gtgcgggcgt gggggtatgt tttgcgctgg tgagccgtat tcgttgacgt
    13921 aaccgatccc ggcattacca aacggaacat cctcacgctc gctgtcttct tccgggatgc
    13981 ccaccagcac atcctttttg gttagcgacc ggagcgcatc cagaatggcc tgagcgttat
    14041 caaccctcgt tgttacacca cttttgaaac tcatagctgg cgaccgcccg caccgaacat
    14101 cgtgatcagc tgataaaatt cagcgccata tcgggtgtta ttccagaagc ctgcgtcagg
    14161 gtttagcgtc gcgctggtgt catagctgac gcttaccttg tcaacggact tggaggactg
    14221 aacaccattg gttgaaccgc ccgggccgcc aaccagcatt gcccgactat ctgccgccca
    14281 tagcgtcatg tagtgagcca cgaacaaccc ggcaaagtac ggaaacaaat ctttgccggt
    14341 gacgttttcg ctcagcagtt catcggccag attcagacga aactggattt gcgcttcggg
    14401 atatttggca gggtcagcaa actgcgggaa gtcgcggcga aaatcactta ccgctggcag
    14461 gctttgattc tttggcattt tttacctcgt tacgcgcgtc tgtggctttg ccaacggata
    14521 cttccgcgtg cgcacgagtg aaccagtgcg tggcaacgtc ttcctccact gcatgacggc
    14581 ctttaacaaa ctcgcgccgc gaaccgtcgg gaagcgtgag cacaaacggg gtatgtacgt
    14641 gtattactgc attatttttt gccatcgggt catccttaat ggccccgcca ggggggccat
    14701 gtggctgtta aatgccatca acgtacgaaa tggtttcttt gtacactggc tcaaccgcac
    14761 ccagcttgcc gtagtaggtc gcaatctgat acagaccgcg atactggata ggaacgctct
    14821 gcaacggcac cagcggatag cggacgtatt ttttatcgtt ggtgtacgca accatgcgat
    14881 ccttattccc cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac
    14941 cgttctggtg atagctgatg gtgttggtct gaaggtacgt caacagggac tggttacccg
    15001 cagatgaaac gatgatgctg gacaacagag caaactgctc tggcgggatc agcaaatcac
    15061 gcggaaccac agagtaaccg gaagcggccc acgcatcaga cagcacctgg ttaatgcttg
    15121 cgcggatttc gtccggtgtt gagattgccc acgttttggc agcgttgttg acaggcacgc
    15181 cgtccagggt aacaaggcct ttcaggttta atgcggaatc gccaacatac acctgctcgt
    15241 cgttgtccat ctgccatttg agctgcatcc cgtcgtactt ctgggtgtca atcgggcgac
    15301 ctacctgctg agctgctgcc agctctacaa cggtccagcc cagttccatg ccccagagat
    15361 tcagcgggtt accggatttg ccgatatcca cgtttacgcc agcaatagcg gttgagtctt
    15421 tgcctaccca gtttttgcca ttcggatttg caccagtacc cgcagcggcg aagctggtat
    15481 tcgtccagct ggaaatgtca tctgcgatag agacatcttc acgcaactga atatcgcggg
    15541 tccaggtgta ccccgccagt ggcaggttca gagtctggtc gagtcgctcc agctctccga
    15601 tgagaaaggc accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc
    15661 ttaaatcttc caggaaattt ctgcattgcc gttagcatca ccggcacctg tgaattcagc
    15721 gttggtcagc accacatttt tgccactgac tgacgtggac atgaatccac ccagcggcac
    15781 tttgatggat tcatcagtgg agacgacaac gtataccggg tcgccttttt tgatggtgct
    15841 ggcatcaaaa tcagaaccga gattaacggt cacgtagcca cgcttcatgg cgtcgcccgg
    15901 gaagttcttg cctgttccca cctggcgaac catgtccggc tgcgaagtgg tcggataagg
    15961 gcgcacgtag atccccttca ccttgtctgc ggtatcgcca tctgccagcg gcacgaaaaa
    16021 accgtcagca tcgtatttac cagccagccc ataggcagca aaggcgttat cggatttaag
    16081 gaccaccggt tcgacggtta agtcctgcgg gcgagagata gccccggcaa tgccaacagg
    16141 catccggtac agatatgcag tcattggatt atcctttgcg gttagaccag aattcagcgt
    16201 tttgtttgtt cagggaagcg atgctggtca tgcccatgtt tggacgttgt gcatcgcccg
    16261 tggtgctgcg ggtgtttcgc cctttggcaa tctcagacac agcgttaaac gccatatcga
    16321 ccgattgctt aggcaatttg cggatatccg catcaccgac aacctggcga accagcgttt
    16381 tgtcagcaga agccagaacc tcgcgtttga acacggtcgg tttcatctta cggctcagat
    16441 cgatacccgg aacgataact tcggcacgcc aggctgagtc accagtaatc gtggtttcct
    16501 cttcatcgtc ctcgccgtca ccggtcggat tatcgtcagg tttattatcg ttatcgcccg
    16561 tggcatttcc ttccagctta gccagcaggg ctttcagtaa tgttttgagg tcatcatcac
    16621 tgtcgccggt tggacctccg cccatctctg gtactttgtc cggtagcggt tgctgcgggg
    16681 acaggttgat gttgagatta acgccctgcg gcaaatcccc ctcatctcct gtaaccgatg
    16741 cgggagccga ctccaccagt tcgttcatgg tgtcagcgtc acccgttttg atggccgtgc
    16801 gcatgcgggt ccaccagctt ttcttttgat ttgccattgt gtctctgtct ccaattgcac
    16861 aacgatttcc ggctctgcct ttggggacaa gagccacatg gtttccggta atatcgacct
    16921 gctcggcttt acctggctcg gtctgctcgt actccgcgtc atagccgcac gacacttcac
    16981 gcaggccatc ttcgataagc tgaatggcgt tttcgtcttt gacgataagg tcagccagca
    17041 tcaaatcaga gtggtcaccc gtcccgcgcc gcacattctg aagatgcccg acagcaagct
    17101 ctttccagtt ctcgggattt accagccgca cattcccgtt ttcatcttca ggatgcagga
    17161 tcgtgatgct catcccttcg aatgaggcga gcgtggccgg atggaatacc tgctcaggag
    17221 agcgcgttac gactatctca ccgagcttgt cgggtttgag gtttggcaga tcggcagcgc
    17281 cgtagagctg tttacccgtt cgacctatcg gcacgtcttt gcacagcagc gagccgtcag
    17341 ccagctgata gcgggtttcc cccagccggg tattgaaaaa atatttcatg gtttacctgc
    17401 gattcaggcg agataagaat gagggttggg aaaaacgatt tctttataac agcgacaatt
    17461 cgggagctcg ccagcgtgac cggtcatgcc atcaagcgtt ggaggtttgc cccattcgac
    17521 aaacttacct tccatctccc gatgagaatg ccggacgtcg ccatcttcgg ctgtacgcca
    17581 gatataacca ttcgagccga ttgacagcgc acgcgcctga tcgagcgcgc cggttgcacg
    17641 tccaagctcg gtacgggcga taaggttcgc tcgtgagcgt gacacgtcac cggacgctgc
    17701 tatctctttc gcgaatggtt cagcgcggcc accagccaca acggcctcga tggccttgtt
    17761 ctgaatgtca tacacccgat cggcggcctc aagaggcagt gacttgatat acttaatttg
    17821 ctcggcgacg atggatttca tcacctggcc taccggggcg cggtcgacca tgttgcgcag
    17881 ctctgcgctg atgttccggc tgtgctgacg ccactgcttt tcattctggc gtgcaatgtc
    17941 ggcggtaaag ttctcagcaa ccttcgtcgc ccagggggtg atgatttcgc tgtagcgctc
    18001 cagcgcatcc attatttcgg taacgctatc atttgaacca tcgtagcgcc catttacgat
    18061 atccccgacc gcccgcgcta tctgccgtag gctcgttcga tatcggatct ccgcctggcg
    18121 gctctggcgg tttgtcgcca agttcgccga tgcctggcgg cgcttcgtct tcggcattct
    18181 cgatatcctc gtcggtaatg gatgccccga tgccggtgac gtcagagttt tcgcgcaggt
    18241 cggtcatcgc cgccttacgc gtcatcaatc cgtcgcccag cgcggtacta atcgcgttgg
    18301 tggtgtttac ggccaccgtt gatcggtcaa cgtctgacat ttgccatagc gggttaaact
    18361 caaacgtgaa atcgtccggc agcggctttc cgagttccga gcggtgcata atgtccagta
    18421 tccggcgcat cggcagccgt aagcggcgct cctgcaatga gctcacccgg tcgtaatagt
    18481 tggcgaggtc tgcatcacca gtagagaagc ctttcgggga ttgaccgaac aaccgcacca
    18541 gcgggatacc aacggcaccg ctaatctgct cagcaaactg cgaaagaatg tcatccagac
    18601 cactaaagct gtactggtgg gtttcgaact tatcccgcga gtccatgagc gtcatacctt
    18661 cattgctctg gaactggcgg atcaggtcga tgttcttcag caacgcctcg aacgccgggc
    18721 ctccaagcgc gataagctcg cgcaacttct ccacgctata ggtacgcaga tgtgctttgt
    18781 agaccagctg cgccgcgccg acagtggcgc tgtcgaacgc agtaagccga tcccagatac
    18841 gctctacaac cgacattccc cattcgtttt cggtcatctt ctgctggaat ggcagcgtca
    18901 ccccgtcgaa gcgaatcagg cggctgtgat ggatgcgcca ggccgggatg cccgttgcag
    18961 tggtcaccac gtcgtaaaac tcaggtttgc cgagatctgg ccccatctct ttaatgcggc
    19021 gggtcaggac cgggttaatc atccagcggt cgagcgggag aatgccctta aacttgcctt
    19081 ctccaatggt ttcgagccgc agcggggtca ttggtgcctg cccctcgatc atgatgaagc
    19141 cgaccgcgcc gccgtagagg cgagaccatt tcagcacgtc gttcagcgca tcccagattt
    19201 gcaactcatc cagttgtgat tcgagaatgc cacgatcttt tgcatcaatt tccgaagtga
    19261 tgcgaatgcc tttgcgggtc atatcatccg ggatagcatc gaccgcttcg ccgataatcc
    19321 aggacgaacg ataggaccat tccaccagca tgcggttacg actggtgaaa ttagcccggt
    19381 aggtggatgc tgagtgctgg ttaggtgtct gcatccccac gcgggcaaca aagttctcat
    19441 agccatcagc ggtggcctgt gccgttcgcc gcgtggcttg tttgtttcgt gccatcaggc
    19501 ctgtctccct agcagctccc agatgttcag ggctgaattc attggcgcgt agctgatcat
    19561 caccgagtcg gcaaggtttg gcgaccgggt tccatcaggc tgtttatcaa taacgatttt
    19621 tcccacacca ttaatggaat aggtcggctg cgaaagctcg atgatgagtt tatctttgag
    19681 tgccatgcta ctgctgattg agatgatttc gtccgggttg taatccatac cttcaaccac
    19741 ggcgcgccag gtattctgaa aaagtttacg taaccgccac cagctctggg ctttggcgtt
    19801 agcgaagaag tccttgttca ggcgggcggc ctgtccgttg tcaccgcgca ccgcttcgtc
    19861 gtccggatca aataccgcgc cactacctcg aaacggtgtg gcgagtattg acggtcggcg
    19921 cgcagcgtta cgcagttcgt tgatagcgcg tgcatcgccg cgaacgccag cgcccaaccc
    19981 gtcctcgtca aagcgaaact cttcgaggtt gtcctgttcg caaaagccga aaaccttctc
    20041 gacggactga taaatgtcgc tgcccacacc ggaccattcc cgcacattct ccaggaggaa
    20101 gccatgacgg gtggaaaagg aatttttgtc cctgccttcg tcggcgacgt ccatcgcgcc
    20161 cagtcgcttg cctgttggct ggatgcccag cttgatatgc gcgtcgacag cagcctgtac
    20221 ccattcggat ggaatcagga cgccttccgc tgatgcactg tagttcaggt caagttcctg
    20281 tgccaccacc accggattgt cgattttctc gcattccctg cgataccact cttcatcctt
    20341 gcgaggatca tcccgccagt ggaatgtgaa taccggtatc ttcccgccat gacgcttctg
    20401 agcgaacggg ttcgccatgc cgttaactga actcaggtca atacggcaac gcgtcgtttg
    20461 tgacaacgcc gcatcaatca gcagaggacg ctgaaggaat gcagactcat caaccagata
    20521 aagcgtggta cggtcaccac ggccaatatt atcgccagcc tcacctttga taacggcacc
    20581 agtttcagga aactcaacgc gcatgtatgg tgcgtgcttc ttttcgtccc acgaacctcg
    20641 aaactctaca ggtagtgttt ccacgaactt gcgcgccttc cagaacaatg ctttcgggtc
    20701 accggtgctg tcgacgtatt cctctttacg ggagccgaaa ccgataacca tttctttgtt
    20761 gaagagacaa agcgagcatg ccagcccgat cgcggtccaa ctgagcccca tttcacggga
    20821 tttttcggta ataccattct cccgattacc ccagcgttcc ataatccagt ggatccactc
    20881 ctcctgctta gggaagagta aaaacggaat ggtcaccggc aggccataat caatattacg
    20941 cgggtccgtt gtcatgcccc agtcgatgat gaactgagcc ggattggttc ggtaaaactg
    21001 ttttagtgca ggcaatattt caggattctg gcgaatgcgc tgtaggcgtt ccatccgcca
    21061 ttcaaaaacc atctggtaat caggatgttt aaaatcgaag gggaatggta acggcatact
    21121 tagcccatca tttttctata cgcctctgca gcctgctccg gcgttaagtt ggtaatttct
    21181 gttctgactg gtcctccgtc agcaccagtc acttcatttt tgacattgtc tttaaacgcc
    21241 tgaacagaaa catgacgccc gagcaactca aggtttttaa ccttatcagg ccatttgatt
    21301 ttcttcagga gagcagcact atctgctgat gccatctcga cgacatccat tcctgatagc
    21361 gttgtacgcc acaccttagg ccagtcttta atctgcttca gttcgccatt ggcaagcaga
    21421 atgtcaagca cgtccatctg atcgatttca gtcagccgtc gcaatacata ggcggcgtct
    21481 atacctacct cttcattgcg ttcagccttt agctcagcaa tgcgatctgc tatgtcaggt
    21541 tttgacaggt tctcgctacc agtggcgcga gcagttttct cgctgtaacc cgccctgata
    21601 gctgcctgag tggcgtttaa atcttttaaa tattccctgc agaacagttc ctgcttaggt
    21661 gtgagctgtt tcaaatcaca cctccgaaac attgctccaa tttctatgat taacgatgtc
    21721 agcaatcatg gtagtggata cgccatatct ctttgctaaa gacctaagcc cattacctac
    21781 actcccgcgc acatactcgc ggcgaatgca gatgacatcg cactctttta atttcgccat
    21841 tccgttcttt tcacctgaac ggtcagggta tgagtttctg cctcgccttg acctgtcacg
    21901 attattgtca gcgacagtac ctataatcag atggtcagga ttcacgcatg atggattatc
    21961 gcatttatgc ctaacaacaa ggtccttg
//
LOCUS       DAEEGA010000063        21505 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEEGA010000063 DAEEGA010000000
VERSION     DAEEGA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21505
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.063"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(37..2106)
                     /gene="glyS"
                     /locus_tag="KLH46_002669"
     CDS             complement(37..2106)
                     /gene="glyS"
                     /locus_tag="KLH46_002669"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBE0379298.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="KLH46_002670"
     CDS             complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="KLH46_002670"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBE0379299.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3122..3421)
                     /locus_tag="KLH46_002671"
     CDS             complement(3122..3421)
                     /locus_tag="KLH46_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBE0379300.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3596..4591
                     /gene="wecH"
                     /locus_tag="KLH46_002672"
     CDS             3596..4591
                     /gene="wecH"
                     /locus_tag="KLH46_002672"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBE0379301.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4633..5070)
                     /locus_tag="KLH46_002673"
     CDS             complement(4633..5070)
                     /locus_tag="KLH46_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HBE0379302.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5116..5457)
                     /locus_tag="KLH46_002674"
     CDS             complement(5116..5457)
                     /locus_tag="KLH46_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379303.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="KLH46_002675"
     CDS             complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="KLH46_002675"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBE0379304.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="KLH46_002676"
                     /pseudo
     CDS             complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="KLH46_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8840..9832
                     /gene="xylF"
                     /locus_tag="KLH46_002677"
     CDS             8840..9832
                     /gene="xylF"
                     /locus_tag="KLH46_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0379305.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9910..11451
                     /gene="xylG"
                     /locus_tag="KLH46_002678"
     CDS             9910..11451
                     /gene="xylG"
                     /locus_tag="KLH46_002678"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBE0379306.1"
                     /translation="MSYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11429..12610
                     /gene="xylH"
                     /locus_tag="KLH46_002679"
     CDS             11429..12610
                     /gene="xylH"
                     /locus_tag="KLH46_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBE0379307.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12688..13865
                     /gene="xylR"
                     /locus_tag="KLH46_002680"
                     /pseudo
     CDS             12688..13865
                     /gene="xylR"
                     /locus_tag="KLH46_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
     gene            complement(13973..14797)
                     /locus_tag="KLH46_002681"
     CDS             complement(13973..14797)
                     /locus_tag="KLH46_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBE0379308.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="KLH46_002682"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="KLH46_002682"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBE0379309.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="KLH46_002683"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="KLH46_002683"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBE0379310.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="KLH46_002684"
     CDS             complement(18729..19202)
                     /locus_tag="KLH46_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBE0379311.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="KLH46_002685"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="KLH46_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBE0379312.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLSAIT"
     gene            20353..21351
                     /gene="yiaK"
                     /locus_tag="KLH46_002686"
     CDS             20353..21351
                     /gene="yiaK"
                     /locus_tag="KLH46_002686"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HBE0379313.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21363..>21505
                     /locus_tag="KLH46_002687"
     CDS             21363..>21505
                     /locus_tag="KLH46_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBE0379314.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5398 a         5488 c         5284 g         5335 t
ORIGIN      
        1 gcctgccaga tggcaggctt tttaataacg gcgttattac tgcaacagcg aaatatccgc
       61 aacgcgcagg aacagttcgc gcagtttctc cagcatggtc agacggttga gacgcaattc
      121 tttgtcatca accatgacca tcactttatc gaagaaagca tcaaccggct cacgcagctc
      181 tgccagttcg accagcgcat cctggtaacg accttccgca aagtacggct ccagcttgtc
      241 acgtagcacc acaacctgca tcgccagttt aatttcttcc ggctctttca gggtagaggc
      301 attcacgcga tcgctcagca cttcgtcaga tttcgccagg atgttagata cacgtttgtt
      361 cgccgccgcc agtgctgcag ctgcttccag ggtacggaag tgcgataccg ctttcatgcg
      421 ggcatcgaaa tcagccggac gagtcggacg acgcgccagt accgcctgga tggtgtcaac
      481 ggtataacct tcgtcctgat accaggcgcg gaagcgaccg agcataaagt cgataacatc
      541 atcaactacg ttggcattag tcagcttatc gccatacaga cgcaccgctt cttcggtcag
      601 cgtttgcaga tcaaggttga ggttcttctc aacgataatt cgcagcacgc caagtgcggc
      661 acgacgcagc gcaaacgggt ctttgtcgcc tttcggatgc tgaccgatac cgaagatacc
      721 cgccagagta tccatcttat cagcaatcgc cagcgcacaa gctaccgggt tggacggcag
      781 gtcatcaccg gcaaagcgcg gctgatactg ctcattcagc gcgacggcta cgtcttccgc
      841 ttcgccatcg tgacgcgcgt agtgcatccc cataacgcct tgggtgtcgg tgaactcgaa
      901 gaccatgttg gtcatcaggt cacacttaga cagcaggccc gcacgggttg cgtggttaac
      961 gtcagcgcca atctgttcgg cgatccagcc agccagcgcc tggatgcggt cagtcttgtc
     1021 acgcagcgta cccagctgtt gctggaacag cacggtttgc aggcgcggca ggttatcttc
     1081 cagacgtttt ttgcggtcgg tgttgaagaa gaactcggca tccgccagac gtggacgaac
     1141 gactttctcg ttaccagaga taatttgctg cggatctttc gattcgatgt tggcaacgaa
     1201 gataaagttc ggcagcagtt tgccgtcgtt cgcatacacc gggaagtact tctggtcacc
     1261 tttcatggtg taaaccaggg cttcagccgg caccgcgagg aatttctctt cgaattttgc
     1321 ggtcagaacg accggccact ccaccagcga agccacttct tccagcaggc tttcgcttaa
     1381 gtcagcgtta ccgccaatct tacgcgctgc ttcttcggca tcggctttaa tcttcgcttt
     1441 acgttcttcg taatcggcga tgactttccc gcgctcacgc agaatttccg gatactgatc
     1501 ggcgttgtcg atggtgaatt ccggttcgcc cataaagcgg tggccgcgaa tcacgcgatc
     1561 ggactgaatg cccagaatgg ttgccggaat gactttgtcg cccagcagca gggtcacggt
     1621 gtgcaccgga cgcacgaagt gcacgtcgct tgcaccccag cgcatcagtt tcgggatcgg
     1681 cagtttcgcc agcgaagtcg caaccatatt cggcagcagt gcttcggtgc tttcgccctt
     1741 cacatgggcg cgatacagca gccattcgcc tttatcggta gtcagacgct cagcctggtc
     1801 aacggtaata ccacaaccac gcgcccaacc ttctgccgct ttgctcggtt ttccttcagc
     1861 gtcgaacgcc tgggcaatcg ccgggccgcg tttttcgatt tcacgatctg gttgtgcttc
     1921 cgccaggtta gctactttca gcgccagacg acgcggagca gcaaaccatt gaacggtgcc
     1981 gtgtgcgagg cctgcgttat ccagctccgc agtaaagttc gcagcaaagg actctgccag
     2041 gctgcgcagt gcttttggtg gcagctcttc agtgccgatt tccaccagaa aagttttctc
     2101 agacatagcc gcctcttact tatctttgtt gcacatcggg aagccgaggg cttcacggga
     2161 agcgtaatat gcttctgcca ctgctttggt cagggtgcga atgcgcagaa tgtagcgctg
     2221 acgctcggtg acggagatgg ctttacgcgc atccagcagg ttgaagctgt gggcggcttt
     2281 cagaatacgc tcgtaggctg gcagcggcag cggattttcc agcgccagca gctgctgcgc
     2341 ttctttctcg tactgctcaa agcaggtgaa caggaagtcc acatccgcgt attcgaagtt
     2401 gtaagtggac tgctccactt cgttctgatg gaacacgtcg ccgtaggtgg ttttacccag
     2461 cgggccgtcg ctccagacta ggtcgtaaac gctgtctacg ccctgaatgt acatggccag
     2521 acgttccaga ccgtaggtga tctcgccggt aaccggttta cactccagac caccaacctg
     2581 ctggaagtaa gtgaactgcg tcacttccat gccgtttagc cacacttccc agcccagtcc
     2641 ccaggccccc agagtcgggt tttcccagtt atcttccacg aaacggatgt cgtgaatagt
     2701 cgggtccatg cccagctctt tcagagaacc gaggtacagc tcctgaatat tgtccggcga
     2761 tggcttaatg accacctgga actgatagta gtgctgtaaa cggttggggt tttcgccgta
     2821 gcgaccatcg gtcgggcgac gagaaggctg aacataagca gccgccatcg gttctggccc
     2881 cagcgcgcgc agacaggtca ttgggtgaga ggttcccgcg ccgacttcca tgtccaatgg
     2941 ttgaacaatg gtgcagccct ggcgagccca gtaatcctgt aaggtcagga tcaagccctg
     3001 gaaggtcctg gtatcaaact tttgcatatt atttcgtgct ggatacgtgt ggatttaaag
     3061 gaagggatca gtatacccgc tggatggaag atatacagta cgaaacggga aaaagcaggg
     3121 cttaacgcat ggaaagatgc aaaaaatggc catctgcgtc aaaagaacag acaaactgct
     3181 ctttacggaa ggtataaccg cgcatttcat agcttccctg gaactgttcg aaggcggtga
     3241 cgtcgatttt ttgctcgccg gtgttataac gttttgccgc ctgatccttg cataacgctt
     3301 ccatattcaa cgaacgcaga ggatcaacct ttacccgttg tgctttctgc acgggtgacg
     3361 gtgtagaaca acccactagc accataagcg ccattgccgg aaagaaagcg ttcatcatca
     3421 ttaaattacc gtctggtttg caggtgagtc ttattattta tatcggtata gtggctgata
     3481 tcttacgaat ttccttaagc ctctataggc tgtagtgata aaactcagat tattccagtg
     3541 cgccaaccaa atgatttatc ggtcagactt tttttaacca gatacagaga cacagatgca
     3601 gcccaaaatt tactggattg ataacctgcg agggatagcg tgtttaatgg tggtgatgat
     3661 tcacaccact acctggtatg tgaccaatgc tcatagtgtt agccccgtca catgggatat
     3721 cgccaatgtt ctgaattctg cctctcgtgt cagcgtgccg ctatttttca tgatttccgg
     3781 ctatctcttt tttggcgaac gcagcgccca gccgcgccat ttcttgcgta tcggcttatg
     3841 tctgattttt tatagcgcaa tcgcactgct ctacattgcg ctgtttacct ccatcaatat
     3901 ggagttagcg ctgaaaaacc tgctgcaaaa gccagtgttt taccacttgt ggtttttctt
     3961 cgcgattgcg gtgatttatc tggtttcacc gctgattcag gtgaagaacg tcggcggaaa
     4021 aatgttgctg gtgctaatgg tggtgattgg cattatcgct aacccaaaca cagtgccgca
     4081 gaaaattgac ggttttgaat ggctgccaat taacttatat atcaatggcg atacttttta
     4141 ctacattctg tatggcatgt tgggccgcgc tatagggatg atggacacac agcataaagc
     4201 actgtcgtgg gtgagcgccg cgctgtttgc gacgggggtt tttattatct ctcgcgggac
     4261 attatatgaa ttgcagtggc gcggaaattt tgccgatacc tggtatcttt actgtgggcc
     4321 gatggttttt atctgcgcaa tcgcgctatt gactctggtt aaaaacacgc tggatacgcg
     4381 taccattcgc ggacttggct taatctcccg ccattcattg ggtatatacg gattccacgc
     4441 cttgattatc catgcgctgc gcacccgggg aattgagctt aaaaattggc caatactgga
     4501 tattatttgg atcttttgcg cgacgttggc agcgagtttg ttactttcta tgctggtaca
     4561 acgaatcgac agaaacagat tagtgagtta agtaaaagcc cgttcacatt ggactgaccg
     4621 ggcttacgtg aattattcgc tgtattcttc ctgggtaacc tgtgtttctt ttggcgggtt
     4681 tattccggca tcacgaatat tcttctgcac cgctactgca ccaaacaagc ttaagaagaa
     4741 agccagtcca taaaaacctt tttcactgag taataatgtc gcattccaca gacctaccat
     4801 cagtagagca acagagataa taaagacagt caggcaggtc atataataaa tggaagtggt
     4861 cggtatgcct tcatacttgt cccgaacggt cttttgataa gacgcagcag aaaacagtcc
     4921 taacaccagt acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag
     4981 ccctaacagg taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc
     5041 cggtgaatag gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta
     5101 agagctttat gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa
     5161 aaaggcgatg gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat
     5221 aaacaagaga ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc
     5281 cagttgcccg agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa
     5341 cacgccaaga aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag
     5401 ataaaccagc gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac
     5461 gatctccata tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat
     5521 gccattaaca aatgattttc agaataaatt catactaaat attaattaat tagtgagata
     5581 tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg
     5641 cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg
     5701 ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga
     5761 tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc
     5821 cccccccgta cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta
     5881 ctcactacgc gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg
     5941 cacgacatcc atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag
     6001 ttcattgggg ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt
     6061 gtgtggtgta cgctcgccgg aaagataagg cagaaaccaa actggctcgg cactttcatc
     6121 agcctgttga gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc
     6181 ccaatccaga cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag
     6241 cgcatggcaa aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc
     6301 aaaatagacc cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac
     6361 accaactgca ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc
     6421 ccacgctttc gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc
     6481 gggcatctgg tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt
     6541 tgcgacatcc agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt
     6601 catacgcaga cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc
     6661 cggctcatgc cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt
     6721 gccggtaatc actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca
     6781 gcgcccgtcg ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt
     6841 tgctccatgc atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc
     6901 gcccagagct ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga
     6961 ccaaagtgga tgcgggcgcg aaacggtcag cttttccgtt tgcgaagcaa ccacctcacc
     7021 ctgctcgttg agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat
     7081 atcgatcgtt ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc
     7141 acagttagcc gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg
     7201 cgaccactct gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc
     7261 agtgacattt ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc
     7321 tgcgcgatgc gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc
     7381 agtgccatcg tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt
     7441 tgacgacgta ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga
     7501 atttcataca tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag
     7561 cccagttgcg catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg
     7621 gtggctattt catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc
     7681 agtttaatct ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg
     7741 taatcatatt gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctag
     7801 aagccgattt tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc
     7861 tcctgacgca agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca
     7921 tagttttcac cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc
     7981 cagctgaaga cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta
     8041 aagcagttag cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg
     8101 acatcaacca tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa
     8161 acatccacat cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg
     8221 acatctgctt tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta
     8281 aacgccccca caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg
     8341 gcaaaacgca agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga
     8401 cggaatgcta acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca
     8461 aaataggctt gcatattgaa ctccataatc aggtaatgcc gcgggtgatg gatgatgtcg
     8521 taatattggg cactcccttt caattgctca attatgttat ttcacactgc tattgagata
     8581 attcacaagt gtgcgctcgc tcgcaaaata aaatgggatg atgaaactgg gtaattcctc
     8641 gaagataaaa atgcaataag tacaattgcg caacaaaagt aagatctcgg tcataaatca
     8701 agaaataaac caaaaatcgt aatcgaaaga taaaaatctg taattgtttt cccctgttta
     8761 gttgctaaaa attggttacg tttatcgcgg tgattgttac ttattaaagc tgtcctctaa
     8821 ctacagaagg ccctacacca tgaaaataaa gaacattcta ctcacccttt gcacctcact
     8881 tctgcttacc aacgttgcgg cacacgccaa agaagtcaaa ataggtatgg cgattgatga
     8941 tctccgtctt gaacgctggc aaaaagatcg agatattttt gtgaaaaagg cagaatctct
     9001 cggtgcgaaa gtatttgtac agtctgcaaa tggcaatgaa gaaacacaaa tgtcgcagat
     9061 tgaaaacatg attaaccggg gtgtcgatgt tcttgtcatt attccgtata acggtcaggt
     9121 attaagtaac gttgtaaaaa aagccaaaca agaaggtatt aaagtattag cttacgaccg
     9181 tatgattaac gatgcggata tcgattttta tatttctttc gataacgaaa aagtcggtga
     9241 actgcaggca aaagccttgg tcgatattgt tccgcaaggt aattacttcc tgatgggcgg
     9301 ctcgccggta gataacaacg ccaagctgtt ccgcgccgga caaatgaaag tgttaaaacc
     9361 ttacgttgat tctggaaaaa ttaaagtcgt tggtgaccaa tgggttgatg gctggttacc
     9421 ggaaaacgca ttgaaaatta tggaaaacgc gctaaccgcc aataataaca aaattgatgc
     9481 tgtagttgcc tcaaacgatg ccaccgcagg tggggcaatt caggcattaa gcgcgcaagg
     9541 tttatcaggg aaagtagcaa tttccggcca ggatgcggat ctcgcaggta ttaaacgtat
     9601 tgctgccggt acgcaaacta tgacggtgta taaacctatt acattgctgg caaatactgc
     9661 cgcagaaatt gccgttgaat tgggcaatgg tcaggagcca aaagcagata cctcactgaa
     9721 taatggcctg aaagatgtcc cctcccgcct cctgacaccg atcgatgtga ataaaaacaa
     9781 catcaaagat acggtaatta aagacggatt ccacaaagag agcgagctgt aagcgttacg
     9841 ccccggcgcg gagcgggggc gtgatttctc tccatgccgc gtgaatgaat tggcttaggt
     9901 ggagtcgtta tgtcttatct acttgaaatg aagaacatta ccaaaacctt cggcagtgtg
     9961 aaggcgattg ataacgtcag cttgcggttg aatgctggcg aaatcgtctc actctgtggg
    10021 gaaaatgggt ctggtaaatc aacgctgatg aaagtgctgt gtggtattta tccccatggc
    10081 tcctacgaag gcgaaattat ttttgcggga gaagagattc aggcgagtca catccgcgat
    10141 accgaacgca aaggtatcgc catcattcac caggaattgg ccctggtgaa agaattgacc
    10201 gtgctggaaa atatcttcct gggtaacgaa ataacccaca atggcattat ggattatgac
    10261 ctgatgacgc tacgctatca gaagctgctc gcacaggtca gtttatccat ttcacctgat
    10321 acccgcgttg gcgatttagg gcttgggcaa caacaactgg ttgaaattgc caaggcactt
    10381 aataaacagg tgcgcttgtt aattctcgat gaaccgacag cctcattaac tgagcaggaa
    10441 acgtcggttt tactggatat tattcgcgat ctacaacagc acggtatcgc ctgtatttat
    10501 atttcgcaca aactcaacga agtcaaagcg atttccgata cgatttgcgt tattcgcgac
    10561 ggacagcaca ttggtacgcg tgatgctgcc ggaatgagtg aagacgatat tatcaccatg
    10621 atggtcgggc gagagttaac cgcgatttac cctaatgaac cacataccac cggagatgaa
    10681 atattacgta ttgaacatct gacggcatgg catccggtca atcgtcatat taaacgagtt
    10741 aatgatgtct cgttttccct gaaacgtggc gaaatactgg gtattgccgg actcgttggt
    10801 gccggacgta ccgagaccat tcagtgcctg tttggcgtgt ggcccggaca atgggaagga
    10861 aaaatttata ttgatggcaa acaggtagat attcgtaact gtcagcaagc catcgcccag
    10921 gggattgcga tggtacccga agacagaaag cgcgacggca tcgttccggt aatggcggtt
    10981 ggtaaaaata ttaccctcgc cgcactcaat aaatttaccg gtggcattag ccagcttgat
    11041 gacgcggcag agcaaaaatg tattctggaa tcaattcagc aactcaaagt taaaacgtcg
    11101 tcccccgacc ttgctattgg acgtttgagc ggcggcaatc agcaaaaagc gatcctcgcc
    11161 cgctgtctgt tacttaaccc gcgcatcctc attcttgatg aacctaccag gggtattgat
    11221 attggcgcga aatatgagat ctacaaatta attaaccaac tcgtccagca gggtattgcc
    11281 gttattgtca tctcttccga attacctgaa gtgctcggcc ttagcgatcg tgtactggtg
    11341 atgcatgaag ggaaactaaa agccaacctg ataaatcata acctgactca ggagcaggtg
    11401 atggaagccg cattgaggag cgaacatcat gtcgaaaagc aatccgtctg aagtgaaatt
    11461 ggccgtaccg acatccggtg gcttttctgg gctgaaatca ctgaatttgc aggtcttcgt
    11521 gatgattgca gctatcatcg caatcatgct gttctttacc tggaccaccg atggtgccta
    11581 cttaagcgcc cgtaacgtct ccaacctgtt acgccagacc gcgattaccg gcatcctcgc
    11641 ggtaggaatg gtgttcgtca taatttctgc tgaaatcgac ctttccgtcg gctcaatgat
    11701 ggggctatta ggtggcgtcg cggcgatttg tgacgtctgg ttaggctggc ctttgccact
    11761 taccatcatt gtgacgttgg ttctgggact gcttctcggt gcctggaacg gatggtgggt
    11821 cgcgtaccgc aaagtccctt catttattgt caccctcgcg ggcatgttgg catttcgcgg
    11881 catactcatt ggcatcacca acggcacgac tgtatccccc accagcgccg cgatgtcaca
    11941 aattgggcaa agctatctcc ccgccagtac cggcttcatc attggcgcgc ttggcttaat
    12001 ggcttttgtt ggttggcaat ggcgcggaag aatgcgccgt caggctttgg gtttacagtc
    12061 tccggcctct accgcagtag tcggtcgcca agctttaacc gctatcatcg tattaggcgc
    12121 aatctggctg ttgaatgatt accgtggcgt tcccactcct gttctgctgc tgacgttgct
    12181 gttactcggc ggaatgttta tggcaacgcg gacggcattt ggacgacgca tttatgccat
    12241 cggcggcaat ctggaagcag cacgtctctc cgggattaac gttgaacgca ccaaacttgc
    12301 cgtgttcgcg attaacggat taatggtagc catcgccgga ttaatcctta gttctcgact
    12361 tggcgctggt tcaccttctg cgggaaatat cgccgaactg gacgcaattg cagcatgcgt
    12421 gattggcggc accagcctgg ctggcggtgt gggaagcgtt gccggagcag taatgggggc
    12481 atttatcatg gcttcactgg ataacggcat gagtatgatg gatgtaccga ccttctggca
    12541 gtatatcgtt aaaggtgcga ttctgttgct ggcagtatgg atggactccg caaccaaacg
    12601 ccgttcttga ttttgataaa aattttctca aagccggtta cgtattaccg gttttgagtt
    12661 tttgcatgat tcagcaggaa aagaaccatg tttactaaac gtcaccgcat cacattactg
    12721 ttcaatgcca ataaagccta tgaccggcag gtagtagaag gcgtagggga atatttacag
    12781 gcatcacaat cagaatggga tattttcatt gaagaagatt tccgcgcccg cattgataaa
    12841 atcaaggact ggttaggaga tgtcgtcatt gccgacttcg acgacaaaca gatcgagcaa
    12901 gcgctggctg atgtcgacgt ccccattgtt ggggttggcg gctcgtatca ccttgcagaa
    12961 agttacccac ccgttcatta cattgccacc gataactatg cgctggttga aagcgcattt
    13021 ttgcatttaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatat acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgaccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggcg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg gcaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga cgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacgcctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggcaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcgttga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgttgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtgcggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgctca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaaaca
    20341 aggaagccta ggatgaaagt gacatttgag cagttaaaag cagcctttaa tcgggtctta
    20401 atttcacgcg gcgttgacag cgaaacggct gacgcctgtg cagagatgtt cgcccgcacc
    20461 accgaatccg gcgtttattc tcacggcgtt aatcgtttcc ctcgtttcat tcaacaactg
    20521 gaaaacggcg atatcattcc tgatgcccaa cccaaacgta taaccagcct cggcgcaatt
    20581 gaacagtggg atgcccagcg ttcgatcggc aacctgacgg cgaaaaagat gatggatcgc
    20641 gccattgaac tggctgccga tcacggtatt ggtctggtgg cacttcgtaa tgccaaccac
    20701 tggatgcgcg gcggcagcta cggctggcag gcggcggaaa aaggctatat tggcatttgc
    20761 tggaccaact ccatcgccgt aatgccgccg tggggcgcaa aagagtgtcg catcggcact
    20821 aacccgctga tcgtcgccat tccttccacg ccgatcacca tggtcgatat gtcgatgtcg
    20881 atgttctctt acggcatgtt agaagttaac cgcctggcag gccgtcagct cccggtcgat
    20941 ggtggctttg atgatgaggg caatttgacc aaagaacctg gcgttatcga gaagaatcgc
    21001 cgcattttgc cgatgggcta ctggaaaggt tctggcatgt cgattgtgct ggatatgatc
    21061 gctactctcc tttccgacgg cgcatcggtt gccgaagtca cccaggacaa cagcgacgaa
    21121 tacggcattt cacaaatttt tattgccatt gaagtggaca agcttatcga cggtcccacc
    21181 cgcgatgcca agctgcaacg tatcatggat tacgttacta ccgctgaacg cgctgacgaa
    21241 aaccaggcca tccgcttacc cggccacgaa tttactaccc tgctggccga aaaccgccgt
    21301 aacggcatca ctgttgatga cagcgtgtgg gccaaaatcc aggcgttatg aggagataag
    21361 tcatgatttt tggacatatc gcgcagccca atccgtgccg tttgcccgcc gccattgaaa
    21421 aggcgctcga ttttctgcgc gctaccaact tcaacgtcct ggagccgggc gttgtcgaaa
    21481 tcgacggcaa gaatatttat gcgca
//
LOCUS       DAEEGA010000064        21388 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEEGA010000064 DAEEGA010000000
VERSION     DAEEGA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21388
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.064"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..135)
                     /locus_tag="KLH46_002688"
     CDS             complement(<1..135)
                     /locus_tag="KLH46_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0379315.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     I"
     gene            complement(237..1217)
                     /locus_tag="KLH46_002689"
     CDS             complement(237..1217)
                     /locus_tag="KLH46_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBE0379316.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1225..1935)
                     /locus_tag="KLH46_002690"
     CDS             complement(1225..1935)
                     /locus_tag="KLH46_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBE0379317.1"
                     /translation="MTENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2152..3975)
                     /gene="typA"
                     /locus_tag="KLH46_002691"
     CDS             complement(2152..3975)
                     /gene="typA"
                     /locus_tag="KLH46_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBE0379318.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4348..5757
                     /gene="glnA"
                     /locus_tag="KLH46_002692"
     CDS             4348..5757
                     /gene="glnA"
                     /locus_tag="KLH46_002692"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBE0379319.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5930..6979
                     /gene="glnL"
                     /locus_tag="KLH46_002693"
     CDS             5930..6979
                     /gene="glnL"
                     /locus_tag="KLH46_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBE0379320.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6991..8400
                     /gene="glnG"
                     /locus_tag="KLH46_002694"
     CDS             6991..8400
                     /gene="glnG"
                     /locus_tag="KLH46_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBE0379321.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8512..8622
                     /locus_tag="KLH46_002695"
     CDS             8512..8622
                     /locus_tag="KLH46_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBE0379322.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8851..10224)
                     /gene="hemN"
                     /locus_tag="KLH46_002696"
     CDS             complement(8851..10224)
                     /gene="hemN"
                     /locus_tag="KLH46_002696"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBE0379323.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VVRYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10413..10922)
                     /gene="yihI"
                     /locus_tag="KLH46_002697"
     CDS             complement(10413..10922)
                     /gene="yihI"
                     /locus_tag="KLH46_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBE0379324.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11504..12136
                     /locus_tag="KLH46_002698"
     CDS             11504..12136
                     /locus_tag="KLH46_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBE0379325.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12518..15304)
                     /gene="polA"
                     /locus_tag="KLH46_002699"
     CDS             complement(12518..15304)
                     /gene="polA"
                     /locus_tag="KLH46_002699"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBE0379326.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15668..16600
                     /locus_tag="KLH46_002700"
     CDS             15668..16600
                     /locus_tag="KLH46_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBE0379327.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(16641..18071)
                     /locus_tag="KLH46_002701"
     CDS             complement(16641..18071)
                     /locus_tag="KLH46_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBE0379328.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18226..18852)
                     /gene="dsbA"
                     /locus_tag="KLH46_002702"
     CDS             complement(18226..18852)
                     /gene="dsbA"
                     /locus_tag="KLH46_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBE0379329.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(18869..19855)
                     /gene="srkA"
                     /locus_tag="KLH46_002703"
     CDS             complement(18869..19855)
                     /gene="srkA"
                     /locus_tag="KLH46_002703"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBE0379330.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19932..20201)
                     /locus_tag="KLH46_002704"
     CDS             complement(19932..20201)
                     /locus_tag="KLH46_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBE0379331.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20271..20855
                     /gene="mobA"
                     /locus_tag="KLH46_002705"
     CDS             20271..20855
                     /gene="mobA"
                     /locus_tag="KLH46_002705"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBE0379332.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20837..21364
                     /gene="mobB"
                     /locus_tag="KLH46_002706"
     CDS             20837..21364
                     /gene="mobB"
                     /locus_tag="KLH46_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBE0379333.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5234 a         5364 c         5143 g         5647 t
ORIGIN      
        1 aatttgacca ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag
       61 cgacggtaat ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt
      121 ctttttcgtg agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata
      181 agataccgac cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag
      241 tggttcagaa gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt
      301 atctgattaa ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct
      361 ggtaggggtg tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca
      421 aagcggaacg ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc
      481 ggctcatcat cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc
      541 tgacctgaaa tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct
      601 ttaatatgga cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca
      661 tttgcattga tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct
      721 ttgaccagcg acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc
      781 aggccgctgt cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat
      841 acgtcgcgca aattaccttc ataacgtgga taaaaacgta cggacgttgc cccagttttc
      901 aacgcgatgg cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga
      961 atatcaaggt tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata
     1021 ttaccaagag aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt
     1081 tcacttgcga tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga
     1141 aaggcataag catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg
     1201 ttatttattg tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg
     1261 ccaatattca atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat
     1321 taagatcgct ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct
     1381 gacgggtttg aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac
     1441 tgcagaaaat gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat
     1501 atatgtttcg tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata
     1561 gagttcagtt gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt
     1621 acggtttttc tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg
     1681 aaagctcgcc gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt
     1741 acgatcctta cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt
     1801 catacgttta atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg
     1861 cgtggtgatg ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga
     1921 ttgattctcc gtcattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta
     1981 cgccaaaaaa gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc
     2041 tattgacgta atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg
     2101 taaaaaaaaa cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc
     2161 tttcggtgcg cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg
     2221 gatagaggtc ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc
     2281 cagagtcatg cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat
     2341 gttggtcagt ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat
     2401 accgataatc tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc
     2461 ctgcagaccg aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc
     2521 gttctgacgc tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta
     2581 cagcagacca gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg
     2641 gcttgggatc acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag
     2701 gtcgccttta cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc
     2761 cagcgtcacg ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt
     2821 cggacgggat accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag
     2881 gtgcagttca ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg
     2941 cagcgcaacg ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac
     3001 gaacttacct tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt
     3061 cggctcatca acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc
     3121 agaaatgttc agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc
     3181 ggtttcgata cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt
     3241 tttgccttcg ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat
     3301 gcggccaatg ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg
     3361 gaacggaccg tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag
     3421 cggggtcatg tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga
     3481 agcgtaaacg atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc
     3541 gaatacctga tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac
     3601 aacaatcggc ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat
     3661 cgggccgtca aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc
     3721 aacttcacca ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt
     3781 ccatttgata gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt
     3841 ggagtccatc acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag
     3901 cagcttgtct accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg
     3961 caatttttcg atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat
     4021 taatcgcact acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct
     4081 ttgtgatcgc tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt
     4141 gcttaatgtt tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt
     4201 ttgcacgatg ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt
     4261 cgctttatct tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga
     4321 caatgaccaa tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac
     4381 gagcacgaag tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac
     4441 gtcactatcc ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac
     4501 ggctcctcga ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac
     4561 gcatccactg cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac
     4621 atccttgaac ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc
     4681 gccgaagatt acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct
     4741 gaattcttcc tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct
     4801 atcgacgata tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt
     4861 caccgtccgg cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat
     4921 attcgttctg aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac
     4981 cacgaagtag cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa
     5041 aaagctgacg aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt
     5101 aaaaccgcga cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc
     5161 cacatgtctc tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg
     5221 tctgagcagg cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc
     5281 ctggcaaacc cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta
     5341 atgctggctt actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct
     5401 ccgaaagcac gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc
     5461 tttgctgccc tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa
     5521 gccatggaca aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt
     5581 gcaggctctc tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc
     5641 ggtggcgtgt tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat
     5701 gaccgcgtgc gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag
     5761 ttgaagttgt actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc
     5821 catctctgca tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta
     5881 aaaagctata atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg
     5941 cacgcagccc gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga
     6001 tgacaatctg gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc
     6061 ccgcaaattg tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga
     6121 gctgatgcaa gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt
     6181 catcgatggt cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat
     6241 cctgctggag atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca
     6301 tgctcaacaa gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc
     6361 gcttggcggt ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact
     6421 actcgaatat accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg
     6481 tctgttgggg ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga
     6541 acgcgtggta acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta
     6601 cgatcccagc ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa
     6661 tattgtgcgc aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac
     6721 ccgcaccgcg tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga
     6781 tgtggaagat aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat
     6841 ggtcagcggc cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga
     6901 tcagcattca ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta
     6961 cctgcctatc aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat
     7021 gacgatagtt ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt
     7081 acgacatttg agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg
     7141 ctgctttcag atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa
     7201 cagcgccatc caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc
     7261 gtcagcgcct atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa
     7321 gcagtggcgc tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat
     7381 attcagctta acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg
     7441 ttccgtatta tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc
     7501 ggcaccggta aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg
     7561 ccgtttatcg cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt
     7621 ggccacgaga aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag
     7681 gccgatggcg gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg
     7741 cgtttgctgc gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg
     7801 aaagtggatg tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa
     7861 ggtaagttcc gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg
     7921 ctgcgcgaac gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg
     7981 cgcgaactgg gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt
     8041 ctggcgtggc caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg
     8101 gccgccgggc aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt
     8161 gcggagagta cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca
     8221 gacagagcgc tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag
     8281 cggacgttac tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg
     8341 ctactcggct ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga
     8401 ttcacggctt gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta
     8461 ctgttccgat aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa
     8521 tcaataatta atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt
     8581 gctgccgtgc tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg
     8641 gatgcggaga aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct
     8701 cagatgccag atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg
     8761 ttttgttgcc cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt
     8821 ctactttgta aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg
     8881 cgctttctgg cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc
     8941 tttcgccgtc acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg
     9001 ggcgagcagc ttgagatctt cctcaaagta atcagcgaag tgcaaatccc actgtttctc
     9061 aagaggggcg taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat
     9121 acagtcatca cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg
     9181 ctgatagtac tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat
     9241 ggcggaaacg cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt
     9301 acgatgcagc acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg
     9361 atccataccg ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat
     9421 atcgagtttt tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa
     9481 aatggtcggc agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac
     9541 acgtttcagg gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc
     9601 gatgttggtg gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc
     9661 ttcatcctgc tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat
     9721 gctcaggcga ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg
     9781 cggatcgact tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag
     9841 cttcatcaga cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg
     9901 caattggctg acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag
     9961 cgcgtccaga tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca
    10021 gaagtaacaa agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg
    10081 atagcgcacc acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc
    10141 ggtcgggtac gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc
    10201 ccagtcgatt tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg
    10261 cgtaccggcg gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc
    10321 gacaaccgct gtagtttaac gaataaccac accagataac atataaccgg aagggttatc
    10381 agcagaacgg gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg
    10441 tcttcttgct tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc
    10501 tgcatcaact catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc
    10561 agggtttcgc ctgcttccag acgttccagc agcgcatcca gacgctcatc cgtttccagt
    10621 aattccaact ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg
    10681 gtgacttttt cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt
    10741 ggtgcgttct ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc
    10801 gcatgaccac gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg
    10861 cgtgtttttc gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc
    10921 ataatggttc gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa
    10981 agcaagcaaa gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc
    11041 ttaacgggtc ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa
    11101 caatccgtgt tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa
    11161 tcctctgtct tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc
    11221 gtcctcgctc tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc
    11281 agtatttgca cattttcaga gttttatata aaagcaattc actggtttat atagcttatt
    11341 tgacatgcat cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac
    11401 aaagcgtatg gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc
    11461 aggtataatc gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta
    11521 tcaacagacg cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg
    11581 aattgaagtg gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct
    11641 gactaaccag aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa
    11701 cctgtttgaa gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga
    11761 agtcccggaa gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg
    11821 tcagagcctg caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga
    11881 tcagcagatg attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa
    11941 agcggacaaa ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt
    12001 actggcgttt aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt
    12061 agacaagctg aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac
    12121 gcaggacggc gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc
    12181 gtaaattcaa tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta
    12241 cggatctttt ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa
    12301 atattcagcc aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc
    12361 tgtaccctac gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat
    12421 tttttttcag ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt
    12481 tgcttactta cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt
    12541 cgccactccc cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt
    12601 gatgaatctg cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat
    12661 cgtgtacctg catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg
    12721 caatcatcgc ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg
    12781 cacgttcagc cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac
    12841 ggcgtccgtc cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca
    12901 tatactccag cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt
    12961 tacgtggaat gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa
    13021 agttgatcgc tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa
    13081 acacttctgc cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc
    13141 ctttatcacg cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga
    13201 caatcacata atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac
    13261 gcaccggaat gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg
    13321 cctggtgata agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt
    13381 cggtgtaagt cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg
    13441 gatagtccag cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg
    13501 ttttcttcag cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg
    13561 aagaaaggtt aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac
    13621 gcaaggtgag ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac
    13681 gttcaatgcg tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg
    13741 gccctttgtg tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg
    13801 catcttcggc ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat
    13861 ttttgccttt acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg
    13921 cgaggctgtc catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg
    13981 tatcaaacgc aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt
    14041 tcaggttttg cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga
    14101 gtgcacgctc gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat
    14161 atgccgctac gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat
    14221 caaggctgtc ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc
    14281 acgctttcag tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg
    14341 ccgtcacttc tggcgcttcg tctgcaacac tggtttcctg cggcttcgcg gctgtttttg
    14401 cccctttggc ctgtaaccat ttgcccgctt cgacatcagc agtccagcgt ttgaactcat
    14461 actttttgaa cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac
    14521 aggtcagctc cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa
    14581 cttctttgtt ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag
    14641 caattttttc tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct
    14701 gcgcggtttt ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca
    14761 gcgccaggaa gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg
    14821 gtccgaggat ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg
    14881 ccatatcttt atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg
    14941 ccagagtacc gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca
    15001 tcgctttaac catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg
    15061 ggcgatgtga tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa
    15121 agaccaccgc tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga
    15181 caccatacat cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat
    15241 atgcgcgata aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa
    15301 ccataatgtc cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa
    15361 acatgagttt ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa
    15421 atcgtcatgt ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg
    15481 ctttttacag catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta
    15541 ataattgaga taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa
    15601 ggatcatccg ttatttgggt cattttctgc taaaatctgc gccttgcgct tctttagtgc
    15661 actgtctatg gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac
    15721 ccttttgctg agtatcgtat tgaccatttt ggtcactatt ttttgttccg tcccgatcat
    15781 cattgccggg attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg
    15841 tttttgtgat tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa
    15901 cccacacctg caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct
    15961 gcttatttgt aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa
    16021 gcacattccg atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg
    16081 cctggcgtgc tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt
    16141 acgtcatccg gagcgacgtg gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt
    16201 tcgcctgcat cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa
    16261 acatcagcaa acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc
    16321 gatggcgcta aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta
    16381 tccggacaat aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt
    16441 cgtacatgtg gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa
    16501 aagctttaag cgccattttc agcaatggct gaactcattg tggcaggaaa aagaccggct
    16561 tctgacgtct ctcatgtcat cacagcgtca gaataagtaa gttttactcg cctctgaatg
    16621 agcagaggcg agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct
    16681 tcgctcatct cctgtccgtt aaaaacaact ttacccggtg tataacgcag ctgcagcgag
    16741 gcggtattgt tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg
    16801 atttgtttat cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc
    16861 tgttttgcgg tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg
    16921 atatctttgt ttgtggatga tgaagatttg gctgggtcgg caatactgat atccagattg
    16981 gcatttagtt cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt
    17041 ttttgtaata acggtaaata ctctttgaac aatgcagcat tcacttcttg cagagcaact
    17101 tcatcgtttg ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg
    17161 atgataaatt ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca
    17221 ctaccgagtt gctgattccc ttttgttaac ttatcaagtt cgtaggtcag attgacattg
    17281 atataatctt tgtctttaac gcgatccagc ctggtttttg caacgaaagc atcgatttgg
    17341 gcaacgtctt ccccgtgatt gataatgtta atctgattaa atttcgactc gctttcacca
    17401 accggaaatt tcttttcttc taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt
    17461 actttgttca ttggtgataa ttgcagaaca atatcttcga ctttgccttc aatatccagg
    17521 ttagacaatg aactaccatc accattaaaa gtaaactggc cctcaccaaa agaaaatttt
    17581 ccatactctg caggggcaac atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc
    17641 aatgtcgctt ccacgggcga tttatttttc gcggcgataa atagcggttg tgtcagttca
    17701 ttattcacta agttcacttt tgccgctgcc agtgctggga tgacattacc atgcattaac
    17761 attgtgatgg gcaacggacc gtgctcaaca tccacatcaa atacaacttt ctggcctggc
    17821 ttgatattga gatcgggtgc accgttatcg aatgtcattt gcatttgaaa acgggaatta
    17881 aaaaagcctt tgtcaaagtt tttataactt aacgtcatat cgtaggcatg ttcacctttc
    17941 tttttcgcat cgttaaaatc tttaataaac ttttcgatac caggctgaat ttgcgtacct
    18001 gtgtaccatg tgccaccacc ccagattacg gctaacgcaa caataacacc tgtagctgac
    18061 ttacgtatca tataaatatt ccctgataaa acagcagaac tttgaatatc cacttatgct
    18121 gaaataatgt ccagcggcag gatgcattat cagaatgtat ttatatttat taaataataa
    18181 aaaaagcccg tgaatattca cgggctttat gtaatttaca ttgaattatt tttcctcgga
    18241 cagatatttc accgtatcag catactgctg aacaaaaaca tccatattgc tggtatccat
    18301 accctgcgga ttcagctgat atttaccgtt aacaaacatc gccggaacac cacgcaattg
    18361 cacatcagct gcggcttttt cctgctgagc gaccagagat ttcaccacga agctgttcca
    18421 cgccgcgtcg tactcttcac ctttaatacc tgcattgata aatacatcgc ggatatcaga
    18481 tgctgaacga atggtctggg ttttctgtac gccttcaaac agcggaacag tcactttgtc
    18541 ttccacgccc agcgccatcg ccacagccca tgcctgagtc agatctttgc ccaggtcacc
    18601 acccatgaag ttgacgtggt atttagtcat cttcacgcct tccggcagtt ttttcttcac
    18661 attatcagaa atatgcagaa cttcttcaaa ctgatagcag tgcgggcaga agaaagagaa
    18721 aaactccagc acttgcggcg cgccagctac cggtttttcc agggtagtgt actgtttacc
    18781 atcttcatac tgcgccgccg atgcgctaaa cgctaaaact aaaccagcca gcgccagcca
    18841 aatctttttc atgatctact ctctccgatt aatacatagg tgttaattgc aaagggggtt
    18901 cttgtagaac ttttgcctgt tctataaaag tcgccgtctg tcgtagccag taatcttccc
    18961 cggttaacca cgggaaattt ttcgggaacg cgggatcagc ccaatgccgc attagccagg
    19021 caagataata aaccaaacgc atggcgcgta aaggttcaat cagtccgatt tcagcggtgt
    19081 cgaactcgct aaattcttca taagcttcaa taatagtttc cagttgcatc cgctgctggg
    19141 ctttatcgcc attgagcaac atccacaaat cctgaatagc tggaccatta cgtgcatcat
    19201 ccagatcaac aaacattgga ccatcgcgcc agagaatatt cccggcgtgg cagtctccat
    19261 gtagccgcag aacggtgaaa tcttcccgcc agtgtgctgt aacggcggcg atcagctcat
    19321 ctgtcgcttt cagaaatgcc gctttcaacc cggaaggtat cagtgtagcg tcctcaaaca
    19381 gcttgcgagg ctcaatgaga tattcattca gaccgatggt cgggcgatga ataaaaagct
    19441 gtttgcgccc cgtctggtgc atacgcccta aataacgccc aaccgcttcc atctgatcga
    19501 tattatcagc ttcgaactgg cgaccaccga cgcttggaaa aacagcgaaa taaaatccct
    19561 gatgattcaa taaagtctga ccgttaaagg ccacaggtgc tgcgaccgga acttcatcat
    19621 ttaccagctg caacgcaaat tgatgttctt cgaggatttg atcggctgtc caacgttcag
    19681 ggcgataaaa tttgacgaca aaacgtcgac gctcttcgtc ctgaaattga tagacacggt
    19741 tttcatagct gttaagcggg gtaagaccgg aatccacccg gatcccttgc tcaaacagag
    19801 cgtccatgat ggtatccggg tgtagtgtct ggaaagtaaa agcgctgttg ttcatcccat
    19861 catccggaaa acattacgaa tgatgaagga tatcatttca cgatgatttt ggtggcgccg
    19921 cttacaagct tttactcttt aattacgccg cgagcgcgta acagcgccgt tttgaagtct
    19981 tcttcataat ctttctgcaa acccgggatc accgcatctt tcgcggaatc acgcattttc
    20041 aggtgataga tcagaatgtc atccgtcaaa tccgccagtt cgccgtcaaa acctgactct
    20101 ttcgccagtt tctgcaaaaa ttgcaacaag ttaaagtctg gctctttttg ccaggctggc
    20161 tggaggagtt caataacttc attcagacgt ttacatttca tagtaatgct ccttactctt
    20221 gagacagaca cgttagcagg gtcaatccca caataaaaga ggcgatatcg gtgaatctga
    20281 tgacgacgat aacaggcgtt gtgctggcag gcggtaaagc cagacgaatg ggcggcgtag
    20341 ataaaggatt gctggactta aatggcaaac cgctgtggca acatgtcgct gacgcgctta
    20401 agacgcagct ctctcacgtc gtggttaatg ctaatcgtca tcaggaaatc tatcaggcag
    20461 gcggtctgaa agtgattgaa gattcactgg cggattaccc aggccctctg gcaggaatgc
    20521 tttcagtaat gcagcaggaa gctggtgagt ggtttttgtt ttgtccgtgc gatacgcctt
    20581 acattcccca tgatttagcc gcccggctta atcatcagcg caaagatgcg cctgtcgtgt
    20641 gggtccatga cggtgaacgc gatcacccga ctattgctct ggtaaaccgc gctattgagc
    20701 ctttattact ggaatatctg caagcaggag aacgccgggt aatggcattt atgcgtctgg
    20761 ctggcggtca tgcggttgat ttcagcgatc ataaagatgc atttgttaac gtgaatacgc
    20821 cagaggagct tgcccgatgg caggaaaaac gatgataccg ttactcgcct ttgccgcgtg
    20881 gagtggtacc ggaaaaacta cgcttttgaa aaaactgatc ccggcattat gcgccagagg
    20941 gatccgtccg gggctgatta agcatacgca ccatgatatg gatgttgata agccaggcaa
    21001 agatagctat gagctgcgca aggctggcgc ggcacaaacc atcgttgcca gccagcaacg
    21061 atgggccttg atgacggaaa caccagacga agaagagctg gatctacatt ttctcgcaag
    21121 tcgaatggat acctcaaagc tggatttgat tctggtcgaa gggtttaagc atgaagagat
    21181 cgcaaagatt gtgctgtttc gcgatggagc cggacatcga cctgaagaat tagtgataga
    21241 caggcatgtt attgctgtag ccagtgatgt gccgcttaat cttgatgtcg cattactgga
    21301 tattaatgat gttgaggggc tggctgattt tgtggtggag tggatgcaaa agcagaacgg
    21361 ataagcaaga gtaggctgga tcagacgc
//
LOCUS       DAEEGA010000065        21277 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEEGA010000065 DAEEGA010000000
VERSION     DAEEGA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21277
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.065"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..446)
                     /locus_tag="KLH46_002707"
     CDS             complement(<1..446)
                     /locus_tag="KLH46_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBE0379334.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /gene="manZ"
                     /locus_tag="KLH46_002708"
     CDS             complement(501..1352)
                     /gene="manZ"
                     /locus_tag="KLH46_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBE0379335.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /gene="manY"
                     /locus_tag="KLH46_002709"
     CDS             complement(1365..2165)
                     /gene="manY"
                     /locus_tag="KLH46_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBE0379336.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="KLH46_002710"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="KLH46_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBE0379337.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMVPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="KLH46_002711"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="KLH46_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBE0379338.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..6814)
                     /locus_tag="KLH46_002712"
     CDS             complement(5216..6814)
                     /locus_tag="KLH46_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBE0379339.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="KLH46_002713"
     CDS             complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="KLH46_002713"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBE0379340.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8493..9071)
                     /locus_tag="KLH46_002714"
     CDS             complement(8493..9071)
                     /locus_tag="KLH46_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBE0379341.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="KLH46_002715"
     CDS             complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="KLH46_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBE0379342.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10510..10689
                     /locus_tag="KLH46_002716"
     CDS             10510..10689
                     /locus_tag="KLH46_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBE0379343.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10809..11168)
                     /locus_tag="KLH46_002717"
     CDS             complement(10809..11168)
                     /locus_tag="KLH46_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBE0379344.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11530..11874)
                     /locus_tag="KLH46_002718"
     CDS             complement(11530..11874)
                     /locus_tag="KLH46_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBE0379345.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12006..13916
                     /locus_tag="KLH46_002719"
     CDS             12006..13916
                     /locus_tag="KLH46_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBE0379346.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13974..14669
                     /gene="tsaB"
                     /locus_tag="KLH46_002720"
     CDS             13974..14669
                     /gene="tsaB"
                     /locus_tag="KLH46_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBE0379347.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14709..15290
                     /gene="yeaY"
                     /locus_tag="KLH46_002721"
     CDS             14709..15290
                     /gene="yeaY"
                     /locus_tag="KLH46_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBE0379348.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15495..17180
                     /gene="fadD"
                     /locus_tag="KLH46_002722"
     CDS             15495..17180
                     /gene="fadD"
                     /locus_tag="KLH46_002722"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBE0379349.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHALEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLGQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17262..18377
                     /gene="rnd"
                     /locus_tag="KLH46_002723"
     CDS             17262..18377
                     /gene="rnd"
                     /locus_tag="KLH46_002723"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBE0379350.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18431..19396)
                     /gene="yeaX"
                     /locus_tag="KLH46_002724"
     CDS             complement(18431..19396)
                     /gene="yeaX"
                     /locus_tag="KLH46_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBE0379351.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGIPPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19452..20069)
                     /gene="yeaW"
                     /locus_tag="KLH46_002725"
                     /pseudo
     CDS             complement(19452..20069)
                     /gene="yeaW"
                     /locus_tag="KLH46_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20411..20977)
                     /locus_tag="KLH46_002726"
     CDS             complement(20411..20977)
                     /locus_tag="KLH46_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0379352.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21189..>21277
                     /locus_tag="KLH46_002727"
     CDS             21189..>21277
                     /locus_tag="KLH46_002727"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0379353.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5268 a         5274 c         5467 g         5268 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacaacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcacc cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggca ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggctgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttctccttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt attgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcctt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca
     5401 ccagttcaat attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa
     5461 ccgcgtcaga acctatagct gcggtaaatg acttatcaat tttcagcacg tcaggacgta
     5521 atgtttcaag ccaggaaaac gagctgttgc cggtgccgaa gtcatcaatc gccagtttga
     5581 cgtttttacg atgcagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt
     5641 cggtgatttc gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat
     5701 cttttatcag cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact
     5761 gactactcat cgggaaaaca tggtgctgac gaatggtttc tgccatcaca tagtgggtca
     5821 gtggcacaat taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc
     5881 cctgacgcgg attattccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg
     5941 cattaagcaa aggctgacag aacaattcga actcatgttg cgccaggccc agattaattt
     6001 cgcgggaaaa gctcatccgg taagcggtcg ccagccaggc gatatagccc accagtaaac
     6061 ttagcagcac cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc
     6121 ccggaccatt tacgttaatg gtaaaaggaa agctttgcga agaaacctga tagttttcat
     6181 tgtcttcagg ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt
     6241 ccaccgtcag gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca
     6301 gtaagtcgat gttaatcatc tctatgaccc cagcattgct actgcccgct gcaggcgtcc
     6361 attgaatcaa aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg
     6421 gttgcggtgc aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa
     6481 tgctggagca ataaagtgtg ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt
     6541 ttgccgcctg tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa
     6601 gcaagacttt gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt
     6661 gattggcgaa ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc
     6721 tcagggtcga agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc
     6781 gataggtttt aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg
     6841 acaaacgcct gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg
     6901 ggtataaatt cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact
     6961 ttgattgcca gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccggtt
     7021 tcgtacatcg tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga
     7081 gccatccgtg cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc
     7141 acctgaacct gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt
     7201 tccgccgcca cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca
     7261 gccattgaac aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag
     7321 gcgttcattt tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg
     7381 tctgggctga ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg
     7441 caggcaccgt tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact
     7501 gccagcgcaa acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg
     7561 ctggaaacta acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc
     7621 agtacacctt cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc
     7681 gcgaaatact cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga
     7741 ccagacagcg aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac
     7801 ggatacggca cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata
     7861 aaaccgccgc cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg
     7921 tggatttgca taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg
     7981 tcgcgcggga aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctctttt
     8041 acgtcgcgaa taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca
     8101 agacccataa taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca
     8161 taaacgtcca cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg
     8221 acgaactgtt tacccgcctt cataggccct acggtatggg aagatgaggg accaatcccc
     8281 accttaaaca tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc
     8341 cagtaacgaa ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat
     8401 tcacatgaat taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa
     8461 gtagtgtttt gtagatgtaa agataagtat agtcaggggt tcacaccaat ttgcagcgcc
     8521 agctcacgaa ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggacagc
     8581 catacccgat gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt
     8641 aatgcctggg cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac
     8701 ggcagatcgg gcgggataat gccgaccact ggggttactt gatagccagt tacgctatcg
     8761 acgggcggca gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca
     8821 gcttcgcgca gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg
     8881 aatgccactt gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc
     8941 ggttgcggtc gacggacgat ggggattaac acggcagcct gacgatgatt taggggttcc
     9001 cggttaattt gcgggcgcaa aagttgaaag cgagataaaa aatcatcaag cgtcaggcta
     9061 cggtattcca cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt
     9121 tcctgatatt ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat
     9181 atttgtccgt taatggcagt cagcgtgcgg atagtaatgc tggtatccat gttgccgcaa
     9241 aagctcaaat agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg
     9301 ataatttcca tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg
     9361 cgcagcagat cgctggcgtg taactgttct ggtagtcgcg ccgttatagt gctgaccaga
     9421 tgatgcacgg cagggaaggg ttccaccacg aagagctctg gtacttttac cgaaccggct
     9481 acggcaacac gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga
     9541 tctttcgctg agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg
     9601 cgtggtagcg tgcctttaat cgggcgggtc tggatttcac tattatcaca aagaataaac
     9661 cgctctggcg aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc
     9721 gcgcggttgg cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca
     9781 tgaaaacgct gggcgagatt cacctgatag caatcaccgc tgtgcagata ttcctgtacc
     9841 tggcgaaatt tttcgccgta ctgctcgtgg gtcatattgg attgccagtc ggaagtgagc
     9901 gtgaaatctt cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca
     9961 tcattatgac tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg
    10021 tagataccca ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac
    10081 tcaaaacggc ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa
    10141 tcttcgttat gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc
    10201 gggtcatcag tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa
    10261 gtggttaaag tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg
    10321 gcatagccgg agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat
    10381 tcagcggcgt cctgacgcca gggtaaagta atcacagtgg gagataacgt cttcatcagt
    10441 cctgactcta ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag
    10501 gagttaacaa tgtttgcagg tttaccttca ctcatccatg aacagcagca aaaagctgtc
    10561 gagcggatcc aggaactgat ggcacaaggg atgagtagcg gccaggcaat cgcgctggtg
    10621 gcagaagagc tgcgtgccaa ccactccggc gagctaatcg tggcgcgttt tgaggatgaa
    10681 gacgagtaac acactaatcc gtatcgattt cgttatagaa gccgatgatc atttgattgt
    10741 gagaaaaagg acgcttttta tatgtgatta aaagcgtcct gacccattta tgctgatata
    10801 aatttaactc agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga
    10861 tttttaatca cagagcgttc acctgtctcc attttttttg cccatccaac cattttttcc
    10921 gtgaacccag ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgcg
    10981 ggtactccca gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa
    11041 cttggtgttg gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca
    11101 gcaggcatat tcttatcctt aaaattaacc atgttatcga taaggctata tatgacctta
    11161 atttgcatct aatatcaaag ggattataac gatatggcac gagttaacct gttgacattc
    11221 aggtgtttta aaggtagcag ctcatggtgt gttatccagt aagatggaga aaattaacgg
    11281 tcagcccgca cgctataacc agattgttca gctaagaaag aaaaccagag ttgctaatca
    11341 aagggcgaac acaacggcat ggttatgttt atttatatag ttagttttat agataaaatt
    11401 gaaattataa atataaataa tgaattagca gaagcatcaa cagtaatatc ttattctctg
    11461 gtcgatttat gtaaaaccga ccagtattat cgataaatta tcgtctgact tattttgctt
    11521 cgataaagct tacaccgcag ccacaatctt aatttcaact ttatacttcg ggttcatcaa
    11581 acccgcttgt accgtgcagc gcaccggcgc atgacccgcg acaacccagg catcccacgc
    11641 tttattcatc gccgcgaagt cgtttttatc ggccaggaaa atggtggcat ccagaatgct
    11701 cgatttattg ctgccctgtt tttccagcac ggcgtcaatc tgtgccagcg tgttggcggt
    11761 ttgctcaaag gcatcggcgt cgaggttttc cggtacacca gtgtagtaga gcgtgttgtt
    11821 gtggattact acatcagacc agcgggcttc agcatcgata cgaacgatag tcatcatttt
    11881 gtcctcatta tacttccatt ttcgtgctgc aaagactgcc acaatgtgag gttgccgtca
    11941 ccacttcaac tggcgaaagc gccctcatcc tgacataatc cctgttcaaa tcaacagggg
    12001 gtagtgtgac ggacgatttt gcaccagacg gtcagctggc gaaagcgata ccaggcttta
    12061 agccgcgaga accacagcga cagatggcgg tagccgtcac ccaggcgata gaaaaaggcc
    12121 agccgctggt ggtggaagca ggaaccggta cgggcaaaac ctacgcttac ctggctcctg
    12181 cgctgcgggc gaaaaagaaa gtcattatct cgaccggctc aaaagcgttg caggatcagc
    12241 tctacagccg cgatttgcca acagtctcaa aggcattgaa atatacgggc aacgtggcgc
    12301 tgctgaaagg gcgctcaaac tacctctgcc tcgaacgtct cgaacagcag gcgctggcgg
    12361 ggggcgatct gccggtacaa atcttaagcg atgtgatcct gctgcgctcc tggtctaatc
    12421 aaacagtcga tggtgatatc agcacctgcg tcagcgtggc ggaagattca caggcgtggc
    12481 cgctggtcac cagcaccaac gacaactgtc ttggcagcga ctgcccgatg tataaagatt
    12541 gctttgtggt caaagcacgt aaaaaagcga tggacgccga tgtggtggtg gtaaaccatc
    12601 atctctttct ggcggatatg gtggttaaag agagtggatt tggcgagttg atccctgaag
    12661 cggacgtcat gatcttcgac gaagcccacc agctaccgga cattgccagc cagtattttg
    12721 gtcagtcact ctccagtcga caactgctcg acctggcaaa agacatcacc atcgcctacc
    12781 gcaccgaatt aaaagacacc cagcagttac aaaagtgcgc tgatcgtctt gcccagagtg
    12841 cgcaggattt tcgtctgcaa ctcggtgagc caggttatcg cggtaacctg cgtgagctgt
    12901 tagctaatcc gcaaattcag cgggcatttt tactgctcga tgacaccttg gaactttgtt
    12961 atgatgtggc gaaactgtca ctggggcgtt ccgccttgct ggatgcggca tttgagcgcg
    13021 ccacgttgta tcgcacacgg ctgaagcggc taaaagagat caatcagccg ggctacagct
    13081 actggtacga atgcacttcg cgccatttta ctctggctct cacgccgctc agcgtggcgg
    13141 ataaattcaa agagttaatg gcgcaaaagc ccggtagctg gatcttcacc tcagcaacgc
    13201 tgtcggtgaa cgacgatctg catcatttca cctcgcggct tgggatagaa caggcggagt
    13261 cgttgctatt gccaagcccg tttgattaca gccgccaggc gttactctgt gtgccgcgca
    13321 atctgccgca aaccaaccag ccaggttctg ctcgccagtt agcggcaatg ctgcgaccga
    13381 tcatcgaagc taacaacggt cgttgtttta tgctttgtac ctcgcacgcc atgatgcgcg
    13441 atctggccga gcagttccgc gctaccatga cgcttcccgt tttgttgcag ggggaaacca
    13501 gtaaaggcca actgttgcag cagtttgtca gtgccggtaa tgcgcttctt gtggcaacca
    13561 gtagtttctg ggaaggggtg gacgtgcgtg gcgatacatt gtcattggta attatcgaca
    13621 aattgccgtt tacctcgccg gatgatccac tgttaaaagc gcgcatggaa gattgtcgtt
    13681 tgcgcggtgg cgacccgttc gatgaagtgc aactaccaga tgccgtcatt actctcaaac
    13741 agggggtagg tcgactgatt cgcgacgccg acgatcgtgg cgtgctggtg atttgtgact
    13801 atcggctggt gatgcgtcct tacggcgcga cgtttctcgc cagtctgccg cccgcgccac
    13861 gcacccgtga cattgcccgt gcggttcgtt tccttgcgat accatcctcc aggtaatttg
    13921 ttacggagta tggtatgatg cgcgcctttt tattgatcta agcacgaaga cccatgcgaa
    13981 ttctggctat cgataccgcg acagaggcct gctctgtcgc cctgtggaac gacggtactg
    14041 tcaacgctca ttttgagctt tgccctcgtg aacatactca acgaatctta ccgatggtgc
    14101 aggatatcct gaccaccagc ggaacttccc tgactgatat taacgctctg gcttacgggc
    14161 gcggccccgg tagctttacc ggcgtgcgca ttggtattgg catcgcgcaa gggctggcgc
    14221 ttggcgcgga attaccgatg attggcgtct ccacgctaat gacgatggcg caaggggcgt
    14281 ggcgcaaaaa cggcgcaaca cgcgtgctgt cagccattga cgcgcgaatg ggcgaagttt
    14341 actgggccga atatcagcgt gatgaaaacg gtatctggca cggtgaagaa accgaagccg
    14401 tactcaaacc cgaactcgtt catgaacgaa tgcaacagct ttccggcgaa tgggtgacgg
    14461 tagggacggg ctggcaagcc tggccggatc tcggtaaaga gagcgggctg gttttgcgcg
    14521 atggcgaagt gttactcccc gctgcagaag atatgctgcc gattgcttgt cagatgtttg
    14581 ccgagggtaa aacggtggcg gtggaacatg ccgagccggt ttatttgcgt aacaacgtcg
    14641 catggaagaa acttccgggc aaagaatgaa tcttaagagt atgccctgag aaaaaaagga
    14701 gtcgcatcat ggcggttcaa aagaatgtta tcaaaggcat actggcaggt acgtttgcgc
    14761 taatgctgag cggttgtgtc actgtgccgg acgccattaa aggcagcagt cccacgccgc
    14821 aacaagattt agttcgggtg atgagtgcgc cgcagctgta cgttggtcag gaggcacgct
    14881 ttggtggcaa agtggttgcg gtacaaaacc agcaagggaa aacccgcctg gaaattgcta
    14941 ccgtaccgct ggacagcgga gctaggccga cgctgggaga accttctcgc ggtcgcattt
    15001 atgccgatgt gaacggtttt ctggacccgg tggatttccg tggacaactg gttacggtag
    15061 tcgggccaat caccggtgcg gttgacggca aaatcggcaa tacgccctat aaatttatgg
    15121 tgatgcaagt aacgggttac aaacgttggc atttaaccca gcaggtgatt atgccgcctc
    15181 agccgattga tccatggttt tatggcggtc gtggctggcc ctatggctac ggcggatggg
    15241 gctggtataa tcccggcccc gcgagagtac aaacagttgt aaccgaataa ttgcttgttt
    15301 ttaaagaaaa agaaacagcg gctggtccgc tgtttctgca ttcttacggt aaagataaaa
    15361 ataaatagtg acgcgcttcg caaccttttc gttgggtaat tatcaagctg gtatgatgag
    15421 ttaatattat gttaacagcg tgtatatcat ttggggttgc gatgacgacg aacacgcatt
    15481 ttagaggtga agaattgaag aaggtttggc ttaaccgtta tcccgcggac gttccgacgg
    15541 agatcaaccc tgaccgttat caatctctgg tagatatgtt tgagcagtcg gtcgcgcgct
    15601 acgccgatca gcctgcgttt gtgaatatgg gggaggtgat gaccttccgc aagctggaag
    15661 aacgcagtcg cgcgtttgcc gcttatttgc aacaagggtt ggggctgaag aaaggcgatc
    15721 gcgttgcgtt gatgatgcct aacttattgc aatatccggt ggcgctattt ggcattttgc
    15781 gtgccgggat aatcgtcgta aacgttaacc cgttgtatac cccgcgtgag cttgagcatc
    15841 agcttaacga tagcggcgca tcggcgattg ttatcgtgtc taactttgcc cacgctctgg
    15901 aaaaagtggt tgataaaacc gccgttcagc acgtaattct gacccgtatg ggcgatcagc
    15961 tatctacggc aaaaggcacg gtagtcaatt tcgttgttaa atacatcaag cgcttggtgc
    16021 cgaaatacca tctgccagat gccatttcat ttcgtagcgc actgcataac ggctaccgga
    16081 tgcagtacgt caaacccgaa ctggtgccgg aagatttagc cttcctgcaa tacaccggcg
    16141 gcaccacagg tgtggcgaaa ggcgcgatgc tgactcaccg caatatgctg gcgaacctgg
    16201 gacaggtcaa cgccacctat gggccgttgc tgcatccagg taaagaactg gtagtaacgg
    16261 cactgccgct gtatcacatt tttgccctga ccattaactg cctgctgttt atcgaactgg
    16321 gtgggcagaa cctgcttatc actaacccgc gtgatattcc agggttggta aaagagttag
    16381 cgaaatatcc gtttaccgct atcactggcg ttaacacctt gttcaatgcg ttgctgaaca
    16441 ataaagagtt ccagcagctg gatttctcca gtctgcatct ttccgcaggc ggtgggatgc
    16501 ctgttcagca agtggtggca gaacgctggg tgaaactgac cggacagtat ctgctggaag
    16561 gttatggcct gaccgagtgc gcgccgctgg tgagcgttaa cccgtatgat attgattatc
    16621 atagcggtag catcggtttg ccggtgccat cgacggaagc caaactggtg gatgacgacg
    16681 acaacgaagt accaccgggt caaccgggtg agctttgtgt caaaggaccg caggtgatgc
    16741 tgggttactg gcagcgtccg gatgctacag atgagatcat caaaaatggc tggttacaca
    16801 ccggcgacat cgcggtgatg gatgaagaag gattcctgcg cattgtcgat cgtaaaaaag
    16861 acatgatttt ggtttccggt tttaacgtct atcccaacga gattgaagat gtcgtcatgc
    16921 agcatcctgg cgtacaggaa gtcgcggctg ttggcgtacc ttccggctcc agtggtgaag
    16981 cggtgaaaat cttcgtagtg aaaaaagatc catcgcttac cgaagagtca ctggtgactt
    17041 tttgccgccg tcagctcacg ggatacaaag taccgaagct ggtggagttt cgtgatgagt
    17101 taccgaaatc taacgtcgga aaaattttgc gacgagaatt acgtgacgaa gcgcgcggca
    17161 aagtggacaa taaagcctga gcgttaagtc agtcgtcaga cgccggttaa tccggcgttt
    17221 tttttgacgc ccactaaaga gaaaacaatt tgaattacca aatgattacc acggacgatg
    17281 cgctggcttc tttgtgtgaa gccgtccgtg tctttccggc gatagccctg gatactgaat
    17341 ttgttcgtac gcgcacttat tacccgcagc tggggttgat tcaacttttc gatggcgagc
    17401 atctggcgct aattgatcct ctcgggatca ccgactggtc accgctgaaa gcgatcctgc
    17461 gcgatccgtc catcacaaaa tttctccatg caggcagtga agatctggaa gtgttcctca
    17521 atgtctttgg cgaattacca caacccttga ttgacacgca aatccttgct gccttctgcg
    17581 gacgcccgat gtcatggggt ttcgcttcca tggtggaaga gtattccggc gttacgctgg
    17641 acaagagtga atcgcgcacc gactggctgg ccagaccgct gaccgaacgt cagtgtgaat
    17701 acgcagcggc ggatgtctgg tatctgttac cgatcaccgc caagcttatg gtagaaacgg
    17761 aggcctccgg ctggctacct gcggcgctgg atgaatgccg cctgatgcaa atgcgtcgtc
    17821 aggaagtcgt tgcgccggaa gatgcctggc gtgatatcac caatgcctgg cagttacgca
    17881 cacgccaact ggcctgtctg caactgttag ccgactggcg actgcgcaag gcgcgagagc
    17941 gtgatctggc ggtgaacttt gtcgtgcgtg aagagcattt gtggtcggtc gcgcgttata
    18001 tgccgggaag tttaggcgaa ctggacagcc tgggtttatc cggtagcgaa atccgctttc
    18061 acggtaaaac gctgctggcg ctggtggaaa aagcgcagac attgccggaa gatgccttac
    18121 cgcagccgat gcttaacctg atggacatgc ctggttatcg taaagcgttt aaagcgatta
    18181 agtcgctgat tactgacgtg agcgaaacgc ataagatcag cgccgaattg ctggcatcgc
    18241 gtcggcaaat caaccaactg ctgaactggc actggaaact gaaaccgcag aacaatttgc
    18301 cggagctgat ttccggctgg cgtggtgagc tgatggcgga agcattacac aatttattgc
    18361 aggaatatcc gcagtaaaat cttccgaagc cggactgggc gcgctcagcg ccacatccgg
    18421 ctttggcaaa ctacaaatcc aacaccaggc gtttaccctt cgcacgcgaa caacagatca
    18481 acatactttg ctggctggca cgctcttcat cgctaaaata ctgatcccga tggtcagctt
    18541 caccttccag tattgccgtt tcgcaggttc cgcatacccc ttcacgacat aaacattcca
    18601 ctttcgcggc tttattattt tcaataacct gcaaaatagt catctcttcc ggcaccacaa
    18661 actcttttcc ggaacgggca agcaccaggg taaatgcatc gccggttttg tcttcgatag
    18721 caaattgctc aaagtgcagt gtatcggcgg cgatgtccag acgcgcagct tcacttctta
    18781 ccgcttcaat tagcgcctcg gggccacagg tataaacgtg tgtgccaggt tcgatatccg
    18841 ccaataatcg cgctaattcc agtcgtgttc cggttgatga atgatgcaaa tggactttct
    18901 cagcctgcgg atgctggact agctcagcac gaaatgcgca actttctgga tttcgcgagc
    18961 agtaatgtag ctgccagtcg atgtcgctgt gttgcaactc tgccatgtgc gacaggaaag
    19021 gggggatacc aataccgccc gcgataaaca gatgctttct ggctgaggga attagcgcaa
    19081 acaggttatt aggcgttgag atcgttaacc gatcgcccac ttttacctgc tgatgcaaaa
    19141 agcgggaacc gccgcgcgag ttttcttcca gtcgaacggc aatctgataa ctagaggtgt
    19201 tatgcggtga actcagtagt gaatacgcat tgctgtactg gttatcacca tcgctcatct
    19261 gcactaggat gtgacttcct ccggtgaacg caggcagcgg tttgccatcg gttgccacca
    19321 gcgtgaagcg tttcacctgt tcggtaaggg gttcaacctg gctcacctgt acttcaaaca
    19381 tttgatagtc tgacattgct gtactccatc aaaaatcagc ccagcggcgg aaaataccgc
    19441 cgccgatgtc attagtcctt aaacacctgc gccagcagat tatggaaatg ggcgatacca
    19501 tgttcggaaa tgccactacc gctactgtcg gccatgatgc gcccctgacc acgatagcca
    19561 cgcgatttca gccctttctg tacgctttca accagacgta aatcttccgg atggaacaca
    19621 tcgcgatacc actcaatcag cgatttttgc tcgtcggtta actcttcatt ggtgaagtaa
    19681 atatcgtagt tttgcagggt agtttcagaa tccaccggga attcataaat gaccgtcatc
    19741 atccctttga tcggggtgac gttcagcatc gtgcacggcc acagccagaa accgtggaat
    19801 gccgcatccg taccctcttc aaatttaaac gactgttcgg acggtttggc gaaaccgtat
    19861 tgcagcgtcc agttaccgtg catggtgtgc cagtaacgat caacctgtac ggagtcggag
    19921 aaacctagat gcgccggacc acagtgatag cactcgagat agttatcgac aatgttcttc
    19981 cagttggcag gcgtgcgggt ggtaaagcgg gccgctagtt tcagattgtg gacttccggg
    20041 caggcttcca gcactttcgc gcccagaccg gtgtattgat tcacttgaag tacgaaaaaa
    20101 aaccgggagg acattggatt attcgggttc tgatgggatt agatttggtg gggcttgcaa
    20161 gcctgtagcg caaattttag tcgttaatca atgaaacgcg aaagatagta aaaaattgct
    20221 tttgtttcat tgaaaatacg aaaaacaaaa acactgcaaa tcatttcaat aacagcttca
    20281 aaaaacgttc aaaaccgata acaaccaggc tgtaaccaaa tgactcatat cacaaatcag
    20341 ctcatgccgt ttaggtatgt tacatgtgtg attatgtgag gtgaagtatg ttttagctgg
    20401 ttcatggttg ttatacggct ttttttacat cttgtggttc ttgtgaagga gctacaagac
    20461 cttcctgttc ataaatacca tactttgaca aaatcttttt acgagtatct gttggtaatg
    20521 attcaagcat tttggctgtt tgtaaggttt gctgcgaaat accttgcaaa tcagcctgga
    20581 tggaatcatc ggaagaaaaa atctcaggca taagagcttc aaatcctttc tttctgaaca
    20641 actcaatcgc agaatgcagt tccttgtcct ctaacaattc gacaattgcg atcagtttag
    20701 ttttcaatgt gctgcggtca ggttgagaat ttttattctc ttgattattt ttatgctcaa
    20761 ccattggcat gctctgactt tggataaacc actctattgg acgccctgtt gcgtaggcta
    20821 tttgtgctaa tcgatctaaa gacgggtatg tcttgccaga caggtagttt ctgataaccc
    20881 catcagaaag tccgcactta gctgcaaacg cccggcttga ttctccattc attgcctctt
    20941 caaggcgttc cttgaaacgc attatttccg ggggcataaa aatgcgctct gcatttttgc
    21001 tttctttttc acctgtcatg cccaatctct ctgttatata aagaatttta cagaattctt
    21061 tgatttgcac tggttaaaat aaaaatgagc atagctcttg aattgagcat ttttctgcgg
    21121 tatcgttttt cccgatagcg gtagccggta tcagttaccg aaaacgttaa gagagtaagg
    21181 atcgcagaat gtcagcaaaa aaccaagact ggcacgcaga gcagataaag gctgctgttc
    21241 gtatgaaggg gaagacacta tctcaattat ccagaga
//
LOCUS       DAEEGA010000066        21255 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEEGA010000066 DAEEGA010000000
VERSION     DAEEGA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21255
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.066"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..793
                     /locus_tag="KLH46_002728"
     CDS             <1..793
                     /locus_tag="KLH46_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBE0379354.1"
                     /translation="VAAQPTIVPQLQQQVTGLTSSLNSQEKKELTHKLESIFNNTQVQ
                     IAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQVGYGLEEK
                     VTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSESESTSSTD
                     HYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDKTFSIAVFS
                     FFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGASGRW"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="KLH46_002729"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="KLH46_002729"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBE0379355.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="KLH46_002730"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="KLH46_002730"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBE0379356.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="KLH46_002731"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="KLH46_002731"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBE0379357.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /locus_tag="KLH46_002732"
     CDS             complement(4991..7225)
                     /locus_tag="KLH46_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBE0379358.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="KLH46_002733"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="KLH46_002733"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBE0379359.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="KLH46_002734"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="KLH46_002734"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBE0379360.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="KLH46_002735"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="KLH46_002735"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBE0379361.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="KLH46_002736"
     CDS             complement(12980..14320)
                     /locus_tag="KLH46_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBE0379362.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="KLH46_002737"
     CDS             complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="KLH46_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBE0379363.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16108..16557)
                     /locus_tag="KLH46_002738"
     CDS             complement(16108..16557)
                     /locus_tag="KLH46_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBE0379364.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="KLH46_002739"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="KLH46_002739"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBE0379365.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRYLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="KLH46_002740"
     CDS             complement(17357..17686)
                     /locus_tag="KLH46_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBE0379366.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18308..18853)
                     /gene="syd"
                     /locus_tag="KLH46_002741"
     CDS             complement(18308..18853)
                     /gene="syd"
                     /locus_tag="KLH46_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBE0379367.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18921..19769
                     /gene="queF"
                     /locus_tag="KLH46_002742"
     CDS             18921..19769
                     /gene="queF"
                     /locus_tag="KLH46_002742"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBE0379368.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19881..21245
                     /gene="ppnN"
                     /locus_tag="KLH46_002743"
                     /gene_synonym="ygdH"
     CDS             19881..21245
                     /gene="ppnN"
                     /locus_tag="KLH46_002743"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBE0379369.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5226 a         5670 c         5073 g         5286 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctggt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc atgccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccgaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatat
    16861 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18181 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18241 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18301 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18361 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18421 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18481 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18541 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18601 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18661 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18721 tagttgaaat aatgcacggt gaaggaacac cgtacaattc ctcacttaac ggccagcttt
    18781 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18841 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18901 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18961 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19021 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19081 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19141 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19201 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19261 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19321 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    19381 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagcggtg
    19441 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19501 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19561 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19621 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    19681 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19741 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19801 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19861 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19921 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19981 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20041 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20101 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20161 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccataata
    20221 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20281 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    20341 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc
    20401 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg gaagcgccga
    20461 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    20521 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20581 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20641 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20701 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    20761 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    20821 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20881 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    20941 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21001 agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt gcgttctccg
    21061 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    21121 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21181 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21241 cgtaatctcc cctcc
//
LOCUS       DAEEGA010000067        21250 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEEGA010000067 DAEEGA010000000
VERSION     DAEEGA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21250
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.067"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..394
                     /locus_tag="KLH46_002744"
     CDS             <1..394
                     /locus_tag="KLH46_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBE0379370.1"
                     /translation="IELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQ
                     MIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPT
                     RRGRAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(432..1430)
                     /gene="fbp"
                     /locus_tag="KLH46_002745"
     CDS             complement(432..1430)
                     /gene="fbp"
                     /locus_tag="KLH46_002745"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBE0379371.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1606..2979
                     /gene="mpl"
                     /locus_tag="KLH46_002746"
     CDS             1606..2979
                     /gene="mpl"
                     /locus_tag="KLH46_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBE0379372.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(3062..3613)
                     /locus_tag="KLH46_002747"
     CDS             complement(3062..3613)
                     /locus_tag="KLH46_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBE0379373.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3707..5059
                     /gene="pmbA"
                     /locus_tag="KLH46_002748"
     CDS             3707..5059
                     /gene="pmbA"
                     /locus_tag="KLH46_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBE0379374.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            5242..5628
                     /gene="cybC"
                     /locus_tag="KLH46_002749"
     CDS             5242..5628
                     /gene="cybC"
                     /locus_tag="KLH46_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBE0379375.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5673..6137)
                     /gene="nrdG"
                     /locus_tag="KLH46_002750"
     CDS             complement(5673..6137)
                     /gene="nrdG"
                     /locus_tag="KLH46_002750"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBE0379376.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(6295..8433)
                     /gene="nrdD"
                     /locus_tag="KLH46_002751"
     CDS             complement(6295..8433)
                     /gene="nrdD"
                     /locus_tag="KLH46_002751"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBE0379377.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8827..10482)
                     /gene="treC"
                     /locus_tag="KLH46_002752"
     CDS             complement(8827..10482)
                     /gene="treC"
                     /locus_tag="KLH46_002752"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBE0379378.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10532..11953)
                     /gene="treB"
                     /locus_tag="KLH46_002753"
     CDS             complement(10532..11953)
                     /gene="treB"
                     /locus_tag="KLH46_002753"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBE0379379.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(12072..13019)
                     /gene="treR"
                     /locus_tag="KLH46_002754"
     CDS             complement(12072..13019)
                     /gene="treR"
                     /locus_tag="KLH46_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBE0379380.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            13204..13257
                     /gene="mgtL"
                     /locus_tag="KLH46_002755"
     CDS             13204..13257
                     /gene="mgtL"
                     /locus_tag="KLH46_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBE0379381.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            13398..16094
                     /gene="mgtA"
                     /locus_tag="KLH46_002756"
     CDS             13398..16094
                     /gene="mgtA"
                     /locus_tag="KLH46_002756"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBE0379382.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(16300..16686)
                     /gene="ridA"
                     /locus_tag="KLH46_002757"
     CDS             complement(16300..16686)
                     /gene="ridA"
                     /locus_tag="KLH46_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBE0379383.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16759..17220)
                     /gene="pyrI"
                     /locus_tag="KLH46_002758"
     CDS             complement(16759..17220)
                     /gene="pyrI"
                     /locus_tag="KLH46_002758"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBE0379384.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(17233..18168)
                     /gene="pyrB"
                     /locus_tag="KLH46_002759"
     CDS             complement(17233..18168)
                     /gene="pyrB"
                     /locus_tag="KLH46_002759"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBE0379385.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(18172..18306)
                     /gene="pyrL"
                     /locus_tag="KLH46_002760"
     CDS             complement(18172..18306)
                     /gene="pyrL"
                     /locus_tag="KLH46_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBE0379386.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18587..18982)
                     /locus_tag="KLH46_002761"
     CDS             complement(18587..18982)
                     /locus_tag="KLH46_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBE0379387.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(19114..19827)
                     /gene="bdcA"
                     /locus_tag="KLH46_002762"
     CDS             complement(19114..19827)
                     /gene="bdcA"
                     /locus_tag="KLH46_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE0379388.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19898..20491
                     /locus_tag="KLH46_002763"
     CDS             19898..20491
                     /locus_tag="KLH46_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE0379389.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20636..21088
                     /gene="tabA"
                     /locus_tag="KLH46_002764"
     CDS             20636..21088
                     /gene="tabA"
                     /locus_tag="KLH46_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBE0379390.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         5086 a         5307 c         5644 g         5213 t
ORIGIN      
        1 aatcgaactg ggatacaggg agataaaaca gacgatgcaa ctgagcaggc tgacgctgag
       61 aagtaaaaag ccggagcttg tggagcaaga actgtggggt gtcttactgg cttataatct
      121 ggtgagatat cagatgatta aaatggcgga acatctgaaa ggttactggc cgaatcaact
      181 gagtttctca gaatcatgcg gaatggtgat gagaatgctg atgacattgc agggcgcttc
      241 accgggacgt ataccggagc tgatgcgcga tcttgcaagt atgggacaac ttgtgaaatt
      301 accgacgaga aggggaaggg ccttcccgag agtggtaaag gagaggccct ggaaataccc
      361 cacagccccg aaaaagagcc agtcagttgc ttaactgact ggcattactc cgtgtggaga
      421 ggcgcaggag attacgcgtc cgggaactca cggataaagc gttcgacatc ttcaaccata
      481 tggtcgttgc cgacaaagaa cgaacggcgc tggtgcagag tttccgggat gatatccaga
      541 atacgctctt tgccatcgct cgctttaccg cccgcttgtt ccgccaggaa tgccatcggg
      601 ttgcactcat aaagcaaacg cagtttgccg tccgggtggc tggcggtgct tgggtagaga
      661 taaataccgc ctttcagcag gttacggtgg aaatccgcaa ccagtgaacc gatataacgc
      721 gaggtatacg ggcgattggt ggatttatct tcttcctggc agaatttaat gtacttcttc
      781 accccgttcg gaaacttaat gtagtttcct tcgttgatgg agtaggtttt gcctttctcc
      841 gggaagcgca tccgttcctg gcacaggcag aaaacgccca gcgaaggatc gtaagtaaag
      901 gcgtgaacac cgcatccggt ggtgtaaacc agcatggtag aggagccgta taccacgtaa
      961 cctgccgcaa cctgtttgtt accaggctgg aggaaatctt cttccgttac cggcgtgcca
     1021 acaggcgtaa cgcggcggta gatggagaaa atggtaccga cagagacgtt aacatcgatg
     1081 ttggacgagc catccagggg gtccatcagc accacgtatt ttgcgtgttc acagccttca
     1141 aagacgacaa tctcatcttc ttcttcagag gcaatccccg caacgatatc gcgtgctttc
     1201 agtgcggctt tcagtttttc attagcgaac aagtcgagtt tttgctgaac ctcgccctgc
     1261 acgttctcag caccgctggc acccaggata tcaaccagtc ctgctttgtt gatatcgcga
     1321 tggataatct tggcgcccag ttttattgcc gacagcaaag cagtgagctc accggtagca
     1381 tgagaaaact cgtgctgctt ttcgacaata aattcaccta acgttttcat aaaactttcc
     1441 ctgcaatgtt tatggagtaa agcgaccgca acaatcttaa caaataatct caatgttgcg
     1501 ctcaggtgaa tcgcgccagc aaattgcgga ttatcctgaa atgcgtttct cacttgcccg
     1561 acatatgcgt aaaatgagcg gcagattaaa aaaggatagt gacgtatgcg cattcatatt
     1621 ttaggaattt gtggcacatt tatgggcggt ctggcgatgc tggcgcgcca gttaggccat
     1681 gaagtaacgg gttcggacgc caatgtgtat ccgccgatga gcaccttact tgagaagcaa
     1741 ggcattgagc tgattcaggg ttacgatgcc agccagctcg agccgcagcc ggatctggtg
     1801 attattggca acgccatgac ccgtggaaat ccgtgtgtgg aagcggtact ggaaaaaaac
     1861 atcccttata tgtcaggtcc acagtggctg cacgattttg tgctgcgcga ccgctgggtg
     1921 ctggccgttg ccggtacaca cggcaaaacc accaccgcgg gaatggcgac ctggattctg
     1981 gaacagtgtg gttacaaacc gggatttgtg atcggcggtg tgccggggaa ctttgaggtt
     2041 tcggcgcgtc tgggcgaaag cgacttcttt gttatcgaag cggatgagta cgactgcgcc
     2101 ttcttcgaca aacgctctaa atttgtccat tactgcccgc gtacgctgat cctcaacaac
     2161 cttgagttcg atcacgccga tatctttgac gacctgaaag cgatccagaa acagttccac
     2221 catctggtgc gtatcgttcc ggggcagggc cgtattatct ggccggaaaa tgacatcaac
     2281 ctgaaacaga ccatggcgat gggctgctgg agcgagcagg agctggtggg tgagcagggt
     2341 cactggcagg cgaaaaagct gaccaccgat gcttccgaat gggaagtttt gctggatggc
     2401 gaaaaagtgg gcgaagtgaa atggtcgctg gtaggcgaac ataatatgca caatggcctg
     2461 atggcgattg cagcggctcg ccatgttggt gtagcgccgg cagatgccgc taacgcgctg
     2521 ggttcgttta ttaatgctcg tcgccgtctg gagttgcgtg gtgaagcgaa tggcgtcacg
     2581 gtatatgacg attttgccca tcacccgacg gcgattctgg caacgctggc ggcgctgcgt
     2641 ggcaaagttg gtggtacggc gcgcattatt gctgtgctgg aaccgcgctc gaataccatg
     2701 aaaatgggga tctgcaaaga cgatctggca ccttcattag gtcgtgccga tgaagtcttc
     2761 ctgctgcaac cggcgcatat tccgtggcag gtggcagaag tggcagaagc ctgcgttcag
     2821 cctgcacact ggagtggcga tgtggatacg ctggcagata tggtggtgaa aaccgctcag
     2881 cctggcgacc atattctggt gatgagcaac ggcggttttg gtgggatcca tcagaaactg
     2941 ctggatggtc tggcgaagaa ggcggaagct gcgcagtaat tcggcttcag cctgatatag
     3001 cattgccgga taaggcgttt acgccgcatc cggcaaacca tactcacatc aacaacgaaa
     3061 attacccttc attctccgcc agttcgcgca gatactggaa aatctggcgt gcggatttcg
     3121 gcggcttatt cccttctttc tctttcttcg cgttgcggat cagtgtacgc agttgctgac
     3181 gatcggcatc gggccacaga ttcaatactt cagcgatggc gtcatcacct tgatcgatca
     3241 gacgatcgcg caggttttcc agtttatgga acagcaccac ctgctggttg tgacggtttt
     3301 tcagcttatc cagcgcctga cgaataggct ctacgtcgcg ctggcgcagc atcttaccga
     3361 tgagctgcaa ctggcggcgg cgaccttcca tcttaatacg ctgggcgagc tcaatagccg
     3421 ggcgcagatc cgcatctaac gggatcttat ccagcgcgtt tttccccaga tcaacaattt
     3481 ccgcgccaag gcgttttagc tcctcggcat cacgtttaat ttcactttta ctgacccaga
     3541 taatttcatc gtcttcgtct tcgatgtcat caccgggaac gtcgtcgagc cagtcttcgg
     3601 gctgcttagt catctcaggc tccttaaaaa aagaggctaa tgttaccagt taagatgcgc
     3661 actgaaaaac ggttctctgt tagacttcag agaaactctc tacattatgg cacttgcaat
     3721 gaaagtaatc tctcaagttg aagcgcagcg caagattctg gaagaagcag tttccactgc
     3781 gctggagttg gcctcaggca aatcggacgg tgcggaagtt gccgtcagca agaccaccgg
     3841 cattagcgta agcacgcgtt atggtgaagt ggagaatgtt gaattcaata gtgatggcgc
     3901 gctggggatc actgtttatc accagaaccg taaaggtagc gcatcgtcta ccgatttaag
     3961 cccgcaggcc attgcccgca ccgtacaggc ggcgctggat attgcccgtt atacctcgcc
     4021 agatccctgt gccggcgtgg cagacaaaga gctgctggcc tttgacgcac cgaatctcga
     4081 cttgttccac cctgcggaag tttccccgga tgaagccatt gaactggcgg cccgcgcaga
     4141 acaggcggca ttgcaggcgg acaaacgcat caccaatacc gaaggtggca gctttaacag
     4201 ccactacggt gtcaaagttt ttggcaacag ccacggcatg ttgcagggtt actgctcaac
     4261 gcgtcattcg ctctccagct gtgtaattgc cgaagaaaat ggcgatatgg agcgtgatta
     4321 cgcctacacc attggccgcg cgatgagcga tctgcaaacg ccagagtggg tcggggccga
     4381 ctgtgctcgc cgtactttat cgcgcctgtc accgcgtaaa ctctccacca tgaaagcgcc
     4441 ggtcattttt gccaatgaag tggcaaccgg gctttttggc catctggtgg gggcgatagc
     4501 gggtggctcg gtttaccgta aatctacctt tctgctggat tcgctgggta aacaaattct
     4561 gccggactgg ctgaccattg aagagcatcc gcatctgctg aaagggctgg cgtcgacgcc
     4621 attcgacagc gaaggtgtgc gcaccgagcg tcgcgatatt attaaagatg gcatcctgac
     4681 tcagtggctg ctgaccagct actcggcgcg gaaactgggg ctgaaaagca ccggacatgc
     4741 gggcggtatt cacaactggc ggattgccgg acaagggcta agcttcgaac aaatgctcaa
     4801 agagatgggc accgggctgg tggtgacgga gctgatgggc cagggcgtga gtgccattac
     4861 cggtgattat tcccgtggtg cagcgggctt ctgggtagag aatggcgaaa ttcagtatcc
     4921 cgttagtgaa atcaccatcg caggtaattt aaaagatatg tggcgcaata ttgtcaccgt
     4981 cggtaacgat attgaaacac gcagtaatat acagtgtggt tctgtgctgt tgccggagat
     5041 gaaaatcgcc gggcagtaaa aaactggcgc gacctgccgc gctataaact aaaaaattac
     5101 acaaaatcat tcgcactgca tcgagacgga aagtgaatga gggccgggga gcgtactcgc
     5161 agtacgtgac ccgggcgaat gagcgtagcc gtcgatgagg cagtgtgaag gatgaagtgt
     5221 aaataaaaaa ggaagtgagc aatgcgtaaa agcctgttag ctattcttgc agtctcctcg
     5281 ttggtattca gttctgcgtc gtttgccgct gatcttgaag acaatatgga aaccctcaac
     5341 gacaatttaa aagtgatcga aaaagcggat aacgcggcgc aagtcaaaga cgcgttaacg
     5401 aagatgcgcg ccgcagcgct ggatgcgcaa aaagcaacgc cgccgaagct cgaagataaa
     5461 tcaccggaca gcccggaaat gaaagatttc cgccacggtt tcgacattct ggtcggtcag
     5521 attgacgacg cgctgaagct ggcaaatgaa ggtaaagtaa aagaagcgca ggctgctgca
     5581 gagcaactga aaacgacccg caacgcctat caccagaagt atcgttaatt cctcatttcc
     5641 ctgttgcctg cactcaggta acagggaact tatcatcgca aatgatgcac cacctgattg
     5701 ctgctgccgc gccagatcag ggaagggtct tttaaatcct gcacaaattt gccgtcgacc
     5761 agcacgttaa tcaaatcaac aacctgcatt tgcgcagcgt tgagttcgtc gagtttatag
     5821 cctgtccaca cccagatgtc tttacccgga cactcggcgc ggatgcgttt taccagtttc
     5881 agaatatccg gcacgttttg cggatgcagc ggatcgccgc cggagaggga aatcccctgg
     5941 cgtttgatac gagtgtcatt cagatcttta atgatcttgt cttccattgc tttggtaaat
     6001 ggctgaccag aatttacccg ccatgtgctt ttgttatagc aaccggggca ttcatgaaca
     6061 cacccggaga caaacagggt gcagcgagtg ccggggccgt tgacgatgtc gacaggatag
     6121 tactgatgat aattcattat ttcgcctggc ttatcagtcc gcgtgtaggc tgcatccggt
     6181 aatcaatgcc tgatgcgacg ctgtcgcgtc ttatcaggcc tacagccgtt gccaaatgta
     6241 ggccggataa ggcgtttacg ccgcatccgg catttgctta gcgcagagtg aagattaacc
     6301 tatctgccca ttccccaaat gtttaacgcg gcgcttaact tcttcctgct taccagcgtt
     6361 aaacggacgt gcatccgggc tacctaaata tccgcacacg cggcgagtta ccgacacacg
     6421 ggaggcgtca tggttaccac atttcgggca agtgaagcct ttgctggtgc actcgaactc
     6481 accggtaaag ccacactcgt agcactcatc aatcggtgta ttggtgccgt aatacggtac
     6541 atgctgatag ctgtaatccc agacatcttc cagcgctttc aggttgtgct gaatgtttgg
     6601 atactcgccg tagcaaatga aaccaccgtt cgccagcggc gggtaaggcg cttcaaagtc
     6661 gatcttgtcg tacgggttca ccttcttctc cacatcaagg tggaaactgt tggtgtagta
     6721 acctttatcg gtcacgcccg gcaccacgcc aaactcagca gtatcgagac ggcagaagcg
     6781 gtcgcacagg ttttcactcg gcgtgctgta aagactgaaa ccataacccg tttcttcttt
     6841 ccactgatcc actgcctgac gcagacgttc aacaatggcg atacctttcg cgcgaagctt
     6901 ctcgttgtcg taaacatgct cgccgctgaa cagcgcgtta atggtttcgt ggatgccgat
     6961 gtaacccagc gaaatagacg cacgaccgtt tttgaagatt tcagaaacat catcgtcagc
     7021 attcagacgt acgccacaag caccttccat atagaggatc ggggcgacgc gcgctttcac
     7081 gccttcgaga cgagcgatac gggtcatcag tgccttacgt gccagcacca gacgttcatc
     7141 cagcagcttc cagaaggtgg cttcatcgcc ttttgcttcc agagcaatac gcggcaggtt
     7201 caggctgatc acgccgaggt tgttacgacc atcgtggatc tgctcgccgt tttcattttc
     7261 ccacacgccg aggaagctgc ggcagcccat cggggtttta aacgaaccgg tgacgttcac
     7321 tacctgatcg tagttcagga tatccggata catgcgcttg cttgcgcact ccagcgccag
     7381 ctgtttgatg tcgtagttcg gatcgccttt tttatggttc aggccatcgc gaatcgcaaa
     7441 caccagtttc gggaacaccg cagttttacg gtttttaccg aggcctgcga tacggttacg
     7501 caggatcgat tcctgaatca ggcgcgattc ccagctggtg cccagaccaa aaccaaaggt
     7561 tacaaacggc gtctgaccgt tggcggtgtg cagggtgttt acttcgtact ccagcgactg
     7621 gaaggcatcg tagcactctt tgatggtacg agagttagcg tagccttcgg cgtccgggat
     7681 gttccactct tctgcggtct tgcgatgttt gttgtagctg gcggtaacaa acggcgccag
     7741 cacttcatcg atacggttaa tggtggtgcc gccataaata tggctggcga cctgagcaat
     7801 aatctgcgca gttaccgcgg ttgccgtaga gatcgacttc ggcggttcaa tctcggcgtt
     7861 ccccatttta aaaccctggg tcagcatgcc tttcaggtcg atcaacatgc agttgaacat
     7921 cgggaagaac ggcgagtaat cgagatcgtg atagtgaata tcgccacgct catgtgcctg
     7981 caccacgtca cgcggcagca ggtgctggcg tgcatagtgt ttagccacga tcccggccag
     8041 caggtcgcgc tgggttggaa tcaccttgct gtctttgttg gcgttttcgt tgagtaacga
     8101 ggcgttggtc tgctcgacca gaccacggat ctcctggttc aggcgaccgc gtttttcacg
     8161 ttcaatgtcg cgatcgtgac ggtactcgat gtaagcacga gccagttgtt tgtatggacc
     8221 cgacatcagc tggttttcaa ctgcggtctg gatctcgttg atatccacct ggttgcgccc
     8281 ctgcatctgc tcgttgacaa ccgcggcaac agtggcgcaa taatcggcat catcgacttc
     8341 cgctgcttta gctgcacgca gaatcgcttc tttgatgcgc tctgatttaa acggcacttt
     8401 gcagccgtct cgtttcatca catgcggtgt catgatcgct ccatattttt aagaacaggt
     8461 tatccacaga aattgggaaa ggcgttttcc ggttttttcg tttcgttttc cggtgctttc
     8521 cgcaatcgcc atccgcttat ccacaacgcc gcaccttttt cgtggggagc tgtagtagca
     8581 attatagtcg attaatacaa catattgggt tgggatgcat tttaaagtct atatatagtg
     8641 ctttgcatca aggatgtttt aacttttttt gatgtagctc aaagtaaaaa gcagagcgta
     8701 cggatgacgg gcgctacagc gttatgtaaa ttttttaatg aatttgctgg ttgaaaaatc
     8761 aacaaaaaca acatgctgac agacaaaacc ccggaatgac cggcattatc ggggcttagg
     8821 gaagatttac ttctgtaacc accagacagc ctcaaaaggc cgtaaattca tggcacaggg
     8881 ttgtggtgag gcttcttcgt agttatgcat cacaagctgc cagttgccgc gcatttgccc
     8941 tggctgccag ggttggatct cacggctaag gttggcaatg accagcaagg tttgcccctt
     9001 ccattcacgg cgatagcacc acaatacagg gctgtttggc agcagatcct ggtaattgcc
     9061 ccatgtcagg atggcttctt gcttacgcag tgcgattaac ttttggtagg tgtaaaacac
     9121 agaggagtca tcggccagcg cggcttctac gttgatttgt tgatagttat cgcctaggcc
     9181 aatccacggt tcgccagccg taaacccggc attatcgccg ttgctccatt gcatgggcgt
     9241 gcggctgttg tcacgggatt tactggcgag gattgccaat aactcgtcgg catcacgacc
     9301 atcgttgcgc agctcggcaa acatattgag gctctccacg tcgcgatagt cagtaatgcg
     9361 cgtgaaatgc gggttggtca tgccaatctc ttcgccctgg tagatatacg gcgttccctg
     9421 catgccatgc agcaccatcg ccagcatttt tgccgcaggc acgcggtatt caccttcatc
     9481 accaaagcga gaaacaatgc gcggctgatc gtggttacac cagaacaagg cattccatgc
     9541 cacgttgtgc attccttgtt gccagtggcg gaacaatgtt ttcaacgcca caaagtcagg
     9601 tttagccagc gtccattttt caccaccggg ataatcgacc ttcaggtgat gaaaattaaa
     9661 ggtcatcgac aattcactgc ctgtcagtgc cgcgtatcgc tggcaatgct caaggctggt
     9721 ggaggacatt tcacctacgg tcattaaccc gcgtggcgta aacacatcgc ggttcatctc
     9781 gtgcaaaaac tcgtgtgctc gtggcccgtc ggtgtagaag cgacgcccgt cgccatccag
     9841 gtcttcaggg aaacgcgggt ctttggagat cagattcacc acatccaggc gcaacccgtc
     9901 gaccccacga tcggcccaga attcacagac ttttttcagc tctgcgcgta ccgctggatt
     9961 ctcccagttg agatccgcct gttctggtgc aaagagatgc aaatagtact gttcgctttc
    10021 cgcatgccag cgccacgcac taccgccaaa ttttgaacgc cagttgttcg gtggcgtttc
    10081 tggttcgcca tcgcgccaga tataaaactg gcggtaaggg ctttctttgt tcagcgcctc
    10141 gcgaaaccag gcatgttggg tagaggtatg gttaaacacc atatcgagaa tgatacgaat
    10201 cccgcgcgat tttgcctgcg tcaccagttc gtcaaaatcg tccagcgtgc cgtaggtggg
    10261 atcaatcgcc gtatagttcg ctacatcgta accgttatcg acctggggag agacataaaa
    10321 gggggttagc cagatggcat caacgcccag tttatgcaga tagtccaggc gttggataac
    10381 gccacgtaaa tcgccggtgc cgctacccgt ggtgtcctga aaactctttg gataaatctg
    10441 gtagataacg ccgttttgcc accagtgggg aagattagtc attacgttat tcctgcaaat
    10501 gcgagggggc gcaattgcgc cccgaagaaa attaaacaat gtccagcgtg cccaggcggt
    10561 atttccgctg atagataaac gaggtgagta caatcgggat gatgatggcg atagccattg
    10621 ccagcgcaaa tacctgccag tagctcggtt gaatagagag aattcccggc aggcccccta
    10681 cgccgatacc attcgccata acgccgttca ggccgcatag caatcctgcc agaccagaac
    10741 caatcatcgc gcacagcatc gggaagcgat atttcaggtt gatgccgtac attgcaggct
    10801 cagtgacccc aagccaggcg gagatagcgg caggcacgga gatctcgcgt tcattgtgct
    10861 tgcggctgga aatgataatg cctatcacgg cggagccctg agcgatattc gacagcgcaa
    10921 tcagcggcca cactggcgtg ccacccatgc tttgaatcat ctgcaaatca atagcaaggg
    10981 tggtctggtg tacgccggtg atcaccagcg gcgcgtacag gaagccaaac aatgccgcac
    11041 caatcggagc aaagctgccg gtcatcaggt gacgtaccgc aaaggcaacg ccatcgccaa
    11101 tcatgcgacc aaacggacca atcagcgcat gggcgaggaa caccgcgagg atcagcgaac
    11161 agacgggcac caccaccaga tagaggtaat ccggtacgat gcgtttaagg cgagtttcaa
    11221 taacgcccag cgccagcccg gctaacagtg ccgggatcac ctgcgcctga tagcccactt
    11281 tggcgatgct gaacatgcca aagtcccaca cttccggcag ctgctgcccg agcagataag
    11341 cgttcatcag ctggggggaa accagtgtca cgccaagcac gataccaagg atcggcgtgc
    11401 cgcccatttt tttcaccgct gaccagcaaa tcccgaccgg caggtagaag aaaatcgctt
    11461 caccgatcaa ccacagaaaa tcgtagatcg tttgcaggga agggtacatt tgcgccagcg
    11521 tctgaccgtt gctcatgggc aaatcgccga tcacattgcg aaaaccgagg atcaaaccgc
    11581 cgctaatcaa cgcgggcagc aacgggaaga agatctccgc gaaatgagag atcaactgct
    11641 catgccattt catattttgc cgggcggctt tttttacctg ctctttatca acctgcgcct
    11701 gtccggttga cgctattagt gcttgatagt aatcacccac gttggtgcca atcaccacct
    11761 gaaattgccc ggcattggtg aaacagcctt tcaccatggg gagttgctca atttctttcg
    11821 gtctggcatt ggccggttgg ttgaggacaa agcgcaggcg agtaatacag tggctcaccg
    11881 tcgcaatatt gccgcgcccg ccgaccagtt caatcaaccg atcgatatcc gtttggttta
    11941 ttttgctcat cataaagccc catggcagat gacatttttg gttggctgca gaatatattg
    12001 cgcggaaaaa tttaaaaacg ggaacgttcc cgaaacgcag cgaagatcac aatttatcgt
    12061 tcaggaaacg atcaggacag ggtggcgggg atgatgattt gttgcggttc gctgcgcccg
    12121 gttacctgtg cgatcaattg gcaggccgcc tggcgtccag cttcggcgta gccaggatct
    12181 acggtgacga tctccggatg aaggaatttc attaacggcg tattacctac gctcgccagt
    12241 tgcaaggtgt cgatgcgttg ctcttgcagg tatttacttg cgccaagagc cagcgtgtcg
    12301 gttgcgcaca gtaacgcggt agtttcaggc gtaatcactt ttgctacgtt ttcatagcct
    12361 tgcttcatag caagccctgg cagagcggca acgggatgca gtttatgcgc tttgcagaac
    12421 gccaggtagg cttcgtgacg tcgcttcccg gtcgtcacgt cactatgcgg cacgccgaga
    12481 taactgatat tgcgatgccc ctggtcatag agccgttgca tcagaatctt gattgcccct
    12541 tcgtcgtcat aacagaccga agcaaagcct tttgcgtcac gcgccagcag aaccagcgat
    12601 gactgccagt gggctaacat ttcttcggtt atgccggtaa aaccgaacag cactacgccg
    12661 tcgatattac gccgtttcag cacccccaaa tgttcggcaa ctaattgtgg ggaaaactga
    12721 ttttccatca tgattggatc gtaaccttgt tcatagaacg ctggcagcat ggtttgaacg
    12781 gcgagatttt ctgacaacga atccagacgg gtaacaatga tggcgaccac tttatcgctt
    12841 tgcccacgca tagcgcgcgc agagcgggaa ggggaaaatc catgctgatt catcactgct
    12901 tcaacacgct cgcgcgtgcg ctgactcacg ccgctttcgt tattcagcac ccgggaaacc
    12961 gtagatttcc ccacgccgct taagcgcgcg atgtctttga tggtcagccg attttgcatc
    13021 ctgttgtccc ataacgtgtt gtttaattat ttgagcctaa ctttacccgt gcattcagca
    13081 atgggtaaag tctggtttat cgttggttta gttgccagca ggtattatat cgccataaat
    13141 gctacgaata ttattggatt ctccttatta tttgcggcgc ttttttcact taccggaggt
    13201 tatatggaac ctgatcccac gcctctccct cgacggagat taaaactttt ccggtaagcc
    13261 cgtcttttca cggcgttacc ggatgcgtaa ggccgtgacg ttttaatgtc cctgctcagc
    13321 tttattacct tcaggtaagg cttcgccacg cctgaagaca tttctgtact gtttcagaca
    13381 gtgcggaggg actccttatg tttaaagaaa tttttacccg gttcattcgc catttacctt
    13441 cccgtctggt tcatcgcgat ccattgcctg gcgcgcagca gacagtgaat acggcggtcc
    13501 cgccgtcctt aagtgcgcat tgcctgaaaa tggcggtgat gcccgaagaa gaattgtgga
    13561 aaacgttcga cacccatccg gaaggattaa atcaggcgga agtggaatct gcccgcgaac
    13621 aacatggtga taataaatta cccgcccaac aaccgtcgcc gtggtgggta catttatggg
    13681 tctgctatcg caaccccttt aatattttgc tcaccattct tggtgctatt tcttacgcca
    13741 cggaagattt atttgccgca ggtgttatcg cgctaatggt cgctatttct acgttgctga
    13801 actttattca ggaagctcgt tccaccaaag cggcagatgc cctgaaagcg atggtcagta
    13861 ataccgcgac ggtactgcga gtaattaacg acaaaggcga aaatggctgg ctggagatcc
    13921 cgatagacca gctggtgccc ggcgatatta taaaactggc ggcgggggat atgatcccgg
    13981 cagatttacg tatcttgcag gcgcgggatc tgttcgtcgc tcaggcgtcg ttaaccggtg
    14041 agtctctgcc cgtggaaaaa gccgctacta ctcgccagcc ggagcacagc aatccgctgg
    14101 agtgcgacac cctgtgtttt atgggcacca ccgtggtgag cggcacggca caagcaatgg
    14161 tgattgctac aggagccaac acctggtttg gtcaactggc ggggcgtgtt agtgagcagg
    14221 aaagcgagcc gaatgccttt cagcaaggga tcagccgcgt tagtatgctg ctgattcgct
    14281 ttatgctggt gatggctccg gtggtgttgc ttatcaacgg ttacaccaaa ggcgactggt
    14341 gggaagcggc actgtttgcg ctttcggtcg cggtaggctt aacgccggaa atgttgccga
    14401 tgattgttac ctcgacgctg gcgcgcggcg cagtaaagct gtcgaaacag aaagtgatcg
    14461 tcaaacatct ggatgccatt cagaactttg gcgcaatgga tattctgtgc actgataaaa
    14521 ccggcaccct gacgcaggac aaaattgtgc tggagaatca taccgatatc tccggtaaaa
    14581 ccagcgaacg cgtgctgcat agcgcgtggt tgaacagtca ttaccagacc ggacttaaaa
    14641 atctgcttga taccgcggtg ctcgaaggta cggatgaaga gtcagcgcgt tcgctggcca
    14701 gccgttggca aaaaattgat gagattccgt ttgatttcga gcgtcgtcgg atgtcggtgg
    14761 tagtggcaga aaataccgaa caccatcagc tggtttgcaa aggtgcattg caggaaatcc
    14821 tcaatgtgtg ttcgcaggtg cgtcacaatg gcgagattgt accgctcgat gacaccatgc
    14881 tgcgtaagat taagcgggtt actgatacgc tgaatcgtca ggggctgcgc gtggttgcgg
    14941 tggcgacgaa atacctgcca gcgcgtgaag gagattacca gcgggcggat gaatccgacc
    15001 tgatcctcga aggatatatt gctttccttg atccgccaaa agagacaacc gctccggcac
    15061 tgaaggcatt aaaagcgagt ggaattaccg taaaaatcct tactggcgac agtgagttag
    15121 ttgcagcgaa agtgtgccat gaagtggggc tggatgcggg agaggtggtc attggtagtg
    15181 atattgaaac gctatctgac gatgaactgg caaatctcgc acagcgcacc acgctgtttg
    15241 cccgcctgac gccgatgcat aaagaacgca ttgtgacttt actgaagcgc gaagggcatg
    15301 tggttggctt tatgggcgat ggtattaatg atgcgcccgc cttacgcgct gcggatatcg
    15361 gcatttctgt ggacggcgcg gtagatattg cccgtgaagc ggctgatatc atcctgctgg
    15421 aaaaaagcct gatggtgctg gaagaggggg ttattgaggg acgtcgcact ttcgccaaca
    15481 tgctgaaata catcaaattg acggcgagtt ctaacttcgg taatgtgttc agcgtgctgg
    15541 tggcgagtgc tttcttgccc ttcctgccga tgttgccgtt acacttgctt attcagaacc
    15601 tgctgtacga tgtgtcacag gtggcgatcc cgtttgataa cgtcgacgac gagcaaattc
    15661 aaaagccgca gcgttggaat ccggcggatc tggggcgctt tatggtcttc ttcggaccga
    15721 tcagctcgat tttcgatatt ctgacgtttt gcttgatgtg gtgggtattc catgccaaca
    15781 cgccggaaac gcaaacgctg ttccagtcgg gctggtttgt ggtgggatta ctgtcgcaaa
    15841 cgctgattgt gcatatgatc cgtacccgcc gtgtgccgtt tattcagagc tgtgcatcgt
    15901 ggccgttaat gatcatgacc gtgatcgtga tgattgtcgg gatcgcattg ccgttttcac
    15961 cgctggccag ttatctgcag ctgcaggcgc tgccgttaag ctatttcccg tggctggttg
    16021 cgattctggc agggtatatg acgttaaccc agttggtgaa agggttctat agccgtcgtt
    16081 acggctggca gtaaagaata aactgggcat ggtggcccgg attcagattg cagacaaagt
    16141 gagctttgtt catgccggat gcggcgtaaa cgcccaatcc ggcctacaaa ttcaataaat
    16201 tgcaggaatc acgtaggcct gataagcgta gcgcatcggg cgattttgct tttgtcatca
    16261 gacttaatcc gggcatgata gcccggattt ccatcaagat tagcgacgaa cagcgattgc
    16321 ttcgatctca atcttcacgt ctttcggcag acgggcaact tcaacgcaag aacgtgccgg
    16381 gaaagtggcg ttgtgttcgg tgaagaaggc ttcgtaagtg gcgtttacgg ttgcgaagtc
    16441 gttcagatct ttcacgaaca cagtggtttt aacgatgtcg cccactttca ggccagcggc
    16501 ttcgacgatt gcttttacgt tatccagcga ctgacgtgcc tgtgcagcga cgtctgccgg
    16561 tacttcacca gttttcggat ttaccgggat ctgaccggag gtgatgatca tattgcccag
    16621 atcaacgccc tgtacgtaag gaccgatagc tgccggtgca ttttccgtcg cgatagtttt
    16681 gctcatgatt tctcctttat tacagcggta aaaagtctgg ctcattatag ggagccagac
    16741 ttttattacc aaccgcaatt aattggccag caccacatta tgggaaaact ctttttcaca
    16801 gtatttgcat ttaagcgcga tgtcattggc gcgttttcgc acggcaaagc tggatgaaac
    16861 cggttcggca tggctgatac agttgctgtt cgggcagacc agcacattgt cgatgcgctc
    16921 tggcagactt gggcgcgatt tacccaccac ttcatagttg tcgatacggt taaccgtggc
    16981 ttgcggtgca tacaatgcca gttggtctac ttgctcttca ctcaaaaagg tattttcgat
    17041 tttgatcaga tctttgcggc ccatctcgcc agaaggcaaa ttcagaccaa tggtgatgcg
    17101 ctgatctgtt tcggtcaact tgaacagact caacagctta aaaccgatct gggcggggat
    17161 atggtcaatt accgtgccgc gtttaatagc ttcaacctgc aatttattat cgtgtgtcat
    17221 ctctatttcc ccttacagta ccagatcgcg attcagaacc agtgccagta acgcctggcg
    17281 agcgaaaatc ccgttgcctg cttgctggaa gtaccaggcg tgaggcgttt tatccacatc
    17341 cgtcgcaatc tcatcaacac gcggcagcgg atgcagcact ttcatattgg ctttggcgtt
    17401 gtggagatcg ctggcgcgaa gaacaaactg cgctttcacg ttggcgtact cggacgggtc
    17461 cagacgctct ttttgcacgc gggtcatgta caggatgtct acttccgcca tcacttcttc
    17521 aatagagctg tgcagactcc aggcgatccc tttttcatcg agcatatcca gaatgtattg
    17581 cggcattgcc agcgcatccg gcgcgataaa gtaaaaacgg ttgccgtcga acttcgctag
    17641 cgcctgggtc agggagtgaa cggtgcggcc atatttcagg tcaccaacca ttgcgacgtg
    17701 gagattgtcc agacgcccct gggtttcctg aatggtgaat aagtccagca aggtttgcgt
    17761 cggatgttgg ttggagccat caccggcatt cagtaccggt acattgccag aaaattcggt
    17821 ggctaggcgc gccgcacctt cctgcggatg acgcatcact atcgcatcga cgtaagtgct
    17881 gataaccgaa atggtatcgg ccagcgtttc gcctttttta cccagtgatg tattggcgct
    17941 gtcggagaag cccaccacgc tggcacccag gcggtgcatg gaagtttcga aagagaggcg
    18001 ggtacgggta gaggcttcga agaaacagct ggcaatgact ttgtgcttca acagctctgg
    18061 ttgcgggttt gctttcagtt tcgccgctgt cgccagcacc agattaaggt catcgcgact
    18121 aaggtcgttt atggaaatga tatgtttctg atatagcgga ttagccatct tttatctcct
    18181 gacacctagg caaaaaaaag cccctcgatt gaggggctgg gaatgggtga tcaacgggaa
    18241 gaaaaacggc aggccagcgt cttttttcag acgcggtaag acaaaatgtc gaacacactg
    18301 aaccatacat cctcccggca aattgtccgg cattatactc atagccagaa gcggtgcaag
    18361 catttgatgc aatattttgt cagcgcaaac ggtttatttg aattaaaagt caaaatatat
    18421 gcatttttat tttctatgat tctggagggg aagtgtcgtt cggtatcaat aattgcagac
    18481 atttcctgct tttttctttt tcattttcac gcaatcagaa atgcatttta ttccattcat
    18541 ccgacttata gggcggaggg tgaaaacgaa cggctaacac tattgcttat agctcaggga
    18601 tacgcgctat cactttaatt tcaaaatcaa agcctgccag ccaggtaaca cccaccgccg
    18661 tccagtttgg ataaggtggg gcgctaaata tttcattttt caccgtcatg atgtcttcaa
    18721 attgtttttc cggatcggta tgaaagctcg taacatcaat gatatcgtca aaagtgcatc
    18781 ccgcagctgc cagggtcgca tgcaaattat caaaggccag tctgacttgt tgctgaaaat
    18841 cgggttctgg tgttccgtcc tctcgacttc ctacttgccc ggaaacaaac agcaaatcgc
    18901 cggaacgaat agccgcagaa taacgatgct cagcatacag tgaatgtcgg ccagcaggga
    18961 aaacagcggt tctttctacc atttggttat cctcaagatt tacgacatga acagaagatt
    19021 tatatttatc gggagccgct tttaggggga cgacgttagt aaacaaaacc cagacatcat
    19081 ggataatggc tgggcttaat tgagcgtagt cggttatgcg ccaaacgcgc catcaatggt
    19141 atgcatcgcg ccggtaacaa aactggcttc tggccctgct aaccatgcga ccataccagc
    19201 gacctcttcc ggttgcccat gtcttttgat ggccataaaa ctatgcaaca tatcgcgcat
    19261 tggcccgttg gcgggattaa cgtcggtatc aattggtcct ggctggacga cgttgatggt
    19321 gatccctcgc ggtccaaaat cacgggccag cccgcgcgcc atgccttgca gggcagattt
    19381 actggcggca taagcagcca tgcctgcaac aggcatacga tcgccattca cggagccgat
    19441 gattaagatg cgcccgcctt cgggcatctg ccgggcggct tcaacagagg catgataagg
    19501 agcatgaata ttgattttga aaaggcgatc aatatcgtcg gcatttaatt ccagggcatc
    19561 gccaaagacg ccaatacctg catttaccac caggatatcc aatgcgccgc tcttacgaac
    19621 gacatcaata acagcgtctc tgtcagcact atctgtgaat actgctgtcg ctccagtctc
    19681 ttgtgccaga cgttcagcgg catctttcga ccccgcatag gtgaatcgta cattggcccc
    19741 atcggtgacg aaacgacgta cgatagcggc accgattcca cgactgccac cgaggatgag
    19801 aactgtctta ccggtaaaag cgcccataag gactccttga tttattatgt aacatgcatt
    19861 acaaaactgt tttaactttc tgtcaacagg ttttgtaatg gtcactaaaa aacaatctcg
    19921 cgttccaggt cgtcccagac gtttcgctcc tgagcaggca gtctctgcgg caaaagtgct
    19981 ttttcaccaa aaaggtttcg atgcagtaag tgtctctgaa gttactgatt atcttggtat
    20041 taaccccccg agtctctacg cggcttttgg cagtaaggct gggttattta gccgtgtact
    20101 caatgaatat gtcggtacgg aagctattcc gcttgccgat attcttcgtg atgatcgtcc
    20161 ggtaggcgag tgcctggttg aggtattaaa agaagcggcg cgcagatata gccaaaacgg
    20221 cggctgcgct ggctgtatgg ttcttgaagg tattcatagt catgatccac aagcgcgtga
    20281 tattgccatt caatattatc acgccgcaga aacgaccatt tatgactata tcgccaggcg
    20341 gcatccacaa agcgcacaat gtgtgactga ttttatgagt accgtgatgt cagggctttc
    20401 tgcgaaggca cgggaggggc actccatcga acaactctgt gcaacagctg ccctggcagg
    20461 ggaagcgata aaaactcttc tcaaggagtg atgctggcct tgatccgaaa ggcgggaacg
    20521 cgcctgccga taagttgtga taagacaata attcacgcgt taaggctggc ggaattgatc
    20581 atcttttcgt ataacgatag aaatgaaaca ttattttaat taaggagtgg aaaagatgat
    20641 catcggaaat attcataatc ttcagccgtg gctaccccag gagttacgcc aggcgattga
    20701 gcatatcaaa gcacacgtta cggcagaaac gccaaagggc aagcatgata tcgaaggcaa
    20761 tcgtctgttt tatcttatct cggaagatat gaccgagccg tacgaagctc gccgtgcgga
    20821 gtaccatgcc cgctatcttg acattcagat tgtgttaaaa ggtcaggaag gcatgacctt
    20881 cagcacgcaa cctgcaggcg cgccggatac cgactggtta gctgataaag acatcgcatt
    20941 tttgccggaa ggcgttgatg agaaaacagt tatcctaaat gaaggtgatt ttgttgtgtt
    21001 ttatccgggg gaagtgcata aaccgctgtg cgcagtgggc gcgccagccc aggttcgcaa
    21061 agcagtagtg aagatgctga tggcgtgatg acttttcgcc gtaaataact ccaggtttac
    21121 ggcgagtttg tgaaaagagc gttttttgat atttttttgt gagtaaaatt tgtaatgctt
    21181 agacgttctt attaactcaa ggagttcgtc atgggcaaaa tatcagattt gaattattct
    21241 caacacatta
//
LOCUS       DAEEGA010000068        21094 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEEGA010000068 DAEEGA010000000
VERSION     DAEEGA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21094
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.068"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..60)
                     /locus_tag="KLH46_002765"
     CDS             complement(<1..60)
                     /locus_tag="KLH46_002765"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379391.1"
                     /translation="MASISIRCPSCSATEGVVRN"
     gene            171..1661
                     /locus_tag="KLH46_002766"
     CDS             171..1661
                     /locus_tag="KLH46_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBE0379392.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1713..2870
                     /locus_tag="KLH46_002767"
     CDS             1713..2870
                     /locus_tag="KLH46_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBE0379393.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2836..2958)
                     /locus_tag="KLH46_002768"
     CDS             complement(2836..2958)
                     /locus_tag="KLH46_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379394.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3161..4846
                     /locus_tag="KLH46_002769"
                     /pseudo
     CDS             3161..4846
                     /locus_tag="KLH46_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4884..7244
                     /locus_tag="KLH46_002770"
     CDS             4884..7244
                     /locus_tag="KLH46_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBE0379395.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7301..10084
                     /locus_tag="KLH46_002771"
     CDS             7301..10084
                     /locus_tag="KLH46_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBE0379396.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNELGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYISYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10445..11845
                     /locus_tag="KLH46_002772"
     CDS             10445..11845
                     /locus_tag="KLH46_002772"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBE0379397.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            12001..13536
                     /gene="gadC"
                     /locus_tag="KLH46_002773"
     CDS             12001..13536
                     /gene="gadC"
                     /locus_tag="KLH46_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBE0379398.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            13667..14986
                     /locus_tag="KLH46_002774"
     CDS             13667..14986
                     /locus_tag="KLH46_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBE0379399.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            15284..15325
                     /locus_tag="KLH46_002775"
     CDS             15284..15325
                     /locus_tag="KLH46_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379400.1"
                     /translation="MVQLVDLARCVSF"
     gene            15363..16508
                     /gene="dosC"
                     /locus_tag="KLH46_002776"
     CDS             15363..16508
                     /gene="dosC"
                     /locus_tag="KLH46_002776"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBE0379401.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            16433..17050
                     /locus_tag="KLH46_002777"
                     /pseudo
     CDS             16433..17050
                     /locus_tag="KLH46_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            17308..17640
                     /gene="ddpX"
                     /locus_tag="KLH46_002778"
                     /pseudo
     CDS             17308..17640
                     /gene="ddpX"
                     /locus_tag="KLH46_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            17654..18550
                     /locus_tag="KLH46_002779"
     CDS             17654..18550
                     /locus_tag="KLH46_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HBE0379402.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            18547..19533
                     /locus_tag="KLH46_002780"
     CDS             18547..19533
                     /locus_tag="KLH46_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0379403.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            19526..20452
                     /locus_tag="KLH46_002781"
                     /pseudo
     CDS             19526..20452
                     /locus_tag="KLH46_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(20508..20939)
                     /gene="osmC"
                     /locus_tag="KLH46_002782"
     CDS             complement(20508..20939)
                     /gene="osmC"
                     /locus_tag="KLH46_002782"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBE0379404.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         5682 a         4937 c         4954 g         5521 t
ORIGIN      
        1 gttacgcacc acgccttcag tagcggagca ggaaggacat ctgatggaaa tggaagccac
       61 gcaagcacct taaaatcacc atcatacact aaatcagtaa gttggcagca ttacccttta
      121 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
      181 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      241 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      301 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      361 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      421 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      481 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      541 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      601 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      661 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      721 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      781 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      841 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      901 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      961 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
     1021 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
     1081 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1141 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1201 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1261 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1321 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1381 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1441 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1501 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1561 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1621 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1681 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1741 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1801 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1861 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1921 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaatg
     1981 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     2041 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     2101 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2161 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2221 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2281 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2341 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2401 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2461 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2521 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2581 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2641 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2701 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2761 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2821 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2881 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2941 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     3001 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     3061 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3121 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3181 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3241 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3301 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3361 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3421 aatatccgta aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3481 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3541 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3601 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3661 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3721 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3781 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3841 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3901 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3961 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     4021 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     4081 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4141 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4201 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4261 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4321 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4381 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4441 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4501 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4561 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4621 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4681 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4741 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4801 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4861 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4921 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4981 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     5041 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     5101 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5161 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5221 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5281 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5341 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5401 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5461 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5521 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5581 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5641 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5701 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5761 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5821 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5881 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5941 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     6001 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     6061 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6121 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6181 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6241 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6301 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6361 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6421 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6481 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6541 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6601 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6661 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6721 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6781 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6841 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6901 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6961 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     7021 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     7081 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7141 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7201 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7261 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7321 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7381 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7441 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattgcagga
     7501 agaagacaat gagctcggcg tggctcattt tgtagaacat atgatgttta acggcacaaa
     7561 aacatggccg ggtaataaag tcatcgaaac atttgagtca atgggcctgc gttttggtcg
     7621 cgatgttaat gcctatatca gctatgacga aacggtgtat caggtgagtt tgccgactac
     7681 gcagaaacaa aatctgcaac aagtgatggc aatcttcagt gaatggagta atgccgcaac
     7741 ctttgaaaaa ctcgaagtag acgctgaacg tggcgtaatt actgaggaat ggcgtgccca
     7801 tcaggatgcg aaatggcgca cctctcaggc gcgccgccct ttcctgctgg ctaatacccg
     7861 taatttagac cgtgaaccta tcggcctgat ggatactgtc gccacggtca cgccggcaca
     7921 attgcgccaa ttttatcaac gctggtatca accaaataat atgaccttta tcgtggtcgg
     7981 cgatatcgac agtaaagaag cgctggcgct gataaaggat aatttaagta agcttccggc
     8041 taacaaagca gctgaaaatc gcgtctggcc gacaaaagcc gaaaaccacc tgcgctttaa
     8101 tatcattaat gataaagaaa accgggtgaa cggcatcgca ctctattatc gcctgccaat
     8161 ggtacaagtg aacgatgagc aaagctttgt cgaacaagct gaatggagca tgttagttca
     8221 gctgttcaat caacgtctgc aggaacgcat acagtcgggc gagttgaaga ctatttctgg
     8281 cggcactgcg cgcagcgtta aaattgcacc cgattatcag tcgctgtttt tccgtgtaaa
     8341 tgcacgagac gataatatgc aggatgctgc gaatgcatta atggcagagt tggcaaccat
     8401 tgatcagcat ggtttttctg ctgaagaact cgatgatgtc aaatctaccc gcctcacctg
     8461 gctgaaaaat gcggttgatc agcaagctga acgtgattta cgtatgctga ccagtcgcct
     8521 ggcatccagc tcattaaata atacgccgtt cttgtcgccg gaagagacat atcaactttc
     8581 gaaacgtctg tggcagcaaa ttaccgtgca aagtctggcg gaaaaatggc agcagttaag
     8641 aaagaaccag gacgcatttt gggagcaaat ggtaaacaat gagcttgccg ccaaaaaagc
     8701 attgtctcct gcggctatcc tggcgctgga aaaagagtac gccaacaaaa agctggcggc
     8761 ttacatcttc ccaggcagaa atttatcgtt aacagtagac gctgacccac aggcggaaat
     8821 tagcagcaaa gaaacgctgg ctgagaatct gacatcatta acactttcca atggtgccaa
     8881 ggttattctg gcaaaatccg cgggtgaaga gcaaaagcta caaattattg ccgtatcgaa
     8941 taaaggcgat ttaagtttcc ctgcgcagca aaaatcactt atcgcgctgg caaataaagc
     9001 agttagcgga agcggcgttg gcgaactctc ctcttccagc ctgaaacgct ggagtgcgga
     9061 aaactcggta accatgagca gtaaagtcag tggcatgaat acgttgctct ccgttagcgc
     9121 gcggactaat aaccctgaac ctggtttcca gttgattaac cagcgaatca cccacagcac
     9181 gattaacgac aatatttggg catcgctaca aaatgctcaa attcaggcgt tgaaaacgct
     9241 cgaccagcgt ccagcggaga aattcgccca gctgatgtat gagacgcgct atgctgatga
     9301 ccgcacgaaa ttactgcaag aaaatcagat tgcacagttt actgccgcag atgcgctggc
     9361 tgccgatcgc cagttgtttt catctccagc ggatatcacg tttgtcattg tcggtaatgt
     9421 cgcagaagac aaactcgtgg cgttaattac gcgttactta ggctcaatca aacactctga
     9481 ttcgccatta gccgcaggta aaccattaac tcgcgcgacg gacaacgcat cggttactgt
     9541 aaaagaacaa aatgaacctg tggcacaggt ttcacagtgg aagcgttatg attcccggac
     9601 acctgttaat ctggcgacgc gtatggcgct cgatgctttt aacgtcgcac tggcaaaaga
     9661 tctacgcgtt aatattcgtg aacaggcatc tggggcatac agcgtttctt ctcgcctctc
     9721 ggttgatcct caggctaaag atatcagtca tttgctggct tttacttgtc aaccagaacg
     9781 acatgatgaa cagttaacgt tagcgaatga agtgatggtt aagcgtttga ctaaggggat
     9841 cagtgagcaa gaactgaacg aataccagca aaacgttcag cgcagcctcg atatccaaca
     9901 gcgtagcgtt caacaattag cgaacactat tataaatagt cttattcaat atgacgatcc
     9961 tgcagcatgg actgagcagg agcaattgtt gaaacaaatg acggtagaga atgttaacac
    10021 tgccgttaaa caatatcttt ctcatccggt aaatacttat accggagtat tattgccaaa
    10081 ataataacag ccccgtcaac acatcgttgg cggggatttt agcaatattc gctattttta
    10141 tgtaataatt ttataaatgc gttcaaaata ataaccaagt actaatagtg atattttaat
    10201 gcctgatttt tacgtgataa ttcaggaggc acagaaagcg cataaaaata acaacataaa
    10261 acacctttcc accacccaag aattctctat tacattgttt ttaatgaaaa aatatcattc
    10321 caacaatatc tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca
    10381 cttgcttact ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt
    10441 taaaatggat aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg
    10501 tgcgaagtct atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga
    10561 cgatgtcgca tttcagatta tcaatgatga attatatctt gatggcaacg ctcgtcagaa
    10621 cctggccact ttctgccaga cctgggacga cgaaaacgtc cacaagttga tggatttatc
    10681 cattaacaaa aactggatcg acaaagaaga atatccgcaa tccgcagcca tcgacctgcg
    10741 ttgcgtaaac atggttgccg atctgtggca tgcgcctgcg ccgaaaaatg gtcaggccgt
    10801 tggcaccaac accattggtt cttccgaggc ctgtatgctc ggcgggatgg cgatgaaatg
    10861 gcgttggcgc aagcgtatgg aagctgcagg caaaccaacg gataaaccaa acctggtgtg
    10921 cggtccggtg caaatctgct ggcataaatt cgcccgctac tgggatgtgg agctgcgtga
    10981 gatccctatg cgccccggtc agttgtttat ggacccgaaa cgcatgattg aagcctgcga
    11041 cgaaaatacc atcggcgtgg tgccgacttt cggcgtgacc tacaccggta actatgagtt
    11101 cccgcaaccg ctgcacgatg cactggataa attccaggcc gacaccggta tcgacatcga
    11161 catgcacatc gacgccgcca gcggtggctt tctggcaccg ttcgtcgccc cggatatcgt
    11221 ctgggacttc cgcctgccgc gtgtgaaatc gatcagtgct tcaggccata aattcggtct
    11281 ggctccgctg ggctgcggct gggttatctg gcgtgacgaa gaagcgctgc cgcaggaact
    11341 ggtgttcaac gttgactacc tcggtggtca gattggtact tttgccatca acttctcccg
    11401 cccggcgggt caggtgattg cacagtacta tgaattcctg cgccttggtc gtgaaggcta
    11461 taccaaagta cagaatgctt cctaccaggt cgctgcctat ctggcggatg agatcgccaa
    11521 actggggccg tatgagttca tctgtaccgg tcgcccggac gaaggcatcc cggcggtttg
    11581 cttcaaactg aaagatggtg aagatccggg atacaccctg tacgacctct ctgaacgtct
    11641 gcgtctgcgc ggctggcagg ttccggcctt cactctcggc ggtgaagcca ccgacatcgt
    11701 ggtgatgcgc attatgtgtc gtcgcggctt cgaaatggac tttgctgaac tgttgctgga
    11761 agactacaaa gcctccctga aatatctcag cgatcacccg aaactgcagg gtattgccca
    11821 gcagaacagc tttaaacata cctgataacg tttaacggta acggtgtccc gaaacgaacc
    11881 cgtttcggga taatttccaa agtctgttca ctggcattag caacggaaaa tattgttctg
    11941 aatacgcttc agaacaaaac aggtgcggtt ccgacaggaa taccgtttta gggggataat
    12001 atggctacat cagtacagac aggtaaagct aagcagctca cattactcgg attctttgcc
    12061 ataacggcat cgatggtaat ggctgtttat gaatacccta ccttcgcaac atcgggcttt
    12121 tcattagtct tcttcctgct attaggcggg attttatggt ttattcccgt gggactttgt
    12181 gctgcggaaa tggccaccgt cgacggctgg gaagaaggtg gtgtcttcgc ctgggtatca
    12241 aatactctgg gtccgagatg gggatttgca gcgatctcat ttggctatct gcaaatcgcc
    12301 attggtttta ttccgatgct ctatttcgtg ttaggggcac tctcctacat cctgaaatgg
    12361 ccagcgctga atgaagaccc cattaccaaa actattgcag cactcatcat tctttgggcg
    12421 ctggcattaa cgcagtttgg tggcacgaaa tacacggcgc gaattgctaa agttggcttc
    12481 ttcgccggta tcctgttacc tgcatttatt ttgatcgcat tagcggctat ttacctgcac
    12541 tccggtgccc ccgttgctat cgaaatggat tcgaagacct tcttccctga cttctctaaa
    12601 gtgggcacac tggttgtatt tgttgccttc attttgagtt atatgggcgt agaagcttcc
    12661 gcaactcacg tcaatgaaat gagcaacccc gggcgcgact atccactggc tatgttactg
    12721 ctgatggtgg cggcaatctg cttaagctct gttggcggtt tgtctattgc gatggtcatt
    12781 ccgggtaatg aaatcaacct ctccgcaggg gtaatgcaaa cctttaccgt tctgatgtcc
    12841 catgtggcac cggaaattga gtggacggtt cgcgtgatct ccgcactgct gttgctgggt
    12901 gttctggcgg aaatcgcctc ctggattgtt ggtccttctc gcgggatgta tgtcacagcg
    12961 cagaaaaacc tgctgccagc ggcattcgct aaaatgaaca aaaatggcgt accggtaacg
    13021 ctggtcattt cgcagctggt gattacttct atcgcgttga tcatcctcac caataccggt
    13081 ggcggtaaca acatgtcctt cctgatcgca ctggcgctga cggtggtgat ttatctgtgt
    13141 gcttatttca tgctgtttat tggctacatt gtgttggttc ttaaacatcc tgacttaaaa
    13201 cgcacattta atatccctgg tggtaaaggg gtgaaactgg tcgtggcaat tgtcggtctg
    13261 ctgacttcaa ttatggcgtt tattgtttcc ttcctgccgc cggataacat ccagggtgat
    13321 tctaccgata tgtatgttga attactggtt gttagtttcc tggtggtact tgccctgccc
    13381 tttattctct atgctgttca tgatcgtaaa ggcaaagcga ataccggcgt cactctggag
    13441 ccaatcaaca gtcagaacgc accaaaaggt cacttcttcc tgcacccgcg tgcacgttca
    13501 ccacactata ttgtgatgaa tgacaagaaa cactaaccat gtctgaatgg ttataagaca
    13561 agggagcgat aattcatcgc tccctttttc gtgcttgctg tcgtattgac ttaaccggga
    13621 taagtacgag aatgagcgca catctgttta ccggaaacca gcacatatgg atatctgctc
    13681 ccgaaacaag aaattaacga ttagaagacc agcgatactg gttgcattgg cacttttact
    13741 gtgtagttgt aaaagcacgc ctccagagtc catggtgaca ccaccagcag gttcaaagcc
    13801 accagccacg acgcaacaat cgtcacaacc gatgcgtggc atctggctgg ccacggtttc
    13861 tcgcctcgac tggccaccgg tttcctcggt taacattagt aatcccacca gccgagcccg
    13921 tgtacaacaa caggcgatga tcgacaaact ggatcatctc caacgtctcg gcataaacac
    13981 ggtctttttc caggtcaagc cggacggtac cgccctgtgg ccatcgaaaa ttttgccgtg
    14041 gtccgatctt atgaccggta agattggtga aaatccgggt tacgatccgc tgcaattcat
    14101 gctcgacgaa gcccacaagc gtgggatgaa agtacacgcc tggtttaacc cctatcgcgt
    14161 atcggttaat acgaagcccg gtactatcag ggaactgaat agcactctgt ctcaacaacc
    14221 ggcgagcgtc tatgtgcaac accgtgactg gatcagaacg tctggcgatc gctttgtcct
    14281 cgacccgggc atccctgagg ttcaggactg gatcacatca atagtcgctg aagtggtttc
    14341 ccgctatccg gtagatggcg tgcagtttga cgactatttc tatactgaat caccgggttc
    14401 acggctaaat gataacgaaa cgtaccgtaa atacggaggc gcatttgcgt caaaagcaga
    14461 ctggcggcgc aacaatactc agcagttaat tgcaaaggta tcacacacca ttaaaagcat
    14521 taagccggga gtcgaatttg gcgttagccc ggcaggcgtg tggcgtaacc gatcacacga
    14581 tccgctcggt tccgataccc gaggcgcggc agcctatgac gaatcctacg cagacacccg
    14641 tcgatgggtg gaacaaggat tgctggatta cattgctccc caaatttact ggccgttctc
    14701 acggagtgcc gcgcgttatg acgtgttggc aaaatggtgg gcggatgtcg ttaaaccaac
    14761 caggacccgc ctgtatatcg gtatcgcctt ctataaagtg ggtgaacctt caaagataga
    14821 gccagactgg atgattaacg gcggcgtacc ggaactgaaa aagcagctcg atcttaacga
    14881 tgcggtacca gaaattagcg gcaccatctt gttccgtgag gactatctga ataaaccgca
    14941 gactcaacaa gcggtcagct atctgcaaag tcgttggggc agttaaacta ccatgcttac
    15001 tttgtaacaa gccggtgctt tacccaccgg cttgttacac cttgtgaaat atcccactag
    15061 taatcatgct tacataagtc aaattaacca cgcaattaac cacacttagt taaacagcta
    15121 taaatcccta ataaaaatgt atgaatatga aaaaccatac gatattcgct gtcaccgaaa
    15181 aataattacg atgggctata ctcttctgaa gaacagtagc gtagattgaa actgtcttaa
    15241 gtcgcttatt tcttttcagt atatcttcat atttcaggag aatatggtgc agttagtcga
    15301 tctcgcacgt tgcgtttcgt tttgattccg tctgatttcc cagccttata agggtgtgaa
    15361 ttatggagat gtattttaaa agaatgaaag atgagtggac cggacttgtc gaacaggcag
    15421 atcctctcat tcgtgctaaa gccgcagaaa ttgcccttgc gcatgctcat tatctgagta
    15481 tcgagtttta tcgaattgtc cgcatcgacc cgcatgccga agaattcttg agtaatgaac
    15541 aagttgagcg gcagttgaaa agtgcgatgg aacgctggat tattaacgtg ctttctgccc
    15601 aggttgacga tgtcgaaagg ctaatacaaa tccagcatac cgtcgcggaa gtgcatgccc
    15661 gcataggaat tccggtagaa attgtcgaga tggggtttcg ggtgctgaaa aaaatcctct
    15721 atccggtaat cttctcttcg gattattccg ccgcagaaaa acttcaggtc taccatttct
    15781 cgattaacag tattgatatc gcgatggaag tgatgacccg cgcgtttacc tttagtgaca
    15841 gtagtgcctc aaaggaagat gaaaactatc gtatcttctc gttactggaa aacgccgaag
    15901 aagaaaaaga acggcaaata gcctcaatac tttcatggga aatagatatt atctataaag
    15961 tcctgctgga ttctgattta ggcagcagtt tgcctttaag ccaggctgat tttggcctgt
    16021 ggtttaacca taaaggtcga cattatttta gcggcattgc cgaagtgggc catatctccc
    16081 gtctgattca ggatttcgac ggtattttca atcaaaccat gcgtaacacc aggattttga
    16141 ataacagaag tttgcgggta aaatttctat tacagataag aaataccgta tcgcagatta
    16201 ttaccttgct gcgtgaattg tttgaagaag tatcgcgcca cgaagtcggt atggatgtac
    16261 tgacgaaatt acttaaccgc cgtttcctgc cgactatctt caaacgcgaa attgcccatg
    16321 ccaaccggac cggtacaccg ctgtcagtgc tgattattga cgttgataaa ttcaaagaga
    16381 tcaacgatac gtggggccat aacactggcg atgaaattct gcgtaaagtc tcattcctct
    16441 cgcagaagag attggtgaaa tcgaaaatat tgggcgctgg gtcatcgcgg aagcttgccg
    16501 tcagttagca gaatggcgta gccagaatat tcatatcccg gcgttatccg tgaacttgtc
    16561 ggcgctgcac tttcgcagta atcagctgcc taatcaggtg tctgatgcaa tgcaagcctg
    16621 gggtattgac ggccaccagc tgacggtgga aatcacggaa agcatgatga tggaacacga
    16681 taccgaaatc tttaagcgca ttcagatcct gcgtgatatg ggcgtgggtt tatcggtaga
    16741 tgattttggc acgggctttt ccggattatc ccgcttagtc agtcttccgg taacggaaat
    16801 caaaattgac aaaagctttg tcgatcgttg tctgacggaa aaacgcatcc tggccttact
    16861 tgaagccatt accagcattg ggcaaagcct caatttaacc gtcgtggcgg aaggggtcga
    16921 aaccaaagag caatttgaga tgctacgtaa gatccactgt cgcgttattc agggatattt
    16981 cttttcccgc ccccttcccg ccgaagaaat tccaggctgg atgagcagcg tgttaccgct
    17041 gaaaatctga caaattcctc tcgcccgcac tcgcgggttt tcctttaagg tgacactgtc
    17101 acttaaatac tgtgatttca gctacgattc cgggagattc cttccttaac tttccctctt
    17161 gcaccaaaac tgcactacgc tgcacaaatg ttgatcaaaa gttaaaaaat ataactttaa
    17221 gccagcattc tgccgcttat cccggcatgg catgagatct gcataagcgg aaagcgcagc
    17281 aatttttgtc ttatacagga accgtttatg tcggatacca ccgaactggt tgatttagcc
    17341 gtgatcttcc ctgatctgga gatcgaattg aaatatgcct gcgttgataa catcacaggt
    17401 aaagctattt atcagcaagc gcgttgtctg ctacataaag atgcgattac cgcgctggcg
    17461 aaaagtatca gtatcgccca gctgtcaggc ttacaactgg tgatttacga tgcgtatcgc
    17521 ccacaacaag cacaggcgat gttgtggcaa gcctgcccag acccgcaata tgttgttgat
    17581 gtgacggtcg gttctaatca cagccgtggc acggcgctcg acctgacgct tcgtgatgac
    17641 gtggaggtgg tgaatgatgc taagcgagga aacgtccgcc gtacgcccac aaaaacaaac
    17701 gcgatttaac ggtgcaaaac tggtatggat gctgaaaggc agtccgctca ccgtgaccgg
    17761 cgcagtcatc attgtattaa tgcgactgat gatgattttt tcaccgtggc tggcgacgca
    17821 tgatcccaac gccattgatt taaccgcccg ccttttgccg ccttctgcgg cgcactggtt
    17881 tggcaccgat gaagtgggac gcgatctgtt tagccgcgta ctggtcggca gtcagcaatc
    17941 aattctcgcc ggattagtgg tggtcgccat tgcgggtatg attggttcgc tactcggatg
    18001 tctatccggt gtgcttggcg gacgcgcaga cgccatcatc atgcgcatca tggacattat
    18061 gctgtcgatt ccttcgttgg tactgacaat ggcactggct gccgctctcg ggccgagttt
    18121 atttaacgcc atgctggcga ttgctattgt gcgaattccc ttttatgtgc gcttggcgcg
    18181 ggggcaagca ttagttgtac gccagtatac ctatgttcag gcggcgaaaa cctttggcgc
    18241 gtctcgttgg catctgatca actggcatat tttacgtaac tccctaccgc cgctgatcgt
    18301 gcaggcatcg ctggatatcg gtagcgcgat tttaatggcc gccacgttgg gatttattgg
    18361 cctaggtgct caacaaccga gtgctgaatg gggcgcgatg gtagcgaatg gtcgcaacta
    18421 tgtactcgat cagtggtggt attgcgcatt tccgggggca gcgattttgc ttacctccgt
    18481 cgggtttaat ctctttggtg atggtattcg cgatctgctt gacccgaaag caggaggaaa
    18541 gcagtcatga cccaacccgt tctggacatt caacagctgc atttgagttt ccccggtttt
    18601 aacggtgatg ttcacgcgct caacaatgtg tccttgcaga ttaaccgcgg cgaaattgtc
    18661 ggtctggtgg gagaatccgg ctcaggtaaa tcagtcaccg caatgctgat tatgcgtctg
    18721 ctaccgacgg gcagttattg cgtacatcgg gggcagattt cactgctggg agaagatgtt
    18781 cttaacgctc gggaaaaaca gcttcgtcag tggcgcggcg cacgagtggc gatgatcttt
    18841 caggaaccga tgaccgccct taatccgaca cgtcgaatag gtcttcagat gatggacgta
    18901 atccgccatc atcaaccaat aagtcgtcgg gaagccagag ctaaagcgat tgccctgctg
    18961 gaagagatgc aaatcccgga tgccgtggaa gttatgtcgc gctatccgtt tgagctttca
    19021 ggtggtatgc gccagcgggt aatgattgcg ctggcattct cctgcgagcc gcaattgatt
    19081 attgccggcg aaccgacgac ggcgctggac gtcacagtac agttgcaggt actgcgtttg
    19141 cttaaacata aagcacgcgc cagtggaact gcggtactgt tcatcagcca tgatatggcc
    19201 gtggtgtcgc aactgtgcga tagcgtttac gtgatgtatg ccggaagcgt aattgaaagc
    19261 ggcgggacgg cagacgttat ccatcatccc cggcatccgt ataccattgg tttgctgcaa
    19321 tgcgcaccag aacatggagt accacgccag ccattacccg ccattccagg gacggtacca
    19381 aacctcaccc atttgcctga cggctgcgct tttcgcgatc gttgctatgc ggcaggtgca
    19441 cagtgtgaaa acgtcccggc gctgacagcg tgtggtgaca acaaccagca ctgcgcctgt
    19501 tggtatccgc agcaggaggt cataagtgtc tgacacgtta ttaacgttac gcgacgtcca
    19561 tatcaatttt ccggcccgta aaaactggct tggtaaatct acggaacatg ttcatgccat
    19621 taatggtatt gatttacaga tccgccgtgg tgaaacctta gggatcgtcg gcgagtcagg
    19681 ctgcggcaaa agcaccctcg cacagctttt aatgggcatg ctgcaaccga gccacgggca
    19741 gtacatccgt tcaggctcac aacgcattat gcagatggtg tttcaggacc cgctctcttc
    19801 gcttaatccg cgcttaccgg tgtggcgcat catcacagaa tcgctctgga tagctaagcg
    19861 tagtagtgaa caacagcggc gagcgttggc agaggagttg gctgtgcagg tgggtattcg
    19921 tccggagtat ctcgaccgcc tgcctcatgc gttctccggc gggcagcggc aacgcatcgc
    19981 cattgccaga gcactctctt cgcagcctga cgtgattgtg cttgatgagc caacctcggc
    20041 gctggatatc tccgtgcagg cgcagatcct caatttgctg gtaacgctac aggaaaatca
    20101 cgggctgact tatgtgctga tttcacacaa tgtctcggtg atacgtcata tgagcgatcg
    20161 ggtggcggtg atgtatctcg ggcagattgt agaactgggc gacgcgcaac aggtgttgac
    20221 tgcttcggca catccataca cccgattatt gctggattcc ctccccgcca ttgataaacc
    20281 gctggaggaa gaatgagcat tacgtaaaac ggatctgccg ggaaaccgca cgttgccgca
    20341 aggctgtttt ttctacgaac gttgccctct ggcaacccac ggatgtgaag tccggcaatc
    20401 attagcaata agggaggacg gacgtgagct ccggtgctgg cgggcgctgt agagatgcta
    20461 acaacgcatc aggcattcac gcgcctgacg cgtcatccgg caatgcttta cgatttcaac
    20521 tggtaatcca gcgtaatttc cgctttcagt acctgagaga ccgggcatcc tgcttttgct
    20581 ttctggatga tgccgtcaaa ggtagaggca tcaatacccg gcaccgcaac ttcactcttc
    20641 agtgcgattt tcgtaatcgc aaaaccggca tccactttat ccagcgacac atcggcggtg
    20701 gtatcaattg atgttggcgt gaatcccgct tcccccagca ttaatgaaag cgccattgag
    20761 aaacatgcgg catgcgctgc gccaatcagt tcttcagggt tggttccttt ttcgccttca
    20821 aaacgcgtgt taaatccata cggctgttgg ttcagcacgc cgctctcggt ggatactgtt
    20881 cccttcccgc gtttgatatc gccttcccag tgtgcctgac ctttcttatg gattgtcatt
    20941 gttgctctcc tgtgggctta aaaaggaaat tatagactac gtgagaaaca gcacgaggat
    21001 aagcaggata ttccgaataa aatacggcga aaatattact tctgcactta aattaaattc
    21061 ccacaataga taaccgggaa tatatatctc aaga
//
LOCUS       DAEEGA010000069        20758 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEEGA010000069 DAEEGA010000000
VERSION     DAEEGA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20758
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.069"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..564
                     /locus_tag="KLH46_002783"
     CDS             <1..564
                     /locus_tag="KLH46_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE0379405.1"
                     /translation="AALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAMASQRA
                     KEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHAQ
                     KTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFS
                     HYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            582..1220
                     /locus_tag="KLH46_002784"
     CDS             582..1220
                     /locus_tag="KLH46_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE0379406.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1511..2239
                     /gene="mpaA"
                     /locus_tag="KLH46_002785"
     CDS             1511..2239
                     /gene="mpaA"
                     /locus_tag="KLH46_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBE0379407.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2214..3173)
                     /gene="ycjG"
                     /locus_tag="KLH46_002786"
     CDS             complement(2214..3173)
                     /gene="ycjG"
                     /locus_tag="KLH46_002786"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBE0379408.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3292..3798
                     /gene="tpx"
                     /locus_tag="KLH46_002787"
     CDS             3292..3798
                     /gene="tpx"
                     /locus_tag="KLH46_002787"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBE0379409.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3842..5383)
                     /gene="tyrR"
                     /locus_tag="KLH46_002788"
     CDS             complement(3842..5383)
                     /gene="tyrR"
                     /locus_tag="KLH46_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBE0379410.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5531..6592)
                     /locus_tag="KLH46_002789"
     CDS             complement(5531..6592)
                     /locus_tag="KLH46_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBE0379411.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6589..7986)
                     /locus_tag="KLH46_002790"
     CDS             complement(6589..7986)
                     /locus_tag="KLH46_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBE0379412.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8142..9140
                     /locus_tag="KLH46_002791"
     CDS             8142..9140
                     /locus_tag="KLH46_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE0379413.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADKSFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9251..10123)
                     /gene="ompG"
                     /locus_tag="KLH46_002792"
                     /pseudo
     CDS             complement(9251..10123)
                     /gene="ompG"
                     /locus_tag="KLH46_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10196..11277)
                     /locus_tag="KLH46_002793"
                     /pseudo
     CDS             complement(10196..11277)
                     /locus_tag="KLH46_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11291..11950)
                     /gene="pgmB"
                     /locus_tag="KLH46_002794"
     CDS             complement(11291..11950)
                     /gene="pgmB"
                     /locus_tag="KLH46_002794"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBE0379414.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11947..14214)
                     /gene="ycjT"
                     /locus_tag="KLH46_002795"
     CDS             complement(11947..14214)
                     /gene="ycjT"
                     /locus_tag="KLH46_002795"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBE0379415.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14211..14834)
                     /locus_tag="KLH46_002796"
     CDS             complement(14211..14834)
                     /locus_tag="KLH46_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE0379416.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14831..15253)
                     /locus_tag="KLH46_002797"
     CDS             complement(14831..15253)
                     /locus_tag="KLH46_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE0379417.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15275..16050)
                     /locus_tag="KLH46_002798"
                     /pseudo
     CDS             complement(15275..16050)
                     /locus_tag="KLH46_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16068..17120)
                     /locus_tag="KLH46_002799"
     CDS             complement(16068..17120)
                     /locus_tag="KLH46_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBE0379418.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17151..17993)
                     /locus_tag="KLH46_002800"
     CDS             complement(17151..17993)
                     /locus_tag="KLH46_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBE0379419.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(17980..18861)
                     /locus_tag="KLH46_002801"
     CDS             complement(17980..18861)
                     /locus_tag="KLH46_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE0379420.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18882..20165)
                     /locus_tag="KLH46_002802"
                     /pseudo
     CDS             complement(18882..20165)
                     /locus_tag="KLH46_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20179..>20758)
                     /gene="ycjM"
                     /locus_tag="KLH46_002803"
     CDS             complement(20179..>20758)
                     /gene="ycjM"
                     /locus_tag="KLH46_002803"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HBE0379421.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5065 a         5659 c         5049 g         4985 t
ORIGIN      
        1 gccgcgctga cagaaaaacg aattaaagct gtagtgagta ttacgggtgt taatatcggg
       61 cgtctttttc gggaaggttt cagcaattat gatccgattg gcgtattgaa tgcaatggcg
      121 tcacaacgag caaaagaggc tcgtggtgga gaattacaga tcaacgaatt acttcccgcc
      181 agcctggatg cagcaaaagc ccatggactg accgaacgcg atgtttatga agcaactgac
      241 tactataaaa caccacgcgg gcaacaaccg ggaggtgcga caaaaatgtt gttctcacat
      301 gcacagaaaa cgctggcctg ggatgccttc gcatttactg aagtgttact gacccagccg
      361 gtgatggtag tagttggtga aaaagtcggt gccttcggtg cttatcgtga tggactggaa
      421 gtctatggcc gggcgatggt ttcgcaggat cgtcagttag tttcgttacc cgatttctca
      481 cattatgaac tatatgacaa accggaagca gttcaggagg cgctggcaaa agttattcct
      541 tttttcaaca cccatctggg ataagtttca tgagaaatat tatgaaaaat gtactgattc
      601 ttggtgctgg cggtcagata gcccgccatg tgattaatca actggcagat aaacaaacta
      661 ttaaacaaac tttatttgcc cgtcagccag caaaaataca caaaccctat ccaactaata
      721 gccagattat tatgggggat gtactgaatc atgcagcact gaagcaggcc atgcagggac
      781 aggatattgt gtatgccaac cttacggggg aagatttaga tattcaggca aattcagtga
      841 tcgctgcgat gaaagcctgt gatgttaaac gtctgatctt tgttctttcc ctcggtattt
      901 atgatgaagt tccaggaaaa tttgtcgaat ggaataatgc agttattggt gaacccctaa
      961 aaccattccg cagggctgcg gatgcaattg aggcttctgg actggaatat acaattcttc
     1021 gccctgcctg gctgactgat gaagatatta ttgattatga gcttacctct cgcaatgaac
     1081 cattcaaagg tacgatcgta tcacgtaaga gtgtcgcagc attaattaca gatatcatcg
     1141 ataaacctga gaaacacatt ggtgagaata taggcatcaa tcaacccggg actgatggcg
     1201 ataagccatt cttcatgtaa atcaattata tagataccat cgtactgtac tatgtgcagt
     1261 acgatttatt aaacttatta aggtgaactg atcaatcaga tgcgattaac atgtgtagaa
     1321 ccagtaagaa taaaatacgc catgtatata acattacgac aattctaata tctgcacatc
     1381 aatgaaaatg caaacaactt ccggcaaaaa agtgatacaa gatcgatgac atagcgttga
     1441 agaaaaggat ttgataatcc gcaaaatttg cacaacactg cctgtaatac ttcctgaacc
     1501 agagacgatc atgaccgtaa cccgctcacg cgccgaacgc ggcgcatttc cgcccggaac
     1561 agaacattac ggacgttcat tattgggcgc gccgttaatc tggtttcctg cccctgcagc
     1621 cagccgcgaa agtggtttga ttctggctgg cactcacggt gatgaaaact cttcggtcgt
     1681 cacgctctcc tgtgctctgc ggactttgac accttctttg cgccgccatc atgtggtgct
     1741 gtgtgtgaat cctgacggct gccagctggg gttacgggcc aatgctaatg gtgtggattt
     1801 aaacagaaac tttccggcgg cgaactggaa ggaaggtgaa acggtttatc gctggaacag
     1861 cgccgctgaa gaacgtgatg tcgtattact gacaggtgac aaacctggct ccgaacctga
     1921 aacccaggcg ctgtgccagc ttatacaccg cattcaacct gcctgggtgg tctcattcca
     1981 cgatccactg gcttgtattg aagatcccag acacagcgaa ttaggcgaat ggctggccca
     2041 gtcgtttgaa ttgcctctgg taaccagcgt cggttatgaa acgcctggct ctttcggcag
     2101 ttggtgtgca gatctcaact tgcattgcat caccgcagag tttccgccca tctcctccga
     2161 cgaagccagc gaaaaatatc tctttgcgat ggcaaacttg ctgcgctggc atcctaaaga
     2221 tgcaattcgc ccgtcgtgaa ctgaagcgcc ggttccacat ctaccgccag ccaggtcggt
     2281 ccgtcaagat cggcgaaact gacctgcggc accagcggta aagcggcgct aatggcacga
     2341 gaggtacaca acatgcagcc cagcatcaga ctgaaacctt gtgcacgcgc ttcagtcgcc
     2401 agcgcttccg tcagaccacc ggttttatcg agcttaatgt taaccatctc atagcgccct
     2461 ttcagcgcct tcagattgct acgagtatga caactttcat cagcacaaat cggcaacgga
     2521 tgaataaaat tctccagcgc cgcatcgtcc tgcgcaggaa gcggttgttc aagcatcgca
     2581 acgcctaaat ccgccaatag ctggcaacgc gccgccaacc cttctgcacg ccaggattca
     2641 tttgcatcaa cgatcagcgt ggcatcgggc acagcggtgc gaattgccac catccgctca
     2701 ctgataagat ggttatccag cttcactttc agtaatttcg cgcctgcctg ccagagtgtt
     2761 gatgcactat tggccatctg atcaggcgta ccgatgacaa ccgtctgtgc agtgataact
     2821 gtctcgggaa gcgttatgcc gatcaaatca gccagcgatt gctgctgttt tcgcgccgcg
     2881 agatcccaca atgcacaatc cagcgcatta cgtgctgcgc cggcagggag aattttttgc
     2941 aactcctccc gtgtcagccc tttctctaat tgcggcacga cgctcataat ttgcgccatt
     3001 accgaggcat cactttctcc ataacgcgga tacggcgtgc attcgccggt gcctttaata
     3061 ccctcttctt ccagttcaac caccaccacg cgcgcttcac tgcgacttcc ccgggcaatc
     3121 acaaacgggg tatgtaaggg ccaggcttcc tcaaatacct taacgattct catctttact
     3181 ccttgttgtc gcggtggtct gcggattaag cgcaaatagc gtttgctgtg ttattgacag
     3241 ttagcataaa ctaggtgtga cgttaactat atgtaaacag gaaagataat catgtcacaa
     3301 accgttcatt tccagggcaa cccggttaca gtcgccaatt ccatcccgca ggcgggtagc
     3361 aaagcgcaga cttttactct cgtggcaaaa gatctgtctg acgtcaccct cggtcagttt
     3421 gcgggtaaac gcaaagtgct gaacattttc ccgagtattg ataccggtgt ttgcgccgca
     3481 tcagtacgta agtttaacca actggcaact gagatcgaca acaccgttgt gctgtgtatc
     3541 tctgccgatc tgccgttcgc ccagtctcgt ttctgcggcg cagaaggtct gaacaacgtt
     3601 atcaccctct ccactttccg taacgctgaa tttctgcaag cctacggtgt ggcaattgct
     3661 gatggcccac tgaaaggtct ggcagcgcgt gccgttgtgg ttattgacga aaatgacaat
     3721 gtgattttca gccagctggt ggatgaaatc accaccgagc cggattacga agcagctctg
     3781 gctgtactga aagcataagt taatatgcct gatggtgttg caccatcagg catattcgcg
     3841 cttactcttc gttcttcttc tgactcagac catattcccg caacttattg gcaatcgcgg
     3901 tatgtgaaac gccgagacgt tttgccagtt tgcgcgtgct gggataattg cgataaagct
     3961 gggttaatac cgagcgttca aaacggctgg tgatttcgtc cagcgaacct tccatcgcat
     4021 cttcgcccac ggctaccgtt gcggcgtcat aatccggcaa caaaatatcc tgtggacgca
     4081 gctcataacc gtccagttgt gtcagtgcgc gatagatagc gttctttaac tgccgcacat
     4141 ttcccggcca cgcataacgt gtaagtacag tattcaggtc agcggccagt ttcggacgcg
     4201 gcacgccctg ctcgtcggca aagcgggcga cgaacagctc agttaacggc ataatgtcct
     4261 gcggacagtc acgtagcggc ggcagattga gcgtcaacac gttcagacga taatagagat
     4321 cttcacggaa cacgcctttt tgcaccagtt cgaccagatt cttctgcgta gcgcaaatca
     4381 cccgcacatc gacatgcacc tcatggtctt cgccaacccg acggaaagtg ccatcattaa
     4441 gaaaacgcag taatttcgcc tgcatccgtg gtgacatttc ccctatttca tccaacagca
     4501 ccgaaccacc gttcgcctgc tcaaagaacc ctttcttccc ttccggagca tgaccaaaca
     4561 gttcactctc gaccgcatct tctggtatag acgcgcaatt cagcgccagg taaggtttgc
     4621 ccgctctggg gcttgcctga tggcaggcgt aagcaaaaag atctttacct gtacctgtgt
     4681 cacccgtaat cagcagcggc gcgcttagca tcgccagttt ctgcgcctgt tcgacaacgt
     4741 gcttcatttt cgggctgacg gcgacaattt ggctgaaggc gctgacgtcc tgggcggcga
     4801 cattttgcaa ctggcggccc atacgaatcg ttgatcgcaa catcaccacc gcaccggtca
     4861 ggacgtgttg atcattttca tcctgaagat aaacaggcgt aatctccatc aggaaattct
     4921 gcccattaat aacgacatgc tcgttatgcg aatcttgcgg ttcgctttcc agccaacgta
     4981 aaaaattaaa gccgttaatc aattgtgcgg cggtatggtt gcgcaggcga tccaattttt
     5041 gcccaaaaag ctgacagctc gccgggttcg ccatatccac tttgcttttc atatcgacag
     5101 agagcacagg ttcaggcaac gcctccagta acgcgctcaa cgccagatgc tcacgttcgg
     5161 aaggcatcca cgggacagta cgcacatcgg taacacctgc aatacggcgt atttcggcca
     5221 tcagactgct gaaactctca aactccagtt cagcaaaatt gaggtagatt cgcccaatgg
     5281 gatcaatctc aataccgcgt aaatcaatgc ctcttagcac gagtagatcg agtaattcgc
     5341 gggtcagacc gagtcggtct tcacaaaaga cttccagacg catgggaacc ttcacctgaa
     5401 aaaagaacaa ttaatatgat gatatgacac tatttggata gcaggaaggt ttctgtcaac
     5461 aatcattgac acaaagacgg aaaaacgggc acttcggcgt aaagatatcc ggtcagcacc
     5521 cgatattgca ttatttttcg ctgggcgttt tgcctttttg cagagtttct ttcacctgac
     5581 cgataagctg acgacggaaa tccccaaggc gaggtttgtc atcgtcaatc cacggcagcg
     5641 ggcggcaaag ctccatagct ttaatcccga gtcgtaccgt cagaagtcca gcaccaattc
     5701 cctgagctgc gcgggtagac aaacgagcag cgagatcttg cgacatccag tccatcccca
     5761 cttcgcgcac cagttcgctg gctccggcaa aagcgatatt cagcaatacc agcttaaaca
     5821 gacgcaaacg gctgtaatac cccagttcaa tgccatacag cgtggcaatg cgattaatta
     5881 aacgcagatt gcgccaggcg ataaacgcca tatcgaccaa cgccagcggg ctgaccgcaa
     5941 tcatcaacgt tgattccgcc gccgaatggc tgatttcgcg ccgcgcctgg gcatctaaaa
     6001 ctggctggac caaatgcgca tacaggctga ccacttcacg atcgttctgc gtttcatgga
     6061 ttgaggcata ccagcgttgc agcgccggat gcgactgatc aatacccgcc tgctgcgcca
     6121 gtttttcgca aaatgcgcga cctttgcccg tgccgtggct atgcaataaa tcacgcgctt
     6181 cgtcgcgttc atgggcgcgc tgtcgcaagc gccataagcg ccgccactct gttaccacag
     6241 aacctacgcc agcgccgata atcaatgccc cagcggcaca tccacccagc gctacccagt
     6301 cctgggtttg ccaggcattc attgtccact gtataccctg cccgacaacg cttgcgccaa
     6361 acagagccag cccgcccatc accattttgc gccacaggct gcgtttcgga cgtaacgctg
     6421 cgtccattac cgcttcgact tgcccctctt cttcctgcgc ttcgtcgagc gtggccgggg
     6481 caaaattttg cgcctgattt tcgtcaaagg tctgctgcgc cctgaatttt ggattctgtt
     6541 cgacctccag cggaccgtcg aaatcaatac gtggttttaa cggttcggtc atcgcaattt
     6601 atctcctatt aaaaattcca gcgcggcatc aagacgaata tgcggcagcg gtttgtcgac
     6661 atccatcacc tgcgggcgaa acgcttcaaa ctggaaccct tgcttgtccc agaacgcctg
     6721 accgggcaaa cgcgcaggaa cttcaccggg gtaaacggtg agaggcgcac catcgctgag
     6781 ccggttaccg cgcaatgccg gaattttctc gccgttgacg tcaataatgc cgctggtggt
     6841 cgcctgcacc gaggccagcc ccaggcaatc catactaatc ctttcaaacg ccgcattttg
     6901 ccaggcatcc tgaatcagtt gttgcagtaa tgaaaccata ttggcgtgct gatcgaccgt
     6961 tacatggtcc gctttcatcg cggcaaacag cagtttgtcg ataaccggcg agaataagcg
     7021 gcgaaacagc gtgcgctgcc cgtagtggaa actttgcatc agctgcgtca gtgccagacg
     7081 catatcatta aatgcctgtg gcccactgtt gagaggttgc aggcaatcca ccagcacaat
     7141 ctggcggtca aagcgcagaa aatgattctt atagaacccc ttcaccactt tctcgcagta
     7201 ataattaaac cgctcgcgca gcattccggc attggtgtgc ttatcggcct gcgccagttt
     7261 ggactcgtcc caggcatcga catccggcca cgggaagaat tgcagcgcgg gcgcacctgc
     7321 catatctccc ggcaagacaa agcgcccagg ctgaataaag tgcagcccct gctgtttaca
     7381 gtggtggaga taatcggtcc acacggcggc aatgtctgcc agccggtttt cgtcggcagg
     7441 cgctagcggg tcaagtcctt cgcacatcat tcgccatttc gccgaccatt cgccgcgctg
     7501 accattgagt aagcccgtca tctggcgcga ccagcttaag taatcctgcg ccagcatcgg
     7561 caggtcgagc aaccattcgc cgggataatc cacaatttcc agatacagcg tggaggtatc
     7621 cttaaagtgg cgcagcagcg aatcgttcga tttaaaacgt aacgccaggc gaatttcact
     7681 gacgccgcgc gtaggcgtcg gccaggcggg aggatcacca tataactgcg ccagtccttc
     7741 atcataggtg aagcgcggaa tgccaaagtc acgctgggga atgcgtttta cgcccagcaa
     7801 gcgctcttca cgcaccgcgc ttaacagcgg caaacgtgct ccggcatgaa tattgagcaa
     7861 ctgattaacc atcgcggtga taaacgctgt tttgccgctg cggctaagtc cggttacagc
     7921 gaggcgcaga tgtctgtcga caccccgatt caccagcgca ttgagttcat ttttaagtcg
     7981 cttcattgct gtcctgtgct gctctgaaga ccagaaaaaa aatcattcgt aaggatatga
     8041 atgtgggggc caggccattg attatcaact gcccgtgttg tcatcgacgg gcgaactcca
     8101 taagatgtgc agcattcacg ttcggacgga gttcctgacc tatgtcccct actatttatg
     8161 atattgccag ggttgcaggc gtatcaaaat ccaccgtatc acgcgtgctg aataagcaaa
     8221 ctaatatctc cccggaagcg cgcgaaaaag tgttacgggc cattgaagaa ttacagtatc
     8281 agccaaacaa gctggcccgc gcgctgacct cttcgggttt tgatgccatt atggtgattt
     8341 ctacccgttc gaccaaaact acggcgggta atccgttttt ctctgaagtt ttgcatgcca
     8401 tcaccgccaa agcggaagaa gaaggtttcg acgtgatatt acaaacgtcg cacaatccgg
     8461 cagaagatct gcaaaaatgc gaaagcaaaa ttaaacagaa aatgattaaa ggcattatta
     8521 tgctcagttc gccagcggat aagtcatttt ttgcccaact cgataaatac gatattccgg
     8581 tggtggtaat tggcaaagtt gaaggtcaat atgcccatgt ttattctgtc gataccgata
     8641 attttggtga cagcattgcg ttgaccgatg cattaattga aagtgggcat caaaatattg
     8701 cctgcctgca tgctccgctt gatgtccatg tttcggttga tcgcgttaat ggctataagc
     8761 aaagtctgtc cgcgcataat attgcagtgc gtgataaatg gattgttgac ggcggttata
     8821 cccatgaaac agcactgcaa gcagcacggc aattattaag tcagtcaccg ttgcctgagg
     8881 cagtgtttgc cactgacagc ctgaaattaa tgagcattta tcgtgcggcg gcagagaaaa
     8941 atattgctat tccgcagcag ttagcggtgg tgggttatag taatgaaacg ctgtcgttta
     9001 ttttaacgcc tgcaccaggc ggcatcgatg ttccgacgca ggagttaggt caacaaagct
     9061 gcgagttatt attccgctta atttcgggaa aaccgtcacc acagaatatt accgttgcca
     9121 cgcatatgac gctgaaataa ataccagcta ccttgcctga atgccggatg cgatgctggc
     9181 gcatcttatc cggcctacat ttatagatcg gataaagtac tcgcgccgca tccggcaatt
     9241 tagcccatta tcagaacgag taatttacgc cgacacctgc ataatggaat ttatcgctgt
     9301 cgccttcgtc gtgatcctgc cactcaaacg cgtattccag cgaaacggaa agtccgttct
     9361 ggaaatcata accgtaaaat aaacccacac ggttaaaatc atggccttca cgttcaatat
     9421 catcctgcca gtcccagtta ctctagcgat ccagcccaat gcgcgtatac ggcgtcaccg
     9481 agtggttgcc gagcgtcagc ggcaaatagg cgcgaatttc ttgtgtggaa aactcaccgt
     9541 tattgcggct gtcgtccata ttgaagccgc gctcgagata atagttcact cgcaaagcaa
     9601 ccgtttcgtt gaaggtatat tgcagacctg tttccgtttc gacacgggta tcagcgtaac
     9661 cggtcgtgtt caaatcgttg gcaaatttat acatcgacaa ccaaccgttg aaacgtaaat
     9721 catcagtcag tttcacatcc cagtctggcg cgattttcca gcgctgcata ttcgccgtgt
     9781 ctttgcccgg ttcatcaacg tagtgataac cataattacg gaaaccgccg gtcaggccga
     9841 aactgaaatc atcgctttcg aggaactgat aatgcacctc cagctccggg cgatcaaacc
     9901 tcgttccacg tttacccgcg ctataatcta ccggcccttc ctgataatag gccagagcaa
     9961 ttctccacgg cccgttggcg gcattaaaat agactgaagg ctccgccagc ccatccatat
    10021 cttcgccata accctcgacg ttttctattt cgtacatcgc accgatatta aagtgccagt
    10081 cgctcttttc ctcggcctgt gcgcaggcca ttcccgcaca catcaccagt gcggtacagg
    10141 gtaataactt tttttgttcc ttgtattatc ctgggggaat gccttccccc tgtatttagc
    10201 gaattgctat ttccgtttct gcatcaaaga aatgacattt cgtcatatca aaatgaatag
    10261 tgatattttc tcctgcatga taatcattta acgcaccagc acggaccact aactcgtgcc
    10321 cccaaccgtg gtgtagagca taaattccgc accggttaat tctgccacgc taattttggc
    10381 ggaaatgtta ttttcctctt gcgcgtccgg atgaatatct tccgggcgta ttcccatcac
    10441 gatgggctta tgcaaacttt cctgtgtttt cagaaccgct aatttctctt cgagaatggt
    10501 taatttaagc gtttccgtaa cgaatttatc gccatcgatc gtgccgcgaa taaaattcat
    10561 cgctggtgat ccaataaatc cggcaacaaa catattcgcg ggttggttat aaacggtttt
    10621 cggcgcacct acttgctgaa caatcccgtc tttcataatc acaatccgcg ccgccatggt
    10681 catcgcttcg gtctgatcgt gggtcacgta gatcatggtg gtgttcagtt tctgatgcag
    10741 cttgctgatc tctgcacgca tttgtacgcg cagcttggca tcaaggttag agagcggttc
    10801 atccattaaa aacacgcccg cttcgcgcac aatcgcccgc ccgagcgcca cacgctggcg
    10861 ttgcccgccg gaaagcgcgc ccggcttgcg tttcaggtac tcacgcaggc cgagaatttg
    10921 cgccgcccag ttcacccgct catcaatcac ctctttggcg attttttgca tcttcaggcc
    10981 aaacgccatg ttgtcgtaaa ccgtcatatg cggatacaac gcgtagttct ggaacaccat
    11041 cgcgatattg cgtgctttgg ctggaacgtc attcattcgt ttgccgtcga tcaacagatc
    11101 gccgccgctg atctcctcaa gcccggcaat catgcgcagg gtggtcgatt taccgcagcc
    11161 cgacgggccg acaaatacga tgaactcttt atcggcaatt tccaggttga agtccttcac
    11221 cacatgcacc tggttatcgt agattttttg aatatgttgt aacgaaagct gagccatgtt
    11281 gattcctttg ctatacgttt tgccagaagg ccgataaccg cgaccaggtc agggagtccg
    11341 ttgagggcaa cagtaattgc gccccggtta agcccgcacc gatccccacc gagcgcatac
    11401 cgctggcgtt aatggcgtca atgcccgcct gcgcatcttc aatgccgata catgcctgcg
    11461 gcggcacgcc cagcccggca caggcagcga gaaagatttc cggatccggt ttcgagtttt
    11521 taagttggga agcatccgcg cagaaggtga aaaactcgcg cagctccagc gccgctaaaa
    11581 tcgtcggcgc attcagggag acggaagcca gccctaccgg aatctgctgt gcacggagct
    11641 ctgccagcaa attgcgaata ccgggcagca cagcattaac cgtcagctcg cgcagtgagt
    11701 ggacgtaaag cagatttttg cgatacgcca gttgcgcccg ctcctgcggg ttaaagtcgc
    11761 cctctttgcc cccgtgttgc agaatgcgcc gcagagactc atcgcggctg atccctttta
    11821 gagattcgtt aaactgcgca tcaatactga tgccaatttc agcggcaatc tgctgccacg
    11881 cctggaaatg cagatgcgcg gtatcggtga ttacaccatc cagatcgaaa attacccctt
    11941 gcagtttcat tcatcctcct gatgcgtggt agcggtccca ttgaagggca aaataaaatc
    12001 acccaaacag aaaacagatt cttcagccac ggatataagc tgaccgttca aacgcagtga
    12061 aacgggcgca gaagttcgaa tcgcaatacg ctgcgcgtcg agagtgacct gtaattcgca
    12121 gccctgccag aacagaggga aagacaactg ttgccactgc tcaagtaacg ccggatttag
    12181 atgcaattca ccgtcacgca cgctcacccc ggcaaaaccc tgaatcgccc ccagccagat
    12241 agcgccagtt gcggcagcat ggataccatc atcacaacta tgcggatcag caccaagatc
    12301 gatttcagtc ccctcgcgcc agaactgata actttgggtc agcaggccac agcgtgcagc
    12361 aacaatgccg tggattgctt tacttaatga cgagtcgtga atagtgcgcg gttcataaaa
    12421 ttgcagattg gcaagacacg atgccgatga gaactgctct ggcagcatgt aattgagcat
    12481 caccacatca gcttgtttga ggatctgcat ctcgttcact tctgcgcgtg aataatccag
    12541 cagaatggtt tgcttccccg ccgccgcttt gtatttcgcc agattaatcg ccggcttagt
    12601 cataaacgaa tcatcctgcg gcaaaacacc gtcgggctga atttctggca gccgcagttc
    12661 tttaaggaac atttcggcac gatgaataaa cgcgtcgtca ctacagccaa actggcgggc
    12721 aatactcagc gcctgctgga cgttgtaata caccatatag ctggtgaagg cgttattatt
    12781 gacatgttcg gtatattcgt ctggcccaat aacatcatga atttccagac ggtcgttaac
    12841 cctcaccgcg cggctaatcc agaactttgc agtttccaga agtagcgcca tgccttcatg
    12901 cgcaatgaaa ctttcatccc ccgtggtctg ccagtattga ataaccgccc aggcgatatc
    12961 ggccaccaga tgatgttccg cctgcgccga ggccactttt tgccgcagcc cggtgcgaat
    13021 gttaatggcg gcaaattccg gcgtctcttc ttcgccgctg cgcgcgcttt cccacggaaa
    13081 tagcgcgccc tgccagccgt tgcgtcgcgc tttctcctgc gcgcctggca agttgtgcca
    13141 gcgataacgc agtaaacttc gggcaaccgt cggatcgcta aacagatgga acggcagcaa
    13201 aaatacttct gtatcccaga aaacgtggcc tttgtagcct tcgccggtta agccttttgc
    13261 cgcaatactg ctgcgctcgc cgtgagccgg cgtcatgatg cgcagatgat aaagcgcata
    13321 atctaacgct tgctgatcgt gcgcatcgcc gccatttacc gtgatacgac gtttctgcca
    13381 ccattgacgc cagttttctg ttgatgctgc aagatgttgg tcgtaactct gttgcgcgca
    13441 catttcaagc tggcgaagcg acacgctgcc ccactcgtct aaagcagctt gcctgtcatc
    13501 ccgccagtcg atccacacca gtttttgcaa cgtcactgtc tcgcctgcat gaagctgcgt
    13561 actggtatgt tgcagtaaac ggcgctcttt ggcggtatag cattgctgca catcaccgct
    13621 cacctggcag caacaactga tggccacatc actgcgccca tcctgggtgg tgtagatccc
    13681 ctgcatcaaa tgctgaccaa acacccgcac ctgggtttcg tcgagatgct ggcgaccgtg
    13741 gttggtttgc gtggcgtcaa tgcctgttga aatcagcact gacgcgtcgg cgtccagtgg
    13801 cataatagtg atttccagcg caatgagcgg cagttggtct gccgaaacaa agcgacggct
    13861 ggcgatggtg taacctgcgc cgttgctggt acgccagaca acgctacggc gtaattctcc
    13921 actggcaaag tcaagttcac gctgccaggc ttcgcgggat aacgagaaaa cctcaccatt
    13981 tatggcaatc tccatcccca agatatcagg cagattcacc agttcgttga tttcaccttt
    14041 tcccgcccga tgatacagcc ccgccagata catccctcgc gtctggcgcg tgtaatcttc
    14101 ttcatggctg gcgcgaagcc caagatagcc gttcccctgc gccatcagcg atgcatactt
    14161 gttcagggta tgctggctga aatggggttc tgataacgtt actggcctgg tcataattcc
    14221 acacgcgtcc ctgtttctgc ggattgatat aacgccgcca ccagttgctg gatgatgtac
    14281 ccctgctcgg cgtcggctat catcacgggc ttgccctgta cgtgattgat aaaggcttcc
    14341 atgctgcgca aatggcggtt gtcgtctgcc atttcccgtt gcatcagcgt cattaattca
    14401 ccgttgttat cggtgtagat atgtgctgga aacagcgtcg caccggcttt atcaccacaa
    14461 aagctgacgt tcataatcaa ctgttcgcgg atgttgagtg caaatgacgt ttccagccac
    14521 agaatgccgc cgttatgaaa ttcaatggtg ccaaacagcg aatcttcgac gctgtaagtt
    14581 gccggatccc actcgccaaa ttgaccacag ctcttttgcg tgccgatctt ttgaaaacta
    14641 tgcgcattca cgcttttcac cgccggaaag cccagcacat acatcgcagc atccagcata
    14701 tgaatgccga tgtcgatcag caggccacca ccctgcagtt ctttattggt aaagacaccc
    14761 cagccgggaa cgccgcagcg acgcagggcg cgggcggtgg taacgtaaat ctctcccaaa
    14821 acgccgttgg tcacctgttc acgcagctgt tgcgtatcga gcgcaaacga tggtgaaagt
    14881 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccac gcctgttctg
    14941 tcgtcatggc gggcggtttt tcgcacatca catggcagcc tgcttccagt gccatcaggg
    15001 tatgttcgta atgaaaacgg ttaggtgagc cgacgctaac cacatcaggt ttcaccgcca
    15061 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
    15121 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
    15181 agtacgaagc atgaacctta tccgccacct ggcccgcgcc tattatcgcg acccgcagcg
    15241 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
    15301 aatcacggta cgcctgggca ggatcttctg cccggatacg cccttcatac accacataac
    15361 cctgatagtt atcggcgcgc agctgttcaa acagcgcgtg gaaatcgagg gtgccactgc
    15421 ccggctggta acgatggtta tcggcaatat gcacatgacc gagcaagtca cggttgtcgt
    15481 gcagcgcctg cgccaggtta tcttcttcga tgttcatgtg atagaaatcg ccgataatct
    15541 gcacatgttt aagattgttt tcgacgatgt aacgacgagc atcggcgagg gtgttgatca
    15601 tatgatcctg atagcggttt aacggttcga gatacaccac ggttccggta cgcgcggcga
    15661 cctgttccag tacgcgcagg gaatcactca ccagtttgcg gtcgccgtcc aggctacgcg
    15721 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga aaccccccac
    15781 ttctgccagc gcctcgagaa tgcgttcgat ctgctttaag ccattaagac gacgctcttc
    15841 gataaagtca ccaatccagc cgtcatagcc gccgcaggcg gtggtcactg gtaaaccggt
    15901 ttctttgatc gccgctttga cttcttcgat gttgttgacc agcagtttgc cgtcaatctc
    15961 aaaaccatcg aagcccatct ctttgatata acgaaatctc tcgcggatat tttccggaaa
    16021 gaatgcctga ttctgtgtgc cgattttcat tttttcattc ctttgaatta aaacgtaacg
    16081 cccattttga tgctctgttc cggatgctga tcgacatact gcatgtagct ttccgggctg
    16141 ttggcaaagg tcactaccgg gtcgattaaa tcttcgcaat tgagataacc gttcatcagc
    16201 agttcccagc aggtttcttc aatacgctta cggctccagc gcggataatc cgggttcggt
    16261 tcgctgcacg cgcgggagaa aacaattttg gcgttattga aatgcgcttc gcgtccgagg
    16321 ttaaaacctt cagcaaatgg tttggcaaac gcgacatagg agatggtgcc gccgtaggcc
    16381 aggccgcgca gcgccgattg cagcgcgtcg gcgtaaccgc tggtttcgat aatcacatca
    16441 gccccctgct tgccggtcag cgttttgatc tctttgccta cgtcagtgcc aatggggtta
    16501 aggcagaaat cagcaccgtg gcgacgggca atatcacagc gatgggcgat aggatcgacg
    16561 ccaattacca ccgatgcgcc agcgcgtttc gccagttgga tggcaatttg accgatcgcg
    16621 ccaagcccta ccaccacgac aaaatcccct acgcgtacgt tggcatcgcg cacgccgctc
    16681 atggcaaact gcgccgggtc gtaacagacg gcgtttttcc aggagctgcc ttgcggcatt
    16741 ttgcgcagct tgtagttatt cactgcgtta atgatgaccg tctcggagag cgggccgtag
    16801 ccgcatacgc tgtcgcccac cgcgtagtct gtaacgtcgc tgccgcactc gataatgtcg
    16861 ccaaccacca tgttgccaag ctggaatttg ccaaactcaa tgccgcgcgg cgcatctgcc
    16921 ggacgcgggg tgaacatctg ccactcgccg ttaaagtctt catcgataaa cgggctggcg
    16981 gcgcggaagt cgaccacttc cgttccgtgt ttcggtgcgc cgaaacgcac gcggatcttc
    17041 acttcattgg ctaaaatcgc ccgatcttca tactcaacca gcgcagcaac acgcggtgct
    17101 gtggctacta actttttcat gtaaaactcc ttgtaaagaa actggccgct ttagcccttc
    17161 acgccaccgg cggtcaaacc actcttaata aaacgttccg aaagcgcata cataatcacc
    17221 accggcaatg cggtcaccag cgaggccgcc atcatccgtc cccagatgta atcaggcgta
    17281 ctgaacagcg cgttcaggcc cactggtaag gtgaaattgc tggcgctgga gaggaaaatc
    17341 gacgcaaaca gatagtcgtt ccacgccacc ataaagcagt agacgaacac cgatatcagc
    17401 ccggacatcg ccaggggcac ggtgatacgg aagatgattt gcagacggtt gaggccatcc
    17461 atcatcgccg cttcttcgat ctcatcaggg atggtgtcga agtaactttt cagcataaac
    17521 acggcggtgg gcagggtttg cgtcaccatg gtaatgatca gcgccatctc ggtgtcgtaa
    17581 ataccaagcg cggtgatgat tttgaacaga ggcaccacca gcaaaatgcc agagaacata
    17641 tacaccgtgt aaaagctggc gttgatggtc atccggcctt taaagcgcag gcgggaaagc
    17701 gcgtaagctc cgagaatgcc gagaaatacc gccaccacgg aggaaaccac cgacaccacc
    17761 atgctgttac ggaagtagtc gacaaacggg aaaatcatcg ggttaaaaat gtcgacgtaa
    17821 tgctccagcg tccactgttg cggcagcagc gtaggatgca gcgatatcgc ctctttcgcg
    17881 cttttgaacg aggtcatcag catcacaaaa aacggaaaca gcgtgatgat gagaaacagc
    17941 gccagcccgc agtaaaaacc gatacgactg agcgtgcgtt tatttgttgc cattgaggtt
    18001 cacccgtttc ctggtcagca gaatgacagc gaaaataatg atgaagagca ccaccgagat
    18061 cgccgccgct ttccctaagt cattaaaggc aaaggcggtt ttgtagagat agacaccaag
    18121 aatatcgacc ttggttgtca gcagataaac atcagcgaac atgtagaaca tccagatggt
    18181 gcgcagggtc accaccgtcg ccaggaccgg cataattgcg ggcagcgtga cgatgcgaaa
    18241 ccgctgccag gcattagcgc catccatctc tgccgcttcg tacagcgatt tgtcgatggt
    18301 ctgcaaaatc gccagaaacg agataaaggc atacgggaag tagcgccaga tggcgaacag
    18361 caccaccagc acaaagctac tgcccggatt atcaaaccac agcggtgcct gctcatagag
    18421 atgcagaaga tcgacgccga ggtaattcac aatgccgtag ccgttgttga acatgtattt
    18481 ccaggcaaac accagcgaaa tggacggcgt tacgtaggag aggatcacca gcgaacgcgc
    18541 ggttttgcgc aggcggaact cgcggttgaa aaacatcgcc acggccagcc cgagcacggt
    18601 gctgcccgcc accaccagcg ccgtatacca gaccgtcatc cacagcgagt gccagaagcc
    18661 gggatcggag agaatgcgca catagttact caccccaaca aacgttgact cgatgttggg
    18721 attgagcggc agacgtaaaa aactgatttc gatattcgac accatcggcc acgccaccag
    18781 accgcccagc agtaataagc tgggcgcgag aagcagcagc gcaaacggca tatcggaacg
    18841 acctgaaaaa agcctgttca ttgcggattc ctgtcctggg tttagtgctg ttcgatcagt
    18901 tcatccagct ttttctggct cgcttgcagc gtgccaggga gatcggcttt acccacggtg
    18961 acgttatgca ccatggagct caccacacca gaacccgtca catcgcccat gcgggtaaag
    19021 tttttatccc ctactgcgcc aaagacctga atatttggca gttcaccgat tagctgattc
    19081 ggtagttcac ccagcgcctt aataacgtcg ttgtctttcc aggtggcggt agtgacaacc
    19141 gctttattca ccggcagcgc agcacctggc gacatcatca cccagtcggc aatgttgtcc
    19201 gcctgctcca taaaggtgac aaatttttct gctgcttcgg tcttttgtcc ggcggtaatg
    19261 gtcagcgagg tcaacatgcc gtagaccgca gagtttttct cggttggcac cacgaaaccg
    19321 acgtttttcg ggtcgccttc tttaatcaca gccggaagga tataggtgga gtaaatcgcc
    19381 atcggcgcgg tgccgttcat aaaggcgtct ttcacttcca tgatgtcgtt agaacccggc
    19441 atggtgttgg cagcaaggtc gcggtaatag gtcagcgcct gcatcatctc tggtgtatca
    19501 agggtgattt tgccttcggc gttaaagacg ttggcctagt tggataacgc aaactgggag
    19561 aaggattgtt ccgtcagcac gctttctgcc gtaggcagcg caataccgta ttttttattc
    19621 gccgggtcat tcagtttctg tgcaacgtcc agcagctgtt gccagttttt cggctcctcc
    19681 agccccgctt ttgccagcac atctttgcga taccagatgt cgccaatcca ggcgctgaca
    19741 ggaacaccgg tccatgcgct accatcttcg gtacgcacaa tacgcagtac gccatcgtaa
    19801 aacgcgcctt caccaacgtt gctgatgacc gtggcaaccg ctttgcgatc gataagctgc
    19861 tctttatcca taactttggc gtagtcatgg ctggtttcga tcacttccgg cagcgagccg
    19921 ctacgtgaaa gggtgatgac tttggtgtta tgagcgtctt cttccacggg cacttgttta
    19981 acggtgatac ctgggttttc tttttcaaaa cgggcaatca atttgctgat aactgcctga
    20041 cgctcctgct ccaccgaaga atgcataaat tcgatggata catgggtttt attttcttct
    20101 ttacagcctg aaatcagggc gcaggaaacc agtgctgata acagcacgat ttttgattta
    20161 atcatgagat gttccttttt atttaatcca cataacctgc caggggcgca gcgttatttc
    20221 attacccaat atatcaactt cactaatcag atcccgacca tgcagatcag taatatttac
    20281 atgctgaata tttttactga cattaaacaa tccagtcaga caattaccat cagcgttact
    20341 tctttgaata cgcattacgg atgaattaac cgtatcgatg gtaaaattat tatccggatg
    20401 aaactcgtta tggctgcgac gaagtgtaat taaacgcgac aactcatgat ataccgcatg
    20461 ccttaatgta gcttcatcgt tcagttctcg tgttatctct tcactgtgat attttttacg
    20521 gttaatcgca cggttatatc cgagtttttc gacacctgcg taatcattac gcgagccaag
    20581 aatactttga atatatatcg cgggtacacc ggggaaactt aacaaaatcg catgggcaag
    20641 gataaacctg gtgcaacgtt cttcatcgct actctcgcgg cggcttaacg cattcatata
    20701 ggtgacattc atctcatagg gactgcgcgt gccgtcggga ttatttttcc agtttact
//
LOCUS       DAEEGA010000070        20714 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEEGA010000070 DAEEGA010000000
VERSION     DAEEGA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20714
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.070"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(77..4537)
                     /gene="mukB"
                     /locus_tag="KLH46_002804"
     CDS             complement(77..4537)
                     /gene="mukB"
                     /locus_tag="KLH46_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBE0379422.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="KLH46_002805"
     CDS             complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="KLH46_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBE0379423.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="KLH46_002806"
     CDS             complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="KLH46_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBE0379424.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="KLH46_002807"
     CDS             complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="KLH46_002807"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBE0379425.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7462..8241
                     /gene="elyC"
                     /locus_tag="KLH46_002808"
     CDS             7462..8241
                     /gene="elyC"
                     /locus_tag="KLH46_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBE0379426.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8218..9111)
                     /locus_tag="KLH46_002809"
     CDS             complement(8218..9111)
                     /locus_tag="KLH46_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBE0379427.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="KLH46_002810"
     CDS             complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="KLH46_002810"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBE0379428.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="KLH46_002811"
     CDS             complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="KLH46_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBE0379429.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10242..11474)
                     /locus_tag="KLH46_002812"
     CDS             complement(10242..11474)
                     /locus_tag="KLH46_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBE0379430.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="KLH46_002813"
     CDS             complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="KLH46_002813"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBE0379431.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="KLH46_002814"
     CDS             complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="KLH46_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBE0379432.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14279..16538)
                     /locus_tag="KLH46_002815"
                     /pseudo
     CDS             complement(14279..16538)
                     /locus_tag="KLH46_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="KLH46_002816"
     CDS             complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="KLH46_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBE0379433.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="KLH46_002817"
     CDS             complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="KLH46_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBE0379434.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="KLH46_002818"
     CDS             complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="KLH46_002818"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBE0379435.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19833..20594)
                     /gene="ycaL"
                     /locus_tag="KLH46_002819"
     CDS             complement(19833..20594)
                     /gene="ycaL"
                     /locus_tag="KLH46_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBE0379436.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASG"
BASE COUNT         4983 a         5726 c         5032 g         4973 t
ORIGIN      
        1 ccgttatgtg ccattttttt agtgcagaaa cggagttttc ggaaaaagaa aaggcggcat
       61 tgctgccgcc ttaattttaa ctcgcctggg aaggcgcttc gtctcttcct agaagcgttt
      121 cagggagttg cggcgcaaat cctcgcaggc cgacgacgtg aacatgttcg gtattctgga
      181 agactttacg taccagttta taggtggtgc ctttttccgg actgatattt tccggcgctg
      241 cgatgatgag ctgcatttgc aaacgttcac acaattcaaa cagcgtggcg atagaacgag
      301 catccagtcg cgctgcttca tcgaggaaca gcaggcggca aggagagata tctttaccgc
      361 gcaggcggcg agattcatct tcccagcttt gtaccaccat caccagaatc gacataccgg
      421 taccaatcgc ctcaccggtc gacaatgcac cagactctgc gcgcaaccaa ccatcggaac
      481 cacggttaac ctcaacttcc atttccagat agttgcggta atccagcagt tcttcgccaa
      541 tggtctgcgg cgtgcgctgc cccatatcaa tctgcggatt aagacgctga tacaatttcg
      601 ccaacgcttc cgagaaggtc agacggttgc tgttaaacag atcctgatgc tgctcgtgct
      661 gttcagagag cacatccagc agcatggcgt gcgtttcacg cacgttcacg ttgagacgca
      721 cgctgttcac ctgaccaaac gatacgttct gcaacccctg gttgagcata cggatacggt
      781 tctgctcgcg ctgaatggtt ttgcgaatga tgttcgccac gctgcgggaa ctgatcgcca
      841 gtttctgttc acgggaggtt aattcttcgg tcagacggct aagttcaatc tccatctgtt
      901 cgatagcttc caccggatca tcggtacgaa taatatcctg acgaatacgt tcacgcagat
      961 gctgataaac cgccacgaag aactgaattt tacgctccgg acgtttcgga tcttccgaca
     1021 tgcgcagcac gtcgcgcaga tgttcgttat ccgccaccgc cagacgcagc gcacctaacg
     1081 ccttatccga catggaacgc aaatcatcag cggagagata agccagctca cgacggtgta
     1141 agcggcgctc aacgccgtta tctttcacca tgcgcatcac cgcacaccag cccgctttgg
     1201 cggtcactac ctgctcgcgc atctcaaagt aatcccgctc cagcttgcgc agtttgcggg
     1261 tcaggttgtc catctccgct tcgcagaagg taagcgcttt ttccagttga ttgcggcgtg
     1321 aacggttatt gctcagttgc gcatgcagct cgtcacggcg aatacgcgcc cgctcttctg
     1381 ccccgctatc agcacgcacg ccgatatcct gcaattcacg ttgcagatcg ttgagtagct
     1441 cttttttggt gtcgtaagaa cttttcagcg aagccagcac ctggttgtac tgactcaact
     1501 gcgctgcgtg accgcgcaac gcttcgcgag cgcgggtacg ctccgcttcc gcctgttcca
     1561 gacgttcacg cagtttttcg ttgagatcgc tgttaccgct aagcatttct gccgagtcag
     1621 aatagctaaa gtgcgcacga cgctgcacca cttccgtcag ggcaaacgct tgctgacggg
     1681 catcgcgctg catctgctga gagtacgcgt aatcttcttt taactgttcg aactgttccg
     1741 ggtcgctctg caataccgaa acgatcggtt ccagtttcgc cagttgattg ccaaactgct
     1801 gaacaaaacg cgcggcttcc tgggcttcat ccagacgttc gcggatttca tcgacgcgat
     1861 ccgccaggct gtcatcagcc aacaggttga gacgcggcag aatgcggttc agcgccgtaa
     1921 cgccttcttt cgcctgctca aactgaatac gctgctgctg gttatcattt tcatgattac
     1981 tcaacgcccg ctccagttcg actcgacggc tgttcagttg acggatttct gcttccgggt
     2041 cagactcaaa cgccaccgcc agatgactgc cgataaagcg gctgaacgcc tgatgcagac
     2101 gctgggtttt ctgtacatca aaggagagcg tggcgaagcg ttcggaaagc acttcacgct
     2161 cggcatggag gctttcaata cggctttcac gcgcagcacg accaaacagc ggcacttccg
     2221 ggaaacgtga ataacgccac tgtcgatcgg cgattttcac caccaccgct ttttccagct
     2281 catcaacgct gaacacgcta tcatcgaatg actgcggatc cccttcgatc aaatagagat
     2341 cttccgggca atcggtcaag ccttccaggt gttcggttac ctgtgacaga tctggcacca
     2401 cgatggcgtg gcgtgacggg ccatacagag ccgagaagta cggcgcatct tccaggctaa
     2461 cgtcgtcata aatttctgac agcagcacac caccaaaacg ctccgccagc gcgttcagac
     2521 gctgatcttc agagccgcca ggctggctta aacgttcgat ctcttcatcg acggcgtttt
     2581 tgcgcgcgcc tacttcatcg cgttcaacaa tcgcctcgcg ctcacgctcc agcaactgtt
     2641 gcagatattc agtgacgtcc tggctggagg agaactcttc gccgcactgt tcactcaact
     2701 ggttgagact gttttgcgct gccagccaaa ccggtgcacg ctgcatcaaa ctctgaatgc
     2761 gagactgcag ctgttcctgt tcctgccgca gtgccatccg ctcttcacgg gcgttagata
     2821 cgctatcgga aagagaggca atgcgtgctt ccagctcctg atgcagagct tccagttcat
     2881 caatatcaaa attcttgccc tgacgtttgc agaaatctgc cagcagacgc tcagcttctt
     2941 gctgctcacg cagacgctgt tccagttcgc ttaagcgcat ccgcaacggc tgaacctgct
     3001 ctgccaggtg acgctgatca accccttcgc gcaatagttc gcgagcgaca tcccacgcct
     3061 cgttacgcgc cagcgggccg ttgattgcca ccaccagctg ataagcctgc tcaaactggc
     3121 tgtgcgcggt ttgcgccatg ctcattttct gctcaagaga gagcattttt tcagtcgctt
     3181 ccagctcttt cgcctggaag gtttccagcc attcggcggc gctgtcggcg gttaagtccg
     3241 gcagatggca cagttctttg gcacgattaa gcgcagcaat cgcctggtta tactggatcg
     3301 cgcgcgtttg ctggacgtcc agcgcctgct ggtagtcagc aagctggctt ttcagctcgt
     3361 ccacttccag ttcggcagct tccgcgcgag cctcattctc ttcctggcgt tcgatggctt
     3421 ctgccaccac ttcattttgc tcttccagac ggatctgcag ctcatcgaga tccacttcgt
     3481 agcgttcgat cttctcctgc tgacgcaatg cggtttgcac caggttcagg tgatcgctgg
     3541 ccgcctgata atccgcttcc agatcgcctt cggcaccgtt gtgctctgcc agctcacgcg
     3601 ccatatcgac gtgtttgtac tgctcagccg ccagttgctg acgcgaagta tgtagctcgc
     3661 gacgaaactc cagggctttg tcgagatgga cacggcgctc gttggcgtga cgcatgtagt
     3721 ccgccgccac gtagttggta gcttcgctga tcagatgctt aaacaggtca cggtccgact
     3781 gggtgacacg aatcgcttcc agcgtcatgc ggttttcacg cagcgccgct tccatgtcct
     3841 ggaacgcttt acgcacgccg ctgttttccg gcaacaggta gtcgcgcaga gaacgggtaa
     3901 tggcactgga gatcccgcca tacagcgaag cttcgatcag acgatagaat ttgctacggt
     3961 cagatgcgga gcgcagacga cgcgcgatga tgcccagatc gaacatcagc gagtggtaat
     4021 cggtaatgga gttgaactgt ttaaactgca cgccttccat cgcctcgagc ttgtctttca
     4081 gctcgttaag cggcagcacg cgcgcctggc gttcgttcag ggtttcggtc accagctgtg
     4141 tcggctgcac cgacatcggc agtccctgaa tggcaaatgg tttgatatcc actttacgat
     4201 cgcgtccggc aacctgttgc agacgcacac cgaccaccac gcgctggtgg cgcgagttaa
     4261 tggtgtcgag catcgaataa cagacacccg ctttcagctt accgtgcaga cctttatcgc
     4321 gcgaaccgct ggtggccccg gcttccgtag tgttacggaa atgcagcagg gtcaggtcgg
     4381 ggatcagcgc cgtaacgaac gccgccatcg tggtggattt acccgccccg ttaccgccgg
     4441 aaagcgtcgt gaccagctcg tcaaggtcaa aagttcgggc aaaaaagccg ttccagttaa
     4501 tcagcgtcag tgagcgaaat ttaccgcgtt caatcattat tcttcctctc cgctatctgg
     4561 ctgactctct tcggtttcat cgttgagttg cagatgattt tcaatcggca ttgcttcgcc
     4621 atcacgaatc aggcgacgct gtgcttcacg gggatcgtcg ccagcacgca catcggctcc
     4681 gaagcggaac accgattcgg taatgcggaa cttgctgctg tcgtggccca taaaccacac
     4741 catgcctaaa cgacgcagac ggttgagcga agaacgtact ttctcctgca acttctgacg
     4801 gtcaacgtct gaaccggttg aacggttgtt caccagtttc agcagtttcg cttcatcggc
     4861 cagggtgagc agttcgtcgt acagttcctg ctgggtgaaa atcccctcat tcgccagccg
     4921 ttccgggctg agatagagat aacagaggat tttcccgacc atcatatcca gttccgacaa
     4981 gacggaacgc gggataagag tggtggaacg tgggcgtaaa tagaagaacc cttccggtgc
     5041 gcgaataagc tcaacgttat aacgcgcgta aaactcttcc agatattcct gaaaatccat
     5101 caggaatgca tgattatcca gttcgtcgag gccaatatgg cggcctgaac gtaaggcgct
     5161 gtccagcgcc ggaaataacg gattcgccag cgcctgcgcc agcttaaccg gcatcacttg
     5221 ttcaatattt gtcgatgaca tgcgcctgta ccttggctcc gtaatcatta atcggctgcc
     5281 atttcgctgg cagtccggtg aaatctgctt gcgctacgcc aagacgtacc gcctgatcaa
     5341 taacaatacg cgcaacgtca aagtgacgtg cacgcggata ctgtgacaga tattcgcgta
     5401 ccaccagacc aagatccagc ggcacttgtc tggttttgta cacggcaagt tgttcttcga
     5461 tgatcgccgc cagctgttcg cggatctcgt taaactcttc gtattccaga tcctcaggaa
     5521 gttccccagt cacttcttca tcgcgcagtg ccatctcttc gtcacgcata tccagcagac
     5581 gatcggcatt ggcataagtt agcgcccacg gctcatcaaa ataggtttgt accgactgac
     5641 gtaaccgctg agcaaagacg cggtttttat ccatatcgat cgcggtacga ataaatttgt
     5701 gtacgtggcg gtcgtagcca atccacaagt cgatggattg ctggccccaa ctgataatac
     5761 gatcgagttt gctctgcaga tcgaacacca gacgatcgac gaaatgcaga tcgtcatggg
     5821 tcatcgtcgc atcctgaatg cgcaacagat tagcctgcaa tttgtcgcct gccgcttcca
     5881 gcgtatcctg caattcacgc agcgttccgg aagtttccga aagcaacaat tcacagctgg
     5941 aaatcgccgc ccgccagtct ttgttcagca actgggcgat atcgtccttc acctgctgct
     6001 gctgttcgtc catcagacgt tgcgtcaggt cgatactgtc gaaaatttct gctaccgaat
     6061 atttcagtgg cgcatagaca ttacggtgcc agtgaaattc atcaccgccc tcttcggcgg
     6121 catctgctgc gcgtttgagc tcacccgcca caatcgacaa ctgcatagaa agacgcagcg
     6181 tagaaaactc gcgctgacgg atgtagtagt cagtaatgcc gatgccgagc ggcgtcagac
     6241 ggtaaattgc gttcccttcc gcctgctcgc tggtaaagcg gttcagcaga cgttgacgca
     6301 ccatgtcgtt gatcgcgtta ttggcgcgca cgccgatggt ttcgctggtt tgctcaaacg
     6361 catcactcac atggcggaat gcatccacca gctcgccttc actcatctca ccatccagac
     6421 gctcgccgtt cagcgtggca accgccagca gaaaagagag tcggtctacc ggcagcgaga
     6481 tggagaagtc attttttctg gcccaggcaa ccagttcggg gactgtctgg gaaaattcac
     6541 tcatacttta tccttgctct gcggtttgcg cgcggtgaca tgaatataac gccctagggt
     6601 aatatacggt tcctgacggc aataacgcgt ttctaattca agtaatgctt catagcagtc
     6661 gcgctgctgg tacttctcgc gcagataatc atgaaacacg cgaacgcctg tcttacccat
     6721 aatttgccaa ccagcttctt ccagccacag ataaacctgc gccgggtcgc gtggataatc
     6781 tggcgaaagc gtccgttttt tctttttcgg cattcccgcc tgcacgtaat caaaattccc
     6841 ggcgaccatg ttatgcatca acaaaccatg cgcattgtag aacattaacg acaacactcc
     6901 gcctggacgc aacactgacc acagggtctg caatacactg cggggatcgg ccacccactc
     6961 gagcaccgca tggaacaata tcagatcaac gggcgtttcc aaatgcgaag caacatcctg
     7021 agcggcgcaa tgtataaatt gcatgttgtc gctcacacct tttgcttctg ccgcctgttt
     7081 tgcgcggtcg atcatctgcg cagaaagatc gcataaaata acctgatgcc cgcgctcggc
     7141 cattttgatt gcggtctgcc cttctccgcc gccagcatcc agcacacgca gtttttgcgg
     7201 gcccatttcc gccagcacgc gatcgagatc ctgccacaga atagcctgtc gaagctgccc
     7261 tttggtggtg ccgtaaatgt tacgggaaaa cttttccgca atatcatcaa aattgcgatc
     7321 ctgcattgct ttctccaccg gttggctaag acgttttctg ataaagacgc tattttgtca
     7381 cagccacggc gagaatgaac ccgtctggtg taatatcgct agcgatccct tgctatatgg
     7441 ttaaaaaagg aaccagaaag gatgcttttt acactgaaaa aagtgattgg caatatgttg
     7501 ctgccacttc cgctgatgtt gttaattatt ggcgctggcc tggcgctgct gtggtttagc
     7561 cgttttcaaa aaaccgggaa gatttttatt agcattgggt ggctggcact cttgttattg
     7621 agtttgcaac cggtagccga ccgcctgctg cgtcccatcg aaagcaccta tccgacatgg
     7681 aataattcgc aaaaagtgga ctatatcgtg gtgctcggag gtggttatac ctggaacccg
     7741 cagtgggcac cgagctctaa tttaatcaat aacagtctgc cacgcctgaa tgagggtatc
     7801 cgtttatggc gcgaaaatcc gggttcaaaa ctgatcttca cgggaggcgt agcaaaaacc
     7861 aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg cgtaccgcgc
     7921 gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc ggcagtgaaa
     7981 caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct gccgcgggcg
     8041 atgatctttt ttcagcagga agggttaaat ccgctaccag ccccggcgaa tcagttggcc
     8101 atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtctg gctgatgcat
     8161 agcgatcgtg ttggctatga aacgcttggc cgcatctggc agtggctgaa aggtccgtca
     8221 ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac taaatcgtcc
     8281 ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc gccagatgaa
     8341 cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg ccagagaatt
     8401 atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc ccggcccgac
     8461 catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac agaagttacc
     8521 gtgaatcaac acacaattgt cgttaaagcc ttcaaataat gccgggagac attcgcgagt
     8581 gcgaaacagg atccgcttat cctgcatcgt cagaccggtg ttattgaact gattgagcgt
     8641 ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt cctgagtatt
     8701 gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta aggcttcaac
     8761 gatttggtct ttgagctgtt cccagcgttc tggtgttcgg gctggtgcct ccaccgaaac
     8821 accacgcatc cgctccagta gcaggacatc agggccgggg tgttcttcat gcgtcatcac
     8881 accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg ttttccatgc
     8941 cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca ttgggtttcc
     9001 ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc actcaatacg
     9061 actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca ttcagattac
     9121 cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca cctgaatgag
     9181 atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc ctcccaaaat
     9241 tggctgaagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct caagatcttc
     9301 aggggtatcc acacctgtgc caggaacttc ctgagcaaca gcaacatgga ttttttcgcc
     9361 gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac ttgcctgcca
     9421 gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat gacgcaggaa
     9481 gttatcgcca acggtttcaa ggccttctgc aaaacgatca cgatcccaag gaatggtggc
     9541 acgtgagaag tacagtgcat acccttcagc gtcgagaacc actttcaccg cattcgggtt
     9601 aaacgcttct tccgcattgt ggattggcac cgccagagtc gccataccca cctgacgctg
     9661 agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt catcaccctg
     9721 cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt ccgccagacg
     9781 ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag cggcttcaac
     9841 ggcgcgggca acatcatcat gatcggttgc cacgatgatg cgctcggcac ctgattcacg
     9901 cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca atggtttacc
     9961 gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca tgatttactc
    10021 tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc atcgcggagc
    10081 gggaaagcca ggttatccag tttgcaaata agctcttgtt tttcctggtt ataccaaagt
    10141 tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac gatgatccat agttcctcca
    10201 gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac ggggtctatt
    10261 tcccagccag tgcggcaatc agtaaagaga ccttgcgggc agcgtcctaa tgtcacccgc
    10321 gttgcctgct gccagctcgc gaaatcagta atcgcctgac gtaacccttt ttgcagcatt
    10381 gtcgttggtt taatgccttc ctgtaaccac agagagataa cttcaaggat gcctgtctgg
    10441 cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa tggcagaaca
    10501 aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta gctaaaatca
    10561 aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga aagtaccgcg
    10621 ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag caaatcagca
    10681 tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg ctgttcagcc
    10741 ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc gatagtaatc tgccagccac
    10801 tgttcgcgga atattcccag gctacgcgca ctgttatcca gcatgatgat ttctgcttct
    10861 gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacggtg ggttaaatca
    10921 taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt aaataaacct
    10981 tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt acgaggatgc
    11041 tcaaaatcgg ctgaacgtac cggcccttta tcatgaatat gctgaattaa ctgtgcaatt
    11101 tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt ttcaggtgcc
    11161 agcatgcggt ggcgaataag acgaaagtcg ctacgcggca taaagcaggc ttcatgcgcc
    11221 cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc aggataattt
    11281 cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat ggtatcgatt
    11341 tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgacgc tcgacggcgg
    11401 ggtttgttta acaggccttg tgcggcaagg tgaagattac gcgcatcagc aagggagagg
    11461 tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa ctagttgcca
    11521 gaagccagcg aggttagttg cgtaagcagt ttcgctggtt catcacctga aagctgtgcg
    11581 tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac cgcatctttt
    11641 tcagtcatta ccagcgtttg cccggcgctt accaacgcac tgacatccgc atggttcaaa
    11701 gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat cttcagcgtg
    11761 gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca ctacatgttc aagctgagca
    11821 acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag atgcatgggg
    11881 atttcaccgc tgcgagggac accgccgttg acgattaccg catcaaccga ctttaagcgc
    11941 cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa gcgacgcaca
    12001 ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa accgtcgtcg
    12061 gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc atcagaacga
    12121 acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg ttcatcaccc
    12181 gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc cttaccacca
    12241 tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc caccagccag
    12301 acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac gacaaccggt
    12361 acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat cgcgccactc
    12421 accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga ttcaccagac
    12481 cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa acgccgcggt
    12541 gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg acgaccacga
    12601 tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag gtacggtttt
    12661 tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc agagccgagg
    12721 tagcttcatc cagaatcaga atcgggctat cacgcagcaa ggctcgagcg atagcaatac
    12781 gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta tcgagaccgt
    12841 tatccatctt attgatgaag tccatggcgt aggccatccg cgccgcttct tcaatttgct
    12901 cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta tcgttaaaca
    12961 gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg gtatactcgc
    13021 gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa cgcgtgatca
    13081 ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc gtcttccctg
    13141 ccggaatttt caggttgatg ttacgcaatg cgggtacgtc acgtcccgga taagtaaagg
    13201 tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta ccttcgtctt
    13261 tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata ccgcgctgga
    13321 actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc attgaagaga
    13381 aaacaacggt aatcgtaccg gcagtcaggt tatccataac acttgggaag ctcgccgcat
    13441 acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca gagatggaag
    13501 aggctgaaac cattttcatc ccctgaagac gcattcggtt actgacttta tcgaagcgtt
    13561 tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc agcatttgtt
    13621 ctgcactggt ggtcacttgc cccatggtgt tctgcatgtt tttactgatg ttgcgaaaac
    13681 gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc aaaatgatcg
    13741 acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac gcaccttcac
    13801 gcacaacagt aatcagtgcg ccggaagaag aagaagcaac ctgttcggaa tcgtaggtga
    13861 tacgtgacaa caacgtaccc gttgactgtt tgtcaaagaa tgaaactggc attcccatca
    13921 tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc caggagatac
    13981 agtagctgga gacatagctg gtgataccac gtaaaatcat cagtccgatc accaccagcg
    14041 gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga agtggcttaa
    14101 ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg cccgccacga
    14161 tcagacccgc tttgaaaggc gcaatggttg gccacagtcg gcggaatgtc tgccacgtag
    14221 agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc cgcatattct
    14281 acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa atttgctctc
    14341 tgaggctgct aatccgccag ccttgtgctg aaaaattgac cgtcacttga ccctgatgtg
    14401 gagtatcaag ccattgatat cctttctgtt gatagcgatg cttaactttg ttagagggca
    14461 atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc actcgctgaa
    14521 ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc aatgttgcct
    14581 gcacttgctg ccagtaacgg cttagcatct tttgttcagc tggggcttca atatcaccgg
    14641 ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg ttatttccct
    14701 tatcgttgct accttgtaaa ggccagtgcg cgctgaaacg caatccttgc cactgccacg
    14761 cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg atccataaca
    14821 tcggccatat atgcaatatt gaatccagcc ctccccggtg atccagatgt tcatggctca
    14881 gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc agttgttgcc
    14941 cactatcccc ttcaggccag tcctgtgtca tagagaatcg ctttgccgtt tctggctatc
    15001 accattgcca gcccttgccc gacatcaagc atgtacagct gccactcgtc aggtcgaggt
    15061 ttttgccaca gcggccagca catcagcaag cctccagcca cacacattgc tggcaacgtt
    15121 cgccaggcgt ttaatcgcca taccaccagt aagaaccatg gggaaaatga tagccattgc
    15181 caacattcaa caatgttgat ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa
    15241 gccaaagacc ggtcggcaag aaaccataac ccctgttcta ggattaacgg cccgctaaaa
    15301 tgcacaacca tcgcggcgag gatcaacgga accgtgataa atgtcaccaa gggaattgca
    15361 aatagatttg caataaacga ggtcagacta atgccatgaa atatgacgat ttgcatgggc
    15421 ataagcagga gtgtgattcc cagttgcaga tggatgaggg aaacaactgc acgcaatacc
    15481 ggcggcagtt gccactcagg acagggaaac cactgatacc aaaaaatcag tgccgcgacc
    15541 gcagcggcag agagccataa actttgcgag agaatggcaa caggatccat cagcaaaatt
    15601 gccgccagac aacatatcca tacatcccag ccactccact gtcgcccact taacttaagc
    15661 attccccacg tagcaagcgc caccacggta cgcaatgcag gaggttgcat tcccgtcaac
    15721 caggcataaa aagcagcaca gcagattccg ccaattaatg gtatttgcca gtggatccag
    15781 cgcccaggca gaaaaatttg tccactgcga atgagtccgg cagccagcaa cgccgcaaaa
    15841 gcgatgtgca atcccgatat cgccattaaa tgcgccgttc cagtatcgcg catgatattt
    15901 ttgatttctt tggggacgga taaccgttcc cccataccta aaccaagaat aaccgcattc
    15961 cacggatagg gttgcagcgt tgtttgtagt gacgccagat actgtgcacg caggctacaa
    16021 ttcggttcaa tgacacttgc ctgcagaaaa cggccggtga gcggctgatg ctgggcaatg
    16081 gcataacgct ggctatcaaa gccgccatca ttaagttggc catgaactgc acgaactttg
    16141 agtttcattg accattgttg tccagcacaa accgcttgcg gaagttattc gccatacagc
    16201 acaaggcctg gcgcagggaa tatacgtcga ccttgtagat aagtaatttg accgtaatga
    16261 gtggtcatgc cgtcagttgc agtgatctca acaattgcat cctgcgtcgc gccagttaag
    16321 gtttctcctg cccacaaaat ttgctttgct gataatatgc cccaaacgaa aaacagcaac
    16381 gtcagcgcga tataacggac ggttttaaca gggataaatg ccagtacgca ggcgaagaga
    16441 gtcagaaacg caagggttaa tgtcctaggc aattggggca aaatcagcaa cggaaaaatt
    16501 ccgcaaatta tgcatacgcc gactgtcgtt attttcatgc acccctccct gtttatggaa
    16561 agtgtgcaac tttgttgagc ggttgtctgg tgggaatttt gcgttccccg aggcacattc
    16621 agaagaattt aacgaaaggg tgaaaactgc tcaaaactct gcgaggcagc ttccagttcc
    16681 tgattagaga attgaaaaaa gcacccgaca ggtgcttttc tcgcgttcaa gtttgagtaa
    16741 aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt accaggttta aagtgaggaa
    16801 cgtattttcc ttccagttct actttatcgc cagtcttcgg attacgtccg gtacgtggtg
    16861 cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc aatacgctcg ccctgcgcaa
    16921 gagtcgaggc catatgctcc agcatctctt ttactgcatc ttcaaccgtc ttggcgggaa
    16981 tgtgcgattg ctgggtggca agtctttcta tcaattctga cttggtcatg attcctccgg
    17041 ttccttaaag gcaaattaag cggctgctgt tgctgcattg attaagggcg gccgtagccg
    17101 cccttagtgc ttgattacag gacgaaacct gcaatctgtc aagtaaactc aacaaacttc
    17161 ggaataaaaa tcccgaagag tcagagaatt actcgccttt agctgctttg aaagcttcag
    17221 ccattgcgtt gttggagaag tttgcatctt cctgtttgtt aacagttgcg attgcatctt
    17281 tctcgtcagc ttcgtctttc gcacgaacag acaggctgat tgcgcggttt ttacgatcaa
    17341 cgccggtgaa tttagcttca acttcgtcgc caacgctcag aaccagggta gcgtcttcaa
    17401 cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc aacgccgtca gccagttcta
    17461 cggttgcgcc tttagcgtca actgcagtta ctttaccggt tacgatagcg cctttcttgt
    17521 tcagagcaac ccagttgttg aacggatctt ctgcgagctg tttaacgccc agggagatac
    17581 gttcacgttc tgcgtcaacc tgcagaacaa ctgcagcgat ttcgtcgcct tttttgtatt
    17641 cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc agacaggtga accaggccgt
    17701 cgatgccgcc gtccaggccg atgaagatac cgaagtcagt gatagacttg attttacctt
    17761 caacacggtc gcccttgttg tgggtttccg cgaactgctg ccacgggtta gctttgcact
    17821 gtttcagacc cagggagata cgacgacgtt cttcgtcgat atccagaacc ataacttcca
    17881 ctacatcgcc aacgttaaca actttggacg ggtggatgtt tttgttggtc caatccattt
    17941 cggaaacgtg taccagacct tcaacgcctt cttcgatttc aacgaagcag ccgtagtcag
    18001 tcaggttggt cacgcgacca gtcagtttgg taccttccgg ataacgttta gcgatagcta
    18061 cccacggatc ttcgcccagc tgtttcaggc ccagggatac acgggtacgt tcgcggtcga
    18121 acttcagcac tttaacagtg atttcgtcgc ccacgttgac gatttcgctc ggatgcttaa
    18181 cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc aacgccgccc agatcaacga
    18241 atgcaccgta gtcagtgagg ttcttaacga tacctttaac ttccatgcct tcctgcaggt
    18301 tttccagcag ctgatcgcgc tctgcgctgt tttcggattc gataacggca cgacgagaaa
    18361 caacaacgtt gttgcgcttc tgatccagct tgattacttt aaattcaagc tctttgcctt
    18421 ccaggtgcag agtgtcacgc accggacgaa cgtctaccag agaacctggc aggaacgcac
    18481 gaataccgtt cagctcaaca gtgaagccgc ccttaacttt gccgttgata acaccggtaa
    18541 cagtttcagc atcttcgtaa gctttttcca gcgtgatcca ggcttcgtga cgtttagctt
    18601 tctcacggga cagcagagtt tcaccgaagc cgtcttctac tgcgtccaga gcaacgtcaa
    18661 cttcgtcacc tacctggatt tccagctcgc cctgggcgtt tttgaactgc tcagccggga
    18721 tagcggactc agatttcaga ccagcgtcaa ccagtactac gtctttgtcg atagcaacaa
    18781 caacgccacg aacgatagaa cccgggcggg tttcgatttc ttttaaggac tcttcaaaga
    18841 gttgagcaaa agattcagtc atgtttaatc ttcaggttta tatttaacgt ccacctggct
    18901 ccatgccgga tggggttgtt taacataccc gctgtcattc cattgcaacg ggggtactgc
    18961 aaattcggtc gcttatgcga gagccaattt ctggcgcgcg tattgtagcg ctttttcaat
    19021 caattgctca atgcttaagg tggtggaatc caacactaaa gcatcggctg ccggaaccag
    19081 tggcgctacc gctcggttac gatcgcggtc gtcgcgttct ttgatctcgg ccaaaaggcg
    19141 ctcgaagtta acactaaagc ccttctcctg caactgtagc atgcggcgat gcgcacgttc
    19201 ttccgaggag gcgtcaagga aaattttcac tggtgcatca gggaatacca ccgttcccat
    19261 gtcgcggcca tcggcaatca gacctggtaa ttcgcgaaac gcgcgttggc gacgcaataa
    19321 tgcttcacga acgcgtggga atgccgcgac ttgtgaagcc gcattcgcca cttcctgagt
    19381 acgaatttcg ccgctgacat cttccccttc gaggatcact tccagattgc cattggtcga
    19441 cacaaaacgt acatccagat gggatgccag cggtaccagc gcatcttccg acgcaacatc
    19501 aacatggtga tgtaatgccg ccaatgccag tacgcgataa attgcacccg agtccagcag
    19561 atgccattgc aacgcttccg ccatagcctt acacaaggtg cctttccctg caccgcttgg
    19621 gccatcaatg gtaataaccg gggcaattgc cgtcatcttt atctccttaa aacaggcgta
    19681 ccgttaacat aaccgcgcgc attatacgcg ccgacgccac aaatcgttaa cgttgtgtgc
    19741 aaaaaccagt tttgtctgta ttcagatggg gatttattgc gcaattatag cgggctggca
    19801 gaagaccagc ccgaaagatg acaatgattt acttagccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccaggctag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaagagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacgaacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat gtgc
//
LOCUS       DAEEGA010000071        20699 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEEGA010000071 DAEEGA010000000
VERSION     DAEEGA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20699
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.071"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            81..350
                     /locus_tag="KLH46_002820"
     CDS             81..350
                     /locus_tag="KLH46_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBE0379437.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="KLH46_002821"
     CDS             344..709
                     /locus_tag="KLH46_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBE0379438.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="KLH46_002822"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="KLH46_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBE0379439.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="KLH46_002823"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="KLH46_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBE0379440.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="KLH46_002824"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="KLH46_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBE0379441.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="KLH46_002825"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="KLH46_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBE0379442.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="KLH46_002826"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="KLH46_002826"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBE0379443.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="KLH46_002827"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="KLH46_002827"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBE0379444.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLNAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="KLH46_002828"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="KLH46_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="KLH46_002829"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="KLH46_002829"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBE0379445.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="KLH46_002830"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="KLH46_002830"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBE0379446.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14857..16455
                     /gene="rtcR"
                     /locus_tag="KLH46_002831"
     CDS             14857..16455
                     /gene="rtcR"
                     /locus_tag="KLH46_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HBE0379447.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16437..17195)
                     /locus_tag="KLH46_002832"
     CDS             complement(16437..17195)
                     /locus_tag="KLH46_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBE0379448.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17212..18042)
                     /gene="glpG"
                     /locus_tag="KLH46_002833"
     CDS             complement(17212..18042)
                     /gene="glpG"
                     /locus_tag="KLH46_002833"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBE0379449.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18087..18413)
                     /gene="glpE"
                     /locus_tag="KLH46_002834"
     CDS             complement(18087..18413)
                     /gene="glpE"
                     /locus_tag="KLH46_002834"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBE0379450.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18603..20108
                     /gene="glpD"
                     /locus_tag="KLH46_002835"
     CDS             18603..20108
                     /gene="glpD"
                     /locus_tag="KLH46_002835"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBE0379451.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20377..20607)
                     /locus_tag="KLH46_002836"
                     /pseudo
     CDS             complement(20377..20607)
                     /locus_tag="KLH46_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4889 a         5693 c         5219 g         4898 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtt cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cgcttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg caccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttttt aatttattgt
    14761 ttctaaaaag attatatctt tatgtccgta aacggagatt tcccgcaaag ccgatttacc
    14821 gataatgaaa tatcgccttt tataaggata tctaagatgc gtaaaacagt ggcttttggc
    14881 tttgtcggta ccgtgctgga ttatgccgga cgcggcagtc agcgctggtc aaaatggcgt
    14941 ccgtcactct gtatatacca gcaagaatcg ttggtcatcg atcgactgga attattgcac
    15001 gatgcccgct cgcgctcgct atttgaaacg cttaaacgcg atatcgccag cgtttcgcca
    15061 gaaacagaag tggtgggcgt tgagattgaa ctgcataacc cgtgggattt cgaagaggtt
    15121 tacgcctgcc tgcatgattt cgcccgtggt tacgagtttc agccagaaaa agaagactat
    15181 ttaattcaca tcaccaccgg cacccacgtc gcgcagattt gctggtttct gctggcggaa
    15241 gcgcgttatc tacccgcccg gttgatacaa tcttcaactc cgcgcaaaaa agaacagccg
    15301 cgcggcccag gtgaagtgac gattatcgat ctcgatttaa gccgttataa cgccatcgcc
    15361 agccgctttg ccgaggaacg gcagcaaacg ctcgatttcc tcaaatccgg tattgccacg
    15421 cgtaaccccc acttcaaccg catgattgag cagatcgaaa aagtggcgat taaatcccgc
    15481 gcgccgattc tgcttaacgg cccgaccggc gcaggtaagt catttctggc gcgacgcatc
    15541 ttcgagttaa aacaggcacg gcatcagttt agcggcgcgt ttgtggaagt gaactgcgcc
    15601 accctgcgcg gcgataccgc gatgtcgacg ctgtttggtc atgtgaaagg cgcgtttacc
    15661 ggggcgcggg aatctcgtga aggattatta cgcagcgcca acggcggaat gttgtttctt
    15721 gatgagattg gcgaactggg cgcagacgaa caggcaatgc tgctgaaagc tattgaagag
    15781 aaaacctttt acccgtttgg cagcgatcgc caggtgagca gtgattttca gcttatcgcc
    15841 ggaacggtgc gcgatttgcg ccagctggtt gccgaaggca aattccgcga agatctgtac
    15901 gcgcggatca atctctggac cttcaccctg ccgggtctac gccagcgcca ggaagatatt
    15961 gaaccgaacc tggattatga agtggagcgc cacgccacac tcactggtga cagcgtgcgt
    16021 tttaacaccg aagcgcggcg tgcctggctg gcctttgcaa catctcccca ggcaacatgg
    16081 cgcggtaact ttcgcgaact ttctgccagc gtcacgcgga tggccacctt tgccactagc
    16141 ggacgcatca ctctggacgt ggtggaagat gagataaacc gtctgcgcta taactggcag
    16201 gagagccgcc cctccgcgct tacggcgttg ctgggcgcag aggcagaaaa catcgatctc
    16261 ttcgaccgta tgcaactgga acacgttatc gctatctgcc gccaggcaaa gtcgctttct
    16321 gctgccggac gacagctttt tgatgtttcg cgccagggca aagccagcgt caatgacgcg
    16381 gatcggctac gcaaatacct ggcgcgtttt ggtctgacgt gggaagccgt gcaggatcag
    16441 cacagctcca gttgaatatg gtggtccgtc agcacctgca tcacgcttac tggcggcggg
    16501 gcgtcggtgt agacggcatc aaccatgctg atgctcccca tattgaccat cgcattacgg
    16561 ccaaattttg aatggtcaac caccagcata acgtggcgcg agttctcaat aatggcgcgc
    16621 ttggtacgaa cttcgtggta atcgaactcc agcagcgagc cgtcgctgtc gatgccgctg
    16681 atccccagaa tgccgaaatc aaggcggaac tgggagataa aatcgagcgt cgcttcgcca
    16741 atgatcccgc catcgcggct acgtaattcg ccaccggcga gaataatgcg gaaatcttct
    16801 tttaccatca acgtgttagc aacgttgaga ttgttggtga caatgcgcaa attgctgtga
    16861 ttgagcagcg cgtgcgccac cgcttccggc gtggtgccga tatcgataaa cagcgtcgag
    16921 ccattgggga tttgctccgc cactttgcgg gcgatgcgct ctttttcttc ggtctgggtg
    16981 gctttacgat cgtgccacgg cgtgttaacc gaactggaag gcagcgccgc gccgccatga
    17041 tggcgcagga tcaggttttg ctctgccagc tcgttgaggt cgcggcgaat agtctgcggg
    17101 ctgacggaga agtgctccac cagttcctcg gtactgacat aaccctgctg tttaaccagt
    17161 tcgataatac cgttgtgacg ttgtgtttgt ttcatttata aatccctgga attattttcg
    17221 ttttcgcgca ttgagcgaat caacaaaagc catcgctaaa cccacggcta atccggcgat
    17281 gtgtgctccg ttcgccatcg acatcccaaa caaatcaaac catccggcga caatccagat
    17341 cagcgcaaag ataattaacc cacgttgcag gtaaatgcca ctttgcggat cgcgttcgcc
    17401 acgtagccag acgtagccca tcagcgcata caccacgcca gaaagcccgc caaaccacgg
    17461 cccgctgaat ttttgctgca tatagccgct taacagggcg ctgataagcg taatgacaat
    17521 tagcttaccg ctaccgaggc gtttttccac cgcgccgccg agataccacc accaaagcag
    17581 gttaaagagg atatgcatca gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa
    17641 ctcaaatttc agtgttggat cgaatggcca ggccagccat aacatcactt cctgatcgcc
    17701 gagaatttgc atggcaataa acaccaccac gcaggcgatc atcatcaccc aggtcaccgg
    17761 acctgcgcgt tcacgcaagg cagcaaagaa aggataacgg cgataatgca ggccactgcc
    17821 ggtatggcct gccagccagc tggccgccag ataacgcgga tctgccgggt tttcgagaaa
    17881 acgcgccagc tccgcccgca cgcgctcggc ctgggactca tccgccagcc agacatcgct
    17941 ttggttatgt tgttgaatcg tgaggataac accctgcgtc gccatgtaat caacaaacgc
    18001 ctgcgccacg cgggggttag caaaagaggt aatcatcaac atcgttgctg tcgcttattc
    18061 cacacaaaag gggacagtat aaagcgttac gcgccgtacg ccacctctgc gggaaactgt
    18121 cgctgccagg cttcaaagcc gccgtcaatg ctatagacca catcgtagcc ctgttgcagc
    18181 agatactgcg ccgccccttt gctgctattg ccgtgataac acatcaccat caccggagtg
    18241 tcaaagtcgt tatcacgcat aaacgcgccc agcgtgtcgt tggttaaatg gaaagcctgc
    18301 accgcatgcc ccatagcgaa actctgtgga tcgcgaatat cgaccagcac cgcctctttt
    18361 tcctgcaact tctggtgcgc gtcggcaacg ttaatacatt cgaactgatc catgcgtctc
    18421 tctttcttta caaacaagtg ggcaaattta ccgcacagtc tacgtcgaag cggcagataa
    18481 acgccataat gttatacata tcactctaaa atgttttttc aatgttaccc aaagcgcgat
    18541 tctttgctaa tatgttcgat aacgaacatt tatgagcttt aacgaaagtg aatgagggca
    18601 gcatggaaac caaagatctg attgtgatag gtggcggcat caatggtgct ggtatcgcgg
    18661 cagacgccgc tggacgcggt ttatccgtgc tgatgctgga ggcgcaggat ctcgcttgcg
    18721 cgacctcttc cgccagttca aaactcattc acggtggcct gcgctacctt gagcactatg
    18781 aattccgcct ggtcagcgag gcgctggctg aacgtgaagt gctgctgaaa atggctccgc
    18841 atatcgcctt cccgatgcgt tttcgcctgc cacatcgtcc gcatctgcgc ccggcgtgga
    18901 tgattcgcat tggtctgttt atgtacgatc atctgggtaa acgcaccagc ctgccgggat
    18961 caactggttt gcgttttggc gcaaattcag tgttaaaacc ggaaattaag cgcggattcg
    19021 aatattctga ctgttgggta gacgacgccc gtctggtact cgccaacgcc cagatggtgg
    19081 tgcgtaaagg cggcgaagtg cttactcgga ctcgcgccac ctctgctcgc cgcgaaaacg
    19141 gcctgtggat tgtggaagcg gaagatatcg ataccggcaa aaaatatagc tggcaagcgc
    19201 gcggcctggt taacgccacc ggcccgtggg tgaaacagtt cttcgacgag gggatgcatc
    19261 tgccttcgcc ttatggcatt cgcctgatca aaggcagcca tattgtggtg ccgcgcgtgc
    19321 atacccaaaa gcaggcctac attctgcaaa acgaagataa acgcattgtg ttcgtgatcc
    19381 cgtggatgga cgagttttcc atcatcggca ccaccgatgt ggaatacaaa ggtgatccga
    19441 aagcagtgaa gattgaagag agtgaaatca attacctgct gaaagtgtat aacgcgcact
    19501 ttaaaaagca gctaagccgt gacgatatcg tctggaccta ctccggtgtg cgtccgctgt
    19561 gtgatgatga gtccgactcg ccgcaagcca ttacccgtga ttacaccctt gatatccatg
    19621 atgaaaacgg caaagcaccg ctgctgtcgg tattcggcgg taagctgacc acctaccgaa
    19681 aactggcaga acatgcgctg gaaaaactga cgccgtatta tcagggtatt ggcccggcgt
    19741 ggacgaaaga gagcgtgctg ccgggtggtg ccattgaagg cgaccgcgac gattacgccg
    19801 ctcgcctgcg ccgccgttac ccgttcctga ctgaatcgct ggcacgtcac tacacgcgca
    19861 cttacggcag caacagcgag ctgctgctcg gcaatgcggg aacggtaagc gatctcgggg
    19921 aagatttcgg tcatgagttc tacgaagcgg aactgaaata cctggtggat cacgaatggg
    19981 tccgccgcac cgacgatgcc ctgtggcgtc gcacaaaaca aggcatgtgg ctgaatgcgg
    20041 atcaacaatc tcgtgtgagt cagtggctgg tggaatatac gcagcagaag ttgtcgctgg
    20101 cgtcgtaaat taacgtaagg tgatcaggtc agatttcaat ctggcctgag actgatgaca
    20161 aacacaaaac tgcctgatgc gctacgctta tcaggcctac gtggtttatg caatatattg
    20221 aatttgcatg gtcttgtagg ccagataaga cgttcacgtc gcatccggca tgaacaaagc
    20281 gcactttgtc aaaaatctaa cctacttttt aattcaggga attaccgcaa agcccacgcc
    20341 atcataccca acgtaacaaa actcaggcac gttccccact cgccccgaga aaaatagcat
    20401 taatgcgccc agcgtcagca taaaaatatt gagcagaagg taaattgttg gcgtaaagag
    20461 taaattgcaa acctcaggaa tagcggcaag cccagccccc agcatggcaa taaccgctgc
    20521 aaaaaattgc gtcagtattg ggatgccgaa aaaagtcatt accagcgagg tcaaaatcca
    20581 ttttctattt tgcattattc tttccattct ttctgaatgg cgaaattata ctccccgagt
    20641 ccccttgccc cttctggaca cttttccgaa atgatggcga aaaaaacggg accctttgg
//
LOCUS       DAEEGA010000072        20571 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEEGA010000072 DAEEGA010000000
VERSION     DAEEGA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20571
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.072"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            409..1239
                     /locus_tag="KLH46_002837"
     CDS             409..1239
                     /locus_tag="KLH46_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBE0379452.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="KLH46_002838"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="KLH46_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBE0379453.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="KLH46_002839"
     CDS             complement(2368..3828)
                     /locus_tag="KLH46_002839"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBE0379454.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="KLH46_002840"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="KLH46_002840"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBE0379455.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="KLH46_002841"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="KLH46_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBE0379456.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="KLH46_002842"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="KLH46_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBE0379457.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="KLH46_002843"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="KLH46_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBE0379458.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="KLH46_002844"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="KLH46_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBE0379459.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="KLH46_002845"
     CDS             complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="KLH46_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HBE0379460.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIYGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="KLH46_002846"
                     /pseudo
     CDS             complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="KLH46_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
     gene            complement(12470..13009)
                     /locus_tag="KLH46_002847"
     CDS             complement(12470..13009)
                     /locus_tag="KLH46_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE0379461.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="KLH46_002848"
     CDS             complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="KLH46_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBE0379462.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(14103..14699)
                     /gene="fimE"
                     /locus_tag="KLH46_002849"
     CDS             complement(14103..14699)
                     /gene="fimE"
                     /locus_tag="KLH46_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HBE0379463.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GNRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(15177..15779)
                     /gene="fimB"
                     /locus_tag="KLH46_002850"
                     /pseudo
     CDS             complement(15177..15779)
                     /gene="fimB"
                     /locus_tag="KLH46_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            17233..17949
                     /gene="nanC"
                     /locus_tag="KLH46_002851"
     CDS             17233..17949
                     /gene="nanC"
                     /locus_tag="KLH46_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBE0379464.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            17969..19075
                     /gene="nanM"
                     /locus_tag="KLH46_002852"
     CDS             17969..19075
                     /gene="nanM"
                     /locus_tag="KLH46_002852"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBE0379465.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            19140..20120
                     /locus_tag="KLH46_002853"
     CDS             19140..20120
                     /locus_tag="KLH46_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBE0379466.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPTCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         5478 a         4908 c         4673 g         5512 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gaatgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg tagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc atcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaatc gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtaga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
    11101 cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
    11161 ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcttggacca
    11221 ttgtggttaa tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg
    11281 tattcagccc catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
    11341 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
    11401 cgacgcgata cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca
    11461 cagcctgggg atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag
    11521 gtgcctgtgc ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat
    11581 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
    11641 ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc
    11701 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    11761 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
    11821 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt
    11881 aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
    11941 ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc
    12001 tgataattgc gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg
    12061 ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat
    12121 caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga
    12181 taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac
    12241 tattttcatc attatttgtc acggcaagtt gcacttgttt ttaccctgcc ggataaatta
    12301 cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga
    12361 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    12421 ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg
    12481 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    12541 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg
    12601 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg
    12661 aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac
    12721 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    12781 caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc
    12841 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
    12901 cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag
    12961 caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta
    13021 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga
    13081 tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca
    13141 taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta
    13201 aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ccaggatctg cacgccaacg
    13261 tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca
    13321 attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc
    13381 agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta
    13441 cgaacctgtc ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg
    13501 ttaacaactt cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga
    13561 gccgctgcgg aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc
    13621 atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
    13681 ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca
    13741 tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg
    13801 ttggcttaat attctattat tatctttatt tatagatttt tatattgcat gaggtagttt
    13861 ttggagagaa gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg
    13921 gaggtgatgt gaaattaatt tacaagagaa ataatttaca tatcaaatag ttagatgctt
    13981 tttgtcgttt tttaatattt ttatgcttga gaaaaaatac gtaacttatt tatgatatgg
    14041 acagtttggc cccaattgtc ttgtgtttat ttgtttttta aatttattat caataagtta
    14101 aatcaaacct cttctctttt taatttttcg tttatgagat tatttctttc ccataatccg
    14161 gcaaaacgag cagcattact ggcggtataa cgcacagtat ggcgaatatt tcgattcccg
    14221 agataatcct gaattaaacg ggtatctgca ccacgctccg ccagttcata accgcaagca
    14281 tgccttaaca tatgaggatg agtctgcgtt acggttccag cttcaatacc ggcatcgcga
    14341 ataatgcgat aggcctgctg gcgagaaagc cgactcccgc ggcgagaaat aaatatagcg
    14401 tcggtccggt cagcgccttt ccagttagca cgttcctggg tccagcgttc cacggcttca
    14461 cgctcatcaa aacgtaacgg gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt
    14521 attcgacctt cattaaggtc aaggtcctga taatgcagat caagcagttc actaatacgc
    14581 atcccatgcc gatatgccaa cagaataaga caataatctc tggctcccgt tgccccgtaa
    14641 caaaccgcct gcatcatggc ctgaacttct ttaccggtaa gataacgacg tttactcaca
    14701 atagtagtac tcctaactga gatatatttg aatgaatacc tataggaaac ctcaatcggt
    14761 caaaattagc ccaatagata aaaagataac cacacacgaa gttatgtggc tatcagtcaa
    14821 ttacattaaa catcatacaa aaataaaata tcactgatgt gacaacatct attttcattg
    14881 atttgattca atataacaat agattacaga ggattaactt ccagttactt aaaaaataaa
    14941 gaaaaaagtg tggaaacagg agaatttaag tgatgtgtaa acttatgaat gattttgttt
    15001 atttattttg aagaacataa accattagat tttgtatggg tatggttaac ttaatgttta
    15061 ttatgataat taagaatcac acttacaata acatcaatta ataaatggtt aatcattttt
    15121 gatatatcgt aagaataatg tagtttttaa caccatccct ggtatctcaa ctatctctat
    15181 aaaacagcat gacgctgtcg tcctctggct ctatcccaga tgccgtaaaa acgccctgca
    15241 ttgctggcgg tataccagac agtatgacga atattgcgat gcccaagata atcctagata
    15301 agtcgcgtat ctattcccat attcgccaaa gcaaaaccac acgaatagcg taacatgtgc
    15361 ggatgaatct ccagtgacaa cccggcatta ccaccagaag tcgagataat atggtaaaac
    15421 tgttgccgag aaagcggatt ccccttacgt gataaaaata cccactcgct ctcagcatgc
    15481 ggataagaag tacggatact caaccagttt tttaaagcct gaacttcttt gttcaatagc
    15541 gggtgcgttg ttgaaaagcc tttttttaat cgatggatat atatacactt tgctttaaga
    15601 tcaatatccg aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa
    15661 agcaaaatca gacaataatt acgtgttgca tgaggcccgg tatttgctgc tttaaggagt
    15721 gactcgattt cactatgggt aaggaagttc ctttttttgt tatcagcctt attcctcatc
    15781 gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt
    15841 tttatcctga ttatgtttat aaaagcgaac gtttgcttaa taacttacgt aagtggacca
    15901 gttcttctga gcgaacttaa atggagtagc gatagttact tataataact ttgccataca
    15961 aacgccatgc tcttgcatgc tatgtacctt tatatattta tcaatctgat acgaatctaa
    16021 taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta
    16081 acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttaaac tagccgtctc
    16141 aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt
    16201 aatttcttag tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg
    16261 ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat atgggggaac
    16321 gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta
    16381 agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc
    16441 atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac
    16501 aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgagta
    16561 aacccttatt ttcaccactc tcactgagtt agcttgcaaa gctttctttg tttagattct
    16621 aaataaatta tttataaaac atatagttaa aagaaaatct ttagtttttg aagaccaccg
    16681 caagtgttcg tctggcttca catggcatct tcttcttaga aaaagatcga catattttgt
    16741 gacgcgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat ctggtataac
    16801 aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac
    16861 atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga
    16921 aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg agtaagtgat
    16981 ataacttacg aatattcaaa tcaattaaaa atcagaagag attttatact caggtattta
    17041 atctggatct ctgtttattt aaataatgtt aaaagagaat tttcacagga gaccttatac
    17101 aaaaaatata aaatacagtt acccgttgcc aaagacacta taagcctggc aaaaaaatat
    17161 tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa
    17221 tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg
    17281 tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc
    17341 cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc
    17401 atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc cgcactcaat
    17461 gatgttcagg ttgaagttaa ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg
    17521 ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt aaaactgtcc
    17581 tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg gaaagcttac
    17641 cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg ggatggatat
    17701 gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct atacagcaaa
    17761 cagaatgatt atcgctatgc aaaccacaag aaatgggcga cggaaaatgc ctttgttcta
    17821 caataccata tgacgcccga tattacgcca tacatagaat atgactacct tgaccgccag
    17881 ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg tgtgtcattt
    17941 aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc ttgctatcat
    18001 gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc catttaaaag
    18061 tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg caggtacagc
    18121 atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag ctgcattccc
    18181 tggtggaccg agagagcaag caacctctgc atttattgat ggcaatctgt atgtgtttgg
    18241 cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac acaaatacaa
    18301 ccccaaaacc aatagctggg ttaaattgat gtcgcacgcg ccgatgggca tggcgggtca
    18361 tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaatatctt
    18421 caatggctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta tagataaaat
    18481 caacgcccac tattttgaca aaaaagcaga agattatttc ttcaataagt ttctgttgtc
    18541 ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt acggaacggc
    18601 tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg aagccaaacc
    18661 aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt taaaatggaa
    18721 taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg ggataagcaa
    18781 tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa attaccagaa
    18841 cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata ttcatctttg
    18901 gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct acggagtatc
    18961 attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg gcaaagcggt
    19021 gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa actaacgctt
    19081 cagggccccg gtaaaggggc cctgctatca atttgtcatt tgtattacgc gaaaattata
    19141 tgaacgcaat aatatcaccc gattattact atgttcttac cgttgctggt cagtctaatg
    19201 ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct cattccagaa
    19261 ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgacatgt cactttaacg
    19321 acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt tatcaccatc
    19381 ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg catattgcac
    19441 ggaaattact gcccttcatt cctgataatg cagggattct tatcgttccg tgttgccgtg
    19501 gcggatcggc ttttaccgcg ggcagcgaag ggacatattc agaacggcac ggagccagcc
    19561 atgatgcttg tcgttgggga acggatactc cgctgtacca ggatttagtc agcagaacgc
    19621 gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg atgcaaggcg
    19681 aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat catatggttg
    19741 aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt actgacgcgc
    19801 cgtggttttg cggcgatacc acctggtact ggaaagaaaa tttcccgcat tcgtatgaag
    19861 ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc gacttccagc
    19921 aacaaggtga aagaggactg acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg
    19981 gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg cgaagcagtc
    20041 atttcagcac ggcagcccgt cgggggatta tttctgacag atttgtagaa gcaattttgc
    20101 agttttggcg cgaaaggtga gcggggagtt tattcatggg ggatgtcctt gttcgttctc
    20161 agagtagaca acaaaaaaca aatgagatca aggtaatatc tcttcacttg cgggctcgaa
    20221 cagcaggcaa ttgccaatat aataatctgg cacaagggtg aaaaacgcgc accgcataaa
    20281 ccgttgttat tgcttacaga ttagccggat gccttaatag acatccgcgc cttttcgatt
    20341 atggctcgga aatctacgaa catctacaca gtttactgga acgttttggg ccacagcgtt
    20401 cgcagcaccg tcctgatatg ccgttctggc gattataagt cgacggattc tggcaaatag
    20461 acgatgatga tatgcttaat gatgttcaaa gaaattaggc cattattata ttattgcttg
    20521 cattttcttt cattcctaaa agcacccgcc attaatccca ggcgcattat g
//
LOCUS       DAEEGA010000073        20521 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEEGA010000073 DAEEGA010000000
VERSION     DAEEGA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.073"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="KLH46_002854"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="KLH46_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBE0379467.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="KLH46_002855"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="KLH46_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HBE0379468.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="KLH46_002856"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="KLH46_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBE0379469.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="KLH46_002857"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="KLH46_002857"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBE0379470.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIHVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="KLH46_002858"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="KLH46_002858"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBE0379471.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="KLH46_002859"
     CDS             4449..4814
                     /locus_tag="KLH46_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBE0379472.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="KLH46_002860"
     CDS             4964..5482
                     /locus_tag="KLH46_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBE0379473.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="KLH46_002861"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="KLH46_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="KLH46_002862"
     CDS             6713..7195
                     /locus_tag="KLH46_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBE0379474.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="KLH46_002863"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="KLH46_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBE0379475.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="KLH46_002864"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="KLH46_002864"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBE0379476.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="KLH46_002865"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="KLH46_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBE0379477.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="KLH46_002866"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="KLH46_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBE0379478.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="KLH46_002867"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="KLH46_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBE0379479.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="KLH46_002868"
     CDS             10939..11730
                     /locus_tag="KLH46_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBE0379480.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="KLH46_002869"
     CDS             11751..13037
                     /locus_tag="KLH46_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBE0379481.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="KLH46_002870"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="KLH46_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBE0379482.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="KLH46_002871"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="KLH46_002871"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBE0379483.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="KLH46_002872"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="KLH46_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBE0379484.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="KLH46_002873"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="KLH46_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBE0379485.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="KLH46_002874"
     CDS             complement(16985..17275)
                     /locus_tag="KLH46_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBE0379486.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="KLH46_002875"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="KLH46_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBE0379487.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="KLH46_002876"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="KLH46_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBE0379488.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="KLH46_002877"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="KLH46_002877"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="KLH46_002878"
     CDS             19137..20378
                     /locus_tag="KLH46_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBE0379489.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRHAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5304 a         5358 c         4812 g         5047 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctagaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacatgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacagattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acagacaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acattccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttaccta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcatg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DAEEGA010000074        20417 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEEGA010000074 DAEEGA010000000
VERSION     DAEEGA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20417
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.074"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(60..818)
                     /gene="aroD"
                     /locus_tag="KLH46_002879"
     CDS             complement(60..818)
                     /gene="aroD"
                     /locus_tag="KLH46_002879"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBE0379490.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="KLH46_002880"
     CDS             complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="KLH46_002880"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBE0379491.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLVCEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1727..2998)
                     /locus_tag="KLH46_002881"
     CDS             complement(1727..2998)
                     /locus_tag="KLH46_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0379492.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVQFGERKFSTRLNTIKHRG"
     gene            complement(3181..4386)
                     /locus_tag="KLH46_002882"
     CDS             complement(3181..4386)
                     /locus_tag="KLH46_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0379493.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4486..4842)
                     /locus_tag="KLH46_002883"
     CDS             complement(4486..4842)
                     /locus_tag="KLH46_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBE0379494.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4978..5085)
                     /gene="rprA"
                     /locus_tag="KLH46_002884"
     ncRNA           complement(4978..5085)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="KLH46_002884"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="KLH46_002885"
     CDS             complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="KLH46_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBE0379495.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6772..9828
                     /locus_tag="KLH46_002886"
     CDS             6772..9828
                     /locus_tag="KLH46_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBE0379496.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVEGIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9825..10235
                     /gene="menI"
                     /locus_tag="KLH46_002887"
     CDS             9825..10235
                     /gene="menI"
                     /locus_tag="KLH46_002887"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBE0379497.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10335..10523
                     /locus_tag="KLH46_002888"
     CDS             10335..10523
                     /locus_tag="KLH46_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBE0379498.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11071..11439
                     /gene="sufA"
                     /locus_tag="KLH46_002889"
     CDS             11071..11439
                     /gene="sufA"
                     /locus_tag="KLH46_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBE0379499.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11448..12935
                     /gene="sufB"
                     /locus_tag="KLH46_002890"
     CDS             11448..12935
                     /gene="sufB"
                     /locus_tag="KLH46_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBE0379500.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12945..13691
                     /gene="sufC"
                     /locus_tag="KLH46_002891"
     CDS             12945..13691
                     /gene="sufC"
                     /locus_tag="KLH46_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBE0379501.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13666..14937
                     /gene="sufD"
                     /locus_tag="KLH46_002892"
     CDS             13666..14937
                     /gene="sufD"
                     /locus_tag="KLH46_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBE0379502.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDSLALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14934..16154
                     /gene="sufS"
                     /locus_tag="KLH46_002893"
     CDS             14934..16154
                     /gene="sufS"
                     /locus_tag="KLH46_002893"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBE0379503.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16167..16583
                     /gene="sufE"
                     /locus_tag="KLH46_002894"
     CDS             16167..16583
                     /gene="sufE"
                     /locus_tag="KLH46_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBE0379504.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16732..17736
                     /gene="ldtE"
                     /locus_tag="KLH46_002895"
     CDS             16732..17736
                     /gene="ldtE"
                     /locus_tag="KLH46_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBE0379505.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17799..18035)
                     /gene="lpp"
                     /locus_tag="KLH46_002896"
     CDS             complement(17799..18035)
                     /gene="lpp"
                     /locus_tag="KLH46_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBE0379506.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="KLH46_002897"
     CDS             complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="KLH46_002897"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBE0379507.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(20307..>20417)
                     /locus_tag="KLH46_002898"
     CDS             complement(20307..>20417)
                     /locus_tag="KLH46_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0379508.1"
                     /translation="LRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         5344 a         4888 c         5204 g         4981 t
ORIGIN      
        1 ggttaaaaat acttttttta tttcggcggg ggagggtatt tccgccgaaa tattattgct
       61 tatgcctgat gtaaaatagt taataccgtg cgcaaatcat ttaccgagat ttgccctggc
      121 gcagaggctt tttttaccgc accaaaagtt gccgccgagc caaatacttc accaaccaga
      181 cgagaaatta cgccagtttt tgccatcgac atcgtgatga ttggacgatc ggcatactgc
      241 tcctgcatct ccagggtcgc ggcaagcaac gtcagcacat cgctggtact ttgcggcatc
      301 agcgcaatct taggaatatc ggcgtcgaag gactgcattt tgcgcagacg ggcaatgatt
      361 tcttcggctt ccggcgtttt atggaagtca tggttggaca tgacaacttt cacatcatgc
      421 gcgtgggcgt aggcgacggt ttctttgacc tgatcatcgc cggtaaataa ctccagatcg
      481 atcatatcaa ccaggccgct gtcgatggct gcacgattga gagcaatata agcctcggtg
      541 gaaatcgcct gctcgccgcc ttctttggca ctgcggaagg taaacagcag cggtttttct
      601 ggcatggttt cacggagaat ttttgccgcc gccatgacag actccacatt ggagaggtcg
      661 gcaaagtggt ccacacgcca ttccagaata tcaaagtccg cttcacgata ggcgagagct
      721 tcggatttca cgcgggcgat atctttcgcc atcagcgaga cgatgatttt aggtgcgccc
      781 gcaccaatga cgagatcttt tacagttacg gttttcattt tttacccttt ctgcacgcgg
      841 tcagcctgtc aggcaccgaa ccccatgacc tgtttaacat attccagagg gaaatctttg
      901 ccagtccata acgtgaactg ttcagcccct tgccacaaca acatgccgta tccatcaatc
      961 gttttgcaac cagcttgttg tgcctgctgc aataacttcg tcatatgcgg gttatacacg
     1021 cattcagtga ccagcagtcc cggatgtaac agactgatat cattaaccaa tgattcattc
     1081 tcaaggggtt tcatacctac ttttgtgcca ttggttaaaa tgtcggcgga agccagggct
     1141 tcagcaaagg cttgctgatc ggcgagatcg gtatccgtga cgacacaatc ggtgttttcg
     1201 ttaacccgct gcgcgaaggc gagggcttta tcgaagaact catcccgacg gttaaagagt
     1261 ttaatttctt ttaaaccttc aattgccccc tgcgcgccaa tcgccgttga ggcacctccg
     1321 gcccctaaca gcaccatcgt tttgcctttg atatcaaaac cgctctcttt aatggcgcga
     1381 atatggcccg tgccgtcggt gttatagcca cgcagatagc catcatcatt aacgatggtg
     1441 ttgatggcac ccaccagttt ggctgctggc gttaattcat caacatattc acacaccagt
     1501 tgtttgttcg gcatcgacac accagttccg cgcattttga gggcttttaa tccttcaatt
     1561 gctccgggaa agctatcgtt atccacttcg aaggccatat aggtaaatgg caatcccgct
     1621 ttttctaagg ctttattctg catttcaggc gataaactgt ggcggatagg ataagccatc
     1681 aatccaatca attcgtattt tgcggtaaca tccataatta actcctttaa cctctatgct
     1741 taattgtgtt taaccgggtg ctaaatttgc gttcgccaaa ctgcacatca ttctcaggaa
     1801 taataaaaac gcggtaatag cggctaaaaa caataattgc ggtaatcagg gccagcagcg
     1861 cgaaagtaaa atcgagaaca atgatatatt gcaggccgat gttcgacaga taaccggtaa
     1921 tcagcggaat aacaaagtta gccagtccac ccatcatcat ataaatactg gtgactttgg
     1981 ctttgctttt ggggaaaaac tctgacatga ccgaaacacc gagctgtaaa atgccgccag
     2041 ctgctgagaa accgataaca aaggcgccgg cattacacac cagtggagaa gggtacaggt
     2101 agataatggc agctgttatt gttgccagcg cagagttaaa tacattagcc cagatgggcc
     2161 ggaccatttt tttcagtagt gcggcaaaaa taaagacaca gaccaacgag cccatactgt
     2221 aataagagat ggtttttaat gcctcagctt ctgacatacc agcaaaagcc atcgcatatt
     2281 tgggcatcca caccacaatc acataaaagg tcgagaatgc ggctacaccg aacagtaccg
     2341 atgaaacacc ttccagccag actaaaggtt tgctgttcat ttgcggtaat tcattagcta
     2401 cgctggcgtc caccaactgg ctggggaatt tgcttttcaa cagcatcagc gtgatcagta
     2461 caaacagaat acccggaata atcagcccat agccgtacca gatattattg agcaacatat
     2521 agctcaccag cattgggtag aacatttgcc caaatgacac catcgcttta accagtatga
     2581 ccgccgaacc agaggctttc ggaaagcatt ccatgagcgc ggggtagcca cccgtatcca
     2641 gcgctgagtt agcgatacct acgcacactg ccagaccgta ggcgagagtt aaattcgggc
     2701 aagcgggaat accaaagaag aatagcagat acattattac tgccattaat atcaccgccc
     2761 gacgaccaaa cttatcggag atcacaccga agaataaaat actgatcaat cgccccaaac
     2821 cgataccgga aattaaatag gcaatgcccg cgttgtcagt ggaaaacttt tctgccagag
     2881 atgtcatatt ttgggcaagc gtaataacac taatgccgtg caggaaatag ctgaagtaaa
     2941 tacaaagaac agccaggata aatggcgtgc tgaaagataa tttcgttacc gtacgtattg
     3001 ttctccgacg gtttacataa ttactatact gaagatgatt ttccctgggt acatggcata
     3061 aagtttatgt ttccaggaat attgcatgtc atagtttgtt tttgatcgcg attttcctta
     3121 gttagcgcag aaagagttat ttatccgtaa gatcttgcat gacctgaaaa gaatattgca
     3181 ttacccaccc ggagcgacat tttcctttag cgagtgatgc cgcgtttttt tgcggctgcg
     3241 taagccgata aacagtgcca gtaaaaaacc gatggcagta tcgaaccaca taatatcggc
     3301 aatacttctt tgggacagat gagccgtaat caacggaata gtaaaggtcg caatactgcc
     3361 cgtactgtaa tagatccctg tagctttacc tttagcgtaa gggaaacgtt cagccattaa
     3421 cgtcaggcca atttgcacaa cacctccagc agaggtaaaa ccaatgacaa aagcaaatat
     3481 tatcaccaca taaaatgtgg gatgcaggca gacggtaaac agagcaataa atgagataaa
     3541 ggtgtacagc atcagtaatg ttgttgggcg aacggtatta cgaatgagtg gagcggtaat
     3601 aaatacacaa agcagcgaac ccacggtgta gatactgagt agtttgatcg acatagtgta
     3661 tgacatgcct gcaacaaatt gtccgtactg tgccagccac tggctaacca gataaaacgt
     3721 tgccattgag atatagccat ataaggtata actggctaaa tcgataattg aacagcgatg
     3781 ttccgtagag ctggtggttt tctttatgac aggtaagcga cgacccggat ggggtgggaa
     3841 cgtacaacgg tataaaaaca gagcgttaat aaacataatg cctgcagcaa tcataaagga
     3901 ccaaccgaac cacagttcag cccacactaa caggctaatg attagcggta ataaaaattg
     3961 tccgctggaa acaaatgctt taattaaaat attggctgtc ccaggtgagc gtggaaaagc
     4021 ttccatcaaa ctgggataag tgcctgcatc gagaaaactg tttgccattc ccgccagaaa
     4081 gccaaaaaca taagcgataa tgatgttatt ggtctgcagg atgtcaaaaa agaaggccat
     4141 atagcagcac atcccgagca tgataaaagg gcggcgacca aagcgatcgg ataataatcc
     4201 tgcaaaaagc aagacactta atcgaccaat gcccagcgat gagataacta tcgagacacc
     4261 cgcggcatta gtctgccaaa gtgtctccag cgaggccata ttcaggctca tcaaaatgac
     4321 gcccatacca tgcaccaggt aattaaaata caacccaagt gcggtagggt aatagggatt
     4381 tttcataggt tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc
     4441 cattcattat tgttgtaaca acgacaattc ctttctgtgg aaaaattaac aaagccgctc
     4501 cagatcgtgt gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt
     4561 gccatcaggg tagacttgtt gaaacgcggt taagtcgggg aaaaaacgca tggtgttgtt
     4621 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc gttgctgacg
     4681 catagccaac agttgctcga ctacacaatc aggaatggcg catttgccat tttcccactg
     4741 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
     4801 ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
     4861 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
     4921 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta
     4981 aaaaaaagcc catcgtggga gatgggaaaa gactacacac agcaattcgt tgtttcactc
     5041 aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggc
     5101 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
     5161 cgacaggtac taccagctga atggcggtaa gcaggaaaat atggggaaga gcaagcaaat
     5221 atcaattagc ggtgattaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc
     5281 ggtttctcga tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggtggcggg
     5341 acttcttcca cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata
     5401 aacagaccga tcatcccgaa agcaatcaaa ccaccaataa cgccagagag gatcaggatc
     5461 agcggtaaat cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca
     5521 accacaccgc tccacactaa caataccgtt ccccaggtgg tatcgccggt ccagtagagc
     5581 cagataatcg ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc
     5641 attaacaccg ttagcaacgt tgcataaggt acgccggata cggcgaggcc gataccgcca
     5701 agcactgcct gtactaacgc cgtcaccacc acacccagcg ccaccgcgcg gatagcctgt
     5761 gccgccagca gcacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga
     5821 atgccttgtg ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc
     5881 gcacaatgca ccataaagcg cccgatatgc gccgcctgcc caacgaacca ggtggtggtg
     5941 gtgccaatat aagggcggac tttcgccatg atcgccgtac cccccatatc cagcaagttg
     6001 tgccagcctg catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg
     6061 ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata
     6121 ctgttaacca gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc
     6181 agaacggcga gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag
     6241 gtggcgataa ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac
     6301 agacatgcca caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc
     6361 ctgggctgac gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga
     6421 cgcgacgtga acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac
     6481 aggccgattc tgcccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc
     6541 aggcaacacg caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc
     6601 gttcacccca gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg
     6661 caactcgaaa tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg
     6721 aaagaatagt gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
     6781 cagatttccc tggcacccgg tgtcgttcaa ctggtgctta attttttgca agagctggag
     6841 caacaaggtt ttaccggcga tgcggcgaca agttatgccg atcgtctgac aatgtcgacc
     6901 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
     6961 gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
     7021 cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
     7081 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
     7141 gccggggtga taaaagacca actcaatcag tacctgaaac cgttcggcta cttttttgcg
     7201 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc
     7261 ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt acgcgcggtg
     7321 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgagctggc ggaaacgctg
     7381 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
     7441 caacgccagt taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat
     7501 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
     7561 tcagaaggga cgctggcctt tattaccgaa gcgcggctgg atattacgcg cttgcctaaa
     7621 gtgcgccgtc tggtgaatgt caaatatgac tcttttgact ctgcgctgcg taacgcgccg
     7681 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
     7741 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa
     7801 gagatgctcg gactgaacat tgtggaattt gctggtgatg atgaggcgct gattgacgag
     7861 cgagttaatg cactctgtgt gcggcttgat gagctgattg ccagccaaca ggcaggtgtg
     7921 attggctggc aggtgtgccg cgagctggcg ggggttgaag gtatctatgc gatgcgcaaa
     7981 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
     8041 gatacctgcg taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc
     8101 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc
     8161 cgtccggcac tggatatgtg cgatccgcaa caagagattt tgatgaagca aatctctgat
     8221 gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt
     8281 tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
     8341 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
     8401 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
     8461 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
     8521 tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg
     8581 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgctt gttggcggat
     8641 cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
     8701 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca
     8761 cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcttgttc gacccagtgc
     8821 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
     8881 tatttacgcc cgcttcgcga ccacctcgtt gctacggtcg agagctacgc accgctgatg
     8941 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg
     9001 gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg tcccctcgct acaacaacaa
     9061 atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaagctct caatgcagag
     9121 cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
     9181 gtggtggcgg attttgtccg tctggttgaa aaattaggtt tccagcctgt gttactgcca
     9241 ttttcgccaa atggcaaagc ccagcatatc aaaggctttc ttaatcgttt tgcgaagacg
     9301 gcgaaaaaga cggcggattt cctcaaccgt atggctaagc ttgggatgcc aatggtgggc
     9361 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
     9421 cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg caagcgcact tgagtcacat
     9481 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaggttacc
     9541 gctttgccgg gcgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg
     9601 gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac
     9661 catgaaaatt cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg
     9721 cgtaaccgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc
     9781 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgga
     9841 aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa catggtgggg ttcctggata
     9901 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
     9961 caaagcagcc tttcgggttg ctgcatggag gagcatccgt ggtactggcc gaaagtatcg
    10021 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca
    10081 atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg cggcatatgt aaaccgttgc
    10141 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
    10201 gctgttcgtc acgattgacg actgccattt tgtgacgttg tcaccagaaa agtgtgacgc
    10261 atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
    10321 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
    10381 gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
    10441 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg
    10501 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg
    10561 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt
    10621 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
    10681 ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg
    10741 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    10801 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    10861 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
    10921 cctgttaacc cacacatcag gggctatgct tattaaaagc aggcaaaaag aatgttttac
    10981 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat
    11041 acgctgaaag cgatgaagtg aggtaaatag atggacatgc attcaggaac ctttaaccca
    11101 caggatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgcgag
    11161 ctggtggcaa agcagcctgg tatggtcggc gtgcgcttag gtgtgaagca aacgggctgc
    11221 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
    11281 gaacacgacg gcgcgaagct gtttgtcccg ctgcaatcga tgccgtttat tgatggcacg
    11341 gaagtcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
    11401 cagaatgaat gtggctgtgg cgaaagcttt agggtatagg cggtactatg tctcgtaata
    11461 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
    11521 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
    11581 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc
    11641 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
    11701 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
    11761 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
    11821 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
    11881 ttttcgactc tgtttcggtt gccaccactt atcgcgaaaa actggcggag cagggaatta
    11941 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
    12001 gcaccgtggt gccggggaat gacaacttct ttgcagcact taatgcggcg gtagcctctg
    12061 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
    12121 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac aattctggtg gccgacgaag
    12181 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttgc
    12241 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
    12301 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
    12361 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga
    12421 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
    12481 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
    12541 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
    12601 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
    12661 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
    12721 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
    12781 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
    12841 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
    12901 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
    12961 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc
    13021 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtacc ttatcggcaa
    13081 cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
    13141 atttgcttgc actgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt
    13201 atccggtgga gattccaggc gtcagtaacc agtttttcct gcaaacggca cttaatgcag
    13261 tgcgcagcta tcgcggccag gaaacgctcg accgctttga ttttcaggat ttgatggaag
    13321 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
    13381 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
    13441 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
    13501 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
    13561 gcattctcga ctacatcaag cctgattacg ttcatgtgct atatcaggga cgaattgtga
    13621 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
    13681 aacagcagta atgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
    13741 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
    13801 catgaaaact ggaaatatac gccgctggaa gggctgacca atagtcagtt tgtcagcatt
    13861 gcgggagata tatccccaca gcagcgtgat tccttagcgt taacgttaga cgccgtgcgg
    13921 ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg atgcaattga aggcagcgga
    13981 tatgaagtga gcattaacga cgaccgtcag gggttacccg acgctattca ggcggaagtg
    14041 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
    14101 caacggccgg caaagccatt gctgttaatg catatcaccc agggcgtggc aggtgaagag
    14161 gtgaacactg cccattaccg gcatcatctg gatctggcgg aaggtgccga agcaacggtg
    14221 atcgaacatt ttgtcagcct taatgatgct cgtcatttta ccggggcacg gttcactatc
    14281 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
    14341 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
    14401 ttcctgctgg gtggcgcagt gttacgacat aacaccagta cgcaactcaa tggcgaaaac
    14461 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
    14521 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
    14581 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    14641 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcgga agtggataca
    14701 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    14761 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
    14821 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
    14881 cttaaacagc aggtgctggc ccgaatcggt caacggctgc caggaggtgc aagatgactt
    14941 tttccgtcga caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc
    15001 cgctggctta tctcgacagc gccgccagtg cacagaaacc gagccaggtg attgacgccg
    15061 aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    15121 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    15181 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
    15241 atagctgggg caacagcaat gtgcgggcgg gcgataacat catcatcagt cagatggagc
    15301 accacgctaa cattgtcccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    15361 tgatccctct caaccccgac ggtacgctgc aactggagac gctgcctacg ctgtttgatg
    15421 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatccac
    15481 tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc
    15541 aggcggtgat gcatcatccg gtggatgttc aggcgctgga ttgcgacttt tacgtgttct
    15601 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
    15661 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    15721 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg
    15781 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aatagcatag
    15841 ccgagtatga acagaatctg atgcattatg cgctatcaca gctggaatct gtaccggatc
    15901 tcactctcta tggccaacaa aacaggcttg gcgttattgc ttttaatctc ggtaaacacc
    15961 acgcctatga tgttggcagt tttctcgata attacggcat tgctgtgcgt accggacatc
    16021 actgcgctat gccattaatg gcctattaca acgtccctgc gatgtgtcgg gcgtcgctgg
    16081 ccatgtataa cacccatgaa gaagtggagc gtctggtgac cggcctgcaa cgtattcacc
    16141 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
    16201 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    16261 agcgtctgcc agaattacgc gccgaagacg gaagcccaca aaatagcatt cagggctgcc
    16321 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
    16381 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
    16441 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
    16501 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
    16561 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    16621 ccggatggtc aatgtttaca ctgacctgca caggagtgtt ttggtttcag gtgaacataa
    16681 gcaccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    16741 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    16801 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
    16861 gtgcaagaag gggataaaaa ccttcaggct attgcccgac gttttgatac tgcagcaatg
    16921 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact
    16981 attccttcac aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca
    17041 gagctgcgcc tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt
    17101 attggattgc aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct
    17161 aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt cgctggagcg tggcattaaa
    17221 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcctc
    17281 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgttggtttg
    17341 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
    17401 gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
    17461 aacgggatgc gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt
    17521 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    17581 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
    17641 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
    17701 ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt aaatagcaga caaaaaaatg
    17761 gcgcacaatg tgcgccattt ttcacttcac aggtactatt acttgcggta tttagtagcc
    17821 atgttgtcca gacgctggtt agcacgagct gcgtcatctt tagcagcctg aacgtcggaa
    17881 cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag cgttcagagt ctgaacgtca
    17941 gaagacagct gatcgatttt agcgttgctg gagcaacctg ccagcagagt agaacccagg
    18001 attaccgcgc ccagtaccag tttagtagct ttcattatta ataccctcta gattgagtta
    18061 atctccatgt agcgttacaa gtattacaca aagtttttta tgttgagaat atttttttga
    18121 tgggaaggca cttatttttg atcgttcgct caaagaagca tcgaacgctg ggttttagat
    18181 aaaaaaattg agagaaaaca ggatgcttcc atcggattca tcttaaataa agtgagatta
    18241 tatagtgaaa tccgatttga ttttttattg cttctggtta tcgattaaat aaaaaaagcg
    18301 cccatcaggg cgcttcgata tacaaattaa ttcacaaaag caatattaca ggacgtgaac
    18361 agatgcggtg ttagtagtgc cgctcggtac cagtgcacca gaaaccataa ctacgacgtc
    18421 acctttgtgt gccagaccgc tctgcagagc cagttcttta cccagacggt agaaatcatc
    18481 agtagaagtg atctctttaa ccagctgcgg cacaacgcct ttgctcagta ccaactgatg
    18541 agccgttttt tcgttggtgg tcagtgccag gatggtggca tccgggaagt atttacgtac
    18601 tgcgcgagca gatttaccgc cctgggtagc aaccacgatc agcggagcat ccagtttttc
    18661 agcagtttca acggcaccac ggcataccgc ttcggtgatg cgcagtttac ggttgtcatt
    18721 gttgaactcg agacggctgt tcatcacgcg gtcggtacgt tcgcagatgg tcgccatgat
    18781 agaaaccgct tccagcgggt atttaccttt tgcggattca ccagacagca tcactgcgtc
    18841 agtaccgtcg aggatggcgt ttgcaacgtc accggcttct gcgcgagtcg ggcgtgggtt
    18901 tttgatcatg gaatccagca tctgggtcgc agtgataacg actttacgtg cacggataca
    18961 tttttcgatc atcatcttct gggcgaagat aacttcttct accgggattt ctacacccag
    19021 gtcgccacgc gcaaccatga tgccgtcaga ggcttcgagg atttcgtcga agttgttgag
    19081 gccttcctgg ttttcgattt tggagatgat gtggatgttt tcgccgccgt gcgctttcag
    19141 gtgctcacgg atttcgataa cgtcagaacg cttacgaata aaggaagcag caacaaagtc
    19201 tacgcctttt tcgcaaccaa agatcaggtc ctgtttgtct ttttcagcca gtgctggcag
    19261 agcaatggaa acgccaggca ggttcacacc tttgttttcg cccaggtcac cgttgttcag
    19321 cactttacag ataactttgt taccttcaat ggcggtaact tccataccga tcagaccatc
    19381 gtcaaccagt acggtgttgc caacagacag gtcagtagtg aaaccttcat acgttaccgc
    19441 aaccatttcg ctgttgccga taacagattt atcagtggtg aaagtaaagg tctgaccagc
    19501 tttcagagaa acatcgttac cgccttccag tttcatggtg cggatttccg gacctttggt
    19561 atcaagcagg atagcggcgg ttttaccagt tttgctcatc acgttgcgca gattctgaat
    19621 gcgctgaccg tgttctgcat agtcaccatg agagaagttc agacgcataa cgttcatgcc
    19681 agcgtccagc attttagcta acatctcttc agattcggtt ttcggtccga tggtgcaaac
    19741 aattttggtc tttttcatga cagtcttagt ctttaagttg agaaggatgg gagaaacttg
    19801 ctttctgggc gcactgccgg aaagcgaatc cggtattacg aaagtggtgg tgcctcgctc
    19861 taaggatagg tgacatcgaa aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac
    19921 gtgagtgtct gtttttgagt cgttgagttc tacagtccaa agtgctgaaa ccattcaaga
    19981 gtcaattggc gcgcattata cgctgaaatt aatccaaaag gaaaacaaaa acagcgtttc
    20041 aaaaaatata caacttgtta gacaataaat tgttatcaaa gatttaacgt tccagggaaa
    20101 aggttacgca aaaacttcgc tgtttgcgca acgtctggtg ccgaagttgt cagtgtgagg
    20161 ataagtgaaa gttcatccgg tgtataaata tatcatcgct caattgctac agttaaaata
    20221 atttatcgcg ttatttgtct gattttgttt gatttttgtt taattggtta gattgatata
    20281 tgctgatagt tggtaatgac tccaacttac tgatagtgtt ttatgttcag ataatgcccg
    20341 atgaccttgt catgcagctc caccgatttt gagaacgaca gtgacttccg tcccagcctt
    20401 gccagatgtt gtctcag
//
LOCUS       DAEEGA010000075        20387 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEEGA010000075 DAEEGA010000000
VERSION     DAEEGA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20387
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.075"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            112..1167
                     /gene="phoE"
                     /locus_tag="KLH46_002899"
     CDS             112..1167
                     /gene="phoE"
                     /locus_tag="KLH46_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBE0379509.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1206..1607)
                     /gene="crl"
                     /locus_tag="KLH46_002900"
     CDS             complement(1206..1607)
                     /gene="crl"
                     /locus_tag="KLH46_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBE0379510.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="KLH46_002901"
     CDS             complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="KLH46_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBE0379511.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="KLH46_002902"
     CDS             complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="KLH46_002902"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBE0379512.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3721..5178
                     /gene="pepD"
                     /locus_tag="KLH46_002903"
     CDS             3721..5178
                     /gene="pepD"
                     /locus_tag="KLH46_002903"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBE0379513.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5235..5849)
                     /locus_tag="KLH46_002904"
                     /pseudo
     CDS             complement(5235..5849)
                     /locus_tag="KLH46_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5911..6954)
                     /locus_tag="KLH46_002905"
     CDS             complement(5911..6954)
                     /locus_tag="KLH46_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBE0379514.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            complement(7200..7652)
                     /locus_tag="KLH46_002906"
     CDS             complement(7200..7652)
                     /locus_tag="KLH46_002906"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0379515.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="KLH46_002907"
     CDS             complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="KLH46_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HBE0379516.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="KLH46_002908"
     CDS             complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="KLH46_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HBE0379517.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="KLH46_002909"
     CDS             complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="KLH46_002909"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBE0379518.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="KLH46_002910"
     CDS             complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="KLH46_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBE0379519.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10291..12003
                     /locus_tag="KLH46_002911"
                     /pseudo
     CDS             10291..12003
                     /locus_tag="KLH46_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="KLH46_002912"
     CDS             complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="KLH46_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HBE0379520.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(12893..13414)
                     /locus_tag="KLH46_002913"
                     /pseudo
     CDS             complement(12893..13414)
                     /locus_tag="KLH46_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            13798..14538
                     /locus_tag="KLH46_002914"
     CDS             13798..14538
                     /locus_tag="KLH46_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBE0379521.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(14509..15276)
                     /locus_tag="KLH46_002915"
     CDS             complement(14509..15276)
                     /locus_tag="KLH46_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBE0379522.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWHLFCLGERVV"
     gene            complement(15381..15959)
                     /gene="lpcA"
                     /locus_tag="KLH46_002916"
     CDS             complement(15381..15959)
                     /gene="lpcA"
                     /locus_tag="KLH46_002916"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBE0379523.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            16199..18643
                     /gene="fadE"
                     /locus_tag="KLH46_002917"
                     /pseudo
     CDS             16199..18643
                     /gene="fadE"
                     /locus_tag="KLH46_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
     gene            complement(18686..19132)
                     /gene="ivy"
                     /locus_tag="KLH46_002918"
     CDS             complement(18686..19132)
                     /gene="ivy"
                     /locus_tag="KLH46_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBE0379524.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            19313..20083
                     /gene="yafV"
                     /locus_tag="KLH46_002919"
     CDS             19313..20083
                     /gene="yafV"
                     /locus_tag="KLH46_002919"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBE0379525.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            20193..20387
                     /locus_tag="KLH46_002920"
                     /pseudo
     CDS             20193..20387
                     /locus_tag="KLH46_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541010.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
BASE COUNT         4985 a         5242 c         5238 g         4922 t
ORIGIN      
        1 aatatcatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
       61 acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
      121 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
      181 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
      241 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
      301 gaaactcaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
      361 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
      421 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
      481 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
      541 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc
      601 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
      661 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
      721 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
      781 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
      841 ctggcaactt tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
      901 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
      961 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     1021 aattatatcg acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     1081 tataaaatca accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc
     1141 gcggttggga tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat
     1201 ttcactcacg ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc
     1261 agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc
     1321 agtcgttcaa ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg
     1381 aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac
     1441 cagccccaga attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     1501 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     1561 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt
     1621 tgtgatgcaa ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt
     1681 tcgatccagc cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat
     1741 gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     1801 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc
     1861 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa
     1921 gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc
     1981 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     2041 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     2101 aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc
     2161 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     2221 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     2281 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg
     2341 cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc
     2401 tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc
     2461 tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac
     2521 gtagccgcat gcagccagtg tcgaccactg agtttcggat cttcttccgt acaggctttt
     2581 tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca
     2641 atcacggtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc
     2701 tggacgtcga ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc
     2761 atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg
     2821 ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac
     2881 agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac
     2941 tattgtaacc tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga
     3001 ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca
     3061 ggtatcttgc gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc
     3121 gaagatggtg acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc
     3181 ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag
     3241 cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg
     3301 acgaataccc agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc
     3361 aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat
     3421 ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt
     3481 atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg
     3541 cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga
     3601 cgcgggataa agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat
     3661 tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac
     3721 gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt
     3781 tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca
     3841 aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtaaacct
     3901 gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg
     3961 ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt
     4021 gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg
     4081 gcctctgcac tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg
     4141 ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg
     4201 caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt
     4261 gcggggggta tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt
     4321 tttgaaacct tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc
     4381 cacgttggcc tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa
     4441 gaactggacc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt
     4501 gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg
     4561 aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg
     4621 ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt
     4681 attcgtctgc tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt
     4741 gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt
     4801 cactgcctga tccgttcact gatcgacagc ggtaaagact acgtggtgag catgctggat
     4861 tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag
     4921 ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac
     4981 aagacgccga acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg
     5041 tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat
     5101 gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa
     5161 gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc
     5221 agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg
     5281 ccacgttcaa tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc
     5341 tattgctgct gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc
     5401 tggctgcgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc
     5461 gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac
     5521 cggatcgcat cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag
     5581 tttttacgcc cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac
     5641 cagctttcgc ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc
     5701 gtgtcagagt agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca
     5761 gcttctttaa ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc
     5821 tgcgctgagg agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta
     5881 gcaccggacg cagccgcgcg acaggaataa ttaatccggc ctgcccagac attgcaccac
     5941 actttcagcc gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca
     6001 aattacccga ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag
     6061 acgcccttta cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa
     6121 tgtttcctca ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat
     6181 aaccaccaga ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc
     6241 accgattcga cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc
     6301 cttaacctgt tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag
     6361 cgcgtcgtca ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa
     6421 aatagactgc cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc
     6481 cagacctgcc agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc
     6541 gaaatgattg ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc
     6601 tgacggcagg ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg
     6661 cttttcaaac ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat
     6721 gccacagccg atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc
     6781 tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat
     6841 gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc
     6901 atcagatgcg atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca
     6961 ttccgtaaat agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag
     7021 taacaattaa aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc
     7081 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
     7141 ttgtggaata agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt
     7201 taatgttgcg gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg
     7261 cgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt
     7321 gcgtccaccg taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc
     7381 ccacggcggg tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta
     7441 ataaagccga ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc
     7501 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc
     7561 tgactgacgg tcatcgtaac cgctctaatg aaaatagcgc atagttgctg aaaatcgcca
     7621 ggatgataat ctcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac
     7681 gcttctgcca tttttccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt
     7741 tcaggcttca gaatggcaat gagcaaccat gcttgctcat caaacgcacc ctgacaatat
     7801 accaaatgcg cttcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta
     7861 ttcacatttg ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac
     7921 gagtcgtcgt agagtgcatc gcgaccaaat atatctggca aaacaccgtc acgcttatag
     7981 gaaataaaat ccgccgttaa cgcatcaagt tcctctgctg taagttgcag gcgaataagt
     8041 tttgttttga atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat
     8101 cattaagata ttgtttagcg cggcgtgtaa tttcctctaa tcttgcagca cttggacgaa
     8161 agcgcgcctt tatgggcttt ttctcctctt gttcagcaag catgtccatt aacgcttcga
     8221 atgcgctggc acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaactggtt
     8281 gatcaataaa gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag
     8341 cgagaattcg atgcatacag tgataccctc ataataatgc acaccagaat atacataata
     8401 gtatacatca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     8461 gcagccccac cagacgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     8521 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgggtg gtctgctgaa
     8581 aatcgtcgaa ctttaatttc accccctggc gagcaatcag taaatcaggt tttacctttg
     8641 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     8701 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt
     8761 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8821 agcgtttaag cagaatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca
     8881 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gattttttcc agtggtaagg
     8941 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca
     9001 tgtcggaggc gattttggcg agaaacttta ctggcgccac gcccgcagat gccgtcagtt
     9061 gcagctcgtt gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     9121 aatggacgct atcggtgaca tcgagataag cctcatccag tgacaacggt tcaatgcgcg
     9181 aggtgtagcg cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc
     9241 gccccggaag caaggtgaga tgtgggcata atttgagcgc catcccagta ggcatagcgc
     9301 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacgttcgc
     9361 ggctgccgcc aatagcaata gggatatcgc gcagagcggg attgtcgcgc atctccaccg
     9421 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     9481 tggtaaagta tacagtgatt tcaggttttg agaaatgcgt aaagattcag catttagcgc
     9541 attcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     9601 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     9661 atctcaatac gccgattgcc cgcgctttgc ggattcttcg cgtccagcag catctggtcc
     9721 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     9781 cgagctgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag
     9841 gccatcgcat cggtatgccc ggtaataata attttgttat agagcgagtc gaacactggc
     9901 gcgagctcca ccagcaaagt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     9961 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
    10021 tccagattcg cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccaactcg
    10081 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct
    10141 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
    10201 attttattta acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
    10261 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc
    10321 aaagaggccg agcgtatttc cgaggtttct gcccgcttta ccctggacgc gatgcccggc
    10381 aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc gcaggcgcaa
    10441 acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
    10501 aagtttgtac gcggagacgc catcgccggg atgatgattc tggcgatcaa cctgagcggc
    10561 ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt ccagcaatac
    10621 gtgctgatga ccatcggcga cggcctggtg gcgcagatcc cttccctgct gctctccacc
    10681 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc
    10741 caccagttgc tggcaagccc gtcggtgctc tacaccgcca ccgggatcat gttcgtgctg
    10801 gcggtggtgc cggggatgcc gcatctgccg tttttgctgt tcagcgccct gcttggcttt
    10861 accggctggc ggatgagcaa acgcccgcag gcggcggagg cggaagagaa aagcctcgaa
    10921 acgctgaccc gcaccatcac tgaaaccagc gaacaacagg tcagttggga aactattccg
    10981 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcg
    11041 cagggcaacc cgctcaccca gcggattcgc ggcgttcggc aggtgatttc cgacggtaac
    11101 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gcctcaagcc cagccagtac
    11161 gctattttca tcaacggcat tgaggctgat gaagcggata ttccggcgga taaactgatg
    11221 gcgctgccct ccagcgaaac ctacggcgag attgacggcg tgctggggaa cgacccggcg
    11281 tacgggatac cggtcacctg gatccagcct gcgcagaaag cgaaggctct gaatatgggg
    11341 tatcaggtga tcgacagcgc cagtgtgatt gccacccatg tgaacaagat tgtgcgcagc
    11401 tatattcctg atttgtttaa ctatgatgac atcacgcagt tgcataaccg tttgtcgtcg
    11461 atggcaccgc gcctggcgga ggatttaagc gcggcgctca attacagcca attgctgaaa
    11521 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
    11581 gtgctggtcg ccagtagcac ggtgactaaa gatcatattc tgctggcggc cgatgtgcgc
    11641 ctggcgctgc ggcgcagcat tactcatccg ttcgttcgca agcaggagct gacggtgtat
    11701 acgctgaata atgagctgga aaatctgctg accaacgtag tgaatcaggc gcaacaggcc
    11761 ggcaaagtga tgctcgacag cgtgccggtt gacccgaata tgctcaacca gttccagagc
    11821 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagatccggt gctgctggtg
    11881 ccgccgcagc tacgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
    11941 gtgctgtcgt ataacgaagt gccggatgag ctggagttga agattatggg ggcgttgatg
    12001 taagaatccg gcatcggtgc acaattgccg gatgcggcgt aaacgcctta tccggcctac
    12061 cgttcaggca caagttttta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
    12121 cataactgcc ggatgcggcg tgaacgcctt atccggccta ccgttaaagc acgatttgta
    12181 ggccggataa gcgtaagcgc atccggcacc agcgcacagt tataaaatga tacgctcccc
    12241 ggcacttaaa tccgttatgt ctcccgccca atcgatggga tataaccctc gcgcgacatc
    12301 gcgatggaac gttgagaatg gccaatcact cacctgcttt acccagccat gtttctctgg
    12361 atttatataa atataatcaa catgatggcg ataatctttt gtattgcgaa tggcatgctc
    12421 ccaaaaacgc ggttgccaga tatttttcaa tccgcaagca tgggtgaact gctttttaat
    12481 ctcccgccag cgagaggaaa aatcatcatc gctttcgggt aatgtccata tgcaatgcat
    12541 atgctccggc aaaacgaccc aggcgttaat ttcaaaaggt ctgtctcgct taacgttaat
    12601 aatggcgtta cggagcgtct ggaactgggt ggtcagaaga tggcttcgac gattgcgtaa
    12661 atttaccgtg aaaaaccatg ttcccccctt gatgtaataa cgacgatatt ccgacatagc
    12721 gactcctttt tctcaacggt aagaggaaaa ggaaaggccg acaatgacgc tgtgttatat
    12781 cgggaaagcg gctcgcaaaa acgcacagat ttgtaggcat gataagacgc gtcagcgtcg
    12841 catcaggcat ccgtgcacaa ccgccggatg cggcgtaaac gccttatccg gcctacaaaa
    12901 tcatctcttc cgtcaaaatc ctgcgcgcgc ctaaaaaatg gtcctgccag aaaggttcgg
    12961 ctaaccggct tacccgaatg gtttccccgg tgcgcagtga ctcaataaac tgaccgtcgc
    13021 ccagatacac ccccatatgg tcggcaatct cgcggctgtg gatatggaaa aatagcaaat
    13081 ctccccggcg caggtcgtta ttcgccacaa tcgttgcccg acggtagtgg tacatctcat
    13141 tggccgtgcg cgggagtttc gcctcaagga tcttgttgta ggcataaaaa actaacccac
    13201 tacaatcgaa gccttgatcg ggccgcgtgc cgccccagac gtaaggcttg ccaagctgct
    13261 gctccaggcg gtgaatcgcc acttcggtga tgttatgcag gtgatcgctg cttaaaaagt
    13321 ggttgttttc cgccagcgcc tgccagcgtc tgtcactcgc cttgagcggc gcaggaaacc
    13381 attctggatg ctgttttatc tgctcccggt tgtggtggcg ctggcgagta aagggagcaa
    13441 aacgcataac gaaaacacgc ctggatgcaa aaatctgccg agcaagaacg aagacatgaa
    13501 agacataaac cggctgaatg aaggtgagaa ttttaaaaga ccggaggagt aaacgggatt
    13561 attaattgct tatttgcata ttactattga gatttcataa tattaggaaa taaatgatac
    13621 atgcgctttt cggactattg aaaactgccc ctgtgctaaa tttgtaaacc cagcgccgac
    13681 aggcttgcgt aaaactcttt tggtgataat gtgagccttc caaatccaga ctattccgat
    13741 ttgggatcca ttttgccgtc ctggcgtgtg taaccgtttt atcaaggaat aagcagtatg
    13801 cgtaaaatcg cattaattct tgcgatgttg ttgatcccgt gcgtttcgtt tgccggtttg
    13861 ctgggcagca gtagctcgac cacgccagtg agcaaagagt ataagcagca gttgatggga
    13921 tcccctgtct acatccagat cttcaaggaa gaacgtacgc tcgatcttta cgtcaaaatg
    13981 ggcgagcaat atcaactgct cgacagttat aaaatctgta aatattccgg cggcttaggg
    14041 ccaaaacagc gtcagggcga tttcaaaagc ccggaagggt tttatagcgt ccagcgtaat
    14101 cagttaaaac cagacagccg ttactacaaa gcgattaata ttggattccc caatgcttat
    14161 gaccgtgcgc atggttatga agggaaatac ctgatgatcc acggcgattg tgtatccatc
    14221 ggctgctacg caatgaccaa tcagggtatt gatgagatct tccagttcgt tactggtgcg
    14281 ctggtgtttg gtcagccgag cgtgcaggtg agtatttacc cattccgcat gaccgacgcc
    14341 aatatgaagc gccataaata ttccaacttt aaggacttct gggagcagct gaagccgggc
    14401 tacgactact ttgagcaaac ccgtaagcca ccgaccgttt ctgtggccaa tggccgttac
    14461 gtggtcagca agccgttaag ccacgaagtg gtgcagccac aactggcatc aaactacacg
    14521 ctccccgagg caaaataagt gccattcgcc tggcataatt ttttgccagg tttcattgcc
    14581 cgtcagcggc tgtgtcgcaa tcaccgtgac cacatcattc ggtgtggtct gcgagctgaa
    14641 gtcgatttcc acatcctgat ccagcaacgt tgccacgcca aacggcgcgc ggcgggtgat
    14701 ccagtgtaaa ttagtcgagc aatacgccat tacatagcgc ccgtccgaaa gcagcatgtt
    14761 gaaaacgccc ttcgcccgca gttcatccgc cagtgaggcg atatatttaa acactgccgc
    14821 catgttgccc ggcgtgcgcg ggtaacgctg cgttaattta tgcagcagcc agcaaaaggc
    14881 tttttcgctg tcggtttcac cgaccgggcg gaagttgccg gtttccagtg atttgtagcc
    14941 cgtcagttgt ccgttatggg cataagtcca gttacgcccc cataactcgc gggtaaatgg
    15001 gtgagtattt tccagcgcca cctcgccccg attagcctgg cgaatatgag ccaccaccga
    15061 acaggatttt atcgggtagt cctggacaag tttggcgatg ggggaattaa agctgggttg
    15121 tggatcttta aatgtgcgac agcctttacc ttcgtaaaag gtaatgcccc agccatcttt
    15181 atgtggcccg gttccaccac cacgctgtac aagtccggtg aaactaaagc agatatcggt
    15241 agggacgttg gcgctcatcc cgagcaattc gcacatgggt aacctccaca acagcgggag
    15301 cgcatggacc ccggcgaaga ttgatttaag aattttcagg tcggatgcgg cgtaaacgcc
    15361 ttatccggcc tacgccagac ttacttaacc atctcttttt caattaactg gatcaggata
    15421 tggatcactt taatgtgaat ctcctgaatg cggtcggcat aaccaaagtg cggaacgcga
    15481 atttcgatat ccgccgtgcc agccatttta ccgccgtctt taccggtcag ggtgatcact
    15541 ttcatcccct tctcacgcgc cgctgcgatc gctttgatca cgtttgcaga gttaccggag
    15601 gtggagatcc ccagcagtac atcgccttcg cgacctaccg cttcaacgta gcgggagaaa
    15661 atatcattga aaccgaaatc attaccgacg caggaaatat gactaacgtc agaaatagca
    15721 atcgccgggt agcccggacg gttttcacgg tagcgaccgg tcaactcttc ggcaaagtgc
    15781 atagcgtcgc aatgggaacc gccgttgccg caggaaagca ctttgccacc ggctttaaag
    15841 ctgtctgcta acaggaccgc cgcgcgctga atggcgtgaa tattggcgtc atcttttaaa
    15901 aagttagcca gcgtttccgc cgcttcgttc agttcgttac gaataagatc ctggtacatg
    15961 aggatatcct tcagcataaa tgtaatagac aaaatgcagt gtaccggata ccgccaaaag
    16021 cgagaagtac gggcaggtgc tatgaccagg actttttgac ctgaagtgcg gataaaaaca
    16081 gcaacaatgt gagctttgtt gtaattatat tgtaaacaca ttgctaaatg tttttacatc
    16141 cactacaacc atatcatcac aagtggtcag acctcctaca agtaaggggc ttttcgttat
    16201 gatgattttg agtattctcg ctacagttgt cctgctcggc gcgttgttct atcaccgcgt
    16261 gagcttattt atcagcagtc tgattttgct cgcctagaca gccgccctcg gcgttgctgg
    16321 tctgtggtcg gcgtgggtac tggtgcctct gaccattatc ctcgtgccat ttaactttgc
    16381 gcctatgcgt aagtcgatga tttccgcgcc ggtatttcgc ggtttccgta aggtgatgcc
    16441 gccgatgtcg cgcactgaga aagaagcgat tgatgcgggc accacctggt gggagggcga
    16501 cttgttccag ggcaagccgg actggaaaaa gctgcataac tatccgcagc cgcgcctgac
    16561 cgctgaagag caagcgtttc tcgacggccc ggtagaagaa gcctgccgga tggcgaatga
    16621 tttccagatt actcatgagc tggcggatct gccgccggag ttgtgggcgt accttaaaga
    16681 gcatcgcttc ttcgcgatga ttatcaaaaa agagtacggc ggactggagt tctcagctta
    16741 tgcccagtct cgtgtgctgc aaaaactctc tggcgtgagc gggatcctgg cgattaccgt
    16801 cggcgtgcca aactcattag gcccgggcga actgttgcaa cattacggca ctgacgagca
    16861 gaaagatcac tatctgccgc gtctggcgcg tggtcaggag atcccctgct ttgcactgac
    16921 cagcccggaa gcgggttccg atgcgggcgc gattccggac accgggattg tctgcatggg
    16981 cgaatggcag ggccagcagg tgctggggat gcgtctgacc tggaacaaac gctacattac
    17041 gctggcaccg attgcgaccg tgcttgggct ggcgtttaaa ctctccgacc cggaaaaatt
    17101 actcggcggt gcagaagatt taggcattac ctgtgcgctg atcccaacca ccacgccggg
    17161 cgtggaaatt ggtcgtcgcc acttcccgct gaacgtaccg ttccagaacg gaccgacgcg
    17221 cggtaaagat gtcttcgtgc cgatcgatta catcatcggc gggccgaaaa tggccgggca
    17281 aggctggcgg atgctggtgg agtgcctctc ggtaggccgc ggcatcaccc tgccttccaa
    17341 ctcaaccggc ggcgtgaaat cggtagcgct ggcaacgggc gcgtattctc acattcgccg
    17401 tcagttcaaa atctctatcg gtaagatgga agggattgaa gagccgctgg cgcgtattgc
    17461 cgggaacgct tatgtaatgg atgctgcggc atcgctgatt acctacggca ttatgctcgg
    17521 cgaaaaacct gccgtgctgt cggctatcgt taagtatcac tgtacccacc gcgggcagca
    17581 gtcgattatt gatgcgatgg atattaccgg cggtaaaggc attatgctcg ggcaaagcaa
    17641 cttcctggcg cgtgcttacc agggcgcacc gattgccatc accgttgaag gggctaacat
    17701 tctgacccgc agcatgatga tcttcggaca aggagcgatt cgttgccatc cgtacgtgct
    17761 ggaagagatg gaagcggcga agaacaatga cgtcaacgcg ttcgataaac tgttgttcaa
    17821 acatatcggt cacgtcggta gcaacaaagt tcgcagcttc tggctgggcc tgacgcgcgg
    17881 tttaaccagc agcacgccaa ccggcgatgc cactaaacgc tactatcagc acctgaaccg
    17941 cctgagcgcc aacctcgccc tgctttctga tgtctcgatg gcactgctgg gcggcagcct
    18001 gaaacgtcgt gagcgtatct cggcacgtct gggggatatt ttaagccagc tttacctcgc
    18061 ctctgccgtt ctgaagcgtt atgacgacga aggccgtaat gaagccgatc tgccgctggt
    18121 gcactggggc gtacaggatg cgctgtatca ggcagaacag gcaatggaca atttactgca
    18181 aaacttcccg aaccgcgtgg ttgccgggct gctgaatgtg gtgatcttcc cgaccggacg
    18241 tcattatctg gcaccgtctg acaagctgga tcataaagtg gcgaagattt tacaagtgcc
    18301 gaacgccact cgttcacgta ttggtcgcgg tcagtacctg acgccaagcg agcataaccc
    18361 ggttggttta ctggaagagg cgttggtgga tgtgattgcc gccgacccga ttcatcagcg
    18421 gatctgtaaa gagctgggta aaaatctgcc atttacccgt ctggatgaac tggcgcacaa
    18481 cgcgctgacg aaggggctga ttgataaaga tgaagccgct attctggtga aagcggaaga
    18541 aagccgtctg cgcagtatta acgttgatga ctttgatccg gaagagctgg cgacgaagcc
    18601 ggtaaagttg ccggagaaag tgcggaaagt tgaagccgcg taattgaata acggagccga
    18661 aaggctccgt ttctttatcc gctaattatt taaaattaaa gccatccgga tggttttcca
    18721 ggctgccggt caacgccgcg aacagcaccg ttttaccatc aatcgaaagc gcatcgttca
    18781 cattcagcca ggtgagtttc tcttgcgacg ttttctcatc aatagccgag aacagccccg
    18841 tcatctgatt agatttctcg gaccacatca cagcgatacg ttgcgagcca cagtcatgcg
    18901 gtttgcacgc gctcatcacc tgatacgtct catctcccaa cgttacggtt tgtgcgggag
    18961 tataagtacc gcctttcatc acccatgcag gtagcttatg cccttgcacc atctgattaa
    19021 acgcagcttt ggtggtttcg ccctttgcaa ggctgctaat ggttaaatca tcctgcgcca
    19081 ttgcactggt ggcgatgacc agtgcggcga ctgtcgttat tgccttaaac atcattcctc
    19141 ccgagcttat cctgcccatg ttattaacct cctaaagcct ggcgataaca cgataatttg
    19201 caaatcagat atttccaata tcagcatttg ttatcaattt attctgcgct aaggcaactg
    19261 cctgataagg tgttaaagtt ataaaaccct tcgcataaag gagttgtaca ccgtgcctag
    19321 tttgaagatt acgcttttgc agcaaccgtt ggtgtgggtg gatggtcctg ccaacctgcg
    19381 ccattttgat cgtcaactgg aaggtattac cgggcgcgat gtgatcgttc taccggagat
    19441 gtttaccagc ggctttgcca tggaagcggc agcttcgtcg cttgcacaag atgacgtagt
    19501 gaactggatg acagctaagg cgcagcagtg caatgcgctg attgcaggca gtgttgcatt
    19561 acaaacggag tctggttcgg ttaaccgctt tttgctggtt gagccgggcg gcacggtaca
    19621 tttttatgat aagcgtcatc tgttccgcat ggcagatgag catctgcatt ataaagcggg
    19681 caatgcgcga gtgattgtgg aatggcgcgg ctggcgtatt ttgccgctgg tgtgctacga
    19741 cttacgtttt ccagtgtggt cgcgcaatct caacgattat gacctcgccc tgtacgtcgc
    19801 caactggcca gctccgcgct ctctgcactg gcaggcattg ctgacggccc gcgcgattga
    19861 gaatcaggcg tatgtggcgg gatgcaatcg cgtcggcagc gatggcaacg gctgccatta
    19921 tcgcggtgac agccgggtga ttaatccgca aggcgagatt atcgctaccg ccgacgcgca
    19981 taaggcaacg cgtattgatg cggagctgtc gatggtggca ttgcgggaat atagagaaaa
    20041 gtttccggca tggcgggatg cggatgagtt taggttgtgg tgagtggggg ttgtgtttag
    20101 ggccgggggc tgggctagat tacgaaagcc cctaacttaa acacaatatt acgccagaag
    20161 tcaccatttc caaaacacag cgtcgctgta ccgtgggtta tctgcccaac cgcagcgaca
    20221 cgccggtgat caaaatcagc gccaaatggc tgagaaaagc gggatttgag atcggcacgg
    20281 gcgttaccgt gaagatctcg gagggatgtt tgatcctgct tgggataaca acgaagtaca
    20341 ggaactgcgc agggaacttt atcaggttaa acagccagtt aagggga
//
LOCUS       DAEEGA010000076        20217 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEEGA010000076 DAEEGA010000000
VERSION     DAEEGA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20217
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.076"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            12..278
                     /gene="mliC"
                     /locus_tag="KLH46_002921"
                     /pseudo
     CDS             12..278
                     /gene="mliC"
                     /locus_tag="KLH46_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
     gene            337..993
                     /gene="pdxH"
                     /locus_tag="KLH46_002922"
     CDS             337..993
                     /gene="pdxH"
                     /locus_tag="KLH46_002922"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBE0379526.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1122..2396
                     /gene="tyrS"
                     /locus_tag="KLH46_002923"
     CDS             1122..2396
                     /gene="tyrS"
                     /locus_tag="KLH46_002923"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBE0379527.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNRGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2455..3318
                     /gene="pdxY"
                     /locus_tag="KLH46_002924"
     CDS             2455..3318
                     /gene="pdxY"
                     /locus_tag="KLH46_002924"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBE0379528.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="KLH46_002925"
     CDS             complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="KLH46_002925"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBE0379529.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="KLH46_002926"
     CDS             complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="KLH46_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBE0379530.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6186..6821)
                     /gene="nth"
                     /locus_tag="KLH46_002927"
     CDS             complement(6186..6821)
                     /gene="nth"
                     /locus_tag="KLH46_002927"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBE0379531.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6821..7516)
                     /gene="rsxE"
                     /locus_tag="KLH46_002928"
     CDS             complement(6821..7516)
                     /gene="rsxE"
                     /locus_tag="KLH46_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBE0379532.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="KLH46_002929"
     CDS             complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="KLH46_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBE0379533.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="KLH46_002930"
     CDS             complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="KLH46_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBE0379534.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9203..11425)
                     /gene="rsxC"
                     /locus_tag="KLH46_002931"
     CDS             complement(9203..11425)
                     /gene="rsxC"
                     /locus_tag="KLH46_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBE0379535.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(11418..11996)
                     /gene="rsxB"
                     /locus_tag="KLH46_002932"
     CDS             complement(11418..11996)
                     /gene="rsxB"
                     /locus_tag="KLH46_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBE0379536.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11996..12577)
                     /gene="rsxA"
                     /locus_tag="KLH46_002933"
     CDS             complement(11996..12577)
                     /gene="rsxA"
                     /locus_tag="KLH46_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBE0379537.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12654..13094)
                     /locus_tag="KLH46_002934"
     CDS             complement(12654..13094)
                     /locus_tag="KLH46_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBE0379538.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(13180..13395)
                     /gene="ydgT"
                     /locus_tag="KLH46_002935"
     CDS             complement(13180..13395)
                     /gene="ydgT"
                     /locus_tag="KLH46_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBE0379539.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13668..13793)
                     /gene="blr"
                     /locus_tag="KLH46_002936"
     CDS             complement(13668..13793)
                     /gene="blr"
                     /locus_tag="KLH46_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBE0379540.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            14036..15076
                     /locus_tag="KLH46_002937"
     CDS             14036..15076
                     /locus_tag="KLH46_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBE0379541.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(15112..16113)
                     /gene="add"
                     /locus_tag="KLH46_002938"
     CDS             complement(15112..16113)
                     /gene="add"
                     /locus_tag="KLH46_002938"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBE0379542.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16217..17389)
                     /gene="malY"
                     /locus_tag="KLH46_002939"
     CDS             complement(16217..17389)
                     /gene="malY"
                     /locus_tag="KLH46_002939"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBE0379543.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17399..18991)
                     /gene="malX"
                     /locus_tag="KLH46_002940"
     CDS             complement(17399..18991)
                     /gene="malX"
                     /locus_tag="KLH46_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBE0379544.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            19166..20194
                     /gene="malI"
                     /locus_tag="KLH46_002941"
     CDS             19166..20194
                     /gene="malI"
                     /locus_tag="KLH46_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBE0379545.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT         5141 a         5119 c         5199 g         4758 t
ORIGIN      
        1 aaatataggg gcaaatccag gttgagcgaa tgcaaaccga tacgctggaa taccagtgtg
       61 atgaaaaacc gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg
      121 ataatcaact actgcatctc aaacagggcc tttcagcctc tggcgcgcgt tactccgacg
      181 ggatctatgt tttctggtcg aaaggtgagg aagcgactgt ctataaacgc gaccgcatcg
      241 tcttgaataa ctgtcagtta caaaatccac agcgttgaga tttttccagg ggcggcgcac
      301 aatagcgtca cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag
      361 caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt tacgccgccg cgatcttccc
      421 gccgatccat taaccctttt tgaacgttgg ctctctcagg cttgtgaagc caaactggcg
      481 gaccctaccg cgatggtggt cgctaccgtg gatgaacatg gtcagcctta tcagcgcatc
      541 gttttactca aacattacga cgaaaaaggc atggtgtttt acaccaacct cggtagccgt
      601 aaagcacatc aaattgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt
      661 gaacgccagg tgatggtgat cggtaaagca gaacgacttt cgactctcga agtgatgaaa
      721 tattttcata gccgcccgcg tgatagccag attggtgcat gggtttcgaa gcagtccagt
      781 cgcatttctg cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa
      841 cagggcgaag tgccattgcc gagcttttgg ggcggttttc gcgtcagcct tgaacagatt
      901 gagttctggc agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgtgaaaat
      961 gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg
     1021 ctggtactcc tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt
     1081 gtaccggcaa aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga
     1141 ttaaacaatt gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag
     1201 agcgactggc gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct
     1261 tgcatttggg gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca
     1321 agccggttgc gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg
     1381 ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc
     1441 aggttgcccc gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact
     1501 atgactggtt cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct
     1561 ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg
     1621 ggatttcgtt cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga
     1681 acaaacagta cgatgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt
     1741 ctggtatcga cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc
     1801 tgatcaccaa agcagacggc accaaatttg gtaaaactga aggcggcgca gtctggttgg
     1861 atccgaagaa aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg
     1921 acgtttaccg cttcctgaag ttcttcacct ttatgagcat tgaagagatc aacgccctag
     1981 aagaagaaga taaaaacaga ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg
     2041 tgactcgtct ggttcacggt gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc
     2101 tgttcagcgg ttctttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg
     2161 gcgtaccgat ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg
     2221 aactgcaacc ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta
     2281 acggtgaaaa acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc
     2341 gttttacctt actgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca
     2401 ttaagtggaa agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg
     2461 aaaaatattc tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca
     2521 gagtttccga tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct
     2581 aatcacaccc aatacggcaa atggactggc tgcgtgatgc cgcccagcca tttaaccgaa
     2641 attgtgcaag gcattgccgc cattgataaa ttacacacct gtgatgccgt attaagtggc
     2701 tatctgggat cggcggagca gggtgaacat atcctcggta tcgtccgtca ggtgaaagca
     2761 gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt
     2821 atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc
     2881 attgcgccga atctggttga gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa
     2941 gaagcggttc tggcagcgcg cgaactcatt gagcaagggc cacaaattgt gttggttaaa
     3001 cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctggt caccgccgat
     3061 gaagtctggc atatcagccg tccactggtg gattttggta tgcgccagcc ggtaggtgtt
     3121 ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag
     3181 gccctggaac atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag
     3241 gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga acattacttc
     3301 agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg gccgatggct
     3361 attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt
     3421 gcataaatgc cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc
     3481 gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct
     3541 catccttcag tgcctcgttc acatattgca gcttcttatc cagctgcgcg cgaactgtcg
     3601 gtttgtactc ttccggtgta tctgggcgaa acagaggtgt gaaaccttta tgcagctcgg
     3661 tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca
     3721 gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgcaacg ccttccgtca
     3781 gcaaagtacc atcgtccagc agcaatgcag gcacctgccc cttagggtta acggaaaagt
     3841 aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagc gtaaaatcct
     3901 ttccgctctc acgcagggtg atatgggaag cgagagagca ggcgcccggt ttgtagaaca
     3961 atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa
     4021 aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta
     4081 cagtagcttt cgccgctttg tctgcagcgt catcctgcgt catgcggtgc agtttaggcg
     4141 cggtcagcag catcagtact gcaataacgg cagtagcgac accaatctgc aagaatactc
     4201 gaccatagac ttccagtgac atcagcgggt cggtaacgtt atccggcaca gccatcatac
     4261 ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa
     4321 tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga
     4381 tcatcagttc cccgatgctc tgcaggccat agcttgcgac cagccagctg acagacacga
     4441 tacctgcgtc agacgcgaat ttcgcaccca gcggcagaat caggaacgca ccagaacaca
     4501 tcaccatgcc gattgcaaac ttggttggca tcggcagggt atcacccatc ttgttataga
     4561 tagcggccag aatcggacta ccgatgatga tccagaacgg gttcagtgcc tgatactgct
     4621 caggttctac ggccagaccc agaatggagt gctcaacgtt acgaatcgca aagaagttca
     4681 gtgacgttgg catctggctg tacagcacga agaagatgat tgcttcgagc atcaggatga
     4741 aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaagcttct ttaccgaaga
     4801 taaccacaat accgaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca
     4861 gtagccaggt ggcaacagcg atcagcgcca caacaccaat aatggtcagc aacaggttac
     4921 ggtagttgac tggttcaaag tctggttttg aaccgtactg tttaacccag cgttggcaga
     4981 aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caacgcaaac gcaacactcc
     5041 agccgtattt cgcggccagc cacggggtgg ctatcataga gaagaaagag ccgatgttga
     5101 cggacatgta gtacatggtg aatgcaccgt ccagacgtgg gtcgtttttc tcatagcatg
     5161 tagaaagcag agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca
     5221 tacccatata aacaataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg
     5281 ccagcacaat agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc
     5341 agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg
     5401 aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc
     5461 cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt
     5521 gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta
     5581 cctctttttt tacatcccat attaacgggg gtgctttctc gcgttatgtc taaaaaacat
     5641 acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc
     5701 agacaagtcc tttgtaataa tctgttacat ataactgagg cggggaattc aaccattccg
     5761 ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac ggcagatttt ttcactgcat
     5821 tgagaggggg atgatggctt ggcggcgata tgttctgcta tttagcgttt ttcctagtga
     5881 gatagtccag tttctgaaaa atagccagtg taatgttttg caggtcaata tttgttgcgt
     5941 gtgtaaattt aaatcatttt aacctgattc ttgcaatgtt tttacattgc cacagcatcg
     6001 tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta acctgcattt
     6061 ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc attgacgtta
     6121 aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg tgttacccct
     6181 tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat acaagagcca
     6241 cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca atggtggcag
     6301 tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg ttcgacgttt
     6361 ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata
     6421 gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc tacgccgggc
     6481 agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgttgttc cagtaatata
     6541 cggcaggttt tgatgatatt ttctgctttg ctgttataaa ggccaatcgt tttgatatag
     6601 gttttcaccc cttcaacgcc tagttcaagc atcgctgcag gcgtattcgc caccgggtag
     6661 agtttcgccg tcgccttatt aacactgata tcggtcgcct gagcggaaag cagtacggca
     6721 atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg attgttctca
     6781 cgcaggcgag tgaggatctc cagacgtttt gctttgttca tcagacattc cctgtttcac
     6841 cgtttggcaa tgcacgttcg gcagctgctt cagcacggcg ctttttcatt ctttcatcaa
     6901 tcaggtattt tcctgccagc atcagtccca agccaataaa tgcacctggt ggcagcatcg
     6961 ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt gcccagctac
     7021 ctaacagcgc atctgcaccg tcaaacaacg tgccattgcc gataatttcg cgtagtgaac
     7081 ccagcacgaa catggcgcag gttgcgcccg taccaattga aaagccgtcc agtgccgaaa
     7141 gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata cagttagtga
     7201 caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag gcgttgatca
     7261 gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga atgcggatct
     7321 cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg
     7381 tagtcgcaag tcctaaaccc agagcgttag tggcagtgga tgtgaccgcc aatagcggac
     7441 aaaggccgag cagttggacc agtgcagagt tgtttttcca caacccctga acaataacgt
     7501 ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt tgtgccggta
     7561 acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg
     7621 tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag tgcgcatcat
     7681 ccgcaccact gatttttttt cccgcaaaat gggtgagcca gtcagaaagg cgcaattcga
     7741 ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc
     7801 cattaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct ggcgcggttg
     7861 cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac
     7921 ctttacctaa ctctggcgca gttaccagat agcaactctg tgcgagcgca ttgttatagc
     7981 gttcggctgg cagcacctga tcaaataacg tcttttgttg cagactggcc tgttcagcaa
     8041 ttgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgtagaaccc gctgcaaata
     8101 gtgccagtgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct ttgcgatggc
     8161 cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc gccagcagga
     8221 cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag actaataagc
     8281 ccgccagcgc gccgaacatc agccgaccac gattggtcgt agaagcagta actgggtcag
     8341 tcaaaataaa gaatgcgccg agcatagtcg caccagacag cagatgaatt tgcggtgctg
     8401 ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc gttactaaga
     8461 agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacacg ccaccagcca
     8521 gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg tagatcggat
     8581 attgcataat ctgttcaacc gaatgacctg cgcggacaga ggttttgaat gtatccagcg
     8641 gtgtcgcctg actaatgcca tcaataccta agcgtagcgt attcatatca ccaccactgg
     8701 cggtatgacc gctgaaaata acctgtatgg cgtcgataaa accagggatg ttgaccgcaa
     8761 tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt aagaccacat
     8821 aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac tgtttagcga
     8881 tgatcaccgc aaacaccgta cccagtacga ccatccacca tggcgctagg gggggaatac
     8941 ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg
     9001 actgcttgcg tagtttgagt acgagagctt cggctaacag agcactgacc gacgccaaca
     9061 ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt cctggcacgg
     9121 ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg gtataagggg
     9181 agctagctat tctgaatacc atttagtcct cgtttacaac cttttgctgg gcggcttttt
     9241 tggcctgaac gcgggcaata gccgcggcaa ctgcggcttt gcgcggatcg acctgttctt
     9301 ctggttccgc attagcctgt tgctgttcca gcttgcgtgc tttggcgcgg gcaatagcgg
     9361 cttcgacggc agctttgcgc ggatcaacct gttcttctgg ctccgcattg gcctgttgct
     9421 gttccagctt gcgtgctttg gcgcgggcaa tagccgcttc gacggcagct ttgcgcggat
     9481 caacctgttc ttctggctcc gcattggcct gttgctgttc cagcttgcgt gctttggcgc
     9541 gggcaatagc ggcttcgacg gcggctttgc gcggatcgac ctgttcttct ggttccgcat
     9601 tagcctgttg ctgttccagc ttgcgcgctt tggcacgggc aatagcggct tcgacggcgg
     9661 ctttgcgcgg atcgacctgt tcttctggtt ccgcattagc ctgttgctgt tccagcttgc
     9721 gcgctttgga gcgggcgata gctgcttcaa cggcagtttt acgtggttca gcaacggttg
     9781 ctgcgtcgtt agtttgctcc agttctgcct gtttcgctct ggcttgcgct ttacgggctt
     9841 cccgttctgc aataattgca ctgttatccg ggcgttcgcc cgctttaatc acaataggct
     9901 gtgtagcctg ggcctgtttc tctttcaccc gcgccagagc ggcagcaatc gcatctttat
     9961 ctttggctgc aggttgaacg gctgcgctct tatgtcgttc aaggcgagtc gctttttcgc
    10021 gctccagacg agcctggcgc gcttcgaaac gcgctttggc ttctgcggcg cgcttttctt
    10081 cctgacgaat agccgcaatt tcagcttttt cctgacggaa atattgcacc aagggaatat
    10141 tgctcgggca aacccacgcg caagccccac attcaatgca gtcggcaatg ttatgcgtgg
    10201 tagctttatc gtgttgctga cctttgctga accagtacaa ctgttgcggc aaaagatcca
    10261 cagggcaggc gtcagcacag gcgctacacc ggatgcagct ttgttcttcc tgtggttcgc
    10321 caagttcatt ggcagaggga gccaacagac agttggtaat ctttacgacc gggacatcca
    10381 gccatggcaa ggtaaagccc attagcgggc cacccataat caccatttga tcggcagagg
    10441 ggcagaatcc ggcatcattc aataaatgac gcactggcgt ccccagccgt gcccagacgt
    10501 tgcccgggcg agcgattgct tcgccagtca gggttacaac acgctcggta atcggctcgc
    10561 catcaataac ggcacgtttc actgcataag cagtgccgac gttttgcatt aatacgccga
    10621 tatcggatga acgcccgcca tgtggaacct gcttcccggt cagaatgtag gttaattgtt
    10681 tagcaccgcc agaaggatat ttggttggaa tcacccgcag agaaatatcg ttagagtccg
    10741 ccagcaccgc gcgcagcatg gaaatcgcct gcggtttgtt atcttcaatg ccgataagaa
    10801 tttcgcgtgg ctgcagaata tgcgcaagaa tgcgaatacc ctctacgacc tgagccgcgc
    10861 aatcctgcat caaacggtca tcggcggtaa tgtacggctc gcactcagca gcgttgataa
    10921 tcaacgtttc aatcttatct ccaccaccct gcaatttaac gcctgtcggg aatccggcac
    10981 cgcccagccc ggcaacacca aactgatgta tgcgctcgat taactcttcg cgactgcgag
    11041 agcgataatc gacccagccg tcgcgcggga tccagcagtc ttcaccatcg gcatcaataa
    11101 tcacgcttaa ttcagctaaa gctgaaggat gagccgtaga gtggggcgca atagccgtaa
    11161 cggtacccga ggtgggcgcg tgaacaggca gcattttgcc gcgaccacgg gtaagcggct
    11221 ggccgcgcaa tactttatcg ccgacgctaa cgcacaactc accttcagcg ccaatatgct
    11281 gtttcagtgg aataacaaaa tgctgcgcca gtagtacctg gcgcaggggg gtaccgttgg
    11341 tctgggtttt catctccggt ggatggatgc cgccgttgaa atcccagatt ttattttttc
    11401 tgaatgcaga gaataactta agcatggtgt tccacgggaa tgatacgcac gggaatggtg
    11461 ttcagatccc atttccagga gtcaggtgtt tctgcgaccg gttgcaacga gatgcagtgc
    11521 gtcgggcacg gatcaacaca taaattgcag cccgtacaga gatcactcat taccgtatgc
    11581 atgacacggg tagcgccaac gatggcgtct accggacaag cctgaataca tttagtgcag
    11641 ccaatacagt tattttcatc aataaccgcc accatccgcg caggcgttag ctcttgcgct
    11701 tcgccatcca gcggctgcgg ctcgacatta agcaactcgg caatttttag catcacagct
    11761 tcgccacctg gggcgcaacg gttgattttt tcaccgttac agctgatggc ttccgcgtag
    11821 gggcgacagc cgggataacc gcactgacca cactggctct gcggtaagat ttcgtcaatt
    11881 ttctcaacga ccggatcgtc ttccaccgca aaacggcggg aggcataacc cagaatggcg
    11941 ccaaacgcca ggcccagcag gctcacggcg gcaacggcaa tccagatagc attcattaca
    12001 acttcaccaa accactaaag cccataaagg ccagagacat aagacctgcg gtaattaacg
    12061 caatggcatt accgcgaaaa ggtgccggga catcagccac agcaaggcgt tcgcggatgg
    12121 cggcgaagag caccatcacc agcgagaaac cgacagcggc ggaaaaaccg tacagcgccg
    12181 actgcaagaa attgtgcccg agattgatat tcagcaacgc cacgccgagc actgcacagt
    12241 tggtggtgat aagcggcaaa aaaatcccca gcagacggta aagcaccggg ctggttttgc
    12301 gcaccaccat ctcggtgaac tgcacgacca cagcaatcac cagaataaat gccagggtgc
    12361 gcaggtaaat cagattaagt gggatcaaaa tccacgtatc gataagccag gcgcaaatag
    12421 acgtcagcgt catcacaaac gttgttgcca gccccatgcc catcgcggtt tccagttttt
    12481 tggaaacccc cataaacgga cagagaccga gaaacttgac cagtacaaag ttattgacca
    12541 gtacagttcc gacaaacagt aacaggtagt cagtcatttt tcggcctgaa ataaaaaagc
    12601 cgcctattat ccgttaatcc agagcaggcg acaacaggtt aactgtaagg ttattacgga
    12661 ttcacaaacg tcgcttttac ccgcgacgag cgtttgaaat aggggacgat caatgcggtt
    12721 gccaacaaag taaacagcaa ctggcgaacg gctatgccaa cttccacggg tgaaaaagcg
    12781 aatgctttta ctgccagcaa tacggaaata agcagccaga taatatagtg tttaggaaca
    12841 caacgccgac gtttaaagaa agcgatggtc aaccacagtg tgtaatacca catggcgata
    12901 gcggtaataa aagaaacgcc ccataagatt tgtgtcgtca gtgcttgtcc gccgagcgtt
    12961 tgaaatgttt gcggagaaga taacgcagcg gtatacagca acaacgccag cgtcgtactc
    13021 aacagagcga ctaaaagcca ggccaacggg ccaagcaacc agcctccaat acgttgtggc
    13081 gttgtggtgg tcataggttc tccagaatag cgacgcaaac aggcaaatct aattttaggg
    13141 gcgttattat aaggctttta taaccgatcg ctactttctt tattggacat agtgccagac
    13201 ggacttcggt acctggccga ggtcaaacaa acgcccgcca gaaaccagct ctgcgcgacg
    13261 gtgatcggca gcacgataca tattgatcag ttcctgatcg tccgtcaggg tgtaattaag
    13321 atggtcgtag agtttttcca gactttcgag tgaactgatt ttgcgaaatt ttaataagta
    13381 gtcctgaaca gtcataaaag gtccataaaa taattaatac caaaggggta ggtattatta
    13441 gtgatgataa aatttcacca aaagtgaacg cttgtgttat tacaataata agaatgcgct
    13501 gatggcaaat attcagggat gaaaaactgc tctgaaaaga gtaacgcgct ttactatccc
    13561 tgacaatcac caacaacatc gaacaagata ataaattcct ggtttaatat ccgacaagtg
    13621 aaaacatgca cccggacggg cagcatgtcg ctccacaagt gcagagctta cttgtgttgt
    13681 accgaagcac tctgttcagg tggctgatag ttgtcaatgt gactcgccac gccaagaaga
    13741 atgactgaaa cgacaagaac gatccaacct gttaattcaa taagacgatt cattacagcc
    13801 cacatctctc ttgattgatc cattaacttc agggggtaaa tgttacttag caataatagc
    13861 tccagcaaga tttttactga ggtttttgcg atatttagct tttgtcgttg gaaaattcgc
    13921 tatttttttg acttaagtta aacaacatcc cttattgctg gcaggttatt aaactgttga
    13981 gcgtgggtaa aggatagtgt caaatagcca tcatacttca atgagaggca atgacatgag
    14041 cgacaacatc cgtgttgggt tgattgggta tggttatgcg agcaaaacct tccatgcgcc
    14101 cctgattgcg ggcacgcccg ggctggaact ggcggtaatc tccagcagtg atgaaacaaa
    14161 agtaaaagcc gactggccaa cggttacggt tgtctctgag ccgaagcatc tgtttaacga
    14221 tcccaacata gacctgattg tcattcctac acccaacgat acccatttcc cgttagccaa
    14281 agcggcgctt gaggcgggta aatatgtggt cgttgataaa ccctttaccg tgacactgtc
    14341 acaagcgcga gagctggatg cgctggcaaa aagcctgggg cgtgtgctgt ctgtattcca
    14401 taaccgtcgt tgggatagcg atttcttgac gctaaaaggt ttgctcgcgg aaggcgtgct
    14461 gggtgaagtt gcttactttg agtctcattt tgaccgcttc cgtccgcagg tgcgcgatcg
    14521 ttggcgtgaa cagggcggcc caggcagcgg tatctggtac gatttagcac cgcatcttct
    14581 tgatcaggcc attacgctat ttggtttacc ggtcagcatg acggtagatt tggcacagtt
    14641 acggcccgga gcgcagtcga ccgattattt ccacgccatc ttgtcctatc cacagcggcg
    14701 agtcatttta cacggtacca tgctggcagc cgctgagtca gcacggtata tcgtgcatgg
    14761 atcccgaggc agttatgtga aatatggcct cgatccacag gaagaacgtc tgaaaaatgg
    14821 cgagcgtctg ccgcaggaag actggggcta cgatatgcgt gatggcgtac ttacccgcgt
    14881 ggaaggtgag gaacgtgtcg aagaaacgct gttgacggtg cctgggaatt atccggctta
    14941 ctatgcggcg attcgtgatg cgttaaatgg cgatggtgaa aatccggttc cggcaagcca
    15001 ggcaatccag gtaatggagt tgattgagct gggcatcgaa tccgccaaac atcgcgcgac
    15061 gttgtgcctt gcgtgaaaaa tggatggtgc aatttgcacc atccattttt gttacttcgc
    15121 ggcgactttt tctcgcagtg cgcgtttttc ctctgcgctg aggaaagcca tttccagacc
    15181 attaatctgt gcctggcgga tttgctcacg ggataaccca gcagctggcg cggcaacggt
    15241 atattcgtga atgatatcca ctccctgtac gccgggatcg tcagtgttaa tgctggcacg
    15301 aatgccatgc tcaaggaacg ttttcagcgg atgtgcagcc agctctgcta cggtgctggt
    15361 ctgaatattg gaggtcagac aggattcaat accaatttgt tgctcggcga gaaaatccat
    15421 cagcgcccga tcttcaatgg cttttacgcc atgtccaata cgctccgcac ccagttcacg
    15481 aatcgcctgc cagatgcttt ccggcccggc agcttcgcct gcatggacgg taatatgcca
    15541 gcccgcatca cgcgcgcggt tgaagtgaga aaggaacaga cttcccggga atccaagttc
    15601 atcaccagct aaatcaagtg cggtaatctg gtcacggtgg gctaaaaagg cctccagctc
    15661 ttgctgacag gcggcttcgc cgaaggttcg gctcataatg ccgataagct tcgcctgcac
    15721 accaaaggtg cggcaacctt cacgtacgcc atcgatcacc gcttcgacaa cacccgctac
    15781 aggcagctta tgtgccattg ccatgtagcc tggtgaaaaa cgcagctcga catagcgcag
    15841 gccgttacgg gctgcatctt caatgttttc aaatgccacg cggcggcagg catcaagaga
    15901 ggcgagaact ttaacgcccc agtcaagttt ggtcagaaag ctcaccagat cgggttcgtt
    15961 ggcaatgacc tgaacgtggg gaatcagtgt ttcaagggat tgtgcaggaa gcgagatatt
    16021 atactggcgg ccaagttcaa gaatggtctg gggacgaatg ttgccatcaa ggtggcgatg
    16081 gatatcagtt aatggcaggg tggtatcaat catggtcgca ctctttttct ggatataaaa
    16141 gtgcgcacca ttataaaaac aaagcagggt aaaaatccac gattgcgcaa cgatttttta
    16201 cgttgcgcaa ttggggttag cgaacagcgc ggatggcgtt tattaatcca gccacacctt
    16261 tttccagttt cgaacgtggg cagccggcat tgagacggac aaaaccacga ccttcttcac
    16321 cataggtata ccccggcatg atcgcgactt tttcttgctc gataagcgct ttttgcaacg
    16381 cgttgtcgtc aatattcaac ggacgtagat caagccaggc cagataagtg gattgcggga
    16441 tctgccagtt gagttcagga aacgcggcgt tcattttatc tgagatatac gtcaggttat
    16501 ctttcagata gacgcgtaag gcatccagcc acggtgcacc ttgctgatag gcggcgatat
    16561 gggcagttaa cgccagtacc gaaggggaag aaagcccatc acgtcctttc agtgccgata
    16621 aataggcatc acggctgctg ctattttcta taatcccgta agcaccggtc agcgcgggaa
    16681 tattgaaact tttcgatccc gatgtcagca acgcccagtc tccccgcgcc acattactcc
    16741 agggaatatg cggctgctcg ccccagacca tatccatatg gatttcatcg gaaataaccc
    16801 gcacatcatg acgctcgcac aggtcagcca tgatctccag ctcatcgcac gtccacactt
    16861 tcccggtagg attctgtggg ttacacagga gcataatttt acattctggt ttcgccaaaa
    16921 cggcttccag cttgcccata tcgcaaaacc agccgtcagc ctgcttctct aaagcaacgg
    16981 gcattactgt gcgctggtta ccttcaatgg ccttgtaaaa tgcgtcatag gcgggcgtgt
    17041 ggatcaccac gccttcacct gtttcagacc actgacgaat cagttctgaa accatataga
    17101 tgacagaagg gccatacacc accgtctgcg tatcaattgc ggtgtaatgc tgggtggaaa
    17161 accagtgggc aatagccgcg agaaactcat cgtttttcca gcggctgtag ccaaatacgc
    17221 cgtgcatcag gcgctgattc agcgcctcga taatgcaggg ggcagtggca aaatccatgt
    17281 ctgaaatcgt gaacggtaac aggtcagcag tgccgaaacg gtcagcgaca taatcccact
    17341 gtgtacacca tgtgccatga cgatccacga cctttgaaaa atcgaacata tcttatcctt
    17401 atgcctggac agtatgcatc agaccggcca tttcatcttt tactgactga acttgtggcc
    17461 cgataacaac ctgcaggtta tgttgattaa gttgtactac gccaattgcc cgattgtcct
    17521 tcagtgcctg cacattaaca agcgacatat cttccacaga caaacgcaga cgggtaatgc
    17581 agttatcgag gctgacaata ttgtcggcac cgcctaatgc ttcgaggatt gcaggaacgt
    17641 tgtaacctga tttacccggc gcaccggcaa cggctttttc gattgagctg gcaacttcgc
    17701 tatcgcgccc cggggttttc agattgaagc gggtgatagc gaaacggaag atgacgtagt
    17761 aaacgacaaa ccagattgcc gccaccactg gcaccatgta ccacttggtt gacagaccat
    17821 gcaaaatacc gaacaccacg aagtcgatga tattgccgtc ggtattaccg atggtgacgc
    17881 cgagcacaga catgacggtg aagccgaggc cggttaacag cgcgtggatg acatacagaa
    17941 ctggcgctac gaacaggaac aggaattcca gcggttcggt agtgccgcca acgacgcagg
    18001 cgatcaggcc agaaatcagc agacctttaa ttttatggcg attttccggg cgcgcgcagt
    18061 gatacatagc taacgctgca cctggtagac cgccgagaaa cgcaggcatt ttaccttgcg
    18121 aaaggaaacg cgtgacgctt tcagaaaaac cgtgagtggt cgggcaactc aattgcgcct
    18181 ggaagatggt cagtgcgccg ctgacggttt gaccgcagac ctcctgcgtg ccgcctgcgt
    18241 cggtaaagcg aattaatgcc accagaatgt gatgcagacc aaacggcaac agcagacgtt
    18301 caccggtacc aaacagcatc ggtccgaaat cacccgcgct gtttatcata tggcccaagc
    18361 cgctaatacc catggcgaaa atcggccaga ctaatggaat caccaggccg acaaggccca
    18421 tcaccagcga ggagataatt ggtacgaagc gcgtaccgcc gaagaatgcc agcgcatccg
    18481 gcaggcggat attatggaaa cgctcatgca gcatccagac gataataccg gcgatcaccg
    18541 caccgaggat cccggtatcg atcgactgga tcccaagaat gctctggatg ttattggctt
    18601 tcagaacggc ggcatctgtg gttggcagaa tgcctttatt ggtcaaccag aagtttaccg
    18661 cgaggttcat taccgcataa ccgacgaagc cagcgaatgc cgctacgcct ttattttcgc
    18721 gtgccaggcc cagcgggatg gcgatacaga acatgacagg caggaaacta aaagcaaacg
    18781 agccaatctt actcatccag gtaaagatag cctgcaacac ggggttgccc aggaccggga
    18841 tcagggttat gacatcatgg ctgctaagag aactaccaat gccgagcata atgccgcaga
    18901 acgacaataa tgccacgggt aacatgaagg ttttgcctaa ctgctggaag aactcccaca
    18961 gcgtgacttt cggtgctgtt ttcgccgtca taaaacgact cctcgtagag aacaatctgg
    19021 attcagtaaa ttgcgcgaga agataaaacg ttttatcaaa ttttagtgag tcataaatca
    19081 cattacgcaa cgataatagc gggtataaga taaataaaag gtaaaacgtt ttatctgtca
    19141 cataatcagg gagtaggtca tctgcatggc taccgccaaa aaaataacca ttcatgatgt
    19201 tgcgctggct gcgggcgtgt cggtaagtac cgtttcgctg gtgcttaatg gcaaagggcg
    19261 aatctctacc gccacaggag aacgcgttaa cgccgccatt gaagagctgg gatttgtgcg
    19321 caatcgacag gcgtcggcgc tgcgcggcgg gcaaagcggc gtcattggtt tgatcgtccg
    19381 tgatttatct gcgccgtttt acgccgaatt gacggccgga ttgacggaag ctctggaagc
    19441 acagggacgg atggtttttt tgcttcacgg cggtaaagac ggcgagcagc tggcacagcg
    19501 gttttcactg ttactgaatc agggtgtcga tggtgtggta attgccgggg ctgcaggaag
    19561 cagcgatgac ctgcggcgga tggcagaaga aaaagctatc ccggtgattt tcgcttcccg
    19621 tgccagttat cttgatgatg ttgatacggt tcgcccggac aacatgcagg ctgcacagtt
    19681 gttgacggag catctcattc gcaatgggca tcagcggatc gcctggctgg gagggcaaag
    19741 ttcctcatta acccgtgcag aacgggtggg gggctattgt gcaactctac taaaatttgg
    19801 cctgccgttt cacagcgatt gggtgttgga gtgcacttcc agccagaagc aagccgcgga
    19861 agctatcacg gcgcttttac gtcataaccc gaccatcagt gccgtggttt gctataacga
    19921 aactattgcg atgggggcat ggtttggttt gctgaaagca gggcggcaaa gcggggaaag
    19981 cggagtcgat cgttactttg agcaacaggt ttcgctggcg gcatttaccg atgcgacacc
    20041 aaccacactt gatgatatac ctgttacctg ggccagcacg ccagcgcggg aacttggtat
    20101 cacacttgcg gatcgcatga tgcaaaaaat cacccatgaa gagacgcatt cacgcaatct
    20161 tattattccc gcccggctca ttgcggcgaa ataatcctct ctttatctgc tatactt
//
LOCUS       DAEEGA010000077        19414 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEEGA010000077 DAEEGA010000000
VERSION     DAEEGA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19414
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.077"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            111..2201
                     /gene="pbpC"
                     /locus_tag="KLH46_002942"
                     /pseudo
     CDS             111..2201
                     /gene="pbpC"
                     /locus_tag="KLH46_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2351..2782
                     /gene="ndk"
                     /locus_tag="KLH46_002943"
     CDS             2351..2782
                     /gene="ndk"
                     /locus_tag="KLH46_002943"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBE0379546.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="KLH46_002944"
     CDS             2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="KLH46_002944"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBE0379547.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4371..5384
                     /gene="rodZ"
                     /locus_tag="KLH46_002945"
     CDS             4371..5384
                     /gene="rodZ"
                     /locus_tag="KLH46_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBE0379548.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGVWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5411..6538
                     /gene="ispG"
                     /locus_tag="KLH46_002946"
                     /gene_synonym="gcpE"
     CDS             5411..6538
                     /gene="ispG"
                     /locus_tag="KLH46_002946"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBE0379549.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6641..7915
                     /gene="hisS"
                     /locus_tag="KLH46_002947"
     CDS             6641..7915
                     /gene="hisS"
                     /locus_tag="KLH46_002947"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBE0379550.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7933..8553
                     /gene="yfgM"
                     /locus_tag="KLH46_002948"
     CDS             7933..8553
                     /gene="yfgM"
                     /locus_tag="KLH46_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBE0379551.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8564..9742
                     /gene="bamB"
                     /locus_tag="KLH46_002949"
     CDS             8564..9742
                     /gene="bamB"
                     /locus_tag="KLH46_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBE0379552.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9860..11332
                     /gene="der"
                     /locus_tag="KLH46_002950"
     CDS             9860..11332
                     /gene="der"
                     /locus_tag="KLH46_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBE0379553.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11402..11617
                     /locus_tag="KLH46_002951"
     CDS             11402..11617
                     /locus_tag="KLH46_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBE0379554.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="KLH46_002952"
     CDS             complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="KLH46_002952"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBE0379555.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13146..14612
                     /gene="guaB"
                     /locus_tag="KLH46_002953"
     CDS             13146..14612
                     /gene="guaB"
                     /locus_tag="KLH46_002953"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBE0379556.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14681..16258
                     /gene="guaA"
                     /locus_tag="KLH46_002954"
     CDS             14681..16258
                     /gene="guaA"
                     /locus_tag="KLH46_002954"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBE0379557.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16353..16892)
                     /locus_tag="KLH46_002955"
     CDS             complement(16353..16892)
                     /locus_tag="KLH46_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBE0379558.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16908..17426)
                     /locus_tag="KLH46_002956"
     CDS             complement(16908..17426)
                     /locus_tag="KLH46_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBE0379559.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17737..17928)
                     /locus_tag="KLH46_002957"
     CDS             complement(17737..17928)
                     /locus_tag="KLH46_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBE0379560.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17946..18098
                     /locus_tag="KLH46_002958"
     CDS             17946..18098
                     /locus_tag="KLH46_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379561.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18280..>19414
                     /gene="pdeF"
                     /locus_tag="KLH46_002959"
     CDS             18280..>19414
                     /gene="pdeF"
                     /locus_tag="KLH46_002959"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBE0379562.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4568 a         4729 c         5278 g         4839 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacaggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca cgttaccttg
      121 aagcgctgat caattatgaa gatcgctggt tctggaagca tccgggggtt aatccattct
      181 cggtggcgcg cgcagcatgg caagatctca cttcgggacg ggttatttcc ggtggcagca
      241 cgctcactat gcaggttgct cgtctgcttg atcctcaccc caaaacattt ggtggcaaaa
      301 ttctccagct ctggcgcgcg ttgcaactgg agtggcattt gtctaagcgt gaaattctga
      361 tcttgtatct taaccgcgct ccgtttggcg gtacgttgca ggggatcggt gcggcaagtt
      421 gggcttatct cggaaaatcg cctgcgaatt taagctattc cgaggcggca atgctggcgg
      481 tgttaccgca agtgcccagc cgactgcgcc cggatcgttg gccggagcgt gccgaagccg
      541 cgcgtaataa agtgctcgaa cggatggctg cgcaaggtgt gtggtcccgt gagcaggtaa
      601 aagagtcaag ggaagaaccc atctggctgg caccccgaca aatgccgcaa ctggcaccgc
      661 tgttttcgcg catgatgctc ggtaaaagca aaagcgacaa aatcgttact acgctggatg
      721 ccggtcttca acgacgtctg gaagaactgg cgcaaaactg gaaagggcgg ttgccaccgc
      781 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      841 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      901 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      961 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
     1021 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga
     1081 acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac
     1141 gcaatgttgg attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc
     1201 tcggcggcgc tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc
     1261 acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa
     1321 tgtcgtcggg ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc
     1381 cggatagtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct
     1441 atcgtgacgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca
     1501 gacctgacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga
     1561 atcaggtcaa taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga
     1621 actcagtcac tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg
     1681 gtaactgtcg ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt
     1741 tactgtcaga gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag
     1801 ggcagcgcgt cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc
     1861 taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct
     1921 cgacaatttg cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc
     1981 gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat
     2041 caagtggagg ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg
     2101 ggcgcaacgt gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg
     2161 atgtggggca aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg
     2221 ttgctaaaac tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg
     2281 ttgctcatta taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac
     2341 agaggtaaaa atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa
     2401 aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac
     2461 caaaatgctg catctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa
     2521 accgttcttt gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct
     2581 ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa
     2641 cgcactggct ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca
     2701 cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga
     2761 agtgtgcccg cgcacctgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg
     2821 cgccagaatt tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt
     2881 ttttcaaccc tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa
     2941 caattagtca cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc
     3001 aaccgtcagc agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat
     3061 caggtgatga agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc
     3121 aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt
     3181 gaagaacagc gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc
     3241 gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg
     3301 gggtgtgcgc tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg
     3361 cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa
     3421 gtcaccggtc agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc
     3481 aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg
     3541 tctaaacgtc gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc
     3601 gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac
     3661 gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt
     3721 tatctggaga aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac
     3781 cacgttaacg acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg
     3841 tgtaagatca acctgatccc gtggaacccg ttcccggacg cgccgtatgg acgcagttcg
     3901 aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg
     3961 cgtaaaacgc gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc
     4021 gaccgtacga aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg
     4081 gtctgatagc cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga
     4141 agtattaagt tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca
     4201 gtatgtaacg atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac
     4261 agacggtctt atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag
     4321 catgatggtt caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg
     4381 aagccacgca cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg
     4441 aacaactagg acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg
     4501 tacgcgacat tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat
     4561 atatccgctc ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg
     4621 aaaagcaggc tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta
     4681 aacgccgcaa aaaacgcgac ggctggctaa tgaccttcac ctggctggtg ttgtttgtgg
     4741 ttatcggcct gagcggtgtc tggtggtggc aagaccacaa agctcagcag gaagagatca
     4801 ccactatggc cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg
     4861 ttccgttaaa tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg
     4921 tggatactac cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg
     4981 atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc
     5041 cggtaccaac ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg
     5101 gcgtgaccac gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct
     5161 ggctggaggt cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg
     5221 gtaatttgaa tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag
     5281 tacagatcca gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg
     5341 ttgcgcgtct gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg
     5401 gagatttttc atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt
     5461 tgggaatgtg ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg
     5521 tacgacagat gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga
     5581 tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca
     5641 gcgggttaac gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt
     5701 agcggaatac ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg
     5761 tattcgcatg gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa
     5821 cgccggatcg ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt
     5881 gctggaatcc gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa
     5941 agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa
     6001 acagatcgat cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc
     6061 agtaaaatcc gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg
     6121 tgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc
     6181 gctgcgtatc cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga
     6241 atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc
     6301 gatggacgtt tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac
     6361 actcggcgtc accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga
     6421 ccgtctggac aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag
     6481 tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt ggaaaaataa taacgtgatg
     6541 ggaagcgcct cgcttcccgt gtatgattga acccgcatgg ctcccgaaac attgagggaa
     6601 gcgttgaggg ttcattttta tattcagaaa gagaataaac gtggcaaaaa acattcaagc
     6661 cattcgcggc atgaacgatt acctgcctgg cgaaacggcc atctggcagc gcattgaagg
     6721 cacactgaaa aacgtgctcg gcagctacgg ttacagtgaa atccgcttgc cgattgtaga
     6781 gcagaccccg ctattcaaac gtgcgattgg tgaagtcacc gacgtggttg aaaaagagat
     6841 gtacaccttt gaggatcgca atggcgacag cctgactctg cgccctgaag ggacggcggg
     6901 ctgtgtacgc gccggcatcg agcatggtct tctgtacaat caggaacagc gtctgtggta
     6961 tatcgggccg atgttccgtc acgagcgtcc gcagaaaggg cgttatcgtc agttccatca
     7021 gttgggctgc gaagttttcg gtctgcaagg tccggatatc gacgctgaac tgattatgct
     7081 cactgcccgc tggtggcgtg cgctgggtat cttcgaacac gtaactcttg agctgaattc
     7141 tatcggttcg ctggaagcac gcgccaatta ccgcgatgcg ctggtggcat tccttgagca
     7201 gcataaagaa aagctggacg aagactgcaa acgccgcatg tacactaacc cactgcgcgt
     7261 gctggattca aaaaatccgg aagtgcaggc gcttctcaac gacgctccgg cattaggtga
     7321 ttatctggac gaggaatctc gtgagcattt tgccggtctg tgcaaactgc tggagagcgc
     7381 ggggatcgct tacaccgtaa accagcgtct ggtgcgtggt ctggattact ataaccgtac
     7441 cgttttcgag tgggtgacta acagtctcgg ctcccagggc accgtgtgtg caggcggtcg
     7501 ttatgacggt cttgtggaac aactgggcgg tcgtgcaaca ccggctgtcg gttttgcgat
     7561 gggcctcgaa cgtcttgtat tgttagtaca ggccgttaat ccggaattta aagccgatcc
     7621 tgttgtcgat atatacctgg tggcttcagg tgctgataca caatctgcgg ctatggcatt
     7681 agctgagcgt ctgcgtgatg aattactggg cgtgaaattg atgaccaacc acggcggcgg
     7741 caactttaag aaacagtttg cccgtgctga taaatggggt gcccgcgttg ctgtggtgct
     7801 gggtgagtct gaagtggcta acggcacagc agtagtgaag gatttgcgct ctggtgagca
     7861 aacggcagtt gcgcaggata gcgtagccgc gcatttgcgc acgttactgg gttaaggaag
     7921 gagaaggaca gcgtggaaat ttacgagaac gaaaacgacc aggtagaagc ggttaaacgc
     7981 ttttttgctg aaaatggcaa agcactggct gttgggggga ttttgggcgt tggcgcactg
     8041 attggctggc gctactggaa cagccatcag gttgattctg cacgctccgc ttctcttgcc
     8101 tatcaaaatg cggttaccgc agtgagcgaa ggcaaaccgg atagcatccc ggcggcggaa
     8161 aaatttgctg ctgaaaataa aaatacttat ggtgcgctgg cttctttgga acttgcgcag
     8221 caatttgttg acaaaaatga actagagaaa gctgccgccc agttacaaca ggggctggca
     8281 gacacgagcg atgaaaatct caaagccgtg ataaatctgc gtcttgctcg cgttcaggta
     8341 cagctcaagc aggctgatgc cgcgctgaaa acccttgata ccatcaaagg tgaagggtgg
     8401 gctgccattg ttgccgactt gcgtggtgaa gcattgctga gcaaaggtga taagcaaggt
     8461 gcgcgtagtg catgggaagc aggcgtgaaa agcgatgtta ctccggcact gagcgaaatg
     8521 atgcagatga aaattaataa tttgtccatc tgagagggac ccgatgcaat tgcgtaaatt
     8581 actgctgcca ggactgcttt ccgttaccct tttaagcggc tgttcgctgt ttaacagcga
     8641 agaagatgtg gtaaagatgt ccccattgcc aaccgttgaa aaccagttta cgccgaccac
     8701 ggcgtggagc acttccgttg gtagcggcat tggcaacttc tattccaatc ttcatccggc
     8761 actggcggac aacgttgtct atgcagcgga ccgcgctggt ttggtaaaag cgctgaatgc
     8821 ggatgatggc aaagaaatct ggtctgtcag cctggccgag aaagatggct ggttctctaa
     8881 agagcctgca ttactttctg gcggtgtgac cgtgtctggt gggcatgtct acattggcag
     8941 cgaaaaggcg caggtttacg cgctgaatac cagcgatggt actgtggcat ggcaaactaa
     9001 agtcgcgggt gaagcacttt cgcgcccggt ggtcagcgac ggtctggtgt taatccacac
     9061 cagtaacggt cagttacaag cgctgaacga agctgacggc gctgtcaaat ggacagttaa
     9121 cctcgatatg ccttcgctct ctttgcgtgg cgagtctgcg ccggcaacgg cttttggtgc
     9181 ggccgtcgtg ggtggcgata atggtcgcgt cagcgcagtg ctgatggaac agggccagat
     9241 gatttggcag cagcgtattt cccaggcgac cggttctacc gaaattgacc gtctgagcga
     9301 tgttgacacg actcccgtcg ttgttaacgg cgttgttttc gcgctggcct ataatggtaa
     9361 cctgacggcg cttgatctgc gcagtggtca gattatgtgg aaacgcgaac tgggttcggt
     9421 gaatgatttc atcgtcgatg gcaatcgcat ctatctggtc gatcaaaatg accgggtgat
     9481 ggcgttgacc attgatggcg gcgttacgct gtgggcacaa agcgatctgc tgcatcgcct
     9541 gctgacttct ccggtgctgt ataatggcaa cctggtggtc ggtgacagtg aaggttatct
     9601 gcactggatt aacgtcgaag atggtcgttt cgttgcccag caaaaagttg atagttccgg
     9661 tttccagact gaaccggttg ccgctgacgg caaactgctg atccaggcaa aagacggaac
     9721 cgtgtactct attacacgtt aatcgtctct gtcgttcact ttgaaaacgg ctcctggaca
     9781 ggggccgttt tcctgttttt aacaacgacg cgaatatagc gtgcgttgtc tgatgattta
     9841 taaaaatgag gctttaaaca tggtacctgt ggtcgcgctt gtcgggcgcc ctaacgtagg
     9901 aaaatccacg ttatttaacc gtctaactcg cacccgagat gcgctggttg cggatttccc
     9961 gggtctgact cgtgaccgta agtacggtcg tgcggaaatt gaaggccgtg agtttatctg
    10021 tattgatacc ggcgggattg atggcacaga agacggtgta gaaacccgca tggcggaaca
    10081 gtcgctgctg gcgattgaag aagcggacgt cgtactgttt atggtggatg cgcgcgcggg
    10141 cctgatgccg gcagatgaag cgattgccaa acatctgcgc tcccgtgaaa aaccgacctt
    10201 cctggtggta aacaaaactg acggtctgga tcccgatcag gcagtggttg atttctactc
    10261 gcttggttta ggtgaaatct acccgatcgc cgcgtctcac ggtcgtggcg tattaagtct
    10321 gctggagcat gtgctgctgc cgtggatgga agatctcgca ccgcaagagg aagtcgacga
    10381 agacgctgaa tactgggcgc aatttgaagc ggaagagaac ggcgaagaag aagaggaaga
    10441 cgacttcgac ccgcaaagtc tgccgatcaa actggcgatt gtgggtcgtc cgaacgtagg
    10501 taagtctaca ctcactaacc gtattcttgg agaagagcgc gttgttgttt acgacatgcc
    10561 tggcacgacg cgtgacagca tctacatccc aatggaacgc gatggacgtg agtatgtgct
    10621 cattgacacc gctggcgtac gtaaacgcgg caaaatcacc gatgctgtag agaaattctc
    10681 cgtaatcaaa acgttgcagg ccattgaaga cgccaacgtg gtgatgttag tgattgatgc
    10741 gcgcgaaggt atttccgatc aggatctctc gctgctgggc tttattctca atagtgggcg
    10801 ctcacttgtc attgtggtga ataagtggga tggcctgagc caggaagtga aagagcaggt
    10861 gaaagaaacg ctggacttcc gtctgggctt tatcgatttt gctcgtgtgc actttatctc
    10921 tgccttgcac ggcagtggtg ttggtaactt gtttgaatca gtacgtgaag cgtatgacag
    10981 ctccacccgt cgtgtgggga cctctatgct gacgcgcatc atgacgatgg ctgttgaaga
    11041 tcaccaaccg ccgctggtac gcggtcgtcg tgtgaagctg aaatatgccc acgctggtgg
    11101 ttataacccg ccgattgtgg tgattcacgg taatcaggtg aaagacctgc ctgattccta
    11161 caagcgctac ttgatgaact acttccgcaa atcgctggac gtaatgggat cgccgattcg
    11221 tattcagttc aaagaagggg aaaacccgta tgcgaacaag cgtaacaccc tgacgccaac
    11281 ccagatgcgt aaacgtaagc gtctgatgaa gcacatcaag aaaaataaat aattatccgt
    11341 aagtatttta tctgccattc cctctatgaa tgtagaggga atggctaccg gaggaattca
    11401 tatggaactg cattgtccaa aatgtcagca tgtgcttgat caggataacg gccatgcgcg
    11461 ttgcccatcc tgcggggaat ttattgaaat gaaagctctt tgtccagact gccatcaacc
    11521 gttgcaggtg ttaaaagcct gcggtgcagt agattacttc tgtcagcacg ggcatggctt
    11581 gatatcgaaa aaacgcgttg aattcgtgct ggcttaatga acctttttac gcgatttttt
    11641 taccggctgg atgtttttta cttcactttc tatccagccg tcttccagac gtgtggttag
    11701 catttcaccc gttttaactt gcttcacttt tttcagtaca ttgccgtcag tagcagtagt
    11761 aacgctgtat ccacgcgcca gcgttgacag tgggcttacg gcttcgaggt gcgttactgc
    11821 attaccgaaa cgttcacgcg tggcgctaag ctgtgcgcgc aggatttctg ctaaacgata
    11881 ttccagttgc tgaatgcgcg tttgcgtgcg atgaatcttc ggctgtggat tttgctgatt
    11941 cagccgctgt gttaatcgct gctgctgttg cccggcacgc ttaagctgat tttccagcgc
    12001 aaagctcatc cgtttttgca ggcgttcaag catggtttgc tggcgtgcca gccggagctg
    12061 cggatgctgc tgctgcaagc gatgatgaat ctgcgtaaag cgacgtgtac ggttggcgag
    12121 ataataatcc atcgccatct ccagccgttg acgggccgat tgcacctggc gcagtaactc
    12181 ttgctgatta cggctcacta cttcagcggc ggcagacggc gttggcgcac gcagatcggc
    12241 aacaaaatcg gcaatggtca catccgtctc atgcccgacg gcgctgacga ccggaatgcg
    12301 gctggcaaaa atcgcccgcg ctacgcgttc gtcgttaaaa ctccataaat cttccagcga
    12361 accgccgccg cgcccaacga tcaacacgtc gcactcattg cgctgattcg ccagttcaat
    12421 ggcgcgaaca atttgccccg gcgcgtcatc gccctgaacg gaggtggggt agatgatcac
    12481 cggtagagaa ggatcgcgac gttttaacac atgcaaaata tcatgtagcg cagcaccggt
    12541 ttttgaggtg atcacaccaa cgcaatgcgc aggggaggga agtggttttt tgtattgctg
    12601 atcgaacaaa ccttcagcct gcaacttcgc tttgagctgt tcgtacttct gttgcagcag
    12661 cccttcaccg gccggctgca tactctcaac gattatctgg tagtcgccgc gcggctcgta
    12721 gagcgtaata ttggcgcgaa ctaaaacttg ttgcccatgc tgcggacgga aggtgacccg
    12781 gcggttgctg ttgcggaaca tcgcgcagcg tacctgggcg gtgtcgtctt tgagtgtaaa
    12841 gtaccagtga ccggaagccg gttgtgtgaa attagagatt tcgccgctga tccaaacctg
    12901 tcccatctca tgctcaagca gcagacgaac cgtttgattc aggcgactaa cggtaaaaat
    12961 tgcaggggat tgagaaggta acatgtgagc gagatcaaat tctaaatcag caggttattc
    13021 agtcgatagt aacccgccct tcggggatag caagcatttt ttgcaaaaag gggtagatgc
    13081 aatcggttac gctctgtata atgccgcggc aatatttatt aaccactctg gtcgagatat
    13141 tgcccatgct acgtatcgct aaagaagctc tgacgtttga cgacgttctc ctcgttcctg
    13201 ctcattctac cgttctgccg aatactgctg acctcagcac ccagctgacg aaaactattc
    13261 gtctgaatat ccctatgctt tccgcagcaa tggataccgt aacggaagcg cgcctggcta
    13321 ttgctctggc tcaggaaggc ggtatcggct ttatccacaa aaacatgtcc attgaacgcc
    13381 aggcagaaga agttcgccgt gtgaaaaaac acgaatctgg tgtggtgact gatccgcaga
    13441 ctgttctgcc aaccacgacg ctgcgcgaag tgaaagaact gaccgagcgt aacggttttg
    13501 cgggctatcc ggtcgttacc gaagaaaacg aactggtggg tattatcacc ggtcgtgacg
    13561 tgcgttttgt taccgacctg aaccagccgg ttagcgttta catgacgccg aaagagcgtc
    13621 tggtcaccgt gcgtgaaggt gaagcccgtg aagtggtgct ggcaaaaatg cacgaaaaac
    13681 gcgttgaaaa agcgctggtg gttgatgacg aattccacct gatcggcatg atcaccgtga
    13741 aagacttcca gaaagcggaa cgtaaaccga acgcctgtaa agacgagcaa ggccgtctgc
    13801 gtgttggtgc tgcagttggc gcaggtgcgg gtaacgaaga gcgtgttgac gcgctggttg
    13861 ccgctggcgt tgacgttctg ctgatcgact cctcccacgg tcactcagaa ggcgttttgc
    13921 agcgtatccg tgaaacccgt gctaaatatc cggatctgca aatcatcggc ggcaacgtgg
    13981 caacagctgc aggtgcacgc gctttggcag aagctggttg cagcgcggtt aaagtcggta
    14041 tcggccctgg ttctatctgt acgactcgta tcgttactgg cgtaggtgtt ccgcagatca
    14101 ctgccgttgc tgacgcagtt gaagccctgg aaggcaccgg tattccggtt atcgcagatg
    14161 gtggtattcg cttctccggc gacatcgcca aagctatcgc cgctggcgca agcgcggtga
    14221 tggtaggttc catgctggcg ggtactgaag aatctccggg tgaaatcgaa ctctaccagg
    14281 gccgttctta taaatcttac cgtggtatgg gttccctggg cgcgatgtcc aaaggttcct
    14341 ctgaccgtta tttccagagc gataacgctg ccgacaaact ggtgccggaa ggtatcgaag
    14401 gtcgcgtagc ttataaaggt cgcctgaaag agatcattca ccagcagatg ggcggcctgc
    14461 gctcctgtat gggtctgacc ggctgtggta ctatcgacga actgcgtact aaagcggagt
    14521 ttgtacgtat cagcggtgcg ggcattcagg aaagccacgt tcacgacgtg accattacta
    14581 aagagtcccc gaactaccgt ctgggctcct gattctcttc gcccgatttc atgtcgggcg
    14641 atttatatta tctgtttcac ttgcctcgga ataagcgtca atgacggaaa acattcataa
    14701 gcatcgcatc ctcattctgg acttcggttc tcagtacact caactggttg cgcgccgcgt
    14761 gcgtgagctg ggtgtttact gcgaactgtg ggcgtgggat gtgacagaag cacaaattcg
    14821 tgacttcaat ccaagcggaa ttattctttc cggcggcccg gaaagtacta ctgaagaaaa
    14881 cagtccgcgt gcgccgcagt atgtctttga agcaggcgta ccgatattcg gcgtttgcta
    14941 tggcatgcag accatggcaa tgcagttggg cggtcacgtt gaagcctcta acgaacgtga
    15001 atttggctac gcgcaggtag aagtcgtaaa cgacagcgca ctggttcgcg gtatcgaaga
    15061 tgcgctgacc gcagacggta aaccgctgct cgatgtctgg atgagccacg gcgataaagt
    15121 taccgctatc ccgtccgact tcgtcaccgt agccagcacc gaaagctgcc cgtttgccat
    15181 tatggctaac gaagaaaaac gcttctatgg cgtacagttc cacccggaag tgactcacac
    15241 ccgccagggt atgcgcatgc tggagcgttt tgtgcgtgat atctgccagt gtgaagccct
    15301 gtggacgcca gcgaaaatta tcgacgatgc tgtagctcgc atccgcgagc aggtaggcga
    15361 cgataaagtc atcctcggcc tctctggtgg tgtggattcc tccgtaaccg caatgctgct
    15421 gcaccgtgct atcggtaaaa acctgacttg cgtattcgtc gacaacggcc tgctgcgtct
    15481 caacgaagca gagcaggttc tggatatgtt tggcgatcac tttggtctta acattgttca
    15541 cgtaccggca gaagatcgct tcctgtcagc gctggctggc gaaaacgatc cggaagcaaa
    15601 acgtaaaatc atcgggcgcg ttttcgttga agtattcgat gaagaagcgc tgaaactgga
    15661 agacgtgaag tggctggcgc agggcaccat ctaccctgac gttatcgaat ctgcggcttc
    15721 tgcaaccggt aaagcacacg tcatcaaatc tcaccacaac gtgggcggcc tgccgaaaga
    15781 gatgaagatg ggcctggttg aaccgctgaa agagctgttc aaagacgaag tgcgtaagat
    15841 tggtctggag ctgggcctgc cgtacgacat gctgtaccgt catccgttcc cgggaccagg
    15901 ccttggcgtt cgtgttctgg gtgaagtgaa gaaagagtac tgtgacctgc tgcgccgtgc
    15961 tgacgccatc ttcattgaag aactgcgtaa agcggacctg tacgacaaag tcagccaggc
    16021 gttcaccgtc ttcctgccag tacgttccgt tggcgtaatg ggcgatggtc gtaagtatga
    16081 ctgggttgtc tctctgcgtg ctgtcgaaac catcgacttt atgaccgcac actgggcgca
    16141 tctgccgtac gatttcctcg gtcgcgtttc caaccgcatt atcaatgaag tgaacggtat
    16201 ttcccgcgtg gtgtatgaca tcagcggcaa gccgccagct accattgagt gggaatgatt
    16261 ttgatccctg cactatgaat gaacaaaacc ctccgttact acagagggtt ttttatcttc
    16321 aagaatcata ggcttgaagt tactaacatc gattaattaa accagctgtc cgatttgttc
    16381 tcttctgctt tgcccacgct tttcatcaga tcgcgaccgc cttcagtcat atttctgttt
    16441 gcgtcagctt cagattgcac cacatcggtt tgcgcagctt tgtgcttcag ttcctgatcg
    16501 ataaattcgt tttcgcgctt aacgcgggct tcttctttcg ccagcgccag tttttgtttc
    16561 tgaatctcta agctgcgtag ctcatcttca taactttgat cgcgtttttt gtccgcagtg
    16621 gcttcggcgt ccagtttatc ctgacgagct ttcttatttg ccgctgccgt tgccgctctt
    16681 ttgttcgcgg cggcctgggc atttgcgcga cgttgcttct cttgctggat ttccctgttg
    16741 cgctccgcga cccattcgtc atgctgcctt tgctcttcat ttttaccttg ctgttccgct
    16801 tctgcgacag cagagagttg atcctgcaat gatgaggcta tagccggata gcttaaggag
    16861 gctaagatgg cgcaaagaaa aactttcttc atgactcctc ctgattatta gctcttttca
    16921 ggacatttgg tatttggctg aatacgcgtt tcgttatacg ttgtggtaat aacaacggct
    16981 aaacctgtcg taaactggca ctctttaccc tcctgggtgg aggtatacac tttggtgcct
    17041 tccttatatg ttaaagaaac accttccact aaggttttat cattcaccat agaacccgct
    17101 gccgcgccta cagctccgcc gccaactgcc cctgccgtcg ttccggaatt gctgccagaa
    17161 ccgacgttgt ggccgataac accgccagcg actgcgccaa taagcgcgcc gaaggcttgt
    17221 gcgttccgtt tattttggga gttgtctacg gcaacttttg cgggaagaat ggaaataata
    17281 ttaacggttt tagtttcttg tttggtattc agttgatcgg tttgataaac atcggcggca
    17341 tgatcgtcag catttgactg gcatcctgcc agagtgaatg acgctaacat tgccacaggc
    17401 agaagacatt ttttaaattt catcactatt ccttgttata cacattacga aaaataagac
    17461 atttaagatc atcaaaagag gaaagtaaat gtattgcgta taaagggata gattaaattt
    17521 attaaaattc acgcagggaa taatttataa aaatatatca ttacaacata tatattaatt
    17581 ttatatttat ttcgcagcca aacaggataa acatcagtca ggacaaagac gtaaaattta
    17641 agatgaaaga atatctgccg ctttgataat cccaaagagc aaaagcatcc gacagcgaag
    17701 tgccgttatg ttctgtgaaa gtggagaagg taaccgctaa cgttgtaccg gtgattcgac
    17761 ggagagtgtg gattgctgag cttctttttt gctctgatgg tgctgccagg caccgacgga
    17821 cgagtagata aaacggccaa agaagaagat aaagctgatg agcagtacga tacgggtcat
    17881 gcgactgtta aatcggtgtc gttttcgcat actagttgcc tgactcacaa aaggttcctt
    17941 gaagtatgtc ccacgctgtg gacggtactt acattaaggc acaacaggac aaaatggtca
    18001 attcttcgtt atgtaaaaaa gcgtcagtgg atacatattt taatgttatg gaagttaatt
    18061 taattattta caatgatgat gtaataatga tgaattaaca tgataaacgt aataattcat
    18121 taatcagggt tattttgttt gatatatatc aattggaact ttcatatgac acttagaatc
    18181 aatgctctct atagtgatga ataatcatca ttcgaagtca ggtgggatgc ctgtctgaat
    18241 acacctcctt caggatgtgg gggattctgc tgagcatcta tgaaactgaa tgcaacttat
    18301 ataaaaatac gtgataaatg gtgggggctt ccgctgttcc tgccttcttt aatcttgccc
    18361 attttcgccc acattaatac tttcgcgcat atttcttccg gtgaggtttt tctcttttat
    18421 ctgcctttgg cactgatgat cagcatgatg atgtttttca gctgggcggc attgccaggg
    18481 atcaccttag ggatttttgt ccgcaaatat gcagagctgg gtttttacga aacgctctca
    18541 ttaacggcta attttattat cattatcatt ctctgttggg gcggttacag ggtctttact
    18601 ccccggcgta acaacgtttc acatggtgat acccgtttaa tttcccagcg tatattctgg
    18661 cagatagtgt ttcctgcaac gctgtttctg atacttttcc agtttgctgc atttgtagga
    18721 ttactggcga gcagagaaaa tctggtcggt gtcatgcctt ttaacctcgg gactttaatc
    18781 aattatcagg ccttactggt gggtaatctg atcggtgtcc cgctgtgcta cttcatcatt
    18841 cgggtagtgc gaaatccatt ttatttacgt agctattatt cgcaattaaa acagcaggtt
    18901 gatgccaaag tcaccaaaaa agagttcgcg ctctggctac tggcattagg tgctttattg
    18961 ttgctgttat gcatgccgtt aaatgaaaaa agcacaattt ttagcaccaa ttataccttg
    19021 tcattattgc tgcccctgat gatgtgggga gcaatgcgct atggttataa gctgatttcg
    19081 ctgctctggg cggtcgtgtt gatgatcagc atccacagct atcaaaatta cattcccatt
    19141 tatcctggct ataccacgca gctgaccata acctcctcca gttatctggt attctctttt
    19201 attgtcaatt atatggctgt actggcaact cgtcagcgag cggtagtcag acgcattcag
    19261 cggcttgcgt atgtggaccc ggtggttcat ctgccaaatg ttcgcgccct gaatcgcgcg
    19321 ttgcgtgatg ccccctggtc tgcgctttgt tatttacgca tccctggcat ggaaatgctg
    19381 gttaagaact atggcatcat gctgcggatt caat
//
LOCUS       DAEEGA010000078        19375 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEEGA010000078 DAEEGA010000000
VERSION     DAEEGA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19375
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.078"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="KLH46_002960"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="KLH46_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBE0379563.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="KLH46_002961"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="KLH46_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBE0379564.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="KLH46_002962"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="KLH46_002962"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBE0379565.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="KLH46_002963"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="KLH46_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBE0379566.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="KLH46_002964"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="KLH46_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBE0379567.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="KLH46_002965"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="KLH46_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBE0379568.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="KLH46_002966"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="KLH46_002966"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBE0379569.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="KLH46_002967"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="KLH46_002967"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBE0379570.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="KLH46_002968"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="KLH46_002968"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBE0379571.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="KLH46_002969"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="KLH46_002969"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBE0379572.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="KLH46_002970"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="KLH46_002970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBE0379573.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="KLH46_002971"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="KLH46_002971"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBE0379574.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="KLH46_002972"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="KLH46_002972"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBE0379575.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="KLH46_002973"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="KLH46_002973"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBE0379576.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="KLH46_002974"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="KLH46_002974"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBE0379577.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="KLH46_002975"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="KLH46_002975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBE0379578.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="KLH46_002976"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="KLH46_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBE0379579.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="KLH46_002977"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="KLH46_002977"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBE0379580.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4768 a         4858 c         5122 g         4627 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccaagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgctcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggcg caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaagtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgctgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagac aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctgg cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccg
//
LOCUS       DAEEGA010000079        19263 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEEGA010000079 DAEEGA010000000
VERSION     DAEEGA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19263
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.079"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            32..331
                     /locus_tag="KLH46_002978"
     CDS             32..331
                     /locus_tag="KLH46_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBE0379581.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            328..2076
                     /locus_tag="KLH46_002979"
     CDS             328..2076
                     /locus_tag="KLH46_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBE0379582.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPAEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            2365..2643
                     /locus_tag="KLH46_002980"
     CDS             2365..2643
                     /locus_tag="KLH46_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379583.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2640..2945
                     /locus_tag="KLH46_002981"
     CDS             2640..2945
                     /locus_tag="KLH46_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379584.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2955..3116
                     /locus_tag="KLH46_002982"
     CDS             2955..3116
                     /locus_tag="KLH46_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HBE0379585.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            3258..3482
                     /locus_tag="KLH46_002983"
     CDS             3258..3482
                     /locus_tag="KLH46_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379586.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            3783..4940
                     /locus_tag="KLH46_002984"
                     /pseudo
     CDS             3783..4940
                     /locus_tag="KLH46_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            4980..5552
                     /locus_tag="KLH46_002985"
     CDS             4980..5552
                     /locus_tag="KLH46_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBE0379587.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            5554..6765
                     /locus_tag="KLH46_002986"
     CDS             5554..6765
                     /locus_tag="KLH46_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBE0379588.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            6762..7100
                     /locus_tag="KLH46_002987"
     CDS             6762..7100
                     /locus_tag="KLH46_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBE0379589.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7097..7393
                     /locus_tag="KLH46_002988"
     CDS             7097..7393
                     /locus_tag="KLH46_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBE0379590.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            7393..7833
                     /locus_tag="KLH46_002989"
     CDS             7393..7833
                     /locus_tag="KLH46_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBE0379591.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            7817..7999
                     /locus_tag="KLH46_002990"
     CDS             7817..7999
                     /locus_tag="KLH46_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379592.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            8055..8345
                     /locus_tag="KLH46_002991"
     CDS             8055..8345
                     /locus_tag="KLH46_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBE0379593.1"
                     /translation="MDDIAVKQWREKSRQLAEQGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPP"
     gene            8365..10026
                     /locus_tag="KLH46_002992"
     CDS             8365..10026
                     /locus_tag="KLH46_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBE0379594.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            10040..10321
                     /locus_tag="KLH46_002993"
     CDS             10040..10321
                     /locus_tag="KLH46_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379595.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(11118..12578)
                     /gene="phoQ"
                     /locus_tag="KLH46_002994"
     CDS             complement(11118..12578)
                     /gene="phoQ"
                     /locus_tag="KLH46_002994"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBE0379596.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(12578..13249)
                     /gene="phoP"
                     /locus_tag="KLH46_002995"
     CDS             complement(12578..13249)
                     /gene="phoP"
                     /locus_tag="KLH46_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBE0379597.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(13418..14788)
                     /gene="purB"
                     /locus_tag="KLH46_002996"
     CDS             complement(13418..14788)
                     /gene="purB"
                     /locus_tag="KLH46_002996"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBE0379598.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(14792..15433)
                     /gene="hflD"
                     /locus_tag="KLH46_002997"
     CDS             complement(14792..15433)
                     /gene="hflD"
                     /locus_tag="KLH46_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBE0379599.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(15469..16575)
                     /gene="mnmA"
                     /locus_tag="KLH46_002998"
     CDS             complement(15469..16575)
                     /gene="mnmA"
                     /locus_tag="KLH46_002998"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBE0379600.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(16629..17090)
                     /gene="nudJ"
                     /locus_tag="KLH46_002999"
     CDS             complement(16629..17090)
                     /gene="nudJ"
                     /locus_tag="KLH46_002999"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBE0379601.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(17100..17753)
                     /gene="rluE"
                     /locus_tag="KLH46_003000"
     CDS             complement(17100..17753)
                     /gene="rluE"
                     /locus_tag="KLH46_003000"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBE0379602.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            17925..19175
                     /gene="icd"
                     /locus_tag="KLH46_003001"
     CDS             17925..19175
                     /gene="icd"
                     /locus_tag="KLH46_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBE0379603.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         5016 a         4728 c         5159 g         4360 t
ORIGIN      
        1 ataaagaaca acacaccgga gaataaaaat aatgaaactt aaatattctg gcttaactgc
       61 cagtggcaac actcacccta aatttacgcg cggtgatatt taccgcgacc agtacggcgg
      121 catggtaacg attaaaggcg tggcagaacg gcgcatcacc taccgccgtg aaggttacga
      181 atatgattgc gtgatgcctg tttatcaatt ccggcgtgat ttcacgctgg tacaggcagc
      241 gccccgcagt aaaccaacca gcagggagaa agcacgcgcc aatattcagg aaataaaaaa
      301 gatgcttaac gtattcaggg gtaaaaaatg aaactggcac cgaacgtaaa aaaacagcca
      361 cgcggaataa aacacaaaga cacagaggta attatctttg cgggtagtga tgcctggtcg
      421 cacgcgaaac aatggcagga gcaggatggc cccgcatccg gcgataatgt gccgcctgtg
      481 tggcttgggc caaatcagct tgccgaactt gatgcactga aaattgttcc ggatggaaaa
      541 aaacgcgtaa ggctgtacca ggccggagaa ctggatttgg tggagaccaa aaagattggt
      601 cagaagctgg cggcggcaga tattcaggac gcaaattttt accccgaagg aatgcacgtc
      661 cagaagtgtg aaaactggcg gcgctatctg aatgctgagc gtgaaaatat tgccgcaggg
      721 cttaccatgc cggagcagaa aaatacgcaa ctggcacaaa tggcagacag tgagcgcgcg
      781 cagctgcttg ccgagcgctt tgatggcgtt tgtgtgcatc aggaaagtga aatcgttcac
      841 gtatggcgcg gcggggtatg gtgtccggtc agcacaatgg aacttagccg cgaaatggtg
      901 gcgatctatt cagagcacag ggccactttc agcaagcgcg taatcaataa cgccgtggaa
      961 gcgttaaaag ttattgccga acccatgggc gagccgtccg gcgatttgct gccgttcgcc
     1021 aatggtgcgc ttgacctgaa aacgggggaa ttttccccgc acacgccgga gaactggatc
     1081 accacgcaca acggcattga gtacacgcca ccagcaccag gggagaacat ccgcgataac
     1141 gcgctaaact ttcataaatg gcttgagcac gcagccggaa aagaccagcg caagatgatg
     1201 cgtatatgtg ccgcgctgta catgattatg gcgaaccggt acgactggca gatgtttatt
     1261 gaggccaccg gagacggcgg gagcggtaaa agtacattca cacacatagc cagccttctg
     1321 gcagggaaac agaacacggt aagcgctgaa atgacatcgc ttgatgatgc tggtggacgt
     1381 gcgcaggttg tcgggagtcg tcttatcgtc ctggctgacc agccgaaata tacaggcgaa
     1441 ggaacgggca tcaagaaaat cacgggcggt gaccccgcgg aaattaaccc gaaatacgaa
     1501 aagcgtttca cggctgtaat cagggcggtg gtgctggcga ccaacaataa cccgatgata
     1561 ttcaccgaac gggccggagg tgtggcacgt cgtcgcgtga ttttccgttt cgacaatatc
     1621 gttagtgagg ccgaaaaaga cagggagcta ccggaaaaga ttgcggctga aatccctgtc
     1681 attatccgcc gattgctggc gaactttacc gactctgaga aagcacgggc tttactactg
     1741 gaacagcgtg acggtgatga agctctggca ataaagcaac agacggatcc ggttattgag
     1801 ttttgccagt tcctgaattt tctggaggaa gcacgcggcc tgatgatggg cggcggtggc
     1861 gattcagtga agtacacgac cagaaacagc ctttaccgcg tctatctggc gtttatggcg
     1921 tacgcaggca ggagcaaacc gctaaacgta aatgactttg gcaaggctat gaagccagcc
     1981 gcgaaagttt acggacatga atatattacg cggaaagtta agggagtaac gcagactaac
     2041 gcaataacaa cagacgagtg cgacgcgttt ttataatttt ttgtaattgc tgtctaccct
     2101 gtctacctga gtaaagaaaa atacatttaa ttcagtgcat taagttaggt agatagcctt
     2161 tttttactgt ctacccacta tctaccctct ctacctgatt ttacctgaat cagacaggga
     2221 ggtagacacg gggtagatag tggataaaag cactctaccc cgctgaaagc agcgccatta
     2281 ctggcatgag ggccactaag gtagataagg tagacaaggg gaggcacaac tcaaaacttt
     2341 ttaaacgagg gggtaaaaat aaatatgcac acttcaggga aatttaataa atcattcaaa
     2401 aaacgcagag acagaacaga accgaaatat cgcgcgttag acatgacaga gcacgcttta
     2461 aaggtggcaa tcagaacgat agaccgccac gcgggggaag gatacgcgaa agcacatccc
     2521 gaactgataa gcgcattcat gaccacgacg gcggcaaatt ttgccacgct gacagagcgg
     2581 gagattgccg aagcggaaca ggtaacaaca atcaacgtta aaaccggaga ggtggaatca
     2641 tgagcgaacc aaaatttggt gaaaaatttt acaagcacaa cggacggatc acgattctgc
     2701 aaataagcgc agctacacca ggctggtggg ttgaaacaga cgaaggatcc tcacccgtgg
     2761 catcatgggc gctttgtgtg cttagttacc ctgaccgcga tgtgtatcag gatattctgc
     2821 ctgtgatatc cactgacaaa ggcatgaaac ccgtagacat caaaaacatg gggtttcagt
     2881 gcgtaatgct cactgaaaaa atgatggaag agatgaaaaa gaacaatgcc ggatcgcttc
     2941 actgaggtgg caatatgaca gcacaaattg cagcttacgg gcggctggtg ggcgacccgc
     3001 tggtaaaaca gagcagcaag ggcacaccgg agccgccaac ggagaactac gcagacctga
     3061 gaaatgcagt aaatcgcact tacgcagaca tggggctgag caaattagca gagtaggaat
     3121 atcagaacca caccacgcag ccggagcaat ccggcttttt ttgcgtcatt tgaaacggta
     3181 tggagcacta ctggcttttc gtcacggtcc ggcatagtta ctatctgaaa caaacagaca
     3241 caacagagga aaacgcaatg ccgatgaaat ttgatgagat acttaaacag cgtgataaat
     3301 accatgctga caacatggag acgatgaata tcactgatta ccgcgcattt ctggaaacgg
     3361 gggcgctgat tgaaaaggat catcatggtt ttgttcgttg cgcgctatcc ggtgaaatgc
     3421 tggcagtaaa tcctgaacag acagatgcat tgatagaatt tctgaaaggg atcagagact
     3481 gagcacccat acagcacata cagccggagc aatccggctt tttgttattt tttgtaaatt
     3541 atttgttcgt ggttgttcca cgttgttcac tgagcggatc ggcatatttt acccgaactg
     3601 aatcatgatt attctcgccc gtggtgccag gacgctgggg ccactttccc gcctgttaat
     3661 gtgctcgcca atattcatta ccaggcggga aaacgatcgg tgcgattact gatttcctta
     3721 tgaaaaacgg ttgagtgttt gccgcgtcct ggagttcctt acttaacccc aggacttttt
     3781 ttatgcagag aataatcgaa ttacgccagc agaaaaccgc cattaaaaat cagatgcgcg
     3841 acatgctgga gaacgctgaa aaagaaaacc gcagtcttaa cgatgctgag ggcacaaaat
     3901 ttgacgaatt acgcgctaaa gctgaatccc tcgataaaga catttcccgc cttgaagcca
     3961 ttgcagacga agagcgcagc aagccaggta aaagcagcca gaccactgac cccgcagaac
     4021 tacgcaacta cattctgacc ggtgaaaccc gcgcattaag tacaggcgtt cccgctgatg
     4081 gtggttatac cgttatccct gaactgaaca ccgaaatcat gcgaatgctg gcggatgaat
     4141 ccaccatgcg tcgcatctgt accgtgaaga aaatcagcag caacgagttt aagcagcttg
     4201 tttccgctgg cggtgcgacc gttaaccacg gtgaagaggg taaggcacgc gaacagacca
     4261 gcaccccgca gattaacgag gtgagcatta agctgtatcc ggtctatgcg tacccgcgca
     4321 ccacacagga aatcgtggat ttttccgatg tggacatcct ttcatggctg acgggtgaga
     4381 ttggcgacac cttcacggaa accgaagaaa gcgatctggt tgtgggcgac ggtgacaaaa
     4441 aagcaaaagg ttttttatcc gtaccccgtg cagagaagaa cgacaaagag cgtgattttg
     4501 gtacgttgca ggtaattaaa ccttccgaat ctctggcgtg gacatctgcg gacccgctga
     4561 tcgacctgaa atttgcatta cgtaaaaaat accgcaaaaa cgcggtctga gtggttaact
     4621 ccacgacggc ggcaaaactt cagaaggtga agaacgcgaa cggtgattat atctggcgcg
     4681 accgtttaca gacgggtgat cctgatacgt tgctgggcct tccggtcgaa tatctggagt
     4741 ttatgcctga taacgttatt gccctgggtg acttcaaacg cggttactac attgttgatc
     4801 acgaaacagg tgttcgcacc agaccggaca acctgacaga accaggattc atcaaaattt
     4861 tcacgcaaaa atatttaggc ggtggcgtgg tggattcgaa cgcgatcaag attctggcac
     4921 tgccggaaga cgacgattaa cagcatacag aaggggctaa aaagcccctt tagtgtttta
     4981 tgggtgaaaa aattatgaag agtatggaaa tccggtcatc ggaaatcacc accagcggag
     5041 ccggtacgct gacgggctac gttgttcgct gggataagct ttcagaactg ttatggggtg
     5101 agttttacga aaaattccag cggggggcgt ttactgaatg gcttgcggcg ggtaatgacg
     5161 ttcgcggcct gtatgagcat gaccacagca tgttactggg gcgcacccgt tccggcacgc
     5221 tgaaactgga agaggacgaa acagggttac gctttgaact gaccccaccg gataccagta
     5281 cagggcgcga cgttatcgaa ctggttaagc gcggggacat ctcggggatg agctttggct
     5341 ttcgctcccg taaggatgta tgggatacca caacagatcc atgcgtgcgc accgtgctgg
     5401 tggcggaact gtacgaaatt accgttacat cggtaccggc ttaccccgat tccggcgtgg
     5461 agctggcccg ccgttccctg tatgagcagc accccgaaaa aatgccgcgt gcggataatc
     5521 gccgctggtg ggcggattta gcgggggtgt gatatgtggc ctttcagacg aaaaaaagag
     5581 cagcgcagca tgacgcttga tgaatttatg gcgctggccg gcacatcgaa cacgggggcg
     5641 ggtgagtacg tatcatctgg gacagcggaa tcactccccg ccgtcatgaa cgccgtcacg
     5701 gtcatctctg acgcggtggc taccatgccg tgttacctgt acctggtacg caatgagaag
     5761 gggaaggagg cccgcgaatg gcttgattct catccggtcg atcacatcct caacgagcgc
     5821 ccgaacgcgt ggcagacacc ctaccagttt aagcgaatga tgatccgcta ctgcctgtta
     5881 agtggtaatg cttatgcggt tattcagtgg gggcgtgatg gttttccggc ggctttacat
     5941 ccttacccgc cgcagtcggt gaacgtggag cagacaggtg atcataactg gcgctactgc
     6001 atcactgacg cctacaccgg aaacacccgc aactatttac cgtgggaggt tctccacctt
     6061 cgttactcca cggatgacgg ttttatgggg cgctcacctg taaccatctg ccgcgaatcg
     6121 ctggggcttg ggctggccca acaacgccac ggcgcgagcg tgatgcgtga tggcatgatg
     6181 gcggcagggg ttatcacgtc aggcgaatgg ctggacggcg tgaagggcaa acaggcatta
     6241 gccgcactgg aacgctacaa aggggccaga aacgccggaa aaacgcccat ccttgaaggg
     6301 gggatgagct atcagcagct gggcatgagt aatcaggatg ccgaatggct ggcctcccgt
     6361 cgcttcacca ttgaagacat cgcccgaatg ttcaacgtct cgccgatttt tttgcaggaa
     6421 tacagcaaca gcacctacag caacttcagc gaggcaagcc gcgcatttct caccatgacg
     6481 atgcgcccgt ggctggcgaa ctttgagcag cagataaaaa acgccttgct ggtggcctcg
     6541 cctgtacctg gtatccggta tcaggtggag tttgacagcg cggacctgtt acgggccaca
     6601 cctggcgaac gctttgccac ctatgagcgc ggcataaaat ccgacgttat gtgtccgaac
     6661 gaagcccgcg aacgtgaagg gctgtcaccg cgtgatggtg gtgatgagtt cagccaggca
     6721 tggaaacagg aagtaaaaat cagcgaggga gaaaaaccgg aatgaacata gggcgactgc
     6781 gtgacaggat aacgattcag accctgaaac agaccaggga tataaccggc gaaatactcg
     6841 aaacgtggga ggacggtcac acactctggg caagcgtgaa catggtcagc agcaaggagg
     6901 ccatttcatc gggcgcagag ctggcgattg gtaccgtaag gatctggata cggtaccgga
     6961 aggacatcaa cgccaccagc cggataaagg tcagtacggg gccgctggcg gggcgtgtac
     7021 tgaatatcat cgggcagccg ctgccggatg ccgccaggac acgccttgaa attctttgtc
     7081 gtgagggcgc ggaaaaatga cagaagaact tatcaccctg gaagaagtga aactccattg
     7141 ccgcatcgat ggcgacgatg aagaccagtt aatcagcgga tacattgccg catcgcttga
     7201 ggcgtgccag atacacatag gcaggcgctt tgatgacggg ctggcgttca cgccagccat
     7261 aaagattggc tgcatgatgt ttatcgctca cctgtatgaa aatcgccaga ttgtcgcgga
     7321 taacgcaaaa acacgcgtac ccatgacgat aggcgcactc tggacggctt accgtgatgt
     7381 gggggggtac tgatgccgtg gcaaccatta agacgatgca cagaaccagg ctgtaacagg
     7441 cgcgttaaat ccggcaagtg tgaggagcac aggcgggcgg catggcgtgc agaggatgcc
     7501 agacgaggac accgccgcgc gcgtggatat tccagacagt gggacaaata ccgcgccatg
     7561 tatctgagta aaaacccgtt atgcgtgcga tgccttgaga aggggatata tacgcccgcc
     7621 gtggtggtgg atcacattat cccgattaat ggcggtgatg atgttctctt ctggcccgaa
     7681 tggaaccatc aaccattgtg ccaggcgtgt cataaccaga aaacgaaatg gcttgatccg
     7741 gcaacaaaaa gcaagcgtgc cgcaggtgga tttcgtgaag aggaagaacg ggctgctaac
     7801 cgcaataact ggatgtatga cgctgatgaa tgagcgggaa caaaaccgcc ttatccgtgg
     7861 acttataagg cagcgtgacg catggaagac acaggaaaca gggcataaag ataaagcgtc
     7921 aggacgcgca gaacgcatca cagcgaagcg attaaccgac cgtgaccgcg aggttatgga
     7981 atgtttccgc aatcgctgat gaggctgtat gacggggtgg ggggcgtttt caggacaaac
     8041 ccgaccccgc ctacctggat gatatcgccg tgaagcagtg gcgggaaaaa tcgcggcagc
     8101 ttgcggaaca gggagacctg acccccgccg actggagcaa tctggaactg tattgcgtca
     8161 actactccat ttaccggaaa gccgttgcag accttgcggc gcgcgggttc agcattgtta
     8221 acagtcaggg cggcgagagc agaaaccccg cattaagcgc aaaatccgac gctgaaagag
     8281 tgatgataaa aatggcctcc ttgctcggtt ttgacccgat aagccgccgt aaaaatccac
     8341 cgtaaacaga agaagaggac gagcttgacc gcctggaata agtacgcaga agacgtaaaa
     8401 acgggcaaaa ttccggcctg taaacggtta aaacaggctg ttaaacggta cttttcggac
     8461 cttaaaagcc ccctttacac gttcgatcgt gaggttgtgg agcggtttat tgccttttcc
     8521 agggtgtgcc cgcacgtaaa aggaccgatg cgtggcagac ccattgagct ggagccgtgg
     8581 cagcagttcg cctttgcgtg catcctcggc tttaaggtta aggccaccgg acggcgcaaa
     8641 tacacgagcg cctttatcga agtgccgcgc aaaaatgcga aatccacgac cgccgcgatt
     8701 ctggctaact ggtttctgat tatggagaac ggtcagcagg atatctacac cgcagcggtg
     8761 agccgtgacc aggcgcggat cgtgtttgat aatgcgcgtc agatgtgcct tttatcccga
     8821 ccgttacgca ggcgggtgaa tattcaggcg cataaggtga tacacccgaa aagcaacagc
     8881 ctgttaaagc cgctggcagc aaaagcggca accatcgagg ggacaaaccc cagtctttcc
     8941 atcgtggacg aataccacct acaccccgat aacggcgttt attccgcgct tgagctgggg
     9001 atgggcgcac gtcccgaagg gctgttattt gccattacca catcgggcag taacgtcgtt
     9061 tcagcctgta aacagcacta tgattattgc tgccagatcc tggacggcga agaggtcaac
     9121 gattcaattt ttgtactgat ttacgaactg gacgacgaaa acgaggttga tgatccggcg
     9181 atgtggataa aggcgaaccc taatattgat gtttccgtgg atcgtgaaaa actggcctca
     9241 accatccaga aagcgcgggg tattccgtcg cagtgggtgg agatgatgac aaagcgattt
     9301 aatatctggt gtcaggggac taccccgtgg atgggtaatg gtgcatgggc tgagtgtgcc
     9361 ggaacgttca cggaggaaga tttacacggt caggagtgtt acgcggggct ggacttatca
     9421 tcaaccagcg atatttccag cgtatgctat gcctttccgg tcggtaaaac cattatgctg
     9481 atttcccgtc attatctgcc ggagttccag ctacagaacc ccgccaataa aaaccgcgct
     9541 gtctatcgcc agtgggctaa agcgggctgg atacgcacaa cgccaggtga ctgcattgat
     9601 tacgaccgga tcagagatga catcatgcag gatgcggaga aatttaatat caggctggtg
     9661 ggctttgata cgtggaacgc cacgcatctc aggacgcaat tacagggggc tggttttgag
     9721 gtggagccgt tcccgcaaac ctacctcaga ttcagtccgg cggcgaaatc gttcgaagtt
     9781 tttgttaacc gcagggtgat tgtgcatcgt ggcgatccgg tgttgtcctg gtcgatgagt
     9841 aacgtcgtga tgcagagtga cgcgaacgcc aatatcaagc cgaacaagaa aaaatcaccg
     9901 aacaagatag acccgagcgt agcggcgctg atggcgtttg gtacattcca ggcagagcat
     9961 gaggattttg cttttgatat aagcgacagc caccgccaga aactggaaga atttagcggg
    10021 gtgtaatgag gtcagcagca tgacagaagc cgaaatactg cgattaatcc gccgcgtctc
    10081 tggaatcagc cagcaggctg acgaacagac cacgcagccg gacagcgtga cagccgaaaa
    10141 ttatgcgcgt gttgttgctg aggtgatgcg ccgtgatggt atccagctta atgatgcgga
    10201 tatgcgcgac atacggatcc gcgttctgga aatgctggcc tacaatcgcc gcgttgaact
    10261 gtatcgggag aaggagaaaa tcacgtacca ctggaagaag ccggagcggt tacggcggta
    10321 actcactgag attaaagcaa agcgcaaaat tgcgctggct acatccctca catctgagga
    10381 ctgttaacca gcaggccgga atatattcag aaattacgaa attttcgcag tttaattact
    10441 cgtcagctta atcgctaacc cgctctgata ttttcggaaa cctcaatttg aggaagtcgg
    10501 cgaggtaact gtttgatatt cccgatagcg caaaaatgcg cggtcgggca gaatgaccac
    10561 cacctcaatc gtgacggaga gtgacaacca gtgacacacc gtgacaaaaa gacggtttaa
    10621 ccgttcatta aaaatcgacg tgattttgtg ctttccggat gacgtggcca acatcatttt
    10681 ttagcgaaaa attctgatta aggtcactgg ctgaacggtc ataatgacca cgcagaccat
    10741 cgaggcaacc atagcgatca tcgttacggt tggagctttt ccgccattgg tggaaaaggt
    10801 gcacatctgg atcatcatta cggtgatcaa gggtatgtaa aacccaccag cctgattaaa
    10861 ggttaaccgg aaaaaaaatc cggtagcccc gttatgggga tccccatatc gacattaacg
    10921 cccctcatga attggtgcgc ttccccctgg aaagattacc cgcctgtata tttcttgtgt
    10981 ctatttgttc cacgttgttt catacagtgc accgaacggt gtagttaatg gtgtagtcaa
    11041 tttgcgattt ttggcacttt ttcaatgaaa attgtttgat cgaatcaatg acttgatgta
    11101 gtggtaaaag gacatattta ttcatctttc ggcgcagaat gctggcgacc aaaaatcacc
    11161 tccatccgcg caccgcccag catgctctct ccggcgacga ttttaccctc atattgctcg
    11221 gtgatttcgc gggctaccgc cagccctaca ccttgcccag ggcgtaaagt atcaacccgt
    11281 tgaccacggt cgaaaatgac ctctcgcttg cttaatggaa taccggggcc atcatcctcg
    11341 accacaatat agagatgctc gtcggtttgc cttgcagaaa tttcgacaaa ctcgaggcaa
    11401 tatttacagg cattatccag cacgttgccc atcacctcga caaaatcgtt ctgctcaccg
    11461 acaaagctga tctctggcga aatatcgaga gagatattga cccctttgcg ttgatacact
    11521 ttgttcagcg ctgaggtgag attgtccagc agtggggcga ccggatgcag ctcgcggctg
    11581 agcaatgtcc cgccgcgcat actggcacga tgcaggtagt agccaatttg ctgtgaaatg
    11641 cggctgattt gctccagcat taccggctca gcatcactga cgctcatctt ttcactacgc
    11701 agagaacgca gcgtactttg cagcaccgcc agtggcgttt tcagactatg ggtcaggtcg
    11761 gtgagcgtcg tacggtattt gtcgtaacgt tcgcgttcac tttttaacaa tcggttcagg
    11821 tttcgtacca gactggtcag ttctcgcgtt gtggctggat tgagcaattc gcggttatgt
    11881 tcttccagtt cgcggacttc ttttgccagg gcttcgatgg ggcgtaaact ccaccaggcg
    11941 gcgacccaca gcagcgggat cactaacagc agattggctg agagcacata gataaaccag
    12001 ctccagacca tataggaact ttttagctcc accggaatgg tatccaccac cacaatggtt
    12061 aattttggca tccgcgatgt tgccgggtag acgtttaccg ccaccgagtg ggtcatctcc
    12121 gcgtcgtcat catcttcccg cacttcctgc aactgttgct gtatcgaatg atctccactc
    12181 agcaagaggc tggtatcgtt aacatccgct tcaatttcat gaaaaccatt cgatttcagc
    12241 cagtcaggct ggatcatttt catcagccag ggcacgtcac gttgcgccca taaaagctgc
    12301 ccgttctcat cataaattag cgtcatggtg gggctttgct tgtcgatatt ttcgggtaac
    12361 tcgacatgca acttattgtt ttcccactgc gcaagggtat agaacagatt gctctcgcca
    12421 cgtaacagcc gaaacgtagt tttatcgaaa ctgacgctat aaccgatcag cgcgaccatt
    12481 ccgtaggcaa gcgaaagcac cagtactacc gctgccgttg ccaacagaaa acgtacccgc
    12541 agcgagagcg ggaaaaaaag acgcagtaat tttttcatca gcgcaattcg aacaggtagc
    12601 cctggccgcg aacggtggta atgacttctt gcggatattg tgcctgaatt tttttgcgca
    12661 gacgtcccat cagtacatca atggtatggc tttcccgcag ctccgcatcc ggatagagtt
    12721 gtagcattaa cgaatctttg ctgaccactt tgccattatt gcgtatcaac gtttccataa
    12781 tggtgtattc gaacgcggtc agtttgatca cttcgtcatt aatagataat tcacggcgag
    12841 agagatcaac ctgaaacggg gggagcgaaa tgacctgtga agccagaccg ctattacgcc
    12901 gcattaatgc ctgcattcgc gccatcacct cttcaatatg aaacggttta gtcacgtaat
    12961 catcagcacc ggcacttaat acttcgactt tgtcctgcca gctttcacgg gcggttaata
    13021 ccagaatcgg cagtgaaaca tcgttgctac gccagcggcg aatcagtgac agaccgtcct
    13081 cgtcaggcaa tccgagatcg acaatcgcaa tatccggtaa atgttcattg agataataat
    13141 cggcttcttt ggcatcttct gcgtcatcga cctgatgacc agcatcctga atctgaactt
    13201 taaggtggtg acgtaacaac gcattgtctt caacaaccag tacgcgcatt tttatttctc
    13261 cctgtcttaa ttattaaaat agtgtaacgc gattatgtgt ttatgggggt aaacattaaa
    13321 taaaccagcg gggaggggag gtaaagtgaa aaaagaaaaa gtggataatc ttaataagca
    13381 ggccggacag catcgccatc cggcactgat ttgaggctta tttcagctca tcaaccatcg
    13441 tgatagctcg accaatatag ttagccggcg tcatcgcttt caggcgggct ttctcttctt
    13501 ctggcaacgc cagaccatcg ataaactgct tcatgccttc ggcgtcaacg cgcttaccgc
    13561 gagtaagctc tttcagcttc tcgtacggtt tttcgatgcc atagcgacgc ataactgtct
    13621 ggattggttc tgccagcact tcccagttgt gatccagctc atccagcaga tggtcacggt
    13681 tcacttccag tttgctcacg cctttcaggg tggattgata cgcaatcagc gcataaccga
    13741 tacccacgcc gaggttacgc agcacggtgg agtcggtcag gtcacgctgc cagcgggaaa
    13801 ccggcagttt gcttgccaga tgctgcaata ccgcgttgga aaggcccaga ttcccttcgg
    13861 agttttcgaa gtcgatcggg ttaactttat gcggcatggt ggaagaacca atctcaccag
    13921 caatggtttt ctgtttgaag tggttaaggg cgatataacc ccagacgtca cggtcaaagt
    13981 cgatcagaat ggtgttgaag cgcgcaacgc aatcaaacag ttcggcaatg tagtcgtgcg
    14041 gttcaatctg ggtagtgtac gggttccatt gaatacccag cgaggtgacg aactcttcgc
    14101 tgaactgatg ccagtcaact tccgggtaag cggcgatgtg ggcgttatag ttaccgaccg
    14161 cgccgttgat tttgccgagg atctccacct ggttaagctg gcggtgctgg cgctccatac
    14221 ggtaggcgac gtttgccatc tctttaccga tggttgacgg cgtggctggc tgaccgtggg
    14281 tacgagacag cagcgggata tcgcggtact gaacggcgag atctttaatg ccatcaatca
    14341 gttgacgcca gtacggcagg atcacttcat cacgcgcggt tttcagcatt aatgcgtggg
    14401 agaggttatt gatatcttcc gaagtacagg caaagtggat gaattcagaa accgcgtgca
    14461 gttccgggat ctccgccact ttttctttca ggaaatactc aaccgcttta acgtcgtggt
    14521 tagtggtacg ctcgatggtt ttgatgcgtg cggcatcttc ttcgctgaaa ttggcgacaa
    14581 ttgcatcaag gtaaccgatt gcgtcggcag caaaagcagg aacttccttg atcgctgcgt
    14641 gcgcggccag tttttgcagc caacgtactt caacttgtac acggaatttc agcaaaccat
    14701 attcgctgaa aatcccgcgc agcgcgctga ctttatcgcc gtagcgtcca tcgacagggg
    14761 aaacggcggt cagtgaggat aattccatag atcacaactc cggggttaaa tgagcaagaa
    14821 tttgttttgc ctgagtggtc aggcgattac gagaaaacat cagttgtaga cgtccgccgc
    14881 cgacctggtg ccagagcacg gcggcgcgaa tgcctgccag cagggttgcg cgaactttcg
    14941 cctgcacttg tgggctttgc agtacagcag gggaaccggt gacctgaatg cgcgggccaa
    15001 gcgggctgat cacatcaaca tagatagcag ccatcgcgct catcagcgtt tcggactgta
    15061 aatcgaagtg ttcgagctgg cgttgcaggc cgttgatccg gttgcccaga gtgtcgagcg
    15121 cgcctttcgc tgaggagagt ttgcgctcaa gcaccatcaa gctgagtgtg tagcgggtta
    15181 attcggcgtt taagccctgg caactgctgg cattgagcac gccgagcagg gtttccagcc
    15241 cgacgcgcag gttggcttcg ctaccgccaa aaaccgccag cgtcgagctg gggttcatat
    15301 caataatact gttgagtgag acgtgtagcg catcggcatc acaatgcccc tggtgagcga
    15361 gttgttgcac caggcgtgcc gactgacaaa taccggccag ggcgagggtg atgtcatagt
    15421 aattctttgc cacgttcact gcttccttgt tttaagtaaa gataataatc agaccggcag
    15481 cggcagacgc tgctcaataa taccgccacc gaggcacact tcaccgttat agaagacggc
    15541 agactggccc ggcgtcacgg cggcaaccgg ctcatcgaaa atcactttaa tgcgatcgtc
    15601 gtccagcgcc ttgacggtgc acggaatgtc ggtctggcga tagcgggttt ttaccgtgca
    15661 acgcatagtg ccggtgaatg gttcgcgatc gacccagtgc agctgctggg caatcaaccc
    15721 gacagacatc agccgcgggt gttcatggcc ctgagcgaca accagaatgt tgttttcgac
    15781 gtctttgtcc accacatacc acggttcttc ggtgccttct ttggtgccac cgatacccag
    15841 acctttacgc tgaccgagag tgtgatacat cagcccctgg tgctcaccaa tttcatcgcc
    15901 atcgacggta atgattttgc ccggttgcgc cgggagataa cggcccagga actcgcggaa
    15961 tttacgttcg ccaatgaagc aaatgccggt agagtctttt ttcttcgcgg tgaccagacc
    16021 aagatcttca gcaatcttac gcacctgcgg tttttccagt tcgcctaccg ggaacaggct
    16081 ttgcgcaatc tgctcatggc tgagcgtata aaggaagtag ctctggtctt tattgctgtc
    16141 cagaccacgc agcaggcggc tcttgccatc gacatcggcc cgacgtacgt aatgaccggt
    16201 agcgatataa tcggcaccta aatcttcggc ggcaaattcg aggaaggctt taaatttgat
    16261 ctccttgttg cacagaatat ccggattcgg cgtgcgaccg gctttatatt cggcaaggaa
    16321 cagttcgaag acgttgtccc agtactcggc agcaaagtta acggtgtgca gttcaatgcc
    16381 gagcttgtcg cagaccgcct gggcatcagc cagatccgct gccgctgtgc aatattcctc
    16441 accgtcgtct tcttcccagt tcttcataaa caggccttcg acctgatatc cctgttgttg
    16501 caacagccag gcagaaacgg aggaatcgac accgccggac atgccgacga ttactttttt
    16561 tgcggtttca gacattggat cactcacgac attgaacttc aaggcggcgt attctatcac
    16621 gcagcctttt agatgacacc ctttgtaaaa ggccagttaa aatcgccaat catctccagc
    16681 ggataacgtt gcccgctttg ataacaacga atactttccg ccaccagcgg cgaacgaaga
    16741 tttgacgcct gtaaaatttc ttcggcgctg acccaacggc agcagtcgat atcgctgtca
    16801 tgaggctggg tcgggcatat ttgctcaagc tcaatggcaa agaggaaacg caaaaacggc
    16861 gttttatctg gcgcaatcca ctgatgcata cgaataaagt gttgcggctg cgcgctgatg
    16921 ccggtttctt cccacagttc acgggcggcg gcttccacta aggtttcatc ggcttccaga
    16981 tgcccggcag gttggttcca taacgcttta ccattaatcg tctcttcaac gactaaaaat
    17041 ttgccttctg cgtgcaccac gcaagcaacg gtaacgtgcg gtttaaacat tttgcatcct
    17101 taatctgtca cttctcgcca ttcgccattg gcaagattat ccaaagagta atctcccatc
    17161 gcatagcgaa tcagtcgcag cgtggggaaa ccaacatggg cggtcatgcg gcgcacctgg
    17221 cgattacgtc cttcatataa ggtgatcttc agccagctgg tgggaatact tttgcgttca
    17281 cgaattggtg gattccgcgg ccataaccac gcgggttcgt caaccagctc cgcgcctgcg
    17341 ggcagggtag ggccatcatt taaggttacg ccattgcgca aggcttcaag tgcgtcttgt
    17401 gtgggaatac cttccacctg cacataatag atttttccgg tgcgtttacc cggctgggtt
    17461 aaacgcgcct gcaacgcgcc gttattggtc agcaccagca acccttcgct atcgcggtca
    17521 aggcgacctg ctgcataaac gccctgaacc gggatgaatt cttttaatgt tttgcgtccg
    17581 gtttcatcgg tgaactgcgg aagaacatcg tagggtttat tgaagaggat cacacgcgtg
    17641 ggctggtttt catgtttacg cctggtagaa cgttgtgagc tgaatcgctt aacctggtga
    17701 tttctaaaag aagttttttg catggtattt tcagagatta tgaattgccg cattatagcc
    17761 taataacgcg catctttcat gacggcaaac aatagggtag tattgacaag tcaattacaa
    17821 atcattaaca aaaaattgct ctaaagcatc cgtatcgcag gacgcaaacg catatgcaac
    17881 gtggtggcag acgagcaaac cagtagcgct cgaaggagag gtgaatggaa agtaaagtag
    17941 ttgttccggc acaaggcaag aagatcaccc tgcaaaacgg caaactcaac gttcctgaaa
    18001 atccgattat cccttacatt gaaggtgatg gaatcggtgt agatgtaacc ccagccatgc
    18061 tgaaagtggt cgacgctgca gtcgagaaag cctataaagg cgagcgtaaa atctcctgga
    18121 tggaaattta caccggtgaa aaatccacac aggtttatgg tcaggatgtc tggctgcctg
    18181 ctgaaaccct tgatctgatt cgtgaatatc gcgttgccat taaaggcccg ctgaccactc
    18241 cggttggtgg cggtattcgt tctctcaacg ttgccctgcg tcaggaactg gatctctaca
    18301 tctgcctgcg tccggtacgt tactatcagg gcactccaag cccggttaaa caccctgaac
    18361 tgaccgatat ggttatcttc cgtgaaaact cggaagacat ttatgcgggt atcgaatgga
    18421 aagctgactc tgccgacgcc gagaaagtga ttaaattcct gcgtgaagag atgggcgtga
    18481 agaaaattcg cttcccggaa cattgcggta tcggtattaa gccgtgttct gaagaaggca
    18541 ccaaacgtct ggttcgtgca gcgatcgaat acgcaattgc taacgatcgt gactctgtga
    18601 ccctggtgca caaaggcaac atcatgaagt tcaccgaagg cgcgtttaaa gactggggct
    18661 accagctggc gcgtgaagaa tttggcggtg aactgatcga cggcggcccg tggctgaaag
    18721 ttaaaaaccc gaataccggc aaagagatcg tcattaaaga cgtgattgct gatgcattcc
    18781 tgcaacagat cctgctgcgt ccggctgaat atgatgttat cgcctgtatg aacctgaacg
    18841 gtgactacat ttctgacgct ctggcagcgc aggttggcgg tatcggtatc gcccctggag
    18901 caaacatcgg tgacgaatgc gccctgtttg aagccaccca cggtactgcg ccgaaatacg
    18961 ccggtcagga caaagtaaac cctggctcta ttattctctc cgctgagatg atgttacgcc
    19021 atatgggctg gactgaagcc gcagacctga ttgttaaagg tatggaaggc gcgatcaacg
    19081 ccaagaccgt aacctatgac ttcgagcgtc tgatggaagg cgctaaactg ctgaaatgtt
    19141 cagagtttgg tgatgcgatc atcaagaata tgtaatcact acatgtgtta aatatttaaa
    19201 cgggcgtata acacgcccgt tgttttattt atgtggatat tattaatagc atatcgagca
    19261 tat
//
LOCUS       DAEEGA010000080        17506 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEEGA010000080 DAEEGA010000000
VERSION     DAEEGA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17506
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.080"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(163..894)
                     /gene="rlmB"
                     /locus_tag="KLH46_003002"
     CDS             complement(163..894)
                     /gene="rlmB"
                     /locus_tag="KLH46_003002"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBE0379604.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1074..3515)
                     /gene="rnr"
                     /locus_tag="KLH46_003003"
     CDS             complement(1074..3515)
                     /gene="rnr"
                     /locus_tag="KLH46_003003"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBE0379605.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3554..3979)
                     /gene="nsrR"
                     /locus_tag="KLH46_003004"
     CDS             complement(3554..3979)
                     /gene="nsrR"
                     /locus_tag="KLH46_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBE0379606.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4184..5482)
                     /gene="purA"
                     /locus_tag="KLH46_003005"
     CDS             complement(4184..5482)
                     /gene="purA"
                     /locus_tag="KLH46_003005"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBE0379607.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5586..5783)
                     /locus_tag="KLH46_003006"
     CDS             complement(5586..5783)
                     /locus_tag="KLH46_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBE0379608.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5865..6869)
                     /gene="hflC"
                     /locus_tag="KLH46_003007"
     CDS             complement(5865..6869)
                     /gene="hflC"
                     /locus_tag="KLH46_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBE0379609.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6872..8131)
                     /gene="hflK"
                     /locus_tag="KLH46_003008"
     CDS             complement(6872..8131)
                     /gene="hflK"
                     /locus_tag="KLH46_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBE0379610.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8217..9497)
                     /gene="hflX"
                     /locus_tag="KLH46_003009"
     CDS             complement(8217..9497)
                     /gene="hflX"
                     /locus_tag="KLH46_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBE0379611.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9573..9881)
                     /gene="hfq"
                     /locus_tag="KLH46_003010"
     CDS             complement(9573..9881)
                     /gene="hfq"
                     /locus_tag="KLH46_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBE0379612.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9967..10917)
                     /gene="miaA"
                     /locus_tag="KLH46_003011"
     CDS             complement(9967..10917)
                     /gene="miaA"
                     /locus_tag="KLH46_003011"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBE0379613.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10910..12757)
                     /gene="mutL"
                     /locus_tag="KLH46_003012"
     CDS             complement(10910..12757)
                     /gene="mutL"
                     /locus_tag="KLH46_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBE0379614.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12767..14104)
                     /gene="amiB"
                     /locus_tag="KLH46_003013"
     CDS             complement(12767..14104)
                     /gene="amiB"
                     /locus_tag="KLH46_003013"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBE0379615.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14123..14584)
                     /gene="tsaE"
                     /locus_tag="KLH46_003014"
     CDS             complement(14123..14584)
                     /gene="tsaE"
                     /locus_tag="KLH46_003014"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBE0379616.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14556..16103)
                     /gene="nnr"
                     /locus_tag="KLH46_003015"
     CDS             complement(14556..16103)
                     /gene="nnr"
                     /locus_tag="KLH46_003015"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBE0379617.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16102..17241
                     /gene="queG"
                     /locus_tag="KLH46_003016"
     CDS             16102..17241
                     /gene="queG"
                     /locus_tag="KLH46_003016"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBE0379618.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17224..17277)
                     /locus_tag="KLH46_003017"
     CDS             complement(17224..17277)
                     /locus_tag="KLH46_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379619.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3984 a         4991 c         4393 g         4138 t
ORIGIN      
        1 ccgttacgca ccacgccttc agtagcggag caggaaggac atctgatgga aatggaagcc
       61 acgcaagcac cttaaaatca ccatcataca ctaaatcagt aagttggcag cattaccaaa
      121 aaaaccatcc aaatctggat ggcttttcat aattcagagg aactagctgc gctgacgaac
      181 cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc agggaagaaa cacttcctgc
      241 cattgggatg ctgatcaact catcgcaatg ttcacgggtc aggcgacgca taccttcacc
      301 ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg ctctgataga gtgtatgatc
      361 cgcctcgcct gccgtaccga cgatccagat attctcttcc tgcaacatac gcatggtgcg
      421 cgcaaggtta gtcacccgaa tcagcggaac gctttctgcc gcgccgcagg ctactttttt
      481 cgccgtagcg ttgagctgtg cggagcgatc tttcggcaca atcaccgcat gaacgcctgc
      541 ggcgtccgca ctacgcaggc acgcgccgag gttgtgcgga tcggttacgc cgtcgaggat
      601 cagcaggaac ggttgatcga gcgaagcgat cagatccggc agatcgtttt cctgatactg
      661 acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg gcaccgtcgc ttttctcgtc
      721 gagatattgg cggtttgcca actggataac cacgccctga gactcaaggg cgtgaatcag
      781 cggtaacaga cgtttatctt cacggccttt taaaataaag acttcctgaa aacgttcagg
      841 ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa atcatttcgc tcattaatgt
      901 actcgttgtt tacgttttgc ctgatgcact acgtttatcc ggcctacatg atctctgcaa
      961 tatattgaat ttgcgtgatt atgtaggccg gataaggcgt tcacgccgca tccggcatga
     1021 acaaagcgca ttttgtcagc aatctaaccc tcttttttta aagagggtat tgatcactct
     1081 gccacttttt tcttcgccgc acgcttcgct ttggtcgctg cggctatttt ctgcgttttc
     1141 gccgatggct ttttcgcttt tctcgcgtct ttcttcgccg ctttcggctt cgtttttttc
     1201 tcaccgcgga aggcgctgtc tggctcaaag tttacctttt taccgacctg acgacgcttg
     1261 ccgccttttt tgcctgcatc gccttttttc gctttctcgc gcgccgtttt accgacgttg
     1321 cgcggtgcgc gttcactgga gatcagacta aagtcgattt tgcgctcgtc catattaacc
     1381 gcttcgacgc gaacttccac gcgatcgccc aggcgataag tctggccgct ggattccccc
     1441 atcaggcgtt gccctacctg gtcaaagcga tagtagtcat tgtccagcga agagacatgg
     1501 accagaccat caatgaacaa gtcgtccaga cggacgaaga agccaaagcc agtgacgctg
     1561 gaaattacgc ctttaaagac gttacctacc tggtcgagca tgaagtcaca cttcagccag
     1621 tcagcgacat cgcgcgttgc ttcgtcggca cgacgttccg ccatcgaaca gtgctgaccc
     1681 agttgcaaca tctcttccat cgaataatgg tagccgccgg tttcagtggt gttgccctga
     1741 tgcccctgct ctttcgccag cagatattta atggcgcggt gcagcgtcag gtcaggataa
     1801 cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg ccagaccgaa gtgaccacgg
     1861 ttttctggat cgtaaatcgc ctgtttcatc gagcgtagca gcatggtttg cagcatttct
     1921 gcatcaggac ggtcggcaac cgactccagc agttccgcgt agtcacgcgg ttccggcttg
     1981 ttaccacccg gcagctccag ccccagctcc gccagcactg aacggaaaga ggtaatcgct
     2041 tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt ctttcgcttt ctcaacgaaa
     2101 cgcgccgccg agatattcgc cagaatcatg cactcttcaa ttaatttgtg cgcgtcgtta
     2161 cgctgggtct gttcgatacg ttcaatacgg cgttcagcgt tgaaaatgaa cttcgcttct
     2221 tcgctctcaa atgagatccc accgcgttct tcacgggctt tatccagcac tttatagagg
     2281 ttatgcaact cttcgagatg cttaaccagc ggggcgtact gctcgcgcag atcctgatcg
     2341 ccctgcagaa tatgccagac tttggtgtag gtcagacgcg cgtgagagct catcaccgct
     2401 tcgtagaatt tgtagcccgt caggcggcct ttcgacgaaa ccgtcatctc gcacaccata
     2461 cacaggcggt ctacctgcgg gttgagcgaa cacaggccgt tagagagcac ttccggcagc
     2521 atcgggataa cctgcgaagg gaagtacacc gacgtgccac ggttacgcgc ttctctgtcc
     2581 agcggcgttg gcggacgcac atagtagctg acgtcggcaa tcgcgaccca taaacgccag
     2641 ccgccgccgc gttttttctc gcagtaaact gcatcgtcaa agtcacgggc gtcttcgcca
     2701 tcaatggtga ccagcggtaa atcgcgcaaa tcgacacggc ccgcttttgc ttcttccggc
     2761 acttcttctt tcagccccgc aacctgttgc tcaacagcct gcggccagat atacggaatt
     2821 tcatgggtac gcagagcgat atcaaccgcc atgccggtgc ccatattgtc gcccagcact
     2881 tcgacgattt tacccaccgc tttggtgcgg cgagtcggac gctgagtcag ttcgactacg
     2941 accacaaagc ccatccgcgc gcccatgatc tgatcgggcg ggattaagat atcgaagctc
     3001 agacggctat cgtcaggaac cacaaagccg acgcccgctt cggtaaagta gcgaccaaca
     3061 atctggctgg tttttggcac cagtacgcgg acaatacgcg cttcacgacg acctttacgg
     3121 tcagcaccca gcggctgcgc cagcacctga tcgccatgaa tgcaggtttt catctgctcg
     3181 ctggagagat acaaatcatc tttacgccct tcaacccgca gaaagccgta gccatcacgg
     3241 tgaccaataa cggtaccttt caccaggtcg aggcgttccg gcagcgcata gcactgacga
     3301 cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca ggcggcgacg caggccttca
     3361 agctgctctt cgccttcaat gtgcagttct accgccagct catcacggct ggccggtttt
     3421 tcacgtttgg ttaaatgttc gaggataaat tcccgactag ggatgggatt cgcgtatttt
     3481 tcagcttcgc gttcctggaa aggatcttgt gacatctcgg ttcctccgtt gtcatctctg
     3541 atgaagattt tcgtcactcc accagcaata atttataaag cggttgattc tcttcaacca
     3601 aatcggcaag cgtgtagtta tccagttccg taagaaaact ttgcacggcc ttagaaagcg
     3661 cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc actgctgcaa ttcaccagcg
     3721 ataagggctc cagctcgcgc accacatcac caatacgtat cgcactcgcc ggtttaccca
     3781 ggcgaatgcc gccatttttt ccacgaacag cagtcacgta gccggcacga ctaagttgat
     3841 tgattatttt gaccatatga ttacgggaga cgccgtagac gtcagtcact tcagaaatac
     3901 tggtcatccg cccttctggc aatgacgcca tgtagatcag cgcacgtaat ccgtaatcag
     3961 tgaaactcgt taactgcaca tcaacctcaa aagggaaatc gggaaaaaat acatttatat
     4021 tgatgataaa ccagccacaa gctgtgtcgc taatttattt cagttcgggg aaggaaaaaa
     4081 gcgaggattt aacactgtgt cggatagcgt aatggcttaa ccgacacagc aatgtatcac
     4141 actgttagcc cggcaggcaa aatatctgcc aggcgtacca gaattacgcg tcgaacgggt
     4201 cgcgcagaat catggtttca gtacgatccg gaccggtaga gatgatatcg atcggcacac
     4261 cggtcagctc ttcaatacgc ttgatgtagt tcagcgccgc ctgcggcagg ccgctacgat
     4321 ctttcacgcc gaaagtggat tcagaccagc ccggcatggt ttcgtaaatc ggctctacac
     4381 ctttccagtc gtcagctgcc agcggagtgg tagtcacttc gcgaccatcc ggcatacggt
     4441 aagccacgca gagtttaacc tctttcaggc catccagaac gtccagttta gtcaggcaga
     4501 agccagacag ggagttcagc tgtaccgcac gacgaacggc aacggtgtcc agccagccgg
     4561 tacgacgacg acgacccgta gttgcaccga attcgttacc ctgcttgcag aggaactcgc
     4621 cagtttcatc aaacagttcg gccgggaaag gacctgcacc tacacgagtt gagtaagctt
     4681 tgaggatacc cagaacgtaa tcaacataac gcgggcccag gccggaaccg gtcgccacgc
     4741 caccagcagt ggtgttggaa gaagttacgt acggataagt accgtggtcg atatccagca
     4801 gcgtaccctg cgcaccttcg aacatgacga aatcgccacg ctgacgcgcc tggtcgagca
     4861 ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc aacagccatc gtatcatcca
     4921 gaactttctg gtaatcaacc gcttcagctt tgtagtagtt aaccaactgg aagttgtgat
     4981 attccatcac ttctttcagt ttttcagcga aggtttcttt gtcgaaaagg tcgccaacac
     5041 gcagaccgcg acgtgccact ttatcttcat aagcaggccc gataccacga ccggtggtgc
     5101 cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc cagagcaacg tgataatcaa
     5161 ggatcagcgg acatgcttca gacagcagca gacgctcacg aacggggatg ccacggtctt
     5221 ccagttcttt catctctttc atcagcgcgg ccggagacag cacaacaccg ttaccgatga
     5281 tgctggttac attctcgcgg agaatacctg atggaataag atggagaacg gttttttcac
     5341 cgttgattac gagagtatgg cctgcgttgt gaccgccctg gtagcgtaca acatatttag
     5401 cccgttcagt cagaagatcg acgatcttac ctttaccttc gtcaccccat tgggtgccca
     5461 gtacgacgac gttgttaccc atttttcaaa atcaccgttt gcttaaaaat ggattctacc
     5521 atcgcttttt cagagttaca gcactttttg cactcaaaaa tgacctcgat cagtctgttt
     5581 tttgttcagc caatcgtttt cctcaacatg tagtagacca caacgcccgc aaccacaagt
     5641 ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg tcatcgcaga gatcatcttc
     5701 ttccatgcct tcgggtaaag catcggccct aaaccttcca gtaccaaaac cagggcaagc
     5761 gccagccaga ttgtcgaatt cattcattat ccttatagaa aaagaaaacc accgacatcc
     5821 ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt tatattaacg cgttgcggaa
     5881 gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc tcatgaccat cacgtcctga
     5941 ttgccagaga agctgttctc ataagcacgc aggctacgga tgaatgcgta gaagtccgga
     6001 tctttactga atgcatcagc aaacagtttg gctgcttcgg catcgccttc accacgcata
     6061 atgcggccct gacgctcagc ttctgccagc gttctggtca cttcatagtc ggcagtcgcg
     6121 cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac gacgcgctac cgcttcacgc
     6181 tcggcgcgca tacggttgta gatcgcttca gacacttcgg tcggcaggtt gatctgcttg
     6241 atacgcacat cgacaacttc aatacccagc gccgccatac tgttcgggtt gatgaccgga
     6301 actttgccct tcgtctcagc cgttacgcgc tctgccgctt cggcaatggc gttatctgct
     6361 gccggggtag taacttcatc ttctgtaccc gcagaaccgg agttcagcgc gtcacgtact
     6421 tcgagggtca gacgaccacg ggaatcggtg acgatatctt tcacgtccag gcgaccaatt
     6481 tcagaacgca gacggtcaga gaacttacgt ttcaacagca cttccgcttg cgaaatgtcg
     6541 ccaccacccg ttgccaggta gtaacggctg aaatcgctga tgcgccattt gatgtaagag
     6601 tcgacgatca ggtctttctt ctctttggtc acaaagcggt cggcctggtt gtccatggtc
     6661 tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg gtatcttgaa atgcagacca
     6721 ggctcataaa ccaaaggttt gttgtcatcg tcacgcagta ccttaccaaa acgcagcgta
     6781 ataccgcgct caccttcttt gacgacaaag acagacatgt aaagcactac cagcacgatg
     6841 atgataatcg cgataactga cttacgcatc gttattcccc ctgacgctgg tagtcgttac
     6901 gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg actggtggac gacgtgttgc
     6961 ttgctccact ggttgtggag gaagaggctg gcggcagacg cagcagattg ctggcaccgt
     7021 tatcgctctt cgccgcaggg gcgttaccac ctttcagcat ctggtctaac ggcagaacca
     7081 tcaggttgcc acctttatcg ttaaccagca ctttgcgggt gttacccaac actttttcca
     7141 tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc ggctttatat tccggcagaa
     7201 gtttagcaaa gcgcgccact tcaccctgag cttccaggat ggtctgggcc ttgtacgcac
     7261 gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg cggctgaact tcgttggtat
     7321 acgcttctgc ttcacgaatg tattgctgtt cgttttcacg cgcggcaatc gcatcgtcaa
     7381 acgccgcttt tacttcttcc ggcggacgag cagcctggaa gttgacgtcc agcagcgtga
     7441 tacccatgtc atacggacga atcgtctctt ccagttcgcg ctgagtatcg ctacgaatca
     7501 cggtacgacc ttccgtcaga atgcggtcca tggtgtattt accgataact ccacgcaggg
     7561 cgctgtcggt agcctgacgc aggctgtcat ccgggctggt cacgctatac agatattttt
     7621 ccggattggt gacgcggtac tgcacgttca tctcaacgcg cactacgttc tcgtccgacg
     7681 tcagcatcac accagaagcg gccagttcac gcacggcttc cacgttcacc ggtttgactt
     7741 cgtcgataaa cgtcggtttc cagttcagac ccggctcaac cagatggctg aatttaccaa
     7801 agcgtgttac cacgccgcgt tcggcttctt taatggtata gaaaccactg gccgcccaga
     7861 taatgacaat cgctgccgct gcgatggtaa cgacacgacc gccaagctgc gggcgcgggc
     7921 cttgcgatga actgccaccg ccagatccgg tgcctttacc gccgcccaga ccaccgagct
     7981 ttttgctcag tttgcggaag atatcatcta aatcaggtgg cccttgatcg cgaccgcctt
     8041 tgtttccatt tccctcagag ttgccgccag gtttgctgct tccccacggg tcgcggtctt
     8101 gtccgttatt accgggctga ttccacgcca tgtttgtgct ccatatttgt tatgcggtga
     8161 tccctgttgt ctttcgacgc cagaggatat gactccacgc ttcagacgct acgccgttag
     8221 atcaggtaat cgatcaacgc cggttcttgt ttacagaggc gacgccagtc aacgatcggc
     8281 atacgaactt gcagacttac gctgccgtcc tcctccatcc actctttttc tattgcctga
     8341 agctgataaa aacgactcct cagacgccct tcctgcggtg gcagacgcaa tgtatgctgc
     8401 gccacctcgc cggaaagccg ctccgtcaaa gcctgaaaaa gctgtggtat ccccgctccg
     8461 gtctgtgcgg aaagccagac acggatcggt ttgttctctt cgtcccgatc aatacgcggt
     8521 tcgaaatctt ccagcatatc gatcttgttc atcaccagca gggttgggat ctcgtgagcg
     8581 tcgatctctt caagaaccgt attcaccgct tcgatgtttt cttgtacacg cacatccgcc
     8641 gcatcaatga cgtgcagcag taatgtggct tgccgcgtct cttgtaacgt ggctttaaat
     8701 gccgccacca gatcgtgcgg caggtggcga ataaacccta cggtatctgc aagtacggtt
     8761 tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt cgagggtggc aaacaactgg
     8821 tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa gggtagattt accggcgttg
     8881 gtatatccca ccagcgaaac agtaggaacg tcggctttga tacgcgattg ccgcccctgc
     8941 tcacgctgct tttcaactct ttccaggcgc gactgtatct gcacgatgcg attacgcaac
     9001 aaacgacggt cggtttcgag ctgggtttca cccggaccac gcaaacctat cccgcctttc
     9061 tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag ccagatggcg cagctgcgcc
     9121 agctcaacct gcaacttacc ctcatgggta cgcgcacgtt gggcgaaaat atctaaaata
     9181 aggccggtgc ggtcgataac acgacactcg cacaaacgct ccaggttacg ctcttgcgcc
     9241 gggctcaggg catggtcaaa aagaacgacc gaagcaccca tcgctttgac agcttccgca
     9301 atttcaactg ctttaccttc acctacaaaa tactttgggt gcggcgcttt acggctaccg
     9361 gtaatcacct gcaatgcttc gacaccggcg gaagagacca gagattcaaa ctcctggagg
     9421 tcttccatat ctttgtcttg cgtaaaatag atgtgtacca gtaccgcctg ctcaccagca
     9481 tcataacggt caaacaagcg tataaccctc taaatagatc agcggggaac gcaggatcgc
     9541 tggctccccg tgtaaaaaaa cagcccgaaa ccttattcgg tttcttcgct gtcctgttgc
     9601 gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc gccaccggcg
     9661 ttgttactgt gatgagaaac cgggcgagat gggacaacag tagaaatcgc gtgcttgtaa
     9721 accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga ctcgatttgc
     9781 ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc ccgacgcagt
     9841 gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt tccttatatg
     9901 cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg tctcaattgt
     9961 acacattcag cctgcgatag caccaacaac ctgtaatact tcgtcacgcg cctgttctgg
    10021 tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta tctgccgctt
    10081 cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg agatttcgcc
    10141 ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca agtccgtatg
    10201 caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag ccaacatctg
    10261 atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg cgatggcgaa
    10321 ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg ttaaagtttt
    10381 acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat ttggatgaat
    10441 ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact cccaaccttg
    10501 ctctgccgcc tgttgctcaa ttctggcccg cacttccggg tctgccgacg gtagcggcga
    10561 caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca gtgggatccg
    10621 ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat cagcagccga
    10681 gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga gtaactcttc
    10741 agcgttcggc ttcgccgtcc cgataaccat ccctttgtaa ataagggcag aatcaacgct
    10801 tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt taccggaggc
    10861 cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat cactcatctt
    10921 tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc ggcggcgttt
    10981 tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc tgtgccattg
    11041 accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata ttgccaggtt
    11101 cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt tgtaaatttt
    11161 gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct gactggaaat
    11221 caatacccaa ttccgctagg gcagactgcg ctttttctaa tgccgatttt tcttcgccag
    11281 aaacttttag ccgcaacgga atcagcagcg gctgggcgca aacgggcgct tcacccggcg
    11341 tcaattgtgc ctgacgcagc caacgttctg ccactggcaa ggataaaagt gaaatgttgc
    11401 catcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga ccaaaactct
    11461 gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc ttttggtttt tgcatcggcg
    11521 cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag cctggctgcg
    11581 cattcggcca gggggcagcc ggacgactgc ctgaggcggg cgcaggagag tagcgcggag
    11641 ctaccggctc acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc acgcggtttt
    11701 ccgggatggc acgcggtgcg ggttgtggtt catcgtccag cggtagcggc gtttccagtt
    11761 gctgttgtag cacgctcagc acgccctgat agataaaatc atgcaccaga cgcgactgat
    11821 ggaaacgcac ttcgtgtttg gcggggtgta cgttgacgtc cacctgatgc gggtcgatct
    11881 ccagatacaa cacaaatgcc ggttgctgat cggcccccag tttgtcttcg caggcctggc
    11941 ggatcgcatg attgatcagg cgatcgcgca tcatgcgacc attcacgtag caatactgaa
    12001 tttccgccag tgcgggcgta gtgtgatttg gatcggccac ccagccgcgc agggtgagat
    12061 cgccgtgttg ccattcaatc gccagcgctt gttcgagaaa agcggtgccg caaatcgcgc
    12121 ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc acaattttac
    12181 cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg cggatgattt
    12241 catcaatatg gctaaattcg gttttctcgg tgcgcaggaa tttgcgccgc gccggggtgt
    12301 tgtagaacag atccagcacc tccagcgtcg ttcccaccgg atgcgctgcc ggtttaaccg
    12361 tcacgtccat gtcgcgtcct tcggcatagg cctgccaggc ttcctgctgt tctgcggtgc
    12421 gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca ccgcgaaagc
    12481 ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg gcatgacgag
    12541 ccagcgccag cgccagctca tcttttttga taccgcagcc gttatcacga atgcggataa
    12601 gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca tcgaggctgt
    12661 tttccactag ttctttgact accgacgcag gtcgctcgac cacctcacct gcggcaatct
    12721 ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag tttggcagcg
    12781 tgcgatctgg cgtcgtcacc gtactggcag tttgtgccgt tgcaccctgc ggcgcagatt
    12841 gcatcggatg cgcaaggaag taattgcgca ggcctttgta aatggcttct gccagctgtt
    12901 gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata aaaccggttt
    12961 cgaccagtac tgatgggata tccggcgagc gcagaacgcc aaggctggcg tgttctggtc
    13021 gacgtttatg tatttcgcca atgcgttgca actgactgat catactggtc gctacatcat
    13081 accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt aaataggggt
    13141 cagactgact gttcgccagc acatcacccg ccccacccag cagctccgac agtttctcgt
    13201 actgctccag ccagctggcc atttcactgt tggcgcgacg gttagagagc acccatacgg
    13261 aagcgccagt cgcactacgg ttcggtgcgg catcagcgtg aatcgacacg aggaaattgg
    13321 cgttttgctt atgtgccaca tcactgcgcc ccatcaccga gataaagtaa tccccgtcac
    13381 gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtacgcaat ttacgcgcga
    13441 tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca gggtcctgac
    13501 cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc gtcgcgcgcg
    13561 ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta ctttccgttt
    13621 taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc gtttcaacgc
    13681 gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac atcggcgtta atcgtaaaga
    13741 cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttaccg ttttcggtaa
    13801 gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga atcgccttca
    13861 ccagattatt gccgctgaac aacaacggca gtccctgaat cacgcccgtt tgtttgatat
    13921 cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg ccaataaaac
    13981 tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag agcgtcgcgg
    14041 cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa ccaatttctg atgcgataca
    14101 tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat tcacccgcag
    14161 aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg tgtatttcga
    14221 catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg cagatggcat
    14281 cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg gcaaggcggt
    14341 acaaatcaaa gtgatagacc attaagttgt cgagcgtata gggttcgacc agcgtataag
    14401 tggggctttt gacattaccc tgatgaccca gagcctgtaa aaagccccgg ctaaaggtgg
    14461 ttttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg caggctttcg
    14521 ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt actcgattca
    14581 tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga aaagagatcg
    14641 gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc cgcaccgtgc
    14701 gcgacacagc ctgcacaggc tgcatcatac ggcgacagtt tttgcccaag caatgcgcca
    14761 ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt tccggcatca
    14821 ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt cagcaccgct
    14881 acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact ttcaatttca
    14941 gcgacggaac agcctaacaa ccgtgcggcc tcgctaggat gcggcgtgat cacgcgattg
    15001 tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca caacatcggt
    15061 ttgcgaaaat tctcaacttt ttgcagggct tttttccccc actcttgctg gcccagaccg
    15121 ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc catagtcagt
    15181 tcatgcacca tcaattctgg tcgtgcagtc agcagcggcg caatgttctc actgcgggtc
    15241 agtactcgga ctaaaccagc accagcacgt agcgccgctt cccccgtcat acgaatagcc
    15301 cccgccgtgc cgtgatcgcc accgataatc accagccgcc cgtgatcgcc tttatgcgaa
    15361 gtcgggcgac gcggtttcag ccagtgagaa agttgttctg ccgaaaaccg ctgaattttc
    15421 gtctcctgac ctgccagcca gctatccagc cccagtgagt caaaatgcag ttgtcctgta
    15481 acatcccgcg cttttccagt gagcaagcct agtttcagcg caataaaagt gatggtgtga
    15541 tcggcgttga tcactgcgcc tggcgtagcg ccggtttcag ccagcaggcc ggaagggata
    15601 tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg gctaatggat
    15661 tcgcggggcg cttgctgcaa gccggtaccg agcagcgcat caacaatcag atctaccgat
    15721 tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca tgcttcgcgt
    15781 gccagcgcgg cctcttccgg caacggtttg tcgctctcct gggccaacaa cgtgacctca
    15841 atgccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt attaccatga
    15901 ccgcacagca ccagccagtg gcgggcgtca ggatacgccg aacgacacac ctggaatgcg
    15961 gcctcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcacatc tgccgcctcg
    16021 cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact tacggggttt
    16081 ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt agcgcaaaaa
    16141 attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga taccgatctc
    16201 agcgagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg cgaaatggac
    16261 tggatggcac gtcacggtat gctgcgcgct cgcccccatg agttattacc cggtacgctg
    16321 cgtgtaatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc cagcacgctg
    16381 aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta tcacaaactt
    16441 ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt ttcgctgaat
    16501 tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga aaaagctggg
    16561 ctcggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc gttcttcttt
    16621 ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga ggaaggatgc
    16681 ggcaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga gccatatacc
    16741 gtcgatgctc gccgctgtat ctcttatctc accatcgaac ttgaaggggc gctcccggaa
    16801 gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca gcttatctgc
    16861 ccgtggaatc gctattcgca actcactaca gaagacgatt tcagcccgcg taagccgcta
    16921 cacgcaccgg aactcattga gttatttgcc tggagcgaag agaagttttt aaaagtcacg
    16981 gaaggatcgg cgattcgccg tataggtcac ctgcgctggc tgcgtaatat cgccgtggca
    17041 ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc tggaaagtcg taaaggtgag
    17101 cacccacttc tcgatgagca catagcgtgg gcgattgcgc agcaaataga gagacgaaat
    17161 gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt gattgaaaaa
    17221 gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa aacgcattgc
    17281 aattcaagac gaataaaatc gtcaagtgat cgactcgaca aaacggaatc ttgattttta
    17341 acttatttat caataggtta aaaaatacca ttcaccgtgc gaagtttctt tgaaattaag
    17401 attacagcct gaggctgtgg ataactctgt ttacaagagt ttgtcagaga caacaaaaac
    17461 cagcaataac gcatcgcgtc gctgtagata ttttattgag agaaga
//
LOCUS       DAEEGA010000081        17173 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEEGA010000081 DAEEGA010000000
VERSION     DAEEGA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17173
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.081"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..185
                     /locus_tag="KLH46_003018"
     CDS             <1..185
                     /locus_tag="KLH46_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBE0379620.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="KLH46_003019"
     CDS             complement(210..401)
                     /locus_tag="KLH46_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBE0379621.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="KLH46_003020"
     CDS             complement(506..742)
                     /locus_tag="KLH46_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBE0379622.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="KLH46_003021"
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="KLH46_003021"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBE0379623.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="KLH46_003022"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="KLH46_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="KLH46_003023"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="KLH46_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBE0379624.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="KLH46_003024"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="KLH46_003024"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBE0379625.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="KLH46_003025"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="KLH46_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBE0379626.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="KLH46_003026"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="KLH46_003026"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBE0379627.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="KLH46_003027"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="KLH46_003027"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBE0379628.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="KLH46_003028"
     CDS             complement(10860..12233)
                     /locus_tag="KLH46_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0379629.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="KLH46_003029"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="KLH46_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBE0379630.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="KLH46_003030"
     CDS             complement(13344..13583)
                     /locus_tag="KLH46_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379631.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="KLH46_003031"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="KLH46_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBE0379632.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="KLH46_003032"
     CDS             13960..14247
                     /locus_tag="KLH46_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBE0379633.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14826..14969
                     /locus_tag="KLH46_003033"
     CDS             14826..14969
                     /locus_tag="KLH46_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBE0379634.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14982..15191
                     /gene="cspE"
                     /locus_tag="KLH46_003034"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14982..15191
                     /gene="cspE"
                     /locus_tag="KLH46_003034"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBE0379635.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15357..16166
                     /gene="rlmA"
                     /locus_tag="KLH46_003035"
     CDS             15357..16166
                     /gene="rlmA"
                     /locus_tag="KLH46_003035"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBE0379636.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="KLH46_003036"
     CDS             complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="KLH46_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBE0379637.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIITTALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4532 a         4155 c         4337 g         4149 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggtcgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaaccgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcta ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctccgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtac
     1561 aggttgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtcttt gcactcaaac tcacggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac cgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgatgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagcgg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttatta gcgaaacgtt tctctcttga
    14401 tttttttgct cactcatcaa tttttcttat tttaaattta caatcccttt ggggattgac
    14461 ttctctttag ggtaattgat agccgttaac tgactgtttt atgagaaaaa gtgatataac
    14521 tttttattca ttgcatagca gaaaatgtga tattgcacgc actatgtaat aacttctccc
    14581 tctggcctgg aacaactgaa cttattgaac tatgttagaa actacgccag tttaagtatc
    14641 tgcctgaact ggcaaggtta agcacaatga tatatcggcg cgtattccgt tgcataagtg
    14701 tgcaaaaaaa gtggaagacg tatcgagatt tgtgcgtctg atcgagacat gtttaaaaat
    14761 ggcttgccat aattaacgtt gtatgtgata aaagatttcg ggttaaacga ggtacagttc
    14821 tgtttatgtg tggcattttc agtaaagaag tcctgagtaa acacgttgac gttgaatacc
    14881 gcttctctgc cgagccttat attggtgcct catgcagtaa tgtgtcagtt ttatctatgt
    14941 tatgcctgcg ggcgaagaaa acaatctaag gaatttttca aatggcaaag attaaaggtc
    15001 aggttaagtg gttcaacgag tctaaaggtt ttggcttcat tactccggct gatggcagca
    15061 aagatgtgtt cgtacacttc tccgctatcc agggtaatgg cttcaaaact ctggctgaag
    15121 gtcagaacgt tgagttcgaa attcaggacg gccagaaagg tccggcagct gttaacgtaa
    15181 cagctatctg atcgaatcca ctgatctgaa gtgtgaatac gcttcaatct cgctataaag
    15241 cctcgtcgaa tgcgaggctt tttactatgc tttatcttcg ctcctggcgt tcggatattt
    15301 gcccgccgcg tgattcgcgt tacacttgcg gcctttagta tcctgctgga gttgtcatgt
    15361 cgttttcctg tccactttgc catcagcctc tttcgcgtga aaaaaacagc tatatctgtc
    15421 cccaacgaca tcagtttgat atggcgaaag aagggtatgt caatctgctg cccgttcagc
    15481 ataaacggtc tcgtgatccg ggcgacagcg cggaaatgat gcaagcacgc cgcgcattct
    15541 tagatgccgg acattatcag ccgctgcgtg atgcaattgt cgcccaactg cgggaacggc
    15601 ttgatgaaaa ggccacggcg gtgctggata ttggctgtgg tgaagggtat tacacacacg
    15661 catttgccga tgcgttgccc gaaatcacca cgtttggcct ggatgtctcg aaggtagcga
    15721 taaaagcggc ggcgaaacgc tatccgcagg tcactctttg tgtcgcttcc agccaccgtt
    15781 tgccgttttc cgataccagt atggacgcca taatacgtat ttacgcgccg tgtaaagcag
    15841 aagaattagc acgagtagtg aagcccggcg gctgggtcat tactgccacg ccgggaccgc
    15901 gacatttgat ggagctgaag gggctgattt acaatgaagt acatcttcat gcacctcatg
    15961 cagaacaact ggaaggtttt acattacagc agagtgcgga gttgtgttat ccgatgcgtc
    16021 ttcgcggtga tgaagccgtc gcattattgc agatgacgcc gtttgcctgg cgtgcgaagc
    16081 cagaagtctg gcaaacactg gcagcaaaag aagtgttcga ctgccagacg gactttaata
    16141 ttcacctctg gcagcgttct tattaaccgt ggaagtgcgt ccagaggatc tggacgccga
    16201 tgccgatcag caccagcccg ccgagaattt ccgctttttt cccaataatt gagccgataa
    16261 agcgaccaac catcatccct aatgtcgaca taatcaaggt tgcacaacca atggccaatg
    16321 cggtcgtgat aatgttgacc tgaaggaaag caagaccaac acccacagcc atggcatcca
    16381 ggctggtggc aatcgcggtg gttaccagta gccagaaacc gtgccgacgg cgcggctctt
    16441 catcttcatc atctgcgcca cgaaaaccct caataatcat tcgcccgccg aggaatatca
    16501 gcagcacaaa cgcaatccag tgattccatt caaggacaaa ccggctggct aacatgccca
    16561 ttccccagcc gatcagcggc gtcagggttt cgacggcacc aaaaataagg ccggttcgca
    16621 atgcttcaga aaatttcggt ttatggaggg gggcaccttt accgattgat gcagcaaatg
    16681 catccatcga cataccaaac gcaagaagaa cagtagcagt gatattcatg acaacgtcct
    16741 gaccggggta tccataaaac acatcatcgc ccccagcaaa catgcataca tgaacaacaa
    16801 gccgcgatgg gttttcgcgt tcatgcaggc atagcgattg atgtgcatat ggtctcgcct
    16861 gactgaaagt cttcagtccg tacgtgccac gttttgcaac gagtatgttg acacgtacat
    16921 ttccggaatc tggaaatcgg ctactcccca aatgagggcg caaccttaac atattttgag
    16981 aatataaaac aactgttgga atatattatt tcattgcttt attgataacg attttcattt
    17041 atgtttatat atcaatgaaa cgttaaaata aataacaaat aagggaagag ataatatagc
    17101 ctaagctatg tctgattatt aaaaatacac taaacatagc tttggctaaa ttcataagtt
    17161 attgagttga tac
//
LOCUS       DAEEGA010000082        17144 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEEGA010000082 DAEEGA010000000
VERSION     DAEEGA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17144
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.082"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(52..306)
                     /locus_tag="KLH46_003037"
     CDS             complement(52..306)
                     /locus_tag="KLH46_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HBE0379638.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPVTLFVPLIMGC"
     gene            616..2388
                     /gene="aspS"
                     /locus_tag="KLH46_003038"
     CDS             616..2388
                     /gene="aspS"
                     /locus_tag="KLH46_003038"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBE0379639.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2506..2958
                     /gene="nudB"
                     /locus_tag="KLH46_003039"
     CDS             2506..2958
                     /gene="nudB"
                     /locus_tag="KLH46_003039"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBE0379640.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2987..3727
                     /locus_tag="KLH46_003040"
     CDS             2987..3727
                     /locus_tag="KLH46_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE0379641.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3762..4283
                     /gene="ruvC"
                     /locus_tag="KLH46_003041"
     CDS             3762..4283
                     /gene="ruvC"
                     /locus_tag="KLH46_003041"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBE0379642.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4285..4887)
                     /locus_tag="KLH46_003042"
     CDS             complement(4285..4887)
                     /locus_tag="KLH46_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBE0379643.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4956..5021
                     /gene="yobI"
                     /locus_tag="KLH46_003043"
     CDS             4956..5021
                     /gene="yobI"
                     /locus_tag="KLH46_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HBE0379644.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5160..5771
                     /gene="ruvA"
                     /locus_tag="KLH46_003044"
     CDS             5160..5771
                     /gene="ruvA"
                     /locus_tag="KLH46_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBE0379645.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5780..6790
                     /gene="ruvB"
                     /locus_tag="KLH46_003045"
     CDS             5780..6790
                     /gene="ruvB"
                     /locus_tag="KLH46_003045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBE0379646.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6937..7722)
                     /gene="znuB"
                     /locus_tag="KLH46_003046"
     CDS             complement(6937..7722)
                     /gene="znuB"
                     /locus_tag="KLH46_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBE0379647.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7719..8474)
                     /gene="znuC"
                     /locus_tag="KLH46_003047"
     CDS             complement(7719..8474)
                     /gene="znuC"
                     /locus_tag="KLH46_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBE0379648.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8553..9485
                     /gene="znuA"
                     /locus_tag="KLH46_003048"
     CDS             8553..9485
                     /gene="znuA"
                     /locus_tag="KLH46_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBE0379649.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9501..10823
                     /gene="mepM"
                     /locus_tag="KLH46_003049"
     CDS             9501..10823
                     /gene="mepM"
                     /locus_tag="KLH46_003049"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBE0379650.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10943..11914
                     /gene="lpxM"
                     /locus_tag="KLH46_003050"
                     /gene_synonym="msbB"
     CDS             10943..11914
                     /gene="lpxM"
                     /locus_tag="KLH46_003050"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBE0379651.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(12045..13487)
                     /gene="pyk"
                     /locus_tag="KLH46_003051"
     CDS             complement(12045..13487)
                     /gene="pyk"
                     /locus_tag="KLH46_003051"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBE0379652.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13615..14484)
                     /gene="hexR"
                     /locus_tag="KLH46_003052"
                     /gene_synonym="yebK"
     CDS             complement(13615..14484)
                     /gene="hexR"
                     /locus_tag="KLH46_003052"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBE0379653.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14821..16296
                     /gene="zwf"
                     /locus_tag="KLH46_003053"
     CDS             14821..16296
                     /gene="zwf"
                     /locus_tag="KLH46_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBE0379654.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16531..>17144
                     /locus_tag="KLH46_003054"
     CDS             16531..>17144
                     /locus_tag="KLH46_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBE0379655.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDR"
BASE COUNT         4375 a         4229 c         4463 g         4077 t
ORIGIN      
        1 ccccccggat catggacgac tgggggcagt aatcatgccg ggtctggctg gctaacaccc
       61 cattataagg ggtacgaata aagtaacggg ggcatcaacc ggctgtttta atgcttcggc
      121 gtaatcggca gaccagccaa ctcgcaccag aaacacgggc tgaccgctgg cgcgaaattt
      181 cgccgccagc ttcccggcgc gattaactac ctcatcggca gtatgtggac caccggcaaa
      241 aggtaagatg ccttcttgta aatcgatcac caccaacgca gtggttttag cattaagttc
      301 aagcatgctg actcctgaaa aaacaaaatc gtgcctcaca ccttaatgcg attagccgat
      361 ggcggaacga caaattttgt taatttttat gagttcgggc aacaccttta tagcaaactg
      421 gcgtagagtc tgatttcagg cccgatataa agtggtaacg aggcgttcca gcgcgggcca
      481 ggctcttacg gtgcggcaga atttccagta taatagccgc cttttttcat tcagttgtga
      541 catacagcta acgctgcgac ttggtcacct gcgcagcagg gggcatccgc cggcagctaa
      601 gttaagggat atctcatgcg tacagaatat tgtggacagc tccgtttgtc ccacgtgggg
      661 cagcaggtga ctctgtgtgg ttgggtcaac cgtcgtcgtg atcttggtag cctgatcttc
      721 atcgatatgc gcgaccgcga aggtatcgtg caggtatttt tcgatccgga tcgtgcggac
      781 gcgttaaagc tggcctctga actgcgtaat gagttctgca ttcaggttac gggcaccgta
      841 cgtgcgcgtg acgaaaaaaa tattaaccgc gatatggcga ccggcgaaat tgaagtgctg
      901 gcgtcctcgc tgactatcat caaccgcgca gatgttctgc cgcttgactc taaccacgtc
      961 aacaccgaag aagcgcgtct gaaataccgc tacctcgacc tgcgtcgccc ggaaatggcg
     1021 cagcgcctga aaacccgcgc taaaatcacc agcctggtgc gccgttttat ggatgaccac
     1081 ggcttcctcg acatcgaaac tccgatgctg accaaagcca cgccggaagg cgcgcgtgac
     1141 tacctggtgc cttctcgtgt gcacaaaggt aaattctacg cactgccgca atccccacag
     1201 ttgttcaaac agctgctgat gatgtccggt tttgaccgtt actatcagat cgttaaatgc
     1261 ttccgtgacg aagacctgcg cgctgatcgt cagcctgaat ttactcagat cgatgtggaa
     1321 acttctttca tgaccgcgcc gcaagtgcgt gaagtgatgg aagcgctggt gcgtcatttg
     1381 tggctggaag tgaagggcgt ggatctgggc gatttcccgg taatgacctt tgcggaagca
     1441 gaacgccgtt atggttctga taaaccggat ctgcgtaacc cgatggaact gaccgacgtt
     1501 gccgatctgc tgaaatctgt tgagtttgca gtatttgcgg ggccggcgaa cgatccgaaa
     1561 ggccgtgttg ccgctctacg tgttccgggt ggcgcatcgc tgacccgtaa gcagatcgac
     1621 gaatacggta acttcgttaa aatctacggc gcgaaaggtc tggcttacat caaagttaac
     1681 gaacgcgcga aaggtctgga aggtatcaac agcccggtag cgaagttcct taatgcagaa
     1741 atcatcgaag ccatcctgga tcgtactgcc gcgcaagatg gcgatatgat tttcttcggt
     1801 gccgacaaca agaaaattgt tgccgacggg atgggcgcac tgctcctgaa agtgggtaaa
     1861 gaccttggtc tgaccgacga aagcaaatgg gcaccgctgt gggttatcga cttcccgatg
     1921 tttgaagacg acggtgaagg cggcctgacg gcaatgcacc atccgttcac ctcaccgaaa
     1981 gatatgactg ctgcagaact gaaagctgca ccggaaaatg cggtggcgaa cgcttacgat
     2041 atggtcatca atggttacga agtgggcggt ggttcagtac gtatccataa tggtgatatg
     2101 cagcagacgg tgtttggtat tctgggtatc aacgaagagg aacagcgcga gaaattcggc
     2161 ttcctgctcg acgccctgaa atacggtact ccgccgcacg caggtctggc attcggtctt
     2221 gaccgtctga ccatgctgct gactggcacc gacaatatcc gtgacgttat cgccttcccg
     2281 aaaaccacgg cggcagcgtg tctgatgact gaagcaccga gctttgctaa cccgactgca
     2341 ctggctgagc tgagcattca ggttgtgaag aaggctgaga ataactgata tgactcaaat
     2401 acacgaaatc attcgcgttg catcaaggcg gcaactgagt gaactcccat gagcatagat
     2461 aactatgtga atgggatgag cgaaggcagt caacgaagag gcagcgtgaa ggataaagtg
     2521 tataagcgtc ccgtttcgat cttagtggtc atctatgcac aagatacgaa acgggtgctg
     2581 atgttgcagc ggcgtgacga tcccgatttc tggcagtcgg taaccggcag cgtggaagag
     2641 ggtgaaaccg cgccgcaagc tgccatgcgc gaagtaaagg aagaggttac cattgatgtt
     2701 gtcgctgaac aactgacctt aattgactgt cagcgcacgg tagagtttga aattttttca
     2761 catttacgtc atcgctatgc gccgggcgtg acgcgtaata cggaatcatg gttctgtctt
     2821 gcgcttccgc acgagcggca gatcgttttc actgaacatc tggcttacaa gtggcttgat
     2881 gcgcctgctg cggcggcgct cactaagtcc tggagcaacc ggcaggcgat tgaacagttt
     2941 gtaattaacg ctgcctgaac aggcagggct atctggagat atttttatgg caggtcatag
     3001 taaatgggcc aacaccagac atcgtaaagc tgcgcaggat gctaagcgcg gtaaaatctt
     3061 cactaaaatc attcgtgagc tggtaaccgc ggctaagctg ggcggtggcg atccggacgc
     3121 taacccgcgt ctgcgtgcgg cggtggataa agcactgtct aacaacatga cccgtgacac
     3181 actgaaccgc gcaattgcac gtggtgtggg cggtgatgat gatgcaaaca tggaaaccat
     3241 catctacgaa ggttacggtc ctggcggcac ggcaatcatg attgaatgtc tgtctgacaa
     3301 ccgcaaccgt accgttgctg aagtgcgtca cgcattcagc aaatgtggcg gtaacctcgg
     3361 cactgacggt tccgtagctt atctgttcag caaaaaaggc gtgatctcct tcgagaaagg
     3421 cgacgaagac accatcatgg aagcagctct ggaagcaggt gctgaagacg ttgtgacata
     3481 tgatgacggc gcgattgatg tctacactgc atgggaagaa atgggtaaag tgcgcgacgc
     3541 tctggaagcg gcaggtctga aagcagacag cgcggaagtt tccatgatcc cgtccaccaa
     3601 agctgatatg gatgcagaaa ccgcgccgaa actgatgcgt ctgatcgata tgctggaaga
     3661 ttgcgacgac gtgcaggaag tttaccataa cggtgaaatc tctgatgagg ttgcagcgac
     3721 tctctgatga tgcctgctaa acagcaaaac ggagacgcgt gatggctatt attctcggca
     3781 ttgacccggg ttcgcgcgtg accggctacg gcgtcatccg ccaggtaggt aggcaactgt
     3841 cctacctggg tagcggatgc atacgcacca aagtggatga tttaccgtct cgtctgaagc
     3901 tcatctatgc gggcgtgacg gaaatcatca cccagttcca gcctgattat ttcgccattg
     3961 aacaagtctt tatggcgaag aacgctgact cagccctgaa actgggccag gcgcgcggcg
     4021 tggcgattgt tgcggcggtg aatcaggaat tgccggtatt tgaatacgcg gcacgtcagg
     4081 taaagcaaac ggtggtaggt atgggtagtg ccgaaaaaag ccaggtgcag catatggtcc
     4141 gcaccttgct gaaactgccc gctaatccac aggcggatgc cgccgatgcg ctggcgattg
     4201 ccatcaccca ctgccacgtt agtcagaatg cgatgcagat gagcgaatcg cggctgaacc
     4261 tgacgagagg gcgactgcgt taagttatac cgcctcggtc agttccggct gaggcgtttc
     4321 aactccctcc gcgtgaatca acaccaaacc cggatgatgc cacaaactgg ctggcgtgac
     4381 ggttttacgc tcccacggaa tagaacctaa gaaccagaat ttccagaaag tgagttttgc
     4441 attcggattg ctatttaaca gttgtccaaa cgtctctatt tcacccaccg gaatacatag
     4501 cgcctcttta taaatatcaa agacaaattt tgaacagaac tggcgcgaag attcgtattt
     4561 aaaacccgtg tggtaaagtt tgcgtagccg ggagggaacc tgttcaacaa ttcgttgttt
     4621 ttgttgttct gtcagcccag cgtctaatcg ctttatagca tagcgttgat tagacgaacg
     4681 tttaataaaa cgagatagcg tggtgatggt tgaaagggga acccggcttt ctgcaaccag
     4741 gaagtcttca ccgttatgac cgataatgat cccgacgtga ttactccagc aatttgatgc
     4801 agctgatatt tgaccaaata aggcagcacc tatacatgta aagacgatat ctccaatttc
     4861 atattcagca gggtaattaa tattcacgtt gtcattccat tgaaatagat acgaatatac
     4921 gtaatagcat aaaaaattat ttttcaggag tcgcaatgta tatttttatt acgcatttct
     4981 tcactgaata tgtaatattc aaatatttgc ttccaatata acctgttgaa taaattatac
     5041 tgtgccattt ttcagatcat cgggacacct cgcaagtttt cttcatcctt cgctggatat
     5101 ctatccagca tttttttatc atacagcatt atctttgatt cattacgcag gagcgtcatg
     5161 tgataggcag actcagaggc atcatcattg aaaaacaacc cccgctggtg ttaattgaag
     5221 tgggcggcgt aggctatgaa gtgcatatgc cgatgacctg tttttatgaa ctccctgaag
     5281 cgggtcagga agcgatcgtt ttcacccact ttgtggtgcg tgaagacgcg caactgctgt
     5341 acggttttaa caataaacaa gaacgcacgt tgttcaaaga gttgatcaaa accaacggcg
     5401 tcggcccgaa gttggccctg gcgatcctct ccggaatgtc agcgcagcag ttcgttaatg
     5461 ccgttgagcg tgaagaagtg ggggcactgg tgaaactgcc gggtattggc aaaaaaaccg
     5521 ccgaacgctt gattgttgaa atgaaagacc gatttaaagg tttgcatggc gatctcttta
     5581 cgccagccgc cgacctggta ctcacgtctc ctgctagccc ggcgaccgac gatgctgaac
     5641 aagaagcagt tgccgcgctg gtggcgctag gctataaacc acaagaagca agccgcatgg
     5701 tgagcaaaat cgctcgccct gacaccagca gtgaaacatt aattcgcgaa gccctacgcg
     5761 ccgcgttatg aggtaaagga tgattgaagc agaccgtctg atttctgccg gtaccacttt
     5821 gccggaagat gtagcagatc gcgccattcg ccctaaatta ctggaagagt atgttggtca
     5881 gccgcaggtt cgttcacaga tggagatttt catcaaagca gcgaaactgc gcggcgatgc
     5941 cctcgatcat ttgttgattt ttggtcctcc ggggttgggt aaaactacgc ttgccaacat
     6001 tgtcgccaat gaaatgggcg ttaatttacg cacgacttct ggtccggtgc tggaaaaggc
     6061 gggcgatttg gctgcgatgc tcactaacct tgaaccgcat gacgtgctgt ttattgatga
     6121 gatccaccgt ttatcgccag ttgttgaaga agtgctgtac ccggcaatgg aagactacca
     6181 actggatatc atgattggtg aaggtccggc ggcacgctcc attaaaattg atttgccgcc
     6241 gtttaccctg attggcgcaa ccacgcgcgc aggttcgctg acatcaccgt tgcgcgaccg
     6301 ttttggtatt gtgcaacgtc tggagtttta tcaggtgccg gatctgcaat atatcgtcag
     6361 tcgcagtgca cgctttatgg ggcttgagat gagtgatgac ggtgcgcagg aagtagctcg
     6421 tcgcgctcgc ggtacgccac gtattgccaa ccgtctgctg cgtcgagtgc gtgatttcgc
     6481 cgaagtgaag cacgatggca ccatctcggc agatatcgct gctcaggcgc tggatatgtt
     6541 gaatgtcgat gctgaaggtt tcgattatat ggaccgcaaa ttgttgctgg cggtaatcga
     6601 taagttcttt ggtgggccgg taggtctgga taacctggcg gcagccattg gcgaagaacg
     6661 tgaaaccatt gaagatgtac tggaacctta tttgattcag caaggctttt tgcagcgtac
     6721 accgcgcggg cgtatggcga cgacgcgggc gtggaatcac tttggcataa cgccgccaga
     6781 aatgccgtaa gtcggattgg tcgctcgcgc cgcatccgac actggcagtg tgtctgatgc
     6841 gacactgttc gcgtcttatc aggccagcca gagatagcga accgtaggtc ggataaggcg
     6901 ctcgcgccgc atccgacaaa tgtgttcagc gatagattag ctggcctgct ttttcatcat
     6961 actgataata aataacagtg ccgcacatag caccaccgac ggacccgccg gcgtatcgta
     7021 aaacgcggaa aaggttaaac cgccagttac tgccaccatc cccaccaaaa cagcgacacc
     7081 agccatctgt tccggcgtac gggcaaagcg acgcgcagta gctgcaggaa taatcagcaa
     7141 cgaagtaata atcaacgcac cgacaaattt catcgctaca ccaatcgtca atgccgtcac
     7201 cagcatcaac aacaatttca cttgctgtaa tttcacacca tcaacaaacg ccagatccgg
     7261 gctaatcgtc atcgacagca aattgcgcca ttgccagaac aaaatagcca ccacgatgac
     7321 cacgccaatc gcaatagaga tgagatcttc tggcgtcact gccagtaaat caccgaacag
     7381 gtaagccatc aaatcaacac gaatgttaga catcagacta acgaccacta ggcccagcga
     7441 cagggcactg tgcgccataa tccctaataa cgtgtcgatc gccagctgtg gacgcttctc
     7501 cagccatacc agaccgcccg ccagcagcag cgtaacggca atcaccgcat agaatggatt
     7561 cacgtccagc aacaaaccaa acgcgacacc aagtaatgag gcatgagcca gcgtatcacc
     7621 gaaataagac atacgacgcc agactacaaa cgaacccagc ggacccgcgg cacaggcgag
     7681 catgatcccg gctaaccaac cgggaaataa taattcaatc atgagcgatc atttccccga
     7741 cgcaaaacaa ttcgtccctg taaatcgtga cgatgattat gatgatggcg atagataccc
     7801 agttgttcag caccacgagg accaaacatt gaaataaact ccggatgcag ggaaacaact
     7861 tccggtgtgc cggaacaaca aatgtggtga ttcaggcaca gcacttcatc ggtttttgcc
     7921 atcaccagat gcagatcgtg agaaaccatt aaaacgccac aatccagttc acgacgcaac
     7981 tggtcaataa ggtcatataa cgccacttgg ccatttacat ccacgccttg agtgggttca
     8041 tccagcacta ataattgcgg gcaatttaac aatgctcgcg ctaatagtac acgctgcgtt
     8101 tcgccaccag agagcttttg catcggtgcg ttaatcaggt gcccggcctg gacacgtttc
     8161 agtgcaggca aaatatcttc tttatgtgta ccagggcgta agcgtaaaaa acggtttacg
     8221 gtcagtggca acgtggtgtc gagatacagc ttctgcggta catagccgat gcgcagtttt
     8281 ccgttgcgct tgataacccc ttcatcgggt gttaccagcc cgagcactac ccgtaccagt
     8341 gtcgacttac ctgcgccatt tggcccaagt aaagtcaaaa tttttccagg tttaagttcc
     8401 agcgacacat cagagaggac gcggcgttgg ccaaaagaaa ccgagacatt ttccagggaa
     8461 accagacttg tcatgttaat tttagtcttg cagtagtcat gaaatgttat aatatcacac
     8521 ttctcatatt cattacgatg attggtcgca ttatgttaca taaaaaaacg cttcttttcg
     8581 cagcattatc cgccgctctc tggggaggtg caacacaggc cgcagatgcc gccgttgtcg
     8641 cttcgcttaa acccgttggg ttcatcgctt ctgccattgc tgatggtgta acagaaacgg
     8701 aggttttact gcctgacggc gcttcagaac atgattattc actgcgtcca tcggatgtaa
     8761 aacgcttaca gaacgcggac ttagtcgttt gggttggccc ggagatggaa gcgtttatgc
     8821 aaaaaccggt aagcaaatta ccagaagcga agcaggtaac gattgcgcag cttgaagatg
     8881 tgaaaccgct actgatgaaa agtattcacg gcgatgatga tgatcacgac cacgcggaaa
     8941 aaagtgacga agatcaccat cacggcgatt tcaacatgca tctttggctt tccccagaga
     9001 tagcgcgggc tacagcggtt gcaatccatg gaaaattagt ggaacttatg ccgcaaagtc
     9061 gagccaaact tgacgccaac ctgaaggatt ttgaggcaca attggcctca accgaaacgc
     9121 aggttggtaa cgagctcgcg ccgctcaagg ggaaaggtta tttcgttttt cacgatgctt
     9181 acggctattt cgaaaaacag ttcggactga caccgcttgg tcattttacc gttaaccctg
     9241 agattcaacc tggcgcgcag cgtttacatg aaataagaac acagttggtt gagcaaaaag
     9301 caacctgcgt ttttgctgag ccacagttca ggccagcggt cgttgagagc gtcgcacgag
     9361 ggacatccgt tcgtatggga acgttggatc ccctggggac gaatatcaaa ctgggtaaaa
     9421 caagctattc agaattcctg agtcaattag ccaaccagta tgcgagctgc ctgaaaggag
     9481 attaatgagg aagtgattac gtgcaacaga tagcccgctc tgtcgccttg gcgtttaata
     9541 atttaccgcg accacaccgc gttatgttgg ggtcgctcac cgttcttact ctggccgtcg
     9601 ctgtctggcg gccctatgtt tatcaccgtg atgccacgcc aattgtcaaa accattgagc
     9661 tggaacagaa cgaaattcgt tcgctcttac ctgaagccag tgagccgatt gatcaagctg
     9721 cacaagaaga tgaagccatt ccccaggacg aactggatga caaaatcgcc ggtgaagcgg
     9781 gcgtgcatga atatgttgtt tccactggcg atacgctaag cagcattctc aatcagtatg
     9841 gtattgatat gggtgatatc acccaacttg ctgctgccga caaagaattg cgtaacctga
     9901 aaatcggtca acaactctcc tggacattaa ccgcggacgg cgaactgcag cgcctcacct
     9961 gggaagtgtc tcgtcgtgaa acccgaacct atgaccgtac tgccgctaac ggttttaaaa
    10021 tgaccagcga aatgcagcaa ggagagtggg ttaacaatct gctgaaaggt accgtcggag
    10081 ggagctttgt tgccagcgcc agaaacgccg gtttaaccag cgccgaagtg agcgcagtga
    10141 ttaaagccat gcagtggcaa atggatttcc gcaaactgaa aaaaggcgat gaatttgcgg
    10201 tattaatgtc tcgagaaatg cttgatggta aacgtgagca aagccagctg ctgggcgtac
    10261 gtttgcgttc agaaggtaaa gattattacg caatccgcgc tgaggatggc aaattctacg
    10321 atcgtaacgg tactggtctg gcgaaaggat tcttgcgatt cccgacggcg aaacagttcc
    10381 gtatctcgtc taactttaac ccgcgtcgaa ctaatccggt gacaggtcgc gttgcaccac
    10441 acagaggtgt tgatttcgcc atgccacaag gtacgccagt gctttcagtg ggtgacggtg
    10501 aagtggtggt tgccaaacgc agtggcgcag caggttatta tgtggctatt cgtcatggtc
    10561 gcagctacac cacgcgttat atgcacttgc gcaagattct ggtgaaaccg ggacagaagg
    10621 tgaaacgtgg cgaccgtatc gcgctttccg gtaataccgg acgttcaact gggccgcatc
    10681 tgcactatga agtatggata aaccagcagg ccgtaaaccc gctgacggca aaactgccgc
    10741 gtaccgaagg gctgaccggc tccgatcgtc gcgaattcct ggcgcaggcc aaagagattg
    10801 tgccgcagct acggtttgat taattaacat ccattcgcag ccggtacgca gtcagtaccg
    10861 gcttttttta tttggtgcgg ggcaagttgt gccgctacac tatcaccaga ttgatttttg
    10921 ccttatccga aactggaaaa gcatggaaac gaaaaaaaat aatagcgaat acattcctga
    10981 gtttgataaa tcctttcgcc acccgcgcta ctggggagca tggctgggtg tagcagcgat
    11041 ggcgggtatc gctttaacgc cgccaaagtt ccgtgatccc attctggcac ggctgggacg
    11101 ttttgccgga cgactgggaa aaagctcacg ccgtcgtgcg ttaatcaatc tgtcgctctg
    11161 ctttccagaa cgtagtgaag ctgaacgcga agcgattgtt gatgagatgt ttgccaccgc
    11221 gccgcaagcg atggcaatga tggctgagtt ggcaatacgc gggccggaga aaattcagcc
    11281 gcgcgttggc tggcaagggc tggagatcat cgaagagatg cggcgtaata acgagaaagt
    11341 tatctttctg gtgccgcacg gttgggccgt cgatattcct gccatgctga tggcctcgca
    11401 agggcagaaa atggcagcga tgttccataa tcagggcaac ccggtttttg attatgtctg
    11461 gaacacggtg cgtcgtcgct ttggcggtcg tctgcatgcg agaaatgacg gtattaaacc
    11521 attcatccag tcggtacgtc aggggtactg gggatattat ttacccgatc aggatcatgg
    11581 cccagagcac agcgaatttg tggatttctt tgccacctat aaagcgacgt tgcccgcgat
    11641 tggtcgtttg atgaaagtat gccgtgcgcg cgttgtaccg ctgtttccga tttatgatgg
    11701 caagacgcat cgtctgacga ttcaggtgcg cccaccgatg gatgatctgt tagaggcgga
    11761 tgatcatacg attgagcggc ggatgaatga agaagtcgag atttttgttg gtccgcgacc
    11821 agaacaatac acctggatac taaaattgct gaaaactcgc aaaccgggcg aaatccagcc
    11881 gtataagcgc aaagatcttt atcccatcaa ataaaaaagc ctctcgcgag gagaggcctt
    11941 cgcctgatga taagttcaag tttgcttcag aatattcgaa atctgttgaa ctatcattga
    12001 actataggcc ggatgtggcg ttttcgccgc atccggcaac gtacttactc taccgttaaa
    12061 atacgcgtgg tattagtaga acccacggta ctcatcacgt cgccttgggt gacaatcacc
    12121 aggtcaccag acatcaagta acctttatcg cgcagcagat taaccgcttc gctggcagct
    12181 gctacgccgt cattagcgct atcaaagtgc accggcgtaa cgccacgata gagagcagtc
    12241 aggttcagcg tacgttcatg gcgcgacatg gcgaaaattg gtagaccaga gctgatacgg
    12301 gaggtcatca gcgcggtacg acccgattcg gtcatggtaa tgatcgccgt aacgcccttc
    12361 aggtggttag ctgcgtacat tgccgacatg gcaatagctt cttccacatt gtcgaactga
    12421 acgtccagac ggtgtttaga aacgttgatg ctcgggattt tttccgcacc caggcaaacg
    12481 cgcgccatgg ctgcaacagt ttctgacgga tactgcccag cggcagtttc tgcagacagc
    12541 atcacagcgt cagtaccatc cagaacggcg tttgctacgt ccatgacttc tgcacgcgtc
    12601 ggcatcgggt tagtaatcat tgactccatc atctgggtcg ccgtgattac cgctcggttt
    12661 agctgacgcg cacgacggat caacgctttc tgaatgccga ccagttccgg gtcgccaatt
    12721 tccacaccga ggtcgccacg tgcaaccatt accacgtcag aggcgaggat gatgtcatcc
    12781 attgcatcct ggctgcaaac ggcttccgca cgttcaacct tggcaacaat tttcgcatca
    12841 catcctgcat cgcgtgccag gcgacgggca tagttcagat cttcgccaca gcgtgggaag
    12901 gagacagcca ggtaatctac gccaatcaac gccgcagtct taatgtctgc tttgtctttt
    12961 tcggtcagcg cttcagccga caaaccgccg ccaagtttgt tgataccttt attgttggag
    13021 aggggaccac cgacggtgac ttcggtgaac actttcatgc cctgaacttc cagtactttt
    13081 aactggacgc gaccatcgtc cagcagcagg atgtcaccag gcacgacgtc agcaggcagg
    13141 cctttgtagt cgataccgac tttttctttg tcgccttcac ctttacccag gttggcgtcg
    13201 agcaggaatt tatccccaat attgaggaaa actttgcctt ctttaaaggt ggatacacgg
    13261 attttgggcc cctggaggtc acccagaata gccacatgac gccccagttt tgcggcaatc
    13321 tcacgaactt tatccgcgcg cattttgtga tcttcaggcg agccgtgaga aaagttcata
    13381 cgtacaacgt tggcacccgc cgcgataact ttttcaagat tattatcgcg atctgttgct
    13441 gggcctaacg tggtaacgat ttttgttctg cgaagccttt tggacatgta atactccgtt
    13501 gactgaaaca accaggtgtt gcttgaacat gaaatccgaa tgaaataacg ccgcgatggc
    13561 gataaattaa tctcattcgg atgtcgtata agattaggac agtgacagtc gcttttagcg
    13621 atcgtcactt aaattaagta actgcttatc aaaacgcgat tccttcagcg cttccttgac
    13681 ccgcttcaag ttatctctga attttgcacc gcgtcgcaaa gtaaatcctg tcgccagcac
    13741 atctatcacg gtcagctgtg caagtcgaga aaccatgggc atataaatgt cagtatcttc
    13801 cggtacgtcg agggtaattg ccagcgttgc ttcccgggcg agcggggtac ccgcagaggt
    13861 gagggcaatc accatggcgt cgttttcgcg tgccagctgc gccagctcga ccagattttt
    13921 tgttcttcct gtgtgagaaa tcagcaccac cacgtctccg tcgctacaat tcatacaact
    13981 catgcgttgc agcacgatat catcggagta caccaccgga acattaaaac gaaagaactt
    14041 attcatcgca tcgtgggcaa cggcggcaga agagcctaat ccgaaaaagg cgattttttt
    14101 tgcctgagtg agcaagtcga cggcgcggtt gatggcagat ttatccagtg aatgacggac
    14161 atgatcgagc gttgccattg cggactcaaa tattttcccc gtgtatgatt caacgctgtc
    14221 atcttcattg acgttgcgat taacataggg agtgccattc gccagacttt gtgccagatg
    14281 aagtttaaaa tcaggaaaac cgcgcgtgtc catgctgcga cagaaacgat tcaccgtcgg
    14341 ttcgctaaca ttggcttcca gcgccagagc agcaatactc gaatggatcg cgttatcggg
    14401 cgaagccaga atgacctcgg caactttgcg ctctgatttg ctcaaatgtt ccagctgaga
    14461 ctggattttt tccagcatat tcatgatgta aagagactca cgggtaatga cgatttccgc
    14521 actgaaagaa atcgaaatgc agttttgtca gatattacgc ctgtgtgccg tgttaatgac
    14581 aaaagcagat aaaaaagttg ttattttttt cataacatga tcagtgtcag atttttaccg
    14641 aatggaaaac gatgattttt ttatcagttt tgccgcactt tgcgcgcttt tcccgtaatc
    14701 gcacgggtgg ataagcgttt acagttttcg caagctcgta aaagcagtac agtgcaccgt
    14761 aagaaaatta caagtatacc ctggcttaag taccgggtta gttaacttaa ggagaatgac
    14821 atggcggtaa cgcaaacagc ccaggcctgt gacctggtca ttttcggcgc gaaaggcgac
    14881 cttgcgcgtc gtaaattgct gccttccctg tatcaactgg aaaaagtcgg tcagctcaac
    14941 ccggacaccc ggattatcgg cgtagggcgt gctgactggg ataaagcggc atataccaaa
    15001 gttgtccgcg aggcgctcga aactttcatg aaagaaacca ttgatgaagg tttatgggac
    15061 accctgagtg cacgtctgga tttttgtaat ctcgatgtca atgacactgc tgcattcaac
    15121 cgtctcggcg cgatgctgga tcaaaaaaat cgtatcacta ttaactactt tgccatgccg
    15181 cccagcactt ttggcgcaat ttgcaaaggg cttggcgagg caaaactgaa tgctaaaccg
    15241 gcacgcgtag tcatggagaa accgctgggg acgtcgctgg cgacctcgca ggaaatcaat
    15301 gatcaggttg gcgaatactt cgaggagtgc caggtttacc gtatcgacca ctatcttggt
    15361 aaagaaacgg tgctgaacct gttggcgctg cgttttgcta actccctgtt tgtgaataac
    15421 tgggacaatc gcaccattga tcatgttgag attaccgtgg cagaagaagt ggggatcgaa
    15481 gggcgctggg gctattttga taaagccggt cagatgcgtg acatgattca gaaccacctg
    15541 ctacaaattc tctgcatgat tgcgatgtct ccgccatctg acctgagcgc agacagcatc
    15601 cgcgatgaaa aagtgaaagt actgaagtcc ctgcgccgca tcgaccgctc caacgtacgc
    15661 gaaaaaaccg tacgcgggca atatactgcg ggcttcgccc agggcaaaaa agtgccggga
    15721 tatctggaag aagagggcgc gaacaaaagc agcaatacag aaaccttcgt ggcgatccgc
    15781 gtcgacattg ataactggcg ctgggccggt gtgccattct acctgcgtac tggtaaacgt
    15841 ctgccgacca aatgttctga agtcgtggtc tatttcaaaa cacctgaact gaatctgttt
    15901 aaagagtcgt ggcaggatct gccgcagaat aaactgacta tccgtctgca acctgatgaa
    15961 ggcgtggata tccaggtact gaataaagtt cctggccttg accacaaaca taacctgcaa
    16021 atcaccaagc tggatctgag ctattcagaa acctttaatc agacgcatct ggcggatgcc
    16081 tatgaacgtt tgctgctgga aaccatgcgt ggtattcagg cactgtttgt acgtcgcgac
    16141 gaagtggaag aagcctggaa atgggtagac tccattactg aggcgtgggc gatggacaat
    16201 gatgcgccga aaccgtatca ggccggaacc tggggacccg ttgcctcggt ggcgatgatt
    16261 acccgtgatg gtcgttcctg gaatgagttt gagtaatatc agcgcttatc ctttatggtt
    16321 attttaccgg taacatgatc ttgcacagat tgtagaacaa tttttacact ttcaggcctc
    16381 gtgcggattc acccacgagg ctttttttat tacactgact gaaacgtttt tgccctatga
    16441 gctccggtta caggcgtttc actcataaat cctctgaatg aaacgcgttg tgaatcatcc
    16501 tgctctgaca actcaatttc aggagccttt atgaatccac aattgttacg cgtaacaaat
    16561 cgaatcattg aacgttcgcg cgagactcgc tctgcttatc tcgcccggat agaacaagcg
    16621 aaaacgtcga ccgttcatcg ttcgcagttg gcatgcggta acctggcaca cggtttcgct
    16681 gcctgccagc cagaagacaa agcctctttg aaaagcatgt tgcgtaacaa tatcgccatc
    16741 atcacctcct ataacgacat gctctccgcg caccagcctt atgaacacta tccagaaatc
    16801 attcgtaaag ccctgcatga agcgaatgcg gttggtcagg ttgcgggcgg tgttccggcg
    16861 atgtgtgatg gtgtcaccca ggggcaggat ggaatggaat tgtcgctgct aagccgcgaa
    16921 gtgatagcga tgtctgcggc ggtggggctg tcccataaca tgtttgatgg tgctctgttc
    16981 ctcggtgtgt gcgacaagat tgtcccgggt ctgacgatgg cagccctgtc gtttggtcat
    17041 ttgcctgcgg tgtttgtgcc gtctggaccg atggcaagcg gtttgccaaa taaagaaaaa
    17101 gtgcgtattc gccagcttta tgccgaaggt aaagtggacc gcat
//
LOCUS       DAEEGA010000083        16387 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEEGA010000083 DAEEGA010000000
VERSION     DAEEGA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16387
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.083"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(85..2061)
                     /gene="speA"
                     /locus_tag="KLH46_003055"
     CDS             complement(85..2061)
                     /gene="speA"
                     /locus_tag="KLH46_003055"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBE0379656.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="KLH46_003056"
     CDS             complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="KLH46_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBE0379657.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2337..2552
                     /locus_tag="KLH46_003057"
     CDS             2337..2552
                     /locus_tag="KLH46_003057"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379658.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2856..4010
                     /gene="metK"
                     /locus_tag="KLH46_003058"
     CDS             2856..4010
                     /gene="metK"
                     /locus_tag="KLH46_003058"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBE0379659.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4447..5841
                     /gene="galP"
                     /locus_tag="KLH46_003059"
     CDS             4447..5841
                     /gene="galP"
                     /locus_tag="KLH46_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBE0379660.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5918..6415
                     /locus_tag="KLH46_003060"
     CDS             5918..6415
                     /locus_tag="KLH46_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBE0379661.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6510..7217
                     /gene="endA"
                     /locus_tag="KLH46_003061"
     CDS             6510..7217
                     /gene="endA"
                     /locus_tag="KLH46_003061"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBE0379662.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7297..8028
                     /gene="rsmE"
                     /locus_tag="KLH46_003062"
     CDS             7297..8028
                     /gene="rsmE"
                     /locus_tag="KLH46_003062"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBE0379663.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8041..8991
                     /gene="gshB"
                     /locus_tag="KLH46_003063"
     CDS             8041..8991
                     /gene="gshB"
                     /locus_tag="KLH46_003063"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBE0379664.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9100..9663
                     /locus_tag="KLH46_003064"
     CDS             9100..9663
                     /locus_tag="KLH46_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBE0379665.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIAGRWREAAKLIGVDILTMPGVAGHA"
     gene            9663..10079
                     /gene="ruvX"
                     /locus_tag="KLH46_003065"
     CDS             9663..10079
                     /gene="ruvX"
                     /locus_tag="KLH46_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBE0379666.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10255..11235)
                     /locus_tag="KLH46_003066"
     CDS             complement(10255..11235)
                     /locus_tag="KLH46_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBE0379667.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11253..11957
                     /gene="yggS"
                     /locus_tag="KLH46_003067"
     CDS             11253..11957
                     /gene="yggS"
                     /locus_tag="KLH46_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBE0379668.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11975..12541
                     /gene="yggT"
                     /locus_tag="KLH46_003068"
     CDS             11975..12541
                     /gene="yggT"
                     /locus_tag="KLH46_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBE0379669.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12538..12828
                     /locus_tag="KLH46_003069"
     CDS             12538..12828
                     /locus_tag="KLH46_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBE0379670.1"
                     /translation="MSAVTVNDDGLVLQLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12836..13429
                     /locus_tag="KLH46_003070"
     CDS             12836..13429
                     /locus_tag="KLH46_003070"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBE0379671.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13422..14558
                     /gene="hemW"
                     /locus_tag="KLH46_003071"
     CDS             13422..14558
                     /gene="hemW"
                     /locus_tag="KLH46_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBE0379672.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14872..15835
                     /locus_tag="KLH46_003072"
                     /pseudo
     CDS             14872..15835
                     /locus_tag="KLH46_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15880..16383
                     /locus_tag="KLH46_003073"
     CDS             15880..16383
                     /locus_tag="KLH46_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBE0379673.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4058 a         4220 c         4126 g         3983 t
ORIGIN      
        1 cagctagatt atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc
       61 gacccgccag cggaataatc ttaattattc atcttcaaga taagtataac cgtacaaacc
      121 tgcctcgaac tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac
      181 ttgatcgcgg aactgggtta acagcgtttt cggatcgagc tgtacatatt gcagcatgtc
      241 cgccacggta tcgccttcgt cagacagttc tacttctacg ctaccgtcag ggaacacgaa
      301 cacgtcaact gcttcggtat caccgaacag gttgtgcata ttgccaagga tctcctgata
      361 tgcaccgacc ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcattgg
      421 cattgtcgtg gcaataccat caccatcaat atagtggtcg atagcaccgt cagagtcaca
      481 ggtaatatcc agcagcacag cgcgacgttc cggcacttga tccagccctt ccagcggtag
      541 aaccgggaac aactggtcga tcccccatgc gtccggcatt gactggaaca gcgagaaatt
      601 gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg attatcggac gatgcgcacg
      661 gttttgcgga tccagttgct tctgcacttc atggcacatg ctcagataga gttgttctgc
      721 ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag
      781 atccatctgg ctgtcgtgta accattcacg caaagaacgg cgagttcccg gttcgtgcat
      841 ttcctgccag gtttcccaca tgctttgcag cgcacgcggc gcatcttcta cgggcgcggt
      901 cggcaccgta tattcgttac gttccacgcc gatgatattc gacaccagca cggtatggtg
      961 cgcagtcacc gcacggcccg attcggtgat taccgtcgga tgcggcagac cattttcttc
     1021 acacgcatcg ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac
     1081 ggagcagtca gactgcgaac gggtgccttc ataatccacg cccagaccgc cgccgacgtc
     1141 gaagcactga atattgacgc ccagcttgtg cagttccaca tagaaacgcg cggattcacg
     1201 aacgcctgtc gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag
     1261 ttgcaggctg tcgagacgcc cggcttcacg cagggtttca accagttgca gtacctgagt
     1321 cgcagccagg ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg
     1381 cgaagccaga cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc
     1441 cagcacaatg gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt
     1501 ctcgccaatt aatgccaagc ggatatattc gcggtcttta taaccgttgc agacgatgac
     1561 gctacgagtc atgccagcat gtgccagcac tgccatcaac tcggctttgg aaccggcttc
     1621 cagacccagc ggttcacccg aatgaatcag ggactcgatc acgcggcggt gctggttaac
     1681 tttgatcgga taaacaagga agtaatcgcc gttatagccg taggattccc tcgcacgttt
     1741 gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc
     1801 aggcagacgc tgaccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc
     1861 ttccgggacg tccgggtccg ggcacacgct gatgtggccc agctcgttaa cgtcatagta
     1921 gttattgccc caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct
     1981 cattgcaacc tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc
     2041 catagacatg tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta
     2101 acgactgtcg cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt
     2161 gtatgctgac gctccgactg cgcaaccggt ttctttttca taacattatt aagcacataa
     2221 ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg
     2281 cccttgttca gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg
     2341 gtataacatc ggcaggtatg caaagcagag atgcagagtg cggggaacga gtcttcacca
     2401 gaacggtgag acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc
     2461 cgcctgttaa gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa
     2521 cacgaaagaa acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa
     2581 tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca
     2641 gccacgtttt taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt
     2701 gacctaaaat agccatccag atgttaatcc atccataccg attaacactc agactgccag
     2761 tgtttttaac ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt
     2821 tgagctaacc aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg
     2881 agtccgtctc tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag
     2941 acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg
     3001 gcatggtttt agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca
     3061 cccgtaacac cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact
     3121 cctgtgcggt tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc
     3181 gtgccgatcc gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta
     3241 atgaaaccga cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc
     3301 aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg
     3361 tgactttcca gtatgacgac ggcaaaatcg ttggtattga tgctgtcgtg ctttccactc
     3421 agcactctga agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca
     3481 agccaattct gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg
     3541 gtcgtttcgt tatcggtggc ccaatgggtg actgcggtct gactggccgt aaaattatcg
     3601 ttgataccta cggcggcatg gcgcgtcacg gtggcggtgc attctctggt aaagatccat
     3661 caaaagtgga ccgttccgca gcctacgcag cacgttatgt cgcgaaaaac atcgttgctg
     3721 ctggcctggc cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga
     3781 cctccatcat ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc
     3841 tggtacgtga gttcttcgac ctgcgcccat acggactgat tcagatgctg gatctgctgc
     3901 acccgatcta caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg
     3961 aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg tctgaagtaa tctttcttca
     4021 cctgcgttca aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag
     4081 gcgttcacgc cgcatccggc aaaaaaactg cccgcacaat aacatcattc ttcctgatca
     4141 cgtttcacgg caaattacca tcacaactga aaccgattac accaaccaca ccagcaaaat
     4201 atttgtaata ttttcatatt attattcagc tttcacagtt gttacatttc ttttcagtaa
     4261 agtcttaatt gcagatgaca gcgtttaatc tatgatgata taactcaatt attttcatgc
     4321 ccttaaatca taactaagat aaatgttagt gtaagcgatt acactgatgt gatttgcttc
     4381 acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac tatattggag
     4441 ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac gtttttcgtc
     4501 tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt aattgctggc
     4561 gcactgccgt ttattgcaga tgaattccag ataacttcgc acacgcaaga atgggtcgta
     4621 agctccatga tgttcggtgc ggcagtcggt gcggtgggca tcggctggct ctcctttaaa
     4681 ctcgggcgca aaaagagcct gatgatcggc gcaatcctgt ttgttgccgg ttcactgttc
     4741 tctgcggctg cgccaaacgt tgaagtactg attctttcgc gcgttctgct ggggctggcg
     4801 gtgggtgtgg cctcttatac cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt
     4861 cgcggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct cggtgcttat
     4921 ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg tgtgattatc
     4981 atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc acgttggttt
     5041 gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg tgacaccagc
     5101 gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc
     5161 tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg cgtactgtta
     5221 caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc
     5281 gaactggcgg gttataccaa caccaccgag caaatgtggg ggacagtgat tgtcggcctg
     5341 accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg acgtaaacca
     5401 acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg tacaatgatg
     5461 catatcggta tccactctcc gtcggcgcag tatttcgcca tcgccatgct gctgatgttt
     5521 attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc cgaaattcag
     5581 ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg gattgccaac
     5641 atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc tgggtaacgc caacaccttc
     5701 tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct ggtaccggaa
     5761 accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg taaactgcgc
     5821 gaaatcggcg ctcacgatta atctcctcag gcttcctccc gtcgcggagg aagccgcctc
     5881 ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc gtctccctat
     5941 cgccatccaa caggccgtta tgcgccgcct gcgggaaaaa ctcgcccagg ccaacctgaa
     6001 gctagggcgt aactacccgg agccaaaact ctcttacacc cagcgcggaa cctccgccgg
     6061 aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt tggaaaacag
     6121 tgaagctttt attgaagaag tggtaccgca cgaactggca catttgctgg tatggaaaca
     6181 tttcggccgc gtagcgccac atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg
     6241 cgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca acaccttccc
     6301 ctaccgctgt aagtgccagg aacatcagct taccgtacgc cgccataatc gcgtagttcg
     6361 tggcgaggcc atctatcgct gtgttcactg cggtgaacag ctggttgcga aataaccacc
     6421 tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact ttcgctacgt
     6481 tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg
     6541 tactgagcgc agcattttcc ggcccgacgt tggccgaagg tatcaatagt ttttctcagg
     6601 cgaaagccgc ggcggtaaaa gtccacgctg atgcgcccgg tacgttttat tgcggatgta
     6661 aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc
     6721 gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca cgtcgttccc gcctggcaat
     6781 tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct aaagatccgg
     6841 tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggc gaggtgaatg
     6901 gcgatcgcgg caattttatg tacagccagt ggaatggcgg cgaaggccag tacggtcaat
     6961 gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg
     7021 ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc tctcgccagc
     7081 aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg gagtgcgagc
     7141 gcgatgaacg catcgcgaag gtgcagggca atcataaccc gtatgtgcaa cgcgcttgcc
     7201 aggcgcgaaa gagctaacct acactagcgg gattcttttt gttaacccct accccacgcg
     7261 tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg catttatcat
     7321 cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc caaccatatc
     7381 gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg tagcaaccag
     7441 gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa ggtgctggaa
     7501 ggccagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt gatgtcgcgt
     7561 ggtgaaaaaa tggaatttac tatccagaaa tcgatcgaac tcggtgtaag cctcattacg
     7621 ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa caagaagctt
     7681 cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg
     7741 gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga aggactgaaa
     7801 ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgcctttacc ggttgaacgc
     7861 gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc catgactgcc
     7921 cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga gacaactgcg
     7981 ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg gagaagaata
     8041 atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa gaaagattcc
     8101 agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta tatggagatg
     8161 ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag
     8221 cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc
     8281 gatgtgatcc tgatgcgtaa agacccgccg tttgataccg agtttatcta cgcgacctat
     8341 attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca gagcctgcgc
     8401 gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga aacgctggtt
     8461 acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat cattcttaag
     8521 ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc
     8581 ggcgtgattg ccgaaaccct gactgagcat ggcacttgct actgcatggc gcaaaattac
     8641 ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga gccggtaccg
     8701 tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc tgccggtggt
     8761 cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa tcgcccgtca gatcgggccg
     8821 acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga ccgtctgact
     8881 gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt tccggtgtcg
     8941 atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta acccacctta
     9001 gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg catactgggc
     9061 gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca gcatcacttt
     9121 cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt ctacatttgc
     9181 gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga aaatctcaaa
     9241 attgaaggga ttctggaaaa gctgaagatc acgccggagc cgcgtgatga atcaatccgt
     9301 ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt tattttgcat
     9361 actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt gatgaccact
     9421 tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt attggtggct
     9481 ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga taacgcgtgg
     9541 ctaacggccc cggcggatct gaatattctg ttcaaaacgc cgattgccgg ccgctggcgc
     9601 gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc aggacacgcc
     9661 tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc gtagcggtcg
     9721 gccaacgcat taccggcacc gctcgccctt tgcctgcaat taaagcgcag gacggtacgc
     9781 cggactggaa cctcatcgaa cgtttactga aagagtggca gcctgacgaa atcatcgtcg
     9841 gtttgccgct gaatatggac ggcaccgaac agccgttaac cgccagagcg cgcaaatttg
     9901 ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgag cgtcttagca
     9961 ctgtggaagc ccgttccggt ctttttgaac agggcggcta tcgggcgctc aacaaaggca
    10021 aagttgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag ggatattaag
    10081 gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg gcgtgaacgc
    10141 cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta gcgtcgcatc
    10201 agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc aacgtcacaa
    10261 acgcccttcc cccacccgct gctgataact ctgctgaaac gttaacatcc ccacctgctg
    10321 cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca aattccccac
    10381 cgcgggagtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt tatccacttc
    10441 cagcttttgt gacagcactg cccgtaaact acccgcaagt tgattacgta cgggatcttt
    10501 ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac cacgtgtatg
    10561 taaggttgcc agcaccagat gcccggtttc tgccgccgtc agcgccagac ggattgtctc
    10621 actgtcacgt agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa
    10681 ccccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca aacatcgctg
    10741 gctggcatag agatattcca caggatcttc cagcgtcaga atatgcgcat cggcatgttg
    10801 attaagatag ccaaccatcg ccgccagcgt ggtagatttg ccactccccg tagcccccgt
    10861 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gtgcaccaag
    10921 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
    10981 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacgg caaaatccag
    11041 ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc gcagtagctc
    11101 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattctg ccacgaatgc gccatcgtgc
    11161 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
    11221 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
    11281 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
    11341 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
    11401 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
    11461 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
    11521 gccgcctagt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
    11581 cccgcctcaa cgagcagcgc ccggcagaac ttccccctct taacgtactg attcaaatta
    11641 atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
    11701 cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg
    11761 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
    11821 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
    11881 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
    11941 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
    12001 aacggtcatt gagctgtata caatggtgct gttattacgc atctggatgc agtgggctca
    12061 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtagcgcagc caattatcgg
    12121 gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc tgctggttgc
    12181 ctatattctc agttttatca aagccattgt gctgtttgaa gtggtgacct tcctgccaat
    12241 catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga ttttctgggt
    12301 cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga ttgaatacgt
    12361 gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac cggcaatggg
    12421 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgccatca atatgggtgt
    12481 cgcagaagta ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg
    12541 agtgccgtaa cagttaatga tgacggtctg gttttacagc tctatattca gccgaaagcc
    12601 agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat taccgcgccg
    12661 ccggttgacg gccaggccaa cagtcatctg gtgaagtttc tcggtaagca attccgggtt
    12721 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
    12781 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
    12841 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
    12901 cgactttggt cttgatatcg tggcccaaac agacctcggc gttgattccg ctgaagaaac
    12961 cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
    13021 tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg gcgcgccggg
    13081 tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc tgcaaaaact
    13141 gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
    13201 ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca gctggccggg
    13261 cgtgattact cgcgaaccag cgggcactgg tggctttggt tatgatccaa tcttcttcgt
    13321 accttccgaa gggaaaaccg ccgccgaact gacccgcgaa gaaaagagcg ccatttccca
    13381 ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
    13441 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
    13501 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg ctttgcgatc
    13561 tggacaatga tgtggcttac gctcagggcc gtgaagtaaa gacaatcttt attggcggtg
    13621 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctagacggc gtgcgtgcgc
    13681 gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaacccaggc acggtagaag
    13741 cggatcgctt tgtcgattat cagcgtgctg gtgtaaaccg catctctatt ggtgtgcaga
    13801 gttttagcga agaaaagctg aaacgacttg ggcgtattca tggcccgcaa gaagcgaaac
    13861 gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat ttgatgcatg
    13921 gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc attgaactga
    13981 atccgccgca tctttcctgg tatcaactga ctatcgaacc taatacgctg tttggctcgc
    14041 gaccgccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
    14101 tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa cccggttatc
    14161 agtgccagca caatctcaac tactggcgct ttggtgacta catcggtatt ggctgcggcg
    14221 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccactaaa acgcgtcatc
    14281 ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa gccgcagata
    14341 agccgtttga gttctttatg aatcgcttcc gtctgctgga ggccgcgccg cgcgtggagt
    14401 ttattgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac gaggcgattg
    14461 cccagggtta tctcaccgaa tgtgcggatt actggcagat aacggaacat ggtaaattat
    14521 tcttaaactc acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc
    14581 attaatcact tcttttatcc ccccgttaat taacctcggc agaaatatat ttcttactga
    14641 ataaatatcg acgacattta ttatatatat ttagtgaata tttactaatc attcattgat
    14701 tataaatatg tacaacctgg cgcacaaatt taatatttaa tttctgactt tatttcaagt
    14761 tgtgataaac atagacaagt tgtcagacag gttaaccaat ttgtcgcaca aatcactgat
    14821 aagcacaatt agatatcaac caatttccat ccttcaacag ggagtttttt tatgaaaatg
    14881 aggtcattta cccgctcatt agtctgcgca tccttgctgg ctctggttag cactggcgta
    14941 aacgccgcag aaaaagtggc gttaaagtta gcacacaacc tggaaagaag tcatgttgta
    15001 catcagagtt ttgaagagct ggcgaaagag gtaaaacagc tgtcaaaagg taatatggtg
    15061 atccgtattt atcccagcag ccagatgggt aatgcgcgag aaactatgga attgttgcaa
    15121 aatggcgcac tggatatgac caaaggttcc gccagtgatc tggagtcttt tgataatatt
    15181 tatgctatct ataatttgcc gttcttatta agttgttttt ggtgaagttg gtaaagaaat
    15241 tatggactca acaaaagaca aaggcttttt tgctttatca gcttatgtag cgggtactcg
    15301 cagtttctat gcgaaaaaac cgattacaaa gccagaagat cttaaaggac tgaaaattcg
    15361 tgttcagcca agtccgacca ccatcaaaat gattgaactg atgggtggtt cacccactcc
    15421 aatttcattt ggcgaagttt atactgcgat gcagcaaggt gtagtagatg gcgcagaaaa
    15481 taacgtgcct tcctggatgc agactcgaca tattgaaatt gccaaagttt tttctgaaga
    15541 tgaacacgcc tcaattcctg actttttggt tatctccacc aaaacatgga ataaattaac
    15601 cccagaacag caacaaatcc tcgaaacagc ggcgaagaaa tccgaagctt atcaacaaaa
    15661 attgtgggaa aaaattgacg ctgacacacg tgctcaggcg aaagctatgg ggggtgaaat
    15721 tgtaaaagtg gataaagcgc cattccgtaa agccgtgcaa cctctgtttg acgacttcaa
    15781 aaaagatcca aaacaagctg ctttgttaga gaaattcgac aacgctgctc aataatattc
    15841 cgggccagcc tctggccctg attttccagg atgcttacaa tgatatttaa ccgcctgaaa
    15901 cttgcggtag accgcgtcat tgccgcgttt tctgtcgccg taatgcttgc tctggttgta
    15961 tgcgtagtct ggcaagtatt tagccgctac gtacttaacc agccaagtac attaaccgat
    16021 gagctggcac gctttttgat gatctgggta ggattattag gtgctgcata taccgtcggc
    16081 gcgcagcgac atctctctat tgatttattt gctctcgccc tgaataagcg caaacaatta
    16141 cttcttagta tagtcattaa cgttctcatt ttaggttttg ccggttcggt cattgtgact
    16201 ggtggcctga aattaattga taaaacactg gcaacatcac aggtatctgc cgctatgcaa
    16261 ataccgatgg gatacgtata tatcattctg ccattaagtg gagtggtaat gatgttttat
    16321 gccttgtgtt ttattaatca aagcattcaa caactgaaac aacctgcgca ggaggccagt
    16381 taatgga
//
LOCUS       DAEEGA010000084        16098 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEEGA010000084 DAEEGA010000000
VERSION     DAEEGA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16098
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.084"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..588
                     /gene="tuf"
                     /locus_tag="KLH46_003074"
     CDS             <1..588
                     /gene="tuf"
                     /locus_tag="KLH46_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBE0379674.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            771..965
                     /gene="bfd"
                     /locus_tag="KLH46_003075"
     CDS             771..965
                     /gene="bfd"
                     /locus_tag="KLH46_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBE0379675.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1038..1514
                     /gene="bfr"
                     /locus_tag="KLH46_003076"
     CDS             1038..1514
                     /gene="bfr"
                     /locus_tag="KLH46_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBE0379676.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1511..1978)
                     /locus_tag="KLH46_003077"
     CDS             complement(1511..1978)
                     /locus_tag="KLH46_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBE0379677.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            2359..2670
                     /gene="rpsJ"
                     /locus_tag="KLH46_003078"
     CDS             2359..2670
                     /gene="rpsJ"
                     /locus_tag="KLH46_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBE0379678.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2703..3332
                     /gene="rplC"
                     /locus_tag="KLH46_003079"
     CDS             2703..3332
                     /gene="rplC"
                     /locus_tag="KLH46_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBE0379679.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3343..3948
                     /gene="rplD"
                     /locus_tag="KLH46_003080"
     CDS             3343..3948
                     /gene="rplD"
                     /locus_tag="KLH46_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBE0379680.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3945..4247
                     /gene="rplW"
                     /locus_tag="KLH46_003081"
     CDS             3945..4247
                     /gene="rplW"
                     /locus_tag="KLH46_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBE0379681.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4265..5086
                     /gene="rplB"
                     /locus_tag="KLH46_003082"
     CDS             4265..5086
                     /gene="rplB"
                     /locus_tag="KLH46_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBE0379682.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5103..5381
                     /gene="rpsS"
                     /locus_tag="KLH46_003083"
     CDS             5103..5381
                     /gene="rpsS"
                     /locus_tag="KLH46_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBE0379683.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5396..5728
                     /gene="rplV"
                     /locus_tag="KLH46_003084"
     CDS             5396..5728
                     /gene="rplV"
                     /locus_tag="KLH46_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBE0379684.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5746..6447
                     /gene="rpsC"
                     /locus_tag="KLH46_003085"
     CDS             5746..6447
                     /gene="rpsC"
                     /locus_tag="KLH46_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBE0379685.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6460..6870
                     /gene="rplP"
                     /locus_tag="KLH46_003086"
     CDS             6460..6870
                     /gene="rplP"
                     /locus_tag="KLH46_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBE0379686.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6870..7061
                     /gene="rpmC"
                     /locus_tag="KLH46_003087"
     CDS             6870..7061
                     /gene="rpmC"
                     /locus_tag="KLH46_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBE0379687.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7061..7315
                     /gene="rpsQ"
                     /locus_tag="KLH46_003088"
     CDS             7061..7315
                     /gene="rpsQ"
                     /locus_tag="KLH46_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBE0379688.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7480..7851
                     /gene="rplN"
                     /locus_tag="KLH46_003089"
     CDS             7480..7851
                     /gene="rplN"
                     /locus_tag="KLH46_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBE0379689.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7862..8176
                     /gene="rplX"
                     /locus_tag="KLH46_003090"
     CDS             7862..8176
                     /gene="rplX"
                     /locus_tag="KLH46_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBE0379690.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8191..8730
                     /gene="rplE"
                     /locus_tag="KLH46_003091"
     CDS             8191..8730
                     /gene="rplE"
                     /locus_tag="KLH46_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBE0379691.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8745..9050
                     /gene="rpsN"
                     /locus_tag="KLH46_003092"
     CDS             8745..9050
                     /gene="rpsN"
                     /locus_tag="KLH46_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBE0379692.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9084..9476
                     /gene="rpsH"
                     /locus_tag="KLH46_003093"
     CDS             9084..9476
                     /gene="rpsH"
                     /locus_tag="KLH46_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBE0379693.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9489..10022
                     /gene="rplF"
                     /locus_tag="KLH46_003094"
     CDS             9489..10022
                     /gene="rplF"
                     /locus_tag="KLH46_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBE0379694.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10032..10385
                     /gene="rplR"
                     /locus_tag="KLH46_003095"
     CDS             10032..10385
                     /gene="rplR"
                     /locus_tag="KLH46_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBE0379695.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10400..10903
                     /gene="rpsE"
                     /locus_tag="KLH46_003096"
     CDS             10400..10903
                     /gene="rpsE"
                     /locus_tag="KLH46_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBE0379696.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10907..11086
                     /gene="rpmD"
                     /locus_tag="KLH46_003097"
     CDS             10907..11086
                     /gene="rpmD"
                     /locus_tag="KLH46_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBE0379697.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11090..11524
                     /gene="rplO"
                     /locus_tag="KLH46_003098"
     CDS             11090..11524
                     /gene="rplO"
                     /locus_tag="KLH46_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBE0379698.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11532..12863
                     /gene="secY"
                     /locus_tag="KLH46_003099"
     CDS             11532..12863
                     /gene="secY"
                     /locus_tag="KLH46_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBE0379699.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12895..13011
                     /gene="rpmJ"
                     /locus_tag="KLH46_003100"
     CDS             12895..13011
                     /gene="rpmJ"
                     /locus_tag="KLH46_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBE0379700.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13158..13514
                     /gene="rpsM"
                     /locus_tag="KLH46_003101"
     CDS             13158..13514
                     /gene="rpsM"
                     /locus_tag="KLH46_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBE0379701.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13531..13920
                     /gene="rpsK"
                     /locus_tag="KLH46_003102"
     CDS             13531..13920
                     /gene="rpsK"
                     /locus_tag="KLH46_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBE0379702.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13954..14574
                     /gene="rpsD"
                     /locus_tag="KLH46_003103"
     CDS             13954..14574
                     /gene="rpsD"
                     /locus_tag="KLH46_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBE0379703.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14600..15589
                     /gene="rpoA"
                     /locus_tag="KLH46_003104"
     CDS             14600..15589
                     /gene="rpoA"
                     /locus_tag="KLH46_003104"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBE0379704.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15630..16013
                     /gene="rplQ"
                     /locus_tag="KLH46_003105"
     CDS             15630..16013
                     /gene="rplQ"
                     /locus_tag="KLH46_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBE0379705.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4143 a         3786 c         4291 g         3878 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aaaccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt acacgttaat
      601 tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat ttgagctcga
      661 aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt actattgtaa
      721 tcataaccat tctcatttac actttgtgca gaatttgaac gggagcaatc atgtacgttt
      781 gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag tttcatccgc
      841 aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt aagtgcattc
      901 gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt aaagagattg
      961 cctgaagccc acgcttattt tttgacatcc tgctagcccc atctacgctc taatagtgga
     1021 agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc aataaactat
     1081 tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg tttaaaaact
     1141 ggggcctgat gcgtctcaat gacgtcgaat accatgaatc catcgatgag atgaaacacg
     1201 ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta caggatttag
     1261 gcaaactggg gattggtgaa gatgtcgagg agatgctgca atctgacctg cgattagaac
     1321 tggaaggcgc gaaggatctg cgtgaggcta tcgcatatgc cgacagcgtt catgactatg
     1381 ttagccgcga tatgatgatc gagatccttg ctgatgaaga aggtcatatc gactggctcg
     1441 aaactgaact ggatctcatc ggtaaaatcg gcctacaaaa ttacctgcaa tcacaaatta
     1501 aagttaaaga ttaacggcct atcagcaaac tatcccagcc tataacgaaa cccgcagccg
     1561 ccagaaatgg cccaaaaggc agcgggtttt ttaatgattc ttttcctctc atgagcagac
     1621 caataactac ggctccgcaa gcgaatgagg cagcaaggaa aaccaatcgt ggtaagaagg
     1681 cccatgaatg ccaggcgcca agggcggcga gaaacttcac atcaccatag cctaatcctt
     1741 ctttatgtcg cagtatgcga tacccccagt aaatgacagc gaatgtgccg taaccaacaa
     1801 ttgcccccca taacgcatcg gctaaaccat caggatgaca aacttgatag aacaaaagac
     1861 ctgaccagag taacgggcac gtaaatctgt caggtaacaa gccatgcttt gcatcccaga
     1921 aaaaaagtag ggctgataaa caagcgtaca aaattagaaa gggaagggta gtgtccataa
     1981 aacatcctct gtaattggag tatcaccatc gaataactcc ggatgctcag caacagagag
     2041 agagcgctga tttgttaacc tgattccggt gaaaaacgct atcttcacaa ccttacaaat
     2101 ttcgtggaat gcagctcgca atctgcgcgt tgcgattaat ttctacccga tgcttgcgtc
     2161 aggctcagat ttgcgtataa tgcgcgggct tgtcgtagtt gacagcaggt tcaatctgaa
     2221 cctcagttag ccgaatttgg ctacctaaca atgctcccaa tcggggagct acgtaagaac
     2281 ggttacactc tcccatcaat cgtaatgggt ctgaggagta atcattttcg tttataaaat
     2341 aattggagct ctggtctcat gcagaaccaa agaatccgta tccgcctgaa agcgtttgat
     2401 catcgtctga tcgatcaagc aaccgcggaa atcgtcgaga ctgccaagcg cactggtgcg
     2461 caggtccgtg gtccgatccc gctgccgaca cgcaaagagc gcttcactgt tctgatctcc
     2521 ccgcacgtca acaaagacgc gcgcgatcag tacgaaatcc gtactcactt gcgtctggtt
     2581 gacatcgttg agccaaccga gaaaaccgtt gatgctctga tgcgtctgga tctggctgcc
     2641 ggtgtagacg tgcagatcag cctgggttaa tcaggtcatt gagcgattga gaggttgaaa
     2701 caatgattgg tttagtcggt aaaaaagtgg gtatgacccg tatcttcaca gaagacggcg
     2761 tttctatccc agtaaccgta atcgaagttg aagcaaaccg cgttactcag gttaaagacc
     2821 tggctaacga tggctaccgt gctattcagg tgaccaccgg tgctaaaaaa gctaaccgtg
     2881 tgaccaagcc tgaagctggc cacttcgcta aagctggcgt agaagctggc cgtggtctgt
     2941 gggaattccg cctggctgaa ggcgaagagt tcactgtagg tcagagcatt agcgttgaac
     3001 tgtttgctga cgttaaaaaa gttgacgtaa ctggcacctc taaaggtaaa ggtttcgcag
     3061 gtaccgttaa gcgctggaac ttccgtaccc aggacgctac tcacggtaac tccttgtctc
     3121 accgcgttcc gggttctatc ggtcagaacc agactccggg caaagtgttc aaaggcaaga
     3181 aaatggcagg tcagatgggt aacgaacgtg taaccgttca gagccttgac gtagtacgcg
     3241 ttgacgctga gcgcaacctg ctgctggtta aaggtgctgt cccgggtgca accggtagcg
     3301 acctgatcgt taaaccagct gtgaaggcgt aaggagatag caatggaatt agtattgaaa
     3361 gacgcgcaga gcgcgctgac tgtttccgaa actaccttcg gtcgtgattt caacgaagcg
     3421 ctggttcacc aggttgttgt tgcttatgca gctggtgctc gtcagggtac tcgtgctcag
     3481 aagactcgtg ctgaagtaac tggttccggt aaaaaaccgt ggcgccagaa aggcaccggc
     3541 cgtgcgcgtt ctggttctat caagagcccg atctggcgtt ctggtggcgt gacctttgct
     3601 gctcgtccgc aggaccacag tcaaaaagtt aacaagaaga tgtaccgcgg cgcgctgaaa
     3661 agcatcctgt ccgaactggt acgtcaggat cgtctgatcg ttgtcgagaa gttctctgta
     3721 gaagcgccga aaactaagct gctggcacag aaactgaaag acatggctct ggaagatgtg
     3781 ctgatcatca ccggtgagct ggacgaaaac ctgttcctgg ctgcgcgcaa cctgcagaag
     3841 gttgacgtac gcgatgcaac tggtatcgac ccggttagcc tgatcgcctt cgacaaagtc
     3901 gtaatgactg ctgatgctgt taagcaagtt gaggagatgc tggcatgatt cgtgaagaac
     3961 gtctgctgaa ggtgctgcgt gcaccgcacg tttctgaaaa agcgtctact gcgatggaaa
     4021 aatccaacac catcgtactc aaagttgcga aagacgcgac caaagcagaa atcaaagctg
     4081 ctgtgcagaa actgtttgaa gtcgaagtcg aagtcgttaa caccctggta gttaaaggga
     4141 aagttaaacg tcacggacag cgtatcggtc gtcgtagcga ctggaaaaaa gcttacgtca
     4201 ccctgaaaga aggccagaat ctggacttcg ttggcggcgc tgagtaagtc ggaggagtaa
     4261 tacaatggca gttgttaaat gtaaaccgac atctccgggt cgtcgccacg tagttaaagt
     4321 ggttaaccct gagctgcaca agggcaaacc ttttgctccg ttgctggaaa aaaacagcaa
     4381 atccggtggt cgtaacaaca atggccgtat caccactcgt catatcggtg gtggccacaa
     4441 gcaggcttac cgtattgttg acttcaaacg caacaaagac ggtatcccgg cagttgttga
     4501 acgtcttgag tacgatccga accgttccgc gaacatcgcg ctggttctgt acaaagacgg
     4561 tgaacgccgt tacatcctgg cccctaaagg cctgaaagct ggcgaccaga ttcagtctgg
     4621 cgttgatgct gcaatcaaac caggtaacac cctgccgatg cgcaacatcc cggttggttc
     4681 tactgttcat aacgtagaaa tgaaaccagg taaaggcggt cagctggcac gttccgctgg
     4741 tacttacgtt cagatcgttg ctcgtgatgg tgcttatgtc accctgcgtc tgcgttctgg
     4801 tgaaatgcgt aaagtagaag cagactgccg tgcaactctg ggcgaagttg gcaatgctga
     4861 gcatatgctg cgcgttctgg gtaaagcagg tgctgcacgc tggcgtggtg ttcgtccgac
     4921 cgttcgcggt accgcgatga acccggtaga ccacccacat ggtggtggtg aaggtcgtaa
     4981 ctttggtaag cacccggtaa ctccgtgggg cgttcagacc aaaggtaaga agacccgcag
     5041 caacaagcgt actgataaat tcatcgtacg tcgccgtagc aaataatttt agaggataag
     5101 ccatgccacg ttctctcaag aaaggtcctt ttattgacct gcacttgctg aagaaggtag
     5161 agaaagcggt ggaaagcgga gacaagaagc ccctgcgcac ttggtcccgt cgttcaacga
     5221 tctttcctaa catgatcggt ttgaccatcg ctgtccataa tggtcgtcag cacgttccgg
     5281 tatttgtaac cgacgaaatg gtcggtcaca aactgggtga attcgcaccg actcgtactt
     5341 atcgcggcca cgctgctgat aaaaaagcga agaagaaata aggtaggagg aagagatgga
     5401 aactattgct aaacatcgcc atgctcgttc ttctgctcag aaggttcgcc ttgttgctga
     5461 cctgattcgc ggtaagaaag tgtcgcaggc tctggatatt ttgacctaca ccaacaagaa
     5521 agcggctgta caggtcaaga aagttctgga atctgccatt gctaacgctg aacacaacga
     5581 tggcgctgac attgacgatc tgaaagttac gaaaattttc gtagacgaag gcccgagcat
     5641 gaagcgcatt atgccgcgtg caaaaggtcg tgcagatcgc atcctgaagc gcaccagcca
     5701 catcactgtg gttgtgtccg atcgctgaga ctctggagac tagcaatggg tcagaaagta
     5761 catcctaatg gtattcgctt gggtattgta aaaccatgga actctacttg gtttgcgaac
     5821 accaaagaat tcgctgacaa cctggacagc gattttaaag tacgtcagta cctgactaag
     5881 gaactggcta aagcgtccgt atctcgtatc gttatcgagc gtccggctaa gagcatccgt
     5941 gtaaccattc acactgctcg cccgggtatc gttatcggta aaaaaggtga agacgtagaa
     6001 aaactgcgta aggtcgtagc ggacatcgct ggcgttcctg cacagatcaa catcgccgaa
     6061 gttcgtaagc ctgaactgga cgcaaaactg gttgctgaca gcatcacttc tcagctggaa
     6121 cgtcgcgtta tgttccgtcg tgctatgaag cgtgctgtac agaacgcaat gcgtctgggc
     6181 gctaaaggta ttaaagttga agttagcggc cgtctgggcg gcgcggaaat cgcacgtacc
     6241 gaatggtacc gcgaaggtcg cgtaccgctg cacactctgc gtgctgacat cgactacaac
     6301 acctctgaag cgcacaccac ttacggtgta atcggcgtta aagtgtggat cttcaaaggc
     6361 gagatcctgg gtggtatggc tgctgttgaa caaccggaaa aaccggctgc tcagcctaaa
     6421 aagcagcagc gtaaaggccg taaataagga gcgtcgctga tgttacaacc aaagcgtaca
     6481 aaattccgta aaatgcacaa aggccgtaac cgcggtctgg cgcagggtac ggatgttagc
     6541 ttcggcagct tcggtctgaa agctgttggc cgtggtcgtc tgactgcccg tcagatcgaa
     6601 gcagcacgtc gtgctatgac ccgtgcagtt aagcgtcaag gtaagatctg gatccgtgtg
     6661 ttcccggaca aaccgatcac tgaaaagccg ctggcagtgc gtatgggtaa aggtaaaggt
     6721 aacgtggagt attgggttgc cttgattcag ccgggtaaag tcctgtatga aatggacggt
     6781 gttccggaag agctggcccg tgaagcattc aagctggcag cagcgaaact gccgattaaa
     6841 accacctttg taactaagac ggtgatgtaa tgaaagcaaa agagctgcgt gagaagagca
     6901 ttgaagagct gaacaccgag ctgctgaacc tgctgcgtga gcagttcaac ctgcgtatgc
     6961 aggctgcaag tggccagctg caacagtctc acctgttgaa gcaagtgcgt cgcgatgtcg
     7021 cacgcgttaa gactttactg aacgagaagg cgggtgcgta atgaccgata aaatccgtac
     7081 tctgcaaggt cgcgttgtta gcgacaaaat ggagaaatcc attgttgttg ctatcgaacg
     7141 ttttgtgaaa cacccgatct acggtaaatt catcaagcgt acgaccaaac tgcacgtaca
     7201 tgacgagaac aacgaatgcg gtatcggtga cgtggttgaa atccgcgaat gccgtccgct
     7261 gtccaagact aaatcctgga cgctggttcg cgttgtagag aaagcggttc tgtaatacag
     7321 tacactctct cgatacgaat aaacggctca gaaatgagcc gtttattttt tctacccata
     7381 tccttgaagc ggtgttataa tgccgcgccc tcgatatggg gatttttaac gacctgattt
     7441 tcgggtctca gtagtagttg acattagcgg agcactaaaa tgatccaaga acagactatg
     7501 ctgaacgtcg ccgacaactc cggtgcacgt cgcgtaatgt gtatcaaggt tctgggtggc
     7561 tcgcaccgtc gctacgcagg cgtaggcgac atcatcaaga tcaccatcaa agaagcaatt
     7621 ccgcgtggta aggtcaaaaa aggtgatgtg ctgaaggcgg tagtggtgcg caccaagaag
     7681 ggtgttcgtc gcccggacgg ttctgtcatt cgcttcgatg gtaatgcttg tgttcttctg
     7741 aacaacaaca gcgagcagcc tatcggtacg cgtatttttg ggccggtaac tcgtgagctt
     7801 cgtagtgaga agttcatgaa aattatctct ctggcaccag aagtactcta aggagcgaat
     7861 catggcagcg aaaatccgtc gtgatgacga agttatcgtg ttaaccggta aagataaagg
     7921 taaacgcggt aaagttaaga atgtcctgtc ttccggcaag gtcattgttg aaggtatcaa
     7981 cctggttaag aaacatcaga agccggttcc ggccctgaac caaccgggtg gcatcgttga
     8041 aaaagaagcc gctattcagg tttccaacgt agcaatcttc aatgcggcaa ccggcaaggc
     8101 tgaccgtgta ggctttagat tcgaagacgg caaaaaagtc cgtttcttca agtctaacag
     8161 cgaaactatc aagtaatttg gagtagtacg atggcgaaac tgcatgatta ctacaaagac
     8221 gaagtagtta aaaaactcat gactgagttt aactacaatt ctgtcatgca agtccctcgg
     8281 gtcgagaaga tcaccctgaa catgggtgtt ggtgaagcga tcgctgacaa aaaactgctg
     8341 gataacgcag cagcagacct ggcagcaatc tccggtcaaa aaccgctgat caccaaagca
     8401 cgcaaatctg ttgcaggctt caaaatccgt cagggctatc cgatcggctg taaagtaact
     8461 ctgcgtggcg aacgcatgtg ggagttcttt gagcgcctga tcactattgc tgtacctcgt
     8521 atccgtgact tccgtggcct gtccgctaag tctttcgacg gtcgtggtaa ctacagcatg
     8581 ggtgtccgtg agcagatcat cttcccagaa atcgactacg ataaagtcga ccgcgttcgt
     8641 ggtttggata ttaccattac cactactgcg aaatctgacg aagaaggccg cgctctgctg
     8701 gctgcctttg acttcccgtt ccgcaagtaa ggtagggtta ctaaatggct aagcaatcaa
     8761 tgaaagcacg cgaagtaaaa cgcgtagctt tagctgataa atacttcgcg aaacgcgctg
     8821 aactgaaagc gatcatctct gatgtgaacg cttccgacga agatcgttgg aacgctgttc
     8881 tcaagctgca gactctgccg cgtgattcca gcccgtctcg tcagcgtaac cgctgccgtc
     8941 aaacaggtcg tccgcatggt ttcctgcgga agttcgggtt gagccgtatt aaggtccgtg
     9001 aagccgctat gcgcggtgaa atcccgggtc tgaaaaaggc tagctggtaa ttgtcaccaa
     9061 ttgaatcacg ggagtaaaga cagatgagca tgcaagatcc gatcgcggat atgctgaccc
     9121 gtatccgtaa cggtcaggcc gcgaacaaag ctgcggtcac catgccttcc tccaagctga
     9181 aagtggcaat cgccaacgtg ctgaaggaag aaggttttat tgaagatttt aaagttgaag
     9241 gcgacaccaa gcctgaactg gaacttactc tgaagtattt ccagggcaaa gctgttgtag
     9301 aaagcattca gcgtgtcagc cgcccaggtc tgcgcatcta taaacgtaaa gatgagctgc
     9361 cgaaagttat ggcgggtctg ggtatcgcag ttgtttctac ctctaaaggt gttatgactg
     9421 atcgtgcagc gcgccaggct ggtcttggtg gcgaaattat ctgctacgta gcctaatcgg
     9481 aggaaaaaat gtctcgtgtt gctaaagcac cggtcgttgt tcctgccggc gttgacgtaa
     9541 aaatcaacgg tcaggttatt acgatcaaag gtaaaaacgg cgagctgact cgtactctca
     9601 acgatgctgt tgaagttaaa catgcagata ataccctgac cttcggtccg cgtgatggtt
     9661 acgcagacgg ttgggcacag gctggtaccg cgcgtgccct gctgaactca atggttatcg
     9721 gtgttaccga aggcttcact aagaagctgc agctggttgg tgtaggttac cgtgcagcgg
     9781 ttaaaggcaa tgtgattaac ctgtctctgg gtttctctca tcctgttgac catcagctgc
     9841 ctgcgggtat cactgctgaa tgtccgactc agactgaaat cgtgctgaaa ggcgctgata
     9901 agcaggtgat cggccaggtt gcagcggatc tgcgcgccta ccgtcgtcct gagccttaca
     9961 aaggcaaggg tgttcgttac gccgacgaag tcgtgcgtac caaagaggct aagaagaagt
    10021 aaggtaacac tatggataag aaatctgctc gtatccgtcg tgcgacccgc gcacgccgca
    10081 agctccagga gctgggcgca actcgcctgg tggtacatcg taccccgcgt cacatttacg
    10141 cacaggtaat tgcaccgaac ggttctgaag ttctggtagc tgcttctact gtagaaaaag
    10201 ctatcgctga acaactgaag tacaccggta acaaagacgc ggctgcagct gtgggtaaag
    10261 ctgtcgctga acgcgctctg gaaaaaggca tcaaagatgt atcctttgac cgttccgggt
    10321 tccaatatca tggtcgtgtc caggcactgg cagatgctgc ccgtgaagct ggccttcagt
    10381 tctaaggtag aggtgtaaga tggctcacat cgaaaaacaa gctggcgaac tgcaggaaaa
    10441 gctgatcgcg gtaaaccgcg tatctaaaac cgttaaaggt ggtcgtattt tctccttcac
    10501 agctctgact gtagttggcg atggtaacgg tcgcgttggt tttggttacg gtaaagcgcg
    10561 tgaagttcca gcagcgatcc agaaagcgat ggaaaaagcc cgtcgcaata tgattaacgt
    10621 cgcgctgaat aacggcactc tacaacaccc tgttaaaggt gttcacacgg gttctcgcgt
    10681 attcatgcag ccggcttccg aaggtaccgg tatcatcgcc ggtggtgcaa tgcgcgccgt
    10741 tctggaagtc gctggggttc ataacgttct ggctaaagca tatggttcca ccaacccgat
    10801 caacgtggtt cgtgcaacta ttgatggcct ggaaaatatg aattctccag aaatggtcgc
    10861 tgccaagcgt ggtaaatccg ttgaagaaat tctggggaaa taaaccatgg caaagactat
    10921 taaaattact caaacccgca gtgcaatcgg tcgtctgccg aaacacaagg caacgctgct
    10981 tggcttgggt ctgcgtcgta ttggtcacac cgtagagcgc gaggatactc ctgctattcg
    11041 cggtatgatc aacgcggttt ccttcatggt taaagttgag gagtaagaga tgcgtttaaa
    11101 tactctgtct ccggccgaag gctccaaaaa ggcgggtaaa cgcctgggtc gtggtatcgg
    11161 ttctggcctc ggtaaaaccg gtggtcgtgg tcacaaaggt cagaagtctc gttctggcgg
    11221 tggcgtacgt cgcggtttcg agggtggtca gatgcctctg taccgtcgtc tgccgaaatt
    11281 cggcttcact tctcgtaaag cagcgattac agccgaagtt cgtctatctg acctggctaa
    11341 agtagaaggc ggtgttgtag acctgaacac gctgaaagcg gctaacatta tcggtatcca
    11401 gatcgagttc gcgaaagtga tcctggctgg cgaagtaacg actccggtaa ctgttcgtgg
    11461 cctgcgtgtt actaaaggcg ctcgtgctgc tatcgaagct gctggcggta aaatcgagga
    11521 ataagtagca gatggctaaa caaccgggat tagattttca aagtgccaaa ggtggcttag
    11581 gcgagctgaa acgcagactg ctgtttgtta tcggtgcgct gattgtgttc cgtattggct
    11641 cttttattcc gatccctggt attgatgccg ctgtacttgc caaactgctt gagcaacagc
    11701 gaggcaccat cattgagatg tttaacatgt tctctggtgg tgctctcagc cgtgcttcta
    11761 tctttgctct ggggatcatg ccgtatattt cggcgtcgat cattatccag ctgctgacgg
    11821 tggttcaccc aacgttggca gaaattaaga aagaagggga gtctggtcgt cgtaagatca
    11881 gccagtacac ccgttacggt actctggtgc tggcaatatt ccagtcgatc ggtattgcta
    11941 ccggtctgcc gaatatgcct ggtatgcaag gcctggtgat taacccgggc tttgcattct
    12001 acttcaccgc tgttgtaagt ctggtcacag gaacgatgtt cctgatgtgg ttgggcgaac
    12061 agatcactga acgaggtatc ggcaacggta tttcaatcat tatcttcgcc ggtattgtcg
    12121 cgggactccc gccagccatt gcccatacta tcgagcaagc gcgtcaaggc gacctgcact
    12181 tcctcgtgtt gctgttggtt gcagtattag tatttgcagt gacgttcttt gttgtgtttg
    12241 ttgagcgtgg tcaacgccgc attgtggtaa actacgcgaa acgtcagcaa ggtcgtcgtg
    12301 tctatgctgc acagagcaca catttaccgc tgaaagtgaa tatggcgggg gtaatcccgg
    12361 caatcttcgc ttccagtatt attctgttcc cggcgaccat cgcgtcatgg ttcgggggcg
    12421 gtactggttg gaactggctg acaacaattt cgctgtattt gcagcctggg caaccgcttt
    12481 atgtgttact ctatgcgtct gcaatcatct tcttctgttt cttctacacg gcgttggttt
    12541 tcaacccgcg tgaaacagca gataacctga agaagtccgg tgcatttgta ccaggaattc
    12601 gtccgggaga gcaaacggcg aagtatatcg ataaagtaat gacccgcctg accttggttg
    12661 gtgcgctgta tattaccttt atctgcctga tcccggagtt catgcgtgat gcaatgaaag
    12721 taccgttcta cttcggtggg acctcactgc ttatcgttgt tgtcgtgatt atggacttta
    12781 tggctcaagt gcaaactctg atgatgtcca gtcagtatga gtctgcattg aagaaggcga
    12841 acctgaaagg ctacggccga taattggtcg cccgagaagt tacggagagt aaaaatgaaa
    12901 gttcgtgctt ccgtcaagaa attatgccgt aactgcaaaa tcgttaagcg tgatggtgtc
    12961 atccgtgtga tttgcagtgc cgagccgaag cataaacagc gccaaggctg attttttcgc
    13021 atatttttct tgcaaagttg ggttgagctg gctagattag ccagccaatc ttttgtatgt
    13081 ctgtacgttt ccatttgagt atcctgaaaa cgggcttttc agcatggaac gtacatatta
    13141 aatagtagga gtgcatagtg gcccgtatag caggcattaa cattcctgat cataagcatg
    13201 ccgtaatcgc attaacttcg atttatggcg tcggcaagac ccgttctaaa gccatcctgg
    13261 ctgcagcggg tatcgctgaa gatgttaaga tcagtgagct gtctgaagga caaatcgaca
    13321 cgctgcgtga cgaagttgcc aaatttgtcg ttgaaggtga tctgcgccgt gaaatcagca
    13381 tgagcatcaa gcgcctgatg gatcttggtt gctatcgcgg tttgcgtcat cgtcgtggtc
    13441 tcccggttcg cggtcagcgt accaagacca acgcacgtac ccgtaagggt ccgcgcaaac
    13501 cgatcaagaa ataatcgggg tgattgaata atggcaaagg caccaattcg tgcacgtaaa
    13561 cgtgtaagaa aacaagtctc tgacggcgtg gctcatatcc atgcttcttt caacaacacc
    13621 atcgtgacta tcactgatcg tcagggtaac gcgttgggtt gggcaacagc cggtggttcc
    13681 ggtttccgtg gttctcgcaa atccactccg tttgcagctc aggttgcagc agagcgttgc
    13741 gctgacgccg tgaaagaata cggcatcaag aatctggaag ttatggttaa aggtccgggt
    13801 ccaggccgcg aatctactat tcgtgctctg aacgccgcag gtttccgcat cactaacatt
    13861 actgatgtga ctccgatccc tcataacggt tgtcgtccgc cgaaaaaacg tcgcgtataa
    13921 cgcctcgttt tccaggtttg ttggagaaag aaaatggcaa gatatttggg tcctaagctc
    13981 aagctgagcc gtcgtgaggg caccgactta ttccttaagt ctggcgttcg cgcgatcgat
    14041 accaagtgta aaattgaaca agctcctggc cagcacggtg cgcgtaaacc gcgtctgtct
    14101 gactatggtg tgcagttgcg tgaaaagcaa aaagttcgcc gtatctatgg tgtgctggag
    14161 cgtcagttcc gtaactacta caaagaagca gcacgtctga aaggcaacac cggtgaaaac
    14221 ctgttggctc tgctggaagg tcgtctggac aacgttgtat accgtatggg cttcggtgcc
    14281 actcgtgcag aagcacgtca gctggttagc cataaagcaa ttatggtaaa cggtcgtgtt
    14341 gttaacatcg cttcttatca ggttagtccg aatgacgttg taagcattcg tgagaaagcg
    14401 aagaagcagt ctcgcgtgaa agccgctctg gagctggctg agcagcgtga aaagccaact
    14461 tggctggaag ttgatgctgg caagatggaa ggtacgttta agcgtaagcc ggagcgttct
    14521 gatctgtctg cggacattaa cgaacacctg atcgtcgagc tttactccaa gtaaagctta
    14581 gtaccaaaga gaggacacaa tgcagggttc tgtgacagag tttctaaaac cgcgcctggt
    14641 tgatatcgag caagtgagtt cgacgcacgc caaggtgacc cttgagcctt tagagcgtgg
    14701 ctttggccat actctgggta acgcactgcg ccgtattctg ctctcatcga tgccgggttg
    14761 cgcggtgacc gaggttgaga ttgatggtgt actacatgag tacagcacca aagaaggcgt
    14821 tcaggaagat atcctggaaa tcctgctcaa cctgaaaggg ctggcggtga gagttcaggg
    14881 caaagatgaa gttattctta ccttgaataa atctggcatt ggccctgtga ctgcagccga
    14941 tatcacccac gacggtgatg tcgaaatcgt caagccgcag cacgtgatct gccacctgac
    15001 cgatgagaac gcgtctatta gcatgcgtat caaagttcag cgcggtcgtg gttatgtgcc
    15061 ggcttctacc cgaattcatt cggaagaaga tgagcgccca atcggccgtc tgctggtcga
    15121 cgcatgctac agccctgtgg agcgtattgc ctacaatgtt gaagcagcgc gtgtagaaca
    15181 gcgtaccgac ctggacaagc tggtcatcga aatggaaacc aacggcacaa tcgatcctga
    15241 agaggcgatt cgtcgtgcgg caaccattct ggctgaacaa ctggaagctt tcgttgactt
    15301 acgtgatgta cgtcagcctg aagtgaaaga agagaaacca gagttcgatc cgatcctgct
    15361 gcgccctgtt gacgatctgg aattgactgt ccgctctgct aactgcctta aagcagaagc
    15421 tatccactat atcggtgatc tggtacagcg taccgaggtt gagctcctta aaacgcctaa
    15481 ccttggtaaa aaatctctta ctgagattaa agacgtgctg gcttcccgtg gactgtctct
    15541 gggcatgcgc ttggaaaact ggccaccggc aagcatcgct gacgagtaac cggatcacag
    15601 gttaaggttt tactgagaag gataaggtca tgcgccatcg taagagtggt cgtcaactga
    15661 accgcaacag cagccatcgc caggctatgt tccgcaatat ggcaggttca ctggttcgtc
    15721 atgaaatcat caagacgact ctgcctaaag cgaaagagct gcgccgcgta gttgagccgc
    15781 tgattactct tgccaagact gatagcgttg ctaatcgtcg tctggcattc gcccgtactc
    15841 gtgataacga gatcgtggca aaactgttta acgaactggg cccgcgtttc gcgagccgtg
    15901 ccggtggtta cactcgtatt ctgaagtgtg gcttccgtgc aggcgacaac gcgccgatgg
    15961 cttacatcga gctggttgat cgttcagaga aagcagaagc tgctgcagag taatctgaag
    16021 caatgtaaaa aaacccgccc cggcgggttt ttttataccc gtagtatccc cacttatcta
    16081 caatagctgt actctttt
//
LOCUS       DAEEGA010000085        15408 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEEGA010000085 DAEEGA010000000
VERSION     DAEEGA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15408
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.085"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..1173)
                     /locus_tag="KLH46_003106"
     CDS             complement(<1..1173)
                     /locus_tag="KLH46_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBE0379706.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1372..1456)
                     /locus_tag="KLH46_003107"
     tRNA            complement(1372..1456)
                     /locus_tag="KLH46_003107"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Leu,seq:caa)
     gene            1652..2671
                     /gene="ahr"
                     /locus_tag="KLH46_003108"
     CDS             1652..2671
                     /gene="ahr"
                     /locus_tag="KLH46_003108"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBE0379707.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2800..4302
                     /locus_tag="KLH46_003109"
     CDS             2800..4302
                     /locus_tag="KLH46_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBE0379708.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRNGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="KLH46_003110"
     CDS             complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="KLH46_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBE0379709.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMVIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="KLH46_003111"
     CDS             complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="KLH46_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBE0379710.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6870..8381
                     /gene="pepA"
                     /locus_tag="KLH46_003112"
     CDS             6870..8381
                     /gene="pepA"
                     /locus_tag="KLH46_003112"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBE0379711.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8515..8958
                     /gene="holC"
                     /locus_tag="KLH46_003113"
     CDS             8515..8958
                     /gene="holC"
                     /locus_tag="KLH46_003113"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBE0379712.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8958..11813
                     /gene="valS"
                     /locus_tag="KLH46_003114"
     CDS             8958..11813
                     /gene="valS"
                     /locus_tag="KLH46_003114"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBE0379713.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11867..13063)
                     /locus_tag="KLH46_003115"
     CDS             complement(11867..13063)
                     /locus_tag="KLH46_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBE0379714.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICMVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLLFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13256..13759
                     /locus_tag="KLH46_003116"
     CDS             13256..13759
                     /locus_tag="KLH46_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0379715.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="KLH46_003117"
     CDS             complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="KLH46_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBE0379716.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14383..15396
                     /gene="argF"
                     /locus_tag="KLH46_003118"
     CDS             14383..15396
                     /gene="argF"
                     /locus_tag="KLH46_003118"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBE0379717.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3976 a         3930 c         3966 g         3536 t
ORIGIN      
        1 caacctacgc tccccaagat gttccgcctt atggatgtaa gctgctcgca cagagctacg
       61 ctcctggtga ctcatctgcc gctctaccgc atccctcgac cataatcccg actcaatcaa
      121 cgaactacaa gccatcgtcc tgaagccatg cccacaaacc tcgaccttcg tgtcataccc
      181 cattacgcgc aaagccttgt tcaccgtatt ctcactcatc ggcttacgtg gatcgtgatc
      241 gcccacaaaa atcagctctc gatttccgct catgcttttg atcttttcca aaatacttag
      301 ggcctgccgc gacaaaggta caaggtgtgg tgtccgcatc ttcgagccac gctgagaatg
      361 cttgactcct tccagttgct cacgctcacc tgggattgtc cacatagccg tttcaaaatc
      421 tatttctgac cagcgggcaa aacgcagctc gctcgaacgg atgaaaacca ataaagtgag
      481 ttcaaccgcc agtcgagtta acggtctgcc agaatagtga tctatgcgat gaagtaattc
      541 aggtatacgg ttcagttcaa gagctgcacg gtgctgtctt ttcgccgtag caaccgcacc
      601 tgcaatctct tgcgcggggt tgtagtcgat taagccgctc tgaacggcaa agcgcataat
      661 cgcggtagta cgctgctgta aacgggcggc aacttcgagc cgcccggatg actcgactgc
      721 tttaatgggt acaagaagat cccgcgtctt cagttccgca atgttccgct taccgatagc
      781 agtaaagaga ttatcctcca gacttttcaa aacacgagcg ctatgcgatg ccgaccactt
      841 ctgattgctg gcgtgccagt ctctggcgac tacttcaaac gttatcgcct cttgttcctg
      901 ttctacctta acagctttct tgttttcact gggatcgaca ccattagcta aaagcttacg
      961 agcctcatcc cggcgtgccc tggcatccgc caacgacact tcggggtatt tccccaatgc
     1021 cagcatcttc tctttgccgc caaagcgata acgtagccgc cagtatttgg agccgttagg
     1081 gtgaaccagc aaaaccatgc cttcaccgtc ggtcagctta taggcttttg cttcaggctt
     1141 agccgaacga accttcacat cactcagagc catgatgagt atcctttcaa gggttctgtg
     1201 tgggtacaaa cattatcgaa ccgggaaata cccgcagttg tacccgcatc agtaagttga
     1261 tgtagattga atcaggttga cttaggttga gtgaaaaagc gaggaaagcc ttgcggatac
     1321 tggattttag gcacaaaaaa agacgtccgt tgacgtctat tgatgttccg atggtgccga
     1381 aggccggact cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg
     1441 atttcgccac ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca
     1501 cgccaccatg caagcgccag cttgcaccca cctcgctaag tgctgaaatt ttcagcatta
     1561 cactcagcga tatgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac
     1621 ttcccaaaca acaccagaga aggccccaaa aatgtcgatg ataaaaagct atgccgcaaa
     1681 agaagcgggc ggcgaactgg aagtttatga gtacgatccc ggtgagctga agccacaaga
     1741 tgttgaagtg caggtggatt actgcgggat ttgtcattcc gatctgtcga tgatcgataa
     1801 cgaatgggga ttttcacaat atccgctggt tgccgggcat gaggtgattg ggcgcgtagt
     1861 ggcactcggg agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg
     1921 ctggacggcg cgtagctgtg gtcactgcga cgcctgtatt agcggtaatc agatcaactg
     1981 cgagcaaggt gcggtgccga caattatgaa tcgcggtggc tttgccgaga agttgcgtgc
     2041 ggactggcaa tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct
     2101 gttgtgcggc ggtatcacgg tctttaaacc actgttgatg caccatatca ctgctaccag
     2161 ccgcgttggg gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc
     2221 aatgggatgc gaggtgacgg cctttagttc taatccggcg aaagagcagg aagtactggc
     2281 gatgggtgcc gataaagtgg tgaatagccg cgatccgcag gcactgaaag ccctgtcggg
     2341 gcagtttgat ctcattatca atactgtgaa cgtcagcctc gactggcagc cttattttga
     2401 ggcgctgacc tatggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt
     2461 tccggccttt acgttaattg cgggcgaccg cagcatctct ggttctgcta ccggcacgcc
     2521 ttatgagctg cgaaagctga tgcgctttgc cgcccgcagc aaggttgcgc cgacaaccga
     2581 actgttcccg atgtcgaaaa ttaacgacgc cattcagcat gtacgcgatg gtaaagcgcg
     2641 ctaccgagta gtcctgaaag ccgacttctg atccctttgc cgggtgctgt cgcccggcac
     2701 ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt agcggtgaag agtgcacagc
     2761 cgttgcctat actccaggca ggaaactgga ggaaatctca tgagcgaacc cctgttaatt
     2821 gcccgcacgc cggacacaga actgttttta ctgccgggaa tggctaaccg taacgggctg
     2881 attactggcg caacggggac gggtaaaacc gttacgctgc aaaaactggc agagtcattg
     2941 tcggaaatcg gcgtgccggt gtttatggct gatgtgaaag gcgatctgac cggtatcgcg
     3001 caggcaggaa cggcgtcgga aaaactgctc acaaggctta aaaatatcgg cgtcaatgac
     3061 tggcaaccgc atgccaatcc ggtggtggtg tgggatatct ttggcgagaa aggccacacg
     3121 gtgcgggcga cggtttcgga tctggggccg ctgttgctgg cgcgactgtt gaatctcaac
     3181 gatgtgcaat ctggcgtgct gaatattatc ttccgcattg cggacgatca gggattgttg
     3241 ctgctcgact ttaaagattt gcgggcgatt acccagtaca tcggcgataa cgccaaatcc
     3301 ttccagaatc agtacggaaa tatcagtagc gcatcggttg gtgccatcca gcgcggatta
     3361 ctgtcgctgg aacagcaagg cgcagcacac ttctttggtg agccgatgct ggatatcaaa
     3421 gactggatgc gcaccgatgc caacggtaaa ggcgttatca atatcctcag cgccgagaaa
     3481 ctttatcaga tgccgaaact gtacgccgcc agcctgctgt ggatgctttc agagttgtat
     3541 gaacaattgc cggaagcggg cgatctggag aaaccaaaac tggtgttttt cttcgacgag
     3601 gcacatctgc tgtttaacga tgcaccgcag gtactgctgg ataagattga gcaggtgata
     3661 aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt cgcaaaaccc gtctgatatt
     3721 ccggataatg tgttcgtcca gctcggtaat cgtgttcagc acgctttgcg ggcttttacg
     3781 ccaaaagatc agaaagcagt gaagacagcg gcgcaaacca tgcgggccaa tccggcattt
     3841 gataccgaaa aggcaatcca ggaactgggg accggcgagg cgttaatctc gtttctcgat
     3901 gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga tcgcaccttg ttcgcgaatg
     3961 gggccggtga cggaagatga gcgtaatggc ctgattaatc actccccggt gtatggcaaa
     4021 tatgaagatg aggtggaccg cgagtccgcc tatgagatgc tgcaaaaagg ctttcaggcc
     4081 agtaccgagc agcaaaataa tccccccgtg aaagggaaag aggtggcggt ggatgacggt
     4141 attcttggtg gattgaagga tattttgttt ggcactaccg gaccacgcgg cgggaagaaa
     4201 gatggtgtgg tgcaaacaat ggcgaaaagc gccgctcgcc aggtgacgaa tcagattgta
     4261 cgcgggatgt tggggagttt gctggggggg agaaaaagat aagtcagacg tgatctgtgc
     4321 ccctcaccct aaccctctcc ccaaaggggc gaggggaccg ttcgaactcg tatttgactt
     4381 tgtcaacaat aataaaggga gctttcgctc ccttgttaga ttacgatttt ctcattaaca
     4441 gccacaggct gattaagaag aagctggcac ttggcaacag tgcgccgatg atcggcggga
     4501 tgccgtaaac caacgtcagc gggccgaaga tctggtccag tacgtagaag acaaaaccga
     4561 agctgatacc tgtgaccaca cgcacgccca tcggtacgct acgcagtggg ccaaagataa
     4621 acgacagcgc catcagcatc atcaccgcca cggatagcgg ctggaagatt ttgctccaca
     4681 tgttgagctg ataacgtccg gcatcctgac cgctcgactt cagatacttc acgtagttgt
     4741 gcaaaccact gatggagagt gcatccgggt ccagcgccac cacgcccagt ttgtctggcg
     4801 tgaggtcggt tttccaggtg ccgctcaccg tctgcgaacc ggtaatctgt ttcggattgg
     4861 tcagatcaga ttcatcaacc tgcgacagac gccagacttt atgttccggg tcaaacttcg
     4921 cggtagcggc ataacgtacg gattgcagac gacgattctc gttaaaggca taaatactga
     4981 tgccacctaa cacctcgtca cctttaaccc gctcaatgta gacgaagttg ttgccatctt
     5041 tcgcccataa accttgctgg gtagagagca acgagccgcc gtacatcgcc tgcgcacggt
     5101 agttacgcgc catctgttcg ccctgcggcg cgacccattc gccaatcacc atcgtcagca
     5161 agaccagtgg aatggcggtt ttcatcaccg acagcgctac ctgcatacgg gtaaaaccag
     5221 aagcctgcat caccaccagt tcgctgcgct gcgccagcat cccaagacca agcaacgccc
     5281 caagaagagc cgccatcggg aagaagatct gcacatcttt cggcacgctc agcaaggtat
     5341 acattcctgc gcctaacgcg tcgtaactcc cctgcccggc ttttttcagc tgatcgacaa
     5401 atttgataat gcccgacagc gacaccagca tgaacagcgt catcatgatg gtggtgaaaa
     5461 tagttttacc gatatagcgg tcaagtacgc caaaaggttg catcacaccg ctcctttacg
     5521 cgaaaaactg gcgcgcaggc ggcggaccgg cacggtgtcc caaaggttga gaacaatcgc
     5581 caaagccaga taaatcaggt taacggtcca catccacagt gtcgggtcca gcttaccttt
     5641 accgccgttc gatttcaggg aggtctggat caggaagaaa agtagataca gcagcatggc
     5701 tggcagcatc gacagtacgc gtccctgacg cgggttaacc acgctcaacg gtacaaccat
     5761 aagtgccatc ataaacacgg tgaataccaa cgtgatacgc cagttcagtt ctgcgcgagc
     5821 acgatcggtg tcagtgttcc acaatgtgcg catgtccatc tggtcggtat cgttcgggtc
     5881 gagcgccacc gcctggtgac caatgatcgc ctgataatcc tggaaatccg taatgcggaa
     5941 atcacgtaac agtgcagtgc cttcgaagcg cgttccctgg ttgagagtga cgacctggga
     6001 gccgtcgcgc agctgggtta aatgtccgga atcggccacc accacagaag gacgtgcatt
     6061 accttttggt cgaatttgtg cgaggaacac atctttgaaa tcgctgccgt caacgctttc
     6121 gatgaacagc accgagctgc cattagtcgc ttgctggaat tgcccttgcg ccagcgccgc
     6181 catgccaggg ttcgctttcg cttctgctaa cacttcatcc tgatgacgcg atgaccacgg
     6241 tcccgcccac atcacgttaa ccgccgcgac gattgccgtg aataccgcaa ggatcattgc
     6301 cgctttcacc agaaccgctt tgctcaagcc gcaggcatgc attaccgtaa tttcactttc
     6361 ggtatacagt ttgcccagcg tcatcagcag cccgaggaac aggcttaatg gcaggataag
     6421 ctgcgccatt tccggcacgc ccaacccgag aagggagagc accagattcg ccggaatatc
     6481 gccgtcaacc gctgcgccga ggatcctcac taacttttga cagaagaaga tcaaaagcaa
     6541 gatgaagagt atcgccagct ggcttttgag cgtctcccgc accagatatc ttatgattat
     6601 cactttaaat acgcccgtaa aaactcgtct tttgcaggat tttagcttgt ttcatggctt
     6661 aaacgtcatt tattctcttg agtcgtcgaa atcgtcgcta agataattat actcaacgga
     6721 ttcacctctc agattttgtt ctgacgtgcc aatgccgtaa taacgttaag attaacacga
     6781 agtcatcgca acagcggaca tgagttacga aagcttgcaa ttctatctgt agccaccgcc
     6841 gttgtcttta agattcagga gcgtagtgca tggagtttag tgtaaaaagc ggtagcccgg
     6901 agaaacagcg gagtgcctgc atcgtcgtgg gcgtcttcga accacgtcgc ctttctccga
     6961 ttgcagaaca gctcgataaa atcagcgatg ggtacatcag cgccctgcta cgccggggcg
     7021 aactggaagg aaaaccgggg cagacattgt tgctgcacca tgttccgaat gtactttccg
     7081 agcgaattct ccttattggt tgcggcaaag aacgtgagct ggatgagcgt cagtacaagc
     7141 aggttattca gaaaaccatt aatacgctga atgatactgg ctcgatggaa gcggtctgct
     7201 ttctgactga actgcacgtt aaaggccgta acaactactg gaaagtgcgt caggctgtcg
     7261 agacagcaaa agagacgctc tacagtttcg atcagttgaa aacgaataag agcgaaccgc
     7321 gtcgtccgct gcgtaaaatg gtgttcaacg tgccgacccg ccgtgaactg accagcggtg
     7381 agcgcgcgat ccagcacggt ctggcgattg ccgccgggat aaaagcagcg aaagatctcg
     7441 gtaatatgcc gccgaatatc tgtaacgccg cttatctcgc ctctcaggcg cgccagctgg
     7501 ctgacagcta cagcaagaat gtcatcaccc gcgttatcgg cgaacagcag atgaaagagc
     7561 tgggaatgca ctcttatctg gcggtcggtc agggttcaca aaacgaatcg ctgatgtcgg
     7621 tgattgagta caaaggcaac gcgtcggaag atgcacgccc aatcgtgctg gtgggtaaag
     7681 gtttaacctt cgactccggc ggtatctcca tcaagccttc agaaggcatg gatgagatga
     7741 agtacgatat gtgcggcgcg gcggcagttt acggcgtgat gcgtatggtc gcggagctgc
     7801 aactgccgat taacgttatt ggcgtgctgg caggctgtga aaacatgcct ggcgggcgtg
     7861 cctatcgtcc gggcgatgtg ttaaccacca tgtccggtca aaccgttgaa gtgctgaaca
     7921 ccgatgccga aggccgcctg gtactgtgcg acgtgttaac ttacgttgaa cgttttgagc
     7981 cggaagcagt gattgacgtg gcgacgctga ccggtgcctg cgtgatcgcg ctgggtcatc
     8041 atattactgg tctgatggcg aaccataatc cgctggccca tgaactgatt gccgcgtctg
     8101 aacaatccgg tgaccgcgca tggcgcttac cgctgggtga cgagtatcag gaacagctgg
     8161 agtccaattt tgccgatatg gcgaacattg gcggtcgtcc tggtggggcg attaccgcag
     8221 gttgcttcct gtcacgcttt acccgtaagt ataactgggc gcacctggat attgcaggaa
     8281 ccgcctggcg ttctggtaaa gcaaaaggcg caaccggtcg tccggtagcg ttgctggcac
     8341 agttcctgtt aaaccgcgct gggtttaacg gcgaagagta agtttgcgtt ttaccctcac
     8401 cctaaccctc tccctaaggg agagggaacc gatcgagcgc aatagaaatt cattgctcat
     8461 ctttcagggt gaggaatgca gcaatattta aatccaccac aagaagcccc atatatgaaa
     8521 aacgcaacgt tctaccttct ggacaatgac accaccgtcg atggcttaag cgccgttgag
     8581 caactggtgt gtgaaattgc cgcagaacgt tggcgcagcg gtaagcgcgt actcatcgcc
     8641 tgtgaagatg aaaagcaggc ttaccggctg gatgaagccc tgtgggcgcg tccggcagaa
     8701 agctttgttc cgcataattt agcgggagaa ggaccgcgcg gcagtgcgcc ggtggagatc
     8761 gcctggccgc aaaaacgcag cagcagcccg cgcgatatat tgattagtct gcgaacaagc
     8821 tttgcagatt ttgccaccgc tttcacagaa gtggtagact tcgttcctta tgaagattct
     8881 ctgaaacaac tggcgcgcga acgctataaa gcctaccgcg tggctggttt caacctgaat
     8941 acggcaacct ggaaataatg gaaaagacat acaacccaca agatatcgaa cagccgcttt
     9001 acgagcactg ggaaaagcag ggctacttta agcctaatgg cgatgaaagc caggaaagtt
     9061 tctgcatcat gatcccgccg ccgaacgtca ccggcagttt gcatatgggt cacgccttcc
     9121 agcaaaccat catggatacc atgatccgct atcagcgcat gcagggcaaa aacaccctgt
     9181 ggcaggtcgg tactgaccac gccgggatcg ctacccagat ggtcgttgag cgcaagattg
     9241 ccgcagaaga aggtaaaacc cgtcacgact acggccgcga agctttcatc gacaaaatct
     9301 gggaatggaa agcggaatct ggcggcacca ttacccgtca gatgcgccgt ctcggcaact
     9361 ccgtcgactg ggagcgtgaa cgcttcacca tggacgaagg cctgtccaat gcggtgaaag
     9421 aagttttcgt tcgtctgtat aaagaagacc tgatttaccg tggcaagcgc ctggtaaact
     9481 gggacccgaa actgcgtacc gctatttctg acctggaagt ggaaaaccgc gaatcgaaag
     9541 gttcgatgtg gcacatccgc tatccgctgg ctgacggcgc gaaaaccgca gacggtaaag
     9601 attatctggt ggttgcgacc actcgcccgg aaaccctgct gggcgatacc ggtgtagctg
     9661 ttaacccgga agatccgcgt tacaaagatc tgattggcaa atatgtcatt ctgccgctgg
     9721 ttaaccgtcg tattccgatc gttggcgacg aacacgccga catggaaaaa ggcaccggct
     9781 gcgtgaagat caccccggcg cacgacttta acgactatga agtcggtaaa cgtcacgccc
     9841 tgccgatgat caacatcctg acctttgacg gtgatatctg tgaaagcgcc caggtgttcg
     9901 ataccaaagg taacgaatct gacgtttatt ccagcgagat cccggcagag ttccagaaac
     9961 tcgagcgttt tgctgcacgt aaagcagtcg ttgctgcggt tgatgcactc ggcctgctgg
    10021 aagaaattaa accgcacgac ctgaccgttc cttacggcga ccgtggcggc gtggttatcg
    10081 aaccgatgct gaccgaccag tggtacgtgc gtgccgatgt actggcgaaa ccggcagttg
    10141 aagcggttga gaacggcgac attcagttcg taccgaagca gtacgaaaac atgtacttct
    10201 cctggatgcg tgatattcag gactggtgta tctctcgtca gctgtggtgg ggtcaccgta
    10261 tcccggcatg gtatgacgaa gcgggtaatg tttatgttgg ccgcaacgaa gaagaagtgc
    10321 gcaaggaaaa taacctcggt gccaacgttg ccctgcgtca ggacgaagac gttctcgaca
    10381 cctggttctc ttctgcactg tggaccttct ctacccttgg ctggccggaa aataccgacg
    10441 ccctgcgtca gttccaccca accagcgtga tggtatctgg tttcgacatc atcttcttct
    10501 ggattgcccg catgatcatg atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc
    10561 aggtgccgtt ccacactgtt tacatgaccg gtctgattcg tgacgacgaa ggtcagaaga
    10621 tgtccaaatc caagggtaac gttatcgacc cgctggatat ggttgacggt atttcgctgc
    10681 cagaactgct ggaaaaacgc accggcaata tgatgcagcc gcagctggcg gacaaaatcc
    10741 gtaagcgcac cgagaaacag ttcccgaacg gcattgagcc gcacggcacc gatgccctgc
    10801 gcttcaccct ggcggcgctg gcgtctaccg gtcgtgacat caactgggat atgaagcgtc
    10861 tggaaggcta ccgtaacttc tgtaacaagc tgtggaacgc cagccgcttt gtgctgatga
    10921 acacagaagg tcaggattgc ggcttcaacg gcggcgaaat gacgctatcg ctggcggacc
    10981 gctggattct ggcggagttc aaccagacta tcaaagcgta ccgcgaagcg ctggacagct
    11041 tccgcttcga tatcgccgca ggcattttgt atgagttcac ctggaaccag ttctgcgact
    11101 ggtatctgga gctgaccaag ccggtaatga acggtggaac agaagccgaa ctgcgcggta
    11161 ctcgccatac gctggtgact gtactggaag gtctgctgcg cctcgcgcat ccgatcattc
    11221 cgttcatcac cgaaaccatc tggcagcgtg tgaaagtact ttgcggtatc actgccgaca
    11281 ccatcatgct gcagccgttc ccgcagtatg atgcatctca ggttgatgaa gccgcactgg
    11341 ccgacaccga gtggctgaaa caggcgatcg ttgctgtacg taacatccgt gcagaaatga
    11401 acatcgcgcc gggcaaaccg ctggagctgc tgctgcgtgg ttgcagcgcg gatgcagaac
    11461 gtcgcgtaaa tgaaaaccgt ggcttcctgc aaacgctggc gcgtctggaa agcatcaccg
    11521 tgctgcctgc cgatgacaaa ggtccggttt ccgttaccaa gatcatcgac ggcgcagagc
    11581 tgctgatccc gatggctggc ctcatcaaca aagaagatga gctggcgcgc ctggcgaaag
    11641 aagtggcgaa gatcgaaggt gaaatcagcc gcatcgagaa caagctggca aatgaaggct
    11701 ttgtcgcccg cgcaccggaa gcggttatcg cgaaagagcg tgagaagctg gaaggttatg
    11761 cagaagcgaa agccaagctg attgaacagc aggctgttat cgccgcactg taatcaacac
    11821 agtgatgaaa acgaaggccg gagcatgctc cggccttttt tatctcttac agaaatggta
    11881 aagaaggcat aactccccga gaaatactgg ctaagaagcc tttatctggt tgttcttctt
    11941 tattgattaa aggaaggtca tccaaatcgc ccagcaaata cgtatttttt gcctgccagt
    12001 caatcatcca tattttcagc agtgggtaag ccagaccacc cgtaatcccg gatatcacca
    12061 ccaacgcaca catgcgataa agcataccgt ggtacgttaa agttgagcga aaacgaatac
    12121 gcccatcatt cagtgacagg ttgctcataa aatgatttcg caacgacacc gttaaataac
    12181 tggtgctaac agcaatccca aaataataaa ttaactgcgc gattatcatt ttacgttgca
    12241 tttccataaa ttgctgtaaa ttctcaatat catcatttgc atacccagaa ctatcatact
    12301 catttaatat ttgatcgaag ataatataac cagcaacagc gagaaaaagt aataaagcta
    12361 aaaatgcgag gatggcatat tttatacaat acgcagtatc taatttcacc ttgaaacgat
    12421 gtataccgaa actggtattg ctccagagaa aactcattac caggctatat aaagtaccat
    12481 taaaaatacc aatggaaaca ataccaacta ttgccatcag aaccacagat ataataacac
    12541 tacttgaact attggcatgt agcatctttg tcgagataaa gaaaacagtc cccatcccaa
    12601 tggccattaa aatcggcaag aaaaaggtta cccaccagaa ccctttcaaa gagcaattaa
    12661 aactaaatct tacgccgttg agactggtca ttaaggcctg ataacgcagt ccttttgctg
    12721 ccataaatat aagcaaaacc aacagtaaca aagtcaaaac acagccaaca agcggcatat
    12781 ctgctgacac tgtcattaag attgcgaaat agaaaaaaac aaaaacaaga caactaacaa
    12841 aaacattccc accggtaatt ccataagaaa atcgttgtcc gttaacttcc atattagcat
    12901 aaagataacg cttacatttc attaatgccc atggtaaata gatacctagc gttataatcg
    12961 ttaacaacac attcaccata caaataagaa aatatctctc acctgtacca tgaaaaacaa
    13021 aagaatggga cggaacatcc atttcattaa taacttgagc cataaaaatc ccctgaatta
    13081 aaaaatccat tgatataatt tatagataca gacagtaaaa tgttctgcca cgttacaata
    13141 ctcttatcag taataggctg gcaaaaactt tattgcttta taagacgctt gcgcacgtaa
    13201 gaagagagtg gtattaaaca aatgtataat tactttattt ttattgggtt aaactatgaa
    13261 taacattgcg ccgcaatcac ctgtaatgcg tcgcctgacg ctgcaggata atcctgctat
    13321 cgccagcgtc atccgtcagg tttacgcaga gtatggtctt accgccgata aaggctacac
    13381 cgttgccgat ccgaatctcg acgaactgta tcaagtgtac agccagcctg gccatgctta
    13441 ttgggtggtt gaatacaatg gcgaagtggt cggcggcggc gggattgcgc cattaacagg
    13501 tagtgaagcg gacatttgcg aactgcagaa aatgtacttt cttcccgcta ttcgcggtaa
    13561 agggctagcg aaaaaactgg ccttaatggc gatggagcag gcacgagaga tgggcttcaa
    13621 acgctgctat ctggaaacga ccgctttttt aaaggaagcc attgcgcttt atgagcattt
    13681 gggctttgag catatcgact atgcgcttgg ctgcacgggc catgtcgatt gtgaagtgcg
    13741 gatgctacgc gaactctaaa aaaatgcccg ctagtatgtg ctatcgggca ttttaatcaa
    13801 ttaattagtg gcgaaccccg tcatcgtctt catcgacaaa atcttcatcg tcgccatctt
    13861 cgccgttagg atcttcaaag taagtgcccc aaccgtcgta ctcaacgtca aatttctccg
    13921 ccagcgtcat cagttgttca acctgggcat cgatcagatc ggcattcaac gcgcactcgc
    13981 tgaggatgtc gcagcaaatc acgatatcac catcttcaac ttccagctct tctggatcgg
    14041 tcacttcgta accgagttta aacgcttcaa ctgccgcttt ttccagagtt tccagatcgt
    14101 ctgcggaaag atggtgttcg atggtgtaca gcgcgtccgg atcgctgcca tcttccagta
    14161 attcttcaat aatcaaacgt gtttcttcac gctgttcttc cagttgttcc gggtttgcca
    14221 tggctcattc ctcttaatgt cctgccgata ctcttattgt cacacaccac tgacattgcc
    14281 tccacctttg tgacaaagat ttatgcttta gacttgcaaa tgaataataa tacacataaa
    14341 ttgaatttta attcattgag gcgttagcca caggagggat ctatgtccgg gttttatcat
    14401 aagcatttcc tgaaattact cgatttcacg ccagctgaac tcaacagcct gctgcagtta
    14461 gccgcgaagc tgaaagctga taagaaaagc ggtaaagaag aagccaaact cactggtaaa
    14521 aacatcgcgc tcatcttcga aaaagactcg actcgtaccc gatgctcttt cgaagttgcc
    14581 gcatatgacc agggggctcg cgttacttat cttggcccaa gcggtagtca gattggtcat
    14641 aaagagtcga ttaaagacac tgcccgcgtg cttggtcgca tgtatgacgg tattcagtat
    14701 cgcggctatg gtcaggagat tgtcgaaaca ctggcggaat acgctggcgt gccggtatgg
    14761 aacggcctga ccaatgagtt tcaccccacg cagttgctgg cggatctcct caccatgcag
    14821 gagcatttac ccggtaaagc attcaacgaa atgacgctgg tctatgcagg tgacgcacgt
    14881 aacaacatgg gcaattcgat gctcgaagct gcggcgctta ccggtctgga tttgcgtctg
    14941 gtcgcgccac aggcatgctg gccagaagct gcgctggtta cggaatgccg cgccctggca
    15001 caacaaaatg gcgggaatat tacgctgacg gaagatgtag ctaaaggagt tgaaggtgct
    15061 gactttatct ataccgatgt atgggtgtcg atgggtgaag caaaagaaaa atgggctgag
    15121 cgcattgcat tactgcgtga ttatcaggtg aacagcaaga tgatgcagtt gaccggtaac
    15181 ccggaggtca aattcctcca ttgcctgccc gcgtttcatg acgaccaaac gacgcttggc
    15241 aaaaaaatgg cggaagagtt tggcctacat ggcggaatgg aagtgactga tgaggtcttc
    15301 gaatctgccg ccagcattgt atttgatcag gcggaaaacc gcatgcatac catcaaagcg
    15361 gtgatggtcg cgacgctcag taaattaaat aattaatatc tctataaa
//
LOCUS       DAEEGA010000086        15344 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEEGA010000086 DAEEGA010000000
VERSION     DAEEGA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.086"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="KLH46_003119"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="KLH46_003119"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBE0379718.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNASKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="KLH46_003120"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="KLH46_003120"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBE0379719.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="KLH46_003121"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="KLH46_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HBE0379720.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="KLH46_003122"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="KLH46_003122"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBE0379721.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="KLH46_003123"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="KLH46_003123"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBE0379722.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="KLH46_003124"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="KLH46_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBE0379723.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="KLH46_003125"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="KLH46_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HBE0379724.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="KLH46_003126"
     CDS             complement(8064..9206)
                     /locus_tag="KLH46_003126"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HBE0379725.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="KLH46_003127"
     CDS             complement(9215..10228)
                     /locus_tag="KLH46_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HBE0379726.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="KLH46_003128"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="KLH46_003128"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBE0379727.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="KLH46_003129"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="KLH46_003129"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HBE0379728.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="KLH46_003130"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="KLH46_003130"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBE0379729.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="KLH46_003131"
     CDS             complement(12826..13950)
                     /locus_tag="KLH46_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBE0379730.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="KLH46_003132"
     CDS             complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="KLH46_003132"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBE0379731.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4379 a         3445 c         3146 g         4374 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttgg aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctgcc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgctaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aacatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atagcaacca tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat
    14761 accttgatga gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt
    14821 atgccgtaga gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa
    14881 agcagcacat cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta
    14941 gttaataaca tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta
    15001 tccacaagta actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct
    15061 accgccccag gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt
    15121 gtatcacttc tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca
    15181 ataaagaaat caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga
    15241 ttccttcctg cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag
    15301 atgttcagca agaattggcg ggtaaattgc tatttaagtc aaaa
//
LOCUS       DAEEGA010000087        15098 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEEGA010000087 DAEEGA010000000
VERSION     DAEEGA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15098
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.087"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="KLH46_003133"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="KLH46_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBE0379732.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="KLH46_003134"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="KLH46_003134"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBE0379733.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRYLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="KLH46_003135"
     CDS             1531..2478
                     /locus_tag="KLH46_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBE0379734.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="KLH46_003136"
     CDS             2548..3354
                     /locus_tag="KLH46_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE0379735.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="KLH46_003137"
                     /pseudo
     CDS             3364..3771
                     /locus_tag="KLH46_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3771..4265
                     /locus_tag="KLH46_003138"
     CDS             3771..4265
                     /locus_tag="KLH46_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HBE0379736.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="KLH46_003139"
     CDS             4331..5128
                     /locus_tag="KLH46_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBE0379737.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="KLH46_003140"
     CDS             5139..5927
                     /locus_tag="KLH46_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBE0379738.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="KLH46_003141"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="KLH46_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HBE0379739.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="KLH46_003142"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="KLH46_003142"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBE0379740.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="KLH46_003143"
     CDS             complement(8370..10001)
                     /locus_tag="KLH46_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBE0379741.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFAAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="KLH46_003144"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="KLH46_003144"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBE0379742.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="KLH46_003145"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="KLH46_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBE0379743.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELSQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3590 a         3982 c         3937 g         3589 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatagcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggagaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtagaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca tggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggcgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct agtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc gcacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gcggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gtacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcggcga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcagg ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattaa gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatagt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gcggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 ctcaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg tcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttca
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaa
//
LOCUS       DAEEGA010000088        15051 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEEGA010000088 DAEEGA010000000
VERSION     DAEEGA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15051
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.088"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..177)
                     /locus_tag="KLH46_003146"
     CDS             complement(<1..177)
                     /locus_tag="KLH46_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379744.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQFSLCLKMEG
                     EEQLCDCVNELKHGL"
     gene            complement(593..1534)
                     /gene="fabY"
                     /locus_tag="KLH46_003147"
                     /gene_synonym="yiiD"
     CDS             complement(593..1534)
                     /gene="fabY"
                     /locus_tag="KLH46_003147"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBE0379745.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1579..2016)
                     /gene="dtd"
                     /locus_tag="KLH46_003148"
     CDS             complement(1579..2016)
                     /gene="dtd"
                     /locus_tag="KLH46_003148"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBE0379746.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2013..2885)
                     /locus_tag="KLH46_003149"
     CDS             complement(2013..2885)
                     /locus_tag="KLH46_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBE0379747.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2879..3478)
                     /gene="yihX"
                     /locus_tag="KLH46_003150"
     CDS             complement(2879..3478)
                     /gene="yihX"
                     /locus_tag="KLH46_003150"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBE0379748.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3577..4362)
                     /locus_tag="KLH46_003151"
     CDS             complement(3577..4362)
                     /locus_tag="KLH46_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE0379749.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4396..5292)
                     /gene="yihV"
                     /locus_tag="KLH46_003152"
     CDS             complement(4396..5292)
                     /gene="yihV"
                     /locus_tag="KLH46_003152"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBE0379750.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGLPDCDQTRSFLSLFV"
     gene            5460..6356
                     /gene="yihU"
                     /locus_tag="KLH46_003153"
     CDS             5460..6356
                     /gene="yihU"
                     /locus_tag="KLH46_003153"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBE0379751.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGHGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6380..7258
                     /gene="yihT"
                     /locus_tag="KLH46_003154"
     CDS             6380..7258
                     /gene="yihT"
                     /locus_tag="KLH46_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HBE0379752.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7275..8516
                     /gene="yihS"
                     /locus_tag="KLH46_003155"
     CDS             7275..8516
                     /gene="yihS"
                     /locus_tag="KLH46_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBE0379753.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8654..9556
                     /locus_tag="KLH46_003156"
                     /pseudo
     CDS             8654..9556
                     /locus_tag="KLH46_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
     gene            9755..11791
                     /gene="yihQ"
                     /locus_tag="KLH46_003157"
     CDS             9755..11791
                     /gene="yihQ"
                     /locus_tag="KLH46_003157"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HBE0379754.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11837..13211
                     /locus_tag="KLH46_003158"
                     /pseudo
     CDS             11837..13211
                     /locus_tag="KLH46_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13254..14657
                     /locus_tag="KLH46_003159"
     CDS             13254..14657
                     /locus_tag="KLH46_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0379755.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14725..>15051
                     /gene="ompL"
                     /locus_tag="KLH46_003160"
     CDS             14725..>15051
                     /gene="ompL"
                     /locus_tag="KLH46_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBE0379756.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3700 a         3875 c         3743 g         3733 t
ORIGIN      
        1 caaaccatgc ttcagttcgt taacacaatc gcacagttgc tcctcacctt ccattttaag
       61 gcaaagtgaa aattgattta atagcgtttg cttcggtgat ttattactca ttagttgttt
      121 ttctaaatta acaacagaat aactttcttg tttaatgaga ttaatattgg caaccattat
      181 agcctccttc ccacggttta agagcaaaca tacaacataa tgattcataa catacagagc
      241 agtaaatgaa tgatactgac aatgtatgcc agcccattat ttttgcataa tatttgtgag
      301 ctatagattt taaaaatatt tatgaaaaaa gtatggcacc agagggggtt ttgactatca
      361 aaaatcgctc aaacctgaac taatatgatg agttaagcat tatctgcaat aattgccact
      421 cagctctaca aataatattt aaatgctcct gaacagtgat gtagcaagac acgtatggtg
      481 ccaacgaaga ataaaaattt ataaagttct tattcaatat aaccggaata gtcagggact
      541 gatgtaaatg accggaatga atgagagagg cagcaccgcc cctctccata agctactctt
      601 cttcgttccc gccctcttca tacgggccaa atggcttcgc gggcagaacg atatacgtgc
      661 cttcaaacac tgcacccggc gtctcgtcgc caaagatttc gacctgcatc tgcacccgtg
      721 cttttcgtcc gcgcgccaga cggtcgagat cgccgcttaa ggcaccaagg tcggctaccg
      781 catggggttt accgctaatc ggtttgctgt agcggatatg cgcatccgcc agaataatcg
      841 ttccgccgag gtggcgttca cgcagcatca gccagataag cccccaaccg gtgagcgtcg
      901 ccagcgagaa taaactcccg gcaaacagcg tatggtgcgg attctgattg ccggtttccg
      961 gcatggtggt gataaatttt tgcccggtat attgttgaat acgcacgccc attttttcac
     1021 taagcgggat atgttcgtac cacgcctgtt gcagctgcgc gcaccagtcg ccgcgatgca
     1081 aaatatcatc cagagtggcg acgggcttaa tcatcaaaaa atggcgaatc ggcgtggtgg
     1141 ttggcgtggt gatttctccc tgattaacaa accccagctt ggcgaaaaac tccaccgcgt
     1201 cttcacgggc gctacaggtc acgcgcttaa cgccttcctg acgcgccacc gactccaggg
     1261 tcatcgccat cagcgttcct aaccctttgt cctgcacgtc cgggtgaacg accataaagc
     1321 gaatggacgc ttcattgtcg gcattaatat acaatcggcc taccgccacc agattaccct
     1381 gctcgtcgac gaccatctgg tgatgcgcca tcgcatccca cgcgtcgcgt tccgaacctt
     1441 ttggttgatg caggggctta cgcaacattt cccagcgaaa ctgatagtaa cgctctaact
     1501 cttcttctgt ttgtggaacc tgaaggtgat acatagcgat actctctctt gtttcccgtg
     1561 acaaccctgg aagctggctc atacctgcaa ccagaatgtc acggggccat cattgaccag
     1621 cgatacctgc atatcggcag cgaagcgtcc tgtttgcgtg ttcatctctt gctggcggca
     1681 gcgttcgacg aaatagtcat ataacgcctc tgcgcgatcc ggtgatgcgc ctttggagaa
     1741 acttgggcgc atcccccgtt cggtatctgc ggcgagggta aactgggaaa ccaccagtac
     1801 actgccgccc gcctgttgca cgttgagatt catcttgcct tcggcatcgc taaagatgcg
     1861 gtagccgagc acacgctcgc acagacggtt tgctttctgt tcgtcgtcat ccttttcgac
     1921 acccaataac accaaaagtc ccgcgccaat ttcgcccgtc acttctccct ccacggtgac
     1981 gctggcacgg gttacgcgtt gaattaatgc aatcatggtt cgtcgtcttc ttcttgttca
     2041 gctgcttgtt ttagtttgcg gtattccccg agagtgacag taatttccgc gccaagcaag
     2101 acgatacacc atgtccagta gacccaaaca aagagaatgg ggatcaccgc cagcacaccg
     2161 taaatgagct gatatgacgg gaacatggtg atataaagcg cgaaaccttt ctttcctgct
     2221 tcgaacagga gtgcggcgac aaacgcgccg acaatcgcgt cgcggttagg tacgcggatg
     2281 gtaggaacaa tgctgtacag caaccagaag gagatccacg acaacagcaa cggaaaaata
     2341 cgcagcacgt tgtcgatgac agtattgaga tcgctcgccc agcgcaggga gagcaaatag
     2401 gaactgatcg ccagactggc ccctgccagc agcggcccca gcgttaaaat catccagtac
     2461 acggcgaacg agtaaatttt tggtcgcgct cgtttactgc gccagatggt attcaacgcg
     2521 ctatcgatgg agtacatcaa caataacgcg gtgacgatca gcccgcacgc cccaacggcg
     2581 gtcatcttgt tggaattagc aacaaattgt tcgatatacc gctgaataac atcgccagta
     2641 gcaggcagaa agttggcaaa aataaagtga cgtaactgaa tgctgacgtc ggaaaacatg
     2701 ggaaaagcgg cgaaaagcgc aaaaacaacg gcaaccagcg gcactaatga gagcaacgac
     2761 acataggcaa ggttacctgc cagggttgtc atgttgtcct catcaatacg ttgccagagt
     2821 agttttagcc aggcccatag tggacgggta cggtgcctgg ctttgtcctg aatggttttt
     2881 agcataacac cttcgcgaaa tagtccggga tggtggtttt atctttcacc agaatactgg
     2941 taatgcctag ctgattagct ccttctatat tatcggcgtt atcgtcgaaa aagaccgtat
     3001 cgctgggtga aaaaccttcc gcctgcaaaa catgctggta aattcgtgct tcaggtttgc
     3061 gcatccccag atcttgcgac agatagatat ggtcagcagc atcacgaatt tccgggtatt
     3121 cttccggcca gaaggtggta tgcaggcggt tggtattgga aagcaccacc acgcgatgcc
     3181 cctgctcacg cagtttatgc atgatggcga tcacttccgg gcgcagcgca acaaacaccg
     3241 cctgccagcc gtgagagaac tgctcgtagc ttagcggtag agccatctca tgacacagcg
     3301 cctctgcgaa cgcttcatcg ctaatttccc cacgctcatg ctgatgaaac gcctccccca
     3361 tatgaaaact cttcttaagc gatgccagcg gaatacgcgt taaatcgctc caggctccca
     3421 gcacacggtt aaagtcgata tcgacaatca cattacctaa atcaaagata tagagcattt
     3481 ttgcctcctt tcgcgccaca agaaattaac tgtagcggga aaggatgggt ttggctatgt
     3541 ggcaaggtat gaagccaggt taacggtcac aaaaatctac gcgtcttcct ggggaagaat
     3601 gatttcaatt tggctgtctt gtaagcgtga ttttagcgca gcgggcggca gctcgtcggt
     3661 aaagagcgca gtgacctgtg ccacgttacc aatttcaacc gccgccgaag catgatactt
     3721 agtgtgatcg gcgaccagca gaatatttct cgcgtgcgcc atcatcgttt tcaccacgtt
     3781 agcttcgttt acatcaaact ccatcaacgc gccatcgctc tcaatcgccc caacgcttgt
     3841 taccagataa tcagcacgaa aatcagccac aaaggacgcc gctgaagggc caatgatccc
     3901 gctattatga gagcgcaacg taccgccggg caccatcact tcaaagcgcg ggttgtggta
     3961 aagaatatgc gccacacgca ggctgttggt gattatccgc aaatgattat ggttaagtaa
     4021 cgcccgggca acatgctcaa cagtcgtacc aatggtgata aatattgttg aaccatcagg
     4081 aatatagtct gccaccgctt cggcaatcgc tttttttttc tcggtttgcg aaacctcacg
     4141 ctgctcgaac gccgtattaa cgacgctgga agcccgaccc gcgccaccgt gatggcgcgt
     4201 aatcaggcct tgctcgctta atttacgaat atcccggcgg accgtctgcg tggagacatc
     4261 cagcagattt gccagctcat caatattcat atacccacgc tcggcgatca gcatgaggag
     4321 ttggtcgtgc cgcgggttac cggttagttc ggtaaggctc atgaaaattc ctcgaaaaac
     4381 catcaccctg gcattttata caaaaagtga caaaaaagat cgggtttgat cacagtcagg
     4441 gagcccggct cgtccaccgg gacgtgtgca ttttaacgcc gctacaccgc tggcgaagcg
     4501 gactgactcc gctaaatccc cacttgttgc cagcgccacc gccaaagcgc cgtgaaaaac
     4561 atcacccgca cctgtggtat ctaccacatc aactttgaag gccggttgat gctgacgccc
     4621 accattttcc agccagtcgc agcctgcgct accctgggtc acatagacat gaccatttgt
     4681 gagcgtttgt gcctgtttta gcgcactggc catctctttc acgcccgtta agcgcgccag
     4741 acccggttct gaaaaggccg cgtgatcgct taatgccacc agctcactga tatcctgcgg
     4801 cgtaatgtcc ccgtccagaa cggtcatcac acccgcctga cgggccaggg tgaaggcttt
     4861 tttagcgccg tcgtgccagc gtacatctgc cagcacaaca tcccactgag agaaatcaat
     4921 ttcctccaac cactctgcgt caggcagcag atccgggctg gggtagttaa tgattatccg
     4981 ctcgcctttg gtatccacca tgatagcgga ttgcgaagat ttcgcctggt tataccgttt
     5041 ggtgtaacgg gtgttaacgc cccaggattc cagctctgcc agcaggctgt tgcccgcgtc
     5101 gtcatcacct acgcgaccaa taaaatcgac ctgcgccccc agccttgccg ccgcaaccgc
     5161 tgccgtcgcc gctggcccgc caccaacttc cgtataattt ctcgccacgt atttaccgct
     5221 ctccgtcggt aacccttcca cgtaatagat gcgatccatc acggttatac ctacacaagc
     5281 aacacgaatc atggtcattc cttaaacatt ttgggatgcg ttcattttaa tttcaccaat
     5341 tgtttaaaaa gtgacggctg tcaatttttt gacgataaat tacaaattcg atcaaaaaca
     5401 gacaagcaaa gcatgtcata aatgacaaaa agtgacatca ctgtattcag gagagggtta
     5461 tggcagcaat cgcgtttatc ggtttaggac aaatgggttc gccaatggcg agcaatttat
     5521 tgcagcaagg gcatcaactt cgcgtctttg atgtgaacgc cgaagctgtg cggcatctgg
     5581 tagacaaagg tgcgactccc gccgccaacc cggcgcaggc cgctaaagat gccgaattta
     5641 tcattaccat gttgccgaat ggcgatctgg tgcgcaacgt gttgttcggt gaaaacggcg
     5701 tttgcgaagg cttatctacc gatgcgctgg tcattgatat gtccaccatc catccgctgc
     5761 aaaccgataa attgattgcc gatatgcaag ccaaaggctt cagcatgatg gatgttccgg
     5821 taggccgtac ttctgcaaat gccattaccg gtactctgtt actgctggct ggcggcaccg
     5881 ctgaacaagt tgaacgtgcc acaccgatcc tgatggcgat gggcagtgag ttgatcaacg
     5941 ctggcggtcc gggcatgggg atccgcgtta agctcatcaa caactacatg agcatcgcgc
     6001 tcaatgcgct ttcggcagaa gccgccgttt tgtgcgaagc cctgaatctt cccttcgatg
     6061 ttgccgtcaa agtgatgagc ggtaccgccg ccggtaaagg tcacttcacc acttcctggc
     6121 caaacaaagt cctcagcgga gatctttctc ccgccttcat gatcgatctt gcccataagg
     6181 atcttggcat cgcccttgat gtcgccaacc agctgcatgt gccaatgccg ctgggggccg
     6241 cctcacggga ggtttatagc caggcgcgcg cagcgggtca cggtcgccag gactggtccg
     6301 ccattctgga acaggtccgt gtcagtgccg ggatgactgc caaagtaaaa atgtaacgac
     6361 tggataaagg aataaatgaa tgaataagta caccatcaac gacattacgc gcgcatcggg
     6421 cggttttgcc atgctggcgg tcgatcagcg cgaagccatg cgcatgatgt ttgccgcggc
     6481 tggggcaccc gctccggtag ccgatagcgt tttaactgat ttcaaagtta acgctgcaaa
     6541 ggccctctcg ccttatgcct cggcgattct ggtagatcaa caattctgct accgccaggt
     6601 ggttgagcaa aacgcgattg ccaaaagttg cgccatgatt gtcgccgccg atgagttcat
     6661 tcctggcaac ggtattccgg tcgatagcgt ggttattgac cgcaaaatca atccgctaca
     6721 gatcaaacaa gacggtggca aagccttaaa actgctggtg ctgtggcgta gtgatgaaga
     6781 tgcgcagcaa cgtctggata tggtgaaaga gttcaacgaa ctgtgccact cacacggtct
     6841 ggtaagcatc attgagccag tcgtccgccc accgcgtcgt ggcgataaat tcgatcgcga
     6901 acaagcgatc atcgatgccg ccaaagagct gggcgacagt ggcgctgacc tctacaaagt
     6961 tgaaatgccg ctttatggca aaggtccgca acaagagctt ctctgtgctt cacaacgtct
     7021 gaatgaccat atcaatatgc catgggtgat cctctcttcc agtgtcgacg aaaaactgtt
     7081 cccgcgtgcc gtacgcgtgg caatgacggc gggcgcatcg ggattcctgg caggccgtgc
     7141 agtctgggca tcggtcgtcg gtttaccaga caacgagctg atgctccgtg acgtttgcgc
     7201 accgaaatta caacaacttg gcgatatcgt cgacgaaatg atggctaaac gccgctaaga
     7261 tgaggataaa aggaatgaaa tggtttaaca ccctaagcca caaccgttgg ctggaacagg
     7321 aaaccgaccg catctttgat tttggtaaaa attccgtagt gccgactggt tttggctggt
     7381 taggcaataa agggcaaatc aaagaagaga tgggcaccca tctgtggatc acagctcgta
     7441 tgttgcacgt ttattccgtt gctgcggcga tgggtcgacc tggcgcttac gcgttggttg
     7501 atcacggtat taaagccatg aacggcgcac tgcgtgataa aaaatatggc ggctggtatg
     7561 cctgcgtgaa tgacgagggc gtggtggatg cctccaaaca gggctatcaa cacttctttg
     7621 ctctgctggg tgccgccagc gccgtcacaa cgggtcaccc ggaagcgcgc aagctgctcg
     7681 attacaccat tgagattatc gagaaatatt tctggagcga agaagagcag atgtgcctgg
     7741 agtcctggga cgaagccttc agcaaaaccg aagagtaccg cggcggcaat gccaatatgc
     7801 acgcggtgga agctttcttg attgtttatg acgtcactca cgacaaaaaa tggctggatc
     7861 gcgcgattcg cgtggcttcc gtgattatcc acgacgtcgc cagaaataat cattatcgcg
     7921 ttaacgaaca cttcgatacc cagtggaatc cgctgccgga ttacaacaaa gataacccgg
     7981 cgcatcgctt ccgcgcgttc ggaggtacac caggccactg gatcgaatgg ggccgtttaa
     8041 tgctgcacat ccatgctgcc ctggaagccc gttgcgaaca accaccagca tggctgctag
     8101 aagatgccaa aggtctgttt aacgccaccg tgcgcgatgc ctgggcaccc gatggagcgg
     8161 acgggattgt ttataccgtt gactgggaag gaaaaccggt ggtccgcgaa cgtgtacgtt
     8221 ggcctatcgt cgaagcaatg ggtacggcct acgcgctcta caccgtcact ggcgatcgcc
     8281 agtatgaaac ctggtatcaa acatggtggg agtactgcat taagtacctg atggactatg
     8341 aaaatggttc ctggtggcag gagctggatg cggacaataa ggtcatcacc aaagtctggg
     8401 acggcaaaca ggatatttat cacctgctgc attgcctggt gatcccgcgt atcccgttag
     8461 cgcccggcat ggctccagcg gttgcagcgg gtctgctgga tattaatgcg aaataactga
     8521 tctgaaaata gggtgcgggt tttcccctca ccctaaccct ctccccaaag gggcgagggg
     8581 accgtccggt gcagtttttg ggtttgacat cagcctcacg ggaggctgta tgtcgttaat
     8641 taaggtgcct tgtatgcaaa tcaccaatat gcattgcagt ggacagaccg tttcacttgc
     8701 cgcgggggat tatcacgcga ccattgtgac cgtcggtgct ggtctggctg aattaacctt
     8761 tcagggttgt catttagtca tcccgcacaa acccgaagag atgccgctgg cgcatctggg
     8821 taaagtgctt attccgtggc cgaaccggat cgccaacggc tgttaccgtt atcaggggca
     8881 ggaatatcag ttacccatta atgaacatag ttcaaaagcc gctattcacg ggctgcttgc
     8941 ctggcgagac tggcaaatta gtgaattaac caccaccagc gttaccctga cagcttttct
     9001 gccgcccagt tacggctatc cgtttatgct ggcttcacaa gttgtttatt cgctcaacgc
     9061 acacacgggt ctttccgttg agatcgcaag ccagaatatc ggcactgtcg cagcgcctta
     9121 tggcgtgggt attcatcctt atttaacctg caacttaaca tctgttgatg aatatctgtt
     9181 ccagcttccg gcaaactagg tttatgccgt cgatgaacac gtaaatccga caacgcttca
     9241 ccacgttgat gagctggatt taaatttcac gcaggcgaaa aaaatagccg cgacaaaaat
     9301 cgaccacacc tttaaaaccg ctaatgattt gtgggaaatg accattaccc atccacagca
     9361 ggcgctttcg gtaagccttt gttccgatca accctgggtg caggtttata gcggtgaaaa
     9421 attacagcgt cagggactgg cagttgaacc tatgagttgc ccgcctaacg cctttaattc
     9481 aggtatcgac ttattattac tggaaccagg aaaaccgcac cgcctgtttt ttaatattta
     9541 cggacagaga aaataacttg tattgattag cagtcaaaat cattgaagaa catttcactc
     9601 gctaaaggca acattatgta tcttcaattt attaagttaa aaacagctca gcttctgtaa
     9661 agtttttata tatcagtttt atgagaatca ttttacataa gcctgtaata acaggaaggc
     9721 aacaatctcg ttctcaactc tatggagatt agttatggat acgccacgtc cacagttatt
     9781 agattttcaa tttcatcaga ataacgacag ttttacccta cattttcaac aacgtcttat
     9841 tttaacccat aacaaagata atccttgttt atggattggc tcaggtatag cggatatcga
     9901 tatgttccgc ggtaatttca gcattaaaga taaactacag gagaaaattg cgcttaccga
     9961 cgccatcgtc agccagtcac cggatggttg gttaattcat ttcagccgtg gttctgacat
    10021 tagcgccacg ctgaatatct ctgccgacga tcagggccgt ttattgctgg aactacaaaa
    10081 cgacaacctt aaccacaacc gtatctggct gcgccttgcc gctcaaccag aggaccatat
    10141 ctacggctgc ggcgaacagt tttcctactt cgatctgcgc ggcaaaccgt tcccgctatg
    10201 gaccagtgaa caaggcgttg gtcgcaacaa acaaacctat gtcacctggc aggccgactg
    10261 caaagaaaat gcgggcggcg actattactg gactttcttc ccacagccta cgtttgtcag
    10321 cacgcagaag tattactgcc atgttgataa cagttgctac atgaacttcg actttagcgc
    10381 cccggaatac catgaactgg cgctgtggga agacaaagct acgctacgtt ttgaatgtgc
    10441 tgacacatac atctccctgc tggaaaaatt aactgccctg ctgggacgcc agccagaact
    10501 gcccgactgg atttatgacg gggtaacgct cggcattcag ggcgggacgg aagtgtgcca
    10561 gaagaaactg gacaccatgc gtaacgcggg cgtgaaggtt aacggcatct gggcgcagga
    10621 ctggtctggg atccgtataa cctcttttgg caagcgcgtg atgtggaact ggaagtggaa
    10681 cagcgaaaac tacccgcaac tggattcacg cattaagcag tggtatcagg agggcgtgca
    10741 gttcctggcc tatatcaacc cgtatgttgc cagcgataaa gatctctgcg aagaagcggc
    10801 acaacacggc tatctagcaa aagatgcctc tggcggtgac tatctggtgg agtttggcga
    10861 gttttacggc ggcgttgtcg atctcactaa tccagaagcc tacgcctggt tcaaggaagt
    10921 gatcaaaaag aacatgattg aactcggctg cggcggctgg atggctgact tcggcgagta
    10981 tctgcccacc gacacgtacc tgcataacgg cgtcagtgcc gaaattatgc ataacgcctg
    11041 gcctgcgctg tgggcgaagt gtaactacga agcccttgaa gaaacgggca agctcggcga
    11101 gatcctcttc tttatgcgcg ccggttctac cggtagccag aaatactcca ccatgatgtg
    11161 ggcgggcgac cagaacgtgg actggagtct cgacgatggc ctggcgtccg tagtcccggc
    11221 ggcgctgtcg ctggcaatga ccggacatgg tctgcatcac agcgacattg gcggttacac
    11281 caccctgttt gagatgaagc gcagcaaaga gctgctgctg cgctggtgcg atttcagcgc
    11341 cttcacgccg atgatgcgca cccacgaagg taaccgtcct ggcgacaact ggcagtttga
    11401 cggcgatgcg gaaactatcg cccattttgc ccgtatgact accgttttca ccaccctgaa
    11461 accttatctg aaagaggccg tcgcgctgaa tgcgaagtcc ggtctgccgg tcatgcgccc
    11521 gctgttcctg cattacgaag acgatgcaca tacctattcc ctgaaatatc agtacctgtt
    11581 aggccgcgac attctggtcg ctccggtaca cgaagaaggt cgcagcgact ggacgctcta
    11641 tctgccggag gataactggg tacatgcctg gacgggtgaa gcgttccggg gcggggaagt
    11701 taccgttaat gcgcccatcg gcaagccgcc ggtcttttat cgcgccgata gcgaatgggc
    11761 ggcactgttc gcgtcgttaa acagcatcta atcacatccg cccgtgggga aactcatggg
    11821 caaagggaga acatcgatga gtcacatcac aacggaagat ccagcaactt tacgcctgcc
    11881 ctttaaagag aaactctctt acggtattgg cgacctggcc tctaacatcc tgctggatat
    11941 cggtacgctt tatcttttga agttttatac cgacgttctg gggctgccag gcacctatgg
    12001 cggcattatc tttttgattt caaaattctt tactgcgttt accgatatgg gtaccggcat
    12061 tatgttggat tcccgacgca agatcggtcc aaaaggtaag ttccgtcctt ttattctgta
    12121 tgcgtcattc ccggtcacct tactggcgat cgccaacttt gtcggcacac cgtttgatgt
    12181 caccggtaaa acggtgatgg ccactattct gtttatgctt tacggactgt ttttcagcat
    12241 gatgaactgc tcctacggcg caatggtccc ggcaatcacc aaaaacccca acgaacgcgc
    12301 ctcactggcg gcatggcgtc agggcggcgc tacgctgggc ctgctgctgt gcacggtggg
    12361 attcgtgcca gttatgaatc ttatcgaagg taatcagaaa cttggctata tcttcgccgc
    12421 cacgctgttt tcactgttcg gcctgctgtt tatgtggatc tgctactcag gcgtgaaaga
    12481 gcgttatgtc gaaacccaac caaccaatcc ggcgcaaaag cctggcctgt tgcaatcttt
    12541 ccgcgcaatt gccggtaacc gcccactgtt cattctgtgt attgccaacc tctgcacctt
    12601 aggggcgttt aacgtcaagc tcgccattca ggtctattac acccagtacg tacttaacga
    12661 tcccatcctg ttgtcgtata tgggattttt cagcatgggc tgtattttca tcggcgtgtt
    12721 cctgatgcct ggcgcagtca gacgttttgg taagaagaag gtctatatcg gcggcctgct
    12781 gatttgggtg ctgggcgatc tgctcaacta tttcttcggc ggcggctcgg tcagctttgt
    12841 ggcgttctcc tgcctggcgt tcttcggctc agcgcctgaa ctgggcgctg gtttccgaca
    12901 ccgtcgagta cggcgagtgg cgcaccggcg tgcgttcgga aggcacggtc tacaccggct
    12961 tcaccttctt tcgcaaagtg tctcaggcgc tggctggttt cttccccggc tggatgctga
    13021 cgcaaattgg ctatgtgccg aacgtcgcac aggctgacca caccattgaa gggttacgcc
    13081 agttgatctt catctaccca agcgcactgg cggtagtcac cattgtggcg atgggttgct
    13141 tctacagcct gaacgagaag atgtatgtcc gcattgtgga agagatagaa gcccgtaaac
    13201 gcacggcgta ataataataa acgacgccct gcggggcgtt ataaggagtg attatgtctg
    13261 accataatcc actgacatta aaactgaatc tgcgggaaaa aatcgcctat ggtatgggcg
    13321 acgtcggttc gaatttaatg ctctgcatcg gtactctgta tctcctcaaa ctttataccg
    13381 atgaattagg gatgcctgct tactatggcg ggattatctt tctggtcgcg aagtttttta
    13441 ccgcgtttac cgatatgctc accggatttt tactcgactc gcggaaaaat attgggccta
    13501 aaggcaaatt tcgtcccttt attttatatg cagcagttcc ggcggcgtta attgcaacgc
    13561 tccagtttat cgccaccacc ttttgtttgc cggttaaaac gacgattgcc accacgctgt
    13621 ttatgatgtt tggactttca tacagtctga tgaactgctc gtatggtgcg atgatcccgg
    13681 caattaccaa aaacccgaat gagcgcgcgc agctcgcggc ttaccgccag ggtggtgcaa
    13741 ccatagggct attgatttgt accgtggcgt ttattccgtt gcagtcgctt ttttctgact
    13801 caaccgtcgg ttatgcctgt gcggcactta tgttctccat tggcggcttt atttttatga
    13861 tgctgtgcta cagaggcgtc aaagagcatt atgtggacac agcgccaacc ggacataaag
    13921 ccagtattct caaatctttt tgcgcgatat ttcgtaatcc accattgctg gttttatgca
    13981 ttgctaacct gtgtactctg gcggcattta atatcaaact ggcgattcag gtctattaca
    14041 cccagtatgt gctgaatgat attaatttat tgtcgtggat gggattcttc agtatgggat
    14101 gcatcctcgt cggcgtatta ctggtgccag taactgtaaa atgttttggt aaaaaacagg
    14161 tttatttagc tggcatggtg ctatgggcgg tgggtgatat actgaattat ttctggggaa
    14221 gtaactcttt cactttcgtc atgttctctt gtgtcgcctt ttttggcacg gcctttgtaa
    14281 acagcctgaa ctgggcactg gtaccagata ccgtagatta cggtgaatgg aaaaccggta
    14341 ttcgtgccga aggttctgtt tataccggtt ataccttctt tcgtaaaatt tctgccgcac
    14401 ttgctggctt cttgccaggc attatgctga cgcaaattgg ttatgttccc aacatcgcgc
    14461 aaagcgatgc gacattacag ggtttgcgtc agctcatttt tatctggcct tgtgcactgg
    14521 caattattgc tgcattgacg atgggattct tttacacact caatgaaaaa cggtttgcat
    14581 tgattattga ggaaatcaac caacgcaaaa ataaagagat ggcgacagaa aaaaaaacgg
    14641 cttccgtaac gttataaatg tcaatagccg ctatttccat cctggtgggt ggcggcctcc
    14701 ctacgtttaa aaaatggact tattatgaaa aagattaatg caataatttt attatcttct
    14761 ctgacctctg cctcggtatt tgctggcgca tacgtcgaaa acagggaggc atataatctt
    14821 gcctccgatc agggcgaggt tatgctacgt gtgggttata acttcgatat gggcgcgggt
    14881 attatgttaa ccaataccta cacctttcag cgagaagatg aactaaaaca tggctataat
    14941 gaaattgaag gttggtatcc tttattcaaa ccaaccgata aattaaccat ccagcccggt
    15001 ggcttaatta atgataagag tatcagttcg ggtggtgcag tgtatctgga c
//
LOCUS       DAEEGA010000089        15030 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEEGA010000089 DAEEGA010000000
VERSION     DAEEGA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15030
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.089"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..236
                     /locus_tag="KLH46_003161"
     CDS             <1..236
                     /locus_tag="KLH46_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBE0379757.1"
                     /translation="LGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPA
                     LSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            246..884
                     /locus_tag="KLH46_003162"
     CDS             246..884
                     /locus_tag="KLH46_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBE0379758.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            887..2050
                     /locus_tag="KLH46_003163"
     CDS             887..2050
                     /locus_tag="KLH46_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBE0379759.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2134..3759
                     /locus_tag="KLH46_003164"
     CDS             2134..3759
                     /locus_tag="KLH46_003164"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBE0379760.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3876..4151)
                     /gene="yjfN"
                     /locus_tag="KLH46_003165"
     CDS             complement(3876..4151)
                     /gene="yjfN"
                     /locus_tag="KLH46_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBE0379761.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4300..4629)
                     /gene="bsmA"
                     /locus_tag="KLH46_003166"
     CDS             complement(4300..4629)
                     /gene="bsmA"
                     /locus_tag="KLH46_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBE0379762.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4811..5560
                     /gene="yjfP"
                     /locus_tag="KLH46_003167"
     CDS             4811..5560
                     /gene="yjfP"
                     /locus_tag="KLH46_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBE0379763.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5557..6312)
                     /gene="ulaR"
                     /locus_tag="KLH46_003168"
     CDS             complement(5557..6312)
                     /gene="ulaR"
                     /locus_tag="KLH46_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBE0379764.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6420..7484)
                     /gene="ulaG"
                     /locus_tag="KLH46_003169"
     CDS             complement(6420..7484)
                     /gene="ulaG"
                     /locus_tag="KLH46_003169"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBE0379765.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7839..9236
                     /gene="ulaA"
                     /locus_tag="KLH46_003170"
     CDS             7839..9236
                     /gene="ulaA"
                     /locus_tag="KLH46_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBE0379766.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9252..9557
                     /gene="ulaB"
                     /locus_tag="KLH46_003171"
     CDS             9252..9557
                     /gene="ulaB"
                     /locus_tag="KLH46_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBE0379767.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9567..10031
                     /gene="ulaC"
                     /locus_tag="KLH46_003172"
     CDS             9567..10031
                     /gene="ulaC"
                     /locus_tag="KLH46_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBE0379768.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10045..10695
                     /gene="ulaD"
                     /locus_tag="KLH46_003173"
     CDS             10045..10695
                     /gene="ulaD"
                     /locus_tag="KLH46_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBE0379769.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10705..11541
                     /gene="ulaE"
                     /locus_tag="KLH46_003174"
     CDS             10705..11541
                     /gene="ulaE"
                     /locus_tag="KLH46_003174"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBE0379770.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11541..12227
                     /gene="ulaF"
                     /locus_tag="KLH46_003175"
     CDS             11541..12227
                     /gene="ulaF"
                     /locus_tag="KLH46_003175"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBE0379771.1"
                     /translation="MQKLKQQVFEANMDLPRYVLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     EIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12356..12631)
                     /locus_tag="KLH46_003176"
     CDS             complement(12356..12631)
                     /locus_tag="KLH46_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE0379772.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12958..13353
                     /gene="rpsF"
                     /locus_tag="KLH46_003177"
     CDS             12958..13353
                     /gene="rpsF"
                     /locus_tag="KLH46_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBE0379773.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13360..13674
                     /gene="priB"
                     /locus_tag="KLH46_003178"
     CDS             13360..13674
                     /gene="priB"
                     /locus_tag="KLH46_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBE0379774.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13679..13906
                     /gene="rpsR"
                     /locus_tag="KLH46_003179"
     CDS             13679..13906
                     /gene="rpsR"
                     /locus_tag="KLH46_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBE0379775.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13948..14397
                     /gene="rplI"
                     /locus_tag="KLH46_003180"
     CDS             13948..14397
                     /gene="rplI"
                     /locus_tag="KLH46_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBE0379776.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14468..>15030)
                     /locus_tag="KLH46_003181"
     CDS             complement(14468..>15030)
                     /locus_tag="KLH46_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBE0379777.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     HTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3511 a         3666 c         4050 g         3803 t
ORIGIN      
        1 tgttaggtta cgttatccca ttatccagtg cggcaatcaa tgcggtgagt attccagact
       61 atttcgcctg gggcgggatt gcactggtga ttcagttact cgtttttgct ggtgtcagac
      121 tttatatgcc cgcattaagc gaaaaaatta ttaatcacaa taccgcagca ggaatgttta
      181 tgggaaccgc cgcgctggct ggcggtattt ttaacgcagc ttgtatgaca tggtaatgga
      241 cgatcatggc cagaaaacgc aaatcaagaa ataacagtaa aattggtcac ggagcgatca
      301 gtcgcattgg tagaccgaat aatccttttg aaccgcgtcg caatcgctac gcacaaaaat
      361 acttaacgtt ggcgctaatg ggcggtgccg cttttttcgt attgaaaggt tgtagcgata
      421 gtagcgatgt cgataacgac ggcgacggaa cattttacgc gacagtgcag gattgtattg
      481 atgacggtaa taatgccgac atttgcgcgc gtggctggaa caacgccaaa gcggcatttt
      541 atgccgatgt tccaaagaat atgactcagc agaactgtca gtctaagtac gaaaattgct
      601 actacgacaa tgttgaacag agttggatcc cggtcgtttc tggatttttg ttaagccggg
      661 ttattcgtaa agatcgcgat gagccgttcg tctataacag cggcggatcc tcatttgctt
      721 cccgccccgt ctggcgcaac acttccggtg attactcctg gcgcttcggt tctggcaaaa
      781 aagagtctta ctcttcaggc ggctttacca ccagaaaagc gtcgaccgtt tcgcgcggcg
      841 gctatggtcg ttcttccagc gcccgtgggc attggggagg ctaatcatgc tgagacacaa
      901 cgttcctgtg cggcgggatc tggaccagat cgccgctaac aacggtttcg actttcatat
      961 catcgacaat gaaatctatt gggacgagag tcgggcttac cgttttactc tgcgccagat
     1021 tgaagagcag atcgaaaaac cgactgcgga actgcatcag atgtgccttg aggtggtgga
     1081 tcgcgcggtt aaagacgaag agatcctgac gcaactggcg atcccgccgt tgtactggga
     1141 tgtgatcgcc gaaagctggc gcgcacgcga tccttcgctg tatggccgga tggattttgc
     1201 ctggtgtggc aatgcgccgg tgaagctgct ggagtacaac gccgatacgc cgacctcgtt
     1261 gtatgagtcg gcttatttcc agtggttgtg gctggaggat gcccggcgca gcggcattat
     1321 tccgcgtgat gccgatcagt acaacgccat tcaggaacgc ctgatttcgc gctttagtga
     1381 gctttacagt cgggaaccgt tttatttttg ctgctgtcag gacaccgatg aagacaggag
     1441 taccgtgctg tacttgcagg actgcgccca gcaggcaggg caggagtcgc ggtttatcta
     1501 cattgaagat ctcggtttgg gcgtcggcgg cgtactgacc gatcttgatg ataatgtcat
     1561 ccagcgtgca tttaagctgt atccgctgga gtggatgatg cgtgacgata acggtccgct
     1621 gctgcgcaag cgtcgcgagc aatgggtgga gccgttatgg aaaagtatct tgagtaataa
     1681 agggctaatg ccgctgcttt ggcgcttctt ccctggtcat cctaaccttc ttgcatcctg
     1741 gtttgagggt gaaaaatcgc agattgccgc tggcgaaagc tatgtgcgta aaccgatcta
     1801 ctcgcgcgaa ggcggaaacg tcaccatttt cgacggtcag aataacgtcg ttgaccacgc
     1861 tgatggtgat tacgccgatg aaccgatgat ctaccaggcg tttcaacctc tgccgcgatt
     1921 tggcgatagc tacacactca tcggtagctg gattgtcgat gatgaagcgt gcggaatggg
     1981 gatccgtgaa gataacacac tgatcaccaa agacacctca cgtttcgttc cgcattacat
     2041 tgctggataa gaatgtttta gcaatctctt tctgtcatga atccatggca gtgaccatac
     2101 taatggtgac tgccattgat ggagggagac acagtgcact ggcaaactca caccattttt
     2161 aatcaaccta taccattaaa taacagcaac ttatacctgt ctgatggcgc gctttgcgaa
     2221 gcggtaacgc gtgaaggtgc tggctgggat agcgattttc tagccagtat tggtcagcag
     2281 ttaggaacgg ctgaatccct tgaactgggg cggctggcga atgtaaaccc gcctgaactg
     2341 ttgcgctacg atgcccaggg gcggcgtctg gacgatgttc gtttccatcc cgcatggcac
     2401 ctgctgatgc aggcgttatg taccaatcgg gtgcacaatc ttgcctggga agaagacgct
     2461 cgctccggcg catttgtggc gcgcgcggcg cgttttatgt tacatgcgca ggttgaggct
     2521 gggtcgttat gtccaataac gatgaccttt gccgccacgc cattgttgtt acagatgtta
     2581 cccacgccgt ttcaggactg gaccacgccg ctgctgagcg atcgctacga ttctcactta
     2641 ttgccaggtg ggcaaaaacg cggtttgttg attggcatgg gaatgacgga aaagcagggc
     2701 ggttccgatg tcatgagcaa caccacccgt gcagagcgtt tggaagatgg ctcttatcgg
     2761 ctggtggggc ataaatggtt tttctcggtg ccgcaaagcg atgcgcatct ggtgctggcg
     2821 cagaccgcgg gcggtctgtc ctgctttttt gtgccgcgct ttttgcctga cgggcaacgc
     2881 aacgcgattc gcctcgagcg gctgaaagat aagctgggta atcgctctaa cgccagttgc
     2941 gaagtggagt ttcaggatgc cattggctgg ttattggggc aggaagggga aggaattcgt
     3001 ctgatcctga aaatgggggg gatgacgcgt tttgattgcg ccctgggtag ccatgccatg
     3061 atgcgccgtg cattttcgct ggcgatttat catgcacatc aacgccatgt ttttggtaat
     3121 ccattgatcc aacagcccct tatgcgtcat gtcttaagtc gcatggcact tcagcttgaa
     3181 gggcaaacgg cgttgctgtt tcgtcttgcg cgagcgtggg accggcgtgc cgatgccaaa
     3241 gaagccctgt gggcgcgttt atttacgcct gcggcaaaat ttgtgatctg caaacgaggt
     3301 atgccgtttg tggccgaagc gatggaggtg ctgggcggca ttggttattg cgaggagagc
     3361 gagctgccgc ggctttaccg ggagatgccg gtaaacagta tttgggaagg ttccggcaat
     3421 attatgtgcc tggatgtgtt gcgcgttctc aataagcaag cgggcgtata cgacttattg
     3481 tcggaagcat ttgtggaagt gaaagggcag gatcgctatt ttgatcgcgc ggttcgtcgt
     3541 ttacagcagc agctgcgtaa gccagctgaa gaactggggc gagagattac tcatcagcta
     3601 ttcctgctgg gctgcggtgc gcaaatgttg aaatatgctt ctccgccaat ggcgcaggcg
     3661 tggtgtcagg tgatgttaga tacgcgcggc ggcgtacggt tgtcagagca gatccagaat
     3721 gatttattgc tgcgggcgac ggggggagtg tgtgtgtaag cgtatacgac tgatgcgacg
     3781 ctggtttcga ttaactaaat gaaatatgtg aaaattgtag gccggacaag gcgctcgcgc
     3841 cgcatccggc attgttcatt tgctgtagat gacactcatg catacagtat cgcctgtacg
     3901 cgccagttat caggctgctg gtcttcgtac atggtaataa tgcggtacca tgacgcacct
     3961 tgttcatcgg cctttaacgc cacgatacgt tccacgtcct gcggatcccc tgaaatattg
     4021 ctaactgaga tctgacctat ttcattcagg cccgtcacgc agtcagcact ggcgaattct
     4081 gcagactgtg ccgtggcgct cgcaggataa gtcactaatt gcagcgaggg tgaggcaagt
     4141 aattgtttca tcgtcagctc catttttcgt ttccccacat gttgcagggc attccatcgc
     4201 ggtaataaac gcccaagctt ccagccggta atttattgtg cacaggtaaa cgcataggga
     4261 cgcaaagcaa tgtaaagctg actatgtaag ttgtctgatt tatttacggt acaaaatggc
     4321 ttgtgaatac cactgtcctg tcacgatggt ttcgtccacc atgacgacaa cgtaataatc
     4381 agcttttgca gcgacagctt ttgctctgat ttctgctaat gcgtcatccg gagaaccccg
     4441 aaccatcgtg cttacgctac ctattcgctg taacccttgc gtctggtcgc ggcgaatctc
     4501 ttgcggatgg tccgttactg gcggtgctgg ctgtggcgta ccttgcagtg cgctacaggc
     4561 acttaacatc aacaccaata ataaactggc aaaccggtaa ataacgctat tacgtttcct
     4621 gctaaccata gtgtagtgcc ttattaattt aactttgggg gaatgttccc gaattgttac
     4681 ctggtacgcg attttcgaat gaaaacgtga cgaaagcgta acccacatct caacattatg
     4741 aactaatacc gccagcagaa acttgtgcta aggatttctt catgtcatac cagacaaaaa
     4801 ggagagacag atgattgaaa tagaatcacg cgagctggca gatattcccg ttcttcatgc
     4861 ttatcctgtc gggcaaaaag ataccccgtt accgtgcgta attttttatc acggctttac
     4921 ttcatccagc ctggtgtata gctattttgc cgttgcgctg gcgcaggctg gtttgcgggt
     4981 gatcatgccg gatgcgcccg atcacggtag ccgttttagt ggcgacgaag cgcggcggtt
     5041 aaatcaattc tggcaaatct tgctacaaag tatgcaggaa ttcactactt tacgtgcggc
     5101 aatagccgaa gaaaactggc tgcttgatga ccgtctggca gtcggtggcg cgtcgatggg
     5161 cgcgatgacg gcactgggaa ttaccgctcg ccaccccacg gtgaaatgta ccgccagcat
     5221 gatgggatcg ggctatttta catcgctcgc ccgttcactg tttccaccgc tgatacctga
     5281 aacggcagca cagcagaatg aattcaataa aattgtcgcg ccgctggcag agtgggaagc
     5341 gacaaaccac ctggaacaac ttggggacag acctctactg ctgtggcatg gcctcgacga
     5401 tgacgttgtg cctgccgacg aatcactacg tttgcagcag gccttaagcg agacgggacg
     5461 ggataaactg ctaacctgtt catggcagcc aggcgtgcgt caccgtatta cgcctgaggc
     5521 gttagatgct gccgtgacat ttttccgcca gcatctttaa acacgcagaa tgctgacccc
     5581 ttgcgcttcc agttgttgca ggatttccgg gttagcattt ttgccggtga tcagcatatc
     5641 gatttgatcg gcacggctaa aaagcattcc cgcgcgttcg ccaatcttac tgctatcaac
     5701 cagtaccacc agtttcccta ccacgctcag catcttctgc tctgccattg ctgtcagcat
     5761 atcggtttta tacaaccctt ctgcggtcag cccttttccg ctggtaaaca tccagtgccc
     5821 ggcatagaga ctgttttcgc tgccctgcgg gcttaaagtg atggactgac ttttgttgta
     5881 ctgtccaccc ataatgatca cgctgtcatg ttcttgatcg atcaggtaat ttgccagcgg
     5941 tagataatta gtgatgattt gcactggctt gccacacatt tcccgcccaa gcagaaacgc
     6001 ggtggagccg cagttgatga ctacgctttc gcccggatta accagctgcg aggccgcttt
     6061 agcaatacgt actttttcat cgtgattctg cgcctgatgc agattcatcg gcgtccagcg
     6121 cgggcgctgt tgggtaatag cttctgcgcc attgcgcacc tttttcagtt tgccgctttc
     6181 gccaagttta ttgatatcgc gtcgcgcagt ggcaggcgaa attcccagac gctcaacgac
     6241 tttctcaacg gtcacaaagc ccaattgtgc gagcatttcc aggaggattt gatgtctttg
     6301 tgcttcagtc atgagctatt ccgataaaat ttgatttttt tagatgatat ttgaaatagc
     6361 caggaaatac tacgccggat agcgcgaact tctccacgct atccggtata aacgacagat
     6421 tacaggaatg acttgaacgg cagatccggt tcaatagtga agcaatcatc gaaaccgcgc
     6481 ggatagtggt actcgaagtt gtctttatcc agcggccagg taaatttgcc gccaacctgc
     6541 cagataaacg gcttgaagcc atacttcaga cgatcttttt tgatctccca cagcacgcgg
     6601 atctcttgcg gatcggcctg gaagtttgac cagatgtcgt ggtggaacgg gataaccact
     6661 ttcgcattca gggcttcacc catacgcagc atatcggcgc tggtcatttt gtcggtaata
     6721 ccgcgcgggt tctcaccata cgatcctaac gccacatcga tctgatgttc gttaccgtgc
     6781 ttcgcataat agttagagta gtgggaatcg ccactgtgat atagggaacc gccaggggtt
     6841 ttgaacaggt agtttactgc gcgatcgtcc atgccatctg gcagtacgcc agccgctttt
     6901 tgatcggcag gcagggtgat cagtgcagta cggtcgaaag cgtcaagcgc atgaatttca
     6961 atgtctttca cttttacaac atcgcccggt ttgaccacga tgcaacgctc tttcggtacg
     7021 ccccagccaa tccacaaatc aacgcaggtt ttcggtccaa tgaatggtac gtcatcagca
     7081 cagttctgca tcacggcagc agcgacgtta acgtcgatat gatcgttgtg atcgtgagtc
     7141 gccagtaccg cgtcgatctg gcgaatcgca aacggatcaa gaacaaacgg ggtggtgcgt
     7201 aggtttggct gcagtttttt cacgccagcc atgcgctgca tctggtgacc ctgtttcatt
     7261 aacgggttac cgtgactttg tttgccagtg ccgcaccaga aatcaacgca aacgttggtg
     7321 ccaccttctg atttcaacca gatcccggtg cagcctagcc accacatcgc aaatgtgcca
     7381 ggggcgacct gctcttgttc aatttcttca ttcagccagc taccccactc cgggaaagtg
     7441 ctcaggatcc aggattctcg ggtgatactt ttcactttac tcatcgccat ttaccttcat
     7501 gatagttcaa ttcgaatcaa catgtgattg gttttgatta atcctgacac tattccttca
     7561 ggaaggcaat gaccattttt tgactttttg ccagggaagt tgttgttgat tttgagtgtg
     7621 gaaagattta atggaatgtg tagttaaatt aactaaatga atttaaataa ataattgttt
     7681 cgttgtgtga atcacactct atccgtgtgg aattatttgc gggtcgcgcc acatttaatc
     7741 ataaataatc ttgttgtgat tacttttgaa aattagagtg agtacacaac attccgggtg
     7801 tgcggaatac ccggttacct cttcttcagg agatcgttat ggagatcctc tacaacatct
     7861 ttaccgtgtt ttttaaccag gtcatgacca atgccccgtt gttgctgggt attgtgacct
     7921 gtctgggcta catcctactg cgcaaaagtg tcagcgttat tattaaaggc acgattaaaa
     7981 ccataattgg tttcatgttg ttgcaggcag ggtccggcat cctcaccagc accttcaaac
     8041 cggtggtggc gaaaatgtcc gaagtctacg gcattaacgg cgcaatttcc gatacttacg
     8101 cgtcgatgat ggcaaccatc gaccgcatgg gcgatgccta tagctgggta ggttacgcgg
     8161 tgttgttagc gctggcgctg aacatctgtt acgtgctgct gcgtcgcatt accggtattc
     8221 gtaccatcat gctgactggt catatcatgt tccagcaggc ggggctgatt gccgttacgt
     8281 tgtttatttt cggctactcc atgtggacaa ccattatctg cacggcgatt ctggtttcgc
     8341 tctactgggg cattacctcc aacatgatgt acaagccgac tcaggaagtg acggacggct
     8401 gcggtttctc catcggtcac cagcaacagt ttgcatcatt aattgcctat aaagttgcgc
     8461 cgttcctcgg caaaaaagag gaaagcgttg aagacctcaa actgccaggc tggctgaata
     8521 ttttccacga caacatcgtc tccacggcga ttgtcatgac catcttcttt ggtgccattc
     8581 tgctctcctt cggtatcgac accgtgcagg cgatggcagg caaagtgcac tggacggtct
     8641 atatcctgca aaccggtttt tccttcgcgg tggcgatctt catcatcact cagggtgtgc
     8701 gcatgtttgt ggcggaactc tctgaagcgt ttaacggtat ctcccagcgc ctgatccctg
     8761 gtgcggttct ggcgattgac tgtgcggcta tttatagctt cgctccgaac gccgtggtct
     8821 ggggctttat gtggggcacc atcggtcagc tgattgcggt tggcatcctg gtcgcctgcg
     8881 gttcctcgat cctgattatc ccaggcttta tcccgatgtt cttctccaac gccaccatcg
     8941 gcgtgttcgc taaccacttc ggcggctggc gtgcggcgct gaagatttgt ctggtgatgg
     9001 ggatgatcga aatctttggt tgcgtctggg tggtgaaact taccggtatg agtgcctgga
     9061 tggggatggc ggactggtcg attctggcac cgccgatgat gcagggcttc ttctccatcg
     9121 gtatcgcctt tatggccgtc atcattgtaa ttgcactggc ttatatgttc ttcgctggcc
     9181 gcgcactgcg cgcagaagaa gatgcagaaa aacaattggc agaacagtct gcttaataag
     9241 gagttttgat tatgaccgta cgtattctgg ctgtgtgtgg caacggacaa ggcagttcca
     9301 tgatcatgaa gatgaaagtg gaccagtttt taacccaatc aaacattgac cacacggtaa
     9361 atagctgcgc ggttggcgaa tacaaaagcg agttgagcgg cgcggatatc atcatcgctt
     9421 ctacgcacat tgcgggcgaa atcaccgtga ccggcaacaa atacgtggtt ggcgtgcgca
     9481 acatgctctc tcctgccgac tttggcccga aactgctgga agtgatcaaa gcgcatttcc
     9541 cgcaggatgt gaagtaagga cgcgccatga aattacatga ttcgctggcg gagaataaat
     9601 ccatccgcct gcaggctgaa gctgagacat ggcaggacgc agtaaaaatc ggcgttgacc
     9661 tgctggtggc ggcggatgtt gtcgagccgc gttactacca ggcgattctg gatgcggttg
     9721 aacagcacgg tccgtacttt gtgctcgcac caggcctggc aatgccgcac ggtcgcccgg
     9781 aagagggcgt taagaaaacc ggttttgcgc tggtgacgct gaaaaagccg ctggagttca
     9841 accacgaaga taacgacccg gtggatatcc tcatcaccat ggcggcggtc gatgccaaca
     9901 cgcaccagga agtgggcatc atgcagatcg tcaacctgtt tgaagatgaa gaaaatttcg
     9961 accgtttacg tgcctgccgt accgagcagg aagtactgga tctcattgac cgcaccaacg
    10021 cggcagctta agaaggaatt gaagatgtca ttaccgatgt tgcaagtcgc gctggacaac
    10081 cagactatgg atagcgccta cgaaaccact cgcctgattg ccgaagaagt cgacattatc
    10141 gaagtgggta ccattctgtg cgtgggcgaa ggcgtgcgtg cggttcgcga tctgaaagcg
    10201 ctctacccgc acaaaatcgt gctggcagac gccaaaattg ccgatgcggg caaaattctt
    10261 tctcgtatgt gcttcgaagc caacgctgac tgggtgaccg tgatttgttg tgcggatatc
    10321 aacaccgcca aaggtgcgct ggacgtagca aaagagttta acggcgacgt gcagatcgaa
    10381 ctgaccggtt actggacctg ggaacaggcg caacagtggc gcgacgcagg tattcagcag
    10441 gtggtttatc accgcagccg tgacgcgcag gccgctggcg tggcgtgggg tgaagccgat
    10501 atcactgcga tcaaacgtct ttccgatatg ggcttcaaag tcaccgtcac cggtggcctg
    10561 gcgctggaag atctgccgct gttcaaaggc attccaattc atgtgtttat cgccggtcgc
    10621 agcatccgtg atgccgcttc tccggttgaa gccgcgcgtc agttcaaacg ttccatcgcc
    10681 gaactgtggg gctaaggagc ggatatgttg tccaaacaaa tcccgcttgg catctatgaa
    10741 aaagcgctcc ccgccgggga gtgctggctg gaacgcctgc gactggcaaa aacgttgggc
    10801 ttcgattttg tcgagatgtc ggtagatgaa actaacgagc gcctgtcgcg cctcgactgg
    10861 agccgcgaac agcgtctggc gctggtgaat gctattgttg aaaccggcgt gcgtgtgccg
    10921 tccatgtgcc tttctgccca tcgtcgtttc ccgttgggca gcgaagatga cgcggtgcgg
    10981 gcgcaggggc tggagattat gcgtaaagcc atccagttcg cccaggacgt tggcattcgc
    11041 gtgatccagc tggcgggcta tgacgtttac tatcaggaag ccaataacga aacgcgtcgt
    11101 cgtttccgtg acggcctgaa agagagcgtt gagatggcga gccgcgcgca ggtgacgctg
    11161 gcgatggaga tcatggatta tccgttgatg aactccatca gcaaggcgct gggttacgcg
    11221 cactatctca acaacccgtg gttccagctc tacccggata tcggcaacct gtcggcgtgg
    11281 gacaacgatg tgcagatgga gttgcaggcc ggaatcgggc atatcgtcgc ggtgcatgtg
    11341 aaagacacca aaccgggcgt cttcaaaaac gtgccatttg gcgaaggtgt ggtggatttc
    11401 gaacgttgct tcgaaacgct caaacagagt ggctattgcg ggccgtacct gattgagatg
    11461 tggagcgaaa cggcggaaga cccggcggca gaagtggtga aagcgcgcat ggccaaagca
    11521 ggcatggtgg aggcggcata atgcaaaagc taaaacagca ggtgtttgaa gccaacatgg
    11581 atctgccgcg ctatgtgctg gtgaccttta cttggggcaa cgtcagcgct atcgaccgcg
    11641 aacgcgggct ggtggtgatc aagcccagcg gcgttgccta cgaaaccatg aaagcagacg
    11701 atatggtggt ggtcgatatg agcggcaagg tggtggaagg ggagtatcgc ccatcttccg
    11761 acactgcgac gcatctcgaa ctctaccgtc gttacccgtc gcttggcggc attgtccata
    11821 cccactccac tcatgccacc gcatgggcgc aggcggggct ggcgatcccg gcattaggca
    11881 ccacgcacgc tgactacttc tttggtgata tcccgtgtac gcgtggatta agcgaagaag
    11941 aggtgcaggg tgagtatgaa ctgaacactg gcaaagtgat tatcgaaacg ctgggcaacg
    12001 ccgagccgct gcatacgccg gaaattgtgg tgtatcagca cggaccgttc gcctggggga
    12061 aagatgccca cgacgcggtg cataacgcgg tagtgatgga agaagtggcg aaaatggcgt
    12121 ggattgcgcg cagtattaat ccacaactca atcatatcga cagctacctg atgaataaac
    12181 acttcatgcg taagcacggt cctaacgctt attacgggca gaagtaaaac acgcgctgcg
    12241 gaaatttcct tcctcgggag ataactggtc taattccgca gccgtttttc aaaaaaagcc
    12301 ccctgcgaag ggggcaaagc aaactatggc aatgtttcgt tggttatacc tggtgctagc
    12361 gataaatatc cgcgctggcg tgcatattgc cgttactccc cggttcccgc atcagaatta
    12421 cgtggtagta cgttgcgcct tgcgcatctg tttcttcatt taatgcctga cgtgcttcgc
    12481 tttcagtggc gaaattatga ttgatataaa tcacgcctaa gctttgcaca tcgtccatat
    12541 ttctggcctg gtggttatta atttcaatgg ctgcccatgt atttgcactt agcaaaagca
    12601 cagccagaag ggctaaaact cgactgaaca tagatacctc ctcgacggct gactttgtgt
    12661 gctctcctct gtgatgatct tctgatttaa ttttaatcaa tgataaagaa gttgatggtg
    12721 accatttctg atgcagttgt tcaaaaaaac actgtgacgg aatgtgatga acttcaaatc
    12781 agcggtttag aggttatttg cgaaagggga gatttatttc tgctccgccc ttgagtttag
    12841 cggggtatgc cagtactata acgcgtcatt tttcagccga cctttaacac gttccttgcc
    12901 tccccgggat tcggctgacc cagacaggag gctgaataat ccgtaaggag caattcgatg
    12961 cgtcattacg aaatcgtttt tatggtccat cctgaccaga gcgaacaggt tccgggcatg
    13021 atcgagcgct acactgctgc catcactggt gcagaaggca agatccaccg tctggaagac
    13081 tggggccgcc gtcagctggc ttacccgatc aacaaactgc acaaagcaca ctacgttctg
    13141 atgaacgttg aagctccgca ggaagtgatc gatgagctgg aaactacctt ccgcttcaac
    13201 gatgccgtta tccgcagcat ggttatgcgt accaagcacg ctgttaccga agcatctccg
    13261 atggttaaag cgaaagacga gcgccgtgag cgtcgcgatg atttcgcaaa cgaaaccgct
    13321 gatgatgctg aagctgggga ttctgaagag taatttctga tgaccaaccg tctggtgttg
    13381 tccggcaccg tgtgcaggac tccccttcga aaggtcagtc catcaggaat tcctcactgc
    13441 cagttcgtgc ttgagcatcg ttctgtgcag gaggaagccg gctttcaccg gcaggcatgg
    13501 tgtcaaatgc ccgttattgt tagcggacac gaaaaccagg ccattactca cagtataacg
    13561 gtcggcagtc gcataaccgt tcaggggttc atttcatgcc acaaggcaaa gaacggactg
    13621 agcaaaatgg ttttgcatgc cgagcagatt gaattgatag attctggaga ctagccatat
    13681 ggcacgttat ttccgtcgtc gcaagttctg ccgtttcacc gcggaaggcg ttcaagagat
    13741 cgactataaa gatatcgcta cgctgaaaaa ctacatcacc gaaagcggta agattgtccc
    13801 aagccgtatc accggtaccc gtgcaaaata ccagcgtcag ctggctcgcg ctatcaaacg
    13861 cgctcgctac ctgtccctgc tgccgtacac tgatcgccat cagtaatcgg tcacggtcca
    13921 ttaatacgac tttgagagga taaggtaatg caagttattc tgcttgataa agtagcaaac
    13981 ctgggtagcc tgggtgatca ggtaaacgtt aaagcgggct atgctcgtaa cttcctggta
    14041 ccgcagggta aagctgttcc agctaccaag aaaaacattg aattcttcga agcacgtcgc
    14101 gctgaactgg aagctaaact ggctgaagtt ctggcagctg ctaatgctcg cgctgagaaa
    14161 atcaatgcac tggaaactgt taccatcgcg tctaaagctg gcgacgaagg taaactgttc
    14221 ggttccatcg gtactcgcga catcgctgac gctgtaactg cagctggcgt tgaagtggct
    14281 aagagcgaag ttcgtctgcc gaacggcgtt ctgcgtacca ctggcgaaca cgaagtgagc
    14341 ttccaggttc acagcgaagt attcgcgaaa gtgatcgtaa acgtagtagc tgaataattc
    14401 gttattcaac gagacgtaaa aacgccgacc attggtcggc gttttgcttt ctatttttcg
    14461 tcaggcatta atttcgcaag tagatccatt caccattctc ttgtgtccat aagtcaggat
    14521 ggtttgctgg cgttagatgc atacgatcac tccttatgtc tcggtcctgc ggcactcgct
    14581 cgaaacctaa acccgagtag attgcggctg aatttgataa ggaacgtaga ccaattttca
    14641 aatcatgggg taaatcagaa tacgcgttta gcaatgcagc aatgatgaat ccgagggcat
    14701 tagaaacgcc tgtaacagtg tgaagttggt cgatataaag atcgattgtt ggtagttcag
    14761 atgaccagca attaggcatt tgggcaggaa gggttacaac gtatcctatc gtttggccgg
    14821 tatagtctgg agtactataa ccaggtccca aaacaacctc gtgtattgtg tatttactgt
    14881 tgggttcgaa ggattcgaca tcttgcaata aatatttcag ttctgcggtt aacctcgcac
    14941 ccgcgtaagc tttattgtat ttctgggcgt gaatattatc taatgtagct tcatggttcc
    15001 atccaaaaga attatcacca ttcattgtta
//
LOCUS       DAEEGA010000090        14920 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEEGA010000090 DAEEGA010000000
VERSION     DAEEGA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14920
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.090"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="KLH46_003182"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="KLH46_003182"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBE0379778.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="KLH46_003183"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="KLH46_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBE0379779.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="KLH46_003184"
     CDS             complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="KLH46_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBE0379780.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2977..3060
                     /locus_tag="KLH46_003185"
     CDS             2977..3060
                     /locus_tag="KLH46_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBE0379781.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="KLH46_003186"
     CDS             complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="KLH46_003186"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBE0379782.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="KLH46_003187"
     CDS             complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="KLH46_003187"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBE0379783.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5583..7625
                     /gene="prlC"
                     /locus_tag="KLH46_003188"
     CDS             5583..7625
                     /gene="prlC"
                     /locus_tag="KLH46_003188"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBE0379784.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7633..8385
                     /gene="rsmJ"
                     /locus_tag="KLH46_003189"
     CDS             7633..8385
                     /gene="rsmJ"
                     /locus_tag="KLH46_003189"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBE0379785.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="KLH46_003190"
                     /pseudo
     CDS             complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="KLH46_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="KLH46_003191"
     CDS             complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="KLH46_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBE0379786.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11049..11384
                     /gene="uspB"
                     /locus_tag="KLH46_003192"
     CDS             11049..11384
                     /gene="uspB"
                     /locus_tag="KLH46_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBE0379787.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="KLH46_003193"
     CDS             complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="KLH46_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBE0379788.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13186..14385
                     /locus_tag="KLH46_003194"
     CDS             13186..14385
                     /locus_tag="KLH46_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBE0379789.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3713 a         3788 c         3928 g         3491 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaagac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt
     2221 aacagaaatg acatattgcg ctcctgattg ttgcaggtag tgtctctctt cgaagcggat
     2281 aagtcaaaaa catatatgac ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga
     2341 attttgagtt ggctattcat ttgaaaggcg gtttgaatca ggacgtaaaa aaaccgactt
     2401 tgcgtcggtt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     2461 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     2521 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     2581 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     2641 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     2701 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     2761 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     2821 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     2881 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     2941 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     3001 cttggggcgt taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga
     3061 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     3121 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     3181 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     3241 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     3301 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     3361 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     3421 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     3481 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     3541 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     3601 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     3661 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     3721 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     3781 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     3841 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     3901 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     3961 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     4021 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     4081 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     4141 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     4201 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     4261 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     4321 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     4381 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     4441 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     4501 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     4561 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     4621 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     4681 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     4741 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     4801 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     4861 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     4921 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     4981 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     5041 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     5101 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     5161 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     5221 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     5281 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     5341 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     5401 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     5461 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     5521 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     5581 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     5641 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     5701 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     5761 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     5821 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     5881 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     5941 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     6001 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     6061 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     6121 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     6181 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     6241 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     6301 gcacccgcgc ctccgatcaa ggcccgaatg ccggtaagtg ggataacagc aaggtgatgg
     6361 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     6421 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     6481 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     6541 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     6601 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     6661 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     6721 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     6781 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     6841 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     6901 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     6961 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     7021 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     7081 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     7141 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     7201 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     7261 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     7321 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     7381 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     7441 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     7501 agtcgttcct cgacaacatt ctgagccgtg gtggttcaga agagccgatg gatctgttca
     7561 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     7621 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     7681 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     7741 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     7801 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     7861 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     7921 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     7981 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     8041 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     8101 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     8161 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     8221 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     8281 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     8341 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     8401 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     8461 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     8521 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     8581 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     8641 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     8701 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     8761 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     8821 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     8881 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     8941 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     9001 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     9061 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     9121 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     9181 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     9241 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     9301 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     9361 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     9421 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     9481 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     9541 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     9601 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     9661 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     9721 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     9781 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     9841 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     9901 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     9961 gaaaaagttt acatctgtca gagattctta atattgatgc ggtaaataga ttaatacact
    10021 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
    10081 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
    10141 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
    10201 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
    10261 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
    10321 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
    10381 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
    10441 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
    10501 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
    10561 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
    10621 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
    10681 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
    10741 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
    10801 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
    10861 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
    10921 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
    10981 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
    11041 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
    11101 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
    11161 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
    11221 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
    11281 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
    11341 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
    11401 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
    11461 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
    11521 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
    11581 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
    11641 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
    11701 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
    11761 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
    11821 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
    11881 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
    11941 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
    12001 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
    12061 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
    12121 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
    12181 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    12241 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    12301 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    12361 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    12421 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    12481 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    12541 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    12601 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    12661 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    12721 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    12781 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    12841 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    12901 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    12961 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    13021 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    13081 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    13141 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    13201 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    13261 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    13321 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    13381 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    13441 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    13501 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    13561 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    13621 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    13681 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    13741 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    13801 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    13861 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    13921 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    13981 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    14041 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    14101 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    14161 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    14221 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    14281 ggcgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    14341 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    14401 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    14461 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    14521 gtgtatttat tctcagagaa tatatattac tgaaataaaa aacacaaaga ataaccaaag
    14581 gaaattattt gaaacgtaaa aggtatatat ttttaatctg taaaatttaa aatcacctcc
    14641 tctggattga atcaacttgc tctatttaag acgattgttt ttacattttc accacctgta
    14701 tcaggtggtg tttttttatt ggcggcaatg cctttatctt atttttatta cgttgtaaat
    14761 atggctattc ctaaatgaaa tttgatttgc tcagtttatt tgtgatgtca ggggagtgct
    14821 tgccatgaga gtcgtgatat tattattcat catttttagg taatgctgcc aacttactga
    14881 tttagtgtat gatggtgatt ttaaggtgct tgcgtggctt
//
LOCUS       DAEEGA010000091        14565 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEEGA010000091 DAEEGA010000000
VERSION     DAEEGA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14565
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.091"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..581
                     /locus_tag="KLH46_003195"
     CDS             <1..581
                     /locus_tag="KLH46_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBE0379790.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDYFVVI"
     gene            complement(635..1357)
                     /locus_tag="KLH46_003196"
     CDS             complement(635..1357)
                     /locus_tag="KLH46_003196"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBE0379791.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(1359..2530)
                     /locus_tag="KLH46_003197"
                     /pseudo
     CDS             complement(1359..2530)
                     /locus_tag="KLH46_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="KLH46_003198"
     CDS             complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="KLH46_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBE0379792.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="KLH46_003199"
     CDS             complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="KLH46_003199"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HBE0379793.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="KLH46_003200"
     CDS             complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="KLH46_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBE0379794.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="KLH46_003201"
     CDS             complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="KLH46_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBE0379795.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8790..9515)
                     /gene="phoU"
                     /locus_tag="KLH46_003202"
     CDS             complement(8790..9515)
                     /gene="phoU"
                     /locus_tag="KLH46_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBE0379796.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(9530..10303)
                     /gene="pstB"
                     /locus_tag="KLH46_003203"
     CDS             complement(9530..10303)
                     /gene="pstB"
                     /locus_tag="KLH46_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBE0379797.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSCADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(10395..11285)
                     /gene="pstA"
                     /locus_tag="KLH46_003204"
     CDS             complement(10395..11285)
                     /gene="pstA"
                     /locus_tag="KLH46_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBE0379798.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLVEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(11285..12244)
                     /gene="pstC"
                     /locus_tag="KLH46_003205"
     CDS             complement(11285..12244)
                     /gene="pstC"
                     /locus_tag="KLH46_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBE0379799.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(12331..13371)
                     /gene="pstS"
                     /locus_tag="KLH46_003206"
     CDS             complement(12331..13371)
                     /gene="pstS"
                     /locus_tag="KLH46_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBE0379800.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13619..>14565)
                     /locus_tag="KLH46_003207"
     CDS             complement(13619..>14565)
                     /locus_tag="KLH46_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0379801.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         3757 a         3718 c         3316 g         3774 t
ORIGIN      
        1 tcggggcgct tacattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg
       61 ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct
      121 tttattgcgt tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg
      181 cagaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt
      241 atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa atgctgaaag
      301 agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt
      361 gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg
      421 atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag
      481 ctaaatgcct gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg
      541 gcaagctgag actgtggggt gattatttcg tagttattta aataatgaga acaggccgga
      601 gcgtaattca tacatccggc cttatttctt cagcttaacc gaacgctaat tctgcggcag
      661 cggctttatc agcgattacc atcagcgaag gatgcagctg caacacggaa gctggaacat
      721 cttcggtaac cggaccttgc agcacatttt tcagtgcctg cgctttacct gcgccactga
      781 caattatcag caagtttttc gccgccatga tgcttttcgg ccccatcgtc acgtagctgt
      841 caggcactaa cgaaaagtcg ccgcccaact caccatgcgc cacaatatca accatctctc
      901 cctggatcgg gaactccacg gtttgctcat ggaaatgggt cgtattcggc aaattgccac
      961 aaaaatgacc atctgcacct aatcccagca ccaccaaatc caggccgcct tcacgcgcca
     1021 gtttctgatc atgctcgcgg tagttatcaa tagtgagctt ctggatattc tcttctttga
     1081 tccccgcagg ggtgaaaaac agattacgca gattggtaat cgttacgcct tctccctctt
     1141 tgccgcgaaa gggaatttca tcgaaattat agaaatagca gttatcgtac caaggcttac
     1201 ctttaaccag ggtggtgagg tattcataca tgcctttggg cgtgctaccg gcggtaattg
     1261 ccaggttaac acgacgcgtc ttcgacatat aaccaagcag atggtgtgcc gcgacacggc
     1321 tcatttcctg gtaatcttcg gtaatgatta atttcatctt aatgccttat tttaaatttc
     1381 tgtacgaagg ctgcataggc aggccgccag acatccattt catggttcag gccgggatat
     1441 tcctggtaat caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc
     1501 ttgccggtta cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta
     1561 atagctgccg gatcgttcag ccgggccgcg acaccttcat ccggtacggt tgtcgtggta
     1621 acaccactga atgtggccag ccaaagcttt ccagatgatt cattccggaa accagcgcct
     1681 ggtacccgcc ttgtgaaagc cctgccagcg cgcgaccatc ggcatcttta cggacactaa
     1741 aacgcttgct aatcagcggg ataatatcgt tcatcagttc gcgatctgcc gctttagcat
     1801 tcagcggata aaagacttta cgtctttcct gaggcacgaa atcttcggga ataatgccct
     1861 tcgcatcggt ttcggtatcc gggatcacca ccagcatcgg tttaattttc ccttcagcca
     1921 gcaggttatc catgatttgc gggatacgcc cctgatcgat agcggaacgt ccggtatcac
     1981 caaaaccgtg atagaaatag agcactggca aaggctcgcc catgccggtg tatcctggcg
     2041 gggtccagac atacatctga cgttcagatt gcaatgcgtt ggagtggtaa gttatggcga
     2101 tcaaatcacc atgcgcaaca gaacgcgtat ccagataact gcctggcacc agaatcatac
     2161 tggagttaac ctggcgctga ggcttagtca ttgcggtgcc tgtatcaatg ctgcgtacac
     2221 catcaacatt gaaaaaatac tcgtacaggt tgcctttcag gataggtgtg cgccacgacc
     2281 agactcctgc ttcgtctttg gtcatcgggt gaatattgtc cggaaccgga acacccacca
     2341 ctacagagac attttttgcc ccaggggcaa agtagcgaaa ggtcacgctg ttatcggcat
     2401 tgacctgagt cacatactgt ttcaccggga tagtgggtgc cgggctggct ggcatatccg
     2461 ctgctatggc ccactgagca gaaataccgc tggcaatagc cagcgttaaa gcagcaattt
     2521 ttatattcat cgtaatattc ctttcattaa gttccattac caccagattt cagcctgggc
     2581 accgacagca aactgggcgt ctttattgtc ttcgaaggtg aagccatcca gttcgttttc
     2641 cagggctttg atatacgtgg cgtagaaacg aatttccaga cgcgaggtca acatactggt
     2701 attgacttta aaggtatgga agagcgtggt tttataacca gactcattaa atttattact
     2761 attcgcatct ttgttttgct gggtgaaata gcccagttca acacctgtct ggttatattg
     2821 gtcccaaata taggccgggc gaacaaccgc acgaatagat tcgaaatcag agtgtgcgcc
     2881 tgtttcgtag ctataaatat tgttaccgaa ggagtaaaca atcgcgttag ccacaataaa
     2941 atgatcgccg atataggctt cgccctgcga ggtcagacga actgctgttc cgccggtgtg
     3001 atcaccataa taacgaccat taaaggtggt aaatggactt gcgccagcat aacggccaaa
     3061 gttcctggcg atagagttat tcgcaaccag gaaggagaat tcgttgaagc cacctttatc
     3121 aaatttctgc gttaaagatg tgccgaacat ccaggtatct ttccagtcat aatacccgtt
     3181 attatcctga ttatcttttt ccgatgcgct ttcgtttgcc gtgacataac gaccactcac
     3241 cattaaggtc gctttatccc ataacgggat atctttatag cgtaaatcaa tagtatgggt
     3301 attaatctgc tgtttgtttt gcaggctgcg atcgtaatca tcgacatctt cgcgaaccag
     3361 cgcgatatca attttacccg gaccgacttt ccagttttcc agacctacac ccgctgcagc
     3421 atcagtacgc tgcgttttcc agtccagcat ctggatttca attttcggcg caccgtgttt
     3481 acccacccag aaatcagcct ctggcgcaaa gggcaggaaa cctttggtgg taacgtacat
     3541 atcggagaac tgcataaagt tctcgccacc ggcattatcg ccaaaccagc cggtagagta
     3601 ctgctgacca acgttaccat ctatcatcac aacggcatca acccgtttgc cgttttcgtt
     3661 gtagacacgt tgttttaact gcaaatcaaa ccagccggag tattcgttac caaagcggcc
     3721 cagagaacca atcgcccatg atttaggtga accatgagag gcggtccccc aaccagaacg
     3781 gtaatagccg ttgtaactaa agccaatttc atctttcaca aacttactga aatctttcag
     3841 agtcatggaa gaataaatcg agcctgtctc actggcattt ttctcttcat ttttgaccag
     3901 tacagcatca ggttttgcgg cactactggt cgggaacgga ttggcggcat acccttgatc
     3961 attcgtactt acgctacgat tcaccgtcgc tggcgtatat tttttcttct cttcatcttt
     4021 atactttttg agttcgcttt gcgtttctct taacgctttt tctaataact caaggcgttg
     4081 ttccaccgtt aatgattcag ccaatgattg tgcagaaaag gctaacggtg ccattaataa
     4141 gatggcagag gtaataagat ttcgtctaaa cataatatcc ctttatggtg caaagaaaga
     4201 attaacgtat cgcatccaga ctgttctgaa tgcgacgata attaaggtgc tttattggtt
     4261 atttttttaa tgacagcccc cgcgttttga tcacttctgc ataccatccg aagcttttct
     4321 tacgcgttct tgtgaggctt ccttcgccat tatcatcacg atccacataa ataaagccgt
     4381 agcgcttaga catttgtgaa tgagatgcac tgactaaatc aattggcccc caactggtgt
     4441 accccataat atccacacca tcggcaatcg cttcatttac ctgtaccagg tgatcgttta
     4501 aataggcaat tcgataatcg tcctgtatcg aaccatccgc ttcaacgctg tcttttacgc
     4561 ctaatccatt ctcgacaata aataacggtt tttgataacg atcccaaagc gtatttaaca
     4621 gaacccgtaa tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat
     4681 tggggatcat attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa
     4741 cacaaccagt catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg
     4801 cgtcactttc agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc
     4861 cgggatattg gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca
     4921 tggcctgcaa catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca
     4981 tattgccgat tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg
     5041 caaccagttg atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc
     5101 ccacgcccgt gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg
     5161 ctactttatg ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga
     5221 ccgcccgatt agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg
     5281 ataacgttac cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa
     5341 acgctaaccc cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg
     5401 caatggaaat acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt
     5461 aacggtaata aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt
     5521 ccatttttcc cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt
     5581 ccagccaggc accttccacc tgattggcag ctgttgcacc accccaaaga aatgtttctg
     5641 gaaatgcttt cataattaac tccttttatc gttagcgaat gatggataac agcggttcac
     5701 ctgcgcttat ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga
     5761 ttaataccgg cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa
     5821 tcagccgatc gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc
     5881 cgtccagttt tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact
     5941 caatgccaat ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac
     6001 gcacttcacc aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg
     6061 tggtatcagc gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc
     6121 cctgctcagg tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct
     6181 gacgtttact ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa
     6241 tgacaccgcc aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct
     6301 gcatgaaggt gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa
     6361 tgatggtggc ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca
     6421 ggttgacgcc atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc
     6481 ccgccaccac ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga
     6541 cctgcgccat aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga
     6601 agttattgat acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc
     6661 agaagccgcc cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac
     6721 cggcggcaat catttcgctt atccaggttg atagcggccc caccagcaga aaggtgacgg
     6781 gtgtgataac catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc
     6841 acgcattaag tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa
     6901 ccgatgacga gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat
     6961 ccgctttttg cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa
     7021 tcaccatggc agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta
     7081 tcgggaagaa cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt
     7141 gttcggtcgt ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg
     7201 ccgccatcag accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta
     7261 gcagattacc tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca
     7321 cgctgttaac cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac
     7381 caccgctttc caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt
     7441 tgctttcatc ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt
     7501 tatctgcgcc cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc
     7561 taccgctttg cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa
     7621 gcaatttttt tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata
     7681 tttcagtgtt ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt
     7741 gaaattttac gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat
     7801 gcttgcgggt aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa
     7861 gcatgttcaa gaatacgcca agataaaaac ttcagatgtg taaccagtcg ctgataactc
     7921 aagctttctt cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc
     7981 attaactgcg tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga
     8041 tgcatggcaa taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata
     8101 atggttaacg cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac
     8161 agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata
     8221 taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag
     8281 gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca
     8341 tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt
     8401 ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc
     8461 accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt
     8521 ataaacaaaa aaaaccgact tcaccagtat tctctggtta tgtcaggttt tgcctgcgaa
     8581 tgcagtaaca atccagtcat ttattaatgg tttttataac gaacatccag gttcggaaat
     8641 taatttaatt gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat gaccatgctc
     8701 gcagttatta actttatgta attttaggaa tttataaagt tatatataac aaatcccaat
     8761 aattaagtta ttgggatttg tctggtgaat tatttgtcgc tatcttttcc cgccagcagt
     8821 ttatccagct catcaccgcc tacatgacgg aaatcctgac ccttcacgta gtagaagata
     8881 aactcacaaa tattctggca gcggtcgccg atacgttcga ttgaacgcgc acagaacagc
     8941 gcggtaagta cgctcggaat agtgcgcgaa tcttccatca tgtaggtcat cagttgacga
     9001 acaatacctt cgtattcctg atcgactttt ttatcttcac gataaatacg taccgcttcg
     9061 tcaatgtcca tccgcgcgaa cgcgtccagc acgtcgtgca gcatctggat ggtatgacgg
     9121 cccagcgact ccagacttac cagcaacggc tgatgctgct gggagaattt ctccagcgca
     9181 gtacggcaga ttttgtccgc cacgtcgcca atacgctcca gctcggcaat ggttttactg
     9241 atcaccataa ccagtcgcag gtcgctcgcc gtcggctgac gtttggcgat aatgcgcacg
     9301 caggcttcat cgatcgccac ttccatcatg ttgacgttct tgtcgccttc gatcacgcgc
     9361 ttcgccagat cgctgtcctg gttatgcatc gcggtgattg catcagaaag ctgctgctcc
     9421 accatgccgc ccatggtcat cacctgcgtg cggatacttt ccagttcggc gttgaactgg
     9481 ccggaaatat gtttattaag attgagactg tccataacgc actcctgaat caaccgtaac
     9541 gaccggtgat gtagtcttct gtttgtttct tcgctggctt ggtgaacaga tcgtccgtgt
     9601 tgctgaactc aatcaattcg cccaggtaca taaatgccgt gtggtcggaa caacgcgcag
     9661 cctgctgcat gttgtgggtg acgatcacca cggtgtaatc ctgcttcagt tcggtgatca
     9721 gctcttcaat acgcccggta gagatggggt cgagcgccga acacggttcg tcgagcagca
     9781 gcacttccgg gcgaatggcg ataccacgcg caatacacag acgctgttgc tgaccaccag
     9841 agagagagta accgctctgg tgcaatttat ctttggtttc gttccacaat gcggctttgg
     9901 tcaatgccca ctgcacgcgc tcgtccatgt cggcacagga gagcttctca aacagacgaa
     9961 cgccaaaggc gatgttgtcg tagatggaca tcgggaacgg cgtcggtttc tggaacacca
    10021 tgcccacttt cgcacgcagc agggcgatat cctgagagtt ggtcaggatg ttgtcgccat
    10081 caagcagaat ttcaccttcc gcacgctgct ccgggtacag ttcaaacatt ttgttgaagg
    10141 tacgcagcag cgtcgattta ccgcagccgg acggcccgat aaacgccgta acctggtttt
    10201 tggcgatatc caggttgatg tttttcaggg catggaattt gccgtagtag aagttcaaat
    10261 tacgaacctg aattttactc ggggcagttt caaccatact cattgcaatc ttttcctcat
    10321 cgtacctgat gcttttcgct aatcaggctt accgtagggc cgggtaaggc gttcacgccg
    10381 catccggcaa agtgttaacc gtgtttattc ttcgcaaaaa caacgcgcgc cagaatgttc
    10441 agcagcagga cgcacagggt aatgatcaat accccggccc aggccagttg ttgccattcc
    10501 gcaaacgggc tcatcgcaaa cttaaagatc gtcaccggca ggttggcgat cggctgcatc
    10561 atgtccgtgc tccagaactg gttggagagc gcggtaaaca gcagcggcgc ggtttcaccg
    10621 gcaatacggg caatcgccag caggataccg gtcataatcc cggacaccga cgctttcagc
    10681 gtaatcgcgg agatcatctt ccacttcggt gtacccagcg cataagccgc ttcacgcagg
    10741 ctgtccggca ccagtttcag catgttctca gtggtgcgga taacgatcgg cacctgcaac
    10801 agcgccagcg caattacgcc cgcccagccg gagaagtgct ccatctgtgc caccacgatg
    10861 gtgtagacaa acagaccaac cacaatcgac ggcgcggaga gcagaatgtc gttaatgaag
    10921 cgaatcactt ctgccagcca ggatttacga ccatattcca ccagataaat ccccgccata
    10981 atgcccagcg gcgtaccgaa taccgtggcc cacaaaatta acagcccgct acccgccagg
    11041 gcgttcgcca gaccaccacc ttccgtattg ggcggcggcg tcatttcagt gaacagcgcc
    11101 agcgacatac cgtcgatacc gcgagtgatg gtggacatta aaatccagat cagccagaac
    11161 aggccgaagg ccatcgtcgc catcgagagc gtcagcgcaa tacggttttt gaggcggcga
    11221 cgcgcctgca ttttgcggcg agattcagcc agcgccgcag tggtttgcat ttcaaccata
    11281 gccattagcg tgccccctca ttcttagcca ggcgcataat cataaacttc gatgcggcga
    11341 ggacgatgaa ggtaatcaca aacaggatca ggcccagttc catcagtgcg gcaacgtgca
    11401 gaccggattc cgcttccgca aattcgttcg ccagcgcaga ggtgatacta ttgcccggca
    11461 tgtacagcga ggcgctgtcg agctggtagg tgttaccgat gataaaggtc accgccatgg
    11521 tttcaccgag cgcgcggccc agccccagca tgatgccgcc gataacacca tttttggtga
    11581 acggaagaac gatacgccag ataacttccc aggtggtgca gccaataccg taggccgact
    11641 ctttcatcat caccggggtt tgttcgaaca catcacgcat taccgccgca atgtacggaa
    11701 taatcatgat ggcgaggatc acgcctgccg cgaggatacc gataccaaat gcggggccag
    11761 agaacagcgc gccaacaatc gggatattcg acatgatatt gccgaccggc tcctgaaagt
    11821 aaacggcgaa cagcggcgca aagataaaca ggccccacat gccgtaaacg atacttggaa
    11881 tggctgccag cagctcaatg gcgataccca gcgggcgttt cagccagcca ggcgcaagct
    11941 cagtcaggaa cagggcgata ccgaaactca ccgggacggc gatcagcagc gcgataaacg
    12001 aagtcaccaa cgtaccgtag atcggcacca gcgccccgta gatatcgttc ggtgcatccc
    12061 actctttggt ccataggaaa gccagaccaa atttctgaat gctcggccag gaggagatga
    12121 tcagagagac aataatgcca cccaacatca atagcacaat cagcgccgcc agttttacca
    12181 gcacgctgaa aattatgtcg ccctttttac ccggtgggtt aaaagcgggc ttggttgcag
    12241 ccataagtta ctcttcagtt aaacgcgttt acaaagtcat tttgtaatgc cggatgcggc
    12301 gtaaaacacc gtacccggcc tggagtttta ttagtacagc ggcttaccgc tactgtcttt
    12361 aatattggtc ttccacgcag cgcgaacctg ttcaactaca ctatccggca ggctggcgta
    12421 atccaggtcg ttcgcctgtt tagccccggt tttgtacgcc cagtcgaaga atttcagcac
    12481 ctctgtgcct tgttctggtt tcttctgatc tttgtggatc agaatgaacg tggtagaggt
    12541 aataggccat gcatcttcgc ctttctggtt ggtcagatcc tgagcgaagg ttttgctcca
    12601 gtctgcacct tttgctgcat tagcgaagtt ttcttcggtc ggactaaccg gtttaccatc
    12661 agcggagatc agtttggtgt acgccaggtt gttctgcttc gcgtaagcat attcaacata
    12721 accaattgca cccggcagac gctgaacgaa cgcggcgata ccgtcgttac ctttaccgcc
    12781 cagaccgatc ggccatttta cggtagagcc agtaccaacg ttgtttttcc actcttcgtt
    12841 cactttcgcc aggtagctgg tgaagacgaa ggaagtcccg gagccatctg cgcggcgtac
    12901 tacagcaatg ttttgtgaag gcagtttcag acccggattc agtttggcga tggcttcatc
    12961 atcccacttc ttgattttgc ccaggtagat gtcgccgagg gttttaccat ccagcaccag
    13021 ttcgccagac ttcagccctg gaatgttaac cgccagcacc acgccgccaa tcacggtcgg
    13081 gaactggaac agaccttcct gagccagttt ttcgtcagac agcggcgcgt cagaggcacc
    13141 aaaatcaacg gtattagcga taatctgttt tacgccaccg gaagaaccga taccctggta
    13201 gttaacttta ttaccggttt ctttctggta agtgtcagcc catttggcat acaccggcgc
    13261 agggaaggtt gcacctgcac ctgtcaggct tgcttctgca aacacagaga aagcactcat
    13321 cgataaggtc gcggcgacaa cagttgcgac ggtggtacgc ataactttca taatgtctcc
    13381 tgggaggatt cataaagcat tgtttgttgg ctacgagaag caaaatagga caaacaggtg
    13441 acagttatat ataaggaata tgacattttt atgacagaaa ggaattacca aataacttac
    13501 tgattttaaa tgtgaagatg aaaatcccac atgccatctt tttgtcatga tgcatttaaa
    13561 agtgataaag aaatgggtga gatgacaacg atgtgatgaa caatcgttgt cagacctatt
    13621 agcgcacaat ctcaacagtt atcgtcgctg ttgcagtaaa ttgccccggg gctgggcgag
    13681 ctccagtaag gctcgcagga gcagctgaaa aagttacact acctgatttt tcaccataca
    13741 ctgttgattt atccatgtct gttggcaact caccaccgtt cactttgatt tccttattat
    13801 ttttgtcgta aatttttatc cccacgttag agtttgaagt tacgaccatt gaattgtccg
    13861 tattgggttc ggtagcataa aaagacgtac tgatatcatc ggtattcagt atccccaggt
    13921 tttcacactc aatagtgact gttttagtaa ttttatgtgt tgttaccgca cttccaacaa
    13981 cagatgaaaa atctgcagct ggtatttctt taaaatcaaa acaaactggc tttaaattat
    14041 ttatttcgca actttgcgga gctgtaatat cgccccgaat ataaacatca gccatcggct
    14101 gtgagtaatc caccggggtg tcacggctaa ttgttccata caatgtcact atctttgttt
    14161 caggaataat caattttcct gcaaatgtct tcttgatata aaaatatact atcgctttag
    14221 ccccactgga tatacttaaa ggttcaatgc ggttgcaatt gtactttgta ttaccagacg
    14281 ggttgttagc atgatattca aaaggtgcat tcacataccc aaccccaaga atatctatag
    14341 caatcccaac atcaaggttc ggattaagat cataataatt aatattagag tgaaccccag
    14401 cataagttaa tgcctggttt atttttgacg tataatagat ataggggaat tctcctacgt
    14461 taccagtatc acaatgacag cgtccaagat atgaggatcc attagttaca gtggtgttaa
    14521 atgtcgttcc aaccttattt tcgttcgcgg taataagata tcccg
//
LOCUS       DAEEGA010000092        14558 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEEGA010000092 DAEEGA010000000
VERSION     DAEEGA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14558
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.092"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(337..582)
                     /locus_tag="KLH46_003208"
     CDS             complement(337..582)
                     /locus_tag="KLH46_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379802.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="KLH46_003209"
     tRNA            complement(1227..1302)
                     /locus_tag="KLH46_003209"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="KLH46_003210"
     CDS             complement(1408..2115)
                     /locus_tag="KLH46_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBE0379803.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="KLH46_003211"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="KLH46_003211"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBE0379804.1"
                     /translation="MKSMNIAASSEVVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="KLH46_003212"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="KLH46_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBE0379805.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="KLH46_003213"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="KLH46_003213"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBE0379806.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="KLH46_003214"
     CDS             complement(7299..7574)
                     /locus_tag="KLH46_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBE0379807.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="KLH46_003215"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="KLH46_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBE0379808.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="KLH46_003216"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="KLH46_003216"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBE0379809.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="KLH46_003217"
     CDS             9534..9860
                     /locus_tag="KLH46_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBE0379810.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="KLH46_003218"
     CDS             10044..10763
                     /locus_tag="KLH46_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBE0379811.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="KLH46_003219"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="KLH46_003219"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBE0379812.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13063
                     /locus_tag="KLH46_003220"
                     /pseudo
     CDS             12102..13063
                     /locus_tag="KLH46_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
     gene            complement(13164..14477)
                     /locus_tag="KLH46_003221"
     CDS             complement(13164..14477)
                     /locus_tag="KLH46_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBE0379813.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPK
                     LILESVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLL
                     IVGIFMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGC
                     SISGVKIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3812 a         3486 c         3741 g         3519 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct atttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg atcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaagtggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctgacgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctgtg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac atcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agcagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaataaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat gcaaagctaa gggataatgc gtagcgtcca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgatta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacttggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat
    13021 tttgatgaaa attgacgata tcaaccaagc taatgaacgc tagaaaaaag ataccgaaca
    13081 actgcggcgc aatggggtga ttaagtaact catgttaagt gcctttgccg gataatataa
    13141 aaagttatcc ggcaaattat tttttacatt agccctagta gctgtggaat gaacaaggat
    13201 atttgcggaa tgtaagtgat catcatcaac gctactagca atgcagcata aaatagcagc
    13261 aatggtttga ttaaatgttg aatcttcaca cccgagatag aacaaccaac aaatagcgca
    13321 ctacccaccg gcggtgttaa cagcccaata caaaggttgg ccaccatcat aataccgaaa
    13381 tgtactgggt ccattcccag ctcttgtgca atcggcaaga agatcggtgt aaagattagt
    13441 acagccggtg tcatgtccat aaagatgccg acaattaata agacgatatt aatcagcagc
    13501 aatatgatca gtgggttatc agaaatcccc atcaatgcgt cgctgatcat ataaggaata
    13561 tcagcattag tcattgccca ggacatgccc acggaaaaac cgatcaacag taatacaatc
    13621 gaagtggtaa caaccgactc cagaatcagt ttgggtaaat ggcgccattt tacttcccgg
    13681 taaatcagca ccgagagaat aaatgtgtag acaacggcaa ttgctgacgc ttcagttgcg
    13741 gtaaaaatgc cgcccagaat gccgcccaga atgccgccca tcacaatgaa caccagaagc
    13801 aaacttggta aggcatccag aaacgcttta caggcctgag caaaggttgc tctttctgaa
    13861 agcgggtagc cacggcgttt agcaataatg ccgcagacta tcattaccgc aagacccatt
    13921 aagatccctg gcaaatatcc cgccataaac agcgatgcta cagaaacgcc gccagctgtc
    13981 agggaaaaca caattaacac gtttgagggc ggtatcagta atccagtaat acaagatgag
    14041 acgtttacag cggtagaaaa tgccggatca taaccttctt tggtctgaat gggattcagt
    14101 gtgccaccaa cggcagcagc tgccgcaacg gctgaaccag aaatggaacc aaacatcata
    14161 ttcgccagga cgttcacatg accaagcgaa ccaggaacgc ggccaaccat tacctgggcg
    14221 aggttaatca aacgtatcgc aataccgccg ctgttcatca gtgttccggc aaagatgaaa
    14281 aaggggattg ccaacagggc aaagttatcc agtccattag ccagtcgttg cgaaacagtg
    14341 attgccgcga tatcccaggg aaacatcaaa agcattgaag ctacagtggc aataccgatt
    14401 gagaacgaaa tgggcacacc aataaagacc agcacaaaaa aacagccaaa cagtgtaatc
    14461 gcgatataac tatccattgg taatgctgcc aacttactga tttagtgtat gatggtgatt
    14521 ttaaggtgct tgcgtggctt ccatttccat cagatgtc
//
LOCUS       DAEEGA010000093        14443 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEEGA010000093 DAEEGA010000000
VERSION     DAEEGA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14443
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.093"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(113..379)
                     /locus_tag="KLH46_003222"
     CDS             complement(113..379)
                     /locus_tag="KLH46_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBE0379814.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="KLH46_003223"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="KLH46_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBE0379815.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="KLH46_003224"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="KLH46_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="KLH46_003225"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="KLH46_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBE0379816.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="KLH46_003226"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="KLH46_003226"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBE0379817.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7452..8819)
                     /gene="dbpA"
                     /locus_tag="KLH46_003227"
     CDS             complement(7452..8819)
                     /gene="dbpA"
                     /locus_tag="KLH46_003227"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBE0379818.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9297..10280)
                     /gene="zntB"
                     /locus_tag="KLH46_003228"
     CDS             complement(9297..10280)
                     /gene="zntB"
                     /locus_tag="KLH46_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBE0379819.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10535..11767
                     /gene="dgcM"
                     /locus_tag="KLH46_003229"
                     /pseudo
     CDS             10535..11767
                     /gene="dgcM"
                     /locus_tag="KLH46_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11788..12351)
                     /gene="smrA"
                     /locus_tag="KLH46_003230"
     CDS             complement(11788..12351)
                     /gene="smrA"
                     /locus_tag="KLH46_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBE0379820.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12605..14059)
                     /locus_tag="KLH46_003231"
     CDS             complement(12605..14059)
                     /locus_tag="KLH46_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HBE0379821.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14056..>14443)
                     /locus_tag="KLH46_003232"
     CDS             complement(14056..>14443)
                     /locus_tag="KLH46_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE0379822.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3853 a         3638 c         3410 g         3542 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgta atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaactgctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caagatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagatgt ttactcttgc acccggcaat
     7441 tcaacatttc attattttaa taaccgcacc cggcacgttt ttcctttaat cttcccgccc
     7501 tgtaactgtt tccatgcttt atgagcaaca gcctgacgga ccgcgacata gacatgcgcc
     7561 ggatgcacgg cgattttgcc aatatctgcg ccatcaagcc cgatatctcc tgtcagtgca
     7621 cctaatacat cacccgggcg cattttggct tttttcccgc catcgataca caacgttgcc
     7681 atttctgctt ccagcgtcac aatggaacta ttagctggcg gcgtttgcca gttaagtttt
     7741 atctgcaaca tgtcagaaat gatattggcc cgctgtgctt cttccggagc acagaaactg
     7801 atcgccagac cgctatttcc tgcacgagct gtacgaccga tgcgatgtac atgaacttca
     7861 gggtcccacg ccagctcaaa gttcaccacc agctcaagcg atttaatatc cagaccacgc
     7921 gcagcaacat cagtcgcaac cagtacacgg gcgctaccgt tagcaaaacg taccagggtc
     7981 tgatcgcgtt gctccagatc gccgtgtaac gacaatgcac tttgctctac ttcattcagc
     8041 gcgtcgcaga cagcctggca atcttttttg gtattgcaaa acaccacaca agaggatggc
     8101 tgatgcaagc ttaataaccg ttgcaacaga ggaattttgc ctttactgga tgtctcataa
     8161 aattgttgtt caatgggtgg caaagcatct gttgagtcaa tttcaatcgc caaaggatcg
     8221 cgttgcactc gtccgctgat tgcggcgatg gcttccggcc aggttgccga aaacagaagc
     8281 gtctgtcgag atgcaggcgc aaaacggatg acatcatcaa tggcatcgct aaatcccata
     8341 tccagcatgc ggtcggcctc atccatcacc aaagtattca gcgcatccag tgataccgtg
     8401 cctttttgca agtgatccag caaacgaccc ggcgttgcca cgataatatg cggcgcatgt
     8461 tgcagcgaat cacgctgcat accgaacggt tgaccaccgc acaacgtcaa aattttggta
     8521 tttggcagaa aacgcgccag ccgacgcaat tcacctgcca cctgatccgc cagttcacgc
     8581 gtaggacaca gcactaaagc ctgggtttga aatagcgacg catcaatttg ctgtaacaag
     8641 ccgaggccaa aagccgccgt tttgccgctg ccggttttcg cctgcacgcg aacatctttt
     8701 ccggcaagga tcgccggaag cgcggcggcc tgcaccggcg tcatggttaa ataacccaac
     8761 tcattaaggt tcgtgagttg ggcgggaggc aaaacattca gggtagaaaa agcggtcaca
     8821 atctattctc gtggtcatcg acgcaaagtt agcaggcgcg tatcctcgca gatctacgct
     8881 cacgatgcga caatttaatc ggttcttcat cgggtggtgg gtcaggcatg ggttgcgggc
     8941 gagggatcgg atcgggcact ggaacaggat cgccaggaat cggttcaggg acaggaattt
     9001 gcaaataaat aagtgtcgtc atatttccct ccggtcattg ggtggactct taaagggtag
     9061 acgctgataa ataacaggca aaaaaaagcc gactcatcaa agtcggcgtc gtacgaatca
     9121 attgtgctat gcagtaattc aaaaaaggaa gtaagacaat atggagcgca acgcccatcg
     9181 cttgacgttg cattcacctg caagggagat attgccctga atgggtagag agtttattga
     9241 cttcgctcaa actttgcggc gtttttgtat acagacagcc ggaaaaattg cttttgttac
     9301 aaccatttac tacggtgcaa ccataaagca acaccaccaa taagaacaac taacagaata
     9361 caaaaaattg aaaatccgaa ttgccacccg ccgccaggga tcccaccaag gttgacgcca
     9421 aataacccgg tcagaaaggt actgggtaaa aagaccattg ccatcaacga cattgtatag
     9481 gtacgacgag ctaaattttc ctgcatcacc tgagcgattt catccgccat cacgccagtc
     9541 cgtgctatac aggcgtcgat ttcgtcaagg ccgcgcccaa ggcgatcggc aatatcctgc
     9601 atccgacggc gttggtcatc gctcatccac ggcaaacgtt cactggcaag acgagcataa
     9661 acatcacgtt gcggtgccat atagcgacgc atcacaatta attgtttgcg cagcagagcc
     9721 aggaatccac gcggtggaat ttgctgatca aggagattat cttcaaggtc gataatttta
     9781 tcgtgcagct gctcgataaa ttcactggaa tgatcggtca acgcatcgca cacatccacc
     9841 agccatcccc cgcaatcggt cggacccgtg ccctcttcca gatcgctcac cacatcgtcc
     9901 agcgccagca ctttgcgttg tcgggtcgaa acaattaacc gcccgtccat atatacacgc
     9961 atggcgacca gttgatcggg gcgttcatcg gtgctgccgt ttatacagcg caatgtaatc
    10021 agcgtgcctt caccgaaacg gctgactcgg ggccgtgtgc tctcgcccgc cagcgcatca
    10081 cgtacgttat tgggaagcag cggtgttgtc gccagccatt gggcgctatc atggtgtaca
    10141 taattaaggt ggagccagca gggatgcgct tcatcaatca catctgtatt ttccagcggt
    10201 ttaacgccgc ctctaccatc cagcatccag gcaaatactg catccgggac attaacgtcc
    10261 gatcccttaa tcgcttccac agtgcctcca tcatcaacgc attattttgt agtctagcct
    10321 tctggccctg ttacgcaaca tctcatcacc ccattaccct gaaatgatta ataaaattct
    10381 gtctaaattg aatacaaaaa gcaaaatgct tttccgtata caaaccgtgt gaagtgttaa
    10441 atagcgtcta tcattatcag aattatctga tcatatgacg tggctttttt gcgatcggat
    10501 agcaacaaaa attgataaaa ataacgggat ctcaatgatt acgcacaact tcaataccct
    10561 ggacttactc accagtcctg tctggattgt ttcgcccttt gaggaacagt taatttatgc
    10621 caatagcgcg gcgcgactgt tgatgcaaga cctcacgttt agtcagctac gaaccggacc
    10681 ctattccgtc tcctcacaaa aagaactgcc gaaatacctc tccgatctgc aaaaccaaca
    10741 cgatattatc gaaatcctca ctgttcagcg taaagaagag gaaacagcat taagctgtcg
    10801 gcttgttttg cgagagctga cagaaacaga accggtgatt attttcgaag gtatcgaagc
    10861 gccggcaacg ctgggtttaa aagccagtcg ctcggcaaat tatcagcgca aaaaacaagg
    10921 tttttatgcg cgcttttttc tgactaactc tgcaccaatg ttgttgattg acccgtcacg
    10981 agatggacaa atcgtcgatg ctaacctcgc cgcgctcaat ttctatggtt ataaccatga
    11041 aacgatgtgc cagaaacata cctaggaaat aaatatgctt gggcgtcgcg tcatgcctat
    11101 catgcatgaa atttcgcatt tacccggcgg tcataaccct ttgaattttg ttcataaact
    11161 ggcggatgga tctactcgtc atgtgcagac ctatgccgga ccgattgaaa tttatggcga
    11221 caagctcatg ttatgtattg tgcatgatat tactgagcaa aaacggctgg aggagcagct
    11281 ggaacatgct gctcaccatg acgcgatgac cggattactg aatcggcgac agttttatca
    11341 cattacggaa ccaggccaaa tgcagcatct cgccatcgct caggattaca gcttgttgct
    11401 catcgacacc gatcgtttta aacacattaa cgatctctat gggcattcta aaggtgatga
    11461 ggtgttatgc gccctcgccc gccccctcga aagttgcgct cgcaaaggcg atttggtgtt
    11521 tcgttgggga ggcgaagagt ttgtcttatt gctaccaaga accccactgg ataccgcgct
    11581 ttcgctggct gaaactatcc gcgtaagcgt ggcaaaagtg agtatttcgg gcttaccacg
    11641 ctttaccgtc agcattggtg tggcgcatca cgaaggaaat gaaagcatcg atgaactgtt
    11701 taaacgcgtt gatgatgctt tgtatcgggc gaaaaatgat ggacgcaacc gcgtgctggc
    11761 ggcataagcc gcggatgcgt ctcgagatca acgactgcgc ttagcgtggc gctcccagtt
    11821 ttcttgcttc gcctgcgccg ttttacgtag tgcgacgtaa cacgccccgc tgccgccatg
    11881 atgcggtagc gcggtgcaat atgcctgaac atcatcaaat tcggtcagcc agcgcgccac
    11941 atagctgcgg acaatattgg catgtgattt atcttcccgc cctttaccat gaataatcag
    12001 cacattacgc aaaccatccg ccagggcttg ttgaatgaaa ctgaacacca ttttgcggca
    12061 ctcttccacc ggctggcgca aaagattcag gctcgcctgt tgcggatatt taccactgcg
    12121 cagcttatcc agcaccccat gctgcaaccc ttcctgccga aactccagcg gctgacttag
    12181 cgggatgatg tcgagaaatc cggtagtgag gaaattatca agctgcagcg tgtcgatacg
    12241 ctgcggcgca cgttggttac gcgttggatg ccagtggaca tctgtagcac gtttcagcgg
    12301 ctggacatct tccatggcgt caagaaacag cgatttgtcg tcaaggttca tgtaacctcc
    12361 tatgcacctt cacacgtcgt gattcagacg ggttaattgt tcatacttac tgcatcgttc
    12421 ccttcacaga ttgaaacaat actctacttc atacctgtga gtatctgcgc aggcggggca
    12481 ttttatacta gcctgttagc agtatcgaac agtttagccc gagtttaatt cagcacaggc
    12541 tccgatcagg atgatttatt catagcagga accaacaaaa atgcgataaa taacagacca
    12601 ctgattagca gaaaggtacc gaaaatgcca tagtttgcgc ctccctcagc atcaattgac
    12661 tcaataattt ggttgaagcc agtatttccg atgtctggtc acttctactg cgattaaaaa
    12721 ataaagcact tctggaaatg aagtcattca ccgtatgggg aacagaaacc attttttcac
    12781 ccattctcga aaaatgaaaa ttagttcgat tattgagaat agagccacac agggcaatac
    12841 caatcgcgcc cccaagatta cgtgttaaat taaatacacc agaggctaac ttcagccgtt
    12901 cttttggaat tccccccagc gttaatgtta cgatgggggc catcgcaaac tgctgcgaaa
    12961 tccctctaat tgcctgtgga aacagtaatt cctgccatcc ccattcgtgg gttatagggg
    13021 taaaaagata cattgaaaac acaaatccgc ccagccccgc catcaacagc catcgcagat
    13081 ttattttttt acttaaccag aagtaaaagg gaacagaaaa taactggaaa atacctgttg
    13141 tgcaaacggc aaaacctatc tcttcggcat ttaatccgcg aatctgcccc aaaaacacag
    13201 gtattaaata tacagtagag aatatcccaa cccctccaga aaaagaaaaa aaacatccca
    13261 gagtgaaatt tttatcctta aaagcatgaa gatccattat cggattggat attgtaagtg
    13321 tacgcgcagc aaataaaata aaactcacca atgccagaac tgatgtcagc aaaatagtat
    13381 tatcatccag ccatccccag cgcgctcctt cttcaagggt atattccaga catcccagag
    13441 tcatggccaa caggataata ctcggataat cagcaacttt cagtaaagag agatcaggtt
    13501 tatcaaaatt aaccagaaaa ggaatactca aaactaaata tatgccaggt aatatattga
    13561 tataaaatag ccatcgccag tcgaggttat cagtgagcca gccccccaga gttggcccta
    13621 gcgtcggtga cagtgaggcc agcgcactga ctaccgcagc agccaacccc aactctttac
    13681 cctggtaata aataaacgcc atcgtgaaaa ctaaagggat catggatgcg ccagcagctc
    13741 cctgtaaagc acgaaacaaa atcatgatct gaatattcca tgcaagtcca cacgcgatac
    13801 tcattaaagt aaatattccc gcggatagag taaaaagcca acgagtggaa aatacccgtg
    13861 acagccaccc agagagcgga ataacaataa tctcagcaat taaatagctg gtctgaagcc
    13921 aggccatttc gtctttacct gcggacagac caccacctat ttcatttagg gatgacgata
    13981 caatttgaat atcaagctga gcaataaata atcccataca catactggcg aaagcaatat
    14041 gtttacgcat agacatcatc tctcatcaac actgacggtg acggacagtc ccgggcgaag
    14101 aagttgcttt aattcctttg cgtcgtcaaa tgcaatccgt accgggacac gctgaacaat
    14161 tttggtgaaa ttgccagtgg cattttctat cggaatcaag ctgaaactgg ctcctgtcgc
    14221 tggggaaagg ctttcaatgt gaccgtgaaa cacttcccct ttgagaatat cagcacggat
    14281 ctcagccttc attcctggct tcattcccgc tatttggttt tctttataat ttgcatcaac
    14341 ccacaactca ctgactggta ctagtgaaac taacgatgtg ccgccttcca cccaactgcc
    14401 tgtatgggcg ctgcggtttg cgacaatgcc atcaatcggc gca
//
LOCUS       DAEEGA010000094        14253 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEEGA010000094 DAEEGA010000000
VERSION     DAEEGA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14253
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.094"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..107)
                     /locus_tag="KLH46_003233"
     CDS             complement(<1..107)
                     /locus_tag="KLH46_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HBE0379823.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGG"
     gene            complement(148..603)
                     /gene="csgB"
                     /locus_tag="KLH46_003234"
     CDS             complement(148..603)
                     /gene="csgB"
                     /locus_tag="KLH46_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBE0379824.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1356..2006
                     /gene="csgD"
                     /locus_tag="KLH46_003235"
     CDS             1356..2006
                     /gene="csgD"
                     /locus_tag="KLH46_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBE0379825.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2011..2400
                     /gene="csgE"
                     /locus_tag="KLH46_003236"
     CDS             2011..2400
                     /gene="csgE"
                     /locus_tag="KLH46_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBE0379826.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2425..2841
                     /gene="csgF"
                     /locus_tag="KLH46_003237"
     CDS             2425..2841
                     /gene="csgF"
                     /locus_tag="KLH46_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBE0379827.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2868..3574
                     /locus_tag="KLH46_003238"
                     /pseudo
     CDS             2868..3574
                     /locus_tag="KLH46_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3707..4198)
                     /locus_tag="KLH46_003239"
     CDS             complement(3707..4198)
                     /locus_tag="KLH46_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBE0379828.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4300..4854)
                     /gene="ycdY"
                     /locus_tag="KLH46_003240"
     CDS             complement(4300..4854)
                     /gene="ycdY"
                     /locus_tag="KLH46_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBE0379829.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4878..5615)
                     /gene="ycdX"
                     /locus_tag="KLH46_003241"
     CDS             complement(4878..5615)
                     /gene="ycdX"
                     /locus_tag="KLH46_003241"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBE0379830.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="KLH46_003242"
                     /pseudo
     CDS             complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="KLH46_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6843..6930
                     /locus_tag="KLH46_003243"
     tRNA            6843..6930
                     /locus_tag="KLH46_003243"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6877..6879,aa:Ser,seq:gga)
     gene            complement(7080..7361)
                     /locus_tag="KLH46_003244"
                     /pseudo
     CDS             complement(7080..7361)
                     /locus_tag="KLH46_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7643..8647)
                     /locus_tag="KLH46_003245"
     CDS             complement(7643..8647)
                     /locus_tag="KLH46_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HBE0379831.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8719..9279)
                     /locus_tag="KLH46_003246"
                     /pseudo
     CDS             complement(8719..9279)
                     /locus_tag="KLH46_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9364..9561)
                     /locus_tag="KLH46_003247"
     CDS             complement(9364..9561)
                     /locus_tag="KLH46_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379832.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9573..10064)
                     /locus_tag="KLH46_003248"
     CDS             complement(9573..10064)
                     /locus_tag="KLH46_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379833.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10061..10435)
                     /locus_tag="KLH46_003249"
     CDS             complement(10061..10435)
                     /locus_tag="KLH46_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBE0379834.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10482..10856)
                     /locus_tag="KLH46_003250"
     CDS             complement(10482..10856)
                     /locus_tag="KLH46_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBE0379835.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11019..11240)
                     /locus_tag="KLH46_003251"
     CDS             complement(11019..11240)
                     /locus_tag="KLH46_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBE0379836.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11303..11779)
                     /locus_tag="KLH46_003252"
     CDS             complement(11303..11779)
                     /locus_tag="KLH46_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBE0379837.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11795..12268)
                     /locus_tag="KLH46_003253"
     CDS             complement(11795..12268)
                     /locus_tag="KLH46_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBE0379838.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12610..13431)
                     /locus_tag="KLH46_003254"
     CDS             complement(12610..13431)
                     /locus_tag="KLH46_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBE0379839.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13531..13704)
                     /locus_tag="KLH46_003255"
     CDS             complement(13531..13704)
                     /locus_tag="KLH46_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBE0379840.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13824..>14253)
                     /locus_tag="KLH46_003256"
     CDS             complement(13824..>14253)
                     /locus_tag="KLH46_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379841.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3641 a         3491 c         3413 g         3708 t
ORIGIN      
        1 ccgccaccac cgtggttacc gccgccgccg tactgaggaa caacacctgc cagagcgcta
       61 ccggagaata cgattgctgc aattgctgct acttttaaaa gtttcatgta aaacccccat
      121 cggattgatt taaaagtcga atggtaatta acgttgtgtc acgcgaattg ccatttgcga
      181 ctgtctctgc actacaattg ccgttttttg agtaccatac tgtgtaatat ttgctttatt
      241 accagaacct ttctggataa tcatcgcagt attaccataa gcaccttgcg aaatactggc
      301 atcgttggca ctgcccgcct gatcaatata tgcaaggtta taatctcctg tctggtcaat
      361 ctttgcccga ttgctactac cttcttgcgc aacaaccgcc aaaagttttg agcctccctg
      421 ccgtaactga gcactattat tagtcccagc ttgaccaatt atggctgcct gattaaatga
      481 agacttactc aattcattta ccgcgaagtt atattctgaa ttagctaaat cataacctgc
      541 tgcggctgca atcccaggcg cacccagtat tgttaacatc ataaataaca atttgttttt
      601 catgttgtca ccctggacct ggtcgtacat ttaagaaatt aaatcatttc aacttggttg
      661 ttaacgcaac ctgtattttg ttaacgctgc gttacgatgg aaagtatgtc tgcggaaata
      721 tttttaataa ctcacccgcg cgttgtattt ttcttttttt gttcatacca aagtattaaa
      781 acccgcgtaa atacgctgtt atctacgcaa aaaatatttt gttttctctt aaatctccgt
      841 tttccgctat caaaaatcac cagacagtca ttcttcttgc ccgtcgctga ttgctgcgat
      901 atgtcttgcg cacaagccgt gacaaacctc gtctttttgc agtaaaaaaa ttgtccacgg
      961 aggtgtggag aaaaaacaaa aacgttttac atgacgaaag gactacaccg aaatattttt
     1021 atatgtatta ttagtaagtt agcaccattt gtatgatttt ttaaaattgt gcaataaaaa
     1081 ccaaatgtac aacttttcta tcatttctaa acttaataaa accttaatgt taacatttta
     1141 atataatgag ttacattttg ttacatgttt aacacttgat ttaagatttg taatggctag
     1201 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
     1261 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
     1321 caggtgtgcg atcaataaaa aagtggggtt tcatcatgtt taatgaagtc catagtattc
     1381 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
     1441 accttaaaca atcgctagca attacaggaa aattacataa tattcaacgt tctctggacg
     1501 atatctcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
     1561 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
     1621 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
     1681 atgccatgga ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgtggcgaat
     1741 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
     1801 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
     1861 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
     1921 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
     1981 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
     2041 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
     2101 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
     2161 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
     2221 agctggatca caataacggt caatcaggat gttattttcc agactttttt attcccgttg
     2281 aaaagagact tcgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     2341 cgtcgccaga taaatcaggc gttattaagt acggacgatt tggcgcatga tgaattctaa
     2401 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     2461 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     2521 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     2581 gatccgagct ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact
     2641 caggccatcc agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg
     2701 ggccgcatgg tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag
     2761 ttgaacgtga cagatcgtaa aaccggacaa acctcgacca tccaggtttc gggtttacaa
     2821 aataactcaa ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat
     2881 ttcttttggt tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg
     2941 ccagaccgac attaatgcct cgtgctcaga gctacaaaga tttgacccat ctgccagcgc
     3001 cgacgggtaa gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac
     3061 cctacccggc aagtaacttc tccactgctg ttccgcaaag cgccagggct tacaaaacct
     3121 gcttaacgag cgcaaggtta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa
     3181 ccgaatcccg ctgcaatctt taacggcggc aaatattatg gttgaaggtt cgattatcgg
     3241 ttatgaaagc aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga
     3301 cacgcaatac cagctcgatc agattgccgt gaacctgcgt gtcgtcaatg tgagtaccgg
     3361 tgagatcctc tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg
     3421 ggttttccgc tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa
     3481 cgaacccgtt atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa
     3541 tgatggtatc gaccgtggtc tgtgggattt gtaaaataaa gcagaacggc agaatgacat
     3601 tctggtgaaa taccgccata tgtcggttcc accggaatcc tgacagataa aaatgccgga
     3661 attaataatc cggcatttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt
     3721 tgatttcctg ctcacggtga gccgtctttg cactacgcgc cgccagccac aggccgctgt
     3781 ttttcattgc gtaaccaaat accagcccaa gcgccagcga aggtaacacc agtttccagt
     3841 caccctgccc ggcaaacgtc gcgcatgcgc ctataaacgt ccccggaaca aatgaaagca
     3901 gcagctgttt ggcctgaata cacatcagaa acgcgacaat accggttatg acataaccga
     3961 gaatttccag atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc
     4021 cactaagcag ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaagcaa
     4081 agtaggccgt acagcccagg aagcccgccc agcttagtaa gcccagggaa acagccaccc
     4141 atccccagat accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc
     4201 cgcgcatgat agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa
     4261 caaattatct gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc
     4321 cagctcgtcc cacattgcac taatggcatc acgggttagc ggtgccatgg tgcgccagaa
     4381 aggcgtggtt gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata
     4441 ctcactgaac agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca
     4501 ggaagccgca agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtggcatccc
     4561 tcgttcggaa agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg
     4621 atatggcggc acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg
     4681 cgtcatatca cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt
     4741 cgcagccagt ttcccctcac gaatcagagt aaacagcggc accagtaaag gatcttgcgg
     4801 ttggcggtaa tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat
     4861 atttccatta ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat
     4921 tcaaggaagt tcagtaagcg gcgcggagaa acattcaaaa tgcgctctag cggaaaatct
     4981 accgcgtcga ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa
     5041 tccgagccta atgccaccca acctccagca tcacgtaccg ctgcagctac cgcacgacag
     5101 ttgtcttcac tgcccttacg tgagtgtaaa aatgaggaat tattgatttc cagcgccacc
     5161 tgatgtttcg cggctgcttc agcaacggct ttaacatcta tttcatattt cggatttccg
     5221 ggatggctta ttatatgcac attgccgctg gcgatagtgg agatcatcgc ttgtgtattg
     5281 gtcgctttgt catgtggcgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc
     5341 gagtcaaaca ttttaccgct gcagtcaatt tcaccatcaa catttttaat gttagcttca
     5401 atgccgcgca ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag
     5461 tgccagtgat gcggcgcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta
     5521 atgccctttt gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg
     5581 gcaacggtat gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg
     5641 ccaaggatag caggaatcct gatgctttat tagtagccac gtgcgcggtc gacttgcccg
     5701 cagaccctct cccctttttc gagctgggca atagtgcgag aaatgtactc cacagcttca
     5761 gcgggacggg taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc
     5821 ggactttcag gcggtaaggg ttcacgatta aaaacatcca gcattgcgcc tttaactttc
     5881 ccgctatcca gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg
     5941 aggagatacg cgccatcagg taatttttcg agtaattgtt gattaataat gccgacggtt
     6001 tcaggggtat tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt
     6061 tcttcccgtc cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa
     6121 cgcagcggaa agcgctaggt ttgcagactc tgagcaactt tacagcccag tacgcctgcg
     6181 cccaaaatgc cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa
     6241 ctattttgct ggatgcgata atcgtcaaaa cgtcgaaacc aatgcagcac ctgactgaca
     6301 gcatattcct gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa
     6361 gggttcagca tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg
     6421 agtgcgaaca ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact
     6481 aaaacataat cagcagagtc attatctccg cttttccatg ccctgactct tgcctgagga
     6541 atagctttgc gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg
     6601 atatccatac tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat
     6661 ctacaaaggc caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt
     6721 attaaaacca cacattgatt gaatgtttga ataaacgcgc gattttttca aaaagtttgt
     6781 tgacctcagg tcatgatttc cctaaattag cgcccgttcc agcaagacag gaacgacaat
     6841 ttggtgaggt gtccgagtgg ctgaaggagc acgcctggaa agtgtgtata cggcaacgta
     6901 tcgggggttc gaatcccccc ctcaccgcca gattataagt gcatgttatt gtttaacatg
     6961 cgctttttta tttttccttc cagacagtat gccatcgggt atgccatctg aaaccggttt
     7021 atatgccccg cgatcatatc ttatcggaca atttccgatc tttgagcata ctggcaaagc
     7081 tatactgcca gcatatcagg atagtgtttt gcgataatat cattcagtct gtcaaccagt
     7141 tctggttttc tgaacgtgat atgcgcggag cccttcttaa agtatctgat gctgaacatc
     7201 tcatcttcat agctctcttt accctgaaca gactgaatgt gatcatccag acgaagggtg
     7261 atgtttcccc ggttgtcggg gattggttta ccgctgaaca ggtgcaacat tctttccaga
     7321 tcggcgagtc ggtcagcctg ttgtccggtg ataagatgaa atctatggtc acccccgttt
     7381 ttgcaacact gatttttgat taatggttgg cttgcttaaa tctatccggc gtctctgtgg
     7441 gcaaggatgc ccgcgccccg atgagttccg cacctgataa tcctaaaaat cccctcggct
     7501 tcagtgagcc atttcatctg ttaggttttt aatcaggtcg atttgccgtt catgtcatca
     7561 ataatcagtc ttcgcaaaac cagaaaggta acttgtatct tattgtttaa aataaactaa
     7621 ttgagttata tcagcatcta tatcaggcct gatattcttt tttctgagta gtgattgccc
     7681 aggcaattcg agccagttta tttgccagag cgcaggtaac cacattcgaa tgcttcttgt
     7741 tgagttgctc ccgaacccac tcagcaagtt ttccatgctt gcgttcaagt cgcattatga
     7801 atgagcgggc acactgaacc agtaaccggc gtaaattttt atcacctcgc ttactgatac
     7861 ccaacagagt cgattttcct ccagtgctat actgccgggg aacaagtcct gtggaagctg
     7921 caaaatccct gctgcatgag aactgtttcc cgtcaccaag ttgcgacgat agtaagctgg
     7981 cagtaatcgg accaacacca ggaattgtca taatgcgctg agcggtatca tcagcgttga
     8041 ttgactgact taattctgat tccagctctg ctatttgctc aacaaggtaa aagtagtggg
     8101 tatgcaacct caccaaaagg cggctcagat attcggggat ttcgtgttca gccaacacga
     8161 gagacaaccg ttttattaca gcatcaccta tcggcaggct gattccaaat tccagcaaga
     8221 aaccgtggat ctggttggtt gttttcacct tatctctgat gagcgattct ctgacccggt
     8281 gtaacgccct catggcctgc tgggtctcag ttcttggctg gacaaaacgc attgatggtc
     8341 gtgaagccgc ttcgcatatg gcttctgcat caacaaaatc attcttatta cttttgacaa
     8401 acgggcgaac gaactgtgga gaaatcagct tagtttcatg cccaaaatca gagatacagc
     8461 gagccataaa gtgtgcaccc gcacaggctt ccattacaac aactgagggc ggacatgtcg
     8521 ccagaaactg catcagttta gtacgggtaa attttttacg cagaagagcc ttaccagact
     8581 tgtcttgaca atgaatgtgg aaggagtgct ttccgagatc gataccaata agtgtgatgt
     8641 tctgcatgat ggtctctccg ttaaagggat ttaccttaac agcgtactgc tgttaaggtg
     8701 ggggtgacca tatcattatc cccaccggtc ccatctcacc agattgttga cgataatttt
     8761 actgccaaat ttacaggggc aattggtttt gtaattccag ttcagccctc tgaagacgtt
     8821 gatcacccct cgctcaaaca cctcatcctt attctggtgc agttgttcaa atgtgctcag
     8881 aatgttcgct tcgctgattt ccggaaaatt atcgtactcc agtgaccggt accatgtatc
     8941 acgggcctgc gtatccatta atgacagcat cccggaacgt tgcatcaggt cacgccagat
     9001 ttcgcgatca agattccggg taatggcttt cattgctgtt tcgggtttct ccatcagcca
     9061 gcagccgcag cgaaaatcct gtttcatggc ccagtccggg gcagttttac ctccgatgct
     9121 ttccgtcagc accgagattt cagccagttg tcggataagt gtgtcgattt gcaacagtgt
     9181 actctttcgt acagccagaa tgtggcgaat gctggttgag cagataacgt ccgtatgccc
     9241 gttgaccact tccggacggg agattgtgat atctgacata atcgatattc ctttttgcag
     9301 ataaatgaaa acgccagccc tgtgcaggct ggcgtcagag gaatgaagtc tggtgagtga
     9361 gcttcaccgg ttctggtgca gaagctgcag atgacgcagg gtacggacat cctcccgggc
     9421 ctgttcgata cagggcaaca gtgctgctga atccgtttta tcctcatcgt tgtcaaagat
     9481 aattcccgat ccgcagtcga tattgtcctg cagccacgca atcagaatat ccagcgctgt
     9541 ttccgtggtt aatgatttca tgttgttcct ggttaccagt cgaaagtggg taaacccggc
     9601 aggcattcag cgctggcatc cagatgaatg agactgacgc cataacgccg gatgagtgtg
     9661 acgaccagac gacggaacgt ttttgacagc cccaggcgtt taagccgcag aaccgggtat
     9721 gaccacgcat cggtacgtaa cagatatccg ctacctgtaa aatgaatcca ttctgattca
     9781 ccgaagtcac tggtctggtg tgacagcgag tacaaccagg cgttgtccgt ttccgtgata
     9841 tgtgcggtac tgcagcgtat gccggtaagg gtagcaaatg gtggcgtgca ttctgctgag
     9901 gtctggtcag attcatcaac aacacgaagg gagtagccgt tatcgcagac catgttaatc
     9961 agttcagcga gctcaatacc atcgacgtca acgacaatgc gccctatgtt cagtgcctgt
    10021 acgttaacgc tgtcggcttc cagtgtcagg gccagtttca tcatttcgcc tccggatact
    10081 tacccagggt aatgttattt accgttctgt aattgtcgcg ggtcatcagg ccggtcgccc
    10141 ggcgagcccg gaggatatca atactgttta ttaactgaga gcggggacag gtgctgaatc
    10201 ccggctggtc ggtacgcacc agcgcgtatt tttccacgag aaaattaact gcatcacaca
    10261 gtgaaatgcc tgcctcaata tgctgctcaa tcacacgttc atcgacaaac ggtgtgtcat
    10321 tcagtgtgag gccataatgc tggtccagca gtcggatgag cagtgtctgc cagatggtga
    10381 cgggagacgg gcagcgagat acctcccgta catgtgtgtc ggataatgtt ttcatcttaa
    10441 gttctctgta actggtagtg ctaaaggtga gagcttcggg gttatgaggt ggttgcgggt
    10501 gctggtgtcg gataaacagc cagataaacg taaccacagg agccgagggt gtcggcttcg
    10561 caggtcagcc cttttgcata cagcgtgacg gtatgctgat atcggggatt cagttcaccg
    10621 ctggtgagca tgagttccag ttgtttcatc agcagcggaa aggcctggtc cagatggtat
    10681 gcatctgcgt cgctgaagcg gcctctgata ccggcgcggt cggcaaggta atacaggcgg
    10741 ttaccctctt gcaccagacg agcgccaaaa cagggcctca ctgtgcaggg aagcccccac
    10801 caggggcggt cgtgattgtc atcgggatga gttatcccgg agagtttgtc tgacacgata
    10861 aaatcctctc tgaaaagtgg ttaagaatgg tttaacgatg acaggtcagc cggcgcgctg
    10921 accggtttcg gtgaagatgt atgcattgat gagaatggcc tcatccacag cggcgtctga
    10981 ggtcatctgg tcgtactcgt ttcccgggtg acggaacatc agttcagggt gacactcatc
    11041 agccggacat acgggccaaa actgtcctta cggcgttctg caaacacggc aagaacgccg
    11101 ggaatatcct gcacttcacg accagtatac gcttcagcac tgccgtgcca gcgatactta
    11161 ccggtacaga acggaaaaag acgggatgca ggatgctgtt ggtgaatacg catggcttca
    11221 ccacgggtga tgattctcat gatgtgatac ctctgaagac agaagataaa agtgaaaaca
    11281 ggtgtgatgt ggttgtgacg ggttaaagca gaccatgttc ggcaaaggag aaaacctggt
    11341 tgccaccgac tatcagatgg tccggcaccc ggatatccac caggcccagt gcctgtacca
    11401 gacgttcggt gataagccgg tctgccttac tgggtgtgac ttcaccggac gggtgattgt
    11461 gcgccagcac cacggcagcg gcattgtggt acagggcgcg tttaatcact tcccggggat
    11521 gaacttccgt acggttgatg gtgccggtga agagggtttc accggcaatc agctgattct
    11581 ggttgttcag atacagcacc cggaactctt cccgctccag tcccgccatg ttcagaatca
    11641 gccattcccg tgccgcacgg gtggaggtga aggccacgcc gggttcatga agatggcggt
    11701 ccagggtttg cagggcccgc agaatgagac tgcgctcgcc gggcgtcatg tttccgggca
    11761 gaaaggaaag ctgctgcatt gtccctgcct ccattcagtc gatgatgcgc ataatggcgc
    11821 tgcattccgg atgctgcagg gcgtaatccc gcagccggta ataatggacc gtcatggcat
    11881 aacactccgt acgacaggca tgatgactgt acgtcatcag acaggcagca atgccggcgg
    11941 cttccgggct catttcagcg cggttaccgt tcatggcatt gaacagtacc catgtttcgt
    12001 catcatcgtc atccggttcg ggtgccataa atgccccgcc gttgttcagg gtgtacagat
    12061 tccagatacc accgcagtag tcttcacaca gacggtccat ccagccgaag acacggggtt
    12121 ccagggtgac ccactgtgga atgaggccaa aatgctgcgg ccagaagctg atgcgctgtt
    12181 catcagggac gggagtggca accagttgtt gcaggccagt ctccggtgca caggccgaag
    12241 cggtggtgtt ctgagacaat gttttcatgt atgaaatcct tgtaatgtga ataaatgaga
    12301 gggagtaaag gtcgggagct ggcaacatca cgttcatcac tgaccgtgat gacccggtaa
    12361 tatcagggat gaatgatatt cggctgaaat tccggacagc cgtacgcgta accgttccgg
    12421 tcacagacgg atggccagat gccattgcct gtatactcag tctgcacaga ctgatttctc
    12481 cgggacggta aagcagacgg gtacaccagg cgcacaggtc atcatcagaa atggaacgac
    12541 accccggcag cgcctccggg gcgtcgggtg attctggtgc ctgaatcacg ccttcaggga
    12601 catgctgcct cagcgcaggc tctccagcag cgtttctgcc attacccaca atgcgcggtt
    12661 gagcttaata tcggtgtcga tgttgtgaat ggcacgggtg tggatacgtt ttccttttgc
    12721 actgcggccg gaaatcccgc ctttcagcat attctcctgg atggtctgat atgcgctcca
    12781 caggtcttta ccgtaatcct ccctgcgtcg tggtgtcaga atgtcagcgg tggtgactgg
    12841 ctgatgttcg tcaccataac ggtaagtcag tgccgcctgt gccagcgcct ggcgtgccgg
    12901 tggcggcagg accagcgact gcatggcatc acgcttctcc tctatccggt caaacacgcc
    12961 caccacctcg taagcccctt caataacttt ctccaccaca tttccccggt gtggaacacg
    13021 caattctccc agagactgac cgcagaccag tccattggta caaatggcac gaaaatatcc
    13081 gggtaacatc tggtagctgg atgaaccgtc atgagagttg agcagaataa tttcagggac
    13141 atgctgaccg gttatctgtc cggcccgccg cagacgcagc atgtgttttg tgtattcccg
    13201 gcgacccggg tcacgcacac gtgtctggca ggcgaagaat ggctgaaagc cttcccgctg
    13261 caggctttcc agtacggtga tggtgggagt gtacgcatag cgtttactgc gggaagtatg
    13321 ccggtcttca ccgaaaatac tgggaacata gtgcatcagt tcttcgtgtg tcagcggacg
    13381 gtcacggcgt atctggtttg cagcataacc aaaacgactg gctagtcgca taatttgctc
    13441 cttatcggtg gttaagattt actggtgtaa taaatgaaaa agccacgtct cccggagaag
    13501 acgcggcctg atggacgaaa ttaatgaggt ttattgtctg agaagaccgt cactggcgaa
    13561 ccttccggcg tcagtctctg cgttgcggat aacccgacgg aaatgagttc cctggtcatc
    13621 gtcgataaac acattgcggt aagctgttgt gatgacaaca gcctgctcgc gtgagaaagg
    13681 gacttcgggc aataaacgtg acatacaacc agacctgtat ttggttccct gaaaatataa
    13741 gtccctgtca tcatggcaga gccgtattta cttttgatat caataaatta ataacgcgaa
    13801 gatgaaccgc ataacttacg cctttaattt gttttgttta cgtagtccct gaccaggtac
    13861 gcgaattcgt gaacgccagt ttcaccaaac caaatcaggt aaccagggtc gatgttgtaa
    13921 tagtaaccaa cgactttatg cgtttttggg ttatagagtg ttacgatata gtcccagccg
    13981 caggcgtggg ctttacaacc gccaccttct acccaggtcg ttccgttaat gtttacagga
    14041 tacgcaccac catcaggacc atccatatag ccgtcttccc caaacattcc gtttaaccca
    14101 acagggttta atgctttgtc gatagcagct ctgtaggtag cgtcaccttc gtataactcg
    14161 tacgttgttg gacatgcacc aggctggtca gcccgggctt ttgagtcgca tttaagtgtc
    14221 tgatttacag tggcggcgtt tgtgtcagga ctt
//
LOCUS       DAEEGA010000095        14118 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEEGA010000095 DAEEGA010000000
VERSION     DAEEGA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14118
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.095"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(197..2872)
                     /gene="adhE"
                     /locus_tag="KLH46_003257"
                     /gene_synonym="adhC"
     CDS             complement(197..2872)
                     /gene="adhE"
                     /locus_tag="KLH46_003257"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBE0379842.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3349..3996
                     /gene="ychE"
                     /locus_tag="KLH46_003258"
     CDS             3349..3996
                     /gene="ychE"
                     /locus_tag="KLH46_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBE0379843.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            4734..6365
                     /gene="oppA"
                     /locus_tag="KLH46_003259"
     CDS             4734..6365
                     /gene="oppA"
                     /locus_tag="KLH46_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBE0379844.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            6451..7371
                     /gene="oppB"
                     /locus_tag="KLH46_003260"
     CDS             6451..7371
                     /gene="oppB"
                     /locus_tag="KLH46_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBE0379845.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            7386..8294
                     /gene="oppC"
                     /locus_tag="KLH46_003261"
     CDS             7386..8294
                     /gene="oppC"
                     /locus_tag="KLH46_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBE0379846.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            8306..9319
                     /gene="oppD"
                     /locus_tag="KLH46_003262"
     CDS             8306..9319
                     /gene="oppD"
                     /locus_tag="KLH46_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBE0379847.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            9316..10320
                     /gene="oppF"
                     /locus_tag="KLH46_003263"
     CDS             9316..10320
                     /gene="oppF"
                     /locus_tag="KLH46_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBE0379848.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10373..10702)
                     /locus_tag="KLH46_003264"
     CDS             complement(10373..10702)
                     /locus_tag="KLH46_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBE0379849.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(10737..12197)
                     /gene="cls"
                     /locus_tag="KLH46_003265"
     CDS             complement(10737..12197)
                     /gene="cls"
                     /locus_tag="KLH46_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBE0379850.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            12340..12513
                     /locus_tag="KLH46_003266"
     CDS             12340..12513
                     /locus_tag="KLH46_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBE0379851.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(12568..13821)
                     /gene="kch"
                     /locus_tag="KLH46_003267"
     CDS             complement(12568..13821)
                     /gene="kch"
                     /locus_tag="KLH46_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBE0379852.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         3647 a         3285 c         3491 g         3695 t
ORIGIN      
        1 cccagagaag tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt
       61 ccaatgtgca taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact
      121 ggatcggtca actgatcctt aactgatcgg cattacccca gaaggggccg tttatattgc
      181 cagacagcgc tactgattaa gcggattttt tcgctttttt ctcagcttta gccggagcgg
      241 cttctttttt cgctgcagtt tcaccttcta cataatcacg accgtagtag gtatccagca
      301 gaatctgttt cagctcggag atcagcgggt aacgcgggtt agcgccggtg cactggtcat
      361 cgaatgcatc ttcagacagt ttatccacgt tcgccaggaa gtctgcttcc tgaacgccag
      421 cttcacggat agatttcgga atacccagtt cagctttcag cgtttccagc catgccagca
      481 gtttctcgat cttagcagca gtacggtcgc ccggtgcgct cagacccagg tggtcggcaa
      541 tttcagcata acgacggcga gcctgcggac ggtcatactg gctgaatgca gtctgcttgg
      601 tcgggttgtc gttcgcattg tagcgaataa cgttacaaat cagcagggcg tttgccagac
      661 cgtgcggaat atggaactgg gaacccagtt tgtgcgccat tgagtgacat acacccagga
      721 aggcgttcgc aaatgcgata cccgcgatag ttgctgcact gtgaacacgt tcacgcgcta
      781 ccggattttt agacccttcg tggtaggacg ctggcagata ttctttcagc agtttcagtg
      841 cctgcagagc ctgaccatca gagaactcag atgccagtac agaaacataa gcttccatgg
      901 cgtgagttac tgcgtccaga ccaccgaaag cacacaggga cttcggcatg tccataacca
      961 ggttggcgtc gacaatcgcc atatccgggg tcagcgcata gtccgccagc gggtatttct
     1021 gaccagtagc gtcgtcagtt acaaccgcaa acggagtgac ttcagaacct gtaccagaag
     1081 tggtggtgac agcgatcatt ttcgctttca cgcccatttt cgggaacttg tagatacgtt
     1141 tacggatatc cataaagcgc agcgccagct cttcgaagtg agtttccgga tgttcgtaca
     1201 taacccacat gatcttcgcg gcgtccatcg gggaaccacc acccagcgcg ataatcacgt
     1261 ctggtttgaa ggagtttgcc agttctgcac ctttacgaac gatgctcagg gtcgggtctg
     1321 cttctacttc gaagaagact tcagtttcaa cgcctgctgc tttcagtacg gaagtgatct
     1381 gatcagcata accattgttg aacaggaagc ggtcagtcac gatgagcgca cgtttgtggc
     1441 catcagtaat cacttcatcc agcgcgattg gcagggagcc acggcggaag tagatagatt
     1501 tcggaagttt gtgccacaac atgttttcag ctcgcttagc aacggttttc ttgttgatca
     1561 ggtgtttcgg accaacgttt tcagagatgg agttaccacc ccaggaacca caacccagag
     1621 tcagggaagg tgcgagtttg aagttataca ggtcaccgat accaccctga gaagccgggg
     1681 tgttaatcag gatacgagct gttttcattt tctgaccgaa gtaagaaacg cgagccggtt
     1741 ggttatcctg gtcagtgtac aggcaagagg tatgaccgat accgcccata gcaaccagtt
     1801 tctctgcttt ttctaccgcg tcttcgaaat ctttagcgcg gtacattgcc agagtcgggg
     1861 acagtttttc atgtgcgaac ggttcgcttt catcaacaac ggtcacttca ccgatcagaa
     1921 tcttggtgtt ttctggtaca gagaagcctg ccagttcagc aattttatag gctggctgac
     1981 caacgatagc cgcgttcagc gcaccatttt tcaggataac atcctgaaca gctttcagct
     2041 ctttaccctg caacagatag ccgccgtggg ttgcgaaacg ttcacgtact gcgtcataaa
     2101 cagagtcaac aacaacaaca gactgttcag aagcacagat tacgccgttg tcgaaggttt
     2161 tggacatcag tacagatgca actgcacgtt tgatatcagc agtttcatca ataacaactg
     2221 gagtgttacc cgcgcctaca ccgatagctg gtttaccgga gctgtatgcg gctttaacca
     2281 tgcccggacc accagtcgcg aggatcaggt tgatgtctgg gtggtgcatc agtgcgttag
     2341 acagttcaac agaaggttga tcgatccagc cgatcagatc tttcggagca ccggcagcga
     2401 tagcagcctg cagaacgata tcagccgctt tgttggttgc atcttttgca cgcgggtgcg
     2461 gggagaagat aatggcgtta cgggtcttca ggctgatcag cgatttgaag atagcagttg
     2521 aagtcgggtt agtggtcgga acgataccgc aaataatacc gattggttca gcgatagtga
     2581 tggtaccaaa agtgtcgtct tcagacagaa caccacaggt tttttcatct ttataggcgt
     2641 tgtagatata ttcagaagca aagtggtttt tgatcacttt atcttcgacg atacccatgc
     2701 cggattcggc aacggccatt ttcgcgagtg ggattcgagc atctgcagca gccagagcgg
     2761 cggcgcggaa gattttgtct acttgctctt gagtgaaact ggcatattca cgctgggctt
     2821 tttttacacg ctctacgagt gcgttaagtt cagcgacatt agtaacagcc ataatgctct
     2881 cctgataatg ttaaactttt ttagtaaatc atctgctcga atacgagagt atagtcagtg
     2941 cggtgatgat ttgcttaacc gatgaaaatc aaaagcttac tcgcgctcac actcactgtg
     3001 atttactaaa agagtttaaa cattagagtt attatctcta atgcgtcact tccaggtggc
     3061 gtaagcaaga ttactcactt ctgggtactg attacgtgat ccaaatcaaa tttttgcaaa
     3121 gctgacacct ttcagcatcg cttttcgcca ttatagctaa cagttaataa attgtagtat
     3181 gatttggtgg ctacattagc atgttttgca caactagata acaataacga atgatagcaa
     3241 ttttaagtag ttaggaggtg aaaaatgctg tcaaaaggcg tattgtcagc gcgtcttttc
     3301 aaccttattt atggctaaca ttatccggct tttgcttcgg agcgaaccgt gattcagacc
     3361 ctttttgatt ttcccgttta cttcaaattt ttcatcgggt tatttgcgct ggtcaacccg
     3421 gtagggatta ttcccgtctt tatcagcatg accagttatc agacagcggc agcgcgaaac
     3481 aaaactaacc ttacagccaa cctgtctgtg gccattatct tgtggatctc gctttttctc
     3541 ggcgacacga ttctacaact ttttggtata tcaattgatt cgttccgtat cgccgggggc
     3601 atcctggtgg tgacgatagc gatgtcgatg atcagcggca agcttggcga ggataaacag
     3661 aacaagcaag aaaaatcaga aaccgcggta cgtgaaagta ttggtgtggt gccattggcg
     3721 ttgccgttga tggcggggcc aggggcgatc agttctacca tcgtctgggg tacgcgttat
     3781 cacagcatta gctatctgtt tggtttcttt gtggctattg cattgttcgc tttatgttgc
     3841 tggggattgt tccgcatggc accgtggctg gtacgggttt tacgccagac cggcatcaac
     3901 gtgattacgc gtattatggg gctattgctg atggcattgg ggattgaatt tatcgttact
     3961 ggtattaagg ggattttccc cggcctgctt aattaattcc tttcaaatga aacggagctg
     4021 ccatgctccg tttacttcgt cattattttt actttgttcc cgcgcagtta tcaaaagcaa
     4081 aaggaatagg taaaaatatt cttctcaaat tacagttagt tataaggatt tccttaactg
     4141 tttctcctca ccatcatgtt attttcgcca catcataatc ctgggcttgc tgaagaataa
     4201 ttgaaatgat attagtaatt ccactgcctt tggtagagga aagtgctaaa taataatcaa
     4261 ttgttaaatt attgtgcatt tcactactgg aactgtaatc agaaaagata gacatgctta
     4321 gccaatctct atttgattga attgaaagat gtttgttaag gcatggatgc aagctataga
     4381 ttctgatacg gtcaataaaa gagaatcgct taacaatttt gcaaaatgta ttggcgagta
     4441 agaaccgcat ttggtacttt ccgggcaacc gccagacgat tctttattgg taatgagaat
     4501 aattaacaat taaagagcgt cgcgaaagaa taatgtgtct cgacagggga gacacagtac
     4561 gaatcgacat aaggtgatcg tctgaatcac cagaataaat aaagtcggtg atagtaatac
     4621 gtaacgataa agtaacctga cagcagaaag tctccgagct tgtgcagggt cccaatccgg
     4681 gattacacat gctggttaat accagtaatt ataatgaggg agtccaaaaa acaatgacca
     4741 acatcaccaa gagaagttta gtagcagctg gcgttctggc tgcgctaatg gcagggaatg
     4801 tcgcgctggc agctgatgta cccgcaggcg tcacactggc ggaaaaacaa acactggtac
     4861 gtaacaatgg ttcagaagtt cagtcattag atccgcacaa aattgaaggt gttccggagt
     4921 ctaatatcag ccgagacctg tttgaaggct tactggtcag cgatcttgac ggtcatccag
     4981 cacctggcgt cgctgaatcc tgggataata aagacgcgaa agtctggacc ttccatttgc
     5041 gtaaagatgc gaaatggtct gatggcacgc cagtcacagc acaagacttt gtgtatagct
     5101 ggcaacgttc tgttgatccg aacactgctt ctccgtatgc cagttatctg caatatgggc
     5161 atatcgccgg tattgatgaa attcttgaag ggaaaaaacc gattaccgat ctcggcgtga
     5221 aagctattga tgatcacaca ttagaagtca ccttaagtga acccgttccg tacttctata
     5281 aattacttgt tcacccatca acttcaccgg tgccaaaagc cgctatcgaa aaattcggcg
     5341 aaaaatggac ccagcctggt aatatcgtca ccaacggtgc ctatacctta aaagattggg
     5401 tcgtaaacga acgaatcgtt cttgaacgca gcccgaccta ctggaacaac gcgaaaaccg
     5461 ttattaacca ggtaacctat ttgcctattg cttctgaagt taccgatgtc aaccgctacc
     5521 gtagtggtga aatcgacatg acgtataaca acatgccgat cgaattgttc cagaagctga
     5581 aaaaagagat cccggacgaa gttcacgttg atccatacct gtgcacttac tattacgaaa
     5641 ttaacaacca gaaaccgcca ttcaacgatg tgcgtgtgcg taccgcactg aaactaggta
     5701 tggaccgcga tatcattgtt aataaagtga aagcgcaggg cgacatgccc gcctatggtt
     5761 atactccacc gtatactgat ggcgcaaaat tgactcagcc agaatggttt ggctggagcc
     5821 aggaaaaacg taacgaagaa gcgaaaaaac tgctggctga agcgggttat accgcagaca
     5881 aaccgttgac catcaacctg cggtataaca cctccgatct gcataaaaag ctggcgattg
     5941 ctgcctcttc attgtggaag aaaaacattg gggtaaacgt caaactggtt aaccaggagt
     6001 ggaaaacgtt cctcgacacc cgtcaccagg gcacttttga tgtggcccgt gcaggctggt
     6061 gtgcagacta caacgaacca acttccttcc tgaacaccat gctgtcgaac agctcgatga
     6121 ataccgcgca ttataagagc ccggcctttg acagcattat ggcggaaacg ctgaaagtga
     6181 ctgacgaggc gcagcgcaca gctctgtaca ctaaagcaga acaacagctg gataaggatt
     6241 cggccattgt tcctgtttat tactacgtga atgcgcgtct ggtgaaaccg tgggttggtg
     6301 gctataccgg caaagatccg ctggataata cctatacccg gaatatgtac attgtgaagc
     6361 actaatggca atacgtgggg caggagtgtc ctgctccacg gtgtctgatt tttatcgcat
     6421 tacagaaggc acaggccaga aggtagggca atgttaaaat ttattctacg tcgttgtctg
     6481 gaagcgattc cgacgctatt tattcttatt actatttcgt tctttatgat gcgcctcgcg
     6541 ccgggaagcc cttttaccgg cgaacgtact ttaccgccag aagtgatggc caatatcgaa
     6601 gcgaaatatc atcttaatga tccgatcatg acacagtatt tcagctacct gaaacaactg
     6661 gcgcatggcg atttcggtcc atcgtttaaa tataaagatt attcggtcaa tgacctggtg
     6721 gcatccagtt ttcccgtttc tgccaaactg ggagccgccg catttttcct tgcggtaata
     6781 ctgggtgtta gtgctggcgt tattgccgca ttaaaacaaa acaccaaatg ggactatacc
     6841 gtgatggggc tggcaatgac cggggttgtt atccccagtt ttgtggttgc gccattatta
     6901 gtcatgatat ttgcgatcat tttgcattgg ctgccgggcg gtggctggaa tggtggggcg
     6961 cttaaattca tgatattgcc gatggtggcg ttgtcactcg cctatatcgc cagtattgcg
     7021 cgtattaccc gtggctctat gattgaagta ttacactcca actttattcg tactgcccgg
     7081 gcgaaagggt tacctatgcg gcggatcatt ttacgccacg cattaaaacc tgctctgtta
     7141 cccgtgctct cctatatggg ccccgcattt gtcggcatta ttaccggttc tatggtcatc
     7201 gaaaccattt atggtttgcc ggggattggg caactgttcg ttaatggcgc attgaaccgt
     7261 gactattcct tagtgttaag cctgaccatc ctggttggtg cgttaaccat tttgtttaat
     7321 gccattgtcg atgtgctata tgcggttatc gacccgaaaa tccgttactg atactggagc
     7381 tcgcgatgat gttaagtaag aaaaacagcg agacgctgga aaatttcagt gaaaagctgg
     7441 aggtcgaagg gcgcagcttg tggcaggacg cacgtcgacg ttttatgcat aaccgtgcgg
     7501 cggttgccag tctgatagtg ctggtgctga tcgcgttatt tgtaatcctg gcaccgatgc
     7561 tttcccagtt tgcctatgac gatactgact gggcgatgat gtccagcgcc ccggatatgg
     7621 agtccggtca ctactttggt actgactcat ccggtcgcga cctgcttgtg cgcgttgcga
     7681 ttggcgggcg tatctcactc atggtcggtg ttgctgcggc actggtggca gtggtcgtgg
     7741 ggacacttta tggttcgctt tccggttatc tgggcggtaa agtggattcg gtaatgatgc
     7801 gtctgctgga aatcctcaac tccttcccat tcatgttctt cgtcattttg ctggtgacct
     7861 ttttcggcca aaacatcctg ctgattttcg tggcgattgg catggtttcc tggctggata
     7921 tggctcgtat tgtgcgtggg caaaccctga gtctgaagcg caaagagttt attgaggcgg
     7981 cacaagttgg cggtgtatcg acgccgggca ttgttattcg ccacattgtg ccgaacgtac
     8041 tcggtgtggt ggtggtctac gcatcgctac tggtgcccag catgatcctc tttgaatctt
     8101 tccttagctt cctggggttg ggtacgcaag agccgttaag cagctggggg gcattgctga
     8161 gtgatggcgc gaactcgatg gaagtctctc catggttact gttgttccca gcgggattcc
     8221 ttgtggtgac actgttttgt ttcaacttta tcggcgatgg cttgcgtgat gccctcgacc
     8281 cgaaagatcg ttaaggagtg cagccatgag cgtaattgaa actgcaactg tgccgctcgc
     8341 acaacaacag gctgacgcac tgctgaacgt gaaagatttg cgtgtcacct ttagtacccc
     8401 ggacggcgac gtcacggcgg tcaatgattt aaatttttcc ctacgtgccg gagaaacgct
     8461 gggcattgta ggtgagtctg gttcgggtaa atcgcaaact gcatttgcgt tgatgggcct
     8521 gttggctgcc aacggacgca ttggcggatc ggcaaccttc aatgggcgtg aaatcctcaa
     8581 tttgccagag cgtgaactca ataaactgcg cgctgaacaa atctcaatga tttttcagga
     8641 cccaatgact tcgttgaatc cctatatgcg cgtcggtgag cagttgatgg aagtgctgat
     8701 gctgcataag aacatgagca aagctgaggc gtttgaagag tcggtgcgga tgctcgatgc
     8761 ggtaaaaatg ccggaagcac gtaaacgcat gaaaatgtac ccgcacgaat tttctggcgg
     8821 catgcgtcag cgagtcatga ttgcgatggc attgctatgt cgacctaagt tgctgattgc
     8881 ggatgaaccc actacagcgc tggacgtcac cgtacaggcg cagatcatga cgctattgaa
     8941 tgaactgaag cgggaattta atactgccat cattatgatc acccacgatc ttggcgtggt
     9001 ggcggggatc tgtgacaaag tgctggtaat gtacgccggg cgcacgatgg aatatggcaa
     9061 tgctcgcgat gtcttttatc aacccgttca tccttattct atcggtttgc tcaacgcggt
     9121 gccgcgtctt gatgcggaag gtgaaacaat gttgaccatc ccgggtaatc cgccaaactt
     9181 gctgcgatta ccgaaaggtt gcccgttcca gccacgttgt ccacatgcga tggaaatttg
     9241 tagtagcgca ccgccgctgg aagagtttac gcctggccgt ctgcgtgctt gctttaaacc
     9301 ggtggaggaa ctgttatgaa tgctgtaact gaaggaagaa aagtcctcct tgaaatcgcc
     9361 gatcttaaag tgcactttga aatcaaagat ggcaaacagt ggttctggca accgccgaaa
     9421 acgctcaaag ccgtcgatgg tgtcactctt cgcctgtatg aaggggaaac attaggtgtg
     9481 gtaggggaat cgggatgcgg taagtccacc tttgctcgcg ccatcatcgg tttggtcaag
     9541 gcgaccgacg gtcatgttgc ctggttaggt aaagagttgc tgggcatgaa gcccgatgaa
     9601 tggcgtgccg ttcgcagtga tattcagatg attttccagg atccgttggc atcgctaaac
     9661 ccgcgtatga ccatcggcga gatcatcgct gaaccactgc gtacttatca tccgaaaatg
     9721 tcacgccagg aagttcgcga gcgcgtgaag gcgatgatgc tgaaagtcgg gttattgcct
     9781 aacctgatta accgctatcc gcatgagttc tccggtgggc agtgccagcg tatcgggatt
     9841 gctcgtgctc ttattcttga accgaagctg attatctgcg atgagccggt ttcggcgctg
     9901 gacgtgtcaa ttcaggcgca ggtggttaac ctgctccagc agctgcaacg tgagatggga
     9961 ttgtcattaa tttttattgc tcatgacttg gccgtggtaa aacacatttc cgatcgtgtg
    10021 ttggtgatgt atctcggcca tgcagtagaa ctggggacct atgatgaggt ctaccacaat
    10081 ccactacatc cttacaccag ggcattgatg tcggcagtcc ccatacctga tccggatctg
    10141 gagaagaaca aaaccatcca gttactggaa ggggaattac cgtcgccgat caacccgcct
    10201 tccggttgtg ttttccgtac ccgttgcccg attgccggtc cggagtgcgc caaaacacgt
    10261 cctgttctgg aggggagttt cagacacgcc gtttcttgcc tgaaagtcga tccgctttaa
    10321 aaacaataag ggctgacagt tgtcagccct ttttcacgct aaaagcgatt atttattccc
    10381 gccagatgat atggcaaagt ttgtgatctt tttcgcggca taacagaatg cgggcgaaaa
    10441 catcgttgat ttcaccatct tcactgtccg ccagaccaat caccacttcg gcaaaaaagt
    10501 ccgggttcaa atcgaaatca acatgttcct gccagtcttc cgccggatcg aataactccg
    10561 cgccgccgcg ctcttcaaac tgaagattga acagcagaac atcagctgga tcgagattgt
    10621 cggcagccag ttcgagaaaa atatcgtagg cctgctcaag cgtttcatct tcagtcaggc
    10681 gattgtttag atccatatcc atagtcacta cctgtttaac ctctgttggc gacgttttac
    10741 agcaacgggc tgaagaagta aaacagtcgc tcggcgacac gttgccataa tggacgtttt
    10801 agccataaac gggcatcgag cagacgtgaa cgcgaaatat aatcgtcctg aacggcggca
    10861 aggtctgcac caaaaccttt atcgtcgatt gccaaggtaa tctcgaaatt cagccacaga
    10921 ctacgcatat caaggttaac ggtgccaacc agacttagtt cgccatcgac cagcacgctc
    10981 ttggtatgca gtaacccgcc ttcaaactga taaattttaa ccccagcagc aagcagttcc
    11041 gtaaagaatg cgcgactggc ccagccgacc agcatcgagt cattttttcg cggaaggata
    11101 atactgacat ccaccccgcg ctgcgccgcc gtgcaaatcg catgaagtaa atcatcgctt
    11161 ggcacaaagt agggcgtggt catgatcaaa tattcatgcg ccgaataagc cgcagtcaat
    11221 aatgcctggt gaatgagatc ttccggaaag ccggggccag aagcaattgt gtgaatggtg
    11281 tgaccgctgg cctgttcaaa cggcataata ttgacatctg gtggtggcgg cagaatacgt
    11341 tttccggttt caatctccca gtcgcaggaa taaataatcc ccatcgcggt ggcgatgggg
    11401 ccttccatac gcgccatcag atcaatccat tgccctacgc ccgcatcttg tttgaagtag
    11461 cgaggatcga ccatattcat gctgccggtg tacgcgatgt aattatcgat catgatcatc
    11521 ttgcgatgtt ggcgcaggtc catacggcgt aaaaacacac gcatcagatt gacctttaag
    11581 gcttcgacca cttcaatacc ggcattacgc attagctcgg gccacgggct gcggaaaaaa
    11641 gccacactcc cggcggagtc gagcatcaat cggcaatgaa taccacgccg tgcagccgcc
    11701 attaatgatt cagccacctg gtccgccatg ccgccgggct gccagatata aaacaccatc
    11761 tcaatattat ggcgcgcgag ctggatgtcg cggattaacg cctgcatcac atcatctgac
    11821 tcggtcatca gctgtagctg attccctttg accccagcga tcccctgacg acgctcgcaa
    11881 agcttgaata atggcgcagc gacactgcta ttttcttcgg cgaagatatg cttacaggct
    11941 ttaaggtcgt taagccattt tgcggtggaa ggccacatcg ctctggcgcg ctcagcgcgg
    12001 cgtttgccta aatggagctc gccaacggca agataggcaa taattccgac taacggcaga
    12061 atgtaaataa tcaacagcca ggccatcgcg gagggaactg cgcgtcgttt cattagaatg
    12121 cgtaaagtta cgcctgcaat gagcaaccag tatcccagaa tggccaacca actcaccaac
    12181 gtataaacgg ttgtcataga ttagaaatcc ttttgaaagc gcattgttat gagtttacgc
    12241 atatggattc atctggcaaa taaaaacgcg ggaaaagcac tggtcagcgt cgggggcggg
    12301 ggtataatga ccattctgtt attgcataga gtagttaaca tgaagcggag tagaacggaa
    12361 gtggggcgct ggcgcatgca gcgtcaggct agccgacgta aatcgcgttg gcttgagggg
    12421 caatcgcgcc gaaatatgcg tatccacagc atcaggaagt gcattctaaa caaacagcgt
    12481 aactcgttat tgtttgcgat ctacaatatc taaatgtact gggcaccatt gcggtgccca
    12541 ataagtttac tcttccgtct ttaaactcta tttttgcgcc gattctttac tgtcagcttt
    12601 cgtttcttgt tcatcgttat cgctgaaaat accatgacct gagttcaaca acattgaaac
    12661 gagcatatca ttattaatct cttcaccatt taaaactcgc gccagaattt cgctgccaaa
    12721 cagttgcggt gagagaatga tatccggatg taccatctta atcttattca ggtttttgct
    12781 atcactgacg gcgagaactg ttttgacatc actgctcata tcttttgccg agagtacaac
    12841 aaacgcgtta tctgcatcgt tatcactcag cgccagaatg gcccggcaac gatcgattcc
    12901 cgctttcttt aatactgaac tgtcattact gtcaccgggg ataacatcag cgttatcgcc
    12961 taagcgttgc tcaagttgct tgatatcatc ttcaggcaag ttgctgataa ccgttacgtt
    13021 ttgtccgcgt tgattcagtt gcaggatcgt attgatggcg agaatcgaat gtccgcaaac
    13081 aataaaatga tctttacgat gcattgtatg attgtttcct tttaaaagtt tgttgaatcc
    13141 cccgcggata agcggaccaa aaattgaggt catagatgtg gcaaaaacgg taatgccgga
    13201 aataatgacc gaaatagtga acaatcgtgc tgattcagaa acagggacaa tatctccata
    13261 gccgacggtt gacatggttt ctatcgaaaa atagaacgcg gtcatcaaac tttctattcg
    13321 cggattaaaa ccttcgctta aataaagcgc accgtaggtt gagtaaaaca gtaatgtcgt
    13381 gaaactaata aatgcaaaaa ttgtcccggc tgcggcgcta ctgtgggaga agtctttgcg
    13441 cagtatcagc aaaaacacca gcgtaaaaat gcaaaatcca atactaaatt tcagccaggg
    13501 ataaaaatgc agggtgtaaa ttagcgctat caacaacaaa atgatactga ttgcccatgc
    13561 gagctttgcc cggaacagta agccaatgga gttcaggaca agaaagacgc cgagcataaa
    13621 tagtggggcg tttgccagca aggagagatc caactctgaa aatgctttga tatggaaaat
    13681 atcaagcaaa ttgaccgaca taccatagat tgttttaaaa ataagcaatc catttaaaaa
    13741 gacggccagc gcgagaatgt cgtgccgtag ggtcacccat aaatttgttg ctgtttgttt
    13801 gaatgtagcc cagtgactca ctgatacctc tgtaaccaca attttgttta attatcttag
    13861 cgtgcacatt ttctgaacat acatgcagcg cgtgaatgtg ttaaacaata gtagatgact
    13921 ttatccgcag taatcaaagg ttttgagtga gcaactctgc gtcaggaaag ggcttttttc
    13981 ctgaacatgc tcacatcata taaagaaata cagataaagt tattatctgc ttgtggtagt
    14041 gaatgcactg accggctata aggaaaggcc aaacaagaac acggttgcaa aaaccgtgcc
    14101 cttaaatatt ggatctct
//
LOCUS       DAEEGA010000096        13963 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEEGA010000096 DAEEGA010000000
VERSION     DAEEGA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13963
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.096"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="KLH46_003268"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="KLH46_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBE0379853.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="KLH46_003269"
     CDS             2219..2767
                     /locus_tag="KLH46_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBE0379854.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="KLH46_003270"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="KLH46_003270"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBE0379855.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="KLH46_003271"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="KLH46_003271"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBE0379856.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLSIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="KLH46_003272"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="KLH46_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBE0379857.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="KLH46_003273"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="KLH46_003273"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBE0379858.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="KLH46_003274"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="KLH46_003274"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBE0379859.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="KLH46_003275"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="KLH46_003275"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBE0379860.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="KLH46_003276"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="KLH46_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBE0379861.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="KLH46_003277"
     CDS             complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="KLH46_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBE0379862.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3552 a         3562 c         3525 g         3324 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gctaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgctgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcccggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgtcg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgc
//
LOCUS       DAEEGA010000097        13799 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEEGA010000097 DAEEGA010000000
VERSION     DAEEGA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13799
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.097"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..817)
                     /gene="wcaD"
                     /locus_tag="KLH46_003278"
     CDS             complement(<1..817)
                     /gene="wcaD"
                     /locus_tag="KLH46_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBE0379863.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(792..2009)
                     /gene="wcaC"
                     /locus_tag="KLH46_003279"
     CDS             complement(792..2009)
                     /gene="wcaC"
                     /locus_tag="KLH46_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBE0379864.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREILALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2006..2494)
                     /gene="wcaB"
                     /locus_tag="KLH46_003280"
     CDS             complement(2006..2494)
                     /gene="wcaB"
                     /locus_tag="KLH46_003280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBE0379865.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2497..3336)
                     /gene="wcaA"
                     /locus_tag="KLH46_003281"
     CDS             complement(2497..3336)
                     /gene="wcaA"
                     /locus_tag="KLH46_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HBE0379866.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3429..5591)
                     /gene="wzc"
                     /locus_tag="KLH46_003282"
     CDS             complement(3429..5591)
                     /gene="wzc"
                     /locus_tag="KLH46_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBE0379867.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5594..6037)
                     /gene="wzb"
                     /locus_tag="KLH46_003283"
     CDS             complement(5594..6037)
                     /gene="wzb"
                     /locus_tag="KLH46_003283"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBE0379868.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6043..7182)
                     /gene="wza"
                     /locus_tag="KLH46_003284"
     CDS             complement(6043..7182)
                     /gene="wza"
                     /locus_tag="KLH46_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBE0379869.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7491..7640
                     /locus_tag="KLH46_003285"
     CDS             7491..7640
                     /locus_tag="KLH46_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379870.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7841..9424
                     /locus_tag="KLH46_003286"
     CDS             7841..9424
                     /locus_tag="KLH46_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBE0379871.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9876..11729)
                     /gene="asmA"
                     /locus_tag="KLH46_003287"
     CDS             complement(9876..11729)
                     /gene="asmA"
                     /locus_tag="KLH46_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBE0379872.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11751..12332)
                     /gene="dcd"
                     /locus_tag="KLH46_003288"
     CDS             complement(11751..12332)
                     /gene="dcd"
                     /locus_tag="KLH46_003288"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBE0379873.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12424..13065)
                     /gene="udk"
                     /locus_tag="KLH46_003289"
     CDS             complement(12424..13065)
                     /gene="udk"
                     /locus_tag="KLH46_003289"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBE0379874.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13383..>13799
                     /locus_tag="KLH46_003290"
     CDS             13383..>13799
                     /locus_tag="KLH46_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HBE0379875.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLG"
BASE COUNT         3193 a         3640 c         3494 g         3472 t
ORIGIN      
        1 tacctaaatc gcccagacgg gtggaaatat aaggaaacgc gattaccaca ccaacccgga
       61 atacagccag agaaatcaat gctaacggta attttttctt aatcgcctct ttattcagat
      121 attgaaatgc ccactccagc aaataaaaca ggataaaggt cataacccct gaaaacgatc
      181 cggataatat tatccctgcg agaatcaggg catctgtttt aggcgttttg ataccaaact
      241 gtttgatgct gagccaaatt gagattaatg ccagagcgaa aaatgccggt tcgaaataaa
      301 gtgctgtcgt gcgcttgccg ccgaatttaa tgaaattcag cacatagctg ttgctgtaaa
      361 tcagatattt cgaaatcgac tccataatac tgctgccacc ggtgaggata atttgcgcca
      421 tctccaccgc cgccagcgcc accaccagtc cgaccaccag ataaaagaaa cgtaatatct
      481 tacgatggtt gtgtggcgaa attgttttaa aacgaatact ccacaccatg ccaataatga
      541 tcacaatata gacaaacagc atagttgaag tgacgtattt accggcatcc aacgactgac
      601 caaacaggta gttaaacgcc gtgagtcccg cgccaatccc taaggcaatc atcaattttt
      661 taatgctgat gcgttctaaa aacagcagta acaagacagg taaaaaagtg acgatggtga
      721 tggggaaact ttcgccaagc tgggcgattt tgacgttaac cagcaggtag atcagtggca
      781 gcagcaggta gctacagatt ctgatagaag ttgacatact cctccagcat ctgttgtcca
      841 ctgtaggcgg cgcgactgcg ttggctgaac tcagccagcg tggtaccaaa tatcgcctgc
      901 gcgatttccg gtttgcttaa ctgtaccagt tgcagcacct cttcttcact gacggtttta
      961 ccaccggatt tttgcagcac ttcccgcgcc gcatcgctat gggtggcaat caccggcacg
     1021 ccaatcgata gcgcctcaca caaaatcagc gggtagttat cgacacgaga actgaacacc
     1081 agcgcatcca tctgattgag cgcgctcatc aacttgcgct tgtcagtttc aaagccgtga
     1141 ttaaccacgt tgccagcggt gaacggcgag aacttaccaa aggtatgcag ttcgattttg
     1201 tcgcccagcg ccatcatctc acgcaccagt tgctggttag ttttgccgtc gtaacgcagg
     1261 tcatgcgcca ccactgcgat tttcggcttg ccctgagttt cgcgcaccgg aggcagatct
     1321 gccagaatcg cttcggttgc catatcaatg ccattattga taatccggca acgccctggc
     1381 ccgtacagac tattgaaagc gtcagccaca tgctggctgg gggaaataaa ctgacagccc
     1441 agcgccagta tctcgcggaa taactggcgt ttgcccgcca ccagctggtg cgcgcgatca
     1501 atcttcaccg gcggatagtt atttaaggtc gggcatttct ggcagcccgt tttccagcct
     1561 tcgcaaccgt cggtaaaggc gcagcgtccg gttacactcc agtggtcgtg cagcgtccag
     1621 accagggtga catccggttt atggtttttc actttttcgc aaaagcgcac cacgctgtta
     1681 agattcaacc agtagctgtg cagcacatga aaatgcagga ccaccggacc cggtgtgcga
     1741 gtaatggtgc gatataactc attgaaattg ccgaacagat cgcgattaaa cagacgaaac
     1801 aacgcaatgt tcgccatcgc ggtcatccgc ggcgtatgtt tgatgacctg cggatagttc
     1861 tgatggctga cgctctcttt gccgcctttg ccgtagccgt agacaaaatg tgacgccagc
     1921 ccctgttgca gcgcacgctg gtggagatct aacgccacac ctgctgcccc gccttccgcc
     1981 agtcgcacat taaattgcag aatattcatt taatcacctt cactcgcgct ttttctccca
     2041 ccaccagcgc gttgtccggg acagagtcga gcaccacgct gcccgcaccc acggtgacgt
     2101 tgttaccaag cgtgatatca ccaagaataa tgacgttggc accgagttcg acgccgttgc
     2161 caatgtgtgg acatgccatg ttatcggcac cccgattgcc gatagtgacg ccgtggcgaa
     2221 tggtgaaatc atcccccgcc accacgtttt tattgatcac tacggcgtaa ccgtgatgga
     2281 tagtaaagcg gcggccaatg gtcgcggcgg cctggatttc ataaccgaaa aagcattcgg
     2341 tgataatgcg atacagcacc agcagcgggg ccgcccacag attgttgagg acgttctttt
     2401 tgcgccacac cgaacaaaaa tgagcgacgc gataggcaag taccatgcag cacgggcgta
     2461 aactccagct gttggcgcgc agatcttcca gcatctttag cgcccccgaa taccatcagc
     2521 cagacgtttg ccgttacgca ccgacagcag cgtcaacaag gtgcgccagg tcatacgttt
     2581 attgcggatc tggtagaggg taaacagctg atatttttta ctggcgcggt cgaatttgtc
     2641 tttgtgcttg cggtaaaagt ggaagtatcc ggagaatttt ttcggtgacg aggtgatctg
     2701 catttcgccg tgattgatat gcaggatctg cgtcgcctct tccactttcc acggttcgcc
     2761 gtactccacc accatccgca ggaatatgtc gtaatcctgt gctgctttca gttcggtatc
     2821 gaacaaacac tctttgaaac gccatgccca ggtaaagacc tgattgccaa tgatattacg
     2881 tttatagaac aggcggcgtg agtacggcga tttgggatac agtggtaagc tcgccggttg
     2941 ggaataaact tctccctggc aaacgtagtc gttagcgtac aaaaaggcgt gtgtgaccag
     3001 ttgctgttta tgggcgagga agacgctcag gcggttgggc gtccattcat catcatcatc
     3061 gatcccggtg atgtattccc cttgcgccag cataatcgcc tggttacgca ccgcgcacgc
     3121 cccgctgtta atgtcgttgt ggatgtaagt gatacgcgga tcgttgaggg cagtgacgta
     3181 ctgttgcagt tgttcccagg aggtggagca atcatccacg atgatcatct cccagttgct
     3241 gtagtcctgg cgcaggaccg attttatcgc ccgaatcgcc agttgttggc ggttccaggt
     3301 tggcatatag attgagatca gcggattgtc tttcataggt tgttctcccg gcttatgagc
     3361 tggtttgctt gccggatgcg gcgtgagcgc cttatccggc ctacggggcg gtgcgaatgc
     3421 aggcgtcgtt atttcgcatc cgacttatat tcgtattcgt aatagccata atcctgatac
     3481 gcgctggcgc ggcggaagat ggagttcaga atcacccctt tcaccggaat accgttttgc
     3541 tcaaagcggc tcagactggt ttccacttct ttcaatgtgt tgaccgcata acgcgccacc
     3601 attaacgtgg ttccgacatg acgaccaaca attgccgcat cggtcactgc cagaatcggc
     3661 ggcgtatcaa tcaacaccag gtcatagttt ttactcgccc agttcaccag ttcggcaaag
     3721 cgttcgctca ttaacagttc agaaggattt ggcggcacct gaccgcgcgg gatcaggtca
     3781 aatttggcaa tagaggtcgg tttagcagcg gtagtaatat cgccctgacc aatcagaatc
     3841 tccgacagcc cattgacatt attggtgccc aacagctcgt gggtgtagcc tttgcgcata
     3901 tcgcagtcga tcaacaacac gcgtttattg gtctggctga tcaccgccgc caggttggcg
     3961 cagacaaagg ttttaccgat tgacgggcta accccggtca tcatcaacac attgttctgc
     4021 gcctgcatca tcgcgaagtg cagactggta cgcagactac ggatggcttc aatcgccaga
     4081 tcggttggat tccccactgc cagtagctgg ctctgtttat agcgtttaac ccctttgatg
     4141 gttttgacgc tatcgcgcgc tttctgccac tccgacagcg ggatgctggc atagacgctg
     4201 attccgtgtt cttccagcac ttgcgggctt tcgatgccgc gattaaacaa cgagcgcagc
     4261 agcacaccca cgatagagag catcaggcca aggataatcg cgccgaggat aatcagccct
     4321 ttcttcggtt ttagcacgcc aggctgagtg attgccgggt caataatgcg cacatcgccg
     4381 acggtgctgg cttcggtgat tttcagctcc tgctctttat tcagcagttg catatagacc
     4441 tgctgaccag actcgacatc gcgggtcaga cggacaattt cctgctggtt tttcggcatc
     4501 gccgttacgc gaccgttaag tttggctttc tcttcttcca gcgcctgacg tttctccagc
     4561 agcgtgcggt acgccgggtg aactttggtg tacagcttgg agatttccgc ctctttaaag
     4621 gtcagttcgt tcaactgcgc gtcgatgttc accatcgaat cgagcaccgc tttcgcttca
     4681 agcggcagat caacagaatc tttatcctga cggaaggcat tcagtttgtt ttcggcaaca
     4741 tcgaggcggc tacgtacttc cggtaactgt tgcgcgagga aagcgaggct tttcgacgct
     4801 tccgccgatt tgcgctcaat attttgttcc tgatagttac gagcgatgct gttaagaatg
     4861 tcgcggatct gttcgcgatc ttcaccggta taagtcaggc tcagtacgcc tgcgtctttg
     4921 ccgttctccg ttaccgtcag gctgttttgc agttggttga tcatccccaa cgtggagtat
     4981 ttggtgacgg taaactcact gcccgggcgg gcgtgaatgg cttcaaccat cagcgtgacg
     5041 ccttcttttt tcagcatctg gcccgcttgc ccacgggcgc taaagccgcc atcactgctc
     5101 agggtgtagt ttttgtcatc cagcacatta agcgtaaaca cctgatccgc catctctttc
     5161 gggcggttaa aggtagtcac tttcaccgtc tcgttctgac gccccatcag gcgatcccag
     5221 cccgccccga aaatcgggaa cgtgtttttg ctcaccgcaa tatcgaggtc gagatcgtcc
     5281 accgttttac caagcaccag acgcgaacga atcaactgga tctcggcgtc cgatgcaggc
     5341 ggtttgttgg ctaatgccga gccgatatcc tgcaccagcg aattgccgct gttttgctcg
     5401 atttgtacca gtgcgtcggc actataaatc ggcgtggcga agaaagtgta aaccacggca
     5461 cagagggcaa atacgacggt gatgccaatc acccaccagc gcgcttcaat gacggtgccg
     5521 accaggcgac caatatcgat ttcatcactg cccgttaccg gagcggcatg ttgttttact
     5581 ttttctgtca ttcttatacc tgctctgcgt tcaatgcctg cgcccactgg cgggcagacc
     5641 gttcaagtaa tgtgtacacc gctgcaaacg tttcccggct tttgcgatac ggatcgggga
     5701 tttcacattc gttatcccag tgaccaaaca gcatcacttt gccgcgcatc tccggtgcca
     5761 tctcgcataa gcgttcgata tggcgcttat ccatggtcaa aatcaggtca tagttgcgac
     5821 acaggcggcg gctgatttgt cgtgcacagt ggccttccag agacagttga tgttctgcgg
     5881 cgacgctgat agcggtagga tcagcgccct taccgaccag cgcgccgagt ccagcggact
     5941 ccactttcag ttccgggtga taacgttgca gtaagcgttc cgccatcggg gaacggcaaa
     6001 tattgccgac acagacaact aagatgttgt taaacatgac gattaccagt tatgaatgtc
     6061 gctggctgta tccgtcatgt aacggacacc gctaatagtt ggcagcaact gattgatcag
     6121 acggttccag cgggaaaccg gggcggtggt gacatacacc acgtcataag gttgcaggcg
     6181 gaattctgtc gccatcacca gcgacgtcgc atcggacata tccagctggt agatattggc
     6241 aatcttgccg ttacgcccgc cctcgccttt cagcggacga atgacaaaga tgccgctggc
     6301 gttggaggtg gtcatgtcga taccttcggc attggccaga gcttcagtca gggtcatgcc
     6361 gctaaagtcc attttcaggg tgctctgttt cttcacttca cccatcacaa atactttcag
     6421 atcatcatta cgcggcacgt agagaatatc gccgggataa agcagtcggt tctgattgag
     6481 gtcgccgttt tgcatcagcg cctgtaaaga aatgcgttct tcacgaccat tgtgtgttag
     6541 caccacgttg cgccagtcag cggtgtcggt caggccacct gcggcgttga tggcgtcgag
     6601 aatggtcagt ggtacgttgg tgatcgcctg ttgaccggat ttattcacct gacctgagat
     6661 ataggccttt tgtgagcgga aggcggcgat attaacgtcc acctgcgggt cagcgatgta
     6721 cgtcgctaag cgcccggtaa tatcactgcg gatttcagcg agcgttttcc cgactacgtg
     6781 aaccttgccg atatacgggt aaaacatagt gccgtcaggc tgtacccagt tgccggtgtc
     6841 gctggagctg cggtactgac cagctggcgt ggtgagttcc gggtgatccc agacggtgac
     6901 attaagaacg tcccccggcc cgacgcgata ctggtaattc gcgatctcgc tttccagcgt
     6961 catattgggg cgcgctacat tcgggcgtgg gcgtaattgg tcaatcaggc gcggggtcag
     7021 cggataaaca ttcaccattt tgtcgagatc gaaatcagcg tcctgctgtt tgatgacgtc
     7081 tttgcccatc gtcgacatat tgctgcccgg aagtactgtg caaccgctta tcaaggttac
     7141 tgacaccaat aatggcatca atttcatttt ggatttcatc attgtttatt tatcactttg
     7201 gcagagtaat tatcctgtgc actattaata gcaatgtcgc catgcacatt taccttgcag
     7261 ttaattgaat aaaaatttaa ctggcatcag tcctaaaaaa attgatttca tccgcaggct
     7321 attgacagaa taattcagac tggtctttca ggcatccaga cacgctaccg cccctggctt
     7381 tttagctacc aatacactga ttgagcttaa tttttcaaac cctctctgca tacaatcgtt
     7441 gatgagaaag ggttattgcg gaaattaact tccgaatata aggtgacatt atggtaattg
     7501 aatattggct cgccaataat gcaggaggaa gtgttacagc taacggaata gcaggcaagg
     7561 taacaattcg gcaattggct atttttaaga attatattaa gtatcattca atatggtttt
     7621 taggagtttc tttaggttga caatatttaa tattgcgtct ccacatgcga tatttcttaa
     7681 ataatgtttt attattaccg cttttaattc agggataatg tgaggttatt accctcaaat
     7741 aatatgagat aaatagtgct gcaacattgc atttttgccc cgatttatcc atgatcgaat
     7801 tgtgacattt gtcatacaac gaataggttt tgtacttact atggaatgga ttgccgatcc
     7861 gtctatctgg gccgggttaa tcacgctgat tgtgatcgaa ctggtcctcg gcattgataa
     7921 cctggtcttt attgccatcc tcgccgaaaa actaccgccg aagcagcgcg accgcgcacg
     7981 ggttaccggg ctgctgctgg cgatgttaat gcgcctgtta ctgctagcgt caatctcctg
     8041 gctggtcacc ctgactcaac cgctgttcag cttccgctcg tttaccttta gcgcccgcga
     8101 cttaatcatg ctgtttggtg gtttcttcct gttgttcaaa gccacgatgg agctgaacga
     8161 acggctggaa gggaaagaca gcaataatcc cacacaacgc aaaggtgcga agttctgggg
     8221 cgtggtgacg caaattgtgg tactggacgc cattttctca ctcgactcag tgattaccgc
     8281 cgtcgggatg gtcgaccatt tactggtcat gatggccgcc gtggttatcg ctatcagcct
     8341 gatgttgatg gccagcaagc cgttaacgca atttgttaac agtcacccga ccatcgttat
     8401 tctctgctta agcttcctgt tgatgattgg ctttagcctg gtggcagaag gtttcggctt
     8461 cgtcattccg aaaggctacc tgtacgctgc cattggtttc tcggtgatga tcgaggcact
     8521 caatcagttg gctatcttta accggcgacg ttttctttcc gctaatcaga cgctacgcca
     8581 gcggactacc gaagcggtaa tgcgcctgct tagcgggcaa aaagaagatg cggaactgga
     8641 cgccaaaacc gcgtccatgc tggtggatca tggaaaccag cagatcttta atccgcagga
     8701 acggcggatg attgagcggg tacttaatct taaccagcgt accgtaagca gcattatgac
     8761 gtcgcgccac gatattgagc atatcgatct caacgcgccg gaagaagaaa tccgccagtt
     8821 gctggagcga aatcagcata cgcggctggt ggttaccgac ggtgatgacg cagaagattt
     8881 gctcggtgtt gttcacgtta tcgacctgct acaacagtca ctgcgcggcg aaccgctcaa
     8941 cctgcgggtg ttgattcgcc agccgctggt gttcccggaa accttgccgt tgttaccagc
     9001 cctggagcag ttccgtaatg cccgcacgca ctttgctttt gtggtggatg agtttggctc
     9061 ggtggaaggg attgtgacat taagtgacgt cactgaaacc attgccggta acttaccgaa
     9121 cgaagtggaa gagatcgacg cccgccatga tattcagaag aatgcggacg gttcctggac
     9181 ggcgaatggt catatgccgc tggaggatct ggtgcaatat gtgccgctgc cgctggatga
     9241 aaaacgtgaa tatcacacca ttgccgggct gttgatggag tatttgcagc gtattccaaa
     9301 acctggcgaa gaagttcagg tgggggatta tttgcttaaa acgttgcagg tggaaagcca
     9361 tcgcgtgcag aaggtgcaga ttataccgct gcgtaaggat ggcgagatgg agtacgaggt
     9421 gtgatggtat cggccatttt acccaatgct gcgtaacgcc ttataacttc agcaatgaag
     9481 cgttgtctgt ggtaaacgga catcacagcg ggggtgtggg tccggctgta aatcgccgaa
     9541 tcgtttcctg taggctgggg cagcccacat ggatgtgggc tgagggcgcg ttttacaggg
     9601 atgttacctc gcgcccgtac ccgatagccg aaaggaataa gatgagggaa ccgcgccagc
     9661 ggcgatttac cgccgggagc ccgggttgcc agggtggtgg cgattgagcc accctggcac
     9721 gttcaccggc ttgagcgttc catagtagcg aggaacatca agtgaacgga acagccttcg
     9781 atcttacata ttcagaatat aatagtgccg ccgcgaacgc ctgatgcgac gctttacgcg
     9841 tcttatcagg cctacaggac aggtcacctg gtaacctaca tcttctccag caacttcttc
     9901 acatctttgc cattgcggga atctttattc cgctcggccc agtcattcag gcgacgtttc
     9961 gcttcgtcct gtagatgttt gcgcagtaac tgatccactt gcaggctgta attgagttgc
    10021 tgccagttgc cataaacccg cagcggtact ggcgtttctt tgaggaaatc aatcagtttg
    10081 ctttccccgt tccagccacc cacgacccga atatcaaact gggtgtcgca ggtttgatct
    10141 gccagattca acatgccttc ccctgtcagc gccagcacag gcgattgacc ttgcatgtcg
    10201 tttaacgtca cgacgccatc cttcaacgtc aaatcggtgg taaagcggtc aagccgcgtc
    10261 acgttatcga agttttcagc ggccttcaca tcaccgccat tacgctctac cgcttgctga
    10321 atcatctgct ggaagttcat cccttccata cgtgtgtcgg tcatctcaac atgcgcctgt
    10381 ccttgccagt tgtggcggaa tgcgtcggca tctatgtcag caccggagaa atcaccagcc
    10441 agtgacattt ttccggtcaa cgaaatcgga tagttaaacg ccttcagaat ggtaccaatc
    10501 tcaacgttct ccagccgtgg ctggaagttt atccgcggat ttattgatgt cgcgtccagc
    10561 gtgcccggca gtgaaacctt tccaccgtta agcttgccct gcagttgagt aatttccagc
    10621 aaacccgact tgttggtcat ttgcgtggca acatctgtaa aattcattcc gcgccagcgc
    10681 acgttactgg cctgcaacaa aatatcagct gtaaagcctt gcagtccctg ataggccggt
    10741 tcatcaatac gcgaagaaat gaccgggcgc ggcaacgttg attggctctg cccctgctgc
    10801 gcggcaccgt tttcaccatt cgcggtttca ttaagcggga tgaggttgtc aagattcagt
    10861 tgcgggaatt gcagtctcag ctgccattcc ggtttctctg tcagcgtgac ctgtgcttgt
    10921 ccgctcagcg tactgtcatt ggcggtcaaa ctaatttggt taaatgaaag gcgtttatgt
    10981 gactcctgcc actgggactg gaaactcccc tgcccctgaa taccttgttt tggtaaatcg
    11041 gcaccctgca actgccagtt aatttgttca atagccgctg ttaaatcatg cggataatca
    11101 gaagcatcta ccgtaccgtt aagggatatc gtgagatcgc gctgatcgcg attaacccgc
    11161 ccggagaact caaatgagcc acgatgttgg ggatcttgtt ccatttgcag gcggatattg
    11221 cggattgtca cctgctcgtc atcttcatgc tggaacacca gaacgctgtc cgccacctta
    11281 agactggata tatcaaacga ccatccgcga tcgtctgaca gatccggcaa ggtattgtcg
    11341 cgcggtgcaa ccggagcgtc ttcactgcgc accgcttccg tctgcggcgt cagttggatc
    11401 actgcccctt ttagcatcac ctgcttaacg ctcagttgat gactcagtag tggtaaaagc
    11461 gccacgtcca gacgcatgtt gtcggcgcga accagtggct ggcttgcgcc ctgggcggtg
    11521 agagacattc gcccggagag gatactaagc tgcggccaga cgtgccaacg cagtggcccg
    11581 tcgagctgca attgataacc gctacgtgca gcaacttgct tgaccatata gtcgcggaaa
    11641 tcattcggat tcaccagcaa cactaacgca gataacccgg ccaccagcac gaccaagagt
    11701 atcatcagcg tcgtcagaaa tcgtctcatg gtatcctcaa tgggcctgaa ttagtcttta
    11761 tcgattcggc tggctaccgc gccctgctgg ttgcgatatt tcgcatcttc acggcggttg
    11821 taaggtcgcg ccgccgggcc ggaaagcggc tcaaagctca gcgcaccaat taacatgccc
    11881 ggacgcagcg ccagcggcag cttaccggag ttgtagaact ccagcacaat gcaaccagac
    11941 cagcccggat cgatacggtg cgcggtgacg tgcaccatca gccccagacg cgccaaagag
    12001 gaacgcccgt ccagccagcc caccagatcg gctggcagcg tcaccgactc cagtgtcacc
    12061 gccagcgcca gctctcctgg gtgaagatag aacgcctcgc cctcgtcgag aacgatctca
    12121 tcgctcatca cgcggtcaag cgcggcgctc acttcatctt tgggaccgct cagatcgata
    12181 aacgctgccg tgtgaccacg gaaggtacga aatttattgc ccaggcgtac atccaccgtc
    12241 gcgccgttaa tacgctccac tggcggacgt gggttgatcg acaaacggcc ttcatcaagc
    12301 caggcttcaa tatctcggtc acacagacgc atggcatttt ctcctttcgc acatcactcc
    12361 cttaacgcca attacgtcaa gggcatacca acttatcact gaacggtaca caatttatca
    12421 agcttattca aagaactgac ttattttcgc tttcaatata tcgatcgcga tgcggttttt
    12481 cccgccgcgc ggcacgataa tgtccgcata ttgtttagaa ggctcaatga attgcaggaa
    12541 catcgggcgc acggtttttt gatattgcgc catcacggaa tccattgaac gcccacgctc
    12601 gttaacgtca cgcttgatgc ggcgcatcag gcagatatcc agcggggtat caacgaaaat
    12661 ggagaagtta agttcgtcac gcaaacgcgc atccgtcagc aacaaaatgc cttcgagaat
    12721 gatgaccttc ttcggctcaa ccgtcaccgt ttctttcata cgcgtatgtt caacatagct
    12781 gtaaaccggc aggtcaattg ccgagccgcg tttcaacgct tgtaaatgct caagcaacag
    12841 gctgtgatcc atcgcgctgg gatggtcgta gttggtctta acgcgttctt ccatcgacag
    12901 atggctttga tctttgtaat agcagtcttc gggaattacg ccgatgtgtt catcaccgac
    12961 ttgctcacgt aattcacgat aaagggtact ggcaataaga ctcttgccgg aagccgatgc
    13021 gccagcgata ccgataatga cgcactgatg agactgatca gtcatatatt tagcgacctg
    13081 attaacctgg atgttaggaa gggggcgacg aagcgccaaa cgcggcaatt atagggattt
    13141 catccgtctg ataccagtcg aatagcgttg ccgcgcgctc agagttaatt gttgacaaag
    13201 aattcccggt ggcaaattac gttgatcagt tttatgtaag gtaaaaaatg ttatacgcag
    13261 ttgcgcaaat tatccgcctt tacgtcactt tatgagcaat tcgcatataa aatgtaaaac
    13321 ttttgtacta gcataaacac agaaacgaat actggcgacc tggccttgcg gataaagcgg
    13381 taataagcaa acaatcacag catgtattaa ttgccctgcc ccacccgctg cttcacctgg
    13441 tcagtttagg tttagtctcg tttatcttta cccttgtctc gcttgagctt tcgcagtttg
    13501 gcacccaact cgccccactg tggttcccga cgtccatcat gatggtggcg ttttatcgcc
    13561 atgccgggcg catgtggccg ggaattgcgc tgagctgttc gctgggaaat atcgccgcat
    13621 ccatcctgct tttttccacc agctcgctga acatgacctg gacgaccatc aatattgttg
    13681 aagccgtggt cggggcagtg ctgctacgta aattgctgcc gtggtataac cccttgcaaa
    13741 atctggctga ctggctgcgt ctggcattcg gcagcgccat tgttccgcct ctgttgggg
//
LOCUS       DAEEGA010000098        13532 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEEGA010000098 DAEEGA010000000
VERSION     DAEEGA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13532
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.098"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..890)
                     /locus_tag="KLH46_003291"
     CDS             complement(<1..890)
                     /locus_tag="KLH46_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0379876.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="KLH46_003292"
     CDS             1131..1931
                     /locus_tag="KLH46_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBE0379877.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="KLH46_003293"
     CDS             2009..2779
                     /locus_tag="KLH46_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE0379878.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="KLH46_003294"
     CDS             complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="KLH46_003294"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBE0379879.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="KLH46_003295"
     CDS             complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="KLH46_003295"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBE0379880.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5046..5768
                     /locus_tag="KLH46_003296"
     CDS             5046..5768
                     /locus_tag="KLH46_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE0379881.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="KLH46_003297"
     CDS             complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="KLH46_003297"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBE0379882.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6297..7028
                     /gene="dnaQ"
                     /locus_tag="KLH46_003298"
     CDS             6297..7028
                     /gene="dnaQ"
                     /locus_tag="KLH46_003298"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBE0379883.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAVF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7161..7237
                     /locus_tag="KLH46_003299"
     tRNA            7161..7237
                     /locus_tag="KLH46_003299"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7195..7197,aa:Asp,seq:gtc)
     gene            complement(7368..8414)
                     /locus_tag="KLH46_003300"
     CDS             complement(7368..8414)
                     /locus_tag="KLH46_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HBE0379884.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="KLH46_003301"
                     /pseudo
     CDS             complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="KLH46_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9407..11290)
                     /gene="tssF"
                     /locus_tag="KLH46_003302"
     CDS             complement(9407..11290)
                     /gene="tssF"
                     /locus_tag="KLH46_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBE0379885.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11306..11800)
                     /gene="tssE"
                     /locus_tag="KLH46_003303"
     CDS             complement(11306..11800)
                     /gene="tssE"
                     /locus_tag="KLH46_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBE0379886.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11797..12627)
                     /locus_tag="KLH46_003304"
                     /pseudo
     CDS             complement(11797..12627)
                     /locus_tag="KLH46_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12614..13528)
                     /gene="tagK"
                     /locus_tag="KLH46_003305"
                     /pseudo
     CDS             complement(12614..13528)
                     /gene="tagK"
                     /locus_tag="KLH46_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3101 a         3522 c         3611 g         3298 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcgc tactgcctgc accaatcgtc aaactttggc ctggcttcag
     3061 cttagatccg cgcagtttgt tccactgctg caaatctttg gtgcttacgc cgagacgtga
     3121 agcgatgctt gaaagcgtgt cgccagagcg tacggtgtaa acacggctgt taagcggcgt
     3181 attgttggca accagcgtcg actgtacagc agcaatttcg cctgaagcga gagattcacg
     3241 cagttgatct gcatgcttct ttggcaccat cacgtactgc ggcccacttg cgcccagcgt
     3301 ggagcctttc acgccagcgt tgaatgtctt cagcttgctg acggaaatcc ccgccatatc
     3361 tgcaaccttc gccatttcaa ccgggctact caggtgcaca cgcgccagag cacggctttc
     3421 atcggtcgtt ggcagacgta cgccataacg cttgctgttt ttgagaatat cactcaatgc
     3481 cagcatttta ggcacgtact gcttcgtttc ctgcggcaac ggtaacgacc agaagtccgt
     3541 ggatttccca cgcgctttgt tcgttttaat tgccttcatg acccgacctt cgccgctgtt
     3601 ataagccgct acggtcagaa gccagtcgtc gtcaaacatt ttgttcagac gctgcatcat
     3661 attcagcgcg gcagttgttg aagcaacaac atcgcgacgc gcgtcataat tgcgggtctg
     3721 tttcaaacca taattgcgcc ccgtgctcgg aatgatctgc cagatgcctg cggcattggc
     3781 gccagacgtt gcgtgaggat caaaagcgct ctccactatg ggtagtagta ccagttccat
     3841 aggcatgtta cgttttttaa cttgccctgc tatccagtac atatacggct ctgcccgtaa
     3901 agttacatcg tggagatagc tcttattgcg taaatatttc tgtttctgtt cgcgaatccg
     3961 gtcattttcc ggaattccca tctttagctc gtcgccaatg aaagcccaca agtcaccatc
     4021 tggcgcgata gacgtcccat cgtccatcca tcgtgcctga cttgtaaact ttgctgcttc
     4081 cccttgacca gctgcagaaa ggctctgtgc gtgctgttga acgttgccgg tactctggca
     4141 acccacgagc aggacagagg cgagtaatat cgcttttgcc ttcatgtgtg tgtcaatagt
     4201 tgcttaaaag acgaccgatc ataacggcga acggagccga tgacaagaaa gttttatcag
     4261 aacctatctt tctttgacct taaccatgca aaacgctctt caggttgttg caataatgtt
     4321 tcttcattaa ttccattaat taaatcaata tcttccgttc ttaaaaaaac attaatttgc
     4381 cgctcatttt tcagaattac gggtagtgtt atttgatttt ttgcccgtaa ctccttaact
     4441 ttacgataat aatcatttat ggacaaatcg tgcggaaaaa tactcaaagc aaacttcata
     4501 tttgataacg tatattcatg agcacaacat accaatgtat cgtcaggtaa cgcacttagc
     4561 tttttaagtg attgatacat ttgtaaagct gtcccttcaa acaaccgacc acacccacca
     4621 gaaaacagcg tgtcgccgca aaatagataa ggtttactga agtaacagat atgtcctaaa
     4681 gtgtgacccg gtgtggtaat tacactaaat tcatgcccta aaacgaaggc agtttcgcca
     4741 tctttgacta cctgtgttgt tcccttatct tgtgtctctt gtggaccata caccacaatt
     4801 tgcggaaact tttccaccag ttcttttacg ccgccaacgt gatcgtggtg atggtgggtt
     4861 agaaatatgg cctccggttg ccagttattg gcggtaatgg cgtttaatac tggctctgcg
     4921 tctccgggat cgacaatcag gcagcgacct gcctcatcat tcaaaaccca gatgtaattg
     4981 tcatcaaagg cgggaatact gttaagattc atagattacc tctcagtgta aaacggaagg
     5041 ttgtgatgaa accggcaaga gtccctcaaa ctgtcgtggc tcctgattgc tggggcgatt
     5101 tgccctgggg agagctttat cgcaaggcgc tggagcgcca gctcaacccg tggttcacta
     5161 aaatgtatgg ttttcatctg cttaagattg gcaatttaag cgcagaaatc aattgcgaag
     5221 cgtgcgcggt ttctcatcaa gtgaatgttt ctgcgcaagg aatgcccgtc caggtgcagg
     5281 cggatccact tcatcttcct tttgccgata aatccgttga tatttgtcta ctggcacata
     5341 ccttgccgtg gtgcaccgat ccgcatcgtt tattacgtga agccgatcgg gtactgattg
     5401 atgatggctg gctggtgatt agtggcttca accccatcag tttaatggga ttacgcaaac
     5461 ttgtgccggt attgcgcaaa acctcgccct ataatagccg gatgtttact ctgatgcggc
     5521 aactggactg gctctctttg ttgaattttg aagtgctgca cgccagccgt ttccacgttc
     5581 tcccgtggaa caaacacgga ggaaaactat tgaatgcgca tattcctgcg cttggttgct
     5641 tacaacttat tgttgcccgg aaacggacta ttcctttaac gctaaatccg atgaaacaga
     5701 gtaaaaacaa gccacgaatt cgccaggcgg ttggagccac ccggcaatgt cgtaaaccac
     5761 aggcttaaac ttcgacttgg tagcccgtat cttccagtgt gggattcatc gccgcggcac
     5821 gagccagttc atcacagcgt tcgttttccg ggtgtccggc atggccttta acccattccc
     5881 atttgatttg atgctgcccc aatgcagcat caagacgttg ccagagatcg acatttttta
     5941 ctggtttttt gtctgctgtt ttccagccac gttttttcca gttatggatc cactgggtga
     6001 taccctggcg gacatactgg ctgtcggtac tcaaaatgac ttcgcaatgt tcttttaacg
     6061 cctccagcgc gacaatagcg gccatcaact ccatacggtt gttggtggtg cgggtgtagc
     6121 ctgcgctaaa ggttttctcg cgtccgcgat agcgtaaaat agcgccgtaa cccccaggtc
     6181 caggattacc cagacacgaa ccatcggtga aaatttctac ctgtttaagc atctctggta
     6241 gacttcctgt aattgaatcg aactgtaaaa cgacaagtct gacataaatg accgctatga
     6301 gcactgcaat tacacgccag atcgttctcg ataccgaaac caccggtatg aaccagattg
     6361 gtgcacacta tgaaggccac aagatcattg agattggtgc tgttgaagta gtgaaccgtc
     6421 gcctgacggg caataacttc catgtttatc tcaaacccga tcggctggtg gatccggaag
     6481 cctttggcgt acatggtatt gctgatgaat ttttgctcga taagcccacg tttgccgaag
     6541 tagccgatga gtttatggac tatatccgcg gcgcggagtt ggtgatccat aacgcagtgt
     6601 tcgatatagg ctttatggac tacgagtttt cgttgcttaa gcgcgatatt ccgaagacca
     6661 ataccttctg taaggtcacc gatagccttg cggtggcgag gaaaatgttt cccggtaaac
     6721 gcaacagcct cgatgcgcta tgtgcccgct acgaaataga taacagtaaa cgaacgctgc
     6781 acggggcatt actcgatgcc cagatccttg cggaagttta tctggcgatg accggtggtc
     6841 aaacgtcgat ggcttttgcg atggaaggag agacacagca gcaacaaggt gaagcaacaa
     6901 ttcagcgcat tgtacgtcag gcaagtaagt tacgcgttgt ttttgcgaca gatgaagagc
     6961 tggcagcaca tgaagcccgt ctcgatctgg tgcagaagaa aggcggaagc tgcctctggc
     7021 gggcataaat acatgtaaaa ggcgctaaaa atagcgactt gggcgatttt tgcagcaaac
     7081 gattcaaaag atgagaaaaa tcgttgacga aggtcgaggc aatccgtaat attcgcctcg
     7141 ttcccaacgg aacacaacgc ggagcggtag ttcagtcggt tagaatacct gcctgtcacg
     7201 cagggggtcg cgggttcgag tcccgtccgt tccgccaata ttcagaaagc ccgaatcagt
     7261 aatgattcgg gctttcgtcg ttttggtgaa gtgagaattt ttcactccga agtaaaacgg
     7321 atatgattta aaaaatggcc agacattata aattctggcc acttctgtta ctccagcgca
     7381 tcccatttct ccagcagttc tactggaatt tcatttatta cctccgagac gcgttttccg
     7441 accaggcgtt ctgcctggcg cagcaaagga atggtcgggc tgctcggttc gctgtgctca
     7501 aaccagcgac ggatgacacg caggcgttcc agcgcgtcgt ttcggtcgcg gatctgccgg
     7561 ggttctgcgc cggtcatcgg tgatatttcc tccgcgatat gcaccggtag cgcaatctgc
     7621 tctgcctgca tctgttcagt atgaacgatg gtggcgtttt ccggctgctc agcctgtggt
     7681 tgtggcagga tctccggcgt ggtgctctgc actgctcccg gtaacagggc cagcagttgc
     7741 gtcagacggg agaagtccgg tgcctggtca ttcagcgttt cacgcgcatg gcgttgcagc
     7801 cgctcagtgg tctctgctgc ctggcgaaag gcatccagcg gcagtgtgcc gcgcgcttcc
     7861 aggtctgcca gttgctggcg taccgattct ggtgccagcg catccgccgg gcgtaatgca
     7921 gatagcgaac gctccacatc ccgcacctga aggcgcatgg cagcattatt tgacagtgta
     7981 atgccacgaa tatccgccat tacaccttcg tggtccagca acgccgccag cgcgttactg
     8041 cgtgccaccg cagcatcttc ggcggtgata tcttcagtcg cgagcagttg aggatggatg
     8101 gcgtccgggt acgtggagca caacgtttcc agcagcgtga gcgcctccga cagtcccctg
     8161 gcaccggcct gctggatacg gcagcgcagc aggacaacca gaatgcggat atctttgctg
     8221 cgcatcagca gacgatgggc gtcccgctca agctccgccc agttaatgtt ttccggcgta
     8281 ctgacaaaat cgccatattg cgcttcttca cgcggtgcgg cgcgggtgaa cagcagcagg
     8341 tattccggat cgtactccag atccgggccg caggggcatt ccgccgaaat tgggtcagac
     8401 aggaatattt ccatttaatt atcctcagtg cgccaggtac tgttctggtt cgaaaatcat
     8461 gcccgtcacc gggttgtcgt gcatggcttt acccgcccat gcagtccaac ccagttggta
     8521 cggaccaccg gtaacagccg gaggggcggc gtggggcttt agctgtagct caatttccca
     8581 gcagtattca taaccactaa aggcgcggac cagttctgtc aggacgggca ggttattgct
     8641 gccaggcaga aaacgtcggt aatcctccag cgtcagcggg ccgataacca gccggaactt
     8701 atactgcatg tccggcacgg cctggccgat gagtgcacca ttgccaataa cggaagaggg
     8761 ggcaggcatc cccagccggg tgatctcatc gtccgccacg gctatccagt gcaggacata
     8821 ctcctgtatc cggaaaggta caccgaaata gtgcgccagc gtggtggcca gaccgtccgg
     8881 attgcgggac tcacgcacca gatgggccga tgccgccaga cgagcgtgat ctggcaacgg
     8941 gctgtcagcg ctctcccgca aatcctgccc gctgaggctg gcgacataaa aggcaaagcg
     9001 gtcatgttgc ggtttatcca gaccgccccc agcggactgg gcgctgcgcc atgcctggta
     9061 aaactgcgtc agccagcggt gatgaaaaag atcagaaaaa cgggtcagcg tcggctttca
     9121 gtccggttac gcgccagttc ggtgtagtgc agcggcagtg gaccgttcgg cccccacagc
     9181 ccgaggctgt acagactgat gtgcaggcgc ccttcctgcc agcccacact ggcgatttct
     9241 cgtggcgcaa aggtcagcgc gggcgtttgt ccgaggcgaa atttttccat ccgtggttgc
     9301 caggtgttgc ccgccggaac ggtacacaac tgcacatcca cgcggcgcat caggttcaga
     9361 aaaccgtaac gccagggggg ggcctgcgcc tgagtgaaat cgttgctcat acgcttcccc
     9421 gctgtcccgc cctgacaggc caggtcataa ttttgccgcg ctgcatgctg tgcagcgtca
     9481 tctgcgagaa ggtattaatg gaaacatggc gggcgatata gtgttccagc accaggccga
     9541 acagatacgg actgatgccg gaaaaacctt cttcatccac cgtcagttca caactgacgc
     9601 cacgcccata gaccagtagt ccggaaccgg gcagacggcg ggtgaccggg gtcgttttac
     9661 aaccaatcag gctgcgcacc tggcgcgatt gcggactgtc atgagcagga ataaacaggt
     9721 tcagcagatc tcgcaacgcc tggccgccgg tacggtgatc cagatcggcc agcggcagat
     9781 aattaaaaga taactggcgg atcaaccgcc atgccatctc acgttcagcc agcgggggct
     9841 gcggcgagcg tggcgggcgg ataagcccta cgcccgccac cgggatggct gcatccacgg
     9901 ttagatcgtc ccggccattg cgggcaataa gacagggcag atcgcggtta gtgaccatgg
     9961 ccgtgatggt gatgtggcgc agattttccg ggtacggcgc ttcatactgg tcaaccagcg
    10021 acaggaacac ttccgagccc gtataggggg tacgggtgcc gtaacggcgg gcgttttctg
    10081 ataagcggcg gggctcccgg cgcagggaaa aataacgtcc gtgattgcct tcatcattat
    10141 tgcgggtgtg gtagagcggg cgaaaggcca tcttccgtgt ggtgtcggtt tccagccctt
    10201 ccacttcctg gaccgagaac acctcataat ccagcggatg ggttctgtcc accaccagat
    10261 gctgttccgt cgtgctgtgc gtgacatcga tccgggcggt gacgcgggga aacaggttga
    10321 taaccggggt acagaacagg ctgaactgtg ccgcatttgt ctggtggatc agccagtccg
    10381 gcggcaggcg gttcagcagg ataacaatct ccgccacgcc accgtcaatt ttctgtaatc
    10441 cggcagacag gcctgtcggg gtgaaaaaat agaagcgttc cggacaggca aaatattcat
    10501 gcagcaggtt atgaccgtga aacacgttcc acgccagcgg cagcagcccc tgatccggct
    10561 ccaggccttc gtacataacg ggctgttgca gattcacatc cagcgcgccg tcaaaatgcc
    10621 ccggaatgcc agccagggtc gcgatggcgc tggtatgtag aagttctaac aggtgtgagg
    10681 ccgtacgctc ctcgccgcag agataaaacg gcagcctgtc cagtccggca agctgactga
    10741 acgttagttc accaaaggtg cgcagggtaa tacgcagcgc cccggcgacg tgaatgttgg
    10801 ccggcaggta gcggtgcagg gcaggcatat ccggtggcgc ggcggtcagt cgcgcctctt
    10861 cgatggcgag cggccacagc gttacatcct ggctgctgcg aaactggcag gcagtgcttt
    10921 cgccttccgg aatggcggag aaaaaggctg tgtcgcgcgg gacggtaaat cctttcgcca
    10981 gatcgccttc ttccgtatcg ggatgcaact gcgccacgct cattgagggc gtgggtgtga
    11041 cgtaattggg gctgaccact tccagcaggc gctgggtgaa acgggggaat tcagcgtcaa
    11101 tttttagctg cgtgcgggcg gtcaggaagc tgaaggcctc gaccattcgc tctacatacg
    11161 gatcggcaat atccgttccc tgcatgccca gccgggcggc gactttggga tgacgggcgg
    11221 caaattcggc tcccgtttcc cgcagatagc tcagttcgcg gttgtagtat tccagtagcc
    11281 gtggatccat cggtctctct ctgaattaaa aaaacgtaat gcgactctgc tccatatcca
    11341 gcgcgctacg tacccggaat gcagtcggat acggctgcgt gaggatctcg ccgcgaattt
    11401 caaactgtaa tgtgttatgg ccactctggc ggcgctcatc ctgaagtggg atgatttgca
    11461 gagtggacgc tttcagccgg ggttcaaacc gggtgatggt tcgcaaaatg gccctgcgga
    11521 tatcgtccca cttgtgctca tacagaaagc tgcccgccag tggcggcagg ccgtagttaa
    11581 gtacggacgc tgccgcgtac ggataacgtg cagcatcaat atcatcttca tggctgacgg
    11641 tgttgagcag aaatgacaaa tcccggcgga tgatctcctt aatctgaacc ggactgacgc
    11701 tgataccgtg gtcgcgtttc tggtttgggg cgtcgtcaca caggcggtca aacagcgtgg
    11761 gcagcaggtg attgcctgga cgctggcggg aagcgctcac gcgtcgcttc cttcagcggt
    11821 gttaaaggta caggtggaca aatccagcag gctgatatcg ccgtgaccgg tgagccacac
    11881 cctctgaccc agcgcctgta ccagtgtctc tcccggaccg tcctgccaga ccgtttcccg
    11941 gcacagacgc agggagtcgg cagccttttc agagccgctg tagcggggaa acagctaggc
    12001 actgtgtgta tcgccattgt tcagggtgac gttgacgggt ttccagatca aatcgatcag
    12061 atgcgcaggc cgcggcgatt tcagcgaaca gatttgtgaa aacggcagcc agatatatgc
    12121 tccgcccgcg atcagttcga gtaccggacc ggtacgggag tcgctgtcgc taatccagtc
    12181 aaatgcgccg ccattccact gcccggaagt gtctgttatg gcctcaagcg cgcggctgcg
    12241 gtgagcatca gcttcctgcg cttcaccacg ggcgttacag gcgagggcgg tcagcagatc
    12301 ctccatccag gcgggcggcg ggagaataac accgggccgc tgttcgccct gaaagcaggc
    12361 gtggcggtat atctcacagc ggatcagctc gccaaatacc tgcgcttcgc gagtataatt
    12421 tgcgtccatc cgggcgcata gctggatctg ttgcagtgcc cgcgaccagt cgcctgccac
    12481 gcacaataac tgaaacaggc tgtggcggcg ggaggcctgc gctgggtgca tcctgacctg
    12541 ttgctctgcc atctgaatgc cctccaccac ggaataatcc cgcagcagcg ccgacaaggt
    12601 aatcggtagc tcgttattgt ttgtcatgat atatctctcc atgttgtgcc gggtttatcc
    12661 ggtaatggct gtcaatgcca atgatgcgat gctctttccg tgtcagatct ggcagtacgg
    12721 tgttatgcgc tgtttccggg gcgtattccg gggaaagtaa atgcaggata tcagggggag
    12781 tcttttcgac aggccagata gtttcatctt cgccagtggc atccagtaca ttgagaatag
    12841 cgtcaatgct taacccacca gtgaccatat cctgcaatgt ctcggcttcg gtgaccaggt
    12901 cacgcaatga atccagatcc tgcgaggagg gctcttcgtt aaaaggaaaa ggatcagcat
    12961 ggcgattaag atcttgttcg ggagataaca acgcctcgcg gtattcctga taaagtcggg
    13021 tcagggcatt tcctttttcc gcaccttctc cggtggagga ggctccatga acaggaatga
    13081 tatcgaatgg attttgtgat gtcagatgct gctgcctgaa ccaatccaga tccagatatt
    13141 cggttattgc ttccgacgtg atggcagtct gcgatatcgt ttttactgtg ctcaggttcg
    13201 gagatgatgg attgccatgt acaaggcact atgaagataa cccccattct atcaggtcgc
    13261 cttcattcag acgcatacgg tgatgaggtt ccacaatctg gtcgtttacc gtacagcaat
    13321 attcggtaga ctgattgata atccaccatg cttcttcgtg gcgggtcaga gtgagggcaa
    13381 tctttacatc atcattcacc ttaaaaacag gaatccaggg agaggtaacg gtgaagataa
    13441 tttgtgttcc ttcagtcgcg taaattgtgt tatcggcgat cccgtgcgcc tgtatttttt
    13501 ggagtcccca cgtaatattt ggtttcattg tg
//
LOCUS       DAEEGA010000099        13015 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEEGA010000099 DAEEGA010000000
VERSION     DAEEGA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.099"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..319)
                     /locus_tag="KLH46_003306"
     CDS             complement(<1..319)
                     /locus_tag="KLH46_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBE0379887.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFD"
     gene            complement(525..1244)
                     /locus_tag="KLH46_003307"
                     /pseudo
     CDS             complement(525..1244)
                     /locus_tag="KLH46_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="KLH46_003308"
     CDS             complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="KLH46_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBE0379888.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="KLH46_003309"
                     /pseudo
     CDS             complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="KLH46_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
     gene            3155..3838
                     /gene="cusR"
                     /locus_tag="KLH46_003310"
     CDS             3155..3838
                     /gene="cusR"
                     /locus_tag="KLH46_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBE0379889.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3828..5270
                     /gene="cusS"
                     /locus_tag="KLH46_003311"
     CDS             3828..5270
                     /gene="cusS"
                     /locus_tag="KLH46_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBE0379890.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5420..7657
                     /gene="nrfB"
                     /locus_tag="KLH46_003312"
                     /pseudo
     CDS             5420..7657
                     /gene="nrfB"
                     /locus_tag="KLH46_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            7644..10612
                     /gene="nfrA"
                     /locus_tag="KLH46_003313"
                     /pseudo
     CDS             7644..10612
                     /gene="nfrA"
                     /locus_tag="KLH46_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10613..11503
                     /locus_tag="KLH46_003314"
     CDS             10613..11503
                     /locus_tag="KLH46_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBE0379891.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11686..12447
                     /gene="envY"
                     /locus_tag="KLH46_003315"
     CDS             11686..12447
                     /gene="envY"
                     /locus_tag="KLH46_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBE0379892.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQREKVMW"
     gene            complement(12493..12569)
                     /locus_tag="KLH46_003316"
     tRNA            complement(12493..12569)
                     /locus_tag="KLH46_003316"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12533..12535),aa:Arg,seq:tct)
     gene            12812..>13015
                     /locus_tag="KLH46_003317"
     CDS             12812..>13015
                     /locus_tag="KLH46_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBE0379893.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3183 a         3373 c         3307 g         3152 t
ORIGIN      
        1 tgtcgaacca gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa
       61 aataagtgcg tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc
      121 tggcatcctt caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat
      181 gccagcgata gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc
      241 catcagcatc agccacttct tgtgggccag aatatggttt accataaata gtacgcagtt
      301 ttgtgtcgct tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac
      361 atctggtaca caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg
      421 tagacatact caggtgaaaa aagggttgtt tttcgagtta taacagacag ttaattaact
      481 acagtggaca atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg
      541 accacgttat ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca
      601 tcaatgggcg ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga
      661 atatccgcct ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc
      721 gccgtaccgc cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga
      781 atggtcaggt cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac
      841 accttgtcga taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa
      901 ctgacattcg ccgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt
      961 ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct
     1021 tcatcggcat atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg
     1081 aaccgcgtat tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc
     1141 attttttctg tgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata
     1201 ccgcccgcga tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt
     1261 tactggctga ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac
     1321 gccactttgt cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc
     1381 atggtcatct ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg
     1441 ctttccagat caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg
     1501 ctcatggttt catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga
     1561 ctgaacattg cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt
     1621 gctgccaacc gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat
     1681 tcaggtcaag taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca
     1741 gataacttac cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg
     1801 acgccagata acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg
     1861 catctgccac ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct
     1921 gctggcgaat ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga
     1981 tctcaatttt aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat
     2041 cgctgctggc ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg
     2101 cagcaccaat attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc
     2161 gctgcaataa gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc
     2221 tgtttactgt ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat
     2281 tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca
     2341 ccccgcgagc ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga
     2401 caaacgcata tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat
     2461 acgccagctg ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg
     2521 cgcgctgggc ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta
     2581 agcgaccgaa aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg
     2641 tggctgaatc gcctttaaga ttgccgctcc agctgccgct gccctagcca ttgagctgtg
     2701 ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg
     2761 ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat
     2821 caacaaaaaa ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt
     2881 tctggctgag tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca
     2941 gtgaacaacc agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag
     3001 gctcataatt tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg
     3061 tgacaggaaa atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta
     3121 ctctagaatt gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga
     3181 aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt
     3241 ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat
     3301 cctcgatatt atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc
     3361 caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa
     3421 ggggctggag ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct
     3481 ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca
     3541 ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc accctcagcg gcacgcgcat
     3601 cactctgacc agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt
     3661 gctgccccgc tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa
     3721 cgccattgat gtggcggtga agcggctacg cggcaaaatc gacaacgact ttgagccgaa
     3781 gctgattcaa accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc
     3841 catttcagcg cccgttttcg ctggcaaccc gcctgacctt ttttatcagc ctggccacca
     3901 tcgcggcgtt tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg
     3961 agcaggacat taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc
     4021 ctgacgaaac gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca
     4081 acgtgttgat ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc
     4141 ctgatatccg cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg
     4201 tgtatctcct ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac
     4261 acagcaactg gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt
     4321 atacgctcta catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga
     4381 ataaacttat tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg
     4441 cggtacataa aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct
     4501 cgaaagatct cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg
     4561 tactgtcgtt caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt
     4621 tctcagcgga tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg
     4681 aaatcgccct cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc
     4741 tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg
     4801 ataacaacca gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag
     4861 tgttcgattt tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg
     4921 acaaacgtca ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt
     4981 ctaatgccct tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg
     5041 atcaccaggt gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac
     5101 cgcgattgtt tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta
     5161 gcggtattgg gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg
     5221 taacgtcaga tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca
     5281 aacggcaact tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg
     5341 gtaaaaagct gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc
     5401 gttaatgtaa ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg
     5461 cttaaaagta atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt
     5521 ttttattgat gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg
     5581 ctacccgcga atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat
     5641 ggttccggcg tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac
     5701 gctcgactac gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca
     5761 acgtgatgtt gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg
     5821 tcctggtccc accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt
     5881 tgaacgtagc gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat
     5941 ttcaccgatg gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat
     6001 cccagtgtat ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga
     6061 gttttcagaa ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc
     6121 cagcgcaggc gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg
     6181 taacggtatt gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct
     6241 gaaagaaaaa ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga
     6301 acgcgagcag cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga
     6361 atatttcccc gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat
     6421 tgttttccag ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct
     6481 ctggcgcgac cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat
     6541 gatccagctt ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct agcatttcct
     6601 ttctattttt agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat
     6661 ggttaaccgc atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg
     6721 cctgctttcc gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg
     6781 cgtgctgaaa caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac
     6841 gcatgacttc cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct
     6901 gctggaaaat caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga
     6961 aggtctacgc ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc
     7021 acaggcgctg gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc
     7081 gtcctcgctg attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct
     7141 gcgtctggaa aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct
     7201 ggcggccctg acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca
     7261 aattgtcaca gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat
     7321 gttgtacaat gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg
     7381 gcaatatgtg ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa
     7441 tcgttcagca attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa
     7501 gtttttggtc accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca
     7561 acgcgaatta caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca
     7621 ggttgcgcaa ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt
     7681 ctggtttact gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca
     7741 gcgcagaaga gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata
     7801 aggcgctgaa ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata
     7861 tacaccaaca ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc
     7921 attttggtca tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag
     7981 gagatgcccg acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga
     8041 ctgttgaaga actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc
     8101 gcagtgaagt cgggcagaat accctgcggc tggcacaatt acctgtcgcc agagcgcaac
     8161 tgaacgatgc gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc
     8221 aacgggcaat ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc
     8281 agcagaacac attaagcgcg gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc
     8341 agctggacga tcggatcctg gcactgcaat cacaggggat cttcactgat cctcagtcat
     8401 atattactta cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc
     8461 tcattgaaaa taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt
     8521 tatctaaata cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg
     8581 ataaccgcca gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg
     8641 acgcagcgca aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg
     8701 ctgtcagcgt ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc
     8761 agcaagaacc gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg
     8821 agcagtcacg cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc
     8881 gtgtcagcca gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag
     8941 caacgccggc gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt
     9001 ggcaaagtca gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg
     9061 atatgtcgcc ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca
     9121 cgctacccgg tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct
     9181 ggcaacatcg tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg
     9241 cggcctggca gaaaatcagt cttcacgaca tgagtaatga tgatctgctc gccgccgcca
     9301 acaccgctca ggcagcggga aatggcgcgg ctcgcgatcg ctggcttcaa caggcagaac
     9361 aacgtgagct gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag
     9421 ctcagccaga actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca
     9481 actcttacgt tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga
     9541 gtgatttgcg cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg
     9601 gttacgcctt gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc
     9661 ataaagggct tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc
     9721 tggatgacat gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc
     9781 aggcataacc ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca
     9841 tgaggaggtc ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg
     9901 cgcaatgagt accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag
     9961 ctacggacaa ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct
    10021 gctctcagtt tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt
    10081 gaaaaatccg atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt
    10141 cctcgccgtc gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct
    10201 gcgcgccagc gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg
    10261 ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat
    10321 tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac
    10381 tattgagcct tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc
    10441 gcagcttggc ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc
    10501 ctggccgcac aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca
    10561 acgtaacgga gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa
    10621 gttcattttc gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg
    10681 tattatctgg caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct
    10741 gatgagtcag ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg
    10801 cgatgcattt acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca
    10861 acagactggc ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca
    10921 gaaacagtcg tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca
    10981 gcaagccaga ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc
    11041 ggaaattgac gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt
    11101 aaacaacgcg cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt
    11161 tttcgccgga aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac
    11221 cggagttaat gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg
    11281 tgaacgttat ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt
    11341 ttatgaactg tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc
    11401 agaaattgcc tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc
    11461 tctgcgctat ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt
    11521 cgggtacgac ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta
    11581 aaaagcatgc taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa
    11641 gcatagatcc tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc
    11701 agtgaacctt gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat
    11761 gctacgttta cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt
    11821 gaactctcaa cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat
    11881 tatctgttgt ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctggcaacc
    11941 ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc
    12001 aagcagcaat ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta
    12061 ttgtctaact ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc
    12121 agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg
    12181 cgaattgtcg ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac
    12241 gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg
    12301 ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg
    12361 tcgtacttta tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc
    12421 aaacagcgag aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa
    12481 atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc
    12541 gttgctctat ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga
    12601 attatacggt caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac
    12661 gaccgcgtct gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg
    12721 cgtctgaatc aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg
    12781 aaaattgtcc gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga
    12841 tactcatcct atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca
    12901 gattgtcctg aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt
    12961 tgatttaatc attatggata tagacttgcc cggaacagac ggttttacct tcctg
//
LOCUS       DAEEGA010000100        12964 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEEGA010000100 DAEEGA010000000
VERSION     DAEEGA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12964
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.100"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..76
                     /locus_tag="KLH46_003318"
     CDS             <1..76
                     /locus_tag="KLH46_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0379894.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            110..1018
                     /locus_tag="KLH46_003319"
     CDS             110..1018
                     /locus_tag="KLH46_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379895.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVEGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
     gene            1257..1316
                     /locus_tag="KLH46_003320"
     CDS             1257..1316
                     /locus_tag="KLH46_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBE0379896.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1633..1692
                     /gene="ibsE"
                     /locus_tag="KLH46_003321"
     CDS             1633..1692
                     /gene="ibsE"
                     /locus_tag="KLH46_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HBE0379897.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1812..3245)
                     /gene="hldE"
                     /locus_tag="KLH46_003322"
     CDS             complement(1812..3245)
                     /gene="hldE"
                     /locus_tag="KLH46_003322"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBE0379898.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3293..6133)
                     /gene="glnE"
                     /locus_tag="KLH46_003323"
     CDS             complement(3293..6133)
                     /gene="glnE"
                     /locus_tag="KLH46_003323"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBE0379899.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6156..7457)
                     /gene="ygiF"
                     /locus_tag="KLH46_003324"
     CDS             complement(6156..7457)
                     /gene="ygiF"
                     /locus_tag="KLH46_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBE0379900.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEINGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7699..8319
                     /locus_tag="KLH46_003325"
     CDS             7699..8319
                     /locus_tag="KLH46_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBE0379901.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8383..9621
                     /gene="cca"
                     /locus_tag="KLH46_003326"
     CDS             8383..9621
                     /gene="cca"
                     /locus_tag="KLH46_003326"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBE0379902.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9784..10605)
                     /gene="bacA"
                     /locus_tag="KLH46_003327"
     CDS             complement(9784..10605)
                     /gene="bacA"
                     /locus_tag="KLH46_003327"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBE0379903.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10696..11064)
                     /gene="folB"
                     /locus_tag="KLH46_003328"
     CDS             complement(10696..11064)
                     /gene="folB"
                     /locus_tag="KLH46_003328"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBE0379904.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11169..11786
                     /gene="plsY"
                     /locus_tag="KLH46_003329"
     CDS             11169..11786
                     /gene="plsY"
                     /locus_tag="KLH46_003329"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBE0379905.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11799..12730)
                     /gene="ttdR"
                     /locus_tag="KLH46_003330"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11799..12730)
                     /gene="ttdR"
                     /locus_tag="KLH46_003330"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
BASE COUNT         3132 a         3642 c         3228 g         2962 t
ORIGIN      
        1 actgtcgttc tcaaaatcgg tggagttgca tgacaaggtc atcgggcatt atctgaacat
       61 aaaacactat cagtaagttg gagtcattac cgaacaacaa gaagcgttta tgacgcttga
      121 gcaggagcag caggttaaaa cccgtactgc cgaacagaat gcacgtattg cggcttttga
      181 agctgaacgt cgtcgtgaag cagagcagac gcgaattctg gctgaacgac aaattcagga
      241 aacggagatc gagcgcgagc aggccgtccg ctcaagaaag gttgaggctg aacgtgaagt
      301 tcgtattaaa gagatcgaac agcagcaggt caccgaaatc gccaaccaga cgaaatcgat
      361 cgctattgcc gccaaatcgg aacaacagtc ccaggcagaa gcgcgtgcta accttgcact
      421 cgcagaagca gtaagtgcgc aacaaaacgt agaaaccact cgccagaccg cagaagccga
      481 tcgtgctaaa caagttgccc taatcgctgc cgcgcaggat gcagaaacca aagcggttga
      541 actgaccgtg cgggcgaaag cagaaaaaga agccgcagag atgcaggcgg cggctatcgt
      601 tgagttagcc gaagctacac gtaaaaaggg tctggcggaa gcagaagcac aacgtgcgct
      661 gaacgatgct atcaacgtac tttctgatga acaaaccagc cttaaattca aactggcctt
      721 gttgcaggcg ctacctgcgg taatagaaaa atccgttgag ccgatgaaat ctattgacgg
      781 tatcaagatt attcaggtcg atggtctgaa tagtggcagc gctgcgggtg atgcaaacac
      841 gggtaatgtg gagggcggaa acctagcgga acaagcatta tcagccgctc tctcttaccg
      901 cacacaagct ccgctgattg actccttgct caatgaaatt ggcgtttcag gcggttcact
      961 ggcggcattg acttcaccct taatctcaac aactcccgtc gccgaaaacg taaaataaat
     1021 tttcagctat gaacgtccct ggcacaaaaa gccggggatt ttttatatct gcgttccgct
     1081 aaaaactgca aatgatcagg tcgttgcaac gttttgcgtg accgctcggg gaaggcaaaa
     1141 ctgcctctgg gaaagcgttg cgcgaggtcc ggcgctcatc aacaatcggg gagtcagcaa
     1201 agggctgaaa cgggaaagcc cctcccgagg aaggggcctt acagaaggaa agggttatga
     1261 tgaagctcgt catcatactg attgtgttgt tactcgtaag tttcgcagct tattaacagc
     1321 caatcagagg ggggagaaat cctccctcac ccttgttcct ttactttagg ttgaaaaaaa
     1381 atcagcgtca agaaggtttc attaacacat atccctggcc caaaaagccg gggatttttt
     1441 atatctgcgt tccgctaaaa ggtgcaaatg ctcaggacgt tgcagcgttt tgcgtgaccg
     1501 ctcggggaag gcaaaactgc ttcagggaaa gcgttgcgcg gggtccggcg ctcatcaaca
     1561 atcggggagt cagcaagggg ctgaaacggg aaagcccctc ccgaggaagg ggccttatag
     1621 aaggaaaggg ttatgatgaa gttcgtcatc atactggttg tgttgttact gttaagtttc
     1681 ccgacttact aacaactgat cagagggggg agaaatcctc ccttaccctt gttcctttac
     1741 tttaggttga aaaaacaaca gcgtcaatag gcctgcctgc tacgaagcga gatctgtgaa
     1801 ccgctttccg gttagccttt tttatcctgt tggatcttct tgatgatgtt agtggtcgag
     1861 caaccgtctt cgaagttgag caccagcact tcgccgccgt tggcccagac ttctttactc
     1921 ccggcgatct cttctggttt atagtcgccg cccttcacca gtaaatctgg caggatcccg
     1981 gcgatcaagc gctgcggcgt gtcttcttca aacgacacca cccagtcgac cgcttccagt
     2041 gcgcccagca caatcatacg ctgttcgagc gggttaacgg ggcgagaatc ccctttcagc
     2101 cgtttggtgg aggcatcgct gttcacagcg acaatcaagc ggtcacccag tttgcgggca
     2161 tttgccagat agaagacgtg tccggcgtgc aggatgtcaa agacgccatt ggtcatcacc
     2221 actttttcac cgcgtttacg cgctgccgct acggccagct tcagttcctc ttcggtcatc
     2281 acgccaaagc cggtatctgc gcgcccacgc actgcatttt ccagctcgat cggcgaaacc
     2341 gtggaggttc ccagtttgcc gaccaccacg ccagccgccg cattggcaaa gaagcaggct
     2401 tcttccagcg aattacccgc tgccagcgtt gccgccagga cgccaatcac cgtgtcgccc
     2461 gcaccggtaa cgtcatacac ttcctgcgcc tgggttggca tatgcagcgg cgctttaccc
     2521 ggttgcagca gcgacatacc ctgttcggaa cgggtcacta acagagccga gagttcgtaa
     2581 tcggcaatca gtttcatgcc gcgctcaaca atctcttctt cggtcttaca tttaccgaca
     2641 acagcttcaa attccgagag attcggtgtc agcagcgtag cgccgcggta gcgctcaaaa
     2701 tcggtacctt ttggatcaat cagcaccgga acgcccgctt tacgcgccag ttggatcatc
     2761 tgctgtacgc ttgccagcgc acctttggcg tagtcagaaa gcaccagcgc gccaatcgaa
     2821 ctcagcgcct gattaatccg ctcgtgcagc ggctgcggat caacaccttc gaaaccttct
     2881 tcaaaatcca gacggatcag ctgttggttg cgggaaagta cccgtaattt ggtgatggtc
     2941 ggatgcgtcg gtacagaaac gaagtcgcat ttgacgttga cgtcggccag agatttactc
     3001 agcgcgcgcg ctgcatcgtc aatgcccgtc aacccgacca ggcgtgcatt agcaccgaga
     3061 gaagcgatat tcatcgccac gttagccgcg ccgcccggac gttcttcgat ggtattcact
     3121 ttaaccacgg gcaccggcgc ttccggcgag atacgactgg tggggccgta ccagtaacga
     3181 tccagcatca catcaccaac caccatcact cctgcacgtt caaactctgg cagcgttact
     3241 ttcattcctg tctcctgaga gattcaaaat ttgcgcgcga taataccata cttcattctt
     3301 ccaccagcca cttctgccag cttgcccgca ccagatcacg ctctgcggtg aagcaatcct
     3361 ccgacacatg gcccggcaat tcctgtaatg ccagatgatg aagttcatcg cgcagcgtcg
     3421 tgtaagcacg ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt tgcgccagca
     3481 gctccagaat acgcacgttg tctgaccagc gcgttaactt cggtttttca tgagcgtagc
     3541 gcaacaccag atattgggta ataaattcga tatcggtaat tccgccttca tcggctttga
     3601 tatcaaagcg attacgatgt ttattgccga gatgagcgcg cattttctcg cgcatttccc
     3661 gcacttccgt ttgcagagtt ttaccttctc gcggcagtgt cataatctca cgacgcaccg
     3721 cgtcaaagtg cgcagtgagc tgcggatcgc cgtacactac acgcgcacgc accagcgcct
     3781 gatgttccca cgtccaggcc tcgtttttct gataatcggc aaatgcttct gcggatgtca
     3841 ccagcattcc cgccgccccg gacggacgca gtcgagcatc cacttcatac aaaatgccgg
     3901 aagaggtacg cgtactgaac agatgcataa tgcgttgcgc cagacgcaga taaaactgcc
     3961 gcccgtcgat ttcccgctcg ccgtcagtca tcgcatccat tgggcaatca tggaggaaga
     4021 taaggtcaag atcggaactg tagcctaact cccagccgcc cagcttgccg tagccgacca
     4081 ccgcaaaacc gcgcccttca cgttcgttga ggtgatttgg cttaccgtag cgggccacca
     4141 tttgacccca cgcctgctga acgacggcat ctatcatagc ttctgccagc caggttaagt
     4201 gatcgctcac tttcattacc ggtagcgtac cggcgatatc cgctgccgca atgcgtaaca
     4261 gctgcgcctg tttgaactga cgcagcgcct caagctgttg ttcttcgtca tcttccggca
     4321 cgcgcagcaa atactggcgc agttcatcac ggtaggcatc ggtcgccgtc ggctgataaa
     4381 gggtatttgg atcgagcaat tcatccagca ataatggata acgcgctagc tggctggcaa
     4441 tcatcggcga tgcggcacac agagaaatca aatgtttaag cgccgcaggg aattcactga
     4501 gcaattctaa ataggttgtg cgggtaacaa tccccaccag caaggcggta atgcgcgaca
     4561 gcgtgacagc agcgtcttca cgcgcacaga catcacttag cagatgtggc atcagatggt
     4621 cgagcacctg acgtcctcgc ggcccgatag tacgcttatc cagctctttg cggaaatcag
     4681 caatcagcgt taggacctgt ttgcgatcat cctcgctaag atgcgccagc actggcgtag
     4741 tgtcatcttc ctgcaacgca tcctgccaca gctcacgcca ctgttccgac agcgactctt
     4801 cctgagtttc actttcgtcg tcgccaatca attcattaaa cacccggcgc acattggtca
     4861 tatgtgcggt cagcgccccg gtcagttgcg gccagtcagc aaagtccatc gcccacgcca
     4921 gccgcgcacg gttaagctca tcagaaggaa gcgtttgggt ttgttcgtcg ttaatgcttt
     4981 gcagcaggtt ttccagacgc cgcaggaaca gatatgccac tcgcaattgt tcagcatcgt
     5041 tttcagaaag cagatgcagc gcggcaatgg cgctgagcgt tggcagtaaa gagcgcgact
     5101 gcagcaacgg ttcgcgccca ccgcgaatga gctggaacac ctgaacgata aattcaattt
     5161 cgcgaatgcc gcctgcgccg agtttgatat tgtcggtcaa accacgtcga cgcacttcac
     5221 gggcaatcat ccctttcatg ttgcgcagcg actgaatcac gctgaaatcg atgtaacgac
     5281 ggaaaacaaa cgggcgcagc atcgcacgca actcgttagc atagacgcct tcgctatcgc
     5341 ccataatccg cgctttgacc atcgcgtaac gctcccagtc gcgtccctgc tcctggtaat
     5401 aatcctccaa cgcggcaaag ctcagcacca gcgggccact ttcgccaaac ggacgcagtc
     5461 gcatatccac gcgatagacg aagccatcct gcgttggttg atccagcact ttaatcagcc
     5521 gctgccccat gcgggtaaaa aactgcgcgt tatccagttc ccggcgtccg ccctgcgtac
     5581 aaccatgttc cggccaggca aaaatcagat cgatatcaga ggagaaattc agttccccgc
     5641 cgcccagctt acccatgcct aagatcagca gcggttgcgc ttcgccctgc gcattgcacg
     5701 gcgttcccca ctcgcggcag caggcgtcat acagccagtc acgcgccgca acaatcagcg
     5761 tctccgccag ataactgagc tgctgcaata tgctctcttc agtaaccagt gccagcgttt
     5821 gcgcccatgc gatgcgcacc ataatgcgcc gccggaatag ccgcagctcg cgcattaacc
     5881 cggcttcgtc gctcacatta ctgagcgcct cctgcaacca cacgctgtaa tgttgccatt
     5941 cgtcagcctg tggcggttgg ctttccagtt ccgtcagcca ctctggatgc gcaatcacgc
     6001 tgtcctgcac aaaatcactg aaagtaagta ctgacttcgc ctgtgcacta agtgattcct
     6061 cggctaaaag ctctggcagc cgctcaacaa cggtctgcca gtactgctgt aacggtgaag
     6121 agagcggctt cataagcgat tttatccttg cctgattaac gttttccact gtgcaaccag
     6181 aacggttcct gattgtttgc ctcattacgg aaatgttcaa tttcaatgcg ttgcccggtc
     6241 gcaatagcgt gacgcagccc ctgccagttc tccagccagg cttgcgcgac gacaggatca
     6301 tagtaacccg ccagcaacag tattgagtca atatcacgca tcaggcgtgg caactggtca
     6361 cggtagcgat cgcctaacgg ctggcagaaa acgcttttca gttcagcggc atggcgggaa
     6421 agatggatat cggcaaagcg tttgaaggag tcgctaattt tgccctgcgc tttggcatct
     6481 aaaaacggct gccatgcttt gcttaccagc cattcggtca acgccagctt cgccattgcc
     6541 gtttcggtag agtagaccgc cgtcacggca gaaaccgcag aagcgatggt cgcttcgcat
     6601 tgagtcagca aatcacgtaa gtgagtactc gctttacgcg gcacaatgcc accgaacagc
     6661 atcagtgtat gacggactag gctaatggct gccagcacct gctctttcgc cgcatcgttg
     6721 ccgcgtaccc acagttcttc atgatactgc cattgcgcta acaccagctc gagcgccgct
     6781 tccagcccct gttccacatc ggcttttgcc gcaacatgca aaatggtggt cggtttgatt
     6841 tcacgcgccg gattgccctg cgccagatga tagccacgcg ccgctttgct caggctgccc
     6901 tggcgtaatc cggtttgcga taccagttgg ttcgccagtt tcagcaccgc gcgcgtgtcg
     6961 ccgctaagca gctccagttc cagctcgcag ataggctcag caaattcacc cgctttcact
     7021 tccccctggt cgagagcgat ttcaattcgg ctgccattga tctctactag ccatttttcg
     7081 cgataaaaat cggtgctaaa cagcgactgc acgcgggaag cgagatcggc aggcaattcg
     7141 ccgttcggcc agatttccgt cggtaactgc gccaggtcga gcgtcggttc gctcaacgcc
     7201 acgttatatt ccgggcgctg gtgtaagccg cctgtcactc ttcctgcaac tttcatggtc
     7261 atctcatagc gaccgttttc gccacgaata cgtaagccca tatcgtgcct acgcagccag
     7321 ttatccggcg tttcgtagta aatattcagc aactgcacgg ggtcatggtg ctcgccgccc
     7381 agcgtattga gatggtcacg caacgcctca acggcactgt gattaacaat aaactttaat
     7441 tcgatttcct gagccatggc ctttacttat cggttatgtc acataagtga cgatgaacgg
     7501 cgaacttaat gcgatctttt ttgtcagtag atagtatttt gcgccaaatt gccatgcaac
     7561 gggcaatttg acgggcgtaa aagtttgaag cagtggcaag gcctgacaca gaggattccg
     7621 attgatgtcg aatgctttgc gtagcgacac tgatgccact actatcgttc cactttccat
     7681 gacaataacg acagcctgat gccaaaatta cgcctgatcg gattaacttt actcgcactt
     7741 agcgcgactg ccgtctcaca cgctgaagaa acgcgctatg tttctgacga actgaatacc
     7801 tgggtccgta gcggtccggg agatcattat cgcctcgtgg gcacggttaa cgccggcgag
     7861 gaagtgacct tattacaaac tgacgccaac accaattatg cccaggtgaa agacagctct
     7921 ggccgtaccg cctggatccc gttgaaacaa cttagcactg agccaagcct gcgctcccgt
     7981 gtgccagatc tggaaaatca ggtcaaaacc ctgaccgata aactcaccaa tatcgataac
     8041 acctggaatc agcgcaccgc agaaatgcag caaaaagtgg cgcagagcga cagcgtgatc
     8101 aacgggttaa aagaagaaaa tcagaaactg aaaaacgagc tgattgtcgc gcagaaaaag
     8161 gtcgatgccg ccagcgtaca gctggatgac aaacagcgca ccatcatcat gcaatggttt
     8221 atgtatggcg gtggcgtgct ggggcttggc ttgctgctcg gtctggtact gccgcacctg
     8281 atcccaagcc gcaaacgcaa agatcgctgg atgaactaaa tcgccttctt cgttgcactg
     8341 tcttattatt aggaattgtg tatttgacga agggatgatg gcgtgaagat ttatctggtc
     8401 ggtggtgctg ttcgggatgc attgttagga ctatcggtca aagacagaga ttgggtggtg
     8461 gtcggcagta cgccacagga gatgctcgac gcgggctacc agcaggtagg ccgcgatttt
     8521 cctgtgtttc tgcatccgca aacgcatgaa gagtatgcgc tggcacgtac cgaacggaaa
     8581 tccggttccg gttacaccgg ttttacttgc tatgccgcac cggatgtcac gctggaagat
     8641 gatcttaagc gtcgcgatct gaccattaat gcgctggccc aggacgataa cggcgagatt
     8701 atcgacccgt acaacggtct gggcgatctg caaaatcgtc tgttgcgcca tgtttccccc
     8761 gcttttggcg aagatccgtt acgcgtactg cgcgtggcgc gttttgctgc gcgttatgcc
     8821 cacctcggtt ttcgtattgc cgatgaaact ctgacgctga tgcgcgagat gacccatgcg
     8881 ggtgaactgg aacacctgac gcctgaacgg gtatggaaag agacggaaag cgccctcacc
     8941 acccgcaatc cccaggtgtt cttccaggta ctgcgcgatt gcggcgcact tcgcgtttta
     9001 ttcccggaaa ttgacgcact gtttggcgtt ccggcacctg ccaggtggca tccggaaatc
     9061 gacacgggta ttcatacctt aatgacgctc tcaatggcgg cgatgctgag tccgcaggtt
     9121 gatgtccgtt tcgcaacttt atgccacgat ctcggtaaag gactgacgcc gccagaactt
     9181 tggccgcgtc atcatgggca tggcccggcg ggtgttaagt tagtggaaca actatgccag
     9241 cgtctgcgcg tgccaaatga aattcgcgat ttagccagac tggtggctga gtttcacgat
     9301 ctcatccgca ccttcccaat gctgaaccca aaaaccatcg tcaaattatt tgattccatc
     9361 gatgcctggc gtaaaccgca gcgtgtcgag caactggcgc tgaccagcga ggctgacgtg
     9421 cgcggcagaa ccggttttga atcagcggac tacccgcaag gccgttggtt gcgcgaaggc
     9481 tgggaagtgg cgcagtcagt gccgacaaaa gcggtcgttg aagcgggatt taaaggcgtg
     9541 gagattcgcg aggagctgac ccgacggcgg attgcggcgg tagccagctg gaaggaacaa
     9601 cgttgcccaa agcctgactg agtataaaac taaacgttag cacgaaccgt cccctcgccc
     9661 cttcggggag agggttaggg tgaggggaac agaccagcac cggtgcgaac aatcaccccc
     9721 accccggccc tctccctaca gagcgaggga gaaaacccgg cctgctcccg aaccttttct
     9781 caatcaaaag aacacgacat acaccgcagc cgccacaata aagcgataaa tcgcgaacgg
     9841 gataaacgaa atgcgcttaa tcaattgcag gaaggtttta atcgctatca gcgccaccac
     9901 aaaagcggtg ataaacccaa cggcaaacat cgggatatcg ccggttgtca ggaagcccca
     9961 gcttttgtag agatcgagcg ccgttgcgcc catcatcatc ggtaccgcca gcaggaacga
    10021 gaactcggaa gcagcgtaac ggctcacgcc catcagcatc ccacctgaaa tggtcgcccc
    10081 ggaacgggag aaacccggcc acagcgccag acactggaaa cagccaatca taaacgcctg
    10141 acgataggtc atatcatcaa gacccggcgc acgcggctct ttcggcttca ggcattcggc
    10201 ggcaatcagc aacaaaccgc caacgaccag cgcatacatc acatttatcg ggttaaacaa
    10261 tgacttaatc gtgtcgtgga ataatagccc cagtaccacc gccggaatca tccccagcaa
    10321 aatgtggatc agcgttaaac gacctttgct ttcaccttcg tgctgcaacg ggcggccaaa
    10381 gtggatgcca atcaggccaa acagacgccg ccagaacatc actactaccg ccagaattga
    10441 tcctaactgg atcacaactt caaaggtttt cgccgtgtcg ccctcaaacc ccaacaagtg
    10501 accgacaata atcatatggc ccgtgctgga taccggcaga aattctgtca atccttcgac
    10561 cacacccaat attgccgcta tcagcagcga gtgcatatcg ctcatcaata aacccctaaa
    10621 ttattaaaat gtaccgcttg tccgaactac tgcagtatga ccaggttata accgtttggt
    10681 ttaacagctg taaaattaat tattttcttt cagattattg ccacgctcaa tgattacgcc
    10741 aacattcgcc gcccgcgcca ctgcgcctgg cttgctgagt ttgatacgca cccacggcga
    10801 gttgaagcgt gctaacagca gctccgccac ctcttcagcc acgcgttcca ccagcgcaaa
    10861 acgcgccccc tcgacgtggc tgaccaccgt ttctgcaatg tcagcgtaac tgaggcaatc
    10921 cgccacatca tcacttttcg ccgctttacg gttatcccat gccatttcga tatcgaacac
    10981 taacttctgt tcgatggtct gttcccagtc gtaaacacca atagtggtga ttaccgaaag
    11041 ttgctctata aatacaatat ccatcacgtc ctgcctgctt tttggctaac cggataccac
    11101 ttccggcgaa atgtgcgtat tatccacaga ttcatcgttg aacacgaatt ttcaaaacgg
    11161 aacagcttat gagtgcaatc gcgcctggaa tgatcctcat cgcgtacctc tgcggctcca
    11221 tttccagtgc cattctggtt tgccgcttgt gtgggctgcc cgatccgcga accagcggct
    11281 ccggcaatcc aggcgcaacc aatgtgttac gtatcggtgg caagggagca gccgtagcag
    11341 tactgatttt cgacgttctg aaaggaatgt tgcctgtctg gggcgcgtat gaattaggtg
    11401 tcagcccctt ctggctaggc ttaattgcca tcgccgcctg tcttggacac atctggcccg
    11461 ttttcttcgg ttttaaagga ggaaaaggcg ttgctaccgc ttttggagca atcgcaccaa
    11521 ttagctggga tctcaccgga gtaatggcgg gaacctggtt actgaccgtg ctattgagcg
    11581 gatactcgtc gctgggagcg attgtcagtg cactgattgc tccgttttat gtctggtggt
    11641 ttaagccaca attcaccttc ccggtttcga tgctctcttg cctgatcctg ctgcgtcatc
    11701 atgacaacat ccagcgtctg tggcgtcgtc aggagacaaa aatctggacg aaattcaaaa
    11761 gaaagcgcga aaaggatccc gagtgatttc tggtggatct acatgacctg atagccttca
    11821 tcgggcttgc ccagccgttg ctggcaccat gccgccagaa attccgcgca gacacgaatt
    11881 tcatgctgcg atagagcagc tcccggtaaa cagcccagat attggcgctt tgtgcatact
    11941 ccggcaatac ttgcaccagt ttgccactct ccagaaacgg caacacatcc cactcggaac
    12001 gcagcataat ccctttgccc tccagcgccc attgcaacac tatctcgccg ctattggagg
    12061 aaagatgccc gctaaccttc accgattttt tctcctgacc gttccccaac tcccatattc
    12121 cgtgggtcat atcgcgctct ttggtcacca gacagtcatg acgacttaat tcctgtaatg
    12181 attgtggctg cggatatttt tgcagatact caggtgctgc gcataatatt cttttatttt
    12241 tcgttaacag atgcgcaata taataatcag gaatttcgtc attaatacga atatccagat
    12301 caatattatc ctgcactaaa tcaatttgcc gatcgaacaa ttcaaaatgc acctgtaact
    12361 caggataatt gcgcataagt tcggtaatgg ctggcgcaat atggctgcgc ccaaaaccaa
    12421 aactgcaacc aatacgaatc atcccctccg ggcgcgtttt gatttgcgtg acgtcatcga
    12481 ccagtcgctg atattgggtg aggatttcca gcgcatgttc gtaacaacgt tggccacttt
    12541 cagtcagcgc cacgccgcga gcggagcggt tcagcagcgt tgttgccagc gtattttcaa
    12601 ggatttggat ccgcttggtg acaaatgcag gcgtttgccc aagcgtcgcc gctgcagcac
    12661 taaaactccc gctgtggacg atttctacca gtacctgcaa gtctttggct aaaggccagc
    12721 tattcagcat cgttaattat ccgcagttgt gataagcgca gtgtatttcg caaaacattg
    12781 ccattccctg ctctggcgca atattcattc ttattttcgc gttgattcac gaactgttaa
    12841 ttacatattc acggtggcaa aaaatataaa accacatttt tagtgttagt ttgtggcggt
    12901 gaatttttcc agacaaatac aaaaactgga gttgccatga tgagcgaaag taataagcaa
    12961 cagg
//
LOCUS       DAEEGA010000101        12805 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEEGA010000101 DAEEGA010000000
VERSION     DAEEGA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12805
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.101"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..351)
                     /locus_tag="KLH46_003331"
     CDS             complement(<1..351)
                     /locus_tag="KLH46_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0379906.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKI"
     gene            727..2367
                     /gene="trg"
                     /locus_tag="KLH46_003332"
     CDS             727..2367
                     /gene="trg"
                     /locus_tag="KLH46_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBE0379907.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2405..3328)
                     /locus_tag="KLH46_003333"
     CDS             complement(2405..3328)
                     /locus_tag="KLH46_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0379908.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3545..4888
                     /locus_tag="KLH46_003334"
     CDS             3545..4888
                     /locus_tag="KLH46_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE0379909.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMTPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            5113..6768
                     /gene="opgD"
                     /locus_tag="KLH46_003335"
                     /gene_synonym="mdoD"
     CDS             5113..6768
                     /gene="opgD"
                     /locus_tag="KLH46_003335"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBE0379910.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6908..7132
                     /locus_tag="KLH46_003336"
     CDS             6908..7132
                     /locus_tag="KLH46_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBE0379911.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            7195..7731
                     /gene="rimL"
                     /locus_tag="KLH46_003337"
     CDS             7195..7731
                     /gene="rimL"
                     /locus_tag="KLH46_003337"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBE0379912.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7726..8706)
                     /locus_tag="KLH46_003338"
     CDS             complement(7726..8706)
                     /locus_tag="KLH46_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379913.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8830..9822
                     /gene="tehA"
                     /locus_tag="KLH46_003339"
     CDS             8830..9822
                     /gene="tehA"
                     /locus_tag="KLH46_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBE0379914.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9819..10412
                     /gene="tehB"
                     /locus_tag="KLH46_003340"
     CDS             9819..10412
                     /gene="tehB"
                     /locus_tag="KLH46_003340"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBE0379915.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10715..11383
                     /locus_tag="KLH46_003341"
     CDS             10715..11383
                     /locus_tag="KLH46_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBE0379916.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11401..11907)
                     /gene="tnpA"
                     /locus_tag="KLH46_003342"
                     /pseudo
     CDS             complement(11401..11907)
                     /gene="tnpA"
                     /locus_tag="KLH46_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11915..>12805
                     /locus_tag="KLH46_003343"
     CDS             11915..>12805
                     /locus_tag="KLH46_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0379917.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNR"
BASE COUNT         3338 a         3005 c         3201 g         3261 t
ORIGIN      
        1 aatcttgagc gcgcgtaaac cagcttctcc gcgctcttca tagaccttca gccacctggc
       61 tacagaacca ctaccagcaa gcataaagtg agcagcagcc tgattaaggg acatgtgctg
      121 ctcgatcaca gctttcacga ccttaatacg caactctgga tcagcactaa cgcctttagg
      181 tttgggaatt aaaccttttt ctccatgttt ttcatagagg gcaacccatg tcctgacctg
      241 ggttcggggg acaccaaaac gtgccgagat gatcctgtaa ccatcatcag tagtgaagta
      301 gtgattcacg acttcaaggc gcttttcaaa agggtatttt ggctttgaca tattaggggc
      361 tattccattt catcgtccaa caaaatgggt gcagtacagt tgatagaaat atcggtcggg
      421 gccttcgtct ttctgattcc cggttagcct gaaaacagaa agtctcaggc acccgcaggc
      481 atcctatgag gtttccttag ggacgaaaat aatcacttca cgaaattgcg tgctgttttc
      541 cagaattttt cgtcattcgg gttagccagt ttagccattc gttactctct tcattccaat
      601 agcattaatt ttctctgcaa taattgttgt aaaaatgtga cgcaaagagg tttttggtca
      661 taagtaatta ccgtcaagtg ccgatgactt tctatcagga gtaaacctgg acgagagaca
      721 acggtaatga atacaactcc ctcacagcga ttaggttttt tgcatcacat caggctggtt
      781 ccgttatttg cctgcattct tggcggtatc ttagttctat tcgcattaag ttctgctctg
      841 gctggctatt tcctctggca ggccgatcgc gatcagcgtg atgttactgc ggagattgag
      901 attcggaccg ggttagcgaa cagttcagat tttttgcgtt cagcccggat caatatgatt
      961 caggccgggg ctgcgagtcg tattgcggaa atggaagcaa tgaagcgaaa tattgcgcaa
     1021 gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg cttatcagaa tcgaccggtg
     1081 aaaacacctg ctgatgaagc cttcgacact gaattaaatc aacgctttca ggcttatatc
     1141 acgggtatgc aacctatgtt gaaatatgcc aaaaatggca tgtttgaagc gattatcaat
     1201 catgaaagtg agcagatccg accgctggat aatgcttata ccgatatttt gaacaaagcc
     1261 gttaagatac gtagcaccag agccaaccaa ctggcggaac tggcccatca gcgcacccgc
     1321 ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc tggtcatgac gctgataaca
     1381 tttatggtgc tacgtcggat cgtcattcgt ccactgcaac atgccgcaca acggattgaa
     1441 aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg cgggtcgtaa tgaaatcggt
     1501 cgcttaagtc gtcatttaca gcaaatgcag cattcactgg ggatgacagt agggactgtt
     1561 cgacagggcg cggaagagat ttatcgtggc accagcgaaa tttcagctgg caatgcggac
     1621 ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac aaactgccgc cagcatggag
     1681 caactcactg cgacggtgaa acagaatgcg gataacgcgc atcatgccag caaactggcg
     1741 caagaggctt ctattaaagc cagcgatggc gggcagacgg tttccggtgt agtaaaaacg
     1801 atgggcgcta tctccacgag ttcgaagaaa atttctgaga tcaccgccgt catcaacagt
     1861 attgctttcc agacgaatat tctggcactg aatgctgccg ttgaagccgc gcgagcgggt
     1921 gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac ggacactcgc aagccgcagc
     1981 gctcaggcag cgaaagagat tgaaggcttg atcagtgaat cagtcaggtt aattgacctg
     2041 gggtcggatg aggtggcaac ggcagggaaa accatgagca ctattgttga tgccgtcgcg
     2101 agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg atgaacaaag tagaggcata
     2161 actcaggtta gccaggcgat ttctgaaatg gataaggtga cgcaacagaa tgcttctctg
     2221 gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac aggctgcacg attaactgag
     2281 gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg cagaacctcg cggagcgggt
     2341 gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga gggatcgaca gatcccttca
     2401 cctttcagaa cggcattgat tttcgaatag cgttaatcat caactggcaa ccagaagaga
     2461 acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc tgcaccatgt cccggaacag
     2521 gcagggccac cagcgtggca tgacgcaggt cgtcttttac agcgccagaa gggacaaacc
     2581 acacgtaatc gtattcaacg gtaagttgac gagatagcga agtagacagc gtttcgatac
     2641 aacccgaagg aattttacag ccctggctct gcactaatgc atctgaatgc tggcgtggcg
     2701 cagtgccttc tggtgataca acgaccggcc attccagcac ccggcttagc gttacgttct
     2761 cctgaagtag cgggtgatta gggcggacaa ccagcttcaa cgattcaaga aacagcagtt
     2821 cgtaattaag cccggtcatc agttcaggat ctgacatccg accaatgccg atatcgattt
     2881 ccccggtttt taaacccgcc agaatcatag ggttactcat tgtcgcaact tgcaaggtcg
     2941 tctctttttg ttgctgatga aactgaccta taaccgaagg taatatcccc agtgccgcag
     3001 taggtagtgc accaaccctg acgacatcat tattaagacc ttctttacga tgaagcgact
     3061 gtccggcagt gttgatggcg tcaagaactc tgactgcatg cgttaaaaat tgttcgccgg
     3121 gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa gcgagcgccc gtcagctgct
     3181 ccagttcatt caatgtatta gagagcgcag gttgactcaa attaagggtt tcagctgcgc
     3241 gccccaaagt tccttgttgt gcgacagcta cgaatgtatg aaggtggcgc aaacggatgc
     3301 gctgactaaa cagactattt ttttccataa gcgatgttaa aaacgaagcg gtgtcgctga
     3361 caagtgaagt tgtttgatta tgataacttg attgcaaaat attattaata attaaagcaa
     3421 ttatgttaca gcaaaatgga taatattgat gttttcgcgg cgagatcaca gtttgtaaat
     3481 tcttcccgcc agagtgaatg cggttaccta cactccagat tactgaccac tggaggcaga
     3541 cactatggcg aacagcatca cggcggatga gattcgggaa cagttttcgc aggcaatgtc
     3601 agccatgtac cagcaagaag ttccgcaata tggcacgctg ctggaactgg tagctgatgt
     3661 gaatctggct gtgctggaaa acaatcctca actgcacgaa aaaatggtaa atgcagacga
     3721 gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc gttgggactg cacaagagct
     3781 tgctactctt cggcggatgt ttgccattat ggggatgtac ccggtgagct attacgatct
     3841 ctcgcaggca ggggtgccgg tacattcgac agcatttcgg cccattgatg atgcttctct
     3901 ggcgcgtaat cccttccgcg tttttacatc cttactccgc cttgagctta tcgagaacga
     3961 aattttgcgc cagaaagcgg cggagattct gcgtcagcgc gatatcttca ccccacgttg
     4021 tcgacaactg ttagaggaat atgagcagca gggcggtttt aacgaaacac aggcacagga
     4081 gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag ttagcaacgg tagatgaaga
     4141 aacctatcgc gccttgcaca acgaacatcg gttgattgct gatgtggtct gttttcctgg
     4201 atgccatatc aaccacctga cgccacgtac gctggatatt gaccgggtgc agtcgatgac
     4261 gcctgaatgc ggaattgaac ccaaaattct catcgagggg ccgccgcgcc gcgaggtacc
     4321 gattttacta cgccagacca gctttaaagc actggaagag acggtgttgt ttgcggggca
     4381 gaaacagggc acgcataccg cgcgctttgg tgaaattgag cagcgtggcg tggcattaac
     4441 gccgaaaggg cgacaactgt atgatgatct tctgcgtaac gctggaaccg ggcaggataa
     4501 tctcactcac caaatgcatt tacaggaaac cttccgcact tttcctgaca gtgagttttt
     4561 aatgcgtcag caagggttgg catggttccg gtaccgtctg acgccttcgg gtgaggcgca
     4621 tcgtcaggcg attcatcctg gagacgatcc acagccctta attgaacgtg gttgggtagt
     4681 ggcgcaaccc atcacctatg aagatttctt gcccgttagc gcggcgggga tcttccagtc
     4741 aaatctgggt aatgaaacgc agacacgcag tcacggtaat gccagtcgcg aagcatttga
     4801 gcaggcgttg ggttgtccgg ttttggatga gttccagctt taccaggaag cggaagaacg
     4861 cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc cgcctttgca aaaatttgcc
     4921 tgattttaca aacgaatcag gctcatccca tcgacataaa aaaaatgccg atttatgcat
     4981 attctctcag ttcaacaatt ggattattaa taaatattgt ctagagtgag cggtcataaa
     5041 taagcacttt cttgccgctg aaaacgacca gcgcgggacc attcacaaca ccagaaggac
     5101 tcactttcag gtatggatcg tagacgattt attaaaggtt taatggctat ggccgccgtg
     5161 tgcggtacca gcggcattgc ttctcttttt tctcaggcag cattcgcggc agattctgat
     5221 attgccgacg ggcaaaccca gcgttttgac ttctccattc tacagtcaat ggcgcacgac
     5281 ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac ctgacacgct ggcgacaatg
     5341 acgccgcagg cttataacag tattcaatac gacgccgaaa aatcgctctg gcataacgtt
     5401 gagaaccgtc aactggacgc tcagttcttc catatgggaa tgggattccg tcgccgcgtt
     5461 cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg aaattcactt tcgcccggag
     5521 ttgttcaaat acaacgatgc aggtgttgat accaaacaat tagaagggca aagcgatctc
     5581 ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg cgcgccgtga tgtagtatca
     5641 tttctcggcg cgagttattt ccgcgccgtt gatgatacat atcaatacgg tttgtcggcc
     5701 cgcggcctgg cgatcgacac ttacaccgac agtaaagaag agttccccga ctttaccgcc
     5761 ttctggtttg atacggtaaa accgggggca actaccttta ccgtttatgc gttgctcgat
     5821 agcgccagca ttactggtgc ctataagttc actatccatt gtgagaaaag tcaggtgatt
     5881 atggatgtgg aaaatcacct gtatgcgcgc aaagacatta aacagctggg catttcgccg
     5941 atgaccagta tgttcagctg cggtactaat gaacgtcgga tgtgcgatac aattcatccg
     6001 caaattcatg actctgatcg tctgtccatg tggcggggca acggcgagtg gatttgccgt
     6061 ccgctgaata atccgcaaaa attgcagttc aatgcttaca ccgacaacaa cccgaaaggg
     6121 tttggtttat tgcaactgga tcgtgacttc tcccattatc aggacattat gggctggtat
     6181 aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt ggggtaaggg caccatcggc
     6241 ctgatggaaa tcccaacaac gggcgaaacg ctggataaca ttgtctgctt ctggcagcca
     6301 gaaaaagctg taaaagcagg tgatgagttt gcattccagt atcgtctgta ctggagtgcg
     6361 caaccgcctg ttcattgccc attagcgcgc gttatggcga cgcgtaccgg catgggcggt
     6421 ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa aatgggcgcg tcgttttgcc
     6481 gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa aaggcattga gccggtgatt
     6541 acgctttcca gtggggaagc gaagcaaatc gaaattctct atattgaacc catcgatggt
     6601 tatcgtattc agtttgactg gtatccgact tcggactcca ctgatccggt cgatatgcgg
     6661 atgtatctac gttgtcaggg ggacgctatc agtgaaacat ggctgtatca gtatttcccg
     6721 ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga tgagttaatc gttttttctt
     6781 cggcaccttc ttcgggaggt gccgtctggt taaacacgat cccgctcgca tttttcccta
     6841 agttaaatga gtaatctgat ggtgtgtatt tcagatacac cgtgtcagcc actaacaggg
     6901 agtgcgtatg tttccagaat accgagattt aatatcccgt ctgaaaaacg aaaatcctcg
     6961 ctttatgtcc ttgttcgata aacacaataa acttgatcat gaaattgcca gaaaggaagg
     7021 ttccgacggt cgagggtaca atgcggaagt ggtccgcatg aaaaaacaaa agctacagtt
     7081 aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc aaagaggtgt aaactttcct
     7141 aagccgccag ccaggcggct tttttaacaa cagcatggat tgactggaga taagatgact
     7201 gaaacgataa aagtaagcga atcacttgaa ttacatgctg ttgcggaaag tcacgtcaca
     7261 cctctttatc agttaatctg taaaaataaa acctggttac agcagtcgct aaactggccg
     7321 cagttcgttc agagtgaaga ggacacgcga aaaacggtgc agggtaatgt gatgttgcat
     7381 caacgcggct atgccaaaat gttcatgatt ttcaaagaag atgaacttat cggcgttatc
     7441 tcgtttaatc gtattgaacc actgaataaa actgctgaaa tcggctactg gctggacgaa
     7501 tctcatcagg ggcaggggat catttctcag gcgctgcagg cattgattca tcattacgct
     7561 cagtctggtg aacttagacg cttcgtgatc aaatgtcggg tggacaatcc gcaaagcaac
     7621 caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc tgaaacaggc tgagttcctg
     7681 aatgatgcct atgatgatgt gaacttatac gcgcgtatta tcgattcata ataaccctgc
     7741 cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact tttggaccac ggacatgaac
     7801 cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt aactccacat ggttttcaat
     7861 tatcaccgca cctgtaatac ggctctcacc ttgtattaca acgtgctcgt cgagcagaat
     7921 cggtccacca cgtaccacgg catttccgcc aatcaagaca tgatgtttta acacacaatt
     7981 accttccaca atggcatatt ccgccacctg cgaactgtaa tgaatcgtcg gaatggcatc
     8041 ttcttctatg ccagctttca cctgcgcgtg accgtagact ttagcgcaat cgcatagcca
     8101 gacgttgttc tcttcattac cttcaacact ggcaaaatca aaaacttcgg cgcgatgttc
     8161 aataaaagca taccggacga cggcatcgcc ataaatttgt gcctggtgga caatacgcga
     8221 ggcgctaacc cttgcgcgat cataaatttg gagcaggagt tgatggtcgg gcgttaagcc
     8281 ttgtgcggcg acgatcatag aatgttgatc aattaaggcg tgaccaaata ttctacattg
     8341 cacgtaaact aatgagtcat gaatggtgac actgtcactt atatatgcac cctggctgat
     8401 ttcactatta tcgatccaga cgttatccgt tgcgtaaacc tctccccaca gcacgctggt
     8461 gccggtgata cgcgtattac cggaaatcac cgttccgccg aacgcaatag cattctggtc
     8521 gtaaatccag caatttcctt cctgtgctaa caccgtttcg cgatcgatcc agccgccagc
     8581 ggttcctgct atcacatcgt taaaatcgct aatggcgata atctgccgta ataacacact
     8641 ttttttagtg ccatcttcct gataactaaa ggctcgctgt tcttcactta ggcgatattt
     8701 gcgcatagcg ttttcccaca ggtgacttac tataaccgta gcaaattctg cggctctggc
     8761 tatgctcgag aaaattccat aaaatgcatt tcaaatatac tttataaatt aaacaaaatg
     8821 agtaagaaga tgcagagcga taaagtgctc aatttgccgg caggctactt tggtattgtg
     8881 ttggggacga tagggatggg atttgcctgg cgctatgcca gccaggtttg gcaggtcagc
     8941 cactggttag gggatgggct ggtgattctg gcgatgatca tctggggatt attgactagc
     9001 gcatttatta cccgactcat acgctttccg catagcgtgc tggcggaagt tcgccatcca
     9061 gtgctgagca gttttgtgag tttgtttccg gcaacgacga tgctggtggc gattggtttt
     9121 gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt ttggtgtcgt ggttcagttg
     9181 gcttatgccg cctggcaaac tgcgggatta tggcgcggat ctcaccctga agaagctacc
     9241 acgcctggac tgtatctgcc gacagttgcc aacaacttta tcagcgcaat ggcctgtggt
     9301 gcgttgggct acaccgacgc cggtctggtg tttttaggcg caggcgtttt ctcatggcta
     9361 agccttgaac cggtgatctt gcagcgtctg cgcagttcgg gagaattacc cacggcactg
     9421 cggacatcac tcggcattca gctcgctcct gcgctggtgg cctgtagtgc ctggctgagc
     9481 gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt tcggttatgg actgctgcaa
     9541 ctgctgttta tgctacgtct gatgccatgg tatctctccc agccatttaa tgcttcattc
     9601 tggagtttct cgttcggcgt atctgcactg gcaaccaccg gtttgcatct ggggagtggc
     9661 agcgataatg gatttttcca tacgctggcg gtgccgctgt ttatctttac caattttatt
     9721 attgcaatac tgctcatccg tacttttgcg cttctgatgc agggaaaatt gttagtcaga
     9781 accgagcgcg ccgttttaat gaaagcagag gacaaagaat gatcattcgt gacgaaaact
     9841 attttactga taaatatgaa ttaacccgca cacactctga agtactggaa gcggtgaaag
     9901 tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa tggtcgtaac agtctttacc
     9961 tggcagccaa tggttatgat gttgacgcat gggataaaaa tgccatgagt atcgccaacg
    10021 tcgagcgcat taaatccatt gaaaatctgg ataatttaca cacccgggtc gttgatctga
    10081 ataacctcac atttgatgga cagtacgatt ttattctttc gactgtggtg ctgatgttcc
    10141 ttgaggctaa aaccatccca gggttgattg ccaatatgca acgttgcact aaacctggtg
    10201 gttacaacct gattgtggcg gcgatggata ccgctgatta tccatgtacc gtcggcttca
    10261 cgtttgcctt caaagaggga gaattacgtc gatattacga aggctgggag agggtgaaat
    10321 acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa cggtaatcgt attaaactgc
    10381 gtttcgccac gatgctggca cgtaaaaaat gacccggtaa gcacaaaacg cgtgaaaatt
    10441 ccccacgctg agatgattta ctgttcttct tttcggtaag catatttttt atcgaaggga
    10501 tgtgaaatta atcacagtag tcgaagtttt ttagcagctt aacttactga aatttaacta
    10561 ctgatgattg acttagcccc tttttcggca ttgactatgt cgtctgaaaa ggggctgaaa
    10621 aatttatttt caccaacact ttttttgcca caacacgaag cggcgctttt tgctataact
    10681 tagaaagtaa tataatcatc tcaggaaact attcatgcgt accacatcat ttgcgaaagt
    10741 tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc tctaaaatca cccagccaga
    10801 taaatattct ggttttttaa acaattactc tgatttaaaa gaaacaacct cagcaacagg
    10861 taaacctgtt ttacgttggg tagacccgag ttttgatcaa agcaaatatg acagcatcgt
    10921 ctggaaccca atcacttatt atccggtacc aaaaccttct acccaggtag ggcagaaagt
    10981 tctggataaa attttgaact ataccaacac cgaaatgaaa gaagcgatag cgcagcgtaa
    11041 accagtggtt accaccgctg ggccgcgtag tctgattttc cgtggggcca ttaccggtgt
    11101 agataccagc aaagaagggc tgcaattcta tgaagtggtt cctgttgcat tagtggttgc
    11161 ggggacgcaa atggctacag gccaccgtac catggatact cgcctctatt ttgaaggtga
    11221 gctgattgat gcagcgacta ataaaccggt tatcaaagtc gttcgtcagg gcgaaggtaa
    11281 agacctgaat aacgaaagta cgccaatggc tttcgaaaat attaaacaag ttattgatga
    11341 catggcgacc gatgccacca tgtttgacgt taacaaaaag tagttccaga cgcgccatcg
    11401 ttagatggcg ctttttatcc ggtgcgccgt aaaaccccat ccttcagggc ggggatataa
    11461 ggcgcggttt tccacctgac caggtgtttg ctgttgttca atgtattggc ggatgacgga
    11521 tattggcgca cctccgcagc tactggcaaa atagccagga ctccacaaaa cgcctttgta
    11581 gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt aacctacccg atacgccttt
    11641 gaggctgttt accagactgg atatcgccag tttgggaggt tagttaatta acaaatggac
    11701 gtgatctggt tcgccatcca tttcaaccag ttcagcttca aaataagcac atacatttga
    11761 aaagtaagtg cgtagttttt ctgtcgcgtc atggtcaaaa atctggcgtc ggtatctggt
    11821 gacaaagact aggtgaacat gcatcaggaa aacacaatgc cttccacgcc ggatatcggt
    11881 ttcttttttc acagaccaaa gtatcatttg acctgtgaaa cgattacagg catttaaatt
    11941 ccagttaaga ccaggtggtc aacaggagtg tgaaatgagg cgcttcgcag gcgcatgtcg
    12001 tttcgttttc aatcgtgctc tggcacgtca gaatgagaat catgaggccg gtaataaata
    12061 catcccttac gggaaaatgg cttcctggct ggttgagtgg aaaaatgcca ctgaaacgca
    12121 atggcttaaa gattctccct cacagccatt gcaacagtca ctgaaagacc ttgagcgggc
    12181 ttacaaaaac ttcttccgga agcgggctgc ttttccccga ttcaaaaagc ggggacagaa
    12241 tgctgcattc cgctacccgc aggacgttaa gctcgatcag gaaaacagcc gtatttttct
    12301 gccgaaactt ggctggatgc gctaccggaa tagccgtcag gtcacgggag ttgtgaaaaa
    12361 tgtcactgtc agccagtcct gcggtacgtg gtacatcagt attcagacag aaagtgaagt
    12421 atcaactccg gttcaccctt cagcatcaat gatcgggctg gatgctggcg tggctaaact
    12481 cgccacgctg tcagatggca cagtctttga gcctgtaaac agttttcaga aaaaccagaa
    12541 gacgctggcg agacttcagc gacagttaag ccgcaaggtc aaattcagca acaactggca
    12601 gaagcagaaa cgcaaaatac agcgactgca ttcctgtatc gcaaatatcc gcagggacta
    12661 ccttcacaaa gtcacaacgg ccgtcagcaa aaaccacgca atgatagtca ttgaggattt
    12721 gaaggtcagc aacatgtcaa agtcagcagc gggtacggtc agccagccgg ggcgcaatgt
    12781 ccgggcaaaa tcaggtttaa accgt
//
LOCUS       DAEEGA010000102        12695 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEEGA010000102 DAEEGA010000000
VERSION     DAEEGA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.102"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="KLH46_003344"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="KLH46_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBE0379918.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQT
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="KLH46_003345"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="KLH46_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HBE0379919.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="KLH46_003346"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="KLH46_003346"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBE0379920.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="KLH46_003347"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="KLH46_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBE0379921.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="KLH46_003348"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="KLH46_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBE0379922.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="KLH46_003349"
     CDS             4025..4615
                     /locus_tag="KLH46_003349"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBE0379923.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="KLH46_003350"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="KLH46_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBE0379924.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="KLH46_003351"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="KLH46_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBE0379925.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="KLH46_003352"
     CDS             complement(6551..6658)
                     /locus_tag="KLH46_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379926.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="KLH46_003353"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="KLH46_003353"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBE0379927.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="KLH46_003354"
     CDS             7648..7875
                     /locus_tag="KLH46_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBE0379928.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="KLH46_003355"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="KLH46_003355"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBE0379929.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="KLH46_003356"
     CDS             complement(9447..10703)
                     /locus_tag="KLH46_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HBE0379930.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSEPITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="KLH46_003357"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="KLH46_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBE0379931.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="KLH46_003358"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="KLH46_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBE0379932.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="KLH46_003359"
     CDS             complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="KLH46_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBE0379933.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3033 a         3244 c         3198 g         3220 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcggtc tgctccagtc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataacatt cactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct cacctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 agcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa taaccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc atcatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 ggctctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg cggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcagtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgt
//
LOCUS       DAEEGA010000103        12415 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEEGA010000103 DAEEGA010000000
VERSION     DAEEGA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12415
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.103"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..1199
                     /gene="edd"
                     /locus_tag="KLH46_003360"
     CDS             <1..1199
                     /gene="edd"
                     /locus_tag="KLH46_003360"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBE0379934.1"
                     /translation="ALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVHP
                     DSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMHL
                     VAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAGL
                     LHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKVL
                     SGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPKA
                     NGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVR
                     DGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQG
                     ATCITF"
     gene            1236..1877
                     /gene="kdgA"
                     /locus_tag="KLH46_003361"
                     /gene_synonym="eda"
     CDS             1236..1877
                     /gene="kdgA"
                     /locus_tag="KLH46_003361"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBE0379935.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(1933..3111)
                     /gene="purT"
                     /locus_tag="KLH46_003362"
     CDS             complement(1933..3111)
                     /gene="purT"
                     /locus_tag="KLH46_003362"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBE0379936.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            3245..3535
                     /gene="yebG"
                     /locus_tag="KLH46_003363"
                     /pseudo
     CDS             3245..3535
                     /gene="yebG"
                     /locus_tag="KLH46_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3602..3958
                     /locus_tag="KLH46_003364"
     CDS             3602..3958
                     /locus_tag="KLH46_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBE0379937.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            4285..4944
                     /locus_tag="KLH46_003365"
     CDS             4285..4944
                     /locus_tag="KLH46_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBE0379938.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            5154..7214
                     /gene="ptrB"
                     /locus_tag="KLH46_003366"
     CDS             5154..7214
                     /gene="ptrB"
                     /locus_tag="KLH46_003366"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBE0379939.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7211..7873)
                     /gene="exoX"
                     /locus_tag="KLH46_003367"
     CDS             complement(7211..7873)
                     /gene="exoX"
                     /locus_tag="KLH46_003367"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBE0379940.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLNSMSPELRLTLKHYLE
                     NT"
     gene            complement(7897..8553)
                     /locus_tag="KLH46_003368"
                     /pseudo
     CDS             complement(7897..8553)
                     /locus_tag="KLH46_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(8655..8885)
                     /gene="holE"
                     /locus_tag="KLH46_003369"
     CDS             complement(8655..8885)
                     /gene="holE"
                     /locus_tag="KLH46_003369"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBE0379941.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9024..9398
                     /gene="yobA"
                     /locus_tag="KLH46_003370"
     CDS             9024..9398
                     /gene="yobA"
                     /locus_tag="KLH46_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBE0379942.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            9402..10274
                     /gene="copD"
                     /locus_tag="KLH46_003371"
                     /pseudo
     CDS             9402..10274
                     /gene="copD"
                     /locus_tag="KLH46_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            10287..10628
                     /locus_tag="KLH46_003372"
     CDS             10287..10628
                     /locus_tag="KLH46_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBE0379943.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(11021..12097)
                     /locus_tag="KLH46_003373"
                     /pseudo
     CDS             complement(11021..12097)
                     /locus_tag="KLH46_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(12063..12344)
                     /locus_tag="KLH46_003374"
     CDS             complement(12063..12344)
                     /locus_tag="KLH46_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBE0379944.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         3080 a         3032 c         3137 g         3166 t
ORIGIN      
        1 tggccttact ggagtcagaa gccgcgtctt accatgcgcc gggaacatgt actttctacg
       61 gtactgccaa caccaaccag atggtggtgg aatttatggg gatgcagttg ccaggctctt
      121 cttttgttca tccggattct ccgctgcgcg atgctttgac cgctgccgct gcgcgtcagg
      181 ttacacgcat gaccggtaat ggtaatgaat ggatgccgat cggtaagatg atcgatgaga
      241 aagtggtggt gaacggtatc gttgcactgc tggcgaccgg tggttccact aaccacacca
      301 tgcacctggt ggcgatggcg cgcgcggccg gtattcagat taactgggat gacttctctg
      361 acctttctga tgttgtaccg ctgatggcac gtctctaccc gaacggtccg gccgatatta
      421 accacttcca ggcggcaggt ggcgtaccgg ttctggtgcg tgaactgctc aaagcaggcc
      481 tgctgcatga agatgtcaat acggtggcag gctttggtct gtctcgttat acccttgaac
      541 catggctgaa taatggtgaa ctggactggc gggaaggggc ggaaaaatca ctcgacagca
      601 atgtgatcgc ttccttcgaa caacctttct ctcatcatgg tgggacaaaa gtgttaagcg
      661 gtaacctggg ccgtgcggtt atgaaaacct ctgccgtgcc ggttgagaac caggtgattg
      721 aagcgccagc ggttgttttt gaaagccagc atgacgttat gccggccttt gaagcgggtt
      781 tgctggaccg cgattgtgtc gttgttgtcc gtcatcaggg gccaaaagcg aacggaatgc
      841 cagaattaca taaactcatg ccgccacttg gtgtattatt ggaccggtgt ttcaaaattg
      901 cgttagttac cgatggacga ctctccggcg cttcaggtaa agtgccgtca gctatccacg
      961 taacaccaga agcctacgat ggcgggctgc tggcaaaagt gcgcgacggg gacatcattc
     1021 gtgtgaatgg acagacaggc gaactgacac tgctggtaga cgaagcggaa ctggctgctc
     1081 gcgaaccgca cattcctgac ctgagcgcgt cacgcgtggg aacaggacgt gaattattca
     1141 gcgccttgcg tgaaaaactg tccggtgccg aacagggcgc aacctgtatc actttttaag
     1201 acgacaaatt tgtaatcagg cgagagaaaa ctctgatgaa aaactggaaa acaagtgcag
     1261 aatcaatcct gaccaccggc ccggttgtac cggttatcgt ggtaaaaaaa ctggaacacg
     1321 cggtgccgat ggcaaaagcg ttggttgctg gtggggtgcg cgttctggaa gtgactctgc
     1381 gtaccgagtg tgcagttgac gctatccgtg ctatcgccaa agaagtgcct gaagcgattg
     1441 tgggtgccgg tacggtgctg aatccacagc agctggcaga agtcactgaa gcgggtgcac
     1501 agttcgcaat tagcccgggt ctgaccgagc cgctgctgaa agctgctacc gaagggacta
     1561 ttcctctgat tccggggatc agcactgttt ccgaactgat gctgggtatg gactacggtt
     1621 tgaaagagtt caaattcttc ccggctgaag ctaacggcgg cgtgaaagcc ctgcaggcga
     1681 tcgcgggtcc gttctcccag gtccgtttct gcccgacggg tggtatttct ccggctaact
     1741 accgtgacta cctggcactg aaaagcgtgc tgtgcatcgg tggttcctgg ctggttccgg
     1801 cagatgcgct ggaagcgggc gattacgacc gcattactaa gctggcgcgt gaagctgtag
     1861 aaggcgctaa gctgtaaaag tcaaaatgcc cgatctagga tcgggcattt ttgtagcgtt
     1921 tttttgccgg gtttaaccct gtacttttac ctgtccggcg gcgtgcttcg cgcgttcaat
     1981 ggcgtcaaca acactctctg aagtagccag taccaccccc agacgacggc tgccatcaat
     2041 ttccggctta ccaaataaac gaatctgcaa atctgcgcct acggcattct gcacattatc
     2101 aaacgtgaca ttctgactgg tcagttgtgg cagaataacg gcagaagctg caggaccata
     2161 ctgacggatc ccgccaacag gaagtccgag gaaggcacgt acatgcaggg caaactctga
     2221 gagatcttga gaaattaacg tcaccatccc ggtatcatgt ggacgagggg agacctcact
     2281 gaaaatcacc tcatcaccac agacaaacag ctcgacacca aacagaccat aaccgcccag
     2341 tgccagcacc actttacggg caatctcctg cgcacgttca agggcaagcg ggctcatttg
     2401 ctgtggttgc caggattcac ggtagtcgcc atcttcctgg cgatgaccta ctggtgcaca
     2461 gaaatggacg ccatccaccg cgctgacggt tagcagggta atttcgaagt caaacttaac
     2521 gacgccttca acaattacgc ggcccgctcc ggcgcgaccg ccttgctgag cgtattccca
     2581 tgcctgagca agttgctctg cagagcgaat aaacgtctgc cccttgccgg aagagctcat
     2641 caccggtttt acaatgcagg gatagccaat ggcggcaacc gcctcgcgga aaaggctttt
     2701 gctatcggca aaacgataag tggaagtggg cagctgcagc tcttctgccg ccaggcgacg
     2761 gataccctcg cgattcatcg ttaatttcgt tgcgcgagcg caggggacaa cattcagtcc
     2821 ttcctcttca agttggatca gcatatcggt ggcaatagct tcgatctccg gcacgatata
     2881 atgtggtttt tccagttcaa ccacacggcg taatgcatca ccatcaagca tattaatgac
     2941 atgggagcga tgcgcgacat gcatggctgg tgcgtcggca tagcgatcga cggcaatcac
     3001 ctctacgccg agacgctgac actcgattgc cacttcttta cccagttcac cggagcctaa
     3061 taacatcacg cgagttgctg ccggacgcag cgcagtgcct aataacgtca taacgaatat
     3121 cccctgatta attccgctcg cagtataaac gaaaacgttt gcgtgtgtct ttattgctga
     3181 tgttgatttc tcaaccgaaa agaaatatac tgtataaaat ctcagttatt atgagaggct
     3241 gcaaatggcg gttgaagttt aatacgtagt cattcgtgag ggcgaagaga aaatgtcgtt
     3301 taccagcaaa aaggaagccg atgcttatga caaaatgctt gatacggcgg atctgcttga
     3361 cacctagctg acaaattctc cagtgcaaat ggaagacgag caacgtgaag ccctttcgct
     3421 atggctggca gaacaaaaag aggtgctgag caccattctg aaaaccggca aattaccgtc
     3481 cccacaggtc gtcggtgctg aatccgaaga ggaagatgcc tcacacgcgg cctgaagcaa
     3541 cttgcgctcc tgacaatttg taccatgctt tctcctgaat cattgtgcta tggagaaaaa
     3601 catgaaaaaa agaggggcgt ttttagggcg gttgttggtt tctgcctgcg catcagtttt
     3661 cgctgccaat aatgaaacca gcaagtcggt cactttccca aagtgtgaag gtctggatgc
     3721 tgccggaatt gccgcgagcg taaaacgtga ttatcaacaa aatcgcgtgg cgcgctgggc
     3781 agatgatcaa aaaattgtcg gtcaggccga tcccgtggcc tgggtcagtt tgcaggacat
     3841 tcagggtaaa gatgataaat ggtcagtacc gctaaccgtg cgtggtaaaa gtgccgatat
     3901 tcattaccag gtcagcgtgg actgcaaagc gggaatggcg gaatatcagc ggcgttaatg
     3961 gcttctgata attcgattga ccgttggcgg atgcggcgcg agtgccttgt ccgtcccaga
     4021 acgcaccaca atcgtaggcc tgagaagacg caacagcgtc gcatcgggca atcagcatca
     4081 aacaatgcca ctcaaaacga caaactactt tcacctaaaa tacccttatc acttgatata
     4141 cagtaaatac cttacctggc gcgaatttct ttgaataacg gcctaccgcc ccgcttattt
     4201 gcgcatcttt tgtctcgata tacattttct gacatcctca tccgctatgc tacaggcact
     4261 aaaaatggtt attgaggagc aaatatggct aactggttaa atcaactgca atccctgctt
     4321 gggcaaagca gttcttctac ctcctcgtct gcggatcagg gattgggcaa actgttagtg
     4381 tcaggcgcat taggcggact ggctgggctg ctggtcgcaa ataaatcagc acgtaaactt
     4441 cttacgaaat atggcaccaa cgcgttactg gttggcggcg gagcggtagc gggtacggtg
     4501 ctgtggaata aatacaaaga taaaattcgc gcggcgcatc aggacgaacc gcagtttggc
     4561 gcgcaaagta cgccgctgga tgagcgtacg gaacgtttga tccttgcgct ggtctttgcc
     4621 gctaaaagtg atggtcatat tgatgccaat gaacgtgcgg caatcgacca gcaattgcgt
     4681 gaagccggcg tggaagagca ggggcgtgta ctcattgagc aggcaatcga acaaccgctg
     4741 gatccacaac gcctggctac cggggtccgc aatgaagaag aggcgctgga aatctatttc
     4801 ctgagctgcg cggctattga tattgaccat tttatggaac gcagttacct gaatgcactg
     4861 ggtgacgcgc tgaaaatccc ccaggatgtt cgtgaaggca ttgaacgcga tctcgaacag
     4921 caaaaacgca ctttggcgga ataattccgc tctctggata gcctgaagtt gactatttcg
     4981 ggctatccag aaccgcacag ccatttgaac aggctgattt tctctgtctg cataacattc
     5041 gatatccgcg ccattacctg tcatcatcta agcaatgacg accctgtttc gcttgcatcc
     5101 ccggtgagtt ttgccaccct tataagatgt ttccaaccag aaagaacaat aacatgctac
     5161 caaaagccgc ccgcattccc cacgccatga cgcttcatgg cgatacgcgc atcgataatt
     5221 actactggct gcgggacgat acgcgttctc agccaaaagt cctggactac ctgcaacaag
     5281 aaaatagtta cggtcatcgg gtgatggcct cacaacaagc cttgcaggat cgcatcttaa
     5341 aggaaatcat cgaccgcatt ccgcaaagag aagtttctgc gccctacata aaaaatggct
     5401 accgctatcg gcatatttat gaaccaggct gtgaatatgc tatctaccag cgtcaatcgg
     5461 cattcagcga agagtgggat gagtgggaaa cattgctcga tgccaacaag cgcgcagctc
     5521 atagtgagtt ttattcgatg ggcggaatgg cgattacgcc cgataacacc attatggcgc
     5581 tggcagaaga ttttctttcc cgacgccagt acggcattcg ttttcgtaat ctggaaacag
     5641 gtaactggta cccggaactg ctggataacg ttgaacccag ctttgtctgg gcaaatgact
     5701 cctggacttt ctactatgtt cgcaagcatc cggtgacgct gctgccttat caggtctggc
     5761 gtcacgccat cggtacgcca gcatcgcaag atacactgat ctacgaagaa aaagacgata
     5821 cctattatgt cagcctgcat aaaacgacgt cgaagcacta tgtcgtcatt catctggcca
     5881 gcgccaccac cagtgaagtt cgcctgctgg acgcggaaat ggccgatgcc gagccgtttg
     5941 tttttctgcc gcgccgcaaa gatcacgaat acagccttga tcactaccag catcgttttt
     6001 atctgcgttc caatcgccac ggcaaaaact ttggcttata ccgtacccgt atgcgtgatg
     6061 agcaacagtg ggaagagtta attccgccac gcgaaaacat catgctggaa gggtttacgc
     6121 tgtttaccga ctggctggtg gttgaagagc gtcagcgcgg gttaaccagt ttgcgccaaa
     6181 ttaaccgcaa gacccgggaa gtcattggca ttgcctttga tgatccggcc tatgtgacct
     6241 ggattgccta caatccagaa cctgaaaccg cgcgattgcg ttatggttat tcttccatga
     6301 ccacaccaga cactttgttt gaactggata tggataccgg tgagcgtcgt gtattaaaac
     6361 aaacagaagt gcctggtttt gatgcggcga attaccgcag tgaacacctg tggatagtcg
     6421 cccgtgatgg cgtcgaagtt ccggtttcgc tggtctatca tcgcaaacat tttcgcaaag
     6481 gacacaaccc gttgctggtg tatggctatg gttcttacgg cgcaagtatt gatgccgatt
     6541 tcagttttag ccgcttgagt ttgttagatc gtggctttgt ctacgccatt gtccatgttc
     6601 gcggcggtgg tgagctgggg caacaatggt acgaagacgg taaatttctg aagaagaaaa
     6661 atacgtttaa tgattatctt gatgcctgcg atgcattgtt aaaactgggc tatggctctc
     6721 cttcgctctg ttatgcgatg ggcggaagtg cggggggcat gttgatgggc gttgcaatta
     6781 atcaacgccc ggaattattc cacggcgtta tcgcccaggt accgtttgtt gatgttgtaa
     6841 caacgatgct tgatgaatca attcctctta ccactggtga gtttgaagag tggggaaatc
     6901 cgcaggatcc gcaatattac gagtacatga aaagctacag cccgtatgac aacgtcaccg
     6961 cacaggctta tccgcattta ctggtaacga ccggtttaca cgattctcag gtgcaatatt
     7021 gggaaccggc aaaatgggtc gctaaattgc gcgagctgaa aaccgatgac catcttttat
     7081 tgctctgtac cgacatggac tcaggccatg gcggtaaatc tggccgcttt aaatcgtacg
     7141 aaggcgtagc gatggaatat gcttttctgg tcgcgctggc gcagggaaca ttacccgcta
     7201 cgcctgcgga ttaagtattt tccagataat gtttcagtgt taaacgcagc tccgggctca
     7261 tgctgttcag gttattaaat aaccagcgca gatagcccgg atagcgttcg gcaacgtcgg
     7321 aaaccgcttt gccacggtat ttgccaaagg tgaaggtcgt catcaacgac ggacgtccgg
     7381 tgatatcggc catctgttct gccgtccagc cggaggtgtt cataatatcg ataagcaacg
     7441 cggcggtgat ataacagtca tacaacgcgc ggtgatgatg caggcccggc ggtgtctgta
     7501 cattgagctt gcgtgtttta tataacgcca tattgctgta cttgagccct ggccacaaac
     7561 gacgggccag tttcatagtg caaatccact caccgggcat ctcaggcagt acgcggcggt
     7621 caaagctggc gttgtgcgcg acataccatt cactaccgta atagtgtggg atcacatctt
     7681 caatccacgg tttatcggcg accatggctt cggtgatgcg atgaatcgcc atcgcttgtg
     7741 gactaatagg acgatcgggg cgcaccagga ggctcatggg gttgacgatt tttccgtcaa
     7801 tgacatcaac agaggcaatc tcaacgatcc ctccctgcaa accgcaggtt tctgtatcga
     7861 taatgcgcaa catggcatgc tccaggccga aaaagcctag cgtaatggaa tgatatcgcc
     7921 ttgccaaccc tgtgatgaac gctgaccgac taacaataat gaaccacgat cggcgcgaac
     7981 gatgagacga ccatattcat cccatagtgc actgttacca cgggcatttg ccatcagcac
     8041 ggcaatcgag tactgatgtg aaaaaaattg taagcgacgg gccgacgcca gcaggtcggg
     8101 ttctcccaga cattgccctg tcgtaaacag tgaacaggtc gggtccatat ccataccttg
     8161 cggttgttca tcgaccaccg tgatggttct ggaacgacgg cccagacatg caccatgact
     8221 ctgatggtag ttcccaggcg tttttcgcca cggtgcgaac accgcaattc cacgaataaa
     8281 gcgatcgtta tattcaacgg gaaggccggc aatgatggtc attcgctagg tcgttgcggc
     8341 ataacaaagc gggtctaaca gtgaaagatc gggtggggca ggaagggcac gtcgcgaata
     8401 atcacacccc agtaaagaaa gcgatggaaa aactaacagc tgacattgtt gcctggcggc
     8461 ggccctcaca aactccagat gatgagctac ctgttcggtg agtgaggttt tacgaggctc
     8521 atattgcgct gctgcaactt tccagaacga cataatgact tccttttcaa aatgactgca
     8581 aattatgggg gcggtttaga aaatactcat aatttagcag gcgttatgta agaaagtgta
     8641 actctgtata agttttattt aagtttgggc tcgtaaggta aacgtgacag attgaccgaa
     8701 gccaaacggt gggcaataag ccgctcgcga aaccagctgc gcaaatgttc tggctgttca
     8761 cgttcaaccg cttcagcgat caccggcata ttgtagcgtt ctttaaaagc caccccggcc
     8821 gccgccaaat cgacattcac tttatccatt tctgtttgat ccagtttagc cagattcttc
     8881 agcattctta atctcctcat cattcgcggg caaaagttac taagagtgag cctggatggc
     8941 aagccggaat tgatgatcct cgactcagac attagacaag gttatgctct gtaaaaaagt
     9001 tattacaaaa aggaatagat aatatggctt caactgcacg ctcccttcgc tacgcgctgg
     9061 caatcctgac cacttcgttg gtgacacctt ccgtctgggc acatgcgcat ttaacgcatc
     9121 agtatcctgc ggcaaacgcg caagtgacag ctgccccgca ggcaatcact ttaaacttct
     9181 cggaaggcgt tgaaacagga ttcagtggtg caaaaatcac ggggccaaaa aacgaaaata
     9241 ttaaaacatt accagcgaag cggaatgagc aggaccaaaa gcaactgatt gttccactgg
     9301 ccgattcgct gaaacccgga acctacaccg ttgactggca tgttgtttcg gtggacgggc
     9361 ataaaacgaa aggacactac acctttagcg tgaaataaat catgctggcg tttacctgga
     9421 tcgcgctgcg atttatccat ttcacctcgt tgatgctggt ctttggcttt gcaatgtatg
     9481 gtgcctggct ggccccctta atgattcgtc gcttgctgac gaagcgttct ctgcgtttac
     9541 aacagcacgc tgccgtctgg agtttgatca gtgccactgc aatgctcgcc gttcagggcg
     9601 ggttgatggg aacgggatgg acagatgtat tttcgccaaa catttggcag gctgttttgc
     9661 aaacgcagtt tggtggcgtc tggttatggc aaattgttct cgccctcgta acgttgatcg
     9721 tcgcccttat gcaaccgcgg aatatgccac gtttgctgtt tatgctcact accgcgcaat
     9781 ttattctgct ggcgggagtg gggcatgcga cgctgaatga aggggtaaca gcgaaaatcc
     9841 atcagactaa tcacgcgatt cacctgattt gtgcggcggc gtggtttggg ggattactgc
     9901 cggtgctctg gtgtatgcaa ctcatcaaag gccgttggcg acactaggct attcaggcgc
     9961 tgatgcgttt ttcctggtgc gggcattttg cggtgatcgg cgtactggca agcggcgtgc
    10021 ttaatgcatt gttgattacc ggatttcccc ccacgcttac gacttactgg ggccaactct
    10081 tattgctcaa agccatcctg gtgatgatta tggtggtcat tgcgctggct aatcgttatg
    10141 ttcttgtacc gcgcatgagg caggatgaag atcgtgcggc accctggttt gtgtggatga
    10201 ctaaactcga atgggcgata ggtgcggtag tgctggtgat catcagcctg cttgcgactc
    10261 tcgaaccttt ttgatggatt acgaaaatga tgaaaaaaag tattctggcg tttctgttac
    10321 tcaccagttc tgcagcggcg ctggcggctc cacaggtgat aaccgtgagt cgttttgaag
    10381 tgggtaaaga caaatgggcg tttaatcgcg aagaggtaat gctgacttgc cgaccgggta
    10441 atgctttgta tgtcatcaac ccaagtaccc tcgtgcagta tcctttaaac gatatcgcac
    10501 aaaaggaagt tgccagtggg aagaccaatg cccaacccat ttcggtgatt cagattgatg
    10561 atcctaacaa tcccggcgaa aaaatgagtc tggcaccgtt tatagaacga gctgaaaaac
    10621 tctgttaatt acctaaaata gccttttgat ttccaataaa aaaaccgcct cagttctttc
    10681 accagaacgg gcggttttta acatttaagc tgatgagcac cgcgcttttt attgaccatt
    10741 ttgcacgcaa actggaaaac ctggcgtcgt catctattct taaagggcaa ggcaactaag
    10801 cctgcattaa tgccaacttt tagcgcacgg ctctctccca agagccattt ccctggaccg
    10861 aatacaggaa tcgtattcgg tcttttttta attgtattta aaatcaatta gttgcaaacg
    10921 tctccccgaa attccccgaa atttactcga atttctgtat tccggtcttt tttggttata
    10981 tcacaaccaa aatacattta acaatccatt tacgttaaaa tcagagcagt aagtacgttt
    11041 tttctctctc atcaagatac atttttgttg tcttctccga tgtgtggcca agtagacgct
    11101 gagcaaattc ttctccacat atttctttgt acaatcgtcc agccaggctt ctgatttcgt
    11161 gaaaagttgg tgggttttca ctgaacagga tacctgttaa ttttctggct gcgacaaatt
    11221 tctttgtcag gccgtccggg tgaatgctgc cgtcagggct gttttttcta atcccggcac
    11281 tgattagata atccccccta cttaccatgc ggcactgttc aactactgca ccaagtcgta
    11341 aaccagcgac aggaaggctg agtgacaggg ggatagcaat catcattcct gtcttaattt
    11401 gcctgatgtg gagacgatca tcataaatat cactgaaccg catattcgtt atgtcttcgc
    11461 gacgttgtcc tgttacaagg gctaagtcca tagctaatgg gaaccatgcc ggaagttgat
    11521 ctgctgcctc cctgatgcag ttgtatgtct ttagtttcag ccgttctctt gtaactacta
    11581 ttttcggtgc tcttgttggc gttactggat tttgagatat acgtccttca acaatggctt
    11641 cgcggaacat atcagataac acagaacgca ttgatcctgc catcgtgttt ttccctcctt
    11701 caatctacaa atcaagaaac tcggcgatat ggcgagtggt tatttctgtc agtaaaaccc
    11761 ctcctaattt ttcttttatt gtctccagtt gatttacccg agttttataa gtatttctgg
    11821 acgcttttct ccttataaga atcgttttgt aacgttcaat ccagcctgcc atagtaaatg
    11881 agtcgacccc tttaagcttt tcaattaggg cagcaggaga gtagtttttg tatatataat
    11941 gatttgcttc aattgcctgc gctactgcat ctcttcttga aattttacct agtgtaaatt
    12001 cttctttcgt cagagggttg cgccagtaat atgctttgtc tctccttcga tatgttaagt
    12061 ttctaggcaa attgggatcg tattttttcc gctgcatgtt ttaacttctc cattaacgga
    12121 ctgtctctcc cttgtcgccc attaggctga tggtgtgtta tatcggtatc aaccttattt
    12181 gggttgatat agaaagcctc cggaaccacc ctgtaactcc tcccgtgtag ttcaggtgca
    12241 ggataaatgt ttccattcct tgcccatcgt ctcagcgttg atattgatgg tgggttatcc
    12301 ggatatctga gttttcccca cgttttgagt gtcacaagat tcattgccat acctctcacg
    12361 atatgaccgc cagtaaatat acagaatact ggcgggtgtg gttgattttt aataa
//
LOCUS       DAEEGA010000104        12222 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEEGA010000104 DAEEGA010000000
VERSION     DAEEGA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12222
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.104"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..495)
                     /locus_tag="KLH46_003375"
     CDS             complement(<1..495)
                     /locus_tag="KLH46_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0379945.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAH"
     gene            complement(526..1218)
                     /gene="fimC"
                     /locus_tag="KLH46_003376"
     CDS             complement(526..1218)
                     /gene="fimC"
                     /locus_tag="KLH46_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBE0379946.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1438..1980)
                     /gene="fimA"
                     /locus_tag="KLH46_003377"
     CDS             complement(1438..1980)
                     /gene="fimA"
                     /locus_tag="KLH46_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBE0379947.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2461..3327
                     /gene="folD"
                     /locus_tag="KLH46_003378"
     CDS             2461..3327
                     /gene="folD"
                     /locus_tag="KLH46_003378"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBE0379948.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKLGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3329..3541
                     /gene="ybcJ"
                     /locus_tag="KLH46_003379"
     CDS             3329..3541
                     /gene="ybcJ"
                     /locus_tag="KLH46_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBE0379949.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3649..4158
                     /locus_tag="KLH46_003380"
     CDS             3649..4158
                     /locus_tag="KLH46_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBE0379950.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4194..5579)
                     /gene="cysS"
                     /locus_tag="KLH46_003381"
     CDS             complement(4194..5579)
                     /gene="cysS"
                     /locus_tag="KLH46_003381"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBE0379951.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5753..6247
                     /gene="ppiB"
                     /locus_tag="KLH46_003382"
     CDS             5753..6247
                     /gene="ppiB"
                     /locus_tag="KLH46_003382"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBE0379952.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6250..6972
                     /gene="lpxH"
                     /locus_tag="KLH46_003383"
     CDS             6250..6972
                     /gene="lpxH"
                     /locus_tag="KLH46_003383"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBE0379953.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7090..7599
                     /gene="purE"
                     /locus_tag="KLH46_003384"
     CDS             7090..7599
                     /gene="purE"
                     /locus_tag="KLH46_003384"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBE0379954.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7596..8663
                     /gene="purK"
                     /locus_tag="KLH46_003385"
     CDS             7596..8663
                     /gene="purK"
                     /locus_tag="KLH46_003385"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBE0379955.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYACGVIWAQSKFS"
     gene            complement(8800..9693)
                     /locus_tag="KLH46_003386"
     CDS             complement(8800..9693)
                     /locus_tag="KLH46_003386"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBE0379956.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9690..10337)
                     /locus_tag="KLH46_003387"
                     /pseudo
     CDS             complement(9690..10337)
                     /locus_tag="KLH46_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10340..11572
                     /gene="allC"
                     /locus_tag="KLH46_003388"
     CDS             10340..11572
                     /gene="allC"
                     /locus_tag="KLH46_003388"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBE0379957.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11583..>12222
                     /gene="allE"
                     /locus_tag="KLH46_003389"
     CDS             11583..>12222
                     /gene="allE"
                     /locus_tag="KLH46_003389"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBE0379958.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2969 a         3155 c         3118 g         2980 t
ORIGIN      
        1 gtgagcgcta ctcaacaacg ctatctgagg aatagtgatg ttcaggcgca tcgctgcaaa
       61 atcaaagtta attgtggcat caggtacagc tttcagtaaa ttgatgcatt tattgttttg
      121 ctgctgggct aactcgggaa atgcactgct attaaggcca attcgttcaa ggagtacctg
      181 gttaaaacag ggcattaacc cacctgattt atcacctgta ttttctgttg tcgattcaaa
      241 tacgatatcc tgtgaaccaa tgaactcatc attacgccag agatccaccc gatacacgcc
      301 cgcaggttga tgattacctc tctcaaagcg cgataaatca gcaacggatg tgccattctc
      361 taataaaaat gccgggttga aataactttc cgcatagctt aaaaaggagg gtaaaatggc
      421 aagagatgaa taacaaattc cggccagaca ccaggtatac ctctgcggaa tatccgtagt
      481 agtgggtatt ttcataatta gctctgatat tgtcagatga ttcggttagt ttaagttcac
      541 ttctctgacc ggggttactg aaccataatc attaacggtc tgaaatttca gctttccctg
      601 cactcctgag ggtaagggaa tttgtgaact aactctgggt gccgccatag cattccccaa
      661 cttttggctg cccacttgca ggttaacgag ggtgacataa aaaggtgatg gattatgaac
      721 attgatatag ttaccggatc gcgaaaactt gagtgtgtcc ggcgcttctg cgggtaattc
      781 ttgtaattga attggacgga gaaataattt catgcgcgat aaaatcgcca gttgcaaaac
      841 attcctgccg ttcaatgcat ttttatctac cgaagggatc gtcttaacat tcatccagaa
      901 cagagactca cgatctgccg ccagcggtgg accggtgtaa ataatacgca aagtattttc
      961 gcttttgggt tcactaacaa atagtggcgg tgtaatgatg aatgactttt cttttacacc
     1021 gctgctgttt tcaatccacg aattgaccag aaagagctca ttggtatggc tatttttaat
     1081 ccataccgca gtctgtttag catcagcggg ataaataata cgcgtggcac ctaatgcgat
     1141 ccctccggca gcatgggcgc tggccgaaat gattaaataa aatatcatga gcatcaataa
     1201 ctttatttta gtcatcatag ttaatacgcc ttgtgtattc ctgacggcaa aaataaggag
     1261 tataaagaaa tgattgcttt ggcttcttac aacacatcat tcccttttca tttaataaag
     1321 cggcatgata caaagtgaac gtggtttcga tttacattgc caccataccg cttcagacaa
     1381 ccggtacatt actttgttat aagacgtgat atataattca aaacaacgtg gttttgatta
     1441 ttcgtatctc ataataaaag tcgcgtcagc atttgcttgc cctgctgatg cacttgtacc
     1501 ggtgccttta taacgtgcag aaaaatgaag aacgttggtc ccggggatca agttctggtt
     1561 ggttgagaag ctattcccat caggtttgag aattgcggat gtattatcaa gtatttcaat
     1621 accgacaccc gttgctgttg ttgtatttgt actggatgca atggccaata aattatcatt
     1681 gattgcatca gcctgaccag aaaatgcaac ggcagcatta gcggcaacaa caggatcgca
     1741 gtcgttcaac tgaatggtga atggaattaa tgcagaggta ttaccaacag cattgaaaat
     1801 atcggtacga tattgaccga gtgtgacaac ctggtctgcc gagtcagtat tcactgaaca
     1861 ggcagcattc accagttcgc cttcaaagtg aattgtaccg ccatctacaa cggcagcata
     1921 actacccgta gcggcgaata gtgcggcagc cagcgcagac gaaataaatc ttaatttcat
     1981 atatattcct tcaatctcat ttatcgactc cacatccgta tataaccgat tactttattt
     2041 aagacactga tagtagtaaa attccttttt atcctctaag aatgtcttaa ttgaaaatat
     2101 gcactctatt ctaaaaaata gagagccccg ttagatgaat acttccgcgc aaaatatatt
     2161 caacacaaat atagacctga agcggtaaat taccaggctg aaaattcttt ttatattgtc
     2221 aggtatttct taaattatct taatccttaa acaaggaaat aaatcagttc cagatttaca
     2281 acgccatcat gtacgaaaaa tgaagctttc aatctcagcg acctcagcga cggtgcgcct
     2341 aaccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc
     2401 gcttctgaca aaatagacgc atccccttcg acctacgtaa cagatggaat cctctctctg
     2461 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
     2521 caaaaagttc aggctcgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
     2581 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
     2641 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgctg
     2701 gagcttatcg atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
     2761 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg
     2821 gacggtttcc atccttacaa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc
     2881 tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctc
     2941 aacgccgtgg tgattggcgc atcgaatatc gtcggtcgcc caatgagcat ggaactgttg
     3001 ctcgcaggtt gcaccaccac cgtgactcac cgcttcacta aaaatctgcg tcatcacgta
     3061 gaaaatgccg atctgttgat cgttgccgtt ggcaagctag gctttattcc cggtgactgg
     3121 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
     3181 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccc
     3241 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgctaca ggcgtgcgtt
     3301 gaatatcatg atccacaggg tgagtaacat ggcgacattt tctttaggta aacatccgca
     3361 cgttgagctg tgcgacttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     3421 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     3481 caaaatcgtc gccggtcaga cagtgagttt tgcaggacac agcgtacagg ttgttgcctg
     3541 atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc
     3601 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     3661 attacgcacg ccgccgttcc cctttgcatt ggtttagggt tgggttcgaa aggtctgtta
     3721 tttgccggaa tcatcctggc gatgctgccc gatgccgacg tattgtcgtt taaatttggc
     3781 gttgcttacg gcaatgtttt tggtcatcgc ggttttaccc attcgctggt gtttgcgttt
     3841 gttgtcccgc tcttatgtgt gctgattgga cgtcgatggt tcagggctgg gctgattcgc
     3901 tgctggctgt ttttaaccgt ctcgttgcta tcgcacagct tactggattc ggtaaccact
     3961 ggcggtaagg gcgttggctg gctgtggccg tggtcagatg aacgcttttt cgctccctgg
     4021 caggtgatta aagtcgcgcc gtttgcattg tctcgttaca ccacagcgta cgggcatcag
     4081 gtgattattt ccgaattgat gtgggtgtgg ttgccgggga tgctactgat gggaatgttg
     4141 tggtggcgca gacgataacc ggatgcgaaa actcgcatcc ggcaattgcg caattactta
     4201 cgacgccagg tggtcccttg cgggccatct tccagcacga tccccatctc gttaagacgg
     4261 tcacgtgccg catctgccgc cgcccagtct ttcgctttac gggcatccag acgctgttga
     4321 attaacgctt caatctcagc cacttcgctg tcgtctgcct gcgcgccgct ttgcaggaac
     4381 gcttccggtt cttgctccag caggcccaat acagcggaaa gtttacgcag gtgagatgcc
     4441 attgcattcg ccgctgccat atcttctgct ttcagacggt tcacttcacg cgccatatca
     4501 aacagcacgg aataggcttc cggggtgttg aaatcgtcgt ccatcgcttc aataaagcgc
     4561 gcttcaaacg cttcgccacc ggcaggcgca acggttttat ctgtgccgcg cagcgcagtg
     4621 tagaggcgct ccagcgccgc acgcgcctgc ttcaggttct cttcgctgta gttcagctgg
     4681 ctgcgatagt ggcccgacat caggaagtaa cgcacggttt ccgcgtcgta gtatttcagc
     4741 acatcgcgca cggtaaagaa gttacccagc gatttggaca tcttctcgcg gtcaaccatc
     4801 accattccgg agtgcatcca gtagttcaca tactgaccat catgggcaca ggtggactgc
     4861 gcgatttcgt tctcgtggtg cgggaacatc aggtctgaac cgccgccgtg gatatcaaag
     4921 tggttaccca gctgcttgca gttcatggcc gaacattcaa tgtgccagcc aggacgaccc
     4981 gcgccccacg gagacggcca gctcggttcg ccctctttcg acatcttcca cagaacgaag
     5041 tccatcgggt tgcgtttgtc atcaaccacg tcaacgcgcg cgccagcctg cagctggtcg
     5101 agatcctgac gcgacagcac gccataagtt ggatcggtcg ggacgtcgaa catcacgtcg
     5161 ccgttgtccg ccacataagc gtgacctttg gcgatcagtt gttcagtgag ttcaataatt
     5221 tctgcgatat ggtgcgtcgc gcgcggctcc atatccgggc gcagaatgtt caaagcatca
     5281 aaatctttgt gcatttcggc gatcatgcga tccaccagcg ccacaaagct ttcgccattt
     5341 tcattggcgc gtttgatgat tttgtcgtcg atatcggtaa tgttgcgcac atacttcagt
     5401 ttatagccga ggaaacgcag atagcgcgca accacgtcaa aagcaacaaa ggtacgcccg
     5461 tgaccgatat gacagagatc gtaaacggtg attccacaca cgtacatgcc gacttccccg
     5521 gcgtgaatag gcttaaattc ctctttttgg cgtgtcagag tattgaagat ttttagcatc
     5581 gaagattccg tttagacatg tgtgggtaat tgagttgcgt ataataccca tatttcccgc
     5641 gcgaatcagc atacattgcg tgatgatccg atcgtgcggt tatgctataa caccacccta
     5701 tatatgaccc gaactgggtt gaagcaccaa tcaaacggaa caggatgcaa aaatggttac
     5761 tttccacacc aatcacggcg atattgtcat caaaactttt gacgataaag cacctgaaac
     5821 agttaaaaac ttcctggact actgccgcga aggtttttac aacaacacca ttttccaccg
     5881 tgttatcaac ggctttatga ttcagggcgg cggttttgaa ccgggcatga aacaaaaagc
     5941 caccaaagaa ccgatcaaaa acgaagccaa caacggcctg aaaaataccc gtggtacgct
     6001 ggcaatggca cgtactcagg ctccgcactc tgcaactgca cagttcttca tcaacgtggt
     6061 tgataacgac ttcctgaact tctctggcga aagcctgcaa ggttggggct actgcgtgtt
     6121 tgctgaagtg gttgacggca tggacgtggt agacaaaatc aaaggtgttg caaccggtcg
     6181 taacggtatg caccaggacg tgccaaaaga agacgttatc attgaaagcg tgaccgttag
     6241 cgagtaatcg tggcgacact ctttattgca gatcttcatc tctgcgtgga agaaccggcg
     6301 atcaccgccg gttttctgcg ttttttagcg ggggaagccc gcaaggccga cgcgctgtat
     6361 attcttggcg atctgtttga agcatggatt ggcgacgacg atcccaaccc actccatcgc
     6421 cagatggcgg cagcgatcaa agcggtgtcc gattccggcg ttccctgtta tttcattcat
     6481 ggcaaccgtg attttctgct cggcaaacgc tttgcccgtg aaagcggcat gacattattg
     6541 ccggaagaaa aggtgctcga actttatggt cgccgggtgt taattatgca tggcgacacg
     6601 ctgtgcaccg atgacgcggg ttatcaggct tttcgcgcca aagtccacaa accctggctg
     6661 caaatgctat tcctcgccct gccgttgttt gtgcgcaaac gcattgccgc gcgaatgcgc
     6721 gcgaacagca aagaagccaa cagcagtaaa tcgctggcga tcatggacgt taaccaaaac
     6781 gctgtggtca gtgcgatgga aaaacatcag gtgcaatggc taatccacgg gcatacccat
     6841 cgcccggcgg tgcatgaact tatcgccaac cagcaacctg cttttcgcgt ggtactgggt
     6901 gcctggcata cggaaggttc gatggtgaaa gtcacggctg atgacgttga gctgattcat
     6961 tttccgtttt aaaaaaccca caactttgct gatttcacag ccacgcaacc gttttccttg
     7021 ctctctttcc gtgctattct ctgtgccctc taaagccgag agttgtgcac cacaggagtt
     7081 ttaagacgca tgtcttcccg caataatccg gcgcgtgtcg ccatcgtgat ggggtccaaa
     7141 agcgactggg ctaccatgca gttcgccgtc gaaatcttcg aaatcctgaa tgtcccgcac
     7201 cacgttgaag tggtttctgc tcaccgcacc cccgataaac tgttcagctt cgccgaaagc
     7261 gccgaagaga acggttatca ggtgattatt gcgggcgcag gcggcgcagc gcacctgcca
     7321 ggcatgattg ccgccaaaac gctggtgccg gtgctgggcg tgccagtaca gagcgccgca
     7381 ctgagcggtg tcgatagcct ctactccatc gtacaaatgc cgcgcggcat tccggtgggt
     7441 acgctggcga ttggtaaagc tggcgcggca aacgcggcat tactggcagc acaaattctc
     7501 gcgactcacg ataaagagct acaccagcgt ctgaatggct ggcgcaaagc ccagaccgac
     7561 gaagtgctgg aaaacccgga cccgcgaggt gcggcatgaa acaggtttgc gtcctcggta
     7621 acgggcagtt aggccgtatg ctgcgtcagg caggcgaacc gttaggcatt gctgtctggc
     7681 cggtcgggct ggacgctgaa ccggcggcgg tgccttttca acaaagcgtg attaccgctg
     7741 agatcgaacg ctggccggaa accgcattaa cccgcgagct ggcgcgtcat ccggcctttg
     7801 tgaaccgcga tgtgttcccg attattgctg accgtctgac tcagaaacag cttttcgata
     7861 agctccacct gccgaccgcg ccgtggcagt tacttgccga tcgcagcgag tggcctgcgg
     7921 tgtttgatcg tttaggtgaa ctggcgattg ttaagcgtcg cactggtggc tatgacggtc
     7981 gcggtcaatg gagtttacgc gccaatgaaa ccgaacagtt accggcagag tgttacggtg
     8041 aatgtattgt cgagcagggc attaacttct ctggtgaagt atcgctggtt ggcgcgcgcg
     8101 gctttgatgg cagcaccgtg ttttatccgc tgacgcataa cctgcatcag gacggtattt
     8161 tgcgcaccag cgtcgctttt ccgcaggcca acgcacagca gcaggcgcaa gccgaagaga
     8221 tgctgtcggc gattatgcag gagctgggct atgtgggcgt gatggcgatg gagtgttttg
     8281 tcaccccgca aggtctgctg atcaacgaac tggctccgcg tgtgcataac agcggtcact
     8341 ggacacaaaa cggtgccagc atcagccagt ttgagctgca tctgcgggcg attaccgatc
     8401 tgccgttacc gcaaccagta gtgaataatc cgtcggtgat gatcaatctg attggtagcg
     8461 atgtgaatta tgactggctg aaattgccgc tggtgcatct gcactggtac gacaaagaag
     8521 tccgtccggg gcgtaaagtg gggcatctga atttgaccga cagcgacaca tcgcgtctga
     8581 ctgcgacgct ggaagcctta atcccgctgc tgccgccaga gtatgcctgc ggcgtgattt
     8641 gggcgcagag taagttcagt taagcaggtg ctcatttgat tgccggatgc ggcgtaaacg
     8701 ccttatccgg cttaccgctc cggtacccat tgtaggcctg ataagacgcg tcaagcgtcg
     8761 catcaggcat ggtgcaccga ttgccggatg ctctcatcac tacagcgaaa tacaggtccc
     8821 cgcttcgccc gccagcgtct cttcaattcg cgataacgcc ccaatccacg cgggtttact
     8881 acggcttctg acataaccac tcaccgccgt taccttcggc cccatcgaac catcggcttt
     8941 ggcaaatgga gctaactcat ccggtgtggc atggcgaatg gcacgttgct gcggcgttcc
     9001 ccagttttca tataccgcat cagcatcggt gaggatcacc agtccatcgg cattaatctg
     9061 ctcggcgagc aacgcagcgg cgagatcttt atcaatcacc gcttcactcc ctgccccatc
     9121 ttccgtcaca ggcacaccgc cgccgccact gcaaatcacc acatgcccct ctttgagcaa
     9181 caactcgatg gcttcgctgt cgagaatttt acgcggttgc ggagacgcca ccacccggcg
     9241 caaatattta ccatcacgtt tcatctgcca gccgtaagcc gcttccagtg cctcttgttc
     9301 ttctggctga taaaccggac caataaattt ctctggctgc aaaaacgccg gatcatcagg
     9361 cgaaacctca atgcgcgtca gcaccgtcgt aacaggcggc atctgcggct gtgcgctcaa
     9421 gctctgcgcc agcatatagc caatcatccc ctggctttcc gccaccagta catccagcgg
     9481 atacggttct acctctttcc acgccagatt ctgtaacgcc agcagcccca cctgcggccc
     9541 gttgccgtga acaatcgcca gccgataaga acgggccagg cgtgccagcg cgggtacagc
     9601 actggcgata ttgcgatatt gattttccgc cgtcagcgcc tcaccgcgct ggagtaaggc
     9661 gttgccccca agagctacaa ccagtgtttt catggttttc cttgtaataa ttgttggcca
     9721 agccagaacc ccagcagcgt atctgcccct gacgtatgcc ccagcgccag cagcgaatca
     9781 atcgcaacag cggtacgttt cgggcaactc agagcatgga caaagtgcag gagtggcgag
     9841 gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc tcaccgctgt ggtaacgagt
     9901 tgaagattgt cggaacaggc aaaaaacggg cgaccggcgc gcgcatctaa agcgccataa
     9961 taccaggccg ccagcagcat tccgctcaac gtgtcatcat gacttggcgt taatccgggg
    10021 cctttaccca gccagtaccg ccagtcggtc ttaacgccat tgagcgcggc ctgaaaacag
    10081 tgacgaaact ggcgtaattc agcgggcagc ggatcgcttg ccgccatcgc cagtggcccg
    10141 aaaagtccgg tttcctccgc gcgttgcatc catgcagctg caagcggttg aggatgcgaa
    10201 ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt taaccgtgaa acgcccgagg
    10261 cgaatccctt gagccacaac ctgtggtcgt tcattgccgc ataatccacc gcataacgca
    10321 tcgaattgcg tgcggcgaaa ttacacattc ccgtcaagct atagaagaaa cgctgccctg
    10381 gctttcctct tttggcgctg acccaacggg tgggatgacc cgtttacttt attcgccgga
    10441 atggctggaa acccagcagc aatttaaaaa aagaatggca gcaagtgggc tggaaacacg
    10501 tttcgatgaa gtggggaatt tatacggtcg cctgagtggc accgaatatc cacaggaagt
    10561 ggttctgagc ggttcgcata tcgataccgt ggttaacggc ggtaacctcg acgggcaatt
    10621 cggcgcgctg gcggcgtggc tggcaattga ctggttgaaa acgcaatacg gcgcaccgtt
    10681 acgtacggtc gaagtggtgg cgatggcaga agaagaaggc agccgcttcc cgtatgtctt
    10741 ctggggcagt aaaaatatct ttgggctggc gaatcctgac gacgtgcgga atatctgtga
    10801 tgccaaagga aatagttttg ccgatgcgat gaaggcttgc ggatttactc tgccgaacgc
    10861 cccactaact ccgcgtcagg ctataaaagc ctttgttgaa ctgcatattg aacagggctg
    10921 tgtgctggaa agtaatgggc aatcaattgg cgtggtgaat gcaattgtcg ggcagcgtcg
    10981 ctatacggtg acgctgaacg gcgaatcaaa ccatgcaggc accacgccga tgggttatcg
    11041 tcgtgatacg gtttacgctt tcagtcgcat ttgccatcag tcgatcgaaa aagcgaaaaa
    11101 gatgggcgat ccgctggttc tgacctttgg caaagtagag ccgcgcccga atacggtaaa
    11161 tgtggtgccg ggtaaaacca cgttcaccat tgattgtcgt cataccgacg ctgccgtgct
    11221 gcgcgatttc acccaacagt tagaaaacga catgcgggcg atttgcgatg aaatggacat
    11281 tggtattgat atcgatttat ggatggacga agaacccgtg ccgatgaata aggagctggt
    11341 cgccaccctg acagaattgt gtgaaagaga aaaactgaat taccgggtga tgcacagtgg
    11401 tgccgggcac gacgcgcaaa ttttcgcgcc tcgcgtacca acctgcatga tttttatccc
    11461 cagcatcaac gggatcagcc ataacccggc ggaacgcacc aatattactg accttgccga
    11521 aggggtcaaa acgttggcac tcatgcttta tcaacttgcc tggcagaaat aaggagtcat
    11581 aaatgggata tttaaataac gtcaccggtt accgcgaaga tttactggct aaccgtgcga
    11641 ttgttaaaca cggtaatttc gcactgttaa ccccggatgg tctgataaaa aatattattc
    11701 cggactttga aaattgtgac gcgacaatcc tctccacgcc aaagctgggt gcctcttttg
    11761 ttgattatct ggtcacactg catcaaaacg gtggcaacca acagggcttc ggtggcgaag
    11821 gcattgaaac gttcctgtat gtgatctctg gaaatatcac tgccaaagcc gaaggcaaaa
    11881 catttgcctt aagcgaaggt ggctatcttt attgcccacc aggctcctta atgacgtttg
    11941 ttaacgccca ggccgaagac agccaaatct ttttatataa gcgccgctat gttccggtag
    12001 aaggccatgc accgtggctg ttttctggca atgccagcga actggaacgc attcactatg
    12061 aaggtatgga tgatgtgatc ctgctggatt ttctgcccaa agagttaggt tttgatatga
    12121 acatgcatat cctctctttt gcaccaggtg ccagccacgg ttatatcgaa acacacgttc
    12181 aggaacatgg tgcctatatt ctttccggtc agggggttta ta
//
LOCUS       DAEEGA010000105        12153 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEEGA010000105 DAEEGA010000000
VERSION     DAEEGA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.105"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            126..995
                     /locus_tag="KLH46_003390"
     CDS             126..995
                     /locus_tag="KLH46_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HBE0379959.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="KLH46_003391"
     CDS             complement(1198..1551)
                     /locus_tag="KLH46_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBE0379960.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="KLH46_003392"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="KLH46_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBE0379961.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="KLH46_003393"
     CDS             complement(3379..3672)
                     /locus_tag="KLH46_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBE0379962.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="KLH46_003394"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="KLH46_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBE0379963.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="KLH46_003395"
     CDS             complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="KLH46_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBE0379964.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="KLH46_003396"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="KLH46_003396"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBE0379965.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="KLH46_003397"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="KLH46_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBE0379966.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="KLH46_003398"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="KLH46_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBE0379967.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="KLH46_003399"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="KLH46_003399"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBE0379968.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGSKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="KLH46_003400"
     CDS             11052..11627
                     /locus_tag="KLH46_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE0379969.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="KLH46_003401"
     tRNA            11734..11809
                     /locus_tag="KLH46_003401"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2932 a         3140 c         2980 g         3101 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcca tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc gcgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtagtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctgg cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggctgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 aaatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa caa
//
LOCUS       DAEEGA010000106        11976 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEEGA010000106 DAEEGA010000000
VERSION     DAEEGA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11976
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.106"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..114
                     /locus_tag="KLH46_003402"
     CDS             <1..114
                     /locus_tag="KLH46_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0379970.1"
                     /translation="GPLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            111..512
                     /locus_tag="KLH46_003403"
     CDS             111..512
                     /locus_tag="KLH46_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0379971.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            523..1263
                     /locus_tag="KLH46_003404"
     CDS             523..1263
                     /locus_tag="KLH46_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HBE0379972.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1322..1708
                     /locus_tag="KLH46_003405"
     CDS             1322..1708
                     /locus_tag="KLH46_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBE0379973.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1717..2046
                     /locus_tag="KLH46_003406"
     CDS             1717..2046
                     /locus_tag="KLH46_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBE0379974.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2018..4756
                     /locus_tag="KLH46_003407"
     CDS             2018..4756
                     /locus_tag="KLH46_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBE0379975.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARAAQLGVSSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGN
                     FGALRGSGITLANRAGWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFN
                     RQLALTGHYAGVTVGQLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKA
                     AAQMERSVGQSVSDTINQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSS
                     DAARIAMSALAEETGKRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQ
                     IDALQEKIQRAKKYPWTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQK
                     RRNAENAALNRMNETEAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTR
                     ATRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDG
                     KKLTADEKSVLARKNELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERAL
                     REQHNLDIATAGMGDKQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEE
                     VLKGSLKRQLNENKRYWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFS
                     SAGNALATFCTTGKLNFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGV
                     YQSADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSG
                     MVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4756..5085
                     /locus_tag="KLH46_003408"
     CDS             4756..5085
                     /locus_tag="KLH46_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0379976.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5085..5783
                     /locus_tag="KLH46_003409"
     CDS             5085..5783
                     /locus_tag="KLH46_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBE0379977.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5788..6531
                     /locus_tag="KLH46_003410"
     CDS             5788..6531
                     /locus_tag="KLH46_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0379978.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6468..7070
                     /locus_tag="KLH46_003411"
     CDS             6468..7070
                     /locus_tag="KLH46_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBE0379979.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7131..10611
                     /locus_tag="KLH46_003412"
                     /pseudo
     CDS             7131..10611
                     /locus_tag="KLH46_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            10679..11278
                     /locus_tag="KLH46_003413"
     CDS             10679..11278
                     /locus_tag="KLH46_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBE0379980.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11342..>11976
                     /locus_tag="KLH46_003414"
     CDS             11342..>11976
                     /locus_tag="KLH46_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBE0379981.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT         2820 a         2839 c         3831 g         2486 t
ORIGIN      
        1 ggaccgctga cacaggcatt tgaagatgcc cgcgaccgca tcattgctgc ggaaatgccg
       61 aaacagctgg ggtatgccct gaaacaacaa ctgaggttac atctgagtaa atgaaccgac
      121 acacacaaat ccgtcagagc gtgctggcac gccttcggga acagtgtgga gacagcgcca
      181 cgttttttga cgggcttccg gcatttattg atgcgcagga actgcctgcc gtggcggtgt
      241 ggctgagtga tgctcagtac accggaaaaa tgacggatga agatgactgg caggctgttc
      301 tgcatattgc cgtcttcatc cgggcacagg caccggattc agagctggat atgtggatgg
      361 agagcaccat tttcccggcc ctgaatgatg taccggcact ttccggactc atcgacaccc
      421 tgaacccact cggttttaac tatcaacgtg ataatgagat ggctacctgg gcgatggcgg
      481 aaatcacgta ccagatcacg tacacgaatt aaggaggtgg taatgaccac accaaatcca
      541 ctggcaaaga cgaaaggtgc ggggacgacg ttctggatgt ataccggcaa cggcgatgcg
      601 tttgcgaacc ctttgtcgga cactgactgg ctgcgtcttg cgatggtgaa ggatctgcaa
      661 cctggcgaaa tgaccgctga tgcagaagat gacacttatc tcgatgatga agatgcagac
      721 tggaaaacga caacccaggg gcagaaatcc gttggtgata cttcggcgac gctggcctgg
      781 cgtccgggtg acagcgggca gaaaaaactg gttcagttgt tcgactccgg tgaagtctgc
      841 gcgtttcgta tcaaatatcc caacggcact gttgatgttt tccgcggctg gctgagctca
      901 ctgggtaaaa ccattgcctc aaaagacgtg atgacccgca ctgtgaaaat cagcggtgtg
      961 gggcgtccgt atctggcaga ggaaggcact gaaaccgtgg gtgttaccgg actgacggtg
     1021 gcaccggcat ctgtcagtgt caaagcggga gcaaccacca cactgacctt tacagtaaaa
     1081 cctgacgggg ccagtgacaa agcgatcagt gtgcattcgt cagatcctca gactgcttcg
     1141 gtgaccctga gcgggcttgt ggccacggtg aaaggcgtga agcagggcag tgtcagcatc
     1201 gtgggcatga cctctgacgg agagtttgtg gcagtggctg cggtgaccgt cagcgcacca
     1261 taacaggacg atactcatca ttgccccggt tatccggggc tttttgcatc cggagaacat
     1321 gatgtttctg aaacaggata cgtttaatta tgaaaaacag tccgtggtgc tcagtgagct
     1381 gtccgggctg cagagaattg aatatctgac gtttgttcag cagcgaacgg caaagtttga
     1441 tgcacaggag ggagaactgc cggaggctga acgacagatt gcttttctgc gtatgggaat
     1501 ggatatcaat gcctggctgg tttcccgctc actgtggaat gctgagcagt ctcaggatgt
     1561 tgagacgctt tgcgcatcca ttatgacaac atggtcgtat gatgcgctgg gcgcgggggc
     1621 ggagagggtt ctgtcgctga gcggtatggg gaccattgag aatgccgggg atgatgatca
     1681 tgaggcgctg acgccggaaa agtcctgacg cgggaaatgc agtttgtcat gcggcttgcc
     1741 cgggagttcc ggcgggcaga ctggcggcgg atgctgtcgg atatgtcggc cactgagctt
     1801 ggtgagtggg gcgattattt ccggatgcag agcttcagtg atgtgtggat ggatgcgcag
     1861 tttgcctcgc tgaaggcatt gatcgtgaga atggtgtccg gcagcagcga tgctgcggtg
     1921 gctgatttca gccttttacc ggaagagaac gggataccgg agcgaacgga cgaagaactg
     1981 atgcatcttg gggaaggtat ttccggaggt gtgcgttatg gaccagatag ccaacctggt
     2041 cattgatttg gggattgatg cggcagagtt taaaaatgaa attccccgta tcaaaaacct
     2101 tctgaatggt gcagccagcg atgcagaacg gtcttctgcc cgtatgcagc gttttatgga
     2161 gcgtcagact caggcggccc ggcagacaat gcaggcggct tcttcggctg caacagcagc
     2221 atcagcccat gcgcagacgg tggagaagaa tgcacgggct catgaacgca tggcccgtga
     2281 ggtggaacaa acccgtctgc gcgtggatgc cctgaatcag aaaatgcgcg aggaacaggc
     2341 gcaggccagg gcggctcagc tgggggtaag cagtgctgca gaagtgtata tccgcaaaat
     2401 ggagcgggca ggaaaagcca cacattcgct ggggctgaaa agtgcggcag cccggcggga
     2461 gctgggggtg ttaatcagtc agatggcgcg cggcaatttt ggtgcgctga ggggatccgg
     2521 gataacgctg gctaaccgtg ccggatggac aggcgcactg atgtcgccga aaggcatgat
     2581 gactggcggc gttattggcg gacttgtcgc ggcggtcctg ggtctgggta aagcctggca
     2641 tgacggccgg aaggagggcg agaaatttaa ccgtcagctg gcgctgacgg gacattatgc
     2701 cggtgtcact gtcgggcagt tgtggaaact cagccgggcc atatccggga atggtatcac
     2761 gcaacatgcg gcagccggtg cgctggcaca ggtggtgggc agtggggcat ttcatggaaa
     2821 cgatattggt atggtggcga aagctgccgc acagatggag cgatcggttg gccagtcggt
     2881 cagcgatacc ataaatcagt ttaagcggct gaaggatgat cctgtaaatg ccgcgaaggc
     2941 gctggacaat gcgctgcatt ttttgaatgc cactcagctt gagcagatac gcgtccttgg
     3001 ggagcagggg cggtccagtg atgcggcccg gatcgccatg tctgcgctgg cagaggaaac
     3061 cggtaaacgc acatctgata ttgataataa tctcaatgcg ctgggtagta cgctgcaaac
     3121 cttgtctgac tggtggaagc agttctggga tgcggccatg aatattggtc gtgaagactc
     3181 gctggatgcg cagattgatg cgttacagga aaaaattcag cgcgcgaaaa aatatccgtg
     3241 gacaaacgcc tccacacagg tggagtacga tcagcagcgt cttaacgatc ttcaggagaa
     3301 aaaacgccgg aaggatttgc aggatgcaaa agcgcaggca gaacggaatt accaggagca
     3361 acagaaacgc cggaatgctg aaaatgccgc gctgaaccgg atgaatgaaa cggaagctgc
     3421 acgacatcag cgggaaattg cgcgtattaa tgccatgcag tacgccgacc aggctgtcag
     3481 ggatgcggcg atacagcgtg aaaatgaacg ttacgaaaaa gccattaaga aaaatacacg
     3541 ggcaacccgt aatgatgagg ccacccggtt attgctgcag tacagtcagc agcaggcaca
     3601 ggtggaaggg cagattgctg ctgccagaca gtcagcaggc attgccactg aaaggatgac
     3661 tgaagcgcat aaacagcttc tggccctgca gcagcgcatc agcgacctgg acgggaaaaa
     3721 gctgacggca gatgaaaaga gtgtgctggc ccgtaagaat gagctgattc aggcgctgac
     3781 gctgctggat gtgaaacagc aggagctgca gaaacagaca gcgcttaacg acctgagaaa
     3841 aaaaacggtt cagctgacca gccagctggc agacaaagaa cgtgcactgc gtgagcagca
     3901 caatctggat attgccactg caggtatggg ggataagcag cggcagcgct accaggcaca
     3961 gttgcgcatc cggcaggaat accggcaaca gttgcaacag cttgagaatg acagtcgcca
     4021 gaaaggcact tacgggacgg aggactaccg gagggctgag gaggtgctga aggggagcct
     4081 gaagcgacaa ctgaatgaaa acaaacgcta ctggcaggaa ctggaagtgg cgcagggcga
     4141 ctggaaaaac ggtgccatgc gggcgtttca gaattttacg gcggatgcgg ataatgcggc
     4201 gggaacggca gaacagatgt ttacagtggc attcagcagt gccggtaatg cactggcgac
     4261 attctgtacc accggtaagc tgaattttaa atccttcacc tcttccctgt tgtcagatat
     4321 ggccagaatt atggcacaga tggccatgat gcaggcggta aagggcgtcg gttctttatt
     4381 cggcttcacg actaatgctg atggcggtgt ttaccagtct gctgatttga gtcgctacag
     4441 tggcacggtg gttaaccgtc cgacgttttt tgcttttgca aaaggcgcgg gtgtgatggg
     4501 ggaagcggga cctgaagcca ttctgccact gcgtcgcggt gctgatggta agctgggggt
     4561 tgtggcggat actggtggtt caggcatggt gatgtttgcc ccgcagtaca acattgagat
     4621 caataacgac ggcacgaacg ggcagatagg tccggctgcc ctgaaggtgg tttatgacct
     4681 cgggaaaaaa gcggccgcgg actttatgca acagcaggcc cgtgatggtg gccggttaag
     4741 tggagcatac cggtaatgga gacgtttcac tggaaagtgc gcccggatat gaatgtggta
     4801 tcagagccga aagtggtgac agtgaagctg ggcgatggtt atgaacagcg tcgtgcggcg
     4861 ggactgaata accagttgtc gacttacagc gtgacgatac gtgttcgtaa aggtgaacac
     4921 ccatctttaa aagcctttct ggaacggcac ggtggcgtcc gcgcatttca gtggacgcca
     4981 ccttatgact ggaaactgat cagggtggtt tgtcgtaaat ggtcggcaag cgtgggggcg
     5041 ttatgggtga ctgtaacggc cgattttgaa caggtggtga actgatgcag gatatccggc
     5101 aggaaacact gaatgaatgc acccgtgcgg agcagtcagc ccgggtggag ctctgggaaa
     5161 tcgatctgac agaggtcggt ggtgagcgtt attttttctg taatgagcag aacgaaaaag
     5221 gtgagccggt cacctggcag gggcggcagt atcaggcata ccccattcag gggtcggggt
     5281 ttgaactgaa cggcaggggc tgtgctgccc gtccgacact gacggtctct aacctgcacg
     5341 gcatggtcac cgggatggcg gaagatctgc agagtctggt cggcggaacg gtggtccggc
     5401 gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt caacggaaac agcgacgccg
     5461 atccggaaca ggaggtgatc agccgctggc gcatcgagca gtgcagcgaa ctgagcgcgg
     5521 tcagtgcctc ttttgtgctg gccacaccaa cggagacgga tggcgcggtt ttcccggggc
     5581 gtatcatgct ggcgaatacc tgtatgtgga cttaccgttc tgatgagtgt ggttacacgg
     5641 gcagggcagt ggctgacgag ttcgacaaac caacaacgga tatccggaag gacaaatgca
     5701 gcaagtgtat gcgcgggtgt gagttgcgca acaataccgg taatttcggc ggtttccttt
     5761 ccatcaataa actttctcag taaatccatg acacagacag aatcagcgat tctggcgcac
     5821 gcccggcgat gtgcgccagc ggagtcgtgt ggcttcgtgg tgagaacgcc ggagggagac
     5881 aggtatcttc ccagcgagaa tatctccggt gagccggagg aacggttccg gatggctccg
     5941 gaggactggc tgcgggcaca aatgcagggt gagattgtgg cactggtcca cagtcatccc
     6001 ggtggtctgc cctggctgag tgaggctgac cggcggctgc aggtgcagag tgatttgccg
     6061 tggtggctgg tctgccgggg ggcgattcac aagttccgct gtgtgccgca tctcaccggg
     6121 cggcgctttg agcacggggt gacggactgt tacacgctgt tccgggatgc ttatcatctg
     6181 gcggggattg agatgccgga ttttcatcgc ggggatgact ggtggcgtca cggtcagaat
     6241 ctctatctgg ataatctgga ggccacaggg ctgtatcagg tgccgttgtc atcagcacaa
     6301 ccgggcgatg tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac
     6361 tgcggcgatg gtgagctgct gcaccatatt cctgaacaac tgagtaaacg agagaggtat
     6421 accgacaaat ggcagcgacg cacacactcc ctctggcgtc accgggaatg gcacgcatct
     6481 gcctttacgg ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg aaaacggggg
     6541 ctgaagccat ccgcgcactg gccacacagc tcccggcgtt tcgtcagaaa ctgagtgacg
     6601 gctggtatca ggtgcgcatt gccgggcgtg atgcaggtga aaccgaattg tctgcccgtc
     6661 ttaatgagcc gctggcaaat ggtgcagtga tccacatagt accgcgtctg gtgggagcta
     6721 aaagtggcgg tgtgtttcag gcggtgctgg gggcagctgt tatggcggtt gctatatgga
     6781 tgccgggggt aggaattatg gcgagtaatc tgctgttttc tctcggtgcc agtatgacgc
     6841 ttggcggtgt tgcacagatg ctggcaccga aagccagaac tccccgtaca cagacaacgg
     6901 ataacggcaa acagaacacc tgtttctcct cactggataa catggttgcc cagggcaatg
     6961 ttctgcctgt tctgtacggt gaaatgcgcg tggggtcgcg ggtggtatct caggagatca
     7021 gcacggcaga cgaaggggat ggtggtcagg ttgtggtgat tggtcgctga tgcaaaatgt
     7081 ttcatgtgaa accgcctgcg ggcggttttg tcgtttatgg agcgtgagga atgggtaaag
     7141 gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa cctgaaatcc acgcagttgc
     7201 tgagtgtgat tgatgccatc agcgaagggc cggttgacgg tccggtggat ggattaaaaa
     7261 gcgtgctgct gaacggtacg ccggtcctgg atagcgaggg gaagacaaac ttttccggtg
     7321 ttacggtggt gttccgcgcc ggcgagcagg agcagacacc gccggagggg tttgaatctt
     7381 ccggctcaga gactgtgctg ggtacggaag tgaaatacga cacgccgatc acccggacca
     7441 tcacgtcggc aaacattgat cgtctgcgcc tgaccttcgg tgtgcaggca ctggtggaaa
     7501 ccacctcaaa gggggaccgg aatccgtcgg aagtccgcct gctggttcag atacagcgta
     7561 acggtggctg ggtgacggaa aaagacatca ccattaaggg caaaaccact tcgcagtatc
     7621 tggcctcggt ggtggtgggt aacctgccgc cgcgcccgtt taatatccgg atgcgcagga
     7681 tgacaccgga cagcaccaca gaccagctgc agaacaaaac gctctggtcg tcgtacaccg
     7741 aaatcatcga tgtgaaacag tgctacccga acacggcgct ggtcggcgtg caggtggatt
     7801 cagagcagtt cggtaaccag caggtgagtc gcaattatca tcttcgcggg cgcattctgc
     7861 aggtgccgtc gaactataac ccgcagacgc ggcaatacag cggtatctgg gacggaacgc
     7921 ttaagccagc atacagcaac aacatggcct ggtgtctgtg ggacatgctc actcatccgc
     7981 gctacggcat ggggaaacgt cttggtgcgg cagatgtgga caaatgggcg ctgtatgtca
     8041 tcggccagta ctgcgaccag tcagtgccgg acggatttgg cggcacggag ccgcgcatca
     8101 cctgtaacgc ttacctgacc acacagcgta aggcgtggga tgttctcagt gatttctgct
     8161 cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct gacgttcgtg caggaccgac
     8221 cgtcggataa agtgtggacc tataaccgca gtaatgtggt gatgccggat gatggcgcgc
     8281 cgttccgcta cagcttcagc gccctgaagg accgccataa tgtcgttgag gtgaactgga
     8341 ttgatccgga caacggtcat gagacggcga cagagcttgt ggaggacacg caggccattg
     8401 tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg ctgtaccagc cggggacagg
     8461 cgcaccgcgc cgggctgtgg ctgattaaaa cggaactgct ggaaacgcag accgtggact
     8521 tcagcgtcgg cgcagaaggg cttcgccatg taccgggtga tgtcattgaa atctgcgatg
     8581 atgattatgc cggtatcagc accggtggtc gcgtactggc ggtgaacagc cagacccgga
     8641 cgctgacgct cgaccgtgaa atcacgctgc cgtcctccgg taccacgctg ataagcctgg
     8701 ttgacggaaa tggcaatccg gtcagcgtgg aggttcagtc cgtcaccgac ggcgtgaagg
     8761 tgaaagtgag ccgggttcct gacggcgttg cagaatacag cgtgtggggg ctgaagctgc
     8821 cgacgctgcg ccagcgcctg ttccgctgcg tgagtatccg tgagaacgac gacggcacgt
     8881 atgccatcac cgccgtgcag catgtgccgg aaaaagaggc catcgtggat aacggggcac
     8941 actttgacgg tgaccggcgc ggcacggtga atggtgtcac gccgccagcg gtgcagcatc
     9001 tgaccgccga agtcaccgca gacagcgggg aatatcaggt gctggcgcga tgggacacgc
     9061 cgaaggtggt gaagggtgtg agcttcctgc ttcgcctgac cgtggcagcg gacgacggca
     9121 gtgagcggct ggtcagcaca gccaggacga cggaaaccac ataccgcttc aggcagctgg
     9181 cgctggggaa ttacagtctg acagtccggg cggtaaatgc ccgggggcag cagggcgatc
     9241 cggcgtcggt atcgttccgg attgcggcac cggcagcgcc tgtcactatt gaactgatac
     9301 cggggtattt tcagataacg gtggtcccga aactggctgt atatgacccg acggtgcagt
     9361 ttgagttctg gttttcggaa aagcggattg cggatatcag gcaggttgaa accagcgccc
     9421 gctatcttgg cacggcgctg tactggatag ccgccagtat caatatcagg ccgggccatg
     9481 attattattt ttacgttcgc agtgtgaaca ccgttggcaa atcggcattt gtggaggccg
     9541 tcggtcgggc gagcgatgat gcggaaggtt atctgagttt ttataaaggg ttgatcaata
     9601 aaacgcatct cggcaaggag ttgtggacgc agattgataa cggtcagctt gcgccggacc
     9661 tgactgaaat caggacgtcc attacgaatg tcagcaatga aatcacgcaa accgtcaata
     9721 aaaaactgga aaatcagagt gctgcaatcc agcagataca gaaagttcag gttgatacaa
     9781 ataataacct gaacagcatg tgggctgtga agctgcaaca gatgaaggac ggacgccttt
     9841 atattgcgtg gtatcggtgc cggtattgag aatacgccag caggaatgca gagtcaggtg
     9901 ctgctggcgg cagacaggat tgcgatgatt aatcctgcga atggcaacac aaagccgatg
     9961 tttgttggtc agggcgatca gatattcatg aacgacgtgt tcctgaaacg cctgacggct
    10021 ccgaccatta ccagcggcgg taatcctccg gcattttccc tgacacctgg cggacggctg
    10081 acggcgaaaa atgccgatat cagcggtaac gtgaacgcga actccgggac gctcaacaac
    10141 gtcacgatta ataagaactg tcgggttctg ggaaaattgt ccgcgaacca gattgaaggc
    10201 gatctcgtta aaacagtggg caaacctttc ccacgggact cccgggcacc ggagaggtgg
    10261 ccatcaggga ccattaccgt cagggtttat gacgatcagc cgtttgatcg gcaaattgtt
    10321 attcccgcgg tggcgtttcg cggtgctaaa catgagcgga agaataacaa tatttattcg
    10381 tcatgccgcc tgatagtgaa gaaaaacggt gctgaaattt ataaccgaac gaccctggat
    10441 aatacgctga tatatacggg tgttattgat atgcctgccg gtcacggtca catgacgctg
    10501 gagttttctg tatcggcatg gctggtaaat ggctggtatc ccacagcaag tatcagcgat
    10561 ttgctggttg ttgtgatgaa gaaatccaca gcaggtatca gtatcagctg aattttataa
    10621 cccagatacg ggcaccagaa atggtgcctt ttttattgca gaaaagcgag aggtaattat
    10681 gcgtaaactt tatgctgcca ttttgtccgc agccatctgt ctggctgtat ccggtgcgcc
    10741 tgcatgggcg tctgaacatc agtccacgct gagcgcgggg tatcttcatg cctcgacgaa
    10801 cgctcccggc agtgatgatc tgaacgggat taacgtgaaa taccgttatg agtttacgga
    10861 cacgctgggg ctggtgacgt cattcagcta tgccaacgct gaagatgagc aaaaaacgca
    10921 ttacagtgat acccgctggc atgaggattc cgtgcgtaac cgctggttca gcgtgatggc
    10981 ggggctgtct gtgcgcgtga atgaatggtt cagcgcgtat gcgatggcgg gcgtggctta
    11041 cagccgtgtg tcgaccttct ccggggatta tctccgcgta actgacaaca aggggaaaac
    11101 gcacgatgtg ctgaccggga gtgatgacaa ccgccacagc aacacgtctc tggcgtgggg
    11161 ggctggcgtg cagtttaacc cgaccgaatc cgtggccatt gaccttgctt atgaaggttc
    11221 cggcagtggc gactggcgca ctgacggttt catcgtgggt gtcggttata agttctgatt
    11281 agccaggtaa cacagtgtta tgacagcccg ctggttcagg cgggcttttt gtggggtgaa
    11341 tatggcagta aagatttcag gtgtactgaa agatggggcg ggtaaacctg tcgtaaattg
    11401 tgcgattgaa ctgcgggcca gaagaaccag tccgaccgtt gtggcacacg ttgttgccac
    11461 ttgcgtgacg gacaataacg gtgcttatgt gattgaggct gagccggggt attacgaggt
    11521 tgcgcttcac tgtaacggct ggcagccaac ccgtgtcggg gatattgatg tggcaccgac
    11581 tgatgcaccg gggacactga acgcgtttct gaatgcacca aaggatggtg atttacgtcc
    11641 ggaggtgatg aagcgctttg aggaaatggt ggcgcaggcg cagcagagtg ccggggctgc
    11701 agccggaaac gcacagcaga cggcgcagga tgtggcggca gccgcaacgg cccgtgatga
    11761 tgcacaacgt tttgcggaga aagcccgaca ggatgcaacc gtcacagctg aggacagaaa
    11821 ggccactgcg gaagatgtga caagcacagg agcaaatgca gccgcagccg gacagagcgc
    11881 acaggatgcc gcaggttatg cccgcgcagc agaacaggcc aaaaatgaca ttgatgctgc
    11941 gctgaccggc actctgaaaa tggctaacca tctgtc
//
LOCUS       DAEEGA010000107        11924 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEEGA010000107 DAEEGA010000000
VERSION     DAEEGA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.107"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..2341)
                     /locus_tag="KLH46_003415"
     CDS             complement(<1..2341)
                     /locus_tag="KLH46_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBE0379982.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="KLH46_003416"
     CDS             complement(2275..2898)
                     /locus_tag="KLH46_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBE0379983.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="KLH46_003417"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="KLH46_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="KLH46_003418"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="KLH46_003418"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBE0379984.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /locus_tag="KLH46_003419"
     CDS             7506..8228
                     /locus_tag="KLH46_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBE0379985.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..10287)
                     /gene="yfaL"
                     /locus_tag="KLH46_003420"
     CDS             complement(8356..10287)
                     /gene="yfaL"
                     /locus_tag="KLH46_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBE0379986.1"
                     /translation="MEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMAD
                     SSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDG
                     ATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDG
                     VTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTL
                     LFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQN
                     NAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPT
                     PDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYT
                     AAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQ
                     NHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEV
                     GYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAV
                     GVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQ
                     KGSDDFAQTAGFLSMTVKW"
     gene            complement(10300..11895)
                     /locus_tag="KLH46_003421"
     CDS             complement(10300..11895)
                     /locus_tag="KLH46_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HBE0379987.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2758 a         3307 c         2946 g         2913 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagaccgt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tcaggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccgacttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag ctatccagcc aggccccctg
     8881 cttctgatta ccgtgctgat accagcttga ggtcagccca acggcataac cgtggttctg
     8941 gttatcggcg cgagttccgg tcatattcga gcggctgtcg ccctgattat cactgtagcc
     9001 accaaccgcc ccaagcatcc actcgccatc cgtgccccag cgcccgctaa acaagccgcc
     9061 gctaagctgc accgtagaag tgtcttcctg ctgagccagt tgccctgccg ccgtgtaatg
     9121 ataacgtccg ccgataacac gtaaattcag cgtctgacca tcgccaccag cgtgatcgcg
     9181 tcgctccatc ataaacgcct gatttgccgc ccgcaggtta ttgagataac cgccaacttt
     9241 ggcattcagc accggctggt aagcaggcgt aggggccggg tcgggggttg ggtcaggatc
     9301 cggcgtggga tcaggatcgg gagtcgggtc tggctcaggt ggcgatggcg gcgttacttc
     9361 ttgcgatcgc agataccagt cgttgttatc ttccactagc gtgtagtcat acgctcccat
     9421 gttgacgtag ccactgccag ccagactgaa ctgtgcattg ttttgaaact gcgtgggatc
     9481 agctgcgaaa tcaaccactt taatgcctgt cgatgtcggc tcaccaatcc ctgtaatgga
     9541 gttaaccacc acagttgtgt tgccagcagt attaccgttc atcaccaatt gatcgcttac
     9601 cgagtcatcg ccgtttaatt cgctgtcgaa tagtagcgta ccgccgccgg tataatcacc
     9661 gtttaccgtc agcgtatcgc cagcgacgcc attttgcagg ctaaccgcac cggacgtatt
     9721 ggtcagatca ccattaaccg tcacgtcagc aagataggtc aggctgtcag tttcaaggtt
     9781 ggtattaagc tcgcctgtca acgtcacgcc atcggtagca tttaccagcg tcccatcgcc
     9841 gttaaatagt gaggcattaa gggcgacgga agtatcctgc ccggtcagac gcaaaaccgt
     9901 accatcgctg atgtcgatag tgccgctgga agtagcatca attgactgaa tatcctgatc
     9961 cgcgccagta acgaacgttg ccccatcacc aacccacagt gacgaagcat aaggaaagat
    10021 atccgcaaca tcgcctttca gggtgccttc ttcgacacgt accgccgact gcgttgtacc
    10081 gctggcggtc agctccagcg tacccgcccc cgttttagta aatgtgctac cctcatcctg
    10141 atgctgcccg ctgctgtcag ccatcagtgc gccccactgc gtgtctaccc ccgcatcaac
    10201 tgccacttca ccgtcggcgc gcatttcaat atcacgccca tgctcagtcg tcgtagagcg
    10261 agtatcgaca ttggcagtaa tctccatcac gctttcttgt tacttatcgg taaagataac
    10321 ctggcggtta tagtgggtat ctccaagctg cgagttgtcg ctcaccatca gcgtaccgga
    10381 ggcgatttgt gttgtgccca aataactatt attgtttgcc agacttacct gcccgtcgct
    10441 cgcatcgacc accacgtaga cgccatcttt cccgtcgccg ccgatatgca aattgtcgcc
    10501 acctgcaaga tctaccacat cctgactgcc gataaccgtt ccgctgctgc cactgacttc
    10561 aaggccatca ttgtatgatg tgccgctctg ccaagtggag aaatcagaga gatcgagcac
    10621 accgccgttt aacacgatcg actgcggatc gtcctggaga gcggtaagat ctgccgcgtc
    10681 gccttccagc gaaagcaccg caccatcatc aacgactata tcgccggtta gcgccatcga
    10741 ctgcgcccct gccagcacgt agctgccgtt ttgcgcaatg gtgagctgac cagcaccttc
    10801 gatgatgcca gcaaaactgc cctgattaac agtaacgttg ccaccagcat cgatatttaa
    10861 agtgccattc tgaaagcccg tcaatgcgtg cacaaaagtt tgttgggtcg agccaacgtt
    10921 tagctcagcc tgattctgat actgatcaat actccctatc gtcagaccgt agcagtcttg
    10981 cggatcgtcc tggcaatgcg tatcgccgac attcatcagg gagttgctgc gacccagggt
    11041 aacttcaccg ttttcaatct gcatctcacc agtaaagtca ttgttatctg cattaagtac
    11101 cagatcgccg gaacctgttt tggtgattaa cccggtacca gcaatagagt caacagctcc
    11161 gtcattctct gtattgccga taaccagcgt ttttccgtcg gcaatatcaa aggtaacttc
    11221 acttaagccg agatacataa agccacccgc cgcagtggag ggaccatctc catagcctgc
    11281 tgcgctattg ttctcatcga ctaacacgcc gccgttctgg ctgtagctgt catcaacaga
    11341 aatatcaata agatagggag ccgtcgcgct attggtatat atcgccccgc cataaccttc
    11401 ggctgtgtta tttgtaaagg cggtgttatt tattatcgta taacctgaag gatgcttgct
    11461 gtcgctatta ttatcggtaa catcgattgc cccgccatcg ccatcactgt aacttgttga
    11521 tgtatatgcc tggttattgt taaaaacatc atcacttaaa tagacgtcat tattaatggt
    11581 ataaattgcg ccacctttgc catcattagc gatattattg atggcgttag tgacacgtaa
    11641 atcgacggca ccagtatcat tagtaccaga agaatagatt gcgccaccat agccgcctgc
    11701 gacgttaccg gaaaaaataa catcagtaag attcagcgtt gagttttctt tagcaaatat
    11761 ggccccgccg ttattatatt ctcctgtaac ggtgttattg gcaaacagag tcattccagt
    11821 ttcattttct ggtaatagtg aaaattccgc tccttgttgc aaaaatacgg ccccaccgct
    11881 ggcattattg gtcatatccg aaaaaaccag ccattgccca tcgg
//
LOCUS       DAEEGA010000108        11668 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEEGA010000108 DAEEGA010000000
VERSION     DAEEGA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.108"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            597..794
                     /locus_tag="KLH46_003422"
     CDS             597..794
                     /locus_tag="KLH46_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379988.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="KLH46_003423"
     CDS             complement(744..1937)
                     /locus_tag="KLH46_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0379989.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="KLH46_003424"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="KLH46_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HBE0379990.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="KLH46_003425"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="KLH46_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HBE0379991.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="KLH46_003426"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="KLH46_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HBE0379992.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="KLH46_003427"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="KLH46_003427"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HBE0379993.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="KLH46_003428"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="KLH46_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HBE0379994.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="KLH46_003429"
     CDS             complement(10573..10716)
                     /locus_tag="KLH46_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379995.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10966..>11668
                     /locus_tag="KLH46_003430"
     CDS             10966..>11668
                     /locus_tag="KLH46_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE0379996.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIRGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWD"
BASE COUNT         2961 a         2899 c         2993 g         2815 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgctcagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctgccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattaa
    10801 cgatcaatta gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt
    10861 tacaatatag aactgttact tttagcaagc tgttagtaac caaccgttac taataatttt
    10921 aattcttaat actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac
    10981 tcccttaaat atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga
    11041 gtttctggta aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt
    11101 acagtaaatg cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt
    11161 gcacaaaata aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac
    11221 ggagataaat tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt
    11281 ggcgcagcta ccgcaataag aggttcttat agtgttactg ttgcacataa caaaaagaac
    11341 cctcaggccg cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga
    11401 aattccaatg attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc
    11461 accccggcag agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc
    11521 acttctgacg gttcaaaaaa aatcataggt tttcgtgctg gctctggagg aacatcattt
    11581 attaatggtg aatccaaaat ctcaacaaat tcagcatata gccatgacct gttaagtgct
    11641 agtctatttg aggtcaccca atgggact
//
LOCUS       DAEEGA010000109        11631 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEEGA010000109 DAEEGA010000000
VERSION     DAEEGA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.109"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(282..357)
                     /locus_tag="KLH46_003431"
     tRNA            complement(282..357)
                     /locus_tag="KLH46_003431"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
     gene            916..3168
                     /locus_tag="KLH46_003432"
     CDS             916..3168
                     /locus_tag="KLH46_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0379997.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3504..4481
                     /gene="csiD"
                     /locus_tag="KLH46_003433"
     CDS             3504..4481
                     /gene="csiD"
                     /locus_tag="KLH46_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBE0379998.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4501..5769
                     /gene="lhgO"
                     /locus_tag="KLH46_003434"
     CDS             4501..5769
                     /gene="lhgO"
                     /locus_tag="KLH46_003434"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBE0379999.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5792..7240
                     /gene="gabD"
                     /locus_tag="KLH46_003435"
                     /pseudo
     CDS             5792..7240
                     /gene="gabD"
                     /locus_tag="KLH46_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7248..8534
                     /gene="gabT"
                     /locus_tag="KLH46_003436"
     CDS             7248..8534
                     /gene="gabT"
                     /locus_tag="KLH46_003436"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBE0380000.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8772..10172
                     /gene="gabP"
                     /locus_tag="KLH46_003437"
     CDS             8772..10172
                     /gene="gabP"
                     /locus_tag="KLH46_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBE0380001.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVISFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10193..10855
                     /gene="csiR"
                     /locus_tag="KLH46_003438"
     CDS             10193..10855
                     /gene="csiR"
                     /locus_tag="KLH46_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBE0380002.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10856..11305)
                     /gene="kbp"
                     /locus_tag="KLH46_003439"
     CDS             complement(10856..11305)
                     /gene="kbp"
                     /locus_tag="KLH46_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBE0380003.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11389..11547)
                     /locus_tag="KLH46_003440"
     CDS             complement(11389..11547)
                     /locus_tag="KLH46_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBE0380004.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2992 a         2855 c         2900 g         2884 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 cccacattgt tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta
     1021 gaatatctaa actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta
     1081 aaagacagag aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa
     1141 aacgattata aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca
     1201 cagagtagca ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga
     1261 gatacaatgg ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat
     1321 ttcactcaca ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt
     1381 aacattcctg actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc
     1441 ggacagcaag aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag
     1501 cgtaaatctc ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct
     1561 ggcgaagcga ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct
     1621 gagtttgcac gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc
     1681 gacggaccag aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa
     1741 gatctttgta ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa
     1801 gatgctaaaa aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc
     1861 ggggggagtg gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt
     1921 aaagatatag aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt
     1981 aaagatccag taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca
     2041 aaagacttcg aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca
     2101 ttaaaagaaa agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt
     2161 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
     2221 gatgcagcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
     2281 aattataatg agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc
     2341 gttatgaatt acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg
     2401 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata
     2461 aaagcctttt cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc
     2521 tcaagcatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     2581 ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     2641 tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa
     2701 tcaacgactg aaattaaaaa ttatgatggg tccacgcgtg tcggtggtga ggcgtggctt
     2761 aataaaggtt gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat
     2821 aaagcaggaa tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt
     2881 agtcgtgggg attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat
     2941 aaaggaaacg aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa
     3001 aacatgggga gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct
     3061 tgtaaagtat ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct
     3121 cttcttttag ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata
     3181 tctgccccga taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg
     3241 aagccattta ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa
     3301 cctgttgctt tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt
     3361 tactaatttg ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt
     3421 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
     3481 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg
     3541 attcaggcca ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc
     3601 tggaactcac cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc
     3661 ccgtgcaggc gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg
     3721 atctgtgtgc gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag
     3781 gtgcgctgtt gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga
     3841 agctggcaac ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc
     3901 agtattacgc gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc
     3961 cgcaccgcgt gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg
     4021 tgctgatgat gaaaatcgat gagcaaaaca tgcagggtgg aaattcgttg ctgctgcatc
     4081 tcgatgactg ggaacatctg gaccactatt tccgccatcc actggcgcgt cgcccgatgc
     4141 gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt tttccatcca gtgttcgacg
     4201 ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag ccaaaagatt
     4261 ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc aaaggcattc
     4321 tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa caacctgttc tggctgcacg
     4381 gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt cagcgtggct
     4441 atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg aattgagcgg
     4501 atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc catgcaactg
     4561 attgatgttt atccggatgc ccgcattgcg ttgctggaaa aagagtccgg cccggcctgt
     4621 caccagacgg gccacaacag cggcgtgatc catgccgggg tctattacac gcccggcagc
     4681 ctgaaggcgc agttttgcct ggcggggaac cgtgccacca aagccttttg cgatcaaaac
     4741 ggcattcgct atgacaactg cggcaagatg ctggtcgcca cgtctcagct cgaaatggaa
     4801 cggatgcgcg ccttatggga acgcacagcg gcgaacggta tcgagcgcga gtggataaac
     4861 gccgtggaac tgcgcgagcg cgaaccgaat atcaccgggc ttggtggcat ttttgtgccg
     4921 tccagcggca ttgtcagcta ccgcgaagtg acggcggcaa tggcaaaaat cttccaggcc
     4981 agaggcggcg agattattta taacgccgaa gtcagcgccc tcagtgagca taaaaacggc
     5041 gtggtgatac gcacccgtca gggcggtgaa tatgaagcat cgacgctgat tagctgttcc
     5101 gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt tattatctgc
     5161 ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt taaccacctg
     5221 atttacccca ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac ccgcatgatc
     5281 gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga aggctatcgc
     5341 aagcgcgact tctcatttag tgacacgctg gagattttga gctcgtcggg gattcgccgg
     5401 gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct gtgcaaaagc
     5461 ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga tctccagccc
     5521 tggcccgccg gtgtgcgggc gcaggcagta tcgccggacg gcaagctgat tgacgatttt
     5581 ctgtttgtca ccaccccacg cacgatccac acctgcaatg cgccctcccc ggcagcgaca
     5641 tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt ggcaagccag
     5701 agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca cgccgcattt
     5761 aatcaataac ctttgaaaac aggacgtaac aatgaaactt aacgacagta acttattccg
     5821 ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggtgaag tcatcgacgt
     5881 caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg cagatgaaac
     5941 ccgcgccgct atcgacgccg ccaaccgcgc cctgcccgcc tggcgtgcgc tcaccgccaa
     6001 agaacgcgcc accattctgc gcaactggtt caatttgatg atgcagcatc aggacgattt
     6061 agcgcgcctg atgaccctcg aacagggtaa accgctggct gaagcgaaag gcgaaatcag
     6121 ctacgccgcc tccttcattg agtggtttgc tgaagaaggc aaacgtattt atggctacac
     6181 cattcctggt catcaggccg ataagcgcct gattgttatc aagcagccga ttggcgtcac
     6241 cgccgccatc acgccgtgga actttccggc ggcgatgatt acccgtaaag ccggtccggc
     6301 gctggcggca ggctgcacca tggtgctgaa gcccgccagt cagacgccgt tctctgcgct
     6361 ggcgctggcg gaactggcga ttcgcgcggg cattccggct ggcgtgttta acgtggtcac
     6421 cggttcggca ggcgcggtcg gtaatgaact gaccagcaac ccgctggtac gcaaactgtc
     6481 gtttaccggt tagaccgaga ttggccgcca gttaatggaa cagtgcgcga aagacatcaa
     6541 aaaagtgtcg ctggagctgg gcggcaacgc gccgtttatc gtctttgacg atgccgatct
     6601 cgataaagcc gtggaaggcg cgctggcctc gaaattccgc aacgccgggc aaacctgcgt
     6661 ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg aaaaattaca
     6721 gcaggcggtg agcaaactgc acatcggcga cgggctggag aaaggcgtca ccatcgggcc
     6781 gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg cgctggagaa
     6841 aggtgcgcgt gtggtttgcg gcggtaaagc gcacgaacgc ggcggcaact tcttccagcc
     6901 aaccattctg gtggacgttc cggccaacgc caaagtgtcg aaagaagaga cgttcggccc
     6961 cctcgcccca ctgttccgtt ttaaagatga agccgatgtg attgcacaag ccaatgacac
     7021 cgagtttggt cttgccgcct atttctacgc ccgtgattta agccgcgtct tccgcgtcgg
     7081 cgaggcgctg gagtacggca tcgtcggcat caataccggc attatttcca atgaagtggc
     7141 cccattcggc ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt atggcatcga
     7201 agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg cgaaggaaca
     7261 gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt gggcaaattc
     7321 acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgttgaa ggccgtgagt
     7381 atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat ccgaaggtgg
     7441 tggccgcggt ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag gtgctggctt
     7501 acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc gatttcgcca
     7561 agaaaacgct gctggttacg accggttccg aagcggtgga aaacgcggta aaaatcgccc
     7621 gcgccgccac caaacgtagc ggcaccatcg cttttagcgg cgagtatcac gggcgcacgc
     7681 attacaccct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg gggctgatgc
     7741 cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc gaggatgacg
     7801 ctatcgccag catccaccgg atcttcaaaa atgatgccgc gccggaagat atcgccgcca
     7861 tcatgattga gccggttcag ggcgaaggcg gtttctacgc cgcgtcgcca gcctttatgc
     7921 agcgtttacg cgctctgtgt gacgagcacg ggatcatgct gattgccgat gaagtgcaga
     7981 gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtg gcgccggatc
     8041 ttaccacctt tgcgaaatcg atcgcaggcg gtttcccgct ggcgggcgtc accgggcgcg
     8101 cggaagtgat ggacgctgtt gcaccgggcg gtctgggcgg cacctatgcc ggtaacccga
     8161 ttgcctgcgt ggctgcgctg gaagtgttga aggtgttcga gcaggaaaat ctgctgcaaa
     8221 aagccaacga tctggggcag aagctaaaag acggattgtt ggcgatagcc gaaaaacacc
     8281 cggagatcgg cgacgtacgc gggctgggat cgatgattgc catcgagctg tttgaagatg
     8341 gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc gcccgcgata
     8401 agggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc cttgtaccac
     8461 tcaccattga agacgctcag atccgtcagg gtctggagat tatcggtcag tgttttgatg
     8521 aggcgaagca gtagcaccgc ccccatgcag gaggcgacgc tgcgcgtctt gtccggccta
     8581 cgagaatcca agtcggataa ggcgtttacg ccgcatccga caatctgtac gtgaacagga
     8641 ataaatctat gtcggccgga taaggcggag ccgctctccg gcaaaaagaa tcaataacaa
     8701 ttaaacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac acactttcga
     8761 gaggattcag gatggggcaa tcatcgcaac cacacgagtt aggcggcggg ctgaaatcac
     8821 gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt gtcggttcca
     8881 gcgtcgccat cgccgaagcg gacccggcgg tattactggc ctatctgttc gccggattac
     8941 tggtggttat gattatgcgg atgctggcgg aaatggcagt tgctacgccc gataccggtt
     9001 cgttttccac ctatgccgat aaagccattg gccgctgggc gggctatacc atcggctggt
     9061 tgtactggtg gttttgggta ctggttatcc cactggaagc caacatcgcc gccatgatcc
     9121 tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt accctcgcct
     9181 taactggcag taatttatta agcgttaaaa actacggcga atttgagttc tggctggcgc
     9241 tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca attagcggtt
     9301 tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc ggctttatgc
     9361 ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg tttatgggcg
     9421 cagaaattgt caccattgcc gccgcggaat ccgacatgcc ggaaaaacat attgtccgcg
     9481 ccaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt tttgtcgtcg
     9541 tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat cgctcggttc
     9601 tggaattgct caatattccc catgcgaaat taatcatgga ctgtgtgata ttactttccg
     9661 taaccagttg cctgaactcg gccctgtata ccgcctcaag gatgctctac tccttaagcc
     9721 gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc ccgtacgtgg
     9781 cggtgttact ctccaccggc gcagcattct taacggtggt ggtgaactat tacgcgcctg
     9841 cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg gtttatttag
     9901 tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga agcgaaattc
     9961 gcttgcgaat gtggcttgat ccctggctca cctggctggt catcagcttt attacctttg
    10021 tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc accggcttat
    10081 tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag ctggtattgt
    10141 ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca ccatgaccat
    10201 tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc
    10261 ggatgaaaaa ttacggatga gtttactgac atcgcgttat gcacttggcg ttgggccgtt
    10321 gcaggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg
    10381 gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg cccgcgccaa
    10441 tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat gggaggcaga
    10501 cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca gcgagaaaat
    10561 gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg
    10621 ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gttatcgatt
    10681 tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac acgatcagca
    10741 ccagaccctg actgcggcgg tactgccgcg agatacagcg cgcgccagtg agttaatgcg
    10801 ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact
    10861 cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt
    10921 tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg
    10981 cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg
    11041 ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca acaaggattt
    11101 tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt
    11161 gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct
    11221 tcgcctgatc gtctttatcg tgctgacctg taaccgcatc ccagagtttt tctccggcat
    11281 ctttaacaaa attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag
    11341 tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt aatcgcgggt
    11401 ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt
    11461 aataatgaac gcccaaccga accctttacc gagcagcacg ccgatcggcg gcagaatgat
    11521 ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt
    11581 aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag t
//
LOCUS       DAEEGA010000110        11087 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEEGA010000110 DAEEGA010000000
VERSION     DAEEGA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.110"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            154..504
                     /locus_tag="KLH46_003441"
     CDS             154..504
                     /locus_tag="KLH46_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBE0380005.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="KLH46_003442"
     CDS             557..952
                     /locus_tag="KLH46_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBE0380006.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="KLH46_003443"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="KLH46_003443"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBE0380007.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="KLH46_003444"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="KLH46_003444"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBE0380008.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="KLH46_003445"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="KLH46_003445"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBE0380009.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="KLH46_003446"
     CDS             complement(5323..6423)
                     /locus_tag="KLH46_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBE0380010.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="KLH46_003447"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="KLH46_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBE0380011.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="KLH46_003448"
     CDS             complement(7571..8137)
                     /locus_tag="KLH46_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE0380012.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="KLH46_003449"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="KLH46_003449"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBE0380013.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..10751
                     /locus_tag="KLH46_003450"
     CDS             10263..10751
                     /locus_tag="KLH46_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HBE0380014.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(10869..10937)
                     /locus_tag="KLH46_003451"
                     /pseudo
     CDS             complement(10869..10937)
                     /locus_tag="KLH46_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
BASE COUNT         2702 a         2833 c         2643 g         2909 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctattcg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 tatagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgagtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgccctg aaccgccccg ggtttcctgg agagtgtttt atctgtgaac tcaggctgcc
    10981 agatcatcgt ttccgatgga agcataataa gctttttctg cttctgccgg aggagtatgg
    11041 cccagccttt ccagcaatcg tcgattgtta taccagtcca cccacgt
//
LOCUS       DAEEGA010000111        10925 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEEGA010000111 DAEEGA010000000
VERSION     DAEEGA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.111"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..203
                     /locus_tag="KLH46_003452"
     CDS             <1..203
                     /locus_tag="KLH46_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380015.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="KLH46_003453"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="KLH46_003453"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBE0380016.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2102..2818
                     /locus_tag="KLH46_003454"
     CDS             2102..2818
                     /locus_tag="KLH46_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE0380017.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3274..3349)
                     /locus_tag="KLH46_003455"
     tRNA            complement(3274..3349)
                     /locus_tag="KLH46_003455"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3314..3316),aa:Asn,seq:gtt)
     gene            complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="KLH46_003456"
                     /pseudo
     CDS             complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="KLH46_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5082..5157
                     /locus_tag="KLH46_003457"
     tRNA            5082..5157
                     /locus_tag="KLH46_003457"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5115..5117,aa:Asn,seq:gtt)
     gene            complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="KLH46_003458"
     CDS             complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="KLH46_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBE0380018.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLEFCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6247..7164)
                     /gene="nac"
                     /locus_tag="KLH46_003459"
     CDS             complement(6247..7164)
                     /gene="nac"
                     /locus_tag="KLH46_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBE0380019.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKIIYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7492..7567
                     /locus_tag="KLH46_003460"
     tRNA            7492..7567
                     /locus_tag="KLH46_003460"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7525..7527,aa:Asn,seq:gtt)
     gene            complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="KLH46_003461"
     CDS             complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="KLH46_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBE0380020.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="KLH46_003462"
     CDS             complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="KLH46_003462"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBE0380021.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="KLH46_003463"
     CDS             complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="KLH46_003463"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBE0380022.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10451..>10925)
                     /gene="cobU"
                     /locus_tag="KLH46_003464"
     CDS             complement(10451..>10925)
                     /gene="cobU"
                     /locus_tag="KLH46_003464"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBE0380023.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3035 a         2782 c         2364 g         2744 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat ataagattat
     1981 ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgcc
     2041 gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc
     2101 agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc
     2161 taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag gtgaacccga
     2221 tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc
     2281 aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt
     2341 acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg agacattgac
     2401 ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa
     2461 tatcggagcg gcaggttctg tcagctatat gtttgacaat acgggtgtga ttgtatttaa
     2521 aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg atgttcgtga
     2581 tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc ttcataaagg
     2641 aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat
     2701 tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga
     2761 tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa atctctaatt
     2821 atattttaaa gaaatctgtc tttatggcag atttctttaa tctcatataa ttcttataaa
     2881 aaatataata tttaactcgt catattgctt ataaaaaatc cccgtcccca gagaaataat
     2941 atttattaaa atggcaggtc ttctttttct gattaccgaa gacaaagtgg caattacgta
     3001 tagtttcccg ataaagacgc gacagctacg tcccgcataa ggcatttttc tctttatctt
     3061 tgtacggtac ttcatggaac agaatttttg accttgtgaa tcgtgatatc tgttggggag
     3121 gggcaatttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca gaaatcaaaa
     3181 aacccggcca cttttttaca aagtggccgg gtaaaaataa tttttatttt ttaactgtcc
     3241 tgagtctcat agagatgtct caaaactaaa atttggctcc tctgactgga ctcgaaccag
     3301 tgacatacgg attaacagtc cgccgttcta ccgactgaac tacagaggaa tcgtgagaac
     3361 gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc tgttttttaa atttgaaatc
     3421 gtttgctgaa ataatctgca ttttgtcgtt tattccgaca caactggctt tttttcacac
     3481 ttttgcggct cggatcgagg gtatttccac agccaacgtc cagtaaccat tcgccagtaa
     3541 aacagcacgg cccgcacagc ccagtcggca aacattccca tccagacacc aaccacaccc
     3601 cagccaagca tgattccctg cacataaccg actacaaccc ggcaacccca catgctcaac
     3661 atagaaaccc acatggcgta acgggcatca cgagcaccct taaatccagc gggtagcacc
     3721 catgaggcgg accaaatagg cataaataaa gcatttagct aaatcagaat cacaacgacg
     3781 tgtttaacct gtggatcctg ggtgtaaaac gatgccataa ccccggcaaa gggagccgtt
     3841 agccaggcga tggccgttaa tccaagagtg gacagccaga acacatgccg caactgaatc
     3901 tctgcttgcg ctatctgccc tacccccaac cttcggcctg taatgatcgt agaagcagaa
     3961 ccgagcgcac ttccgggtaa gttgataaga gccgcaattg agaacgcgat aaaatttccg
     4021 gcaataacgc tggtccccat cccggcaacg aacatttggg ttaataaccg accactggta
     4081 aataacactg attcgacact cgctggaata ccaatcccca tgacttccca gataatgcta
     4141 aaattcagcg gtttaaaata gctctttaac gaaatcctta gcgcaggatt aaaaccaatc
     4201 gccagcaccc acaaaattgc aactgcgcca atataacgag aaatggttaa acccaaccca
     4261 gccccgacca ggagaaaagg ccgtaaatca atatgccgct aataataata ttaagaatat
     4321 tcaggctacc gttaatcagt agcggtattt tcgtattccc tgcaccacga agtgccccgc
     4381 taccaataag agtgatggca gcggctggat aactgagtac ggtcagctcc agataagtca
     4441 acgccagtgc tttaacttct gtcgtggcat cacccgcgac gaaatcaata atttgttcgc
     4501 caaaatgatg aattagcgtt gccaacagta cggcaaacaa cgtcatgatc accaatgact
     4561 gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc gagactaaat gctacaacga
     4621 cagtagtacc aagatcgata gctgcaaaaa aagccataat gaccatattg aagctgtccg
     4681 ccaaccccac gccggccatc gcatcttttc ccagccaact gaccagaaaa gtgctcaggc
     4741 cccccatcaa caggacacag gcgttctcca tgaagatagg aacagcaagc ggggttatct
     4801 cgcgctagaa caacactttg tagctcttgc gtttagagtg ccagcgagtg ccgtgaacaa
     4861 cctggcgtaa agcagaggag atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca
     4921 tttcaaatga tgagggagaa tcagcaaagc tgcaaagatt ttcgccaaca aattgtctgc
     4981 aaatgcaaca aactgttgat agaaacggca aacagttggg aaatttaaaa atcgggtttg
     5041 acaaaatatt tttcgccgtt aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg
     5101 tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat
     5161 ttctgaaaag cccgctttta tagcgggctt tttgctatat ctgataatca atttcctctt
     5221 cactgttttc catcacctgg cgcttgatat cctcaactga cagtcctgca ttacaaaatt
     5281 ccagaaagcg ccaaacatag ttacgctgaa gttgtcctcg cttcagtccc aaccagacag
     5341 tattagcatc gaaaagatgc cgcgtatcca ggcggattaa attctcttcc tcttgttcgc
     5401 cgctggattg ttcggcaact aatccgatcc caagcccaag agcaacatag gttttaatga
     5461 catcagaatc ctgtgcactt aatacaatat ctgccggcaa acctttgcgg gtaaatgcgt
     5521 catcagtgcg tgagcgcccc gtaattccct gtcggtaagt aattaacggc cacttcgcta
     5581 ttgattccag cgtcaatggt gtaatttgcg ttaagggatg atcgagtgga acaagcaaac
     5641 tatggtgcca acaaaaccac gggaaggcga cgagctgcgg gtcattactc aaacgctcgc
     5701 tggcgatacc aatatcagct tcgccatttt gcaacaacgt cgcaatttcc tgtggcgaac
     5761 cctggattag ctcgagccga acctccggga aaagttcgcg aaaagcttta atgacctctg
     5821 gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt gagaacacca gacgtatcgt
     5881 tggtaaacag gtctgcaagc cgacgaacat tactggcttc attcagaata cgttctgcaa
     5941 tgaccagtaa tgctttgccc ggttcagtca tccccagcag tcgcttacct cgtcgaacaa
     6001 atatttcgat gccaaattca tcctccagtt cccgaatatg acggctaacg cctgactgtg
     6061 aggtaaaaag catattcgca acctctgtca ggttgtaatc ctgacgtgca gcctcgcgga
     6121 ttatctttag ttgttggaaa ttcacggtaa actccgggca gttcagatat cccgttattg
     6181 ttaaagtcta atgcccggca taacaaataa taaaaacccg catcttattc catcccgata
     6241 taacgcttag ctcaccaatt gccactgcct tttttccatc actggagaac taatcactga
     6301 cattaacaac tctttcactg cctgtgcctg tggtgacaag tttgctctgg cgggtaaatt
     6361 taaggacaaa gagagactca ttgaaggagt ggtaatgcgt gacatccacc catttactgc
     6421 gccacataac gaacgcgcag ccgattcagg taatactgca acgcccatgc cgctggcaat
     6481 cgctgcggta agcgtggcaa tagactcaat ttcaccaatg acttttgccg tgagccgccg
     6541 tagggaaaaa gcctcatcaa cacgaagtct aacagcgctg taatcactgg ggagaaaaag
     6601 gttcatttgc gcaatagcat taacatcaac gttttgcccc gggcaatctt gagttcctac
     6661 caggaaaaga tcttctttca gcaaagcctg actggatacc ccagccacag gggaatgctc
     6721 ataaatcacc gccatatcga gttggtgatt tatcagtttt tcgttaagca ctgcaccact
     6781 attttcatga agatagataa cgacctccgg gaattcagcg cgaaccgcct gtaataaggg
     6841 catggtgatg gatgacgcag cggttcctgg cgcaaagcca atcgagactt gccccgataa
     6901 tgactgacca acgttatgca ccgccagttg ggcctgctca cactgacgta aaatggcccg
     6961 tgcatgggta tagataattt ttccggcgtc tgttggcgta acgccccgtt ttgtgcgaat
     7021 caaaagctgt tgatttaact caccttccag tatggcaacc tgctggctga gcgctggttg
     7081 tgcgatatgc aatacttcag cagcctgggt caggctacca atatctacaa tttttacgaa
     7141 gtatttcagg cgtctgaagt tcatgttgcc tccggttttt aagaatcggc ccaagtgccg
     7201 ccattactta caaccagtat gcaagatgcc tgccagttct attttggcgt tgatgcgcaa
     7261 gctaaccaac tgtcaaataa gagattatga tagattcgtc attggccctt tttatcagca
     7321 gtcgcattca cctgccctat aaaaggaggg tatgcaccac gatggttcat tacccaataa
     7381 gattgaaagc tcaccacttt gttgaaattg gcagcaaaca aacaaaaaaa atgcatttca
     7441 ccctttgaca tcaccatgca ctgccattaa tatgcgcccc gttcacacga ttcctctgta
     7501 gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga
     7561 ggagccaaat tcaaaaaagc ctgctttcga gcaggctttt tactttctaa ttaccaacgc
     7621 tcttaaaaca tctgtcttga accagaacta atttgcacag gcattcccga tcgatgttgc
     7681 aacgcagcat ttgcgcgatt tacatcaact tcttgcccgt tgataaacgc ccgcaaagat
     7741 ggggttaccg gcaatggcac ttttcggtca gactcatatt ctgcacgatt gcgcgacagc
     7801 ggctcatgaa cttccagcca ctttgaacca tccggttccg ttgtgtattt cactggctga
     7861 tcaataattt gtacacgagt gcctacagga acattatcaa acagatattt gatatcgtca
     7921 ttacgcagac gaatacagcc ctgacttaca cggagcccaa taccaaaatt ggcattggta
     7981 ccgtggatgg catacaacct gccaatataa atcgcgtaca gccccatggg gttatcgggc
     8041 cccgcaggaa caaatgcggg caaactctcc cctcgtttcg catattcgcg ccgggtgttc
     8101 ggcgttggcg tccaggttgg tgcttcttgt ttacgttcaa cggtagtcac ccagttacgc
     8161 ggggtttctc gcccagcctg gccgatacca ataggaaaga cttccactgt attactgtct
     8221 ggcggatagt aataaagacg catttcagcg acgttaacca caatcccttt acgaacagtg
     8281 gcgggcaaaa tcagttgctg cggaatggtg agttgtgagc caggcttcgg caaaaaaaca
     8341 tcagcgcccg gattcgcttc cagcatatta cttaaccctt gcccgtattg tgcggcaaaa
     8401 gtctccagcg gctgagtatt gtgatcagga acagttacag taagcgactg ccccactaaa
     8461 cggctaccct ctggaggtaa tggataagtt accgccaggc tattttggct ggcaaaaagc
     8521 agagcaaatg agcaaagaat atttacacga cgcatcatgt ccctttccta agtcgcgaat
     8581 actatccgat aagtatagct tttatcagac ttttcgtttt tagctgttca aatcagaagt
     8641 cgtattcccc ggtagaacaa tattactggc agcaagttcg cccatgttgt tgtatatcgc
     8701 acaagcagct tcgatgatgg acatcgccag agctgcacca ctcccctcac ctaaacgcat
     8761 atccatattg agataaggct ccagccccaa atgcgagagc gctatacgcg cgcctttttc
     8821 tgccgacaag tgagaaggaa tgagatacgg tttgattgca ggagacatct ggcaggctgc
     8881 gagcgcagca gcataagaaa gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc
     8941 acctaacatc actccagcta ttccgaccaa atcaaatcca cccacttttg ccaggacatc
     9001 aacaccatcc tgaggatttg gttgattcaa cgtaatcgcc cgacgcacaa catcaatttt
     9061 attagccagt ttatctgtcg gcaggtttgc gccaatccca accacttctt caggatcccg
     9121 gccagtgatt gtgctgacta ttgccgctgc cggtgtcgtg tttgccatcc ccagttcacc
     9181 tacaccaaac agcgtgacac cgtttttcgc cagctcccgc gtataacata tgacgtccaa
     9241 aagcaacttt tcagcctgac ggcgactcat tgccggagct gaagcaatat tgccgctacc
     9301 tcgtgcgaca cgcatgttga taagcccggg gataggctca gcagtatcaa taccaacatc
     9361 aattacgtgg acgttagcgc ccgcttgtgc tgccagcaca cacacgccgg ttgttccacg
     9421 ggtcatattt tcagcctgta tggctgtcac ttcttttgga gaaatagcga ccccttcctc
     9481 ccagacgccg tgatcggcac acataaccag taccgctttt ttgcccacat gcggtatgcc
     9541 attcaccccc ggcattcctg ccagttgtat ggcaagcgcc tccagctttc ctaggctacc
     9601 aacaggtttg aataacccgt caacatgccg ttgtgcacgc gacatagcgg cagaattgat
     9661 ggctgggatc gtattcagta aatcggcaat tatttgcatg tcacgtttct ttatagcagt
     9721 gccagcagga agaccagttc accaagctct atggctgctc ccagcgtatc gcccgtttgc
     9781 ccgcccagcg tacgtttgag aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc
     9841 atagcagcca caccatgcat acccagcaat aatacggcag caaaaatagc ggctaagccg
     9901 agtgtgacgc aggtttgtcg cccatcaatt ttgccaatga acacattgcc aagcccttct
     9961 tcccgcgcat aacgatgacg atacatcaat aatgcggcag tcccgcggct gaccgcacat
    10021 gctgccgcca gcgatgcaag gatcggttcg ccacgcagag ccaactcgct taacaccaga
    10081 atctttgcca gtaccacaaa aatcaatgcc agaccgccgt gggtgcctaa acggctatca
    10141 cgcatgattt ccaacatgcg atcgcgacta cgtgcagaaa atacgccatc acaggtatca
    10201 gcgaggccgt ccagatggaa ttccccggtc atcagcgcca gcacaagtac gctaaacagt
    10261 gccgccagtg gtgcgccaca ccatgcctgc agcaccatga agaccagccc gctaatcgcg
    10321 ccaagcaata atccaatcaa aggaaaagta ataataccgc gagaataatg ctcgaaatcc
    10381 agtccctggg accagcgacg cggtacaggc agacgcgtaa taaatgagag catcgcccaa
    10441 aataatttgc tcatttgatt tttactccaa tacccgaaac caccagccat acttcatttg
    10501 ccgcagcggc caactgctga tttacccgcc cggcaatatc acgaaaatgt cgtgccagac
    10561 gactctccgg cacaatcccc attcccactt cgttagtcac taatacaacc tttgcggggc
    10621 aacgttggca ggcagcaatc aacgactgaa tctcagcatt aatcgcctgt tccatcgcct
    10681 gataatccca ttcatcaggg tctttatcgc cgccataatc aaacaacaga ttagtcacca
    10741 ttgtggtaac gcattccagc aacacagcct cattcgggtt aatgtctgca tgaattaatt
    10801 catcaagatg ttgccagcgc tccactgtgc gccagtgctc cgggcggctt tgccgatgat
    10861 gttctatccg tgcagccatc tcatcatcaa ggatttgcga ggtagcgata tacagaacct
    10921 gtgaa
//
LOCUS       DAEEGA010000112        10901 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEEGA010000112 DAEEGA010000000
VERSION     DAEEGA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10901
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.112"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(152..874)
                     /gene="baeR"
                     /locus_tag="KLH46_003465"
     CDS             complement(152..874)
                     /gene="baeR"
                     /locus_tag="KLH46_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBE0380024.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(871..2274)
                     /gene="baeS"
                     /locus_tag="KLH46_003466"
     CDS             complement(871..2274)
                     /gene="baeS"
                     /locus_tag="KLH46_003466"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBE0380025.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2271..3686)
                     /locus_tag="KLH46_003467"
     CDS             complement(2271..3686)
                     /locus_tag="KLH46_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0380026.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="KLH46_003468"
     CDS             complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="KLH46_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBE0380027.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="KLH46_003469"
                     /pseudo
     CDS             complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="KLH46_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(9875..>10901)
                     /gene="mdtA"
                     /locus_tag="KLH46_003470"
     CDS             complement(9875..>10901)
                     /gene="mdtA"
                     /locus_tag="KLH46_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBE0380028.1"
                     /translation="YLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEI
                     DPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETE
                     GTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPI
                     DLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKV
                     KARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKH
                     LVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREY
                     AKKGARS"
BASE COUNT         2510 a         3070 c         3003 g         2318 t
ORIGIN      
        1 ctaaaggtgc tgtttactga ttaaagtatg agcgattttc tgatgcctgc cagggcggag
       61 agataatgtc agtttgctga ccatgcgtgg aataatgttg ccggatcggg tctgaacgcc
      121 atctccggct aacaaagtaa tgtcgctaaa actaaacgat gcggcaggcg tcggcttccc
      181 agcggtaacc aacgccgtaa acggcgcgga taaatgactg ttcggcgtcg agagattcca
      241 gcttgcggcg cagattttta atgtggctgt caatggtgcg gtcggttact acgcggtagt
      301 cgtcataaag atgattgagc aattgctcgc gggagaacac ttttcctggt tcgtgagaga
      361 gcgttttaag cagacgaaat tccgcaggcg tcaggtcaag cattttaccg cgccatgaag
      421 cctgaaaacg accttcgtcg ataatcaacg ggctttcggc atcctgttgc tgaagttcgc
      481 gttgcggttt acaacggcgc aaaatggttt tgacacgcgc taccacttcc cgtgggctgt
      541 acggcttgca gatgtagtca tcggcgccaa tctccagccc cagcaggcgg tcgatctctt
      601 cgatttttgc cgtcaccatc acgatcggaa catcggaaaa acgacgaatt tcccggcata
      661 atgtcaggcc atcggtgcca gggagcatca gatccaacag gatcagatcc ggtggtgtct
      721 ggcgcacata cgccagtacc tgatcgccgt ggctgataag cgtcggcgca tagctcgcag
      781 cacgcagata atcaatgagc aactgcccca gcttcggttc atcttccacg atcaaaatac
      841 gcggtgtgtt ttcgtcgatt ggtaactcgg tcatacttct ctctgtaaat cccgttccag
      901 cggtaactct actgtaatgc ttaccccgcc aaaaggcgaa tgggcggcaa taatgcggcc
      961 attatgggct tcaacaatgt tcaggcaaat cgccagcccc agcccggaac cgccgctggc
     1021 gcggttgcgg gaaccttcgg tgcgataaaa acgttcaaac aatttttgta gctgatcgtc
     1081 actgacacct ggcgcactgt cggcaaaggt caggcgcacc gttttgtcgt gctgctcggc
     1141 agagattttc aggctgccgc cgctgtcagt gtagcgcagg ctgttttcca gtaagttatt
     1201 gaataactgc attaaacggt cgcgatcgcc aaatacggtg atactgtctg gcagggaaaa
     1261 ttgcagtttc aggccacggc tggcgaatcg ttcgcgaaat gcgccgcccg ccacttccag
     1321 cagtgggatc aaatctaccg gtgctttttg ataggcgaga gcgccttcat cagacatcga
     1381 caactgatga agatcgtcaa ccagtttggt cagtgtaccg acctccgcct gtaaagacgc
     1441 caccgtctcc ggcgtgaatt tgcgcacgcc atcctgaatg gcttccagtt caccgcgcag
     1501 caccgctaat ggcgtacgca gttcgtgaga aatatcggcc ataaaatcgc gccgcatttg
     1561 ctggtttttc tccagcgtgc tggcgagctg gttgaagtct tgcgccagtt tgcccagttc
     1621 atcttcactg gtgggcgtta cgcgggtagt gaaatcgccc gccgccagtt tgtgcgtgcc
     1681 atcgacaagt cgttttaccg gtgccagcaa accgcgcgcc agcagaaagg tggcgagcgc
     1741 cgcgagtaac gttgccaggg cgacgatcag ccagctggtt tgccgctgtt gtttatcgaa
     1801 attgatatca gtattgcggg tcagccgctc aacaggggag gcaatcaccg cgcccacttc
     1861 cgcaccgttg accagaatgg gtcgccgtgt accgtcaggt ggaatcggcg ctcgcggacc
     1921 aaccagtact ttgttgtttt gatcgaccac ccagaactgg gtacgccagc cgtgcggcgg
     1981 catacccggg ccgggtttat cttccgaatt atcgtgttca aatgaacgca ggatctgaaa
     2041 gacaaagcga tcgttgttgc gcaggaagcg ccagttacca tgctgcgcat actgctcgcc
     2101 aagcgcatca cttaacagct gtaatcgctg ttcattgccg tgtttgatgt aatcaataaa
     2161 gccacgctca aaactgatac gcaccgccca gtgcatactg atcagcaaga caatgcaggt
     2221 ggcgaaaatc gccagaaaca gtttgccggt aataccgggt cgccagaact tcattgcgcg
     2281 ctcctttttc gccgcgaaat agctacattt tgatgcgtat cgttcggcac tctggcaaag
     2341 atgaacgccg gaagggcgat gataaacgcc atgctaagcc aggtgtacat aaagacggtt
     2401 tgtgtggtgc cgctgtcgac gctgatatgc tgcgaaccaa aaagccccag caacagcccg
     2461 gcgatggtga cgccgatact catcgacaat tgcataatca tcgacagcag gctgtttccg
     2521 ctgctcgcca gattgtccgg gagatctttc agcgtcaggg tgttcatgga ggagaaacgg
     2581 gtcgagttga ccatcccttg taaaaacagg acgaacggca aaacgtagta ccagcccaac
     2641 agagcggtag tcataaacaa cagggtgacc agcgacagac ccagcgtggt cgctaccagt
     2701 acccgacgat aaccaaagcg attcaccacc tgtaccacaa ttcgcttcat tcccatgctg
     2761 ccaagcacca tcgggatcat catcagtccg gcatgaaacg gcgagaaacc gaggccaatt
     2821 tgcaggaaaa ccggtgtcat aaagggcaac atgccactgc caatacgtcc ggcaaagctc
     2881 cccgccaggc ccagcgaaaa ggtacgagtg cggaacagtt tcagactgaa cagggcgcgg
     2941 ttgttatttc tggcgtgcag cagataaagc accagtgcca ccacgccaac tgcgaccagg
     3001 ccagcaatcg ccagcggcga taaacctgta cctttactgc cgtccagcgc cagggttaat
     3061 accgccatgc caaccgccag caataaaaag ccggaaagat caaagcgccg cgtctgcatg
     3121 gtgtagttcg gcattaacat caatgtggcg atcgcaccga taatccccac cggaatgttg
     3181 atcaaaaaga tccagtgcca cgacgcgtat tctaccagca gaccgccgag cgccggaccg
     3241 agcagcggac cgacctgacc gggtaacgtg acaaaggtca tcgccgccat atattgctcg
     3301 cgcggtacga ttttcatcac cgtcaacctg ccgaccggca ccatcatcgc accgccaacg
     3361 ccctgtaacg cgcgtgccag caacagttcg ttcagcgtgc cggaaagcgc gcaaaacagt
     3421 gaaccgagag taaacagcac gatggcggta aagaaaatat tgcgcacgcc gactttgtcc
     3481 gccagccagc cgctggcggg cagcatcacc gccacggtca gcacataaga gacaatgacc
     3541 atgtgcatat gcaacggact ttccccgagg ctttgcgcca ttgagggaag ggcggtgttt
     3601 acgatggtgg tgtccagcga ctgcataaag aagccgaaag ccacaatcca taattgccag
     3661 cgggtgctgt cgggaagatc tgtcatttac tcggttaccg tttgtttagg tttacgcgaa
     3721 aaacgcagcc gcagacggtc gaaaaagaga tacaccaccg gcgtggtata cagcgtcagg
     3781 agctggctca tcaccagacc gccgacaatg gtgatctcca ggggttgccg cagctccgag
     3841 ccgtcaccgc ccgacaatac cagcggcagc gcgccaaaca gcgccgccag ggtggtcatc
     3901 ataatcgggc gaaaacgcag cagacaggcc tggaaaatag cttcctgcgg cgtcaggtta
     3961 ccgtgccgtt gggcttcaag cgcaaaatcg accatcataa tggcgttttt cttcacgatg
     4021 ccgattaata gcatgatccc tatcagggcg attaggctga acggggcatt gaacagctcc
     4081 agcgccaaca gcgctccaac gcccgccgag ggcagggtgg agagaatcgt cagcggatgt
     4141 acgtaactct cgtaaaggat acccagcacg atatacaccg tggcgatggc ggcgataatc
     4201 aggatcacct gcgagttcat cgtctcctgg aaaacctgcg ccgtgccggc aaaactgccg
     4261 cgcaccgtcg aaggcacacc aagctgggtc attgcgcgat cgatcgccgc actggcgtcc
     4321 gagagcgatt ttccggtcgg caggttgaac gagatggtcg aggccgccga taatccctga
     4381 tgattcaccg atagtggggc attcgccggt tgccatttag cgaaatacga cagcgggatc
     4441 gctttgcctt cgttattgat aacgaacatt ttttccagcg cactgatgtc ctgggtatag
     4501 cgcggatcca cttccatcac cactttatac tggttcatcg gctggtaaat ggtcgagatt
     4561 tgccgctgac cgaaggcgtt atttaacaga ctgttggcgg cctgtacgtc gattcccagc
     4621 cgtgccatgg tgtcgcggtc gtaaaccaga ttcatctccg cgccgttatc ctgctgatcg
     4681 gagttcacgt ccgccagttc cggcaacgtc gccagttttt tgcggatttt cggctcccat
     4741 tcgcgcagtg ccgccaggtc gtcggataac aacgtgtact ggtagctggc gttcgactga
     4801 cgcccaccaa cgcgaatatc ttgtaccgcc atcaggaaca gattcgcccc cggttctttc
     4861 gccagcttca cgcgcagacg gtcgataatt tgctgcgccg tttcgctgcg ttcgtcgcgt
     4921 ggcttgaggg tgataaacat catcccactg ttaactcgtg aaccgccggt aaagccggtg
     4981 acattatcca ctgccggatc gtcacggata attttcatga aatcctgcaa tttgccgcgc
     5041 atcgcctgaa acgaaatact ctgatccgcc tgtatcccac ccatcagtac gccggtgtcc
     5101 tgctccggga agaaggtttt cgggatcgag atattcagcg caatggtgcc aagcagcacc
     5161 acgcccacca gacgggtatg attgagcacc catttcagcg acttgccgta gccttgttgc
     5221 agggctacca acatgcggcc aaaaccacgc agtcgctttt gctcacgcgg cttgctggct
     5281 tttagcatcc agccgcacat cattggcgtt agcgtcaggg aaaccagcaa cgaaatacca
     5341 atggcgacag aaagcgtcac ggcaaactcg cgtaacagtc ggcccggcaa tccgcccatc
     5401 aacagcagcg gcaggaacac cgccaccagt gacagactca tcgacagcac cgtaaagccg
     5461 acttcgcgag taccttgcag cgcggcctgt agtggtttca tccccgcttc cagatgacgg
     5521 gcaatgtttt ccagcaccac aatggcgtca tccaccacga aaccagtagc aatggtgagc
     5581 gccatcagcg aaagattatt gagactgaat ccgcacaggt acatcgccgc aaacgtacca
     5641 atcagcgaaa ccggcaccgc aacggcggga ataatagtgg cgcgacccga gcgcaggaat
     5701 aaaaagacca ccagaatcac cagcgccacc gagataatca gcgtttgctc gacttcttcc
     5761 agcgaggcgc gaatggtggg ggagcgatcc tgggcaattt gcagatcaat cgccgccgga
     5821 atggtctcct gcaactctgg taattttgcc cggatgctgt ctaccgtctg gataatattg
     5881 gcttccggca gtttgcggat catcagtaaa atagccggtt tggcgttggt catcccggcg
     5941 ttgcgcacat cctgcactga gtcagtcacc gtcgccacat cgcccagacg aaccgcgccg
     6001 ccgttgttgt agtgaataat cagcggctga tattcagcgg cggttttcag ttcatcgttg
     6061 gtctggatct gccagcggtg agtgccatct tccagcgccc cctgcggttt acgcacgttg
     6121 gcattgctga tggcagtgcg tacgtcgtcc agcgacacgc cctgattaaa cagcgcctgc
     6181 ggggtcagcc cgacgcgcac ggcgggcagc gagctgccac cgacatcgac atcaccgaca
     6241 ccgtcgattt gcgaaatcgt cggagccagc tgcgtcgagg cgaaatcgta cagttcaccc
     6301 tgcgaataag tatcggatgt cagcgtgagg atcataattg gcgcatccga cgggttcgct
     6361 ttgcgatagg tcgggcggct gggcatcccg ctgggcagca aactttgtgc agcgttgatc
     6421 gccgcctgca catcacgcgc tgcgccgttg atatcccggt caaaatcaaa ctgcaaaata
     6481 atgcgcgtgc tgccgagcga actgctggag gtcatttcac taaccccggc aatgcgccca
     6541 agtgagcgct ccagcggcgt ggcaacggaa gacgccattg tctccggcga cgcaccgggc
     6601 agcgaggcgc tgaccataat caccggaaaa tcgacctgcg gcagcggggc gaccggcagc
     6661 atacggaaac ccagtatgcc gcacagggta atggcgaccg acagtaaaat cgtcgccacc
     6721 gggcggtaaa tgaagagggc aaaaaacttc acttacgcct cctcttcatg gcgagcaaag
     6781 cgacttttgg tccacaatgc caggcggtcg aacagcagat aaatcaccgg cgtggtgaac
     6841 agcgtcagca cctggctgac aatcagaccg ccgaccatgc cgatacctaa cggacgacgc
     6901 agttccgcgc cgaccccggt actcaacatc agcggcagcg cgccaagcag agccgccagg
     6961 gtggtcatca ggatcggacg aaaacgcaac agacaagcct ggtagattgc atcgcgcggc
     7021 gacatgcctt gctcgcgctc agcagccagc gcgaagtcga tcatcatgat ggcgttcttc
     7081 ttcacaatac cgatcagcaa aataatgccg ataatcgcaa tcacatccag ttcgctaccg
     7141 gcaatcatta atgccagcag cgcgccgacc cctgcggtgg gtagcgtcga gagaatggtg
     7201 atcgggtgaa taaagctctc gtacagaatg ccgagcacga tatacatcgc caccaccgcc
     7261 gcgacaatca gccagacagt gctgcccagc gccgactgga aggcgagggt gctgccctag
     7321 aattgcgtgg tgatatccac cggcagattc agcgtctttt cggtgtccat aatcgcctgc
     7381 accgcatcgc ccagcgaata gttatccggc acgttaaagg agatggttgt taccgggaac
     7441 tgatccagat ggttgatgga aagcggcgca aaacgctgct caattttggc aattgagctt
     7501 aacggcacca cgccaccgtc gctgctggtc aggcgaatgg tatccagcgc cgcgaggcct
     7561 ggggtatttt cggtattgtg ctccagcacc acgcgatact ggttagcctg agtataaata
     7621 gtggaaatca gtcgctgacc aaacgcgttg tacagggcgt tatcgacgtc cgccatgctg
     7681 atccccagac ggctggcgct gtcgcgatca acattgacat acgccaccag ccctttgtcc
     7741 tgccagtcgc tggagacatc agaaagctgt ggcagttgct ggagtttttc catcaactgt
     7801 ggcacccagg tactgagcgc atccagtgac gtggcctgca aggtaaactg gtactgggtg
     7861 cggctgacct gagtatcgat ggtcaggtcc tgcgttggtt gcaggaagag atcgacgccc
     7921 ggcactttat ctaccgccgt ttgcagacgg gcgatgactt tttgcacccg atcatcacgt
     7981 tcatccaacg gtttgaggtt gatttgtaaa cgtgcactat tcagcgacgg gttagtgcca
     8041 tcaacgccaa caaatgaggt caggctttgc actgccggat cctgcaaaat cacgtccgcg
     8101 acctggcgtt gtcgctgggc catattggca aaggagctgg attgcggtgc ctgcaaagtg
     8161 ccctgaataa tgccgttgtc ctgtaccggg aagaaacctt tcggaatgaa cacccacagc
     8221 agcacgctaa gcagcagcgt gctgagtgcc acgcttaagg tcagccacgg atgattcagc
     8281 actttcgcca gtccacgacc ataggcggcg attatcctgt cgaacatttt ttccgaggca
     8341 cgggagaagc ggttctgttt acgcaacgac tcctggctga gcatccgcgc gcacatcatc
     8401 ggtgtcaggg tcagcgacac caccgctgag atcaaaatcg ctaccgccag ggtaatagca
     8461 aattcgcgga acagtcgccc gacgatatcg cccataaaca gcagtgggat caacaccgca
     8521 atcagtgaga aggtcagcga gataatggta aagccgattt cacctgcgcc cttgagcgcc
     8581 gccgccaacg gtttttcgcc tttttcgata tagcgggaaa tgttttcgat caccacgatg
     8641 gcgtcatcga ccacgaatcc ggtggcgata gttaacgcca tcagtgtcag gttattgatt
     8701 gaaaaatcga gaaacaccat aaccgcgaaa gtgccgatta acgacagcgg tacagcaaca
     8761 ccgggaatga tggtcgccgg aatattgcgc aaaaacaggt agataatcat gactaccagc
     8821 gcgatagcca tcatcaattc aaactgagta tcatcgacgg atgcgcggat attggtggtg
     8881 cgatcggaaa gcactgtcac cttcaccgat ttcggcagac tctcagtgag ctgtggcagc
     8941 atctgccgaa tgctgtcggc ggtggagata atgttagcac cgggctggcg ctgaacattc
     9001 atcacaatgg cctgttcttt gttcgcccac gcgccgagcc agctgttttc tgcaccttgc
     9061 tctacagttg cgacatcgcc cagacgaatt ggcgcgccgt tctggtaggc gatgattagc
     9121 tggcgatact cttcggcgga ttgcatctgg tcgttcgcgg aaagcgtgac cgcacgggaa
     9181 gggccgtcga ggctaccttt tgccgagtta acgttagcgc cggtaatggc ggtgcgcacg
     9241 gtttcgctgg tcaggccgag ggcggcaatc gcctgagcgt taagtttgac gcgaacagcc
     9301 ggacgctgac cgccggaaag cgtcaccagg ccgacgccgg aaatctgcga gattttctgc
     9361 gcgacgcggg tttccaccat atcttccact tgcgtcatcg gcatggcggt tgaggtgacg
     9421 gcgagcgtca tgatcggcgg atctgccggg ttcactttgc tgtaaaccgg cgggttaggc
     9481 agatcgctcg gcaacaagtt ggtcgcagcg ttaatcgcgg cctgcacttc ctgctcggcg
     9541 acattgagcg gtaatgttag ctggaactgc aaagtgataa ctgacgcacc gccggaactt
     9601 tgcgacgaca tctgtttcag gccagacatc tgcccgaact ggcgttcgag cggcgcggta
     9661 acggcagagg tcatgacatc cgggctggca cctgggtaga gcgtgaccac ctgaatggtc
     9721 ggatagtcca cttccggcag cgccgaaacg ggcagggcgc gataaccgat aatcccggcg
     9781 agtaagatcg ccaccatcag cagcgtggtg gcgacaggac gcataataaa caggcgcgac
     9841 gggccgcctg tgctgctcgg gggtaacacc tgcatcagga gcgtgctcct tttttcgcgt
     9901 attcgcggct ggtggctttc tcttccggag tggtggcgct ctgggcttcc accacttcca
     9961 ctttcgcccc ttcggtcagg cgatcaatgc cgtctgtcac cacgcgatcg cccgcagaaa
    10021 tacctgcacg gatcaccact ttctgactgt cctgaatgcc cggcgtcacc agatgtttgc
    10081 tgaccttgtt ttcgctattc agcacccaga caaaatggcc ttcattgccc atttgcaggg
    10141 cggctgttgg gatcactacg gcgttttgtt cggtgtcgac taacatgcgc gcgttaacaa
    10201 actgattggg aaacagcgca tcatcctgat tattaaagcg tgctttcact ttaatcgtac
    10261 cggttgtggc atcgatttgg ttatcgaggc ttaacagcgt gccttcactt aatttcttcg
    10321 aattggtgcg atcccaggct tctaccatca gcggttttcc ggctttttgc gcctgcacta
    10381 cagtagcgat atcgctttcc ggcagggtaa agactaaatc gataggatgc gtctgggtga
    10441 tcaccacaat tccggtggta tcaccactgg agatttggtt accaacatca acttgcttga
    10501 gaccaacgcg accatcgact ggtgcggtga tgcggctcca gtcgagttgc aactgcgcgc
    10561 tggcgacgct tgcttcatca gccttaatgg tgccttcggt ttcactgacc agcgcctgtt
    10621 gggcatccag ctcctgacgg gagacgagat tggtttttgc cagttgctga taacgcgcca
    10681 gatcacggcg ggcgttagca agcgtggctt tatcttttgc cagttggccc tgggtttgtg
    10741 ctaatgcaac tttgaactgg ctggggtcaa tttctgccag taaatcgcct gctttgacct
    10801 gctggccttc ctggaaatgt aacgccatca gttgaccgtc cacgcggctg cgcaccgtaa
    10861 cagtattagc ggcggtaatg gtgccaagcc cggtgaggta a
//
LOCUS       DAEEGA010000113        10746 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEEGA010000113 DAEEGA010000000
VERSION     DAEEGA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10746
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.113"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(132..1481)
                     /gene="lysC"
                     /locus_tag="KLH46_003471"
     CDS             complement(132..1481)
                     /gene="lysC"
                     /locus_tag="KLH46_003471"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBE0380029.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLELGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2006..3655
                     /gene="pgi"
                     /locus_tag="KLH46_003472"
     CDS             2006..3655
                     /gene="pgi"
                     /locus_tag="KLH46_003472"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBE0380030.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4117..4359
                     /locus_tag="KLH46_003473"
     CDS             4117..4359
                     /locus_tag="KLH46_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380031.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            4473..5111
                     /locus_tag="KLH46_003474"
     CDS             4473..5111
                     /locus_tag="KLH46_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBE0380032.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5108..5845
                     /locus_tag="KLH46_003475"
     CDS             5108..5845
                     /locus_tag="KLH46_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBE0380033.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5845..7941
                     /locus_tag="KLH46_003476"
     CDS             5845..7941
                     /locus_tag="KLH46_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBE0380034.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7988..8266)
                     /locus_tag="KLH46_003477"
     CDS             complement(7988..8266)
                     /locus_tag="KLH46_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380035.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8267..8410
                     /locus_tag="KLH46_003478"
     CDS             8267..8410
                     /locus_tag="KLH46_003478"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380036.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8536..8946
                     /gene="psiE"
                     /locus_tag="KLH46_003479"
     CDS             8536..8946
                     /gene="psiE"
                     /locus_tag="KLH46_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBE0380037.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8990..10465)
                     /gene="xylE"
                     /locus_tag="KLH46_003480"
     CDS             complement(8990..10465)
                     /gene="xylE"
                     /locus_tag="KLH46_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HBE0380038.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         2727 a         2740 c         2777 g         2502 t
ORIGIN      
        1 tttttctgct cattattagc tccttctgat gccattctat ttcaggaagg agtgtccgtt
       61 aaactcaggc tacctcatag tgacaagaaa atcaatacgg cccgaaatat agcttccagg
      121 ccatacagta tttactcaaa caaattacta tgcagttttt gcaccacctg ctcggcatct
      181 tcgccgggca ccaggaagca caggttatgg ctggatgcgc cgtaacaaat catgcgaatg
      241 ttgaacggtt ccagtacgcc gaatacctct ttgccaacgc cgcaggcttt tgacaggtca
      301 ttgccaatca acgcgaccag cgccagacct tcttccacct ccacccggca cagtgcggaa
      361 agctccatca gcagagattg cgtcagcaac gtatcgccag tggaggttga accggtggta
      421 tcaagggtta atgccacgct cacttctgac gtggtgatta agtctaccga aatattatgc
      481 cgcgcgagga tgccgaaaac ttccgcgagg aagccgcgag aatgcagcat attcaggctg
      541 tgcaaagtga gcagagtctg attgcgacga agcgccagcg cgcggaacag cggcggattt
      601 tcagttttat tgcacaccag cgtaccacct gcgcgtgggt ctttgctgga gccgacaaag
      661 accgggatat cgctgcgtac tgcgggtagc aacgttgccg gatgcagtac ttttgcacca
      721 aaagttgcca tctctgccgc ttcggcaaac gcgatttcat caatgcgttt tgctgcggaa
      781 actacgcgtg gatcggtggt gtagatgccc gggacgtcgg tccagatatc aacacgagat
      841 gcgtgtaaag cctccgccag caaggctgcc gtataatcgc tgcctccacg gccaagcgtc
      901 gttgtacgac ctttattttc gctaccgata aatccctggg tgatcactaa gccttcattg
      961 aggcgtggga gcagctgcag cgcggccagt tccgccagcg cggctacatc tggctctgca
     1021 cgaccaaatc ggtcgttggt acgcatcact ttacgtacat caaaccactg tgcctgaaca
     1081 tcgcgttcgc gcaggatttc gacaaacagc agggtcgaca tcagctcgcc gtggctgacc
     1141 agctcatctg tcagcgccgg agacgttgcc agcgccgccg cttctgccag aacagtaatg
     1201 ttctccagca gacgttcaat ctcttcacgg ataacgttcg ggtaacgcag acgttccaga
     1261 atggcaaact ggatgttgcg aatagcgtcg agtttttcga atcgctcgcc aagttccagc
     1321 ccttccgcta aagcgaccag cagattagtg ataccagcag aagccgagag gactactaaa
     1381 cgcacgttgg catcagaaag cacaatatca gcgctgcggt tcatggcgtc aaaatcagct
     1441 acgctggtac cgccaaattt ggagacaaca atttcagaca taactacctc gtgtcagggg
     1501 atccattttc agccttggca caagggaaga gcggaagacg ggtgggcgca gagcgatact
     1561 tcgctactat tttcacccag aagtgctcca ccacttgcga aacgcccgac tgcgaacgct
     1621 tctggtgaca acccagggga ttcagcccct gtagccgatg atgaacgtgg ccagccgttc
     1681 aatcacctcg gcgatgcacc ccctcaggtg ttatcacagg actggctcct ccaacaccgt
     1741 tacttgggca acgcgcctct tctggcctgc gctagcgcag gtagtacatt tataaataaa
     1801 gggtgagcgg ggcggttgtc aacgacgggg tcatgcggat ttttcatcca ctcctggcgg
     1861 tcagtagttc agctaataaa tgcttcactg cgctaagggt ttacactcaa cattacacta
     1921 acggcactaa aaccatcaca tttttctgtg actggcgcta caatcttcca aagtcacaat
     1981 tctcaaaatc agaagagtat tgctaatgaa aaacatcaat ccaacgcaga ccgctgcctg
     2041 gcaggcacta cagaaacact tcgatgaaat gaaagacgtt acgatcgccg atctttttgc
     2101 taaagacggc gatcgttttt ctaagttctc cgcaaccttc gacgatcaga tgctggtgga
     2161 ttactccaaa aaccgcatca ctgaagagac gctggcgaaa ttacaggatc tggcgaaaga
     2221 gtgcgatctg gcgggcgcga ttaagtcgat gttctctggc gagaagatca accgcactga
     2281 aaaccgcgcc gtgctgcacg tagcgctgcg taatcgtagc aataccccga ttctggttga
     2341 tggcaaagat gttatgccag aagtcaacgc ggtgctggag aagatgaaaa ccttctcgga
     2401 agcgattatc tccggtgagt ggaagggtta taccggcaaa gcaatcactg acgtagtgaa
     2461 catcgggatc ggcggttctg acctcggccc gtacatggtg accgaagcac tgcgtccgta
     2521 caaaaaccac ctgaacatgc actttgtttc taacgtcgat gggactcaca tctcggaagt
     2581 gctgaaaaaa gtaaacccgg aaaccacgct gttcctggta gcatctaaaa ccttcaccac
     2641 tcaggaaacc atgaccaacg cccatagcgc gcgtgactgg ttcctgaaag cggcaggtga
     2701 tgaaaaacac gttgcgaaac attttgcggc gctctcaacc aatgccaaag ccgttggcga
     2761 gtttggtatt gatactgcca acatgttcga gttctgggac tgggtcggcg gccgttactc
     2821 tttgtggtca gcgattggcc tgtcgattgt tctctccatc ggctttgata acttcgttga
     2881 actgctttct ggcgcacacg caatggacaa gcatttctcc accacgcctg ccgagaaaaa
     2941 cctgcctgtc ctgctggcgc tgattggcat ctggtacaac aatttctttg gtgcggaaac
     3001 tgaagcgatt ctgccgtatg accagtatat gcaccgtttc gcggcgtact tccagcaggg
     3061 caatatggag tccaacggta agtatgttga ccgtaacggt aacgttgtgg attaccagac
     3121 tggcccgatt atctggggtg aaccaggcac taacggtcag cacgcgttct accagctgat
     3181 ccaccaggga accaaaatgg taccgtgcga tttcatcgcc ccggctatca cccataaccc
     3241 gctctctgat caccaccaga aactgctgtc taacttcttc gcccagaccg aagcgctggc
     3301 gtttggtaaa tcccgcgaag tggttgagca ggaatatcgc gatcagggta aagatccggc
     3361 aacgcttgac tacgtggtgc cgttcaaagt gttcgaaggt aaccgcccga ccaactccat
     3421 cctgctgcgc gaaatcactc cgttcagcct gggtgcgttg attgcgctgt atgagcacaa
     3481 aatctttact cagggcgtga tcctgaacat cttcaccttc gaccagtggg gcgtggagct
     3541 gggtaaacaa ctggcaaacc gtattctgcc agagctgaaa gatgataaag aaatcagcag
     3601 ccacgatagc tcgaccaatg gtctgattaa ccgctataaa gcgtggcgcg gttaatcatc
     3661 atgcgtgcct gatgccgata tgtaggccgg ataaggcgtt tacgccgcat ccggcaaccg
     3721 gagcctgatg ccaatatcga cattaatgca tagtatgagg agttaaataa ccttaaaaac
     3781 aatgccgaca ataagtcggc attgttttat cagataaatc cccttgtctg tgatttaacg
     3841 gaaatcatac cgtgaggtta atcctaaaat agatttttaa tcgttgttta tttcggagaa
     3901 tacgcagatt aattactttt gtttttattt taagcttatg atttttattg ttatttaaat
     3961 ataagttgaa acttatattt gatattcatt ccaattatcc taaaactcca tcgctaattc
     4021 cccgcgccgt aattcgcatg ctttagttgt gtatactcga tcccgcccga aatgtttttg
     4081 ggtaaatctc cattcattca atgaagggaa attgttatga aaaaagttct gtatggcatt
     4141 tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg cacctgtaca ggtgggcgaa
     4201 gcggcagagt cggcagcaac gtcggtttcg gcggggagtt cctccgcgac cagcgtcagc
     4261 accgtaagct cggcggtggg tgtcgcgctc gcggcaaccg gtggcggtga tggttctaat
     4321 accgggacca tcacaaccac gaccaccagt acccagtaat aaagtatgta tccccaaaat
     4381 aatttgagtc attgcatctg tggcttgaag tatgaaggga tttaccataa ccacactccg
     4441 gtgtggttat tctgcccctc tggagaagag tcgtgaagcg acctgcactc attcttatct
     4501 gcctgctatt acaggcctgt tcagccacga ctaaagagct gggcaattca ctgtgggaca
     4561 gtctgttcgg cacgccaggc gtacagctga cggacgatga tattcaaaat atgccctacg
     4621 ccagccagta catgcagctt aatggcgggc cgcaattgtt tgtggtgctg gccttcgctg
     4681 aagacggaca acaaaaatgg gtcactcagg atcaagccac tcttgtcaca caacatggtc
     4741 gtctggtgaa gactttgctt ggcggcgaca acctgattga agtgaataac cttgccgccg
     4801 acccgctgat taaacccgca caaattgttg atggtgcaac atggacgcgc acgatgggct
     4861 ggaccgagta ccagcaggta cgctacgcca ccgcacgctc agtcttcaaa tgggatggca
     4921 ccgataccgt caaggtcggc agcgatgaaa ccctggttcg cgtgctggac gaagaagtct
     4981 ccaccgacca ggcgcgctgg cataaccgct actggatcga cagcgaaggg caaattcgcc
     5041 agtcggaaca gtatctcggc gcggattatt tcccggtgaa aaccactctc atcaaggcgg
     5101 caaaacaatg attaaacaaa ctattgtcgc gttgcttttg agtgtgggag cgtcatcggt
     5161 cttcgcggca ggaaccgtca aggtgttcag caatggcagc agtgaggcca aaacgctaac
     5221 gggcgcagag catttaatcg atctggtagg gcaacctcga ctggcaaaca gctggtggcc
     5281 tggtgcggtg attagcgaag agctggcaac ggcggcagca ttgcgtcagc agcaggcgtt
     5341 gctgacacgg ctggcagaac agggcgcaga ttccagcacc gacgatgccg ccgccattaa
     5401 cgccttacgc cagcaaatcc aggcattgaa agtgacaggc aggcaaaaaa tcaatcttga
     5461 tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg ttgcagggca actacacgct
     5521 gtgggtcgga ccaccgccgt ccacggtcac gttgttcggg cttatcagcc atcctggcaa
     5581 tcagccattc actccaggtc gcgacgtggc gagctatctc tctgaccaga gcctgctcag
     5641 cggtgcggat cgcagctacg cgtgggtcgt ttacccggac ggacgcacgc aaaaagcgcc
     5701 ggtggcttac tggaacaagc gtcacgtaga gccgatgccc ggcagcatta tttatgttgg
     5761 cctcgcagat tccgtctgga gtgagacgcc tgatgccctt aacgccgaca ttcttcagac
     5821 tctgacgcag cggatacccc aataatgaaa aaaagacatc tgcttagctt actggcgctg
     5881 ggcattagca cagcttgcta cggcgaaaca tatcctgcgc ccattggtcc gtcgcagtcg
     5941 gatttcggtg gcgtaggatt attacaaact cccaccgcgc gtatggcacg ggaaggggag
     6001 ttgagtctga actatcgcga taacgatcag taccgttatt actcagcttc agtgcaactc
     6061 ttcccgtggc tggaaacaac gctgcgctac accgacgtgc gcacccggca gtacagcagc
     6121 gtcgaagcgt tctctggcga tcaaacgtat aaagataaag ccttcgatct caaactgcgt
     6181 ttgtgggaag agagttactg gctgccgcaa gtggcggttg gtgcgcggga tatcggcggt
     6241 acggggctgt ttgatgcgga atatcttgtt gccagcaaag cctgggggcc gttcgatttt
     6301 acgctcggcc tgggctgggg atatctgggc accagcggta atgtaaaaaa tccgctctgt
     6361 tcagccagtg ataaatattg ctatcgcgat aacagctaca aacaggcggg atctatcgac
     6421 ggtagccaga tgttccacgg tcctgcctca ctgtttggcg gcgtggaata ccagacgccc
     6481 tggcaaccgc tgcgtctgaa actggagtat gaaggtaata attatcagca ggattttgcc
     6541 gggaagctgg agcaaaaaag taagtttaac gtcggggcga tttatcgcgt taccgattgg
     6601 gccgacgtta accttagcta tgaacgtggc aacaccttta tgtttggcgt cacgctgcgc
     6661 accaacttta acgatctgcg cccgtcctac atcgataacg cccgcccgca atatcaaccg
     6721 cagccgcagg atgccatttt gcagcattcg gtagtggcga atcagttaac gttgctgaaa
     6781 tacaacgccg gacttgccga tccgcagatc caggcgaaag gcgatacgct gtatgttacc
     6841 ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca tccgtgctaa tcggatcgtg
     6901 atgaacgatc tgccggatgg gatcaaaacg atccgcatta cggaaaatcg ccttaacatg
     6961 ccgcaagtga cgacggaaac cgatgtcgcc agcctgaaaa atcatctcgc cggagagccg
     7021 ttgggccacg aaacgacgct ggcgcacaaa cgcgtcgagc cagtggttcc gaagtccacc
     7081 gagcagggct ggtatatcga caaatcacgc tttgatttcc atatcgatcc ggtgctgaac
     7141 cagtcggtcg gtggcccgga aaacttttac atgtatcagc tgggcgtgat gggaacggca
     7201 gatttgtggc tgacggacca tctactgacc accggcagcc tgtttgcaaa tcttgccaac
     7261 aactacgaca agtttaacta caccaatcca ccgcaggact cgcacttacc gcgcgttcgt
     7321 acccatgtgc gcgagtatgt gcagaacgat gtctatgtga ataacctgca agccaactac
     7381 ttccagcatc tgggcaacgg cttctacggt caggtctatg gaggttatct cgaaaccatg
     7441 tttggcggtg cgggggccga agtgttgtat cgtccgctgg acagtaactg ggcgtttggt
     7501 ctggatgcca actacgttaa acagcgcgac tggcgtagtg caaaagatat gatgaaattc
     7561 accgactaca gcgtgaaaac cggacatctg accgcctact ggacgccatc tttcgcccag
     7621 gacgtgttgg tgaaagccag cgttgggcag tatctggcag gggataaagg cggcacgctg
     7681 gagatcgcta aacgctttga cagcggcgtg gtggtgggtg gctatgccac gatcaccaac
     7741 gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag gcgtgtatgt ctcggtaccg
     7801 ttggatctct tctcgtctgg cccgacacgc agccgtgcgg cgattggctg gacgccgctg
     7861 acgcgtgacg gtggtcagca acttgggcgt aagttccagt tgtatgacat gaccagcgac
     7921 cgtagcgtaa atttccgcta agtcatggga aaggtgccag ttttcgcact cactggcacc
     7981 ctcattctta atgacctgtt gagccatcag gaacgcagtt cacccccggt ttacgatctg
     8041 gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga tatcggttgc aggtctggtt
     8101 gcctgagcga ctgggccatc attgggctgg agacattgct ttgcggtagg tcggctttat
     8161 cagcagtacc gagcggacca gcataagcag gaagaacaga gactgataac atcaaagcag
     8221 caaaataagg cttcattttt accaccttta tcaggttacg tttcatttgt tccagaggaa
     8281 cattgtcgat ttttcgcgca ttgctggtgg ctgggaatca cctgaatggg tgatttttga
     8341 attaccggct ttggtgcggt ttgtcttgcc ggatgcgccg ccaggcgcgg cttatccggc
     8401 ctacgggtag gtatatccgg ctttggtgga ggcgcgctcc aaatccaggt tgaacaaaac
     8461 atacacaaaa aatatagatc tccgtcacat ttttgcgtta tacaggaagc tcgccactgt
     8521 gaaggaggta ctgctatgac gtcactctct cgtccgcgcg tggagtttat ctccaccatt
     8581 ttgcagaccg tactcaatct tggcctgctg tgcctcggcc tgattttggt tgtcttcctc
     8641 ggcaaagaaa cggtgcatct ggctgatgtg ctgttcgcgc cagaacaaac cagcaaatat
     8701 gagctggtag aaggactggt ggtttacttt ctctatttcg aatttatcgc gctgattgtg
     8761 aagtactttc agtccggttt tcacttcccg ctacgttact ttgtctatat tgggatcacc
     8821 gccattgtgc gcttgatcat cgtcgatcat aaatcgccac tggatgtgtt gatctactcg
     8881 gctgcgatcc tgttactggt gatcaccctg tggctgtgta attcgaaacg gctgaagcgg
     8941 gagtaaaaag acagcacgcc gaaatggcgc ggcgtgctgg acaggaagat tacagcgtag
     9001 cagtttgttg tgttttcttc gtttccggtt cccagagcgc ttccagctcc tcaagggttt
     9061 tacctttggt ttccgggaca aatttccaca taaacagtgc tgccagaacg cccatacaac
     9121 cgtaaatcca gtaggagaaa ccgttgtgga aatgggccac cagccaggag tttttgtcca
     9181 tcatcgggaa ggtccaggag acgaagtagt tcgccagcca ctgggccgcc accgcgattg
     9241 ccagcgcttt accacgaata gcattcggga agatttccga cagcagtacc cagcataccg
     9301 gaccccagga catggcaaag gcggcaacat agaacagcat cgacagtagc gccacaatac
     9361 ccgatgcctg agtgtaaaac gcggtaccga ggctaaacat accgattgcc attccgagtg
     9421 cgccgataat ttgcagtggc ttacgaccaa atttatccac agtcataatt gccagtacgg
     9481 taaaggtgag gttgataact ccgacaataa tggtctgcaa cagcgcgata tccgtgctgg
     9541 cccccagcgt tttgaacact tccggcgcgt agtacagcac cacattgatg ccgacaaatt
     9601 gctggaagat ggagagcata acgccgatta caatcacgcc cacgccaaac atcagcagac
     9661 gaccaccggt tttgcggcca tgatccaggg agtgtttaat ttcctgtact gcctgagttg
     9721 caagcgtgtt gcccataatt ttgcgcagga tactttccgt ctgttcttgc ttgccgcgtg
     9781 acatcagcca gcgaggactt tctggcacgg tatacagcag cattaagaac agcagtgcag
     9841 ggatacattc cgaggcaaac atataacgcc tgccgtcagt attcagccag ctggcatcac
     9901 cggaacgggc aataaaatag tttacgcagt aaactaaaag ttgcccgaaa ataatcgcaa
     9961 actggttaaa tgaaaccagt tttccgcgaa tatgagctgg agccagttcc gcaatataca
    10021 ttggtgaaag cattgaggct aaaccaacgc caataccgcc aataatacga taaataacaa
    10081 attccgggac ataacctgcc agataaatag gtactgtgtt gtccgggttt atagaggtaa
    10141 aaccaagttc tggccaggca gaacctacac cagaaataaa aaacaggaca gcagaaatct
    10201 taagtgaatc acgacggccg aagcggttac tgcaataacc accgagggca ccgccgatga
    10261 tgcaaccaat cagagcgctg gctacgcaaa atcctaacag ggagttggca gcggattcac
    10321 ttaagttttg tggagcaaca aagacggtat tgagtgactc aacagtaccg gaaataacgg
    10381 cggtgtcgta gccaaataat aaaccaccta atgtagcgac taaggtaatc gaaaatatat
    10441 aactggaatt atactgggta ttcattcaga cctgccttag accattctga tcttttatca
    10501 gtgcattgat gttggaattg tcaggcagtt aaggcaatta tcatttttta caatgcgttt
    10561 acgtaatgtc ttttctgtga tcttaattgt gataattatc aaaaattgat gaaaacaata
    10621 ctattgccgt gactcagagc acgaaagaga attatcgtaa gtgggaaaac aaataacgta
    10681 aaaataataa gttctatgat gggaatatta accggcgaac gattcagatt gcagacgaaa
    10741 gaaaaa
//
LOCUS       DAEEGA010000114        10686 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEEGA010000114 DAEEGA010000000
VERSION     DAEEGA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.114"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..213)
                     /locus_tag="KLH46_003481"
     CDS             complement(<1..213)
                     /locus_tag="KLH46_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBE0380039.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSS"
     gene            704..835
                     /locus_tag="KLH46_003482"
     CDS             704..835
                     /locus_tag="KLH46_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HBE0380040.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            1095..2090
                     /gene="pgl"
                     /locus_tag="KLH46_003483"
     CDS             1095..2090
                     /gene="pgl"
                     /locus_tag="KLH46_003483"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBE0380041.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2131..3084)
                     /locus_tag="KLH46_003484"
                     /pseudo
     CDS             complement(2131..3084)
                     /locus_tag="KLH46_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3268..4320
                     /locus_tag="KLH46_003485"
     CDS             3268..4320
                     /locus_tag="KLH46_003485"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBE0380042.1"
                     /translation="MKKIPCVMMRGGTSRAAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            4396..5829
                     /locus_tag="KLH46_003486"
     CDS             4396..5829
                     /locus_tag="KLH46_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBE0380043.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AMALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            6012..8192
                     /locus_tag="KLH46_003487"
     CDS             6012..8192
                     /locus_tag="KLH46_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBE0380044.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMASGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(8333..9616)
                     /locus_tag="KLH46_003488"
     CDS             complement(8333..9616)
                     /locus_tag="KLH46_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBE0380045.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9751..10632)
                     /locus_tag="KLH46_003489"
     CDS             complement(9751..10632)
                     /locus_tag="KLH46_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0380046.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2623 a         2462 c         2662 g         2939 t
ORIGIN      
        1 cgaggaaagc gccaaggctc gcaggtccag tttatgcccc ttagaggtaa gacatgcacg
       61 aagtcttctt accgcctcag tacgctgttc ttccggtaac ccctgtttct cccaatcatc
      121 ccattcggac aaataatggt tgggggatgt aattgtgctg atatgtggaa ataaacagga
      181 tgaaatgttt ctgagcatat taatttctcg catatggata tggaattaga ttgtgaataa
      241 attgtattta atgtatctct cctaatgagg agggtgtttc agaatattcg ccataaggga
      301 atgagtgtta tatcatgctt tctaccctgg taagtatatt tgcttttttc gtatatttgg
      361 ttcatctttt tttgatgttt tacatattca caatgcagag gatttatacc agaggacatt
      421 agtcttgagt aagatactac aacatctcca actattttag ccagtctgac tacttgactt
      481 ggtaatattt gttgctaata atcatgtata aaataagctt atatacatag taacttcagc
      541 aaaggattta gtaatgatca aaatcatatc tcaatgtact gtttttttct ttaaaaaaac
      601 agtacattct attttataat gactgtgtat tcccatggaa ggagatcacc caattctccc
      661 ccccgacttt tgacgcagtg tcttgttaac agggggattg accatgctta ttggctatgt
      721 acgcgtatca acaaatgacc agaataccga tctacaacgt aatgcgttta tttgtgccgg
      781 aagtgagttg attgttgcgc gaatgggaag ttggccagaa gttgttatgg catagtgttt
      841 acggtcgttg atacaggtaa acaaaagcgg ggagagtcat actccctgaa tagtcgcgaa
      901 aatatctgtg gcattgtcaa attcgtgtcg gtgactaata ttttagtaat atgccattcg
      961 aaatggagtg ctaacttatt gatttaattg atgtgcgata ttgtatttta tcgtgttttg
     1021 ttgtctgttt tttatccagt aagtgttgtt atatgtatga aagataaggg atttatcatt
     1081 tttgaggtgt gttcatgaag caaacagttt atatcgccag ccctgagagc cagcaaattc
     1141 acgtctggaa cctgaatcat gaaggcgcac tgacgctgac acaggttgtc gatgtgccgg
     1201 ggcaggtgca gccgatggtg gtcagcccgg acaaacgtta tctctatgtt ggtgttcgcc
     1261 ctgagtttcg cgtcctggcg tatcgtattg ccccggacga tggcgcactg acctttgccg
     1321 cagagtctgt gctgccgggt agtccgacgc atatttccac cgatcaccag gggcagtttg
     1381 tctttgtagg ttcttacaat gcgggtaacg tgagcgtaac gcgtctggaa gatggcctgc
     1441 cagtgggcgt cgtcgatgta gtcgaggggc tggacggttg ccattccgcc aatatctcac
     1501 cggacaaccg tacgctgtgg gttccggcat taaagcagga tcgcatttgc ctgtttacgg
     1561 tcagcgatga tggtcatctg gtggcgcagg accctgcgga agtgactacc gttgaagggg
     1621 ccggcccgcg tcatatggta ttccatccaa acgaacaata tgcgtattgc gtcaatgagt
     1681 taaacagctc agtggatgtc tgggaactga aagatccgca cggcaatatc gaatgtgtcc
     1741 agacgctgga tatgatgccg gaaaacttct ccgacacccg ttgggcggct gatattcata
     1801 tcaccccgga tggtcgccat ttatacgcct gcgaccgtac cgccagtctg attaccgttt
     1861 tcagcgtttc ggaagatggc agcgtgttga gtaaagaagg cttccagcca acggaaaccc
     1921 agccgcgcgg cttcaatgtt gattacagcg gcaagtatct gattgccgct gggcaaaaat
     1981 ctcaccacat ctcggtatac gaaattgttg gcgagcaggg gctactgcat gaaaaaggcc
     2041 gctatgcggt cgggcaggga ccaatgtggg tggtggttaa cgcacactaa ccgctgattt
     2101 aaccggcgca gtctctcctg cgccggtgta ttaacttatc tcctgtaacg catgtctctg
     2161 gcgttcgacg atattggtcc acaaattgtc tttatcgtca gtccacaaat taatcagcaa
     2221 ggaaaaaaag cgttcagctg ccggagaaag cacggcatct ttgcggcgaa taatccccaa
     2281 tgtccgacgt ataacgggtt ccaccagtgg gataccaata atcgaagaat agggcgcatg
     2341 gggcattgcc agaccaggaa gcgccgaaat ccccagtcct gcctccacta gtcctaatga
     2401 cgtcgaaaga tgacgcactt cgtaaaacca gtccagcttc cagggcttgt cggctagctg
     2461 ttgttctatc aacaatcggt tgccgctgga ggaacgtacg ccaatcattt tgtaacccac
     2521 taactcctgc cattctacaa gctacttttt ggccaatgga tggtcacgcc gacaggccag
     2581 cacgaatggc tcgttgacca gtggggtaaa atcaatggat gaatttgtga cgttgttcat
     2641 gtttatgcca aagtctgatt cgttacacag caccgattcc atgcaattat tcgtgccttg
     2701 ttccagaatc cgcactttaa tattgggata cagctcatta aatttaccga ttgccagcgg
     2761 taaaaaataa aatactgcgg tcggaataca cgctaatgtc accatatcac gatgataagc
     2821 attcatatca cgaatattaa aaagcgtttc atcgaatttc tttattaatt cccgcgcctc
     2881 cgggagcaac ctttttcctg cttttgttaa cgtaaccttg cgagttgtac gctcaaatag
     2941 ttgaatatgt aaatcttcct ccatcttttt tattcttcgc gttaatgcag gttgcgtaat
     3001 attgagtaat ttagcggcat tgttaaatga actggactct gccagtatga caaatgcctt
     3061 catacttgat aattcatgct tcattattac tccggaaaat ggaagcgacg attttggggg
     3121 gctggtcgtt aaaaatttta actgcattta gccaacttaa attaatgaaa aaatgttatt
     3181 aatcgttgag ctaaagtcat tagagatgct ttgtccttaa tgtaaccata tcgcaataag
     3241 ttatgttttt aaattgaggg cattattatg aaaaaaatac cctgcgtgat gatgcgaggt
     3301 ggaacctcga gggccgcgtt cctgttagcg gaacatttac ccgaagatca aacgcagcgc
     3361 gataaaatat tgatggcaat tatgggttcc ggtaacgatc tggaaattga cggtattggc
     3421 ggcggtaatc cactgaccag taaagtcgcc attattagcc gttccagcga tccgcgtgct
     3481 gatgtcgatt atctgtttgc tcaagttatc gtccatgagc aacgtgtcga taccacgcct
     3541 aactgcggca atatgctgtc tggcgtaggg gcattcgcca ttgaaaatgg tttgattgca
     3601 gcgacttcgc cagttacccg tgtacgtatc cgcaacgtca atacgggtac gttcatcgaa
     3661 gctgatgtgc aaacgccaaa tggtgttgtc gagtacgagg gtagcgccag aattgacggc
     3721 gtaccgggta ctgccgcacc ggttgcgctc actttcctga atgccgctgg aacgaaaacc
     3781 ggaaaagttt tcccgactga taatcagatt gattattttg acgatgtccc ggtgacctgt
     3841 atcgatatgg cgatgccagt cgtcattatt ccggctgaat atctgggaaa aacaggttat
     3901 gaattaccgg cggaactgga tgccgacaaa gcattattag cccgcattga atctatccgg
     3961 ctacaagcgg gtaaagcaat gggcttaggt gatgtcagta atatggttat ccctaaacct
     4021 gtgcttattt ctccagcgca gaaaggcggg gcaattaatg tgcgttattt tatgccgcat
     4081 tcttgccatc gcgcgctggc gataaccggt gctattgcta tttccagtag ttgtgcattg
     4141 gaaggcaccg tcacccgaca aatcgtccct tctgtaggat atggcaatat caatattgaa
     4201 caccccagtg gtgcgctcga cgttcattta agtaatgaag gtcaggatgc cacgacgtta
     4261 cgcgcatctg ttattcggac aaccagaaaa atattttccg gtgaagttta tcttccctga
     4321 aaaaattcgt tgtcaggata aggacaatca ataaaggact tctgtatgag tcatacagaa
     4381 agaacaggat tttaaatgaa taagaaatcg ttatggaagc taattctgat attagcgatc
     4441 ccatgtatta ttggttttat gccagctccg gcaggattaa gcgaactggc gtgggtgctt
     4501 tttggtattt acctggcggc cattgtgggt ctggttatca agcctttccc ggaacctgtc
     4561 gtactgttaa ttgccgttgc tgcctcaatg gtggtggtcg gtaacttatc cgacggtgcg
     4621 tttaagacca ccgccgtatt aagcggttac tcttcaggta ctacctggct ggtgttctcg
     4681 gcgtttacct taagcgccgc atttgtgacc accggtttag gtaaacttat tgcctatctg
     4741 ctgattggta aaatcggtaa caccatgctg ggtctgggtt acgttacggt attcctcgat
     4801 ctggtactgg ctccggcaac accgtctaac accgcgcgtg cgggcggcat tgtgttaccg
     4861 atcatcaaca gcgtggcgat ggctttgggg tccgaaccgg aaaaaagtcc gcgtcgtgtc
     4921 ggacattacc tgatgatgtc catttacatg gtcaccaaaa ccaccagcta tatgttcttt
     4981 accgcaatgg cggggaacat tctggcgctg aaaatgatca acgacattct gcacctgcaa
     5041 attagctggg gtggatgggc gctggccgcc ggattgccgg gcatcattat gctgctggtc
     5101 accccgctgg taatttacac catgtatcca ccagaaatta aaaaggtgga taacaaaacc
     5161 atcgctaaag cgggccttgc cgaactggga ccgatgaaaa tccgcgaaaa aatgctgctc
     5221 ggtgtctttg tgctggcgct actgggctgg attttcagta agtctctggg ggttgatgaa
     5281 tccactgtgg caatcgttgt tatggcgacc atgctgctgc tgggtatcgt tacctgggaa
     5341 gacgtggtta aaaataaagg cggctggaat accttaatct ggtacggcgg tattatcggc
     5401 ttaagctcct tattatcgaa agttaaattt ttcgaatggt tagctgaagt ctttaaaaat
     5461 aacctggcat ttgatggtca cggtaacgtt gctttcttcg ttattatttt cctcagcatc
     5521 atcgtgcgtt atttcttcgc ttccggtagt gcctatatcg ttgctatgtt acctgtattt
     5581 gccatgctgg cgaacgtctc cggcgcaccg ttaatgttaa ccgcgctggc actgttgttc
     5641 tccaactcct atggcggcat ggttactcac tatggcggcg cggcaggtcc ggtcatcttt
     5701 ggcgtgggtt acaacgatat taaatcctgg tggttggtcg gtgcggtact gacgatatta
     5761 accttcttgg tgcatatcac cctcggcgtg tggtggtgga atatgctgat cggctggaac
     5821 atgctgtaaa tatactcgtc atacttcaag ttgcatgtgc tgcgtctgcg ttcgctcacc
     5881 ccagtcactt acttatgtaa gctcctgggg attcactcac ttgtcgcctt cctgcaactc
     5941 gaattattta gagtatatcc atttattatc tttctgcgca cttcacggtg cgcagatatc
     6001 tggagcattt gatgatcaag ttatctgaaa aaggcgtgtt tctcgccagt aataacgaaa
     6061 taattgccga agaacatttc accggcgaaa ttaaaaaaga agaagcaaaa aaaggcacta
     6121 ttgcctggtc tattctctct tctcataata cgtccggaaa tatggataaa cttaaaatta
     6181 agtttgattc attagcctct cacgatatta cctttgttgg tattgtacag accgctaaag
     6241 cgtccggaat ggaacgtttc ccgctgccgt atgtgctgac caactgccat aactcactct
     6301 gcgccgtcgg cggcaccatt aacggtgatg accatgtttt tggtttatcg gcagctcagc
     6361 gttatggcgg tatttttgtg cctccgcata ttgcggtcat ccatcaatat atgcgtgaga
     6421 tgatggctag cgggggcaaa atgatcctcg ggtcagacag ccacacccgt tacggtgcat
     6481 tagggacaat ggcagtcggt gagggtggcg gtgagttggt aaaacagctg cttaatgaca
     6541 cctgggatat cgactatccg ggcgtggttg cggtgcatct gaccggaaaa cctgcgccgt
     6601 atgtggggcc acaggatgtg gcgctggcta tcattggcgc ggtgttcaaa aacggttacg
     6661 tcaaaaacaa agtcatggag ttcgttggac cgggcgttag cgcgctctct accgatttcc
     6721 gtaacagcgt tgacgtgatg accacggaaa ccacctgttt aagctccgtc tggcaaactg
     6781 atgaagaagt gcataactgg ctggcgctgc acggtcgcgg ccaggattac tgccagctta
     6841 accctcaacc gatggcgtac tacgatggct gcatcagcgt tgatttaagc gccatcaaac
     6901 caatgattgc gctgccgttc cacccgagca acgtgtatga aatcgacaca ctgaaccaga
     6961 acctgaccga cattctgcgt gagattgaaa ttgagtccga acgcgtggcg cacggtaaag
     7021 ccaaactctc gctgctggat aaagtggaaa atggtcgcct gaaagtgcag caggggatta
     7081 tcgcgggctg ttctggcggt aactacgaaa acgtcatcgc ggcggcgaat gcactgcgcg
     7141 gtcaatcctg tggcaatgac accttctcgc tggcggttta cccgtcatca cagccggtgt
     7201 ttatggatct cgccaaaaaa ggtgtggtag cagatttgat tggcgcaggc gcaatcatca
     7261 gaaccgcatt ctgtggccca tgctttggcg cgggcgatac gccaatcaac aacggtttga
     7321 gtattcgcca caccacgcgt aacttcccga accgcgaagg ctctaagcca gccaatgggc
     7381 agatgtcagc ggtggcgttg atggacgctc gttctatcgc tgcgactgcg gcaaacggtg
     7441 gctatttaac ctctgccagc gaacttgatt gctgggacaa cgtgccggag tacgccttcg
     7501 atgtaacgcc gtataaaaac cgtgtttatc agggctttgt gaaaggggca actcagcaac
     7561 cgctgattta cggaccgaac attaaagact ggccggaatt gggtgcgctg actgacaata
     7621 tcgtcctgaa agtgtgctcg aagatcctcg acgaagtcac caccaccgac gaactgattc
     7681 cttccggtga aacctcttct tatcgttcaa atccgattgg tctggcgggg aatgtcagcg
     7741 agctgacaga ggtgtttgcg cgcattaagc agattgctgg tcaggagcat attgatccgc
     7801 tgcaaactga aattggcagc atggtctatg cggtgaaacc aggcgatggt tctgcgcgtg
     7861 aacaggcggc gagctgccag cgtgtgattg gcggtctggc gaatattgcc gaggagtacg
     7921 cgactaaacg ctaccgttct aacgtcatca actgggggat gttaccgctg cagatggcgg
     7981 aagtgccaac ctttgaagtg ggggattaca tttacatccc tggcattaaa gcggcgctgg
     8041 ataatccggg tacgacgttt aaaggttatg tgatccatga agatgcgccg gtaacggaaa
     8101 ttacgcttta catggaaagc ctgacggctg aagagcgcga gattatcaag gcgggtagtt
     8161 tgattaactt caataaaaac cgtcagatgt aaaaagcgcc atgtgaatgt aggtcgcatt
     8221 cggcacttat tgtcggatgc gatgctggcg catcttatcc gacctacaaa tcgcatcgaa
     8281 ccgtaggccg gataaggcgt ttacgccgca tccggcaaat agttaatagc tcttacttct
     8341 tcgcctctgc aaccactttg ctgcccacgc cgcggttatt gtattcccac atgcggttgt
     8401 agttagtgtc attcagattg cgctgtattt cgtcgttatc atctacgctg ccggtattac
     8461 ccgcaaacgg acgattagag atcaccgcat cggcccacgg tttagccgtg ttaaaacctt
     8521 cgttgatggc gctatcacgg atcaccacct gaccgttggt attagcatca acatccagcg
     8581 agcggcccag ttgcgccacg ccatcaccgg aagcattgaa acggctgttt acggcgagga
     8641 aaccgtaata gatgttagac agcgtagccg gtgcaaacac atatgcttct tgctgggtac
     8701 gtgagttcac cacgcggaat tcggtgttat cgaacaccac tgcgccgcga ccagaaacga
     8761 tatccacatc cccttcaata tagctgttgg tcaccagcgt acgcggctga cggttggttt
     8821 ccagacggtt ctgcacaccg ctgttggtga caaagaaggt gttctgacga ccgagaatgt
     8881 taacgttgtt aatctgtact tggtcaccgt cagtacgcag tgctaccgcc ggatggttac
     8941 ctgcatctac gctatcgccc agcgtgtttt cgatggtcag attttgcagt tgcagtccat
     9001 tgttttgtga ccagaagacc gcagaacaca gaacaccgat actgtcgctg cgtttgctct
     9061 ggcagctatc gtacatatac cacgctggtt tacctggcat atatttgccg cgcgggttga
     9121 cgtcgtgacg ccagtcggca gggctcatgc ccccatcaag ggaaagccca atcttcacat
     9181 caatcggttt ttcacctgta ccgtacagag tgattccacc tggagcagca gggacataca
     9241 ccgttccctg atactcccca ggcatcacgg caatatactg gcgcttgttg gtgcgcttga
     9301 taattgccgc atctaccgcc gcctgaatcg tggtatgcgt tacaccttga gtacccgccg
     9361 ggccgacaac aaagtcaggt tgcgcaggca gggttatcgg ggacggattc cacgctgcag
     9421 cacctggtgt cagggatgca aaatagtgtt gcgcatcgaa attcagcgct tcttttgccg
     9481 acagaatcgg gcgagaagag gtaccaggcg cagtttgatc agaaggacgt tgatcgggtg
     9541 gtgttgagct acaggcggtc agcgtcacgc caaaagccaa tgccagcgcc agacgggaaa
     9601 ctgaaaatgt gttcacaggt tgctccgggc tatgaaatag aaaaatgaat ccgttgaagc
     9661 ctgctttttt atactaagtt ggcattataa aaaagcattg cttatcaatt tgttgcaacg
     9721 aacaggtcat tatcagtcaa aataaaatca ttatttgatt tcaattttgt cccactccct
     9781 gcctctgtca tcacgatact gtgatgccat ggtgtccgac ttgtgcccga gaagatgttg
     9841 agcaaactta tcgcttatct gcttctcata gagtcttgca gacaaactgc gcaactcgtg
     9901 aaaggtaggt ggatcccctt cgaaggaaag acctgatgct tttcgtgcgc gcataaaata
     9961 ccttgatact gtgccggatg aaagcggttc acgacgagta gatgcaatta tggtttctcc
    10021 gccaagaatc tctttgcatt tatcaagtgt ttccttcatt gatatcccga gagcatcaac
    10081 atgcaatgtt gtagggatgg caatttttac gcctgttttg ctttgctcga cataaagata
    10141 tccatctacg atatcagacc acttcatttc gcataaatca ccaactcgtt gcccggtaac
    10201 aacagccagt tccattgcaa gtctgagcca acatggtgat gattctgctg cttgataaat
    10261 tttcaggtat tcgtcagccg taagtcttga tctccttacc tctgattttg ctgcgcgagt
    10321 ggcagcgacc gggtttgttg ttatatggcc ttcagctatt gcctctcgga atgcatcgct
    10381 cagtgttgat ctgattaact tggctgatgc cgccttgccc tcgtctatgt atccattgag
    10441 cattgccgca atttcttttg tggtgatgtc ttcaagtgga gcatcaggca gccccctcct
    10501 tattgcttta attttgctca tgtaatttat gagtgtcttc tgcttgattc ctctgctggc
    10561 gaggattttt tcgtagcgat caagccatga atgtaacgta acagaattat cactgttgat
    10621 tctcgctgtc agaggcttgt gtttgtgtcc tgaaaataac tcaatgttgg cctgtatagc
    10681 ttcagt
//
LOCUS       DAEEGA010000115        10126 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEEGA010000115 DAEEGA010000000
VERSION     DAEEGA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.115"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..179)
                     /locus_tag="KLH46_003490"
     CDS             complement(<1..179)
                     /locus_tag="KLH46_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBE0380047.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKKVPRV"
     gene            complement(382..849)
                     /locus_tag="KLH46_003491"
     CDS             complement(382..849)
                     /locus_tag="KLH46_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBE0380048.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRTQCLK"
     gene            complement(846..1379)
                     /locus_tag="KLH46_003492"
     CDS             complement(846..1379)
                     /locus_tag="KLH46_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBE0380049.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(1445..2074)
                     /locus_tag="KLH46_003493"
     CDS             complement(1445..2074)
                     /locus_tag="KLH46_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380050.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNGAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(2078..2293)
                     /locus_tag="KLH46_003494"
     CDS             complement(2078..2293)
                     /locus_tag="KLH46_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBE0380051.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(2586..2660)
                     /locus_tag="KLH46_003495"
     tRNA            complement(2586..2660)
                     /locus_tag="KLH46_003495"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2625..2627),aa:Gly,seq:tcc)
     gene            complement(2664..2739)
                     /locus_tag="KLH46_003496"
     tRNA            complement(2664..2739)
                     /locus_tag="KLH46_003496"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2704..2706),aa:Thr,seq:tgt)
     gene            complement(2742..2818)
                     /locus_tag="KLH46_003497"
     tRNA            complement(2742..2818)
                     /locus_tag="KLH46_003497"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2782..2784),aa:Arg,seq:tct)
     gene            complement(2820..2896)
                     /locus_tag="KLH46_003498"
     tRNA            complement(2820..2896)
                     /locus_tag="KLH46_003498"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2860..2862),aa:Ile,seq:cat)
     gene            complement(3096..3476)
                     /locus_tag="KLH46_003499"
     CDS             complement(3096..3476)
                     /locus_tag="KLH46_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBE0380052.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(3469..3669)
                     /locus_tag="KLH46_003500"
     CDS             complement(3469..3669)
                     /locus_tag="KLH46_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HBE0380053.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(3764..4021)
                     /locus_tag="KLH46_003501"
     CDS             complement(3764..4021)
                     /locus_tag="KLH46_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380054.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(4018..5409)
                     /locus_tag="KLH46_003502"
     CDS             complement(4018..5409)
                     /locus_tag="KLH46_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBE0380055.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(5406..6284)
                     /locus_tag="KLH46_003503"
     CDS             complement(5406..6284)
                     /locus_tag="KLH46_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE0380056.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(6295..7203)
                     /locus_tag="KLH46_003504"
     CDS             complement(6295..7203)
                     /locus_tag="KLH46_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBE0380057.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(7190..7423)
                     /locus_tag="KLH46_003505"
     CDS             complement(7190..7423)
                     /locus_tag="KLH46_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380058.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(7420..8436)
                     /locus_tag="KLH46_003506"
                     /pseudo
     CDS             complement(7420..8436)
                     /locus_tag="KLH46_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            8368..8631
                     /locus_tag="KLH46_003507"
     CDS             8368..8631
                     /locus_tag="KLH46_003507"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE0380059.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            8621..8929
                     /locus_tag="KLH46_003508"
     CDS             8621..8929
                     /locus_tag="KLH46_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0380060.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(8971..9147)
                     /locus_tag="KLH46_003509"
     CDS             complement(8971..9147)
                     /locus_tag="KLH46_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HBE0380061.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTT
                     HLWDIRWLRHRARK"
     gene            complement(9144..9554)
                     /locus_tag="KLH46_003510"
     CDS             complement(9144..9554)
                     /locus_tag="KLH46_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HBE0380062.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(9551..9829)
                     /locus_tag="KLH46_003511"
     CDS             complement(9551..9829)
                     /locus_tag="KLH46_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380063.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(9904..>10126)
                     /locus_tag="KLH46_003512"
     CDS             complement(9904..>10126)
                     /locus_tag="KLH46_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380064.1"
                     /translation="SGGQIQCATSPASVLMLEKQRNGEGWEGGVPLFLDEQSHQFLQL
                     ESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         2450 a         2716 c         2324 g         2636 t
ORIGIN      
        1 accctgggta ctttcttcat gaaactgcgg tgttcgagtt tgcgatattt cttacagggg
       61 aacgcaagcc cctccgcctc cgctttggat ttccgatcag cattaatgta atccaccatc
      121 tcaagactgc tcatggtcgg gtattcgcca gaaggaagaa cggttaatga tttgttcatg
      181 tcggtatttc ctttagaaag ttgagcctgt tcgcacagaa aagccgtccc cgagaggtcc
      241 tcacctatac ggcagttctc aggctcagct ttctgaaaga ctcgggattg ttacgcgctg
      301 cgatgcgcgg tttactgcgg acatataaaa gccccgcaag tgcgaggctc attaaatgga
      361 ctttgtgatt tgcaaaaaaa tttatttcag gcactgagtc ctgatgtact cctgaagcat
      421 tctcaatgct gtctggtcgc tgatgattcc gtcccggata ccgagaacgt ttcgtccagc
      481 aactggagag agttcgacgg tggcatcatt gcccatgccg gaggcgctgg aggtttcggc
      541 tgaggatggc acagggcatt ttcctttgac gagcacccga ccaccattat caagcttgcg
      601 ccgaagagca tcattttcag ctttcgcatc agctaactcc ttcgtgtatt tagcatcgag
      661 tgcatcagca tcacgctggc gctgctgcat gtcagtaatg gttgcgttcg ccagcttcag
      721 ttctccggcg tttttgtcgc gctgctcttt gtaggtaatg gcgttatcac ggtaatgatt
      781 aacagcccat gacaggcaga cgatgatgca gataaccaga gcggagataa tcgcggtgac
      841 tctgctcatt gatctatccc ccaacaggct aatgcgcttt cctggtcacg acgaataacc
      901 tgtccatagc agttatttga acgtatgcgg caatcgcgcc caacatcttt tatccaccag
      961 cgaatcgcct cgcatgcgcc cttacgatca ccggcattca gccgcttata aaacgtcgac
     1021 gggaaacact taccggggcc aatgttatag gggcaaaatg acgctatacc cgctttctgg
     1081 ggttcggtca gaggtacttt aatattgcgc tccacccatg ccagcgcctt atcacgttca
     1141 atagcgttaa cctggtcaca tttttccttc gacagcttca ttcccggtat gacaggctta
     1201 ccatccacca ttgtggcacc acgacagatg gtccatatgc cagaaccatc acggtatgcc
     1261 gttgtgtgat taccctcttt ttcgttcaga aactggtcaa gtatctgggg agcagacgcg
     1321 cctgcggcaa tcagcgccag aacagcagcc gacaggccgt atttgatttt tgcgctcatg
     1381 gatatttatc aggatttatc ggcaacaaat aacgagccaa cttatatacg ccttttaaga
     1441 taagtcagcc ccggctggat ccagtcatca ggctctttct taaaggggga gtattgaaat
     1501 cacgaagaag agcctcccgc accgcagcat ccatgtctgc accactggca agtgcttcaa
     1561 tctctgccgc cacctgcaaa taccccatgc aacggccaat gcgtttcata actctggtgt
     1621 tttcgtcttt caggcatctg atagcgagat taataatttt caggcagtga tgatgcgatg
     1681 cccggacagg ttttccgttc tcataaacga cgaactcacc ctcttcacag gaaaccctga
     1741 aaggccgatg gggttcctgg tctttatccg gtgcagacgc aataaaatca cttttatgct
     1801 cttcctctcc ggcaccgtta accgtaatga cgtactcagg ccgcaggtaa gccgcctctt
     1861 tttctgacaa tgcggccggt aaaaactttt cctgcaaaaa cttcataaaa gtctcgtgag
     1921 cgactttgtg gcagtaatcg taaatcgccg cataatgctc atcgcggcgt ttgttttcat
     1981 cttcagaact caccaacgcc gacaattttt tattaagctc agtgatttca ttttccaggt
     2041 gattgaatcg ctgattcatt tcttcatgct tcattattta ctctccccgt gcagccttac
     2101 gcttgtcttc tcttattttg aaatacagat tcgtcagata agtcagaagc cccagaagca
     2161 gacttcccag cacaccaatc gcagcccact gtgatggcct gacctgatcc aaccactgca
     2221 aaaaccagta gccggcactg ccggcggagg tgccgtaggc aatgcccgtt gaaattttgt
     2281 ccatggattt catagcctca cctccgcaaa taacggatgg cgtagtttta cactgagaaa
     2341 tgaaagggat ttgaaaagaa aaaaacgcaa aagcgggcga aacgatatat acagtaagga
     2401 aagcactcta tccaacaaac cacccacagt taatcggaat aaaagcagag tgcttatgaa
     2461 tgatcgcacg cccgaaggtt agtatttctg cacagcaatt ttgcaaaaaa aggcgatcat
     2521 tcataactta aacgtctttc agtcactccg ggatttccca tcatcgcaga ctgaaagact
     2581 ctgactggag cgggcagcgg gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat
     2641 agccattata cgatgcccgc atatggtgcc gactaccgga atcgaactgg tgacctactg
     2701 attacaagtc agttgctctg cctgctgagc taagtcggcg ctggcccacc accgaggact
     2761 cgaacctcgc accgtcaact tagaaggttg atgctctatc cggatgagct agtggtggtt
     2821 ggtggccctt gctggacttg aaccagcgac ctggcgatta tgagtcgctc gctctgacca
     2881 actgagctaa agggccggag gcagaataat aaccatatgt catcacatct gcaaactcat
     2941 ctgaccacca gcgtgtttaa cgtcctgtac cgtttttcag gcataaaaaa cccgcccgga
     3001 ggcgggttta agctgtgtgg cgaagcaacc actcttaaca gaatatcccg ttttttacgt
     3061 acgtaaaata ttttctagaa agtcgcccct taccatcagg gatgttcaat atatttgtcc
     3121 atttctaacc ggacacccaa catcatcaac attccttcca ccaccccctc ggctttttgt
     3181 agcttctttc ctatataacc atctgagcat ccatgcttcc gcgccagcgt catgaatgtc
     3241 atcccaaaca cgtagtagtc aaccagcaag tcatgtagat cgttgttgtt ccggttaagg
     3301 cgggccatac acccacatat aaccatcgcg tcatcgtcac agcattgcgg gcgggatttt
     3361 acttttgaag ggattaatcc cttaaaaccg gcagcaatgg acgaccaggt cacatcctca
     3421 tgattattag ccacccacgc gccccagcgc tcaagaacct gctgaatatc acgcatcact
     3481 gtctttaccc ctgtcccatc cacgatgaac aatcagaaca ccatcaacaa tggcgtgccc
     3541 tttgccctct ttatcttcag catattttct taccgtggcg cgattacagt tcagcattcg
     3601 tgaaacttcg gtcatattac ctcgtgtctg gataagcagt tccggtatcg tttgaatttt
     3661 gacgctcatc aaatactctc cggttcggtg atttttatcc cgggctttcc accagggaca
     3721 taatgatcgc gcacaagcca gggcaaaaca aactcatggc gcatcagcgc agcgcctcct
     3781 gcaccagttt ttcaaacttt ccgactctgg tttccagctc tgccacacag tccaccagct
     3841 catctactgc tttctgtgcg cgatgcttcg cctgcatcag ttcccgaagc gcgggtacca
     3901 tatctttacg gatagcatct tttgttatgc ccgttttttc gagttgttca gcatgacgca
     3961 gcatttcctg cgcgtgttta cgcaattgtt caggggtaaa agtcattgtc tggttgttca
     4021 aaagaaacgc tccatcttac tgctgtcagt tcgtttatta ctgtatctgc gcggattgcc
     4081 gggcttcatg ggagtggaaa gcacccgtgc actttcctgg tccacaggca gaaaatgtcc
     4141 gttataaaaa cgccggtaaa tcgttcccag agaaccgtta cgttgtttcg tgatattgat
     4201 ttctgcgatg cccctggcct gcgtatccgg gttgtacact tcatccctgt aaagcatcag
     4261 aatgatgtct gcatccgcct ctatttctcc ggaatttttc aggtctgagt tcatgggacg
     4321 tttattgggt ctggactcca caccgcggga gagctggctc agcgcaatca acggaaaacc
     4381 accggatttt gccaggcctt taagcccctt tgagatttca cccacggcaa ggtcatgacg
     4441 ccccgtggtt cgggttttta tcagcccgag ataatcaacc accaccagcg ccgtttccgg
     4501 atatttaatc aggtggtgtt tcgttgttgc gcatatctca tcaatggcca ggttcgcctg
     4561 gtccaccatc cagatattgc gcccggtcat ccgccccacc ccttgtgaga aacgcgtcca
     4621 gtcttcatct tcaaagtgag ccacagattt caggcgtgat actggcatcc ctccagccgc
     4681 agacaccata cgttcaccaa tctggatgtt cgccatctcc atggtgaaca gaagcacacc
     4741 atgcccctgc tcagtcacct tgtcgatgat gtccagcgca agttcggttt tccccatcga
     4801 aggacgggcg gcaatgaata ccaggtctcc gggctccata ccgcctgttt ttgcgtccag
     4861 ttcatcaata ccggtcatca acgtcctgga tttctccagc ccctgattcc ggcattcaac
     4921 acgctcaacc acttccggaa gcacatcatc aatatgtacc ggctgaatga cgccctttcc
     4981 tgtcgacagt gtgaccatca tgttctgcgc atccttcagg gcatcttcag ctgcttcaca
     5041 ggtatgcgca tcacgtaatt tctgcagcgc ctcattcagt gttttttctg catcgcgcag
     5101 tgcggcattg cgccgcaacg ctgcaacata gtgctccagt gaagacttca cccaggtttt
     5161 acgcccggta tcagtaatca ccggggcaag ttccggcatc tcattacaca acagcacggg
     5221 gtcaatcact cctgaaacac gggcctgtct gcagatgcct gtatagatat cctgatacgc
     5281 tcgtacagaa aaaacgtccg ctggcagtgt ggccagaata tccatcactt caggatctgc
     5341 tccgcgcaga aagaacgcgc cgatgacagc gccttcaagg tcatcgttac gccatactgg
     5401 agttgtcatg cagccacacc tctgatacga gaacggtaac tgggccagtt aaacgacaac
     5461 cagttgcgtc ccccgtctgt gatcctgtcg gcaattcggg gactgatgaa cgcccacaac
     5521 tcttccggtg agaggttact gataagaatg gtgggcaaga tactttcgta cctggcgttg
     5581 ataatttcct gcaaaatagc catttcagcc gcgctgccaa actgaacgcc aacttcgtcg
     5641 atgatcagca aatccattga cgcataacgc tcaataactt catccgctgt tttttcgctg
     5701 tcattccgcc agcaattttt cacagcacgg gtaaggcgca tcacgtcggt gatctccaca
     5761 ctggccagat agttacggat gatgtgtttt gccatagcca cagccagatg attttttccg
     5821 gtaccacaac tgccggtcat aacaagactg gtaccgttct ccagcatatc tggccagttc
     5881 tccgcatagc ggcgacaggc cgcaagattt ctggctgcgc caggattaac ctccagataa
     5941 ttatcaaact cgcagtcccg aaaacgcagg gcaattccgg cgttatcagt cagctcttcc
     6001 gccttgatgg acgacagctc catggtcaaa tcgttggcct cagcgattaa gcagtcaggg
     6061 cagcatgaaa tttcttctct gtcctcgcca ttacgatcgc tccacaccag tatatgcgtg
     6121 tgatattcgc catgtttttc gcaatatccg cgaccttcac gcatcaggca ggaacgataa
     6181 ggccatggct tttcgccctt ctgagcaaat gcaatctctg cccgtaactc atccattcgc
     6241 gcctgtagtc ttttttgttg ttcacgcagg ttaaacgtca tcatcgctgt cacctcagaa
     6301 tgtcagtttg tcactggatt taccgaattt gtcagacatg gctcccaggc cagccaggac
     6361 atcgacctgt cgctgtcgcc cacctctggg agcggctggc tgttgccaga agtcttcgaa
     6421 gtgacgatcg ggtccaaaga acgtcgcagc ctgcttcacg aactgtgtgc cggtatttcc
     6481 tgtggcacgt acccaggcag catagcgttt cacgccatca agcatggctt caggttttat
     6541 tccctccctg agccgggctt tccaggcttt gaaggctgcg gattttgagt taccaccagc
     6601 acgttttgga tattcctgcc aggcctgttc aaattccggt gaatattcct gtcgggaaga
     6661 acgcgctggt gcagacgcgt cagcggatgc gccaatagtg tttttactct ctgtagtatt
     6721 ctctgaagta atctctgttg tattctctgt aagatcgaaa ttggttttcc cttctccgcg
     6781 gcgaggggtt tcccgtgtcc gcggtgaagg ctttccctcc tccgcgaaat tgggttttac
     6841 agtttcccga aaacgggttt ccccatttcg ggaaaactga ttgttttcat tgataatttc
     6901 attaaggcgc tcacaatcta tacggtagaa cattttgtgc tcaagacgct tgttggtttc
     6961 aaccaaaatg cctctggaca caagatgctt acgcgctaca gcctgttgtt caaatgtaag
     7021 tccggtttcg tgttgtatct cttcacgcgt tttatgtacg ccttccgctg catgtgcttt
     7081 atcctgccag taaaaaatct gaccaaagaa aataacagcg tgcggacttc ccatgtattt
     7141 aacgagccca gggtaataag caaccggatg tccaaaatcg agcagaagat cagacggacg
     7201 catagccacc tcccaggcgt ttaaacattt ttccgggctg aaacgccacc agcggataac
     7261 tcagggtatg agtacgtccc tgaacctggc agacaacctt ctggctttcg gtactgacca
     7321 ggcaaacccg cagaacgtag ccgttgctgg tggtgaacca ctgccccaca cgggggcaat
     7381 ggttgtatcg gtgatacagg aaattaacga tgtggcggat catgggcgca cctccttgtc
     7441 agaaccattc agcctggaat caacaagtgc agcaccaaaa acagcatcac ctacacggtc
     7501 gtacagtttg ccagccagcg gagattcaac ggccttaagc attgggtaaa gctggcttgt
     7561 ccagatttga tggatttcac gcaaatgcag gtatacgcct ctggcgtttt gtgcgacaga
     7621 tgacatgtca gccgcaccag ctcctgataa gctcctctcc atctggttaa aggcattgat
     7681 gtatgcctct ttgaaccggg cggcacgttt accagtgaag cccatagcaa ggaaggcgaa
     7741 gccgccgcgg gtaatttggt agcagggaag tttgcgagta ccgccgttgg gctggcgtac
     7801 caaaattgat gtctccgcaa aattgcgggc acaaaactct ggagaacaat ccaaaatgcg
     7861 gatctttttc agaacatcgt catgacgttt agagaagaag ttggcaacag ccagggatga
     7921 agtaacagcc taaccatcaa cgatggcaat ttcaggttga gagagggttg ggagagtagt
     7981 catggtgaca gcccctatgt tgaattcaaa gaactcacca catgggacgc caatcacaga
     8041 ggtggtgaga cgtacagggt tggcgtaacc ggtcaacata gaacccggcg catcttgcga
     8101 tgtccctgca cgccccacca taatttgggc gtagcaatgc tcatgacacg aaaaaaccgc
     8161 atgagcgcgg ttatgctcta tattgaattt caggacgcca atcccggccc ccgctttata
     8221 aggtgcagag acagtgtaac gtcccgaaat tgcagaatca atatattgtg cagatataac
     8281 gattttgtta tgttttgctg tatgaactac actatcgaat actacagtga agaggtccga
     8341 ctggaggtcg atcagcttcc attgagtatg cgtgccagat accagcatct tgttgaacgc
     8401 atgaaggtat acggcagcaa tctcggagaa cctcacacca gcgcctttgg tgacgggctt
     8461 ttcgaactca gaattaaagg caacgatggg atcgcgcgtg ttttttactg cactctgaca
     8521 gggaaacgca tcatcatgct gcatagtttt gtaaagaaag cgcagaaaac accgccagcc
     8581 gaacgcaaga aagctgaaac cagaatgaag gaggttaagc atgactggta aacgcactct
     8641 ccccaccatg acacacgacg aaatggcagc caaatggatg gaagacccgg cttttaaagc
     8701 agaatacgac gctatcgctg acgaattcgc actgcttgat gaaatgctgg cagcacgcaa
     8761 agaagctggc ttaactcagg ctgaagttgc cgagcgaatg ggaacaaaag cgaccgcgat
     8821 caccagaatg gaaagtaatc tcgcatcagg tatcagcggc ccatcatttg ccacactaaa
     8881 aaaattcgcc cgcgctactg gaaaaaaact ccagatccgc ttcgtttaac cacaccgcgc
     8941 cgtcattctg gcggcgcacc ggataaatag tcatttcctc gcacgatgtc ttagccaccg
     9001 gatatcccac aggtgagtcg tgtagttgaa ggtttttacg tcagattctt ttgggattgg
     9061 cttgcgttta tttctggagc gtttcgttgg aaggtatttg cagttttcgc agatgatgtc
     9121 ggtgaaactt cgtcgatgtc gcctcattca tacctcctgt cggtaaatct gacaccctga
     9181 ccaatagccc aggctgttgt gtactcgatc agacttgcca tgcgcttcac gctcatctgc
     9241 gcgctgcttt cgcgaatgtt gacgcattct ccttcaaggc cgggcaaaac atcagcttcc
     9301 tgttttgttg ccactgcatg accgctgatc aacaaaacct tccattgttc tggttttaac
     9361 catttgccgc accattgaac ctgacgagcg atatccgcca gcatcgcgtg aaattttgcg
     9421 ttctggtcaa gatttcgctt gtagtcagta atgcggatgg tgactggctt gtctttatcg
     9481 agaggagttg agaggatggc atttattgcg gcttgctgtt gttgcttagt tcggaggaag
     9541 attgtttgct tcatcgaaaa ttcttctctt taattccagc ggctctgata gctttcatta
     9601 ctgcaattac cgttttgtcc ttcccatcct catgccccat cgcataagca ccttcttcac
     9661 catctttcca aaagtcgtca ttcgattcgg gccagtcgat atccagttca atagctgctc
     9721 gcgatgcctg ccatatcacc caggcaaact cttttaattc atcgtctcct gtgaactggc
     9781 ttttgtcttt tgaccaccag ttttcaaact gtcggtagct atcgttcact tccctctccc
     9841 ccaaataaaa aggcctgcga ttaccagcag gcctgctatt agctcagtga tgtagatggt
     9901 catttaatac tccgtcacgt tttcctgtcg ccacgcctcg tcatattccg atttcggcat
     9961 attggcgatg tagctatatg gcgatcctga ttcaagttgc aggaactggt gcgattgctc
    10021 gtcaaggaac aacggaacac caccttccca accttcgccg ttacgttgtt tttcaagcat
    10081 caaaacagat gccggtgaag tggcacactg aatttggcca cctgaa
//
LOCUS       DAEEGA010000116         9951 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEEGA010000116 DAEEGA010000000
VERSION     DAEEGA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.116"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="KLH46_003513"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="KLH46_003513"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBE0380065.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISKSVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="KLH46_003514"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="KLH46_003514"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBE0380066.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="KLH46_003515"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="KLH46_003515"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBE0380067.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="KLH46_003516"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="KLH46_003516"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBE0380068.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIDAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAV
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4818
                     /gene="citX"
                     /locus_tag="KLH46_003517"
     CDS             4267..4818
                     /gene="citX"
                     /locus_tag="KLH46_003517"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBE0380069.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4793..5671
                     /gene="citG"
                     /locus_tag="KLH46_003518"
     CDS             4793..5671
                     /gene="citG"
                     /locus_tag="KLH46_003518"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBE0380070.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5722..7184
                     /gene="citT"
                     /locus_tag="KLH46_003519"
                     /pseudo
     CDS             5722..7184
                     /gene="citT"
                     /locus_tag="KLH46_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7298..8104
                     /gene="rna"
                     /locus_tag="KLH46_003520"
     CDS             7298..8104
                     /gene="rna"
                     /locus_tag="KLH46_003520"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBE0380071.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8333..8743
                     /gene="rnk"
                     /locus_tag="KLH46_003521"
     CDS             8333..8743
                     /gene="rnk"
                     /locus_tag="KLH46_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBE0380072.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8974..>9951)
                     /locus_tag="KLH46_003522"
     CDS             complement(8974..>9951)
                     /locus_tag="KLH46_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBE0380073.1"
                     /translation="IACGDCFFCRLQQYAACENTNAGKGAALNKKQIPAPAALFGYSH
                     LYGGVPGGQAEYVRVPKGNVGPFKVPPLLSDDKALFLSDILPTAWQAAKNVQIQQGSS
                     VAVYGAGPVGLLTIACARLLGAEQIFVVDHHPYRLRFAADRYGAIPINFDEDSDPAQS
                     IIEQTAGHRGVDAVIDAVGFEAKGSTTETVLTNLKLEGSSGKALRQCIAAVRRGGIVS
                     VPGVYAGFIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEEIVTHYMP
                     FEEAARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2350 a         2677 c         2505 g         2419 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt aaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatgaacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcgacgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg tgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ctacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4741 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4801 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4861 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4921 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4981 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     5041 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5101 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5161 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5221 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5281 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5341 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5401 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5461 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5521 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5581 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5641 gatccttacc tggtttttag cacagattta attatttaag cacttgataa atttggaaat
     5701 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5761 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5821 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5881 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5941 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     6001 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6061 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6121 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6181 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6241 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6301 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6361 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6421 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6481 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6541 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6601 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6661 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6721 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6781 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag
     6841 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6901 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6961 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     7021 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7081 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7141 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7201 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7261 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7321 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7381 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7441 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7501 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7561 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7621 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7681 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7741 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7801 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7861 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7921 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7981 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     8041 cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8101 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8161 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8221 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8281 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8341 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8401 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8461 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8521 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8581 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8641 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8701 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8761 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8821 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8881 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8941 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     9001 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9061 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9121 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9181 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9241 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9301 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9361 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9421 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9481 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9541 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9601 ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg aaccttgttg
     9661 gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9721 tttatcatct gaaagcaaag gtggtacttt aaacggcccc acattccctt tcgggacgcg
     9781 gacatattcc gcctgcccgc caggaacgcc accatacagg tgactataac caaacaatgc
     9841 cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9901 gcaggcggca tattgctgta atcgacagaa aaaacagtcg ccacaagcaa t
//
LOCUS       DAEEGA010000117         9927 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEEGA010000117 DAEEGA010000000
VERSION     DAEEGA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9927
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.117"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..338
                     /locus_tag="KLH46_003523"
     CDS             <1..338
                     /locus_tag="KLH46_003523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380074.1"
                     /translation="SSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLNA
                     RNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYNI
                     DPLCECFKY"
     gene            526..1641
                     /locus_tag="KLH46_003524"
     CDS             526..1641
                     /locus_tag="KLH46_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380075.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2320..2538
                     /locus_tag="KLH46_003525"
     CDS             2320..2538
                     /locus_tag="KLH46_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBE0380076.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2756..2998
                     /locus_tag="KLH46_003526"
     CDS             2756..2998
                     /locus_tag="KLH46_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380077.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3328..4257)
                     /gene="fdhE"
                     /locus_tag="KLH46_003527"
     CDS             complement(3328..4257)
                     /gene="fdhE"
                     /locus_tag="KLH46_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBE0380078.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4254..4889)
                     /gene="fdoI"
                     /locus_tag="KLH46_003528"
     CDS             complement(4254..4889)
                     /gene="fdoI"
                     /locus_tag="KLH46_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBE0380079.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4886..5788)
                     /gene="fdoH"
                     /locus_tag="KLH46_003529"
     CDS             complement(4886..5788)
                     /gene="fdoH"
                     /locus_tag="KLH46_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBE0380080.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5801..8851)
                     /gene="fdnG"
                     /locus_tag="KLH46_003530"
     CDS             complement(5801..8851)
                     /gene="fdnG"
                     /locus_tag="KLH46_003530"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8264..8266),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBE0380081.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9045..9878
                     /gene="fdhD"
                     /locus_tag="KLH46_003531"
     CDS             9045..9878
                     /gene="fdhD"
                     /locus_tag="KLH46_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBE0380082.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2490 a         2395 c         2543 g         2499 t
ORIGIN      
        1 tgtcgagtaa taatgaatat gacctgttta aaaatagaga aacctcactg tggaaacgca
       61 aagtaccaga aagcgcagcg ctggcaatga cgggaacagt atataaatta tttggtaatc
      121 gcttactcaa cgcccgcaat cagttagata aaaatctcct cgaaatggct atgcaaatgg
      181 tttatggcaa agatatggtg acaaagtccg gggatatatt gattgatata caattaaata
      241 aggatggcag tctccagagt aagcactaca cacttgatgt aggtgaagtt ataaacagtt
      301 ataatattga ccctctttgt gaatgcttca agtattaatc aaacttactt acatgataaa
      361 aatcctggtg acgttattag cctgtatggc actttagata acattcccct tgtagttaag
      421 gaatgcacgg gtaaaaatca tcaaattaat gttaaagcca aattgccaca agaacgaagt
      481 gaaaaagttg aggatatgtg tgaaagaatg aaaggtggca tctctatgtt taaccacacc
      541 acaaaaacag ctgataatat agagcataat ttgcagctaa gttttctcgt gggttcacgt
      601 ccgtcgatca ccaataaata ctcagcaaaa gatgtaggtc cagatatact tgcattgccc
      661 gtcaacgtat atcatgcgga ttttaaaatt aaaatcaata atgcagtgga gaaggcttca
      721 tcaattgtgt tatgtgctgt gcaaaaaaat ctccatgcag caaggaaggt tcttagcggg
      781 ataataggaa atgtgccaaa aaataataaa ccaaaaacaa ttatttttga tttgcgtttt
      841 cttagtgcaa ttaagcagaa agtttttttg ggcagtgaaa aaccgttgtt tgtaaagcat
      901 accgcaatga tgagggaggc atgtaaggaa ttaccgcagt cagtggaata tgttccttta
      961 gcctgtgaag cgcatagcca tcgcagcgtc gcccttgcca aaaccgtggc tgcgcccatc
     1021 atggcgacag gtgtgggctt ggcattaatg tatgcaacat catcaggacc gtattatgcg
     1081 caaagtcttt ctggttcgcc ggatatcgag atgataaaaa atcgcatggc tgaattgttt
     1141 aaccagtcgg atatttctgt gcgcaacaaa tatagcccgg cttttgataa atggaatgaa
     1201 ctggtagata aattaaatca cgaaagaaat acagttccgt ttgcgtgttt gaccaccatt
     1261 ttagcgacgg cgataaatga aactcttgag ggggatacga atgttgccgt tgtgatgggg
     1321 tgtaaatctg cgaaagatcg cacgatatcc attgtccttg gcaactcgat gttgcaaaca
     1381 ctccttgaaa aacgcctcgc cgatagtgga gaaatagaaa atctttttga tcaacaggga
     1441 tattttagtt gcgattcatt aacacccgaa gaattaatga tgttaaaaga cctttttgat
     1501 attcgagttc ttcatgtctc taacaaattc aatgttggat tgcaaggtaa tatcaacact
     1561 gatgttttac aagattcgtt ttttaaaaat gtcgatttca ttcatgaata taactcgtat
     1621 accgtaggga tgggagtctg acgagtaaaa ctcattagga ttatcagaat tcggacaatg
     1681 cattgcaaaa atgacataca aagagagatg tgtgcaggta cttagccgcg aggggcatgg
     1741 cgaagccata cagaaagtac cagtattcag aactccagtg aaagcggcat agtggccgcc
     1801 tgctcaggag ttttgctatg acccgtaatc gtgctgaacg tcgccaccct atgatgcgat
     1861 taaaaaaact catcgtcgcg ctgatgattg acgtcaatgc tatcggatga tgccgggggt
     1921 gttccggaag gaattccttg tttcccgaac tgttaccaac aaaattataa cgatgagcaa
     1981 gtaaagaccg gttggtggca gtactgaccg gtctgtgatg aaagattaat ttcaccttaa
     2041 atcctggtgc tgttaggatg ctgacgtttt tcggctacac cattcagatt atgcaaataa
     2101 atatccctga atataatata ttcatagcac ccataactca tcctgggcga taactaaacc
     2161 atttttgagt aataaatgaa acaactgcaa cgctcttatt atttctggaa aacgcctcgc
     2221 aaaaacaaaa tgacatgcaa aaatccgcat ttttttaaat gcttttccgg cgtaattttc
     2281 gcacatacat taatgagtaa tatatgtaag gtggtggtaa tggcgatgaa tacggttttt
     2341 cttcatttat cagaagaggc aattaaacgg ctgaacaagc ttcggggctg gcgtaaggtt
     2401 tcacgatctg caattttacg cgaagcggtt gagcaatatc tggagcggca gcaatttccg
     2461 gtgcgtaaag caaagggagg caggcaaaag ggggaggtag ttggtgttga tgatcagtgt
     2521 aaggagcata aggaatgaaa aaaatttttc attcttgaat ataaaaaaca gatgccctta
     2581 ttctggtatt aatacaaggc tgttttactt gaacttataa taactgcaac tgttacatcg
     2641 tatctggaaa acgcctcgca aaaataaaaa atgatgcgta aatgagcttt ctttaaagat
     2701 tcggcaaggg tacttttctc tccatagatt caaaaatgat attggcgaga tatttatgaa
     2761 ctctctggca ggtatagata tgggcagaat tttactcgat ttatcgaatg aggtgattaa
     2821 gcaacttgat gatctcgagg tgcagcgtaa tcttcctcgt gcggacctat taagggaagc
     2881 ggtagatcaa tacctgataa atcaatcgca aacagcaaga accagtgttc ctggcatctg
     2941 gcaagggtgt gaggaagatg gtgtcgaata tcagcgtaag ctgcgcgagg aatggtaatg
     3001 ggaaaaaatg atgtcaatca gattgctgac aacgtgcgcg ttgttcatgc cggatgcggc
     3061 gtgaacgcct tatccggcct acaaagtcat gcaaattcaa tatattgaag tttattggta
     3121 ggcctgataa gcgcagcgca tcaggcaatc atacgcttgt catcagtgac atacccataa
     3181 ccttatcgat gtgttggagg gaagaacgag gcaaacagac aattgcggca tgatcttgaa
     3241 tgggtaacgc ggaatacgag agatggtgtc gtcacaccct atgagctgta atccgggcga
     3301 cctgcgccac ccggaaaacg tcagagatta ctctccttca cccggaaaca ggaacgggtt
     3361 aatggaacta cgggcgtagc cttcttgctc cattcgcgcg tccagtacca gagaggcgag
     3421 gtcatctgcc acggcttcaa ctttcggttc tttttcctga taaagaatct tcaggtaagt
     3481 gccgcagtca tcgcagcttt cggctttaat cgcggcctgt tcgtcatcca gcgaccagta
     3541 atgcagtttg ccgctctgct cgcagttgct gcattttacg cgcactacgt gccattcggt
     3601 ttcacacagg ttgcagtgca ggtaacgcag accctgagtg gtgccaattt gcaccatgct
     3661 ggacaccggc atagagccac ataccgggca atattgacgt tgttcgccgt attcagcgcg
     3721 ggctttgccg gggatcagat tggccatctg cgcccagtag agcgacagcg cagcccagat
     3781 aaacggcgct ttatcgctgc tgacggacga gaaatcagag gcaaacagcg cgctggccat
     3841 atcttccagc tcctgagtcg atgccttctc cagattctca atcactgcca gcgccgggcc
     3901 gctcatttca ggtttcagct cagcaattag cgccatcagc agcttttgcc agtgcttatc
     3961 acgcggcaga acgtgaatat ccagcggggg cttgccctgt gcgctggctt ctttaatgcg
     4021 cgcggtcaga tccatctcca gcggatggtc gtacagcacc actttctggg cgtgggcgat
     4081 aagcgcagca aagcgcaggt aatcacccag cggattattt tctgccagct cgcgcagacg
     4141 ctcggcgcgg cggttgtata aattcttgag ccgagggaac aataacggcg gaatcatatc
     4201 cgccgtacgt ttctcgctcg aacccagctc atcttgcggg attatgcgaa tactcattca
     4261 gctttctttt ccgttgtctt gcggacctca cggtaccagc gcgggtgatg tttcttcgcc
     4321 caggcgctgg taacccatcc ttccaccatg gcggtaatcg tgcctttcac ccaaagggcg
     4381 gcgtagatat gcaccatgat aaccacaatt aacgccactg cggcaaatga atgcagcatt
     4441 aacgcgaatc ggatcaccgg gattgagaaa gcaggcgcaa aataaggacg ccagataatc
     4501 acgccgctca ccagcagcag aaccaggaaa ataatcgccg cccagaaaac gcatttctga
     4561 ccgaagttat aacgcccggt gtcacctact tcctcgttga cgacgatctt acgaatattc
     4621 ttcgcccaaa agatatcatc ccgattgatt aggttttgat gccagtaacg gaaaaacatg
     4681 atgatgaacg aggcaaacat aaccacgccg acaaacgggt gcagaattcg cgccagctgc
     4741 ggtgtgccca tgatttgcat caaccagttg aaggacggga acaaaaagcc cagcccgctc
     4801 accgccgcta ggatgaagca gaaggcggtg atccagtggt tgatacgttc cggcgcggtg
     4861 tagcgcacga tggtgtcacg tcgtttcatt tgcgctcctc gtctttctct tcgtgcagat
     4921 tattctcttc ctcatccgca cggttcggac cgacacccac gtagtggaag atactggccg
     4981 caaaggtagc cgcaaagcca acagccgcga gcggtttcca gatgcctttc cagaatttca
     5041 cggtttcgct gatttccggg ttctccggca agccatgata cagatttggc ttgtcagcat
     5101 ggtgcagcac gtacatgacg tgtgtaccac cgacgcctgc cggatcgtac agacccgcat
     5161 tgtcgtaacc gcgggttttc agctcagcaa cgcgctcgct cgccagcgtt ttcatcgact
     5221 ctttcgtgcc gaagtgaatc gcgcccgttg ggcaggtctt cacgcaggct ggttcttgcc
     5281 caaccaccac gcggtcaacg cacagcgtac atttgtagac gcggttgtct tccgggttga
     5341 ggcgcggaat gtcgaacgga cagcccgcaa tgcaataacc gcagccaatg cactgctcgg
     5401 actggaagtc gacgataccg ttggcatact gaataattgc accttccgcc gggcacgctt
     5461 tcaggcagcc tggatcggaa cagtgcatac agccgtcttt gcggatcagc cattccagtt
     5521 tgtcgttctg ctccacttcc gagaagcgca ttaccgtcca cgatttggcg cttaaatcat
     5581 tggggttgtc gtacacccca atgttattgc cgacggtatc gcgaatgtcg ttccactctg
     5641 aacaagccac ctgacaggct ttacagccga tacaggtggt aacgtcgatg agtttcgcca
     5701 cttcttcctg gaagtcccgc gcctgaggcg cgggggtcag accgttagtc gcggaacgac
     5761 gaatgatatc ttgagattga taagccatat gtcgtctccg ttacaccttt tccacattca
     5821 caaggaagga cttaaactcc ggcgtctgcg tgttcgcatc accaacgaat ggcgttaaag
     5881 tattggcaat aaagcctttt ttagctacgc cttcatagcc ccagtgaatc ggaataccga
     5941 tggtatcgat atctttgccg ttcgctttca gcgtgcgaat acgtttggtc accaccgctt
     6001 tggctttgat atagccacgg ttggaggaga ctttcacggt atcgccctgg gcaatgccaa
     6061 gtttattcgc cagcgactcc ccgatttcca caaactgctc tggctgcaaa atcgcgttca
     6121 acagcgcgtg tttggtccag tagtggaagt gctcggtcag acgataggtg gttccgacat
     6181 acgggaactt atcggcttta cccaatgctt cggcgtcgtc tttaaagata cgtgcagctg
     6241 ggttcgagat aacgtttgga tgcagcgggt tagttcccag cggcgtttca aacggctcgt
     6301 agtgttccgg gaacggacct tccgccatct tatcgagggc aaacagacgc cccatgcctt
     6361 cctgctgcat gataaacggc ccgacgccgc tacccggagg cgctgcgctg tagtccggaa
     6421 tatcccagcc ggtccactta gtgccgtccc atttcagcaa ctgacgcttc ggatcccacg
     6481 ggttaccctg cggatctgcg gaggcgcggt tatacagaat gcggcggtta agcggccatg
     6541 cccatgccca gcccagcgtg ttaccgaggc cagacggatc ggcgttatca cgacgcgcca
     6601 tctggttgcc ttccggcgtc cagctaccgg cgaaaatcca gcagccacag gaggttgtac
     6661 cgtcatcgcg caattgggcg aacgagctaa gttgttggcc tttcttgacg ataaccgcac
     6721 cggttgccgg atcggtaata tcggccagcg ccttaccgtt gctctccatc gctacttctt
     6781 ccgacttcgg ctcatgcgga atggcgtagt tccaggtcat gttcagcacc tgatccgggt
     6841 tcgcgccacc ctgttcggca tacatcttgc gcaagcgcag gaagataccg gagaggatct
     6901 caccgtcagt cagcgcaatc cccggggcgt ccgcaccttt ccagtgccac tgcaaccagc
     6961 gacctgagtt aacgattgaa ccgttctctt ctgcgaagca ggtcgatggc agacggaaca
     7021 cttcggtctg gatcttcgac gagtcaactt cgttcagctc accgtggttc tgccagaagt
     7081 tagaggtttc agtgttcagt gggtcgatgg tgacgaggaa cttcagtttc gacagacaac
     7141 cgatcacttt gtttttgttc gggaatgagg caacagggtt aaagccctgg cagatatagc
     7201 cattgacctt gccctctttc atcatctcga agtattgcag gacgtcgtag cctttatccc
     7261 acttcggcaa ccagtcaaag ccccagctat tttccgccgt cgctttatca ccaaagaagg
     7321 ccttcatcat agagacgaag aatttcgggt agttgcccca gtagtttacc tggccttcca
     7381 gcagcggttt tggcgtgttg gcggtaaggt aggtttgcag atcggtctgc ttctcgcttg
     7441 gcagcgtcat gtaacctggc agactctgcg acagtagccc caggtccgtc agcccctgaa
     7501 tattggagtg accgcgcagg gcgttaacgc cgccgccggc catccccata ttgccgagca
     7561 gcagctggat catcgccatc gtacgaatgt tttgcgcacc aacggagtgt tgcgtccagc
     7621 cgagggcata caggaacgag gcagttttat cgtgagcact ggtttctgcg atgtattcgc
     7681 agactttcag gaacgcgtct tttggcgtac cacagatgtt ttcaaccaca tctggcgtgt
     7741 agcgggaaac gtgctgtttc agcaagttcc acacgcagcg cggatgttgc agcgtggtat
     7801 cgcgtttggc gaagccgttt tcgtccagtt cataagtcca ggaggattta tcgtacttgc
     7861 gtttttccgc gtcgtagccg gtgaacaggc catcttcaaa gccgtaatcc tcacgcacga
     7921 tcaggctggc gttggtatag gcttcggtgt attcgtggtt gaatttttca ttgttcagca
     7981 ggtacagcaa tacgcctgac aggaaagcaa tgtcagtacc ggaacgaata ggggcatagt
     8041 agtcagctac cgccgccgta cgcgtaaagc gaggatcgat cacaatcagc ttcgcgccgt
     8101 tgtgaatttt ggcttccatc gcccagcgga acccgaccgg gtgagcttca gcggcgttac
     8161 cgcccatcac cacgacgagg ttggcgttct tgatgtcgac ccagtggttg gtcatcgcac
     8221 cgcgaccaaa tgttggagca agacttgcta ccgttggtcc gtgtcagaca cgcgcctggt
     8281 tgtcgaccgc gagcataccc agcgcgcggg agaatttttg cgttaaatag ccggtttcgt
     8341 tactcgacgc ggaagcacac agcatcccgg tggagagcca gcggttaaca gtcacgcctt
     8401 cggcgttttg cgcaatgtag ttagcatcgc ggtcttcttt catcagtttg gcgatgcgat
     8461 caaacgcctc ttcccaactg atttgctgcc atttatcaga acctggcgca cggtattccg
     8521 gaaacttcag acggctttcg gagtggatga aatccaccag gccagcgcct ttcggacaaa
     8581 gtgcaccgcg gttgaccggg tgatccggat cgccttcgat atggaagata gatgctttgg
     8641 cgttttttgc tccgtcaccg aggctgtaca tcaacagccc acagcctacg gaacaatagg
     8701 tgcaggtatt acgggtttcg cgggtgcgca gcagtttata ctgccgggtt tccgcgagtg
     8761 ctacgctggg tgcaaaaccc agtgccgctg ccgtggtgcc tgccataccg ccagcgcaga
     8821 tcttaaagaa ctgccttctg ctgacctgca tggattgctc cttgtttcga cattgtcacg
     8881 tcccatttac attcgcttgc tgcgtgtgca gggagtggga gttatttttc tttgcggaag
     8941 gggccgcaaa ggtccagaat tggctcaatt tccctccatc taggaagggt ttgtaacaga
     9001 ataccataat gttggtgtgt atgttcttat ctggttaaga gaaagtgaaa aaaacacagc
     9061 gaaaagaaat cgaaaatgtg acaaatatca caggtgttcg tcaaattgag ttatggcggc
     9121 gtgatgattt acaacaccca cggttagatg aggtcgcgga agaagttccc gttgcgctgg
     9181 tctacaacgg catttcgcat gtggtgatga tggcgtcgcc caaagacctt gagtactttg
     9241 cgctcggttt ttcgctttcc gaggggataa tagaaagtcc gcgcgatatc ttcggcatgg
     9301 atgtcgttcc ttcctgtaat ggtcttgaag tacaaattga gctttccagc cgccgcttta
     9361 tggggttgaa ggagcgccgc cgggcgctgg cgggacgtac gggctgtggc gtatgcggcg
     9421 tggagcaact taatgacatc ggaaaaccgg tgcagccgct accgttcacc cagacgtttg
     9481 atctcaacaa actggatgat gcattacgtc atctcaacga ttttcagcca gtggggcgac
     9541 tgactggttg tactcacgcc gctgcctgga tgttgccatc tggcgaactg gtcggcgggc
     9601 atgaagacgt gggtcgccat gtggcgctgg acaaactgtt aggccgccgg tcacaagaag
     9661 gggaaagctg gcagcaaggt gcggtactgg tttccagtcg tgccagttat gaaatggtgc
     9721 aaaagtcggc gatgtgcggc gtagagattt tgtttgcggt gtctgccgcg accacgcttg
     9781 ctgtagaagt ggctgagcgc tgtaatctga cactggtagg tttttgtaaa ccgggtcggg
     9841 caacggttta tacccatccg cagcgtttga gcaattaatt aaaataagtc ctgcgatata
     9901 tattaaattg caggacttat tcatttc
//
LOCUS       DAEEGA010000118         9905 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEEGA010000118 DAEEGA010000000
VERSION     DAEEGA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.118"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(160..900)
                     /gene="pflA"
                     /locus_tag="KLH46_003532"
     CDS             complement(160..900)
                     /gene="pflA"
                     /locus_tag="KLH46_003532"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBE0380083.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="KLH46_003533"
     CDS             complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="KLH46_003533"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBE0380084.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3429..4286)
                     /gene="focA"
                     /locus_tag="KLH46_003534"
     CDS             complement(3429..4286)
                     /gene="focA"
                     /locus_tag="KLH46_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBE0380085.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4692..6452)
                     /locus_tag="KLH46_003535"
     CDS             complement(4692..6452)
                     /locus_tag="KLH46_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HBE0380086.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6582..7208
                     /locus_tag="KLH46_003536"
                     /pseudo
     CDS             6582..7208
                     /locus_tag="KLH46_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7407..8495
                     /gene="serC"
                     /locus_tag="KLH46_003537"
     CDS             7407..8495
                     /gene="serC"
                     /locus_tag="KLH46_003537"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBE0380087.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8566..9849
                     /gene="aroA"
                     /locus_tag="KLH46_003538"
     CDS             8566..9849
                     /gene="aroA"
                     /locus_tag="KLH46_003538"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBE0380088.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2520 a         2368 c         2482 g         2535 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag
      541 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg
      601 aatgccttct tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc
      661 ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
      721 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc
      781 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
      841 acctgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
      901 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
      961 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
     1021 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
     1081 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
     1141 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
     1201 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
     1261 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
     1321 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
     1381 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
     1441 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
     1501 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
     1561 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
     1621 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc
     1681 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta
     1741 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
     1801 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
     1861 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
     1921 tccatgaagt gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc
     1981 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
     2041 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
     2101 atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc
     2161 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
     2221 ttgaagttca gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc
     2281 cccatagtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca
     2341 tcgaggccca taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca
     2401 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
     2461 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
     2521 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
     2581 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
     2641 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
     2701 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
     2761 tgtagagagg tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca
     2821 acgcgacggt agtcaccgat gatacggcca cggccataag catctggcag accggtcaga
     2881 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
     2941 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
     3001 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
     3061 ggagcttcag tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg
     3121 tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt
     3181 tccagtttaa caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
     3241 gccaggaagg actcgtcacc ctcgtacggc gtgtagtttt tctgaatgaa gtcacggacg
     3301 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
     3361 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
     3421 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
     3481 aacaaaccac cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag
     3541 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
     3601 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
     3661 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
     3721 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gtttgccagg
     3781 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg
     3841 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
     3901 acaaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac
     3961 tgacaccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag
     4021 agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc
     4081 agttttgcca tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg
     4141 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
     4201 ttcgttgctt tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa
     4261 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
     4321 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
     4381 ccgtaactcg catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa
     4441 taaattttaa aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta
     4501 attaaaaggg agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa
     4561 ttaccgatat ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg
     4621 ggcattaaaa aaacgccccg taatataact cagactaatc attaagccta cattgcgtag
     4681 gctatttgat tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc
     4741 ttcaacaacg actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg
     4801 taaaacgccg cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac
     4861 atgcgaacaa acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat
     4921 aacaacgttt gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta
     4981 aactccatcg tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc
     5041 agcatggctt ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc
     5101 agacccaaca gctcacgcac gcgggtaaag tcatcaaaat cttcttcatc cagttgctcg
     5161 atgaggttca ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca
     5221 cgtaaatggc tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg
     5281 gacatgccag gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata
     5341 taaacttctt tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg
     5401 gtgccggaga aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag
     5461 gagattaaac cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct
     5521 tcatcatcga aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc
     5581 agctcggtca cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca
     5641 aagcaggtgc cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca
     5701 agggaaccat catatgcgaa gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca
     5761 accaccgctg ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca
     5821 gcaataatgc ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct
     5881 tcgttgcggg tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc
     5941 atcggaatat aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca
     6001 cgatcttcgt taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga
     6061 tcgtaaaatg cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcgctc
     6121 agtgggaacc atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc
     6181 agccagaaat cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca
     6241 gaagccagcg ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct
     6301 ttgtcgcgaa tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca
     6361 atctgaaagc cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg
     6421 gcatctttgc cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg
     6481 cgcaataata cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt
     6541 gactatgctt ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc
     6601 accgtatggc atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc
     6661 tttatacctt tgtactggtt ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca
     6721 gatgtcgctg tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc
     6781 gttttatgat gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt
     6841 ataccgcctg gtaatatgat ggcgagctgg cctggtcgaa actcaataac tccaacatga
     6901 cggaatttga gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac
     6961 gtctggcgat tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg
     7021 tgaaaccggg tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgccgg
     7081 agtcggcgga ctatgcctgc attcgttgta gtgaaatcat tcatatgaac gcgggggaaa
     7141 aacaattatg tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg
     7201 tgacctgaca gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat
     7261 gcaagccaca tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata
     7321 aaaccgatag ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta
     7381 atagcaacgc aacgtggtga ggggaaatgg ctcaaatctt caattttagt tctggtccgg
     7441 caatgctacc ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc
     7501 ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg
     7561 aagccgagaa ggattttcgc gatcttctta atgtcccctc caactacaag gtattattct
     7621 gccatggcgg tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa
     7681 ccaccgcaga ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa
     7741 aatactgcac gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta
     7801 agccaatgcg tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg
     7861 aaaccatcga cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg
     7921 ccgccgactt ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa
     7981 tttacgctgg cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg
     8041 aagatttgct gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca
     8101 acgataacgg ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg
     8161 tctttaaatg gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa
     8221 aagcagaact gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga
     8281 aagctaaccg ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat
     8341 tgttccttga agagtctttt gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg
     8401 gcggaatgcg cgcgtctatt tataatgcca tgccgctgga aggcgttaaa gcgctgacag
     8461 acttcatggt tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac
     8521 agccagcctg tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg
     8581 ttacaaccca tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct
     8641 aaccgcgctt tattgcttgc ggcattagca cacggcaaaa cagtattaac caatctgctg
     8701 gatagcgacg acgtgcgcca tatgctgaat gcattaacag cgttagggtt aagctatacg
     8761 ctttcagccg atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa
     8821 ggtgccctgg agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct
     8881 ctttgtctgg atagcaatga tattgtgctg accggtgagc cgcgtatgaa agagcgcccg
     8941 attggtcatc tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa
     9001 gaaaattatc cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgat
     9061 ggctccgttt ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa
     9121 gatacggtga ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc
     9181 aatctgatga agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta
     9241 aaaggcgggc agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg
     9301 gcttcttact tcctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt
     9361 ggacgtaaca gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg
     9421 accatttgct ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat
     9481 atggatatga accatattcc tgatgcggcg atgaccattg ccacggcggc gttatttgca
     9541 aaaggcacca ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg
     9601 tttgcgatgg caacagaact gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac
     9661 attcgtatca ctcctccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac
     9721 cggatggcga tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat
     9781 cccaaatgca cggccaaaac atttccggat tatttcgagc agctggcgcg gattagccag
     9841 gcagcctgaa tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgtcggca
     9901 cattg
//
LOCUS       DAEEGA010000119         9783 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEEGA010000119 DAEEGA010000000
VERSION     DAEEGA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.119"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..143
                     /locus_tag="KLH46_003539"
     CDS             <1..143
                     /locus_tag="KLH46_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0380089.1"
                     /translation="AEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            complement(194..514)
                     /gene="psiF"
                     /locus_tag="KLH46_003540"
     CDS             complement(194..514)
                     /gene="psiF"
                     /locus_tag="KLH46_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBE0380090.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(633..2048)
                     /gene="phoA"
                     /locus_tag="KLH46_003541"
     CDS             complement(633..2048)
                     /gene="phoA"
                     /locus_tag="KLH46_003541"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBE0380091.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2149..2409)
                     /gene="iraP"
                     /locus_tag="KLH46_003542"
     CDS             complement(2149..2409)
                     /gene="iraP"
                     /locus_tag="KLH46_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBE0380092.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2591..2794)
                     /locus_tag="KLH46_003543"
     CDS             complement(2591..2794)
                     /locus_tag="KLH46_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380093.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2872..3966
                     /gene="ddlA"
                     /locus_tag="KLH46_003544"
     CDS             2872..3966
                     /gene="ddlA"
                     /locus_tag="KLH46_003544"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBE0380094.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3990..4202)
                     /locus_tag="KLH46_003545"
     CDS             complement(3990..4202)
                     /locus_tag="KLH46_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBE0380095.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4462..4770
                     /locus_tag="KLH46_003546"
     CDS             4462..4770
                     /locus_tag="KLH46_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBE0380096.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4829..5923)
                     /gene="yaiW"
                     /locus_tag="KLH46_003547"
     CDS             complement(4829..5923)
                     /gene="yaiW"
                     /locus_tag="KLH46_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBE0380097.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPRKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5936..7156)
                     /gene="sbmA"
                     /locus_tag="KLH46_003548"
     CDS             complement(5936..7156)
                     /gene="sbmA"
                     /locus_tag="KLH46_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBE0380098.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVAHITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7508..8665
                     /gene="ampH"
                     /locus_tag="KLH46_003549"
     CDS             7508..8665
                     /gene="ampH"
                     /locus_tag="KLH46_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBE0380099.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8666..9169)
                     /locus_tag="KLH46_003550"
     CDS             complement(8666..9169)
                     /locus_tag="KLH46_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0380100.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9257..>9783)
                     /locus_tag="KLH46_003551"
     CDS             complement(9257..>9783)
                     /locus_tag="KLH46_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE0380101.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2437 a         2493 c         2333 g         2520 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 tggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcaatcag gtatcaggcg gctttcttga ggcaggcact cataaactta ttacgatcat
      241 cacctttcag agcttgttgc gttgcttgat tattacattc acgcatcttt tgctgctgtg
      301 gcgtcaaact tttttcacca ggcgcagact tgctgttctt caggcaatca ctcatgtagg
      361 tcttacgagc atcccctttc aacgcctgcg ccgtcgcctg ctgattacag gaggtcatac
      421 gctgttgttg tggggttaag gttctctcgg cagcgccgac ggtggttaaa aaaaccagac
      481 caaaaagcaa ggtaaccagt aatgttattt tcatagcacc atccctcttc atgttttaac
      541 catgagcgtt tgcgcccgtg atctgccatt aagtctggtt gctaacagca aaaaaaccac
      601 ccggcagcga aaattcactg ccgggcgcag ttttatttca gccccagagc ggctttcatg
      661 gtgtagaaga gatcggtctg gtcggtcagt ccaacaacat tggcggcatg cgggccatac
      721 gccgcaatac gcaactgact gccggtatgt tcttgtgaat cctcttcgga attcccgtaa
      781 ctcatcacca tcactgcgcc atctttagta tttagcgcct gagtgaggcc cggcgctttg
      841 gtatccggcg caacaacctg gctggcgtgg gcgtgatcag cggtgactat gaccagcgtg
      901 ttaccctcct ttttagcgaa ttccagcgcc cgttgtacgg cttcatcgag atcgaccgtc
      961 tcgccaattt gcccacaagg gttcgcagcg tgatcctgtt tatcgattga cgcaccttca
     1021 acttgcagga aaaatccttt ctcattttta ctcaacaatt caatggcttt gtcggtcatc
     1081 tgtgccaggg ttggtacact gtcattacgt tgcggattag gcgtataggt gactgcgggc
     1141 ttatcgatat tgccgtggta cgttgctttc ggccctagcc agcgcactgg catattgccg
     1201 tcagcaaaca ggccaagcag gggtttttgc tgattcgctt ccgtcaccga attcagtgag
     1261 gcagcatcgt tcaccaactg ataaccacgc gcctgtgcct gttcacgcag cgtttttccc
     1321 tgccattcac cagcggttgc cgtttcagca aaggtttttg cgccgccgcc aagcgtaacg
     1381 tcggcacgag cgttaagcag ctgttcggta atcgatcctt ttccgccttt ttccagagcg
     1441 ttacccggac atttttcact ggtcgcgctc ggaccgtagc atttgcgcga agtcacatgt
     1501 gccaccagcg cagcgggcgt ggcatcctgc aactctgcgg tagaaacgtt accggtcgcc
     1561 agacctgcgg cttttgccat ttccagaatc gttgggtgat ctttttcgtg aatatcgacg
     1621 cccagcgcgc cgttataggt tttgacaccg gttgaccagg cggttgctga tgcagccgag
     1681 tcggtgacgt agtccggttt gccagttttt ttattcagcg catagtgagt gtattgcccg
     1741 gtaagcggta aggcatcgat acctttaaaa aagccgcccg caccttcggc ataattacgt
     1801 gcggcagtaa tttccgaatc ccccatccca tcgccaatca gcaaaataat attttttgca
     1861 ggtttatcgc taagagaatc acgcagagcg gcggtctgat cacccgttaa gcggcgagca
     1921 ccgccgggtg cagtaatatc gccctgagca gcccggtttt ccagaacagg catttctggt
     1981 gtccgggctt ttgtcacagg ggtaaacagt aacggtaaga gtgccagtgc aatagtgctt
     2041 tgtttcactt tattttctcc atgtacaaat acattaaaaa ataaaaacaa agcgactata
     2101 agtctcggcc gtgacaactt tatgacagct gttgaaaaga ttaacttttt actgacaagg
     2161 atgcttcaat aacttcttta cgtaatcgcg cagcagctct gtatcgtcgt caggaatgct
     2221 ggcatcgggc tttacctcgt acagcgcccc ctctacctga tcaatcaacc gctgttggtc
     2281 attttgcgcc atattgcgaa gcattgcagt gacgataatc tccaaagctt ctacctgcgc
     2341 acacagttct ttcgactctt cttctttttg ggcaagctta aataacaact cagcaatgag
     2401 atttttcatg tctgtatttc cttatccaaa gtatggagaa gttaacatta tgtttcacaa
     2461 cttcatagtg tctggaagta ttatttttat gacaaaggaa atactttgcg caaatatttt
     2521 ttgtttgatt accagcgaaa cgtttctctt atttaaataa ttacccacac aaaatatagg
     2581 cttttaatta tcaaccttta ttataaataa aagctatgaa atatattctc ttcgttaact
     2641 tataattaag agaaaaaaac tcaccgcatt gccatttaag ccaggaataa attagcttcc
     2701 cgttcatcat ttttgtgatc ttgcgcacat ctttgagata acgcactttc ttcaccagta
     2761 cgttgcacgc tttgtcagca gaagattgca acaatctttc agaaggccgc gaatcccccg
     2821 ttttgtcgtg gtaatgtatg cactttgtgt tagacgggat aggtttttaa gatggaaaaa
     2881 ctgcgggtag gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc tctgcaatcg
     2941 gcaaaaaaca ttgtcgatgc cattgataaa agtcgcttcg acgttgtgct gctgggcatt
     3001 gataaacaag ggcaatggca cgtcagcgat gccagcaatt atctgctaaa tgcagacgat
     3061 cctgcccata ttgcgttgcg cccttcggcg accagccttg cgcaggtgcc aggtaaacat
     3121 gagcatcagc ttatcgacgc gcaaaacggt cagccgttgc cgacggtgga tgtcattttc
     3181 ccgattgtcc acggtacgct gggcgaagat ggttccttgc agggaatgct gcgggtcgcc
     3241 aatttaccgt ttgtaggttc tgatgttctg gcttcagcag cctgtatgga taaagatgtc
     3301 accaaacgtc tgctacgcga tgccgggctg aacattgcgc catttattac cctgacgcgc
     3361 gctaatcgtc acaacatcag ttttgccgaa gtggagtcta aactggggtt accactgttt
     3421 gtaaaaccgg ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag tgaagaacag
     3481 tacgcaattg ccgtcgatct ggcgttcgag ttcgatcata aagtgatcgt tgagcaaggg
     3541 atcaaaggtc gtgagatcga atgcgcagtt ctgggcaacg acaatccgca agccagcacc
     3601 tgtggcgaga tcgtactcac cagcgatttc tatgcctacg acaccaagta cattgacgaa
     3661 gatggcgcga aagtggtagt tccggcagcc attgcaccag agatcaacga taagatccgg
     3721 gcgattgccg ttcaggctta tcagacgttg ggatgcgcag gcatggcgcg tgtagacgtg
     3781 tttttaaccc cagagaacga agtggtgatc aacgagatca acacactgcc tggcttcacc
     3841 aacatcagta tgtatccgaa gctgtggcaa gccagcggtc tgggttacac cgatctgatc
     3901 acacgtttga ttgagctggc gctggagcgt cacgctgcgg ataacgcatt gaaaaccaca
     3961 atgtaatatt ttaacgcccg ataatattct tattgggcgt tttcagtttc ttcatcgcga
     4021 cgacgaataa tcaaccccgc cagccagaaa ctaatcaccc acgtcaccag accgacggca
     4081 taggtctgcc aaccttttgc ttcaaatccc agtaccccca ccacgccatt aagaatgaat
     4141 ataaggccaa tggcgaacgc atagtagtgc cagtcacggc ggatttttac aggcagattc
     4201 atggcagctc cgttaacgaa aaacaacatc cttgcatatt ctgtaacaac cgtctgtggt
     4261 gtagacaaca ttttcgtaat tattaataaa tttaacttaa ggttgccatt ttgtgactca
     4321 caacgaagat tcacgcttcg ggaatattcc caacatcaat ttaccatgat tctgatattg
     4381 acaatctaaa tgacctgtca gacttgccat aagccgctgg tatcacacca gaagagaagc
     4441 agatgacttc cggaggtttt tatggctgat ttcaccctgt caaaatcgct gtttagcgga
     4501 aaatatcgca atgcctcttc aacgcctggc aacattgcct atgcgttgtt tgtgctgttt
     4561 tgcttttggg ctggggcgca attgctgaac ctgttagtgc atgcgcccgg cgtctatgag
     4621 cgtttaatgc aggtccagga aacaggtcgc ccacgggtgg aaattggttt aggtgtcggc
     4681 accattttcg ggctgatccc gtttttagca ggctgcctca tttttgcagt ggtggcgcta
     4741 tggctgcact ggcgacatcg ccgccagtaa aatttacgtt gccagatgcg gcctgaaacg
     4801 ccttatctgg cctgacattc ctgtttgatc actgtttcat acaacgcgcc cgccgttcgt
     4861 ctacgcgctt cgcaaaccag gccgtagtca ggttgcgggt gattttcggg ctttccagtt
     4921 gaatgccagg caacatttcg cggggtagtg atttacccgt tttcgcttcg gcaagttgat
     4981 aaactttctt gtacagcgcc gtctcctcaa aagagaagct gtcacctttc tctaactgac
     5041 ggcgaatttc gctgtcgctc atccccagtt ttcccgccag tttgcgcgtt gccagttcgg
     5101 ttttcctggg ttctttactg tcgtaacgaa ttaaatcacc gtccagtgcc agcttcacgc
     5161 cgctggcctt actgactgcg ttctgaaacg cggcattacg gctggcgtac cagccagcgt
     5221 taaaatcagc aaagcggtat atcggtgcgc tatagctggc gggataattc agtaaatggt
     5281 aagtaccaaa ccacaacccg ccgcggcgac tgaagacttc ctgacgaact gtaccgtcca
     5341 ttttccacgg ataccctttg gtatgctgtt cggcaaaagc aatgctgacc tgcatcggcc
     5401 caccggtgcg caccggatta agcgaaccaa acagcgtctg ccccattggt accatgctga
     5461 tcagatcgtc aaaaatcgcg ctcaattgct tttctgtacg caccgaatct aaccgttcgc
     5521 tataactttt gccgtttggc gatttgattt ttagcgcggt atgaaccaga aaagcaggaa
     5581 tgtgcatccg ttcagcacga cggtcaatct cttgccaggc gatcttgctt aagcccggaa
     5641 cggccggatc tgcctgataa tttgactcct gctgcgccac cgccagcacc gagtagacgt
     5701 tttccagcgt cggtgccagc ccctgacttt caaaggtggt ggcgagatct ttcgcccagg
     5761 catcgcggtc tttcacgctc gcaggcatct tctgccgcac gacgctcgct acatctatcg
     5821 ctttttcgcc ctttttcaac ggttgcggag cctgactact acaccccgct aaaaccagaa
     5881 ccgccaacaa tgacagcgaa gatagatgat tgacgcgcga cattacttct cctttttagc
     5941 tcaaggtatg ggttacttcc tgaattttgt caccatccag ctcatgttca aagctgcgca
     6001 gacgtttgta gatagacatc aactcaacca gtgtggtcca tgagttaatc aggtactgga
     6061 aagcaccgcg aacctgacca aaaacgttgg taatctgcgt catcaggccg agcgtaatcg
     6121 taccggcaac aattgacgga aacagcaaga acaaaccgaa aacgttatcg acctgcaaat
     6181 agagaatgcg ggcgatgttg aaatacatat agtgaaaata gaggcgaaaa tagtttttcc
     6241 gtacggcgct aaacagctcg cgtaccgtag gcggcgtcgc gcgcgtggca tcgtcttcac
     6301 cataaaccag ctctttacgg taggcggcct ctactcgctg gtttttaaac tccagccccg
     6361 gcagtttgat ccctaccact gccagcaatc cggtccccat cagcgaccag acgatagcgg
     6421 caatcaccag accatacgga atgtgcccga taatcggcag ctccggcaca tgcgcggaga
     6481 gcgttaccag caccggcagg aaggcgatca acgtcattat ggcgttgata aaactgacgc
     6541 ccatattctc cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc tgtgcggcac
     6601 cttcgatatg acgcagttgt tgccagttcg ccatgtaata ttcgttcatc gccgtacgcc
     6661 agcggaacac gtagtgactg acaaagaagt tgttcagtac actgatcacc actgcgatca
     6721 gcgcaatccc cagaaagacg cccacttcgc ggtaaaattg ttcgatagtg actttatgcg
     6781 gcgaacttag cgcggtttga atcagatcat agaacggcgc ataccaggcg ttgacggcga
     6841 ccccgacttc caccaaaaac caagtgacga agataatcag cgcagtaccg agtattgacc
     6901 agtattgcca acggtgcggg ctgtagataa accagaaaaa tgcaaaaagt cctacgcaaa
     6961 caatgtagta agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg gcgctaatcg
     7021 ggatctgccc ggaagcgccg gtgatatgtg ccacccagtc acccccaccg gcttgccaga
     7081 agataacggc aatcaatgcc caaacaaagg ccgagagaaa aaacgttccc ggctttggga
     7141 aaaaagactt aaacatatgc actcctgcta acttcttatc gttttaacaa ctgctgtgta
     7201 ttaaccgcat gaccgctaca aagcgtcagc caaatgaaac cgcccattgt gacccatagc
     7261 aaggcgctta gttcgccagc ctgaccataa gaattgtttc gacggatttc acaaaccaaa
     7321 gattgtttac ataagcggat taacacgcgc aggatcacaa atgcaggcat tggtgagcag
     7381 gaaaaagatc gtgtttacgc gccgttacgt ttttaacact attattgcac taattcgccc
     7441 tttgcaatcc atcaatgagt agtataaata cgctcagtta ccttcattca atctatggac
     7501 accaccgttg aaacgtagtc tgcttttttc tgccgtgctg tgtgcggcgt cattgacctc
     7561 cgtccatgcg gcgcagccga tcactgaacc ggagtttgcc tctgatattg tcgatcgtta
     7621 tgccgatcat attttttacg gcagcggtgc cacggggatg gcgctggttg ttatcgacgg
     7681 taaccagcgc gtctttcgta gttatggcga aacgcgacct ggtaataacg ttcgcccgca
     7741 gctggattcg gtcgtgcgta ttgcttccct caccaaacta atgaccagtg aaatgctggt
     7801 gaaattgctc gaccagggga ccgtaaagtt aaacgatccg ctaagtaaat atgctccgcc
     7861 aggcgcacgc gtgccgacct acaatgggac gcccattacc ctagtgaatc tggcaaccca
     7921 taccagcgcc ctgccccgtg aacagcccgg tggcgcggca catcgtccgg tgtttgtctg
     7981 gccaacgcgc gagcaacgct ggaaatacct ttctacggcg aagctgaaag ccgcgccagg
     8041 cagtcaggcg gcgtactcta accttgcgtt cgacctgctg gcagatgcac tggcgaatgc
     8101 gtccggcaag ccttataccc agttgtttga agagcagatt acccatccat tggggatgaa
     8161 agacaccacc tacaccccct caccggatca gtgccgccgt ttaatggttg ctgagcgcgg
     8221 tgccagtccg tgcaataaca cgctggcggc aattggcagc ggcggcgtct attccacgcc
     8281 tggcgatatg atgcgctgga tgcagcagta tttgtcatct gatttttatc agcgcagtaa
     8341 ccaggcagat cgcatgcaga cgttgattta tcagcgcgcg cagtttacga aagtgattgg
     8401 catggatgtc cccggcaaag cggatgcgct tggtttaggc tgggtatata tggccccgaa
     8461 agagggtcgt ccgaggatta ttcagaagac aggcggtggt ggcggtttca ttacctatat
     8521 ggcgatgatc ccgcagaaga atattggcgc gtttgtggtg gtcactcgtt cgccgttgac
     8581 gcgctttaaa aatatgagcg atggcatcaa tgatctggtg accgagctaa gcgggaataa
     8641 accgttggtt atccccgcat cctgattaat actctgaagg caaacggttg atagcgacca
     8701 gtttgccttt attcatttcg atatagccgc ctttacgcaa agcagcgaga acttctgcga
     8761 cgacagaacg cgatatgcgt gtacgttgat ggatataatt catcacacca atacgtgatc
     8821 gcaattcttc attccagtca atcattgata ataatgttgc gcgggtttgt ttggctggta
     8881 gatgatatcc cgtacaattt ccttctgata ttaatttgta tggtatatcg tttttcatta
     8941 aaccatcagc cagccccata atataaggag cctgggtaat accgataagt acgttttctt
     9001 ctctacgcag agagataact ccttctaaaa taacgaaagt atcttcgtta ttgacatcac
     9061 tggaaaatat aacttgcttt tcattattaa actcgaagcg cgtaccgtat ctggacaaac
     9121 atttatcgag cttaccaaat tcctgaagag gtttaactac agataacatt tgcgcgtcct
     9181 ttgcagtaat gcccgtcaaa tccttgacgg gcattattta gattaaatta ccagtatttc
     9241 ttcggagtga agaatattac caagtatatt taacacccac gttcgcggac cagtcttgat
     9301 ctacgtcacc accaccgagg tagttagcat cggtataggc gctgaagttc ttggtgaagc
     9361 tgaactgagt acccagacca acacgtaccg cagacccttc agtaccgtta tcgatggaat
     9421 caccgttcac atcgttatcg ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg
     9481 gagtcagagc ctgatcttcg ctgtaggtga aggtataacc tgcatctaca cccagttcat
     9541 aacgcatgct gtcgtaaggc tgaccgtcaa ctttcatgtc gttgctcagc tggtagtcat
     9601 caccagactg gaacagacca gaaatgctgc cgtaaggagt cacgtaacca gcatcaccca
     9661 gtttgaagtc gtaaccggtt ttcaaaccga agccccaagc gtcggagttg gtgctaccgt
     9721 caacgtaagt accgttgctc atggttgcag acaggtcgtt gttgaagtga gagtagctca
     9781 agc
//
LOCUS       DAEEGA010000120         9644 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEEGA010000120 DAEEGA010000000
VERSION     DAEEGA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.120"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="KLH46_003552"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="KLH46_003552"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBE0380102.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="KLH46_003553"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="KLH46_003553"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBE0380103.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="KLH46_003554"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="KLH46_003554"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBE0380104.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="KLH46_003555"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="KLH46_003555"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBE0380105.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="KLH46_003556"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="KLH46_003556"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBE0380106.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5060..6193
                     /locus_tag="KLH46_003557"
     CDS             5060..6193
                     /locus_tag="KLH46_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBE0380107.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="KLH46_003558"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="KLH46_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBE0380108.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="KLH46_003559"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="KLH46_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBE0380109.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="KLH46_003560"
     CDS             complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="KLH46_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBE0380110.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="KLH46_003561"
     CDS             complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="KLH46_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBE0380111.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7739..8305)
                     /gene="efp"
                     /locus_tag="KLH46_003562"
     CDS             complement(7739..8305)
                     /gene="efp"
                     /locus_tag="KLH46_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBE0380112.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8347..9375
                     /gene="epmB"
                     /locus_tag="KLH46_003563"
     CDS             8347..9375
                     /gene="epmB"
                     /locus_tag="KLH46_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBE0380113.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2451 a         2473 c         2459 g         2261 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccaacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag
     2221 gcacgttggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc cccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg
     6361 gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
     6481 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gaaatttggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg
     7261 cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc
     7321 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
     7381 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
     7441 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
     7501 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
     7561 attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc
     7621 aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
     7681 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
     7741 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
     7801 gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
     7861 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
     7921 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
     7981 ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag
     8041 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
     8101 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
     8161 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
     8221 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
     8281 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
     8341 cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa
     8401 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
     8461 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
     8521 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
     8581 tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac
     8641 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
     8701 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
     8761 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
     8821 gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct ggactggttg
     8881 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
     8941 attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg ttctacgctg
     9001 caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac gttccgtcag
     9061 gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt tctgttacgt
     9121 ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt cgatgccggc
     9181 gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca ttttatggtg
     9241 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
     9301 gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
     9361 ctacgccagc agtaactgat taaacaggga agttaaacat taaaattgcc tgatgcgcta
     9421 cgcttatcag gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg
     9481 gataaggcgt ttacgccgca tccggcaaga gcaacaaacg caagtacagc aacctgtcat
     9541 taaactgaga aatttaatct ttgcacgttg cataaccttt aattattcat atgcattgct
     9601 gttgttactt tttatatagc taacaccaat aattaacctt tctt
//
LOCUS       DAEEGA010000121         9625 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEEGA010000121 DAEEGA010000000
VERSION     DAEEGA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9625
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.121"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="KLH46_003564"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="KLH46_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBE0380114.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="KLH46_003565"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="KLH46_003565"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBE0380115.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="KLH46_003566"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="KLH46_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="KLH46_003567"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="KLH46_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBE0380116.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="KLH46_003568"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="KLH46_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBE0380117.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="KLH46_003569"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="KLH46_003569"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBE0380118.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="KLH46_003570"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="KLH46_003570"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBE0380119.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="KLH46_003571"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="KLH46_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="KLH46_003572"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="KLH46_003572"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBE0380120.1"
                     /translation="MIRKAFVMQVNPDAHEEYQSRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2352 a         2515 c         2489 g         2269 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataagacca ttgtttttat ttttaaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa actacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacgtca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagag tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaat
//
LOCUS       DAEEGA010000122         9600 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEEGA010000122 DAEEGA010000000
VERSION     DAEEGA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9600
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.122"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(71..415)
                     /locus_tag="KLH46_003573"
     CDS             complement(71..415)
                     /locus_tag="KLH46_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBE0380121.1"
                     /translation="MQYLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(415..1341)
                     /gene="glsB"
                     /locus_tag="KLH46_003574"
     CDS             complement(415..1341)
                     /gene="glsB"
                     /locus_tag="KLH46_003574"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBE0380122.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(1405..2340)
                     /locus_tag="KLH46_003575"
     CDS             complement(1405..2340)
                     /locus_tag="KLH46_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBE0380123.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            2420..3535
                     /gene="yneK"
                     /locus_tag="KLH46_003576"
     CDS             2420..3535
                     /gene="yneK"
                     /locus_tag="KLH46_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HBE0380124.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            3685..4875
                     /gene="ydeA"
                     /locus_tag="KLH46_003577"
     CDS             3685..4875
                     /gene="ydeA"
                     /locus_tag="KLH46_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBE0380125.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(4900..5565)
                     /gene="marC"
                     /locus_tag="KLH46_003578"
     CDS             complement(4900..5565)
                     /gene="marC"
                     /locus_tag="KLH46_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBE0380126.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMLLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            5777..6211
                     /gene="marR"
                     /locus_tag="KLH46_003579"
     CDS             5777..6211
                     /gene="marR"
                     /locus_tag="KLH46_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBE0380127.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            6231..6614
                     /gene="marA"
                     /locus_tag="KLH46_003580"
     CDS             6231..6614
                     /gene="marA"
                     /locus_tag="KLH46_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBE0380128.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            6646..6864
                     /gene="marB"
                     /locus_tag="KLH46_003581"
     CDS             6646..6864
                     /gene="marB"
                     /locus_tag="KLH46_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBE0380129.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(6895..7794)
                     /gene="eamA"
                     /locus_tag="KLH46_003582"
     CDS             complement(6895..7794)
                     /gene="eamA"
                     /locus_tag="KLH46_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBE0380130.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            8058..9176
                     /gene="ydeE"
                     /locus_tag="KLH46_003583"
     CDS             8058..9176
                     /gene="ydeE"
                     /locus_tag="KLH46_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HBE0380131.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            9303..9398
                     /gene="mgtS"
                     /locus_tag="KLH46_003584"
     CDS             9303..9398
                     /gene="mgtS"
                     /locus_tag="KLH46_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBE0380132.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         2489 a         2433 c         2187 g         2491 t
ORIGIN      
        1 agcggcggca aataaaggta ccctgggaat gctattttat cgttttccgc aattctcatg
       61 gctgattcat ctatacaacc agccagtgat agattaccgc cacgatataa cgttgttgtt
      121 cttcgcttaa cgaaacaccc tgcggaatgt tatgccactt agccagacaa ccgcgacagc
      181 aggtcgcggt ggcgtgctga gcgataaaca ccggatgccc gcgcatgggg gtttgcttac
      241 cgtcattagc cggtaatgcc ggagcaagac gtttagcgac aaaatccgcc gcatgttgtt
      301 cgatgactgg cgcgcctttc tccaggcaat actgacgctc tttcatgccc agacgaaagc
      361 gagagcgaaa ttttgaacgg gataaacgcg cgaagagtgg atcgagatac tgcattaata
      421 aaccgaacgc cctaactgtt tcgtcaattg ttcaaggacg gcgatacccg caagcgagtt
      481 acctgcatca tccagttccg gactccagac tgcgatggcc atttcatgcg gaacaatcgc
      541 cacaataccg ccaccaacgc cagatttcgc cggtagtccc acccgccagg caaactcccc
      601 cgcgttctgg tacataccac tggtcgccat cagcgcgtta atttgccgcg cctgcattgg
      661 cgtcaccaca ggttcatcaa tatgaatagc tttcccctga ttagccagaa agacaaacgt
      721 ccgggccagc tctacacagc tcattttcag agcgcagtaa tgaaagtagt tttgcagaac
      781 ggttgtcacg tcatgatgga aattgccaaa cgacttcatc agccaggcga tagccgcatt
      841 tcgcgcggaa tgttcaaatt cggaacgcgc taccaccgta tcgtatgaaa tatcagacac
      901 accgcttaag ccgcgcacga cttccagcat acgttgccgt ggtgcgctta gtcgcccttg
      961 caacatatcg cagaccacca gcgcaccggt attaatgaac ggattacgcg gaataccctg
     1021 ttccatttcc agttgcacta aagaattgaa cggtgatcca gacggatctt tgccgacgcg
     1081 ttgccagatt tcctcttcgg agtaatgacg catggcgaca acgagactca gcactttgga
     1141 aatagactga atggaaaaac gttcttgcgc gtctccggcc tgaaaaagct gtccgtcaac
     1201 ggtacagata gcaatcccca atcgggaacc gtctactgta gccagcgccg gaatataatc
     1261 cgcgacttta ccctgaccaa tgagcggccg cacttgccgc aagatgtttt ctaaaattgc
     1321 attatccatg gcgactgcca ctttctactc ctggaccgca ggtctgaaaa gacctgcgag
     1381 tatatcagag ctgaatatgt cgcgtcagat ccggtctttc cacactgtct ggatattaca
     1441 gaattcgtgt aagccgaaat gggaaagctc acgaccaaag ccactctttt tcacgccacc
     1501 aaaggccact cgcgcgtcgc tggcacaata accattgata aacaccccac cgcattccag
     1561 acgtgccgcc atctgtctgg cctgtgtttc gtcggtggta aaaatggtcg ctgaaaggcc
     1621 gaactcacta tcattagcca gttccagcgc atgttctgca tctttcgcaa tggtgattgc
     1681 agcaacgggg ccaaacattt cttcccgaaa cgcggtcatt tctggggtaa cattcgccag
     1741 aaccgttggc ggatagtagt tacctgctcc agccatcttt tccccgccca gtaacaaacg
     1801 cgcaccctgc gccagggttt tctccacctg atgatgcagc tcatcacgta aatcaaaacg
     1861 agccattggt ccgagagcgt tctcttcatc acggggatcg cccattttca aggctgccgc
     1921 agctgacaca aaacgttcgg taaatgccga agcaattccc tcttcgataa taaagcgttt
     1981 tgccgctgca caaacctgtc cggtattctg ataacgtccg gctaccgccg ctttaaccgc
     2041 cagttccaga tcggcatcgt taagcacaat aaatggatcc gaaccgccca gttccagtac
     2101 gcattttttc agtgccgctc cagcacgaac acttccggtc accgtgacag cagcaatgcg
     2161 cgagtcttta atcatctgac tgacaccgtc gttgtcggca ttcagccagc catatacgcc
     2221 ttgcgggatt cccgcatctt taaacacttg ggcaatgagc tgagcacagc ccatcacatt
     2281 cggcgcatgt ttaagtaagt agccgttacc tgcaagaatg atgggaacag cgccacgcat
     2341 cacctgcctt tgatagttct tgcagctcaa atgccctata tagtgatgtt ccgatgactt
     2401 atgactatat ggggcaaata tggttacgcc agtaagcatc agcaattaca tatctcttcc
     2461 tgatgatttt cccgctcgta atattgcacc tcaagtaaaa gaagttttaa aggattttat
     2521 tgatgcactc agtaccataa tatgtgatga agagtggcgc acgtctttaa acaccaactc
     2581 agccacaaaa aagatattta acaaccttga taacttatca tatattcaga ggacttcttt
     2641 tcggggtaac gacacactat acaatgaaaa ggttcagttt aaactcactt atcctgtcaa
     2701 gaatggaaga cacaaagaaa atattgaatt tcaggtagta ataaatttaa gccctattta
     2761 tttagataat tttcgccatg atggagaaat taatattttt tgcgctccca acccaaagcc
     2821 tgtcactatg gggcgcgtat ttcagaccgg cgtcgagcgt gtgctctttc tgtttatgaa
     2881 tgattttatt gaacaatttc caatgatcaa ccttggcgcc cccatcaaaa gagcgcatac
     2941 accacatatt gaacccctgc ctccggatca ccataccgct gcagattact tacgccagtt
     3001 tgatttgctt gtcctgaatt ttatctctcg cggtaatttt gtcatactcc cccgattatg
     3061 gaataactct gaggttcaca gatggttcgt caataaagat cctaacttga tcaccgccat
     3121 tctcgacata acggacagcg aattaaaaga ggatttattg caaagcctga tggattcatt
     3181 aggttctaac aaacatgtac aacccgaagt ctgcatctgc ttcttatccc ttttagcaga
     3241 acaagaatct cctcattttc aggacttgtt tttatttttc gccaatatgt tattgcacta
     3301 tcaccaattt atgaatccca atgaaagtga tctgaatgac gtgttgatgc cagcatcatt
     3361 aagtgatgat aaaattatca aacatatggc gcgcaggacc ctcaaactgt ttgtaaaaaa
     3421 tgaaacgccg ccaaaagtta ctcacgaaga cctggtgaaa aacaggcctc gctcccctgt
     3481 cagaccacct atacccgcaa ccgccaaaac gccagacctc cctgaacgtc attaaaccgt
     3541 gatgttaccg actctctgac tcggaacaga atcagcgtca gagaaacgga aaacgcgatc
     3601 cagatcacaa atgcattgta ttcacatcat taaccgtttt aagatcattt catcactttt
     3661 tcgcaactca cccgataatc tgttatgaca acaaacactg tttcccgcaa agtggcgtgg
     3721 ctacgggtcg ttacgctggc agtcgccgcc ttcatcttca acaccaccga atttgtccct
     3781 gttggcctgc tctctgacat tgcgcaaagt tttcacatgc aaaccgctca ggtcggcatc
     3841 atgttgacca tttacgcatg ggtagtagcg ctaatgtcat tgccttttat gttaatgacc
     3901 agtcaggttg aacggcgcaa attactgatc tgcctgtttg tggtgtttat tgccagccac
     3961 gtactgtcgt ttttatcgtg gagctttacc gttctggtga tcagtcgcat tggtgtggct
     4021 tttgcacatg cgattttctg gtcgattacg gcgtctctgg cgatccgtat ggctccggcc
     4081 gggaagcggg cacaggcctt gagtttaatt gccaccggta cagcactggc gatggtctta
     4141 ggtttaccgc tcgggcgcat tgtggggcag tatttcggct ggcgaatgac cttcttcgcg
     4201 attggtattg gggcgcttgt tacccttttg tgcctgatta agttacttcc cttactgcct
     4261 agtgagcatt ccggttcatt gaaaagcctc ccgctattgt tccgccgccc ggcattgatg
     4321 agcatttatt tgttaactgt ggtggttgtc accgcccatt acacggcata cagctatatc
     4381 gagccttttg tgcaaaacat tgcgggattc agcgccaact ttgccacggc attactgtta
     4441 ttactcggtg gtgcgggcat tattggcagc gtgattttcg gtaaactggg taatcaatat
     4501 gcgtctgcgt tggtaagtac ggcgattgcg ctgttgctgg tgtgtctggc actgctgcta
     4561 cctgcggcga acagtgaaat acacctcggg gtgctgagta ttttctgggg gatcgcgatg
     4621 atgatcatcg ggcttggtat gcaggttaaa gtgctggcgc tggcaccaga tgctaccgac
     4681 gtcgcgatgg cgctattctc cggcatattt aatattggaa tcggggcggg tgcgttggta
     4741 ggtaatcagg tgagtttgca ctggtcaatg tcgatgattg gttatgtggg cgcggtgcct
     4801 gcttttgctg cgttaatatg gtcaatcatt atatttcgcc gctggccagt gacactcgaa
     4861 gaacagacgc aatagttgaa aggcccattc gggccttttt taatggtacg ttttaatgat
     4921 ttccaggatg ccgttaataa taaactgcac acccatacat accagcagga accccatcag
     4981 gcgggagatc gcttcaatgc cacccttgcc caccagcagc ataattgcgc cggagctgcg
     5041 taggcttccc cacaaaataa ccgccaccag gaaaaagatc agcggcggcg caaccatcag
     5101 tacccaatca gcgaaggttg aactctgacg cactgtggac gccgagctaa taatcatcgc
     5161 tatggttccc ggaccggcgg tacttggcat tgccagtggt acaaaggcaa tattggcact
     5221 gggttcatct tccagctctt ccgacttgct tttcgcctcc ggtgaatcaa tcgctttctg
     5281 ttgcggaaaa agcatccgaa aaccgataaa cgcgacgatt aagccgcctg caattcgcag
     5341 accgggaatc gaaatgccaa atgtatccat caccagttgc ccagcgtaat acgccaccat
     5401 catgatggca aatacgtaca ccgaggccat caacgactga cgattacgtt cggcactgtt
     5461 catgttgcct gccaggccaa gaaatagcgc gacagttgtt aatgggttag ctaacggcag
     5521 caacaccacc agccccaggc caattgcttt aaacaaatct aacattggtg gttgttatcc
     5581 tgtgtatctg ggttatcagc gaaaagtata aggggtaaac aaggataaag tgtcactctt
     5641 tagctaacct tgcatcgcat tgaccaaaac ttgaaccgat ttagcaaaac gtggcatcgg
     5701 tcaattcatt catttgactt atacttgcct gggcaatatt accccctaca actaattact
     5761 tgccagggca actaatgtga aaagtaccag cgatctgttc aatgaaatta ttccattggg
     5821 tcgcttaatc catatggtta atcagaagaa agatcgtctg cttaacgagt atctgtctcc
     5881 gctggatatt accgcggcac agtttaaggt gctctgctct atccgctgcg cggcgtgtat
     5941 tactccggtt gaactgaaaa aagtgttgtc ggtcgacctg ggagcactga cccgtatgct
     6001 ggatcgcctg gtctgtaaag gctgggttga aaggttgccg aacccgaatg ataagcgcgg
     6061 cgtactggta aaacttacca ccagcggcgc ggaaatatgc gaacaatgcc atcaattagt
     6121 tggccaggac ctgcatcaag aattaacaaa aaacctgacg gcggacgaag ttgcaacact
     6181 tgagcatttg cttaagaaag tcctgccgta aacaaaaaag aggtatgacg atgtccagac
     6241 gcaatactga cgctattacc attcatagca ttttggactg gatcgaggac aacctggaat
     6301 cgccactgtc actggagaaa gtgtcagagc gttcgggtta ctccaaatgg cacctgcaac
     6361 ggatgtttaa aaaagaaacc ggtcattcat taggccaata catccgcagc cgtaagatga
     6421 cggaaatcgc gcaaaagctg aaggaaagta acgagccgat actctatctg gcagaacgat
     6481 atggcttcga gtcgcaacaa actctgaccc gaaccttcaa aaattacttt gatgttccgc
     6541 cgcataaata ccggatgacc aatatgcagg gcgaatcgcg ctttttacat ccattaaatc
     6601 attacaacag ctagttgata acgtgacaac gtcactgagg caatcatgaa accactttca
     6661 tccgcaatag cagctgcgct tattctcttt tccgcgcagg gcgttgcgga acaaaccacg
     6721 cagccagttg ttacttcttg tgccaatgtc gtggttgttc ccccatcgca ggaacaacca
     6781 ccgtttgatt taaatcacat gggtactggc agtgataagt cggatgcgct cggcgtgccc
     6841 tattataatc aacacgctat gtagtttgtt ctggccccga catctcgggg cttattaact
     6901 tcccaccttt accgctttac gccaccgcaa gccaaataca ttgatataca gcccggtcat
     6961 aatgagcacc gcacctaaaa attgcagacc cgttaagcgt tcatccaaca atagtgccgc
     7021 acttgccagt ccgactacgg gcaccagtaa cgataacggt gcaacccgcc aagtttcata
     7081 gcgtcccagt aacgtccccc agatcccata accaacaatt gtcgccacaa acgccagata
     7141 catcagcgac aagatggtgg tcatatcaat agtaaccaga ctgtgaatca tggatgcgga
     7201 accatcgaaa atcagcgaga caacaaagaa gggaatgatt gggattaaag cgctccagat
     7261 taccagcgac atcaccgccg gacgcgttga gtgcgacatg atctttttat tgaagatgtt
     7321 gccacacgcc caactaaatg ctgcagccag ggtcaacata aagccgagca tcgccacatg
     7381 ctgaccgttc agactatctt cgattaacac cagtacgcca aaaatcgcta aggcgatccc
     7441 cgccaattgt ttgccatgca gtcgctcccc gaaagtaaac gcgccaagca cgatagtaaa
     7501 aaacgcctgt gcctgtaaca ccagcgaagc cagtccagca ggcataccaa agttaatggc
     7561 gcagaacaga aaggcaaact gcgcaaaact gatggttaat ccatacccca agagcaaatt
     7621 cagtggtact ttcggtcgtg caacaaaaaa gatggccgga aaagcgacca gcataaagcg
     7681 caaaccggcc agcatcagcg gtggcatgtt atgaagcccc actttgatga ccacaaaatt
     7741 tagcccccat acgaccacta ccagtagcgc caacacccca tcttttcgcg acattctacc
     7801 gcctctgaat ttcatctttt gtaagcaatc aacttagctg aatttacttt tctttaacag
     7861 ttgattcgtt agtcgccggt tacgacggca ttaatgcgca aataagtcgc tatacttcgg
     7921 atttttgcca tgcgtccgat ggttaataac aacgacgagg caagaagggc gctggtagag
     7981 cgtcgtaggg ataagttcat ttgtctgtcc ggcagtgcgt ttcgttatgt tttattttag
     8041 agatgtaaag aaatagcatg gctacgctgc catttatgac catttacttg agtcgccagt
     8101 acagcctgag tgtcgatcta atcggttatg cgatgacaat tgcgctcact attggcgtcg
     8161 tttttagcct cggttttggt atcctggcgg ataagttcga caagaaacgc tatatgttac
     8221 tggcaattac cgccttcgcc agcggtttta ttgccattcc tttagtgaat aacgtgacgc
     8281 tggttgtgct cttttttgcc ctcattaact gcgcctattc tgtttttgct actgtgctga
     8341 aagcctggtt tgccgacaat ctttcgtcca ccagcaaaac gaaaatcttc tcaatcaact
     8401 acaccatgct aaacattggc tggaccatcg gtccgccgct cggcacgctg ttggtgatgc
     8461 agagcatcaa tctgcccttt tggctggcag ctatctgttc cgcgtttccc atgcttttca
     8521 ttcaaatatg ggtaaagcgc agcgagaaaa tcatcgccac ggaaacaggc agtgtctggt
     8581 cgccgaaagt tttattacaa gataaagcac tgttgtggtt tacctgctct ggttttctgg
     8641 cttcttttgt aagcggcgca tttgcttcat gcatttcaca atatgtgatg gtgattgctg
     8701 atggggattt tgccgaaaag gtggtcgcgg ttgttcttcc ggtgaatgct gccatggtgg
     8761 ttacgttgca atattccgtg ggccgccgac ttaacccagc taacatccgc gcgctgatga
     8821 cagcaggcac cctctgtttc gtcatcggac tggtcggttt tattttttcc ggcaacaacc
     8881 tgctattgtg gggtatgtca gctgcggtat ttactgtcgg tgaaatcatt tatgcgccgg
     8941 gcgagtatat gttgattgac catattgcgc cgccaggaat gaaagccagc tatttttccg
     9001 cccagtcttt aggctggctt ggtgccgcga ttaacccatt agtgagtggc gtagtgctaa
     9061 ccagcctgcc gccttcctcg ctgtttgtca tcttagcgtt ggtgatcatt gctgcgtggg
     9121 tgctgatgtt aaaagggatt cgtgcaagac cgtgggggca gcccgcgctt tgttgattta
     9181 agtcgaacac aataaaaatt taattcagcc ttcgtttagg ttatctctgc taatatcttt
     9241 ctcattgaga tgaaaattaa ggtaagcgag gaaacacacc acaccataaa cggaggcaaa
     9301 taatgctggg taatatgaat gtttttatgg ccgtactggg aataatttta ttttctggtt
     9361 ttctggccgc gtatttcagc cacaaatggg atgactaatg aacggagata atccctcacc
     9421 taaccggccc cttgttacag ttgtgtacaa ggggcctgat ttttatgacg gcgaaaaaaa
     9481 ccgccagtaa accggcggtg aatgcttgca tggatagatt tgtgttttgc ttttacgcta
     9541 acaggcattt tcctgcactg ataacggatc gttgacacag tagcatcagt tttctcaatg
//
LOCUS       DAEEGA010000123         9493 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEEGA010000123 DAEEGA010000000
VERSION     DAEEGA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.123"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..190)
                     /locus_tag="KLH46_003585"
     CDS             complement(<1..190)
                     /locus_tag="KLH46_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HBE0380133.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="KLH46_003586"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="KLH46_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBE0380134.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="KLH46_003587"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="KLH46_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBE0380135.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="KLH46_003588"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="KLH46_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBE0380136.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="KLH46_003589"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="KLH46_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="KLH46_003590"
     CDS             complement(3262..3381)
                     /locus_tag="KLH46_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBE0380137.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="KLH46_003591"
     CDS             complement(3342..3527)
                     /locus_tag="KLH46_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380138.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="KLH46_003592"
     CDS             3628..3801
                     /locus_tag="KLH46_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380139.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="KLH46_003593"
     CDS             3803..4147
                     /locus_tag="KLH46_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBE0380140.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="KLH46_003594"
     CDS             4151..4486
                     /locus_tag="KLH46_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBE0380141.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="KLH46_003595"
     CDS             complement(4543..6864)
                     /locus_tag="KLH46_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE0380142.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVYNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="KLH46_003596"
     CDS             complement(6981..7190)
                     /locus_tag="KLH46_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380143.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="KLH46_003597"
     CDS             complement(7941..9464)
                     /locus_tag="KLH46_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBE0380144.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2659 a         2203 c         2080 g         2551 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ttggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattataaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc ataaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgcagg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa tac
//
LOCUS       DAEEGA010000124         9348 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEEGA010000124 DAEEGA010000000
VERSION     DAEEGA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.124"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(91..981)
                     /gene="malG"
                     /locus_tag="KLH46_003598"
     CDS             complement(91..981)
                     /gene="malG"
                     /locus_tag="KLH46_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBE0380145.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(996..2540)
                     /gene="malF"
                     /locus_tag="KLH46_003599"
     CDS             complement(996..2540)
                     /gene="malF"
                     /locus_tag="KLH46_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBE0380146.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYVWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2694..3884)
                     /gene="malE"
                     /locus_tag="KLH46_003600"
     CDS             complement(2694..3884)
                     /gene="malE"
                     /locus_tag="KLH46_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBE0380147.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4249..5364
                     /gene="malK"
                     /locus_tag="KLH46_003601"
     CDS             4249..5364
                     /gene="malK"
                     /locus_tag="KLH46_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBE0380148.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5436..6776
                     /gene="lamB"
                     /locus_tag="KLH46_003602"
     CDS             5436..6776
                     /gene="lamB"
                     /locus_tag="KLH46_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBE0380149.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPSAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            6928..7848
                     /gene="malM"
                     /locus_tag="KLH46_003603"
     CDS             6928..7848
                     /gene="malM"
                     /locus_tag="KLH46_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBE0380150.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELPLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSAERLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            8077..9348
                     /locus_tag="KLH46_003604"
                     /pseudo
     CDS             8077..9348
                     /locus_tag="KLH46_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT         2413 a         2296 c         2321 g         2318 t
ORIGIN      
        1 aaggcgctcc gtggagcgcc gaataatagt cacaagttgg gataacgtaa gttgagggtg
       61 cagcggcata acattggcag aacaacatct ttaacctttc acaccacctg ccgtcaggcc
      121 gttgaccagc cagcgttgag ccagcaagaa gacgatggtg atcggtaatg cagacatcac
      181 ggcagcggcg gcaaagtcac cccacaggta gttttgcggg ttgaggtatt gctgcatccc
      241 cacggccagg gtgtagctgt ttacgtcacg cagtaacagc gacgcgaccg gaacttcagt
      301 aatggcagcg ataaacgaca ggatgaatac caccgccaga atcggtactg acagcggcaa
      361 caggacaagg cggaaggcct gccacggtgt cgcaccatcc agcgcagccg cttcttccag
      421 cgaactgtcg atggtttcga aatagccttt gatggtccag acatgcagcg caatcccacc
      481 cagatacgcg aaaattacgc cgccgtgagt attcaggcca atgaatggaa tgtactcacc
      541 cagacgatca aacaacgcat acaacgcgac cagtgaaagt actgccggga acatctggaa
      601 aatcagcatt cctttcagca gcgtcgcttt gcctggaaag cgcatacggg cgaaagcgta
      661 ggcgcaggtg gtggagagcg ccacaatgcc aatcgcggaa atcccagcga cctttaccga
      721 gttccacagc cacagcagta ccgggaatgg cggtggcgta atgcgaccat cagcctgttc
      781 aacgctaaaa cctaacgcca gtttccagtg atcccaggag atttgctccg ggatcaggct
      841 accggtcgcg aagtttccct gacgcagcga gatagcgacg accatcagca gcgggaacat
      901 aatcgccgcg ataaaaagta gcagtagcag gtgagtaata aacaaacgtg ctttttgcga
      961 tttcggttgg accattgcca tttttgttat ctcccttaat caaacttcat tcgcgtggct
     1021 ttcaggttca ctatcgccag cgcacccacc agcaggaaga tcagcgtggc aattgctgcc
     1081 gccagaccga agtcctgacc cccgccgcct tcaaaagcga tacggtaggt gtagttaaca
     1141 agcaggtcgg tataaccggc tggcgtggtc gtgccaagac gatccgggcc gccgttggtt
     1201 aacagctgaa tcagcacgaa gttgttaaag ttaaaggcga agctggcgat catcagcggc
     1261 gtcagcggtt taatcagcag cggcagcgta atcttaaaga agttctggaa cggacctgcg
     1321 ccatccattg ctgaggcttc atacaaatcg tccggaatcg ctttcagcaa gcccatgcag
     1381 aggatcatca tgtacggata acccagccag gtattgacga taatcagcat cgtgcgagcg
     1441 gtggtcggat cgctgaacca ggcgggcttc acgccaaaca gcgcgctcaa catcatgttg
     1501 atttcaccga agctctggtt aaacaatcct ttgaaaatca agattgaaat gaacgatggc
     1561 accgcgtagg gcagaatcag caggacgcga tagaccgctt tgccgcgcaa cgcttcccac
     1621 tgcaccagac atgccagaac catgccgacc gccaccgtta aaaagacagt gatcagcgag
     1681 aacaccacgg tccagacgaa aatggcgagg aacggtttct gaatgccttc gtcggtaaag
     1741 acgcgggtaa agtttttcca gccggtggtc acggtgtaac cggggcttag cttttcatcc
     1801 ccccagttgc cgtcggcggt aatggactgg taaaagccaa tttggttatt cggacgatat
     1861 ttcacgccac tctgattatt cgtcaacgtg ccgtcaccgt cgagtgtgta gagcggctgc
     1921 gtgccagaaa actggcgcag ggagctcatc atcactttgt tgccatccgg cagaatggcg
     1981 gtaatgtcac tcagcgcctg acgattctgg gtaatcacgc gcagattcgc gcgttcgcct
     2041 tcgggctggg cggtcgtttc tttcagttgc agtttttgct cgccgccaaa tttaaacgcg
     2101 tcggagaggt aatttttgcc ggtttcgccg tcgctgagcg ccagttgcca ctcatcgccc
     2161 gccgggtaaa gaccaaagtt ataggttttg cctgcttgcc aggagcgatc taacaacact
     2221 tcctgcgcac gttcaaaagt cagctggtta gtgctgctgt agttggtgaa ggcaatggcg
     2281 atggtgcaga ccagagggaa gaggacgaat aatcccattc cagccattcc cgggtaaaca
     2341 tagcgccaga cgtaggcttt acgattggcg aaaatataca gccccgctga actcaatatc
     2401 agcgtggtaa tggcgaacag gtattcccct tgtgcgtaca ttaaaacaac aaggtaaccc
     2461 accagcaggc cgagcagacc tagcactgac catttcagcg cgtcgctttg ccaccaatgt
     2521 ttctttttaa tgacatccat ggggttcttc ctcattccag gacggataag gctttcacgc
     2581 cttatccgac aacaactgcc tgatgcgaca ctgatgcgtc ttatcaggcc tacatgcgtt
     2641 tcagttttgt aggccggaca aggcgttcac gccgcatccg gcatttcaca gcattacttg
     2701 gtgatacgag tctgcgcgtc tttcagggct tcatcgacag tctgacgacc gctggcggcg
     2761 ttgatcaccg cagtacgtac ggcataccag aaagcggaca tctgcgggat gttcggcatg
     2821 atttcacctt tctgggcgtt ttccatggtg gcggcaatac gtggatcttt cgccaactct
     2881 tcctcgtaag acttcagcgc tacggcaccc agcggtttgt ctttattaac cgcttccaga
     2941 ccttcatcag tcagcagata gttttcgagg aactctttcg ccagctcttt gttcggactg
     3001 gcggcgttaa taccggtgct cagcacgcca acgaacggtt tagatggttg acccttgaag
     3061 gtcggcagta ccgttacacc ataattcact ttgctggtgt cgatgttgga ccatgcccac
     3121 gggccgttga tggtcatcgc tgtttcgcct ttattaaagg cagcttctgc gatggagtaa
     3181 tcggtgtctg cattcatgtg tttgttttta atcaggtcaa ccaggaaggt cagacccgct
     3241 ttcgcgccag cgttatccac gcccacgtct ttaatgtcgt acttgccgtt ttcatacttg
     3301 aacgcataac ccccgtcagc agcaatcagc ggccaggtga agtacggttc ttgcaggttg
     3361 aacatcagcg cgctcttacc tttcgctttc agttctttat ccagcgccgg gatctcttcc
     3421 caggtttttg gcgggttcgg cagcaggtct ttgttataaa tcagcgataa cgcttcaaca
     3481 gcgattgggt aagcaatcag cttgccgttg taacgtacgg catcccatgt aaacggatac
     3541 agcttgtcct ggaacgcttt gtccggggtg atttcagcca acaggccaga ttgagcgtag
     3601 ccaccaaagc ggtcgtgcgc ccagaagata atgtcagggc catcgccagt tgccgcaacc
     3661 tgagggaatt tctcttccag tttatccgga tgctcaacgg tgactttaat tccggtatct
     3721 ttctcgaatt tcttaccgac ttcagcgaga ccgttatagc ctttatcgcc gttaatccag
     3781 attaccagtt taccttcttc gattttggcg agagccgagg cggaaaacat catcgtcgtt
     3841 aatgcggata atgcgaggat gcgtgcacct gtttttattt tcataatcta tggtccttgt
     3901 tggtgaagtg ctcgtgaaaa cacctaaacg gactctagtt tctttatacg gcaacctctt
     3961 tccatcctcc ttgcccctac gccccaccgt tgctttgtgt gatctctgtt acagaattgg
     4021 cggtaatgtg gggatgcgca cataaaatcg ccacgatttt tgcaagcaac atcacgaaat
     4081 tccttacatg acctcggttt agttcacaga agccgcgttc tcatcctccc gcctcctccc
     4141 ccataaaaaa gccagggggt ggaggattta agccatctcc tgatgacgca tagtcagccc
     4201 atcatgaatg ttgctgtcga tgacaggttg ttacaaagag agaagggcat ggcgagcgta
     4261 cagctgcaaa atgtaacgaa agcctggggc gaggtcgtgg tatcgaaaga tatcaatctc
     4321 gatatccatg aaggtgaatt cgtggtgttt gtcggaccgt ctggctgcgg taaatcgact
     4381 ttactgcgca tgattgccgg gcttgagacg atcaccagcg gcgacctgtt catcggtgag
     4441 aaacggatga atgacactcc gccagcagaa cgcggcgttg gtatggtgtt tcagtcttac
     4501 gcgctctatc cccacctgtc agtagcagaa aacatgtcat ttggcctgaa actggctggc
     4561 gcaaaaaaag aggtgattaa ccaacgcgtc aaccaggtgg cggaagtgct gcaactggcg
     4621 catttgctgg atcgcaaacc gaaagcgctc tccggtggtc agcgtcagcg cgtggcgatt
     4681 ggccgtacgc tggtggccga gccaagcgta tttttgctcg atgaaccgct ctccaacctc
     4741 gatgctgcac tgcgtgtgca aatgcgtatc gaaatctccc gtctgcataa acgcctgggc
     4801 cgcacaatga tttacgtcac ccacgatcag gtcgaagcga tgacgctggc cgacaaaatc
     4861 gtggtgctgg acgccggtcg cgtggcacag gttgggaaac cgctggaact gtaccactat
     4921 ccggcagacc gttttgtcgc cggatttatc ggttcgccaa agatgaactt cctgccggta
     4981 aaagtgaccg ccaccgcaat cgatcaagtg caggtggagc tgccgatgcc aaatcgtcaa
     5041 caagtctggc tgcctgttga aagccgtgat gtccaggttg gagccaatat gtcgctgggt
     5101 attcgcccgg aacatctact gccgagtgat atcgctgacg tcatccttga gggtgaagtt
     5161 caggtcgtcg agcaactcgg caacgaaacc caaatctata tccagatccc ttccattcgt
     5221 caaaacctgg tgtaccgcca gaacgacgtg gtgttggtag aagaaggtgc cacattcgct
     5281 atcggcctgc cgccagagcg ttgccatctg ttccgtgagg atggcactgc atgtcgtcga
     5341 ctgcataagg agccgggcgt ttaagcaccc cacaaaacac acaaagcctg tcacaggtga
     5401 tgtgaaaaaa gaaaagcaat gactcaggag atagaatgat gattactctg cgcaaacttc
     5461 ctctggcggt tgccgtcgca gcgggcgtaa tgtctgctca ggcaatggct gttgatttcc
     5521 acggctatgc acgttccggt attggctgga caggtagcgg cggtgaacaa cagtgtttcc
     5581 agactaccgg tgctcaaagt aaataccgtc ttggcaacga atgtgaaact tatgctgaat
     5641 taaaattggg tcaggaagtg tggaaagagg gcgataagag cttctatttc gacactaacg
     5701 tggcctattc cgtcgcgcaa cagaatgact gggaagctac cgatccggcc ttccgtgaag
     5761 caaacgtgca gggaaaaaac ctgatcgaat ggctgccagg ttccaccatc tgggcaggta
     5821 agcgcttcta ccaacgtcat gacgttcata tgatcgactt ctactactgg gatatttctg
     5881 gtcctagtgc cggtctggaa aacatcgatg ttggcttcgg taaactctct ctggcagcaa
     5941 cccgctcctc tgaagctggt ggttcttcct cttttgccag caacaatatt tatgactata
     6001 ccaacgaaac cgcgaacgac gttttcgatg tgcgtttagc gcagatggaa atcaacccgg
     6061 gcggcacatt agaactgggt gtcgactacg gtcgtgccaa cctgcgtgat aactatcgtc
     6121 tggttgatgg cgcatcgaaa gacggctggt tattcactgc tgaacatact cagagtgtcc
     6181 tgaagggctt taacaagttt gttgttcagt acgctactga ctcgatgacc tcgcagggta
     6241 aaggtctgtc gcagggttct ggcgtcgcgt ttgataacga aaaatttgcc tacaatatca
     6301 acaacaacgg tcacatgctg cgtatcctcg accacggtgc gatctccatg ggcgacaact
     6361 gggacatgat gtacgtgggt atgtaccagg atatcaactg ggataacgac aacggcacca
     6421 agtggtggac cgttggtatt cgcccgatgt acaagtggac gccaatcatg agcaccgtga
     6481 tggaaatcgg ctacgacaac gtcgaatccc agcgcaccgg cgacaagaac aatcagtaca
     6541 aaattaccct cgcacaacaa tggcaggctg gcgacagcat ctggtcacgc ccggctattc
     6601 gtgtcttcgc aacctacgcc aagtgggatg agaaatgggg ttacgactac aacggcgata
     6661 gcaaggttaa cccgaactac ggcaaagccg ttcctgctga tttcaacggc ggcagcttcg
     6721 gtcgtggcga cagcgacgag tggaccttcg gtgcccagat ggaaatctgg tggtaatagc
     6781 aaaacctggg ccggataagg cgtttacgcc gcatccggca taaaaacagg ttgtcattat
     6841 ctgaaagggg cgaaagcccc tctgattatc gggtttagcg cgctattgcc tggctaccgc
     6901 tgagctccag attttgaggt gaaaacaatg aaaatgaata aaagtctcat cgccctctgt
     6961 ttatcagcag ggttactggc aagcgcgcct ggaattagcc ttgccgatgt taactacgta
     7021 ccgcaaaaca ccagcgacgc gccagccatt ccatctgccg cgctgcaaca actcacctgg
     7081 acaccggtcg atcaatctaa aacccagacc acccaactgg cgaccggcgg ccaacaactg
     7141 aacgttcccg gcatcagtgg tccggttgct gcgtacagcg tcccggcaaa cattggcgaa
     7201 ctgcccctga cgctgaccag cgaagtgaac aaacaaacca gcgtttttgc gccgaacgtg
     7261 ctgattcttg atcagaacat gaccccatca gccttcttcc ccagcagtta tttcacctac
     7321 caggaactag gcgtgatgag tgcagagcgg ctggaaggcg ttatgcgcct gacaccggcg
     7381 ttggggcagc aaaaacttta tgttctggtc tttaccacgg aaaaagatct ccagcagacg
     7441 acccaactgc tcgacccggc taaagcctat gccaaaggcg tcggtaactc gatcccggat
     7501 attcccgatc cggttgctcg tcataccagc gatggcttac tgaaactgaa agtcaaaacg
     7561 aactccagct ccagcgtgtt ggtaggacca ctatttggtt cttccgctcc agctccggtt
     7621 acggtaggca acacggcggc accagctgtg gctgcacctg ctccggtgcc ggtgaagaaa
     7681 agcgagccga tgctcaacga cacggaaagt tattttaata ccgcgatcaa aaacgctgtc
     7741 gcgaaaggtg atgttgataa ggcgttaaaa ctgcttgatg aagctgaacg cctgggatcg
     7801 acatctgccc gttccacctt tatcagcagt gtaaaaggca aggggtaatt acgccccaca
     7861 gtgctgattt tgcaacaact ggtgcgtctc ctggcgcacc tttttttatg cttccttcct
     7921 gggatatgag cgatttttta tagtacttca cttcttcttc actaagaatg tccattattt
     7981 caatgcatta tcagagattc ttttcctttc gccggtagtg tctggacgct caggctactt
     8041 ttccaggtta ttttatttct gtattacaga ggttttatga taggtcatat cacaaattct
     8101 ggcggcaata actctttgat gaaacatgat gtggtgcaag gaaataatat agtagatctt
     8161 gatttactac gtaatttcaa tggggtgaca ggtttaaaca gagattactt tatttatatc
     8221 agtgatatat ttttaaatat aaaacaacgg aacgaaaaaa atcatgcaat aaatatgtta
     8281 cgtgaagtct caattagtaa tgatactata agtgtaaaat tctacagaaa tgaaaaaata
     8341 gaatgcgctt gtaattttct gatggataaa gatgcgcagg ggtatatcga cctgtctgat
     8401 tttgatttaa caaattgtca ttttaaaggt gacgttattt ctaaggtgtc ttttttatca
     8461 tcaaatctac aacatgtaac tttcgaatgt aaagaaattg agaattgcaa ttttactaag
     8521 gcaacagttg ataatgtcat atttaaatgt cgacgtttac acaatgtgat ttttctcaaa
     8581 acgagtggcg aatgtgttga ttttagccaa aatattcttg atacagttga cttctcgcag
     8641 agtcaacttg gtcatagtaa ttttcgcgaa tgtcagatta gaaattcaaa cttcgataat
     8701 tgttatcttt acgcttcgca cttcaccaga gcagagtttc tgtctgccaa agaaatatca
     8761 tttattaaat cgaatttgac agctgttatg tttgatcatg tgcgaatgtc gacagggaat
     8821 ttttaagatt gcattacaga acaattggaa ttaactattg attattcaga tatatttggg
     8881 aatgaagatc tcgatggtta tatcaataac attataaaaa tgattgatac attgccagat
     8941 aatgcaatga tattgaaatc cgttctggca gtaaaactgg tgatgcaatt aaaaattctc
     9001 aatattgtta ataaaaactt tattaagaat atgaagaaaa catttagcca ttgtccttat
     9061 ataaaagatc caattatacg tagttatata cattctgggg aagatgacaa gttcgataat
     9121 tttatgcgtc aacatcgatt cagtaaggtg aatttcgata cccaacagat gatccatttt
     9181 attaacagat ttaatatgaa taaagggctg gttgataaaa ataacaattt ttttatccaa
     9241 cttatcgatc aggccttacg atcaacggat gatatgatca aagcaaatgc ctggtatctt
     9301 tataaagagt ggattcgtag tgatgatgtt tcacctatat ttatagaa
//
LOCUS       DAEEGA010000125         9293 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEEGA010000125 DAEEGA010000000
VERSION     DAEEGA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.125"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="KLH46_003605"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="KLH46_003605"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBE0380151.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..3047
                     /locus_tag="KLH46_003606"
     CDS             1647..3047
                     /locus_tag="KLH46_003606"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBE0380152.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            3417..4241
                     /gene="gadX"
                     /locus_tag="KLH46_003607"
     CDS             3417..4241
                     /gene="gadX"
                     /locus_tag="KLH46_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBE0380153.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            4609..5337
                     /gene="gadW"
                     /locus_tag="KLH46_003608"
     CDS             4609..5337
                     /gene="gadW"
                     /locus_tag="KLH46_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBE0380154.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDANTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(5700..8813)
                     /gene="mdtF"
                     /locus_tag="KLH46_003609"
                     /pseudo
     CDS             complement(5700..8813)
                     /gene="mdtF"
                     /locus_tag="KLH46_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(8838..9095)
                     /locus_tag="KLH46_003610"
                     /pseudo
     CDS             complement(8838..9095)
                     /locus_tag="KLH46_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         2428 a         2162 c         2279 g         2424 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
     2041 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa
     2101 cggataaacc aaacctagtg tgcggtccgg tacaaatctg ctggcataaa ttcgcccgct
     2161 actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt atggacccga
     2221 aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga
     2281 cctacaccgg taactatgag ttcccacaac cgctgcacga tgcgctggat aaattccagg
     2341 ccgacaccgg tatcgacatc gacatgcaca tcgacgctgc cagcggtggc ttcctggcac
     2401 cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg
     2461 cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg
     2521 aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctgggtggt caaattggta
     2581 cttttgccat caacttctcc cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc
     2641 tgcgcctcgg tcgtgaaggc tataccaaag tacagaacgc ctcttaccag gttgccgctt
     2701 atctggcgga tgaaatcgcc aaacaggggc cgtatgagtt catctgtacg ggtcgcccgg
     2761 acgaaggcat cccggcggtt tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc
     2821 tgtacgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc ttcactctcg
     2881 gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg
     2941 actttgctga actgttgctg gaagactaca aagcctccct gaaatatctc agcgatcacc
     3001 cgaaactgca gggtattgcc cagcagaaca gctttaaaca cacctgataa cataacgttg
     3061 taaaaaccga atgcccagcc tttaaaaaaa cagctgggca ttcggttgct tattaatcgc
     3121 aataatatat tggctgtcta ttcatcgtgt tgataagata tatgttatat gaatgtttat
     3181 taaatgtagc gattatcttt aatgtcacga aaagggatta tttgcttgct attaatttca
     3241 ctgcgttctt aaacacgttt ttgtattata aatccagtca tcctgcccgt agaacatcac
     3301 acattatcat cctgttctcc cgcttcgttt aaatttattt atcaatcaat ttgacttaag
     3361 agggcggcgt gctacattaa ttaacagtaa tatgtttatg taatattaag tcaactatgc
     3421 aatcattaca tgggaattgt ctaattgcgt acgcaagaca taaatatatt ctcaccatgg
     3481 ttaatggtga atatcgctat tttaatggcg gtgacctggt ttttgcggat gcaagccaaa
     3541 ttcgagtaga taagtgtgtt gaaaattttg tattagtatc aagggatacg ctttcattat
     3601 ttctgccgat gctcaaggag gaggcattaa atcttcatgc acataaaaaa atttcttcat
     3661 tactcgttca tcactgtagc agagatattc ctgtttttca ggaagttgcg caactatcgc
     3721 agaataagaa tcttcgctat gcagaaatgc tacgtaaaag agcattaatc tttgcgttgt
     3781 tatctgtttt tcttgaggat gagcacttta taccgctgct tttgaacgtt ttgcaaccga
     3841 acatgcgaac acgagtttgt acggttatca ataataatat cgcccatgag tggacactag
     3901 cccgaatcgc cagcgagctg ttgatgagtc caagcctgtt aaagaaaaaa ttgcgcgaag
     3961 aagagacatc atattcacag ttgcttactg agtgtagaat gcaacgtgct ttgcaactta
     4021 ttgttataca tggcttttca attaagcgag tcgcagtgtc ctgtggatat cacagcgtgt
     4081 cgtatttcat ttacgtcttt cgaaattatt atgggatgac gcccacagag tatcaggagc
     4141 gatcggcgca gggattgccg aaccgtgact cggcggcaag tattgttgcg caagggaatt
     4201 tttacggcac taaccgttct gcggaaggaa taagattata gagttttact cagacataaa
     4261 aaaaacccgg cataggggac cgggaagagg atagtctgcc gtctccagac taataaaccg
     4321 ttataacact ccctgttggc acgggaaact ttgtgctctc agtaagttaa atataacttt
     4381 tactggaaat aagatcagcc atttttttat aaacataagc tatacgctgt gcgaaaatat
     4441 attcgtgctg catttactta ttatcaatta actgttatgc aaaactactt tgtggataaa
     4501 ttttggtcct accaaatctg gcagtttttg cgctaagaaa cagtctgtca tcatttcatt
     4561 agtatactga aattgaaata atcgcagtat gaaatataag ggataatcat gactcatgtc
     4621 tgctcggtga tcctcattcg tcgttcattc gatatttatc atgaacagca aaaaatatcg
     4681 ctgcataacg agagtatcct gctgctggag aaaaatttgg cagacgattt tgcgttttgt
     4741 tcaccggata cgcgacgact ggatatcgat gagctgacag tttgccatta cttacaaaat
     4801 attcgtcagc taccacgcaa tttagggtta catagcaaag accgtctgtt aattaaccag
     4861 tcacccccca tgccgctggt gacggcgatt tttgatagct tcaatgaatc cggggtaaat
     4921 tcaccgatac tgagcaatat gctctacctt tcctgtttat cgatgttttc tcataagaaa
     4981 gaactgatcc ccttactttt caatagcatc agcactgttt caggaaaagt tgaacgcctt
     5041 attagctttg atatcgccaa acgttggtat ctgcgcgata tcgcggaaag aatgtatacc
     5101 agcgagagtc tcatcaaaaa aaagttgcag gatgcaaata cctgtttcag taaaatatta
     5161 ctcgcttcca ggatgtcgat ggccagacga ttactcgagt tacgtcaaat tcctctgcat
     5221 actattgcgg aaaaatgtgg ctatagcagt acgtcgtact ttataaacac atttcgacaa
     5281 tattatggtg taacgccaca tcagtttgcg caacattcgc caggtacctt ttcctgacat
     5341 attttgcatt tgaatattgg tcaggatctc acacctgctt catgtgaaac tcttccctga
     5401 tgatttctgc cgggctaccg gctagttctc tttcgcagtt tgccaggcgg tataggtatt
     5461 tttaggagga aaagactcgg catgtttggg attattaagc tgacaattca tacgattacg
     5521 ggaatgtggg tcagtattgt gttgttcaaa ttgatgacga atggctggag tggtttctac
     5581 tttcagtgct gtgtactaag cctggtattc ctgacggtta gctggttgtt gagtggtgaa
     5641 tggttagcag gaaagagtaa ggctgaacct tcacgttcaa ccttactctc atttacacgt
     5701 tacgcttttt taaagcgggc aaagagatgt tccactacaa cgaaaaagac cggaacgaag
     5761 taaattgcca gcactgttgc ggcaaacatc ccgcccatca cgccggtacc taccgcgttt
     5821 tgcgcgccag aaccggcacc atgactgata accagcggca gcacgccgag aataaaggcc
     5881 agagaggtca tcaggattgg gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc
     5941 ggcgttttcc cttctttctg catcatctca acggcaaatt cgacaatcag gatggcgttc
     6001 ttggcggaaa gcccgatggt ggtcagcaaa ccaacctgga agtagacgtc attacttaag
     6061 ccgcgcagat cggtggccag taatgcgcca acgacgccta acggaacaac caacatcacc
     6121 gagaacggaa ttgaccagct ctcatagagt gcggcgaggg cgaggaacac cacgaccagt
     6181 gaaatggcat acagtgcagg agcctggttt gaggataacg cttcctgata cgacagtccg
     6241 gtccatgagt agccgacgcc tgccggaagt ttagcgacca ggtctgccat aaatttcata
     6301 gcgtcaccgg tacttttccc ggccgccgct tcacctaaaa tctccattga cgggatgccg
     6361 ttgtagcgtt ccaatcgcgg tgaaccatag gtccattcgg tagacgagta ggcagaaagc
     6421 ggtgccatcg tgccagaggc gttgcgtaca taccattggt tgatgttatc cggcaacata
     6481 cggaacggcg tgcctgcctg gacatacact tttttcaccc gcccctggtt gaggaagtcg
     6541 ttcacgtagc tgctgccgaa ggcggtggaa attgtctggt tgatatcaga cagcgccaca
     6601 cccattgcct cagctttcgc ggcgttgacg ttcactttga acatcggcgt atcttccagg
     6661 ccgttcgggc gtaccccggt gacctggtca ggcgattgcg ctgccagtga taacagctcg
     6721 tttcgtgcct aggttagttt ttcgtgcccc aggttaccgt tgtccagcag ttccatatca
     6781 aaacctgacg cggtacccag ttcagccacc gcgggtaagt tgaacgggaa gacgacggct
     6841 ttattgatac tgcttaacgc aatcattgcc cgctgaatga tcgcggtaac cgagttttcc
     6901 tcaccgacac gttcagacca cggcttgaga ctgataaacg ccaggccgtt gttttgcccc
     6961 tggccgctga agccaaagcc gccaacggta aacaccgact gaacattatc tttctctttt
     7021 gtaagataat aatccgtcac ctgttgcagt actttcgtgg tgttaaccat ggtggcaccg
     7081 gaaggtaact gcgcggtggt cataaatacc ccctgatcct cttctggtaa gaaagaggtc
     7141 ggtgtacgca ggaacagcac cgccatcccg gcgcaaatca gcaggtagac caccatgtag
     7201 cgaccggtac aacgcaacag cgagcgggtg ctatcggtat agtgctgagt tgatttttca
     7261 aacagcgtgt tgaagcgtgc gaacagggcg ttaggtttgt gaccgccttc cggcgtggct
     7321 ttcagaatgg tggcgcacag ggcaggggtc aggctcattg ccacaaatac tgaaagccgc
     7381 atggaggaga tcagcgtgat ggagaactgg cggtagatct ccccggttgc accgctcata
     7441 aaggccatcg gcataaacac tgcagaaaga acaacggcaa tcccgaccag cgcacgttgg
     7501 atctgcccca tcgatttatg cgtcgcttcc ttcggcggta gcttatcttc cgcaatgaca
     7561 cgctcgacgt tctccaccac cacgatggcg tcatccacca gtaaccctat cgccagcacc
     7621 atcccgaaca tagtcaacgt gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc
     7681 ccgagaataa ccaccggtac ggcaattgtc gggatgatgg tggcacggaa attctgcaaa
     7741 aacagataaa tgaccaggaa gacgaggatg atagcctcaa ccagtgtttt gaaaacttcc
     7801 tgaatagaaa tttcgataaa cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg
     7861 gggaaatagg ctgataagcg gttcagctcc tctttgactg cccgcgaggt atccagggcg
     7921 tttgctccgg cagccagttt gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc
     7981 acggtggaat aatcttccgc cccaagttcg acgcgggcga catcacgcag cagcacttgc
     8041 gaaccatctt gctgaacttt caacaggatt ttgccaaatt cttccggcgt ttgcagacgc
     8101 gtctgcacaa tgatcgaggc gtttagttgc tggtctgccg cctgtggcat gccacccagt
     8161 tgaccaccag aaatctggtt gttttgcacc ttaatctggg aaataacatc ggaaggtacc
     8221 aggttatatt tattgagttt ttgcgggtcc agccagatac gcatggcgta ctcggaacca
     8281 aagagctgta cgctaccaac gcccgcggta cggcttagcg ggtctttgat attagacgct
     8341 acatagtccg cgatatcgta ctggttgagg ctgccgttat cagaaataaa cgccgctacc
     8401 atcaggatat tactgctcga cttatcgacg ctaatcccct gttgctgcac cgcttcaggt
     8461 aatgaaggca tagcgagttg cagtttattt tgcacctgaa cctgtgcgat atcaggagat
     8521 gtcccagtct cgaaggtcag agtgatagag gcattacccg ccgcatcact ggttgaagac
     8581 atgtacatca ggccatcaag cccattcata ttttgctcaa tcacctgagt gaccgagtct
     8641 tctaccgttt gcgcatcggc acccggatag gtagcgctga cggtaatggt cggtggcgca
     8701 atctgcggat actgcgcaac cggtaagttc atgatcgcca gaccacctgc aagcatcata
     8761 ataatggcaa gtacccaggc aaaaaccggg cgatcaataa aatagttagc catgaaagtc
     8821 cccttaagcc tgcaacgtta ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa
     8881 attgctcgtg ctttgatacc cggacgaatg cgttgcaaac cggaaacgat cacccgatcg
     8941 ccagcctgca agccagaggt gacaacccac tggtcgccga tggctttgct ggcttcaatt
     9001 tcgcgtagct gcacgacatc gtctttatcc agaatgagcg ccgttgcttt accctgggcg
     9061 ttgtgggtga cgccttcctg cggcaccagt aatataaagc gcgcaaaatt attcaagata
     9121 tttttgaaaa tcattcaaga taggctatcg cgtgtgatag caaaaacatt caagataaaa
     9181 atgcttgatt attaagcaat caggaatggg agtattaagt ttcgtgtttc cagtaaaacc
     9241 agagagtatt gaaatagcta tcacctaact ttatcaggcg atagctggta gat
//
LOCUS       DAEEGA010000126         9154 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEEGA010000126 DAEEGA010000000
VERSION     DAEEGA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.126"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..924
                     /locus_tag="KLH46_003611"
     CDS             <1..924
                     /locus_tag="KLH46_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0380155.1"
                     /translation="LGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAP
                     PALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRP
                     QLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAI
                     PFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAV
                     AFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDT
                     THSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1176..2093
                     /gene="oxyR"
                     /locus_tag="KLH46_003612"
     CDS             1176..2093
                     /gene="oxyR"
                     /locus_tag="KLH46_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBE0380156.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWENRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="KLH46_003613"
     CDS             complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="KLH46_003613"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBE0380157.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3797..4447
                     /gene="fabR"
                     /locus_tag="KLH46_003614"
     CDS             3797..4447
                     /gene="fabR"
                     /locus_tag="KLH46_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBE0380158.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4447..4806
                     /locus_tag="KLH46_003615"
     CDS             4447..4806
                     /locus_tag="KLH46_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBE0380159.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4846..5934)
                     /gene="trmA"
                     /locus_tag="KLH46_003616"
     CDS             complement(4846..5934)
                     /gene="trmA"
                     /locus_tag="KLH46_003616"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBE0380160.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRAQNLNVHLIGRATKTKIALD
                     QDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNG
                     NFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREF
                     NRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQ
                     THKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6048..6238
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6303..8147
                     /gene="btuB"
                     /locus_tag="KLH46_003617"
     CDS             6303..8147
                     /gene="btuB"
                     /locus_tag="KLH46_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBE0380161.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8092..8949
                     /gene="murI"
                     /locus_tag="KLH46_003618"
     CDS             8092..8949
                     /gene="murI"
                     /locus_tag="KLH46_003618"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBE0380162.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2094 a         2218 c         2469 g         2373 t
ORIGIN      
        1 ctgggtattg gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactggtat
       61 gcacaaaaag aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag
      121 gctatcgcac ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc
      181 gttatcatcg gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac
      241 cgggcgcagt ttgtcctgac tgacgaagaa cggacgtatc tctccgcgtc ggttaaaccg
      301 cgtccacagc tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg
      361 atgattttag gtttctctgg cgtcaattat accggttggc tttacattgc gtggttaccc
      421 ggttatttgc aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt
      481 cctttcctcg cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg
      541 aaaaaaggct acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg
      601 tcggcattag gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt
      661 atctcaatgg cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag
      721 gtgatggtgt cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt
      781 gtttttgctt cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt
      841 aatctggcgc tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt
      901 attgtcaaag atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag
      961 aataaaaaaa agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag
     1021 ttgagaaact ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc gctccgtttc
     1081 tgtgagcaat tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta
     1141 tcgccatgaa ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc
     1201 tggtggcatt ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc
     1261 agccgacgct tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg
     1321 agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc
     1381 gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggtgagacga
     1441 tgtccggacc gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata
     1501 ttatcccgat gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga
     1561 cccaccagtt actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg
     1621 tgaaagagag cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta
     1681 tctatgaaga tcacccgtgg gagaaccgcg aatgcgtact gatggccgat ctggcagggg
     1741 aaaaactgct gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct
     1801 ttgaagccgg ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca
     1861 acatggtggc ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc
     1921 gcaaacgcga tggggttgtt tatctgccat gcattaagcc ggaaccacgc cgcactattg
     1981 gcctggttta tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca
     2041 tccgcgcaag aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt
     2101 aacgcagcta cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag
     2161 tactcaatag tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt
     2221 tcggcagcgc gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac
     2281 aaaattttca gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac
     2341 tgggcgcggc ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc
     2401 acagagctga tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg
     2461 gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat
     2521 gccaggctcg gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga
     2581 tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac
     2641 gccagcgaat cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt
     2701 tttttacccg acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac
     2761 tcttcgttgt gacgaatcac tacgccactg ttccagaagt ggtaggagag agaatctgac
     2821 atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca
     2881 tccataccgc ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt
     2941 acatggcgcg gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg
     3001 gtgaaatcaa catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca
     3061 gcggttagtg tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca
     3121 aagcgagcgt tcccctgcaa tatttcacag tgattacgtt cgtaaaatcc ctgacgcatg
     3181 cgcgtttgtt gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg
     3241 agcagtcggg aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg
     3301 gcgtgacgga gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt
     3361 tgataacgct cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct
     3421 tcgccgccgg ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta
     3481 gggcttacct gttcttatac ataaaagcaa cagaatggta acattctatc gcgggtaagc
     3541 caattgatcc ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct
     3601 tacttttgcc ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag
     3661 gtttccgctg tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt
     3721 cacaatactg gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg
     3781 caaggattca gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc
     3841 gctggtggaa gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt
     3901 gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga
     3961 cgtagacgaa ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat
     4021 gcgccaggcg cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac
     4081 atttatggag ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc
     4141 cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga
     4201 acttgcggac tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc
     4261 cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga
     4321 acaacgtcgg caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc
     4381 ttattactgg tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga
     4441 cgagtaatga aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggt
     4501 ttatcgatta atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc
     4561 ccgattattt ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc
     4621 atgtcggtag gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac
     4681 agcacggtag ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc
     4741 tccgtcatta tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct
     4801 gagtaatcgg tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag
     4861 taatacgccg cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc
     4921 gaccttgtgc gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga
     4981 gatatacaga atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct
     5041 gcgcggtggg tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag
     5101 gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat
     5161 aatttgtacg ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg
     5221 tttggcgatt tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt
     5281 accgttgccg cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc
     5341 ccattccagc atctgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg
     5401 acgatagatc atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag
     5461 cgcgattttg gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag
     5521 tgcttccgct tcctgacgcc actcatcatc cagcttttta tggtacagca gggaaaccac
     5581 cgcctgatta ctcagcgtgg tgaggtaatc aatctggaac aacttgtggc gcagaacggg
     5641 attattacgc acacccgcaa tcatcgccgt catcaactgg ttgatgagtt cactggcggc
     5701 ggggaagcta tccacgcgga tgcggctttt ggtttgttga tcgaaaatga tgtgatacag
     5761 gtcatcgcca tcgtgccaga tgcggaactc cgcgcgcatc cggtaatgac tgaccggcga
     5821 gcgaaacact tccggaacca ggtcagaaaa cggtgccatc atactttgca aacgtaccac
     5881 tttttcggct aactgcgctt catactgttc tgttggaagg tgttcggggg tcatgatgta
     5941 tcctgaaaaa ttaaagtacg cggcgattgt agggattgct catcagatgt ccagatcttg
     6001 atgaattcct atttgtgagc tacgtctgga cagtaacttg ttacaacctg tagcatccac
     6061 ttgccggtcc tgtgagttaa tagggaatcc agtgcgaatc tagagctgac gcgcagcggt
     6121 aaggaaaggt gcgatgattg cgttatgcgg acactgccat ccggtgggaa gtcatcatct
     6181 cttagtatct tagatacccc tccaagcccg aagacctgcc ggccaacgtc gcatctggtt
     6241 ctcatcatcg cgtaatattg atgaaacctg cggcatcctt cttctattgt ggatgcttta
     6301 caatgattaa aaaagcttcg ctgctgacgg cgtgttccgt cacggcattt tccgcttggg
     6361 cacaggatac cagcccggat actctcgtcg ttactgctat ccgttttgaa cagccgcgca
     6421 gcactgtgct tgcaccaacc accgttgtga cccgtcagga tatcgaccgc tggcagtcga
     6481 cctcggtcaa tgatgtgctg cgccgtcttc cgggcgtcga tatcacccaa aacggcggtt
     6541 caggtcagct ctcatctatt tttattcgcg gtacaaatgc cagtcatgtg ctggtgttaa
     6601 ttgatggcgt ccgcctgaat ctggcggggg tgagtggttc tgccgacctt agccagttcc
     6661 ctattgcgct tgtccagcgt attgaatata tccgtggacc gcgctccgct gtttatggtt
     6721 ccgatgcaat aggcggggtg gtgaatatca tcacgacgcg cgatgaaccc ggaacggaaa
     6781 tttcagcagg gtggggaagc aatagttatc agaactatga tgtctctacg cagcaacaac
     6841 tgggggataa aacacgggta acgctgttgg gcgattatgc ccatactcat ggttatgatg
     6901 ttgttgccta tggtaatacc ggaacgcaag cgcagacaga taacgatggt tttttaagta
     6961 aaacgcttta tggggcgctg gagcataact ttactgatgc ctggagcggc tttgttcgcg
     7021 gctatggcta tgataaccgt accaattatg acgcgtatta ttctcccggt tcaccgttgc
     7081 tcgatacccg taaactctat agccaaagtt gggacgccgg gctgcgctat aacggcgaac
     7141 tgattaaatc acaactcatt accagctata gccatagcaa agattacaac tacgatcccc
     7201 attatggtcg ttatgattcg tcggcgacgc tcgatgagat gaagcaatac accgtccagt
     7261 gggcaaacaa tgtcatcgtt ggtcacggta gtattggtgc gggtgtcgac tggcagaaac
     7321 agactacgac gccgggtaca ggttatgttg aggatggata tgatcaacgt aataccggca
     7381 tctatctgac cgggctgcaa caagtcggcg attttacctt tgaaggcgca gcacgcagcg
     7441 acgataactc acagtttggt cgtcatggaa cctggcaaac cagcgccggt tgggaattca
     7501 tcgaaggtta tcgcttcatt gcttcctacg ggacatctta taaggcacca aatctggggc
     7561 aactgtatgg cttctacgga aatccgaatc tggacccgga gaaaagcaaa cagtgggaag
     7621 gcgcgtttga aggcttaacc gctggggtga actggcgtat ttccggatat cgtaacgatg
     7681 tcagtgactt gatcgattat gatgatcaca ccctgaaata ttacaacgaa gggaaagcgc
     7741 ggattaaggg cgtcgaggcg acagccaatt ttgataccgg accactgacg catactgtga
     7801 gttatgatta tgtcgatgcg cgcaatgcga ttaccgacac gccgttgtta cgccgtgcta
     7861 aacagcaggt gaaataccag ctcgactggc agttgtatga cttcgactgg ggtattactt
     7921 atcagtattt aggcactcgc tatgataagg attactcatc ttatccttat caaaccgtta
     7981 aaatgggcgg tgtgagcttg tgggatcttg cggttgcgta tccggtcacc tctcacctga
     8041 cagttcgtgg taaaatagcc aacctgttcg acaaagatta tgagacagtc tatggctacc
     8101 aaactgcagg acgggaatac accttgtctg gcagctacac cttctgaacc acgtcccacc
     8161 gtgctggtgt ttgactccgg cgtcggtggg ttgtcggtct atgacgagat ccggcatctc
     8221 ttaccggatc tccattacat ttatgctttc gataacgtcg ctttcccgta tggcgaaaaa
     8281 agcgaagcgt ttattgttga gcgagtggtg gcaattgtca ccgcggtgca agaacgttat
     8341 ccccttgcgc tggctgtggt cgcttgcaac accgccagta ccgtttcact tcctgcatta
     8401 cgcgaaaagt tcgacttccc ggttgttggt gtcgtgccgg cgattaaacc tgctgcacgt
     8461 ctgacggcaa atggcattgt cggattactg gcaacccgcg gaacagttaa acgttcttat
     8521 actcatgagc tgatcgcgcg tttcgctaat gaatgccaga tagaaatgct gggctcggca
     8581 gagatggttg agttggctga agcgaagctg catggcgaag atgtttctct ggatgcacta
     8641 aaacgtatcc tacgcccgtg gttaagaatg aaagagccgc cagataccgt tgtattgggt
     8701 tgcacccatt tccctctact acaagaagaa ctgttacaag tgctgccaga gggaacccgg
     8761 ctggtggatt ctggcgcagc gattgctcgc cgaacggcct ggttgttaga acatgaagcc
     8821 ccggatgcaa aatctgccga tgcgaatatt gccttttgta tggcaatgac gccagaagct
     8881 gaacaattat tgcccgtttt acagcgttac ggcttcgaaa cgctcgaaaa actggcagtt
     8941 ttaggctgat ttggttgaat gttgcgcggt cggaaaatta ttttaaattt cctcttgtca
     9001 ggccggaata actccctata atgcgccacc actgacacgg aacaacggca aacacgccga
     9061 cgggtcagcg gggttctcct gagaattccg acagagaaag caaaaataaa tgcttgactc
     9121 tgtagcggga aagcgtatta tgcacacccc gcgc
//
LOCUS       DAEEGA010000127         9031 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEEGA010000127 DAEEGA010000000
VERSION     DAEEGA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.127"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="KLH46_003619"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="KLH46_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBE0380163.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="KLH46_003620"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="KLH46_003620"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBE0380164.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="KLH46_003621"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="KLH46_003621"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBE0380165.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="KLH46_003622"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="KLH46_003622"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBE0380166.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="KLH46_003623"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="KLH46_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBE0380167.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /locus_tag="KLH46_003624"
     CDS             5562..6035
                     /locus_tag="KLH46_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBE0380168.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="KLH46_003625"
     CDS             6064..6606
                     /locus_tag="KLH46_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HBE0380169.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="KLH46_003626"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="KLH46_003626"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBE0380170.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYDKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="KLH46_003627"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="KLH46_003627"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBE0380171.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNVFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="KLH46_003628"
     CDS             8003..8260
                     /locus_tag="KLH46_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBE0380172.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="KLH46_003629"
     CDS             complement(8236..8790)
                     /locus_tag="KLH46_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBE0380173.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2262 a         2192 c         2250 g         2327 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 atggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatacagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatgataaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgttttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaaaaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagaga a
//
LOCUS       DAEEGA010000128         8989 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEEGA010000128 DAEEGA010000000
VERSION     DAEEGA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8989
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.128"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            146..2330
                     /gene="espL4"
                     /locus_tag="KLH46_003630"
                     /pseudo
     CDS             146..2330
                     /gene="espL4"
                     /locus_tag="KLH46_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="KLH46_003631"
                     /pseudo
     CDS             complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="KLH46_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="KLH46_003632"
                     /pseudo
     CDS             complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="KLH46_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="KLH46_003633"
                     /pseudo
     CDS             complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="KLH46_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7423..8352)
                     /gene="metA"
                     /locus_tag="KLH46_003634"
     CDS             complement(7423..8352)
                     /gene="metA"
                     /locus_tag="KLH46_003634"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBE0380174.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8509..8952
                     /locus_tag="KLH46_003635"
     CDS             8509..8952
                     /locus_tag="KLH46_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBE0380175.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2356 a         2168 c         2167 g         2298 t
ORIGIN      
        1 cgcaagatct cattccaccg aaacttttac ggctgcatta tgagattgag cgatttatga
       61 tattttttta gttgtaatca tattaaattt tttcactgtt ctgatagtta aaattcaaga
      121 catcaataaa caatgagata tttaaatgat tactcgtatt cctcgtagtt ctttctctgc
      181 aaatattaat aatacagccc agacaaatga acaccaaacc ctgagtgaat tgttttataa
      241 agaactcgag gataaatttt ctggcaagga gctggcgacg cctctattaa aaagcttctc
      301 agagaactgt agacaaaatg ttcgtcatat ctttagcaac aaggattttg tcattaaatt
      361 ttccacgtcc gtcttacaag ctgataagaa agaaattacg ataattaata aaaacgaaaa
      421 cacgacactc actcaaacca ttgctacaat atttgaagaa tacctaatgg aaattttacc
      481 tcaacgctca gacactcttg ataaacaaga attaaaccta aaatcagata gaaaagaaaa
      541 agaattccca agaattaaac ttaatggtca atgttatttc ccggggcgcc ccaaaaccgt
      601 atagtatacc gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac
      661 aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa
      721 tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg cgttgctgcc
      781 tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc aaatggcatc
      841 agtgttatgg cagccatatt actggtggat aaccatgctt tgacggtccg gctaagaata
      901 aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc taatgtaact
      961 aacgataaaa taagaattat gagcgaaagc aaagaggata ttaaacacta ttctctgatg
     1021 gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt tattaaccaa
     1081 tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgttaaa aggaactgtg
     1141 gatgaaataa cccctccatt atcaccagca acgatgaatt tgctaatggc aattggtcag
     1201 aatcaccaac tcacgcaact gatgattcag ctccagaaaa tgccagaact acatagaaca
     1261 gaaatgttga ctgcctataa tagtattaac ctccccggtt tatatttggc tataaattat
     1321 ggcaatgcgg atatcgttga gactattttc aattcattgt cagaaacagg atatgaaggg
     1381 ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa tggtttttct
     1441 ggattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat tctgaacgtc
     1501 ttacctaaac tggccacaac acatcattta gataatgaac aggtgtataa attcctgagt
     1561 gccaaaaata gaacgtccag ccatgtttta taccatgtta tggcgaatgg tgatgccgac
     1621 atgctgaaaa tttttttggt cgcgttacct ttgttaattc gcacatgtca tttgactaaa
     1681 gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccaag actatacctg
     1741 gcgatgcaaa atggacatag cgatatcgtg aaagttattc tcgaagcatt gccctgtctg
     1801 gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc taaaagtctt
     1861 gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt tattaatact
     1921 atttttaacg cattacccac tctttttaat acgtttaaat tcgataaaaa aatatgaagc
     1981 ccctcctcct ggcaaataat tctaatgaat atcccggttt gttttcagcg atacagcata
     2041 aacaacaaaa cgttgtagag acggtttatc ttgctttatc tgaccatgca cgcctgtttg
     2101 gatttaccgc tgaagatatt atggattttt ggcaacacaa agcgccacaa aaatactctg
     2161 cctttgagtt ggcttttgaa tttggtcacc gggttattgc tgaattaatc cttaatacat
     2221 taaataagat ggctgaaagc tttggcttta cggataaccc tcgatacatt gcggagaaaa
     2281 attatatgga agctttactc aaaaaagcat ctccccatac cgtacgctaa atctttgcct
     2341 gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt gtagtgcgcg
     2401 ccagtaatca gcgcggaaca ggtcggcgtg catctcttca aacagcggac caatacgcgg
     2461 gtcggcgcat agccagtggc gaaactcttc cgggaaaaca tcgcccggcg agacgctgta
     2521 ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc ggcgggatgt cgcggaaatt
     2581 cacttccgtc atgtagcaaa tttcatcgta atcataaaaa accacacgcc cgtgacgggt
     2641 gacaccgaag tttttaaaca gcatgtcgcc agggaaaatg ttagcagcgg caagctggcg
     2701 aatagcgtta ccgtattctt caatggcgtc gcgcaactgc tgaccttcca cttgttccag
     2761 ccagatattg agcggcacca tccgccgctc gattatagag atggcgaatt acaatctgct
     2821 caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt aataccgggg
     2881 aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc attcggccca
     2941 cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca gacatctctt
     3001 tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa
     3061 acaccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca ttacagccct
     3121 gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg cagccgatag
     3181 ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac tcgaccagcg
     3241 ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa acaatgctcg
     3301 cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc gtctggtgga
     3361 tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc agccaggcgg
     3421 ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg aggttgtctg
     3481 tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt ttattctgcc
     3541 agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc cagccgtgat
     3601 cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc tctggctgag
     3661 agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca
     3721 cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac agccgcgtgt
     3781 aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc
     3841 gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg tttttcatcg
     3901 cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt
     3961 cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt
     4021 ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga tgcggcgtaa
     4081 acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg
     4141 catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg gcctacagtc
     4201 agcaacgttt gtaggttgct tagaactgcg attcttcagt ggagccagtc agcgcggtga
     4261 ctgaagacgt gccgccctga ataatagtcg tcactttatc gaagtaacct gtacccactt
     4321 cctgctggtg agatacgaag gtatagccat ctttcgcggc ggcaaattcc ggctgctgca
     4381 ctttctcaac atagtgcttc ataccttcgc cctgcgcata agcgtttgcc aggtcgaaca
     4441 tgttaaacca catgctgtgg atacctgcca gggtgatgaa ctggaacttg tagcccatat
     4501 ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc gaggtttttc tgccagttga
     4561 acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg atagcttgtg
     4621 caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccagacc aggtcagcat
     4681 atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga gtacggaaga
     4741 agccttcact ggtacgctcg ccggtaataa attcgctgtc atacgggtcg caatctgagg
     4801 tgatcagatc cgccgcatca gcatcggtac gtgcaaccag cagggttgga acgcccgtca
     4861 cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt ggcactaaaa
     4921 ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg aagtgaactg
     4981 ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg acaccgccaa
     5041 aaccggcttc cgcatcggca acgatcggca ggaagtaatc gacatagcgc ggatcgcccg
     5101 gctcaatgcc cacggaccat tggatctgat cggcacgacg gaaggtgttg ttgatccgct
     5161 ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac atgctggccg
     5221 ccaggttagc gtccgccgct acctgccatc ccgacagata gactgcttca atacccgctt
     5281 tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gctgttgatg tagctttttt
     5341 cgactcaccg tgcagcagac gccacatttt cgctgcgccc agttgcgcca gcgtgcattc
     5401 aggattgact gaaccgcgta atttcaccac atcttccgca ctgtatgggc gagtaatgcc
     5461 ttcccaacgc ggttgagtcc actctttctg taattcttca atttgttgtg tacgggtttt
     5521 catgtgcaga tgctccatag ttatgtggtg gtttacgcta acaggcggta gcctggcagg
     5581 gtcaggaaat caattaactc atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg
     5641 tcaaaacggc cctgggagaa acgttcttcg cccagttcgc tggcaatgac tttcatctct
     5701 tcgcccagca tctggcggaa caaggctttg gtcaccggtt tgccattgct caacgttttt
     5761 tgatgatgga tccactgcca gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc
     5821 atcaggccat aaatcggcac gcagccgttg ccggagatcc acgcttcgat gtactgcaca
     5881 gccacgcgaa tgttagcgcg cataccttct tcggtgcgtt caccatcaca aggtgccagc
     5941 agctgatcgg cagtaatcgg cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg
     6001 gagccgagaa tgtcgttgaa taccgccatt gccgtgtccg caaggcctgg gtgagcgatc
     6061 catgtgccat cgtgaccgtt attggcttcc agcgctttat ccgcttttac tttgttgagc
     6121 acctggttat tacgctcttc atctttgctc ggaataaacg cggccatgcc gcccatcgca
     6181 aaagcaccgc gtttatggca ggttttaatc agcaggcgtg agtaagcatt caggaatggt
     6241 ttatccatcg tcacggcctg tctgtctggc aggacacgat cgggatagtt tttcaacgtt
     6301 ttgatatagc tgaagatgta atcccagcga ccgcagttca gaccaacaat atggtcgcgc
     6361 agcgcgtgaa ggatttcgtc catctggaac acggcgggca gcgtttcaat cagcaacgtc
     6421 gccttgatgg tgccgcgcgg cagattaaag cgatcttctg catagctgaa gacttcgctc
     6481 caccaggccg cttcctgcta ggactgggtt ttcggcagat agaaataggg accgctgccc
     6541 tttgccaaca gtgcctgata gttgtggaag aaatagagcg caaaatcaaa caggctaccg
     6601 gggattgcct caccacgcca ggtgacatgt ttttccggca agtgcagacc gcgtacccga
     6661 caaatcaaaa ccgctggatt gggcttgagc tgataaattt tgcctgcttc attggtatag
     6721 ctgatggtac cgttaaccgc atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag
     6781 tctggtgcca gtgaatcttc gaaatcggcc ataaagactt tcacattggc gttcagtgcg
     6841 ttgatcacca tcttgcgctc taccgggcca gttatctcta cgcggcggtc ttctaagtcc
     6901 gcaggaatcc cgcgaatttt ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa
     6961 tcaggcaacg ttccgttatc aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta
     7021 ttgcgttgtg gcgtaaaatg cgtcaccagc tcagtcagaa attctaccgc ttcggcagta
     7081 agaatttgct tctcctgctc gccatacggc cttgtgaaag ccagttcatc ggttgttgtt
     7141 gcctgttcag tcatcgtgca gctcctcgtc atggatccga aaacttcccc tctgtgaacg
     7201 aaggaacgtt gtgcgctttt cgttcagcac aactaagact actcatttaa aatgcaaaat
     7261 caaaaacaat ttccatttta attaaaaata cttgataact tattgataac aatatgaata
     7321 aaatttattc acttgctgag gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga
     7381 ggtaaggtgc tgaatcgctt aacgatcgac tatcacagaa gattaatcca gcgttggatt
     7441 catgtgccgt agatcgtatg gcgtgatctg atagacgtaa tagttgagcc agttggtaaa
     7501 cagcaaatta ccgtgactac gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg
     7561 gaaatagtta tacggtacat ccgggtctag tccggcttcc acatcgcgta aatattcctg
     7621 cgccagcgtt tgcgcatcat attcgggatg gcccgtcaca aaggcaatgc gcttatcttt
     7681 gctggcaaac agatatgcat ccccttcttc cgtctctgcc agaatttcca gatcggtgta
     7741 atcacgaatc aacgctgccg gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc
     7801 atcaaagcca cgcgtcagaa gcgcatgagg atggagaata tgatgctcgt aaacgccaga
     7861 gagtttgtcg gtgcgagttt gcttaggaat gccgtagagg atattgagcg cggcctgtac
     7921 cgcccagcag acaaacagcg tcgaggtgac gtgatctttc gaccactcca gcacctgttt
     7981 gatctgcggc cagtaagcga catcattaaa ctccaccagg cccagcggcg caccggttac
     8041 aatcagaccg tcaaagttct gttcctgaat atcttcaaag ttacagtaga agttgttcag
     8101 atgctctgcg ggcgtgttgc gcgattcacg ggaatcgatg cgcaacagct gaatatcgac
     8161 ctgcaaaggt gagtttgaaa gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg
     8221 catcaggtta aggatcagaa ccttaagtgg acgaatttcc tgaccagacg cacgagaagt
     8281 tgtcatcaca aagacgtttt cttcacgcaa gaaattgacg gcgggtagct cgtccggcac
     8341 acgaatcggc ataacctgat tacctcacta catacgctta tacgtttaga catccagata
     8401 gctgaagata accagaaaat ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt
     8461 caaggaaaag gctacgttag aatataagaa tgacgaaaag gagagaaaat ggttattagt
     8521 attcgccgct cacggcatga ggaaggggag gaactcgttg cgatttggtg tcgatctgtc
     8581 gatgccaccc acgatttttt atcagcagag tatcgggccg agctggaaga gctggtgcgt
     8641 tcctttctgc cggaagcacc gttgtgggtc gcggttaatg aacgggagca gccggttggg
     8701 tttatgttgt taagtgggca gcatatggat gcgctgttta tcgatcctga tgtgtgcggc
     8761 tgcggcgtgg gtcggatgct ggtggagcat gcgctctcga tggcaccgga actgacaacc
     8821 aacgttaatg agcaaaatga gcaggcggtt gggttctata agaaggtggg ttttaaggtt
     8881 acgggacgct ctgaggtgga cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat
     8941 gtgggggaat aagccgcccc tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DAEEGA010000129         8898 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEEGA010000129 DAEEGA010000000
VERSION     DAEEGA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.129"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            1..2887
                     /locus_tag="KLH46_003636"
                     /pseudo
     CDS             1..2887
                     /locus_tag="KLH46_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="KLH46_003637"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="KLH46_003637"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBE0380176.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="KLH46_003638"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="KLH46_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="KLH46_003639"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="KLH46_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8041)
                     /locus_tag="KLH46_003640"
     CDS             complement(7280..8041)
                     /locus_tag="KLH46_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HBE0380177.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="KLH46_003641"
     CDS             complement(8038..8697)
                     /locus_tag="KLH46_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBE0380178.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2174 a         2357 c         2319 g         2048 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat aagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccaacaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt atcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtagaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt cttttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgga gtgacataat ttcaggagtg
     8881 agggttaggg agaggttt
//
LOCUS       DAEEGA010000130         8770 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEEGA010000130 DAEEGA010000000
VERSION     DAEEGA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.130"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(79..870)
                     /gene="aadA1"
                     /locus_tag="KLH46_003642"
     CDS             complement(79..870)
                     /gene="aadA1"
                     /locus_tag="KLH46_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBE0380179.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(983..1813)
                     /locus_tag="KLH46_003643"
     CDS             complement(983..1813)
                     /locus_tag="KLH46_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HBE0380180.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2023..3036
                     /gene="intI1"
                     /locus_tag="KLH46_003644"
     CDS             2023..3036
                     /gene="intI1"
                     /locus_tag="KLH46_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBE0380181.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3239..3589
                     /locus_tag="KLH46_003645"
     CDS             3239..3589
                     /locus_tag="KLH46_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBE0380182.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3715..4275
                     /locus_tag="KLH46_003646"
     CDS             3715..4275
                     /locus_tag="KLH46_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE0380183.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            4278..7244
                     /locus_tag="KLH46_003647"
     CDS             4278..7244
                     /locus_tag="KLH46_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HBE0380184.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     VIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            7311..7688
                     /locus_tag="KLH46_003648"
     CDS             7311..7688
                     /locus_tag="KLH46_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0380185.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7889..8548)
                     /locus_tag="KLH46_003649"
     CDS             complement(7889..8548)
                     /locus_tag="KLH46_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HBE0380186.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1949 a         2479 c         2349 g         1993 t
ORIGIN      
        1 aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
       61 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
      121 aacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
      181 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
      241 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
      301 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
      361 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
      421 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
      481 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
      541 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
      601 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
      661 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
      721 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
      781 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
      841 cgatacttcg gcgatcacca cttccctcat gatgtttaac gtttgaaata aggggcgccg
      901 agcgccagcg aggggagcca aaagcttgct tttggccgtc ccgacttgat tgaagggttg
      961 ggcgattttg ccattagatt ttttataaat ttagtgtgtt tagaatggtg atcgcatttt
     1021 tcttggcttt tatgcttgat gttaaattcg accccaagtt tcctgtaagt gcggacacaa
     1081 aaacatattt atgtcctgat ttgcttataa taaacccttc aaaccatccg ttttgtaagg
     1141 ttctatttgc tgtgaatcct gcaccagttt tcccatacag ttttgtacta ttatccagat
     1201 cttgtagata catgttctct atggtgtttt ctatggctga gtttttaact gggagattgt
     1261 gattaataat tttacgcagg aattgaattt gttcttctgg tgaaattttt aagctacttt
     1321 cgagccatgc ttctgttaat ccgttgtttc tttctttatc tccagagaag tcttgatttc
     1381 cataatcaaa atctttgaga taattcttga ttttatttaa tccaattttt tgggttattt
     1441 cttgcgaaac ccaaacaaca gaaaattgca tccacgtctt tggtgtatga ttgctgttcc
     1501 agatctccat tcctttgggg gttttatccc atttgaatat ggttttctga tctattattt
     1561 ccgcatcaaa tgccataagt gataatgcga tcttgaaagt tgaatctggt gccatttgcg
     1621 ttgcacactt tgctttattg aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta
     1681 aaaaacaacc ttcagttcct tcaaataatg gagatgcaac agtagagata tctgttgatg
     1741 cactggcgct gctgtagata atatttgcaa ttattaaaaa aatagcgaag ttgatatgta
     1801 ttgtgttttt cataataagt attggtttgg taaagggctt aattttaacg gctaacaatt
     1861 aatgaggctc cgggttcgcc caactttgtt ttagggcgac tgccctgctg cgtaacatcg
     1921 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     1981 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     2041 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     2101 ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc cttcatccgt
     2161 ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc
     2221 tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg
     2281 ctgttcttct acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga
     2341 cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc
     2401 ggttttctgg aaggcgagca tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg
     2461 atcagtgagg gtttgcaact gcgggtcaag gatctggatt tcgatcacgg cacgatcatc
     2521 gtgcgggagg gcaagggctc caaggatcgg gccttgatgt tacccgagag cttggcaccc
     2581 agcctgcgcg agcagctgtc gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc
     2641 cgcagcggcg ttgcgcttcc cgacgccctt gagcggaagt atccgcgcgc cgggcattcc
     2701 tggccgtggt tctgggtttt tgcgcagcac acgcattcga ccgatccacg gagcggtgtc
     2761 gtgcgtcgcc atcacatgta tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa
     2821 caagcaggca tcacgaagcc cgccacaccg cacaccctcc gccactcgtt cgcgacggcc
     2881 ttgctccgca gcggttacga cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc
     2941 tctacgacga tgatttacac gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg
     3001 cttgatgcgc tgccgcccct cactagtgag aggtagggca gcgcaagtca atcctggcgg
     3061 attcactacc cctgcgcgaa ggccatcggt gccgcatcga acggccggtt gcggaaagtc
     3121 ctccctgcgt ccgctgatgg ccggcagcag cccgtcgttg cctgatggat ccaacccctc
     3181 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacaat
     3241 ggaggtggta gccgagggtg tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg
     3301 ttgcgacacg gtgcagggtt tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg
     3361 cttcgtcaac caatggagga acaccaccat gaacgccaat gaaccgagca ccagttgctg
     3421 cgtgtgctgc aaggaaatcc cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt
     3481 ggagcatttc tgcgggctgg agtgctatca gcgcttccag gcgcgggcca gcactgcgac
     3541 cgaaaccagc gtcaaaccgg acgcttgtga ttcgccgccg tcaggttgag gcatacccta
     3601 acctgatgtc agatgccatg tgtaaattgc gtcaggatag gattgaattt tgaatttatt
     3661 gacatatctc gttgaaggtc atagagtctt ccctgacatt ttgcagggaa ttccatgact
     3721 ggacagcgca ttgggtatat cagggtcagc accttcgacc agaacccgga acggcaactg
     3781 gaaggcgtca aggttgatcg cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt
     3841 ccgcaactgg aagcgctgat aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc
     3901 atggatcgcc tggcgcgcaa tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa
     3961 cgcggcgtgc atatcgaatt cgtcaaggaa cacctcagtt ttactggcga agactctccg
     4021 atggcgaacc tgatgctctc ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc
     4081 cgcgagcgtc agcgcgaggg tattgcgctc gccaagcaac gcggggctta ccgtggcagg
     4141 aagaaatccc tgtcgtctga gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag
     4201 caaaagacca agcttgctcg tgaattcgga atcagtcgcg aaaccctgta tcaatacttg
     4261 agaacggatc agtaaatatg ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc
     4321 tgctggcgtt gccggactcc aaggacgacc tgatccgaca ttacacattc aacgataccg
     4381 acctctcgat catccgacag cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc
     4441 tctgttacct gcgctttccc ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct
     4501 tgttgaagct ggtcgccgac cagctcaagg tcggcgtcga aagctggaac gagtacggcc
     4561 agcgggagca gacccggcgc gagcacctga gcgagctgca aaccgtgttc ggtttccggc
     4621 ccttcaccat gagccattac cggcaggccg tccagatgct gaccgagctg gcgatgcaaa
     4681 ccgacaaagg catcgtgctg gccagcgcct tgatcgggca cctgcggcgg cagtcggtca
     4741 ttctgcccgc cctcaacgcc gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc
     4801 ggcgcatcta cgacgccttg gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg
     4861 atctgctcaa gcgccgggac aacggcaaga cgacctggtt ggcttggttg cgccagtctc
     4921 cggccaagcc aaattcgcgg catatgctgg aacacatcga acgcctcaag gcatggcagg
     4981 cactcgatct gcctaccggc atcgagcggc tggttcacca gaaccgcctg ctcaagattg
     5041 cccgcgaggg cggccagatg acacccgccg acctggccaa attcgagccg caacggcgct
     5101 acgccactct cgtggcgctg gccaccgagg gcatggccac cgtcaccgac gaaatcatcg
     5161 acctgcacga ccgcatcctg ggtaagctgt ttaacgctgc caagaataag catcagcagc
     5221 agttccaggc gtcaggcaag gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc
     5281 aggcgctgat cgacgccaag caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca
     5341 tgtcctggga ttcctttgcc gagagcgtca ccgaggcgca gaagctcgcg caacccgatg
     5401 acttcgattt cctgcatcgc atcggcgaga gctacgccac cctgcgccgc tatgcaccgg
     5461 aattccttgc cgtgctcaag ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca
     5521 ttgaggtgct gcgcggcatg aacaccgaca acgcccgcaa gctgccagcc gatgcaccga
     5581 ccggcttcat caagccgcgc tggcagaaac tggtgatgac cgacgccggc atcgaccggc
     5641 gctactacga actgtgcgcg ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct
     5701 gggtgcaggg ttcacgccag ttcaaggact tcgaggacta cctggtaccg cccgagaagt
     5761 tcaccagcct caagcagtcc agcgaattgc cgctggccgt ggccaccgac tgcgaacaat
     5821 atctgcatga gcggctgacg ctgctggaag cacaacttgc caccgtcaac cgcatggcgg
     5881 cagccaacga cctgccggat gccatcatca ccgagtcggg cttgaagatc acgccgctgg
     5941 atgcggcggt gcccgacacc gcgcaggcgc tgatagacca gacagccatg gtcctgccgc
     6001 acgtcaagat caccgaactg ctgctcgaag tcgatgagtg gacgggcttc acccggcact
     6061 tcacgcactt gaaatcgggc gatctggcca aggacaagaa cctgttgttg accacgatcc
     6121 tggccgacgc gatcaacctg ggcctgacca agatggccga gtcctgcccc ggcacgacct
     6181 acgcgaagct cgcttggctg caagcctggc atacccgcga cgaaacgtac tcgacagcgt
     6241 tggctgaact ggtcaacgct cagtttcggc atccctttgc cgggcactgg ggcgatggca
     6301 ccacatcatc atcggacgga cagaatttcc gaaccgctag caaggcaaag agcacggggc
     6361 acatcaaccc aaaatatggc agcagcccag gacggacttt ctacacccac atctccgacc
     6421 aatacgcgcc attccacacc aaggtggtca atgtcggcct gcgcgactca acctacgtgc
     6481 tcgacggcct gctgtaccac gaatccgacc tgcggatcga ggagcactac accgacacgg
     6541 cgggcttcac cgatcacgtc ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc
     6601 gcatccgcga cctgggcgac accaagctct acatcccgaa gggcgatgcc gcctatgacg
     6661 cgctcaagcc ggtgatcggc ggcacgctca acatcaagca cgtccgcgcc cattgggacg
     6721 aaatcctgcg gctggccacc tcgatcaagc agggcacggt gacggcctcg ctgatgctca
     6781 ggaaactcgg cagctacccg cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc
     6841 gcatcgagcg cacgctgttc atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg
     6901 tgcatgccgg gctgaacaag ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca
     6961 accgccttgg tgaaatccgt gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc
     7021 tcaacctggt gacggcggcc atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc
     7081 atgcgttgcg cggcaatggt catgccgtcg atgactcgct attgcagtac ctgtcgccac
     7141 tcggctggga gcacatcaac ctgaccggtg attacctatg gcgcagcagc gccaagatcg
     7201 gcgcggggaa gttcaggccg ctacggcctc tgcaaccggc ttagcgtgct ttattttccg
     7261 ttttctgaga cgacccctat tatggcgaag gtatcattca ggaagcgttg atgaaggatt
     7321 atctctgtaa aaagttattc aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg
     7381 gcaataacat tactggcctg gcatgttgca atattcttta tccctcgccc cgatacagcg
     7441 gtcagctgca tattaaagag ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta
     7501 aagcgataat gcgctttata gcccggcttg cgcttgaaca ggaatgcctt agccttagct
     7561 ggaacgctga aaaatccaac cccggcgcta accgttttta tcaggctctg ggaggcagaa
     7621 taaatgatca tatcgtcaat tattacctcc acggggagag cctgagcaaa ctggcctcag
     7681 gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa
     7741 tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc gaatttgctt
     7801 tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcaa ccaggcgttt
     7861 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact
     7921 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     7981 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     8041 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     8101 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     8161 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     8221 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     8281 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat
     8341 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     8401 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     8461 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     8521 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     8581 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     8641 cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
     8701 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc
     8761 aaagtgcgtc
//
LOCUS       DAEEGA010000131         8643 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEEGA010000131 DAEEGA010000000
VERSION     DAEEGA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8643
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.131"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(768..1964)
                     /gene="hemY"
                     /locus_tag="KLH46_003650"
     CDS             complement(768..1964)
                     /gene="hemY"
                     /locus_tag="KLH46_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBE0380187.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1967..3178)
                     /gene="hemX"
                     /locus_tag="KLH46_003651"
     CDS             complement(1967..3178)
                     /gene="hemX"
                     /locus_tag="KLH46_003651"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBE0380188.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(3200..3940)
                     /gene="hemD"
                     /locus_tag="KLH46_003652"
     CDS             complement(3200..3940)
                     /gene="hemD"
                     /locus_tag="KLH46_003652"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBE0380189.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3937..4878)
                     /gene="hemC"
                     /locus_tag="KLH46_003653"
     CDS             complement(3937..4878)
                     /gene="hemC"
                     /locus_tag="KLH46_003653"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBE0380190.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5265..7811
                     /gene="cyaA"
                     /locus_tag="KLH46_003654"
     CDS             5265..7811
                     /gene="cyaA"
                     /locus_tag="KLH46_003654"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBE0380191.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7851..8171)
                     /gene="cyaY"
                     /locus_tag="KLH46_003655"
     CDS             complement(7851..8171)
                     /gene="cyaY"
                     /locus_tag="KLH46_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBE0380192.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8219..>8643)
                     /locus_tag="KLH46_003656"
     CDS             complement(8219..>8643)
                     /locus_tag="KLH46_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBE0380193.1"
                     /translation="QFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRS
                     FNSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPA
                     KTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1966 a         2234 c         2223 g         2220 t
ORIGIN      
        1 gttgacgggg tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca
       61 agacgtttgg gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat
      121 gatgacgtaa ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa
      181 taatagtgac aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag
      241 cgttttcaat cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg
      301 attattgatg ctgtttttag ttttaacggc aattaatata tgtgttatta attgaatgaa
      361 ttttatcatt cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa
      421 gttattactc aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg
      481 ttatttcccg attctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt
      541 cgccttagtg cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca
      601 ctgacagatg tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc
      661 acttgttgtc atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt
      721 atgcatcaga aaggtcccgg aggtgctttc ctccgggtga gaaggaacta ctgtggcggg
      781 ttattctgca acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg
      841 tgcagtctgt ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc
      901 agcgccgcgc ggaaggcgag cgatgcttcc tgccattcac cgtgcttcat cagtgactga
      961 cccaatgtgc tccacaacag cgggcgatca ccgacgtttt tgatttgctg gcgtagcact
     1021 ttttccagct gttccgggtt attggttttc agacgcggga tcggcagcag taggcgatcg
     1081 tcatactggc gtttcaggcc gtcgatgata atttgctggg cagtatcatg atcgtcacat
     1141 tcaataagat gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt
     1201 tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc
     1261 aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct
     1321 ttcgccattg atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc
     1381 tgttccgcca gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatccacg
     1441 ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct
     1501 accggaatgg tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt
     1561 gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt
     1621 tcagcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc
     1681 agtttcagca gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac
     1741 catccacggg tgtgcgcgcc agtgcggaag atccgccgca gtagccactc aatggcaaac
     1801 agcactacca tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg
     1861 tagttgtcgg tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact
     1921 atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg
     1981 gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg
     2041 caggtgcagg tttagcttcc gttgtccccg ctgccggttg tgccagcagg ttacgcacgc
     2101 gagtctgcat cagtttttcc agcatcgcct ggctttgcag ggtttccgga agatccatcg
     2161 agatattttg ctggcttaac tggtccacct cgtcgaggaa cgctttggtg gtggcatcat
     2221 cagtatcgta gtaagcacgt acccaggtgg agacgttctc cagcgcctgg cgataagtct
     2281 cttcctgatg acgcggtaca gcttgtgctg cgaccagcag gcgagagcga atattttcgc
     2341 gcagatagat atcctgattt ggcgctaaca gcggtacggc ggtgtcatca cgacggcgaa
     2401 tcgtaatgaa gttgtccata aagttctgcc agcttttttg cagattgata cgccattcgc
     2461 tgatggaact ggaaagctct tcaccgtctg aatccatcgg cgaaccatcg ctgtcattat
     2521 cggccagacg caggttatct acctgatttg aaagctgatt aagcttaagg atgatgccgt
     2581 cataatccac ctgcgatact gcagaaaggc tggcgatatc atcggtaatt gcccgacgaa
     2641 cggtaatcag actcgggtca ttcatatccg ccaggctggc gtctgcactt ttcagcaacg
     2701 ctgcagaggt cgtgacgtcc tgatcgctcc acagcttccg tccggcgagt ttcaccagaa
     2761 aatcggcctg agccagcagc caggttttag catcgctgcc ggaaatggtg gcgatctttt
     2821 gttggacttc atccaactgt tttgccagcg tttcttgctg acgattcgcc tgcttaagct
     2881 gcgcagcttg ttgcttaata atgccttcca gctcggcttt ttggctctcc tgggcttttt
     2941 gcaatgccgt cagttggtta gccagggcat cgctggtggc ggtctgattg acggcctgtt
     3001 gtttacccca gccatacaaa ccgacacccg ccgccagagc aatagcgata gccaccgcgc
     3061 tgagaatcaa tgcagtattg ttcttactct ttttttctgt tgcgacaggt tgtgacgtgg
     3121 tgtccacggc ctccctggtc tcttcaacca cggcggaggt tttttcttgt tccgtcatta
     3181 tggcttcctg ttatgagagt tattgtaatg cccgtaaaag cgcatcgttg tcagcgttat
     3241 cggcgacctt aatgtcttgc cagcccagtt cccgggcgag tttcgccaaa cgctcactga
     3301 cgaccaatag tcgacagtgt agtaaccagt gctcacgata ccattgtggg atcagcgacc
     3361 agagttgctg caacatttca ccgctggtaa caacgaccat cgtcacctcg cgggattgcc
     3421 agcgcatcgc ttcttctgca ccatcgtaat ggattgcgca tcgttgataa cattcacaaa
     3481 aagtgacctc agcaccgcgc accgtcaggg tatccccaat tagctcacga ccgccattgc
     3541 cacgtaatat cagcgcacgt ttgcccgcaa tattttgtaa ttcaggtaat tgtagcaaga
     3601 cttcgctgat ttcccgatcc tgtgggtaga gaatcttctg tccgcttacg gtatgtagcg
     3661 ccagtgcggt ggtgcgtcca atggcgaaat aagtaggtag ttggggccat ttacgatctt
     3721 gctgatgcag ctgtgattgg gcaaaagcaa ccgcgtgttg cgagagggca aacaacagat
     3781 cgctctcccc cagcgctgcc agttgatcag caagttgcgg taattgtcga cccggagaaa
     3841 actcaatcag cggaaaatgc caggccacct gccccagtgt gcgcagacgg ctcactaact
     3901 cttctccagc gggagacggg cgggtgacca ggatactcat gccggggcct ctccgttata
     3961 gacttcagcg aggatctcgc gcgcgccgtt attcagtagc tcttctgcca gcgaaatccc
     4021 catttgttcg gcatcttgcg gcgcaccgcg gcgttcaccg cgaataatct gcgaaccgtc
     4081 cggcgcgccg accagcgcac atagccagat ttcgccatca ataagctcgg cgtagctacc
     4141 aattggcacc tgacatccgc cttcgagacg ggtattcatg gcgcgttctg cggtaacgcg
     4201 cagtgcagtt tcgtggtgat tcagcgcggc aagcagctcg cgagtgcgtg tatcatcaag
     4261 gcggcattca ataccgactg caccttgtcc taccgccgga agagaaatct cgggtggtaa
     4321 cgcggcgcga atgcgggact ccagacctaa acgttttagt ccggctacgg caagaatgat
     4381 ggcatcgtat tcgccgttat ctagtttgct caggcgagtg ccgacgttgc cgcgcaggga
     4441 gcggataatc agatccgggc ggcgttcagc cagttggcac tggcgacgta aactggacgt
     4501 cccgacgata ctgcctgctg gtaacgcatc cagactgtca tagttattgg acacaaaggc
     4561 atcgcgagga tcttcacgct cgcaaatagt gaccagcccc agaccttgcg ggaattcaac
     4621 cggcacatct ttcattgaat gtacggcgat atcagcgcga ttctccagga gcgcgacttc
     4681 cagctctttt acaaataagc cttttccgcc tactttcgcc agcggcgtat caagaatcac
     4741 atcgccgcgc gtcaccatcg gtaccagttc aacgaccagg cccggatggc tcgccatcaa
     4801 cttgtctttg acatagtgtg cctgccagag tgcaagtggg ctttggcgtg tggcaattct
     4861 taaaacattg tctaacatgc ttgttaccgt cattatcatc cgtggtccat cctaacatcc
     4921 ttgccagagt gatgtcagtg ttgtggtgaa acgtagacgc ctgcgcaaac cgtaaaatga
     4981 ggtctggcag tggatcctga caggcgtttc acgccgtcgt aataaggaat ttacagagaa
     5041 taaacggtgc tacacttgta tgtagcgcat ctttctttac ggtcaatcag caaggtgtta
     5101 aattgatcac gttttagacc attttttcgt cgtgaaacta aaaaaaccag gcgcgaaaag
     5161 tggtaacggt tacctttgac atacgaaata tcccgaatgc cgcgtgttac cgttgatgtt
     5221 ggcggaatca cagtcatgac gggtagcaaa tcaggcgata cgtcttgtac ctctatattg
     5281 agactctgaa acagagactg gatgccataa atcaattgcg tgtggatcgc gcgcttgctg
     5341 ctatggggcc tgcattccaa caggtctaca gtctactgcc gacattgttg cactatcacc
     5401 atccgctaat gccgggttac cttgatggta acgttcccaa aggcatttgc ctttacacgc
     5461 ctgatgaaac tcaacgccac tacctgaacg agcttgaact gtatcgtgga atgtcagtac
     5521 aggatccacc gaaaggtgag cttccaatta ctggtgtata caccatgggc agcacctcgt
     5581 ccgtagggca aagttgttcc tctgacctgg atatctgggt ctgtcatcaa tcctggctcg
     5641 atagcgaaga gcgccagtta ctacaacgta aatgcagcct gctggaaagc tgggccgcct
     5701 cgctgggtgt ggaagtcagc ttcttcctga ttgatgaaaa ccgcttccgt cataatgaaa
     5761 gcggcagcct ggggggcgaa gattgtggct ccacccagca tatactgctg cttgacgaat
     5821 tttatcgtac tgccgtgcgt ctcgccggta agcgtattct gtggaatatg gtgccgtgcg
     5881 acgaagaaga gcattacgac gactatgtga tgacgcttta cgcgcagggc gtgctgacgc
     5941 caaatgaatg gctggatctc ggtggcttaa gctcgctttc tgctgaagag tactttggtg
     6001 ccagcctttg gcagctctac aagagtatcg attccccata caaagcggtg ctgaaaacac
     6061 tgctgctgga agcctattcc tgggaatacc cgaacccacg tctgctggcg aaagatatca
     6121 aacagcgttt gcacgacggc gagattgtat cgtttggcct cgatccatac tgcatgatgc
     6181 tggagcgtgt tactgaatac ctgacggcga ttgaagattt cacccgtctg gatttagtac
     6241 gtcgctgctt ctatttaaaa gtgtgcgaaa aactcagccg tgaacgcgcc tgtgtaggct
     6301 ggcgtcgcgc agtgttgagc cagttagtga gcgagtgggg ttgggacgaa gctcgtctgg
     6361 caatgctcga taaccgtgct aactggaaga ttgatcaggt gcgtgaggcg cacaacgagc
     6421 tgctcgacgc gatgatgcag agctaccgta atctgatccg ctttgcgcgt cgcaataacc
     6481 ttagcgtctc cgccagtccg caggatatcg gcgtactgac gcgtaaactg tatgccgcgt
     6541 ttgaagcatt gccaggtaaa gtgacgctgg taaacccgca gatttcaccc gatctctcgg
     6601 aaccgaatct gacctttatt tatgtgccgc cgggccgggc taaccgttca ggttggtatc
     6661 tgtataaccg cgcgccaaat attgagtcga tcatcagcca tcagccgctg gaatataacc
     6721 gttacctgaa taaactggtg gcgtgggcat ggtttaacgg cctgctgacc tcgcgcaccc
     6781 gtttgtatat taaaggtaac ggcattgtcg atttgcctaa gttgcaggag atggtcgccg
     6841 acgtgtcgca ccatttcccg ctgcgcttac ctgcaccgac accgaaggcg ctctacagcc
     6901 cgtgtgagat tcgccatctg gcgattatcg ttaacctgga atatgacccg acagctgcgt
     6961 tccgcaatca ggtggtgcat tttgatttcc gtaagctgga tgtcttcagc tttggcgaga
     7021 atcaaaattg cctggtaggt agcgttgacc tgctgtaccg caactcgtgg aacgaagtgc
     7081 gtacgctgca cttcaacggc gagcaatcga tgatcgaagc cctgaaaact attctcggca
     7141 aaatgcatca ggacgccgca ccgccagata gcgtggaagt cttctgttat agccagcatc
     7201 tgcgcggctt aatccgtact cgcgtgcagc aactggtttc tgagtgtatt gaactgcgcc
     7261 tttccagcac ccgccaggaa accgggcgtt tcaaggcgct gcgcgtttct ggtcaaacct
     7321 ggggcttgtt cttcgaacgc ctgaatgtat cggtacagaa gctggagaac gccatcgagt
     7381 tttatggcgc tatttcgcat aacaaactgc acggcttgtc agtgcaggtt gaaaccaatc
     7441 acgtcaaatt accggcggtg gtggacggct ttgccagcga agggatcatc cagttctttt
     7501 tcgaagaaac gcaagacgag aatggcttta atatctacat tctcgacgaa agcaaccggg
     7561 ttgaggtgta tcaccactgc gaaggcagca aagaggagct ggtacgtgac gtcagtcgct
     7621 tctactcgtc atcgcacgac cgcttcacct acggctcaag cttcatcaac ttcaacctgc
     7681 cgcagttcta tcagattgtg aaggttgatg gtcgcgaaca ggtgattccg ttccgaacaa
     7741 aatcgattgg caacctgccg cccgccaatc aggatcacga tacgccgcta ttacagcagt
     7801 atttttcgtg atgagcgtgc cggaaagcga ggcttatccg gcatgcaatc ttagcggaaa
     7861 ctgactgttt cacccgcctg ttgcgtcgcc gcctgttcca gcaaatccca gaaggtttcg
     7921 ccgctgcgat cgcaaatcca ttcatcgcct ttcaggtcaa aatggtagcc gccctgtttg
     7981 gttgccagcc acacctggtg cagcggctcc tggcggttaa taatgatttt gctgccgttt
     8041 tcaaaggtaa tggtcagtac gccgccgttg atttcgcagt cgatatcgct gtcgccatcc
     8101 ctgtcgtcca gacgctcttc aatggtcagc cacagttgat cagccaggcg atgaaattca
     8161 ctgtcgttca ttgttgtatc ctgtttttaa gtgatggcgg cagtatagcg gcatggggtc
     8221 agggcttcaa agtttgcacc tctgctgctg cgttccggca tgattcatcc gtcaccggaa
     8281 taatgatgtc tctgttttga acttcacgta cgttctcctc tctgacacga gttttcgccg
     8341 ggcaggggga gggcgtaatc cttacctctg tatcggtcca tgtataaaca ttgcgatcgt
     8401 cataagcgaa gcgatcgttt ttagttagcg aaccgttaac tggaataatt tgcaagctct
     8461 ttgaccgtaa ttggcggata aagtaagcat gctgtttgcc gctattaaat gagcgatcaa
     8521 tgaccaggtc tggcgtatcc agcgggatta tctcaagctg gtcttcacca aatgtcgata
     8581 attttgttac atacagccga tctttatatt gcgcaatatt gccagtaatc agatggaatt
     8641 gtt
//
LOCUS       DAEEGA010000132         8416 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEEGA010000132 DAEEGA010000000
VERSION     DAEEGA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.132"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            129..1826
                     /gene="maeA"
                     /locus_tag="KLH46_003657"
     CDS             129..1826
                     /gene="maeA"
                     /locus_tag="KLH46_003657"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBE0380194.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQRLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1960..2970
                     /gene="adhP"
                     /locus_tag="KLH46_003658"
     CDS             1960..2970
                     /gene="adhP"
                     /locus_tag="KLH46_003658"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBE0380195.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3116..3400
                     /locus_tag="KLH46_003659"
     CDS             3116..3400
                     /locus_tag="KLH46_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBE0380196.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="KLH46_003660"
     CDS             complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="KLH46_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBE0380197.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4274..5158)
                     /gene="fdnH"
                     /locus_tag="KLH46_003661"
     CDS             complement(4274..5158)
                     /gene="fdnH"
                     /locus_tag="KLH46_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBE0380198.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="KLH46_003662"
     CDS             complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="KLH46_003662"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7631..7633),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBE0380199.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
BASE COUNT         2020 a         2253 c         2140 g         2003 t
ORIGIN      
        1 gacgattaaa caccattgcc tgcgcaatgg tgtttttatc tgctttatac ttggggacga
       61 cgccctggcg gtaaagcaaa gacgataaaa gcgtgccagg gatggatatt caaaaaagag
      121 tgagtgacat ggaaccaaaa acaaaaaaac agcgttcgct ttatatccct tacgctggcc
      181 ctgtactgct ggaatttccg ttgttgaata aaggcagtgc cttcagcatg gaagaacgcc
      241 gtaacttcaa cctgctgggg ttactgccgg aagtggtcga aaccatcgaa gaacaagcgg
      301 aacgagcatg gatccagtat cagggattca aaaccgaaat cgacaaacac atctacctgc
      361 gtaacatcca ggacaccaac gaaaccctct tctaccgtct ggtaaacaat catcttgatg
      421 agatgatgcc tgttatttat accccaaccg tcggcgcagc ctgtgagcgt ttttctgaga
      481 tctaccgccg ttcacgcggc gtgtttatct cttaccagaa ccgccacaat atggacgata
      541 ttctgcaaaa cgtgccgaac cataatatta aagtgattgt ggtgactgac ggtgaacgta
      601 ttctggggct tggtgaccag ggcatcggcg ggatgggcat tccgatcggt aaactgtcgc
      661 tctataccgc ctgtggcggc atcagcccgg cgtataccct tccggtggtg ctggatgtcg
      721 gaacgaacaa ccaacagcgg cttaacgatc cgctgtatat gggctggcgt aatccgcgta
      781 tcactgacga tgaatactat gaattcgttg atgaatttat ccaggctgtg aaacaacgct
      841 ggccggacgt gctgttgcag tttgaagact ttgctcaaaa aaatgcgatg ccgttactta
      901 accgctatcg caatgaaatt tgttctttta acgatgacat tcagggcact gcggcggtaa
      961 cagtcggcac actgatcgca gccagccgcg cggcaggtgg tcagttaagc gagaaaaaaa
     1021 tcgtcttcct tggcgcaggt tcagcgggat gcggcattgc cgaaatgatc atcgcccaga
     1081 cccagcgcga aggattaagc gaggaagcag cgcggcagaa agtctttatg gtcgatcgct
     1141 ttggcctgct gacggacaag atgccgaacc tgctgccttt ccagaccaaa ctggtgcaga
     1201 agcgcgaaaa cctcagtgac tgggataccg acagcgatgt gttgtcactg ctggatgtgg
     1261 tgcgcaatgt aaaaccagat attctgattg gcgtctcagg acagaccggg ctgtttacgg
     1321 aagagatcat ccgtgagatg cataaacact gtccgcgtcc gatcgtgatg ccgctgtcta
     1381 acccgacgtc tcgcgtggaa gccacaccgc aggacattat cgcctggacc gaaggtaacg
     1441 cgctggtcgc cactggcagc ccgtttaatc ctgtggtatg gaaagataaa atctacccta
     1501 tcgcccagtg taacaacgcc tttattttcc cgggcatcgg cctgggtgtt attgcttccg
     1561 gcgcgtcacg tatcaccgat gagatgctga tgtcggcaag tgaaacgctt gctcagtatt
     1621 cgccgctggt cctgaacggc gaaggtctgg tactaccgga actgaaagat attcagaaag
     1681 tctcccgcgc aattgcgttt gcggttggca aaatggcgca gcagcaaggc gtggcggtca
     1741 aaacgtctgc tgaagctttg caacaagcca ttgacgataa tttctggcaa gccgaatacc
     1801 gcgactaccg ccgtacctcc atctaagcct gcacccggta gtgaaggcta ccgggctatt
     1861 tccctctccc tttttcagat ctcatccata ctgggtagtg gcgaataaat ctcatttgcc
     1921 tcacctgcta tgcagaacat catccgaaaa ggaggaacta tgaagactgc agttgttacg
     1981 aaggatcatc atgttgacgt tacggataaa acactgcgct cactgaaaca tggcgaagcc
     2041 ctgctgaaaa tggagtgttg tggtgtatgt cataccgatc ttcatgttaa gaatggcgat
     2101 tttggtgaca aaaccggcgt aattctgggc catgaaggca tcggtgtggt ggcagaagtt
     2161 ggcccaggtg tcacctcatt aaaaccaggc gatcgtgcca gcgtggcgtg gttttacgaa
     2221 ggatgcggtc attgcgaata ctgtaacagt ggtaacgaaa cgctctgccg ttcagttaaa
     2281 aatgccggat acagcgttga tggcgggatg gcggaagagt gcatcgtggt cgccgattac
     2341 gcggtaaaag tgccagatgg tctggactcg gcggcgccca gcagcattac ctgtgcgggg
     2401 gtcaccacct acaaagccgt taaactgtca aaaattcgtc ccggtcagtg gattgctatc
     2461 tacggtcttg gcggtctggg taacctcgcc ctgcaatacg caaagaatgt ctttaacgcc
     2521 aaagtgatcg ccattgatgt caatgatgag cagttaaaac tggcaaccga aatggatgca
     2581 gatttagcga ttaactcacg caccgaagac gccgccaaaa ttgtgcagga gaaaaccggt
     2641 ggcgctcacg ctgcggtggt aacagcagta gctaaagctg cgtttaactc ggcagttgat
     2701 gctgtccgtg caggcggtcg tgttgtggct gtcggtctgc cgccggagtc tatgagcctg
     2761 gatatcccac gtcttgtgct ggatggcatt gaagtggtcg gttcgctggt cggcacgcgc
     2821 caggatctaa ccgaagcctt ccagtttgcc gccgaaggca aagtggtgcc gaaagtcgcc
     2881 ctgcgtccgt tagcggacat caacaccatc tttaccgaga tggaagaagg caaaatccgt
     2941 ggccgtatgg tgattgattt ccgtcactaa gaggtctttg ctgcgactcc catgttcggg
     3001 tcgcagcatc gcacactctc caacatgaaa tggctaaatg gattattgtt gcgtaggaag
     3061 gaacaataat gatagtgttc ccagagaagt aacgctactg agagtaaaga cactaatgaa
     3121 aatggccaat catccccgcc cgggggacat tattcaggaa tcactggacg aacttaatgt
     3181 cagcctgcgc gagtttgcca gagcaatgga aattgcgccc tcaacggcaa gccgattgct
     3241 gaccggaaaa gcagctttga cgccagaaat ggcaattaaa ctttccgtgg tgatcggcag
     3301 ttcgccgcaa atgtggctga atctgcaaaa cgcctggagt ctggcagagg cagaaaaaac
     3361 ggtggttgtg tcgcgattac gccgtctggt tacgcaataa aaaatggcac cttataaagc
     3421 gccatttgaa actgatgaca aacgcaacat tgcctgatgc gctacgctta tcaggcctac
     3481 acgttcactt caatctattg tatttgaagg gttttcatgc cggataaggc attcacgacg
     3541 catccgggat gaacaaagcc cacgttgtct acaatatgaa aatggcgcct tttacagcgc
     3601 cattttcagc ttaaagttca aaagagatta tatcccttct tcactctctt ttttcgcttc
     3661 tgccttctcg atttcacgat accagcgcgg atggtgtttc ttcgcccagc gacggctcac
     3721 cttcccttcg atcatccctt taatcgaccc tttcacccag aatgccatat acatatggat
     3781 caggatggcg tggatcagga tgatacccgc agccgcgtgg atcagcaggc tgtagcgaac
     3841 aacctgcatc gggaagtact gcgcaaagta cggacgccag ataatcaccc cggtcaccag
     3901 cagcacgaaa atcatgctca tgatcgacca gaacatcatc ttttgcccgg cgttgtactt
     3961 accaacatcc gccactttgt gctcattgcc ttttaatact tcgacaatgt tcaacagcca
     4021 cggaatatct ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag
     4081 tgcgacgaaa atcgcaatgc cgaagaaggg gtgcaaaatg cgtcccatct gcggcgtacc
     4141 gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac
     4201 caggaagaag caaatcacca cggtccagtg acaggcgcga tcaataaatt tggtgcgcac
     4261 aatcattttc gacttactca tgatgatcct cctcgtcatc gtccacttcc ttattcgggc
     4321 caataccgat gtagtggaaa atcaacccgg caaaagtggc aataaagcca gccgctgcca
     4381 gcggtttcaa cgcgcctttc cacaggctta ccgaggtgtc gatcttcgga tctttcggca
     4441 gaccgtgata cagctccggc tgatcggcgt gatgcagcac gtacataacg tgcgtaccac
     4501 cgaccccttc cgggttgtag acgccagcat gttcgtaacc acgcgctttc agtttcgcca
     4561 cgcgctgttc cgccagctcc agcatctcct tcttggtgcc gaagtggata gccccggtcg
     4621 gacaggtttt cacacaagcc ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc
     4681 atttatatac ccggttatcc tctttgttga ggcgcggaat attaaacgga cacccggcaa
     4741 tgcagtaacc acagccgatg cagttttccg actggaaatc gacaatcccg ttagcgtact
     4801 gaatgattgc accagcagac gggcacgcct tcaggcagcc cggatcttca cagtgcatac
     4861 agccgtcttt acggatcagc cactccagct tgccattctg ttcggtttcg ctaaagcgca
     4921 tcaccgtcca ggacttggcg ctcagatcgg cggggttatc gtaaaccccg acgcagtgcc
     4981 ccacttcatc acggatgtcg ttccactccg aacacgccac ctgacaggct ttacagccga
     5041 tacaggtgga aacgtcgata agtttcgcga cttctgcttt gtaatcacgc acctgagaag
     5101 gcggcgtgat ggagttagtt gcggaccttt tgataatatc ctgcgtttcc atagccatct
     5161 gttcgccccc ttacgccttc tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg
     5221 agtttgcatc accgacattc ggcgtcagag tgttagcgat ataaccttta cgcgcgacac
     5281 cctcaaagcc ccagtggatt ggaataccca ccgtttcaac ctgctgaccg tttacattca
     5341 gcggtttcag acggcgcgtt accactgcca ccgcgcggat aaagcctcgc ttgctggaga
     5401 cagtgacacg atcgccatta ttaatgcctt tcgccgccgc ctgcgtttcg ctgatttcca
     5461 caaactgttc cggctgagca attgcgttga gcaacgcgtg cttggtccag gtgtggaagt
     5521 gctcggtcag acgataggtc gtacccacat acgggaactg ctctttttta cccatccgca
     5581 gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac cacgttcgga tgcagcgggt
     5641 tagtgcccag cggcgtttca atcggctcgt agtgttccgg gaacggacct tccgccattt
     5701 tgttgatggc aaacaggcgt cccatccctt ccggctgcat aataaacggc ccggttggcg
     5761 tacccggtgc ggcattgccg aagtccggaa tatcgttacc cgtccacttg ctgccgttcc
     5821 actggatcag catccgtttc ggatcccacg gtttaccgtt gatatccgcc gaagcacggt
     5881 tgtagagtac gcgacggttg agcggccacg cccaggccca tcccagcgta ttccccagac
     5941 cggacgggtc ggagttatcg cggtttgcca tctggttacc ctgctctgtc cagctaccgg
     6001 tgtagatcca gcaagaagat gcggttgtac cgtcatcacg cagatgcgca aagctactca
     6061 gcaactgacc tttcttcgca atcagcacgc cattagcatc atagagatct tccaacgcat
     6121 agccgttgtt ctctttagct acttcgtcag attgcggttc gtgcggctgc ttgtagttcc
     6181 agctcatctt catcagcggt tctacgcctt taccaccttc ggcctggtac aactcgcgca
     6241 gatgatggta gatacccgcc agaatttcgc cgtcgttacg cgcttcgccc ggcgcgtcct
     6301 gacctttcca gtgccactgc aaccagcgac cggagttagc gatagaacca tcctcttcag
     6361 caaagcaggt cgaaggcaga cggaatactt cagtctgaat agacgccgga tcgacatcgt
     6421 tcgactcgcc gtggttctgc cagaaggtag aggtttcagt caccagcgga tcgataacca
     6481 ccatgtactt cagtttgctc aggcagctca ccactttgtt tttatccggg aaggacgcaa
     6541 ccgggttaaa gccctggcag aaataaccgg taactttgcc ttcatccatc atgttgaaat
     6601 acttgatgac gtcgtaggtc tggtcccact tcggcagcca gtcatagccc cagttgttct
     6661 ctttctgcgc ggcatcgcca tagaaagatt tcatcaggct aacgaagaac ttcggatagt
     6721 tgctccagta gttcacctga tcagccagcg tcgctttcgg cgtgttcgct tccagatacg
     6781 actgcaaatc aacctgtttt tctgacggca gcgtcagata acctggcagg ctggtagaga
     6841 gcagacctaa gtcagtcagc ccctgaatgt tggagtgacc acgcaatgcg ttcacgccgc
     6901 caccggccat acccatgtta ccgagcagca actggatcat cgccatagta cggatgttct
     6961 gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg
     7021 gtgcgctggt ggaggccagc acttcacaca ctttcaggaa gtcggctttt ggcgtaccac
     7081 agatgttttc aacaacgtcc ggcgtgtagc gggaaacgtg cgctttcagc aggttccaca
     7141 cacagcgcgg atgagtcagt gtttcatcgc gtttcgcata gccgttttca tcgaactgat
     7201 agttccagga cgatttgtcg tactggcgtt tttcagcgtc ataaccgctg aacaggccat
     7261 cttcgaaagc aaaatcatca cgcaccagta ggctggcgtt ggtgtaatgt ttaacgtatt
     7321 cggcgttgat tttgttgttt tcgatcaggt agcgcaaaat gccagacagg aacgtaatgt
     7381 ccgtaccgga acgaataggt gcgtaaatat ccgccacaga agcggtacgc gtaaaacggg
     7441 gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac
     7501 cgacgggatg cgcttcagca gcgttaccgc ccattaccat tacgacgtta gcgtttttga
     7561 tatccaccca gtggttggtc atagcaccgc gaccaaatgt tggagcaaga cttgctaccg
     7621 ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag catcccgagg gagcgggcaa
     7681 atttctgggt cagcatcccg gtttcgttgc tggcaccgga ggcacacagc ataccggtag
     7741 aaagccaacg gtttaccgtt acgccctgct cgttcttttc aataaagtta gcgtcacggt
     7801 cagctttcat cagcttcgca atacgggaga atgcttcttc ccagctaatg cgctgccatt
     7861 tgtcagaacc tggcgcacga tatttcgggt agcgcagacg gttttcactg ttgacgtaat
     7921 ccagcaaacc ggcccctttc gggcacagcg caccacggct taccggatga tccgggtcac
     7981 cttcaatgtg ataaatcgct tctctggcgt tttttgcgcc atcacccagg ctatacatca
     8041 atagcccgca acctacggaa cagtatgtgc aggtgttacg gatctcttta gcgcgtaata
     8101 atttgtagtt tcgcgcctga gccagtgctt gcttcggggc aaagcccaat gccgctaccg
     8161 ttgttccagc cataccgccc gcgcagattt taaaaaattg tctgcgactg acgtccattg
     8221 ctttcctctt ttttcagggg tattactgcg cgaggaaact aacagtaaaa ttctgaatct
     8281 ttttgcgcga aatcaaaaag aacacgtgtt tgccacaaaa taatccgcct gcgcaaattg
     8341 acctacctca atagcggtag aaaaacgcac cactgcctga caggccagtt aaaaaaatgc
     8401 tataaaattc agctta
//
LOCUS       DAEEGA010000133         8396 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEEGA010000133 DAEEGA010000000
VERSION     DAEEGA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.133"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            4..817
                     /gene="ttdA"
                     /locus_tag="KLH46_003663"
                     /pseudo
     CDS             4..817
                     /gene="ttdA"
                     /locus_tag="KLH46_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            814..1419
                     /gene="ttdB"
                     /locus_tag="KLH46_003664"
     CDS             814..1419
                     /gene="ttdB"
                     /locus_tag="KLH46_003664"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBE0380200.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1467..2930
                     /gene="ttdT"
                     /locus_tag="KLH46_003665"
     CDS             1467..2930
                     /gene="ttdT"
                     /locus_tag="KLH46_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBE0380201.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(2973..3986)
                     /gene="tsaD"
                     /locus_tag="KLH46_003666"
     CDS             complement(2973..3986)
                     /gene="tsaD"
                     /locus_tag="KLH46_003666"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBE0380202.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4224..4439
                     /gene="rpsU"
                     /locus_tag="KLH46_003667"
     CDS             4224..4439
                     /gene="rpsU"
                     /locus_tag="KLH46_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBE0380203.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4550..6295
                     /gene="dnaG"
                     /locus_tag="KLH46_003668"
     CDS             4550..6295
                     /gene="dnaG"
                     /locus_tag="KLH46_003668"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBE0380204.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6490..8331
                     /gene="rpoD"
                     /locus_tag="KLH46_003669"
     CDS             6490..8331
                     /gene="rpoD"
                     /locus_tag="KLH46_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBE0380205.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2027 a         2254 c         2253 g         1862 t
ORIGIN      
        1 gcagtgaata agttgacaga gattgtcgct aactttaccg ccatgatttc tacccgaatg
       61 cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc gtcgatgggg
      121 aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct gaatcgtcct
      181 gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg cttcccactg
      241 cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt caaagcgcca
      301 ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa taccggtagc
      361 ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga aatcgaagtt
      421 tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat gccgtcagaa
      481 ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc cgtaaacgcc
      541 tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc cgccgtactc
      601 tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa agcggcagaa
      661 ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca agggctgacc
      721 ggcaacagtt cagtgatggg cgcatcgtcg cggcacgctg ctggttcatg ccgatctcac
      781 ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa agatcctgac aaccccgatc
      841 aaagctgaag atctgcaaga tattcgcgtc ggcgatgtga tctacctgac cggtacgctg
      901 gtgacctgcc gcgacgtttg tcaccgccgt ttgatcgaac tgaaacgtcc gatcccttac
      961 gatctcaacg gcaaagcgat tttccacgct ggccccatcg tgcgcaaaaa cggtgacaaa
     1021 tgggagatgg tctccgtcgg cccgacaacc agtatgcgta tggaaagttt tgaacgtgaa
     1081 tttattgagc agaccggcgt aaaactggtg gttggcaaag gtggtatggg gccgctgacc
     1141 gaagaaggct gccagaaatt caaggcgtta catgtgattt tcccagcagg ctgcgcggtg
     1201 ctggcggcaa cccaggtaga agagattgaa gaagtgcact ggacagaact cgggatgccg
     1261 gagtcactgt gggtctgccg ggtcaaagag ttcggcccgc tgattgtctc tattgatacc
     1321 cacggcaaca acctgatagc cgaaaacaaa aagctgttcg ccgaacgccg cgatcccatc
     1381 gtggaagaga tctgcgagca cgtccattac atcaaataac cctcccggag aggctcaccc
     1441 ctctcctttt tcgcaggcat aacacgatga aaccttccac tgaatggtgg cgatatcttg
     1501 cgccgctggc ggtcatcgcc attattgctc tacttccggt tcccgcaggg ctggagaacc
     1561 atacctggct ctactttgcc gtttttactg gcgtgatcgt tggactgatc ctcgaacccg
     1621 tgccgggtgc cgtggtggcg atggtgggta tctccatcat cgccatactc tctccctggc
     1681 tgctgttcag ccccgaacag ctcgcccagc caggctttaa gttcaccgcc aaatccctct
     1741 cctgggcagt ttctggtttt tctaattcgg ttatctggct cattttcgcc gcctttatgt
     1801 ttggcacagg ctatgaaaaa accgggcttg gacgccgcat tgcgctgatt ctggtgaaaa
     1861 agatggggca tcgcacgctg tttctcggct atgcggtgat gttctccgag ctgatcctgg
     1921 cacctgtaac accgtccaac tcggcgcgtg gtgcgggtat tatctatccc atcatccgta
     1981 acctgccacc gctctatcaa tcacaaccaa acgacagcag ttcgcgcagc attggctcgt
     2041 acatcatgtg gatggggatt gttgccgact gcgtgaccag cgccattttc ctgacggcga
     2101 tggcacctaa cttgctgtta attggactga tgaaaagcgc atctcacgcc acgctgagtt
     2161 ggggcgactg gttcctcggg atgttgccgc tcagcatttt actggttctg ctggttccct
     2221 ggctggctta cgtgctgtac ccgccggtac tgaagtctgg tgatcaggtg ccgcgctggg
     2281 cagagacgga actgcaggca atgggcccgc tctgttcgcg tgaaaaacgg atgctggggc
     2341 tgatggtagg cgcgctggtg ctgtggattt tcggcggtga ttatatcgat gccgcgatgg
     2401 tcggttacag cgtggtggcg ctgatgctgc ttctgcgcat tatcagttgg gacgacattg
     2461 tcagtaataa agcggcatgg aacgttttct tctggctggc ctcgcttatc accctcgcga
     2521 ccggactcaa caataccggt tttattagct ggtttggcaa actgttagca ggcagcttaa
     2581 gtggttattc gccgacgatg gtgatggtga cgttgattgt ggtgttttat ctactgcgct
     2641 actttttcgc cagcgccacg gcgtatacct gcgccctcgc accgatgatg atcgccgccg
     2701 cgctggcgat gccggaaatc ccgctgccgg tgttctgcct gatggttggc gcggcaattg
     2761 gtctggggag cattcttaca ccatacgcca ccggacccag tccgatttac tacggtagtg
     2821 gttatctgcc aacggcggat tactggcgac tgggggcgat ttttgggctg atattcctcg
     2881 tgttgctggt gattaccggc ttactgtgga tgcccgtggt gttgctttaa gtgaggacgg
     2941 tgccggaaat tgctctccgg cacccaatag ttttacgcag ccggtaactc cgccagcggc
     3001 cagcgcggac gcacgctaac accgagatcc gccgtcgcgc ctgctttaaa ccgcaccatt
     3061 ccggcatagg cgatcatcgc gccgttatca gtacaaaatt ccggacgcgc gtagaacact
     3121 tcgccgcggc gttttttcat catttcagcc agcttcgccc gtaacgtgcg gttagcactc
     3181 acgccgcccg ccatgaccag tcgcttaaag cccgtctgat ccaacgctcg cttacactta
     3241 atcatcaacg tatcgaccac cgcatcttca aaggcgcggg cgatatcagc acgcgtctgg
     3301 tcgtcggtgc cgttgtcacg aatcgtgttc gccgcaaagg ttttcagacc agaaaagctg
     3361 aaatccagcc ccggacggtc ggtcatcgga cgcgggaaaa caaagcgccc ggcagtaccc
     3421 tgagccgcca ttttcgacag taacggtccg ccaggataat ccagccccag cagcttcgcg
     3481 gttttatcaa acgcttcacc ggcggcatca tcgatagact cgccgagcag ctcgtactga
     3541 ccaatgccag tcacgctgat taactgcgta tgaccgccgg acaccagcag cgcgacaaac
     3601 ggaaattccg gcgggttatc ttccagcatc ggcgctaaca gatgcccttc catatggtgt
     3661 accgggattg ccggaacgtt ccaggcaaac gccagagaac gccccacggt tgcgccaacc
     3721 agcagtgcgc cgactaatcc agggcctgcg gtataggcca cagcatcaat gtcttttgcc
     3781 gttaaaccag actcctttag cgccgcctgg atcaacggta cggttttacg cacatgatcg
     3841 cgggaggcca gttcaggcac gacgccgccg tagtcagcgt gcaatttcac ctgactatac
     3901 aattggttgg ctaacaaacc tttttcatcg tcgtaaatgg cgatgccggt ttcatcgcag
     3961 gaagtttcaa tacccagtac acgcatgact tgttttacct cgctttatta ccgcgcagtg
     4021 taggaccaat gcgggttgat gtaaaacttt gttcgcccct ggagaaagcc tcgtgtatac
     4081 tcctcaccct tataaaagtc cctttcaaaa aaggccgcgg tgctttacaa agcagcagca
     4141 attgcagtaa aattccgcac cattttgaaa taagctggcg ttgatgccag cggcaaaccg
     4201 aattaatcaa aggtgagagg cacatgccgg taattaaagt acgtgaaaac gagccgttcg
     4261 acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc aggtgttctg gcggaagttc
     4321 gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa gcgcgctaaa gcttctgcag
     4381 tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg ccgcactcgt ctgtactaat
     4441 tccccgagag cgttgctctc cgatcagacc gagttgtagt tgtaaggccg tgcttccgaa
     4501 aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa tcggggccta tggctggacg
     4561 aatcccacgc gtattcatta atgatctgct ggcacgcact gacatcgtcg atctgatcga
     4621 tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac gcgtgttgtc cattccacaa
     4681 cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag ttttaccact gctttggatg
     4741 tggcgcgcac ggcaacgcga tcgacttcct gatgaactac gacaagctcg agttcgtcga
     4801 aacggtcgaa gagctggcag caatgcacaa tcttgaagtg ccatttgaag caggcagcgg
     4861 ccccagccag atcgagcgcc atcagaggca aacgctttat cagttgatgg acggtctgaa
     4921 tacgttttac caacaatctt tacaacaacc tgttgccacg tctgcgcgcc agtatctgga
     4981 aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg attggttttg cgccccccgg
     5041 ctgggacaac gtcctgaagc ggtttggcgg caatccagaa aatcgccagt cattgattga
     5101 tgcggggatg ttggtcacta acgatcaggg acgcagttac gatcgtttcc gcgaacgggt
     5161 gatgttcccc attcgcgata aacgcggtcg ggtgattggt tttggcgggc gcgtgctggg
     5221 caacgatacc cccaaatacc tgaactcgcc ggaaacagac attttccata aaggccgcca
     5281 gctttacggt ctttatgaag cgcagcagga taacgctgaa cccaatcgtc tgcttgtggt
     5341 cgaaggctat atggacgtgg tggcgctggc gcaatacggc attaattacg ccgttgcgtc
     5401 gttaggtacg tcaaccaccg ccgatcacat acaactgttg ttccgcgcga ccaacaatgt
     5461 catttgctgt tatgacggcg accgtgcagg ccgcgatgcc gcatggcgag cgctggaaac
     5521 ggcgctgcct tacatgacag acggccgtca gctacgcttt atgtttttgc cggatggcga
     5581 agaccctgac acgctggtac gaaaagaagg taaagaagcg tttgaagcgc ggatggagca
     5641 ggcgatgcca ctctccgcat ttctgtttaa cagtctgatg ccgcaagttg atctgagtac
     5701 ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca ttgatatcgc aagtgccggg
     5761 cgaaacgctg cgaatatatc ttcgtcagga attaggcaac aaattaggca tacttgatga
     5821 cagccagctt gaacgattaa tgccaaaagc ggcagagagc ggcgtttctc gccctgttcc
     5881 gcagctaaaa cgcacgacca tgcgtatact tatagggttg ctggtgcaaa atccagaatt
     5941 agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat aagctccctg gacttggctt
     6001 attcagagaa ctggtcaaca cttgtctctc ccagccaggt ctgaccaccg ggcaactttt
     6061 agagcactat cgtggtacaa ataatgctgc cacccttgaa aaactgtcga tgtgggacga
     6121 tatagcagat aagaatattg ctgagcaaac cttcaccgac tcactcaacc atatgtttga
     6181 ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt gagcgcacgc atggtttaag
     6241 caacgaagaa cgcctggagc tctggacatt aaaccaggag ctggcgaaaa agtgatttaa
     6301 cggcttaagt gccgaagagc gatcgggaag cccccgacag ccgcactgag aggcagcggc
     6361 aaatatataa gtacgccctc gtaattatcg ttggcggtaa acaaccgttg gatttcagcg
     6421 ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa cctcatgaaa taagtgtgga
     6481 taccgtctta tggagcaaaa cccgcagtca cagctgaaac ttcttgtcac ccgtggtaag
     6541 gagcaaggct atctgaccta tgccgaggtc aatgaccatc tgccggaaga tatcgtcgat
     6601 tcagatcaga tcgaagacat catccaaatg atcaacgaca tgggcattca ggtgatggaa
     6661 gaagcaccgg atgccgatga tctgatgctg gctgaaaaca ccgcggacga agatgctgcc
     6721 gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa tcgggcgcac gactgacccg
     6781 gtacgcatgt acatgcgtga aatgggcacc gttgaactgt tgacccgcga aggcgaaatt
     6841 gacatcgcta agcgtattga agacgggatc aaccaggttc aatgctccgt tgctgaatat
     6901 ccggaagcga tcacctatct gctggaacag tacgatcgtg ttgaagcaga agaagcgcgt
     6961 ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag aagaagatct ggcacctacc
     7021 gccactcacg tcggttctga gctttcccag gaagatctgg acgatgacga agatgaagac
     7081 gaagaagatg gcgatgacga cagcgccgat gatgacaaca gcatcgaccc ggaactggct
     7141 cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa cgcgtgacac catcaaagcg
     7201 aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga aactgtctga agtattcaaa
     7261 cagttccgcc tggtgccgaa gcagtttgac tacctggtca acagcatgcg cgtcatgatg
     7321 gaccgcgttc gtacgcaaga acgtctgatc atgaagctct gcgttgagca gtgcaaaatg
     7381 ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa ccagcgatac ctggttcaac
     7441 gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc acgatgtctc tgaagaagtg
     7501 catcgcgccc tgcaaaaact gcagcagatt gaagaagaaa ccggcctgac catcgagcag
     7561 gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga aagcccgccg tgcgaagaaa
     7621 gagatggttg aagcgaactt acgtctggtt atttctatcg ccaagaaata caccaaccgt
     7681 ggcttgcagt tccttgacct gattcaggaa ggtaacatcg gcctgatgaa agcggttgat
     7741 aaattcgaat accgccgtgg ttacaagttc tccacctacg caacctggtg gatccgtcag
     7801 gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc gtattccggt gcatatgatt
     7861 gagactatca acaaactcaa ccgtatttct cgccagatgc tgcaagagat gggccgtgaa
     7921 ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg aagacaagat ccgcaaagtg
     7981 ctgaagatcg ccaaagagcc aatctccatg gaaacgccga tcggtgatga tgaagattcg
     8041 catctggggg atttcatcga ggataccacc ctcgagctgc cgctggattc tgcgaccacc
     8101 gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc tgaccgcgcg tgaagcaaaa
     8161 gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc acacgctgga agaagtgggt
     8221 aaacagttcg acgttacccg cgaacgtatc cgtcagatcg aagcgaaggc gctgcgcaaa
     8281 ctgcgtcacc cgagccgttc tgaagtgttg cgtagcttcc tggacgatta atcggtaggc
     8341 cggatcaggc gttacgccgc acccggcact agtctctctg cacaaacgcc accttt
//
LOCUS       DAEEGA010000134         8365 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEEGA010000134 DAEEGA010000000
VERSION     DAEEGA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.134"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..159
                     /locus_tag="KLH46_003670"
     CDS             <1..159
                     /locus_tag="KLH46_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380206.1"
                     /translation="ISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGE
                     DVEPGDDF"
     gene            complement(168..566)
                     /gene="vapC"
                     /locus_tag="KLH46_003671"
     CDS             complement(168..566)
                     /gene="vapC"
                     /locus_tag="KLH46_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HBE0380207.1"
                     /translation="MLKFILDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="KLH46_003672"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="KLH46_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HBE0380208.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..1246
                     /locus_tag="KLH46_003673"
     CDS             875..1246
                     /locus_tag="KLH46_003673"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HBE0380209.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALAST"
     gene            1267..6128
                     /gene="traI"
                     /locus_tag="KLH46_003674"
                     /pseudo
     CDS             1267..6128
                     /gene="traI"
                     /locus_tag="KLH46_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6148..6894
                     /gene="traX"
                     /locus_tag="KLH46_003675"
     CDS             6148..6894
                     /gene="traX"
                     /locus_tag="KLH46_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBE0380210.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6949..7509
                     /locus_tag="KLH46_003676"
     CDS             6949..7509
                     /locus_tag="KLH46_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBE0380211.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7640..7852
                     /locus_tag="KLH46_003677"
     CDS             7640..7852
                     /locus_tag="KLH46_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBE0380212.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8096..8335
                     /locus_tag="KLH46_003678"
                     /pseudo
     CDS             8096..8335
                     /locus_tag="KLH46_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
BASE COUNT         1925 a         2090 c         2682 g         1668 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggcagca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gaataaactt cagcatcaga atgactccct ttcctgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc actggcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcacggcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaaactggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggggc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aacgccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt
     4981 ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg
     5041 acctggccta tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg
     5101 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     5161 ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     5221 atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg
     5281 accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg
     5341 cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt
     5401 ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta
     5461 acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc
     5521 ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc
     5581 agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg
     5641 cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga
     5701 atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc
     5761 cgggagccgg aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag
     5821 aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga
     5881 acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg
     5941 agcgtgaccg ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg
     6001 aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc
     6061 gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg
     6121 gagactgata caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac
     6181 gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga
     6241 cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg
     6301 atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt
     6361 gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac
     6421 cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg
     6481 tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa
     6541 acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg
     6601 tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac
     6661 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     6721 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     6781 accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt
     6841 ttcccgacgt tctatgcatg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     6901 tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag
     6961 aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg
     7021 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     7081 gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg
     7141 tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg
     7201 ccgggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta
     7261 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     7321 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     7381 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg
     7441 cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     7501 gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa
     7561 taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca
     7621 actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg
     7681 gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga
     7741 agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt
     7801 tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa
     7861 gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta
     7921 atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta
     7981 agtcgatttt ttatattttg tgattagttt cacaaaataa ggtgttattc aatgtgtgct
     8041 gcactattca ggggggctga aatacaggtg gcatttatct gatatggaga ataacatgag
     8101 gaaatatatt ccactggtat tatttatctt ttcatggccg gtattgtctg cagatattca
     8161 tggacgggtt gttcgtgtac tggacggtga taccatcgag gtcatggact cactgaaggc
     8221 ggtgcggatc aggctggtaa atattgatgc accggaaaag aaacaggatt atggccggtg
     8281 gtcaacagac atgatgaaat cactggtcgc aggaaaaacg gttaccgtca catattgagg
     8341 tgtactggca atagcggaca ctacc
//
LOCUS       DAEEGA010000135         8356 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEEGA010000135 DAEEGA010000000
VERSION     DAEEGA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.135"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            66..314
                     /locus_tag="KLH46_003679"
     CDS             66..314
                     /locus_tag="KLH46_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBE0380213.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            379..747
                     /locus_tag="KLH46_003680"
     CDS             379..747
                     /locus_tag="KLH46_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBE0380214.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            841..1494
                     /gene="nfsB"
                     /locus_tag="KLH46_003681"
     CDS             841..1494
                     /gene="nfsB"
                     /locus_tag="KLH46_003681"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBE0380215.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            1602..2849
                     /gene="ybdG"
                     /locus_tag="KLH46_003682"
     CDS             1602..2849
                     /gene="ybdG"
                     /locus_tag="KLH46_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBE0380216.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(2917..4293)
                     /gene="pheP"
                     /locus_tag="KLH46_003683"
     CDS             complement(2917..4293)
                     /gene="pheP"
                     /locus_tag="KLH46_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBE0380217.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(4394..7537)
                     /gene="cusA"
                     /locus_tag="KLH46_003684"
     CDS             complement(4394..7537)
                     /gene="cusA"
                     /locus_tag="KLH46_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBE0380218.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(7549..8067)
                     /gene="cusB"
                     /locus_tag="KLH46_003685"
                     /pseudo
     CDS             complement(7549..8067)
                     /gene="cusB"
                     /locus_tag="KLH46_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2042 a         2325 c         2031 g         1958 t
ORIGIN      
        1 gccgggaatt gccttacaga agaatatccc tgacaatggt gttttaactt acatttaaca
       61 atgttatgaa acacccttta gaaaccttga ccaccgcagc aggcattttg ctgatggctt
      121 tcctctcttg cctgctgctg cccgcccccg cactggggct gacgctggca caaaaactgg
      181 tgaccatgtt ccatctgatg gatcttagcc agctttacac tttattgttt tgtctgtggt
      241 ttttagtgct gggcgctatt gagtattttg ttctgcgctt tatctggcga cgctggttct
      301 cgctggcgga ttaaacgtcg attatcgccg aacttcgcgg catactttgc ttattctcct
      361 tcgcctaaag gaacgtttat ggataagcaa tcactgcacg aaacggcgaa acgcctagct
      421 cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga tgtttttaaa
      481 attggcggca agatttttat gctgtcgtcg gagctacgcg gcgtcccctt tatcaatctg
      541 aagtccgatc cacaaaaatc cctgttaaat cagcaaatat acccaagcat taagccaggg
      601 tatcacatga ataaaaagca ctggatttcg gtgtattccg gcgaggaaat ctccgaagcg
      661 ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc
      721 gatcaaaaaa gagtgcgtcc aggctaaagc gggaatctat agcgcatttt tctcgcttac
      781 catttctcgt tgaaccttgt aatctgctgg cacgcaaaat tacttacaca tggagtcttt
      841 atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga tgccagcaaa
      901 aaacttaccc cggaacaggc cgagcagatc aaaacgctac tgcaatacag cccatccagc
      961 accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt
     1021 gccaaatccg ctgccggtaa ttatgtgttc aacaaacgta aaatacttga tgcctcgcac
     1081 gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct ggttgttgac
     1141 caggaagatg ccgatggccg ctttgccacg ccggaagcga aagccgcgaa cgataaaggt
     1201 cgcaagttct tcgccgatat gcaccgtaaa gatctgcatg atgatgcaga gtggatggca
     1261 aaacaggttt atctcaacgt cggtaacttc ctgctcggcg tggcggcgct gggtctggac
     1321 gcggtgccca tcgaaggttt tgacgccgca atcctcgatg cagaatttgg tctgaaagag
     1381 aaaggctaca ccagtctggt ggttgttccg gtaggtcatc acagcgttga agattttaac
     1441 gctacgctgc cgaaatctcg tctgccgcaa aacaccacct taaccgaagt gtaattctct
     1501 cttgccgggc atctgcccgg ctatttcccc tcagattctc ctgatttgca taaccctgtt
     1561 tcagccgtca tcataggctg ctgttgtata aaggagacgt tatgcaggat ttaatatccc
     1621 aggttgaaga tttagcgggt attgagatcg atcacaccac ctcgatggtg atgattttcg
     1681 gtattatttt tctgaccgcc gtcgtggtgc atattatttt gcattgggtg gtactgcgga
     1741 ccttcgaaaa acgtgccatc gccagttcac ggctttggtt gcaaatcatt acccagaata
     1801 aactcttcca ccgtttagct tttaccctgc aggggattat cgtcaatatt caggcggtat
     1861 tctggctgca aaaaggcacc gaagcggcag atattctgac tacctgcgcg cagttgtgga
     1921 tcatgatgta tgcgctgctt tcagtcttct cgttgctgga tgttattttg aatctggcgc
     1981 agaaattccc ggcggcatct cagttaccgc tgaaagggat atttcagggg attaaactga
     2041 tcggcgcaat cctggtcggc attttgatga tctcgctgct gattggtcag tcgccagcga
     2101 ttctgatcag cggtcttggt gcaatggctg ccgtgctgat gttggtattt aaagatccga
     2161 ttcttggtct ggtggcaggt attcagcttt ccgcgaacga tatgctgaaa ctgggcgact
     2221 ggctggaaat gccgaaatac ggcgcggatg gcgcggtgat cgatattggg ttaaccaccg
     2281 tcaaagtgcg taactgggac aataccatta ccactattcc cacctggtct ctggtttctg
     2341 actcctttaa aaactggagc gggatgtcag catctggcgg gcgacgtatt aagcgcagta
     2401 tcagtattga tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa cgtctgaata
     2461 aagcgcattt gttaaagcct tatttaacca gccgccatca ggaaattaat gagtggaatc
     2521 gccagcaagg ttctacggag tcggtattaa acctgcgccg aatgaccaat attggaacct
     2581 ttcgtgccta tctgaacgaa tatctgcgta accatccgcg gattcgtaaa gatatgacct
     2641 taatggtacg ccaactggct ccaggtgata acggtttacc gctcgagatc tacgcgttta
     2701 ctaacaccgt ggtgtggctg gaatatgaaa gcattcaggc tgatatattc gatcacatat
     2761 ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc gccaaccggc aatgatattc
     2821 gctctctggc gggtgcattt aagcagtaat taaaaaaacc gctctcatcg aatggatgag
     2881 agcggacatc gttttgtcgg atgtagcgtg aatgccttat ttccgacgca gcgttttaaa
     2941 tgccacaaac aggaatacaa tccacaccgg cagcaggatc gctgacaagc gcatatcatc
     3001 catcgtgcac atcagcagca aaatcatgcc gaggaaggcg atgcaaagat agttgccgaa
     3061 cggatagagc agcgccttaa actgtgtttc acgcccctga cgtcgcatcg ccgcgcgaaa
     3121 acgcagatgc gccaggcaga tcataatcca gttcaacagc aacgttgcta ccaccagcgc
     3181 catcagcaga ccaaacgctt tttgcggcag cagatagttg attaacacca ccagcgaagt
     3241 gatcgctccg gaaagcatca gcgagttaat cggcacaccg cgacggctga cgcgagtcaa
     3301 aaacttcggc gcattaccct gcacagaaag gccaaacagc atgcggctgt tagagtaaac
     3361 cccgctgtta tacaccgaca gcgatgccac tagaatgacg aagttcagcg cagaagctac
     3421 cacattgctg tcgagattat ggaaaatcat cacaaacggg ctactgttgg atttcacttc
     3481 cacccacgga tagagtgcca gtaaaaccac cagtgaaccg atataaaaca gcaggatgcg
     3541 atacaccacc tgatttaccg cttttggaat gcttttttcc ggatcgcgcg cttcagcggc
     3601 tgtaatccca atcagctcca gaccgccgaa ggagaacata attaccgcca gcgacaaaat
     3661 cagcccattc cagccggtgg cgaagaaacc accgtagcgc cagaggttgt cgatactggc
     3721 tttctcgccg ccgtaaccag aaaacagcag ccacaggcca aagccgatca taccgatgat
     3781 cgccagcact ttaatcagcg caaaccagaa ctcggtttcg ccatataagc gcacgttcac
     3841 caggttaacg gcgttgatga taataaagaa ggcggcagcc caaatccacg ttggaacatc
     3901 cgggaaccag tactgcatat agatgcccgc agcggtcagc tctgccattc ccaccagcac
     3961 gaacattacc cagtagttcc agccagagag gaagcccgca aacggtcccc agtatttata
     4021 ggcaaagtgg gcaaatgaac cggataccgg ctcttcgacc accatctcgc caagctggcg
     4081 cataatcagg aaagcgatga tcccggcgac gccgtagccc agcaatacag ccggacccgc
     4141 catctgaatc gccgggccaa tgccaagaaa cagaccagta ccaattgcgc cacccagcgc
     4201 aatcagttga atatgacggt tatgtaatcc gcgatgaagc gtcggctctt gattcgacgc
     4261 agtatcttcc gatacggttg acgcgttttt cacgcctttc ccctgtgtgt cttttttgtt
     4321 gaggggcacc ttttaacatt tagtgcccat cgtcgcaaga cacaatccac acggttaaac
     4381 gaggtatcct gctttaattc cgtacccgat gtcggtgcag ccacatcagc ttatacgccg
     4441 ccgggataat aaacagcgac agcaaaggtg cggtgatcat gccgccaatc atcggtgcgg
     4501 caatccggct catcacctct gaaccagccc ccgttcccca cagaatcggc agcagaccgg
     4561 cgataatcac cgccaccgtc atcgctttcg ggcgcacgcg caggaccgcg ccgtgataca
     4621 acgcctcatc cagcttctgc tcgctgaatg tttgtggatt attcaacgac ggctcggcct
     4681 ctatggcgtg acgtaaatac atcagcatca ccacgccaaa ttcggcggcg accccggcaa
     4741 gggcaataaa gccggtaccc gtcgccacag aaagatgaaa gcccatccac cacagtaacc
     4801 agatgccgcc caccagcgca aacggtacgc tgctgataat cagcaacgct tcgcccaccc
     4861 gacggaatgc cagatacaac agcacgaaga tgatcatcag tgtcatcggc accatgagtt
     4921 tcagcttatg gttggcgcgc tccagtagct cgaactgccc ggagaatgcc acgctggtgc
     4981 ccggttttaa ctgcactttt tcagctatcg ctttttgcaa atcgtgaacc accgacacca
     5041 tgtcacgatc gcgggcatcg atataaatcc agctcgtcgg gcgcgcattc tcggttttca
     5101 gcatcgacgg tccggtagag actttaacgt cggccacgtc tgccagggtg atttgctgct
     5161 tcatcggcgt caggatcggc aactggcgca gtgcctgcgg actatcgcgc cagctttgcg
     5221 gataacgcag attaattgga taacgggcaa tcccttccac cgtttcgcca accatcgccc
     5281 cgcccaccgc agaagtcaca aacaactgca catccgccac cgtcataccg taacgtgcgg
     5341 ctttttcacg gttaatctca acgttgatat agcgcccacc ttccagccgc tcagcaagcg
     5401 cagaagctac gcctggcacc gttcgcgcca cttcttcaat ttgctcagcc atcgcgtcga
     5461 tatccgccag cacagtgccg gaaactttaa tgccgatggg gcttttaatg ccggttgaga
     5521 gcatatcgat acggttacga attggcggca cccacagatt cgccagcccc ggcagacgca
     5581 cggtgttatc cagttcctca atgattttgt ccattgtcat gcctggccgc cactgatcct
     5641 gtggcttaag ctggatggtc gtttctacca tctccagcgg agcagaatcg gtggcggttt
     5701 ccgctttccc ggttttgcca aatacccgcg ccacttcagg tacgctcata attagcttgt
     5761 cggttttttg cagcatactc gccgcctctg ctgcggaaat ccccggcagc gtcgatggca
     5821 tatacaacaa gtcgccttca ttgatctgcg gtaaaaattc cccgccaact ttattgagcg
     5881 gccagagaac cgtcagcacc gaaagcgccg ccaccagcag cgtggttttc ggccagtgca
     5941 gtactttcag caacagcgga tgataaacac gaatcaaaaa gcgattgagc ggattactgc
     6001 tttccggcgg aattttgcca cggatccagt aacccatcag gatagggatc actacgatcg
     6061 ccagcagcgc cgcacccgcc atcgcatacg ttttggtgaa cgccaacggg ccaaacagac
     6121 gcccttcctg cccttccagg gtgaagatcg ggataaacga caacgtgata atcagcagac
     6181 tgataaacag cgccggcccc acttcaacag acgcatcggt gatcacctgc cagcgcgttt
     6241 tattatccag cgtggcgtca ggatgctggt gctgccactc ttccagccgt ttatgcgcat
     6301 tctcgatcat gacgatagcg gcatcgacca tcgccccgac ggcaatcgca atgccaccca
     6361 gcgacataat attggcattc agcccctgga agggcatgac aataaaagca atacacaacc
     6421 ccagcggcaa cgaaataatc gccaccagcg ccgagcgcac atgccagaga aacagcgcgc
     6481 agactaccgc caccacaata aactcttcca gcaacttgcc gctgaggttg tcgatggccc
     6541 ggtcaatgag ctggctgcgg tcgtatgttg taactatctc cacgccttcc ggcagactac
     6601 ttttcagcgt ttccagtttg tccttcacgg cggcgatcac ttctcgggcg tttttgccgg
     6661 atcgcaggat caccaccccg cccgccactt cgccttcgcc gtttagttcg gcaatgcccc
     6721 ggcgcatctt cgggccaatc tggaccttcg caacatcgcg cagataaacg ggcacgccat
     6781 tttcactggc ttttaaaacg atgtgattaa agtcgtcgag cgtttgtaga tagccgctgg
     6841 cgcgcaccat atattctgct tccgccagtt cgattgacga accgcccgct tcctggtttg
     6901 aagcatccag cgcgcttttt acttcggcga gactgatgcc atactgcgcc aggcgctgag
     6961 gatcgataac cacctgatac tctttcacca caccgcctac cgacgccact tccgcaacgt
     7021 caggaatggt tttcagctca tatttgagaa accagtcctg caatgagcgt aaatcggcaa
     7081 gatcgtgctt accgctgcga tccaccagtg catattcata gatccagcca acacccgtgg
     7141 catctggtcc cagctcggcg ctgactcccg caggcagctt accctgtacc tggttgaggt
     7201 actccagcac ccgcgaacgc gcccagtacg gatcggtgcc atcttcgaaa atgacataca
     7261 cataagagtc gccaaactgc gagaaaccac gcacagtctt cgcgccaggc accgacaaca
     7321 tggtggtggt tagcggataa gtcacctgat tttcaacgat ttgcggtgcc tggccgggat
     7381 agctggtttt aataatcacc tgcacatcgg agagatccgg caacgcatcc actggcgtat
     7441 taatgatggt ccaggtgccc cagatgctca gaaacaacgc gcccatcagc accaaaaaac
     7501 ggttcgccac cgagcgacga ataatccatt caatcattgg ttattccctc aatgcgcatg
     7561 ggtagcactt tcagagcgta tccgctccag tgcgccagaa atattggctt cggaatcaat
     7621 caggaacagg ccgccggaaa ccaccttttc accttccgcc agaccagagc gtaatgcggt
     7681 gacgccttgc gatgcctgga aaacagcaac gcgtttcggt acaaagcgcc cgtcggcatc
     7741 aacggtaatc acccgctgtt cgttgccggt atcaatcagc gcctgtgacg gaatgagcag
     7801 catcggttcg ctggcggtgt tgagttgcaa ccaggcgttc atgcccggtt ttagcgcctc
     7861 gtcggcgttg tcgacttcca gacgcagctg cagcgtgcgg gtcgcggcat ccacgccagg
     7921 tagcagcgtc catttgcgga tggtgagtgt tttatccggt cgcgccggaa cggtgagggt
     7981 aaactgcgag gcatctttca ccagccaggc gatggactcc gggatcgcag cagtgaccca
     8041 caccgggtcc ataccctgaa ttcgcgatta cgcgcaatcc ccctgagaac cgccatgaac
     8101 aagggtttca tgatttgtga ttttagtttg gtacgcaatt tggtacacaa cactattttc
     8161 acttcagcgg gtagtcatca aactcaccat aacgagcatc gttgatgatg tatgtgatca
     8221 ccacaaatat cacatctgga ctagttccgt cttcatgcgc aggtagactg gccccctgaa
     8281 tctccagaca accagtatca cttatttaag tgatagtctt aatactagtt tttagacttg
     8341 tcattggaga acagat
//
LOCUS       DAEEGA010000136         8321 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEEGA010000136 DAEEGA010000000
VERSION     DAEEGA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.136"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..1153
                     /locus_tag="KLH46_003686"
     CDS             <1..1153
                     /locus_tag="KLH46_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HBE0380219.1"
                     /translation="MPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRAD
                     DLRDERSYRRRIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVS
                     DAERLMFSRELNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAG
                     FDEILRSPKLPAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTT
                     GFKRGDKISFIGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKE
                     EKAYANVNTSLADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSF
                     SIPGIGVNGIFATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            1198..1422
                     /locus_tag="KLH46_003687"
     CDS             1198..1422
                     /locus_tag="KLH46_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380220.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEEKQCKKPGRKPKAAADEPDNEG"
     gene            1400..1900
                     /locus_tag="KLH46_003688"
     CDS             1400..1900
                     /locus_tag="KLH46_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HBE0380221.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            1900..3318
                     /locus_tag="KLH46_003689"
     CDS             1900..3318
                     /locus_tag="KLH46_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HBE0380222.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            3318..4115
                     /locus_tag="KLH46_003690"
     CDS             3318..4115
                     /locus_tag="KLH46_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380223.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATTIKIIAEQ"
     gene            4115..4570
                     /locus_tag="KLH46_003691"
     CDS             4115..4570
                     /locus_tag="KLH46_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HBE0380224.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            4573..5181
                     /locus_tag="KLH46_003692"
     CDS             4573..5181
                     /locus_tag="KLH46_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HBE0380225.1"
                     /translation="MLYAFKLGRKLCGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGAPQGQANAG
                     QTYANNMSSIAQQSAALASLLGTSTPWGAGIGAGIGLLGSLF"
     gene            5191..6522
                     /locus_tag="KLH46_003693"
     CDS             5191..6522
                     /locus_tag="KLH46_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HBE0380226.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            6523..8253
                     /locus_tag="KLH46_003694"
     CDS             6523..8253
                     /locus_tag="KLH46_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBE0380227.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDALQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT         2201 a         2045 c         2177 g         1898 t
ORIGIN      
        1 gatgcctgtt gagcaggaag cgccaaccca gactggctgg gatttaactg gcaacgcaac
       61 cgggattctg gaactctccg tgaagtgcaa catgggcgat ccggataacg atttcttcga
      121 gcttcgtgca gatgacctgc gtgatgagcg ttcttaccgt cgccgcatcc aggcatccgc
      181 caaaaaactg gcgaataaca ttgagtcagc gattgccaaa caggcaactg aaatgggctc
      241 gcttgttgtt cacgataccc gcgcaattgg tccatcgact ggcctgcctg gctgggattt
      301 tgtgtctgat gcagagcgac tgatgttctc ccgtgagcta aaccgcgaca tgggcatcag
      361 ttacttcctg aaccctgacg attaccgcaa agcaggccgc aacctggtag atggtgacat
      421 cttcgggcgc gttcctgaag aagcatatcg aaacggtact attcagcgtc agattgctgg
      481 ctttgatgaa attcttcgct caccgaaact tccggcagtt accaagtcaa ccgcgactgg
      541 tgtaactgtt tctggtgcgc agaagtttaa gccgcaggca tacacccttg ataccgatgg
      601 taacaaagag aacgtcgaca accgtgttgc aacggtgacc gtatcctcca ccaccggatt
      661 taagcgcggc gacaaaatca gcttcattgg tgtgaagttc ctgtctcaga tggcgaagaa
      721 tgtgctgact gatgacgcga ctttctcaat cacccgtgtg atcgatggta ctcacatcga
      781 aatcacgccg aagccgattg cgctggatga cgcgtcactg acaaaagaag agaaggctta
      841 cgctaacgta aacacctctc ttgctgatac cactccggta aacgttctga acgtggcaac
      901 aaccaccgct aacgtgttct gggctgatga ctcaatccgc ctgctgtctc agccgatccc
      961 ggtaacccat gaactgtttg ctggcatgaa aacgtcttcc ttcagcattc caggtattgg
     1021 tgttaacggc atcttcgcaa cgcagggtga tatcaacact ctgtctggta agtgccgtat
     1081 tgctgtgtgg tattcagcat gtgctgtacg accagaggca attggtgttg gtctgcctaa
     1141 ccagaccgcg tgataaccag agggagcttc ggctcccgtt tttatctgga gacaagcatg
     1201 acacacatga tctttcgtca tggcgacatg aagaagtgga aaggcgttgg ctacgacttt
     1261 gaaatcgtga aagccgaaga gcttcaggaa tatctggatg ctggctggtt ttcacatcct
     1321 gatgaccttt tgaaggatgt tgcagagcca gaagaaaaac agtgtaaaaa gcctggtcga
     1381 aaacctaagg cggcagcaga tgaacctgac aacgaagggt gatttagttc ttgcggcatt
     1441 acgtaagctc ggtgttgcat caaatgccac gttaaccgat gtcgaaccgc agtctatgga
     1501 agacggcgtc aacgaccttg aaatgatgat ggctgaatgg cttggcggtg atgcgtcacc
     1561 tggtatcaac gttggctaca tttttgctga tgcagatgtt gctccggatc cgggcgatga
     1621 gcacggttta tcaaataacg ctatcaatgc cgtcattttc aaccttgcct gccgaattgc
     1681 tccagattat gcgctggaag cgtctgcaaa acttataacc actgccagat acgggaaaga
     1741 gcgactcgtc aaactgtctg caatggacag ggcaaaagca gctaaatgta agtccggtta
     1801 tccaaaccgt atgcctgttg gtagtggtaa ccagttggcg aagtggaatg gttggaatta
     1861 cttccaccga aaggaacctt gcgataacgg gagcgaataa tgcctattca gcaacttccg
     1921 cttatgaaag gtgtcggcaa agacttccga aacgccgact atatcgacta tctgccagtg
     1981 aatatgctgg ctacacccaa agaaatcctg aacagcagcg gatatcttcg ctcattcccg
     2041 ggcattgcca aacgttctga cgtgaacggt gtatctcgcg gcgtcgagta caacatggcg
     2101 cagaatgctg tttatcgcgt gtgtggcggc aagctgtaca aaggagaaag tgaagtcggt
     2161 gatgttgccg gaagtggtcg tgtatcaatg gcgcatggtc ggacatcaca ggcggtaggc
     2221 gttaatggtc aactggtcga gtatcgctat gatggcacgg tcaaaaccgt ctcaaactgg
     2281 cctacagaca gtggattcac tcagtatgag ttaggttcag ttcgcgacat tacgcgctta
     2341 cgtgggcgtt atgcgtggtc aaaagacgga actgattcat ggtttatcac tgaccttgaa
     2401 gacgaatctc atcctgaccg ttacagcgca caatatcgtg ccgagtctca gcctgacggc
     2461 atcatcggta tcgggacatg gcgagacttc atcgtctgct ttggttcatc gactattgaa
     2521 tatttctccc tgactggcgc aaccaccgtt ggtgccgctt tgtatgttgc acaaccatcg
     2581 ctgatggtgc agaaaggcat tgccggaact tactgcaaaa cgccattcgc tgattcgtat
     2641 gcgtttatca gcaatccggc aacgggtgcg ccgtctgtgt atatcatcga ctccggtcag
     2701 gtgtcaccaa ttgccagcgc gagcattgag aaaatcctcc gctcctacac tgctgatgaa
     2761 ctggctgatg gtgtgatgga atcgctgcga tttgatgctc atgagttgct gattatccac
     2821 cttccgcgtc acgtcctcgt gtacgacgca tcttcaagcg ccaatggccc acaatggtgt
     2881 gtgctgaaaa caggtctgta tgacgatgtg taccgcgcta tcgacttcgt ttacgaaggc
     2941 aatcagataa cgtgcggcga taagctggaa tctgttaccg ggaaactgca attcgatatc
     3001 agcagccagt acgacaagca acaggaacac ctgctgttta ctccactctt caaagcggat
     3061 aacgccagag tgttcgacct tgaggttgaa tcgtcaactg gcgttgcgca gtacgccgac
     3121 cgcctttttc tctctgcaac cactgactgc atcaattacg ggcgtgagca gatgattgag
     3181 cagaatgaac cgttcgttta cgacaagcgt gttttgtgga agcgagtagg gcgcatcagg
     3241 aaaaatgtcg gcttcaaatt gcgcgttatc acgaagtcac ctgtcactct gtctggcgct
     3301 cagataagga ttgagtaatg gctgattcga atctcaatgt gccggtaatc atccaagcta
     3361 cgcggctcga tacatcagtc cttccacgca atatcttctc gcagtcgtat ctgctttacg
     3421 ttatcgcaca gggcactgat gttggtaacg tggctaacaa agccaacgag gccggacagg
     3481 gcgcttatga tgcacaggtc aggaacgatg agcaggatgt gattctcgct gaccatgagc
     3541 agcgaatttc tgctgcggaa gcaacgcttg ttaatcatga ggagcgaatc agacaggcag
     3601 aatcaactct tcaggaccat gaaacacgaa tagctcagaa tgaaagcgat atttcgtcgc
     3661 ttgacacaag agttcagtcg ctggagtcgc aggtttcaga ccatgaaacg cgcatcgatg
     3721 ctctggagta tgcaaccaca cgcaagaagt cagaggttgt ttactctggt gtatctgtaa
     3781 ccatcccgac agcgccgacc aaccttgtta gcctgctgaa aacgctcaca ccgtcatccg
     3841 gctcgttggc accattcttc gacaccgtta acaacaagat ggttgtgttc aacgagaaca
     3901 aaaccctgct cttcaagttg tcgattgtcg gcacgtggcc cagcggaacc gccaacaggt
     3961 caatgcagct aaccttttcc ggctctgttc ctgacacact ggtcagcagt cgtaatgcgg
     4021 cgacaacaac cgacaacatc ctgttagcta cgttcttcag cgtggataaa gacggctttc
     4081 ttgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac caacgcgaaa
     4141 catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga gcaacaacgg
     4201 tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc acggtcagtt
     4261 tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc acgccatgta
     4321 cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc gatacattct
     4381 gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc gacacggtca
     4441 gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat acttcaaagg
     4501 cgtggatgac gtgacttttt acagcgccac acgcgaagaa ctaatcgact tcctgaatca
     4561 cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgtgcgg cgaggaacct
     4621 tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa gtatgcagca
     4681 gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat catgaaaaac
     4741 cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa cctttccagt
     4801 ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta taaagacctt
     4861 gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg acttggttcg
     4921 acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc ttggttatca
     4981 aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgcc gcaaggtcag
     5041 gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca aagcgcagct
     5101 cttgccagcc ttttgggaac atcaacgcct tggggcgctg gtattggtgc tggtatcgga
     5161 ttgcttggct cgttgtttta aggggtaatc atggctactt ggcaaggaac aaacggcgga
     5221 ttgttggctg gtatcggcgg cgtcaactca aacgctccga gcgtaaatga catcggcaat
     5281 acgcttcagc ttatcaggca gaacaatgat attgagcgtt caggcgctaa caatgttggg
     5341 ctgactgctt tgcaaggtct ttcaggtatt gcgggtgttt ttcagcagga aaagcaggct
     5401 cagcggcaga aagaatttca gcaggcgtac gctaatgctt atgcgtctgg tgatcgcggt
     5461 gctttgcgtc agttggctac tcaatatcca gaccagattg aatccgttcg taaaggcatg
     5521 ggattcattg atgaagagca gcgtaattct atcggcacct tagcggctgg cgcacgcctt
     5581 gcgtcatcgt ctccagaagc aatgcaatca tggctgcaaa acaacgccgg tgagttagct
     5641 cgtgttggcg ttaatcctca tgacgtcgct cagatgtacc aacagaaccc gcggcagttc
     5701 ggcgaatttg tcgatcacct ggggatgaac agtctcgggc ccgaaaaata ctttgaccta
     5761 caggataaaa tgcagggtcg ccaggttacc atgcgcggtc aggatctgga ttcgcaaacc
     5821 gccgctcgga atcaggcaat cacaatgcgc ggacaagata tccaggcgaa tttaggtcag
     5881 cagcgcatta atctggacgc agaaacaaac cgcattaaca acgaaaataa gcgccttgac
     5941 cggatgctat cagcagaaac taacgacctg aagcgccagg aaatacagag ccgcatagca
     6001 gccaacaacc agcagttgca gcagaagcag caagcgctaa atgatggcta caaagacggc
     6061 atcaacaccc tcacaaccag catgttcact ctgaacgata tcgttagttc tccttcactt
     6121 aagagcatta caggcttacg tggagtaatc cccaacgttc caggctcaca ggctgcagac
     6181 actcaggcac gacttgatac ctttaaatca caagcatacc tgacagcggt tcaggccatg
     6241 cgaggcatgg gcgcactttc tgatgccgag ggcaaaaagc tcgaccaggc tgttggttcg
     6301 ctgcagaact cgcagagcga ggagtccttt cgtcgcaacg ctggcgtcat cctgaacacg
     6361 ctcaaccaga agcgtaatga ggcggttggt aagtacgttc agcaaaacgg tatcaagcga
     6421 gtggaagcgc ctcaggcttc tatagattac ctgaagcagc accccgagct gtcaatcgac
     6481 ttcattaatc gctacggata tcttccatct ttggggcagt aaatggctaa ttaccgtgat
     6541 ttgttagagc aggctggcgc acgttacggt gtgccagaag ggttgatgac tgcactgggt
     6601 gccaaggagt cttcttacaa ccctgccgca gtaagctccg ccggggctgt aggattgact
     6661 caggtcatgc ctgggacatg gcgtgatatg ggttataccg atgagcaaat gcaaaacccc
     6721 gaatatcagg ctgacgctgg cgcgcgctat ctggcaaaga tgtaccagca gtttggtaac
     6781 tggcgtgacg ctcttcaggc ttatcacgac ggtcccggca acgttatgaa ggcaaagcgt
     6841 ggtgaatata cgccaggacc tgaaggccgc ggttacgttg atgatcgctt tgctcaatgg
     6901 gctggtgacc cggtgacaga ctcaacagtc gaacagcgcg ccacctctgc aaaggtacat
     6961 cctcagcaag accctaacaa cccgtttgca caactggaag tacagtcatc cgaacaagta
     7021 tcggcatcag gtgtgcagtc agacccaaat aatccatttg ctcagattga gcagcaggca
     7081 gccagtcagc agccacctca acccgtaagt tctgtcgcac cgaaacctgt tcagcaacaa
     7141 acagttaatc aggccaataa tgcaccaaca cgggaagaac catcattgat gcaacaagct
     7201 ggcgattggc tcactggtgg tcaaagtgca gggcaaattg cagaacaggc tggtcgtggt
     7261 ctggtaaaca taccatttga cgtattgcag ggtggcgcaa gtctgattaa tgcaatcagt
     7321 caggggcttg gggggccaaa agtattggat gatgtttatc gtccagtaga cagaccgaca
     7381 gacccctacg cgcaagctgg agagtcaata ggcggttatc ttgttcctgg agcaggtgta
     7441 gctggaaaca tggtcattgg ttctctcgct gacgcggcga atcaacgggg tgattttgcc
     7501 gaaaatgccg ctattaatgc cggacttagc attgctacgc atggcctgat aaatggcgtt
     7561 acccgtggtg ttcgtggtgc atcaaatata attagtggca ataaaacatc tgcacagaga
     7621 gcgaccactg cgccaacaga aacatcacca ttctccggtg atgccgctgc agcaacaaat
     7681 cctgcggttc atgccgcaga ggcaagagta gcacaaggtg taccaatgac gcctgcgacg
     7741 aagaacccag aggaagtcgt tcgcacagta gcagcacaaa aaaggccaaa tctcgcttca
     7801 tcgcttgatg aactagatat caatcctcag gctgaagttc tggagtctgc tgaaaggctt
     7861 aatgttgatt cattactccc ttcacacttt tccgggaacg agcaatacaa ggcagttgag
     7921 caagcaatca agtcccgtgc gggttctgct ctacaggtgc aggaaaatga agcaatcagg
     7981 cagctagcac agggcgcggg tgagataatt gatcgcgttt ccggtgcaaa agatgctctt
     8041 ggtatgagcg acaagtttat tgatacggtc aatggaagaa tgtctgcgct gatgaaacga
     8101 agcgaccagc tttatcgcaa tgttgaaaag gcgatgcctg caggtgcaaa aattgatgcg
     8161 ccatcaacaa ggtcaatgct caaacaggtg gcagaagatc ttggcgggat gaaaaacctt
     8221 gaccctattg aaaagagagt tgaggtagcc tgagtttaac ggacactcct tcctgaaata
     8281 gaatggcatc agaaggagct aataatgagc agaaaaaccc a
//
LOCUS       DAEEGA010000137         8210 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEEGA010000137 DAEEGA010000000
VERSION     DAEEGA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.137"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..306
                     /locus_tag="KLH46_003695"
     CDS             <1..306
                     /locus_tag="KLH46_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBE0380228.1"
                     /translation="LLIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMAL
                     SSTLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            278..2206
                     /locus_tag="KLH46_003696"
     CDS             278..2206
                     /locus_tag="KLH46_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBE0380229.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            2190..2393
                     /locus_tag="KLH46_003697"
     CDS             2190..2393
                     /locus_tag="KLH46_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0380230.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2390..3983
                     /locus_tag="KLH46_003698"
                     /pseudo
     CDS             2390..3983
                     /locus_tag="KLH46_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3973..5478
                     /locus_tag="KLH46_003699"
     CDS             3973..5478
                     /locus_tag="KLH46_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBE0380231.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5515..5862
                     /locus_tag="KLH46_003700"
     CDS             5515..5862
                     /locus_tag="KLH46_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBE0380232.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5920..6948
                     /locus_tag="KLH46_003701"
     CDS             5920..6948
                     /locus_tag="KLH46_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBE0380233.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7000..7386
                     /locus_tag="KLH46_003702"
     CDS             7000..7386
                     /locus_tag="KLH46_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBE0380234.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7398..7775
                     /locus_tag="KLH46_003703"
     CDS             7398..7775
                     /locus_tag="KLH46_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0380235.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7762..>8210
                     /locus_tag="KLH46_003704"
     CDS             7762..>8210
                     /locus_tag="KLH46_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0380236.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPI"
BASE COUNT         1898 a         1934 c         2539 g         1839 t
ORIGIN      
        1 ctactcattg ccagatggga actgaccgca gaacaggctg tcgcacagca gttaaaaaat
       61 caggtgtcaa aaggaaatct tatcgatacc ggattttgta tttttgccct tagtaagctg
      121 gcgatggctt tatccagtac gcttgactcc attcctttgt ccatgcaacg acagttcccg
      181 gatttaacgc cgcgtcatat tgatcatctg aaaatcctta ttgctaaggg agcgaatcag
      241 tgcgcgcgtg cgggggataa attaccggat ttactcgatg aatatatcag aacaacaact
      301 gagtaatatg atggtggcag tcactatcgc actgcagcca ctggtcaggg tattgccggt
      361 gacggctgta gaatgggccg atcaaaatta ttacctgcct aaagaatcgt catacggtga
      421 gggggaatgg aaaacgctgc cgtttcaggt cgccatcatg aactgcatgg gcaatgacga
      481 gattcgtaca gttaacctga ttaaatctgc ccgtgttggt tatacaaaaa tggtgcttgg
      541 tgtgatcggg tattttattg agcacaaatc ccgcaacact cttctttttc agccgacgga
      601 ctcagctgct gaagatttta tgaagtctca cgtggaagca acaattcgtg atgttccttg
      661 cctgaaaaaa ctttctccct ggctgggccg gaaacaccgg gataatacgc tcacactgaa
      721 gcgtttttcc tctggtgtgg ggttctggtg tttgggtggt gctgctgcca aaaactaccg
      781 tgaaaaatct gtcgatgtgg tctgctatga cgaactctcc tcgtttgaac cggatgtgga
      841 aaaagaaggt tcgccaacac tgcttggcga taaacgtatt gaaggttcgg tatggccaaa
      901 atccatacgc ggttcaacgc caaaaattaa aggctcctgt cagattgaaa aagctgcgaa
      961 tgaatctgcg catttcatgc ggttttatgt cccttgccct cattgcgggg aggagcagta
     1021 tctgaagttt ggcgatgatg cgacgtcgtt tggcctgaaa tgggagaagg gtaaaccgga
     1081 aacggtgtat tacctgtgtg aacataatgg ctgcgtgatc cgtcagtctg aacttgatca
     1141 gaccgggggg cgctggattt gtgacaatac cgggatgtgg acgcgtgacg gtctgacgtt
     1201 tttcagtgct tcgggtaatg aaattccgcc gccgcgctcc atcactttcc atatatggac
     1261 ggcgtacagt ccgttcacca cctgggtaca gatagtctgt gactggctgg atgcactgaa
     1321 agatcccaac ggcgtgaaaa cttttgtgaa caccacgctg ggcgagacct gggaagaggc
     1381 cgtgggcgaa aaactcgatc accaggtact gatggataag gttgtgcctt acacggcaac
     1441 ggtgcctgtc cgggtggttt atctgacggc gggcattgac tcgcagcgaa accgttttga
     1501 gatgtatgtc tgggggtggg ctccgggaga ggaagccttt ctggtggata aaatcatcat
     1561 catgggacgt cctgatgagg aagagacgct gttacgtgtg gatgcggcga tcaataaaaa
     1621 ataccgccat gcagacggta ccgaaatgac catttcccgt gtctgctggg atatcggggg
     1681 gatcgatggc gaaatcgtgt atcagaggtc aaaaaaacac ggtgttttcc gggtactgcc
     1741 ggtaaaaggt gcctctgtct atggcaagcc ggtgatcacc atgccgaaaa cccgcaatca
     1801 gcggggtgtg tatctgtgtg aggtggggac ggataccgca aaagaaattc tctatgcccg
     1861 aatgaaggcc gatcccaccc ctgtgaatga ggccacatcg tatgccatcc gctttcctga
     1921 taatccggag attttttcgc agacagaggc acaacaactg gtggcggagg aactggtgga
     1981 aaagtgggaa aaaggaaaga tgcgtctgtt gtgggataac aaaaagcggc gtaacgaagc
     2041 gctggactgc ctggtgtatg cctatgcggc attacgtgtg tccgtgcaac gctggcaact
     2101 tgatctggct gtgctggcgg catcccgcaa ggatgcaact gcacaaccga cccttgaaga
     2161 actggcagcg aagctgtccg gaggagtgaa tggttacagt cgctgaactg caggcgctgc
     2221 gtcaggcgcg ccttgattta ttaaccggta aacgggttgt gtcggtacag aaagacggtc
     2281 gcaggatcga atatacggca gcgtccctga atgagcttaa ccgtgcgatc aacgatgcgg
     2341 agtcggttct gggtacgacc cgacgccgcc gaccgctggg agtgaggtta tgaaacgaac
     2401 gcctgtcctg attgatgtga acggcgttcc gcttcgtgag agtctcagct acaacggagg
     2461 cggcgcagga tttggcgggc aaatggcaga gtggttacca ccggcgcaga gtgccgatgc
     2521 ggccctgttg cctgcattgc gtctggggaa tgcccgggca gatgatctgg tgcgcaataa
     2581 cggtatcgcg gctaatgcgg tggccctgca taaggatcat atcgtcgggc acatgtttct
     2641 tatcagctac cgtctgaact ggcgttggct ggggatgcgg gagaccacgg caaaaagttt
     2701 tgtcggggag gtggaagcag cctggtcgga atacgcagaa ggaatgtttg gcgagatcga
     2761 cgtggaaggg aaacgcacgt ttacggaatt tatccgtgaa ggggtgggcg tgcatgcctt
     2821 taacggcgaa atctttgtgc agccggtctg ggatacggaa accacgcagt tattccgtac
     2881 gcgttttaaa gccgtgagtc cgaaacgggt ggacacgcca ggacacggta tggggaacca
     2941 ctttctgcgg gccggggttg aggtcgatcg gtatgggcgg gcgattgcgt accatatctg
     3001 tgaggatgat tttccgttct ctggtgccgg acgctgggag cggatcccgc gtgagcttcc
     3061 caccgggcgt ccggccatgc tgcatatttt tgagccggtg gaggacgggc agacccgtgg
     3121 ggccaatcag ttttacagcg taatggagcg gatgaagatg ctggactccc tgcaggccac
     3181 gcagcttcag tcggccattg tgaaggcgat gtatgcagcc acaatcgaaa gtgaacttga
     3241 taccgaaaag gcatttgaat atatcgcggg ggcaccgcaa ggtcagcagg ataatccgct
     3301 tattaatatt ctggagaagt tcacccgctg gtatgacacg aataacgtga cgctgggcgg
     3361 tgttaaaatt ccgcaccttt tttcctggcg atgcgctgaa cctgcagaca gcgcaggatt
     3421 cagacaatgg attttcggcg cttgaacagg cgctgctgcg gtatattgca gccggtcttg
     3481 gcgtttccta cgaacagttg tcccgtgatt actcgaaggt cagttactca agtgcccgtg
     3541 cctccgccaa tgagtcgtgg cgctatttta tggggcggcg aaaatttatt gcggcccggc
     3601 tggccacgca gatgttttcc tgctggctgg aagaggcgct tcttcggggg attattcgtc
     3661 cgccacgggc gcgttttgat ttttaccagg cgcgttcagc ctggtcacgg gcagagtgga
     3721 ttggctccgg aagaatggcc attgacgggc tcaaggaggt ccaggaatcg gtgatgcgca
     3781 ttgaggccgg actgagcacg tatgagaaag aactggcact gatgggcgaa gattatcagg
     3841 acattttccg ccagcaggtc agggaatccg cagagcgcga aaaagccgga ctctcacgtc
     3901 cggtgtggat agcccaggcg tatcagcagc agatagcgga gagtcgcagg ccggaagagg
     3961 agacaacatc acgtgagacg taatctttca catattatcg ctgcggcatt caatgagccg
     4021 ctgcttctgg agcccgccta tgcgcgggtt ttcttttgcg cgctcgggcg tgagatgggc
     4081 gcagcaagcc tttcggtacc gcaacagcag gtacagcttg acgctcccgg gatgctggct
     4141 gaaacggacg agtacatggc cggaggcaaa cgacctgccc gtgtttaccg ggtggtgaac
     4201 ggtattgctg tactgccggt gaccggcacg ctggtacacc ggctgggtgg catgcggcca
     4261 ttttccggaa tgactggcta tgacggcatt gtcgcctgtc ttcagcaggc aatggcggac
     4321 actcaggtac ggggcgtact gctggacatt gacagtcctg gcgggcaggc caccggcgcg
     4381 tttgactgcg ctgacatgat ttaccgtctc cggcagctga agcctgtctg ggcactgtgc
     4441 aatgacacgg cctgttcagc cgccatgtta ctggcatcgg cctgctcccg acggctggtt
     4501 acccagacat cccgtatcgg ctccattggg gtgatgatgg gccatgtcag ctatgccggt
     4561 catctggcac aggccggtgt ggatatcacg ctgatttacg ccggatcgca caaggtggat
     4621 ggcaatcagt ttgaatccct gccagcaaag gttcgccagg acatgcagca gcgcattgat
     4681 gcggcgcgcc ggatgtttgc tgaaaaagtg gcgatgtata ccggtttatc tgtggatgcg
     4741 gttatgggaa cagaggccgc cgtttttgaa ggtcagtccg gtattgaggc cgaactggcg
     4801 gatgaattaa tcaatgcgtc ggacgccatc agcgtgatgg ctgcgacgct gaacacacac
     4861 gatacaggag gcactatgcc gcaattaact gcaacggagg ctgccgcgca ggagaaccag
     4921 cgagtgatgg ggatcctgac gtgtcaggaa gcgaaaggac gtgaacaact ggccacgatg
     4981 ctggcagggc aacagggtat gagcgttgag caggcccggg cgattctggc cgtggcggca
     5041 ccgcaacaat ctggtgtatc tgcgcagagt gaagccgatc gcattatggc atgtgaagag
     5101 gcgaaaggac gtgaacaact ggcggcaacg ctggcggcga tgccggagat gacggtggaa
     5161 aaagcccgtc cgattctggc tgcttcaccg caggcgaatg ccggaccctc acttcgtgat
     5221 cagattatgg ccctggatga ggcaaaaggg gcagaggcgc aggctgaaaa actggcggcc
     5281 tgtccgggga tgaccgtgga gaatgcccgg gctgtactgg cggcagcctc aggcagggca
     5341 gaaccggttt ctgcatccac aaccgccatg tttgaacatt tcatggcgaa ccattcaccg
     5401 gcagcggtac agggtggcgt gccacagacg tcagcagacg gtgatgcgga cgtgaaaatg
     5461 ctcatggcca tgccatgaag tcagtgctga ccatcaatat gaggttttta caatatggta
     5521 acgaaaacca tcactgaaca gcgtgcggaa gtacgtattt ttgccggtaa tgatccggcc
     5581 cataccgcca caggcagcag cgggatttcc tcggcaacac cggctctgac gcccctgatg
     5641 ctggatgagg ataccgggaa actggtggtc tgggacggac agaaggcagg ccatgtggtt
     5701 ggcatactgg tactgccgct tgaaggtaca gagacggtgc tgacgtatta caagtcgggg
     5761 acctttgcga cggaggccat ccactggcct gagagagtgg atacacacaa aaaggcgaat
     5821 gcctttgccg gcagtgccct gagtcacgca gcgctgccgt aacattttat caggtcactg
     5881 cggtagcctg actgatttct gaatgaaagg aactgattta tgggattgtt tacgacccgc
     5941 cagttactcg gttataccga acaaaaagtt aaatttcgtg cgctgtttct ggagctgttt
     6001 ttccgccgta cggtgaattt ccataccgaa gaggtaatgc tggacaaaat taccggaaaa
     6061 acgccggtgg cggcctatgt ctccccggtt gttgaaggaa aggtgttgcg tcaccgtggc
     6121 ggtgaaactc gtgtactgcg cccgggctac gttaagccga aacacgaatt taattaccag
     6181 caggcggtgg agcgtcttcc cggtgaagat ccggcccagc tgaacgaccc ggcttaccgt
     6241 cgcctgcgta tcatcaccga taacctcaaa caggaagagc acgccattgt ccaggtggaa
     6301 gagatgcagg cggtgaatgc cgtgctgtat ggcaaataca ccatggaagg agagcagttt
     6361 gaaactgttg aggtcgattt tggccggtcg gtgggaaata acattacgca ggcaggaggg
     6421 acggagtgga gcgctcagga ccgcgatacc tttgatccga cgcatgatct ggatgcgtac
     6481 tgtgattttg cttccggcac catcaatatt gccattatgg atggtaaggt ctggcgtctg
     6541 ctgaatggct ttaagctgtt ccgcgaaaaa ctggataccc gtcgtggctc aaattcacag
     6601 ctggaaacgg cagtgaaaga cctgggggcg gtggtgtcct tcaaagggta ttatggtgat
     6661 ctggcaatcg tggtggcaaa aacgtcctat gtggcagagg acggtaccga aaaacgttat
     6721 ctgccggacg gcacactggt actgggaaat acggccgcag agggtattcg ttgctacggt
     6781 gccattcagg atgcgcaggc attgtctgaa ggcgtggtgg cgtcttcccg ttatccgaaa
     6841 cactggctga ccgtgggtga tccggcgcgt gaattcacca tgacgcagtc cgcgccgctg
     6901 atggtgctgc cggatccgga tgagtttgtg gtggtgcagg tgaaataatc cgtgagcggg
     6961 agctaaatgc tcccgttttt cattcaatca ggagcgggta tggctacgaa agaagaaaac
     7021 ctgaaacgtc tttgtgagct ggctgagagg ctggggcgtg aaccggatgt ctccggtagt
     7081 gcggcagata ttgcgcagcg agtggcggaa ctggaggaag agcttggaga tgcgggggag
     7141 gtcgcagagc cggatacgta ctcaccatcg aaagatgatc cgaccgcgca taaaggcgag
     7201 tcagttcctg aacagccagg cattgttacc ggagacgaaa cggggcaggt tacggtggtg
     7261 gcactggcga cactgcatac aaaatgtaat ggttcagtga tatttgtttc accgggaacg
     7321 tcgtttcgtg tttctgctga ggtggctgcc agcatggcgg caggtggtct ggcaaaaagg
     7381 cagtaacagg agatgtcgtg gacggtttcg ataatctgtt tgatgctgcg cttgccggtg
     7441 tcaatgaagt cattctccgt gatatgggga tcagcgccgt cattacgtcc ggagaacttg
     7501 aggggacaca cctgacaggg gtttttgatg atcccgaaag tatttctttt gttgccgggg
     7561 ggatccggct tgaggattcc tcaccgtgcc tgtttgtgaa aacggcagat atatcgcagt
     7621 tacgccgtca ggatacgtta actatcgggg atgattcttt ctttgtggat cgtattactc
     7681 cggatgacgg gggatgctgt tatatccggt taaggcgggg cagtcctgcc ccacagaata
     7741 atgccagaat gaggcgatat gatgaaggga cttgagaatg ccatccgcaa tctgaacagc
     7801 ctcgataccc gcatggtgcc acagaccagc gcatgggcgg taaaccgtgt ggcggcaaag
     7861 attgtttctg tcgccacacg gcaggttgcg cagaataccg ttgccgggga taaccaggtg
     7921 aaaggtattc ccctgaaaac ggtcagggag cgtgtcaggc tgcttaaagc cagcccctca
     7981 ggaaaaatgt atgcccgcat gcgtgttaac cggggtaacc tgcccgccat taagctgggt
     8041 accgcacagg tcagactggc ccgctcccgg catggcagca actcacgtca tcgtggcagc
     8101 gtactgaaag tggggaaata ccttttccgg gatgcgttta ttcagcaact ggcgaatggt
     8161 cgctggcatg tgatgcggcg tattgacggc aaaaatcgtt accccattga
//
LOCUS       DAEEGA010000138         8171 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEEGA010000138 DAEEGA010000000
VERSION     DAEEGA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.138"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(207..398)
                     /locus_tag="KLH46_003705"
                     /pseudo
     CDS             complement(207..398)
                     /locus_tag="KLH46_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            396..720
                     /locus_tag="KLH46_003706"
                     /pseudo
     CDS             396..720
                     /locus_tag="KLH46_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(750..3662)
                     /locus_tag="KLH46_003707"
     CDS             complement(750..3662)
                     /locus_tag="KLH46_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380237.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3665..3781
                     /locus_tag="KLH46_003708"
     CDS             3665..3781
                     /locus_tag="KLH46_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBE0380238.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            4145..4987
                     /locus_tag="KLH46_003709"
     CDS             4145..4987
                     /locus_tag="KLH46_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBE0380239.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4990..6078
                     /gene="capU"
                     /locus_tag="KLH46_003710"
     CDS             4990..6078
                     /gene="capU"
                     /locus_tag="KLH46_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBE0380240.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            6083..7033
                     /gene="virK"
                     /locus_tag="KLH46_003711"
     CDS             6083..7033
                     /gene="virK"
                     /locus_tag="KLH46_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HBE0380241.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            7098..8042
                     /gene="lpxM"
                     /locus_tag="KLH46_003712"
                     /gene_synonym="msbB"
     CDS             7098..8042
                     /gene="lpxM"
                     /locus_tag="KLH46_003712"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBE0380242.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2202 a         1840 c         1947 g         2182 t
ORIGIN      
        1 ggccacatcc ctccggcaga agcagaaaaa gcttattatg cttccatcgg aaacgatgat
       61 ctggcagcct gagttcacag ataaaacact ctccaggaaa cccggggcgg ttcaaagggc
      121 cttcccgaga gtggtaaagg agaggccctg gaaatacccc acagccccga aaaagagcca
      181 gtcagttgct taactgactg gcattaaggc cgccggggac gtttttttac caccgggagt
      241 ttgggcataa cggtgctttc agtaagtacg acaggtactg tggaggcact tccgtaaaga
      301 agggcatcaa gctcctgctc cgacatacct gcaggcaaag gccatgaaag ccctgatttc
      361 cggaagcgag cgaacagtcc acaaactgtt gaacgtggtg tcagcggcgg acagggataa
      421 gccctcgtca gcagttccgt cagcactgcg acagcgttct ttacccggtc aaaacagcgt
      481 tgcggtgcac cacgcctgac ggatgaactg cgtgctcagg gttaccactt taacgtaaaa
      541 accgtggcgg caagcctgcg ccgtcagggg ctgagggcaa aggcctcccg gaagttcagc
      601 ccggtcagtt accgcgcaca tggcctgccc gtgtcagaaa atctgctgga gcaggatttt
      661 tacgccagtg gtccgaacca gaagtgggcg ggagacatca cgtacttacg tacagatgaa
      721 gttcggttac atcctgtcag cacggaacct cacgcttttt gagcggcacg acggatcccc
      781 cccacccagt atccgacaga cttttcactg cgttcgtaaa aacccttctt ccctcccagc
      841 atatccacta attccggtga catatcctgc agataagtct gcaaatcatg ccccagatct
      901 tccggtgact gcaactcata aaccgcgcgc cacacatcac ccttccttat tttaagcccc
      961 gggttaagaa tatactgtcc gtggttcagc ctcagaaaaa gcttttttcc ataaggattt
     1021 ttgctgttaa cttcatgttg agagagctgg ctgctgatat aactccttcg tttgcggcgt
     1081 tccggcagca acgcttcagg aagatgaacc acacactctg ccagtcgtgc cgcactgaag
     1141 gcttccgaaa aaaacagagc acttcccaga tgcgtgtgga acagcgcctg catcaggatc
     1201 accagaaaca ttgccatctg ccggttgtcc agtttgatga ggcgtccttc cacctgcagt
     1261 gacagacttt caggtttcag cagggtatac aactgcgcac ttttctgttg cgcataacgg
     1321 ggcgtactga ccgccgctga cagaacttga tgataagccg ccagcccgtc attaccggtc
     1381 agcaagggat ctgcaccacg ctccagcagt aactgaacca gtgcaatatt acctgcccgg
     1441 gctgctgaca tcagcggagt aagattaaac ggcgaacggt gatcaacacc ataacgttca
     1501 atatcccgca acacggcaac cgggttattc attgagtatg gccggaaata cttcagctcc
     1561 agtaactgca gagtcttcgt tctgggctgc cttgtgggct taaaatcttc ccggtacagt
     1621 gcattataca gcgggaacag ggaataaatc agactgtact ccagcgcctg cagttgtatg
     1681 gttttatcgg ctgtacctgc gaggatatgc tgtttccact gtcgtgcatc ctctgcggtg
     1741 ataatctgcc atggcatctc agatgtctgc aggatccggc tacggatggt gtccgcctgc
     1801 tcctgcttcc cctgtttttc aagcagatgt gcggttttct gccactcatc acggctggaa
     1861 atctcttcct gcagattcag aggctcttcc tgatgcgtga gcgcaagcag actccacaga
     1921 ggatgctcca cctgctgttc aacaagatac acattgtgtg tagcacgggt gagcgcaaca
     1981 tacagcgcgt tggtaaaaaa tttatagatt tccgctgaac gatcctgcct gtcccgcgga
     2041 cgggaataac gggcctcgcc ctcaagatca gccggtgtaa gaccctcgca aatatcatca
     2101 aatgcctgcc gggcagcgct tactatattg taaaggatca ccgtttcgta ttccagcccc
     2161 ttaacctcct gaaccgaaaa gaccagcggc gtactgaacc agcatcgcgc ctgtgctttc
     2221 tgttccggat gcatcacaat gaccgctaca cgattcgaaa gtgaggtttt ggcattcagt
     2281 tcctgctttg tctcttccct atcgtccagc aaccggatgg tcccttctgc ctgaccgcag
     2341 cttctgacaa aatggtgact ttcccgatca atagcactaa agcggacctg tttgagtctc
     2401 agcaaccggt tagccagtgc agtgacgtgg tgcccgttac ggtaattcgc ctgcaggata
     2461 ttaacggttg tgtcgtttcc ctgttgctgc cggaaaaaga gacttttgag cgaactccat
     2521 gagaaaaagc tcggatgaac tatctgattg gcatcaccac agagtaaaaa ctgaccggga
     2581 tgacgtagcg ttttcagtac cagttgcagt tgaacaggcg tcatatcctg aacttcatca
     2641 acaaaaatga catcataacg gggagttgcc aacgaaagat actgatgact gagcagatta
     2701 ctgtcatact gatgcgactg tttcagccat gcgatataac gttcaaacag gacatagacc
     2761 gtcgggcggt cctccatgcc atagagcgac tgccgtatcc ccagtgacag atacgcctcc
     2821 cgggacagcg gcccattccc ggaggcaacc gcaccgatca cgcccctgaa ctcctcatac
     2881 agggtatggg cagcccccag tgcggcgata gccctgttgc ggggcagcca gtcgctgaac
     2941 gcactgaaag tcacctccct gccttccgga atgcgcagag tttccagaaa ttccgtcagc
     3001 gaaagaaagt caatattctg tacctcactc ccttcccctg aagcgtcata gagagtacgc
     3061 gctttttcca ccagaaatga ggaaaggctg agatacagta tatcaccagc agcctgcttc
     3121 attttttcca gtaccagcgc cgtcttacca cttcccgccg agccaacaat caccagcggt
     3181 agcgggtgtt cgtacagtgc ctgctgatca tcatcaaagg acagcatttt atccaggcgg
     3241 tggaaacgcc catgagacgg attgatatac gtcagcgctt cagtcgcgat atcaacagga
     3301 tccggcactg gttgttgttg taagcgccct tcatcaatca ccacgtttct gcgaagaaaa
     3361 cgtgaagtgt tataggcatg attgcgaata tattccagaa caaggcagat agtttcaccg
     3421 tggtagcgat acagcgaaaa cagtaatcgg tcccggatac tgagccgggc gcggtaaaga
     3481 ttggtgtcaa ttttacgaac atcagcctga acaaagttat cggtggcaat agcctgtgca
     3541 aattttttat aacccgggat cgtgtcagga ttcagagtat gataaagaca gattttcatg
     3601 gctgccggtt tccgtcgtgc aaaaaacatg ctgattttac gaaggaatgc cttactgaac
     3661 aaccttgccc ggaatacccc ccgaaatcag atggaacaat cagaaaaatg ctctgcagtt
     3721 atggaataaa gaaggtgggg tggtacgccg ggagggcgat ccgctggaaa tggcgcagta
     3781 gctgcaggcc tgtcatgatg ccggaataga agttcgtgta caggttaatg aaagcgtcac
     3841 cccgtaaata aactctgcgc tgaaattcct ttttttcatt ggagcacctg tgttgttctg
     3901 aggtgaacat atcacctctg ttcaggtagc caaattcagt gtgccacctc aaggttctta
     3961 gtgagtcgtt tttaaaagat atgataatat atcattgttt atgaattgtt gatgtttaat
     4021 ttagtaaatt atatcaaata tgccatgggt ttgctatgtt attcatccag tatctgggaa
     4081 taaaatgcac cagctttatt gggttttcct tttgtctctg cttactaggt gccagaaact
     4141 ttaaatgtta aacgaagggg gcattttgtt caaagccaat catgttccgg tattaatgta
     4201 tcatcatgtc agtcattgcc ccggactggt tacactttct cccgtgacat tccggaagca
     4261 aataaaatgg ttggctgaaa acaactggaa aacactgtca tcagatgaac tggagttttt
     4321 ttatcggggc ggaaaactcc cccggaaaag cgttatgttg acatttgatg atggatacct
     4381 tgacaactgg tttcaggtat atcctttatt aaaagagttt aatctcaaag cacatatttt
     4441 tttaattacc ggttttattg ggaatggtcc ggttcgtcac tctcctggaa aggaatattc
     4501 tcatcgcgat tgcgaacatc aaatagcgac agggaatgct gataatgtta tgcttcgctg
     4561 gtctgaagta aacgaaatgt tgcaatctgg tctggtcgaa tttcatgttc atacgcatac
     4621 tcatacacgc tgggataaaa agttcagttc acgagaagaa cagtgtaaac atcttcggca
     4681 ggacttgttg agcgggagag aatatttgaa agaaatgaca ggaaaatgca gtaaacattt
     4741 gtgttggccg gaaggttatt ataacaagga ttatattcag gtcgctgaag aacttggatt
     4801 ctattattta tatacaacag aaagaagaat gaatgctcct gctaaaggaa ctacacggat
     4861 aggaagaata agcaccaaag aacgggaaag ttgcgcatgg ctgaaaagac gactttttta
     4921 ttatacaacc ccgttttttt catcgttgct ggcctttcat aaggggccaa gattaccgga
     4981 tgattaataa tgaatatact atttacggaa tcatcaccga atataggtgg tcaggaatta
     5041 caggctgttg ctcaaatgaa ggccctgaag aaaatggggc attcagttct gcttgtctgc
     5101 agggaaaaca gcaaaattgc ttttgaagcc agtaaattgg ggattgatat cacattcgcg
     5161 ttatttcgaa acagtcttca catccctact gcatggagat tactcggaat agttcatggt
     5221 tttcagccca atgcaatcgt ttgtcacagt gggcatgata gcaatattgt tggtttagta
     5281 cggttattca ctaggaaaca tccattcaga attatcaggc aaaagacata tttgacacga
     5341 aaaacaaaag ttttttcaat aaatcatttt tgcgatgagg tgattgttcc cggaacaagt
     5401 atgaagacac atctggagca ggaaggatgt cgaacccggg ttactgttgt gcctccaggc
     5461 tttgatttcc agaaattata cgttgattcc cgaaacagtt tgcctccaaa tgttctttct
     5521 tggctggcgt cccgaagggg atgccccgtt attgctcagg taggaatgtt gcgcccggaa
     5581 aaagggcacg aatttatgtt gaatttactg ttccatttaa aaatgaatgg acgacagttc
     5641 tgttggttga ttgtggggtc tggttcgcct gaactgcggg agcatttaca gtatcagatt
     5701 gacagtatgg gcatgcatga tgatgttttt attgctgaca atgtttttcc tgccgccccc
     5761 gtatatcggg ttgccagtct ggtggttctg ccttcagaaa acgaatcgtt tggtatggta
     5821 ctggcagaag catcggcatt ttctgtgcct gtactggcca gtcagattgg tggaatccct
     5881 gatgttattc agaataacca gaccgggaca ttgttaccag caggtaataa gcacgcatgg
     5941 atgtgcgccc tgaatgattt ttttaatgac cctgggcgtt tttatcagat ggctcgccag
     6001 gcaaaacagg atatagaaga gcggtttgat attaataaaa ctgcgttaaa aatactcaca
     6061 ttagcgaagc acaagtaaca atatgttttc tgtaagtaac ttatcattta tcggtttcct
     6121 taaaaggatt gttttttcct cagattcact cccggggaag tgggaacaca gaaaattccg
     6181 gttcatgtac attttgcgat gtgctataaa tccggttgcc agtattcgat attattacga
     6241 actgcgttcc ttgcagtgca ttgaggatat tctggcaata caacccacgt tgccagcaag
     6301 aattcatcga ccttatttgc ataaaggggg gcgagcctgg tcccgggggc aatatattct
     6361 tgagcattat cgctttgtgc aaaatttgcc agaaaaatat tctgaatttt tgtttccaca
     6421 aaaatcggta tcattagtcc aattcatcgg gaaagatgga gaggattttg atatccagtg
     6481 ttctcccagt ggttttgacc gggaaggaga gttgatgttg tcattgtttt tcaataaaat
     6541 agtgattgcc cggttaacat tttcagtcat tctgactcaa aatggacaca ccgcttttat
     6601 tggcggatta cagggagccc ccaaaaatac cggacctgat gttattcggt gcgcaacccg
     6661 ggcctgttat gggcttttcc caaaacggat tatttttgaa gcattttgtg cgttgatgaa
     6721 agcttgtaat gtctctgagt gtctggcagt cagtgagcac agtcatgttt tccggcaatt
     6781 gagatactgg tatcagaaac gcaaaacctt tgttgctgtc tacagtgatt tctgggagtc
     6841 tgtagcaggg aaaacctgtg gtgactggta caaattacca acacaggtgg ttcgaaagcc
     6901 gctaagcaat atagctagta aaaaacgctc tgagtatcga aaacgatacg ctttgctgga
     6961 ttatatccat gaaactgcta tccgctcttt ggatgcatat cctgtaaact cagaacatca
     7021 ggacttaaat taaggtttga tgtattctct gaataaaata ttaatgatga ttttggtagg
     7081 ggcattcgca ctaaataatg aaaaaataca aatccgagtt tattcctgaa tttaagaaaa
     7141 attatctttc ccctgtttac tggtttacat ggttcgtttt gggaatgatt gcaggtattt
     7201 caatgtttcc cccttcattc agagatcctg tcttggccaa aatagggcgt tgggtgggca
     7261 gattgagcag aaaagctcgt cgcagggcga cgattaattt atcgctttgt ttcccggaaa
     7321 agagtgatac agaacgggaa ataattgtcg acaatatgtt tgccacagca ttgcaatcca
     7381 tagtgatgat ggcagaactg gcaattcgtg gtccggaaaa gttccagaaa cgagtgttct
     7441 ggaaaggcct cgaaattctt gaggaaattc ggcacaataa cagaaatgtg atttttctgg
     7501 ttccccatgg ctggagcgtg gatattcctg caatgttgct ggcagctcag ggggaaaaaa
     7561 tggccgccat gtttcatcag caacgaaatc cagtgattga ttatgtctgg aattctgtac
     7621 ggcgtaaatt cggggggcgc ttacattccc gggaggatgg gataaaacca tttattcagt
     7681 cagtacgcca gggatactgg gggtattacc ttccagatca ggatcatggt cctgaataca
     7741 gtgaatttgc tgattttttt gcgacctata aagcgacatt accaattatt ggacgtctga
     7801 tgaacatcag tcaggctatg attataccgc ttttcccggt ttatgatgaa aaaaaacatt
     7861 tcctgactat tgaagttcgg ccaccaatgg atgcatgcat tgccagcgcg gacaataaaa
     7921 tgattgcccg acaaatgaac aaaacagtgg aaattttggt ggggtcacat ccggaacagt
     7981 atatctgggt tttaaaattg ttaaaaacgc gcaaatcaaa cgaagcggac ccgtaccctt
     8041 gaaattataa agtacaggta tttccactag ttgtttctta caggttacca atcgaaacac
     8101 atcccccttc cgtgtgttgc ccgtttggca cggaggactg tgtagatgca gttcgttaaa
     8161 tttaatttat g
//
LOCUS       DAEEGA010000139         8050 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEEGA010000139 DAEEGA010000000
VERSION     DAEEGA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.139"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            55..588
                     /gene="ymdB"
                     /locus_tag="KLH46_003713"
     CDS             55..588
                     /gene="ymdB"
                     /locus_tag="KLH46_003713"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBE0380243.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            530..2011
                     /gene="clsC"
                     /locus_tag="KLH46_003714"
     CDS             530..2011
                     /gene="clsC"
                     /locus_tag="KLH46_003714"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HBE0380244.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2019..3176)
                     /gene="mdoC"
                     /locus_tag="KLH46_003715"
     CDS             complement(2019..3176)
                     /gene="mdoC"
                     /locus_tag="KLH46_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBE0380245.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3570..5105
                     /gene="mdoG"
                     /locus_tag="KLH46_003716"
     CDS             3570..5105
                     /gene="mdoG"
                     /locus_tag="KLH46_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBE0380246.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5098..7641
                     /gene="mdoH"
                     /locus_tag="KLH46_003717"
     CDS             5098..7641
                     /gene="mdoH"
                     /locus_tag="KLH46_003717"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBE0380247.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1998 a         1930 c         2113 g         2009 t
ORIGIN      
        1 ttaaccaaaa agcctgctgt acacttaaga aacaagaagg tgtaaggagg cgttatgaaa
       61 acgcgtattc atgttgtgca gggtgatatt accaaactgg ccgttgatgt gattgtgaat
      121 gcggctaatc cgtcattaat gggaggcggc ggcgtcgatg gggccattca tcgcgcagcg
      181 ggtccggccc tgctggatgc ctgtttaaaa gtcaggcaac agcagggcga ttgccctacg
      241 gggcatgccg ttattacgct tgcaggcgat cttcccgcta aagccgtagt acacaccgtc
      301 gggccagtct ggcgtggtgg ggaacaaaac gaagaccagc ttttgcagga tgcctatctg
      361 aatagcctac gactggtggc ggcaaacagc tatacgtcag tggcttttcc tgcaatcagt
      421 actggggttt atagttaccc tcgtgcggca gcggctgaaa tcgcagttaa aaccgtttca
      481 gaatttatta cccgtcacgc tttacccgaa caggtatact ttgtctgtta tgatgaagaa
      541 aacgcccacc tctacgaaag actccttacc caacaaggag atgaatgatt tgccccggct
      601 ggcgagcgcg gtgctgccac tgtgttcgca acatcccggt cagtgtggtc ttttccctct
      661 ggagaaaagt ctggatgcgt ttgccgcccg gtatcgtctg gccgaaatgg cagagcatac
      721 gctggatgtt cagtattaca tctggcagga cgatatgtcg gggcggttac tgttttccgc
      781 cctgttagcc gcagcaaagc gtggcgttcg cgtccgtttg ttgctggacg acaacaatac
      841 gcccggactt gacgacattt tgcgcttgct tgacagtcat ccacgcattg aagtccggct
      901 ttttaatcct ttctcgtttc gcttgctacg tccgcttgga tatatcaccg acttttcccg
      961 tctcaatcgc cggatgcaca ataaaagttt cactgtcgat ggcgtggtta ccctagtggg
     1021 aggacgaaat attggtgatg cctattttgg tgcaggggaa gagccacttt tttcggattt
     1081 agatgtcatg gcaataggcc ccgtggtaga ggatgttgcc gatgatttcg cccgctactg
     1141 gtattgcaaa tcggtttcac ccttacagca ggtgctggat gtcccggaag gtgaaatggc
     1201 ggatcgcatc gagttacccg cctcctggca taacgatgcc atgacgcatc gttatttacg
     1261 caaaatggaa tccagtccat ttataaatca tctggttgat ggaacgttgc cgcttatctg
     1321 ggcgaagaca cgtttattaa gtgatgatcc ggcgaaaggg gagggcaagg caaaacggca
     1381 ttcactgtta ccgcagcgcc tgttcgatat catgggctca cccagtgaac gcatcgatat
     1441 tatctcttcc tattttgtac cgacacgcgc aggtgtggcg caactcttac ggatggtgag
     1501 aaaaggggta aagattgcga tcctaaccaa ttctcttgcc gctaacgatg ttgctgtcgt
     1561 ccatgccgga tacgcgcgct ggcgcaaaaa attgctccgc tatggcgtgg aattatatga
     1621 actcaagccg acgcgtgaac aaagcagtac gttacacgat cgcggcataa ccggtaattc
     1681 cggtgccagc ctgcatgcta aaacttttag catcgatggt aaaacggtgt ttatcggctc
     1741 tttcaatttc gatccgcgtt caacattgct caatactgaa atgggcttcg tgatagagag
     1801 cgaaacgctg gcacagttaa ttgataaacg ctttattcag agccagtacg atgcggcctg
     1861 gcagctccgt ctggacaggt ggggacggat caactgggtt gatcgtcatg caaagaaaga
     1921 ggttattctc aaaaaagaac ccgccaccag tttctggaag cgggttatgg tcagactggc
     1981 gtcgatattg cccgtggaat ggttattgta aatgtggctt aacgggctgg tgctttatcg
     2041 ttctcacgct taacaaccgg tttaccagag aacaaaaact tcagtaacgg gatgcgtaaa
     2101 tgaatttcat acagaattat cgcaatccct actacgaata tcaggccaca gagaaaacca
     2161 agccagttgg aggtgatgtg cggtgtaatg tatgcgccga aaaacagcgt taacgggtgg
     2221 tgaaccagat agataaacag cgatgcgtta acaaaataag tcacccgcgc tgactggaag
     2281 ttaagcaaac ggtggccaaa ggagaagacc acattcacca tccacaggcc gaggaccatg
     2341 gtgatcaccg actcggtttc gtacatccag gcatcgccac tgccatagcg ctggttgagt
     2401 aaataagcga caaacgccaa tgctgcagcg agggtacagc cacgagacgg cgtggtaaac
     2461 aaggctttaa gatgagggaa aatgaaagcc agtgcgccga ggataaagaa cggtaaataa
     2521 aatagcgttt gcatgacaat aaaattgaac atgccattac tcagaatggg tggatacaca
     2581 ataaaaatcg ttcttcttat taccgcatag ccgatgccga ggcataaaaa aatcaccgat
     2641 agttttacca tcgagaattt tttattcgtt ttatcagaat tttctaaatt atttctgatg
     2701 cgcttaaata tccatacaca cagcgtcgtc atgactacta acaccagtaa aaaccacagg
     2761 tgtgatatta attcccaggc caacgtatta tatttgtcat acaatgatag cccaggccaa
     2821 ctttccgctt tccctttgac atattgcagc ataataaatt gcggtaatgt cagtaggggg
     2881 atggctgtta acatcgggat acctacacgt tcgacacgta ctttccacca ttttttcaag
     2941 ggatagcgta aaaaaagcat gtaggaaaag tatccggata taacgaaaaa tacctgcatg
     3001 cggaacgagt ggatgaagtc attaaaaagg gttagccaca atgacggttc ggcgctattc
     3061 acatgccatg tatggctcga atagattaaa gaaatatgaa aagggatccc taacaacatc
     3121 agccaggcgc ggatggagtc gaggaaatat tcacgttgcg cgggtactgg attcatatat
     3181 ggttaactaa tctcggattt ttcgtcttat ccctgtcggg ttatgccttt aggcttgttg
     3241 ccatagcgac accgacctga ccgcgccagg cgcaggcttc aaggttttta tgcatagcat
     3301 catcgctacc actaaccaga atggaagcgt ctgtaagacg gttgataaat aaatttgctg
     3361 gcaaacccta cacgaagtcg atgcttctgt ctttaggaga agcacggaaa gtgaaaacgg
     3421 ttgcaatcag gtgcttaatc catgagccag cgtgctgaac gataccggga ttcagttgtc
     3481 ggaatggctg gttatccatt aaaatagatc ggatcgatat aagcacacaa agggggaagt
     3541 gcttactaat tatgaaacat aaactacaaa tgatgaaaat gcgttggttg agtgctgcag
     3601 taatgttaac cctgtataca tcttcaagct gggctttcag tattgatgat gtcgcaaagc
     3661 aagctcaatc cttagccggg aaaggctatg aggcgcctaa aagcaacttg ccctccgttt
     3721 tccgcgacat gaaatacgcg gactatcagc agatccagtt taatcatgac aaagcgtact
     3781 ggaacaatct gaagacccca ttcaaactcg agttctacca tcagggtatg tacttcgata
     3841 ccccggtcaa aataaatgaa gtgactgcca ccgcagtcaa acgaatcaaa tacagcccgg
     3901 attatttcac tttcggcgat gttcagcatg acaaagacac ggtaaaagac cttggttttg
     3961 ccggttttaa agtgctttac ccgatcaaca gcaaagataa aaacgatgaa atcgtcagca
     4021 tgctcggggc cagctatttc cgcgtgattg gtgcaggtca ggtttatggc ctttctgccc
     4081 gcggcctagc aattgatacc gctttgccat cgggtgaaga atttccacgc ttcaaagagt
     4141 tctggatcga gcgtccaaaa ccgactgata aacgtttaac catttatgca ttgcttgact
     4201 cgccgcgtgc gacaggtgct tacaaattcg tggttatgcc agggcgtgac acggttgtgg
     4261 atgtgcagtc gaaaatctat ctgcgcgata aagtcggcaa actgggggtt gcaccgttaa
     4321 ccagtatgtt cctgtttggg ccgaaccaac cgtcgcctgc aaataactat cgtccggagt
     4381 tgcacgactc taacggtctg tctatccatg ctggtaatgg cgaatggatc tggcgtccgt
     4441 tgaataaccc gaaacattta gcggtcagca gcttctccat ggaaaacccg cgaggctttg
     4501 gtctgttgca acgaggtcgt gatttctccc gctttgaaga tctcgatgat cgttacgatc
     4561 tccgtccaag cgcatgggtg actccgaaag gggagtgggg caaaggcagc gttgagctgg
     4621 tggaaattcc aaccaacgat gaaaccaacg ataacatcgt cgcttactgg acgccggatc
     4681 agctgccgga gccgggtaaa gagatgaact ttaaatacac catcaccttc agccgtgatg
     4741 aagacaaact gcatgcgcca gataacgcat gggtgcaaca aacgcgtcgt tcaacggggg
     4801 atgtgaagca gtcgaacctg attcgccagc ctgacggtac tatcgccttt gtggtcgatt
     4861 ttaccggcgc agagatgaaa aaactgccag aggatacccc ggtcacagcg caaaccagca
     4921 ttggtgataa tggtgagata gttgaaagca cggtgcgcta taacccggtt accaaaggct
     4981 ggcgtctggt gatgcgtgtg aaagtgaaag atgccaagaa aatcactgaa atgcgtgctg
     5041 cgctggtgaa tgccgatcag acgttgagtg aaacctggag ctaccagtta cctgccaatg
     5101 aataagacaa ctgagtacat tgacgcaatg cccatcgccg caagcgagaa agcagcattg
     5161 ccgaagactg atatccgcgc cgttcatcag gcgctggatg ccgaacaccg cacctgggcg
     5221 cgggaggatg attccccgca aggctcggta aaggcgcgtc tggaacaagc ctggccagat
     5281 tcacttgctg atggacagtt aattaaagac gacgaagggc gcgatcagct taaggcgatg
     5341 ccagaagcaa aacgctcctc gatgtttccc gacccgtggc gtaccaaccc ggtaggccgt
     5401 ttctgggatc gcctgcgtgg acgcgatgtc acgccgcgct atctggctcg tttgaccaaa
     5461 gaagagcagg agagcgagca aaagtggcgt accgttggta ccatccgccg ttacattctg
     5521 ttgatcctga cgctcgcgca aactgttgtc gcgacctggt atatgaagac cattcttcct
     5581 tatcagggtt gggcgctgat taatcctatg gatatggttg gtcaggattt gtgggtttcc
     5641 tttatgcagc ttctgcctta tatgctgcaa accggtatcc tgatcctttt tgcggtactg
     5701 ttctgttggg tgtccgccgg attctggacg gcgttgatgg gcttcctgca actgcttatt
     5761 ggtcgcgata aatacagtat atctgcgtca acagttggcg atgaaccatt aaacccggag
     5821 catcgcacgg cgttgatcat gcctatctgt aacgaagacg tgaaccgtgt ttttgctggc
     5881 ttgcgtgcaa cgtgggaatc agtaaaagcc accgggaatg ccaaacactt tgatgtctac
     5941 attctgagtg acagttataa cccggatatc tgcgtcgcag agcaaaaagc ctggatggag
     6001 cttatcgctg aagtcggtgg cgaaggtcag attttctatc gccgccgccg tcgccgcgtg
     6061 aagcgtaaaa gcggtaatat cgatgacttc tgccgtcgct ggggcagcca gtacagctac
     6121 atggtggtgc tggatgctga ctcggtaatg accggtgatt gtttatgcgg cctggtgcgc
     6181 ttgatggaag ccaacccgaa cgccgggatc attcagtcgt cgccgaaagc gtccggtatg
     6241 gatacgctgt atgcgcgctg tcagcagttc gcgacccgcg tgtatgggcc actgtttaca
     6301 gccggtttgc acttctggca acttggcgag tcgcactact ggggacataa cgcgattatc
     6361 cgcgtgaaac catttatcga gcactgcgca ctggctccgc tgccgggcga aggttccttt
     6421 gccggttcaa tcctgtcaca tgacttcgta gaagcggcgt tgatgcgccg tgcaggttgg
     6481 ggggtctgga ttgcttacga tctcccgggt tcttatgaag aattgccgcc taacttgctt
     6541 gatgagctaa aacgtgaccg ccgctggtgc cacggtaacc tgatgaactt ccgtctgttc
     6601 ctggttaagg gtatgcaccc ggttcaccgt gcggtgttcc tgacgggcgt gatgtcttat
     6661 ctctccgctc cgctgtggtt tatgttcctc gcgctctcta ctgcattgca ggtagtgcat
     6721 gcgttgaccg aaccgcaata cttcctgcaa ccacggcagt tgttcccggt gtggccgcag
     6781 tggcgtcctg agctggcgat tgcacttttt gcttcgacca tggtgctgtt gttcctgccg
     6841 aagttattga gcattttgct tatctggtgc aaaggaacga aagaatacgg cggcttctgg
     6901 cgcgttacat tatcgttgct gctggaagtg ctgttttccg tgctgctggc tccggtacgc
     6961 atgctgttcc atacggtctt cgttgtcagc gcgttccttg gctgggaagt ggtgtggaat
     7021 tcaccgcagc gtgatgatga ctccacttca tggggcgaag cgttcaaacg ccacggctca
     7081 cagctgctgt tggggttagt gtgggctgtt gggatggcgt ggctggatct gcgtttcctg
     7141 ttctggctgg caccgattgt cttctcgttg atcctgtcac cgtttgtttc ggtgatttcc
     7201 agccgtgcca ccgttggtct gcgcaccaaa cgctggaaac tgttcctgat cccggaagag
     7261 tattcaccgc cgcaggtgct ggttgatacc gatcggttcc ttgagatgaa tcgccaacgc
     7321 tcccttgacg atggctttat gcacgcagtg tttaacccgt catttaacgc tctggcaacc
     7381 gcaatggcga cagcgcgtca ccgcgccagt aaggtgctgg aaatcgcccg tgaccgccac
     7441 gttgaacagg cgcttaacga gacgccagag aagctgaatc gcgatcgtcg cctggtgctg
     7501 ctaagcgatc cggtgacgat ggcccgtctg catttccgcg tctggaattc ccctgaaaga
     7561 tattcttcat gggtgagtta ttacgaaggg ataaagctca atccactggc attgcgtaaa
     7621 ccggatgcgg cttcgcaata aaaacgtagt tgcctgatgc gctacgctta tcaggcctac
     7681 atcgttcctg caatttattg attttgcaag attttgtagg tcggataagg cgttcacgcc
     7741 acatccggca aaaacaacga gccaataaaa ataccggcgt tatgccgtaa tgccagtcag
     7801 ttaagcaact gactggctct ttttcggggc tgtggggtat ttccagggcc tctcctttac
     7861 cactctcgat cgataggaat taaaacccca aaaagattaa aaaaacacca caaaacggat
     7921 gtttcttcaa caccactttt gctccatatg aacggaaccg acgattaaac tggatggctc
     7981 tgattgattc agggtatgaa tggcggtttt ttgctccgtt tccctcaaaa tggacgcaac
     8041 ttcccctctg
//
LOCUS       DAEEGA010000140         7916 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEEGA010000140 DAEEGA010000000
VERSION     DAEEGA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.140"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            237..740
                     /gene="dps"
                     /locus_tag="KLH46_003718"
                     /gene_synonym="pexB"
     CDS             237..740
                     /gene="dps"
                     /locus_tag="KLH46_003718"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBE0380248.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1144..1890
                     /gene="glnH"
                     /locus_tag="KLH46_003719"
     CDS             1144..1890
                     /gene="glnH"
                     /locus_tag="KLH46_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBE0380249.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            2029..2688
                     /gene="glnP"
                     /locus_tag="KLH46_003720"
     CDS             2029..2688
                     /gene="glnP"
                     /locus_tag="KLH46_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBE0380250.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2685..3407
                     /gene="glnQ"
                     /locus_tag="KLH46_003721"
     CDS             2685..3407
                     /gene="glnQ"
                     /locus_tag="KLH46_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBE0380251.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3668..5892
                     /gene="ybiO"
                     /locus_tag="KLH46_003722"
                     /pseudo
     CDS             3668..5892
                     /gene="ybiO"
                     /locus_tag="KLH46_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5889..6815)
                     /gene="rlmF"
                     /locus_tag="KLH46_003723"
     CDS             complement(5889..6815)
                     /gene="rlmF"
                     /locus_tag="KLH46_003723"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBE0380252.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7091..7351
                     /locus_tag="KLH46_003724"
     CDS             7091..7351
                     /locus_tag="KLH46_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE0380253.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7616..>7916
                     /locus_tag="KLH46_003725"
     CDS             7616..>7916
                     /locus_tag="KLH46_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBE0380254.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         1934 a         2025 c         1978 g         1979 t
ORIGIN      
        1 cgtatttata tatcgaatta tgcaaaagca tatttattcc gaaaattcct ggcgagcaga
       61 taaataagaa ttgttcttat caatatatct aactcattga atctttatta gttttgtttt
      121 tcacgcttgt taccactatt agtgtgatag gaacagccag aatagcggaa cacatagccg
      181 gtgctatact tagtctcgtt aattactggg acataacatc aagaggatat gaaattatga
      241 gtaccgctaa attagttaaa tcaaaagcga ccaatctgct ttatacccgc aacgatgtct
      301 ccgacagcga gaaaaaagca acagtagagt tgctgaatcg ccaggttatc cagtttattg
      361 atctttcttt gattaccaaa caagcgcact ggaacatgcg cggcgctaac ttcattgccg
      421 tacatgaaat gctggatggc ttccgcaccg cactgatcga tcatctggat accatggcag
      481 aacgtgccgt gcagctgggc ggtgtagctc tggggaccac tcaagttatc aacagcaaaa
      541 ccccgctgaa aagttacccg ctggacatcc acaacgttca ggatcacctg aaagaactgg
      601 ctgaccgtta cgcaatcgtc gctaatgacg tacgcaaagc gattggcgaa gcgaaagatg
      661 acgacaccgc agatatcctg accgccgcgt ctcgcgacct ggataaattc ctgtggttta
      721 tcgagtctaa catcgaataa atccatcgct gatggtgcag aactttagta cccgataaaa
      781 gcggcttcct gacaggaggc cgttttgttt tgcagcccac ctcaacgcac ttatttagtg
      841 catccatctg ctatccccag ctgattaagt aaattttttg tatccacatc atcacacaat
      901 cgttacataa agattgtttt ttcatcaggt tttacgctaa ataatcactg tgttgagtgc
      961 acaattttag cgcaccagat tggtgcccca gaatggtgca tcttcagggt attgccctat
     1021 aaatcgtgca tcacgttttt gccgcatctc gaaaaatcaa tgagttgcaa aattggcacg
     1081 attttttcat atatgtgaat gtcacgcagg ggatcgtccc gtggatagaa aaaaggaaat
     1141 gctatgaagt ctgtattaaa agtttcactg gctgcactga ccctggcttt tgcggtttct
     1201 tctcatgccg cggataaaaa attagttgtc gcgacggata ccgccttcgt tccgtttgaa
     1261 tttaaacagg gcgataaata tgtgggcttt gacgttgatc tgtgggctgc catcgctaaa
     1321 gagctgaagc tggattacga actgaagccg atggatttca gtgggatcat tccggcactg
     1381 caaaccaaaa acgtcgatct ggcgctggcg ggcattacca tcaccgacga gcgtaaaaaa
     1441 gcgatcgatt tctctgacgg ctactacaaa agcggcctgt tagtgatggt gaaagctaac
     1501 aataacgatg tgaaaagcgt gaaagatctc gacgggaaag tggttgctgt gaagagcggt
     1561 actggctccg ttgattacgc gaaagcaaac atcaaaacta aagatctgcg tcagttcccg
     1621 aacatcgata acgcctatat ggaactgggc accaaccgcg cagacgccgt tctgcacgat
     1681 acgccaaaca ttctgtactt catcaaaacc gccggtaacg gtcagttcaa agcggtaggt
     1741 gactctctgg aagcgcagca atacggtatt gcgttcccga aaggtagcga cgagctgcgt
     1801 gacaaagtca acggcgcgtt gaaaaccctg cgcgagaacg gaacttacaa cgaaatctac
     1861 aaaaaatggt tcagtactga accgaaataa taacgctaca cctgtaaagc gcactggcag
     1921 ttccctctcc cccctgggga gaggattagg gtgaggggcg caaacccgct ccggggccat
     1981 taattaccct gaatttgatt atttacacca cggtaacagg aacaacatat gcagtttgac
     2041 tggagtgcca tctggcctgc cattccgctt ctgattgaag gtgccaaaat gaccctgtgg
     2101 atttcggtcc tcggtctggc tggcggtctg gtaatcggat tgctggcagg ttttgcacgc
     2161 accttcggtg gttggatagc caaccacgtc gcgctggtct ttattgaagt gatccgcggc
     2221 acacctatcg tcgtccaggt gatgtttatc tatttcgccc tgccgatggc gtttaacgac
     2281 ttacgcatcg acccatttac tgcggcggtg gtcaccatca tgatcaactc cggcgcgtac
     2341 attgcggaaa tcacgcgtgg tgcggtgctg tctatccaca aaggttttcg tgaagcagga
     2401 ctggcgctcg gtctttcacg ttgggaaacc attcgctacg tcattttacc gctggcactg
     2461 cgtcgtatgc tgccgccgct gggtaaccag tggatcatca gcattaaaga cacctcgctg
     2521 tttattgtta tcggcgtggc ggaactgacc cgtcaggggc aagaaattat tgccggtaac
     2581 ttccgcgccc ttgagatctg gagcgccgtg gcggtgttct atctgattat taccctggtg
     2641 ctgagcttta ttctgcgtcg tctggaaaga aggatgaaaa tcctgtgatt gaatttaaaa
     2701 acgtctccaa gcactttggc ccaacccagg tgctgcacaa tatcgatttg aacattgccc
     2761 agggcgaagt cgtggtgatt atcgggccgt ccggttccgg taaatcgacc ctgctgcgct
     2821 gcatcaacaa actggaagaa atcacctccg gcgatctgat tgtcgatggc ctgaaggtca
     2881 acgatccgaa agttgacgag cgcctgattc gccaggaagc aggtatggtg ttccagcagt
     2941 tttacctctt cccgcatctg acggcgctgg gaaacgtcat gtttggtccg ctacgcgtgc
     3001 gtggcgcgaa caaagaagag gcggaaaaac tggcgcgtga gctgctggcg aaagtcggtc
     3061 tggcagaacg tgcacatcac tacccttccg aactttctgg tggtcaacag cagcgtgtgg
     3121 cgattgcccg cgcgctggcg gtgaagccga aaatgatgct gtttgatgaa ccgacttccg
     3181 ctcttgaccc ggaactgcgc catgaagtgc tgaaggttat gcaggatctg gctgaagaag
     3241 ggatgacgat ggtgatcgtg acccacgaaa tcggttttgc cgagaaagta gcttcgcggc
     3301 tgatctttat cgacaaaggc cggattgcgg aagatggcaa tccgcaggtg ttgatcaaga
     3361 acccgccgag ccagcgcttg caggaatttt tgcagcacgt ctcttaataa gacacattgc
     3421 ctgatgcgct acgcttatca ggcctacagg atatctggca acttattaaa attgcatgaa
     3481 cttgtaggac ggataaggcg ttcacgccgc atccggcaaa aaagcccgca cgttgtcagc
     3541 aacctgctta atatcccttc ctccctttca cccgaaaggg aggcacacca gattcctctc
     3601 atttaaaatc gcccctcccc cagcatctat acttatcttt ttgctctgtt ttctcactgg
     3661 aggagtcatg cggtggatcc tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc
     3721 ggtatccata cccggcgtta caaccacaac gacaacggac tcaacgactg aaccggcccc
     3781 ggaaccggat atcgaacaaa aaaagcggcc tatggcgcac tggcggatgt gctggataac
     3841 gacacctcgc gtaaagagtt gatcgaccag ttgcgtactg tcgccgcgac gccccctgcg
     3901 gaaccggtcc cgaagatcgt gccgccgacg ctggttgaag agcaaaccgt gctgcaaaag
     3961 gtcaccgaag tcagccgcca ttatggtgaa gccctttccg cccgcttcgg gcaactttat
     4021 cgcaatatca ccggctcccc gcataagcca tttaatccac aaaccttcag caatgcgctg
     4081 agccattttt caatgttagc ggtattagtg tttggttttt actggctgat tcgcctgtgc
     4141 gcactgccgc tatatcgcaa aatgggtcag tgggcgaggc aaaaaaatcg tgagcgcagc
     4201 aactggttgc agcttccggc gatgattatt ggggcgttta ttatcgacct gctgttactg
     4261 gccctgacat tatttgtcgg ccaggtatta agcgacaacc tgaatgcagg cagtcgcacc
     4321 atcgctttcc aacaaagttt gtttctcaac gcctttgccc tgattgaatt tttcaaagcc
     4381 gtactacgcc tgattttttg cccaaacgtg gcggagctgc gcccgttcac gattcaggac
     4441 gagagcgccc gttactgggg tcgtcgcctg agctggttaa gcagcctgat tggctatggc
     4501 ctgattgtgg ccgtgccgat tatctctaat caggtgaatg tacagatagg tgcgctggcg
     4561 aacgtcatca ttatgctgtg catgaccgtc tgggcgttgt acctgatctt ccgaaataaa
     4621 aaagagatta cccagcatat gctcaacttt gcggagcatt cgctggcttt tttcagcctg
     4681 tttatccgcg cctttgcgct ggtgtggcac tggctggcaa gcgcctattt tatcatgctg
     4741 tttttctttt cgttgttcga tccgggcaac agtctgaaat ttatgatggg tgcaacggtg
     4801 cgcagcctgg cgattattgg tatcgcagcg tttgtttccg gtatgttttc ccgctggctg
     4861 gcgaaaacca tcaccctctc gccacatact cagcgtaact atccggagct acaaaaacgg
     4921 ctgaatggct ggctttcggc ggcgctgaaa acggcgcgta ttctgaccgt ctgcgtggca
     4981 gtaatgttgc tgttgagcgc atggggattg ttcgatttct ggaactggct gcaaaacggc
     5041 gcggggcaga aaaccgtaga tatcctgatc cgcatcgcac tcattctttt cttctcggcg
     5101 gttggctgga cggtgctcgc cagtttgatc gaaaaccggc tggcttcgga tattcatggc
     5161 cgcccgctac ccagcgcccg tacgcgtacc ctgctgacgc tgttccgtaa cgcgctggcg
     5221 gtgattatca gtaccatcac catcatgatt gtgttgtcgg aaatcggcgt caatatcgcg
     5281 ccattgctgg caggtgccgg ggcattaggt ctggctatct cgtttggttc gcaaacgctg
     5341 gtaaaagata ttatcaccgg ggtatttatt cagtttgaaa acggcatgaa cactggagat
     5401 ttggtgacta tcgggccgtt gaccggcaca gtggaacgga tgtcgattcg ctccgtgggc
     5461 gtgcgccagg ataccggggc gtatcacatc attccgtggt cttcgataac cacctttgct
     5521 aacttcgtcc gcggtattgg ttcggtagtg gcaaactacg atgttgatcg ccatgaagat
     5581 gctgataaag ccaatcaggc actgaaagat gcggtaacgg aattgatgga aaacgaagaa
     5641 attcgcgggc tgattattgg tgaaccgaac tttgccggga ttgtcggctt aagcaatacc
     5701 gcgtttacac tgcatgtttc gttcaccacg ctgccactca aacagtggac ggtacgcttt
     5761 gccctcgaca gccaggtgaa aaaacatttc gacctggcgg gcgttcgcgc gccagtgcag
     5821 acttatcagg tgctgcctgc tccgggcgcg accccggctg aaccgctgcc gccgggggag
     5881 ccaacgcttt aacgttggcg attaacaaaa cggcggcgct gctcgtcgtt cataaaggtc
     5941 caggcaataa agcgactctg cttttgcccc tgggccatct ctttttttac caccttcacc
     6001 gcgcccacgt cggtcagagc acgatacaac ggcggtaagt tttcaccacg agataccagc
     6061 gaggtaaacc acatcacctg cttcgcgaag cctttgctct cttcaatcat ctttttgata
     6121 aagttgactt caccgccttc acaccacaac tcctgttgct gaccaccaaa gttcagtgca
     6181 tcgtctttgt tcagccccag gttacggcgt ttacgctcac tacctgcccg cgccgcagcg
     6241 gcggaatcgt ggaatggcgg gttacacaag gtcgcgtcgt attgctcgtt tttgtggatg
     6301 atgccgttaa aaatcgcccc actctctttt tgccgacgca gacgaatggc gcggttaagc
     6361 cccggattag cactgataat cgcctgcgca ctgcttaacg cctggctgct ggtttcacta
     6421 ccggtaaaac gccagccata ttcatgtacg ccaatcagcg gataaataca gttcgcacca
     6481 acgccgatat ccagaatgct ggcattcgct ggaattgttc cgcttgcttc agccagtaaa
     6541 tcggcaaggt gatgaatata atccgcccgg cccggtactg gtgggcagag aaaaccgtcg
     6601 gggatatccc agttcgctac ggcgtaaaaa tgggccagca acgccttatt gagcgccttc
     6661 accgccagcg gattggcaaa gtctacgctt tgctcccctg cgggtgtaag cgtgaggaat
     6721 tgcctgagtt caggattgac ctgacaaagc gtggcgagat cgtagcggct gtgatgacgg
     6781 ttgcgcggat gcaaccccgg tttctgggcg gacatagcat tctcctttaa gcagcggcgt
     6841 aagatacccg cataacgcga cagggtaaat atctggctgc gtttatcatt ccccaaaacc
     6901 ttaacatggc tatggtttat ggcaattcat cataaaaatc agcattatgc atgtcattga
     6961 tttgtcttat tttgtaaaaa tatatcgttc aaaaaataag catatctctg tcaatttttt
     7021 gcgtcatttg catcgcgcag gcgacctaca cttatcaggc actacccgtt gtatttatga
     7081 ggatgtcatt atgaaaaagt gcctcacact actgattgcc accgtcctga gcggaatctc
     7141 tctcacggct tatgccgcgc aaccgatgag taacctggac agcggtcagc tacggcccgc
     7201 cggtaccgtt tcggcaactg gcgcatcaaa cctaagcgat ctggaggata aactggcaga
     7261 aaaagcgcgc gaacaaggcg cgaaaggtta tgtcattaat tccgcaggcg gaaatgacca
     7321 gatgttaggt actgcaacca tctacaaata accccaccaa cgtaactgca ttcgcctctg
     7381 gatgcagttt tcgcctgttt tgtaatcaaa aaacatcaaa tatgaacgca atgtaaataa
     7441 atgtatttct ttttcgcgca atgggtgata gaaaatcgca tcaagtgata atgcttatca
     7501 aaattactat cacttgcacg aacactatca cgggattaac agtggcatcg catccgcaga
     7561 gatgctttct cgtggcagtg aaaatttcaa catataagaa aaagtcacct gcaaaatgga
     7621 aaacaatcgc aatttccctg ccagacaatt tcattcgctc acgttctttg ccggtctttg
     7681 tattggtatt acgcctgtgg cacaggcact cgccgccgaa gggcaagcta acgcggatga
     7741 cacgctggtt gtcgaagcat caacgccttc gctttatgcg ccacaacaat ctgccgatcc
     7801 gaaattctcg cgtccggtag cggatactac ccgcacgatg acggtgattt ctgaacaagt
     7861 gattaaagat cagggcgcaa ccaaccttac cgacgcgctc aaaaacgtcc ccggcg
//
LOCUS       DAEEGA010000141         7779 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEEGA010000141 DAEEGA010000000
VERSION     DAEEGA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.141"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            1..1329
                     /gene="yjeM"
                     /locus_tag="KLH46_003726"
                     /pseudo
     CDS             1..1329
                     /gene="yjeM"
                     /locus_tag="KLH46_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1480..4791)
                     /gene="mscM"
                     /locus_tag="KLH46_003727"
     CDS             complement(1480..4791)
                     /gene="mscM"
                     /locus_tag="KLH46_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBE0380255.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4813..5781)
                     /gene="psd"
                     /locus_tag="KLH46_003728"
     CDS             complement(4813..5781)
                     /gene="psd"
                     /locus_tag="KLH46_003728"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBE0380256.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5878..6930)
                     /gene="rsgA"
                     /locus_tag="KLH46_003729"
     CDS             complement(5878..6930)
                     /gene="rsgA"
                     /locus_tag="KLH46_003729"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBE0380257.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7025..7570
                     /gene="orn"
                     /locus_tag="KLH46_003730"
     CDS             7025..7570
                     /gene="orn"
                     /locus_tag="KLH46_003730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBE0380258.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1761 a         2021 c         2103 g         1894 t
ORIGIN      
        1 aatgatggct gaaatgggag ctgcttatcg caaagaagaa ggtggtatct attcctggat
       61 gaataatagt gtcggaccac gttttgcctt cattggtacg tttatgtggt tttcctctta
      121 tatcatctgg atggtgagta cctccgcgaa agtttgggta ccgttctcaa cattcctcta
      181 tggtagtgac atgacccagc actggcgtat tgccggactg gagcctacgc aggtggttgg
      241 tctgctggca gtagcatgga tgattctggt caccgtcgtt gcttcaaagg ggattaataa
      301 aattgcccgc attactgcgg tgggcggtat tgcagtaatg tgtctgaatt tagtattgct
      361 gttagtaagc attactattt tgttattaaa tggtgggcat ttcgcgcagg atattaattt
      421 ccttgcatca ccgaacccgg gttatcagtc cggtctggca atgctatcgt ttgtggtatt
      481 tgctattttt gcctatggcg gaattgaagc ggttggtggt ctggtcgata aaacggaaaa
      541 tccagaaaag aactttgcca aaggtattgt ttttgccgct attgttattt caatcggtta
      601 ttcgctggca atatttttat ggggcgtcag cacaaactgg cagcaggtat taagtaatgg
      661 ttccgttaac cttggcaata ttacctatgt gctgatgaag agcctcgggg tgacgctggg
      721 taacgcactg catttgtcac ctgaagcgtc attgtcgctg ggcgtatggt ttgcgcgtat
      781 taccggactt tcgatgttcc tcgcttatac cggtgcgttt gctattcacc gctgaaagcc
      841 atcatccagg ggacgccgaa agcgttgtgg ccggaaccga tgacgcgcct gaatgcgatg
      901 gggatgccgt ctatcgccat gtggatgcag tgcgggttgg ttactgtctt cattctgctg
      961 gtttcgtttg gtggcggtac cgcatcggcg ttctttaaca agctgacgct gatggcgaac
     1021 gtgtctatga cgcttcctta cctgttcctc gcgctggctt tcccgttttt taaagcacgt
     1081 caggatctcg acagaccatt tgtgattttc aaaacgcgta tgtcggcaat gattgcgacg
     1141 gtggttgtcg ttctggtggt gacatttgcg aacgtcttca ccattattca gcctgtggtt
     1201 gaagccggag actgggacag cacattgtgg atgattggcg gccctgtctt cttctcgctg
     1261 ttagcgatgg cgatttacca gaactattgc agtcgcatgg cgaataaacc tgagttagct
     1321 ctcgactgat catatgaaat cctgcaactc atcgtctctc cggcgatgag ttgtcaggaa
     1381 gtaatgcgta tggatgatac tctccagggc cggtgataac tgaagatgat gttaagctgc
     1441 taaatgagcc acgttgacat catttggcag cttcatgcat tacaaacttc ccgcctgacg
     1501 acctttgccc gcagacgtca gcgttctccc cgtttgttta ccgttgaggc tttccagacg
     1561 catctggaag ggcgggaatg gcatatcgat accgtgggcg tggaagcctg ccagaatcag
     1621 ctggtggatc tcatggcgta gcggcatacg gtgacccatc tcagcggcgt aaatacgcag
     1681 ctcgaaaatc tgaatcccct gttgcagatc caccaggaag acttccggtg ccgggttgtc
     1741 gatcaccagc gagcagcgac gcgctgcggt gagcaggatt tccgtcactt cttcactgtt
     1801 ggcatcggca ggggccggaa tcgtcaacac tacgcgcgtg accgagtcag agagcgacca
     1861 gttgataaac tgctcggtaa taaacgcctt gttcggcacg attatctctt tacggtccca
     1921 gtcgctgatg gtggtggcgc gggtgttaat ttttgtcacg ctaccagtaa gatcgcggat
     1981 cgttaccgta tcgccaatgc gaatcggttt ttcgaacagg atgatcaggc cagagataaa
     2041 gttggcgaaa atttcctgca aaccaaaacc gagaccaaca ccgagcgcgg caaccagcca
     2101 ctgcaatttc gaccactcaa taccaatcat tgagaagccg accagcccgc caatcagcat
     2161 cagcagatat tttgtgatgg tggtgatggc gtaacccgta cccggcgtta aatccaggtg
     2221 ctgcaaaatc gccagttcca gcagcgcggg caggttgcgc accagctgcg tggtgatgat
     2281 aaataccaga atggcaatca gcaccgcacc gagggtaatt ggctccagac tttctacgcc
     2341 ctgtaccgtg gaggtgacat cccacagcga aatattttcg aggaagccga aagcggaatg
     2401 gatttctgac cacagcacaa tgaccgaaag cagggcgatc aacatcaaaa ttgagcgcac
     2461 cagccgcaag gattgcgcac tgatggcatc gagatcgact tcgctttcat cgacttcaat
     2521 tgctccttcc gggctactgt gatgatgcgc ttcctcttcg ccacgcgcac gttgcgctaa
     2581 catctctgcc cggcgatgct tcgcccggtc aaacgccagc ctgcgacgct ggatcagcat
     2641 ccagcggcgg ataacgtgat aaaccaccag tagcaggaac cagatggcaa ccgaggtttc
     2701 aagcctcgct aacagtgcct gcgccgttgc cagataaccc accgccgacg ccagaatggc
     2761 aaccagtggt gcgccaatca tcatgttcca cagcatatgg ttggtaatgt tgtcgccgct
     2821 gccttctttg ttgagataca acggaatacc ggcttttttc aggcttaggg tgaccaccgc
     2881 cagcgcaccg caaatgagga taaagcaaag ccgtcccagc gaaccggaaa attcgcggtc
     2941 gtcgaggtta tcgaacatca tcagcgccat aatcagcccg atgctcatca ggtagtagcg
     3001 catcccacgg gaaacacgtt cgcgcggcca gccaaaatga gcgataaaca agccgttcgg
     3061 gcgggcaaag gtggcgcaaa tcatcactac ccacagcagc ggcacggtgg ccgttacacc
     3121 atcgccaatc gcgaccgcca gcggataagg ccacgcctcg cgcaagccgt aacccagcgt
     3181 catccacagc accggtaacg gtgacgcgac gagaatcgac cagaaaagag tgcgcaacgt
     3241 cagccagaag tgatcctgag tcactttgcc gactttcgcc gccgaacgtt caagaaaacg
     3301 ggtgaaatag cggcgcgagt aaatactgca accgaccaga atcaacgcgc caaacagcgg
     3361 caaaattgtc tctttgctgg tcagcatcat cacactggct ttgcccaact gactgaaggt
     3421 gtccagcgaa atgagacgac gcagatcctg ggcgatttcc agcggccagg cgatggtcat
     3481 cgggcgcacg tcagaggccc agaacagata gcggtgcgtt gcttcgttca cctctttcag
     3541 cgcatcctcc agttgcccgt tggagacttt cagcttggtc agttccaata gtagcgtgtc
     3601 gccaccctgc aacaatgagt tcagcaactc acgctgagtg cgcagttgtg cttccagaat
     3661 acggttttgc tcggcggtca gcggctgacc gtcggcctga tggatttgcc gtagcagcgg
     3721 ctgtttatta agtaaatcct cataacgtaa ccgttgcaca cgcaactgcg ccatttcggt
     3781 atcaagctgt tgtggttttg gcatttctgg tagccgtgcc acctgcgccc gcagcgcttc
     3841 gccgagcagg ttggacgatc ccagccattg cgactgttca cgcagcgtat tcaacgcctg
     3901 ccggacctgt aacgtctggc tggcagcctg acgctgttgc gaggcaacga gctccatccg
     3961 ctgcgcctgt tgattcagcg ctgccgatag ttcgcggtta attttgaatt gcgcgacgat
     4021 atctttcggc aaatcggcac tgttttctgc cagtaattcg gtactttcca gcgcccgctc
     4081 cgcctcaagc tgacgttggc tattcaactg attacgtaag gcctgcaaat acgcatccag
     4141 ttgctggctc tctttttccg ccagctctga gcgtaagcgc gctaattcct ggcggttatt
     4201 ggcagacagc tgcgccagct ccagttcatc aacgagcgcc ttaagacgtg cagagtcaga
     4261 ctgcaacgcg aaattttgtg cctgattgag cggagtattg ccggtaagcg ttcccaggcg
     4321 gcgctcgatc tcatttaact gacggcgggc gtcggtttgc tgttgcggca gttgattcag
     4381 cgaatcggca atctcgcggg ctcgctcctg ctcttgctgg gcctgacggc ttttatccag
     4441 caactggctg ctgacctgga gaatttcctg attcagcgcg tcggtagaca ttcccggcga
     4501 cacgctgcgc ggctcgtcac gaatgttgtt taattgtgcg cgcagagtag cggagagttt
     4561 cggataatta tcgataactt gctgatattg tttgatgcgc tcaagggaac cttttcgttc
     4621 ctcaagcgca tttaaggcag actggagcgc ctctacgact tccggctgtg cgggtttcgc
     4681 cgcttttgcc tgctccagtt cctgagtgat ttgtttgcta tcgggggccg tcgcggcgta
     4741 cgccccccaa ctgaggcacc aggccatcag aaaagtgata atcaggcgca cgtcagcgtt
     4801 tcctttgatg gattagacct ggtctttttt atcgtcaacc aatgggctgg cgtcgtgttc
     4861 tgcttcgatc tcttcagcag gaagtggggc gggttcagcg tctggcgtaa caaaggtttc
     4921 ggtagatact gccagcggct ggccaatttt cgtgacagac aggctttcca gttgctcaac
     4981 cagattcact ttacccggtg caaacaggtt gataacggtg gaaccgagtt taaagcgacc
     5041 catttcctgg cctttcagca gtgccacaga accgtcgttt tccccggcag gccaggtcca
     5101 gcgcttgatg ataccttcgc gcggcggcgt aatggtgccc gcccagaccg tctcaatgct
     5161 gccaacaatc gtcgctccga ccagaatctg cgccattggg ccaaattcgg tatcgaaaag
     5221 gcaaatcacg cgttcgttac gggcaaacag attcggcacg ttctgagcgg tgagatggtt
     5281 aacggaaaag agatcgcccg gcacgtagat catctcacgc agaataccgt tgcacggcat
     5341 gtgtacgcga tggtagtcac gcggggagag gtaagtggtc acaaacgtac cgttgcggaa
     5401 caggtccgcc atcagatagt tgcctgccag cagggcttcg aggctgtagt tgtggccttt
     5461 ggcttgcagg attttatctt cttcgatttt acccaactgg ctgataacgc catcggcagg
     5521 catgaccagt acattcggat cggtatcgat tgggcgtact tcgtcacgca gcggacggac
     5581 aaagaattcg ttaaaggtgc ggtagctggc ggtgtccggc ttttgcgcct ctttcatgtc
     5641 gaccttgtag tatttaacga acagatcgat aaccagtttt gtcagccatc ctgcccgctt
     5701 gcttgcgccc caacccgcca ggcgagtaag ccatagtttc ggcagaatgt actgtagcga
     5761 aagtttaaat gaatttaaca aggtagcctc caggccattg ttttgtcgtt cctgatccgg
     5821 ccaacatgcc ggatcctgaa aaaaagggga cgattctaac gacggttagc ttaattgtca
     5881 gtcatccgta tcagaaaagt ttttacgcgt ttttacctgc gccatgcttt ccagaatacg
     5941 gtgatagttt tcgaaacggg tttccgcgat tttcccttcc tcaaccgctt cccggatagc
     6001 gcagcccgga tcggtatcgt gtttgcaatc gcgatattta cacagaccta aatagtcatg
     6061 gaattcgaca aagccctgag tgatttgttc cggctccagg tgccagaggc cgaactcacg
     6121 cacaccaggg gaatcaatca catcaccgcc gtgcgggaag tgatacagcc gagcggcggt
     6181 cgtggtgtgc tgaccgagcc ccgagttgtc agagacatcg ttggtcagga tctctttttg
     6241 cagccccagc aacgcgttca gcaggctgga tttaccgacg ccagactgcc cggcaaaaat
     6301 gctgatgcgc ccggtcaacg cctcttccag cggtttcagc ccatcctgag tatggctgga
     6361 aaccatcaat acgcgataac cgatgttgcg gtagatatcc atctgctcgt tgacgaacgc
     6421 catgccttcg tcgtccagca ggtctatctt gttgagcacg ataatcggct caatctgcaa
     6481 ggtttcgcag gccaccaggt aacggtcgat aatattgagc gacagctccg gcaaaatggc
     6541 ggagacaatg acaatctggt cgatgttggc ggctatgggt ttcacgccgt cgtagaaatc
     6601 cggacgcgtt aacaccgagg tgcgctcatg caccgcttcc acgatccctt tgacatttac
     6661 gccttccgcc gccggtttac ccggacgcca gactacgcgg tcgccggtta ccagcgaacg
     6721 gatggtacgg cgaatattgc agcggtgaac gtcgccatcg gcggattcca catcagcgtg
     6781 cataccaaag cggctgatga cgataccttc atcaggctcg ccaaacagat tgtcgtcgta
     6841 gtcgggcttc tccttagacg ttttaagacg acgctggtga ttggcgttca cgcggcgctg
     6901 ctggcctttg gagagtttat ttttactcaa tcgtactgac tcctggtcgc ccctgatggg
     6961 caaaacatct atgatacacg caattgtgga tcaatatagt cactgtgaat gggtggaaaa
     7021 tagcatgagt gccaatgaaa acaacctgat ttggatcgat cttgagatga ccggtctgga
     7081 tcccgagcgc gatcgcatta ttgagattgc cacgctggtg accgatgcca atctgaatat
     7141 tctggcagaa gggccgacca ttgcagtaca tcagtccgat gaacagctgg cgctgatgga
     7201 tgactggaac gtgcgcaccc ataccgccag cgggctggta gagcgcgtga aagcgagcac
     7261 gatgggcgat cgggaagctg aactggcaac gctcgaattc ttaaaacagt gggtgcctgc
     7321 gggaaaatcg ccgatttgcg gtaacagcat cggacaggac cgtcgtttcc tgtttaaata
     7381 catgccggag ctggaagtct acttccacta ccgttatctc gatgtcagca ccctgaaaga
     7441 gctggcgcgc cgctggaagc cggaaattct ggatggtttt accaagcagg ggacgcatca
     7501 ggcgatggat gatatccgtg aatcggtggc ggagctggct tactaccgcg agcattttat
     7561 caagctgtaa aatttttcct ggtcacgtaa gcgccggaat ccacttgcgg cggcgtgatt
     7621 ttgacgctaa atcgaacgtc ttgctgataa tttcagcact tgagataaaa acgcaaaaaa
     7681 aacttttttg gggggttgca gagggaaaga tttctcgtat aatgcgcctc ccgtaacgac
     7741 gcagaaatgc gaaaattacg aaagcaaaat taagtagta
//
LOCUS       DAEEGA010000142         7746 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEEGA010000142 DAEEGA010000000
VERSION     DAEEGA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.142"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..1016
                     /gene="ydfJ"
                     /locus_tag="KLH46_003731"
     CDS             <1..1016
                     /gene="ydfJ"
                     /locus_tag="KLH46_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HBE0380259.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1105..2565
                     /locus_tag="KLH46_003732"
     CDS             1105..2565
                     /locus_tag="KLH46_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBE0380260.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDIVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNLIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2601..2804)
                     /gene="ydfZ"
                     /locus_tag="KLH46_003733"
     CDS             complement(2601..2804)
                     /gene="ydfZ"
                     /locus_tag="KLH46_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBE0380261.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2982..3668)
                     /gene="ydfH"
                     /locus_tag="KLH46_003734"
     CDS             complement(2982..3668)
                     /gene="ydfH"
                     /locus_tag="KLH46_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HBE0380262.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3757..4503)
                     /gene="ydfG"
                     /locus_tag="KLH46_003735"
     CDS             complement(3757..4503)
                     /gene="ydfG"
                     /locus_tag="KLH46_003735"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBE0380263.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4640..6685
                     /gene="dcp"
                     /locus_tag="KLH46_003736"
     CDS             4640..6685
                     /gene="dcp"
                     /locus_tag="KLH46_003736"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBE0380264.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQLRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6730..7248)
                     /locus_tag="KLH46_003737"
     CDS             complement(6730..7248)
                     /locus_tag="KLH46_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HBE0380265.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7524..>7746
                     /gene="ydeI"
                     /locus_tag="KLH46_003738"
     CDS             7524..>7746
                     /gene="ydeI"
                     /locus_tag="KLH46_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HBE0380266.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1943 a         1813 c         1953 g         2037 t
ORIGIN      
        1 ccatcttgct ggtgacgctg cgtattattc agggattggg tgcaggtgcg gaaatttccg
       61 gtgccggtac gatgctggcg gaatatgcgc caaaaggtaa gcgcggaatt atctcctcat
      121 ttgtggctat gggaactaac tgcggcacct taagcgcaac ggcaatctgg gcctttatgt
      181 tcttcattct cagtaaagag gaactgctgg cgtggggatg gcgtataccg ttcctagcga
      241 gcgttgtcgt gatggtcttt gctatctggt tgcgtatgaa tctgaaagaa agcccggtct
      301 ttgagaaggt taacgacagc aaccaaccga cagcaaaacc tgcacctgct ggtagcatgt
      361 tccagagcaa atccttctgg ctggcaacag ggctgcgttt tggtcaggcg ggtaactccg
      421 gtttaattca gactttcctt gcaggctatt tagtacagac gttattgttt aacaaagcaa
      481 ttccaacaga tgcattgatg atcagttcga ttctcggctt tatgaccatt ccgttccttg
      541 gttggttatc cgataaaatt ggtcgccgga tcccgtatat tattatgaat acctctgcga
      601 ttgtgctggc gtggccaatg ctttctatca tcgtagataa aagctatgcc ccgagcacca
      661 ttatggttgc actgattgtg attcataact gtgcggtgct gggattattt gctctggaaa
      721 acattaccat ggcagaaatg ttcggctgta aaaaccgctt cacccggatg gctatttcaa
      781 aagaaattgg tggtcttatc gcttccggtt ttggtcctat cctggcgggt attttctgca
      841 ccatgacgga atcctggtat ccgatcgcca ttatgatcat ggcatattca gtgattggtt
      901 taatctctgc gctgaaaatg ccagaggtga aagaccgtga tttaagtgcg ctggaagacg
      961 ctgcggaaga tcaaccgcgt gttgtaagag ctgcgcaacc ttccagaagt ctgtaaaccc
     1021 tcaatccctt ctcttaccgg agaggggatt tttattcata taacaaaaca tatagcttgc
     1081 catatttaaa tttaaggaat tgttatggga aataatttgt tatcagcaaa agcgacactc
     1141 cctgtttatg atcgtaataa cctggcccca agaattgttc atttaggctt tggtgcattt
     1201 caccgtgcgc atcagggcgt gtatgccgat attcttgcta cagaacattt cagtgactgg
     1261 ggatattatg aggtcaactt aatcggcggc gaacagcaaa ttgccgattt acaacagcaa
     1321 gataatcttt ataccgttgc ggaaatgtcg gccgatgcgt ggacggctcg cgtcgttggc
     1381 gtcgttaaaa aagccttgca cgtacagatg gatggcttag aaaccgtgtt ggctgcgatg
     1441 tgtgaaccgc aaatcgcgat tgtctctctg acaatcaccg aaaaagggta tttccactct
     1501 ccggcgaccg gacagttaat gctcgagcac ccgatggtcg ctgccgacgt gcaaaatccc
     1561 caccagccga aaacagcaac aggggtgatt gttgaggcgc tggctcgccg taaagcggca
     1621 ggacttcccg catttaccgt catgtcatgt gacaacatgc cagaaaacgg tcatgttatg
     1681 cgtgacattg tcacttccta cgcgcaagcc gttgatgtaa aactggcaca atggatcgaa
     1741 gataacgtga ctttcccatc aacaatggtg gaccgtattg tgcccgcagt gacagaggat
     1801 acgctggcga aaatcgaaca acttaccggt gtgcgcgatc ctgctggcgt tgcctgtgaa
     1861 cctttccgcc agtgggttat cgaagataac tttgtagccg gacgtccgga atgggaaaaa
     1921 gcgggagccg aactggttag tgatgtgctg ccttatgaag agatgaagtt gcgcatgctc
     1981 aacggcagtc attcattcct ggcatatctg ggttatcttg ctggatatca gcacattaat
     2041 gactgtatgg aagatgaaca ttatcgtcat gcggcgtatg gcatgatgtt gcaggaacaa
     2101 gcgccgacgc tgaaagtgca gggcgttgat ttgcaagatt acgctaaccg attaattgca
     2161 cgctatagca acccggcgtt acgtcatcga acctggcaga ttgcgatgga tggcagccag
     2221 aaattgccac agcggatgtt ggattctgtt cgctggcatc tggcgcatga cagcaagttc
     2281 gatctgctgg cgctgggcgt cgcgggttgg atgcgttatg tcggtggtgt tgatgaacag
     2341 ggaaatctga tagaaatcag tgacccactg ttacctgtta ttcagaaggc tgtacaaagt
     2401 agtgccgaag ggaaagcgcg cgtccagtca ttgctggcga ttaaggcaat ctttggtgat
     2461 gatttgccag acaatagctt gtttactgca aaagtgacgg aagcgtactt gtctttatta
     2521 gcgcatggcg cgaaagcgac cgtggcgaaa tattccgtga agtaaaaaaa gcaatgccgc
     2581 catcggcggc attcacacac ttagttcatg ccgagacgaa tcaggtccag aggtgccagt
     2641 ttctcttcac aaccttcaac aactacggtt tttccgcggc ggatttgctc agcggtcaga
     2701 ccttcagtat caatcgacag gatcttgcca gtgtgaccgg tgccgctaac cataacacgg
     2761 ctgccagtgg tgattgcgtt acggttacga tcgtaggtcg tcataggtgt tttctccttt
     2821 ctgatttaca gtcgaagccc acgtaacggg cgcaatataa atactcctgt cgagtgaaat
     2881 attttttgtt tttgatcaag ctcacatctt ttttgatgca tgcatgagcc gtaaggcggg
     2941 gggaaaagcc tctcatcccg gatgggatga gagtaaatga attactcttc gctaaaccag
     3001 tcgctgtttt cctggcggat ctggcgcacg gattcgctga tttcctgcaa atgttgcgtc
     3061 attgcacgtt ctaccgcatc gccatcacgt ttttgcaggg cagagaaaat atcaagatgc
     3121 tggcgtaaca gcatttctgg tggagaaaca tggtcgaaac tcatatagcg cacgcgatca
     3181 acggtcgctt tcaggttctc aatggtatcc cacgccagtt gacagtcggc aatctgcgtc
     3241 aggagttgat ggaagttgtc atcaagttca aaaaaatcat ccagttgctt gcgctcaatg
     3301 gcaatgcgtt gctggtgaag attttgttcc agttggtagc actggctttc ggtaatcatg
     3361 ctcgccgccc gacgcgccac cgcgcactca atggcctgac ggataaaact gccgttgcgc
     3421 acctgggcca tggaaatttt gttgacgtag ctgccacgct gcggacgaat ttgaatcagg
     3481 ccgttttccg ccagtttaat aaaggcttca cgaaccggct ggcgtgacac attgaaacga
     3541 acagaaactt ctttttccga caacggtgtg cctggtgcaa tcaggcaatg gacaatgtcg
     3601 cgacgaagaa tacgataaat ctgctgattg acaggctgtg tgggattaag ttgcgtttcg
     3661 acggtcattc gttcttgctt gtgagtgagt taactgcatg agtctaccac ttttttgtga
     3721 caagcaataa cccggcagtt acgccgggta taaaaattac tgacggtgga cattcagtcc
     3781 ggcatagctt tgggtaaccg gcatcatttc cagggtattg atattgacgt gagcaggcag
     3841 cgttgacacc caccagacgg cttcgctgac atcttctggc gtcaatgcaa cggtattttg
     3901 ataggttttt tccgctttac cgtcatcgcc tttaaagcgg acattggaaa actcggtgcc
     3961 acccaccaga cccggttcga tgtcggtgac gcgcaccgcc gtaccatgca gatccgtacg
     4021 cagattcagg ctaaactgac gaacaaacgc tttcgtcgca ccgtaaacgt taccaccggc
     4081 atacggccag ctgcctgccg ttgagccaat gttaataata tgaccatgat tacgttcaac
     4141 catacccggt aagacggcgc gcgtcatata tactaggcct ttgttgttgg tatcaatcat
     4201 cgtttcccag tcttcaacgc tggctttatg cgcaggctcc atgcccaacg ccaggccggc
     4261 attatttacc aggatatcaa tattgctcca ctcggcaggg agcgatgcca gcatctcttc
     4321 aatagcggcg cggttgcgaa cgtccagttg ggcgatatac agattatctc ccagttcgtc
     4381 tgttaactcc tgcaaccgct cctggcgacg gccagtggcg ataactttat gcccttgttg
     4441 aataaaacga cgagtaatgc attcaccaaa acctgccgtt gctccagtta ctaaaacgat
     4501 catctcactg ttcctcaacg cttttgtgtg tcaataacat agcacgtgat gcttaagagc
     4561 gggtaatact ggtttgcaag attgcactcc gttatcagta agcctacgct atggaacacc
     4621 tcgcgttcag gagaagaaaa tgacaacaat gaatcctttc cttgtgcaaa gcacactgcc
     4681 gtatctggct cctcattttg atcaaattgc caatcatcac tatcgcccgg cattcgatga
     4741 gggaatgcaa caaaagcggg cagaaattgc tgccatcgcg cttaacccgc aaacgcctga
     4801 tttcaacaat actattctgg cactggaaca aagcggagaa ttaattaccc gcgttaccag
     4861 cgtctttttt gcgatgactg cggcgcatac caatgatgaa ttacagcgtc ttgatgaaca
     4921 gttttccgct gaactggcgg aactggctaa tgatatctat ctgaacggtg aattattcgc
     4981 gcgggtagat gctgtctggc agctccgtga atccctgggg cttgatagtg aatccatccg
     5041 cctggtggag gttattcatc aacgttttgt ccttgccgga gccaaacttg cacaagctga
     5101 taaagcaaaa ttaaaagtac tgaatacaga agctgcgacc ctgaccagcc agtttaacca
     5161 gcggttactt gcagcaaata aatccggcgg tctggttgtg aacgatatcg cgcagctggc
     5221 aggaatgagt gagcaagaga ttgcgctggc ggcagaggcg gctcgcgaga aaggtctgga
     5281 taacaaatgg ctgattccgc tgctgaatac cacccaacaa ccggcgcttg ccgaaatgcg
     5341 cgatcgtgcg acgcgtgaaa aactgtttat tgcgggctgg acgcgagcgg aaaaaaatga
     5401 tgccaatgat acccgcgcta tcattcaacg tctggtagag attcgcgcac agcaggcgaa
     5461 actgcttggt tttcctcatt atgccgcatg gaaaatcgcc gatcagatgg caaaaacgcc
     5521 agaagcagca ctcaacttta tgcgggaaat tgttccagcg gcgcgtcaac gtgcgagcga
     5581 tgaattagcc tccatacagg cggttatcga taagcagcag ggcgggttta gcgcgcagcc
     5641 gtgggactgg gcattttatg cggaacaggt acggcgggag aaatttgatc ttgatgaggc
     5701 gcagctcaag ccatattttg aattaaacac ggtgttaaat gaaggtgtat tctggaccgc
     5761 gaatcagctc ttcggtatta agtttgtcga acgttttgat attccggtct accatcctga
     5821 cgttcgtgtg tgggaaattt ttgatcataa tggcgtggga ctggcgttat tttacggtga
     5881 tttcttcgcc cgtgattcaa aaagcggcgg tgcatggatg ggcaattttg ttgagcaatc
     5941 aacgcttaat gaaacgcatc cggtaattta taacgtctgc aattatcaga aacccgctgc
     6001 cggtgagcct gcgttgttac tctgggatga tgtaataacc ttattccatg aatttggtca
     6061 tacgctgcac ggcctttttg cccgccagcg ttatgccacg ctttccggca ccaacacgcc
     6121 gcgtgatttt gtcgaatttc cgtcgcaaat caacgaacac tgggcaacgc atccgcaggt
     6181 attcgctcgc tacgcccggc attatcagag cggggcagca atgcctgacg aactgcaaca
     6241 gaaaatgcgt aatgccagcc tgttcaacaa agggtatgag atgagcgaac tgcttagcgc
     6301 cgcacttctc gatatgcgct ggcattgcct ggaagaaaac gaagcaatgc aggatgtcga
     6361 tgattttgaa ttgcgggcgc tggtggcgga aaatatggat cttcctgcta taccgccacg
     6421 ctatcgcagc agttatttcg cccatatttt tggtggcgga tatgctgcag gttattacgc
     6481 ttatctgtgg acgcaaatgt tggccgatga tggttaccag tggtttgttg agcagggcgg
     6541 attaacgcgt gaaaatgggc agcgttttcg cgaggcgatc ctttccagag gtaacagcga
     6601 agatctggaa cgcctgcatc gacaatggcg tggtaaggct cctcagatta tgccgatgct
     6661 gcaacatcgt ggcttgaaca tataaacacc accaatggct ggcctggaga tatcgccagg
     6721 ccagcctggt tatagcagta attgcagcag ctgggcgagg gcaaacctca ccgctaaagc
     6781 taataccgtt tcgcagtcgc cagcaaaatg cataaccgca gtgtagttct ggcctttaat
     6841 atgccacgcg aaccagacgg tacccgctgg cgtaccatct tcaccgccct ccggtccgcc
     6901 gtagccggta atggcaatac tgacatcagc atccgcacgc tctatggcac ctgttgccat
     6961 ttctgctgcc actttctcac tcaccgcaga atatcgttca agagactgct ggcttacgct
     7021 gaggattttc atctttgcct gatcggtgaa agtaacaaag cctgcaccgt aaaatttggg
     7081 tgtatcttca gctgcacaca gggcgctagc caacttaccg ccggtgcatg attcggcggt
     7141 agttaagcgt aattgatccg cgataagtct ttgactcaac gcggatgtca ggttttcaat
     7201 agtgtcggta tctgcgagtt gtacaatttt atctttatta atgttcactg ttttttgtcc
     7261 acgctattat atgtaaaggt gatttgcttt ttctgtatgc tcgtccaata taataatttt
     7321 caaacatata aatccgatta ttctgaaaac atgtttaaga ctcttcaatc ttcttaactt
     7381 tagtgagtca aattctctga atatttgaca gctagtgggc tgattttaaa tgccttataa
     7441 caattggcag tattcttatt tggcaattat aaaaatagca accagactta ataagtcaaa
     7501 ccaaacgcaa ttaaggagtt agtatgaaat ttcagtccat tgtattagca agttttcttg
     7561 tcatgcctta tgcgttggca gatgaccagg gcggtttaaa acaggatgca gcgccaccgc
     7621 cgcctcatgc aatagaagat ggctatcgcg gaaccgatga tgcaaaaaaa atgaccgttg
     7681 atttcgcaaa aaacatgcac gatggcgctt cggtttcact acgcggtaat ttgatttctc
     7741 acaaag
//
LOCUS       DAEEGA010000143         7738 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEEGA010000143 DAEEGA010000000
VERSION     DAEEGA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7738
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.143"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(1..361)
                     /locus_tag="KLH46_003739"
                     /pseudo
     CDS             complement(1..361)
                     /locus_tag="KLH46_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(700..1254)
                     /locus_tag="KLH46_003740"
                     /pseudo
     CDS             complement(700..1254)
                     /locus_tag="KLH46_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1397..1519
                     /locus_tag="KLH46_003741"
                     /pseudo
     CDS             1397..1519
                     /locus_tag="KLH46_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1781..2347)
                     /gene="ipgB2"
                     /locus_tag="KLH46_003742"
     CDS             complement(1781..2347)
                     /gene="ipgB2"
                     /locus_tag="KLH46_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HBE0380267.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2629..2943)
                     /locus_tag="KLH46_003743"
     CDS             complement(2629..2943)
                     /locus_tag="KLH46_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380268.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3021..3131)
                     /locus_tag="KLH46_003744"
                     /pseudo
     CDS             complement(3021..3131)
                     /locus_tag="KLH46_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3455..4132)
                     /gene="ospD1"
                     /locus_tag="KLH46_003745"
     CDS             complement(3455..4132)
                     /gene="ospD1"
                     /locus_tag="KLH46_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HBE0380269.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4503..4640)
                     /locus_tag="KLH46_003746"
                     /pseudo
     CDS             complement(4503..4640)
                     /locus_tag="KLH46_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4722..5020)
                     /locus_tag="KLH46_003747"
                     /pseudo
     CDS             complement(4722..5020)
                     /locus_tag="KLH46_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5609..5768)
                     /locus_tag="KLH46_003748"
                     /pseudo
     CDS             complement(5609..5768)
                     /locus_tag="KLH46_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5772..7001)
                     /locus_tag="KLH46_003749"
                     /pseudo
     CDS             complement(5772..7001)
                     /locus_tag="KLH46_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7021..7368)
                     /gene="tnpB"
                     /locus_tag="KLH46_003750"
     CDS             complement(7021..7368)
                     /gene="tnpB"
                     /locus_tag="KLH46_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBE0380270.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7368..>7738)
                     /locus_tag="KLH46_003751"
     CDS             complement(7368..>7738)
                     /locus_tag="KLH46_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0380271.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQILIREIKGSSH"
BASE COUNT         1988 a         1804 c         1689 g         2257 t
ORIGIN      
        1 tgttgttgcg ccttgctgcc aacgaagctc cactgcccat caagttcaca gataagggca
       61 acatcagcgt gagcgactgg cgcagatgtt atccgccggg gcgggagctt tttaatgtcc
      121 gtatgacggt attgatgccg attttcagcg tacggccagt atcgcgaact ccggcaccgt
      181 tgaaagccat ttcagtgatt tggtttttaa ctcccggttt acgggcctcg taacagtaag
      241 tgagctgaaa cacccggtga cagtcacggc aacggaatcg atcatgtcct tttgggttct
      301 gcccgtgacg gtaaacctga acggactggc aacgggggca atgaaagtta actgtgacca
      361 tgaaagaacc taaaagcggg tattataacc cctaattcaa ctaattggag gcattgtcat
      421 ttttaatcta tggatacact taaaaatcct ctcaccttac gttaaaaacc ttctcatgtt
      481 tagagttttt tattatgttt atcataatga tatgacgatc tgagttttta aaatcaagaa
      541 gaaaagataa gttttttaaa tgcacagtgc ctgtggataa ctttgcgtaa atccggagga
      601 cagatcactc tgaagcaaga caacagcatc tgaaaacaat atgctcacgt ttcaaccaca
      661 gggcgtaagt gatcctaccc acgtaatatg gacacaggcc taagcgaggt tctggttttc
      721 aaattgttcc ggactgaggc cgccacacca actgtgccgt cgccagcgat tgtaatcaca
      781 ttcgatataa ttaaacactg ttgcccgcat tatttcccgg ctgataaagt gttctccgtg
      841 gagacattcc actttcagcg aatgaaagaa gctttccacg caggcattat cgtagcagca
      901 gccctttgcg ctcatactgc cacgcagatt atgccacttc agcagcgcct gataatctgc
      961 tgaacagtac tgacttcctc ggtccgagtg aacgataacg ttccggggtc tcttacgccg
     1021 ccacagcgcc atctgcaggg catcgcaggc cagttgcgcc gtcaggcgtg gtgacatgga
     1081 ccagccaata acggcacgtg accacaggtc aatgaccaca gccagataca gccagccttc
     1141 atctgtacgt aagtacgtga tgtctcccgc ccacttctgg ttcggaccac tggcgtaaaa
     1201 atcctgctcc agcagatttt ctgacacagg caggccatgt gcgcggtaac tgaccgtatt
     1261 gctgggtaag atcaatttgc tcactggtat ttactacgtg catcgaatga gtgatcaaga
     1321 gccgtatgac gcgagagtgt cacgtacggt tccgtaagca gccggagagt gaaactctct
     1381 ccggctgact cgactcgcag gttccgacag cggtgacgaa catgcagcgg tgttgttctc
     1441 gctgatcggc acatgccgtc tgaacaatgt ggagccagaa aaatggctgc gttacgttat
     1501 tgaacatatc caggactgac cggcaaaccg ggtacgcgat ctgttgcctt ggaaagttga
     1561 tctgacttct cagtaaatat caatatgatt ctgacgagcc gcttaccttt gagcagagaa
     1621 aggggagagg gagcccacat ccatgagcta ggaaaataca aaaactggct atacaacaat
     1681 tcaatatatt aggacttact tgcgaggaga aatatagcac cgctcaggca attcctgggt
     1741 atttactttt cttagtttct ttactcggga tgttctgtca tcagaaaggc gattctaaat
     1801 ttgtaatata gtcactcata ttcccacgta atttctttag atcaatatat tgcacatttt
     1861 tctcaaaaaa ctgatcggaa ataagatttg caacattgct tgttgtatca tttttaacgc
     1921 ttctattcat cccttcacaa agttcgtcca ttttcttttt gaaataactg cttcctgata
     1981 caagatgatt tatggaactt tgtgcagcat tgatgtctag agaaatgcga taatctttct
     2041 caacctgttt aaatatctgt ttacggagat tatcgccgat ttttcggctg tgttctgcac
     2101 aaaacttatc aatacattgg tttaccaccc gggatacgta gacttttttt tcatcatttt
     2161 tccaatctgt aatattttca ccgatttgtt ctgaaacaga gctcaaaata ctagtattta
     2221 ttttggttga tgatattttt actatccttt tccccattgt acagcggata atctcatcca
     2281 cctttcctga aaaatatctt ttgtgagata aactgattcc aaaattatta aaagatgttc
     2341 caagcattat aatcaccttg tcatgtaaaa tagtaacatt gtcattattt tagctgattc
     2401 aaacaattct gtaaatgctt tgagttcttt actcccttgg ctcaatgatg gaccgtctgg
     2461 aatcccagag actaaactct ctgagaaaga gagtatgaaa aaacttgggg cagtccctaa
     2521 atgaagggac caacagcggt aaaataaccg gcatcattca taatgatgcc ctgttagttg
     2581 tagttatgaa gatttacgtc attaaaattg ctgttcgtag tgtcagtcct atggtctggc
     2641 atcagctgag gatcgtagcc gatacatcac tggcttcacg acattcgtgt tgaggctatt
     2701 catgaaaact ccacgctgaa aacgccgttt tgtataaacg gtcacggtat gcccggagcc
     2761 actgttgctg atgaattcga taaaaccttg gcgtttcttg aagctatcgt taatgcggat
     2821 aatgaaacaa cgattggtga gattcgctct tttgccgacg ccctggacgc tgtccggttc
     2881 aatcgcaaca aaatcaaccg gcagctgagc aaacccaacc ttgcctctct ggcgctggag
     2941 catgaagtga tttggctggg acgtagccgc tgacagcttc tataaaagta tgatcctacc
     3001 cacgtaatat gaacacaggc ttaagtgagg ttctggtttt caaattgttc aggactgaga
     3061 cctccacacc aactgtgccg tcgtcagcga ttgtaatcgc acttgatata attaaacact
     3121 gtatatttca tttgtggagt ctccagtctg agagggcttt atgcattccg tattgggcac
     3181 tatgatgcca gaaatctgca aggctccaca ccttcctgta tttcccttcc tctatcacag
     3241 tgcactttac ttaatagggt gggatgtgtt cattacattt attgtgtttt ttatgattgt
     3301 ttttgcgtcc agtgtcgttc gtcatgggaa actggttcta tgggtagtat cgctcagtct
     3361 agttaactgt ttgtttcttt ttagaaatta agcagatcac acaattcggg gagagatcct
     3421 tctaagtgca attctttgca gctatttagc acacttaaaa caactcttgt attttgtcag
     3481 aaataatatt aaacatgtct tgttcattat tgttaagtct gtctgctatt tctgtaagtt
     3541 ttatcccact atgatggcat atatttatat atgccatcat agcctcgata ttccctttcc
     3601 ctgcggccag aaataatcct ggtaacccct cattatctct tacatccaat agtttaatcg
     3661 tttcttttgg tgataaatca aaagttttaa taaaatctcc ataagcttga atcgcagcgg
     3721 aatgaccttc ttgaagagct atgaacaaag cagattcccc agcattattt cgtgctgcaa
     3781 gaacttctag tttttcctct ttgggtattt cggctgccaa cagcgattca ccgtatttac
     3841 gcgctgcatc ggcatttcca tgccaaatca atgaaaacaa attatggcgg ttatactcct
     3901 tattattctt tgtcggaatg gatatgttct gtattttggc caattttctg aaagaaacac
     3961 cactatatcc cccccctgat ttttcttcat tgatggcgtg ggatatagcg gtatttgtca
     4021 cgttaatgat attatttttc attcctttat tttcattagc agtattgctg cctattatta
     4081 ggtgcatatt tttgttgttt gctggatgta atccatagtt atttattgac atttttgttc
     4141 atcctttttg ctaaaaaaaa caataacctt tttttgtaat gtaatagata ttagtctatt
     4201 gctaatctaa aaaaaccatt gccacattga atggctatat tcaccaataa attctttcga
     4261 tttcgtatat tgacatatcg tagatatatc ttatccctaa gattcaataa tcgagtccgt
     4321 ttaccattct gtgcaagcgc cttcactcag aagtgaccgt caaggcgatc accaaacttg
     4381 ataataaagc agctcattgc catacgccag tccttaaggg gcgttgtcca tttctgtgat
     4441 gctgcctaga tttaactgcc agctccggct tatctacatc taaatatgtg agcctaatac
     4501 cgctacactt tgtccagact atgtttttct ccgggaagtg ggctaaccgt ctccataaag
     4561 tgacgaactt ttttcaacag tggccatatt gaacggcact gatgattacg ggttattgtc
     4621 tcatgaagag ccggccacat cactgacagc agtgacttca ggccttcagc accagacagc
     4681 ctgaaccagt taatccatcg cccgacaaat ttagtaatct agggctggtt tttcttcagc
     4741 tgttgcggtt cgaaagtgcc attacgatca cgcagagtac ggatttccag aggttcatct
     4801 ccagtagtga taatcttcgt taagcaacca ttgcgggaat tgctgccaga tctaagctga
     4861 tttttctcgc acccaagatg atgagtcata ttagcattga gtacggtttc attttcagca
     4921 tccaatcaaa ctgactgaga tcttcaggat ttttgagact tttggctagt tcattagcta
     4981 aagcccgcaa cttattagat gtcaactgtt tttcattcat aaattcacct tcatttgata
     5041 tcaaattaaa tagttcaaca tcagagaatt atacaaactt atgtacaggc tcctatgatc
     5101 ttcgaatcct gaagattata gtgaaaataa gttttcacgt tgaaatccca catagttagt
     5161 atatgataaa taatcatgga tgacctgatt aacctcttgg ggtagtgctt ttatagaaga
     5221 ataaatattc tactaattta atacatcacc taaaatagta ttgtttttta tggttggttg
     5281 aaggtcgtgg tgatgatgtt agtccttttg agaatattcc agtgccagat cttacttata
     5341 ataaaggggc ttggcagttt ggataaacta ttgttcttat tccactaata caaacttttt
     5401 gtaaagtata ttaaatatac ttcatttcat actgaaatcc ccggaagtat tcaatcaaac
     5461 aattacacca taaaaggaag agggggccct aattaagttg tcaagcatgt ttatgatacc
     5521 tgcagggtat aaattttctg gctgcaagca gacacatata aaacgtcttt attgatccta
     5581 cccacataat atggacacag tcgtaagtga ggctctggtt ttcaaattgt tccggactga
     5641 gaccaccaca ccaacggtgc cgtcgccagc gattgtaatc gcactcgata taactaaaca
     5701 ctgttgcccg cactatttcc cgatggataa tagtgttctc cgtggagaca ttccattttc
     5761 agcgaatgaa ttttcatctg ttgctgtatc cagtcataca gtgactgcat cagtggcgcg
     5821 gctctggctt ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg
     5881 atggcataca gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt tggcactctt
     5941 gcatgcacat cgtggatttt tctccggaca tgagccatac atgcggcttc cgctattctg
     6001 ccggattcgt acaacgcccg gtaaccaccg taagcatcgg cctgaagcac tccgctgtaa
     6061 ccggccagat gattctgtgg atggatacct ttccggtccg gtgagtacgc gaaccagacc
     6121 gccggaggca tttccgaacc ggcgttacgg tcatcacgaa cgtagaccca cagccgggca
     6181 gtccttgttt taccgctgcc cggctcctgg accggaacgg ggatatcatc ggcatggact
     6241 ttaccgggca tcagcacata ctggcgcagg atgtcataca gcggttccag cagttcagca
     6301 acagcacctg tccagcgccc cagtgtggca cggctcagct ccacgccctg acgacggtat
     6361 atttctgact ggcggtataa cggcagatag tctgcatatt ttccagcaac aacatgggcc
     6421 agaagccccg ctccggcata actgcgtgca atgggttttg aaggcaccgg tgcctgcacg
     6481 atatggtcac accggcaaca ggccagtttc ggacgttgtg tttcgataac cttaaaggcg
     6541 ctgctgataa gctccagctg ctctgacaca tcacatccca gagaactgag ttcgccacca
     6601 caggccgggc agcattcctc ttccggacgg atgacccggg tttcacgggg aagtgaggca
     6661 ggtaacggtt tacgggctga agactggcgc aggggagatg gcagtaccgg gtcatattgc
     6721 tcacccagcg tttccgccat ttcttcctga agtgcgctga ttcgctcctg tgcttccagt
     6781 atctgccgtt cggtttttgc gcgaagtttt tctgagcttt taccgaactg catgcgctgc
     6841 agtttcacaa ccagcgcctt cagccggttg atttcggaag cataagccgc cacccgctgt
     6901 gagagcaggc ggttgtattt cgccatctgg cggatggtgt cctgttgcgt ctgcaacagt
     6961 gcccgcaggc gggcattctc atgagcaagt gaggtgtcca tatcctcact ttacaacggg
     7021 ttatatgcgg atgccagcgc gttccgttcg tttcgggtgt ttccagtcga tgccctcaag
     7081 aagcatggat aactgggccg gagtaaggtg caccttgccg tcacgggtga ccggccagac
     7141 gaagcggccc cgctccaggc gtctggtaaa gaggcacagt ccgtcactgt cagcccacaa
     7201 cacttttatc tggtcacccc ggcgtccgcg gaagatgaac agatgcccgg agaacgggtc
     7261 atccttcagg acgttctgaa cttttgaggc caggccgtta aagccatttc gcatatcggt
     7321 gatacctgca accagccaga tacgcgaacc tgcagggaaa gatatcatca gtggctgctc
     7381 ccttttattt cgcggataag tatctgtaat aacgccggcg tcagtttacc tttaagcctg
     7441 agagttccgg ccggcagaac cagctcacag cacagactgt cggacggtgt atttatctgc
     7501 tctggttcct gtgccggagc cgggatttta ttatccggct ccggcgttaa cgtcacggga
     7561 agcagtgcag gcatattttt tccggaaggc agcaggccac ctttccggta ttgatggcgc
     7621 cagttgaaga gcaggttatc gttgattccg ttttcacggg cgatctgcgc cacacaggct
     7681 ccgggctgca gtgactgctc cactaaagcg attttaaatt cataaggaaa attaggcc
//
LOCUS       DAEEGA010000144         7721 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEEGA010000144 DAEEGA010000000
VERSION     DAEEGA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.144"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..830)
                     /locus_tag="KLH46_003752"
     CDS             complement(<1..830)
                     /locus_tag="KLH46_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HBE0380272.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     "
     gene            1012..2436
                     /gene="sbcB"
                     /locus_tag="KLH46_003753"
     CDS             1012..2436
                     /gene="sbcB"
                     /locus_tag="KLH46_003753"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBE0380273.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2479..2706)
                     /locus_tag="KLH46_003754"
     CDS             complement(2479..2706)
                     /locus_tag="KLH46_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBE0380274.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="KLH46_003755"
     CDS             complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="KLH46_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBE0380275.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="KLH46_003756"
     CDS             complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="KLH46_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBE0380276.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="KLH46_003757"
     CDS             complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="KLH46_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBE0380277.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5528..6511)
                     /locus_tag="KLH46_003758"
     CDS             complement(5528..6511)
                     /locus_tag="KLH46_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0380278.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6557..7381)
                     /locus_tag="KLH46_003759"
     CDS             complement(6557..7381)
                     /locus_tag="KLH46_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE0380279.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="KLH46_003760"
     CDS             complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="KLH46_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBE0380280.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2068 a         2013 c         1811 g         1829 t
ORIGIN      
        1 tcgaagattg gacggcggat aacattaccc atcttgctat gctctttcgg tacggtccat
       61 acgttgtcca gcaggtcaaa ttctgtcttt gttgccagcc taagctctga gagcctcgcc
      121 ccccacagca taagcatctg atgaaggagc ttatttgacg tagacgcacg gcttctttca
      181 atagcaagcc aaatcttagc cagttcgtga tacgacagta cccgatcccc tacctcagcg
      241 cgggagccga agtcccttgg ctggatgctc ataattgcgc aactatctat caactgacgc
      301 cgcatgcacc aactaattgc tgatcttagt tgacttagca cctgccttgc tcggcgtgga
      361 ttatcttttt cttcttcggt aagcaggtca acccattgct taaccgtgat agaagatgcc
      421 ggacgattag ggaaggcgtc atgcatgcgc ttcataaccg ttgatcgata aagtgcctgg
      481 gtcttttctc tgagagttgt agagacgtag ttgtcgaacc agtagtcgag acactgggcg
      541 accgtcatgg agttctccac cttctcttca aaataggtgc gtggatccgt tcctgagaaa
      601 tagagctttc gcaagtcagc agtgatctgt ctggcatcct tcaaagacag ggatgggtat
      661 cgaccaagcc caagtcgatt aggcttgcca tgccaacgat agcggtactg gaactggatg
      721 acccccttcg gtgaaattcg tacgctgagg ccatcggcat cagccacttc ttgtgggccc
      781 gaatatggtt taccataaat agtacgcagt tttgtgtcgc ttatagccat aaataatatt
      841 tggtacgctc agaaataata gtttggcaca catctggtac acaatatcac atggacaaca
      901 gcgcacagta aacaactata tttgacgttc gtagacatac tcaggtgaaa aaagggttgc
      961 ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac
     1021 acagataaac aacctacctt cctctttcac gattacgaaa cctttggcac gcaccccgcg
     1081 ttagatcgcc ctgcacagtt cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc
     1141 gaacccgaag tcttttactg caagcccgcg gatgactatt taccccggcc tggagcagta
     1201 ttaattaccg gtattacccc gcaggaggca cgggcgaaag gagaaaacga agccgcgttt
     1261 gccgcccgta ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat
     1321 gtgcgtttcg acgacgaagt cacacgcaac gttttttatc gtaatttcta cgatccttac
     1381 gcctggagct ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt
     1441 tatgccctgc gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt
     1501 cgccttgagc atttaaccaa agcgaatggt attgaacata gcaacgccca cgatgcgatg
     1561 gctgatgtgt acgccactat tgcgatggcg aaactggtaa aaacgcgtca gccacgcctg
     1621 tttgattatc tctttaccca tcgtaataaa cacaaactga tggcgttgat tgatgttccg
     1681 cagatgaaac ccctggctca cgtttccgga atgtttgggg catggcgtgg caataccagc
     1741 tgggtggcac cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggcggatttg
     1801 gcaggagaca tttcgccatt actggagctg gatatcgaca cattgcgcga gcgtttatat
     1861 accgcaaaag ccgatcttgg cgataacgcc gccgttccgg ttaagctggt gcatatcaat
     1921 aaatgtccgg tactggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga
     1981 attaatcgtc agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt
     2041 gaaaaagtgg tagcgatatt cgcggaagcc gaaccgttta caccttcaga taatgtggat
     2101 gcacagcttt ataatggctt tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg
     2161 gaaaccgagc cgcgtaattt accggcactg gatatcactt ttgttgataa acgaattgaa
     2221 aagttgttgt tcaattatcg ggcacgcaac ttcccgggga cgctggatta tgccgagcag
     2281 caacgctggc tggagcaccg ccaccaggtc ttcacgccag agtttttgca gggttatgct
     2341 gatgaattgc agatgctggt acaacaatat gccgatgaca aagagaaagt ggcgctgtta
     2401 aaagcacttt ggcagtacgc ggaagagatc gtctaatttg aagccgccgc cgctggtacg
     2461 gcggaggaca atgttgagtc aggctttttg aacggtgatg ctccacgctg catcgccaat
     2521 ctgctgataa tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt
     2581 tgcctgggtg caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc
     2641 tgcttttgct tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat
     2701 cgccatcttt acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg
     2761 gtcgaacata cacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa
     2821 tccagccctg ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac
     2881 ctgcaatact ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt
     2941 gtgcatcagc tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgcctcca
     3001 cgaagatccc taacaccagg aaaacgcccc agttaatgta tttcacgtca cccgcgacca
     3061 gaaattgaag aatattggcc gttggggaag tgactcctaa cccaaacatg cgtccggttg
     3121 cttcactcag gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg
     3181 gatgccagcg tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg
     3241 cgactttgag ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca
     3301 aaacggtaac caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag
     3361 tagtgtagtg ctgcaaggtt tgatttaaac cactggcgtg tggagaacgc atcaccgcac
     3421 tcatcaccat ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat
     3481 accaggtccc ggtggcacat ccacccgcca gaacaattcc cagcccgaaa agatacccac
     3541 ctataacagt accaagccac gggaacgccc cggcttcgta agtcagtagg cccgcctgaa
     3601 ttaacgcaaa aacccccacg ctttgtgccg aaattgcaat cagcaaggcg taaaacatgc
     3661 gattattttt cgcgatatac atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct
     3721 gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa
     3781 attaattaac cagatgaatg ttaatgggga aatttttcat gactggtgga atgcagacca
     3841 ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa
     3901 taaaaaaacc ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat
     3961 gctacgtctt cgtactgcgg aaccggatta cggaagctct tggtgacgca agccaggtaa
     4021 atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc
     4081 cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct
     4141 ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaaca
     4201 aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac caggtcgaag
     4261 ttgattgcca gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat
     4321 ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac
     4381 atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag
     4441 aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt
     4501 gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt
     4561 gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg
     4621 cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag
     4681 aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac
     4741 ggacgcgtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg
     4801 cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt gacgttcgct
     4861 acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac
     4921 atccacgatg gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg
     4981 atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc
     5041 gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc
     5101 agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc
     5161 ggcacatgac catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc
     5221 tgcatatagg ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc
     5281 acgcgagagg tgtttggagt aacgttatgc gacatggtta ttctccttta cggtgataac
     5341 cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga
     5401 tttttagtaa atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga
     5461 agcaaaaaat aaccgacgct aattaaacgt cggtttttaa tcattttttg acagcggcgc
     5521 attgtgccta aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc
     5581 tttgaatggc tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg
     5641 ctaaaatttc atctcgccac cagccgctgg caagccctgc ggtctgttca ttccagccaa
     5701 tccagacagg ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat
     5761 cgagaaaacg ttgcgccaga taccgtggta aatagccgca gccaaggccg ctaatttgca
     5821 gctccagttt ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg
     5881 aggccgtaga agcgcctgcc tgcgcagtgt cccccaccac aatcgcccga tatcgtttaa
     5941 tgatacgacg gtttaacggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa
     6001 agacttgttc aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat
     6061 gcatcgcgcc aacgataata tccgctcgcc cctgagtcag ggcatcccag gaaccaccga
     6121 gtacgccatt gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga
     6181 gaggcgcaag cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc
     6241 agccttcatg cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc
     6301 gccctttctc taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctacgat
     6361 caagcaattg aatatttagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg
     6421 gcgtcttgta gagtttggct gacgccgccg caaaactgcc ctctttttct atggcatcga
     6481 ggatcatcaa cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc
     6541 ctgctggcgt gatcgtttac tccaacggca ttaccagcgg atcgggatac tggtattcaa
     6601 atcccagttc attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac
     6661 tatttctgaa ctgcggaggt tgcagcccca gtaaacgggc catctgcgga tagaaaacat
     6721 tacgcgcagg gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta
     6781 acaacagagt gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac
     6841 catcaggggc ggtttttccg gcaaagaagc gtcccggatg acgtcccggt ccgaccaggc
     6901 ccgcaagacg cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt
     6961 cttctaatac tcgtccactg ttggttaccg gattacgcgg ggtagtttct ttaaccgtgc
     7021 cttgcgcgtc gccatagaca gatgttgagc tggtaaaaat aattcgggga atacgatggg
     7081 ccagcgcgct atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc
     7141 tacgacgtgc cggaagcgta atgaccagcg catcggcatc cataagggca tccagatcgt
     7201 cagaatcgca aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc
     7261 gggccgcttc gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg
     7321 aaagtgacat cgccagcggc atacctaacc accctaaccc gacaattgcg acctttttca
     7381 tccgttatct cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca
     7441 attcattcta tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa
     7501 tcatcagtaa ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct
     7561 gacaaattat gtttcagggg ttctggtttc cccttacctt caaatggtgt tctgcgggta
     7621 tctttgataa gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga
     7681 taatcatccc atgattcctc agaccagatt agtttcactc a
//
LOCUS       DAEEGA010000145         7554 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEEGA010000145 DAEEGA010000000
VERSION     DAEEGA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.145"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="KLH46_003761"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="KLH46_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="KLH46_003762"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="KLH46_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBE0380281.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="KLH46_003763"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="KLH46_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="KLH46_003764"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="KLH46_003764"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBE0380282.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="KLH46_003765"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="KLH46_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBE0380283.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="KLH46_003766"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="KLH46_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBE0380284.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLAYALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="KLH46_003767"
     CDS             complement(6510..7202)
                     /locus_tag="KLH46_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBE0380285.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1922 a         1828 c         1987 g         1817 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgactatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ctggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgt aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtat tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagagaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc ccgcgccgct gagaaaaagc gaagcgcact gctctttaac aatt
//
LOCUS       DAEEGA010000146         7355 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEEGA010000146 DAEEGA010000000
VERSION     DAEEGA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.147"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(303..2138)
                     /locus_tag="KLH46_003768"
     CDS             complement(303..2138)
                     /locus_tag="KLH46_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380286.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            2239..2526
                     /locus_tag="KLH46_003769"
     CDS             2239..2526
                     /locus_tag="KLH46_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBE0380287.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            2498..4027
                     /locus_tag="KLH46_003770"
     CDS             2498..4027
                     /locus_tag="KLH46_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HBE0380288.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(4197..5414)
                     /locus_tag="KLH46_003771"
     CDS             complement(4197..5414)
                     /locus_tag="KLH46_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0380289.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(5781..6767)
                     /locus_tag="KLH46_003772"
     CDS             complement(5781..6767)
                     /locus_tag="KLH46_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380290.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(6764..7292)
                     /locus_tag="KLH46_003773"
                     /pseudo
     CDS             complement(6764..7292)
                     /locus_tag="KLH46_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         2273 a         1711 c         1370 g         2001 t
ORIGIN      
        1 gattatgtat taatgtcaaa actggcatat tttacagata caatgaacat gcaacccatc
       61 aatctaatag gggcggtatg tacatctgcc catgaatggg atggcgctaa gaatacaaaa
      121 caattagaca tagatcacaa aaacacacca ttctaattga aaaacaaaac aaacaaataa
      181 atcatactat tatagaattt tctcttgcca gacatagata atacccctgc taattttgct
      241 aatggttagc aaaattagca gggtatataa agattattga cccaaaacac actcagatga
      301 attcacgaca gctcaggagg gttattcctg atgtcatctt cacaaataat ggttaacgca
      361 tcatatatca ttctgcgtct gttcctgttt tcggccttat cgccatacaa catcatcaca
      421 tttttaattt catcgaccgg tgatttcaca tagtgaacta atacaccaag cttctcaaca
      481 cattgcagta aagtatattt cttactgcct gtgaaaagcc ctatcatagt ttctttacgt
      541 tcgcgactgg ataatacttt gttttccata atgacttctg ccagcgtctt accatcataa
      601 acatactctt tatctatcag aatatttatc ttatccagca ccttctctac gtcatcaagc
      661 aaacctgtaa tatatggggg cgcaatatat ggtttcgtac tgatcttttc aagcatacaa
      721 ttatacatct taataacacg catcagggta aaattgttcg catcatactg tatttcaccc
      781 tccttatata caggatcaat aagtggtatt ttttcaataa atgcatcctg ataggctttt
      841 acatggtcaa gccaataaaa caacgagcca tactttatat tttttctatt tactttaacc
      901 tccagtgctg tccgaagcaa cgataatgcc cccctgacat atcctgaagg taattcacca
      961 aacaatcctt ttctttccat gtcatcataa tgctgcagca catcattaat tttatttttc
     1021 cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc tggtcacatg aacagcctca
     1081 ttctgcaact gcccaaaata gcttataatc tttttcagat atgcgtcatc acaaccattc
     1141 ttatcattaa tattaaatac aagatctttc agacgggaaa tatattcctg agatttatca
     1201 tcaagtgaaa cactataacg agacaatgtt ccttctgcac tgttttgaaa cttataaaac
     1261 atttccagta atttttttgc ataaggcgtt gtatcaaagc tggtaaagtc ataaagatat
     1321 tgcgcagcgt aaccatacaa accatgaatc cagagtcctt ctctcaccaa cactctgaga
     1381 taatatgcaa ggctttgaag tatcttcgtc tttcgttgta tatcaagtaa gacacttaat
     1441 gaataaaatg cattatagaa tttatcaata ttcctgagca ctttctccag ttcatcctta
     1501 tccagcttct tctcccggta atccttttta tattcatttt tgatagccat gatatattgt
     1561 tgcatcaggc tcttgctttt cccgttatcc ttccgtatag ttcctgtata tttccccaga
     1621 gcatacagct ttccattttc atgaaaatct tccttgttga atcccagttc agccagatat
     1681 tcccgaagat aacgtttaac agcagcttca tcgctttctg tatcagcgtg atcaagacaa
     1741 ggacaatacg atttctgtaa aaaattataa atatgataat aaaaagcaga gtcattctct
     1801 ggtctgtatg tattatacat atctacaaca agaaaaataa ccccaaaagt gcgcagacaa
     1861 tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg gataagaatt catcatgtaa
     1921 taaaaatgtt catatcgggc atatatgatc cgccgttgct cctgactgaa caccccttca
     1981 gcatcaataa tacgactgat ctcattataa cgttgatccc agaatttttg atgcatttca
     2041 aaattggtac tcttccgctt gtgtgccgga ttctcgcctt ccttaaacaa ccccaaaaca
     2101 gtacaaatat catacaacat atttgtaaat gtgcgcatat gctgtgctct ccggttggtt
     2161 tgcattttat cgcagaggac attctgagct taacgtatca aactgacaca acgacatgca
     2221 atgcatatgg agaacaatat gtcaaatcaa accggacagc tttacacgcc ttcacgcatc
     2281 ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa gatctactat ttacgtgaaa
     2341 ataaatccgg cctcgaaata ccacgaccct acctttccca aaccaataag gcttggtagt
     2401 gcatccgtag gctggcgggc atctgccatc gatgagtggc tgatgttaca tacggcacca
     2461 gcctggtctg aacctgaaaa caaactaaag gaacttaatg atgacgacaa aactatcact
     2521 ccctgaactt gacacaacta aactgcctga caggtgccag gcattacttg atgaaatgca
     2581 tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt atgctgacgg tcatggctgc
     2641 ctcggtccag gatacgcatg aagttgaact ttccggaggg caacgtacca gccttcagat
     2701 atttatgtgt ctttcatcag catcaggcag tggaaaaacc tctgcctgcg caaaattaat
     2761 cgctcctgtt catgaaacag aagaagaact acatcaggca tatatcgacg ataaaaaaaa
     2821 ctatgaccga atgatggaaa tgtggacaac agataaaaaa atcctggagc ggagatataa
     2881 aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca cgagcagcac ttgaaaagtg
     2941 tatagcaaat aaacccgaac ctcccgtaca acaggttctc atcgtgaatg acgcgacacc
     3001 agaagggata gctttgaaac tcagccagtc accctctctt ctgttgctgt ctgatgaagg
     3061 aggtactatt ctggataagc gtttcgaacg caaatcggca ctgtacaaca cattatggag
     3121 tgggcaacca gtcaacgttg aacgggcatc cagacccggg ttccggctga atgatgtccg
     3181 tctcactata cttatactga ctcaaccggt aatatttaat aagtttttta ctctcactgg
     3241 cgatcagatc cggggcaatg gctttctggc ccggttgcta ttttgcgaac ccggagcaat
     3301 taaaataatg acgacagaac cgcatactgc cactcctgtt gtgacgcaac aatgtgcaag
     3361 ctgttttcgt aagaaaagtt ttggcagtca gatcagagac tctctgagag catccagaga
     3421 aagacgtgca aaaagtaaag agcgcatctg catgacatta tcaaatgcag catcccgcgc
     3481 actggagcgc tttcacaaag aaaatatgga tactgtcaga cagaatcctc atatgggtac
     3541 atttgaagac attattgtca gaaaaagaga gcaggttgtc aggatagcag cacttctgga
     3601 actggagaaa gatccagact cgacagtaat aacgctggaa agtactaata gcgccattta
     3661 tctcattgaa ttttatttca agcatcttat atataaactt gaatcccttc gggaaatatc
     3721 cccggctgag aaacttgata aatggcttca gaaaagaatt atcacaacag caggttacat
     3781 attccagaaa agttatattt tacaatatgc cccatatgct ctcaggaaaa aatgtgttct
     3841 ggatgaagca cttgacatac tggcggaaca aaggaaaata aggattgatg ataacctggt
     3901 tgtttatata ggaaacacga ttacaccatc cgaactggca aaaaaactaa atattcctgc
     3961 attcgatgcc ggagttttca tttgtgatca tcaaaacata ctgaagtatc accgaaacag
     4021 actatagata ctatcggaca aaaaataaat tatcacaagt gtattattcg ttataataat
     4081 gaataatcgt tatctgagtg caatgccagg ttttcattac gaactggcat tgcatttatt
     4141 tttcaaaaaa gaaagcagta accatttggc tactctgagt atgccagtcc atgaaactaa
     4201 gcgaattcct tagtgttttt agcaagttga gacatcacat aatccgccca tgcctgcatc
     4261 atcggtacac gctgctccag tagatcagta cgatgatatg ccgcctcaac cttatttttc
     4321 agcgtatgag cgagcgccct ttccgccaga tcccgcgaat acccctgttc actacaccag
     4381 tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc gccccggaat gtcactgacg
     4441 gctttctttt tacgcagaaa acttgtcaac accatatcgg aaaggatctg ctgctttctg
     4501 ggtgaaggga acaccagttc atcatgcagg ccacgtatat tttccagaat gtaaatagcc
     4561 tgccgggata aaggaacacg atgctgtagc ctagctttca ttctttctgc aggtatagtc
     4621 catacccgct tatgaaaatc aatttcagcc cagcgcattc ccctggcttc gcccgagcga
     4681 gttgctgtaa gtatcaccat taataacagt gcgcgggtaa cattataagg ttcatcacta
     4741 tacacactgg tcgccacaaa aagcggtaac tgcctccagg gcattgcggg ttggtgttca
     4801 tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca catcaacagg atttgccaca
     4861 caaaaaccgt gcgcccatcc ccactgcata acaacatgaa tgcgctgttt aacccggctt
     4921 gccgtttctg acaaggttaa ccagactgga cgcagtgttt ctgccacgtc cgcagccgta
     4981 atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt aattctcaag cgtcgataac
     5041 cactgcctta catgctttgg attctcccat ccaggagaca gttctgcatg tacacgcctg
     5101 gctgcatcgg caaatgttgg gataacgact ttctcagatt cagccttttt aatctccaga
     5161 ggatcatcac ctgcagcaag ttgctctcgc attatccggg cagtacgtgc agcttcagca
     5221 atactgacct ctgggtaagt tcccaatcca gcattacgtc ttttttgtgt caccggactt
     5281 acataacgaa aaacccattt cccccgcccc tttactgaag aaggatgaag ggtcagtccg
     5341 gtaattcccc catggggcaa tggtttgtca tcaggtttga tatgtcttgc tttcgtatcc
     5401 gtcaatactg ccatacgcta attcccgtca ctctggtatg ccatccagta tgccattagt
     5461 ccctcgcttc actaagaata cattagataa catcggacaa caaaaagtgt aatacattgt
     5521 ttataaagaa attatataac acccctggat taagtcagat ttatttcagg cggtccccct
     5581 caccgccata tttaaagaag agcgcgtacg aaagtacggg cttttttttc gtatattgta
     5641 cacaccgggt gcccccgccc acgtctgtta gggcgagggg aaattgtgca catcaactgt
     5701 ctggttactc agtcaacaat caaaatgcgt aagatagtac agaacatata tgcgttatct
     5761 ggtcacttcc tgtaacttcc tcacttatta aaatgccccc aacggaaata catgtcacat
     5821 gccatcataa aatcgttatg ccagaggaat cgacaatgga aaaaatagta aattaagcac
     5881 ggtacatagc agagtaaaaa ataggcttca aaccaaatcc gccattccac aagcgttgcc
     5941 aataccgaca ccagcgtaat ccacatcatt tcaataaccc gcaatatgaa taaccgaagc
     6001 caaagcggct tggcaaaaaa ggtcgacaac gtattttcac tggagaaaaa cagatcgatt
     6061 tcctgcaaaa aaaacctgcc cagaaaagcg ggccgcttgg agaagcgcag aaaaaacaga
     6121 ttaatcgcca acactacgcc acaaagaatg agcaatgcaa ttatcgattt aaacgttaac
     6181 cactgcccct ctgtcacaaa agaatcactg ttacgcaacg ggctgatagt aagcaggtta
     6241 acaaccgttg agatgcacac cgaaagcagc acatagcgag tcagactttt atattttact
     6301 tttttcgcta ctgaaatagc gacctgcttg acataactgg tgcggatcat ccgaaacagc
     6361 agaaagatga ccaacggccc aagtaacgaa atcaggaaca gcgcatataa cgagggtaga
     6421 agctggtata gcccccaact cattgcactc aacatgatta acgacagaaa tcctgccacc
     6481 agaggttgtg taagaaataa ttctattttg tggttctgct ttttcttaaa ccacgaaaaa
     6541 tcgatattct tcgtgacgca tttataagcc cagtacgcct tgagatcaaa aataaaatta
     6601 tacagcacca gggccagaat cccactaact acaccaatat cctctgatgg aaaagtaaac
     6661 cctgcatttt tccaataaaa gcctatcaga ataagtccaa tagcataaat gtatatcaga
     6721 tttcgtttat cctgaaagcg aataaaagtt aaataatcag gaatcatttg aatacctttg
     6781 catcactaaa tgctttacga caatgcataa ctcgttgctg atccacgcca gcaaaatcat
     6841 ttaacatatt ccccaaatag ggcttattac ttatttcaag gaaagctacc ggggttggca
     6901 tattcagaat atgtatcgag agattcagtc catttcaccg tatgagttaa gtgtaatgag
     6961 agattttctt tgataattga cggagcgacc tcgctttttg ctgttacgtt catcagaacc
     7021 tgatgctctg gcgaagcgat atccagtccg gcaagataat cgcgcattgc ctgaacgccg
     7081 tcttccatca aacgcgtgtg ccaggcaccg ctcacaccga gtttaaccgg ttcgtatcca
     7141 gcggccatca gcagcgtggt aaattcattc aacgcagcct gcgttccccc aataacctgc
     7201 tggcgcggcg tgttatcaca gctaatatcc agcgcaatgc ctgattccgt aatcatcgtc
     7261 tgcagttgtt cgcggttgat gcctttaacc gcctgatctt acccagcaat agtggacacg
     7321 cggctaagtg agtaaactct cagtcagagg tgact
//
LOCUS       DAEEGA010000147         7183 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEEGA010000147 DAEEGA010000000
VERSION     DAEEGA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.148"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(296..1198)
                     /locus_tag="KLH46_003774"
     CDS             complement(296..1198)
                     /locus_tag="KLH46_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBE0380291.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="KLH46_003775"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="KLH46_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HBE0380292.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="KLH46_003776"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="KLH46_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HBE0380293.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     DLSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="KLH46_003777"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="KLH46_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HBE0380294.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="KLH46_003778"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="KLH46_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HBE0380295.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="KLH46_003779"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="KLH46_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="KLH46_003780"
     CDS             7090..>7183
                     /locus_tag="KLH46_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HBE0380296.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1673 a         1811 c         1878 g         1821 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatgcccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgacttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc cactctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg ttcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgctctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAEEGA010000148         7142 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEEGA010000148 DAEEGA010000000
VERSION     DAEEGA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.149"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(13..706)
                     /gene="ospZ"
                     /locus_tag="KLH46_003781"
                     /pseudo
     CDS             complement(13..706)
                     /gene="ospZ"
                     /locus_tag="KLH46_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            762..914
                     /locus_tag="KLH46_003782"
                     /pseudo
     CDS             762..914
                     /locus_tag="KLH46_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2295..3242
                     /gene="icsP"
                     /locus_tag="KLH46_003783"
     CDS             2295..3242
                     /gene="icsP"
                     /locus_tag="KLH46_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HBE0380297.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3804..4176)
                     /locus_tag="KLH46_003784"
                     /pseudo
     CDS             complement(3804..4176)
                     /locus_tag="KLH46_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4420..4539
                     /locus_tag="KLH46_003785"
                     /pseudo
     CDS             4420..4539
                     /locus_tag="KLH46_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4789..5655
                     /gene="ospB"
                     /locus_tag="KLH46_003786"
     CDS             4789..5655
                     /gene="ospB"
                     /locus_tag="KLH46_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HBE0380298.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5984..6724
                     /gene="phoN2"
                     /locus_tag="KLH46_003787"
     CDS             5984..6724
                     /gene="phoN2"
                     /locus_tag="KLH46_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HBE0380299.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
BASE COUNT         2241 a         1344 c         1382 g         2175 t
ORIGIN      
        1 aattaatata cattaagtaa caggcattcg agccttgatt tgagaaagcc tgtcattcat
       61 ttgttttatg tagctatcta tttcctctga tgcataacta aaaagttctc ccatttcacg
      121 agcaataata atctcagatt taatagactt taatctctgg cgactcatct cttaacactt
      181 gcataatctc tctttttata tcatatagtt ctataaaccg atttgcccgt agttctcctt
      241 ggggagaatc atttctgaat acaacaatta tatacaaaaa atcactattt tctttagaga
      301 agatcttaca actaatctct gggagctcac ataatgcatg taagtcagga tggtacaaac
      361 tatccacaga aaattgcccc ataggacgtt ttccagatat aactgtcaac cagcttcctt
      421 ccggtgtact ggcttcggct ctggaaatag gctgaaaatc ttccggacat ggagaataca
      481 ttgtcttcat ataattaagt cgtctggatt tctcattaat aaactcctct atttttaatt
      541 gtgccatact tctggttata ccagagatat acttcttgtc atatatgttt ggtgattcat
      601 taaagtaacc atgttcaact ctaggataaa tatttctcac tatatattca gtatttgcag
      661 tatttattgg aaatacgttc ttaatattct tgatgggact aatcatttta atctctatac
      721 tcaaatataa acattaccat gaacaatata attgttccta agtggaatgt ctccacggag
      781 aacactttat ctatcgggaa atagtgcggg caacagtgtt taattatatc gagtgcaatt
      841 acaatcgctg gcgacggcac cgttggtgtg gcggcctcag tccggaacaa tttgaaaacc
      901 agaacctcgc ttaggcctgt gtccatatta cgtgggtagg ctcacagagc ctcacttacg
      961 actgcgtcca tattatgtgg gtaggatcaa taaagacgtt ttatatgtgt ctgcttgcag
     1021 ccagaaaatt tataccctgc aggtatcata aacatgcttg ataacttaat tggggctccc
     1081 tcttcctttt atggtgtaat tgtttgattg aatacttccg gggatttcag tatgaaatga
     1141 agtatattta atatacttta caaaaagttt gtattagtgg aataaggaca atagcttatc
     1201 caaactgcca agcctcttta ttataagtaa gatctggcac tggaatattc tcaaaaggac
     1261 taacatcatc accacgacct tcaaccaacc attttattgc atttaaatcc cagaatagtt
     1321 cagtagtggt caatttggga aacgcacaat attgtacggt tacgttatcg caatagagtg
     1381 aactctcgcc ctatacggcg ttttcaagga ttagtcctta atcggacaac cacattaaag
     1441 cataataaat aattaagctt taataatata ctatagatac agttctatta taaaaaatac
     1501 tattttaggt gatgcattaa attagtagaa tatttattct tttataaaag caccaatgaa
     1561 ttgtccaagt aaacatgaga taaaatgtgt agaggataac tgagtttggt cactttaact
     1621 gtattagtcg cagtctgact ctcgacttta aaaggatggg aattacctgg actgatagtg
     1681 agaaagcttt ccattgaaat aagtcaaata aaagttgatg gcttcagtgc taaagtgagg
     1741 tgcccgctag ccgtattcac tcgaatcgga attcaatgta ttgcttctgc catttccata
     1801 atatttcacg ggaaaaacaa aatctgaaaa actatactta agtgttgtat tgagtcagtt
     1861 gagatacaaa tacatattta tcagcacacg ttacgtcaaa tacaggaggt tgtcatgttg
     1921 gtactgaaag gcacgttggc tattgcagcc gtgatgacaa tttattgtct tatcgttgtt
     1981 cttatggaac gcttttctga ttgatttcat attggcgaga gaacaagaag gtgagtagaa
     2041 ttgtcgcgga atcctgaaac tatcagcctc aggtatgaac accaaaaggt aggtagagaa
     2101 atctatggcc cccgttactt tattcgcaca atgaggtatt tttttatttt tacaaaagtt
     2161 caataaatat tataataaaa gatatgttct tggtaacata aacaaatatt taattaaaat
     2221 aatgcttggc attgacgttt atcttataaa gtaagaagat catggacatt tcaaccaaaa
     2281 aagtagagtt ctcgatgaaa ttaaaattct ttgtacttgc actttgtgta cctgcgatct
     2341 ttactacaca tgctaccact aactatccac ttttcatacc ggacaacatc agtacggata
     2401 ttagtctagg atctctgagt ggcaaaacaa aagaacgcgt ttatcatccc aaggaagggg
     2461 ggagaaaaat tagtcaactg gactggaaat acagtaatgc gactattgtt agaggtggca
     2521 tcgattggaa gctaattcca aaagtgtctt tcggagtttc cggttggact actttaggta
     2581 accagaaagc aagcatggtt gataaagact ggaacaattc caatacccct caggtatgga
     2641 ccgaccagag ctggcatccc aatacgcatc tccgtgatgc taacgaattc gagctgaatc
     2701 ttaaaggttg gttattaaat aatttggatt atcgacttgg actaatagca ggttaccaag
     2761 agagtcgtta cagttttaat gcaatgggag ggagttatat ttatagtgaa aacgggggga
     2821 gcaggaataa aaaaggggca catcctagtg gtgaaagaac aataggttac aaacagctct
     2881 tcaaaatacc ttatattgga ttaactgcta attaccgcca tgagaatttt gagtttggag
     2941 cagaactgaa atatagtggt tgggttcttt catctgatac agataaacac tatcagactg
     3001 agacaatttt taaagatgaa ataaaaaacc aaaattactg ctctgttgct gcgaatattg
     3061 gatactatgt cacccccagt gcaaaatttt atatagaagg ctccagaaat tacatttcta
     3121 ataaaaaagg tgatacatct ctttatgagc aaagtaccaa tatatctggc accattaaaa
     3181 atagtgcaag tattgaatat attggttttc tcacttccgc aggtataaag tatatttttt
     3241 gaattggttc accgagttga ttacgttttc atggctaaaa attgccgccc attgtgagtc
     3301 atattcgccc tgactttcca gaaccatacg aactgcccgt tgacggactt cgggggaaaa
     3361 acgtgtattt ttagtcattc tgtttacctc tttctcaggg cgtttagtct ccaggatttc
     3421 cggggcggtt caaaaaactc ttcgttgatt actgtggact tactgttggc attaccgacc
     3481 ctgagaccgg agaaataaga accgcttaga tcatcgtggc tgttctcggg gtatcaagtt
     3541 acacatgggc agaggctacc caaggtctac tattatcgcc tgaaagcgct gatggaacag
     3601 tgctatcagg gacatgctga tgaaaatacc taaataatca gccagcactc tatctttcca
     3661 aatccacagc atagcaaaga gagcaaaaga gcctgtaaat tcagaaatta aggaaacgcc
     3721 tcttggtgta cataatctat cttatgttaa atgaaatcta atttcttgat tatatgctgg
     3781 atcgttcgtg ggtggctttg cccctatatt tccagacacc tgttatcatt ggcccgctgc
     3841 cgcagatatt cccgtggcga gcgataatcc agcgcactat gcggatgcca ttcgttataa
     3901 tgctcgaatg cctctgcaag gttctttgct gccgttaacc cgtcaggttt gggcatgata
     3961 ctgatgtagt catactttat tgttttcacg aagctctttg ctattctgtt actctccgga
     4021 ctccgcaccg ccgtgttctt cggttcaaca aagtcccaac atccgggtga actggtgtgt
     4081 ttcattagct cggtagcatg aaccattatc cgtcagtcac ttcactggag acgccggaag
     4141 ctcgttgccg aagcggcgtt ccaccgctcc cagcatgtcg ccaacgggag agcatctacg
     4201 gtctgattga actgtttatt caaattgtcc accgtctgtc agtacgctct gaacgtcggc
     4261 tggttaaaac actgctcgct gattttcaga gggttcacgg taaaacagca cttttattcc
     4321 ggattgcgga agcggctctg aataatccgg atgggctggt taaagaggtt gtgtaccatc
     4381 tgcatattgt tgaaccagat cgcagcggta agagaagttc atcatacttg gcgcagttac
     4441 gcgatgtttc tgctagaggt gatgccgtaa agaacggcag aacattaccg gaacaagata
     4501 gcggtctacc tgcactggta tcagacccgg ggcttccccg gatgatctcc acggtacttt
     4561 aggtactgtg tctatattat gtaggtagga tcaattaact ttgtagttga aattctttca
     4621 ttgaaataat gaataaaaaa gacaaaccgc attaaacaat gccagaatat aattgcattt
     4681 ttgttccgtt tttttaaatc atagtataca aagaagcttg catactatat gaaaatataa
     4741 caccctttac tatgatgttt ataaaacaat atatggagtc atgtaggtat gaatttagat
     4801 ggtgttagac catactgtag aatagtcaat aaaaagaatg aaagcatatc agatattgca
     4861 tttgcacata taataaaaag ggtaaaaaat tcatcatgta ctcacccaaa agcagcattg
     4921 gtttttttag gagagaaagg tttttgtgat agcaatgatg ttctatctat tatgggacaa
     4981 caaataccaa gagtatttaa gaacaagatg ttatatgatt atgtttttaa aaatgaaaaa
     5041 agtaaaaatg attttctaaa aatggctgaa tcatggctac cacagagtga accaatagta
     5101 ataaataatg atgatgacgc attgaatgct gctgcttatt tttctgtaaa aaaagcgaaa
     5161 ataaaaacag taaacgatac tgattttaaa gagtataata aggtttatat tcttgggcac
     5221 ggtagtcctg gttctcatca attaggcctt ggttcggaac ttattgatgt acaaacaatc
     5281 atttcaagaa tgaaagactg tggtattcta aatgtgaaag atatccgttt tacttcatgc
     5341 ggctccgctg ataaagtggc tcctaaaaat tttaacaatg cccctgctga aagtctttct
     5401 tgtatcctta actctctgcc tttttttaag gaaaaagaat ctttgctaga gcagataaaa
     5461 aaacaccttg aaaacgatga gtcattgagt gatggtctaa aaatatccgg ctatcatgga
     5521 tatggagttc actatggtca agagcttttt ccctactcac attatcgttc aacttcaatt
     5581 cctgctgatc cggagcatac agtaaaaaga agctctcaga aaaagacttt tattattaat
     5641 aaagaactgg attagtataa aatttttaac ctatagttaa ttgtttgcat ttaagatgaa
     5701 gttattttat ctttagtaga aaagtgcttc cctatgaggg aaaatcacaa atcatcataa
     5761 tacagagaca aaacgatacg aaaaaataga taaaaaacat cattctttta ccacattatt
     5821 ttccgtgaat atgaaaaata acgttattac tttaatataa gactattttt tgtttttcca
     5881 tcactctgtt caaatttttc cgcatgactt gtgttttttg taatacagct cgttttttac
     5941 agctgaccaa aatcatcaat taattatgct aaggaaataa attatgaaaa ccaaaaactt
     6001 tcttcttttt tgtattgcta caaatatgat ttttatcccc tcagcaaatg ctctgaaggc
     6061 agaaggtttt ctcactcaac aaacttcacc agacagtttg tcaatacttc cgccgcctcc
     6121 ggcagaggat tcagtagtat ttcaggctga caaagctcat tatgaattcg gccgctcgct
     6181 ccgggatgct aatcgtgtac gtctcgctag cgaagatgca tactacgaga attttggtct
     6241 tgcattttca gatgcttatg gcatggatat ttcaagggaa aataccccaa tcttatatca
     6301 gttgttaaca caagtactac aggatagcca tgattacgcc gtgcgtaacg ccaaagaata
     6361 ttataaaaga gttcgtccat tcgttattta taaagacgca acctgtacac ctgataaaga
     6421 tgagaaaatg gctatcactg gctcttatcc ctctggtcat gcatcctttg gttgggcagt
     6481 agcactgata cttgcggaga ttaatcctca acgtaaagcg gaaatacttc gacgtggata
     6541 tgagtttgga gaaagtcggg tcatctgcgg tgcgcattgg caaagcgatg tagaggctgg
     6601 gcgtttaatg ggagcatcgg ttgttgcagt acttcataat acacctgaat ttaccaaaag
     6661 ccttagcgaa gccaaaaaag agtttgaaga attaaatact cctaccaatg aactgacccc
     6721 ataaagctgg acagcctgta tcaggctatg gagggcccat agacaaatct accctatatg
     6781 agcataggag gagtctatgg gcacaccacg ttttacccct gaatttaagg gattactgga
     6841 aaggctggga catatccctc cggcagaagc agaaaaagct tattatgctg ccatcggaaa
     6901 cgatgatctg gcaacctgag ttcacagata aaacattctc taggaaactc ggggcggttc
     6961 cgttcaccac atgcaatgtg gtgttgcagg ggaacggtct gcaccatccc ctatgtcgat
     7021 caatataaca gaaatgacaa cttcagattc agggcacaac ctaaatatat tttaggtcac
     7081 ctctcaaatc gtttgcctga taccgctcca ttttttaata aaaaaatcaa tcatttttga
     7141 at
//
LOCUS       DAEEGA010000149         7017 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEEGA010000149 DAEEGA010000000
VERSION     DAEEGA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.150"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..695
                     /locus_tag="KLH46_003788"
     CDS             <1..695
                     /locus_tag="KLH46_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBE0380300.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1034..1804)
                     /locus_tag="KLH46_003789"
     CDS             complement(1034..1804)
                     /locus_tag="KLH46_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380301.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1849..2283)
                     /locus_tag="KLH46_003790"
                     /pseudo
     CDS             complement(1849..2283)
                     /locus_tag="KLH46_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2295..2516)
                     /locus_tag="KLH46_003791"
     CDS             complement(2295..2516)
                     /locus_tag="KLH46_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380302.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2517..3200)
                     /locus_tag="KLH46_003792"
     CDS             complement(2517..3200)
                     /locus_tag="KLH46_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBE0380303.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3264..3575)
                     /locus_tag="KLH46_003793"
     CDS             complement(3264..3575)
                     /locus_tag="KLH46_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380304.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3572..4474)
                     /locus_tag="KLH46_003794"
     CDS             complement(3572..4474)
                     /locus_tag="KLH46_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBE0380305.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4914..5873
                     /locus_tag="KLH46_003795"
     CDS             4914..5873
                     /locus_tag="KLH46_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBE0380306.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5873..6268
                     /locus_tag="KLH46_003796"
     CDS             5873..6268
                     /locus_tag="KLH46_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBE0380307.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6451..6831
                     /locus_tag="KLH46_003797"
     CDS             6451..6831
                     /locus_tag="KLH46_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBE0380308.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQHITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6877..>7017
                     /locus_tag="KLH46_003798"
     CDS             6877..>7017
                     /locus_tag="KLH46_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0380309.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCS"
BASE COUNT         1910 a         1822 c         1700 g         1585 t
ORIGIN      
        1 ttgatgcaga agccatatgt gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
       61 gaactgagac ccagcaagca ataagggcat tacaccgggt cagagaatcg ctcatcagag
      121 ataaggtgaa aacaaccaac cagatccacg gtttcctgct ggaatttgga atcagcctgc
      181 cgacagggga tgccgtaata aaacggttgt ctctcgtatt ggctgaacac gaaatccccg
      241 aatatctgag ccgccttttg gtgaggttgc atacccacta cctttacctt gttgagcaaa
      301 tagcagagct ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca
      361 ttatgacaat tcctggtgtt gggccgatta ctgccagctt actatcgtcg caacttggtg
      421 acgggaaaca gttttcatgc agcagagatt ttgcggcttc cacagggctc gttccccggc
      481 agtatagcac tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt
      541 tacgccggtt actggttcag tgtgcccgct cattcatgat gcagcttgaa cgccagcatg
      601 gaaaacttgc tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttgcct
      661 gcgctctggc aaataaactg gcccgaattg cctaggcaat cactactcag caaaaagaat
      721 atcaggcctg atatatagat gctgacaaaa ctcaattagc ttattgcaaa caataagata
      781 caagttaccg tctggttttg cgaagattga ttattgatga catgaacggc aaatcgacct
      841 gattaaaaac ctaacagggg aaaatggctc actgaagccg aggggatttt taggattatc
      901 aggtgcggaa ctcatcgagg cgcgggcatc ccttgcccac agagacgccg gatagattta
      961 agcaagccca ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc atagattttc
     1021 cccgccatcg tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg
     1081 cccgggcttc ccggacaact tcccgcagaa caccacggtc aaaccaccgg cggggcggtt
     1141 catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat
     1201 cgctgaccag atacatttcc accgccgaaa aacgtgcgcc ccggttcagg atggcatcga
     1261 gcaccacggg caggttttca tcgtcttcat cgagccagca ggggtgatcg ctttccttca
     1321 cgaccaggcg aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg
     1381 ccgcccggat acgccgcgca tcttctgccc cccggcaact gacactgcat actgcatcgc
     1441 gcaccgcatc aagaactgcc ctgatttcgt cttcggtcag ccattcaccg gcatacggga
     1501 aagacacctg cgttccgtcc gccggaagta ccggcagcga ctgccactga ctgccggaca
     1561 gaacaagttc cgctccggtt ccggtcagtt tctgaatggc ggaggccagg tgcggcggca
     1621 gcacgccatc gtggcggtta acgagtaacg tcgccagccg gggcgcatcc agccgcacgt
     1681 aaatgctgat ccagcccgga tagttcgggt tacattggtc aacatccgtc tcttccggcc
     1741 agccctgcgc cagcagcagg ttacgggcgt gacgacaaat cagagcattc agcgtttcat
     1801 tcataattca tgaactccct gtatcgccgc ccacaggcgg cgataacatc agtcgttttc
     1861 gtgtgtgttg tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc ggcgggtaac
     1921 gtattccggg agggggaaac cgtgatcccg ggcagcccat tcacgtttcc acagtgcctg
     1981 ttcagcgatc tgagtcaggg tctccagcag ttcagcaggg acggtcacct ggcggttttc
     2041 gcgcacgttg ctgataatct ccatcgccgc ggcggaagaa ataccggact caatccgcta
     2101 gtcttccggg atgtcctcca ggtgcgccag gacggtcctg atttcatgat cggacaggga
     2161 aatatccatt ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag
     2221 cggcgtgtct cccgaatact ttgctgccag cgcccggcat agcgtttcgc atgttccgta
     2281 catactctct cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc
     2341 tccatcaaga ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc cggcagggtg
     2401 tcagcattaa agctgtaccg ccgctccatc tcattcatgg ttgtacgcag gtcgcacatg
     2461 gcattggcca cctccgccac ttcggcgcga agtttttcgt gtccggcata gttcatttac
     2521 gcagcctccg gcataaacca gtcatcattc gcggccccct gctgcatggc ccgctgtacg
     2581 gcagccagtc gttgctgtcc ggcacggtga tactgctcaa gcagctcgat accgatatac
     2641 cggcgtccgg actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa cgggtccagc
     2701 actatcgcat tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt
     2761 tccgtcgggt gatggcggtt gcccgaatat ttccagccca acacgtccgg cagcgggttt
     2821 tgtggcagac gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca
     2881 tatgcggcct tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc
     2941 gcatttttcc aggcagacat aaagcgatcg acgcggttcc agccatagaa gctcaccatc
     3001 agcgcgtctt ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatca
     3061 gttttatcac cagcaatggt acgcccctga cggtcacgaa aaccgacgag atatggcgga
     3121 tcggtgagga tgaaatcaat ggcgttatca ggaatacggg ccataacatc gatgcagtta
     3181 ccgaggacaa aacgggacat aacagatcct tctcttacag agggcacccc tgtggtgccc
     3241 gttccccgct gcctgtgggc tgttcatcgc ttccggtaac gggtcaggcc gaccgtggaa
     3301 taccgcagcc gtcgtctttt tgacagcaag gttatgccgc gaaagcggct tgaagggcgt
     3361 tcatcagaaa agggcgacgg gctggcgtac actgtcaccg acgccagccg ggagcggtaa
     3421 aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc
     3481 gccgcgtcag ccggcgcagt aaaaagagga gcacggcgac atcctttgtc ggaaaagaaa
     3541 cgggcggttg cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga
     3601 ctatccacgc aggtccggtg acttccggct gctcatcgtc atctgtcggg gaagaccatt
     3661 caagttcccc ccacagcaca tcgacgagct ctccacgctc atacaactgc cagccacaga
     3721 aatcacagcc ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt
     3781 ctgcaataac gtcgctttcc ggctgacacc acggagtgtc gaaatccacc tgaatgaagt
     3841 tttctccctg actgctgata ttcaggtcat atccacaagg ccacttcacg ccataccgtt
     3901 ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccatta aagccgttca
     3961 cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg
     4021 gcactacgtt gtcacacaga cggttccacc acaccgatac atcctcgtta ccccatatgt
     4081 cacaccagtc acctcttttt gcggtgaaat gcacagtgat ggtttccctg gcatcatccg
     4141 gtaatccttc ccaacgacgc tggccagtac cgctctgtaa ccagagttca tgcagcatca
     4201 ggcagttttg ttcatccagc aacacaccgt tctgcaggtg tgtcagccag cgggtgaacg
     4261 cgatattttc cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag
     4321 agcggatatt ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
     4381 cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct
     4441 cagcgatctg tgccggttca ccaggaaaat acatacgatt cgagcaccag tttggcatgg
     4501 gaattctcct ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg
     4561 catacgaatg caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg acgaaacgtg
     4621 accgggtgag gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg
     4681 ccggcacggc ctggttaaat agtatccgtc tcagttcgtg gcgcaaaatg agaaaccaat
     4741 aagaactcaa aatgaaccac aacaagaaca taaagagact ctaaaagagc aacaccgttt
     4801 gacatagact caaaatgagt cacaataaga aacaataaaa aactcaaaaa gatgcaccga
     4861 cataaaggat gctcaatttg agataaacac aaactcaaaa cgaggcaaaa agaatgctaa
     4921 aagtatcatg cgatgatggt tcaactaacg taaaactggc ctggcttgaa gatggcgagg
     4981 taagaaccag tctgtcagga aacagcttta aagaaggctg gaacccggga ctgtttaatg
     5041 ccggcaaagt ttacaattac gtcgtggatg aaaaaaaata cacctatgat ttgggaagca
     5101 ctgctgtcat aggtacaaca catgtcagtt atcaatacag caccaccaat ctgcttgcca
     5161 tacatcatgc actgctgaca tccggccttc agccacagga tgtagaactg acagtaacat
     5221 tacctgtgac cgaattcttt gataatgaca atcaaccaaa tgaagaaaga atagaaagaa
     5281 aaaaggcaaa tgttctccgt gagatatctc tgaataaagg ggaaacattt aaaattaaaa
     5341 aagttaatgt aatgcctgaa tctttgccag ctgcatttga atcactaaaa aaagataaag
     5401 taaacaaact tgaacgcagc ctgattatcg atcttggagg aaccactctg gattgtggtt
     5461 taattctcgg tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa atcgggacat
     5521 cccgcatcac tcataccgtc atgaatgcgc tgacgaaagc ctccacacca tgtaattatt
     5581 ttattgctga tgagttaata aaaaacagac acgataatga atatctccag acattaataa
     5641 atgatgtagc tgaaataaaa aatatttctc atgtaataga cagagaagtg aaatctctgg
     5701 cagaaagcat caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg
     5761 gcggaggagc cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga
     5821 acaaggtgga tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag
     5881 cagtgatcca aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca
     5941 ggacaacctg acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta
     6001 cagacaatcc cttatttgcg gagcagctct ttattctgtt gccccccgac tgttaacgct
     6061 aatcagcgtt tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac ttatagaaca
     6121 aacaacagga tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc
     6181 atcttcagaa aacaaatcag agagcatcac ttcaaaagat gatttcgaag aacaaaccag
     6241 acgcaatctt tctatgttaa aaaaataata aaagaagagc aggttcagtc aattacctgc
     6301 tcttctttta tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa
     6361 tgcaacagac aggaatgcaa taattttgca ttatctcgtt gcttgtaatg caaatatgca
     6421 ttattgtcag tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt
     6481 gatgatgata taaagaacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact
     6541 caggctattg cagcttttta tcagcatatt actgaacaaa agaaactgcc atttgtgatc
     6601 acctcaattg taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa
     6661 gctcttgcag tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa
     6721 gcaaagctaa tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa
     6781 ataggtcttc tttcagataa ccgggatgct gaactaggct gtgtccctta atcatctgag
     6841 ttatagtaac tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat
     6901 gaggcatgga ccatcatcaa gcccctgtta cctcctgaac ccgccacacc acgggccgga
     6961 cgcccatggg ctgagcaccg taaaatcatc aatggcatgt tctgggtgtt atgttcc
//
LOCUS       DAEEGA010000150         6859 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEEGA010000150 DAEEGA010000000
VERSION     DAEEGA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.151"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(53..532)
                     /gene="xdhC"
                     /locus_tag="KLH46_003799"
     CDS             complement(53..532)
                     /gene="xdhC"
                     /locus_tag="KLH46_003799"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBE0380310.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(529..1407)
                     /gene="xdhB"
                     /locus_tag="KLH46_003800"
     CDS             complement(529..1407)
                     /gene="xdhB"
                     /locus_tag="KLH46_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBE0380311.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1418..3715)
                     /gene="xdhA"
                     /locus_tag="KLH46_003801"
     CDS             complement(1418..3715)
                     /gene="xdhA"
                     /locus_tag="KLH46_003801"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBE0380312.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIDWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4130..4885
                     /locus_tag="KLH46_003802"
     CDS             4130..4885
                     /locus_tag="KLH46_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBE0380313.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4964..5037
                     /locus_tag="KLH46_003803"
     tRNA            4964..5037
                     /locus_tag="KLH46_003803"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4996..4998,aa:Gly,seq:ccc)
     gene            5152..6756
                     /locus_tag="KLH46_003804"
     CDS             5152..6756
                     /locus_tag="KLH46_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE0380314.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSVSLRT
                     KDRRTAMAHSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDR
                     FNDIDRQDDQADSVSLSVSAPEVKPEVTPSITIASLFEQYEAENSQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLKVRDVLQQLPKNRKQRFKDAPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT         1708 a         1687 c         1789 g         1675 t
ORIGIN      
        1 gttaaacaca taacctcagt gaatccggcc tgaattcagg ccggatatcc ttttacttcg
       61 ttttctcgca atccagaact gtatttacaa tcatctgata ccccgtgcag cgacaaagat
      121 ttcccgccag tccgcgacga atttccgtaa tggttaatgg cttctcgcgt ggtttcgcca
      181 gcattgccgt ggtagccata atcaggccag gcgtacaaaa cccgcactgc actgtgccgg
      241 atttcgcata agcctgctga acatgagaaa gttttccgcc tttcgcttca ccttccagcg
      301 tgcggatctc ttttccttca gcccaggcgg caaggtataa gcaactgtct attgctgtgc
      361 cgtcgaccaa caccgtacag gcaccacatt cacccacgca gcacccttgt ttgacactta
      421 gcagtccttg ttcgcggagt aattccgaga gcggcgtgcc tggtgcggcg tgaagctgaa
      481 aaggcatccc gttaatggtg cattcggtgg taattgtttc gctgtgattc attgcaattt
      541 tccccccgcc gcggcgacgg cttcgctaat cacttttttg gtcatcgtct ggatgagatg
      601 cagacgaaac tctttactgg cccgccatga agaacgcggg gcgacatctt gcaggacaga
      661 ttcgctgata gcttccagcg tttgcaggtt taatggcgca ttttgtgcag tctgttcggc
      721 atgttggcag cgaatcggcg ttggcgcggc aacaccaaat gccaggcgta attcgctgaa
      781 attgccgtta tccagtcggc aatgtgcggc gcagccaatc gttgaaatat ccattgcgtc
      841 gcgcatggca tatttaaaat gcgcgctgcc cgcgtgttct ttcggctgtg gcggaaaatg
      901 aaaggcgacg aggatttcgt catgctcaag agacactttg cccggcccgg tgtgaaagcc
      961 attaatcggg acgaaacgaa caccgcgcgg ggagtggatc tccagtttcg cgtcataaat
     1021 tagcgttggc gtggcagaat ctgcgctggt ggcaccgttg caaatatttc caccgtaggt
     1081 agcgacgtta cggatctgcg gtccagcaat gaacgtggcc gcagcacata acgccgggag
     1141 atgacgttga gttataggat cttctattag ctgggtaaat gtcgttgcag agccgatacg
     1201 tagcgagcca tcttccgcca gcgtaattcc ccgcagctcc gccagattat gaatatcaac
     1261 aatatggcga taacggtcat tgtgatggtg gagctgaatc agtacgtcag tgccaccggc
     1321 gagcagtttg gcctgcgggt tgtcagccag caggttgatg gcatcggcaa gggttgctgc
     1381 gcgatggtaa gaagcaaaat caaacatgat gttatcctca aatcaatcct gccagatgga
     1441 actcttcata taaccgtttt ggcgtcagcg gcagtgtatt gattgcaaca ccggtagcca
     1501 tcttcaccgc gttacgaata gcagcggcaa caggaattat gggtggctca cccagtgact
     1561 tatgtccgta tgcggattgc ggctcattga tttcgacgaa cgcgctttcc agttgtggca
     1621 gatccggcat ggtcggcatt ttgtaatcca gcagattggg gttacggacc acgccgcttt
     1681 tagcatcgat gatcatctct tcaaatagcg cccagtcaat gcccattccc attccgccgt
     1741 gtacctgacc ttctgccagc agtggattaa gaatatgccc tgaatcatga acgttgagga
     1801 tgcggttgat ggtgactttg cacagcgcaa tatcgaccgt cagatcaaca aaggtacagc
     1861 caaacgccgg tgggttagtg gtggttttga tggagctttc agcagagagc tgcccgccgc
     1921 gttcagggtg gtagaaagcg tccatcgcca aatcttttaa cgacattaac ggctcttccg
     1981 gtcgttcaac cagcacgata tggcctttta tcagggtcaa attcatcgct gactgatgta
     2041 gcatgactgc ggcgtgagcg atgattttct cttttaataa tagtgccgca ctgcgcagcg
     2101 caggcgcggc aacatagctc tggcgtgagg caaatgcgcc gggatcgaac ggcgtaacgt
     2161 cggtatcttg tgttgaaata acgcgaacgt cgctgaccgg aacccccacg gtttctgcca
     2221 ccatttgcga gaagacggtg tcggcaccct gaccgatttc cgtcgcgccg ctttgcacgt
     2281 tgatggttcc atcctgattc atcagaaggc gcgcgcctgc tatttctacg ccgacaggcc
     2341 aggtgttaga ggtgtagcta aaacaggcga cgccaacgcc gcggcgcaaa ttgccttgct
     2401 ggttctggca ttctgcacgg cgtttttccc attcaaagat tttccggcct ttttcaagac
     2461 actccggcaa ccctgcgctg caaatacgtt tgcccgtgag cggattagca tctccttcgc
     2521 gggcggcgtt gcgtaaacga atttcaacag gatcaatacc taacgctgtc gcggcgtcat
     2581 caagcataga ctcaacggca aatacgactt gtggcgcgcc ataaccacgc atcgcaccag
     2641 ccgaggggag gttggtatag caggtctttg aactgtaagc gtaggcacaa cgaggataaa
     2701 ggtaagcgac tttattcccc cccgcagaag cgatggagtg cccgtgagat gcataagcgc
     2761 cggtgttaga cagaacatcc agactataac ctttcaaggt tccgtcgcgg ttcacgccca
     2821 tttgcccgtc aatagtaaaa gcgtggcggg tacgggttgc gaggaaacac tcttcacggc
     2881 taagggaaac tttcaccgga atgccgccaa gcttgctggt caggaatgcc gccattggct
     2941 cttccagtac atcctgttta ttaccaaaac cgccaccgac aaacggtttg atgactcgta
     3001 cgcatgacca gggaatatcc agcgcctgac caaccacgcg gcgaacaatg tgcgggatct
     3061 gggtgctgga aacgatggta attcgcgagt catcctccat ccacgccagc gatgttacgc
     3121 tttccatgtg acaatgttga ataacgggag tctgatagtg cccctgtacc tggtagtcgg
     3181 cggcatcgat tgtttgttgg acattgcccg tcgacatcgt gctttgtttc agtaaattgc
     3241 cgccgttatg gattggtgca gcgtcttctg tcagcgccgc ttctggcgtg gtgataacgg
     3301 gtaattcttg ccactcaatg ctgaccaatt gcgccgcttt ttctgccgtg agttcatcgc
     3361 gggccacgac gatggcaacg gcgtcaccat gatgacgaac atggcgagtt agcagtgcgc
     3421 gatcggcggt atcgcgcttg ttttcgtcaa gtgtccaggc atgcccagct gtagcgaatg
     3481 gaatatcagg cacatcctcc caagtaaaaa tcgccagtac gcccggtaaa cttctggctt
     3541 gttcatcatt aatacttacg gcataaccat gtgcgatagg gctacgtaca tatttcgcgt
     3601 aacacatgcc cgccataaca taatcgtcag tatatcgtgc ccgcccggtg accttagcaa
     3661 tggcatcgac gcgcatgcat gattcacccg tagcggttgc ttcccgcgct tccatagaaa
     3721 tcccctcgat ttgaacattt ggttcttatg gcaaagattt agtctaagca ggcaagattt
     3781 acgccagaaa cgacgataag cgttagggga ggaaggaagt gtgatgacgt taaaaaaaca
     3841 cagatatgat acgaaacagg actctgaata gggacagtga tacgtatcaa tatgagtggc
     3901 gataaaatca atgtgataaa tcaggattat tacgcatcag gatgggaaat agggtaaaaa
     3961 gtcaggaact tcgatacctc tacagcgaat attttgctga tgatacttcc ctttgccaaa
     4021 tgaaattaac ctctttctct attaccacgt ttttccagaa gcaagagatt gggtatcaag
     4081 aggctggctg ctatgatatg gcgtcttgtt ttttaagcga ggaaagattt tgagtgcggg
     4141 acgcctgaat aaaaaatctc tgggtatcgt gatgttttta tcggttggac tgctattggc
     4201 gggctgttcg ggtagcaaat catccgatac aggaacgtat tccggctccg tttacaccgt
     4261 gaaacggggg gatacgctat atcgtatttc gcgcaccacg ggaaccagcg taaaagagct
     4321 ggcgcgactg aacggcattt ccccccctta caccattgaa gttggtcaga aactaaaact
     4381 gggtggggcg aaaagtagca gtagtacacg taaatcaacc gccaaatcaa cgactaaaac
     4441 cgcatcggtt acaccgtcat cagcggtacc gaaatcttcc tggccgccag tagggcaacg
     4501 ttgttggtta tggccaacga cagggaaagt tatcatgccg tattcgacag cagatggcgg
     4561 caataaaggg attgatatct cagctccacg gggtacacct atttacgccg cgggtgcagg
     4621 aaaggtggtg tatgtgggca accagctgcg tggctacggt aatctcatca tgattaaaca
     4681 cagtgaagat tacattacgg cttacgccca taatgacacg atgctggtaa ataatgggca
     4741 aagcgtgaag gctgggcaaa aaatcgccac tatggggagc acggatgcgg catctgttcg
     4801 cctgcatttc cagattcgtt accgtgcaac ggcaattgat ccgctacgtt acttgccgcc
     4861 gcagggcagc aagccaaaat gctgatggcg aattaatcag cagtcagcag cgcggcactt
     4921 gctaaggaga gcgtaaggtt tataatgcct tacgcatctc gaagcgggcg tagttcaatg
     4981 gtagaacgag agcttcccaa gctctatacg agggttcgat tcccttcgtc cgctccatga
     5041 cctttcttaa agtactccaa cctactgaat cctaagggat atggctttaa gcctccttgc
     5101 agagttactt actgtaaccc actgtgtaac cctctcaggg catacagaca tatgattcag
     5161 ctatccagta gtcacaaact tccagcagtc tactatctct accagcgtaa cggcgtctac
     5221 tacttccgtc ttcgggtcag acaatcaaac aatgatagaa tgacctcagt atcattacgg
     5281 accaaggata gacgcaccgc tatggctcac tcaaggcaca tcaaggcagc actcaaggca
     5341 atacacgcag acagacccaa cgctacctat gaggagatga gggagcacct taaggacatc
     5401 gctgagtgtg aactgtctat gggccgttca gatctctttg agcctgacat gagggatatc
     5461 taccgggacc aatacggaga acttggtgag agccttaccg atgctctggc tagtgagccg
     5521 ctaagtattg accagcatcg ttacatcaat gaggccctga aggtgctaaa agcctgtatg
     5581 aggcgtatcg aagcgggaga tagtcagcct ctgatagatt acgtcgatcg ttttaatgat
     5641 attgatcggc aagatgacca agcagactct gtgtccctat ctgttagtgc tccagaagta
     5701 aaaccagaag ttacgcctag cattaccata gcgagcctct ttgagcaata cgaagcggag
     5761 aactctcaga actggaagcc tgcaacctta agggaaaaca aagcgtcaca cgctgcgtta
     5821 atcgaaatat tcgaccatct gggtctcaat gcagacgcta acagggctga catgcttaag
     5881 gtccgtgatg tgctccagca gttacctaag aaccgtaagc agcgctttaa ggatgcccca
     5941 cttgctgacc tgttgagccg tgaggataag acagattgtc tggatgtcgt taccatcaac
     6001 aacaagtatc ttattaagat ggctgctgtc tttaggtggg ctgtgcgcaa tgacttgatt
     6061 aaaaagaaca tgactgaggg tctggagctt aaagtccctc agcgtaaagc ctcaggggcc
     6121 cgtaacgcct tctctacgga gcaggtgggg caactactgg tcgcagctaa ggcatactct
     6181 cagaagacct ctggtaagcc ctaccactat tacgttacgg ctttggctgc tatcactggg
     6241 gcgagactca atgaaatagc ccaactacag gtcaaagacg tcaggaccac tgaggcggga
     6301 accgtttata ttcatatcaa tgaagacgat agtagtctac ccggtaagag catcaagaac
     6361 gctcatagtg accgctgtgt gcctctggtc gatggggcct atggttttat attggctgac
     6421 tttatggcgc ttgtagagac ccgtagaggc gctgacggtg atgacgctat ggtgttcgat
     6481 ggtctgcgtc tcatgaagaa cggttacggt gagcaggtga gtaaatggtt taatcggacg
     6541 ttgttgccta aagtcctcgt agaccgtagc ggcttagcgt ttcactcgtt tagacataca
     6601 gttgcgaccc agttaaaaca acatggggtc gagttggcgt atgcacaggc gatcatgggg
     6661 catagttcag ggtctattac gtacgaccgt tatgctaaag aggtcgaggt tgataggctg
     6721 gtcaatgtga tggctgatgt ttataaggag acctagagga gaccatagtc taggatgaca
     6781 cttactacag acctgtgtgg tcatgttttt gactcatggt tttctctact tgtcaaggga
     6841 taattaatac gactcacta
//
LOCUS       DAEEGA010000151         6763 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEEGA010000151 DAEEGA010000000
VERSION     DAEEGA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.152"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            64..2496
                     /locus_tag="KLH46_003805"
     CDS             64..2496
                     /locus_tag="KLH46_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBE0380315.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2563..4032
                     /locus_tag="KLH46_003806"
     CDS             2563..4032
                     /locus_tag="KLH46_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBE0380316.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4032..5693
                     /locus_tag="KLH46_003807"
     CDS             4032..5693
                     /locus_tag="KLH46_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBE0380317.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5914..6255
                     /gene="symE"
                     /locus_tag="KLH46_003808"
     CDS             5914..6255
                     /gene="symE"
                     /locus_tag="KLH46_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HBE0380318.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6302..6622)
                     /locus_tag="KLH46_003809"
                     /pseudo
     CDS             complement(6302..6622)
                     /locus_tag="KLH46_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2001 a         1612 c         1556 g         1594 t
ORIGIN      
        1 tcatagccag gctattgatt ttatgtacaa tcagaatcgt cctgataaaa gcgagaaccc
       61 cccatggcgg aactgaacct aagtaacctg acggaagcag acatcattac caaatgcgtt
      121 atgccagcca ttctcaatgc aggctgggac aacacaacgc aaatcagaca ggaggtcaaa
      181 ctccgggacg gtaaagtcat cgtacgtggt aaagttgcgg cacgcagaac ggtaaaatct
      241 gctgacatcg tgctgtacca caagccaggc attcccttag ccgtgattga agcaaaagcc
      301 aacaaacatg aaattggcaa agggatgcaa cagggcattg aatatgcgcg cctgctggac
      361 gttccttttg tttttgccac caacggtgat ggctttatct tccgcgatgc caccgcagcc
      421 gaaggtgaat gccttgaaaa gcaaataacg ctggatgact tcccctcccc cgctgaactc
      481 tggcaaaaat tctgcctctg gaaaggatac acacaagctc agcttccggt gattactcag
      541 gactattacg acgatggcag cggcaaatcg cctcgttatt atcaacttca ggcaatcaac
      601 aaaaccattg aagccgtctc caacgggcaa aaccgcgttc tgctggtcat ggcgaccgga
      661 acagggaaaa cctataccgc attccagatc atctggcgcc tgtggaaatc aaaaaataaa
      721 aaacgcattt tgttccttgc cgatcgcaac attctggtcg accaaaccaa aaataatgat
      781 ttccagccat ttggtacggc aatgaccaaa gtcagcggac gcaccattga tcccgcttat
      841 gaaattcacc tcgcgctcta tcaggctata actggcccgg aggaagacca aaaagcgttt
      901 aaacaagtcg caccagattt cttcgatctg atcgtgatcg acgaatgcca tcgcggcagc
      961 gcatccgaag acagcgcctg gcgagagatc cttgattatt tcagttccgc cacccaaatt
     1021 ggcttaaccg ccacgccaaa agagacgcat gaagtctcca gcacggatta cttcggcgat
     1081 ccggtttacg tctactcact aaaagaaggg atcgaagacg gcttcctcgc tccttataaa
     1141 gttgtccgtg ttgatattga tgtcgacctg caaggctggc gcccaaccaa agggcaaact
     1201 gacttaaacg gcgaagtgat tgacgatcgt atctataacc agaaagattt cgatcgcacg
     1261 atggtgatcg acgaacgcac agaactggtt gccagaacca ttaccgacta cctcaagcgc
     1321 accaatccga tggataaaac catcgtcttc tgtaacgaca tcgatcatgc agaacgtatg
     1381 cgccgcgccc tggttaatct caacccgcag caggtgaaaa agaacgacaa atacgtcatg
     1441 aaaatcaccg gcgatgatga aattggcaaa gctcagttgg ataacttcat caacccgaaa
     1501 aaaccgtacc cggttatcgc gaccacttca gagctgatga ccactggtgt ggatgctaaa
     1561 acctgcaaac tggtagtact agaccagaac atccagtcga tgaccaaatt caagcaaatt
     1621 atcggtcgtg gtacacgcat cgacgaacgt tacggcaaac tctggtttac catcctcgac
     1681 tttaaaaaag ccaccgaact gtttgccgat gagcgtttcg atggcattcc cgaaaaagtc
     1741 atggatacca caccagagga tatcgccgat ccagaatctg attttgaaga gaaactcgaa
     1801 gaaatcagcg aacatgacga cgaacaggta acaggcgttg atgaaccgcc tgcgccacca
     1861 taccaggtta ccgataccga tgatgtcggc ccatttccgg aagaagacga gaagaaaatc
     1921 cgcaagtttc acgtcaacgg tgtagcggtg ggcgttattg cacagcgcgt tcagtattac
     1981 gacgccgacg gtaaactggt taccgaatcc tttaaagatt acacccgcaa aacactgctc
     2041 aaagaatatg cctcgctgga tgactttacc cgcaagtggc aggacgccga tcgcaaagaa
     2101 gcgatcattc acgagctgga gcaacagggg atcatctggg aagtactggc agaagaagtc
     2161 ggtaaagatc tcgacccgtt cgacatgctt tgccacgtag tgtatggtca gccgccgtta
     2221 acccgcaaag agcgcgccga gaacgtgcac aagcggaact acttcacaaa atactctgaa
     2281 gcagcgcaag ccgtgctcga taatctgctg gataaatacg ccgatgcggg cgtacaggag
     2341 atcgaaagta ttcaggtgct gaaacttaaa ccattcgaca gcatgggcac cttaccggag
     2401 attattaaaa ccggatttgg cgaccgtaac gggtataatc aggcgctcag cgagctggaa
     2461 aacgaaatct accaattacc gccccgctct gcttaacaac tctcttttgc gccttctgca
     2521 accatcatgt tgcagaaggc cttgtttttc atggaataca acatgtctat cagctcagta
     2581 atcaaatcat tacaagatat tatgcgcaaa gatgccggtg tggacggcga tgcgcagcgt
     2641 ctcggtcagc tctcctggct gctgtttttg aaaatcttcg atgcccagga agaggcgctg
     2701 gaactggagc aggataacta tcaatatccg atcccacagc gttatttgtg gcgcagttgg
     2761 gccgccaacg cgcagggcat taccggtgat tccctgctgg aattcgttaa tgatgatctg
     2821 tttccggcgt tgaaaaacct cactgcgcct atcgataaaa acccacgcgg ctacgtggtg
     2881 aagcaggcgt tcagcgatgc ctataactat atgaaaaacg gtacgctact gcgccaggtg
     2941 atcaacaagc tgaacgaaat tgactttacc agcgccagcg aacgccatct gtttggtgat
     3001 atttacgaac agatccttaa agatctgcaa tctgcgggca atgcgggcga attctatact
     3061 ccacgcgccg tcactcgctt tatggtggat cgcgttgatc cgaaactcgg cgaatccatt
     3121 atggacccgg cctgcggtac gggcggtttt cttgcctgcg cgttcgatca tgtaaagaac
     3181 aaatacgtga agagcgtcgc cgatcatcag acactgcaac aacagatcca cggtgttgag
     3241 aaaaaacagc ttccgcacct gctggcgacc accaatatgc tgctgcacgg cattgaagta
     3301 ccggtacaaa ttcgtcacga caacaccctg aataaaccgc tttcctcctg ggatgagcaa
     3361 ctggatgtca ttgttaccaa cccgccgttt ggtggcacgg aagaagacgg tattgagaag
     3421 aactttccgg cagagatgca aacccgcgaa acggcggatc tgtttctgca actgattgtg
     3481 gaagtactgg cgaaaaacgg tcgtgcggcg gtggtattgc cggatggcac actatttggc
     3541 gaaggtgtta aaaccaaaat caaaaagcta cttaccgaag agtgcaacct gcataccatc
     3601 gtgcgtttac cgaatggtgt gtttaatccc tataccggca ttaaaaccaa cctgttgttc
     3661 tttaccaaag gtcagccaac caaagagatt tggttctatg aacatccgta tccggcgggc
     3721 gtgaaaaact acagcaaaac caagccgatg aagtttgaag agtttcaggc ggagatcgac
     3781 tggtggggta acgaggccga tggttttgcc agccgcgtcg agaatgagca agcgtggaaa
     3841 gtcagcattg atgacgtgat tgcgcgcaac ttcaatctgg atattaaaaa cccacatcag
     3901 gcggaaaccg tcagccatga tccggacgaa ctgttagcgc agtatgcaaa acagcaggcg
     3961 gagatccaga cgctgcgtaa tcaactgcgc gatattcttg gcgctgcgct gtctggcaag
     4021 gaggttaact aatgagtgtg gaaaagctga tcgttgatca tatggaaacc tggacctcgg
     4081 cgttgcaaac ccgttccacc gccgggcgcg gcagctccgg taagattgat ttgtatggta
     4141 ttaagaaatt gcgtgagctg attctggaac tggcggtgcg cggtaaactg gtgccgcagg
     4201 atccgaatga tgaaccggcg tcggacctgc tgaagcgtat tgcggcggaa aaagcagagc
     4261 tggtgaagca aggcaaaatt aaaaagcaaa aaccactgcc agaaattagt gaggaagaga
     4321 agccgtttga attgccggag ggatgggagt gggtgcacct ccctgatatt tattgctcaa
     4381 tttctgagtc atctagaaaa ataaaatcct ccgaaatact acctgaggga aaatatcctg
     4441 ttatcgagca aagccaagaa tttatttctg gttattgcaa caatgaatgt ctgttaataa
     4501 agttaaataa tccagttata gtttttggtg atcacacaag gaatattaaa tttattgatt
     4561 ttgattttgt tgtcggcgct gacggtgtga agattttatc acctatatta atttgtgagc
     4621 gtttcttttt ttggcagtta cgatcattta aattagatgt aagaggatac gcgagacatt
     4681 ttaaagtatt aaatagctgc ttatttgcat taccccccat tgcagaacaa gagcgaatag
     4741 ttgaaaaagt tagctctttg atgtccctct gcgaccaact agaacagcaa tccctgacca
     4801 gtctggacgc acatcagcaa ctggttgaaa ccctgttggg aacgcttaca gacagccaaa
     4861 acaccgcgga actggctgaa aactgggcgc gtattagcga gcatttcgac accctattta
     4921 ccaccgaagc cagcgtggat gcgttaaaac agaccattct gcaactggcc gtaatgggca
     4981 aacttgtgcc gcaggatccg aatgacgaac ccgcctctga actgctcaaa cgtattgcgc
     5041 aggaaaaagc tcaattggtg aaagaaggga aaataaaaaa acaaaaacca ctgccgccaa
     5101 ttagcgatga ggaaaaaccg tttgaacttc cggaagggtg ggaatggtgt cgtattggta
     5161 atatagttaa tattaaatct gaattagtat caccaaaaga ttatttaaat ttatatcaag
     5221 tagcacctga tattattgaa aagggcactg ggagagtgat atcaaagaga acagtgaaag
     5281 aatcaggagt caaagggcca aatagtcgct tttataaagg acaaattgtt tattctaaaa
     5341 tccgcccgtc attatccaaa gtttttttag cagagtataa tggtctttgc agtgctgata
     5401 tgtatccatt agactgttac attaatccaa attacttatt gaaatatatc ctttccattc
     5461 catttcttat gcaagtcaaa aaagcagaga acagaataaa aatgccaaaa cttaattctg
     5521 acagttttta caatattatt gttgcgattc ccccctataa tgaacaacaa gcaatatttg
     5581 ataagataaa ttctattgaa gcagtatgca atggtcttat ttcttatatt gggatttatc
     5641 ataaaaccca actgcatctt gcagacgcac tcactgacgc agcgataaac taattccctc
     5701 gccctcacaa aaccaataaa gcggctaaat agccgcttta ttcacatcac caaaatttat
     5761 atttcacgct aattttcatc ttattttcat taatttttag ctggactttc cctgtattta
     5821 ctgatgatgt atccaggtat ttagcacggt gcggatgtgc gccaacacac tcgcaccgct
     5881 aatcacaatc actatttata gagaatagta atcatgactg acacgcattc tattgcacaa
     5941 ccgttagaag cagaagtctc cccggcaaat aatcgtcaat taaccgtgag ttatgcgagt
     6001 cgttacccgg attacagccg tattcccgcc atcaccctga aaggtcagtg gctggaagcc
     6061 gccggttttg ccaccggcac ggcggtagat gtcaaagtga tggaaggctg tattgtcctc
     6121 accgcccaac ccgccgccgc cgaagagagc gaactgatgc agtcactacg caaggtgtgt
     6181 aagctgtcgg cgcgtaaaca aaggcaggta caggaattta ttggggtaat tacaggtaaa
     6241 cagaaagtcg cctgatacat tgacccggta gcgccagtgc taccgggtat tattcgccgc
     6301 gttataccaa cgcgctctcc atctgttttt caaatgcctg acgaattaat cccataatgt
     6361 aaggaagttg tgcgaggtcg ctgaaaccaa tttctacatc gccattaccc cagcgcccaa
     6421 catttgtcac atctttggca atacctttcg gatcgaccag ttcatgaaac tgcatattaa
     6481 gcgacaatcg cagtcggctt ttttgcggca caacatcaac aaaattcgtt tccgctttaa
     6541 acgcaatata tagcttcaat acttcctgcg tgatccctgc gtccaggcgc ataacttcat
     6601 cgcgaagatg atcgaataac actgaggtag cctgagttta acggacactc cttcctgaaa
     6661 tagaatggca tcagaaggag ctaataatga gcagaaaaac ccaacgttac tctaaagagt
     6721 tcaaagccga agctgtcaga acggttcttg aaaatcaact ttc
//
LOCUS       DAEEGA010000152         6709 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEEGA010000152 DAEEGA010000000
VERSION     DAEEGA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.153"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..243)
                     /locus_tag="KLH46_003810"
     CDS             complement(<1..243)
                     /locus_tag="KLH46_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0380319.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEICRLQKELKRVTDERDILK"
     gene            complement(320..1534)
                     /gene="rspA"
                     /locus_tag="KLH46_003811"
     CDS             complement(320..1534)
                     /gene="rspA"
                     /locus_tag="KLH46_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HBE0380320.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1740..2066)
                     /locus_tag="KLH46_003812"
     CDS             complement(1740..2066)
                     /locus_tag="KLH46_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HBE0380321.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            2201..2542
                     /locus_tag="KLH46_003813"
     CDS             2201..2542
                     /locus_tag="KLH46_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBE0380322.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2608..3137
                     /gene="speG"
                     /locus_tag="KLH46_003814"
                     /pseudo
     CDS             2608..3137
                     /gene="speG"
                     /locus_tag="KLH46_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(3140..3850)
                     /locus_tag="KLH46_003815"
     CDS             complement(3140..3850)
                     /locus_tag="KLH46_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBE0380323.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3958..4263
                     /locus_tag="KLH46_003816"
     CDS             3958..4263
                     /locus_tag="KLH46_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBE0380324.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4462..4785
                     /locus_tag="KLH46_003817"
                     /pseudo
     CDS             4462..4785
                     /locus_tag="KLH46_003817"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5341..5919
                     /locus_tag="KLH46_003818"
                     /pseudo
     CDS             5341..5919
                     /locus_tag="KLH46_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1776 a         1702 c         1571 g         1660 t
ORIGIN      
        1 ttttaatatg tcccgttcgt cggtaacccg tttcagctct ttctggagac ggcagatctc
       61 ggcctgagca tctgactgtt ctttattagt ggaagaatcc ggaccgtact tctttatcca
      121 ggcataaagg ctgtgggtgg tgatatcgag acgtgttgca acgctggcaa cagaataacc
      181 gcgatcaaca acctgtttga ctgcttcagt tttaaactct tcgggataac gcttaccgct
      241 catgggcacc tctctttaag ccatcttaaa tgactctgag gtgtctgtta aacccgtggc
      301 gattcaatta tcttactcct tacctgttcc acagcgtgcc atcttccaga cgtgcgactg
      361 gtagataagc aggttcatag ggatatttcg ccgccagctt ttcatcgaat tcgataccaa
      421 gacccggttt gtctcccgga tgcatatagc cgttatcgaa agtccagttg tgcgggaaga
      481 cttcgagcat ttgttcggaa taacccatgt attcctggac accgaaattg gggacccaca
      541 gatcaaagtg cagcgccgca gccatgcaga ctggtgacaa atcggaagga ccgtgtgagc
      601 cagtacgtac ctgatacagc gaagcaaaat cggcaatccg gcgcataccg gtaattccgc
      661 ctgcatgggt cagcgtggtg cggatataat cgatgagttg ctcttcaatc agttgtttgc
      721 agtcccagat gctgttgaag acttcaccca ctgcgatggg tgtgacggta tgttggcgaa
      781 tgagacggaa gcattcttgg ttttccgctg gcgtcgggtc ttccatccag aacatgcgat
      841 aatcttcaat gcttttacca aagcgcgccg cttcaatagg cgttaagcga tggtgcatgt
      901 catgcagcaa atgttcatta aaaccaaact tgttacgtac cgcgtcaaac aatttcggca
      961 tgaaatcgag gtatttctcc gtcgaccaca gctgctcttc cggccactgt cctttggttg
     1021 cgggttcata agccagacct ttacctttcg acatgccgta ggtggttttc ataccaggga
     1081 ttccgcactg cacgcggatg gctttgaatc ccagctcctg atgacgggca taatcatcca
     1141 gagcttcatc aatactgtga ccggtggtat ggcaataaac catcacccct tcacgagacg
     1201 cgccgccgag taactggtaa agcggcatgt tggcagcttt ggctttaata tcccacagcg
     1261 ccatatcaac cgctgaaatg gccgacatcg taaccggacc gcgacgccag taagcacctt
     1321 tatagaaaaa ctgccagata tcttcgatac ggtgcgcatc gcgaccaata agccgcggac
     1381 aaagatgatc ctgcaaataa gaggccacgg aaagctcacg tccattgagg gtggcatccc
     1441 caaggcccgt aataccgtcc tcagtggtga tttttaatgt gacgaaatta cgccccggac
     1501 aggtaacaaa aacttcagcc tttacgatct tcatgttcga ttccttgcat cgcttgtcgt
     1561 gatgcatgaa atctacgcaa ctgagctact gccatacaag tatgaagatc gaaaaaagcc
     1621 ggagtgatca caaaaaaagg cgtatatttg cgctgtgaat ggttgacaaa agatgaaata
     1681 gaataccttt tgtcagctga cacttcctcg tatcttattg ataaaatgga tttatgttcc
     1741 tacgtgcgcc cccagcccgc aacaatgatc aacattccgc aaagcgcaat caacgcaccc
     1801 gtccagtcat aaagacttag tttcacgcca tccacaacgc gcagccacat caatgccgtg
     1861 cagacataaa cgccaccata agccgcgtaa acacgcccac tcgccgcagg atgcaacgtt
     1921 aacaaccaga caaacagcgc cagtgaaatc cccgccggaa gcaacagcca gatactggcg
     1981 tttcgtttta accacaacca gggcagaaag catccaataa tttcacacag cgcagtagca
     2041 aaaaatagta acgttgtttt aatcatcttt gtcacttatt gacatcatgt atagttatag
     2101 ggcgacataa tatcatcaat ataaacatcc tcctggtacg ttttgcgttc gcagtggatg
     2161 gtgtagaatc acctttaatc attcatacag ggaatgaatt atgaaaatca ctctcagcaa
     2221 acgaatcggc ctgctcgctt ttctgctgcc ttgcgcactg gcattgagca caactgttca
     2281 tgccgaaact aacaaactgg tgattgagtc tggcgacagt gcacaaagcc gccagcacgc
     2341 cgctatggaa aaagagcaat ggaatgacac gcgcaatctg cgccagaaag tgaataaacg
     2401 cactgaaaaa gagtgggata aagccgacgc cgcttttgat aaccgcgata aatgtgagca
     2461 aagcgccaac atcaatgcct actgggagcc caatactttg cgctgcctgg accgtcgaac
     2521 tggccgcgtt attaccccct aacctgttat tgatttaagg aatgtaagga cacgttatgc
     2581 caagcgccca cagttaagct acgcccgctg gagcgtgaag atttacgcta tgtacatcaa
     2641 ctcgacaata acgccagtgt gatgcgttac tggtttgagg aaccctacga agcctttgtt
     2701 gaactctctg atctgtatga taagcgcata ttcacgatca gagcgaacgg cgctttgtgg
     2761 tggaatgtga cggcgaaaaa gccggtctgg tggagctggt ggaaattaac catgttcatc
     2821 gccgggcaga atttcagata attatctccc cggagtatca ggggaaaggt ctggcaaccc
     2881 gtgccgccaa attagcaatg gactatggct tcaccgttct caatctctat aagctgtatc
     2941 tgatcgtcga taaagagaat gaaaaagcga ttcacattta ccgcaagctt ggctttacgg
     3001 ttgaaggtga attgatgcat gagtttttta ttaatggtca atatcgtaat gccattcgca
     3061 tgtgtatatt ccagcatcag tatctggcag agcacaaaac accgggtcag actctcctga
     3121 agccgaccgc acaatagcat taataataat cgatcgtatt tttgatggtg taaacccgtt
     3181 cgacggcggg ttttactcct tcatcaacaa tgatcagctg acagtccact gggttggcgt
     3241 gactgtcata ttcacagctc tgttttacat taccaactcg ttgattattt agtaaagtaa
     3301 ccgctgtgta atctaatttt ttgatcggat ccgttgatgg cgtggcgctg acagataatg
     3361 ttttgtcgtt acttttcgtg gttttaccca gcggataccc ctgatcatca tagcgatatt
     3421 ccatctgcat ttgtttgctg ctggctttaa tcacgaagcc attatcatcg gtttcccaac
     3481 tcaccccggc agaaggtaat tctgctagct ggcattttcc ctgtaaacgt actctcttct
     3541 ccagcgtctc ggcatcacgg taataattgg cgtccagtac cagagcgacc acggtattat
     3601 tttccagatc cagtaattcg agtgaatcaa aacagccttc ttccgacaaa gtcccagaaa
     3661 cacgtttcgt cacttcacct tgctcatcca ttaatgtctg agtgaaatct tttaccggac
     3721 cacgcagcgg atcaaaatcg aattcattag agaaactggc catctcaggg gtaaatgaaa
     3781 gtgttacctc tgtgcggtca catcctgtga ggaatatcgc gagtaagcat ggtagtaatt
     3841 tgtatttcac aacagtcacc agagagtaga gatgattctc aatcatagta gcaaatacag
     3901 tactttacac gttaaatgct atgcttaaag aagttatctt cgcgtaagga gcttatgatg
     3961 aaactctcaa cctgctgtgc cgcgcttctg ctcgccctcg cctcacccgc ggtactcgcc
     4021 gcgccgggat cttgtgagcg catacagagc gatatatcac agcgcattat caataatggc
     4081 gtacccgaaa gcagcttcac gttaagtatt gtacccaatg accaggttga tcagcctgat
     4141 tcccaggtcg tcgggcattg cgctaatgat acgcataaaa ttctctacaa ccgcaccacc
     4201 agcggtaacg tctctgctcc cgcgcagtct acccaggatg gcgcgcctgc cgaaccgcag
     4261 taacacatta tcgactgaac gccggatatg acacatccgg cgatttgaac atacaacata
     4321 atctcacctt attactcata cccttctatt gatgtggatt aataattctt aacccaaaat
     4381 gggtagactc cctctattgt tagcgcgcta aatattcaat atataaacat ttatataaca
     4441 ataaagaaca gggagtgagt tatgtccaaa aatgaccgaa tggtgggcat cagccgcaga
     4501 acactcgtta aatcaaccgc gataggttct ctggcgctgg ctgcaggcgg tttttcttta
     4561 ccgttcaccc tgcgcagtgc agcggcagcg gtacaacagg ccagcgaaaa agtgatctgg
     4621 ggttcctgtt ccgtcaactg tggtagccgc tgtgcacttc gtctacatgt taaagataat
     4681 gaagtgacct gggtggaaac tgacaatacc ggcagcgatg agtacggcaa ccatcaggta
     4741 cgcgcctgtt tgcgcggtcg ctccatccgc cggcgtatta atcatcccga tcgcttgaat
     4801 gacccaatga aacgcgtggg taaacgcggt gaaggcaaat tcgaacggat tagctgggat
     4861 gaagccctgg atactatcgc cagtagcctg aaaaaaaccg tcgaacaata tggcaatgaa
     4921 gctgtatata ttcagtactc ttcggggatt gttggcggca atatgacccg ctcttcgcca
     4981 tcagcctcgg cggtcaaacg cctgatgaac tgctacggcg gttcactcaa tcagtatggc
     5041 tcctacagca ccgcgcaaat ttcctgtgcc atgccctaca cctacggcag taatgatggc
     5101 aacagcacca cggatattga aaacagcaag ctggtcgtga tgtttggtaa caacccggca
     5161 gaaacccgca tgagcggtgg tggcatcact tatcttcttg aaaaagcgcg cgagaaatcg
     5221 aacgccaaaa tgattgttat cgatccgcga tataccgata cggctgccgg tcgtgaagag
     5281 gaatggctcc ctattcgccc gggcaccgat gccgcgctgg ttgcgggtat tgcctgggta
     5341 ttgattaacg aaaatctcgt tgatcaacct tttctcgata aatactgcgt cggctatgac
     5401 gaaaaaacct tacccgcaga tgcacccaaa aatggtcact ataaagccta tattcttggt
     5461 gaaggtgacg ataaaacagc aaaaacgccg cagtgggctt cgcaaattac cggtatcccg
     5521 gtagaccgta tcatcaaact ggcgcgtgaa attggcacaa caaaacccgc ttatatctgc
     5581 tagggctggg ggccgcaacg ccaggcaaac ggcgagctga ctgcacgcgc tattgctatg
     5641 ttacctattt tgacgggcaa tgtcggcatc agcggcggaa atagtggcgc gcgtgaatcg
     5701 acctatacca ttacgataga acgcctaccg gtgctggata atccggtcaa aacgtcaatc
     5761 tcctgcttca gctggacgga tgctatcgat catggcccgc aaatgacggc actccgcgac
     5821 ggtgtccggg gcaaagataa actggatgtg cccattaagt tcatctggaa cttcaaggac
     5881 ttgaagcgga cccttagtag gcctgaggtc ctgcattgag tcaagacctt gtgtcattct
     5941 ttgtgccaga cttgtgtcat tagacgggat gtctagacgg ctggtcagcc cccccggtgg
     6001 cctcggtctg gacttgaggt gggcctatgg ggggaagtct gctggcttaa tgtattagat
     6061 ggtcattcac atttttctgt cagaaaccat cggtcccact gtgagtccaa acctaaggac
     6121 ctagaagtcc acccacagcc ttagacagag tgtcgaacac atccacaccg agtcgaccag
     6181 agaggaccat gtagagaccc acagagagga acacgagttt atagaccaga gtcaggaggg
     6241 acttcaggtc tgcggaagtc atagacataa agcccctatc tgttatgtta tattattata
     6301 ttaacaacag gtccagacca ttggtccaga ccatagttag ttaatcacag tgatagccca
     6361 ccacctgaag tcctgctgat attcagtcca gacttacctc tgctcatggt cttacctccc
     6421 tcggtctcct gaccttgggt atcgactaca gcttgctgag cgtcatctgg aggagctaca
     6481 ggaggcggga ccgaagcccc accttgagtc ccaccaccac ctgtcaggcc cttggcgatg
     6541 gacctgaagc ctttactaat gctcttaccc attggtctta cgcttcggtc ttggtgaact
     6601 tcaggcgttt gactacaggc aggacggcag tagctgagcc agtgcccatc ttgacggtcg
     6661 tagcagcttc agcgacaacg aacactttac cgaggatttc caagtcgtg
//
LOCUS       DAEEGA010000153         6661 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEEGA010000153 DAEEGA010000000
VERSION     DAEEGA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.154"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..236)
                     /locus_tag="KLH46_003819"
     CDS             complement(<1..236)
                     /locus_tag="KLH46_003819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380325.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEG"
     gene            complement(322..1203)
                     /gene="narH"
                     /locus_tag="KLH46_003820"
                     /pseudo
     CDS             complement(322..1203)
                     /gene="narH"
                     /locus_tag="KLH46_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
     gene            complement(1200..4940)
                     /gene="narZ"
                     /locus_tag="KLH46_003821"
                     /pseudo
     CDS             complement(1200..4940)
                     /gene="narZ"
                     /locus_tag="KLH46_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            complement(5022..6410)
                     /gene="narU"
                     /locus_tag="KLH46_003822"
     CDS             complement(5022..6410)
                     /gene="narU"
                     /locus_tag="KLH46_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HBE0380326.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
BASE COUNT         1586 a         1746 c         1756 g         1573 t
ORIGIN      
        1 ccttcgtcat caacacgtac aaaccagaag accagctttt tgtttcccgc atccacaaag
       61 aacggaatat tcaggtctgc gcagcattca acggcatcgt caaaactatc aaagcgcaga
      121 acttctgcgt cttcttcgtc aaaaaaatca tcttcgtgaa gcttcacgac atagcgggga
      181 agtttgcttc tttgagaggc gggtttacgt ttacggggtt tagctgaacg ggccatataa
      241 ccacctgaaa gacaatgaca ttgcctgttt ttataacggt aattgcagac catgacaagc
      301 cgcagccgtc aggctgccta ctcgatagag gtcaacttcg cgctcggtgc tcgacgcttc
      361 ctcgatgcgg tcggcgtcgt aaagcagcac gcccagatac cggatgcgac ccacgcaggt
      421 ttctgagcac acggtgggtt ggcctgactc aattcgcgga taacagaaga tacatttttc
      481 tgacttgccg cttttccagt tgaagtagat ttttttgtac ggacaaccgc taatgcacaa
      541 acgccagcca cggcatttat cctgatcaat cagcacaatg ccgtcttctt cgcgtttgta
      601 gatagcgccg cttgggcagg tcgccacgca gctgggattg aggcagtgtt cgcacaggcg
      661 cggcaggtac atcatgaagg tgttttcaaa ctgcccgtac atctcctttt gcatggcctc
      721 gaagttgcgg tcgcgggcac gtttttcaaa ctcgccgccc agcagttctt cccagtttgg
      781 gccccagatc actttgtcca tccgtttgcc gtcaatcagt gaacgcgggc gagcagtagg
      841 gatatgtttg ccttccggtg tgctatgcaa atgttcgtag tcgaaggtga aaggttcgta
      901 gtaatcatca atctgctgcg gcaccaccgg gttggcgaag attttggtta ttacgcccat
      961 cttgctgccc agacgcggac ggatcttgcc attcacatcg cggacccagc cgccttgcca
     1021 ctcttcctga tcttcccagt ttttcggata accaatgccc ggtttggttt cgacgttgtt
     1081 aaaccatgcg tactccatgc cttcgcgtcc ggtccagacg ttgttacagg tcaccgaaca
     1141 ggtatggcag ccgatacatt tatcgaggtt aagcaccatg ccgacttgcg aacgtatttt
     1201 cattttttcg cctcctgtac ctgatcgcga ccttcatcat ccagccagtt aacgttcttc
     1261 atcttgcgga tcatgatgaa ttcgtcgcgg ttcgagccga cggtgccgta gtagttaaag
     1321 ccccaggcca gctgcgcgta accgccaatc atatgcgtcg gtttcgggca aacgcgggtg
     1381 accgagttat gaatgccgcc gcgcatgcca gttacttccg aaccaggaat attcataatg
     1441 cgttcctggg cgtgatacat catggtcatg cccggcggta cacgttggct gaccaccgcg
     1501 cgggcagtca gcgcgccgtt ggcgttaaat acttccaccc agtcgttatc gacaatggtc
     1561 agttctcggg catctgtttc gctgatccag acaatcggtc caccgcgaga gagcgtcagc
     1621 attagcaggt tttcactgta ggttgagtga atgccccatt tctggtgcgg cgtcaggaag
     1681 ttaagtgctt tttcctggaa gccgtttggc ggtatctggc gcatctcact gacgctacgg
     1741 gtgtcgatag gcgggcgata agccaccagg ctttcaccaa aagcacgcat ccacggatga
     1801 tcctgataga gctgctggcg tcccgacagc gtgcgccacg gaattaactc atgaacgttg
     1861 gtgtatcccg cgttatagga tacgtgatcg ctttccagac cggaccatgt ggggctggag
     1921 ataattttac gcggctgcgc ctgaatatcg cgaaagcgaa tcttctcgtc ctctttgtgc
     1981 agcgccagat gggtatgttc gcgcccggtg atctcgccca gcgcctgcca cgctttgact
     2041 gcaacatgac cgttggtttc cggtgccagt gccagaatca cttctgaagc gtcaatggcg
     2101 gtgtcaatca gcggacgccc ctgtgccggg ccatcacgct tggtgtaatt gagtttgccg
     2161 aggaaatcga tttcatcctg ggtattccac gaaatccctt taccgccatt gccaagtttg
     2221 tccatcaatg gcccgagcga ggtaaagcgt tcatacgtag cagggtagtc gcgctccacc
     2281 gccacaatat tcggcgcggt tttacccggg atcagatcgc attcgccttt gcgccagtcg
     2341 agcacttcac acggctgtga gagctctgcc ggagagtcat gcagcagtgg ttgtaatacc
     2401 acgtcggttt ctttgccaag atggcccacg cacacttgcg aaaatgcttt ggcaatacct
     2461 ttgtagattt cccagtcgct gcgtgattcc caggccggat cgaccgccgc agaaagcgga
     2521 tgaataaatg gatgcatatc cgaggtgttc atatcgtctt tttcatacca ggtggcggtg
     2581 ggcagaacga tatcggagaa caggcaggta ctggacatgc ggaagtcgag cgtcaccagc
     2641 aggtcgagct tgccttcaat cgccgcagtt tgccactcga cttcttccgg tttgatcccg
     2701 tcgctggcac cgagttcctc gccctgaatc ccgctttcgg tccccagcag atacttctgc
     2761 atatactcgt ggcctttgcc ggaggagcca agcaggttag agcgccagac aaacaaatta
     2821 cgcggatgat tgctgccgct atctggttgt tcgcaggcca tacgtaaatc gcccgatttc
     2881 agcgcctggg cggtaaattc tgtgggggat aatccggcct tgtcggcttc agctttaatc
     2941 ccgagcgggt tacgccccag ctgcggcgca gaaggtagcc agcccatacg ttcggcgcga
     3001 acgttgaaat caatcagatg accgctgtac ttactggcat cggcgagcgg tgaaagtaac
     3061 tcctgagcag agactttttc atagcgccat tggctggaat gattgtagaa aaacgaggtg
     3121 ctgttcatct ggcgcggtgg tcggttccag tcgagcgcaa aggccagtgg caaccagccg
     3181 gtttgtgggc gcagtttttc ctggccgaca tagtgtgccc agccgccacc gctttgcccg
     3241 acacaaccgc agaagatcag catattgatc atcccgcggt agttcatgtc catgtgatac
     3301 cagtggttaa cacctgcgcc gaggataatc attgagcgcc catgcgtttt atgggcggta
     3361 tcggcaaatt cgcgggcgat ggtttcaata tactggcgcg gtacgccggt aatttgctca
     3421 ccccaggctg gggtgtacgg tttgatttca gcgtaatctt tcgcgctgtt ttcatcttcc
     3481 agcccgcgat cgaggccgta attcgccagt accaaatcat aaacgctgac caccggacag
     3541 gtgttgccat cgactaacgt caggttttta acgggcaatt ggcgcaccag caccggattg
     3601 tgttttacgc tgcgaaaatg cggattttca atgccgccaa agtaggggaa ggccacgcct
     3661 gcaacagcgt catgttgacc gagcagggtt aacgacaatt cggtttccgt accggcggca
     3721 atggattcca gattccattt gcctttttct ccccagcgga aaccaatcga accgttcggc
     3781 actaccaatt cacctgcggt attaactgct acggttttcc actgcggatt gttgctttcg
     3841 cccagtccat ccaccaggtc agatgcgcgg atcatgcgcc cgggaacgta gctaccatcg
     3901 tcgcgaggct ccagcattac cagcatcggc atgtcgctgt agcggcggca gtagttgata
     3961 aagtagtcgc tgggattatc gagatgaaac tcttttaaaa tcacatgccc cattgccatc
     4021 gccagggcgc tatcagtgcc ttgtttcggt gccagccact ggtcgcacaa tttggccact
     4081 tcagagtagt caggggtaat ggcgatggtt ttagtgcctt tgtagcgtac ttcggtaaag
     4141 aagtgggcgt ccggcgtacg tgtctgcggt acgttagacc cccaagcgat gatgtagctg
     4201 gagttatacc agtcggcaga ttccggtacg tcggtttgct cgccccaggt catcggcgag
     4261 gcgggcggca ggtcgcaata ccagtcgtag aaactttaac aggtgccgcc aagcagcgac
     4321 agataacgcg ttccggcggc gtaagaaacc atcgacatcg ccgggatcgg cgagaaaccg
     4381 gcaacgcgat ccgggccgta ggttttgatg gtccagacgt tagcggcggc aatcagctgg
     4441 tttagttcct gccagttgga gcggataaac ccgccgcgcc cacgcacttg tttgtagctc
     4501 aggcactttt gcggatcgtt cataatcgat gcccacgcca gtaccggatc gctgtgttgt
     4561 ttgagggctt cgcgccacag ttcaatcagt cgtttacgaa tgagcgggta tttcaggcgg
     4621 ttagcgctgt aaagatacca ggagtaactt gcgccacgcg ggcagccgcg aggttcatga
     4681 ttgggcaggt cagggcgagt gcgcgggtag tcggtctgtt ggatttccca ggtcaccaga
     4741 ccatttttaa cgtagatttt ccagctacag gagcctgtac agttaacgcc gtgggtggaa
     4801 cgcacgattt tgtcgaactg ccaacgctgg cgatagctgt cctcccagtc gcggttgcta
     4861 tgcatcacct gtccgtgacc atcggcaaag gtttcgccct tttgtttgaa gtagcgaaag
     4921 cgatccaaaa gtttactcat gacatgactc ctgctccagg aatgaattgg gatctgatgc
     4981 cttgtcggat gcgacatggt tgtacatcag gcatcgatct cttatttttg gctgaacttc
     5041 cgccgaccat aaaccagcca ggtcagcagc acacaaacga tgtagaagat taaaaacact
     5101 ttcatcgcgc cgaccggaga gccggtcata ttgagcgaca tgccaaatgc ctgcggaata
     5161 aaaaagccgc ccactgcgcc aatggctgag ataaagccca gcgttgccgc cgtttcggtg
     5221 acggcttctc tctgagcttg ctcatcacta ccgcctttca tcttcacccg ataaatggtt
     5281 atctggcgaa agatgacggc gatcatctgg aaagtagaac cacttcccag acccgcggtc
     5341 agaaacagcc ccataaatac ggcgtaaaag gcgatgaaat taccggagcc tgtgcccggt
     5401 aaggtaagga acagcagggc actgaaaatc gccataaaaa taaagttgat caacgtcacc
     5461 cgcacgccgc cgaatttatc ggaaatagca ccaccaaccg accgcgcgat ggcaccaata
     5521 aatgggccaa agaacgccag gcgcagaata ttcacatccg ggaactgggt ttttgccagc
     5581 atggcaaaac ccgcagaaaa accgataaac gaaccgaagg tggcaaggta aagcaggctc
     5641 agcagccaga gatgcaggcg ttgtaagaca gggagctggt cggcaattga ggcgcgtgaa
     5701 ctggcgatat cattcatccc tgaccaggcg gcgatcgtgg caatcgccag tagcggtacc
     5761 caaatccatg cggcattcgc cagcgacatc accgaaccgt cggcctgcgg tacgccattg
     5821 acgccgagaa aggcaaatac aggtacaaaa atgaccagcg gtgcaaccag ctgcattaca
     5881 cttacaccta agtttcctaa tccgccatta atcccaagag cgctcccttg tttggctttt
     5941 ggaaagaaga aactgatatt gcccatgctc gaagcaaagt ttgcacctgc aaaaccgcat
     6001 agcaaagcga taacgataaa tatcccaaaa ggagtattcg gattttgcac ggcaattccg
     6061 agccagacgc aaggaataat caggattgca gtactaaaaa ccggccatcg gcgtccaccg
     6121 aatataggca ccataaagga gtagggaaca cgcaataatg cgccagaaac ggagggtaat
     6181 gcggttaata aaaagagttg atcggtagta aaattaaaac cgattttatt gagattaacg
     6241 gtaactgcgc taaatagcat ccagacacag aaggcaagaa gtagacaact gactgatatc
     6301 cagaggtttc ttcgagcaat atgttttcct ttattttccc agaaggccgg attttctggt
     6361 ttccagtcgc gtaaaagata acgactattt ttctcatttt gcagtgccat attgttcctc
     6421 atatgcacac attggtaatg aaaaaagaca aaacaggagg taaggcgcaa tagccagtta
     6481 ttagaattaa ggatgaattg ggtgaagtgc tgattgaaag aatagataag aaagcgtaac
     6541 cgcgggggca gaatggggat taagtagcca gatatgtgtt actaaatgta actaacaagc
     6601 taaccttcaa acgggggtta atctttgaac gtatgtcatg tttcaaccct tcagatcgtg
     6661 a
//
LOCUS       DAEEGA010000154         6616 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEEGA010000154 DAEEGA010000000
VERSION     DAEEGA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.155"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(97..2100)
                     /locus_tag="KLH46_003823"
     CDS             complement(97..2100)
                     /locus_tag="KLH46_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380327.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLSSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2097..3233)
                     /locus_tag="KLH46_003824"
     CDS             complement(2097..3233)
                     /locus_tag="KLH46_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBE0380328.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3226..5505)
                     /locus_tag="KLH46_003825"
     CDS             complement(3226..5505)
                     /locus_tag="KLH46_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380329.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFENESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5516..6604)
                     /locus_tag="KLH46_003826"
     CDS             complement(5516..6604)
                     /locus_tag="KLH46_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBE0380330.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1511 a         1822 c         1777 g         1506 t
ORIGIN      
        1 actggtgact ctcaccaggg gctatatagg atatgcctaa taccgtggcg tgagcagtcc
       61 ggaactggag tagaactctt agtaaaaagc actatttcat ccttgttgct gaagcatggg
      121 gaataattgt tcgcaaagca aaacaccgtt attcattgct tctacccgtg cctcgctttc
      181 tgtattacga aattgtgcca acacatgtgc cagccgataa aaacccaccg cggtgaggtc
      241 attcgccagc aactctgcct gattaatagc gctctgttcc tgatagcgcc agccgttatg
      301 gagcagttga ataagtaacg cctggcagcg catcagcaac tgatgagcag tagacggcac
      361 aggcagaacg ctggcagaag atagtgatac caccacaggc gcagtttctg cgtccagcgc
      421 ccaggcacga gtctttgtca tcattacctg tggttccagt gtcaattgcc catcaacaaa
      481 actgacaaag ccagaaacca gacacacggg gtcgtctgtt tgttgcaaaa gcgccgccat
      541 gcgttcaatg gcataaggcg cgctggctga ggctggcagt gataacgtca gcagattatc
      601 ttcaccttcg ccgctgatta cctgcgcatc cagcgtctgg cggctgctat cccaaccgag
      661 cgaaatacac tcagcgaccg gcagaataaa taagttatcg acctgattaa gaggccgtat
      721 gcaggcgggg ggacgctggc gtaaatattc ccgcaaagcc acaatgcccg gctggcgtaa
      781 cggcgcgctc aacatttgcc aggcatcagg cgacagcggt acaacgctgc ttaagcggtt
      841 gcgggtagct aacagcagct cgccatcggc actgcgtttt gctgcttgtg aaacaatttg
      901 cccacccgcc agtgcgccag cctgaaaact aaacagccga cgcgtagctg ccggtgagtc
      961 ttcctgttca cttcgcggcc aactgcgcga aaggtgcaaa atactgccgg tgtcgggatc
     1021 ggtaaaccag atgcgtaaac cataatgctc aatatcctgc cagcaacgca tacctaaaga
     1081 caccagccgc agatgatcaa gctttgcttc tccggcaatg ccagagccaa cgaccgtgcg
     1141 ccacggcaca ggaggaactt caccaacact gtcgctccgg gccatctctt gtgcgcaatt
     1201 taatcgactg tttaatgccg caagctgacg taagcattct ccggcatgat agtggctggc
     1261 gcgggcgtgg aaggcatcaa cgctggcgcg cagttgccgt agcgattcac tcacccatcg
     1321 ccagctgcag cgttccgctg cttgctgcgc gcggctgaaa gcggcctcgt agtgaataag
     1381 cggctggctg atgccgctca gccataatgc ctggcttagt tgttgaacat attgacgaca
     1441 cgttttgcct tcttcgctgg caaacggatc gtcagatgat gtgacgtgtt cgctgcgcat
     1501 ctgccagatt aaatgagtca attccgcttg ctgcgctttt gcctggacaa aggcctgtac
     1561 tgccagtacg acatgttcgc aaagtgtgcc ttcaatacaa tcacaacggg cgaaacgaat
     1621 actgctgcgg gaataaaaac gcacatcgct catcggtaag cgggcagagg gaatttcacc
     1681 cggcgcacag aacaactcaa tggtgatgct tttagcgacc agcgcctgtg cgcgtttgcg
     1741 ggtggcatcg ggaagggtag ccagttcttc cagccagatt gccggatccc attcctcttc
     1801 ttttcccgta ggctgggcgg tagcacaaag tcgttgataa cttaacacca gcatcacgcg
     1861 atgacggcac atgccgctgg ccccataggt gcactgagcc tctttcagtg cctggccgtt
     1921 cgccagctgg gtacggacac cgtcactgaa ggtggcgatt aaagcgctgt tctcatggct
     1981 gatttccggg acgttgccat tttccagttc cttaaggctg cgcttaacaa aaccggcatt
     2041 gcttaacgcc gtcagtgcct gcggtgtcag ttctaataat tccggacgta gtgaattcat
     2101 gactgaagat tctccgcaag ccatgatgcc agctcgcccg gtgtcatggc ggctatttgt
     2161 gcgccgacat taatcagcgc ctgggccgta tcgcggtcat agcaaggtgt tgctgtgcta
     2221 tcgagcgctg ccagtcccag cactttgatg ccgctctgga cacacttttt cacttggtgc
     2281 gtcagcaatg atgatgaacc cccttcataa aaatcgctca cgaggataat gacgcttttt
     2341 gcaggttgtt caataagttg ccgaccatac tccatggcac tggcgatatt ggttccgccg
     2401 cctaattgca ctttcattaa taactctacc ggatcggcaa cgtctgccgt gagatcaacg
     2461 acgcttgtgt caaacgccac cagatgggta cgaatgccgg gtaactgcca taaacaggcc
     2521 gccatcaccg cagagtggat caccgaatcg accatcgatc cgctttgatc aaccagtaag
     2581 accagttgcc attgctcgct ttggcgttta atgcggctgt taaagcgggg gaattcgata
     2641 tacaacttgc cgtgttgcgg gtgccagtgt tgcaggttgg cgcgcagagt acttttgaaa
     2701 tcaaagtttc gcgccagtgg aataaatgag cggcgacgac gatcgcggac accagaaaaa
     2761 gcctgacgaa cttcctttgc cagtcgcgcc ataatttctt ccacaacctg gcgcacaatc
     2821 ctgcgggcgg cagccagtac ttcggggttc atcagatgtt tggtgtgcaa aacggcgcgt
     2881 agcaggcttt cggaaggctg catacgttcc agcacgtcga gatttgtcac cacatcttca
     2941 atgccgtagc gcagtacggc atcgctttcc agccgctcaa tcacctgttg cggaaacagc
     3001 gtgtgaatac tgttgatcca ctcaggagtg gtgagatttg agccacctaa tccactagag
     3061 cgttcaccac gctggagccg ttcaggatcg cgcccataca gccactccag cgcgtgatct
     3121 atctgccggg cgttgtcatc cagcccacaa agcgtcgttt ctgccgcttc gccaagaatt
     3181 aatcgccagc gttgtagctc acgggtggtc agaagatcgt tcagttcaga catggaaaac
     3241 tccccagtgt tgcagcgatg ccagtgcctg ctgttcgagt tgttgatgat gtgcaatggc
     3301 ttgcggtgga caatgcaacg gcatttgcag tgccgaaacg ggaagttgcg ccagttgata
     3361 atgctcaagc acctgatgcg ccagcgtccc gcgttctcgt ggcggtagcc aggccattgc
     3421 cgcgcgtaaa tcgggcaggg cattggtaaa atcggcatca cttagttgat ttaaatgact
     3481 gctgaaaccg gcgataaatg ccggctgaca ggccagttga tgacgggcta atgccagcaa
     3541 accgtgcagc gcctcgccgg attgtgccgg ggataactgc gccagcatcg tcagcgccgc
     3601 ttcggcacta gcgttgggat gttccagacg catcagcgca ccaagagtcg cgccgcgatc
     3661 cagtgccagg acatgaatcg cctgactgtg tcgctccagc aaggctaccg ccgcagaaag
     3721 cgaaacgccg gggagttgca caccgctatg tagatcgcgc aggatatgac acaacgcttg
     3781 ccagctatgc aagtgagcgt gaaactcctt ttcatccggt ctgccgttgg attcacacag
     3841 ccacagcgtg cgatcgatag ccgcgcataa cgtcgtttgt aatatctgcg cgccttgcat
     3901 accgctaatt tcatccagcc gccataaggc atatagcact tccagcgcct ggcccatttc
     3961 ggcaaattga ttttcctggg cgattaataa tgtgagttgc tccagtaatt gttgactgaa
     4021 ggacgccaga cccgctgacg ccgcttggct aagacatgtg gtaatgctgc cgattccacc
     4081 cgcgtccagc atatcggctt ctaatttatg gcgtgcggct tcctggagtg tggcaccaaa
     4141 gcaggcggcc tcaatcaatg ccgcatgctg gctaagcggg cgggttaatt tccagcgttc
     4201 ttcaccgttg cctgcaagtg tcagtgtgct tccctgctgg cgtacaatcc ctgggatctc
     4261 cagtattgcc agccgatgta acacctgact ttgcgctagt ccattggggt taaagcgatt
     4321 taaggtaagc tccgccggaa gagagattgc ggtacgttcc agttcgcaag tgacatcttt
     4381 tggcagaggc ggttgtggcg tagaaggggc aagtttaccg aatccgtcac ccgctaatgt
     4441 gtctatcaac gttagcagaa tcggatcggt atcgggatga ataacgccgc gatagctcca
     4501 cggcaacggc gcattcaggg cttctttaat cagcgatcct gctatggcat ccagccagtc
     4561 agtgcgtaac ggtagtgtat gaccgcgcaa ctgtgccagt gccatcgcat gcagatgagc
     4621 ggcagccata tccgcggtcg aagcaggcaa cttgtgctgg cgcaaacggg tgagaaccgt
     4681 ttttagcagt tgttctcccg cctgctgtaa gccccactgc cagcaccagt tttgccagac
     4741 cggggcaggc attcctgaaa ggtatcctgc cagcacatca aggcgctttt cactgtaggg
     4801 ggtgagataa caacctgtaa cggcatctgc cagcgacggc aattctggcg tgttaattac
     4861 ctgcggacat tcgcgccaca tttttgccag tgccggagcg tgccagccac cgcagacaac
     4921 taacacgtcg ccattatttt gctgcatcgc ccatccaatc cagcgggcca taaaggcttc
     4981 gcgctgacga tttagagcat cgccggggga gtcgccccgc agttgggcaa aatagtgcgc
     5041 cagtgcagag ggtaatgcag tttgctggct ttcattttcg aacaagtgat cccacagggt
     5101 gtcgctgtta tccatgcggg tggcacgcag cagcaaggtc tggctttcct cttgcgtatc
     5161 aggcgaatcg tcctcttctt cactctgcgc ccagcaaggc aaatcgatga agtaagtttg
     5221 tgcctgaata cgacgatcgg cttgtagcgc ctgccactcc ggcgaaaatt cagcaaatgg
     5281 cgtccaggca ccacgcccgg gggctgcacc gtcctgatac tggcaataac tgtaaatagc
     5341 taccggaagc tggtgggcta aaaacagttc gtctacccgg tcattaaaat cagcagggcc
     5401 ttcaatcaac acgtatcgtg gccgctggct ttcgattaac gatttcacca gccgggcgca
     5461 ggccggacta tgatgccgga tgccgacaat taacggctcg ctcatgcatc tttcctcacg
     5521 gcaggcggtg gcgggcttga taataagcct gccagtgagc ttctttatgt gtggcgacac
     5581 gctgttcgaa gtaacggcgc agacgagcgc gatcttcttc gttatctttg acgatggttc
     5641 cggcaatgca gtccaccagg tcggctggct cacctgcgcg attcgctaaa aaccaggcgc
     5701 ggacgcctac ggcgtgggcc acgttcacgg cttcagcggt ggacataatc gctgttagtg
     5761 tatccattga cgttttcttt tcgccgttgg cacgcagatc gcggaaggtg cggaccagta
     5821 actccagtac cgcgtcaggc actttatgcg gaataccgct atgggccagc aaacgcgccg
     5881 aagcactggc gacaagctcc agctcctggg cgaaatccat aatcggaaag acagtttcaa
     5941 aatcgaagcg acgctttagc gcagcactca tttcattgac gccgcgatcg cgggtattgg
     6001 cagtggcaat aatattaaag ccttctcgcg catagagctg actggcttca ccagtgagtt
     6061 ccggcaccgt catcaccctg tcagaaagca tacctaacag acagtcttgc acttccagcg
     6121 gtgtacgcgt tatctcttca aaacggacga ttttgccgtc gcgcattccc tggtaaagcg
     6181 gggcagggac cagcgcttcc gttgatggac catgattgat aagcagggcg tagttccagc
     6241 cgtacttgat ttgatcttcg gtagtagatg cgccgccctg gatggttaac ccggcatcgc
     6301 cgctaataga ggttgccagt aattcagaca ggagagattt tgcggtgcca ggttccccca
     6361 ccaacatcaa accgcgtccg gtggcaagag tgactaacat acgttcgaca gcggagggac
     6421 taatcaccac tttacggcta atattttgcg cgctatcgcc aaggataaaa gcacgggccg
     6481 caggcaaact taactgccaa ccgggcgggc aaggtgcgtt atcattttgt tttaattttg
     6541 ccagttcatc ggcgtataac acagcagcag gcggacgctg cagatgatta ttctgcggtg
     6601 acatatattc atccct
//
LOCUS       DAEEGA010000155         6363 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEEGA010000155 DAEEGA010000000
VERSION     DAEEGA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.156"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            58..1384
                     /gene="pgaC"
                     /locus_tag="KLH46_003827"
                     /pseudo
     CDS             58..1384
                     /gene="pgaC"
                     /locus_tag="KLH46_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
     gene            1386..1799
                     /gene="pgaD"
                     /locus_tag="KLH46_003828"
                     /pseudo
     CDS             1386..1799
                     /gene="pgaD"
                     /locus_tag="KLH46_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
     gene            complement(1849..2637)
                     /gene="phoH"
                     /locus_tag="KLH46_003829"
     CDS             complement(1849..2637)
                     /gene="phoH"
                     /locus_tag="KLH46_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBE0380331.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3245..4510)
                     /gene="efeB"
                     /locus_tag="KLH46_003830"
     CDS             complement(3245..4510)
                     /gene="efeB"
                     /locus_tag="KLH46_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBE0380332.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4516..5632)
                     /gene="efeO"
                     /locus_tag="KLH46_003831"
                     /pseudo
     CDS             complement(4516..5632)
                     /gene="efeO"
                     /locus_tag="KLH46_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
     gene            complement(5690..>6363)
                     /locus_tag="KLH46_003832"
     CDS             complement(5690..>6363)
                     /locus_tag="KLH46_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBE0380333.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1533 a         1676 c         1531 g         1623 t
ORIGIN      
        1 accaacctga aatagacctt attcgtcctg agttttcaac agcctggtat ccgaaaaatg
       61 attaatcgca tcgtatcgtt ttttatatta tgtctggtgt tatgcatacc cctatgcgta
      121 gcgtactttc actctggtga actgatgatg aggttcgttt tcttctggcc gttttttatg
      181 tccattatgt ggattgttgg cggcgtctat ttctgggtct atcgtgaacg ccactggccg
      241 tggggagaaa acgcaccagc tccccagttg aaagataatc cgtctatctc cattatcatt
      301 ccctgtttta atgaggagaa aaacgttgag gaaaccatac acgccgcttt agcacagcgt
      361 tatgagaaca ttgaagttat tgccgtaaat gacggttcaa cagataaaac ccgtgccatc
      421 ctggatcgca tggctgcaca aattccccat ttgcgggtca ttcatctggc gcaaaaacag
      481 gggaaagcca ttgcgcttaa aaccggagct gccgcggcga aaagtgaata tctggtgtgc
      541 attgatggcg atgcgttatt agaccgcgat gcggcggcat atattgtgga accgatgttg
      601 tacaacccgc gtgtgggtgc cgtaaccggt aatcctcgta ttcgaacacg ttctaccctg
      661 gtgggtaaaa ttcaggttgg cgagtattcc tcaattattg gtttgatcaa gcgaacccag
      721 cgtatctatg gaaacgtatt taccgtttcc ggtgttattg ccgcatttcg tcgcagcgcc
      781 ctggcagaag tgggttactg gaatgacgat atgatcaccg aagatattga tattagctgg
      841 aagctgcagt tgaatcagtg gacgattttt ttacgagcca cgggcgctgt gctggatatt
      901 aatgcctgaa acgttaaaag ggctgtggaa acagcgcctg cgctgggctc agggcggtgc
      961 agaagtattc ctcaaaaata tgacaaggtt gtggcgcaaa gaaaactttc gaatgtggcc
     1021 gctgtttttt gaatactgcc tgacgacaat atgggccttc acctgcctgg tcggtttcat
     1081 tatttacgca gtccaacttg ccggtgtacc gttaaatatt gaattgacac atatcgctgc
     1141 gacacatact gccggaatat tattgtgtac gttatgttta ctgcaattta ttgtcagcct
     1201 gatgatcgag aatcgctatg agcataatct gacttcatcg cttttctgga ttatttggtt
     1261 cccggttatt ttctggatgc tgagcctggc aacgacattg gtatcattta cacgagtcat
     1321 gttgatgcct aaaaagcaac gcgcccgttg ggtaagtcct gatcgcggga ttctgagagg
     1381 ttaatatgaa caatttaatt attacgaccc gacaatcacc agtacgttta ctggttgatt
     1441 atgttgccac aaccatcttg tagacattat ttgctttgtt catattctta ttcgccatgg
     1501 atctgctgac gggttattac tggcaaagcg aggccagaag ccgacttcag ttctattttt
     1561 tgctggcagt ggcgaatgcc gtcgtgttaa ttgtctgggc gctgtacaat aagctgcgtt
     1621 ttcaaaaaca gcagcatcat gcagcctacc aatatacgcc gcaagaatat gcagagagct
     1681 tagcaatacc tgatgagctc tatcagcaac tacaaaaaag ccacaggatg agcgtacact
     1741 tcaccagcca ggggcaaata aaaatggttg tttcagaaaa agcgctagtc cgggcataaa
     1801 cacccaaaac aaagcccggt tcgcccgggc tctgcaccga taatacactt aactgtatgc
     1861 atgcagcgta cgttggcaaa gtgccgaacg tacgcaatcc tcttttccga accggacgat
     1921 cccaaccatt tcatcttctt cgaaacgttc cagcgcgtca cttaatccgg agcacacgcc
     1981 gcgaggcaaa tcgcattgcg tgatatcacc gttgacgata accgtcacgt tctccccgag
     2041 gcgggttaaa aacattttca tttgcgcggc agtcacattc tgcgcctcgt caagaatgac
     2101 aactgcattt tcaaaggtac gtccacgcat ataggcgaac ggcgcggttt ccaccttccc
     2161 tatttccggt cgcaggcagt actgcataaa ggaagcccct aagcgccgga ccagcaagtc
     2221 gtagaccggg cgaaaatagg gagcaaactt ttctgcgata tcaccaggta agaagccaag
     2281 atcttcatcg gcttgcagaa ctggacgggt gacgataatc ctgtcgacat ccttatgtat
     2341 cagggcctct gccgcttttg ctgcgctgat ccaggttttt ccgcacccgg cttcgcccgt
     2401 ggcgaatatc agctgcttac tctcaatagc ctgcagatag tgcaattgcg cttcatttcg
     2461 cgcaaggatg ggcgaggtat cgcgactgtc gcgggccata ccaatggctt ctacgccgct
     2521 catctgcaca agcgaggtga ccgattcttc ttcacgctgc ttatggctgc gcgaatcccg
     2581 tctcagcaca cgttttgcct cgcgacgagc tttgatcact gctttttgtc ttcccatgga
     2641 gagcaccttg agttgtttgt attcatcaca cgcgccgttg gcagcgcgat tatgcgcacg
     2701 aacatcagag ggttggcttc cttgtaagcc atagtttgct tttggataaa atgccgaaaa
     2761 acggctacgc acaccgtttg cggcgtcggt aacacgagta aagaaaggaa gtgagaaaaa
     2821 tacagagtga cgaagttgct accggaggag aaatctccgc gagtggtgga gcgttgctta
     2881 acaaaacatt gtccagaaca ggacgttacc atccgcgatc tccatagtga ctgactatca
     2941 ctgccgggaa cttccgctgc tacttaataa gtacaacaga actcgcattc attgcaacaa
     3001 tatattcact tatatttaac gataaacacc catttcggtg acattaattt ctactggaaa
     3061 gatgacagag tgatgacagt gatggaaaaa gctgtgtgct ttccgcagga ttcgcagggc
     3121 tggtaaaatg tgcctcgtgt ttgccggatt cggcgcgaac gccttacccg acctacaagt
     3181 gattaataat ttgtgcacaa aactcatgcc cgataaacct gcgcatcggg catatttgcg
     3241 tcgttcaaac ctgcaatagc gcccttccca gataatcgtt cgcgtccttc actcccggca
     3301 gcgaaaaaaa ataaccgccg ccgataggtt taacgtattc ctccagcgct tcgccattga
     3361 gccttttttg tactgtcagg aagccttttt ccagatcgtg ttggtagcag acaaacagca
     3421 accccatatc cagttgtccg gagttggtga cccccaacga ataactgtag ccgcgacgca
     3481 gcatcaggct ggactcactc tccgccgtgc ggggattcgc cagccggata tggctgtcca
     3541 gcgcgatcac cttcccttcc gggtcgctgg cgtaatcagg cacatcatgc tcatgctgca
     3601 ttcccagcgg cgcaccggtt tgcttatcac ggccaaaaat cgtctgctgt tctttcagcg
     3661 gcgttctgtc ccaaaattcc actcgaaact gaatcaagcg tactgcctga tagctgccac
     3721 cgattgtcca cgcaggctcc tgctgatctg ccgttaccca caccactttt tgcatcaact
     3781 tatcattctg gctatcggga ttggcagtgc cgtctttgaa acccagcaaa ttaatcggcg
     3841 tctctttgcc tttactacgc gccgcgtgat cggaaataaa cccttcccgc ttccagcgca
     3901 cactgagcaa atccggcgtg tgtttgatga tatcgcgcag cgcatggttc accgtgtcct
     3961 gggtgttggc gcaaatctgt agcaacacat caccatgaca taacgccgca tccagcgagt
     4021 cgttggggaa acgcgtcatc ttctgcagct tttttggcat ctgtggcgca aggccaaagc
     4081 gctcatcaaa caatgagtga cccaccgata acgtgatggt gagattatcg ggcgcaatgt
     4141 agccgccaag aatgccggaa tcgagtggtg gcaggcgcgg atttggcgtt tctggtgctg
     4201 ctccgccctg actcagaaaa gcaaaacgct gagtcaacaa acgaaacaac cgctcaagat
     4261 cggctttatc gctggcaagc acatcaaacg ccaccagcat cattgcggcc tgttgtggcg
     4321 tcaggatccc tgcctgatgc tcaccataaa acggctgttt ctcattgcgt gcatccggtg
     4381 aaagcgtgcc cggtgcactt tgcgtttttt gtgcatgagc gaccggacaa cttcccgcca
     4441 gcgcaccaat ccctttcagt aaacgtcggc gtgacggttc attcacgccg ttttcatctt
     4501 tatactgcat aacgcttaat ccagtcccag cacaccgcga agttgcgcca gatcttccgc
     4561 cagcgcagta atcggccctt tcagcgcatt ccggtcggca tcggtcaatt tgtcgtaggt
     4621 ttcaaaaccg tctttagtac ggtatttcgc cagaatggta tcgacctttt taaagttggc
     4681 ataacagttc cgggttggct ttttgcagtt gtggacgcag cagatcgaca attttctgcg
     4741 agccttcaac gttagcctgg aaatcccaca gatcggtgtg gctgtagcga tcttcttcac
     4801 cgctgatttt gctggctgcc acttcctcaa tcagtccggc tgcgccgccg accacttttg
     4861 aaggtgggaa agccagttca ctgatgcgtt tttgcaaatc gaccacatcg gtataaagct
     4921 gctcagcgta ctgatccatc cctttggtgg tgttgtcgcc aaacaatgct ttttccagac
     4981 ggtggaaacc agtgaatttt gggtcggcgg ctttttgctc gtaatcatct tcacgggcgt
     5041 caatgctgcc atccagatcg gagaacagtt cagcaatcgg ttcaatacgc tcatagtgct
     5101 ggcgcgtcgg cgcatacagt gctttcgctt tttcgatatc gcctgcttta atcgcgtcgg
     5161 taaaggcttt ggtgtcggtc accagctgcg tggtttccgc catgacatac gctttatatg
     5221 cagtaattgc accaccaaga cttaacagcg catcactttg cgccgcatcc gccgttgcct
     5281 cacctttgac gatcaacttc cctttcgggt tagtcagcag accgcaggtc atatcgtatt
     5341 cgccaggctg taaattcgcc gtcattttct ggctaaaacc aggggcgata ttttcccgct
     5401 cttccaccac catcacgcct ttgaggatct cccactccag cgccttctgg ctatggttct
     5461 gaataatgaa ctgtgttttc ccggcgttaa ccgtaatggt catcggttcg cactgcttat
     5521 ccgtcacggt cactttgacc tgcggcacat cagcggcgtt agccataaaa gcagaagaaa
     5581 acagcgcagc cacgctcaac tgcaatgcgt tacggcggaa gttaatggtc atgacgagac
     5641 tatcccttta aagtatgttg taactaagca agagtttgcg tcgtatttgt tacgcggagc
     5701 gagacgctgt cgcccctgcg cgtggtggca gagcaaatgc caccagcgcc gggatgagat
     5761 aaataaacca gacggcgact tcgctgacgc tcggtgcttc ctgatagcca aaaatccctt
     5821 ccatcagcgt gccaaacagc gagtgagttg agaggaccgc gctcatatcg aaggcgattt
     5881 cctgaaagtg gttccacaat ccggcttcat gaaatgcgcg aatggcacca gccgccagcc
     5941 ctgcggcgac gaagagaata aacaggctgg tccatttaaa aaatgcacca agattgaggc
     6001 gaataccgcc ccagtagagc aggaagccga gcaccacggc agtagcaaga ccgagcattg
     6061 cacccagcgg cggccagatc ccgacatctt gttgaaatgc cgccagcagg aaaaagaccg
     6121 actccagccc ttcccttgca acggcaaaaa agaccatcat caccagcgcc cagccatgat
     6181 gatttccacg ctgcaatgcg ctatcgactg cctgttccag ttgcactttg acgttgcgcg
     6241 acactttgcg catccagaaa accatccagg taaggatcac cacggcgatc accgccacga
     6301 taccttcaaa cagttcctgc tctttttgcg gaaattcgcc ggtggtttcg ttaatgaaga
     6361 tcc
//
LOCUS       DAEEGA010000156         6355 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEEGA010000156 DAEEGA010000000
VERSION     DAEEGA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.157"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..370
                     /locus_tag="KLH46_003833"
     CDS             <1..370
                     /locus_tag="KLH46_003833"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380334.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="KLH46_003834"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="KLH46_003834"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBE0380335.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="KLH46_003835"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="KLH46_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HBE0380336.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="KLH46_003836"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="KLH46_003836"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HBE0380337.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="KLH46_003837"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="KLH46_003837"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBE0380338.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="KLH46_003838"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="KLH46_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HBE0380339.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..>6355)
                     /locus_tag="KLH46_003839"
     CDS             complement(5095..>6355)
                     /locus_tag="KLH46_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBE0380340.1"
                     /translation="TELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRD
                     IVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHICEIAGGNLA
                     NTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTE
                     QQASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEI
                     ADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQ
                     AAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGI
                     DQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQT
                     PSRPASEQPPAQPRLRITEQDPNWETF"
BASE COUNT         1439 a         1837 c         1525 g         1554 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgat ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaggaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccagc gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgcctgg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagtt
//
LOCUS       DAEEGA010000157         6331 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEEGA010000157 DAEEGA010000000
VERSION     DAEEGA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.158"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            5..436
                     /locus_tag="KLH46_003840"
     CDS             5..436
                     /locus_tag="KLH46_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBE0380341.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="KLH46_003841"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="KLH46_003841"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBE0380342.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="KLH46_003842"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="KLH46_003842"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBE0380343.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="KLH46_003843"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="KLH46_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBE0380344.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="KLH46_003844"
     CDS             complement(4438..5145)
                     /locus_tag="KLH46_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBE0380345.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="KLH46_003845"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="KLH46_003845"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBE0380346.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="KLH46_003846"
     CDS             complement(6003..6206)
                     /locus_tag="KLH46_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE0380347.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1405 a         1810 c         1648 g         1468 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc caccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct t
//
LOCUS       DAEEGA010000158         6269 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEEGA010000158 DAEEGA010000000
VERSION     DAEEGA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.159"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..667)
                     /locus_tag="KLH46_003847"
     CDS             complement(<1..667)
                     /locus_tag="KLH46_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HBE0380348.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(657..2414)
                     /locus_tag="KLH46_003848"
     CDS             complement(657..2414)
                     /locus_tag="KLH46_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HBE0380349.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QWIDVWGIFTVSMAVRRDN"
     gene            complement(2430..3314)
                     /locus_tag="KLH46_003849"
     CDS             complement(2430..3314)
                     /locus_tag="KLH46_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBE0380350.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(3325..3930)
                     /locus_tag="KLH46_003850"
     CDS             complement(3325..3930)
                     /locus_tag="KLH46_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBE0380351.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILFICTAINRCRSVLLMSAER"
     gene            complement(4101..>6269)
                     /locus_tag="KLH46_003851"
     CDS             complement(4101..>6269)
                     /locus_tag="KLH46_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HBE0380352.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1837 a         1568 c         1315 g         1549 t
ORIGIN      
        1 atggcagtgc tattttgatt cgacgttgat caagtttctg ataattccaa cgacggtcga
       61 cgggcaccat ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat
      121 gatgctgcca tgtcgtcagc gtcgaaatgg cgatgagtaa aaaccatccg ccacagactt
      181 tacgccacct cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaataa
      241 tatgcagcga aggagactgg ttataaggca gatcaaacag ttcaagttgc gaaaatagcc
      301 atcctgtcac cccactcact tcaggacgga taaaactaaa cttcagcggg tagagaaaaa
      361 aaccgcagca ggccattacc gttgccagaa taagccggtg gacaagtcgg cgttgttcga
      421 atgtcgagct acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggaa
      481 caatagtcca gggaagaaaa gggatcgccg tttcccaacc gaatacctga ctggggatat
      541 catggctgtt aaggtcctga accgcggtga actgattaag agatccatag gtgaagaaga
      601 aaaacggggc cagcaacaat aaccagccag cgccttgtag caatacgttc cgacgttcag
      661 ttatcacgac gcaccgccat cgaaaccgta aaaatgcccc acacatcaat ccattgagtg
      721 catttatcaa atccggcatt acgcacgagc gaatccatct ccccttgcga acgcacgcgc
      781 attacccacg gtttaccatc tttatgactg gttaacaccc cggcaatcag ctccagttgt
      841 gggtgccacg gctgcccggt gtagatgaga atgccacccg gttcgatggc attggctaga
      901 cctgcgagtg agttttttac ctgctcattt tcgggaaaaa gctcatatag gccagagaca
      961 atcgccagcg taggccgcgg agttaacgcg ctgagttcct ccgggttaaa cgcatcgccc
     1021 tgttcaaaac gcacccgccc agacattccc cgttgggcaa tcatctcttg cccctgtgca
     1081 acatttaact cactgtaatc acgtaacaaa atatcgctta cggcaggctc gtttgccagc
     1141 gcatccagta catagcgtcc atgccctgcg gcaatgtcaa ccacgcggac ggctaaacct
     1201 ttggcgtgga gatcggcaac ggcttgttta atcaatattt gcaaatgggt tttgcgctgg
     1261 cgaataccgc gccatccaac actgttcagg tagattttgt cgattaaacg cccgaatgcg
     1321 ttactgcctt gcggttgatt ttgatagaca taatccaggg aactgcctga atcaaagccg
     1381 gtactcattc cgagatgcag gcccgcagag tgcgcaccga acaatttcat cgcctttcgc
     1441 ataaagcgat acgctaaatc aaccggcgat aatggcacgg gtccaccaga aaggagccgc
     1501 catcgatccg ctgatggtcc tgtgcggtct tcatgctgat aatcaaattt ttgcgatttg
     1561 ttagcatata agcggcagat aaagctttgc attttttcaa atgcctgcgc cctgttctct
     1621 tcacccaatg tgtcgtgata aaagcctggc agcagatgca gctctttcag agggctacgt
     1681 aatctctgat aaaaatcaat ctgcggttgg cgatgcacca catagtcatc acctgatatc
     1741 agaagttgcg tggggagcgt aatcgccgcc gcatcgctaa caatacgttc agacgtttta
     1801 tagagatcga gcaagatatt gacggcaatc gcccgtgtga tcagcggatc attattgaaa
     1861 ctcgccaccc gttgccgatc gtgggtcaaa tagcgccctt tcacatagga attaataaaa
     1921 aatagaccac gcagacgatg ccataacgcc agcgcaggac gtgccagcgg cacatacaat
     1981 ttaaccttaa aggccggaga agccagcacc agcccacgaa ttgcaggtgc ataatcatga
     2041 acccatgtgg caacaagcac tgcgccgacg ctttgcgcga tcactaccac ctcttccagt
     2101 ccgacctggc tgtcgctgac agcaaaacgg acaaattcat cgacatcctg cactgagcgc
     2161 ataagagacg ggctataacc acgcggcccc gaattttttc catgcccccg ggcatcccag
     2221 gcataaaaag cagtatcagg catcgccagt tcatcaacga gatgttgtag acgaccagaa
     2281 tgttcatgcc cgcgatgaaa taagacgatg acctttttcg cccctggctg taaagtgggc
     2341 cagtgccgat aatacaacgc tgtattatca ctggtggtaa aaaaatgttc cccagggatg
     2401 cgtgaatttt ccattttctc tattttcctt cagtaacagc agtagcgaat aaaataaaaa
     2461 aataccggag cggtgaaaat taatgagtca atcctgtcaa ggagtccgcc atgtcctagt
     2521 aatagttttc cactatcttt aacaccaata tctcgtttga tggctgacat cactacatcg
     2581 ccgcaaaaac cactaatacc aattaacaaa cccgctaata atgcctgtaa tgtatttagc
     2641 ggtgtcagca acggtccaat aatcagtgac gcaatcatgg tggtgattac gccccccagc
     2701 agaccttcca gcgtttttcc cgggctgact ttggggacca cttttcttct gccgcaggat
     2761 ttcccccata aatactgtgc aatatcgttt gactcggtta acgccaaaag aaataacacc
     2821 agtaacgccc cgccctggat atttatggtt ggcaacatta atagccaggc ggcatgactc
     2881 aaggcaaaaa cggtcattat ccatccccaa aaaatggcgc tcacggtatg ctgaaaaccg
     2941 gaggggtcac ccacaaaaac tcgccaggtg gctaatatca gaaacccagt caaaggtata
     3001 aataacagaa acagctcaaa gcaattaaaa ccgataagca aatagttaag aggaataacc
     3061 caatataacc aacgcgaaaa atgtacggat ataagcgtac aatattcttt caatgccaga
     3121 aaacttatca gcgtaaaaaa cgtcaacgtc atccagcgtg ggcctgaaat caccagtgaa
     3181 aaacaaatga cgatgcccca ccaggtacga atacgtaatg taacttcatc cccttttctc
     3241 tcaggtaacc gccatagcag aaaacggttt atcagcttgc taaaattaac aacgcgaaaa
     3301 gtgttgccag agatttttcc agcattatct ttcagcgctc ataagaagca cactccgaca
     3361 acgattaatc gcagtacaga taaacagaat tgaagcaatg ctccataaaa gattattcca
     3421 ttgcatccac tgtggataaa tggcaacggc cagaccccac agaccaaata ttaacgcgcg
     3481 atcacttttg ccaaatggac cggcataact gcgaacaccg ttaatcgtct gcgcgagtaa
     3541 accgcaaaac tcggtcaata tggtgcaaaa taacatgagt atcacgagtg atgcgttact
     3601 ttccggtaaa aataaaaacg gtaaatagag cgcaatatcg gaaataacat cgccagtctc
     3661 attcaaaatc gcccccagac gtgtttgctg attgcactca cgcgccaaca tgccatccag
     3721 cgcattgagc gccatacgga taaaaagcac gatgggcaat agcaaaaaga ggatgggttg
     3781 tgccaccaac accaacagca atccggtaaa aagagaaagc gccagtgccg taagagtgat
     3841 gtgattcgct gtaacgtggt gcttataaag ccaaaacatc gtcggcctta acagcgactg
     3901 aaagagcggt tttatctggt agagcgtcat tatcaatcct tgataattcg gtttgcgcta
     3961 tggtgcatca attcccctga cggagatatc gcaacacgcc gcttcattaa aaattcagaa
     4021 tatatcaagg tattgggggc tcactgtcag gaatgattat ccccaataaa tcaagtagtt
     4081 aaaaattatt acgctgttat tcaatacaac tcatcaagtt tcctcatcgc ttgttcccgt
     4141 attgcgcgcg tcttaccgtt aagcgccagc tggcggacta aatccagttc agggttaaca
     4201 agctctaacg ccagcaaagg atcacgtttc agcctcgcct tcaatacatc aactggaaag
     4261 cggggattat tcatcgccac aatccaccac tcgggatcaa tttctgccgc cagtgccgca
     4321 gagggggtgt ggcggttgct aagtgcgccg aacttgaaaa atgtgagatt gcttttcgcc
     4381 agtgtactcc accatatacg atccggttgt tttgccagta catccagttg tgctaacgca
     4441 tcggggtcga gtgcataata aacacgtaat gcttcatcaa cctgcgcacg ggagctattt
     4501 tcctgcctca gaaaaaactt cgcccaaata tcattgcgtg gcgtgttttg ctgtaattcc
     4561 ttgagaacaa cagcgggtaa attatcccaa ctaactaatg cactctctaa aaagtcccag
     4621 tcttccaaac gtatcagggc ggcggcattt aatagcatct ggtcatcaac ttcgccgtgg
     4681 ctaatcagct cagacaaaat catatcagcg ctggtttcat atgcatctga atctgctttc
     4741 ggattatttt tccgatgcga taaagcacgt tgccaaattt tttgtttcat cgacgtgctc
     4801 agatactgat ttttcgccag attactccac ccctcaggat cgttgaaacg tgtcaataac
     4861 cgttgtaaag cagcgtcaga gtgaattacc tgcatatccc gacagcaaaa gtaggcaggg
     4921 gattttttct ccatcctttt aacaaaatta aactgtaagt tatctggcaa tgctaaaaat
     4981 aacacacgta ttagatcatc gtcattctcg aatttttggt ataaataggc tgcaactttg
     5041 tcactctctt gtggcgtcat ccgcaataat tgaccatggt gtttcatctc tgccagcttg
     5101 aacgctaagc ttgtgacaac attgtttgat gaatcgttcg ccaactgcat tgcctgattg
     5161 taagtgagtt gtcctccagc accgacctga cgaatagagg aatcatcact gcggatatag
     5221 ccatcaataa tggattcggg caataaccac gcatctttat aaagctcctg aatgatcata
     5281 tcccaatctc ttttcgccag agtcatgacg atatccagag ggagattttc acgtagcgca
     5341 agattcaacg gaccgacacc atcaggtaga cgactcattc gcgtcattat ttcgacagag
     5401 agcggttcac ggtcgagcaa ccccagaaca acaggctggt aacccaattg ctgatcggtc
     5461 aactccagaa tctcattgtc aggcatatat ctcgccatac tttcaatcgt ttgttcatca
     5521 ttgctattaa taagttgttt gcgtaggtta gctgaaattc ctttaagcct tgggagagtt
     5581 cgcattatgg caagatcaaa tgtcgcaata tctcgtcctt caaatgcagc aacgttgcct
     5641 tcatccagca tttgcaattc ctgcactaac tcaggagaag gcttagggcg aaacagcgct
     5701 tcttttttca tctgattaca gacattccct gccatgcgaa tattgcttcg cttaaaaatt
     5761 tctttcagag catcttcatc atttattaga ccccaataat atgcatcagg atttattatc
     5821 atcagcgctt ttttgacatt ttcagtgtca tttttgtcac tcgcacggac catgaattca
     5881 ctatttgtcg aagacgcaac aacaggtgca attatgtcat tgataaatac cccacgcgca
     5941 ttaatactcc ccttaatcgt agacggatta tcgtaatatg agtcgataaa taccagtcca
     6001 cttgcattta tcgaaccggt aattaacagt ggtgaatccg taactatatt cgttgccgtc
     6061 acgttaccga gaataaccca tggattagaa agagagaaag tggaaatatt acctttgact
     6121 gtcaaattgc caagaacaat caatttttga ttactctcta tattaccttc taccactaaa
     6181 tcgccacgat gcactttcca gtccgatgca gataaaccga aggggacaag attcacgcta
     6241 ctgttgatag cttgttcaac ttgttgttt
//
LOCUS       DAEEGA010000159         6214 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEEGA010000159 DAEEGA010000000
VERSION     DAEEGA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.160"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..378
                     /locus_tag="KLH46_003852"
     CDS             <1..378
                     /locus_tag="KLH46_003852"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380353.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="KLH46_003853"
     CDS             complement(454..918)
                     /locus_tag="KLH46_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBE0380354.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="KLH46_003854"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="KLH46_003854"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBE0380355.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="KLH46_003855"
     CDS             complement(1787..2476)
                     /locus_tag="KLH46_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBE0380356.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="KLH46_003856"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="KLH46_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBE0380357.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="KLH46_003857"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="KLH46_003857"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBE0380358.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="KLH46_003858"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="KLH46_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBE0380359.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1576 a         1688 c         1547 g         1403 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acat
//
LOCUS       DAEEGA010000160         6210 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEEGA010000160 DAEEGA010000000
VERSION     DAEEGA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.161"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..95)
                     /locus_tag="KLH46_003859"
     CDS             complement(<1..95)
                     /locus_tag="KLH46_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0380360.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYL"
     gene            586..1494
                     /locus_tag="KLH46_003860"
     CDS             586..1494
                     /locus_tag="KLH46_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBE0380361.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKVEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1540..3531)
                     /gene="tkt"
                     /locus_tag="KLH46_003861"
     CDS             complement(1540..3531)
                     /gene="tkt"
                     /locus_tag="KLH46_003861"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBE0380362.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3809..4567
                     /gene="loiP"
                     /locus_tag="KLH46_003862"
                     /gene_synonym="yggG"
     CDS             3809..4567
                     /gene="loiP"
                     /locus_tag="KLH46_003862"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBE0380363.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4773..5693)
                     /gene="speB"
                     /locus_tag="KLH46_003863"
     CDS             complement(4773..5693)
                     /gene="speB"
                     /locus_tag="KLH46_003863"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBE0380364.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5829..>6210)
                     /locus_tag="KLH46_003864"
     CDS             complement(5829..>6210)
                     /locus_tag="KLH46_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380365.1"
                     /translation="VGMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIV
                     NAGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQN
                     ALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1535 a         1616 c         1599 g         1460 t
ORIGIN      
        1 cagagatagc gctgatgtcc ggcagtgctt ttgccgttac gcaccacgcc ttcagtagcg
       61 gagcaggaag gacatctgat ggaaatggaa gccacgcaag caccttaaaa tcaccatcat
      121 acactaaatc agtaagttgg cagcattacc cttttgctga atgtataact gagattgatg
      181 attctggaaa ataatcacta agccgcataa attatcctta ttttattttt agggtaccgt
      241 ccggcggtaa aaataaaata ctcgtgcggt ttaatgaaag caattgggta cgcgagaaga
      301 aaaaccccac aaaccaaaat aataaaacct taaataacaa caatatgaaa ttgatatttt
      361 taaagtcaac tttgtatttg ttatgttcat cacaaaatca acattttgtt tgttattgta
      421 tttgtgatgc atatctctat ttatttattg aatgttcgaa aacatacttt tctaatgcaa
      481 tgttttttcg ctatacccct ttatagattc tgaattctca cctgtcggct gttttctcct
      541 catctcatgt gacatattcc catactttac tcaaggaaga ttgtcatgaa agcacctgtt
      601 tcattaacct catttcgccc acaaaaatct cttgctatcg ccattggcgt gcttgccgtg
      661 gttgtgctgc catttctctc gtattacacc gttaatgaag gtgaacgcgg cattttgctg
      721 cgctacggaa aaatcgtaaa agttgccgaa ccggggctgg gttttaagat cccttttatg
      781 gaaagcgtgg agaaaatttc cacgcgtaat caggctgtgg tctatcaggg attacaggcc
      841 tacagccgcg accagcaacc ggcacaaatg acagtatcag tcagcttcca cattaaacct
      901 tcagaagcag gcgcggttta taccacctac aacactattg aagcattaaa agatcgtcta
      961 attgtccgtc agttgccaac tcagctggag aacattttcg gccaatatac cgccatcagc
     1021 gccgtacagg atcgtacaaa attggttcag gatctgcaaa atgccatgcg caaagccgtt
     1081 gttgggccag tggtaattga cggggtccag attgaaaata ttgatttttc tgacgcttat
     1141 gaaaagtcga ttgaaaaccg aatgaaagca gaagtcgcca ttgccacgcg taagcaaaat
     1201 ctggaaacag agaaaatcca ggcgcaaata gcggtaactc aggcccaggc agaagcagac
     1261 agtaaactgg cggcagccaa agtagaagcg gaaactattc gcgtaagagg cgcggcagag
     1321 gctgaaacta ttcgtttaaa aagtgcggct gaagcagaag caatccgcct gcgcggtgaa
     1381 gctctgcggg ataatcccgg acttgtagcc ctcaccactg ccgaacgttg ggacggtaaa
     1441 ctgcccgata ccatgatccc cggcagcaca gttccgttta tctctactaa gtaaaaaaag
     1501 ggccgctttc gcggtccttc tcacaaaacg tcttactgat tacagcagcg cttttgcttt
     1561 cgcaacaacg ttatcaacag tgaagccgaa ctcttcaaac agcagctctg ccggagcaga
     1621 ttcaccgaag gtggtcatgc cgacgatagc gccgttcagg ccaacatact tgtaccagta
     1681 gtcagcaata cccgcttcta ccgcaacgcg tgcagtaacc gctttcggca gtacggattc
     1741 acggtaagcc gcatcctgct tgtcaaatgc gtcggtagac ggcatggaca ccacgcgcgc
     1801 tttcacgcct tcggcagtca gtttttcgta ggcagcaacc gccagttcaa cttcagaacc
     1861 ggtggcgatg aagatcagtt ccggctgacc agcacagtct ttcagcacat aaccaccgcg
     1921 cgcgatgttt gccagctgct cttcagtacg ttcctgctgc gccaggttct gacgggagag
     1981 gataagcgca gtcgggccgt cctgacgctc aacgccgtat ttccacgcga ccgcggattc
     2041 aacctggtca cacggacgcc atgtagacat gttcggggtc acgcgcagag aagcgacctg
     2101 ctctaccggc tggtgagtcg ggccatcttc gcccagaccg atggagtcgt gggtgtaaac
     2161 catcacctgg cgctgtttca tcagcgcagc catacgtacg gcgttacgtg cgtattccac
     2221 gaacatcagg aaggtggagg tgtacggcag gaagccaccg tgcagggaga taccgttagc
     2281 aatcgcggtc ataccgaact cgcgaacacc gtagtggatg tagttacctg cagcatcttc
     2341 gttgattgct ttagaaccag accacagggt caggttagac ggtgccaggt cagcagagcc
     2401 gccgaggaat tcaggcaaca gcgggccgaa cgcttcgata gcattctgcg acgctttacg
     2461 gctggcgatt ttcgccggat tagcctgcag tttagcgata aactctttcg ctttggcgtc
     2521 gaagtcagac ggcatttcgc ctttcatacg gcgggtaaat tcagccgctt cctgcggata
     2581 agctttcgcg taagccgcaa acttctcatt ccatgcagat tctttcgcct ggcctgcttc
     2641 tttcgcatcc cactgagcat agatttcaga cgggatttcg aacggtgcat atttccagcc
     2701 cagctgttcg cgggtcaggg caatttcagc gtcgcccagc ggcgcaccgt gggagtcgtg
     2761 agtaccggct ttgttcgggg aaccgaaacc gatgatggtt ttgcacatca gcagggacgg
     2821 tttgtcagtc actgcgcgcg cttcttcaac tgcgcgtttg atggatgccg cgtcatgacc
     2881 gtcgatgtcg cgaataacgt gccagccgta agcttcgaaa cgcattgctg tgtcgtcggt
     2941 gaaccagcct tcaacgtgac catcgataga aataccgttg tcatcgtaga acgcaatcag
     3001 tttacccagc ttcagcgtac ccgccagaga gcaaacttcg tgggagatgc cttccatcat
     3061 gcagccgtcg cccatgaagg cgtaggtgta gtggtcgaca atgtcgtggc ccggacggtt
     3121 aaactgcgcc gccagcgttt tttctgcaat cgccataccg actgcgttgg caataccctg
     3181 acccagcgga ccggtggtgg tttccacacc agcggtataa cctacttccg ggtggcccgg
     3241 agttttagag tgcagctgac ggaagttttt cagttcttcc atcggcagat cgtaaccggt
     3301 gaggtgcagc aggctgtaga tcagcatgga gccgtggccg ttggacagca cgaagcggtc
     3361 acggtcagcc caggacggat tctgcgggtt gtgtttcagg aaatcacgcc acaggacttc
     3421 ggcaatgtca gccataccca taggggcacc cgggtgaccg gatttggctt tctgtactgc
     3481 gtccatgctc agcgcacgaa tagcattggc aagctcttta cgtgaggaca ttttgactcc
     3541 agatcggatg atgaagggca cgcccttaac gacttgacga cagcgcgttt tgggctacgc
     3601 cggaaaattt gccaacaatt taccgcaagc cgcgcgtcat gtacatggaa catccttttg
     3661 ccgcttcaga aatctctgga tcatgctcgc atgttgcgca atctgctcgc ccgtccgctg
     3721 cgcttttcct tatactgaga ctgagcgtcg attcacctgc aaacggcgca tttttagaat
     3781 aatcctgacc ttgtgcggaa gagaaaacat gaaaattcgc gccttattgg tagcaatgag
     3841 cgtggcaacg gtactgaccg gttgccagaa tatggactcc aacggactgc tctcatcagg
     3901 agcggaagct tttcaggctt acagtttgag tgatgcgcag gtgaaaaccc tgagcgatca
     3961 ggcatgtcag gagatggaca gcaaggcgac gattgcgcca gccaatagcg aatacgctaa
     4021 acgtctgaca actattgcca atgcgctagg caacaatatc aacggtcagc cggtgaatta
     4081 caaagtgtat atggcgaagg atgtgaacgc ctttgcaatg gctaacggct gtatccgcgt
     4141 ctatagcggg ctgatggata tgatgacaga taacgaagtc gaagcggtga tcggtcacga
     4201 gatggggcac gtggcgttag gccatgtgaa gaaagggatg caggtggcgc ttggtacaaa
     4261 tgccgtgcga gtagctgcgg cctctgcggg cgggattgtc ggcagtttat ctcaatcaca
     4321 acttggtgat ctgggcgaga aattagtcaa ttcgcaattc tcccagcgtc aggaagcaga
     4381 agccgatgat tattcttacg atcttctgca ccaacgcggc atcagcccgg caggtcttgc
     4441 caccagcttt gaaaaactgg cgaagctgga agaaggccgt caaagctcaa tgtttgacga
     4501 ccatcctgca tccgccgaac gtgcccagca tattcgcgat cgcatcagcg cggatgggat
     4561 taagtaaagc ctagtggtgt cgaacgtagg tcagataagg cgttcacgcc gcatctgaca
     4621 ttaatggcac atttgttacc ttgtgcgcat tgccggatgc gatgctggcg catcttatcc
     4681 ggcctacggg tgccgaacgt aggtcggata aggcgttcac gccgcatccg acattaatgg
     4741 cacgttttac ccgtgcgcat cgcatctggt gcttactcgc cctttttcgc cgcctgaata
     4801 tacagcatct ccagcgccag cgtcgccgca gccagagcgg tgatttccga ctgatcgtat
     4861 gccggagcca cttccactac gtccatccca acgatgttga gatctttcag gccgcgtacc
     4921 agtttaatgg cgcgatcgga ggtcaggccg ccaatcactg gcgtaccggt tcccggcgcg
     4981 aaagcaggat ccaggcagtc gatatcaaaa gtcaggtaaa ccggcatatc acccacaatc
     5041 tgtttcacct gggcgataac gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc
     5101 acggtaaagc cgttgtcttt atcaaactcg gtacgaatac caatctgcac ggaatgattc
     5161 gggtcgatca gaccttcttt cggcgcggta tagaacatag tgccgtggtc aaattcacaa
     5221 ccgttcgcat aggtatcggt gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc
     5281 ttcgcatgag cacgcagcag cggcagcgta acaaagtggt caccaccgaa ggagagcata
     5341 cgcttaccgg cagccagcag cttctcggcg tgcgcctgca gcttttcgct catctcacgg
     5401 gcatcgccaa aggcatatac cagatcgccg cagtccacga cgttcagacg ctcacgcatg
     5461 tcgaaattcc acgggaagcg gttgtgttcc caggccagat tcgtcgaaac ctgacggatc
     5521 gctgccggac cgtggcgacc acccgcacga ccagaagtgg ccatatcgaa cggcacgcca
     5581 gtaatcaccc agtctgcatc gctgtcatac ggctggaagt tcatcggcag gcgtaaaaaa
     5641 ccaaaggcat tggaaaccag tgagttatcg tattgatgac ctaaggtgct catggatgga
     5701 ctcctattac aaagtcgata taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg
     5761 gttggatttg cgaaacacaa gtttgtgcta attatcgcct taaatgggcg aggcatcaag
     5821 cgaggttgct atttccgaca cggtggcggc gcatagccat tgttatctgc cggaaaggct
     5881 tgtccagagc acggcggcag cgcgttttgc gccgggcctt gtaattcaaa ggcggcaaag
     5941 ccgattaccc cggtattacg cacatcgcct tgcgccgaat tggctgcgta ggaatcagca
     6001 acgttggcga actgaaaagc cgcttcagta tgtttatctt tgcggaatcc tttaatcact
     6061 aacgaatctc cggcattcac aatatagccg ttgttattca gcgaaccttg cttgccgttc
     6121 agcacatcca ggccatcagc cgtagcaacg atctcgtagt tggtgttacg actgtaatta
     6181 cggacatata attgataatt cattccgact
//
LOCUS       DAEEGA010000161         6057 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEEGA010000161 DAEEGA010000000
VERSION     DAEEGA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.162"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            381..1409
                     /gene="torT"
                     /locus_tag="KLH46_003865"
     CDS             381..1409
                     /gene="torT"
                     /locus_tag="KLH46_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBE0380366.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1382..2074)
                     /gene="torR"
                     /locus_tag="KLH46_003866"
     CDS             complement(1382..2074)
                     /gene="torR"
                     /locus_tag="KLH46_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBE0380367.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2161..3333
                     /gene="torC"
                     /locus_tag="KLH46_003867"
     CDS             2161..3333
                     /gene="torC"
                     /locus_tag="KLH46_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBE0380368.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3333..5873
                     /gene="torA"
                     /locus_tag="KLH46_003868"
     CDS             3333..5873
                     /gene="torA"
                     /locus_tag="KLH46_003868"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HBE0380369.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQAASAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5870..>6057
                     /gene="torD"
                     /locus_tag="KLH46_003869"
     CDS             5870..>6057
                     /gene="torD"
                     /locus_tag="KLH46_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBE0380370.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         1471 a         1516 c         1679 g         1391 t
ORIGIN      
        1 ccatttcggt cccagttagg acgctttctc atcaagccag tccgcccacc actgcatcat
       61 ctccctacgc ttatcgagat actgagcatg gttgtaaatt caattaaccc agtgcgttga
      121 caaaaaatta gcgcaagaag acaaaaatca ccttgcgcta atgctctgtt acaggtcact
      181 aataccatct aagtggttga ttcatagtga ctggatatgt tgtgttttgt cgcgtttttc
      241 agtctattat ttaatcatta agtaatctaa tgctatgatt tttaaattat ttatgtgatt
      301 ttgtcatcct ttaggtgaaa aaggttgagt cgcaaagtgg aatgcatcta gcataaagcc
      361 ttattattga tgaggctatc atgcgcgtac tgctattttt acttctttcc cttttcatgt
      421 tgtcggcatt ttcggctgat aacctgttgc gctggcatga tgcgcagcat ttcacggtgc
      481 aagcctctat gccgcttaaa gccaaacgcg tatggaaact gtgcgcgctt tatcccagcc
      541 tgaaagattc atactggtta tcgttgaact atggtatgca ggaggctgct cgccgctacg
      601 gtgtggattt aaaagtgctg gaggcaggcg gctacagcca gttggctacc cagcaagcac
      661 aaatcgacca gtgtaaacag tggggcgcag aggccatttt gctcggtagt agcacgacgt
      721 catttcccga cctgcaaaag caggtagcaa atctgccggt gatcgaactg gtaaatgctc
      781 ttgatgatcc ccaggtgaaa agccgcgttg gtgtaccctg gtttcagatg ggctatcaac
      841 ctgggcgata tttggtgcaa tgggcgcacg gtaaaccact gaacgtgctg ctgatgcccg
      901 gacccgataa cgccgggggc agtaaggaga tggtcgaggg ttttcgcgca gccattgccg
      961 gaagcccggt gcgtattgtt gatattgcgc ttggtgataa cgatattgaa atccagcgca
     1021 acctgttgca ggagatgctg gaacgccacc cagaaatcga cgtcgttgcc ggaacggcca
     1081 ttgcggcaga ggcggcaatg ggtgaagggc gtaacctgaa aacgccgctt accgtggtgt
     1141 cgttttatct ttcacatcag gtgtatcgcg ggctgaagcg gggaagagtg attatggctg
     1201 ccagcgatca aatggtctgg cagggggaac tggcagttga gcaggccatc aggcaattac
     1261 aggggcaatc ggtgtctgat aatgtcagcc caccgatttt agttctgacg ccgaaaaatg
     1321 ccgaccgaga acatatccgc cgctcgctgt caccgggggg atttcgtccg gtctattttt
     1381 atcagcacac atcagcggct aagaaataac cttcaccatg ttgcgtcacc agtaaatccg
     1441 cgctgagttt atgacgtaaa cgacgaatta acacatcgac ggtgcgcagg tcagggtttt
     1501 ccacccgacg cgcagaaagc atacgcagca gacgctcacg gctgagaatt tcgcccggat
     1561 tagtcacaaa tgccaccaac atttcatact ctgcgcgggt cagtttaatc ggctcgccat
     1621 cccgctccag cgtatggcgc gacacattca ggcaataacc cgcaaagcga tagcagtagt
     1681 cctgagtgtg cggttgagct tgtcgcgcga ggtcgattcg ccagagcaga tttttcaccc
     1741 gtactaccag ttcgcgcagt tccagcggtt tggtgacgta atcgtctgcg cccatttcca
     1801 gcccaacaat acggtcaatc cgatcgctgc gtccggtaac cagaataatc cccaccgttg
     1861 agcgttctcg cagggcgcgg gttaacatca ggccattttc atcgggtaag ttgatatcca
     1921 gcagaattaa atctaccggc tgattctgca taatttcccg tagcccggca ccgctcgccg
     1981 taacggaaac ggtatacccc tcctgagtga agtaggattg taatcgcgcc tgggtaaccg
     2041 gcttatcttc aacaataaca atgtgatgtg gcatcagagg gttttactca ttctgttcat
     2101 atctgttcat atttgctcat taagatcgct tcactaaacc ataattctac aggggttatt
     2161 atgcggaaac tctggaacgc gctacgccga cccagtgctc gttggtcggt actggcgctg
     2221 gtcgcaattg ggattgtgat tggcattgcg ctgattgtat tgccacacgt tgggatcaaa
     2281 gtcaccagca caaccgaatt ttgtgtcagt tgccacagta tgcaaccggt gtatgaagaa
     2341 tataaacagt cggtgcattt ccagaacgcc tccggcgtgc gagctgaatg ccatgactgt
     2401 catatcccgc cggatattcc aggcatggtg aagcgcaaac tggaagcgag caacgacatt
     2461 taccagacct ttattgccca ctccattgat acacctgaaa aattcgaagc caaacgcgcg
     2521 gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca actcggcaac ctgtcgctcc
     2581 tgccataact acgatgcgat ggatcatgcc aggcagcatc ctgaagcggc acgtcagatg
     2641 aaggtggcag cgaaagataa tcaatcctgc atcgactgtc ataaaggtat tgcccaccag
     2701 ttaccggata tgagtagcgg cttccgtaag cagttcgatg agctgcgcgc cagtgctaat
     2761 gacagtggtg acacgctgta ctctattgat attaagccaa tttatgcggc gaaaggcgat
     2821 aaagaagcct ctggttctct gctgcctgct tcggaagtga aagtccttaa acgtgacggc
     2881 gactggctgc aaattgaaat taccggctgg acggaaagcg ccggacgtca gcgtgtactc
     2941 acccaattcc caggtaaacg catctttgtt gcctcgattc gtggtgatgt gcagcagcag
     3001 gtgaaaacgc tggagaaaac caccgttgcc gacaccaata ccgagtggag caagttgcag
     3061 gccaccgcgt ggatgaagaa aggcgacatg gtgaacgata tcaaaccgat ctgggcctat
     3121 gcggattcgt tgtacaacgg cacctgtaac cagtgccacg gcgcaccgga aatcgcccac
     3181 tttgacgcta acggttggat cggcacgctc aacggcatga ttggctttac cagtctcgat
     3241 aaacgtgaag aacgcacttt gttgaaatat ctgcaaatga atgcgtctga caccgcaggt
     3301 aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa taacgatctc tttcaggcat
     3361 cacgtcggcg ttttctggca caacttggcg gcttaaccgt cgccgggatg ctggggccgt
     3421 cattgttaac gccgcgccgt gcaactgcgg cgcaagcggc gactgaggct gtcatctcga
     3481 aagagggcat tcttaccggg tcgcactggg gggctatccg cgcgatggtg aaggatggtc
     3541 gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc gtcgaaaatg atcgccggat
     3601 tgccggatca cgtacacaac gcggcacgta ttcgttatcc gatggtacgc gtggactggc
     3661 tgcgtaagcg ccatctgagc gacacctccc agcgcggtga taaccgtttt gtgcgcgtga
     3721 gctgggatga agccctcgac atgttctatg aagaactgga acgcgtacag aaaactcacg
     3781 ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac ggggatgttc cataacgctt
     3841 cggggatgct ggcgaaagct attgccttgc atggtaatag cgttggtacg ggcggagatt
     3901 actctaccgg tgctgcgcag gtgatcctgc cgcgcgtagt cggttcaatg gaagtgtatg
     3961 aacagcaaac ctcctggccg ctggtattgc agaacagcaa aaccattgtg ctgtggggct
     4021 ccgatttgct gaaaaaccag caagcgaact ggtggtgccc ggatcacgat gtttatgaat
     4081 attacgagca gctaaaagcg aaagtcgccg ccggtgaagt tgaggtcatc agcatcgatc
     4141 cggttgtcac atccacccat gagtatctgg ggcgcgagca tgtgaagcac attgcggtta
     4201 acccgcaaac tgacgtgccg ctgcaactgg cgctggcaca tacgctgtac agtgaaaacc
     4261 tgtacgacaa aaacttcctc gctaactact gtgtgggttt tgagcagttc ctgccgtatc
     4321 tgctgggtga gaaagacggt cagccgaaag atgccgcatg ggctgaaaaa ctgaccggca
     4381 ttgatgccga aaccattcgt gggctggcgc ggcagatggc ggcgaacaga acgcaaatta
     4441 ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca gtgggcgtgg atgattgtgg
     4501 ttctggcggc gatgctgggg caaattggcc tgccaggtgg tggttttggc tggcactaca
     4561 acggcgcagg cacgccgggg cgtaaaggcg ttattctgag tggtttctcc ggctctacgt
     4621 cgattccgcc tgttcacgac aacagtgact acaaaggcta cagcagcact attccgattg
     4681 cccgttttat cgatgcgatc ctcgaaccgg ggaaagtgat caactggaac ggtaaatcgg
     4741 taaaactgcc gccgctgaaa atgtgtattt ttgccggaac taacccattc catcgccatc
     4801 agcagatcaa ccgcattatt gaaggctggc gcaagctgga aacggttatc gccatagata
     4861 accagtggac ctcaacctgc cgctttgccg atatcgtgct gcctgcgacc acgcagtttg
     4921 agcgtaacga tctcgaccag tacggcaacc actccaaccg tggcattatc gccatgaaac
     4981 aggtggtgcc gccgcagttc gaggcgcgca acgactttga tattttccgc gagctgtgcc
     5041 gtcgctttaa tcgtgaagaa gcctttaccg aagggctgga cgaaatgggc tggctgaaac
     5101 gcatctggca ggaaggtgta caacaaggca aaggacgcgg cgttcatctg ccagcgtttg
     5161 atgacttctg gaataacaaa gagtacgtcg agtttgacca tccgcagatg tttgttcgcc
     5221 accaggcatt ccgcgaagat ccggatctcg aaccgctggg cacgccgagt ggcctgattg
     5281 agatctactc gaaaaccatc gccgatatga actacgacga ttgtcagggg catccgatgt
     5341 ggtttgagaa aatcgaacgc tcccacggtg ggcccggctc gcagaagtat ccgttgcatc
     5401 tgcaatctgt gcatccggat ttccgacttc actcgcagct atgtgagtcg gaaacgctgc
     5461 gtcagcaata tacggtagcg ggtaaagagc cagtgttcat taacccgcag gatgccagcg
     5521 cgcgcggtat tcgtaacggt gatgtggtac gcgtctttaa cgctcgcggt caggtgttgg
     5581 caggagcagt agtttctgac cgctatgcac ccggcgtggc gcgaattcac gaaggggcat
     5641 ggtacgatcc agataaaggc ggcgagcctg gtgcgctgtg caaatacggt aaccccaacg
     5701 tgctgaccat cgacatcggg acttcgcagc tggcgcaggc ggccagtgcg cacactacgc
     5761 tggtggaaat tgagaagtac aatggaacag tggagcaggt gacggcgttt aacggccccg
     5821 tggagatggt ggcgcagtgc gaatatgttc ccgcgtcgca ggtgaaatca tgaccacgct
     5881 gacagcacaa cagattgcct gtgtttacgc ctggctggcg cagttgttct cccgtgaact
     5941 ggacgatgaa caactgacgc aaatcgccag tgcgcagatg gctgaatggt tttcgttgct
     6001 gaaaagcgaa ccgccgctca ctgcggcggt gaatgatctg gaaaacagta ttgccac
//
LOCUS       DAEEGA010000162         6033 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEEGA010000162 DAEEGA010000000
VERSION     DAEEGA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.163"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            91..867
                     /gene="exuR"
                     /locus_tag="KLH46_003870"
     CDS             91..867
                     /gene="exuR"
                     /locus_tag="KLH46_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBE0380371.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1212..1874
                     /locus_tag="KLH46_003871"
     CDS             1212..1874
                     /locus_tag="KLH46_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0380372.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1878..2261
                     /gene="mzrA"
                     /locus_tag="KLH46_003872"
     CDS             1878..2261
                     /gene="mzrA"
                     /locus_tag="KLH46_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBE0380373.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2408..2762
                     /locus_tag="KLH46_003873"
                     /pseudo
     CDS             2408..2762
                     /locus_tag="KLH46_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2800..3105
                     /locus_tag="KLH46_003874"
     CDS             2800..3105
                     /locus_tag="KLH46_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBE0380374.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3108..3512
                     /locus_tag="KLH46_003875"
     CDS             3108..3512
                     /locus_tag="KLH46_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBE0380375.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3502..3801
                     /locus_tag="KLH46_003876"
     CDS             3502..3801
                     /locus_tag="KLH46_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBE0380376.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3987..4283
                     /locus_tag="KLH46_003877"
     CDS             3987..4283
                     /locus_tag="KLH46_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBE0380377.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4353..5339
                     /gene="yqjG"
                     /locus_tag="KLH46_003878"
     CDS             4353..5339
                     /gene="yqjG"
                     /locus_tag="KLH46_003878"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBE0380378.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5632..6000
                     /locus_tag="KLH46_003879"
     CDS             5632..6000
                     /locus_tag="KLH46_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBE0380379.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
BASE COUNT         1526 a         1471 c         1519 g         1517 t
ORIGIN      
        1 ttcaatcatc aaatttatat atgaccaatg gcatacccat gaataaagtg gtatgataac
       61 ttcgtccgca ttccactccg ggagacgcat atggaaatca ctgaaccacg ccgtttgtat
      121 caacaacttg ctgctgacct gaaagagcgc attgaacagg gcgtctatct ggtgggtgat
      181 aaactgcctg ccgaacgctt tattgccgat gaaaagaacg tcagccgaac ggtagttcgt
      241 gaagccatca tcatgctgga agttgaaggc tatgtggagg tgcgtaaagg ttcaggtatt
      301 catgtggttt ccaaccagcc gcgtcatcag caggcggctg acaataatat ggaattcgcc
      361 aattacggtc cgtttgagtt gcttcaggct cgccagctca tcgaaagtaa tattgccgag
      421 ttcgcggcaa ctcaggtaac gaaacaagac atcatgaaac tgatggccat tcaggaacag
      481 gcgcgcggcg aacaatgctt ccgtgattcc gagtgggatt tacagttcca cattcaggta
      541 gccctggcga cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg gacccagcgt
      601 agccataacc cgtactggaa aaaactacac gaacacattg attcccgtac cgttgataac
      661 tggtgtgatg accacgatca aattctcaag gcgttaattc gcaaagatcc tcatgctgct
      721 aagctggcaa tgtggcagca tctggaaaac accaaaatca tgttatttaa cgaaaccagc
      781 gacgatttcg agttcaatgc cgaccgctat ctgttcgccg aaaacccggt agtgcatctc
      841 gatacggcca ccagcggcag caaatgaaat tcctgtccga caggcgctgc aatgcgcctg
      901 tttgtctcgt cggtaagcga aaagtataaa gtgtcagcct gtgtaaatcc tctcgccacc
      961 ctcccctgca atcagcaaaa tcagactcca cggacatgta attttgataa cgaacaacgt
     1021 tgacctttgt tacaattaga ttcaatttga atttatgctt ttgaatgctt tcttatctca
     1081 cgttttaaca gggaatagtt caggctgtgt tgatgtatca aacccgcaga acataccaaa
     1141 acagcaataa cattgcggta gtgcatcttt taaaaccagc gtggcgttaa ccgattcacc
     1201 aggaataatg aatggaactt ttgacccaat tgctgcaagc cctgtgggcg caggattttg
     1261 aaaccctggc caatccatcg atgattggca tgttgtattt tgtcttgttt gtaattttgt
     1321 tccttgaaaa tggcttgctt ccggcggcct ttttaccggg cgacagttta ctggtattgg
     1381 tcggcgtgtt gattgcgaaa ggcgcgatgg gttacccgca aacgattctg ctgctgaccg
     1441 ttgccgccag cctcggttgc tgggtcagct atattcaggg gcgatggctg ggcaataccc
     1501 gcaccgtaca aaactggcta tctcatttac ccgcgcatta tcatcaacgc gcacaccatc
     1561 tttttcataa acacggttta tcggcgctgt taattggtcg ctttattgca tttgtcagaa
     1621 cactgctgcc gacgattgcc gggttatcag ggctgaataa cgcgcgcttt cagttcttca
     1681 actggatgag cggtctgctg tgggtattga tcctgacaac tctgggttac atgctcggca
     1741 aaacgccggt atttttaaag tacgaggacc agctgatgtc atgcctgatg ctgctcccgg
     1801 tggtgctgct ggtgtttggc ctggcaggtt ctctggtcgt gttatggaaa aagaaatatg
     1861 gaaatcgggg gtaagggatg caaatacctc gcatgtcgct tcgccagcta gcctggtccg
     1921 gcactgtttt acttctggtc ggcacgctgt tgctggcctg gtccgcggtt cgccagcaag
     1981 agtctacgct ggcgattcgt gccgttcatc aaggcacaac gatgccagac ggtttttcga
     2041 tctggcatca ccttgacgct catggcattc ctttcaaaag tatcaccccc aaaaacgaca
     2101 ctctgttaat tacatttgac tccagcgacc agagcgccgc cgcaaaagcg gtcctcgaca
     2161 gaacattgcc ccatggctac atcattgcgc agcaggacaa taacagtcag gctatgcagt
     2221 ggctgacccg gttacgggat aactctcatc gcttcggata acttcctgtt atccgaaaca
     2281 tatcactcac tttggtgatt tcaccgtaac tgtctatgat taatgaggcg gtggttaaca
     2341 ctaccgcttc gttgtctgga caatcgttcc tttgtaatag gtccgaatca caatggaagg
     2401 ttcaagaatg aaataccgca tcgctttagc tgtttctctc tttgctctta gtgccggtag
     2461 ttatgccact accctgtgtc aggaaaagga gcaaaatatc cttaaggaga tcagctatgc
     2521 cgaaaaacac caaaaccaga atcgtattga cggtctgaat aaagccctga gtgaagtccg
     2581 ggccaactgt tcagatagcc agctgcgtgc cgatcatcac aagaaaatcg caaagcagaa
     2641 agatgaggtg gcggaacgcc agcaagattt agccgaggcg aagcaaaaag gcgatgccga
     2701 tagaacggaa actggcagaa gcgcaggaag agctgaaaaa gctggaagcg cgcgactact
     2761 aactcacaat agtcactact tactcacctg gagaaaacta tgtcgaaaga acacactacg
     2821 gaacatctgc gtgctgagtt gaaatccctt tccgatacgc tggaagaggt gcttagctca
     2881 tctggcgaga agtcgaaaga agagttgagt aagattcgta gcaaagcgga gcaggcactg
     2941 aaacagagcc gttatcgcct gggtgaaacc ggtgatgcca tcgccaaaca aacccgtgtc
     3001 gcggcggcgc gtgctgatga gtatgtgcgt gaaaatccgt ggaccggcgt gggaattggc
     3061 gctgcaatcg gtgtagtgct cggcgttctg ctgtcgcgtc gttaattatg gcggacactc
     3121 atcacgcgca agggcccggt aaaagcgttc tgggcatcgg gcagcgaatt gtttccatca
     3181 tggttgaaat ggtggaaaca cgtctgcggc tggcggtggt ggagctggaa gaggaaaaag
     3241 ctaatctctt tcaactttta ctgatgctgg gcctgacgat gcttttcgct gcatttggtc
     3301 ttatgagcct gatggtgcta attatttggg cggttgatcc gcaatatcgc ctgaatgcga
     3361 tgattgccac caccgtggtg ttgctgctac tggcactgat tggcggtatc tggacgttac
     3421 gtaaatcgcg taaatccacc ttgctgcgcc atacacgcca tgagctggca aacgatcggc
     3481 aactgctcga ggaggagtcc cgtgagcagt aaagtcgaac gtgaacgacg taaggcgcaa
     3541 ctgcttagcc agatccagca acaacggctg gatctttccg ccagtcgtcg tgaatggctg
     3601 gaggcaacag gcgcttacga tcgtcgctgg aatatgctgc taagtctgcg ctcctgggcg
     3661 ctggttggca gtagcgtgat ggcgatctgg acgattcgcc atcctaatat gctggtccgc
     3721 tgggccagac gcggttttgg cgtatggagc gcctggcgtc tggttaaaac gaccctcaag
     3781 cagcaacagc ttcgcggtta acttccctct ggccggagca attccggcct tatccctcaa
     3841 attttttgaa tatttttgac agttttcctt gctaacaatc atcattcacc acgtttatga
     3901 ttctctccat cgacagcaac gacgctaata gcgcgccctt gcacaaaaaa acaatcagca
     3961 gcctgagtgg cgcagtggag agtatgatga aaaaattaga agatgttggt gtactggtag
     4021 cgcacatttt aatgccgatt ctgtttatta ccgcaggctg gggaaaaatc accgcttact
     4081 ctggtaccca acaatatatg gaagcaatgg gtgtcccggg ttttatgctg ctgacggcat
     4141 ttttatttca cagcaacttt gctgaaggcg tcaactcgct gatgttcatg aaaaacctga
     4201 caatttctgg cggattcctg ctgctggcaa ttaccggtcc gggcgcgtat agcatcgacc
     4261 gcctgctgaa taaaaagtgg taagcacgcg ctatacttaa cgataaaaaa cgaggaggaa
     4321 gctcctcgtt tttgctattg gaggagagaa aaatgggtca actgattgac ggcgtctggc
     4381 atgacacctg gtacgatacc aaatctaccg gcggtaaatt tcaacgttca gcttccgcat
     4441 ttcgtaactg gctcactgcc gatggcgcac ctggccccac tggcacaggc ggttttatcg
     4501 cagagaaaga tcgttatcat ctctatgttt cactcgcctg cccgtgggcg caccgcacgc
     4561 tgatcatgcg caaactcaaa ggactggaac cgtttatttc cgtttccgta gtgaacccgc
     4621 tgatgctgga aaacggctgg acctttgatg acagttttcc gggagcaacc ggagatacgc
     4681 tctatcaaca tgaatttctg tatcagcttt atctccacgc cgatccacac tacagcggac
     4741 gagttactgt tcccgtgctg tgggacaaaa agaaccacac catcgtcagc aacgaatcag
     4801 cagaaatcat ccgcatgttc aataccgcgt ttgatgcgct gggcgcaaaa gcgggtgatt
     4861 actacccacc agccctgcaa acgaaaattg acgaacttaa cggctggatt tatgacaccg
     4921 ttaacaacgg cgtgtataaa gctggttttg ccaccagcca gcaagcctac gacgaggcgg
     4981 tggcgaaagt gtttgaatcg ctggcgcgac tggagcagat tttaggccag catcgttacc
     5041 tgaccggcaa ccagctaacc gaagccgata ttcgcctgtg gactacgctg gtgcgttttg
     5101 atccagtgta tgtgacccac ttcaagtgtg ataagcaccg catcagcgat tacctgaatc
     5161 tgtatggctt cctgcgcgat atctaccaga tgccggggat cgccgaaact gtcaatttcg
     5221 atcacatccg taatcattac ttccgcagcc ataagaccat caaccctacg gggattattt
     5281 caattggtcc gtggcaggat ctcgatgaac cgcatggacg agatgttcgc ttcggttaaa
     5341 aaaaggcacc ttacgaggtg cctctgataa ttagctaagt gcatgttgtt catgtcggat
     5401 gcggcgtgaa cgccttatca aacctacaaa gcgtttatat tcaatatgtt acaatgaccg
     5461 cgtaggccag ataagcgtag cgcatctggc gattttgcgc ttgccatcag tcttagcgcg
     5521 ccttcacggc actttattaa ttccttgaac gaatatttac tgccatttca ttcacgtcta
     5581 ttcttaattt gctgctttaa aaacaagtga ttgagcaaaa ttgaggcaaa aatggactgg
     5641 tatctgaaag tactaaaaaa ttatgtcggt ttccggggtc gtgcgcggcg caaagagtac
     5701 tggatgttta ttctggtcaa catcatcttt acgttcgtgc tggggctgct ggataaaatg
     5761 ttaggctggc aacgcgctgg tggcgaaggc atcctgacga taatctacgg tattctggtg
     5821 tttttaccgt ggtgggcggt tcagttccgc cgcctgcacg acaccgaccg ctcggcgtgg
     5881 tgggcactgc tattcttaat cccgtttatc ggctggctta ttatcatcgt ctttaactgc
     5941 caggcgggta cgccaggcga aaaccgcttt ggaccagatc caaaactgga acaagaataa
     6001 tgattaagca ccttgtaaaa ggtgcttcaa att
//
LOCUS       DAEEGA010000163         5983 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEEGA010000163 DAEEGA010000000
VERSION     DAEEGA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.164"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(1..393)
                     /locus_tag="KLH46_003880"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="KLH46_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="KLH46_003881"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="KLH46_003881"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HBE0380380.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGIIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="KLH46_003882"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="KLH46_003882"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HBE0380381.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..>5983
                     /locus_tag="KLH46_003883"
     CDS             5797..>5983
                     /locus_tag="KLH46_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0380382.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1839 a         1354 c         1019 g         1771 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattatac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg tagattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg agg
//
LOCUS       DAEEGA010000164         5983 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEEGA010000164 DAEEGA010000000
VERSION     DAEEGA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.165"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..262)
                     /locus_tag="KLH46_003884"
     CDS             complement(<1..262)
                     /locus_tag="KLH46_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBE0380383.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFV"
     gene            483..911
                     /gene="uspG"
                     /locus_tag="KLH46_003885"
     CDS             483..911
                     /gene="uspG"
                     /locus_tag="KLH46_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBE0380384.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1032..2597)
                     /gene="ahpF"
                     /locus_tag="KLH46_003886"
     CDS             complement(1032..2597)
                     /gene="ahpF"
                     /locus_tag="KLH46_003886"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBE0380385.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2842..3405)
                     /gene="ahpC"
                     /locus_tag="KLH46_003887"
     CDS             complement(2842..3405)
                     /gene="ahpC"
                     /locus_tag="KLH46_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBE0380386.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            3777..4523
                     /gene="dsbG"
                     /locus_tag="KLH46_003888"
     CDS             3777..4523
                     /gene="dsbG"
                     /locus_tag="KLH46_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBE0380387.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            4733..5635
                     /locus_tag="KLH46_003889"
     CDS             4733..5635
                     /locus_tag="KLH46_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0380388.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            5782..5983
                     /locus_tag="KLH46_003890"
                     /pseudo
     CDS             5782..5983
                     /locus_tag="KLH46_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         1605 a         1441 c         1348 g         1589 t
ORIGIN      
        1 tgacgaacgg aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc ttcccggttt
       61 caacgacttc ccccataaat tcatgaccaa aaatatcgcc atgtttaacc tggggtattt
      121 tgcctcgata aagatggagg tcagagccac agatcgccgt taccgtaata cgcagaataa
      181 tatcatctgc ctgttcaatg cccggatcgg gaacattttc tacctgaacg tgatgtgggc
      241 cgtgataagt caatgctttc atgcaacctc cagagacagt tcaaaaaaga gtcagtcttt
      301 aagggtagct atcacagcgg gaaaacgggc ggtaccgcag gttttgggag aattcctagt
      361 gttcagtgag aatatattaa tcacagaaac cgttcgtaag gaaaaagaac cagacaaata
      421 tcagggataa atattataat catggagaat gatttgttca tgattaacag ggagaaaggg
      481 ttatgtataa ggcaatcatt atgccagttg atgtatttga aatggaattg agcgacaaag
      541 ctgttcgcca cgctgaattc ctcgcccagg atgacggagt tattcatcta cttcacgtac
      601 tacccgggtc agccagcctg agcctgcacc gttttgccgc tgatgtgcgt cgttttgaag
      661 agcatctgca acatgaagca gaagaacgtc tgcaaacgat ggtcagccac ttcaccatcg
      721 atccttcccg cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa gtcaatgagt
      781 tggcagaaga actgggggct gatgttgtag ttatcggttc tcgcaaccca tcgatttcga
      841 cccatctgtt aggttctaac gcctcgagcg tgatccgcca cgccaatctg cctgtactgg
      901 tcgttcgcta acagataaaa gtatttatgc cgccctgcct ggaacatccc ggcagggctt
      961 tttaatgcca taaaaaagcc gccaggtttg accccggcgg ctaagcaatt gcaggtgaat
     1021 cttacttctt cttatgcagt tttggtgcga atcaggtagt caaaagcact cagagaggct
     1081 ttggcacctt cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt acagtcaccc
     1141 gctgcgaaca cgcctttgac gttggtttca catttcgcat caatgataat ctcgcccatg
     1201 cggttacgtt cgactgcgcc ttccagccag ttggtgttcg gcagcagacc aatctggaca
     1261 aaaataccgg ccagttcgat gttgtgaata ttgccgctga cacgatctcg atattccaga
     1321 ccaacgactt tgctgccgtc gcctttcact tccgtggttt gcgcattcag aataatgtcg
     1381 acgtttttca ggctgcgcag tttgtcctgc agaacctggt cggctttcat ttctggcgca
     1441 aattccagca gcgttacgtg ctcaacgata cccgccaggt cgattgccgc ttccacgccg
     1501 gagttaccgc cgccgataac cgctacgcgt ttgcctttaa acagcggtcc gtcgcagtgc
     1561 gggcagtagg tcacgccttt agtgcgatac tgatcttcgc ccggaacgtt catgttgcgc
     1621 cattttgcac ccgtcgccac gataatgctg cgtgctttca gtaccgcgcc agaagctgtt
     1681 tcaatctggt gtagaccacc ttcaaccgct gccgggatca gtttgctggc gctctggctg
     1741 tcgatcacat caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc caacttctgc
     1801 ccttcagtct tcggtacaga aatgtagttt tcgatatcaa cggtatcgag gatctgacca
     1861 ccaaaacgtt cgcccatcag accggtacgg atgcctttac gtgcggagta aattgctgcc
     1921 gctgcacccg ccgggccgga accaacgatt aatacgtcat aagcatcacg cttgttcagc
     1981 tcttctgccg cacgtttttc cgcgccagta tcaattttgg caacgatttc agtcaacgtc
     2041 atgcggccct gaccaaactc tttcccgttt acgaacactg ccggaacgcc catcacgttg
     2101 cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt gatgcgcggg
     2161 ttcaatacgc tcatcaggtt cagcgcctgc accacgtccg ggcagttatg gcaagagagc
     2221 gagtaatagg tttcgaattc aaaatcaccg tcaatatggc gaatctgctc cagcagagac
     2281 tgcgcttctt tcgacggatg accaccggtc cacagcaacg ccagtaccag cgaggtgaac
     2341 tcgtggccca gcggggagcc tgcaaaacgt ggtccctggt tggaacctgg gttggtgatc
     2401 aggaaagacg gcttacgcac tggcaagctg ttatcttctt taaaggtgac tttgtctgac
     2461 agttctgcga tttcagccaa cagttccttg atttctgccg atttagcgct gtcatccagc
     2521 gtggcaatta actcaacagg cttggtcaat ttctcaaggt aagccttgag ttgagttttc
     2581 atatttgtgt cgagcatgtt tatctcctgg gctttaaaca tcatcatgca agcggcctct
     2641 gcggctacct ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt gccgcagcac
     2701 ccgaagaatt aggtgaattt ccgaagtatt tcgcgtcaga gcaaggcgac tagcctgtga
     2761 atccccggga gcttacacaa gtaagtgacc ggggtgagcg ggcgacgcca acgccgctat
     2821 ggcgtgaaag acgaaggaaa tttagatttt accaaccagg tccagagacg gagccagagt
     2881 tgcttcacct tctttccatt tagccgggca aacttcacct gggtgagaag ctacgtactg
     2941 agctgctttg attttacgca gcagatcaga cgcgtcacgg ccaatgcctt cagcggtaac
     3001 ttcgattgcc tggatgatac cctgcgggtc aacaacgaag gtcgcacggt cagccagacc
     3061 ttcatcttca cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt cgccgatcat
     3121 cgcatatttg attttagcga tggtttcaga gctgctgtgc catgctttgt gggtgaagtg
     3181 agtatcggta gatactgcgt atacgtctac gcccagtttc tgcagttctt cgtagtggtc
     3241 agcaacgtca cccagttcgg tcgggcatac gaaagtaaag tcagccgggt agaagaagaa
     3301 gacgctccag cggccttcgg tatctttttc ggtgatttcg atgaattcgc cgtttttgaa
     3361 tgcctggttt ttaaaaggtt taattttggt gttaatcaag gacatctata cttcctccgt
     3421 gttttcgatg agatgtaagg taaccaattt ttgccgattc ggctaatgcg tttccattat
     3481 caaatcgata agttttacct tacaaccttc gtaagacaac tttgtgaact ttgcaccgcc
     3541 agcggcgata aaaagtaaaa tggccccctt tccaggtgac cgtgtaatct gaactaccct
     3601 ttggtatctt cagcgcctgc ctgcaggctg aggtgttgcc cttacaaatg caacaacgac
     3661 atggattaca acaccctcat aaacaaaggg caatcacctg atctaagctc ttacctatga
     3721 cagtgatagg ttatgccttt tactcgactt ttgcactgac tgaaaagaac aaattaatgt
     3781 taaaaaagat acttttactg gctctgcttc ctgcaatcgc cttcgcagag gaacttcctt
     3841 ccccagtaaa agcgattgaa aaacagggca ttacaatcat caaaacgttc gatgcccccg
     3901 gaggaatgaa aggttatctc ggaaagtatc aggatatggg cgtcaccatc tacctgactc
     3961 ccgatggtaa gcacgctatc tctggttaca tgtacaacga gaaaggtgaa aacctgagta
     4021 acacacttat cgaaaaagaa atttacgcac cagccggacg cgaaatgtgg caacggatgg
     4081 aacaatccca ctggctcctc gacggtaaaa aagatgcgcc ggtcattgtc tacgtcttct
     4141 ccgatccgtt ctgcccatat tgtaaacagt tctggcagca ggcgcgcccg tgggtagatt
     4201 ctggcaaagt gcaattaaga acattgttgg ttggggttat caagccagaa agcccggcga
     4261 cagcagcggc aattcttgcc tccaaagatc ccgcaaaaac ctggcaacaa tatgaagcct
     4321 ctggttgcaa gcttaagcta aacgtgcctg caaacgtaag tacagagcaa atgaaagtgt
     4381 taagtgacaa tgagaaactg atggacgatc tgggggcaaa tgtcacgccg gctatctatt
     4441 acatgagtaa ggaaaatacg ctgcaacagg ccgtggggtt gcccgatcag aaaacgctta
     4501 atatcattat gggaaataaa taaaaggtaa ttcatggatg gttgaactat atccctgctt
     4561 ttcaaccatc ctaaaaaagc atgaatttct cgctctaccc taagtggctg tgctatttta
     4621 gaaaaactta atcctgagtt aagcaaaaaa acataatcaa taaaaataca tacgcaaaaa
     4681 acattgacta agtgaatata ccatggaaga aaaatataaa ccggagtagt gtatggccaa
     4741 tctctacgac ttgaaaaagt tcgacttaaa tcttcttgtc atatttgagt gtatttacca
     4801 gcatttaagc attagcaaag ctgctgaatc actatatatc accccatcag ccgtgagcca
     4861 gtcgttacaa cgcttacgtg cgcaatttaa tgacccttta ttcattcgct caggaaaagg
     4921 tatcgcgccg acgacaacag ggttgaatct gcatcaccat cttgagaaaa acctcaaagg
     4981 ccttgaacaa acaattaata tagtcaataa gtccgaacta aagaaaaact ttattatcta
     5041 cggccctcag ctcatttctt gctctaataa tagtatgctg atccgctgcc tacggcaaga
     5101 tgcctctgtc gagattgagt gccatgatat cctcatgtca gcagagaatg ctgaagaact
     5161 gttggttcac cgcaaagccg atctggtgat cacccaaatg ccagtgatca gccgttccgt
     5221 catttgcatg ccattgcata caattcgcaa tactctaatt tgtagtaata agcatccgcg
     5281 aattactgac aatagcactt atgaacaaat tatggcagaa gagttcaccc aattaatagc
     5341 caaatcagca ggagttgatg atattcaaat ggaaatagac gagaagttta tgaatcgcaa
     5401 aatttcattt cgtggttcct ctttgctgac aataattaat agcattgcag ttacagattt
     5461 actgggaata gtcccctatg aactttataa ctaccaccgt gactttctta atttaaaaga
     5521 gataaaacta gaacatccgt taccttcaat aaagctttac atttcctata ataaatcttc
     5581 tttaaataat ttggtgttct cccgatttat tgaccgtcta aatgagagtt tttgatatat
     5641 aactatagac agctactata actttcatct atttattcac agcgcgaatg aaaaataaaa
     5701 cactctgctt tacagtgttt actttataat taacagtctt atctcactgt tcaccctgta
     5761 acgttcagag agcgtcttca gatgtctatt tataaaattc ctcttccgct caatattctg
     5821 gaagctgcac gagagcgtat tacctagaca ctcaacaccc taccccgcgt ttgtgtctca
     5881 ttttctggcg gcaaagactc tggtttaatg ctgcatctga cagccgaact tgcccgacaa
     5941 atgggcaaaa aaatctgcgt tttgtttatc aactgggagg cgc
//
LOCUS       DAEEGA010000165         5918 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEEGA010000165 DAEEGA010000000
VERSION     DAEEGA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.166"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..116
                     /locus_tag="KLH46_003891"
     CDS             <1..116
                     /locus_tag="KLH46_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0380389.1"
                     /translation="NLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(128..1075)
                     /gene="hprS"
                     /locus_tag="KLH46_003892"
                     /pseudo
     CDS             complement(128..1075)
                     /gene="hprS"
                     /locus_tag="KLH46_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
     gene            complement(1075..1746)
                     /gene="hprR"
                     /locus_tag="KLH46_003893"
     CDS             complement(1075..1746)
                     /gene="hprR"
                     /locus_tag="KLH46_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBE0380390.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1879..2292
                     /gene="hiuH"
                     /locus_tag="KLH46_003894"
     CDS             1879..2292
                     /gene="hiuH"
                     /locus_tag="KLH46_003894"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBE0380391.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2401..3405
                     /gene="msrP"
                     /locus_tag="KLH46_003895"
                     /pseudo
     CDS             2401..3405
                     /gene="msrP"
                     /locus_tag="KLH46_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3406..4041
                     /gene="msrQ"
                     /locus_tag="KLH46_003896"
     CDS             3406..4041
                     /gene="msrQ"
                     /locus_tag="KLH46_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBE0380392.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4299..4949
                     /gene="zinT"
                     /locus_tag="KLH46_003897"
     CDS             4299..4949
                     /gene="zinT"
                     /locus_tag="KLH46_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBE0380393.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1683 a         1334 c         1229 g         1672 t
ORIGIN      
        1 tgaatctgag acaacatctg gcaaggctgg gacggaagtc actgtcgttc tcaaaatcgg
       61 tggagctgca tgacaaggtc atcgggcatt atctgaacat aaaacactat cagtaagttg
      121 gagtcattac ctccttattg agagaaagcg aatccagttt caccaaaaca ttgtttttgt
      181 ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc agctcttcaa
      241 tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg agcgtaacct
      301 gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga tcgtcagcaa
      361 attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc aacgcctgcc
      421 caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg ctatcgttat
      481 aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc gtcctgatta
      541 acaacggact taatactgag cacagtataa tggcgacaat gcaaattata atgctattaa
      601 ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca gtaaccgtta
      661 atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta tcattaataa
      721 tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta tttaacatgt
      781 catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga atgatcaaaa
      841 tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct ggatttaccc
      901 catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta tcatcgcgcc
      961 atttcaactc acttgccagg ccattataga gagtccatac aattccagcg ccagcaacag
     1021 actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt ttcattattt
     1081 ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct tttcaggaaa
     1141 aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat tggtatcact
     1201 atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa tctcgccagc
     1261 tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa tactgatatt
     1321 gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt ctaatgttga
     1381 attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact cagaaaatga
     1441 aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc tgtcatcgac
     1501 agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta acgtttgtaa
     1561 gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg cataatcatc
     1621 cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga cataacccgc
     1681 ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa taagtagaat
     1741 cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt tcattacaaa
     1801 attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa aactcattca
     1861 catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc aacggcagca
     1921 ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt gcacattttg
     1981 aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa gaaagcggat
     2041 aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat taaggcactg
     2101 tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac cggggactat
     2161 ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt tcatattaat
     2221 aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta ttcaacctat
     2281 cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag ggaggtgact
     2341 ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt gtgatggctt
     2401 atgaaaaaga agcaattttt aaaagaacca gatgttacgg ccgagtcggt attctttatg
     2461 aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc tttgcctcac
     2521 gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc cgcccccgcc
     2581 ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc actgacgcca
     2641 gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa agacgatccc
     2701 gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag cggcgaagtg
     2761 gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct ggaagagcgt
     2821 atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat tggttttccg
     2881 ctacacaaat tactggcgct tgccgaaccc accagcaatg cgaagtatgt cgctttcgaa
     2941 acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg cggcgggctg
     3001 aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct cacactgatg
     3061 accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt gcgactgatt
     3121 gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa gctgacccgc
     3181 gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt ttacgccaac
     3241 gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt tattggttca
     3301 ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta cgccgaccag
     3361 gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg tctgacagca
     3421 aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc gtttctctgg
     3481 ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga tattcagcat
     3541 tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac acctctggca
     3601 cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt atggtgcttt
     3661 gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt gaataatctg
     3721 gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat tatcagctgg
     3781 gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa actcggcaaa
     3841 cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc aatacattat
     3901 ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgctgggct ggctgtactg
     3961 cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg caagcaggcc
     4021 cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat cccggccgga
     4081 tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt tcgtttgtca
     4141 tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca atacacctga
     4201 gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt acaatataac
     4261 attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt cttcataaac
     4321 tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat ggtcatcatt
     4381 ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt tttgatgata
     4441 ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc gtttaccctt
     4501 tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca gataaaacca
     4561 aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat atcgaaatga
     4621 ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca tcctgtaaat
     4681 atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc gttcgttact
     4741 tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt agcgatcata
     4801 taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat tcccagcaat
     4861 ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg agtagcgaag
     4921 aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt tatttaacag
     4981 aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga caaactcaga
     5041 cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac tgagaaaaag
     5101 gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt tccaggagca
     5161 agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt ttaaaccttt
     5221 tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa caactcttgg
     5281 aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt ttggctcaat
     5341 tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc agtttcatag
     5401 gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa ctgtagattt
     5461 tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc acagtatcga
     5521 acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct gtttaggaaa
     5581 agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag ggggatttga
     5641 acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga gtcgaaaaat
     5701 caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga aaaatcaatg
     5761 aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac ctgttttcag
     5821 gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct cagcttattc
     5881 cgttttgcct gtgtgttgaa taattccact gcagttat
//
LOCUS       DAEEGA010000166         5867 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEEGA010000166 DAEEGA010000000
VERSION     DAEEGA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.167"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            28..243
                     /locus_tag="KLH46_003898"
     CDS             28..243
                     /locus_tag="KLH46_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBE0380394.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            236..622
                     /locus_tag="KLH46_003899"
     CDS             236..622
                     /locus_tag="KLH46_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HBE0380395.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            630..1316
                     /locus_tag="KLH46_003900"
     CDS             630..1316
                     /locus_tag="KLH46_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBE0380396.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1294..1917)
                     /gene="tetR(B)"
                     /locus_tag="KLH46_003901"
     CDS             complement(1294..1917)
                     /gene="tetR(B)"
                     /locus_tag="KLH46_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HBE0380397.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1999..3204
                     /gene="tet(B)"
                     /locus_tag="KLH46_003902"
     CDS             1999..3204
                     /gene="tet(B)"
                     /locus_tag="KLH46_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBE0380398.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3317..3910)
                     /gene="tetC"
                     /locus_tag="KLH46_003903"
     CDS             complement(3317..3910)
                     /gene="tetC"
                     /locus_tag="KLH46_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HBE0380399.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4043..4414
                     /locus_tag="KLH46_003904"
     CDS             4043..4414
                     /locus_tag="KLH46_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0380400.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4424..5587)
                     /locus_tag="KLH46_003905"
                     /pseudo
     CDS             complement(4424..5587)
                     /locus_tag="KLH46_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
     gene            complement(5602..>5867)
                     /locus_tag="KLH46_003906"
     CDS             complement(5602..>5867)
                     /locus_tag="KLH46_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0380401.1"
                     /translation="ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQR
                     IERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         1606 a         1110 c         1282 g         1869 t
ORIGIN      
        1 ccactattaa gtcatgtagc tggttttatt tcaattgaac gttttcaaag tctagctaca
       61 gaaggaaaaa tgttatcgct atcttggtgg gaaaacgaat acgcagttct gcaatggaaa
      121 aatcatgttt tacatgcgaa agctcaacaa gaagggcgag agtcaatatt tgatttttac
      181 aaaattagta ttgctcatat tactcgcgaa tattcattta aaaaggacaa ggataatgtt
      241 tgatgttcac gttgttttag ataatcaaat aggacaatta gcattactag gaaaaacatt
      301 aggtaataaa ggtattggat tggaaggggg agggatattt acggttggtg atgaatgcca
      361 tgctcatttt cttgttgaac aaggaaagga agctaaaata gcgctagagc aagctggact
      421 gttagtactt gcgatccgga caccattaat tcgtaagtta aaacaggaaa aaccggggga
      481 acttggcgaa atagcacgag tattggcgga gaataacatt aatattttag tgcaatacag
      541 tgaccatgct aaccaactga tattaataac ggacaatgat agtatggctg catctgttac
      601 gctcccttgg gcaataaagt gaacttgcga tggctaattt aatacgaaaa gaggttacct
      661 ttgagtcctc aatagccgcg ataggggcgg ctatgtctga catttcacga gttaaaatac
      721 tcagtgcttt gatggatggg cgagcttgga cggccactga gctaagttct gtggcgaata
      781 tatcagcttc aacggcgagc agtcatttat ctaaattatt agattgccag ctaatcacag
      841 tagtagctca aggcaagcat cgttattttc ggctagcagg aaaagatatt gctgaattga
      901 tggaaagtat gatggggatc tccttaaacc atggcgtaca tgccaaagtt tccacgccag
      961 tgcatttacg aaaagcacgt acttgctatg atcatttagc tggcgaagtt gccgttaaga
     1021 tctatgattc cctttgtcaa cagcaatgga tcactgaaaa tggttcaatg atcacattaa
     1081 gtggtattca atattttcat gaaatgggaa ttgacgttcc ttccaaacat tcacgtaaaa
     1141 tctgttgtgc gtgtttagat tggagtgaac gccgtttcca tttaggtggg tacgttggag
     1201 ccgcattatt ttcgctttat gaatctaaag ggtggttaac tcgacatctt ggttaccgtg
     1261 aagttaccat cacggaaaaa ggttatgctg cttttaagac ccactttcac atttaagttg
     1321 tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct ctgcaccttg
     1381 gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag tagtaggtgt
     1441 ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa
     1501 atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt gttggcataa
     1561 aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg tacctaaatg
     1621 tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag cgttattacg
     1681 taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt gcctatctaa
     1741 catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc aatacaatgt
     1801 aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt cgattccgac
     1861 ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc tagacatcat
     1921 taattcctaa tttttgttga cactctatca ttgatagagt tattttacca ctccctatca
     1981 gtgatagaga aaagtgaaat gaatagttcg acaaagatcg cattggtaat tacgttactc
     2041 gatgccatgg ggattggcct tatcatgcca gtcttgccaa cgttattacg tgaatttatt
     2101 gcttcggaag atatcgctaa ccactttggc gtattgcttg cactttatgc gttaatgcag
     2161 gttatctttg ctccttggct tggaaaaatg tctgaccgat ttggtcggcg cccagtgctg
     2221 ttgttgtcat taataggcgc atcgctggat tacttattgc tggctttttc aagtgcgctt
     2281 tggatgctgt atttaggccg tttgctttca gggatcacag gagctactgg ggctgtcgcg
     2341 gcatcggtca ttgccgatac cacctcagct tctcaacgcg tgaagtggtt cggttggtta
     2401 ggggcaagtt ttgggcttgg tttaatagcg gggcctatta ttggtggttt tgcaggagag
     2461 atttcaccgc atagtccctt ttttatcgct gcgttgctaa atattgtcgc tttccttgtg
     2521 gttatgtttt ggttccgtga aaccaaaaat acacgtgata atacagatac cgaagtaggg
     2581 gttgagacgc aatcgaattc ggtatacatc actttattta aaacgatgcc cattttgttg
     2641 attatttatt tttcagcgca attgataggc caaattcccg caacggtgtg ggtgctattt
     2701 accgaaaatc gttttggatg gaatagcatg atggttggct tttcattagc gggtcttggt
     2761 cttttacact cagtattcca agcctttgtg gcaggaagaa tagccactaa atggggcgaa
     2821 aaaacggcag tactgctcgg atttattgca gatagtagtg catttgcctt tttagcgttt
     2881 atatctgaag gttggttagt tttccctgtt ttaattttat tggctggtgg tgggatcgct
     2941 ttacctgcat tacagggagt gatgtctatc caaacaaaga gtcatcagca aggtgcttta
     3001 cagggattat tggtgagcct taccaatgca accggtgtta ttggcccatt actgtttgct
     3061 gttatttata atcattcact accaatttgg gatggctgga tttggattat tggtttagcg
     3121 ttttactgta ttattatcct gctatcgatg accttcatgt taacccctca agctcagggg
     3181 agtaaacagg agacaagtgc ttagttattt cgtcaccaaa tgatgttatt ccgcgaaata
     3241 taatgaccct cttgataacc caagagggca ttttttacga taaagaagat ttagcttcaa
     3301 ataaaaccta tctattttat ttatctttca agctcaataa aaagccgcgg taaatagcaa
     3361 taaattggcc ttttttatcg gcaagctctt ttaggttttt cgcatgtatt gcgatatgca
     3421 taaaccagcc attgagtaag tttttaagca catcatcatc ataagcttta agttggttct
     3481 cttggatcaa tttgctgaca atggcgttta ccttaccagt aatgtattca aggctaattt
     3541 tttcaagttc attccaacca atgataggca tcacttcttg gatagggata aggtttttat
     3601 tattatcaat aatataatca agataatgtt caaatatact ttctaaggca gaccaaccat
     3661 ttgttaaatc agtttttgtt gtgatgtagg catcaatcat aattaattgc tgcttataac
     3721 aggcactgag taattgtttt ttatttttaa agtgatgata aaaggcacct ttggtcacca
     3781 acgcttttcc cgagatctca tctattgaaa cagcttgata gcctttttca acaaacaata
     3841 ttcgtgctga gttaaccagt gattgatagg tactcttaaa attttcttgt tgatgatttt
     3901 tattttccat gatagattta aaataacata ccgtcagtat gtttatggta tcatgatgat
     3961 gtggtcgtga caatcttaag aacatttagg ttattttatg tatattgaac agcattctcg
     4021 ctatcaaaat aaagctaata acatccaatt aagatatgat gataagcagt ttcatacaac
     4081 ggttatcaaa gatgttctat tatggattga acataattta gatcagtctt tactgcttga
     4141 tgatgtggcg aataaagcgg gttataccaa gtggtatttt cagcggctgt tcaaaaaagt
     4201 aacaggggtc acactggcta gctatattcg tgctcgtcgt ttgacgaaag cggctgttga
     4261 gttgaggttg acgaaaaaaa ctatccttga gatcgcatta aaatatcaat ttgattccca
     4321 acaatctttt acacgtcgat ttaagtacat ttttaaggtt acaccaagtt attatcggcg
     4381 taataaatta tgggaattgg aggcaatgca ctgagagatc ccctcataat ttccccaaag
     4441 cgtaaccatg tgtgaataaa ttttgagcta gtagggttgc agccacgagt aagtcttccc
     4501 ttgttattgt gtagccagaa tgccgcaaaa cttccatgcc taagcgaact gttgagagta
     4561 cgtttcgatt tctgactgtg ttagcctgga agtgcttgtc ccaaccttgt ttctgagcat
     4621 gaacgcccgc aagccaacat gttagttgaa gcatcagggc gattagcagc atgatatcaa
     4681 aacgctctga gctgctcgtt cggctatggc gtaggcctag tccgtaggca ggacttttca
     4741 agtctcggaa ggtttcttca atctgcattc gcttcgaata gatattaaca agttgtttgg
     4801 gtgttcgaat ttcaacaggt aagttagttg ctagaaccca tggctccttt gccgacgctg
     4861 agtagatttt aggtgacggg tggtgacaat gagtccgtgt cgagcgctga ttttttcggc
     4921 ctttagagcg agatttatac aatagaattt ggcatgagat tggattgctt ttagtcagcc
     4981 tcttatagcc taaagtcttt gagtgactag atgacatatc atgtaagttg ctgataggtt
     5041 tccagttttc cgctcctagg tctgcatatt gtacttttcc tcttactcga cttaaccagt
     5101 accaacccag cttctcaacg gatttatacc atggcacttt aaagccagca tcactgacaa
     5161 tgagcggtgt ggtgttactc ggtagaatgc tcgcaaggtc ggctagaaat tggtcatgag
     5221 ctttctttga acattgctct gaaagcggga acgctttctc ataaagagta acagaacgac
     5281 cgtgtagtgc gactgaagct cgcaatacca taagtcgttt ttgctcacga atatcagacc
     5341 agtcaacaag tacaatgggc atcgtattgc ccgaacagat aaagctagca tgccaacggt
     5401 atacagcgag tcgctctttg tggaggtgac gattacctaa caatcggtcg attcgtttga
     5461 tgttatgttt tgttctcgct ttggttggca ggttacggcc aagttcggta agagtgagag
     5521 ttttacagtc aagtaatgcg tggcaagcca acgttaagct gttgagtcgt tttaagtgta
     5581 attcggggta atgactccaa cttactgata gtgttttatg ttcagataat gcccgatgac
     5641 cttgtcatgc agctccaccg attttgagaa cgacagtgac ttccgtccca gccttgccag
     5701 atgttgtctc agattcaggt tatgccgctc aattcgctga gtgtaacgct tgctgattac
     5761 gtgcagcttt cccttcaggc gggattcata cagcggccag ccatccgtca tccataccac
     5821 gacctcaaag gccgacagca ggcttagaag acgctccagt gtggcca
//
LOCUS       DAEEGA010000167         5805 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEEGA010000167 DAEEGA010000000
VERSION     DAEEGA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.168"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="KLH46_003907"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="KLH46_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBE0380402.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="KLH46_003908"
     CDS             578..1027
                     /locus_tag="KLH46_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBE0380403.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /locus_tag="KLH46_003909"
     CDS             complement(1028..1261)
                     /locus_tag="KLH46_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBE0380404.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="KLH46_003910"
     CDS             complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="KLH46_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBE0380405.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1715..1810
                     /gene="yncL"
                     /locus_tag="KLH46_003911"
     CDS             1715..1810
                     /gene="yncL"
                     /locus_tag="KLH46_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBE0380406.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2212..3021)
                     /locus_tag="KLH46_003912"
     CDS             complement(2212..3021)
                     /locus_tag="KLH46_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBE0380407.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3231..4655)
                     /gene="patD"
                     /locus_tag="KLH46_003913"
     CDS             complement(3231..4655)
                     /gene="patD"
                     /locus_tag="KLH46_003913"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBE0380408.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4677..5471)
                     /locus_tag="KLH46_003914"
     CDS             complement(4677..5471)
                     /locus_tag="KLH46_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0380409.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..>5805)
                     /locus_tag="KLH46_003915"
     CDS             complement(5461..>5805)
                     /locus_tag="KLH46_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBE0380410.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1523 a         1473 c         1286 g         1523 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt atatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcaac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagagacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tgcgccattt tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca
     1621 gcaacttgcc cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact
     1681 atacacatag gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt
     1741 actgataaat gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg
     1801 gtttatttga tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga
     1861 cagtacgcaa gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc
     1921 tttttgctgg catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa
     1981 acagcttact gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata
     2041 cgcgcgggag tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg
     2101 cgtaaagtat atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa
     2161 ttgcaaaccc cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc
     2221 tctgaatacc tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatttga
     2281 tatcaatcga ttcatctctt caacaaaata atttatatca tcacacttat tattatccgg
     2341 aagatctgag tagttataat tccaccattt tattctcaac agttcatcaa taacatttga
     2401 atcaaaccta aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac
     2461 atctttggta acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc
     2521 tttcaataca acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt
     2581 ggtaatgaat ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa
     2641 ttcaccgtta taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat
     2701 tatttttaca tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg
     2761 gcaggtcgga aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata
     2821 ttcttcagca ataacattat cttcaattgt tatcttgtca ccttttgaga aaagaaataa
     2881 attcctttct ttatacgact tcttcccctt atgatttaaa aagattttat tatccataca
     2941 aaactcaaag tgttttttcg tccatctgaa ggtaagggtt ttatttttat cttccgtgaa
     3001 tttgtacaat tgaactttca ttgttaatac ttctacttat aaagtattag tttaataacg
     3061 atactctaaa gccttatgct aatcaactgt gacaggatga aaaatcgcca tcaaacattt
     3121 agcctaattt ttacgccagt tattaatgta atcattctaa tacttaaccg ccattaaact
     3181 cagaacctaa aacgatagtg gccgtcctat aacagccact aacatctaac ttaatgttta
     3241 accatgacgt ggcggacgac ggtgtaatcc tccagcccat aaagtgacat atccttgccg
     3301 taaccagaaa gtttctgccc accgtgcggc atttcactta ccagcatgaa atgggtattg
     3361 acccaggtac aaccatattg cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc
     3421 gtccataccg aagatgcaag tccgtactgg ctgtcattcg cccagttcac cacctgttct
     3481 tcgttgtcga agggcgtaac actcactact ggaccaaata cctctttttg cacgatggca
     3541 tcgtcctgta atgcgccagc cagcagcgtc ggcgcatagt aatagccatt acccttgcgc
     3601 ttttcaccgc cagtgatcac tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg
     3661 ctgacgcgtt cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc
     3721 ggcgcaccag attttaacgt tgccaccgca gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaagata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatac ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 acccgttctc cgttaatcag taacttatgt tgcatagctt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgct aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 cagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggat
//
LOCUS       DAEEGA010000168         5780 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEEGA010000168 DAEEGA010000000
VERSION     DAEEGA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.169"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            1..682
                     /locus_tag="KLH46_003916"
                     /pseudo
     CDS             1..682
                     /locus_tag="KLH46_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            941..1125
                     /locus_tag="KLH46_003917"
                     /pseudo
     CDS             941..1125
                     /locus_tag="KLH46_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1268..1489
                     /locus_tag="KLH46_003918"
                     /pseudo
     CDS             1268..1489
                     /locus_tag="KLH46_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1510..1572)
                     /locus_tag="KLH46_003919"
                     /pseudo
     CDS             complement(1510..1572)
                     /locus_tag="KLH46_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            2022..3434
                     /gene="ospC1"
                     /locus_tag="KLH46_003920"
     CDS             2022..3434
                     /gene="ospC1"
                     /locus_tag="KLH46_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HBE0380411.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNKKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(3538..3687)
                     /locus_tag="KLH46_003921"
                     /pseudo
     CDS             complement(3538..3687)
                     /locus_tag="KLH46_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3763..5460
                     /gene="ospD3"
                     /locus_tag="KLH46_003922"
     CDS             3763..5460
                     /gene="ospD3"
                     /locus_tag="KLH46_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HBE0380412.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            5577..5696
                     /locus_tag="KLH46_003923"
                     /pseudo
     CDS             5577..5696
                     /locus_tag="KLH46_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
BASE COUNT         1913 a         1111 c         1162 g         1594 t
ORIGIN      
        1 ccgaacgata tggggcatgg aaaactgttt ataaccgctt taaccgatgg tcaaagtcag
       61 gcgtgattaa tattattttc aacaggttgc tttcgctact tgatgcaaac ggctttattg
      121 actggtctgc caccgcgctg gatggcagta atatccgggc gctgaaatgt gccgccggtg
      181 ctcaaaaaaa catcccgata tcgacggaga taatgggccg ggtcgctctc gcggcggttt
      241 tggcaccaaa atccatctgg caacagacgg aagtggcctc ccgttaaata tcgtgctaag
      301 ccccggacaa gctcatgaaa gccagttcgc gcaacgactt ctggacggga ttggcgttca
      361 gcgtcagaac ggcagcatga aacgccgtgg acatgcggta ctggctgata aagcgtattc
      421 agggcgcgca ttgcgcaacg agctgaaaaa taacggtata aaggcagtaa tcccccgaaa
      481 gtcaaatgag aaaatggcat cggatggacg tgcacagctt gatcgtgatg cgtactgcaa
      541 ccgtaatgtc gttgaacggt gctttgggcg gctgaaagaa tatcgccgca tcgccacgcg
      601 ttacgacaaa acggcgagaa attacctggc gatggtgaaa ctgggctgca tccgactctt
      661 ttatcaacgc ttacgtaatt aagggacaca gcctagtctc caggattccc ggggcggttc
      721 aatcaaagcg cagaacttct gcgtcttctt cgtcaaaaaa atcatcttcg tgaagcttca
      781 cgacatagcg gggaagtttg cttctttgag aggcgggttt acgtttacgg gatttagctg
      841 aacgggccat ataaccacct gaaagacaat gacattgcct gtttttataa cggtaattgc
      901 agaccatgac aaaccgcagc cgtcaggctg cctactcgag tcacaaggct ctttgtgtcc
      961 gatatgccgg cagcgcatta cccatgaaac tggctggaac gtacaccata aggtgaagaa
     1021 agttatgggg gcggtgaaga attatctaac cttgtcttgt tacatccaaa ttgccatcga
     1081 cagttacaca gttgtgaagc cggctctctc aaaaagggct tataaaggct tgagcggtgt
     1141 accgggaaac cggtatgcac cgttcttagg ggagggcagt ccagcaatga actgtcctta
     1201 cccgacaaac atccaaaacg aacggaacgt gctggaatcc gcatataacc cgttgtaaag
     1261 tgaggaaatg gacacctcac ttgctcatga gaacgcccgc ctgcgggcac tgttgcagac
     1321 gcaacaggac actatccacc agatggctga atacaaccgc ctgctctcac agcggatggc
     1381 ggcttatgct tccgaaatca accgactgaa ggcgctggtt gcgaaactgc agcgtatgca
     1441 gttcggtaaa agctcagaaa aacttcgcgc aaaaaccgaa cggcagattc cattttcacg
     1501 ggcgatctac gccacacagg ctctgggttg cagtgactgc tccattaagg cgattttgaa
     1561 ctcataaggg aagtaacaaa aaaactgcaa aagtacaata attatatatg gccaaactaa
     1621 cctctattaa aaaacatcag tttttcacac ttagttgagc ttcatgaaaa aatcatcgag
     1681 gatttcattg cagaatacac attccactaa ctggaagcac tccataagag gaatcagaga
     1741 aagtatcgtt tttttatagt aaaattcatt gctgttcaat aaaattgata tagttattaa
     1801 attatgaaaa taatttcaca aaaaagatta acttgagttc ttaagcatcg gattttagat
     1861 attttgaaat atctggcatc acataacatc aaaagtatat aataccctta tctaattaca
     1921 tgcagtatat tgtatatata ctcaaaaatt gtaattcata ccctgctata tcttgtaggg
     1981 aaagatatta attaaaactg ttttcatata aggttcattt tatgaatata tcagaaacac
     2041 tgaactcagc aaatacccaa tgcaatatag attctatgga taacagatta catacattgt
     2101 ttccaaaagt gacatcagtg cgaaacgctg cacaacaaac tatgccagat gaaaaaaatt
     2161 taaaagatag tgcaaatatt attaaagatt tctttaggaa aactatagca gcacagagtt
     2221 atagtagaat gttctctcaa ggctctaact ttaaatcttt aaatatagca attgatgcac
     2281 catcagacgc taaagcctca tttaaggcta ttgagcacct tgacagatta tcgaagcatt
     2341 atatatctga aataagggaa aaacttcatc ctctttctgc agaggaactc aatttgcttt
     2401 cgctaattat taattctgat ttaatcttca gacatcaaag taattctgat ttgtctgata
     2461 aaattttaaa cattaagtca ttcaataaaa ttcagtctga aggaatatgc acaaaacgaa
     2521 acacatacgc tgatgatata aaaaaaatag ctaatcatga ctttgtgttt tttggcgttg
     2581 aaatctctaa ccatcagaaa aaacaccccc tgaatacaaa acatcacact gttgattttg
     2641 gtgcaaatgc gtatatcatt gatcatgact ctccatatgg atatatgaca ttaaccgatc
     2701 actttgataa tgctattcca cctgtttttt accatgagca ccaatcattt ttagataaat
     2761 tttcagaggt taataaagaa gttagtcgat acgtacatgg aagtaaagga attatagatg
     2821 taccaatatt caatactaaa gatatgaagt tagggctcgg attatacctg attgacttta
     2881 ttagaaaaag tgaagaccaa agcttcaagg agttttgcta tggaaaaaat cttgcccctg
     2941 tggatctgga tagaatcata aactttgttt ttcagccaga gtaccatata cctaggatgg
     3001 taagtacaga aaacttcaaa aaagttaaga ttagagaaat atccttagag gaggctgtta
     3061 cagcatctaa ttacgaagaa attaacaagc aggtcactaa caaaaaaatt gctctccagg
     3121 ctctttttct ttcgattact aataaaaaag aggatgtcgc cttatatata ttatctaatt
     3181 ttgagataac tagacaagat gttatttcca taaagcatga gttgtatgat attgagtatc
     3241 tacttagcgc tcataattca agctgtaaag tacttgagta ttttatcaat aagggattgg
     3301 ttgatgtaaa cacaaagttc aaaaaaacta atagtgggga ttgtatgttg gataacgcaa
     3361 taaaatatga gaatgcagaa atgataaaac tattattgaa atatggtgca acatctgaca
     3421 ataaatatat ttaatcaaaa ttgaatatcg tttagcaaaa ggcagtattt gcacaaaata
     3481 tcgatatatt gtttattgtc agtatggctc aatgtgataa tagctggaaa gtttgatggg
     3541 tttcgccccg ttgtagcggt agtcgaactg gaaggtcttc aggcactgct tacagcgata
     3601 gagcagcccc cctgaactgg aatggccgtt ccggataccc ccctgagttt cagagtaacg
     3661 gtgacaaacc acatcaatct ttgccatcaa tcatccaaag ggcaaagagt acaacaacac
     3721 taagtctgcg tcacaaccca tcaatgaaag gaatatatac atatgccatc agtaaattta
     3781 atcccatcaa ggaaaatatg tttgcaaaat atgataaata aagacaacgt ctctgttgag
     3841 acaatccagt ctctattgca ctcaaaacaa ttgccatatt tttctgacaa gaggagtttt
     3901 ttattaaatc taaattgcca agttaccgat cactctggaa gacttattgt ctgtcgacat
     3961 ttagcttcct actggatagc acagtttaac aaaagtagtg gtcacgtgga ttatcatcac
     4021 tttgcttttc cggatgaaat taaaaattat gtttcagtga gtgaagaaga aaaggctatt
     4081 aatgtgcctg ctattattta ttttgttgaa aacggttcat ggggagatat tattttttat
     4141 attttcaatg aaatgatttt tcattccgaa aaaagcagag cactagaaat aagtacatca
     4201 aatcacaata tggcattagg cttgaagatt aaagaaacta aaaatggggg ggattttgtc
     4261 attcagcttt atgatcccaa ccatacagca actcatttac gagcagagtt taacaaattt
     4321 aacttagcta aaataaaaaa actgactgta gataattttc ttgatgaaaa acatcagaaa
     4381 tgttatggtc ttatatccga cggtatgtct atatttgtgg acagacatac tccaacaagc
     4441 atgtcctcca taatcagatg gcctgataat ttacttcacc ccaaagttat ttatcacgcg
     4501 atgcgtatgg gattgactga gctaatccaa aaagtaacaa gagtcgtaca actatctgac
     4561 ctttcagaca atacgttaga attacttttg gcagccaaaa atgacgatgg tttgtcagga
     4621 ttgcttttag ctttacaaaa tgggcattca gatacaatct tagcatacgg agaactcttg
     4681 gaaacttctg gacttaacct tgataaaacg gtagaactac taactgcgga aggaatggga
     4741 ggacgaatat cgggtttatc ccaagcactt caaaatgggc atgcagaaac tatcaaaaca
     4801 tacggaaggc ttctcaagaa gagagcaata aatatcgaat acaataagct gaaaaatttg
     4861 ctgaccgctt attattatga tgaagtacac agacagatac ccggactaat gtttgctctt
     4921 caaaatggac atgcagatgc tatacgcgca tacggtgagc tcattcttag cccccctctc
     4981 ctcaactcag aggatattgt aaatttgctg gcctcaagga gatatgacaa tgttcccgga
     5041 cttctgttag cattgaataa tggacaggct gatgcaatct tagcttatgg tgatatcttg
     5101 aatgaggcaa aacttaactt ggataaaaaa gcagagctgt tagaagcgaa agattctaat
     5161 ggtttatctg gattgtttgt agccttgcat aatggatgtg tagaaacaat tattgcttat
     5221 gggaaaatac ttcacactgc agaccttact ccacatcagg catcaaaatt actggcagca
     5281 gaaggcccaa atggggtatc tggattaatt atagcttttc aaaataggaa ttttgaggca
     5341 ataaaaactt atatgggaat aataaaaaat gaaaatatta cacctgaaga aatagcagaa
     5401 cacttggaca aaaaaaatgg aagtgatttt ctagaaatta tgaagaatat aaaaagctga
     5461 atattatctc gtggtgatac actgaatttg aggtaaccgg ctcatttaaa ccgtctggtc
     5521 tgttacctcc ggttccacaa aaataatgtc catcattttg aagtggacac tatcgtatgg
     5581 aataccggac ctggatcact gaagctttac gccttcattt cgaagaacat ttaccacggg
     5641 ttgtggccgg acgtcgcctg ggcgtaccaa aatcaactgt ctgtggtatg ttcgtgtgaa
     5701 ccgcccccgg tatcccgctc atgctggcga acccagacac gcagtgaacc gccccgggtt
     5761 tcctggagag tgttttatct
//
LOCUS       DAEEGA010000169         5760 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEEGA010000169 DAEEGA010000000
VERSION     DAEEGA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.170"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..403)
                     /locus_tag="KLH46_003924"
     CDS             complement(<1..403)
                     /locus_tag="KLH46_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HBE0380413.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTAL"
     gene            complement(548..1051)
                     /gene="cheW"
                     /locus_tag="KLH46_003925"
     CDS             complement(548..1051)
                     /gene="cheW"
                     /locus_tag="KLH46_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBE0380414.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(1072..3036)
                     /gene="cheA"
                     /locus_tag="KLH46_003926"
     CDS             complement(1072..3036)
                     /gene="cheA"
                     /locus_tag="KLH46_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBE0380415.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(3041..3967)
                     /gene="motB"
                     /locus_tag="KLH46_003927"
     CDS             complement(3041..3967)
                     /gene="motB"
                     /locus_tag="KLH46_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBE0380416.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3964..4851)
                     /gene="motA"
                     /locus_tag="KLH46_003928"
     CDS             complement(3964..4851)
                     /gene="motA"
                     /locus_tag="KLH46_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBE0380417.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGVLIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(4978..5556)
                     /gene="flhC"
                     /locus_tag="KLH46_003929"
     CDS             complement(4978..5556)
                     /gene="flhC"
                     /locus_tag="KLH46_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBE0380418.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRARTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(5559..>5760)
                     /locus_tag="KLH46_003930"
     CDS             complement(5559..>5760)
                     /locus_tag="KLH46_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBE0380419.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         1342 a         1552 c         1407 g         1459 t
ORIGIN      
        1 ttaacgctgt gtaatagttt ttatattttt catcaaaatt acggctggta gcgaccattt
       61 caggtaacgg tgccatgctt ttgaattttt tataatgcgt cgcggcctgg gccaatgttt
      121 tcctagcgct atcgagcaat tcaactttgg cgttactttg ttgattggag gaatccatca
      181 tcatccgcac cgctgaacga ctcaggttaa tgcgcgtttg cagcattaaa tcccaggttg
      241 acgtcagctc gccctgctgt tcccgtaatt gattggaaac cacaaagctc ttctggctat
      301 ggtgaaggga agaaaaaaac aaactgccgg aaataagctg taacagtgcg aataccccca
      361 gcaccattac caacagcgtg actacgcgga tacggttaat cataaggcac cttcctgaaa
      421 acaagttgat ctcgttatcg gcaagaaggg gggaaacttt attgctgatg ccacccgccg
      481 cgaaattgaa ataaaaaacc cgatgcgcag atcatcgggt tcatttcaat tgaggaaatc
      541 gggagaatta cgccacttct gacgccgcgc tatctaacag cgccatctct tcgctgttca
      601 gcagtttttc gatgttcacc agaatcaaca tacggtcgcc cagtgcgccc agtccagtga
      661 gatattctgt tgaaagcgtc acggcaaact ctggtgccgg acgaatttgc tccgccgtca
      721 atgaaagcac gtctgaaacg ccgtcaacca cgatgccgac cacccgctgt ccgagattca
      781 ggacgataac taccgtgttg tcgttatagt ccacatccac ctggctgaac ttaattcgta
      841 agtcaacaat cggcacaata acgccgcgca gattcgtgac gcctttgata aacgctggcg
      901 tgttcgcaat ccgtgttacc tgatcgtagc cacggatctc ctgcactttc aggatatcaa
      961 taccgtactc ttcatcacca agggtaaata ccagaaattc ctggcctgac ggctcgctgg
     1021 ccagctttgt tacattcgtc ataccggtca tattgttacc tttttactca ttcaggcggc
     1081 ggtgttcgcc atacgttgtt cgcggtttat cgcctgcaag gcggagacat caacaatcag
     1141 tgccacgctg ccgtcgccaa gaatggtcgc agcagaaatg ccggggactt tgcgatagtt
     1201 actttcaagg tttttaacca caacctggtg ttgaccaatt aattgatcca ccagcaaggc
     1261 gtagcggcga ccgccacttt gtaagatcac cacaattccc tgggtggctt cggttttcgc
     1321 gcccgcgacg ttgaacactt tccacagttc gacgatgggc agatattcac cccgcacttc
     1381 cagcacccgc tcgccgccgg ccagtggatg gagatcggct tcacggggtt gcagtgattc
     1441 cataacagca ttcagcggca gaatgaaaac ttcatccgca acgcgtacgg acatgccgtc
     1501 gaggatggcc agcgtcagcg gcagtaaaat gcggatcgta gtgccagtac cctgcttcga
     1561 ctggatttcg acatgaccgc ccatctcctg gatattacgt ttaacgacgt ccatgccgac
     1621 gccgcgcccg gagacgtcgg tgacctgctc tgccgtggag aagcctggtg caaatatcag
     1681 cattgcaact tcgtcgtcgc tcatgttttc gctgacagtc aaaccttgcg aggccgcttt
     1741 tgccagaatt cgctcacggt ttagccccgc cccatcgtcg gtcacttcaa tgcaaatgtt
     1801 gccgccctga tgttcggcag acagaattaa atttccgacg ctgtttttac ctgcggcgag
     1861 ccgtttttct ggcagttcaa taccgtgatc gaggctattg cgtaccaggt gggtcagcgg
     1921 gtcgataatg cgttctatca ggctcttgtc gagctcggtg gaactgccca ccagcgtcag
     1981 ttctacctgc ttgccgagtt ttcccgccag atcacgcacc agccggggat agcgactaaa
     2041 gacatattcc atcggcatca tgcgaatcga catcactgat tcctgcaaat cacgggcgtt
     2101 acgttgtaac tgccccatgc tggtaatcaa atcaccatga ttaaccgggt ccagttcgct
     2161 ggaacgctgg gcaagcatgg actgggtgat aaccagctcg ccgacgaggt taattaattg
     2221 atcaaccttt tctaccgcta cacggatgct ggtggattca ctgctgcgcg tcgttttttc
     2281 ccgctccacg cggccggttg gcgcttgttc ggctgccagt ttaagcacag gtggggtagc
     2341 tatttttggc gaaacttcta ctgtttcaaa ggtaatctga tcggcttcaa tcacaaaaca
     2401 gagtaccgct gtgatgtcat cttcggcgat atcgcccggt aatattgccg agagagaatc
     2461 cgcccctttc accacgtcat ttaacgttgt cagatgcccc agctcttctt ccagcaggtc
     2521 gacttcgccg gccttcaggc gcgaaaggat aattcgtcgc ggcaactgac tgcgactttg
     2581 ctcatcttgc ggttcacttt tggcaaccac acttaatcgg gtcactgcgg atggcgtttc
     2641 gcctttcgct tctaatgcca gttgacgcaa ggcctggcag atataatcgt agctggcggc
     2701 atccggctct tgcgactgtt tataagcgtc gagctgttct tgcatgatgt ccttcgtttc
     2761 caaaaacaga ttgataatgt cggtgttgag ttgcatctca cctcgtctgg cttcatcgag
     2821 caggttttcc atcagatgcg tggtttcctg caaaacgctg aagccaaaag ttcctgcccc
     2881 tcctttgatc gagtgggcag cccgaaagat ggcattcaat tgttcggcat ctggcgcttc
     2941 cggctgcaaa accagcagat gctgctccat atcagccaac agttcgtccg cttcatcaaa
     3001 aaatgtctga taaaaatcgc ttatatcgat gctcacgctg tcacctcggt tcggctgatg
     3061 gcattgtggg aacactgacc tgtggtgcaa cctcaggttt ttctagggcg cttactggct
     3121 cattctggct ttcggcgttt tcatgcaaaa tggcctgttc ggcttgtttg ttcagtacca
     3181 acaggctgat gcgacggttg acggcatcat caggtccgcg atcgcttaag cgcatcgttg
     3241 ccgccatgcc gacgacacgt aacactttgc cgctatccaa ccctccgacc atcagttcgc
     3301 ggcgggatgc attggcccga tcggcagaaa gctcccagtt gctgtaacct ttctcaccgc
     3361 tggcgtaggg aaaatcatcg gtatgacctg aaaggctaat acggttggga ataccgttca
     3421 gcacaggcgc gatggcgcgc agaatgtcgc gcatataggg ttcgacatcg gcactgccgg
     3481 ttctaaacat cgggcgattc tggctatcga tgatctgaat acgtagacct tcctggacca
     3541 gatcgatttt gagatggaga cgtaacgccc gcagtttcgg atcggactct atcaactggt
     3601 cgagatcacc ccgcaatttc cgcaatcgac tttgctccat gcgttttttc agctcttcga
     3661 tgttcggctg cttattcact tccccctggc tctgggtgta atcatcacca ccgccgggaa
     3721 ttgggctttc actattggaa atgcgatcgc cgcccgtaac cgctgtcgcc agtggagtcc
     3781 ggaagtactc cgcaatctga atcaactctt ttgggctgga gatggagatc agccacatca
     3841 ccaaaaaaaa ggccatcatc gcagtcataa agtcggcata agcaatcttc cacgatccat
     3901 gtgctgcccc gtggcttttg gctttgcgtc gtttgacgac aataatcgga tgcgcttgat
     3961 tcttcatgct tcctcggttg tcgtctgttg ttgcggattt ttcaccgcac gcacatgctc
     4021 ttccagttca ataaacgacg gacgttcgct ggaatagagc gttttgcgac caaactcaac
     4081 ggcgataggc ggtgcgtaac cgttcagatt agaaagcaga gtgactttga cgcactgcat
     4141 cattttgctg gtttcggcgc ttttctgacg taaaacactc gctagtgggg aaataaatcc
     4201 gtaagccaat aaaataccga ggaaagtccc caccatcgca tgtgcgataa gcacccccag
     4261 ctcggcggca ggacgatcgg ctgaacctaa cgcgtgaacg ccccccatta cagccgcgac
     4321 aataccaaac gccggaagtg agtccccgac cagcgccaga ctgttcgccg ggacttctgc
     4381 ctcgctttcg tgcgtctcaa tctcttcatc catcagggct tcgatttcga aggtgttcat
     4441 gtgaccgctg ataatcaggc gcagataatc gacgataaaa tcaagcatga cgctatccgc
     4501 gaggatgcgt gggtagctgg cgaagatctc gctctcacgg ggattttcaa tatcacgttc
     4561 cagcgaaaac atccccatct gccgcgattt cgccatcaac cgataaagca gagccagcag
     4621 atccatatac attgctttgg tgtatttgga gcgacgaaac agcaacggca gcgcttttag
     4681 cgtgccttta atcgctttgc cattattgcc gacgataaac gacccaatcc ctgcaccggc
     4741 aataatcacc agttcagcgg gttgatagag tgctccaagg cttccaccgg tcatcaaata
     4801 accgccgaaa actgtaccga gaacaaccag gtaacctaat aagataagca cgacatcatc
     4861 cttccactgt tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc gattggggaa
     4921 aaaaaaagca gcggtacgtc gttaccgctg ctggaatgtt gcgccacacc gtatcagtta
     4981 aacagcctgt actctctgtt catccagcag ttgtgggata atatcggcag gattctggga
     5041 aagtttacgt ctttttactg cccgggatgg cggttgacat aagctgcagg caaagctgcc
     5101 aacaggctgg tgagcgtggg taataaaatt gccgccgcag cagttgcagc tggaaagttg
     5161 cagtaatcca ctttcaacaa accgcaccaa tgtcctggca cgggttaatg ccagcagtgg
     5221 tccttcttct gcttgtgggc actgttcaag gtataaacgg taggctttga tcaccgcatc
     5281 gacgccatta cacaaaccgg ttttcagtaa aaactgccat gcattacaga acatcgaagc
     5341 atgaacgttt tgttcccagg tcataaacca gtcggttgag aatggcagca tgcctttcgg
     5401 cggtgggctt ccgcgcagtt ctttataaag ttttatcagg cgtccgcgac ttaactgtgt
     5461 ttcgctttcc agcatctgca aacgagcgcc cagggtgatc aattccattg ccagctgaat
     5521 atcccgcgct tcctgaacaa tgcttttttc actcatgatc aggccctttt cttgcgcagc
     5581 gcttcttcag gctgattaac atcattcagc aagcgtgttg agagcatgat gccggtatga
     5641 atttgctgga gatcgtcaac gcgggaatct tgcgtcaact gagtaatcgt ctggtggctg
     5701 tcaaaacgga agtgacaaac cagctgattg gtttctgcca gcttaaccat ttgcggaaga
//
LOCUS       DAEEGA010000170         5745 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEEGA010000170 DAEEGA010000000
VERSION     DAEEGA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.171"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            1..173
                     /locus_tag="KLH46_003931"
                     /pseudo
     CDS             1..173
                     /locus_tag="KLH46_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            699..1877
                     /gene="ltrA"
                     /locus_tag="KLH46_003932"
     CDS             699..1877
                     /gene="ltrA"
                     /locus_tag="KLH46_003932"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBE0380420.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFSFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1960..2139
                     /locus_tag="KLH46_003933"
                     /pseudo
     CDS             1960..2139
                     /locus_tag="KLH46_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2169..2651)
                     /locus_tag="KLH46_003934"
                     /pseudo
     CDS             complement(2169..2651)
                     /locus_tag="KLH46_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2907..3887)
                     /locus_tag="KLH46_003935"
     CDS             complement(2907..3887)
                     /locus_tag="KLH46_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBE0380421.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3887..5086)
                     /locus_tag="KLH46_003936"
     CDS             complement(3887..5086)
                     /locus_tag="KLH46_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBE0380422.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5408..>5745)
                     /locus_tag="KLH46_003937"
     CDS             complement(5408..>5745)
                     /locus_tag="KLH46_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0380423.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1573 a         1315 c         1370 g         1487 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtggagg tgggccctcc ggtcagggtt tacaggagca
      241 cagaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtggggttc gggaagctga acgaaagtaa actgtccgat
      361 gaagcatcga aatcttagat gatgccaaaa tggggccgta cagccgtccc ctgataagcg
      421 cagaagacac ctgcttactg actgcgcggc atccggtgtg aaggcagcag gatcccgggc
      481 cagaccatct cagggaacgt gagaacctgt ttccggcacg tatcgggaaa caggggcgga
      541 cccattcgta gtagtgatga aagatctgta atgggtctgg agcgaaggga tggggatcac
      601 attcattgca gaagacacaa cccaaaaggg gatgacgtaa ctgatgcaac gaaaatcatt
      661 tgaaatacca aaagcactgg tctgggcatc atatctggat gtgcgtcgca acaagggagc
      721 cccccggatg cgacgggcag actttgaaaa tgttcgacca gcaacgtgat ggcaatctat
      781 acaagatctg gaaccggcta tgttcgggaa catggtttcc gccaccagtg ctggaaaaac
      841 ggatcccgaa gtcgaatggc aaggaacgca ttctggggat cccaacagta tccgatcgaa
      901 ttgctcaggg agcgataaaa cttttcatgg aagaaaagct tgacccgatt ttccatgctg
      961 attcatatgg ttaccgacca ggcaaatcag ctcatgacgc tctgaaacaa tgtgccatcc
     1021 ggtgctggcg ttatagctgg atactggaag tggacatcag tgcgtttttc gaccatgtga
     1081 gacatgatct ggtgctcaaa gctctggaac accacgggat gcctaaatgg gtcatcctgt
     1141 actgtcgacg ctggatggaa gcgccaatgc agagttgtga aaatggagaa ttaataaccc
     1201 gaacacgagg cactccgcag ggcggggtca ttagtccgct acttgctaac ttgttccttc
     1261 actatgcatt tgatttgtgg atggaaagag aatatcgggg ggtaccgttt gagaggtacg
     1321 ctgacgatat tgtagtacat tgttcacgaa tgagtgatgc aacaaggcta aagaacaggt
     1381 tatcggagcg cttctcggaa gtcgggctgg tcctgaatgc agggaaaacg aatatcgcct
     1441 acattgacac gtttaaaagg agaaacgtcg caacgagttt ctcctttctc ggatatgact
     1501 tcaaagtgcg tacgctgaag aatttcaaag gcgaacggta ccgaaaatgc atgccgggtg
     1561 cgtcaaatgc agcaatgcgc aaaataacag aaacaatcaa gaagtggcgt atacatcgct
     1621 caacagctga gagtttgctg gattttgcga ggcgctacaa tgcgatagtg agaggctgga
     1681 tcgagtacta cggaaagttc tggtccagaa atttcaacta tcgactgtgg agtgcaatgc
     1741 agtcacgtct gctcaagtgg atgcagtcta aatacagact ttcgaaccgg aaggctcagc
     1801 gaaagctgac gctggtaagg aaggagtatc cgaagctatt tgttcactgg tatttactac
     1861 gtgcatcaaa tgagtgatca agagccgtat gacgcgagag tgtcacgtac ggttccgtga
     1921 gcagccgggg agtgaaattc tctccggctg actcgactcg cgggttccga cagcgatgac
     1981 gaacatgcgg cggtgttgta ctcgctgatc ggcacatgcc gtctgaacaa tgtggaacca
     2041 gaaaaatggc tgcgttacgt cattgagcat atccaggact ggccggcaaa ccgggtacgt
     2101 gatctgttgc cctggaaagt tgatctgagc tctcagtaaa tatcaatacg gttctgacga
     2161 gccgcttact tttggcgtaa ttctgctgcg cctttactga acacaatgcc gtattcagca
     2221 agaagtccac gaatgtgatt tatcagttgt gtcctttcca ttgaaaccaa ccgacgcata
     2281 ttcaaaaaag tctggacgtc ctgttgctcc agagttttta ttggtacggg tcgtatagtt
     2341 ccatgttggc aggcctcagc tattgcctgt gcgtcgttat aatcattttt ctggcaccgc
     2401 tgataagctt taacatgttg tgccggaagc aatattacat catggtcgag tttcctgatt
     2461 tcccgggccc agtaatgaga tgccccgcca gcctccatcg caacagtcat tttaggctgt
     2521 tgcaccagcc agttaagcag ttgattgcga gaaagtttgg tctttcctgc gggtttgcca
     2581 tgagcattga tactgaaaag ataaaagttc gttttagcca gatcaatgcc ggtgattttc
     2641 tgttcagaca tggtatcccg cttacttttg ttgttaaagt ttatccatca tggcgcaatt
     2701 gaaggctgcc gtgaaagagg gggaagacta tctcatcggt tactgttaaa cggctaagag
     2761 aaactatctt tttcaagcaa agaagatacg gtgaaacagg cgatttcacc gtgaaatact
     2821 gttcaaaaaa aacgctaata aaagcaaaat ttcttttaaa acagccaatt aaaaactggt
     2881 gagttgctgg cgaaaaactt gaattttcac agcgaattca gattatcttt tagtacaaga
     2941 gcaatcatac gatctaactt atcctgaact tccaacggta atctgccgaa ttcatatgag
     3001 aacgtacgtc cctttactcg ttttcgagca aatttatctt tactatcaaa tttccataac
     3061 aaagttacaa ccgctttatc ctttactcct gaatcaagca atgaagacat ttccttggtt
     3121 ataagccgca taatactgtt tttaacctcg tcttcagaaa gattgtcatc agcagtaata
     3181 agctcaatac tcggcgagat attcttcaca acttcatcaa tagatatatc acccagcctc
     3241 agtcgttcac ttaattcagc cagttgcctg taatcggcgt atgtcagctc agactgaaca
     3301 ggaaaaagag acacaaaatc tttaggtacg gacgctgctt ggagggcacg agtcacctta
     3361 gctgccgaca atccctctct ttccgcaatt tgcttctggt tcataccggc ctctttgaga
     3421 cgtactaaac gcaacccaat ttcacgaata ttatgttcaa gtgacgtctg caaatctttc
     3481 gccagatgct gagcttcaga aacagtaagc tcatcatccg ttacaagtac acgtaatcca
     3541 accttgcata gcaaagctgc tgcccgacga cgggaaccat ccaaaatctc aataagatcg
     3601 ccagtccgaa tcccaataca tggatagaat tgttgtaaac gtatagttct ggtgatgtct
     3661 tttagtgagt ccggcgtcag ggctgtctgt tcgcggccat tcacctcttc aaccacaaaa
     3721 gtttgagtat ccactttgtc atgatcaacc ttgatctcag taaattttgc ctttcggcca
     3781 gtattgaggg taaatacatg aactggagcc gatatttctt cagtattatt caggatattt
     3841 gtattcagcg tacggccaat agtcggtcgg tgctttctgt tttccatcag tagttggccc
     3901 ttataaattc aatacgatca aaaactgctt tagcgaaatc ttctgctgcc atacgtgcat
     3961 ttttcaaagc ttcaccactg ccaacataag taacaggatt cgcagatatc acggtatcaa
     4021 acgactcgcc gcttctctca aaaccatcaa gacgtggcat tgaaacatca agcatgtcac
     4081 caccgaaaat ctcttttgtc aggctatggc agtatttatg atctgactta ttcgccagtt
     4141 tcgacatgaa gccaatattt gcctgcaaac ggcatgaaca tccatcctgt tcaataatct
     4201 gtacaagttc aggtaaacga gccaaatatt taagcgtgga atggaaatca acttgtgctg
     4261 gcggcaccgg tgtaaacatg atatctgcgg cagcaatggc atttttcaaa aatgcgtcca
     4321 gatgcgggcc tgtatcaata aggatgaagt cgaaatcatg cttcagctta tcaataatat
     4381 tctcacgcaa tatcgcgtgt ttgttttgtc ctaatagatg ctcctcgcac agagtatccc
     4441 agtttgatgc aataaaggcg tcgtcaatag aagccggcat aacataaacg ccagggataa
     4501 ctgacggaac gataaaatct tccagtaatt cctcacgact cacgttctgc aacatagcct
     4561 gcggtgcggt tgtatcaacc agccccaccg catgcaagta gttcagaaac atagtggctg
     4621 atgactgggg atctaaatca aggagcagaa tacgtaaatc ttctgacagc aggtgaggat
     4681 gagcacgcag ggcgtgagct acggaaacac tactcacggt tttagacaca ccgcctttta
     4741 gactaccgat gaatatggaa tatgcctcac tataacgatc gcgatattta ggaatgccac
     4801 gatgtgcata gatatcgata atgttctgga tggttaatgc atattttgtc gcggttcctg
     4861 ctgggcgttt ttcaaactga tagccctgag cctccatctc accgactgca ccatccacgc
     4921 ttgcgcgcgt taattttgga agcttagcta cagcagactt tgaatagact tgataaaaaa
     4981 catcagcctg aaactcctgc ttttgctcct gaatctgttt tgttagggcg agcaacattt
     5041 tatctgcacg ctgcgcaacc ttacttaact gctcaaaact agtcattccc cctccgtgct
     5101 atgtaggatt ttaacttatt acaaaaaaaa ctgcattagc agggtaatgc atgcaagtac
     5161 atattgtaca tgcttatatt aaaaatgcgc gcattgctga tttattaatc atcggaatga
     5221 atattactgt aagatagtat gcctttccgg gagaaagacg ctgatttccc ccgaataatc
     5281 cagttcacca ccacactccg gacaacagct ttcttcaggc tccagatggt gtatttcgcg
     5341 ggaaacatgt tccggtaacg gacggcgatg acgtgactgt cgcaacaggc gggggtagtg
     5401 cttccaatca gtagaacatg tgcttttcaa tgaaggagcc gatgactttt tcgtggattt
     5461 ccacggaacg cgagaagcag attgttttac gtgccagccg cttgatgcgg gtgcggaggg
     5521 tccggttgtt acgtgcaata cgttgcgtga agattttgcc ggtcaggtgt ttctccttcg
     5581 gcacttctct ggcgtagctg ccccagtcat cgctggtcac catgccgata cagaacggag
     5641 tcaacagggc taacagttcc cgacaggtct catcattccg tggcccgaaa gtgtaagcca
     5701 acacaccgcc agttttagtg ttgtacgcat accagagcca gtgtt
//
LOCUS       DAEEGA010000171         5737 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEEGA010000171 DAEEGA010000000
VERSION     DAEEGA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.172"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..241
                     /locus_tag="KLH46_003938"
     CDS             <1..241
                     /locus_tag="KLH46_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380424.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="KLH46_003939"
     CDS             complement(238..1179)
                     /locus_tag="KLH46_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HBE0380425.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="KLH46_003940"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="KLH46_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="KLH46_003941"
     CDS             complement(1684..2904)
                     /locus_tag="KLH46_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBE0380426.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPCKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="KLH46_003942"
     CDS             complement(2908..3651)
                     /locus_tag="KLH46_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBE0380427.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="KLH46_003943"
     CDS             complement(3542..5008)
                     /locus_tag="KLH46_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HBE0380428.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5241)
                     /locus_tag="KLH46_003944"
     CDS             complement(5008..5241)
                     /locus_tag="KLH46_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBE0380429.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5229..5708)
                     /locus_tag="KLH46_003945"
     CDS             complement(5229..5708)
                     /locus_tag="KLH46_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380430.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1227 a         1618 c         1435 g         1457 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca accatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacatg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatga
//
LOCUS       DAEEGA010000172         5702 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEEGA010000172 DAEEGA010000000
VERSION     DAEEGA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.173"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..219)
                     /locus_tag="KLH46_003946"
     CDS             complement(<1..219)
                     /locus_tag="KLH46_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBE0380431.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
     gene            complement(216..1124)
                     /locus_tag="KLH46_003947"
     CDS             complement(216..1124)
                     /locus_tag="KLH46_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBE0380432.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAVFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1320..2087
                     /locus_tag="KLH46_003948"
                     /pseudo
     CDS             1320..2087
                     /locus_tag="KLH46_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="KLH46_003949"
                     /pseudo
     CDS             complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="KLH46_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="KLH46_003950"
     CDS             complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="KLH46_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBE0380433.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            5533..>5702
                     /locus_tag="KLH46_003951"
     CDS             5533..>5702
                     /locus_tag="KLH46_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380434.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
BASE COUNT         1339 a         1480 c         1470 g         1413 t
ORIGIN      
        1 ctgttgcaac cagctcagag ccgccagcga ttgctgtgtg gcttcaacca ccggacagga
       61 gcgcgttttc aggctgatca ccagtgcgtc gattgcttcc ggcattttac ctgttggaac
      121 accgttaatt tgtaccgttg gtagaccgtt ttccaccaga aaactggcga tatccgtcgc
      181 gccggtaaaa tcatcggcga taacgccaat cttgatcatg atttcgctcc cggtagagtg
      241 atgccagaga aaatcttgat aactgcgcta tcgtcttctt tcccgtaacc cgcgttactg
      301 gcgctggtga acatattcaa tgctgttgag gccaatggca gcgggaagtg cagggctttg
      361 gctgtatcgg caaccagacc aagatcctta acaaaaatat cgacggctga atgcggggtg
      421 taatcgccat ccaccacatg acgcatccgg ttttcgaaca tccaggaatt tccggcggca
      481 ttggtcacga cgtcatacat cacatccagc gggatccccg cacgggctgc aagtgccatc
      541 gcttcggctc cggcagcaat atgtacgccc gctaacaact ggtgaataat ttttacggtc
      601 gaacctagtc ccggttctgc acctatgcga taaacttttc cggcaacggc ttccagcacg
      661 ggtgccagtc gttcaaaggc aatatcgcta ccggaggcca tgacagtcat ttcaccgtta
      721 gcggctttta ctgcaccacc agaaactggc gcatccagca tttccagatc gaatccagcc
      781 agagcggtag caatttcttg cgcatcagca ctagcgatag tggaagaaac cattactgcc
      841 gtaccgggtt tcagatgttg tgcaacgcct gtttcaccaa acagcacctg tttaacctgg
      901 gccgcattga ccaccagcac cagcagagcg tccagttttt cggcaaacgt cgcggcgtta
      961 tcagaaaccc cgcaagcacc tgcctctttc aacgtagcgc aggcattgct gttcaggtct
     1021 gcgccccagg tagaaaaaac tgcgcggaca catgacagtg ctgctcccat tcccattgac
     1081 cctaagccaa cgataccgac atgaaactca gatcccgttt tcatctgctc tccttgttaa
     1141 tttaagtgat attttgtttg atattgtgaa cataagcgct gggagataac gatatggtga
     1201 gatgattcac ataaattaac attttgtgtt attttatgtg aactaagcgt tagttgcgcc
     1261 gcgcacgttt cgcaggcaaa tagcgtagaa tgtcagcagg acaaaggaag gagcaaaagt
     1321 tgatacccgt agagcgtcgc caaatcatcc ttgagatggt agctgaaaaa ggcattgtca
     1381 gtattgctga actaacggac agaattaatg tgtcacatat gaccattcgt cgggatttac
     1441 aaaaactgga gcagcaggga gccgttgtgc tggtgtccgg aggcgtctag tctccgggac
     1501 gcgtggcgca tgaaccttct catcaggtaa aaactgcgct ggcaatgacg caaaaagcgg
     1561 ctattggcaa gctggcggca agtcttgttc tgccgggaag ttgtatctat ctggatgcgg
     1621 gaacgaccac gttagcgata gcacagcatc tgattcacat ggagtcactg actgtggtca
     1681 caaacgattt cgttattgcg aactacttgc tcgacaacag taattgcaca attattcaca
     1741 ctggcggtgc agtgtgtcgg gaaaaccgtt cctgtgtcgg ggaagccgct gcgaccatgc
     1801 tgtgcagcct gatgattgat caggctttta tttctgcatc gtcatggagt gtgcggggga
     1861 tttctacgcc agcggaagat aaagtcacgg tgaaacgggc gattgccagt gccagccgcc
     1921 agcgagtttt ggtctgtgat gcgacgaaat atggtcaggt ggcgacatgg ctggcgttac
     1981 cattaagcga gtttgatcag attattacag acgacggttt gccggagagt gccagtcgcg
     2041 cgctggcgaa gcaggatctc tctttgctgg tagcgaaaaa tgaataatgg cctgcaataa
     2101 catctggtta ctcatgcttc acagaagaag catgagacta ccttatttta taaaatgaca
     2161 gccgcccact tttcggcgat ccggtatcaa tataaatctg gttagcgaac gtctgaatgt
     2221 tatcaaacat catatgtcca aatataaaat aatcagcgcc gtttatttgt tgtaactcgc
     2281 cattaagcga tttctgcaca cgataaacag gccagagtaa ttcgctctcc gctatttctt
     2341 tgccaaagag atattcatca cccggataat ctgcatgtgc gatgacatat tttattatgt
     2401 cgttagtgat ttcaataata tgtggaaggt gatggaattt cagcaacaga tctattgcct
     2461 cttgttgcaa tcatttaaat cgaaaaacca gtcaccaccg ctggcaagcc acatattgcc
     2521 atcgccggtc gcgaacgcat ccagcgccat tgcttcgtgg ttgcctttaa cggagataaa
     2581 ccagggctgg tttagcaggc gcaggacgtc aagactctcc ggcccacgat caatattatc
     2641 gccgacagaa ataagtaagt cggtttcggg gaaaaaagag agttgatgta agcgggattg
     2701 taataactga tattcaccat gaatatcacc aacgacccat atatggcgat agtgatgggc
     2761 attgattttt tggtagcgtg tagatggcat ggttttaccc tgtaaaataa gctttcctat
     2821 tatacagggt atttttattt gattcgtcag ttgtcgttaa tattcccgat agcaaaagac
     2881 tatcgggaat tgttattaca ccagactctt caagcgataa atccactcca gcgcctgacg
     2941 cggagtcagt gaatccgggt cgagattttc cagtgcttcg accgcaggcg aagtttcttc
     3001 tggcactgac agcaaagaca tctgcgtacc atccacttgc gtcgcggcgg cgttcggcga
     3061 aatgctttcc agctcacgca gtttttgccg tgcgcgctta ataacctctt tcggtacgcc
     3121 cgccagagca gcaaccgcca gaccgtagct tttgctcgcc gcgccgtcct gcacgctgtg
     3181 cataaaggcg atagtgtcgc cgtgctccag cgcatcgaga tgtacgttgg cgacgccttc
     3241 cattttctcc ggtagctggg tcagctcgaa atagtgggtg gcaaacagcg tcaatgcctt
     3301 aatcttattc gccagatttt ctgcgcacgc ccacgccagc gacagaccat cgtaggtgga
     3361 cgttccacgc ccgatctcat ccattaacac cagactgtat tcggtggcgt tatgtaaaat
     3421 attggcggtt tcagtcatct ccaccataaa ggttgagcgc ccggaagcca ggtcatctgc
     3481 cgcgcctacg cgggtaaaga tgcgatcgat aggtccaatc tcgacttttt gtgccggtac
     3541 atagctgccg atgtaggcca tcagcgcaat cagtgcggtc tggcgcatat aggtactttt
     3601 accgcccatg ttcgggccgg taataatcaa catacggcgc tgcggcgaca gattcagcgg
     3661 gttagcgata aatggctcat tcagtacttg ttcaactacc ggatggcgac cttcggtaat
     3721 gcgaatgccc ggtttatcaa tgaaggtcgg gcaggtgtag ttcagggtat aggcccgttc
     3781 cgccaggtta accagcacgt cgagttccgc cagcgcgctc gcgctctgtt gcaacgcttc
     3841 cagatgcggc aacagcaggt cgaacagctc ttcataaagc tgtttttcca gtgccagtgc
     3901 tttgcctttt gaggtgagaa ctttatcttc gtactctttt agctctggaa tgatgtagcg
     3961 ctcggcgttt ttcagcgtct ggcgacgcat gtagttgatg ggtgccagat ggctttgccc
     4021 acggctgatt tgaatgtagt agccgtgcac cgcattaaag ccaactttta gcgtgtccag
     4081 gccggtacgt tcacgctcgc ggacttccag acgctccaga taatcggtcg cgccgtcagc
     4141 cagcgcgcgc cactcatcca gctcttcgtt atagcccgat gcgataacac caccgtcgcg
     4201 taccagcacc ggcggtgtgt cgatgattgc tcgctccagc agatcgcgca gctcggcaaa
     4261 ctcgcccatc ttctcacgta gcgcctgtac cggtgcacta tcgacagttt ctaactgcgc
     4321 acgcagctcc ggcagttgct ggaaagcgtg gcgcatacgg gccagatcgc gtgggcgagc
     4381 agttcgtaaa gccagacgtg ccagaatacg ttccaggtcg ccgacctgac gcagtaccgg
     4441 ctgcaactcg gcggtgaaat cctgcaatgc gccaatagtt tgctggcgct caagcaacac
     4501 gcgggtatcg cgcactggca tatgcagcca gcgtttcagc atacgactac ccatcggcgt
     4561 gacggtgcag tcgagcacag aagccagcgt attttccgca ccgcccgcca ggttctgagt
     4621 aatttccagg ttacgacgtg tcgcggcatc cataatgatg ctgtcctgct cacgttccat
     4681 ggttatagaa cgaatatgcg gcagggtcgt gcgttgggta tctttcgcat actgcaacag
     4741 acaaccggca gcacaaagtc cgcgcggcgc gttctcgacg ccaaaaccga ccagatcgcg
     4801 ggtgccaaat tgcagattca actgctggcg cgcggtgtcg atttcaaact cccacagcgg
     4861 gcgacggcgc aggccgcgac ggccttcaat cagcgacatc tcggcgaaat cttctgcata
     4921 cagcagttcc gccggattag agcgttgcag ctctgccgcc atcgtttcgc ggtcggccgg
     4981 ttcgctcagg cgaaaacgcc cggagctgat atccagcgtc gcgtagccga aacctttgct
     5041 gtcctgccag atagccgcca gcaggttgtc ctgacgctcc tgtaacaggg cttcatcgct
     5101 gatggtgcct ggcgtaacga tacgcacaac tttgcgctca accggacctt tgctggtcgc
     5161 cggatcgcca atttgttcgc agatggcaac ggactctccc tgattcacca gtttggcgag
     5221 atagttttcc accgcatggt agggaatccc cgccatcggg atcggctctc ccgccgaagc
     5281 accgcgtttg gtcagtgaaa tatccagcag ttgcgacgcg cgttttgcgt cgtcataaaa
     5341 cagttcataa aaatcaccca tccggtaaaa cagcaggatc tcgggatgct gggctttcag
     5401 cttgagatac tgctgcatca tgggcgtatg ggcgtcgaaa ttttctattg cactcatggg
     5461 gttatgtccg gttccctgat attaaatggg gtgtgatggt ttttattttc gttgtaatca
     5521 cataaggcgc acatgatacc ggagttaatc atttcagggg aagaagcgaa cggcgagttc
     5581 ttgatgctgg ttccgggaaa gatgctgttt tgcagctttt gcgtgatgct tatgaagcaa
     5641 gtacgcaaaa ttgtatcgat tggcgaaccc ttcttcttta gagtcatagt gccctttaag
     5701 at
//
LOCUS       DAEEGA010000173         5691 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEEGA010000173 DAEEGA010000000
VERSION     DAEEGA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.174"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(23..589)
                     /gene="satP"
                     /locus_tag="KLH46_003952"
     CDS             complement(23..589)
                     /gene="satP"
                     /locus_tag="KLH46_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBE0380435.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(738..1451)
                     /gene="msyB"
                     /locus_tag="KLH46_003953"
     CDS             complement(738..1451)
                     /gene="msyB"
                     /locus_tag="KLH46_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBE0380436.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(1477..1881)
                     /locus_tag="KLH46_003954"
     CDS             complement(1477..1881)
                     /locus_tag="KLH46_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBE0380437.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            2258..4174
                     /gene="dnaK"
                     /locus_tag="KLH46_003955"
     CDS             2258..4174
                     /gene="dnaK"
                     /locus_tag="KLH46_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBE0380438.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4263..5393
                     /gene="dnaJ"
                     /locus_tag="KLH46_003956"
     CDS             4263..5393
                     /gene="dnaJ"
                     /locus_tag="KLH46_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBE0380439.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(5497..5649)
                     /gene="mokC"
                     /locus_tag="KLH46_003957"
     CDS             complement(5497..5649)
                     /gene="mokC"
                     /locus_tag="KLH46_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBE0380440.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1496 a         1410 c         1495 g         1290 t
ORIGIN      
        1 gagcgggggg atctcaagac aattagtggg attcaccaat cggcagaacg gtgcgaccaa
       61 actgctcgtt cagcacttca cccatcgcca gatagattgc gctggcaccg cagatcagac
      121 caatccagcc ggcaaagtgg atgatcgcgg cattaccggc aatgttaccg atcgccagca
      181 gggcaaacag cacggtcagg ctaaagaaaa cgaattgcag gacgcgcgcg cctttcagag
      241 tgccgaagaa cataaacagc gtaaatacgc cccacagacc caggtagaca ccaaggaact
      301 gtgcatttgg cgcatcggtc agacccagtt tcggcatcag cagaatcgca accagcgtca
      361 gccagaaaga accgtaagag gtgaatgcgg ttaaaccgaa agtgttgcct tttttgtact
      421 ccagcagacc agcaaaaatt tgcgcgatgc cgccgtagaa aatgcccatg gcaagaataa
      481 taccgtccag agcgaaataa cccacgttgt gcaggttaag cagaatggtg gtcatgccga
      541 agcccatcag gcccagcggt gccggattag ccaacttagt gttgcccata attcctcaaa
      601 aatcatcatc gaatgaatgg tgaaataatt tccctgaata actgtagtgt tttcagggcg
      661 cggcataata atcagccagt ggggcagtgt ctacgatctt ttgaggggaa aatgaaaatt
      721 ttccccggtt tccggtatca gacctgagtg gcgctaacca tccggcgcag gcaggcgatt
      781 tgcagtacgg ctggaatcgt cacgcgatag gtgctgccgc tgaccgcttt aaccccattt
      841 aatgctgcgc ccacagggcc tcccagcccc gcgccgcgca gcaaaccatg cccaagtacg
      901 ctcattgctg cgtgggtgcg taaaatgcgg gtcagttggc tggaaagcaa atgcgacaca
      961 ccttttgcca ataatttgtc tttcatcagc agcggcagca gctcttccag ctcattcacc
     1021 ctggcatcga ccgcgtgcag aaactcctgc ttatgttcct cgtccatttt cttccaggta
     1081 ttacgcagaa attgttccag taactgttgc tcaatttcaa acgtagacat ctctttgtcg
     1141 gctttcagct tcaatcgctt tgaaacatcg agcaaaatgg cccgatacaa tttaccgtgt
     1201 ccgcgcagtt tgttggcgat actatcgcca ccaaaatgct gtaattctcc ggcaatcagc
     1261 tgccagttgc ggcgatgttg ctcgggatgc ccttccatcg atttaaacag ttcgttgcgc
     1321 atcagtatgc tggagaggcg agttttgcct ttttcattat gggtgagcaa tcgggcgaaa
     1381 tttgccaact gttcctcact acaatgctga agaaaatcca gatctgaatc attcaggtaa
     1441 ttaacattca ttttttgtgg cttctatatt ctggcgttag tcgtcgccga taattttcag
     1501 cgtggccata tccgatgagt tcaccgtatg accggaaaag gtgatttttg agacgcagcg
     1561 tttattgtcg ttatcgctgt taatgttgat ccagtcagtg gtttgccctt cttttatttc
     1621 tgaaggaata ttcaggctct gactggcgct acgggcggct ttgaaataaa ccgatgcacc
     1681 acttaactgt aaatcgccat ggtcggcaga gagttgtatg cgtttcacaa tgcgacaaac
     1741 aggaagtttc agcgccagat cgttggtttc gttacgcggc attgcaatga cgccgaggag
     1801 tttatggtcg tttgcctgcg ccgtgcagca cagcatcagg ctaatcgcca ggctggcgga
     1861 aatcgtaaaa acggatttca taaggattct cttagtggga agaggtaggg ggatgaatac
     1921 ccactagttt actgctgata aagagaagat tcaggcacgt aatcttttct ttttattaca
     1981 attttttgat gaacgccttg gctgcgattc attctttata tgaataaaat tgctgtcaat
     2041 tttacgtctt gtcctgccat atcgcgaaat ttctgcgcaa aagcacaaaa aatttttgca
     2101 tctccccctt gatgacgtgg gttacgaccc catttagtag tcaaccgcag tgagtgagtc
     2161 tgcaaaaaaa tgaaattggg cagttgaaac cagacgtttc gcccctatta cagactcaca
     2221 accacatgat gaccgaatat atagtggaga cgtttagatg ggtaaaataa ttggtatcga
     2281 cctgggtact accaactctt gtgtagcgat tatggatggc accattcctc gcgtgctgga
     2341 gaacgccgaa ggcgatcgca ccacgccttc tatcattgcc tatacccagg atggtgaaac
     2401 tctggttggt cagccggcta aacgtcaggc agtgacgaac ccgcaaaaca ccctgtttgc
     2461 gattaaacgc ctgattggcc gccgcttcca ggacgaagaa gtacagcgtg atgtttccat
     2521 catgccgttc aaaattattg ctgctgataa cggcgacgca tgggtcgaag ttaaaggcca
     2581 gaaaatggca ccgccgcaga tttctgctga agtgctgaaa aaaatgaaga aaaccgctga
     2641 agattacctg ggtgaaccgg taactgaagc tgttatcacc gtaccggcat actttaacga
     2701 tgctcagcgt caggcaacca aagacgcagg ccgtatcgct ggtctggaag taaaacgtat
     2761 catcaacgaa ccgaccgcag ctgcgttggc ttacggtctg gacaaaggca ctggcaaccg
     2821 tactatcgcg gtttatgacc tggggggtgg tactttcgat atttctatta tcgaaatcga
     2881 cgaagttgac ggcgaaaaaa ccttcgaagt tctggcaacc aacggtgata cccacctggg
     2941 tggtgaagac ttcgacagcc gtctgatcaa ctatctggtt gaagaattca agaaagatca
     3001 gggcattgac ctgcgcaacg atccgctggc aatgcagcgc ctgaaagaag cggcagaaaa
     3061 agcgaaaatc gaactgtctt ccgctcagca gaccgacgtt aacctgccat acatcactgc
     3121 agacgcgacc ggtccgaaac acatgaacat caaagtgact cgtgcgaaac tggaaagcct
     3181 ggttgaagat ctggtaaacc gttccattga gccgctgaaa gttgcactgc aggatgctgg
     3241 cctgtccgta tctgatatcg acgacgttat cctcgttggt ggtcagactc gtatgccaat
     3301 ggttcagaag aaagttgctg agttctttgg taaagagccg cgtaaagacg ttaacccgga
     3361 cgaagctgta gcaatcggtg ctgctgttca gggtggtgtt ctgactggcg acgtaaaaga
     3421 cgtactgctg ctggacgtta ccccgctgtc tctgggtatc gaaaccatgg gcggtgtgat
     3481 gacgacgctg atcgcgaaaa acaccactat cccgaccaag cacagccagg tgttctctac
     3541 cgctgaagac aaccagtctg cggtaaccat ccatgtgctg cagggtgaac gtaaacgtgc
     3601 ggctgataac aaatctctgg gtcagttcaa cctagatggt atcaacccgg caccgcgcgg
     3661 catgccgcag atcgaagtta ccttcgatat cgatgctgac ggtatcctgc acgtttccgc
     3721 gaaagataaa aacagcggta aagagcagaa gatcaccatc aaggcttctt ctggtctgaa
     3781 cgaagatgaa atccagaaaa tggtacgcga cgcagaagct aacgccgaag ctgaccgtaa
     3841 gtttgaagag ctggtacaga ctcgcaacca gggcgaccat ctgctgcaca gcacccgtaa
     3901 gcaggttgaa gaagcaggcg acaaactgcc ggctgacgac aaaactgcta tcgagtctgc
     3961 gctgactgca ctggaaactg ctctgaaagg tgaagacaaa gccgctatcg aagcgaaaat
     4021 gcaggaactg gcacaggttt cccagaaact gatggaaatc gcccagcagc aacatgccca
     4081 gcagcagact gccggtgctg atgcttctgc aaacaacgcg aaagatgacg atgttgtcga
     4141 cgctgaattt gaagaagtca aagacaaaaa ataatcgccc tataaacggg taattatact
     4201 gacacgggcg aaggggaatt tcctctccgc ccgtgcattc atctaggggc aatttaaaaa
     4261 agatggctaa gcaagattat tacgagattt taggcgtttc caaaacagcg gaagagcgtg
     4321 aaatcagaaa ggcctacaaa cgcctggcca tgaaatacca cccggaccgt aaccagggtg
     4381 acaaagaggc cgaggcgaaa tttaaagaga tcaaggaagc ttatgaagtt ctgaccgact
     4441 cgcaaaaacg tgcggcatac gatcagtatg gtcatgctgc gtttgagcaa ggaggcatgg
     4501 gcggcggtgg ttttggcggc ggcgcagact tcagcgatat ttttggtgac gttttcggcg
     4561 atatttttgg cggcggacgt ggtcgtcaac gtgcggcgcg cggtgctgat ttacgctata
     4621 acatggagct caccctcgaa gaagctgtac gtggcgtgac caaagagatc cgcattccga
     4681 ctctggaaga gtgtgacgtt tgccacggta gcggtgcaaa accaggtaca cagccgcaga
     4741 cctgtccgac ctgtcatggt tctggtcagg tgcagatgcg ccagggattc ttcgctgtac
     4801 agcagacctg tccacactgt cagggccgcg gtacgctgat caaagatccg tgcaacaaat
     4861 gtcatggtca tggtcgtgtt gagcgcagca aaacgctgtc cgttaaaatc ccggcagggg
     4921 tggacactgg agaccgcatc cgtcttgcgg gcgaaggtga agcgggcgag catggcgcac
     4981 cggcaggcga tctgtacgtt caggttcagg ttaaacagca cccgattttc gagcgtgaag
     5041 gcaacaacct gtattgcgaa gtcccgatca acttcgctat ggcggcgctg ggtggcgaaa
     5101 tcgaagtacc gacccttgat ggtcgcgtca aactgaaagt gcctggcgaa acccagaccg
     5161 gtaagctatt ccgtatgcgc ggtaaaggcg tcaagtctgt ccgcggtggc gcacagggtg
     5221 atttgctgtg ccgcgttgtc gtcgaaacac cggtaggcct gaacgaaaag cagaaacagc
     5281 tgctgcaaga gctgcaagaa agcttcggtg gcccaaccgg cgagcacaac agcccgcgct
     5341 caaagagctt ctttgatggt gtgaagaagt tttttgacga cctgacccgc taacctcccc
     5401 aaaagcctgc ccgtgggcag gcctgggtaa aaatagggtg cgttgaagat atgcgagcac
     5461 ctgtaaagtg gcggggatca ctccccgccg ttgctcttac tcggattcgt aagccgtgaa
     5521 aacagcaacc tccgtctggc cagttcggat gtgaacctca cagaggtctt ttctcgttac
     5581 cagcgccgcc actacggcgg tgatacagat gacgatcagg gcgacaatca tcgccttatg
     5641 ctgcttcatt gctctcttct ccttgacctt acggtcagta agaggcactc t
//
LOCUS       DAEEGA010000174         5683 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEEGA010000174 DAEEGA010000000
VERSION     DAEEGA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.175"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            872..3157
                     /gene="nrdA"
                     /locus_tag="KLH46_003958"
     CDS             872..3157
                     /gene="nrdA"
                     /locus_tag="KLH46_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBE0380441.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3391..4521
                     /gene="nrdB"
                     /locus_tag="KLH46_003959"
     CDS             3391..4521
                     /gene="nrdB"
                     /locus_tag="KLH46_003959"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBE0380442.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4521..4775
                     /gene="yfaE"
                     /locus_tag="KLH46_003960"
     CDS             4521..4775
                     /gene="yfaE"
                     /locus_tag="KLH46_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBE0380443.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="KLH46_003961"
     CDS             complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="KLH46_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBE0380444.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
BASE COUNT         1456 a         1488 c         1349 g         1390 t
ORIGIN      
        1 atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca actcgctttg
       61 atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga gaaaagagat
      121 gcaatcattg acgggagtag agataaatac tccttgcata gaaagataat ccgcatatta
      181 atctgaccat cattttatat aagacggcat atttaaacgc attatacaat atgggttatc
      241 tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt tgatgaataa
      301 tatcagtgcg tcataattca agttaataac cttaagggat accagttata tttaaactaa
      361 attaaagtaa tgaataattt tcttataata taaggtaaat taacaaaatg gcttcgcatt
      421 taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac attcgcttat
      481 atattgacca caactgatac atcagattat gtgatgactc gtgcttagat caatttttgc
      541 aatcattagc aaaaagatta ataaaccatc tatatcaatt tatccaacct attctgccgt
      601 tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg actttcccgg
      661 acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt tatccacaaa
      721 gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc aaacataccc
      781 cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg aaactattgc
      841 ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg tgacaaagcg
      901 cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg attgggcggc
      961 agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca ttcagtttta
     1021 tgacggtatc aagacctctg acatccacga aactattatc aaggctgccg cagacctgat
     1081 ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct tccacctgcg
     1141 taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg tgaaaatggt
     1201 cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag agttcaagca
     1261 gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg ccgttaagca
     1321 gctggaaggc aaatatctag tacagaaccg cgtgaccggc gaaatctacg aaagcgccca
     1381 gttcctttat attcttgttg ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct
     1441 gcaatacgtg aagcgttttt atgacgcggt ttccacattt aaaatttcgc tgccgacgcc
     1501 aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg
     1561 cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgtgaaat atgtttccca
     1621 gcgtgccggg atcggcatca acgccggacg tattcgtgcg ctgggcagcc cgattcgcgg
     1681 tggcgaagcg ttccataccg gctgtatccc gttctacaaa catttccaga cagcggtgaa
     1741 atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca
     1801 tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggggttgaag gcaaccgcgt
     1861 gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgcc tgctgaaagg
     1921 tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg cgttcttcgc
     1981 cgatcaggaa gagtttgaac gcctgtatac caaatatgag aaagacgaca gcatccgcaa
     2041 gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg cgtctaccgg
     2101 tcgtatctat attcagaacg ttgaccactg taatacccat agtccgtttg atccgaccat
     2161 cgcgccagtt cgccagtcta acctgtgcct ggaaattgcc ctgccgacca aaccgctgaa
     2221 cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg
     2281 cgcaattaat agcctggatg aactggaaga gctggcgatt ctggcggttc gtgcacttga
     2341 cgcgctactg gattatcagg attacccgat cccggccgcc aaacgtggag cgatggggcg
     2401 tcgtacgctg ggtattggtg tgattaactt cgcttactat ctggcgaagc acggtaaacg
     2461 ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcga ttcagtatta
     2521 cctgctgaaa gcgtctaatg agctggctaa agagcaaggc gcgtgcccgt ggtttaacga
     2581 aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc tggatgccat
     2641 cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca aaacgcacgg
     2701 cctgcgtaac tccacgcttt ctgccctgat gccgtccgag acctcttcgc agatctctaa
     2761 cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt cgaaagacgg
     2821 tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg agctgctgtg
     2881 ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga aatttatcga
     2941 tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag gaaaagtgcc
     3001 gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca aaacgctgta
     3061 ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac cgtcaatcca
     3121 ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg gatgcggcgt
     3181 aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc cagcgtcgca
     3241 tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc tcggatttgt
     3301 aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa aatgccttat
     3361 ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc acagacgaaa
     3421 aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc tcgctacgat
     3481 cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt cttctggcgt
     3541 ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc ggagcacgaa
     3601 aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat tcagggtcgt
     3661 agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac ctgggtcgaa
     3721 acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat tcgtaatatc
     3781 gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat ccagaaacgt
     3841 gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta ctggcacctt
     3901 ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct gcgcgagctg
     3961 aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat ccgcttctac
     4021 gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg taacgccaag
     4081 attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca gcatatgctg
     4141 aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga agaatgtaag
     4201 caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg ggcggattat
     4261 ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca gtacgttgaa
     4321 tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca gacgcgctcc
     4381 aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt tgctccgcag
     4441 gaagtggaag tcagttctta tctggtcggg cagattgacg cagaagtgga caccgacgat
     4501 ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc acacaactgc
     4561 tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat gtggcggttg
     4621 agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc gcaggtcagg
     4681 tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg ccctgttgtt
     4741 gccgggcaaa aggcgatatt gaaatcgagt tgtgatcggg tactagcgcc agatacgacg
     4801 cttacgcgta ttatctggcc tacaacgatt acatggcgta gtaatacgct ttcacctgct
     4861 cccagtccgc tttagggatc ggctccagat atttttccag ctggcggaag tcatgattaa
     4921 tcgctttatc gcgacgcaag cggcgacgac ttttctccag atcaagaaaa ccagcttctg
     4981 ccttaccttc tgttttcaca tagatatggc gaacatagca acagccatgc tgacgattaa
     5041 cgctatgcat tttcttaaac gccagtgcca ctgctttcaa catggcttgc cgtacttcgt
     5101 cagaataagg cgatactgca tgccgggcat accagtcagc aatgctgatg aaccccgcca
     5161 tatcttcagt caccagcaac gcgcgccatt caccctcaat tttcaccgct tcaccaaaaa
     5221 cgatcttcgg cacgatgaca ccggcccgtt ccagttcttt aattaccgca acctcacgga
     5281 caatcgttgg tcggccgaac ggataacgta ctgaatgaaa cagatgatgc gtcatgcgct
     5341 ttacatacag ctttttgccg ttgcgctcga cgcattgcac cccgctcata ccattacggc
     5401 gatagttagg ctcttcaacc cagtcgccct ctgttgccca ccagtgatta aattcgtcgt
     5461 actttgctga aactgccata ccctatcgcc tgtcattttt attaacgaca atgactatag
     5521 gtggttacct gaggaaaatc ttaatgaaac gtgtcgtatt aatgaatttc ttttgccgct
     5581 acaggtgtga caccgctttt gcccttcatt tgcagcgcat agattccacc agccactacc
     5641 actaatagtg cgcccagcag ataaaatgtg aatttaatca gtc
//
LOCUS       DAEEGA010000175         5648 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEEGA010000175 DAEEGA010000000
VERSION     DAEEGA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.176"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..212
                     /locus_tag="KLH46_003962"
     CDS             <1..212
                     /locus_tag="KLH46_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HBE0380445.1"
                     /translation="SVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLRE
                     VIEKLNDWPSNQVHELLPWNFSSVK"
     gene            245..742
                     /locus_tag="KLH46_003963"
                     /pseudo
     CDS             245..742
                     /locus_tag="KLH46_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
     gene            764..2755
                     /gene="cyoB"
                     /locus_tag="KLH46_003964"
                     /pseudo
     CDS             764..2755
                     /gene="cyoB"
                     /locus_tag="KLH46_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2745..3359
                     /locus_tag="KLH46_003965"
     CDS             2745..3359
                     /locus_tag="KLH46_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBE0380446.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3359..3688
                     /locus_tag="KLH46_003966"
     CDS             3359..3688
                     /locus_tag="KLH46_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBE0380447.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3700..4590
                     /gene="cyoE"
                     /locus_tag="KLH46_003967"
     CDS             3700..4590
                     /gene="cyoE"
                     /locus_tag="KLH46_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBE0380448.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVTSGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4775..5086
                     /locus_tag="KLH46_003968"
     CDS             4775..5086
                     /locus_tag="KLH46_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBE0380449.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            5070..5228
                     /locus_tag="KLH46_003969"
     CDS             5070..5228
                     /locus_tag="KLH46_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBE0380450.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            5221..5619
                     /locus_tag="KLH46_003970"
     CDS             5221..5619
                     /locus_tag="KLH46_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0380451.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1245 a         1398 c         1495 g         1510 t
ORIGIN      
        1 gatcggtggc ggttggaaga aaaaattatc tctttttcgg ctcagacaag ggaggagaaa
       61 gtgcggcgat catctacagt ctgctggtca cctgcaaaca gaacgaagtg gagccggagg
      121 actggttgcg cgaagtgatc gagaagctca atgactggcc gtcgaaccaa gtgcatgaac
      181 tgctgccctg gaacttctcg tctgtaaaat aatccttacg ctacgtactt ctcggggcgc
      241 ttacaatgaa atcgctttcc cggcgaacac tccggtgtac ttcaaagtga cctccaactc
      301 cgtgatgaac tccttcttca ttccgcgtct gggtagccag atttatgcca tggccggtat
      361 gcagactcgc ctgcatctga tcgccaacga acccggtact tatgacggta tctccgccag
      421 ctacagcggg ccgggcttct caggcatgaa gttcaaagct attgcaacac cggatcgcgc
      481 cgaattcgac caatgggtcg caaaagcgaa acagtcgccg aataccatgt ctgacatggc
      541 tgcgttcgaa aaactggccg cgcctagcga atacaaccag gtggaatatt tctccaacgt
      601 gaaaccagac ttgtttgccg atgtgattaa caagtttatg gctcacggta agagcatgga
      661 catgacccag ccagaaggtg agcacagtgc acacgaaggt atggaaggca tggacatgag
      721 ccacgcggaa tccgcccatt aaaggggttg aggaagaata aagatgttcg gaaaattatc
      781 acttgatgca gtcccgttcc atgaaactat cgtcatggtt acgatcgctg gcattatttt
      841 gggaggtctg gcgctcgttg gcctgatcac ttacttcggt aagtggacct acctgtggaa
      901 agagtggctg acctccgtcg accataaacg cctcggtatc atgtatatca tcgtggcgat
      961 tgtgatgttg ctgcgtggtt ttgctgacgc cattatgatg cgtagccagc aggctcttgc
     1021 ctcggcgggt gaagcgggct tcctgccacc tcaccactac gatcagatct tcaccgcgca
     1081 cggcgtgatt atgatcttct tcgtggcgat gcctttcgtt atcggtctga tgaacctggt
     1141 ggttccgctg cagatcggcg cgcgtgacgt tgcgttcccg ttcctcaaca acttaagctt
     1201 ctggtttact gttgttggcg tgattctggt taacgtttct ctcggcgtgg gcgaatttgc
     1261 gcagaccggc tggctggcct atccaccgct atcgggaata gagtacagtc cgggagtcgg
     1321 tgttgattac tggatatgga gtctccagct atccggtata ggtacgacgc ttaccggtat
     1381 caacttcttc gttaccattc tgaagatgcg cgcaccgggc atgaccatgt tcaagatacc
     1441 agtatttacc taggcatcac tgtgcgcgaa cgtactgatt attgcttcct tcccaattct
     1501 gacggttacc gtcgcgttgt tgaccctgga tcgctatctg ggcacccatt tctttaccaa
     1561 cgatatgggt ggcaacatga tgatgtacat caacctgatt tgggcctggg gccacccgga
     1621 agtttacatc ctgatcctgc ctgttttcgg tgtgttctcc gaaattgcgg caaccttctc
     1681 gcgtaaacgt ctgtttggtt atacctcgct ggtatgggca accgtctgca tcaccgtgct
     1741 gtcgttcatc gtttggctgc accacttctt tacgatgggt gcgggcgcga acgtaaacgc
     1801 cttctttggt atcaccacaa tgattatcgc catcccgacc ggggtgaaga tcttcaactg
     1861 gctgttcacc atgtatcagg gccgcatcgt gttccattct gcgatgctgt ggactatcgg
     1921 ttttatcgtc accttctcgg tgggcggtat gactggcgtg ctgctggcag taccgggcgc
     1981 ggacttcgtt ctgcataaca gcctgttcct gattgcgcac ttccataacg tgatcatcgg
     2041 cggcgtggtc ttcggctgct tcgcagggat gacctactgg tggcctaaag cgttcggttt
     2101 caaactgaac gaaacctggg gtaaacgcgc gttctggttc tggatcatcg gcttcttcgt
     2161 tgcctttatg ccgctgtatg cgctgggctt catgggcatg acccgtcgtt tgagccagca
     2221 gattgacccg cagttccaca ccatgctgat gattgcagcc agcggtgcag tactgattgc
     2281 gctgggtatt ctctgcctcg ttattcagat gtacgtttct attcgcgacc gcgaccagaa
     2341 ccgtgacctg actggcgacc cgtggggtgg ccgtacgctg gagtgggcaa cctcttcccc
     2401 gcctccgttc tataactttg ccgtagtgcc gcacgttcac gaacgtgatg cattctggga
     2461 aatgaaagag aaaggcgaag cgtataaaaa gcctgaccac tatgaagaaa ttcatatgcc
     2521 gaaaaacagc ggtgcaggta tcgtcattgc agctttctcc accatcttcg gtttcgccat
     2581 gatctggcat atctggtggc tggcgattgt tggcttcgca ggcatgatca tcacctggat
     2641 cgtgaaaagc ttcgacgagg acgtggatta ctacgtgccg gtggcagaaa tcgaaaaact
     2701 ggaaaaccag catttcgatg agattactaa ggcagggctg aaaaatggca actgatactt
     2761 tgacgcacgc gactgcccac gcgcacgaac acgggcacca cgatgcaggc ggaaccaaaa
     2821 tcttcggatt ttggatctac ctgatgagcg actgcattct gttctcaatc ttgtttgcta
     2881 cctatgccgt tctggtgaac ggcaccgcag gcggcccgac aggtaaggac attttcgaac
     2941 tgccgttcgt tctggttgaa actttcttgc tgttgttcag ctccatcacc tacggcatgg
     3001 cggctatcgc catgtacaaa aacaacaaaa gccaggttat ctcctggctg gcgttgacct
     3061 ggttgtttgg tgccggattt atcgggatgg aaatctatga attccatcac ctgattgtta
     3121 acggcatggg gccggatcgc agcggcttcc tgtcagcgtt ctttgcgctg gtcggcacgc
     3181 acggtctgca cgtcacttct ggtcttatct ggatggcggt gctgatggtg caaatcgccc
     3241 gtcgcggcct gaccagcact aaccgtaccc gcatcatgtg cctgagcctg ttctggcact
     3301 tcctggatgt ggtttggatc tgtgtgttca ctgttgttta tctgatgggg gcgatgtaat
     3361 gagtcattct accgatcaca gcggcgcgtc ccatggcagc gtaaaaacct acatgacagg
     3421 ctttatcctg tcgatcattc tgacggtgat tccgttctgg atggtgatga caggggctgc
     3481 ctctccggcc gtaattctgg gaacaatcct ggcaatggca gtggtacagg ttctggtgca
     3541 tctggtgtgc ttcctgcaca tgaataccaa atcagatgaa ggctggaaca tgacggcgtt
     3601 tgtcttcacc gtgctaatca tcgctatcct ggttgtaggc tccatctgga ttatgtggaa
     3661 cctcaactac aacatgatga tgcactaaga gcggcggtta tgatgtttaa gcaatacctg
     3721 caagtaacga aaccaggcat catctttggc aacctgatct cggtgattgg gggattcctg
     3781 ctggcctcaa agggcagcat tgattatccc ctgtttatct acacgctggt tggggtgtca
     3841 ctggttgtga cgtcgggttg tgtgtttaac aactacatcg acagggatat cgacagaaag
     3901 atggaaagga cgaagaatcg ggtgctggtt aaaggcctga tctctcctgc tgtctcgctg
     3961 gtgtacgcca ccttgctggg tattgctggc tttatgctgc tgtggtttgg cgcgaatccg
     4021 ctggcctgct ggctgggggt gatgggcttt gtggtttatg tcggcgtcta tagcctgtac
     4081 atgaaacgcc actcagtcta cggcacattg attggttcgc tctccggcgc tgcgccgtcg
     4141 gtgattggct actgtgcagt aaccggcgag tttgatagcg gcgcagcgat cctgctggct
     4201 atcttcagcc tgtggcagat gcctcactcc tatgccatcg ccattttccg ctttaaggat
     4261 taccaggcgg caaacattcc ggtattgcca gtggtaaaag gcatttcggt ggcgaagaat
     4321 cacatcacgc tgtatatcat cgcctttgcc gttgccacgc tgatgctctc tcttggcggt
     4381 tacgctgggt ataaatatct ggtggtcgcc gcggcggtta gcgtctggtg gttaggtatg
     4441 gctctgcgcg gttataaagt tgctgatgac agaatctggg cgcgcaagct gttcggcttc
     4501 tctatcatcg ccatcactgc cctctcggtg atgatgtccg ttgattttat ggtaccggac
     4561 tcgcatacgc tgctggctgc tgtgtggtaa caaaacctct ctattaaaaa ggtgctacgg
     4621 cacctttttt attgtctgag atcagagata cggctaataa ccgtatctct tgacagagtg
     4681 agcagatgca cataaattaa tcaaacggtt gatagccgta ttatgtatga gatcgactcc
     4741 ctacgatttc attcagttaa tcaaagaagt cataatgcct ggaaaaacag ccgctctata
     4801 cgatgtcgat aaaacgctga aaaatgcgcg ggtcgaactc aaaactagtc ccgacgccaa
     4861 aaacaaactt cgtgaagctg cgcaagccgt tggggtagat ttaagcgctt ttattttgag
     4921 cgctgctatg gaacgcgccg aaagtgtgct tgataaccaa cgtcgccgtg agctttcgaa
     4981 tcaaagctgg gaactgatga accaactcat cgctgaacct gctcaaccga cgctcgccct
     5041 caaggcgtta atgaaaagga aaaacagcga tggtcgacaa gcatgaagag attactctgc
     5101 ccatagtcct ctcctgtaat tatcagtctg atattactta tcctgggcaa aaacagtttg
     5161 attgcggtaa ccctgttatc gataaatttg tacgcgctac gcgcatcgct aaagaaaagt
     5221 gtgcgtaata gcgactgtgc ggctaaagca cttattgaca gacaaagtgg tgaactgatt
     5281 ggcatctgta cttttacggc atattcgctg gaaagacaac gcgtttctgg cgtccttcag
     5341 ggttcacaac cttcagaaat tggtgttgtc agattagtca tgttgggggg agcgcggaag
     5401 tatcaaaagc ggggctttgg tcaggaccta ctatgtgatt tttttgaaca tgtaaaaaaa
     5461 attcacccgg cattaccaat taaaggggtt tatcttgatg ctgaccctgc cgccattaac
     5521 ttttatgctc gtctcggctt tgttcagctt tcagcgacac caaatgcttt tggtgctgta
     5581 cctatgtttt tggcgattca gcatattctc gcggcttaga ttattatcgc cacccgccag
     5641 ggcaagtt
//
LOCUS       DAEEGA010000176         5642 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEEGA010000176 DAEEGA010000000
VERSION     DAEEGA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.177"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            263..1189
                     /gene="tauA"
                     /locus_tag="KLH46_003971"
     CDS             263..1189
                     /gene="tauA"
                     /locus_tag="KLH46_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0380452.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKQGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1202..1969
                     /gene="tauB"
                     /locus_tag="KLH46_003972"
     CDS             1202..1969
                     /gene="tauB"
                     /locus_tag="KLH46_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBE0380453.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1966..2793
                     /gene="tauC"
                     /locus_tag="KLH46_003973"
     CDS             1966..2793
                     /gene="tauC"
                     /locus_tag="KLH46_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBE0380454.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2790..3641
                     /gene="tauD"
                     /locus_tag="KLH46_003974"
     CDS             2790..3641
                     /gene="tauD"
                     /locus_tag="KLH46_003974"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HBE0380455.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3747..4721)
                     /gene="hemB"
                     /locus_tag="KLH46_003975"
     CDS             complement(3747..4721)
                     /gene="hemB"
                     /locus_tag="KLH46_003975"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBE0380456.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5245..5613
                     /locus_tag="KLH46_003976"
                     /pseudo
     CDS             5245..5613
                     /locus_tag="KLH46_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1326 a         1410 c         1529 g         1377 t
ORIGIN      
        1 attgacctgc ctttagggtg cctttaaaat tcgtttttaa aggctacgat gacatttttg
       61 aagagtctta aaagaattaa aatacacggc gcgcctatct tgaatgtttt atcgatatcg
      121 aagcattgag catttttaac tcgttttatc ttgaatgcaa tcggcgcata acttattgaa
      181 tcccatcaaa tcccgtcttt ttttacccat tcccggttat cttgaataaa ttacctagtc
      241 atataacaca cttcttgccg cactggcatt catcgctttt caggcacagg cggtgaacgt
      301 caccgtggcg tatcaaacct cagccgaacc ggcgaaagtg gctcaggccg acaacacctt
      361 tgctaaagag agcggggcga gcgtggactg gcgtaagttt gacagcggag ccagcatcgt
      421 gcgggcgctg gcttctggcg acgtgcaaat cggcaacctc ggttccagcc cattagcggt
      481 tgcagccagc caacaggtgc cgattgaagt cttcttgctg gcgtcaaaac agggtaactc
      541 cgaagcgctg gtggtaaaga aaactatcag caaacccgaa gatttgattg gtaaacgcat
      601 tgccgtaccg tttatctcca ccacccacta cagcctgctg gcggcgctga aacactgggg
      661 gattaaacca gggctagtgg agattgtgaa cctgcagccg cccgcgatta tcgccgcatg
      721 gcagcggggt gatattgatg gtgcttatgt ctgggcaccg gcggttaacg ccctggaaaa
      781 agacggcaag gtgctgaccg attctgaaca ggtcgggcag tggggtgcgc caacgctgga
      841 cgtctgggtg gtgcgcaaag attttgccga gaaacatcct gaggtcgtga aagcgttcgc
      901 caaaagcgcc atcgatgcac agcaaccgta cattgctaac cctgacgcgt ggctgaaaca
      961 gccggaaaac atcagcaaac tggcgcgttt aagcggcgtg cctgaaggtg acgttccggg
     1021 gctggtgaag ggaaatacct atctgacgcc gcagcaacaa acggcagaac tgaccggacc
     1081 ggtgaacaaa gcaatcatcg acaccgcgca gtttttgaaa gagcagggca aggtcccggc
     1141 tgtagcgaat gattacagcc agtacgttac ctcgcgcttc gtgcaataaa aggaggcgca
     1201 gatgctgcaa atctctcatc tctatgccga ttatggcggc aaaccggcac tggaagatat
     1261 caacctgacg ctggaaagcg gcgagctact ggtggtgctg gggccgtccg gctgcggtaa
     1321 aaccaccctg ctgaatctga ttgccggttt tgtgccttat cagcatggca gcattcaact
     1381 ggcgggtaag cgtattgagg gaccgggagc agagcgtggc gtagtttttc agaatgaagg
     1441 gctgctaccg tggcgcaatg tacaggacaa cgtggcgttc ggcctgcaat tggcaggtat
     1501 agagaaaatg cagcgactgg aaatcgcgca ccagatgctg aaaaaagtgg ggctggaagg
     1561 cgcagaaaaa cgctatatct ggcagctttc cgatggtcaa cgtcagcggg tggggattgc
     1621 ccgcgcgctg gcggcgaatc cccagctgtt attactcgac gaaccgtttg gtgcgctgga
     1681 cgccttcacc cgcgaccaga tgcaaactct gctgctgaaa ctctggcagg agacgggcaa
     1741 gcaggtgctg ttgattaccc acgatataga agaagcggtg tttatggcga ctgaactggt
     1801 tctgctttca tccggccctg gccgtgtgct ggagcggctg cggctcaact ttgctcgccg
     1861 ctttgttgcg ggagagtcga gccgcagcat caagtccgat ccacaattca tcgccatgcg
     1921 cgaatatgtt ttaagccgcg tatttgagca acgggaggcg ttctcatgag tgtgctcatt
     1981 aatgaaaaac tgcattcgcg gcggctgaaa tggcgctggc cgctctcgcg tcaggtgacc
     2041 ttaagcattg gcacgttagc ggttttactc actgtatggt ggacggtggc ggcgctgcaa
     2101 ctgattagcc cgctgttttt gccgccgccg caacaagtac tggcaaaact gctcaccatt
     2161 gccggaccgc agggatttat ggacgccacg ctgtggcagc atctggcagc cagcctgacg
     2221 cgcattgtgc tggcgctact cgcggcggtg gtgatcggta ttccggtcgg gatcgccatg
     2281 gggctaagcc cgacggtgcg cggcattctg gatccggtaa tcgaacttta tcgtccggtg
     2341 ccaccgctgg cttatttgcc gctgatggtg atctggtttg gtattggtga aacctcgaag
     2401 atcttactga tctatttagc gatttttgcg ccggtggcga tgtcggcgct ggcaggggtg
     2461 aaaagcgcgc agcaggttcg cattcgtgcc gcccagtcgc tgggtgccag ccgtgcgcag
     2521 gtgctgtggt ttgtcatttt gcccggcgcg ctgccggaaa tcctcaccgg attacgcatt
     2581 ggtctgggtg tgggctggtc tacgctggta gcggcggagc tgattgccgc gacacgcggt
     2641 ttaggattta tggttcagtc agcgggtgaa tttctcgcga ctgacgtggt actggcgggg
     2701 atcgcggtga ttgcgattat cgcctttctt ttagaactgg gtctgcgcgc gttacagcgc
     2761 cgtctgacgc cctggcatgg agaagtacaa tgagtgaacg tctgagcatt accccgctgg
     2821 ggccgtatat cggcgcacaa atttcgggtg ccgacctgac gcgcccgtta agcgataatc
     2881 agtttgaaca gctttaccat gcggtgctgc gccatcaggt ggtatttctg cgcgatcaag
     2941 ctattacgcc gcagcagcaa cgcgcgctgg cccagcgttt tggcgaactg catattcacc
     3001 ctgtttaccc tcatgccgaa ggggttgacg agatcatcgt gctggatacc cataacgata
     3061 atccgccgga taacgacaac tggcataccg atgtgacatt tattgaaacg ccacccgcag
     3121 gggcgattct ggcagctaaa gagttacctt cgaccggcgg tgatacgctc tggaccagcg
     3181 gtattgcggc ctatgaggcg ctctctgttc ccttccgcca gctgctgagc tggctgcgtg
     3241 cggagcatga tttccgtaaa tcattcccgg aatacaaata ccgcaaaacc gaggaggaac
     3301 atcaacgctg gcgcgaggcg gtcgcgaaaa atcctccgtt gctacatccg gtggtgcgaa
     3361 cgcatccggt gagcggtaaa caggcgctgt ttgtgaatga aggctttact acgcgaattg
     3421 tcgatgtcag cgagaaagag agcgaagcct tgttaggctt tttattcgcc catatcacta
     3481 aaccggagtt tcaggtgcgt tggcgctggc aaccgaatga tattgcgatt tgggataacc
     3541 gcgtgactca gcactatgcc aatgccgatt acctgccaca gcggcggatt atgcatcggg
     3601 cgacgatcct tggggataaa ccgttttatc gggcggggtg atacgagtgc ggacggttcc
     3661 ctcgccccct tggggagagg gttagggtga gggggcgttc actgtacttt caacaggtta
     3721 actccccctt tctgagagga aaaaaattaa cgcagaatct tcttctcagc caaatccagc
     3781 gcaaagtagc tgaaaatcag atccgcaccc gcacgcttaa ttgaacctaa gctttcgagc
     3841 acgactttct cttcatctat agcacccgcc agcgcggcga acttaatcat cgcgtactca
     3901 ccgctcacct gatacgcgcc aatcggcaat tcagtacgtt cacgcagctc acgcacgatg
     3961 tcgaggtacg ctccagcagg tttaaccatc aggcagtctg cgccctgggc ttcatccagc
     4021 agtgactcac gaatcgcctc gcgacggttc attgggttca tctgatagct tttgcggtcg
     4081 ccttttaatg cgcttcctgc agcttcacgg aacgggccat aaaaggaaga ggcgaacttg
     4141 gtcgaatacg acataatcgc cgtatcttta aagcctgcag cgtccagcgc ctgacgaatc
     4201 gcctgtacct ggccgtccat cgcggcggaa ggggcgatga agtctgcgcc tgcagcagct
     4261 gcaaccacgg cttgcttgcc taaattttcc agagtcgcgt cgttgtcgac gccatgctcg
     4321 cacagcacac cgcagtgacc gtgagaagtg tattcacaga aacaagtgtc tgacatgacg
     4381 atcatttctg gcacagtctg cttgcagatg cgcgacatac gcgccaccag tccatcttcc
     4441 cgccaggcgt cgctgccggt ttcatcggta tggtgagaga tgccaaaagt cattacggaa
     4501 cggataccgg cgttggcgat gcgttcaatt tcgcgtgcca gatgtttctc tggaatgcgc
     4561 atcacgcctg gcatggcttc aacggctttg tagtcgtcaa tttcttcttc aacaaagatc
     4621 ggcaacacca ggtcgttaag gctaagtgtt gtctcttcaa acatagcgcg cagcgcagga
     4681 gatttgcgca ggcgacgagg gcgttggatt aagtctgtca tggtctgcct gatgtttgtg
     4741 gaattaaggt gcacagtata cctgaagcgg ggcaggagtg ttttacgaaa gttgtcgcga
     4801 tgttgacaag aagagaatga aagagagacc aggacatctt tgtaatagaa atagcaataa
     4861 tttcatattc actgaaaata tttttaatct ttatttatag ctcttggtta ttattttttg
     4921 gcgtttggtt gtgctgctat aaattgaata attcaaactt tgttgcatat tgataagata
     4981 tttatctggt ttgtgttttt gatatttata acgcgtacgc atcaaaaata acattatctg
     5041 caacatactg aaatatcgtc cttttatcaa tttgatgcgt gtttttctcc ataaattata
     5101 tatgtccaca ttcggactta ggggaaagaa taattgaacc attcgtctgt aacgcagcat
     5161 aatcgttagc gcgaaacata atatgttttc tatgcattga taattgatgg atcaacttat
     5221 tacgtccctg aggagggatg acaaatgcac tcctggaaaa agaaacttgt agtatcacaa
     5281 ttagcattgg cttgcactct ggctatcacc tctcaggcta atgcagcgac caacgatatt
     5341 tctggtcaaa cttacaatac tttccatcac tacaacgacg ccacctatgc tgatgacgtt
     5401 tactatgatg gttatgtagg ctggaacaac tatgccgctg atagctatta caacggcgat
     5461 atctacccgg tcattaataa cgctaccgtt aacggcgtga tttctaccta ctatctggac
     5521 gacggtattt ctaccaatac caacgccaat agtctgacaa tcaaaaacag cactattcac
     5581 ggtatgattt attccgagtg catgacaact gattagactg gccccctgaa tctccagaca
     5641 ac
//
LOCUS       DAEEGA010000177         5600 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEEGA010000177 DAEEGA010000000
VERSION     DAEEGA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.178"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..411
                     /locus_tag="KLH46_003977"
     CDS             <1..411
                     /locus_tag="KLH46_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0380457.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="KLH46_003978"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="KLH46_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="KLH46_003979"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="KLH46_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HBE0380458.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1380 a         1402 c         1330 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tagtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggagg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc
//
LOCUS       DAEEGA010000178         5578 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEEGA010000178 DAEEGA010000000
VERSION     DAEEGA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.179"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(1..279)
                     /locus_tag="KLH46_003980"
     CDS             complement(1..279)
                     /locus_tag="KLH46_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380459.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="KLH46_003981"
     CDS             220..405
                     /locus_tag="KLH46_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380460.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="KLH46_003982"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="KLH46_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBE0380461.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="KLH46_003983"
     CDS             complement(1254..1670)
                     /locus_tag="KLH46_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380462.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="KLH46_003984"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="KLH46_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2877
                     /locus_tag="KLH46_003985"
     CDS             1837..2877
                     /locus_tag="KLH46_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0380463.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSKCDFCH"
     gene            complement(2905..2994)
                     /locus_tag="KLH46_003986"
     tRNA            complement(2905..2994)
                     /locus_tag="KLH46_003986"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2958..2960),aa:Ser,seq:cga)
     gene            3088..3885
                     /gene="mtfA"
                     /locus_tag="KLH46_003987"
     CDS             3088..3885
                     /gene="mtfA"
                     /locus_tag="KLH46_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBE0380464.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYVASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3986..4061
                     /locus_tag="KLH46_003988"
     tRNA            3986..4061
                     /locus_tag="KLH46_003988"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4019..4021,aa:Asn,seq:gtt)
     gene            4223..5485
                     /locus_tag="KLH46_003989"
                     /pseudo
     CDS             4223..5485
                     /locus_tag="KLH46_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1504 a         1321 c         1292 g         1461 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt gtgatttttg tcactgactt
     2881 aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc ccggtggagt
     2941 tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct ctccgttcag
     3001 atggttgtca tgatgccagg aaatttggca ttttaacagt ccctgtccgt gcaattttgt
     3061 tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt acaagaatca
     3121 gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt aacgtgtctg
     3181 acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca gcaaaaacgg
     3241 cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat agcacttcta
     3301 ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca tgaagtctta
     3361 atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg tctggtgcat
     3421 aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt tttgaactgg
     3481 ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca tgaagtcgct
     3541 cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat ttcgttgcgt
     3601 gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca ggaagaaatc
     3661 gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ttgccagtga tcctgctgaa
     3721 tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc tcctcgtttc
     3781 ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca gagactgcat
     3841 cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac aactttgaag
     3901 attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag gtaccgctaa
     3961 gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac ggcggactgt
     4021 taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt tttcatgcat
     4081 ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc ttctcggtgc
     4141 attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga tcaggttagt
     4201 tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca ccctcaagcc
     4261 ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg tcaagccggg
     4321 tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc gcattgcgct
     4381 gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag gtatccgtaa
     4441 aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg gctcacgaac
     4501 accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca ggaaatggtc
     4561 gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc cggtcatcgg
     4621 gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga aagggatcga
     4681 ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta acataatgcg
     4741 ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg gcggcgtgac
     4801 cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc ctgaactgct
     4861 tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg ttctgctgat
     4921 gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag agattgattt
     4981 cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg tgcgttattc
     5041 cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac agtccatcgc
     5101 cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc ccggcgacca
     5161 taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg tgatgggtta
     5221 cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca gtgcgctgat
     5281 ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc aggagcgcaa
     5341 taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca aagcgatgat
     5401 gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc cttatacaat
     5461 tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg caaattacca
     5521 ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg aaaaacac
//
LOCUS       DAEEGA010000179         5559 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEEGA010000179 DAEEGA010000000
VERSION     DAEEGA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.180"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..641
                     /locus_tag="KLH46_003990"
     CDS             <1..641
                     /locus_tag="KLH46_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HBE0380465.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="KLH46_003991"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="KLH46_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBE0380466.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="KLH46_003992"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="KLH46_003992"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBE0380467.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1379 a         1359 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatag
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagctatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaaaa
//
LOCUS       DAEEGA010000180         5530 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEEGA010000180 DAEEGA010000000
VERSION     DAEEGA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.181"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..162
                     /locus_tag="KLH46_003993"
     CDS             <1..162
                     /locus_tag="KLH46_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0380468.1"
                     /translation="HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIG
                     HYLNIKHYQ"
     gene            complement(255..737)
                     /locus_tag="KLH46_003994"
     CDS             complement(255..737)
                     /locus_tag="KLH46_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380469.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(805..2754)
                     /locus_tag="KLH46_003995"
     CDS             complement(805..2754)
                     /locus_tag="KLH46_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBE0380470.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2839..3174)
                     /locus_tag="KLH46_003996"
     CDS             complement(2839..3174)
                     /locus_tag="KLH46_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HBE0380471.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(3174..3530)
                     /locus_tag="KLH46_003997"
     CDS             complement(3174..3530)
                     /locus_tag="KLH46_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBE0380472.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3530..5026)
                     /locus_tag="KLH46_003998"
     CDS             complement(3530..5026)
                     /locus_tag="KLH46_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBE0380473.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(5023..5184)
                     /locus_tag="KLH46_003999"
     CDS             complement(5023..5184)
                     /locus_tag="KLH46_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HBE0380474.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5194..>5530)
                     /locus_tag="KLH46_004000"
     CDS             complement(5194..>5530)
                     /locus_tag="KLH46_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380475.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1332 a         1601 c         1193 g         1404 t
ORIGIN      
        1 cacgttatca gcaagcgtta cactcagcgc attgagcgac ataatctgaa tctgagacaa
       61 catctggcaa ggctgggacg gaagtcactg tcgttctcaa aatcggtgga gctgcatgac
      121 aaggtcatcg ggcattatct gaacataaaa cactatcagt aagttggagt cattaccaag
      181 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
      241 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      301 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      361 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      421 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      481 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      541 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      601 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      661 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      721 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      781 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      841 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      901 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      961 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
     1021 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
     1081 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
     1141 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1201 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1261 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1321 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1381 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1441 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1501 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1561 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1621 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1681 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1741 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1801 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1861 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1921 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1981 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     2041 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     2101 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     2161 ggccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2221 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2281 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2341 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2401 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2461 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2521 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2581 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2641 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2701 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2761 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2821 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2881 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2941 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     3001 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     3061 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     3121 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3181 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3241 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3301 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3361 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3421 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3481 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3541 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3601 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3661 gatactgttt aaacagatcg taattttcca caataccggc acgctccatc tgacgatatg
     3721 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3781 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3841 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3901 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3961 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     4021 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     4081 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     4141 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4201 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4261 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4321 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4381 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4441 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4501 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4561 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4621 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4681 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4741 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4801 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4861 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4921 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4981 acggcaccag cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt
     5041 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     5101 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     5161 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5221 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5281 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5341 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5401 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5461 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt
     5521 ttcatcacgc
//
LOCUS       DAEEGA010000181         5339 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEEGA010000181 DAEEGA010000000
VERSION     DAEEGA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.182"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="KLH46_004001"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="KLH46_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBE0380476.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="KLH46_004002"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="KLH46_004002"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBE0380477.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRSGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="KLH46_004003"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="KLH46_004003"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBE0380478.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="KLH46_004004"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="KLH46_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBE0380479.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1456 g         1283 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtt cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DAEEGA010000182         5289 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEEGA010000182 DAEEGA010000000
VERSION     DAEEGA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.183"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            437..2089
                     /locus_tag="KLH46_004005"
     CDS             437..2089
                     /locus_tag="KLH46_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBE0380480.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDPNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="KLH46_004006"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="KLH46_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="KLH46_004007"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="KLH46_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3526)
                     /locus_tag="KLH46_004008"
                     /pseudo
     CDS             complement(2596..3526)
                     /locus_tag="KLH46_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(3809..4009)
                     /locus_tag="KLH46_004009"
                     /pseudo
     CDS             complement(3809..4009)
                     /locus_tag="KLH46_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            4387..5136
                     /locus_tag="KLH46_004010"
     CDS             4387..5136
                     /locus_tag="KLH46_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBE0380481.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT         1458 a         1190 c         1183 g         1458 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggtacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccctaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgg
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gagcagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaaagaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgtaccat ttttcagcgt tccccagaag ctttccatcg
     3841 gagcgttgtc gtaacagtta cctttacgcg acattgatgt tttcagacca aactgctcct
     3901 gtatgacccg gtaatcgtat gcgcagtact gtgaacctcg atcggagtgg tggattagcc
     3961 cggcaggtgg gcgctggctc ctgagcacca taaacagggc tttacccgta aatattggct
     4021 ccttctgatg ccattctatt tcaggaagga gtgtccgtta aactcaggct acctcaaaaa
     4081 gttatcggtg cgtttatcga aaaacatatg ttctactaat tggagtcatt gtctgtaaaa
     4141 gcaactcaaa aatacaagtt gcttattccc cttgttggta agaacttaat tctaactgtt
     4201 tgttctgggt gttttaaacg tcctcctttt tatgcttgtt cataacggat atttaatagg
     4261 taacctttgt tttagcatct tctgatttat ttaaattatg tgatagcaca cacaatttta
     4321 ttatccagta ctttatactc ttaataaaat aattataaat atttatcctc tataaggaga
     4381 tgttatatga aaagacagct ttttactctt agtattgtcg gggtattttc tttaaatacc
     4441 tttgcatcat tccctccggg aaatgatgtg acaacaaaac ctgaccttta ctacctgaca
     4501 aacgataatg ctattgacag tctggcatta ttaccgcctc caccacaaat cggaagtatt
     4561 gccttcctga atgaccaggc catgtatgaa aaggggcgtt tattgcgaaa tactgaaaga
     4621 gggaagctgg cagctgagga tgccaatctc agtagtggtg gcgtggcaaa tgtattttct
     4681 gctgcttttg gttctccgat aacggctaaa gattcaccag aattgcataa attactgaca
     4741 aatatgattg aggatgccgg agacctggca acgcgttcag caaaagaata ttatatgcgt
     4801 attcgacctt ttgcttttta tggtgtttct acgtgtaata caaaagaaca ggatacatta
     4861 tccagaaatg gctcttatcc atcaggtcat acatcgattg gttgggcaac agcacttgta
     4921 ttgtccgaaa taaatccggc acgccaggat accattctga aacggggtta tgaactgggg
     4981 gacagcaggg ttatttgcgg ttatcactgg caaagtgatg ttgacgcagc acgtattgtt
     5041 ggctctgcta ttgtagcaac actgcactca aatcctgtgt tccaggcaca attacaaaaa
     5101 gcgaaagatg aattcgctaa caatcagaaa aaataatgct tttagccagt acgccttata
     5161 accccgacct tcagggctaa tgccagtcag ttaagcaact gactggctct ttttcagggc
     5221 tgtggggtat ttccagggcc tctcctttac cactctcgga aaggcccttt cccttcttgt
     5281 cggtaattt
//
LOCUS       DAEEGA010000183         5230 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEEGA010000183 DAEEGA010000000
VERSION     DAEEGA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.184"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(106..1815)
                     /locus_tag="KLH46_004011"
     CDS             complement(106..1815)
                     /locus_tag="KLH46_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HBE0380482.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2247..2966)
                     /locus_tag="KLH46_004012"
     CDS             complement(2247..2966)
                     /locus_tag="KLH46_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HBE0380483.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3091..3647)
                     /locus_tag="KLH46_004013"
                     /pseudo
     CDS             complement(3091..3647)
                     /locus_tag="KLH46_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3631..3889
                     /locus_tag="KLH46_004014"
                     /pseudo
     CDS             3631..3889
                     /locus_tag="KLH46_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(3998..4576)
                     /locus_tag="KLH46_004015"
     CDS             complement(3998..4576)
                     /locus_tag="KLH46_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380484.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(4997..5230)
                     /locus_tag="KLH46_004016"
                     /pseudo
     CDS             complement(4997..5230)
                     /locus_tag="KLH46_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1481 a         1113 c          908 g         1728 t
ORIGIN      
        1 ttgccaattg aggtggttgc ctgaaagttg gcagttaccc ggtcactaca ccttgttgga
       61 gtgaacatat caaaaacagg taattacaca atttaaatta cagtctcata aaaaatgatt
      121 aaatctattt attatttcaa taaacgtttt tttcatcttc tttgaaaatt gttctgctat
      181 agtatccggt tccagattgt tctcttttat tattctacaa tactctaata ttgtttcttt
      241 gtctctattt actacagata caaatacacc aggaatacca tctttattag ttgcggacag
      301 catttcgacc aatcgtcttg tggtaaaact atagtttttt agaatattac aaaaggcacg
      361 tattgtttcg gtatggccat tataaaatgc cataaaaagt cctgatgtac cgttgggatt
      421 ttttatccat aataattcat ctatatattc ctggtgaggg gggagtattt ttagtatatc
      481 tccatacgcc ataatggcct ttgtgtgccc attttgaagt gccataaaca acccatgctt
      541 tactttcaat aattctttaa tttgttcact actaagcatt ttcttattaa gcagacttcc
      601 gaacatacga atactatctg catgtccatt ctgcaatgca aaaaataacc caggtaaatc
      661 atggctgtct ttcgcagcta atagatataa tctcttatcg gaagttaacg gggttttaaa
      721 gactatttcc ccataggctt ttattgtgtc tacatgtccg ttcataaaag acatgcataa
      781 acccggcaca ttgtttgccc ctaccgcagc aagcaatgta agtattgttt cttgactaag
      841 attgcacata tttaatatct taccatatac ttgaataatg tctgaatatc cgttttgaag
      901 tgctatataa aatgcaggag taccatcaga gcttctcgtg gccagaaggt tcaccagttt
      961 agattgagaa atttcaccat ttttgtattt ttccatcagt ataaataata cttctgtcag
     1021 attctttgtt atcgcatgat atataactct ggaattgatc aattcatctg gtaaaagttt
     1081 cactgtgttt gttagagggg aattatgcct atcttcaaat attacctgcc cgggttgctc
     1141 cagaccataa attttccaat aaaaatcatc aaaaaaatcc ttagcggata attgactcag
     1201 ttcgaaatgg ctatcacaag taaatacagt acgtttatga gtaacagttc gattaggatc
     1261 gaaaaactga acaacccatt tttcactttc ttgtacgcgc tttattttca accctaacgc
     1321 catttggtgg tttaatgtgc atgctgataa tgttctgaca aagtctccat tttttttcat
     1381 gttataaaaa aaattagtaa taactgttcc ccacttttta ttttccacaa aataaatata
     1441 agctggtgat tttgaaaaat cttccgtttt ttccaaacat acattttttg caatagcatc
     1501 aggggtgcta agaagctgat agtttacctt cccatttgtt tccataaaat tcatttccca
     1561 ataggaagct aggtgacggc aaactatttg tcttccatat atatcttttg ccactccatt
     1621 tatatttacc tccacatttc tcttttctga aaaataaggt gctgttttat tcttaaactg
     1681 aattagttca ctggagtttg agaccctcat aatagaactt gttattgttc tattatctct
     1741 gttaacagac atatctgttg ataacgaatt ttttgttgaa aatatactag atgaaaatgt
     1801 tttatttaac ggcatagaag acaaccatag agatgttata atactattag attgcagtta
     1861 ctaatagtaa aagcctcctt acaaggaggt agcttattca gaatgctcct ataagatagc
     1921 gagctcgtca acgatacaga tgatctgatt atattgtgca aaaggagaac aggaaaatag
     1981 gcactggaaa agatgaaggc ctgatgggag cattaactaa tgcaaaatcc gacaggttaa
     2041 tatcccgaaa agcaatcaaa cgactcaaac aggagatgca cgctgaaatg gtggacaact
     2101 gttcggattt cagatgagta gctcagcagt ttatgagccg aatgctctgt cacgggcagg
     2161 aatcaggctg catacgcagt gccaaatcta atcgtagtta ttctaaccag ttttcaagct
     2221 acaaggtggt gtagctggca ttttctctac tctatcatca aacgataaaa tggttcctct
     2281 cgtaacattt gctcttgcct ttcacttcca tctcgatcgc ttcgtagttc attacgatag
     2341 ctgacatatt tccagtcttc aggtctaaca tctgacgccg gatactcccc gggagcgatt
     2401 ttgcttctta atagattaga ctcgagacac attataaatt gtcgtatttt atgaagtaat
     2461 aaagcactat attgcgagca ttcctgatct gattttacat atagcgtaaa ctgagctcct
     2521 atccccacac gagattgctg agaaactcga ttcatatcag ttatcttcca tttatctata
     2581 ggactgtcct ctgaaaataa taaaccagac aaaatttgga atgccagagg aacttgctcc
     2641 ctagctatac taatatgaaa cttgtcccca acaaaatccc ccttactctg aggattttca
     2701 cgacatgcat gaataaacac atctccacta tggttcacaa tctggaatcc ttggtacatg
     2761 acagaaaaag attgacatat atcagagtaa gttggcacgt tcgatgctac tttgaaatag
     2821 tacgctgggt attgccgtat ttggttgtac aatatattga agttattttt caatctttca
     2881 ttcgcagaaa gcatctgcgg tatttcagac ctcacaacat tcaaagaatc taaatttagt
     2941 tttagacagg gcttttttat gggcatagaa aacgtcctct ataaaataga tatattatct
     3001 tatattgatt gtttatcatc ttttataaaa cgatacatta tatgtaatgt atgtaagcgt
     3061 aagggtcctc tccgatcatg gtgggaaggc ttagaacgcc atattcagtt tcccatagtg
     3121 gaacatcact cccagcttaa agcgctcccg gttgcggtag cccttggctt ttattctcag
     3181 cagcctgatc ttgctgttca gtgcttccgc atttccgttt gatacgccat ggcgcatggc
     3241 gttcaggata ccgtacaacc ttttccctat cgttttagcc acattcttca tcatggggac
     3301 gtcactgttg gccgcagcca tctctgccag tcgttgcgtc tttcctcgct ccatggtctg
     3361 ctccagatat cctttgccag ttctttcagc gcccagcaca ggctcgtcag cttcatctgc
     3421 gcacgcagcc atatcagctt ttcctgcctg gattcggtca tccatttatc gctgtgctgc
     3481 cacaggaagc gagttccctt tgcctgacga cgactttcaa caggaagatg cggatgttca
     3541 ttctggcggg ttttatccac cacctcaccc ggttgtttcg ccacatgaaa gcgatcgaag
     3601 gctattttct caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt gctgagccag
     3661 tttagcattg ccttctacat gaccgatgcc tgagccggta taccgaactt tattgtcctc
     3721 aaccagtcat gttataagca agaaatgcac tcagcggata gagcgacata atctgaacct
     3781 gcggacacat cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt ctgatgatat
     3841 gcactacaag atcatcggct ggtatctgac aatcaaccac caccactaaa tctgcgtcac
     3901 gacctgcaac tgagtctggt tgaacaggct gaggccctgc tgaagcaacg cgaacctctc
     3961 gactcttgtt ggattttacc aaatagtttt tctaaaatta aacacattta tataagttct
     4021 ttgctgaaca aaacgcagcc cgccatcctt tttcattcat aaattcagca atggcagata
     4081 aaactttttc atcaagcata taaaaaataa tgccagtaga ctcaatatgc ctcatacaaa
     4141 taaggccgca catatccgta atatatttgt caatgctgct accatttgca tggtaatatg
     4201 tcatgctatt atctgatgat tttacatatg cagtatgaca tatttttcct tcacgattaa
     4261 caaaactgac aaaaccactt tcaccaggag taatatataa agatgaattt aacgtgcgct
     4321 cactaatatt cattaattgt tgaaattcaa tggaatagtt ttcagttaat gtatctatta
     4381 tcttggcttt gtctttcttt gttatctcat tgtctttttc ggcaagaaaa attgccactt
     4441 tatgacatgc attcagctcg ttaattttac ctgataaaac tgcttttctt accacatcgt
     4501 tgaactcatc gatagtgtgt tttttattat atatatcatg aaaaatatag cctaaagact
     4561 taaacgagac tttcatttta tattctcctt aaatattaac aggatatata tatcacattt
     4621 tcctctatat tttcagaaac cagctatcat tttccccatt actggtacgt cgcttcagat
     4681 atttccgcag caagcgataa ccctgcgcac tatgaggatg ccattcatta taatgtttga
     4741 acaccttcgc gagactactt tactgctgtt aacacgtcag gttcgaacat aacactgatt
     4801 taatcatatt tcatcgtttt gacgaagttt tctgccattc cattttgcac cgccgtgtgt
     4861 ttgggggcaa ggccgaccat ctgtgtttca ttcgccagat ataagtgatg agatggcatt
     4921 ctccctcctt cacggcagaa gcattttgac cgtttagggg atgcggacga ttttcatgcc
     4981 tgctaagata ctttcgttac tgatgctgtc cgttttccac catttcatca tcactgattg
     5041 gcgttgtgtt tttggtcagc actccagctt tacgtttctc ttttaaccgg taactctctc
     5101 ctttgatatt cagcgtgtag cagccgatct aggatcgctg ttgccagcac gtgatctccg
     5161 aacatttctc cccagtctgc gacccctttg tttgacgtca gtatgatgct cgctttttca
     5221 tatcgacagt
//
LOCUS       DAEEGA010000184         5164 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEEGA010000184 DAEEGA010000000
VERSION     DAEEGA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.185"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..221
                     /locus_tag="KLH46_004017"
     CDS             <1..221
                     /locus_tag="KLH46_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0380485.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(393..722)
                     /locus_tag="KLH46_004018"
     CDS             complement(393..722)
                     /locus_tag="KLH46_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBE0380486.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(727..912)
                     /locus_tag="KLH46_004019"
     CDS             complement(727..912)
                     /locus_tag="KLH46_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBE0380487.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(909..3548)
                     /locus_tag="KLH46_004020"
     CDS             complement(909..3548)
                     /locus_tag="KLH46_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBE0380488.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3756..4745
                     /gene="ldhA"
                     /locus_tag="KLH46_004021"
     CDS             3756..4745
                     /gene="ldhA"
                     /locus_tag="KLH46_004021"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBE0380489.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4856..>5164
                     /gene="hslJ"
                     /locus_tag="KLH46_004022"
     CDS             4856..>5164
                     /gene="hslJ"
                     /locus_tag="KLH46_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBE0380490.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1305 a         1323 c         1205 g         1331 t
ORIGIN      
        1 gtttgtaccg aatgtttgcc gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc
       61 gtctcgattt ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca
      121 tcggctttca ttggggattt tctgaccaga gtcatttttc aacggtattt aagcaacgct
      181 ttgggatgac gccaggcgag tatcgacgta aattccgcta actaattggc gttgcatgta
      241 ctgcaacgcc aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa
      301 tttgacagga gtttttgaat attcaccttg tttcaggaaa atagatcaat cgctttctcc
      361 tgtagcgtta tcccgatatt gcggttttgc tattaaaact ttcgcaccca tttaccgttt
      421 atcccttgta catattcccc cggtttggcc cgagctacga gcttttgccc ggcgagtttc
      481 gcgatatcat ctacagacac attattttgc tttgcccgtt gttggtaact cgctttacgt
      541 tcggcattaa tttcggttac taatttctct gtttcagcat ccgttttcag cgcaaccaga
      601 taaccataaa atgtttcacc tacccgcccc tgagttctgg cttcatccag agtcaatgcc
      661 attacattgc tgcttaccag cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc
      721 atcgcctcag aaaagatcgc tacgagtttc aagcagctct tcgacatctt tgtctgcctt
      781 gatgatgatc tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttaggtgc
      841 agcgacttca atgcgaggcg tacagccaac cagcataaaa gatgacgtca acgcagccag
      901 taaaattttc attgtttttc ctcacactct tttccttgcg gacagtcatt tcccggcaga
      961 cgtgcgttct gctccaacca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc
     1021 gtaaaaatat tctcttcatg atggtaatta aggtttaccg taccgctttt accatcaacg
     1081 cgactggtac ctgtaatgtt ggcctgcatg gttaataaac cgagattatc taagttaatt
     1141 tttgtcgatg accggctaat ttccatatag cgcaaccagt taattgctga acccgcagtc
     1201 atattgtctt tcaccaccgc atctgcggta tctttatcca gccgcaaggt cattggcccg
     1261 ctatttgcca gccaaccatc tttcactatc catttttcgt tgtttagcca taacggcaat
     1321 gcaccactaa atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg
     1381 ctggatgaga gattattcag ccgcaacagc gccgggtcat gttgcggcat acgtaattgt
     1441 ttcatcagca cgttaccgcc taacacatcg acgctaacat cagtcaacag caagggttct
     1501 tcttcagtcc acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga
     1561 ttaatcactt cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca
     1621 tcggcaaaac ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag
     1681 gcactaccgc ctttcaacac gccgtgccct cccgcgcgga atccttgttc aggcgcagca
     1741 gaaaatgcaa cctgagcata cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc
     1801 accagcggct ggaatacagt cagtgattgt tttggccacc aggcgttacc gcgcagacga
     1861 ataccgtccc agcgaccatt tacccgaacc gggccaatct caccagcatg taaatcgcct
     1921 ttaaagagga aataggtcgg attgcgccca tcgacgctaa attttaaggt tgatggcggt
     1981 aacacactgc cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag
     2041 gagggatgct gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt
     2101 tctaccgtca tcgtaccgta ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta
     2161 atcgtgctgt catgccactc accttttcct gcgacatccc aggtggcatt catcggtgta
     2221 aaactccctt ttccccagta gcgccactgc cagcggccag cgtcagggag aaaatcattc
     2281 gccagcccat ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg
     2341 gcctgcaaac gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg
     2401 atttcatcga tgtccagcga atcgatgact cttccctttg aacgaagtaa cgcgcctggc
     2461 tcaaaggtca gcgttgggtc agacagactt ccacttagct gcgcaggtaa acgggcatat
     2521 aaaatgaggt ccgcctgttt cgcttcaccg gtcagctgca gaggcagttg actgttatcc
     2581 atgcttaatt ttcctgggcc aaaattaagc accgcgttgc ccttacccgc ttgcccctgg
     2641 gtcagcacat tcagtcgtcc gctgaccaga gcgttctcaa gccctgcctg ccagttgtcg
     2701 actttgacac ccagtcggcc actcaaagga aaacctgcat acagccagct ccagcgacca
     2761 tcgcttacgg tcaattgttg acgagtaatt tgccacggca aatcgagcaa cggatcgccg
     2821 ttatcccgtg ccagcacgat caattgcccg ctattttcct gccagtccag ctcggcatcc
     2881 accagtgacg gttcctgcgg caagtttaac gtcgcagtag catgcccact tacaggaagt
     2941 ccatccggca cgagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca
     3001 aacgcgacga catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat
     3061 ttaacttttt cgccctgata acgcagttgc tggatatcag aggttaatgc gagagagagt
     3121 tttccctgcc attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta
     3181 ggcagcatgg actgccactg cgcgagggtt tttggagcag ctggcgattg ctccgtctgc
     3241 ggcaatttcg ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg
     3301 ctgggatgtg aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa
     3361 cggagatcgg gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata
     3421 cgtgtcccga gcggtagcca gatgtccgcc agcgtgggaa cccacagccc gagcgtcatc
     3481 agcagcgtca acggcacaag aataatcagt aataacagcg cgagaacggc tttatattta
     3541 cccagcatgg gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt
     3601 gtggcatgtt taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa
     3661 tttttcaata tcgccatggc tttcaattat atttgaaatt ttgtaaaata tttttagtag
     3721 cttaaatgtg attcaacatc actggagaaa gtcttatgaa actcgccgtt tatagcacaa
     3781 aacagtacga caagaagtac ctgcaacagg tgaacgagtc ctttggcttt gagctggaat
     3841 tttttgactt tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat
     3901 gtattttcgt aaacgatgac ggcagccacc cggtgctgga agagctgaaa aagcacggcg
     3961 ttaaatatat cgccctgcgc tgtgccggtt tcaataacgt cgaccttgat gcggcaaaag
     4021 aactggggct gaaagtagtc cgtgttccag cctatgatcc agaggccgtt gctgaacacg
     4081 ccatcggtat gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg
     4141 acgctaactt ctctctggaa ggtctgaccg gctttactat gtatggcaaa acggcaggcg
     4201 ttatcggtac cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc
     4261 gtctgctggc gttcgatccg tatccaagtg cagcggcgct ggaactcggt gtggagtatg
     4321 tcgatctgcc aaccctgttc tctgaatcag acgttatctc tctgcactgc ccgctgacac
     4381 cggaaaacta ccatctgttg aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga
     4441 tcgtcaatac cagtcgcggt gcattgattg attctcaggc ggcaattgaa gcgctgaaaa
     4501 atcagaaaat tggttcgttg ggtatggacg tctatgagaa cgaacgcgat ctgttctttg
     4561 aagataaatc caacgacgtg atccaggatg acgtattccg tcgcctgtct gcctgccaca
     4621 acgtgctgtt taccgggcac caggcattcc tgacagcaga agctctgacc agtatttctc
     4681 agactacgct gcaaaactta agcaatctgg aaaaaggtga aacctgcccg aacgaactgg
     4741 tttaatcttg cagctcccct gcaatccagg ggagctgatt cagataatcc ccaatgacct
     4801 ttcattctct attcttaaaa tagtcctgag tcagaaactg taattgagaa ccacaatgaa
     4861 gaaagtagcc gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa
     4921 aattgctgtt acgccagaac agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg
     4981 taagcccgtg accagcgata aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat
     5041 ttccggcagc atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac
     5101 agccaaaggg ctggcaatga cccgtatgat gtgcgctaac ccgcagctta atgaactcga
     5161 taac
//
LOCUS       DAEEGA010000185         5149 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEEGA010000185 DAEEGA010000000
VERSION     DAEEGA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.186"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            188..445
                     /locus_tag="KLH46_004023"
                     /pseudo
     CDS             188..445
                     /locus_tag="KLH46_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(495..1445)
                     /gene="uspE"
                     /locus_tag="KLH46_004024"
     CDS             complement(495..1445)
                     /gene="uspE"
                     /locus_tag="KLH46_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBE0380491.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTTMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="KLH46_004025"
     CDS             complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="KLH46_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBE0380492.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="KLH46_004026"
     CDS             complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="KLH46_004026"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBE0380493.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3070..3213)
                     /locus_tag="KLH46_004027"
                     /pseudo
     CDS             complement(3070..3213)
                     /locus_tag="KLH46_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3603..4418)
                     /locus_tag="KLH46_004028"
     CDS             complement(3603..4418)
                     /locus_tag="KLH46_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0380494.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4515..>5149
                     /locus_tag="KLH46_004029"
     CDS             4515..>5149
                     /locus_tag="KLH46_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE0380495.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1375 a         1173 c         1225 g         1376 t
ORIGIN      
        1 tattttaccg cgacaattca ttcagaacat caatagtcag ggaaggaagt agcaacatta
       61 gctaactaat caatgcgttg attgtaaatc cagctaagag gtgaggtttt cagagcagac
      121 aacggtgaaa cgtcatggta ttgttacgtt taggtaacaa gaaatttgtc tgcacaagga
      181 ttacatcatg attatggcga aactgaagtc agcgaaaggg aagaaatttc tctttggttt
      241 gttggcggtt ttcattattg cggcgtcggt tgtgactcgc gcgaccatcg gcggcgttat
      301 agaacagtac aatattccgc tgtctgagtg gacgacatca atgtatgtga tttagtcatc
      361 gatgattttt gtttatagcc tggtctttac tgtgttgctg gcaatcccgt tgggaattta
      421 tttccttggc ggcgaagagc agtaagtaaa aaataggccc gataactcgg gccttgtcag
      481 ttattgaaga gtcgttaatc gtcttcttcg tcatccagtt caacgggtgt ctgatactgg
      541 tcaggtttaa tgaccagcag gtcgcagcga agatgatcaa tcacctgttc cgccgtgttg
      601 ccgaggaatg ctgctgaaat accggtgcgt ccgaccgtgc ccagaaccac aattcccgcc
      661 tgtaaatgct ccgccaaatc aggaatcacc tcttcgggca gacctttttc tacgtgcgtc
      721 atgttttcat taatgccgaa tttctgccgc agggctttca ttgccagcaa atgttgccca
      781 cgaatggcat cgttataaac gctcgggtca aattccggca gttcaatcgc gatattaatt
      841 ggcgttaccg gataagcgcc aaccagatga acttcggtat ggttgacttg ttctgccagt
      901 tcgatcgtct ctttgaccag tttttcattg agcgcattat gatacggctc ttcactggcg
      961 agattcaccg ccaccagcgc cttgcctcct tccggccacg gctggtcttt caccatccac
     1021 accgggcttg ggcatttgcg taacagatgc cagtccgttg gcgtaaaaat caccgcttcc
     1081 agacggtcat gttggtgcgc catttttagc accaaatcgt gtccgccgct gatcacttcc
     1141 tgaatgatgg cttcgaaagg acggttatgc cagaccactt taatttcaat gggaacgcca
     1201 gcattgagat aatattttgc ctgctcgtgg atccaggctg tacgctggct gatgacgccc
     1261 tgacgcatag tggtacgttc gtccggggag agcagggtgg tcatttcgta tgagaagtca
     1321 tagatcggca aaaaggcttt aattttgcca ccaatccgtt gatgtaaata aacagctcgc
     1381 cgcaatgctg gttggtcgtc ctggttagga tcgataacaa cgagcatgtt ctgatacata
     1441 gccatacagg gtctccttac aacaactgtc aacgcagttt gtaattaaaa gattaaccca
     1501 tatctggtga atgaaacagt gatgaacctt ctgccagatc aataaatcag aaaaatttaa
     1561 tgatatgaca gaaggatagt gagttatgcg gaaaaatcag gcaacgttac gcgtatgacc
     1621 agcaagctgg gccagcgcat cgttattttc gatggtgatg tatttacctt tgactgccag
     1681 cataccgctt ttctggaagc gacccagcag acggctgatg gtttctaccg tcaggcccag
     1741 atagttacca atatcgccac gagtcatcgt caggcggaat tcacgagggg agaagccgcg
     1801 ttgggcaaaa cgacgggaca ggttgtagat gaatgcagcc agacgttcct cggcattttt
     1861 cttcgacaac agcaagatca tgtcctgatc gcctttgatt tcaccgctca tcagacgcat
     1921 catctgctga cgcagattcg gcattttacc ggacaaatcg tccagcgttt cgaacggaat
     1981 ttcacatacc atcgaggttt ccagcgcctg agcgaagctc gggtgatggc cgctgccgat
     2041 ggcgtcaaat cccactaggt cgcctgctaa atggaaacca gtgatttgct cgtcgccttg
     2101 ctcagtgatt gtataacttt taatcgtacc ggagcggatg gcataaagcg atttaagttc
     2161 atcaccagcc ttaaacagcg tctggccttt ctgaataggc ttcttccgct caatgatatt
     2221 atcaagctga tcaagctcat gttcgttgag tgtgaacggg atgcaaagct ggctgatgct
     2281 gcaatcctgg caatggatag cacaaccgcc agactgaatg cgccgtataa ttcgcttttc
     2341 cgggatcata ggtctgctca agccgtaatt gatatttgtc aattttaaca tctttttagg
     2401 gagcaagtaa gtctaagcaa accttaacag cagagaattc cgatattaga tgtaaatata
     2461 tgtctatcta tttgaaactc cttaagttgt caagggtaac tttacataaa agtgtgaaca
     2521 agctggcaca aattgtttaa tgtttacagc aaaagataac cttcgtggcg caataaccac
     2581 tcttttcgct gaactccgcc tgcatatccg gtcatggtgc cgtttcggcc aataacccga
     2641 tggcaaggta cgacgatgct gatgggattc gatccgtttg ccgcaccaac ggcacgcgcc
     2701 gcgccagggc ggcccaattg ttcagccagt tggccgtaat gcattacctg cccgcagggg
     2761 atagtgcgta gtgttttcca gacttcgcgc tgaaatggcg tcccccccgt ggcagtggga
     2821 agcgtatcaa taatgctaag attaccggca aaatattcac gaagcttgtc gcttaaacca
     2881 cctgggttgg tggcagaaat gcgctcatag ccttctttgc gataatggat gtccagcagc
     2941 tgcaccatgc gttcgctgta ctcttcccat tcaaccgccc gcaggcgaaa ttgctcatcg
     3001 caaatcaccc acagtggacc cagtggcgtg gcaatttttt cttcaagtaa tctcagcatc
     3061 cgttctctct taagacaaac gtgggtaaat acccggacct atcggcagac cgacaagata
     3121 ccacgccagc aacatcagca gccataccac caaaaagata agtggatagg gcaagactaa
     3181 cgaatagtaa gtacccagtt tcgcgtctgg tttgtcgcag tctgatagca taaagcgatt
     3241 ttttaatttt tctgcctgaa gaaatgaggg caagccatca catggatcga gggtgggata
     3301 attccagtca gtgaaatttt accgtaggct atctgttatt tgattctgtg gcgtgatagc
     3361 cgccagacac cactgaactc gtcactcatt ccccaatccc ggcgagaatg tggttattta
     3421 cagagctaaa aaatgaataa aaaaatagag cctgtcgaca tccgccagac tctacagtac
     3481 acacagcagt gcatcctcgt cttaaatccg gcctggggtc ctggtgaccg gtgacagtgt
     3541 tataacggtt ttacccatcc gttgccaatt ttcttcagtg acagatttca cgaaaatata
     3601 agttaaatca tttgtcttgt gattgcataa ttatgcatcg ttaatcgacg agtaaattta
     3661 ccgggcgagt agcccgttcg acgcttaaac atattgccga acgattcagc tgaggcatag
     3721 cccagacgat gggcaatttt ggtcaacgaa tatccatcat caagaagcgt cactgcagaa
     3781 ataacgtgca tttgctgaac ccagtggcga aatgttaatc ccgtctcttt aacaaataag
     3841 cgcatgagtg tacggctact gacgttaatt ttatccgcta gattatccag tgttggcaga
     3901 tacccttggt tgtttagcaa attctcacaa agctgctgta gccgctcatc atttggccaa
     3961 gggagttcaa gtgacatttt cacccccatt tttatttcct gaagtatcag tgtctcgagg
     4021 gctgaaaaac gtaactgttg tttatctgtg gtactttgcg acggtggaag ttggttaatt
     4081 gcaattagta attcccttaa caggggtgaa atacccgtca tgaagcaaac tgttcccagc
     4141 gtttcacttt ccgcttctga aaccagtacg gtttttaaaa gtacgttgcc tgtcgcaaca
     4201 aacttatgcg gcatttgtgg gggcagccaa agcgcgctgc tggcatggac tagccagacg
     4261 ctttctccag cccatacacg aacgactcct tcagccgggt aaagcaactg ggcatagaga
     4321 tgtttatgcc agggaactgt acctccattc agatgttccg aatccagtcc gataatgtga
     4381 tgccctggtg atgctttggg ttgcagtgta attgtcattt acagtaggta ttttgtcaga
     4441 aatcatcaaa taagccatgt taaattttaa atatgacgta tgcaagtttc cggatatata
     4501 cggtgtaaag agtgatgata atttctaaaa aacagcttat tggggtggta gcgatcggta
     4561 ttcttttgac tggcgttgtc ttttttatct ggagggtaag caaaggaaga tttatacaga
     4621 cgacggatga tgcttacatt ggtggtaata tcaccacggt ggcaagtaaa gtttctggtt
     4681 atatttctgc tatagaggtg cgcgataatc agagcgtaaa aaaaggcgat attatcctgc
     4741 gtctcgatga tcgggattat cgtgcaaatg tcgccaggct tgaggcaaaa ataaaaagta
     4801 gcaaagccaa tctcgaaggc attcaagcca ctattacgat gcagcagtcg ataattcaaa
     4861 gtgccagtga aacatggcag gcagtaaaac atgaggagca aaagcgcctg cgagatactg
     4921 aacgttatga aaaactcgca caatcagcgg caatatcgca acaaattatt gataacgcca
     4981 ggtttgacta ccagcaggtc gcagcaaagg agcgcaaagc cgcgaacgat ttcctggtgg
     5041 agaagcaacg gctggcagtt ttatctgctc aggaagaaaa cgtacgtgcg agtatcgaag
     5101 aagttcaggc cgctttgact caggcactac tggatctgga atataccct
//
LOCUS       DAEEGA010000186         5026 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEEGA010000186 DAEEGA010000000
VERSION     DAEEGA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.187"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            50..124
                     /gene="tnaC"
                     /locus_tag="KLH46_004030"
     CDS             50..124
                     /gene="tnaC"
                     /locus_tag="KLH46_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HBE0380496.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            345..1760
                     /gene="tnaA"
                     /locus_tag="KLH46_004031"
     CDS             345..1760
                     /gene="tnaA"
                     /locus_tag="KLH46_004031"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBE0380497.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYRKEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1851..3098
                     /gene="tnaB"
                     /locus_tag="KLH46_004032"
     CDS             1851..3098
                     /gene="tnaB"
                     /locus_tag="KLH46_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBE0380498.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3230..4405
                     /gene="mdtL"
                     /locus_tag="KLH46_004033"
     CDS             3230..4405
                     /gene="mdtL"
                     /locus_tag="KLH46_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBE0380499.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4380..>5026
                     /gene="yidZ"
                     /locus_tag="KLH46_004034"
     CDS             4380..>5026
                     /gene="yidZ"
                     /locus_tag="KLH46_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBE0380500.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1146 a         1209 c         1255 g         1416 t
ORIGIN      
        1 attctgagtg taataatgta gcggcgtgtc tttcgaggat aagtgcatta tgaatatctt
       61 acatgtatgt gtgacctcaa aatggttcaa tatcgacaat aagattgtcg atcaccgccc
      121 ttaatttacc cttctgtagc catcaccaga gccaaaccga ttagattcaa tgtgatcgat
      181 tagtttgcta tatcttaatt ttgccttttg caaaggccat ctctcgttta tttacttatt
      241 ttagtaaatg atggtgcttg tatatataca tggtgaatta atggatattg cagatgtaat
      301 attcacaggg atcactgtaa ttaaaataaa tgaaggatta tgtaatggaa aactttaaac
      361 atctccctga accgttccgc attcgtgtta ttgagccagt aaaacgtact actcgcgctt
      421 atcgtaaaga agcaattatt aaatccggta tgaacccgtt cctgctggat agcgaagatg
      481 tgtttatcga tttactgacc gacagcggca ccggggcggt aacccaaagt atgcaggcag
      541 cgatgatgcg cggcgacgaa gcctacagcg gcagccgcag ctactatgcg ttagccgagt
      601 cagtgaaaaa tatctttggt tatcaatata ctattccgac tcaccagggc cgtggcgcag
      661 agcaaatcta tattccggta ctgattaaaa aacgcgagca ggaaaaaggc ctggatcgca
      721 gcaaaatggt ggcattctct aactatttct ttgataccac gcagggccat agccagatta
      781 acggctgtac cgtgcgtaac gtctatatca aagaagcctt cgatacgggc gtgcgttacg
      841 actttaaagg caactttgac ctcgaaggat tagaacgcgg tattgaagaa gttggcccga
      901 ataacgtgcc gtatatcgtt gcaaccatca ccagtaactc tgcaggtggt cagccggttt
      961 cactggcaaa cttaaaagcg atgtacagca tcgcgaagaa atacgatatt ccggtggtaa
     1021 tggactccgc acgctttgct gaaaacgcct atttcatcaa gcagcgtgaa gcagaataca
     1081 aagactggac catcgagcag atcacccgcg aaacctacaa atatgccgat atgctggcga
     1141 tgtccgccaa gaaagatgcg atggtgccga tgggcggctt gctgtgcatg aaagacgaca
     1201 gcttctttga tgtgtacacc gagtgcagaa ccctttgcgt ggtgcaggaa ggcttcccga
     1261 catatggcgg cctggaaggc ggcgcgatgg agcgtctggc ggtaggtctg tatgacggca
     1321 tgaatctcga ctggctggct tatcgtatcg cgcaggtaca gtatctggtc gatggtctgg
     1381 aagagattgg cgttgtctgc cagcaggcgg gcggtcacgc ggcattcgtt gatgccggta
     1441 aactgctgcc gcatatcccg gcagaccagt tcccggcaca ggcgctggcg tgcgagctgt
     1501 ataaagtcgc cggtatccgt gcggtagaaa ttggctcttt cctgttaggc cgcgatccga
     1561 aaaccggtaa acaactgcca tgcccggctg aactgctgcg tttaaccatt ccgcgcgcaa
     1621 catatactca aacacatatg gacttcatta ttgaagcctt taaacatgta aaagagaacg
     1681 cggcgaatat taaaggatta acctttacct acgaaccaaa agtattgcgt cacttcaccg
     1741 caaaactgaa agaagtttaa ttaatactac agagtggcta taaggatgtt agccactctc
     1801 ttaccctaca tcctcaataa caaaaatagc cttcctctaa aggtggcatc atgactgatc
     1861 aagctgaaaa aaagcactct gcattttggg gtgttatggt tatagcaggt acagtaattg
     1921 gtggaggtat gtttgcttta cctgttgatc ttgccggtgc ctggtttttc tggggtgcct
     1981 ttatccttat cattgcctgg ttttcaatgc ttcattccgg gttattgtta ttagaagcaa
     2041 atttaaatta tcctgtcggc tccagtttta acaccatcac caaagattta ataggtaaca
     2101 cctggaacat tatcagcggt attaccgttg ccttcgttct ctatatcctc acttatgcct
     2161 atatctctgc taatggtgcg atcattagtg aaacgatatc aatgaatttg ggctatcacg
     2221 ctaatccacg tattgtcggg atctgcacag ccattttcgt tgccagcgta ttgtggataa
     2281 gttcgttagc cgccagtcgc attacctcat tgttcctcgg gctgaagatt atctcctttg
     2341 tgatcgtgtt tggttctttc ttcttccagg tcgattactc catcctgcgc gatgccacca
     2401 gcaccactgc gggaacgtct tacttcccgt atatctttat ggctttgccg gtgtgtctgg
     2461 cgtcatttgg tttccacggc aatattccca gcctgattat ttgctatggc aaacgcaaag
     2521 ataagttaat caaaagcgtg gtatttggtt cgctgctggc gctggtgatt tatctcttct
     2581 ggctctattg cacgatgggg aatattccgc gcgaaagctt taaggcgata atctcctcag
     2641 gcggcaacat tgattcgctg gtgaaatcgt tcctcggcac caaacagcac ggcattatcg
     2701 agttttgcct gctggtgttc tctaacttag ctgttgccag ttcgttcttt ggtgtcacgc
     2761 tggggttgtt cgattatctg gcggacctgt ttaagattga taactcccac ggcgggcgtt
     2821 tcaaaaccgt gctgttaacc ttcctgccac ctgcgttgtt gtatctgatc ttcccgaacg
     2881 gctttattta cgggatcggc ggtgccgggc tgtgcgccac catctgggcg gtcattattc
     2941 ccgcagtgct tgcaatcaaa gctcgcaaga agtttcccaa tcagatgttc acggtctggg
     3001 gcggcaatct tattccggcg attgtcattc tctttggtat aaccgtgatt ttgtgctggt
     3061 tcggcaacgt ctttaacgtg ttacctaaat ttggctaaat ccttcaagaa gccagccatt
     3121 cgctggcttc ttgcctctca ggaaatcact tatgtccaaa tggcaactcg ccttatcctc
     3181 cttcaccacg tatgctttgc gtcaccttac tatcaggacg ctttagccca tgtcccgctt
     3241 tttgatttgt agttttgccc tggttttact ttatcccgcc gggattgata tgtacctcgt
     3301 tggtttaccg cgcatcgccg ccgatctcaa tgccagcgaa gcgcagttgc atattgcgtt
     3361 ctccgtatat ctggcgggga tggcagctgc gatgctattt gccggtaaag tggccgatcg
     3421 ttcagggaga aagccggtcg ccatacccgg cgcggcgcta tttattattg cctcggtgtt
     3481 ctgttcactg gctgaaacca gcacgttatt tcttgcaggc cgatttctac aggggttggg
     3541 cgcaggctgt tgttacgtgg tggcgttcgc cattttgcgc gacacgctgg atgatcgacg
     3601 tcgggctaaa gtgctgtcat tactcaacgg tattacctgc atcattccgg tgttagcgcc
     3661 agtgctcgga catctgatta tgcttaaatt cccgtggcag agtctgttct gggcgatggc
     3721 aatgatgggc atcgcggtac tgatgttgtc tttgtttatt ttaaaagaaa cgcgcccagc
     3781 gtcccccgct gcttcggaca aaccacgaga aaatagcgag tcgctgctta atcgtttttt
     3841 cctcagccgt gttgttatca ccaccctcag cgtttcggtg atcctcactt tcgtcaatac
     3901 atcgccggta ttgctgatgg aaatcatggg gtttgagcgc ggagaatacg ccaccattat
     3961 ggcgctgacc gctggcgtca gcatgacctt ttcattctcg acgccatttg cgctgggaat
     4021 ttttaagcca cgtacgttga tgatcacctc gcaggtgtta ttccttgcgg cggggatcac
     4081 ccttgccgtt tcaccttccc atgcggtttc tctgtttggt atcacgctga tttgcgccgg
     4141 tttctcggta ggttttggcg tagcgatgag tcaggcgtta ggaccatttt cattacgcgc
     4201 gggcgtagcc agctcgacct taggtattgc gcaggtttgc ggttcgtcac tgtggatttg
     4261 gctggccgcg gtggttggta tcggcgcatg gaatatgctg atcgggattc tgattgcctg
     4321 tagcatagtg agcctgttgc tgattatgtt cgtcgcgcct ggacgccccg ttgccgctca
     4381 tgaagaaatc catcaccacg cttgatctca atctgctgct ctgtctgcaa ctgctgatgc
     4441 aggagcgcag cgtgaccaaa gcggcgaagc ggatgaacgt gaccccttca gcggtgagta
     4501 agtcgctggc aaagttaaga gcgtggtttg acgacccgct ctttgtgaac tcaccgctgg
     4561 ggctgtcgcc cacacagcta atggtcagca tggagcaaaa tctggcggaa tggatgcaaa
     4621 tgagcaatct gctgctggat aaaccgcacc accagacacc gcgcggcctg aagtttgagt
     4681 tggcggcgga atcgccgctg atgatgatca tgcttaatgc gctgtcgaaa cggatctacc
     4741 aacgttaccc gcaggcgacc atcaaactac gtaactggga ttacgattcc ttagatgcca
     4801 ttactcgtgg tgaagtggat attggttttt ccggtcgcga aagccatcct cgctcgcggg
     4861 agctgttaag ctcgctaccg ttagccattg attatgaagt gctgtttagt gatgtaccct
     4921 gcgtctggtt acgcaaagat catccggcac tgcatgaaac gtggaatctg gacacctttt
     4981 tacgttatcc gcatatcagc atttgctggg aacagagcga cacctg
//
LOCUS       DAEEGA010000187         5018 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEEGA010000187 DAEEGA010000000
VERSION     DAEEGA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.188"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..249
                     /locus_tag="KLH46_004035"
     CDS             <1..249
                     /locus_tag="KLH46_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBE0380501.1"
                     /translation="SLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSMPELEELAAA
                     MVQAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(450..1307)
                     /gene="sitD"
                     /locus_tag="KLH46_004036"
     CDS             complement(450..1307)
                     /gene="sitD"
                     /locus_tag="KLH46_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBE0380502.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1304..2161)
                     /gene="sitC"
                     /locus_tag="KLH46_004037"
     CDS             complement(1304..2161)
                     /gene="sitC"
                     /locus_tag="KLH46_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBE0380503.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2158..2985)
                     /gene="sitB"
                     /locus_tag="KLH46_004038"
     CDS             complement(2158..2985)
                     /gene="sitB"
                     /locus_tag="KLH46_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBE0380504.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2985..3899)
                     /gene="sitA"
                     /locus_tag="KLH46_004039"
     CDS             complement(2985..3899)
                     /gene="sitA"
                     /locus_tag="KLH46_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBE0380505.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4350..>5018)
                     /locus_tag="KLH46_004040"
     CDS             complement(4350..>5018)
                     /locus_tag="KLH46_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE0380506.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLSSLQSLPP
                     ICPI"
BASE COUNT         1339 a         1223 c         1204 g         1252 t
ORIGIN      
        1 tcactgggcc gcctgtcccc ggtagtcatg ctggcaaaat ctgtcacagc acaaacacat
       61 atggcgtgga gcgatgccga taatcagcag gtgaaactgt cgatgccgga actggaagaa
      121 ctggcggcag caatggtgca ggcgcaggtc gatcgcaacg atgagattta tcgccgtcag
      181 cgggagctga aggaagagct gaatagtctg aaggatttga attcggttag gaattttatc
      241 gtggaataac agaagctgcg gcacgtcgta tgcaggaacg tgccgcggtt ggctggtaaa
      301 ctttcgatag tgcgagtatt gaatgatttc taggcgttat cgattttacg tattttttgc
      361 atgatagaat tcgtacctcc tcccaacgac cttccatggc tttccggact tctgtaggct
      421 ggcggacgat gctgtcctcg gatgttgtgc taattgggtg ataacagatc ctgtgtatgg
      481 gcgtttccct ttgtgcgtgc gttgatgctg gtaacggtaa acgtcatgat gaacacgacc
      541 gcgaataaga cgacaatcgt cggagctggt gcgctgtcga taaaaaagga cagccagaca
      601 ccagtcattg acactattac cgatatgcca gtcgccagta ggagcgcaat atgaaagcgt
      661 tgtgtcagga gtactgcaat ggcgccaggc gcaatgagca gagaaataga gagaatgata
      721 cctactgctt tcagcgtcgc cacaatggtc agggaaacca tgcacagcag cccataatgc
      781 agccatctcg tatgcaaacc gcttacctgc gcctgttgat aatcaaagct gaacagcaaa
      841 aaatcccgcc attttacgct aataacaagt gtaaccagcc cggcaataat caccgtctgg
      901 attatatcgc cgatggttat cccgagcatg tcgccgaaaa gaatatggtc aagatgtacg
      961 tctggtttaa ttgcaatata caagatcaga cctgcggcaa acatgccaga aaaaacaatc
     1021 cccataactg tatcttgctt gattcggctg ttgtctttca ggtatcccgt cgccaccgcg
     1081 cagaatactc cggcgacgaa tgcccctgta gccagaggta accccagaat ccaggtaagc
     1141 acgatgccag gaaaaaccgc atgactcatc gcgtcaccca tcagtgccca acctttcagg
     1201 accagaaaga ccgacagcag cgcacagggt atggaaacta ccagtgaaat cagcagtgcg
     1261 tgactcataa aggtgaactg cagtggctcc agtaataatg ccatcatgag cgatcctcca
     1321 gcgctttatg cgcacgccga cgatttgcca aaagtccatg ggtgggcgca aatacaaatg
     1381 ctaaaagaaa cagcagtgtc tgcgccacca cgatgattcc acctgtcgcg ccgtccagaa
     1441 agtaactgac ccacgcgccg aggaaactgg ttacactgcc aatagttaca gcaattatga
     1501 gcaggcgggg aaaacgatcg gttaacaacc atgctgttgc accgggagtc accaccagac
     1561 agatcaccag aaacgcacca accgtttgca gcgccgcaac ggtcgaaacc gacagcaggg
     1621 tgaagaagag gatttttaat ctgcccggat gtaatccaat agcacgggcg tgattctcgt
     1681 cgaaaaaggt caccataaga tctttccatt tgaaaaaaag cacgatgatc gagagaatgc
     1741 caatgatagt gagttgcagg atatccgccg gatcgattgc cagaatattg cccaggacga
     1801 tggtctgaat gttcacggat gtcgggttca atgacaccat aaacagcccc aggccaaaaa
     1861 aggacgagaa gatcaggccg atgatggcat cttcttttaa gcgggtgcgt tggttaagaa
     1921 acagcatgct gcccgctgcc agtccgccgg aaaagaaggc tcccagagaa aacggaagcc
     1981 ccagcatata tgcccccgca acacccggga caatagagtg cgacagcgca tcgccaatca
     2041 gcgaccagcc ttttagcatc agatagcatg acaggaaagc gcagagaccg ccgaccattg
     2101 ccgagaccca catcgcattg agcatatatt catagctgaa gggttccagt agcacgttca
     2161 tcttgcttcc ctctgcaccg ctgacggtcg atgtgccaca aaagggcgtt catcgtcagt
     2221 aataatgctt tcttccgagc cgttaagtgt gacatggcgc agtacgccgc taaaagccag
     2281 ctccaggttc tcggctgtaa aagtggtgtc tgtcggcccg cttgccagga cggtgccttt
     2341 gaccatgacc gtataatcgc aaaatgtcgt gactgagccg agattgtgcg ttgagacaag
     2401 catcgttttg ccttccgcgc gtaactccct caacaggctg atgattttcg cttcggtttt
     2461 cacatcaacg ccggtaaaag gttcatccag caggatcaca tcaccctgct gtgcaattgc
     2521 tctcgccaga aagacgcgtt tcttttgtcc accggaaagc tcgccgattt gacgatggcg
     2581 aaaatccacc atatcgacac gttccagcgc atcggtgaca atctgacgat ccctcttttt
     2641 ggctatacgc aacataccca tatgcccata gcggcccatc atcaccacat cttccaccag
     2701 tacaggaaat gaccagtcaa cctcttctga ctgagggacg taggcaacca ggtttttctg
     2761 tagcgcctgt cgcgtgggaa tacccagaac agatatcttt ccgctggtca gacgcacaaa
     2821 tcccataatc gctttaaaca acgtcgattt tccggaaccg tttaccccaa ccagagcggc
     2881 aatagatcca cttggtactg tgaaggatgc atcgcgcagt gctgtgtgcc cgttacgcca
     2941 ggtgacagta acatcattaa cgacaatgcc tgcagattgc atcattattt ctcccttttc
     3001 ccggctttaa ttccctgtac cagcgtactg gtagtaacct taagaaggtc gatatacgta
     3061 ggtaccgggc cgttttctgt gctcagggaa tcgacataga gcacaccgcc gtagtgtgcg
     3121 ccggtttcac gcgcaacctg acgcgctggt ttatcggaaa tcgtactctc gctaaagact
     3181 gccgggatat gatttttctt aactatatca acaaccttac gtacctgctg cggtgttcct
     3241 tgttgatcgg cattaatcgg ccacagataa agctctttta gccccaaatc gcgtgcgaga
     3301 taagaaaaag ccccttcact ggtgaccatc catcgctgat tctcagggag ttccgtaatc
     3361 tgcttacgca ggggggcaag ggtttgggta atcttggctt tataagtatc ggcattacgt
     3421 tggtaggttt gtgcatttgc cggatcgtat tttatcaacg catcacgaat attatcgacg
     3481 taaatcagag cattatctgg cgacatccat gcatggggat taggtttgcc ctcatagggt
     3541 ccttcggtga tccctactgg cgtcacaccc gaagagacaa ttacttctgg aaccccattg
     3601 agatgctggt aaaagcgttg gaaccacaat tccagattca taccattggc gagaatcagt
     3661 tgtgccccct gcgcacgttt aatatcgcca ggggtaggct gatactcatg aatttctgca
     3721 ccaggcttgg ttatggatga gacttctgca gcatctccag ccacgttttt ggccatatcc
     3781 gcgatgatgg taaatgttgt aatgaccttg aatttttcag ttgcacttgc ctgaaatgcg
     3841 atcgagcagg tgagggcgag gcaccccaag agcatggtta ctttttttat tgagtgcata
     3901 atttagtacc tatagtgagc aaaattagaa caaacatagc aacggctatg ttttatagca
     3961 taacctatac aaaatgaaaa taattatcat taacagcttt gaggcctaaa acagggatta
     4021 ggttgtttcc tgctggcgac atgatattga ggaaaaaagt agagcagcta ttaggctttg
     4081 aaatgacttt atgaggaaaa ttgttgatgg caaaaatggg gcaaaacgct gtataagggg
     4141 caaaaaaggg gcaaaaaagg ggcaaaaaag gggcaaaaaa gagtgggtta tcgtagctta
     4201 ttgttgtcgc tgataatgtt taacatattg aaaaataaat aaaatactta tgagtcagat
     4261 agttgtgatt tttgccctta cttgttcagg tcgtattgtt ctttctttgt aatttgttga
     4321 ttttcttgca ttatttcagt tctctggtac taaatggggc aaattggggg caaactttgc
     4381 aacgacgata accgcgcatt caacatggct atctgttcgt cgttcatgtc atcaatccac
     4441 ataccgtaaa tttcatacac catttgcgca gtttcatgcc ccatctggct ggctataaat
     4501 gccgggttcg ctcctgccgt caacagccag caggcaaaag tatgtcgcgt gtggtacgga
     4561 ttacggcggc gaataccagc acgttttact gctgcattcc atcttgtacc caaactgctt
     4621 accgagtaat aaggtttctg tttttcgtta cacaccctgg gcatgaaaac aaaatgcagt
     4681 ttttgttttt cagttctgcc gtactcccga tgataaaaag tgatttcgct tttgcgatga
     4741 tgcccggtca gtttgtattg ctccttcagt gcttcaagag ccggctgcag taatgttacc
     4801 gtccggatcc cggcatttgt ttttggggga ccgaacatat caagtatcgt caggtttctt
     4861 ctgacattca caattccctt ctcgaaatcc acatcctccc acgccagagc tgccagttcc
     4921 ccgtgacgaa gcccggagta aacggcaaat ttccacaagt tttggctctg tcctttttca
     4981 ctttccatta atgcattgaa ttctgtttta gataacgg
//
LOCUS       DAEEGA010000188         4972 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEEGA010000188 DAEEGA010000000
VERSION     DAEEGA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.189"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(183..767)
                     /gene="kptA"
                     /locus_tag="KLH46_004041"
     CDS             complement(183..767)
                     /gene="kptA"
                     /locus_tag="KLH46_004041"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBE0380507.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1017..1700
                     /locus_tag="KLH46_004042"
     CDS             1017..1700
                     /locus_tag="KLH46_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HBE0380508.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1697..2158
                     /locus_tag="KLH46_004043"
     CDS             1697..2158
                     /locus_tag="KLH46_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBE0380509.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLSGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2171..3343
                     /gene="iadA"
                     /locus_tag="KLH46_004044"
     CDS             2171..3343
                     /gene="iadA"
                     /locus_tag="KLH46_004044"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBE0380510.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3408..4319
                     /gene="hypT"
                     /locus_tag="KLH46_004045"
                     /gene_synonym="qseD"
     CDS             3408..4319
                     /gene="hypT"
                     /locus_tag="KLH46_004045"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBE0380511.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4312..4704)
                     /gene="iraD"
                     /locus_tag="KLH46_004046"
     CDS             complement(4312..4704)
                     /gene="iraD"
                     /locus_tag="KLH46_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBE0380512.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4701..4784)
                     /gene="idlP"
                     /locus_tag="KLH46_004047"
     CDS             complement(4701..4784)
                     /gene="idlP"
                     /locus_tag="KLH46_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HBE0380513.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1174 a         1303 c         1240 g         1255 t
ORIGIN      
        1 tgaccggaag cgacgctgat tccagtcccc aactgaccgc ctgccgtaaa tcactttaat
       61 ttcagcagtt gttattaaag gatacccttt tacgagggta tcccttcaga ctgataacaa
      121 acgctacatt gccggatgtg ctgcgcttac caggcctata taacccctgc aatatattga
      181 atttattgtt tttgtacccc agtcggcata aacgaccact caagaaactc aacagcaacc
      241 gttgatgtca gccagacccc gttttccgct tgccagaaag ggatgcctcg tttcgccatt
      301 tcctgtgctt tgacggttaa gacaaccggc gatccgtgtc ttgctcccac tttgcgtgcc
      361 gttgcttcat cagcagagag atgcacgtaa tgacgatccc ctgcaatcag cccctgtttt
      421 tttatctcgt caaggaaacg gcttgccgta ccgtggtaga gaaactgtgg cggtgttttt
      481 tcggcaaagg aaatagcgac ctgcgaagtc gaatgcccct gaaccgcgcg gatgcaccta
      541 ccatcactgg aataactaaa acgcttttta tcgctggttg cgaccacggt atccagcaat
      601 gcacgggtta atcgcttgcc cgctttttgg gcacaaagta tgagcttatt gatatccgcc
      661 catccttcac ggtccagtac gataccaatc gcctccggtt tgtgtcggag gacaaaactt
      721 aaaaacttgc tcgtatccgc cagctctttt tcgttgtact ttgccatcgc gttttcgtcc
      781 ctggattttc tgtttttatt tactaatgtc attgtcactg ggtttatgaa ttttctgcaa
      841 ctcaatcctg cgcagaacct tttccacaca gcgtaatttt tcccctatgc aaaaaacgca
      901 tagccagaaa catacttgca ttttccccat acccacatgg ctgataaggt ttttcagttc
      961 caggaatttt cctggctcaa gcaacacaca aacacaaaaa aacaatgggg attgttatga
     1021 cacaacaagg ggatgctgta gccggggagc tggcgacaga aaaagtgggc attaaagggt
     1081 atctggcttt ttttctcacc ataatctttt ttcccggtgt tttttccgga acagacagct
     1141 ggtggcgcgt ttttgatttt tccgttctga acggatcgtt tgggcaactg cctggtgcga
     1201 atggagccac cacctcattt cgcggggtgg gtggcgcggg ggctaaagat ggctttctct
     1261 ttgcgctgga gctggcaccg tcagtcattc tctctctggg gattatttca attaccgatg
     1321 gtcttggtgg attacgcgcc gcgcagcagt taatgacacc tgtgctaaaa ccgttgttgg
     1381 gcattccagg gatctgctcg ctggcactga tcgctaacct gcaaaacacg gatgccgccg
     1441 ccggtatgac caaagagctg gcgcaggaag gtgaaattac cgaacgcgat aaagtgattt
     1501 tcgccgcata tcaaaccagc ggtagcgcca tcatcaccaa ctacttctct tccggcgtcg
     1561 cggtgtttgc ctttctgggc acgtccgtaa tcgtaccttt agccgtcatc ctggtgttta
     1621 aatttgtcgg agccaacatt ttgcgcgtct ggcttaactt cgaagaacgt cgcaacccaa
     1681 cgcaaggagc gcaagcatga caactcaggt ccgcaaaaac gtcatggata tgtttatcga
     1741 tggcgcgcgc cgtggcttta ctatcgccac gaccaacctg ctgccaaacg tggtgatggc
     1801 gtttgtcatc attcaggcgc tgaaaattac cggcctgctc gactgggtgg ggcatatctg
     1861 tgaacccgta atggcgctgt ggggattatc cggtgaagcg gcaaccgtcc ttttagccgc
     1921 attaatgagt atgggcggcg cagtcggtgt tgccgccagt ctggcaaccg ctgacgcatt
     1981 aaccggccac gatgtcaccg ttttactgcc cgcaatgtat ttaatgggca acccggtgca
     2041 aaatgtcggt cgctgcctgg ggacagccga agtgaatgcc aaatattacc cgcatatcat
     2101 tacggtctgc gtgattaacg cattgctgtc gatctgggtt atgcagctta ttgtttaaca
     2161 aggagttacc atgattgatt ataccgcagc cggttttacc ctgctgcagg gagcgcattt
     2221 gtatgcgccg gaagatcggg gaatttgcga tgtcctcgtc gctaacggca aaattatcgc
     2281 cgttgccagc aatatccctt ctgacattgt accgaactgc acggttgtcg atctcagcgg
     2341 gcagatcctc tgcccaggtt ttattgatca acacgtccat ttgattggcg gtggcggcga
     2401 agcaggtccc acgacgcgca cgccggaagt ggcgctaagt cgcctgacgg aagcgggcgt
     2461 cacgtcagtg gttggtctgc tgggcaccga ctctatctct cgccacccgg aatccctgct
     2521 cgccaagacc cgtgcgctca atgaagaagg catcagcgcc tggatgctga ccggcgctta
     2581 tcatgtccct tcccgcacta ttacgggttc cgtggaaaaa gacgtggcga ttatcgatcg
     2641 tgtgattggc gtgaaatgcg ccatctctga tcaccgttct gccgcaccgg acgtttatca
     2701 cctggctaat atggcggcag aatcccgcgt tggcggtttg ctcggcggta aacctggcgt
     2761 caccgtgttc cacatgggcg acagtaaaaa ggcgttaaag cctatttatg acctgctgga
     2821 aaactgcgat gtgccgatca gcaagctgct gccgacccac gttaaccgca acgtaccgtt
     2881 gtttgagcag gcgctggagt tcgcgcgcaa aggcggcacc atcgatatca ccagcagcat
     2941 tgacgaaccg gtcgcccctg ccgaaggtat tgcccgcgcc gttcaggcgg gtattccgct
     3001 ggcacgcgtc accctcagct ccgacggcaa cggtagccag ccgttcttcg atgacgaagg
     3061 gaatttaacc catatcggtg ttgcaggttt tgaaacgttg ctggaaaccg tgcaggtgct
     3121 ggtcaaagac tatgatttca gtatcagcga tgccctgcgc ccgctcacca gtagcgtagc
     3181 cggtttcctt aacatgagcg ggaaaggcga aattctgcca ggcaatgacg cagacttact
     3241 ggtcatgacg ccagaattgc gcattgagca ggtatacgct cgcggcaaac tgatggtcaa
     3301 agacggcaaa gcctgcgtga aaggaacgtt tgaaacggct taaacggttg tcagccgccc
     3361 taatggacgt agaatgcccc caagggcggc tgacagagta aaacgtaatg gatgactgtg
     3421 gtgcggtttt gcataatatt gaaaccaaat ggctttatga ttttctgact ctggaaaaat
     3481 gccgcaattt ttcccaggcg gcagtcagtc gcaacgtctc gcaaccggca ttcagccgcc
     3541 gcatccgtgc actggaacag gcgattggcg ttgaattgtt taaccgccag gtgacgccac
     3601 tgcaactctc agaacaaggg aaaatcttcc attcgcagat ccgccatctg ttgcaacagt
     3661 tagaaagcaa ccttgcagag ctgcgtggcg gcggcgatta cgcgcaacgt aaaatcaaga
     3721 tagccgctgc acactctctt tccctcggtc tgttaccgtc cattatcagc cagatgccgc
     3781 cgctctttac ctgggcaatt gaagccattg atgtcgatga agcggtcgat aaactgcgtg
     3841 aagggcaaag tgactgtatt ttttcctttc acgacgaaga tttgctggaa gcgccgtttg
     3901 accacattcg cttatttgaa tctcaattgt tccctgtctg cgccagtgac gaacacggag
     3961 aagcactttt taacctcgcg cagccacact ttccgttact gaattacagc cgcaactcct
     4021 acatggtgcg attgattaat cgcaccctga cgcgccacag tgagttaagt ttcagcacct
     4081 tttttgtctc ttcgatgagc gagcttttaa agcaggttgc cctcgacggc tgtgggattg
     4141 cctggctgcc ggagtatgcc atccaacaag aaattcgcag cgggcagctc gttgtgctga
     4201 atcgggacga acaggtgatc ccgattcagg cctacgcata ccggatgaat acccgcatga
     4261 atccagttgc cgaacgcttc tggcgtgaac tgcgcgagct ggagattgtg cttagctgac
     4321 attctcaagc gtcgcactgc gccaaccgcc attatcccag accagatcaa acgttagcgg
     4381 ttcatcgtcg tggtacagca ttatctcaag gctgatcgcc agagtgtggc agtacgcttc
     4441 tttcaaatta acctgcaacg ccgcaatccg tggctcatga aataacagtg cagaacgcag
     4501 ctgacggcaa taccagctct ggagatgttt gtcgctcggg ttatgcactt gccactcggg
     4561 catgcctgaa acaagtaatg acggtaatgc cgaatgagga gaattaaaaa gagtgaggag
     4621 atcgctgcat accgatttac gcagcaatga cgttttattg cctgaaattt caggtaaaac
     4681 agcctgaagt gattggcgca tcattttgcg cactcctgac gtttagcaac atatgcggct
     4741 tgccctgaac accgggcacc actgtattga tgctcatttt ccatagccat tcccttttgt
     4801 tcattacatc catgtcagtc tgagacaggc ctgaatcctg tcagcaaagt ataaaacctt
     4861 acttgcctat gaatatctac tgacttttca ggaaaaaagt catgatttga taaaaatcat
     4921 caaactcaaa aaaaaaacgc tagtatattc tgaatattcc gaatagttac gt
//
LOCUS       DAEEGA010000189         4874 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEEGA010000189 DAEEGA010000000
VERSION     DAEEGA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.190"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..530
                     /locus_tag="KLH46_004048"
     CDS             <1..530
                     /locus_tag="KLH46_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBE0380514.1"
                     /translation="RTTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSIT
                     LLNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSV
                     IFINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLK
                     HDAVLIEAMESRSLR"
     gene            727..2166
                     /gene="aldA"
                     /locus_tag="KLH46_004049"
     CDS             727..2166
                     /gene="aldA"
                     /locus_tag="KLH46_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBE0380515.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2208..3209)
                     /gene="gap"
                     /locus_tag="KLH46_004050"
     CDS             complement(2208..3209)
                     /gene="gap"
                     /locus_tag="KLH46_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBE0380516.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3398..3928
                     /gene="cybB"
                     /locus_tag="KLH46_004051"
     CDS             3398..3928
                     /gene="cybB"
                     /locus_tag="KLH46_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBE0380517.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4173..4346
                     /locus_tag="KLH46_004052"
     CDS             4173..4346
                     /locus_tag="KLH46_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380518.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4418..4567)
                     /gene="hokB"
                     /locus_tag="KLH46_004053"
     CDS             complement(4418..4567)
                     /gene="hokB"
                     /locus_tag="KLH46_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBE0380519.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4668..4808)
                     /locus_tag="KLH46_004054"
                     /pseudo
     CDS             complement(4668..4808)
                     /locus_tag="KLH46_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1122 c         1208 g         1297 t
ORIGIN      
        1 tccgcaccac tggcagagca gaagcgacca tcctggtgga tatcgctcat cagttctggc
       61 atattccgca tgaaaaaatc tggattgaag accagtcaac aaactgcggt gaaaacgcac
      121 gctttagcat cacgctattg aatcaggccg tagaacgagt tcatacggct atcgttgtac
      181 aggaccccac catgcagcgg cgcacgatgg caacgttccg ccgtatgacc ggggacaatc
      241 ccgatgcacc acgctggtta agttatcccg gattcgttcc tcagttagga aataacgcag
      301 acagtgtaat ctttattaat cagttacaag gattatggcc ggttgagcgt tatctctcac
      361 tactcactgg cgagctgccg cgtttacacg atgatagcga tggctacggt ccccgcgggc
      421 gagattttat cgttcacgtt gattttccgg cagaagtcat ccatgcatgg caaacgctga
      481 aacatgatgc ggtgctcatc gaggcgatgg aaagtcgctc gttacgttaa aaattgcccg
      541 tttgtgaacc acttgtttgc aaacgggcat gactcctgac ttttatttct gccttttatt
      601 ccttttacac ttgtttttat gaagccctcc acagacttgt cctttcccga tgccgtctct
      661 ctgatgattg atgttaatta acaatatatt caccgaaaac aaacatataa atcacaagag
      721 tcgcccatgt cagtacccgt tcaacatcct atgtatatcg atggacagtt tgttacctgg
      781 cgtggagacg catggattga tgtggtaaac cctgctacag aggctgtcat ttcccgcata
      841 cccgatggtc aggccgagga tgcccgtaag gcaatcgatg cagcagaacg tgcacaacca
      901 gaatgggaag cgttgcctgc tattgaacgc gccagttggt tgtgcaaaat ctccgccggg
      961 atccgcgaac gcgccagtga aatcagtgcg ctgattgttg aagaaggggg caagatccag
     1021 cagctagctg aagtcgaagt ggcttttact gccgactata tcgattacat ggcggagtgg
     1081 gcacggcgtt acgagggcga gattattcaa agcgatcgtc cgggagaaaa tattcttctg
     1141 tttaaacgtg cgcttggtgt gactaccggc attctgccgt ggaactttcc gttcttcctc
     1201 attgcccgca aaatggctcc cgctcttttg accggtaata ccatcgtcat taaacccagt
     1261 gaatttacgc caaacaatgc gattgcattc gccaaaatcg tcgatgaaat aggccttccg
     1321 cgcggcgtgt ttaaccttgt actggggcgt ggtgaaaccg ttgggcaaga actggcgggt
     1381 aacccaaagg tcgcaatggt cagtatgaca ggcagcgtct ctgcaggtga gaagatcatg
     1441 gcgactgcgg cgaaaaacat caccaaagtg tgtctggaat tgggtggtaa agcaccggct
     1501 atcgtaatgg acgatgccga tcttgaactg gcagtcaaag ccatcgttga ttcacgcgtc
     1561 attaatagtg ggcaagtgtg taactgtgca gaacgtgttt atgtacagaa aggcatttat
     1621 gatcagttcg tcaatctgct gggtgaagcg ttgcaggcgg ttcaatttgg taaccccgct
     1681 gaacgcaacg acattgcgat ggggccgttg attaacgccg cggcgctgga aagggttgag
     1741 caaaaagtgg cgcgcgcagt agaagaaggg gcgagagtgg cgttgggtgg caaagcggta
     1801 gaggggaaag gatattatta tccgccgaca ttgctgctgg atgttcgcca ggaaatgtcg
     1861 attatgcatg aggaaacctt tggcccggtg ctgccagttg tcgcatttga cacgctggaa
     1921 gatgctatct caatggctaa tgacagtgat tacggcctga cctcatcaat ctatacccaa
     1981 aatctgaacg tcgcgatgaa agccattaaa gggctgaagt ttggtgaaac ttacatcaac
     2041 cgtgaaaact tcgaagctat gcaaggtttc cacgccggat ggcgtaaatc cggtattggc
     2101 ggcgcagatg gtaaacatgg cctgcatgaa tatctgcaga cccaggtggt ttatttacag
     2161 tcttaatgag tgaaagaggc ggaggttttt tcctccgcct gtgcgcgtca gagtttagcg
     2221 aatttttcga gggtgcgaat aagctgcgtg acgaagccat attcgttatc gtaccaggcg
     2281 accgttttca ccagttgtaa atcgcccacg gcggtaattt ccgtttgcgt ggcatcaaac
     2341 actgaaccga aatggctgcc aatgatatcg gaagagacta tttcttcatc ggtataacca
     2401 aatgactcgt tattggtggt tgcttgttta agtgcgttat tcacctcttc ggcagtcact
     2461 ttttttccga gaatggacac cagctcagtg accgaacctg ttttcaccgg cacgcgttgc
     2521 gcatgacctt tcagtttgcc gctcagttcc gggatcacca gaccaatggc ttttgccgcc
     2581 cccgtagtgt ggggaatgat attttctgcc gctgcgcgtg aagcacgtaa atctttacca
     2641 cgcgggccat ccaccagtga ctgggtgcca gtataggcat gaatggtcgt catcgtgccg
     2701 acttctatcc cgaaactgtc atgcaaggct ttggccatcg gcgcaaggca gttagtggtg
     2761 catgacgcca cggaaacaat ggtgtcgttg ccatccagag tgtcgtcatt aacgttataa
     2821 acgatggttt tcatttcacc ggcaggagcg gaaatcaaca ccttcttcgc accagcatca
     2881 agatgcgcct gcgatttatc ggcggaggta taaaagccag tacattcgac aatgatttct
     2941 gcccctttcg ctttccacgg aatattttta gcctcttttt cggcgtaaac cgcgatactt
     3001 ttcccatcaa cgataagtga atcttccgta aaatcaacgc tccaggggaa cggaccatag
     3061 tttgaatcat gtttcagcag ataggcgaga atttttgggg aagtaagatc attaatagcg
     3121 acaacgtcta tgttgctttt gacttcaagt aaacgacgca acaccagtcg accgatacga
     3181 ccaaaaccgt taataccaac tttactcatg gttttctcct gtcaggaacg ttcggatgaa
     3241 aattgatcct ttccaggctt agaccaggat tgtgggatgg gcaatctcca ttcttacagt
     3301 gaaacgtaac gtactgaaaa cgggtgaaca atatttaatg aaattttgag aaaagcccgt
     3361 tatgttaacg gaaaattatg ttaaagcagg aaatgttatg gaaaataaat actcaaggtt
     3421 acaaatcagc attcactggc tggtcttttt gctggttatc gcagcgtatt gcgcaatgga
     3481 gtttcgtggt ttcttcccac gtagcgatcg gccgctcatc aacatgattc atgtttcctg
     3541 tggcatctca attctcgtgc tgatggtggt tcgtcttttg ttaagactga aatacccaac
     3601 cccgccgatt atacctaagc cgaaaccgat gatgacggga ctggcgcatt tgggacattt
     3661 ggtgatttat ctcctgttta ttgcgctgcc agtgattggt ttggtgatga tgtataaccg
     3721 gggcaacccg tggtttgcgt ttggtttgac gatgccttac gcttcagagg ccaatttcga
     3781 acgggttgat agcttaaagt cgtggcatga gacgctggcg aatctgggat attttgtcat
     3841 cgggttgcac gctgcggctg cactggcaca ccactatttc tggaaggaca acacacttct
     3901 acgcatgatg ccgcgtaaac gttcctgaag gatatttaaa gaaaacgcct gtactaaaac
     3961 cgacccgtgg tacaggcgaa gaatacgggt ctacatcgga agcgcctatt atatttattt
     4021 gtatgacaaa taaaaccata atccttgccc atacgtccat ctggcttatt tttaatcaat
     4081 tcacccgatc tttgatctca tcaacggtat caaaataaac cagcgtataa cgttcaaatc
     4141 gcacataaga ttcgataaac catggaggtt atatgaaaaa actggcactt attttgttta
     4201 tgggaacgct tgtttccttt tatgccgatg ccgggcgcaa accctgttct ggttcgaaag
     4261 gggggatctc acactgtacg gcaggcggca aatttgtctg taatgatggt tctattagtg
     4321 catcgaaaaa aacatgcact aactgaagtg taaaaggggt gccatgagaa aatgaatctg
     4381 ctagtcagat gcgcggggaa aatccccgcg cttgccctta cctggacgtg caggccatga
     4441 gcgcagcaac ctccttatca ccgtcccgga accgcagttc gtagagcgtt tgtcgggtca
     4501 ggagtgtgaa tgccagaatc gtaatgcaga taatgagcag acacaccacc agagggttgc
     4561 gcttcatagc ctttctcctt gccggatggc gggtaagagg ctaacaatct gaattgcttg
     4621 gttcattcgt tggcctcggt ttgtactgac cccaaaaagt tggacagtta aacacgaggc
     4681 atataggtct gattccgata ttcatttgga gtcagacctt ttaatttcag gctaattctt
     4741 ctgctgttgt agtattcaat atattccgta acagcatcct tcagttcgct tatattactg
     4801 aactcattag actggccccc tgaatctcca gacaaccagt atcacttatt taagtgatag
     4861 tcttaatact agtt
//
LOCUS       DAEEGA010000190         4867 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEEGA010000190 DAEEGA010000000
VERSION     DAEEGA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.191"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..970
                     /locus_tag="KLH46_004055"
     CDS             <1..970
                     /locus_tag="KLH46_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBE0380520.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1551..2774
                     /locus_tag="KLH46_004056"
     CDS             1551..2774
                     /locus_tag="KLH46_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380521.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            3021..3587
                     /gene="slp"
                     /locus_tag="KLH46_004057"
     CDS             3021..3587
                     /gene="slp"
                     /locus_tag="KLH46_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBE0380522.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            3743..4273
                     /locus_tag="KLH46_004058"
     CDS             3743..4273
                     /locus_tag="KLH46_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0380523.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4315..4867)
                     /locus_tag="KLH46_004059"
                     /pseudo
     CDS             complement(4315..4867)
                     /locus_tag="KLH46_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="MgtC/SapB family protein"
BASE COUNT         1556 a          999 c          905 g         1407 t
ORIGIN      
        1 cgcagagatg tttgtgtttc tcgccgtcga gctttcgttg ctgtttatag tgattagtgc
       61 cggtgtcagc ctgataagac aaaaggtgcc agaccataaa atccagcaga tgatgggggc
      121 cagaaaaggg agaggctatc tcctggctgc tctgttggga gccgttaccc cgttctgtag
      181 ttgctcgaca atacccatgt tacgtggatt gttgtcagcg aaagccgggt ttggtccgac
      241 tctcactttt ttatttgttt caccattact taatcccatt atcgtcggac taatgtggat
      301 gacctttggt tggaaagtca ccttgttgta cgcgattatc gcagccggcg tctccgttct
      361 tgccagtatt atcctggatt ctctggggtt tgaacgtcat atcattgcca gtaaaagctc
      421 atcagcaaat tgttgtgctc cagcaaaaac ttcgccggga acgacatata cgcccataaa
      481 agtgagttgt tgtagtccag cggctaaagc cattgagaac cccgtagtta cctgttgcag
      541 taccaaagct gtagtaagta ttaatcccat aaaactagcc accaaagatg cgttgcaaca
      601 gtttaaagat gtactgccat atcttttgtt aggagtatta ataggatctt ttatttatgg
      661 ctttattccc tcagcgtgga ttgccgctca tgcaggagca gataatccct tcgccatccc
      721 attaagcgcc gttgtcggta ttccgctgta tatccgggct gaggcggtta tccctctggc
      781 atctgttctg atgacaaaag gaatgggtct gggagcatta atggcattaa tcatcggcag
      841 tgccggcgca agcctgacgg aagtgatatt acttaaatca atgttcagaa tgccgatgat
      901 agctgcattc ctgacggtta tattaggtat ggctatcttg atgggctatt tgactcaatt
      961 gctattttaa atagatgcca gcagtaaact ataagatgca tagttagtgc catatccttc
     1021 taagtagaaa atcatagaca ggataatcct taaaaatcac aaatgattta actaatgcat
     1081 tgagaatgat gacggctcgg tcaacaagtc atcaacagac aataaccacg taatcatgct
     1141 tctctgacat tttatcggag aagcaatgac tctttgtctc agtaccctcc tcctttttaa
     1201 aaggcagatt tcagtcgcta atacaacaca tttttaaata cactttaacc tggttcatta
     1261 ttacacatct aaaacatcac aaaaatatta cttcacacac tatcggagaa cataataact
     1321 ttttaaaatg agcgtttttt gatagttgtt aacgttttcc tttacgatac tatcactgcc
     1381 ctttttttat acacaaatat taccgggtgg aaatgaaagg gaatttaaag gtaattatat
     1441 caggaagata taacgcatta tatttattgt gtgtagaaga aaatccattc ctgttctaat
     1501 tatccaattt aaaaacatta gcataaaaca gatgatgaat aaggaatgtt atgtcaattg
     1561 actttacccc aggtataata aatacatatc acggcgatat ttataactgc acaacaaata
     1621 ccgataatgt taaaacgcca gacacaccga agtggccttg tgataattgg gaagaacaac
     1681 aacccattaa ttccacattc tctggagaag gatacaaccc tgaacaattt gatttagcgc
     1741 aacaacaact ccaacaaatt aatgcatgcc acacgaacac cacatataca aatgcagact
     1801 actcaaaagt tgtggttcaa cttgtaagtc ttattaataa cattgaaacg ataagttcga
     1861 caccacttac gcaacagaca caatcaatac ttaatcagat tcataacatt agatacgaaa
     1921 aggataagga ttctgtatgt caaatcattg ttgtcgttaa tccagaaccc aacaatccaa
     1981 tcataactaa aattctcgta gaagaaggaa tacccgaaag attcagtgtc caaaccgttt
     2041 cttccgataa taaaaatttt gctgggcaac gagctgactt accaactgat ataagagaca
     2101 ttcaaagctt gtacttaaaa atggcgaagc tgtatattga gcacagtaaa aatgaaaata
     2161 ggatggaagc ccttgctgga tgcgacttta ttgatttcaa catgacaggg caagatatgt
     2221 ccaagctagt cctaacctta tcaaaatttt attttgaaga tctacttaat ataaatttca
     2281 ctgacgcaaa tttatttaac actatttttt cacacaagga aaatcccatc cccaaactac
     2341 ataaatatga acaacatctt gacaaacaaa tcaatggttt gttttctaca ttgttaacta
     2401 ttaatgacaa ctctttacgc gcgaaagcgg aaattgcttc gagaataatt gatttcttag
     2461 aggcaaaggt agtgaatctt tcatttgatg acatattaaa atataagcaa gagttcaaaa
     2521 aaatatgtta taaacaactt caagagttta caacaccctc tctatacaat aaaattcaaa
     2581 aatgggcaac aatgtctaaa aatgaattca tagcatttca ttacgagaca cttcaacccg
     2641 aaaagatttc ataccccttt tatttaaaga gagatctgcc taatgaaaaa gatataaatt
     2701 atggggtgga aatagaaata ccatctggca aaagaattcg cttatcaaat cattatcaga
     2761 atataatacc ataatattga ttattagcac tttataatca ttgaataaaa atagatttta
     2821 tgtaatttta aaacaaaggg ttatattatt gggcgatgga tactcatacc acgccccaaa
     2881 ataacggaat tgaactaaac agaagggata taaacatcag acaggtttac gttactatca
     2941 ggcatatcac ctcagaatca gatgaaaact ataaagaaat atctattatg gttttaatat
     3001 ttgttgataa ggatagtaac atgaacatga caaaaggtgc actcatcctc agcctttcat
     3061 ttttgcttgc cgcatgtagt tcaattccgc aaaatatcaa aggcaataac caacctgata
     3121 ttcaaaaaag ttttgttgct gttcataacc agccggggtt atatgttggt caacaagcgc
     3181 gctttggtgg gaaggttatc aacgttatca atggcaaaac ggatacgttg ttagaaatcg
     3241 ctgtattacc gttggatagc tatgcgaagc ctgatattga agccaactat cagggccgac
     3301 tgctcgccag acaaagcggc ttccttgatc cagtgaacta tcgtaatcac tttgttacca
     3361 tcctcggtac cattcagggt gaacaaccag gctttatcaa taaagtcccg tataacttcc
     3421 tggaagtgaa tatgcagggc atccaggtgt ggcatttgag agaagtggtt aataccacct
     3481 ataacctgtg ggattacggc tatggtgcat tctggccgga accgggctgg ggtgcgcctt
     3541 actacaccaa tgcggtgagt caggtaacac ctgagctggt caaataacac caccgaaaga
     3601 tgcagatgct ttctcagcat ctgcatcatg tattacataa aattaatata cagtaagcta
     3661 actattatta ttataagccc tgtcctgtta attacctttg gcaaactgat tataaagtta
     3721 atgtccgcac caggagtcgg ttatgtttct tataattacc agggatacga tgttcttcac
     3781 cgcgatgaaa aacattctga gtaaaggtaa tgtcgttcat atacagaacg aagaagagat
     3841 cgacgtaatg ttgcatcaga atgccttcgt cattattgat acattaatga ataatgtatt
     3901 tcattctaat tttctcactc aaattgaacg attaaaacct gtccatgtca ttgttttctc
     3961 cccctttaat attaaacgct gtctggggaa agtaccggtg acctttgttc cgcggactat
     4021 cactatcatt gattttgtcg cactcatcaa tggcagttac tgctctgtgc ctgaagcgaa
     4081 tgtgtcactt tcgcgcaagc aacatcaggt tctgagctgc attgcgaatc aaatgacaac
     4141 tgaagatatt ctggagaaac tgaaaatatc gctaaaaacg ttctactgcc ataaacacaa
     4201 tatcatgatg atcctcaatc ttaagcggat caatgagctg gtacgccatc agcatattga
     4261 ttatctggtg tgaatttcag gcttacggtg agtctggcta cgctgccaca cagattagct
     4321 aattgaaacg cctttcaccc ctgccatacc ttttaataat cgcaacaggt cttctatcaa
     4381 cgttgtggca tgaagctgga tatcaatggc gaccacttca tgatcttcat tttcctgcaa
     4441 cgaaactaaa tcagttttta ttttttgctg cttaaaccag tcgagcattg ataccgtatt
     4501 accgttcact aatgtcagct gtaaatggta atttttgttc atcaggcgga aggttaattg
     4561 atggaagact tccagcacca acaaggtcat cactgaacca taaataccca gttcgtacat
     4621 accgctgcca ataaccatac ctatgcggcg gtcacccaga tatccgctgc cgtcgtcaga
     4681 cctacaatat tttggttacg aacgagaatg ttacctgcac cgataaaccc gacgcccgtc
     4741 accacctgag cagcaatacg gctggggtcg agtccgacgt gatctaaaga cagcacgtca
     4801 gcaaaaccat atttcgaaac aatcataaac agggcgcttc ccatgccaat taatacatgt
     4861 gtgcgta
//
LOCUS       DAEEGA010000191         4865 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEEGA010000191 DAEEGA010000000
VERSION     DAEEGA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.192"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..428
                     /locus_tag="KLH46_004060"
     CDS             <1..428
                     /locus_tag="KLH46_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0380524.1"
                     /translation="NPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPL
                     GVYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSL
                     GTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            762..3041
                     /gene="ydeP"
                     /locus_tag="KLH46_004061"
     CDS             762..3041
                     /gene="ydeP"
                     /locus_tag="KLH46_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBE0380525.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3289..3492
                     /gene="safA"
                     /locus_tag="KLH46_004062"
     CDS             3289..3492
                     /gene="safA"
                     /locus_tag="KLH46_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBE0380526.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3560..4321
                     /gene="ydeO"
                     /locus_tag="KLH46_004063"
     CDS             3560..4321
                     /gene="ydeO"
                     /locus_tag="KLH46_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBE0380527.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4723..>4865
                     /locus_tag="KLH46_004064"
     CDS             4723..>4865
                     /locus_tag="KLH46_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBE0380528.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
BASE COUNT         1391 a         1038 c         1080 g         1356 t
ORIGIN      
        1 gaaatccgcg taattttacg tggaatatta tttcaaataa cagtgttgtt atgccaacag
       61 gcggctgcac cgttgatagt cgtaatgtca ccgtggatct cccggacttt cctggaagcg
      121 cagaaatacc ccttggcgta tattgctcaa gcgaacaaaa gctctcattt tatctttcgg
      181 gtgccaccac tgacagtagc cgacaagtgt ttgctaacac agcacctgat gcaacaaaag
      241 catcgggagt gggtgtcact ctaatgcgta atggaaaaat actggctacg ggagaaaatg
      301 tctccctggg gacagtaaat aaaagcaaag tacctctggg tttaagcgca acctatggac
      361 aaacaggaaa caaggtttca gccggaaccg tccagtcagt cattggtgtc acttttatct
      421 atgaataatt gtgattttga ccttagtcta tgcgctccat tcacgaaaat tgcgcggata
      481 attattttgt gaaggctatt atcctacacc tgtaagaaaa tccgcgcaca attgaagcca
      541 gagtcttatt ttttatattg aaaaatatgt taatttaaac actatgagag atgatgctta
      601 acttatcgta tttaatctat tgtttaacga taatagatta aaaatagaaa gagagtagtc
      661 ccttactctt atatatccat gttggcgata atccctttgt atgtactgtg catcatcgct
      721 attacaaatc ctaataattc atttccacac aggataagta gatgaagaaa aaaattgaat
      781 cctaccaggg tgctgcaggt ggttggggtg ctgttaaatc cgtagcgaat gcagtacgta
      841 agcagatgga tatacgccat gatgttattg ccatgtttga catgaataag ccagagggct
      901 ttgactgtcc gggttgtgca tggccagatc ctaagcacag tgcgtcattc gacatttgtg
      961 aaaacggcgc aaaagcaatc gcctgggaag tcacggataa gcaggtaaat gcctctttct
     1021 ttgctgagaa tacggttcaa tcattactta cctggggaga tcacgagctt gaggctgcgg
     1081 ggcgtctcac tcagcctttg aaatatgatg ccgtcagcga ctgttacaag ccattaagct
     1141 ggcaacaagc tttcgacgaa attggcgcac gccttcaaag ctatagtgat cccaatcagg
     1201 ttgaattcta tacttcgggc cgcacttcca atgaagctgc ctttctttat cagctttttg
     1261 cccgtgaata cgggagcaat aactttcccg actgctccaa catgtgccat gaaccgacaa
     1321 gcgtgggttt ggcagcgagt atcggtgtag gtaaagggac cgtgttgctg gaagactttg
     1381 agaagtgcga tttagtcatt tgcattgggc ataaccctgg tacaaaccac cctcgcatgc
     1441 tgacttcgtt gcgcgcttta gtgaaacggg gagcgaaaat gatcgccatc aatcctctac
     1501 aggaacgtgg cctggagcga tttaccgcac cgcaaaaccc gtttgaaatg ctgacgaact
     1561 ctgagactca gttggccagt gcctactaca acgtgcgcat tggtggcgat atggcgttgc
     1621 tcaaggggat gatgcgcctg ttaattgagc gcgatgatgc tgcaagtgcc gcaggtcggc
     1681 cctcattgct tgatgacgaa tttattcaaa cgcataccgt cggctttgac gagctacgcc
     1741 gtgacgttct caattccgag tggaaagata tcgaacgtat ttctggacta agtcagacac
     1801 aaatcgccga actggctgac gcatatgccg ctgccgaacg caccattatc tgttacggaa
     1861 tggggatcac tcagcacgaa catggtaccc agaacgtgca gcaactggtc aatttgctgt
     1921 tgatgaaagg taacattggc aagcctggtg cgggtatctg cccactacgt ggtcactcta
     1981 atgtacaggg cgaccgaacc gtcggtatca ccgagaaacc gtctgcagag tttctggctc
     2041 gcctgggtga gcgctatggc ttccccccac ctcatgcacc tggacatgct gcaattgcca
     2101 gcatgcaagc aatatgtacg gggcaggctc gagcattgat ctgcatgggg ggcaactttg
     2161 cactggcaat gccagatcgg gaagcgagcg ctgtaccgtt aacgcaatta gatttggcgg
     2221 tacacgtagc cactaagctt aaccgctctc atctgctgac cgcacggcat agctatattc
     2281 tgccggttct ggggcgtagc gagattgata tgcaaaaaag cggtgcgcag gcggtaaccg
     2341 ttgaggattc aatgtcgatg attcatgcct cgcgtggcgt gttaaaaccc gccggtgtaa
     2401 tgctgaaatc agagtgtgca gtggtcgcgg gaatcgcgca ggcagcacta ccccagagcg
     2461 tggtagcctg ggagtatctg gtggaagatt atgatcgcat tcgcaatgac attgaagctg
     2521 tgctgccaga gttcgccgac tataaccagc gcatccgtca tcccggtggt tttcacctga
     2581 taaatgcagc tgctgaaagg cgctggatga cgccgtcagg taaggctaat ttcattacca
     2641 gcaaagggct gttagaagat ccctcttcag cgtttaacag taagctggtc atggcgacag
     2701 tacgcagcca cgatcagtac aacacgacga tttatggtat ggatgatcgc tatcgagggg
     2761 tattcggtca acgagatgtg gtctttatga gtgctaaaca agctaaaatt tgccgtgtaa
     2821 aaaacggcga aagagttaat cttattgcgc ttacgccaga cggtaagcgc agctcacgcc
     2881 gcatggatag attaaaagtg gtcatttacc ctatggctga ccgctcactg gtgacctatt
     2941 ttccagaatc gaatcacatg ctaacacttg ataaccacga tccattaagt ggcattcctg
     3001 gctataaaag tattccggtt gaattagaac catcaaatta atgcctcttc tcatttcttc
     3061 tgctgtcatc cgcacagcag aagaattcct cattgactat tattgcgcaa tttgctcaca
     3121 tggattaaat taaactacat actataagat ataaacttct gcctacagct gtaagaaact
     3181 ccgctcagta ctgaagcacc agtcctattt cttcttttct ccagcctgtt atattaagca
     3241 tactgattaa cgacttttaa cgttatccgc taaataaaca tatttgaaat gcatgcgacc
     3301 acagtgaaaa acaaaatcac gcaaagagat aactataaag aaatcatgtc tgcaattgtg
     3361 gttgtcttat tactgacact tacgttgata gccatttttt cggcaattga tcagctgagt
     3421 atttcagaaa tgggtcgcat tgcaagagat cttacacatt tcattatcaa tagtttgcaa
     3481 gactggaaat aaacagcaaa ttataaatgt gaaatgttta aaaggtatcg ataaaacttt
     3541 attgttttaa ggagataaaa tgtcgctcgt ttgttctgtt atatttattc atcatgcttt
     3601 caacgctaac attttagata aagattacgc cttctccgac ggcgagatcc tgatggtaga
     3661 taacgctgtt cgtacacatt ttgaacctta tgagcggcat tttaaagaaa tcggatttaa
     3721 tgaaaatacc attaaaaaat atctacaatg cactaacatc cagacagtga cggtgcctgt
     3781 tcctgcgaag tttttacgtg cttcaaatgt accgactgga ttgcttaatg aaatgattgc
     3841 ttatctcaac tcggaagaac gcaatcatca taatttttca gaacttttgc ttttttcttg
     3901 cctgtctatt tttgccgcat gcaaaggttt cattacacta ttaactaacg gtgtgctatc
     3961 cgtttccggg aaagtgagaa atattgtcaa catgaagctg gcgcacccat ggaagctgaa
     4021 agatatttgt gactgcctgt acatcagtga aagcctgttg aagaaaaaac taaagcaaga
     4081 gcaaacgaca ttctcacaga ttctattaga tgccagaatg cagcacgcaa aaaatttgat
     4141 acgcgtagaa ggttcagtca ataaaattgc cgaacaatgt ggttatgcca gtacatctta
     4201 ttttatttat gcgttccgca aacatttcgg caacagtccg aagagagttt ctaaggagta
     4261 ccgttgtcaa aggcacacgg gtatgaatac gggcaacacg atgagtgctt tagctatttg
     4321 attatttgct aacgagtagt caaccacgca cgctacgtaa gaattatatg gggcagccat
     4381 tccctgcccc gcgttgtttt taggcgatat atttattgaa ataaataagt gacatccatc
     4441 acatatttat gcacttgcat aacctgttgc atgattattt atgatctcaa ttcggcattt
     4501 tgtcagtaaa atgcaataat ttattaaata tcaataaatt agttgtttat cggcgagaaa
     4561 ttacttaata gaacagaaag taatgtcaac gctttatgga ctgttatttc ccttttttta
     4621 gctaaatctg ctatctcttt atgtgactaa cttcacttac atccacttat ttctcttcgt
     4681 aaaattactt cggaattaag tacaataaga agaggaacat ttatgaagtc tgcattaaag
     4741 aaaagtgtcg taagtacctc gatatctttg atactggcat ctggtatggc tgcatttgct
     4801 gctcatgcgg cagatgatgt aaagctgaaa gcaaccaaaa caaacgttgc tttctcagac
     4861 tttac
//
LOCUS       DAEEGA010000192         4636 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEEGA010000192 DAEEGA010000000
VERSION     DAEEGA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.193"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..311
                     /locus_tag="KLH46_004065"
     CDS             <1..311
                     /locus_tag="KLH46_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBE0380529.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="KLH46_004066"
     CDS             468..1217
                     /locus_tag="KLH46_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBE0380530.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="KLH46_004067"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="KLH46_004067"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HBE0380531.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="KLH46_004068"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="KLH46_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBE0380532.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLMSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="KLH46_004069"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="KLH46_004069"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBE0380533.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="KLH46_004070"
     CDS             4084..4557
                     /locus_tag="KLH46_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBE0380534.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1238 a         1162 c         1138 g         1098 t
ORIGIN      
        1 cgctggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agcctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcatcagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaat
//
LOCUS       DAEEGA010000193         4610 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEEGA010000193 DAEEGA010000000
VERSION     DAEEGA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.194"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            224..2647
                     /gene="pgaA"
                     /locus_tag="KLH46_004071"
                     /pseudo
     CDS             224..2647
                     /gene="pgaA"
                     /locus_tag="KLH46_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            2656..4149
                     /gene="pgaB"
                     /locus_tag="KLH46_004072"
     CDS             2656..4149
                     /gene="pgaB"
                     /locus_tag="KLH46_004072"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HBE0380535.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNHLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIR"
     gene            4153..4610
                     /gene="pgaB"
                     /locus_tag="KLH46_004073"
                     /pseudo
     CDS             4153..4610
                     /gene="pgaB"
                     /locus_tag="KLH46_004073"
                     /inference="COORDINATES: protein motif:HMM:NF026233.1"
                     /note="in Yersinia this protein is involved in biofilm
                     formation and hemin adsorption; related protein PgaB in
                     Escherichia coli is an outer membrane N-deacetylase
                     involved in poly-beta-1,6-N-acetyl-D-glucosamine (PGA)
                     export; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase"
BASE COUNT         1317 a         1033 c         1061 g         1199 t
ORIGIN      
        1 tttatgccgt attcctgaag atcctcatca ttggaatgga ttttcgggcg agaaaaggat
       61 tttatatgga cactctgctc atcatttctt cttctcatca tcaacaattc acgtctctct
      121 tccgcgttta ataacggatt atgaggtgca aaaatatctt tcttttcagt tacctgtaat
      181 tagatacaga gagagatttt ggcaatacat ggagtaatac aggatgtatt caagtagcag
      241 aaaaaggtgc ccgaaaacca aatgggcttt gaaacttctt actgccgcat ttttagcagc
      301 gagtcccgcg gcgaagagtg ctgttaataa cgcctatgat gcattgatta ttgaagctcg
      361 caagggtaat actcagccag ctttgtcatg gtttgcacta aaatcagcac tcagcaataa
      421 ccaaattgct gactggttac agattgcctt atgggccggg caagataaac aggttattac
      481 cgtttacaac cgctaccgtc atcagcaatt accagcgcgt ggttatgcag ctgtcgccgt
      541 cgcttatcgt aacctgcaac aatggcaaaa ctcgcttaca ctgtggcaaa aggcgctctc
      601 tctggagccg caaaataagg attatcaacg gggacaaatt ttaaccctgg cagatgcggg
      661 tcactatgat actgcgctgg ttaaacttaa gcagcttaac tctggagcac cggacaaagc
      721 caatttactc acagaagcct atatctataa actggcgggg cgtcatcagg atgaattacg
      781 ggcgatgaca gagtcattac ctgaaaatgc atctacgcaa caatatccca cagaatacgt
      841 gcaggcatta cgtaataatc aacttgctgc cgcgattgac gatgccaatt taacgccaga
      901 tattcgcgct gatattcatg ccgaactggt cagactgtcg tttatgccta cgcgcagtga
      961 aagtgaacgt tatgccattg ccaatcgcgc cctcgcccaa tacgctgcat tagaaattct
     1021 gtggcacgat aacccagacc gcactgccca gtaccagcgt attcaggttg atcatcttgg
     1081 cgcgttatta actcgcgatc gttataaaga cgtcatttct cactatcagc gattaaaaaa
     1141 gacggggcaa attattccgc cctaggggca atattgggtt gcatcggctt atctcaaaga
     1201 tcatcagccg aaaaaagcac agtcaataat gaccgagctc ttttatcaca aggagaccat
     1261 tgccccggat ttatccgatg aagaacttgc ggatctcttt tacagccacc tagagagtga
     1321 aaattatccg ggcgcgctaa ctgtcaccca acataccatt aatacttcgc cgcctttcct
     1381 tcggttaatg ggcacgccta cgagcatccc gaatgatacc tagttacagg ggcattcgtt
     1441 tctctcaacc gtagcaaaat atagtaatga tcttcctcag gctgaaatga cagccagaga
     1501 gcttgcttat aacgcaccag gaaatcaggg actgcgcatt gattacgcga gtgtgttaca
     1561 agcccgcggt tggcctcgtg cagcagaaaa tgaattaaaa aaagcagaag tgatcgagcc
     1621 acgtaatatt aatctggagg ttgaacaagc ctagacagca ttaacgttac aagaatggca
     1681 gcaggcagct gtcttaacgc acgatgttgt cgaacgtgaa ccgcaagatc ccggcgttgt
     1741 acgtttaaaa cgtgcggttg atgtacataa tcttgcagag cttcgtatag ctggttcaac
     1801 aggaattgat gccgaaggcc cggatagtgg taaacatgat gtcgacttaa ccaccatcgt
     1861 ttattcacca ctgctgaagg ataactggcg cggttttgct ggattcggtt atgccgatgg
     1921 acaatttagc gaaggaaaag ggattgttcg cgactggctg gcgggtgttg agtggcggtc
     1981 acgtaatatc tggctcgagg cggagtacgc tgaacacttt ttcaatcatg agcataaacc
     2041 cggcgcgcgc ctgtctggct ggtatgattt taatgataac tggcgtattg gttcgcaact
     2101 ggaacgcctc tctcaccgcg ttccattacg ggcaatgaaa aatggtgtta caggcaacag
     2161 tgctcaggct tatgttcgct ggtatcaaaa tgagcggcgt aagtacggtg tctcctgggc
     2221 tttcactgat ttttccgaca gtaaccagcg tcatgaagtc tcaattgagg gtcaggaacg
     2281 catctggtct tcaccatatt tgattgtcga tttcctaccc agtctgtatt acgaacaaaa
     2341 tacagaacac gataccccat actacaaccc tataaaaacg ttcgatattg ttccggcatt
     2401 tgaggcaagt catttgttat ggcgaagcta tgaaaatagc tgggagcaaa tcttcagcgc
     2461 aggtgttggt gcctcctggc aaaaacatta tggcacggat gtcgtcaccc aactcggcta
     2521 cgggcaacgc attagctgga atgacgtgat tgatgctggc gcaacgctac gctgggaaaa
     2581 acgaccttat gacggtgaca gagaacacaa cttatacgtt gaattcgata tgacattcag
     2641 attttaagga taaatatgtt acgtaatgga aataaatatc tcctgatgct ggtgagtata
     2701 attatgctca ccgcgtgcat cagccagtca agaacatcat ttataccgcc acaggatcgc
     2761 gaatctttac tcgccgagca accgtggccg cataatggtt ttgtggcgat ttcatggcat
     2821 aacgttgaag acgaagctgc cgaccagcgt tttatgtcag tgcggacatc agcactgcgt
     2881 gaacaatttg cctggctgcg cgagaacggt tatcaaccgg tcagtattgc tcaaattcgt
     2941 gaagcacatc gaggaggaaa accgctaccg gaaaaagcag tagtgctgac ttttgatgac
     3001 ggctaccaga gtttttatac ccgcgtcttc ccaattcttc aggccttcca gtggcctgct
     3061 gtatgggccc ccgtcggcag ttgggtcgat acgccagtgg atgaacaagt aaaatttggc
     3121 gatgagatgg tcgatcgaga atattttgcc acgtggcaac aagtgcgaga agttgcgcgt
     3181 tcccggctcg ttgaggtcgc ttctcataca tggaattctc actacggtat tcaggctaat
     3241 tccaccggca gcttaatgcc tgtatatgta aatcgtgcat attttactga ccacgcacgg
     3301 tatgaaaccg cagcagaata ccgggaaaga attcgtctgg atgctgtaaa aatgacggaa
     3361 tacctgcgta caaaggctga ggtaaatcca cacgtttttg tttggcctta tggcgaagcg
     3421 aatggcatag cgatagagga attaaaaaaa ctcggttatg acatgttttt cacccttgaa
     3481 tcaggtttgg caaatgcgtc acaattggat tccattccgc gagtattaat cgccaataat
     3541 ccctcattaa aagagtttgc ccagaaaatt attaccgtac aggaaaaatc accacaacgg
     3601 ataatgcata tcgatcttga ttacgtttat gacgaaaacc gccagcaaat ggatcgcaat
     3661 attgatgtgc taattcagcg ggtgaaagat atgcaaatat caaccgtgta tttgcaggca
     3721 tttgctgatc ccgatggtga tgggctggtc aaagaggtct ggtttccaaa tcatttgcta
     3781 ccaatgaaag cagatatttt tagtcgggtt gcctggcaat tacgtacccg ctcaggtgta
     3841 aacatctatg cgtggatgcc ggtattaagc tgggatttag atcccacatt aacgcgagta
     3901 aaatacttac caacagggga gaaaaaagca caaattcatc ctgaacaata tcaccgtctc
     3961 tctcctttcg atgacagagt cagagcacaa gttggcatgt tatatgaaga tcttgccgga
     4021 catgctgctt ttgatggcat attgttccac gatgatgctt tgctttcaga ttatgaagat
     4081 gccagtgcac cggctatcac ggcttatcag caagcaggct ttagcgggag tctgagcgaa
     4141 attcgataaa acccggagca atttaaacag tgggcccgct ttaaaagtcg tgcgttaact
     4201 gacttcactt tagaacttag tgcgcgcgta aaagccattc gcggtccaca tattaaaact
     4261 gcacgaaata tttttgcact tccggtaata caacctgaaa gtgaagcctg gtttgcacag
     4321 aattatgctg atttcctaaa aagctatgac tggaccgcta ttatggctat gccttatatg
     4381 gaaggtgtcg cagaaaaatc ggctgaccaa tggttaatac aattgaccaa tcaaattaaa
     4441 aacatccctc aggctaaaga caaatctatt ttagaattac aggcacaaaa ctggcagaaa
     4501 aatggtcagc atcaggctat tccttcgcaa caactcgctc actggatgag cctattacaa
     4561 ctgaatggag tgaaaaacta tggttattat cccgacaatt ttctgcataa
//
LOCUS       DAEEGA010000194         4577 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEEGA010000194 DAEEGA010000000
VERSION     DAEEGA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.195"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..357
                     /locus_tag="KLH46_004074"
     CDS             <1..357
                     /locus_tag="KLH46_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBE0380536.1"
                     /translation="VFVHTKPAYRSDGIKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            619..981
                     /locus_tag="KLH46_004075"
     CDS             619..981
                     /locus_tag="KLH46_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBE0380537.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYTAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            978..1898
                     /locus_tag="KLH46_004076"
     CDS             978..1898
                     /locus_tag="KLH46_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBE0380538.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGAEMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPKYILKKGRK"
     gene            1901..2852
                     /locus_tag="KLH46_004077"
                     /pseudo
     CDS             1901..2852
                     /locus_tag="KLH46_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI"
     gene            2879..3119
                     /locus_tag="KLH46_004078"
                     /pseudo
     CDS             2879..3119
                     /locus_tag="KLH46_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060423.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI domain protein"
     gene            3116..3421
                     /locus_tag="KLH46_004079"
     CDS             3116..3421
                     /locus_tag="KLH46_004079"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380539.1"
                     /translation="MTVNWLSGGDIQITPVFFNADSGKINFTGVRQQTLASWHSKEAF
                     NSAPERQFIAVSIANEPERCKEMTSCLAGIQEQLGKPDEVLNFEGRVILVFNKKLNL"
     gene            complement(3655..4095)
                     /locus_tag="KLH46_004080"
     CDS             complement(3655..4095)
                     /locus_tag="KLH46_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HBE0380540.1"
                     /translation="MRITLQWDINEMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINSQSVTKMITCGENGHPVIVDRPSPTPEQLALINNEKKSALIAEATNVIAPLQ
                     DAVDLDMATDDETKLLLAWKKYRALLMRVDIKNTEWPKKPESNL"
     gene            complement(4067..>4577)
                     /locus_tag="KLH46_004081"
     CDS             complement(4067..>4577)
                     /locus_tag="KLH46_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380541.1"
                     /translation="CSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVK
                     TALENLGLGEAAKRNVGTGANQIPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPN
                     VSSTYGMRFPMTFSRVLAVTVTLNVTGASGQPTVSATNVQNTGFDITVSPGSGYGSSA
                     DAYYIAMGY"
BASE COUNT         1308 a          937 c          901 g         1431 t
ORIGIN      
        1 gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa taaaaacagc agcggtaagg
       61 aagatgagaa ccgacagcaa caaggcatgg aagggagcgt taaagcgggc aggcattagc
      121 aacttccgct tccatgacct gaggcatacc tgggcaagct ggctggttca gtccggtgtc
      181 tctcttcttg cgcttaaaga gatgggagga tgggaaactc tcgaaatggt tcaaagatac
      241 gcccaccttt cagccgggca tctcaccgag cacgcgagca aaatcgatgc gattataagt
      301 cgcaatggca caaatacggc acaagaggag aacgtagttt acttaaatgc gaggtaactt
      361 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaattat
      421 aagaatccgc ttgtaattca aaggattatc ccatcaacac tgcgctcaca cgtcccacca
      481 catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct tatgtgtctc agttttgtct
      541 catcagacat agcaagtatc gatcaattga gacttggatg atagacttca tgcctttcag
      601 agctcattga ttaaataaat gttaaagtta tttgtaaagt acacatctat aggtgtgctg
      661 aacacactta tacactgggt ggtttttggt gtatgtatct ataccgcaca taccagtcag
      721 gctctggcaa acttcacagg tttcgtagtg gctgtgagct ttagcttctt cgcgaatgca
      781 agattcacat tcaaagcatc gactacagcg atgcgctaca tgttatacgt gggatttatg
      841 ggaatattga gtgtgattgt tggttgggca gctgataaat gctcacttcc tccaatagtc
      901 actcttatca ccttctccgc catcagtctg gtgtgcggtt tcgtctattc aaagttcatt
      961 gtctttaggg atgcgaaatg aaaatatctc ttgtcgttcc tgtcttcaat gaagaagaag
     1021 cgatacctgt tttctataaa acggtacgtg aattccaaga gttgaagcca tatgaagtag
     1081 aaattgtatt cataaatgac ggaagtaaag atgccacaga gtcaattatt aacgcgctgg
     1141 ctgtttcaga cccgctagtt gttccgctgt catttacacg caactttggt aaagaaccag
     1201 ccttatttgc agggttagac catgcaagcg gcgatgctgt aattcctatt gatgtcgacc
     1261 tgcaagaccc aattgaggtt atccctcatc ttattgaaaa gtggcaggca ggtgctgaga
     1321 tggtgcttgc taaacgttca gaccgctcaa ctgatggacg actgaaacgt aagacagctg
     1381 agtggttcta taaattacac aacaaaataa gcaccccaaa gatcgaggaa aatgtcggag
     1441 attttcgact catgtctcgt gaggttgtgg agaacattaa actgttgcct gagcgcaatc
     1501 ttttcatgaa aggcatactg agctgggtgg gtggtcagac ggatgtcgtt gaatatgtac
     1561 gcgcagagcg tgttgctggc atctcaaaat ttaatggctg gaaattatgg aatctggcac
     1621 tggaaggtat cacaagtttt tcaacctttc ctcttcgcgt atggacttat ataggcttgt
     1681 ttgttgcaag catttcattt ttatatggtg catggatgat tatagacacc cttgtctttg
     1741 gtaacccagt acgcgggtat ccctccctgc ttgtatcaat acttttcttg ggtggagtgc
     1801 aactgatcgg gattggtgtt ctcggtgaat atataggtag aatttactta gaaacaaaat
     1861 caagacctaa gtatattttg aagaaaggta gaaaataacc atgagcatat gtattaaaca
     1921 atcagcatta aaaatattat tggctttatc agcactccta ataacttggt tgactaccag
     1981 atactttcca gttgaaccgg atgttgctaa cagcccaatt gtatggaggc atattttaga
     2041 gaatggtata tcatcaatcc acgactggaa accaaccgtg gataattggt atttcaccgt
     2101 ttacccaatt aactttttat tttatatgct gttaggtgat gatggcttag tagcattaag
     2161 attatcaact gcagttttct ctattgctat agtaatagca gcgatgttaa cgctacgtaa
     2221 ggcattcggg tttacccctg ctttattttc tattatactt ctatctctaa taccatattt
     2281 ttcatacact tatggttttg tatcacatcc tttttctcat aattcaacaa acgctttcgg
     2341 ctttttatgc ttgcttatat ctgttttcaa catacagtat aaaaatatat ttatcactct
     2401 cctcttaagt ttgacagcat tattttccag cgtatccgat ccgtggttta cagctgcatt
     2461 ttttattcca ctattaatat cttacttttt attctctgta tgggataaaa aattatttaa
     2521 gcatactgca ttaatactat ttgcatgttt aatctctctt tccaatgtat tgcaaaatct
     2581 acttaacata cctccccacc aatttgaaat tgtatcactc aatgatatga ttttaaatgc
     2641 aaaatggtgc attctgttaa taggaaaaag tcttaattta ttagttgtcg acaataacgc
     2701 aacatcttac gcctcatttg tcatttggtt cattgccatt attacatctg cgtggtttgt
     2761 tttatcggac aataaaaaaa acacataccg tatttatatt gtgttatttt cattattatc
     2821 tatcgcaggt attgtctcat cattcatact gagctataaa tcccctgatt atattagcat
     2881 gcgtttcttt atgaatgtta cctgtttcgc actgatattg tgctgtatcg gtacatcaac
     2941 aaaagcaaag atattatttt atctaatcgc atttttattt tcgatcagtt caattaaatc
     3001 ttacacaaat aatgcttcac cattacacga tcaggagaag attgttaaat catacattga
     3061 ttttttaaaa aaaaataatc tccattatgg gtacggctct ttttgggatt tgtcaatgac
     3121 tgtaaattgg ttatctggtg gggacataca gataactcct gttttcttta atgctgattc
     3181 ggggaaaatt aactttactg gcgtgagaca acagacactg gcttcttggc atagcaagga
     3241 ggcgttcaat tctgctcctg agcgacagtt tatagcagtt tccatagcta acgaacctga
     3301 acgatgtaaa gaaatgacat cctgccttgc aggtattcaa gaacaattag gtaagcctga
     3361 cgaagttttg aactttgaag gaagagtaat attagtattt aacaaaaaac ttaacctata
     3421 gtaaattgcc ccctaccata taaaccacac taacgaatga ataacccgtc tttttcgacg
     3481 ggttatatcc ctatcaggaa taaaaatgga gtggttgccg tatacaacaa accctcattt
     3541 agagctatat tctagagtat atgagatact ataaagttga aggagctatc attagaaaaa
     3601 ccatatgctg ttataattag atgtctcgct tttcatccaa taacgaattt tctcttatag
     3661 attgctctct ggttttttcg gccactctgt attttttata tcaacacgca tcaatagtgc
     3721 gcgatatttt ttccatgcca gtaacagttt cgtttcatca tctgttgcca tatctaaatc
     3781 aaccgcatcc tgaagcggcg ctattacatt cgttgcctct gctatcagtg cagatttctt
     3841 ttcattattt attaaggcta attgttctgg tgttggagaa ggacggtcaa caatgacagg
     3901 gtgaccattc tcaccacagg taatcatttt tgttacactc tgcgaattaa tcagtgtttt
     3961 ccactctgat tccgagattt ccactgcatc atcaggaata ttggtgccgt gaaattcagt
     4021 cgaataaaat ccgtttgtcg atgcagaata aaaatatgac atttcgttaa tatcccattg
     4081 caatgtaata cgcatcagca gatgaaccat atcctgaacc gggcgacact gtaatatcaa
     4141 atccagtgtt ctggacattt gtcgccgata cagtcggctg ccctgacgcg ccagtaacgt
     4201 tcaatgtaac tgtgacagca aggactcttg agaatgtcat cgggaacctc atcccgtatg
     4261 ttgaagatac attaggggag ccattcaact gaccgcactg taaaattaat ccagacggaa
     4321 atttttgcgc agcagccgtt gcggtattac ttgacgcgaa caggctcata tcaggtatct
     4381 gattcgcccc tgtacctaca ttccttttag ctgcttctcc caaaccaagg ttttcgagag
     4441 ccgttttcac cgtgccatcc gatttgatat cgccaaacgg attcttgcgg cttaacagca
     4501 gcgcgcgaag tgcggtaagc aactgatcgt gccgcccctt ctccaggctg gcaccggatg
     4561 cctccaccac gctgcaa
//
LOCUS       DAEEGA010000195         4572 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEEGA010000195 DAEEGA010000000
VERSION     DAEEGA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.196"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(129..410)
                     /locus_tag="KLH46_004082"
     CDS             complement(129..410)
                     /locus_tag="KLH46_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBE0380542.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(672..1781)
                     /gene="apbC"
                     /locus_tag="KLH46_004083"
     CDS             complement(672..1781)
                     /gene="apbC"
                     /locus_tag="KLH46_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBE0380543.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1913..3946
                     /gene="metG"
                     /locus_tag="KLH46_004084"
     CDS             1913..3946
                     /gene="metG"
                     /locus_tag="KLH46_004084"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBE0380544.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4087..4278
                     /locus_tag="KLH46_004085"
                     /pseudo
     CDS             4087..4278
                     /locus_tag="KLH46_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215564.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            4342..>4572
                     /locus_tag="KLH46_004086"
     CDS             4342..>4572
                     /locus_tag="KLH46_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0380545.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTLAELESEILQLRKALNEA"
BASE COUNT         1187 a         1137 c         1129 g         1119 t
ORIGIN      
        1 aatatttatc acatctatta attaatgtag atttatgtaa atggtattgt tgatatcaat
       61 aaaaaagacg aaatcattta tataaatgat ttcgtctttc taaatgcaat taaaatcagg
      121 ttgccaaatt aatcgtagcg atttaacacg attcgacgtt tgctatcgct acctgcaaca
      181 accacttctg tcgtaacacc tttgacgggc gaagaaacct gatgacgggt gtcaacatta
      241 tacgttaact gaccacaatg ctgttgtggt tgactattaa ccatagccat actgcatgtt
      301 gggggactga tcctgccatt aatcgttaat gtggcagtct ctgaagagac tgcgggagaa
      361 gccaatccat aggcaagaaa aataattcct gacagccaat acttattcat tgaacgttat
      421 ccctgtagta aaggttatgc ctggatagaa tgagtgcata acaaactata gctgtacatc
      481 cactacacag cacgaaggat gataatgaag cattgcctgt acgatcactc gactttgtag
      541 agtttcggac gaaggtccgc agaatattcg cagtattaaa taagtgttca ttaatgcggc
      601 aaaaacaaat acaccgcatc aatataacat ctctgcggtg tatgaagaat aaccagaatg
      661 gttaataggc gttagaccgc gcggaaggaa atctcgccag gaatgacttc accctgccag
      721 tagagctgag ctgcaacgcg gtcagccagc tggcggtaga tagcggtaaa ttcgctctcc
      781 ggacggctga taaccgttgg cgtgccttta tcgagatcct cacgtaagga gatgtgtaac
      841 ggcatctgcc ccagcagctg tgtatggtat ttttcagcca gtttctctgc accaccggta
      901 ccgaaaatcg gttcatggtg accacagtta ctgcaaatat gcacactcat gttctcgaca
      961 ataccgagca ccggaacctc gactttctcg aacatcacaa tgcctttttt cgcatcgatc
     1021 aacgcgatgt cctgcggggt ggtgaccacg actgcaccag ttactggaat gttttgcgcc
     1081 agcgtcaatt gaatgtcacc ggtgcctggc ggcatatcaa gcaccagata gtcgagatcc
     1141 ggccacaagg tttcctgcaa catctgcatt aacgccttgc tcgccatcgg tccacgccac
     1201 accatcgcat tgtcgtcagt gaccagataa ccaattgaat tggttgccag gccatgagac
     1261 ataatcggtg ccatgtgggt accgtccggc gaagttggac gctgattttc tgcgcccagc
     1321 atggtgggaa ttgatgggcc gtagatatcg gcgtccagaa tgccgacttt cgccccttcc
     1381 gccgccagtg ccagcgccag gtttaccgcc gtggacgatt tacccacgcc gcctttaccg
     1441 gaactcaccg cgataatgtt cttcacaccg ttaattcccg gctgattctt cacgcgtttc
     1501 agagtggcta tgttatgcga cagtttccag tcgatagctt tcgccccggt gatgcgcagc
     1561 agttcggcgc tacattgctc ttttaactct tcgaacgcgc tatgccagac aaacggcata
     1621 accagttcca catgcaacgt gtcgtccatc caggcgacat ggtgcaacgc tttcagcgtg
     1681 gtgaggttat gcttcagggt tgggtgctga aaattggcca gtgtcccggc gaccattgcg
     1741 cgcaaggctt ctggtgattt ggcctgggat tgttcgttca tcccgactcc ttttttgtat
     1801 agataaacca tcagctgata gtttacctga agaatataga gaagtactta cttaacattt
     1861 tcccatttgg tactatctaa ccccttttca ctattaagaa gtaatgccta ctatgactca
     1921 agtcgcgaag aaaattctgg tgacgtgcgc actgccgtac gctaacggct caatccacct
     1981 cggccatatg ctggagcaca tccaggctga tgtctgggtc cgttaccagc gaatgcgcgg
     2041 ccacgaggtc aacttcatct gcgccgacga tgcccacggt acaccgatca tgctgaaagc
     2101 tcagcagctc ggtatcaccc cggagcagat gattggcgaa atgagtcagg agcatcagac
     2161 tgatttcgca ggctttaaca tcagctatga caactatcac tcgacgcaca gcgaagagaa
     2221 ccgccagttg tcagaactta tctactctcg cctgaaagaa aacggtttta ttaaaaaccg
     2281 caccatctct cagctgtacg atccggaaaa aggcatgttc ctgccggacc gttttgtgaa
     2341 aggcacctgc ccgaaatgta aatccccgga tcaatacggc gataactgcg aagtctgcgg
     2401 cgcgacctac agcccgactg aattgatcga gccgaaatcg gtggtttctg gtgctacacc
     2461 agtgatgcgt gattctgaac acttcttctt tgatctgccc tctttcagcg aaatgctgca
     2521 ggcatggacc cgcagcggtg cgttgcagga gcaggtggca aacaaaatgc aggagtggtt
     2581 tgaatccggc ctgcaacagt gggatatctc ccgcgacgcg ccatactttg gttttgaaat
     2641 tccgaacgca ccgggcaaat atttctacgt ctggctggac gcaccgattg gctacatggg
     2701 ttctttcaag aatctgtgcg acaagcgcgg cgacagcgta agcttcgatg aatactggaa
     2761 gaaagactcc accgccgagc tgtaccactt catcggtaaa gatatcgttt acttccacag
     2821 cctgttctgg cctgccatgc tggaaggcag caacttccgc aagccgacca acctgtttgt
     2881 tcatggctat gtgacggtga acggcgcgaa gatgtccaag tctcgcggca cctttattaa
     2941 agccagcacc tggctgaatc attttgacgc tgacagcctg cgttactact acactgcgaa
     3001 actctcttcg cgcattgatg atatcgatct caacctggaa gatttcgttc agcgtgtgaa
     3061 tgccgatatc gtgaacaaag tggttaacct ggcctcccgt aatgcgggct ttatcaacaa
     3121 gcgttttgac ggtgtgctgg caagcgagct ggctgaccct gagctgtaca aaacctttac
     3181 cgatgccgct gaagtgattg gtgaagcgtg ggaaagccgt gaatttggta aagcagtgcg
     3241 cgaaatcatg gcactggctg acctggctaa ccgctatgtc gacgagcagg ctccgtgggt
     3301 agtcgcaaaa caggaaggtc gcgatgctga cctgcaggcg atttgctcaa tgggcatcaa
     3361 cctgttccgc gtgctgatga cttacctgaa gccggtactg ccgaaactga ccgagcgtgc
     3421 agaagccttc ctcaatacgg aactgacctg ggatggtatc cagcaaccgc tgctgggcca
     3481 caaagtgaat ccgttcaagg cgctgtataa ccgcatcgat atgaaacagg ttgaagctct
     3541 ggtggaagcc tctaaagaag aagtgaaagc cactaccgcg ccagttactg gcccactggc
     3601 agatgatccg attcaggaaa ccatcacctt tgacgacttc gccaaagttg acctgcgcgt
     3661 ggcgctgatc gaaaacgcag agtttgttga aggttctgac aaactgctgc gcctgacgct
     3721 ggatctcggc ggtgaaaaac gcaatgtctt ctccggtatt cgttctgctt acccggatcc
     3781 gcaggcactg attggtcgtc acaccattat ggtggctaac ctggcaccac gtaaaatgcg
     3841 cttcggtatc tctgaaggca tggtgatggc tgccggtcct ggcgggaaag atattttcct
     3901 gctaagcccg gatgccggtg ctaaaccggg tcatcaggtg aaataatccc ccttcaaggc
     3961 gctgcatcga cagcgccttt tctttataaa ttcctaaagt tgttttcttg cgattttgtc
     4021 tctctctaac ccgtataaat actggtagca tctgcattca actggataaa attacaggga
     4081 tgcagaatga gacactttat ctatcaggac gaaaaatcac ataaattctg gacggtggag
     4141 caacagggaa acgagttgca tatcagttgg ggaaaagttg gcaccaaagg gcaaagtcag
     4201 ataaaaagtt tttcagatgc tgcggcggcg gaaaaagcgg aactgaagct gattgcggag
     4261 aaggtgaaga aggggtatga ggtagcctga gtttaacgga cactccttcc tgaaatagaa
     4321 tggcatcaga aggagctaat aatgagcaga aaaacccaac gttactctaa agagttcaaa
     4381 gccgaagctg tcagaacggt tcttgaaaat caactttcga tcagtgaagg cgcctcccga
     4441 ttatctcttc ctgaaggcac tttaggacaa tgggttaccg ccgccagaaa agggctcggt
     4501 actcctggtt cccgcacgct ggctgaactg gaatctgaaa ttctgcaact gcgtaaggcg
     4561 ttaaatgaag ct
//
LOCUS       DAEEGA010000196         4557 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEEGA010000196 DAEEGA010000000
VERSION     DAEEGA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.197"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            291..851
                     /locus_tag="KLH46_004087"
     CDS             291..851
                     /locus_tag="KLH46_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HBE0380546.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGL
                     ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILS
                     LAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALMNKVS
                     ENTKQRNLFSKKMTLCISLFLFLGGR"
     gene            794..1183
                     /locus_tag="KLH46_004088"
                     /pseudo
     CDS             794..1183
                     /locus_tag="KLH46_004088"
                     /inference="COORDINATES: protein motif:HMM:NF028467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
     gene            1163..1720
                     /locus_tag="KLH46_004089"
                     /pseudo
     CDS             1163..1720
                     /locus_tag="KLH46_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            1818..3427
                     /locus_tag="KLH46_004090"
                     /pseudo
     CDS             1818..3427
                     /locus_tag="KLH46_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3528..4019)
                     /locus_tag="KLH46_004091"
     CDS             complement(3528..4019)
                     /locus_tag="KLH46_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HBE0380547.1"
                     /translation="MTQRYFLFSGIILCAFIAAILSHIAFHHSNEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKMGFIRRYVSFTYKKFRDTVYL
                     TTVKIQKIQKDDNISDEILENITSDFFIKADTSINYYVTRQNDYNYIFSTGRTPRFIC
                     ITH"
     gene            complement(4024..4200)
                     /locus_tag="KLH46_004092"
     CDS             complement(4024..4200)
                     /locus_tag="KLH46_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE0380548.1"
                     /translation="MLSPIIEKLQKMEVNCKIHPYIYIATNDSMSGFYAFTCSQMLNS
                     ESPNNCNSLYYKAE"
BASE COUNT         1399 a          728 c          901 g         1529 t
ORIGIN      
        1 cccaatgcct ttttcaaaat aaattaggat taaaataatt aaatcagtaa attccgatgc
       61 atgatttcgg atttttcgaa agtcctgatg aaaggctgcg ctacggcact cacggattta
      121 attgttattc ctaatgtatc tatgaattaa tgttttataa ggatttttta taaccattta
      181 taagtatttt caagcctggc ttgttgcaaa caatgtataa agcacttagg caataataat
      241 tacattcagc aactatcatc atcggtattg tttgtgggcg gaatttcata ttgacaacag
      301 tacagttctt atatctatta ataatagaaa gagatctaca acctacagat tggtgtagct
      361 ttatggaaaa agactatttg agaattagta gtactgtatt agtgagctta ttgtttgggc
      421 ttgctttggt tttggtgaat agttggttta atcagccagg cgttgaggaa gttgttccac
      481 gatcaaccta tctgatggta atgattgctt tattttttat cgatactgtt gcatttattt
      541 ttatgcagtt gtatttcatt tatgaccgta ggcaattttc aaactgtata cttagtttgg
      601 cttttctgag ttgtttgatt tactttgtta aaaccgtcat tatcattcag caaattattg
      661 aggggcgttt gacaagcagt gttgtccaaa atgacattgc aatctattat ttgtttcgtc
      721 agatgagttt gtgcatatta atatttctgg cattgatgaa taaagttagt gaaaacacaa
      781 aacagcgcaa tttattttcc aaaaaaatga ctttgtgcat aagtttgttt ttgtttttgg
      841 ggggccgata gttgctcaca tactttctag tcattatgag agctacaatt tacacattgc
      901 cgagttaacc aatgaaaatg gtcaggtggt ttggaaagcc tcatatgtta ccataatgat
      961 tttcatgtgg ttaaccttgc tttcggttaa tctatacttt aatggtttac gctatgatat
     1021 ctggaatgga gtgacggtga ttgctttttg tgccgtactg tacaatatct ctctgttatt
     1081 tatgagcagg tatagcgtgt ccacttggta tattagtcgc actattgaag tagtcagtaa
     1141 gttaaccgtt atggtaatat ttatgtgcca tatattcagt gcgctacgag taacaaagaa
     1201 cattgcacat cgcgatccct taaccaatat atttaacaga aattattttt ttaatgaact
     1261 gacagttcaa tcagcatcag ccaaaaaaac gccttattgt gtcatgatta tggatatcga
     1321 ccacttcaaa gaagtcaacg acacctgagg gcatccggtt ggcgatcagg tgataaaaac
     1381 agtggtgaat atcattggga aaagcatacg accagatgac cttttagcgc gcgtcggcgg
     1441 cgaagagttt ggcgtcttgc tgacggacat cgatactgaa cgtgcgaaag ctttagcgga
     1501 aaggattcgg gaaaatgttg agcgtttaac tggcgataat cctgaatacg ctataccaca
     1561 aaaagtgacg attagtattg gcgctgttgt gactcaggaa aatgcgttaa acccgaatga
     1621 gatctatcga ctggctgata acgcactata cgaggcgaaa gagaccgggc gtaataaggt
     1681 ggttgtgagg gatgtggtga gtttttgtga gtcaccataa agcggcattt tagcgggtga
     1741 ttttggtaag cgctaaatag ctattcgcag acttgcgtaa caattttcag aaatatcata
     1801 gtactatatg atcttaaatg cttatcattg atcaatctgc tatcacccgc ttttactggc
     1861 aattgtacta taatttctta ggttgcatca ccctgatgcg tggggaaaca tgtatggtat
     1921 tcaacaaaaa aatgtttgta ctcattataa taccaggcat tctgggggtg ttactatcgt
     1981 ttgctatgtc aatatttcaa atgaatagag atacgacaat taccgccggt attctgttaa
     2041 aacagcttga taatgttacg caaattgtcc agcatacgac aaagcttacc tctattctgg
     2101 tgatgaagcc ctgcaaagac attcttgaac agttgattgc taacggagca ttgactccct
     2161 atgttcgaac aacgggtctt atagaaaata atttccaaat atgttcttca gttagcggtt
     2221 tcaaaaaaat gaatgttaac gatgtttacg gtacgagctt tcacaataaa aataaagaat
     2281 cacgtatagt atcaattagc ggtacaagtt ttgtgccagg taaaacagca atagtgttcc
     2341 ttatgccaat agggaatgat atgacagctt ttagtattgt agagtcacga tatatctatg
     2401 atctaatgga tgttcttgat gatgagaatg atgattcatt ttctcttcgt tttactgaag
     2461 ggcctgcaat tattagcggt gtaaataata atgacaggtt atatatgtta aaaagagatt
     2521 ttaattctgc gatcagtcag gctcgtctta cagttactac gccgatgata tcgttatatc
     2581 cctatatcat tagcaatgtg ctttatattt tgccgttatc tattattctc tcttttattc
     2641 tctatttctt atggcagcac tggatgtcac ggaaaatgtc gctggcagaa gaaattaaaa
     2701 aaggaatgtc atccggagaa ttttctgtgc attatcaacc agtgtgcgac acgacaacca
     2761 aagcgtgtct gggggttgaa gcgttaatgc gctggcaacg cgaagatggt aaaaatatat
     2821 ctccggtagt ttttatccgc gctgcagaag aggaaaattt aataataccg ttaactaaac
     2881 atctctttga actgattatt caggatgtac aaagctggaa agtgaaaaag ccgtttcacc
     2941 ttgggataaa tattgccgcg gaacatcttg cacatcctga ctttgttggt gatgttttac
     3001 atattaaaaa tgcaatatct gataaattta atattgtcct tgagattacc gaacgcaatc
     3061 tggttgaaga cactgatctt gcattacaga aaataaatga attaaggatc catggctgtg
     3121 aatttgcagt cgatgatttt ggcacgggat attgttccct tggccttctg caaaagttgt
     3181 ctgttgatta tcttaaaata gataaaagct ttatagacac acttactaca gcggaagacg
     3241 aaacgccagt tttagatatt attattaagc tgagtaatcg gctcaatttg ataacgattg
     3301 cagaaggtgt cagtactcct caccagcaga atatcttatt cataataacg tgacattagt
     3361 tcagggatac ttatatgaaa aaccaatgaa agcgacagag ttttatcagt ggtatgtaaa
     3421 cagataaata attaaaaata ttggaatggt tttgtattgt cttaaaatat aaacaaagca
     3481 atcagaatgt tattaactga tgacaaacgc caagatcaac aatgctgtta gtgagtaata
     3541 catatgaatc gtggtgtcct gcctgtagag aatatgtagt tataatcatt ttgacgcgta
     3601 acataataat taattgaagt gtctgctttt atgaagaaat cagaagtgat attttcaagg
     3661 atttcatcgc tgatgttatc atctttttgt attttttgga ttttaactgt ggtgagataa
     3721 acggtatcac ggaatttctt gtatgtaaaa ctcacatatc ggcggatgaa gcccattttt
     3781 tttccatcta ccgtgtatgc gcccgtgaga tacatgtttc ctgtgttatt ttcagaaatc
     3841 atatttaacg aaaaatattg atccattttt gtatgatcgg tgaaattaat aacatgggca
     3901 ttacatgata tgttttgctc tgctggttca ttggaatgat gaaacgcaat atggctcaga
     3961 attgctgcaa taaaagcaca taaaattatt cccgaaaaca gaaaatatct ttgagtcata
     4021 tgattactcc gctttataat agagagagtt gcaattattt ggtgattcag agtttaacat
     4081 ttgactacag gtgaaagcat aaaacccgct catgctatca tttgttgcaa tgtaaatgta
     4141 aggatgtatt ttgcagttga cttccatttt ctgcaatttt tcaataattg gtgatagcat
     4201 gtctacggaa ttaaagtaat tattatcata gaaataactg catccttcct gagtagggtg
     4261 ttgagcataa tttgagaaag catctatttt ttttatagtg tgagatttgc tttgaaaagc
     4321 catatcgaaa aaataagaag acatataaac tatcccccat gccgcaaaaa aaaactataa
     4381 gtaatgtatt ctttgtggta aatatacctt ttgcaacgta atttttattt atattttcag
     4441 tgtgggtgcc attctcttca ctctcaacac tttcattctc attgtgatta ttgtcgagaa
     4501 cagttatgtt gatttcttca ttgattttga agcctttccg tggcaccgtt ctgataa
//
LOCUS       DAEEGA010000197         4551 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEEGA010000197 DAEEGA010000000
VERSION     DAEEGA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.199"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..1225
                     /locus_tag="KLH46_004093"
     CDS             <1..1225
                     /locus_tag="KLH46_004093"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBE0380549.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1225..1899
                     /locus_tag="KLH46_004094"
     CDS             1225..1899
                     /locus_tag="KLH46_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0380550.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2427..3188
                     /locus_tag="KLH46_004095"
     CDS             2427..3188
                     /locus_tag="KLH46_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBE0380551.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3232..4311
                     /locus_tag="KLH46_004096"
     CDS             3232..4311
                     /locus_tag="KLH46_004096"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE0380552.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4308..>4551
                     /locus_tag="KLH46_004097"
     CDS             4308..>4551
                     /locus_tag="KLH46_004097"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBE0380553.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1249 a          865 c         1119 g         1318 t
ORIGIN      
        1 ctttccgtat ggcattttcc agcagcgaaa tgttcttgcg agctggctgg caacaggttg
       61 cggcgtagcg ttgtatctgg ctttaacggc gcgtactcgt tctctgacgt tgatatgttt
      121 gttgtcgtta taccctctgt ctacagcttt agtccttaca caaagccgtg tcggccttct
      181 tggtttattt tgcgtcatcg tgctggccgc tctcgcggac agaccccgct ggcgcggacg
      241 tccccttagc ggtctgttgc gcttgatctt cgtcgcctcc ttgctgtgct ggtgtgtcgg
      301 tatctcaaac tatgccatgc caggggagac aacggctgac tttacgcatt catcatcgaa
      361 tgaccagcgt atacgtattc tgaaaggtgc cataagtctt attcaacagc gccccttaac
      421 cggttggggg ttaggcagct ttgaagcgac cttcccgcaa gccttagagg atgtgggcgt
      481 gacaaacata gagagtgata cggctacgca tccccataac gaactgctgt tcgtaacggc
      541 tgaaggtggc atcgtcgcct tgtttggttt tttgctgcta tcaggcgttt ggctgtggcc
      601 gtgtatatgc cgcctgaaaa tgacaaacaa taacgcgcac catcaatgtg aaaagtggtt
      661 tattgcctta atggggttgc ctttagtcat tcatatgatg accgaatatc ctctctatac
      721 gtctgttccc catttgatgc tgttgctgct ccttttccgc atcggaatgc cggaaaacgt
      781 gatgaatcag gtacgtatac ctaaaacagt ccggttgggt attctgcctg tcattgtgct
      841 cattatgacc gccgcgctgg tcatcctggt agcgggattt gatgcacagc gggaactgac
      901 cagagctgaa gctgatatga acgaaggatt tatccctcgc cttccggaac atagctggaa
      961 atacctgacg caggcagaac gtcttgagcg cgatcagcat ctgcttattg cgaataaacc
     1021 ggggttcgtt cgcgatacgg aaagcatgtc tcttttcact gtgtggggaa ataaatggtt
     1081 ggcaatccac aatgatgcag acgtaagcgc tgcaatgata ttcattgcgc acaggcgcgg
     1141 tgattatctg acagctgaaa aaataagaaa acgcgcagct cgggtatttg taaatgacga
     1201 acgttttact cgcgggggaa actaatggtt aacgatctga cgtataagaa attagaaagc
     1261 acaaacatca actgtaatat tctctcgcca gaaaatacca catggacaaa tgtcacggtg
     1321 attggcctgg atctttttgc catttgcggc gtgtgcagtt tgctcaagcg tgttggggga
     1381 gtgaaacttc aggttttcac ttccttaccc actgatatga atgttctgac tgacagtagc
     1441 atcgtgatat gggtaaaaat gcgtcacgat ggtttgcctg aattagccgg acatattgtg
     1501 aaggtttgtc gtcgataccg tctgatgcgg caactcgtta ttagcgatgc tattcctgaa
     1561 ggaatgacga atgatatcgg gcctctttcc cgagtatggc ttactcatgg gagtgcaaaa
     1621 gtggatattt tattatcttc attacggaag gtgttaaatg ctcattgttt gcctggtcct
     1681 ttgtttataa aacgcctggg aagagtgcaa tggagggttc ttttactccg cgcgaagggg
     1741 attagcacac gtaaaattgc agaaatctgt ggtattggcc tcaagaccgt tagcgcccat
     1801 gagtccgcaa ttcgagaacg attaggcacc aggaataaaa gtgagtacgt ctggttagta
     1861 cgtaatgctc aacttataca acaggcgata cccgcgtagg gaagagatgc gtcaaggtat
     1921 gaaagatgta acaatatgcg aacaaagcaa aaagtataat tgatgcgtca tagataactc
     1981 tctttttcga tgacgaaatc gctgagcatg cgtggtgagg ttagtaatag atgggtgcta
     2041 tgttttgcca cgggtttgta gctgtgaaac ctggaaaaaa ataagaatta aaaatattta
     2101 aagagagtta ataaggttct ggttgtaatt gtcttggtca aaaaaaagat attgtcaatg
     2161 ttgtttttta aattttcagg ttttggttaa atatttgctt acaaatatag gtgatttttt
     2221 tattgtaggc ttttgaaggc tattataact gtgcgctaaa ttaatgcgtt gaataaaaga
     2281 aataacaaaa ttaagaaaag ccatatttac gatatgggca acataaatat aatctaaaaa
     2341 agagtaaggc gttgtaggtt ggttccgcgt tcattacctt gttagtgaac gcggatttat
     2401 cagttgtcga aatggttttt ataataatga aaaattttaa tgtactaacc aaagtcgtat
     2461 tttgcttgtc gtttttatca agcgcatttc ccacctatgc tgcggatgat gtcaaagtga
     2521 ctcaaaacac ttcagttttt aaactgaagc ttagtgcaac gcgattaatc tatgaacaag
     2581 ggtcggatgg cgcagcgata actgtgacaa atccgcagga ttggccgatg cttgtgcaga
     2641 gtaaagtctt tgaagaagat aaagtaactg cagcgccctt tattgtcact ccgccactgt
     2701 tcaggcttaa tccaggccag caaaataaaa tgtgggttgt gcagactgcg ccagctttac
     2761 aagacaggga aaaattacac tggctatgcg ccaccggcat tacgcctaaa aagagtgatg
     2821 tttgggccga gaaagataaa gaaaaaattg ctgaaaatgt gaacctgaac atccaggtta
     2881 acgtacatac ctgcataaaa cttattacca ggccatctgg tattgaaggc aagatggtta
     2941 atgcgagtga gaagttatcg tggaaaaagc agggtgataa aattgtcgcg gtcaatcagt
     3001 cgccgttcta catcaacctt tcttctttac aggttggcgg tagcaagatt gagaaactag
     3061 attatattgc gccgttttcg caacatgagt ttgttatgcc aaaaactagc ggccaggata
     3121 ttaaatggtc tgtgattact gatgaaggtg gtgagagtcg tttgtttata tctcatttgc
     3181 aatcataatg tattacattt ttattgaata tttttatgcc atgcatattc catgttttat
     3241 cgactgtcat taattttgat aatggcctta cttgcgggtc agttaagtgc ccaggagtgg
     3301 tcatttgatt cttcacagtt ggaaggaaac gttagtgcgg acactgtggc aatgtttaat
     3361 cagggagagc aactccccgg aaattatcgt gtggaaattt atcttaacgg tgaaaaagtt
     3421 gatgttgggg agtttccctt tcatcgtccg gaatcccctg aagaaaaaga gcttgttcca
     3481 tgtttaaccg ttgatgacct tatccactat ggcattaaaa ttgataaaag cagcagtgat
     3541 actgacaata aaaagaatca gtgttttaaa tggaactcta ttgaaggttt gaaagtaaat
     3601 tatgattttg atagccagcg cgtacagata accgttccac aactgtatct gcaagataaa
     3661 aaaagtagtt tagcaccagt gtcgttatgg aatgagggtg tagctgcatt ccgcatggtg
     3721 tatcagacaa atattgatat ttccaaacaa aatgataatc aatctaccac gcgtaattct
     3781 cgttatggca ggttcactcc agggtttaat cttggtgcct ggaggtttcg tagttcagtg
     3841 acatggagca aggaacttgg ccagtcagaa agatggcaaa ggggttacat gtggtttgag
     3901 cgaggcataa atgcgattaa aagccgatta acgcttggag aatcttatac gtcgtctgag
     3961 gtgtttgaca gcataccttt tcgcggcggg atgttagcga ccgacgatgc gatgactccg
     4021 ccggaggaca gctactacac acctgtggtg catggaatag cacaaagtga ggcgcaggtt
     4081 attattaaac aaaatgggca aattattttt acccgttctg tgccgccagg gccttttgcg
     4141 ctggataatt taccaacgct ggccgttggc ggggagcttg atgttacagt gagagagagt
     4201 aatggcgagg agcagtattt ttccgtgcca tttcaaacgc cggccatcgc tctgcatgaa
     4261 ggatatttca aatatagtgt gatggggggg aatataaaga agaaggtata aaaagctccc
     4321 ctgtctccgt gggcgaaatc acggcgatgt acgggcttcc ttatggcgtc acggtgtatg
     4381 gagggattca aaacgccact cattttaatg caatatctac gggtattggc atttctctgg
     4441 ggctgttagg gagtttatcg acagatatta cgcgttcgat tgcgaattta tattatggga
     4501 ataaatatcg tattcgttat agtaaatcca ttagtgattt tggtactcaa t
//
LOCUS       DAEEGA010000198         4464 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEEGA010000198 DAEEGA010000000
VERSION     DAEEGA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.200"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(139..1041)
                     /locus_tag="KLH46_004098"
                     /pseudo
     CDS             complement(139..1041)
                     /locus_tag="KLH46_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1101..1379
                     /locus_tag="KLH46_004099"
     CDS             1101..1379
                     /locus_tag="KLH46_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380554.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="KLH46_004100"
     CDS             complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="KLH46_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBE0380555.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="KLH46_004101"
     CDS             complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="KLH46_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBE0380556.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3533..3865
                     /gene="hdeA"
                     /locus_tag="KLH46_004102"
     CDS             3533..3865
                     /gene="hdeA"
                     /locus_tag="KLH46_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBE0380557.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3981..4307
                     /gene="hdeB"
                     /locus_tag="KLH46_004103"
     CDS             3981..4307
                     /gene="hdeB"
                     /locus_tag="KLH46_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBE0380558.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4371..>4464
                     /locus_tag="KLH46_004104"
     CDS             4371..>4464
                     /locus_tag="KLH46_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="inner membrane protein involved in cell
                     density-dependent acid resistance; part of the acid
                     fitness island (AFI) of E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBE0380559.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAG"
BASE COUNT         1312 a          954 c          943 g         1255 t
ORIGIN      
        1 tttatcgata tcgaagcatt gagcattttt aactcgtttt atcttgaatg caatcggcgc
       61 ataacttatt gaatcccatc aaatcccgtc tttttttacc cattcccggt tatcttgaat
      121 aaattaccta gtcataaata cattctggcg gctaccttca tccactaatg ccgtgacata
      181 catgccaggc agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc
      241 cgtggtttca tcaaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtaacgttt
      301 accattttcc agattgagct gtactggcgt actgccctga acctgtttga tttgcccact
      361 ggcgacctct tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat
      421 cgggtccaga cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc
      481 gacggtcacc gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg
      541 cagattgatc gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc
      601 attcagctgg gtgcgcgcgg tgtcgtaatc ctgacggcta acgtagttgg tcttcagcaa
      661 cgatgcctgg cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag
      721 cgagcctttg gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga
      781 atcgccctgg ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac
      841 ctggggacgt atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac
      901 attgaccgaa ccaggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggcgtt
      961 ttccgccgat ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa
     1021 cagctttctt cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta
     1081 aattgcgcgc gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag
     1141 ccagtaaggt gggatacagg cacagtgatc gacatggtga ggtcaacgac aaaaaaaatg
     1201 ttgccgttct gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat
     1261 acacatacca ggggaatcaa gtcgttttag cctctccttt agtaatcacc gggggtaatg
     1321 aaatcagcat ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtcctgac
     1381 taaaaataag atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagcttg
     1441 tacataatgt tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg
     1501 atattgcttt catttttatt tttcagaatc agttgcataa ttttgaactg attatgtgaa
     1561 aaacataatt gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt
     1621 ttgatacttt ctttgcggct aacaaaataa ataggcgctt tagcttttag tttactgatg
     1681 tgataaggtg ccaaaacaat aattctctcg gcatctaatt tctccagaaa tttaatcgct
     1741 tcttcatcaa ggatatgatt gtgataggta tcaatgataa ccttgaaggg tttattgcct
     1801 acttttttga tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc
     1861 caaaagccct gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgctcctta
     1921 gccgttatcg tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg
     1981 ttcaatatag taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca
     2041 ctaatcaata cttcagattt atccatcacg cactccatat attgatccta agtaagattc
     2101 ctggttgtta tcagcttgta aatagtaaac aaaaagttaa cgtttgcgga ttactgccca
     2161 agaataagtt atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaatgt
     2221 aatccgattt aaatatcgag tctccttgtt tcgacttaag ctggcaattg gattgccagc
     2281 tttcttttaa cgcattgaaa ttacctgagt aaataacgca ttcatgtctt caggttttta
     2341 tatagcgtct ggagacacgg atatttatgc aatgtaatca acgccagttg caaaaaaatg
     2401 aacccggatt acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact
     2461 ttcccacagc acttttaaaa cattaaacct gactacggaa aatatcagcc atgataatgt
     2521 ttgaaatggc taatttgcca tggagtgaaa aaaattagat gaaattcagt aggttgaaat
     2581 aatcactagc aggtaattat ttcaatgata gtgcgcaatt gatctacaac actgcgtagc
     2641 ggagagagta ttaatcggat catagtcaca tcaagtgact atgatccggg tgacaaccgg
     2701 ggtaattatt gctgcttaac gaacaaactg gcgaagctga acaggctggc ggcgctaaat
     2761 atcagttcaa ttcccaccag tgtggaaacc agcgttacag acaccatcgg cgttgcacca
     2821 aggaatatcc aggcaatgac gatatccagc acaccaataa cgagctgtag ccagctgcct
     2881 ttcattgaac gctgacgata ccaactcatc aggcgaataa cccctgcaac acagaacaaa
     2941 ccggcaataa atgccgcaat ggcaaaaatg cccagctccg gtgcacggat gaagaaatag
     3001 ccgatcaata aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga
     3061 ctgcggttgc tgaataaccc gacaataagc gcaatacccg agcagattaa taatgcaccc
     3121 actactgtgc ttaaaatatc gccagagacg aacgggaaac tgatacacag caacccgacg
     3181 ataaacagca gcacggcaat aaactggatt gctctgcgat gctttttcag catctccaga
     3241 tcaaacttca aaattgttgc cttatctata tataacatag aaccacccta taaaattaag
     3301 aagaaaatcc cctgctatca atctatgcca aaaacgcgtc taagaatgca gtcgatttaa
     3361 taaaaatttc ctaattgcag tatctgatgc atctgtaact cattgtattg aaataaaaat
     3421 atctgatttt gatattttcc atcaacatga catatacaga aaaccaggtt ataacctcag
     3481 tgtcgaaatt gattcgtgac ggctctttca ctttatagtt gaggatatta cgatgaaaaa
     3541 agtattaggc gttattcttg gtggtctgct tcttctgcca gttgtgagca atgcagcgga
     3601 tgcgcaaaaa gcagctgata acaaaaaacc ggtcaactca tggacctgtg aagatttcct
     3661 ggctgtggac gaatccttcc agccaactgc agttggtttt gctgaagcgc tgaacaacaa
     3721 agataaacca gaagatgcgg ttttagatgt tcagggtatt gcaaccgtaa ccccagctat
     3781 cgttcaggct tgtactcagg ataaacaagc caactttaaa gataaagtta aaggcgaatg
     3841 ggacaaaatt aagaaagata tgtaattccg ggaatgcgtt acatcgtact tccttgcata
     3901 ttgaacaggc cggaatatct tctttaaaag cagctattcc tcctgttcat atataatctc
     3961 tatattgaat gggttacaaa atgaatattt catctctccg taaagcgttt atttttatgg
     4021 gcgctgtagc ggctttgtca ctggtgaacg cacaatctgc gctggcagcc aatgaatctg
     4081 ctaaagatat gacctgccag gaatttattg atctgaatcc aaaagcaatg accccggttg
     4141 catggtggat gctgcatgaa gaaacagtat ataaaggtgg cgataccgtt actttaaatg
     4201 aaaccgatct cactcaaatt cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa
     4261 atttgtatac cttcaaaaat caagcatcta atgacttgcc gaattaatga ggtacaagta
     4321 aaaaggagta gcaagttgag ccatcttgct gctccttttt gcatttttat atgacagcag
     4381 aatttattat acgtcttata ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc
     4441 aaatgcgcgg caaaggagca gggt
//
LOCUS       DAEEGA010000199         4323 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEEGA010000199 DAEEGA010000000
VERSION     DAEEGA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.201"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..157)
                     /locus_tag="KLH46_004105"
     CDS             complement(<1..157)
                     /locus_tag="KLH46_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBE0380560.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTY"
     gene            complement(307..468)
                     /locus_tag="KLH46_004106"
     CDS             complement(307..468)
                     /locus_tag="KLH46_004106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380561.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(465..653)
                     /locus_tag="KLH46_004107"
     CDS             complement(465..653)
                     /locus_tag="KLH46_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBE0380562.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            913..1248
                     /gene="iraM"
                     /locus_tag="KLH46_004108"
     CDS             913..1248
                     /gene="iraM"
                     /locus_tag="KLH46_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBE0380563.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1529..1637)
                     /locus_tag="KLH46_004109"
                     /pseudo
     CDS             complement(1529..1637)
                     /locus_tag="KLH46_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2160..2236)
                     /locus_tag="KLH46_004110"
     tRNA            complement(2160..2236)
                     /locus_tag="KLH46_004110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2200..2202),aa:Arg,seq:tct)
     gene            complement(2338..2413)
                     /locus_tag="KLH46_004111"
     tRNA            complement(2338..2413)
                     /locus_tag="KLH46_004111"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2378..2380),aa:Ile,seq:cat)
     gene            complement(2558..3379)
                     /locus_tag="KLH46_004112"
     CDS             complement(2558..3379)
                     /locus_tag="KLH46_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBE0380564.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3394..3750)
                     /locus_tag="KLH46_004113"
     CDS             complement(3394..3750)
                     /locus_tag="KLH46_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBE0380565.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3763..>4323)
                     /locus_tag="KLH46_004114"
     CDS             complement(3763..>4323)
                     /locus_tag="KLH46_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBE0380566.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNE
                     KYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLA
                     FEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1187 a         1178 c          856 g         1102 t
ORIGIN      
        1 gatatgtcag cagcccaaac agcagactcc ccagcacgcc tattgccgcc cactgagacg
       61 gggaaaccct gtccagcaac tgcaggaacc agtagcccgt tcccaccgct gacgtggtgt
      121 atgacacacc tgttgtgatt ttttccatct ggtccatacc ccgtctcccg ttatccggaa
      181 gctgacaaca ataaaaaagc caccagttaa ctactgatgg ctctgataac tcatgcaagc
      241 gtctcagacg acccactgac actaccggtg agtttaacga taccttccat ttgactggct
      301 cactttttat gatgatgccg gtgcatttat ctccagcacc agactttcta tctcaacgcc
      361 atacgttgca tttttggtaa tatccgtcag cgtcagtgca tttagtccca ctgccagact
      421 gtcttttatg gcctggaatg ccgggccagt acgatgacgt agtatcactc cggctcagtt
      481 gcaccactga ccaccacatc accttctgct gcaatcgcct gcatcagggt ataaggggtt
      541 atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt cagcttttcc
      601 gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc catggttctt
      661 tccaacatct gaaaaataat tctttaaaat acctgacatg taatacagaa aaaacacaaa
      721 accatacctt aaataaaaac ctgattatca agcagatatg catggataaa ctacaagacg
      781 agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa taattctgct
      841 ctatggttta attcaaaaat atcatttata cttttcagaa catcaccagc aaggcataaa
      901 caaggaaact aaatgaagtg gattgtgatt gatacagtta tccagccatc atgcggaata
      961 tctttttcag tcatatggag taaaataaaa ttaataatct ggtatcaatc ggatgctttc
     1021 ttacctcctg aaagtatatt tacactgact cacacaggca tcatgctcaa taacaaagtg
     1081 ctacctgtaa ccatttacaa cgtagtacca ttcaataaaa cattctggaa tttaatcaaa
     1141 aacagccagg aatgccctac aaatacagat aacgtattga atgaatgctt taataaccgt
     1201 tgcactctgc aaatatgtcc ttatggacta aaacaacaaa gtccataagg agtttactca
     1261 catctgacaa aatcaatata aacagcccct ccggagaggg gctggagagt ggcgctatgt
     1321 gccattccat ggtgccgggt gcctcccggt gaattcagta ccagcacctg aatccgcgat
     1381 tatcccatat acctactcgc tgattgcccc tccgcacagg gggattcacc atgccagttt
     1441 cttttaacaa actccccgca aaccagacaa cagtcaaccg cctgaattgt gaagtattta
     1501 aaaatttctc ccgctaactg atacccggct aacagtctgg cgttttcttt ttcagcaacg
     1561 ggaaagcaac aaccaccaca cccgccacca gcacaccgtc agccggcact gacattatcc
     1621 ggctgctgca atgccattaa caaaaacagt aagcaatcac tttttaccgt aacaggtgat
     1681 aatccagata tgtatctacc ccagatgagt aatccgaagt tcatccatac cacaggtcct
     1741 ggctattctg ttgtactcct gaacaagagc aaataattct gaattagcaa ccatgaactc
     1801 atcgcaaacc ctctgtatag catcactatt cagaataata acgtctcttc ccgaaagacg
     1861 atcaggagta cagaacaaaa ctgtcaaacg gctgaaggcc tttgctcgtg ctgcattgac
     1921 tatatcaata cgctgcctaa ggatgaaaca ccccgacgcc tcatcaatat tcactctacc
     1981 cacaccatat gaatgataaa tacttaatgc tgaaaaaacc attagaccgt ataacaaata
     2041 ctcaatcaat acttaacaga acttttattt ttgacaaata tataatattt tcaacaatat
     2101 cctgagccag gtatatttca gtataaggct ctgccggaag gaatctggaa gaatgaatat
     2161 ggcgcgctgt actggattcg aaccagtgac cgattgctta gaaggcaatt gctctgtccg
     2221 gctgagctaa caacgctgaa taccgataat ggaccgccat cggggacccg ccccccgcac
     2281 caacaaccct gttatcgtgt cgtctgctct tcctgataag ctaatggcgg tttgtgatgg
     2341 tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc tctcaccact
     2401 gagctaaagg gccgggagca gaataataac ggtccgtaat taatttcgca ataaaaaaac
     2461 ccgctcagcg gcgggttgta gaaactcttc taacgccagg cataaaacgc ccatcattat
     2521 gacgaattta ccacagattc cggaaaaatc aaccttgtta cctagttacc ttttttaact
     2581 gccgctcagc ccatgcttct tcaatatcaa accgggtcac cagcgcatca taaaatttct
     2641 taactgtttt ttcccatgac gcgcgtgtta tctggtttgt cacctcgcat atagcattaa
     2701 atgcctccgt tgatggtagt ctttcatagc cacgaccacc acaacgctgg cagtctctga
     2761 taacaggcat accacgtttt accgactctt cacgatgaat ggcgacacca cgcccacggc
     2821 aatccttaca ggcggtggaa acctcaccct ttccgccaca ctccggacag gcaactttta
     2881 ccacctccct gacttttttc cattcttccc agtaagacgg atacacacct ttcgtacact
     2941 ttgcccatac cggcggctta ccatccggat actggatctt gtttgtaaaa actatgcttt
     3001 caataaattt ttccccatag caacaagggc actgcttttt actcgctgcg ctgcgggcat
     3061 aatcctcaaa agcgtacgaa gccataatgc gcatcactac cggttttatt tctgccggaa
     3121 gttttctcaa cgccgccaca cgatcgcacc gactgagtgc ataatctgcc agtaattctg
     3181 ttgcccgcgc cctgtcattc atactgatgc ccattttccc caggaacgca gaaaaaccca
     3241 tctcagcccg attctgtgtc atgccctgcg cggccatcac atcagtgata ctcagcgcat
     3301 cttttgacgt tgaggccgat gcatcggtca ggccagggga ttttggggag tagtatttcg
     3361 gtaaatcttc cagtttcatt ttttgacctg ctcttaatgc attatggggt aaatcttcac
     3421 ccccagacgt ccaccagata ctggctgacc acgaacgata ttgatttcat caaactgctc
     3481 atcgtccatt aacactcccg catgcgtcag cgcatccagc ggtgctttca ggatattgtc
     3541 caggtcgcga cgacgcttat ccggtggctc tgcaatcacc tttatcgcca gccttccgga
     3601 caggcttaat ttcagccgct gctggcgaac aataagcgcc acagcccggc gataacgctt
     3661 tccctcctcc gagataaaat atgtgctgcc acggcgtcgc cagtaagtgt tcaccgtcgg
     3721 cgggtaaggt aaaaccaaat ctatgagcat cagtcacctc ttttacccaa gcacgccagt
     3781 tgcaaaggcg tgatcaagaa aacgaaaaat taaatcaacc tgagaaccat gcttttcttc
     3841 gaacgccaga ggatccgcat gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa
     3901 aatatcgtga gatttggtcc ccattccgcc ctgaccatga ccaatcaggt gatggggatc
     3961 gtcggctggc ttaccacaac acgcacacgg ctgtgtcttc acccagcgtt tgtatttctc
     4021 gttaacccag cggcgacgtt taggtcgttt catgaaagat tccggagact caggatcaac
     4081 ggcaatgctg accaccgtct tttcctgttg tgggttctgt tgctggtggg cgtgaggcag
     4141 cggcgcaaga ttttttgtgc gctgtttcag tatgctggtg gcggtctgct ctcccggtac
     4201 gatgtcgctt tcacggtaca ttgagcggat tttttccgca cgcaacccca gcgaacgacg
     4261 taataccgct tccggtagcg cgtccgccac ctgattgcgg accgcccacc aggataattc
     4321 agc
//
LOCUS       DAEEGA010000200         4308 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEEGA010000200 DAEEGA010000000
VERSION     DAEEGA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.202"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..335)
                     /gene="flhe"
                     /locus_tag="KLH46_004115"
     CDS             complement(<1..335)
                     /gene="flhe"
                     /locus_tag="KLH46_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HBE0380567.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGG"
     gene            complement(335..2412)
                     /gene="flhA"
                     /locus_tag="KLH46_004116"
                     /pseudo
     CDS             complement(335..2412)
                     /gene="flhA"
                     /locus_tag="KLH46_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2405..3552)
                     /gene="flhB"
                     /locus_tag="KLH46_004117"
                     /pseudo
     CDS             complement(2405..3552)
                     /gene="flhB"
                     /locus_tag="KLH46_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3788..4171
                     /locus_tag="KLH46_004118"
                     /pseudo
     CDS             3788..4171
                     /locus_tag="KLH46_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(4181..>4308)
                     /locus_tag="KLH46_004119"
     CDS             complement(4181..>4308)
                     /locus_tag="KLH46_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0380568.1"
                     /translation="ITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC"
BASE COUNT         1070 a         1238 c         1056 g          944 t
ORIGIN      
        1 ccaccgcctg gcacttccca gataaatcgc aacggttctg ctgccgctat accggaaaag
       61 gccacggtgg taccgctctg cccctctaat tcgacacaac gagattgcga acacaagcga
      121 acccgcagtc ctgcaggtgt cgggccgata agctgataac gccacgccac cagcgtcatc
      181 aatcctgaag caggttgtcg cgcagaaaga ggtgcagacg acatcgactc accgcgatga
      241 tttagcgtaa ttccgacact acttgcctgc cacatcccct ccccggcggc ttgcaccagc
      301 agcggaaaca ataatatcgc taataaggtt ctcatcattt accgccaatt gtcgccgtca
      361 tgcggatatg tcggttatca gacagttcca gattcgacag gaccactaac tgcggcaagc
      421 tgcggcgcag gaagcgagac aataatggtc gcagcgcgtg gttcaccaac aatactggcg
      481 gcgcacccag catctcctga cgggatagag cttcctgagt ttgcgccagt aaacgatccg
      541 ccagccctgg ctccagtcct cccccgccct gcagcgcctg tagtaacaaa cgttccagcg
      601 gtgtatcgag gccaataaca tggacttcat ctttgccagg aaaccactgc tgggtaatcg
      661 cccgtcccaa cgccacgcgc acgacggcgg ttaattcatg tggatcgctt tggatgggcg
      721 catgttccgc cagcgtttcg agaatggtgc gcatatcgcg aatcggtact ttttcatcga
      781 ggagattttg cagcacttta tgcagtgtgg tgagcgtgac gacgccagga acgagatctt
      841 ccgtcagctt tggcatctcc tgggcgacgc gatccaacag ctgttgcgcc tcctgacgac
      901 caaacagctc tgcggcatgc tggctaatga ggtggttaag atgcgttgct accaccgtgc
      961 tggcctcaac cactgtgtac ccctgaatct gcgcctgttc ttttagcgca ctttcaatcc
     1021 agatagcatt caggccaaat gccggatcgg cggtcgcctc accaggtaac gtcccggcag
     1081 cggttccagg gttaatcgcc agccagcgcc ccggataagc atcaccactg ccaatctcca
     1141 cgcctttcat caaaatgcga tagcgggcag gttgcagatc catattgtcg cgaatgtgca
     1201 ccactggcgg cagaaatccc atctcctggg caaatttctt gcggatactg cgtatacggc
     1261 ccaacaactc accatcctgc tggaaatcga ccatcgggat cagtcgataa cccacttcca
     1321 ttcccagaga atcttccagt tgtacatcgt tccacgtcgc ttcgacaacg gtattattct
     1381 ctgccatttt taccggtttg ggttcggcag gcgctttttg ttcgcgtccg cgtatccacc
     1441 aggccagccc cagcaatccg gcagtgaaca gcaaaaatac caggttcggc attccaggca
     1501 ccaggccgag taaaccgagc acggcggcgc ttaacaacat aacgcttggg ttactgaaaa
     1561 gctgattcac catctgctcg ccaacatcct gatcggtgct gacacgcgta acgatgcccc
     1621 ccgcggcggt agaaatcacc agcgccggaa tttgtgccac cagaccgtcg ccaatggtca
     1681 atagcgtata actttccgcc gcgtgtccca tgctcatgcc agttgcagca cgccgaccag
     1741 caacccgccg acaacgttaa tgaccatgat gaggatcccg gcaatggcat cgccgcgaac
     1801 aaacttactt gccccgtcca ttgagccgta aaaatcggct tcctgagtca cttcggagcg
     1861 gcgttttttc gcctcatctt caccaatcaa tccagcgtta aggtcggcgt caatcgccat
     1921 ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc acttctgcta tacgcccggc
     1981 acctttggta atgaccataa agttgatgat cacgagaatg acaaacacca cgataccgat
     2041 agcgaaattg ccaccaacga ggaagtgacc gaacgcttcg accaccttcc cagccgccgc
     2101 cgcgccggta tgcccttcca tcaaaatgat acgggttgaa gccacgttaa gtgccagacg
     2161 caacaacgta gtaaacaaca gaatggtcgg aaacgcagca aactcaagcg tgcgctgggt
     2221 aaacatcgcc accagcaaca ccatgatcga caaggcaata ttgaaggtaa acaacaggtc
     2281 gagtatgaat gcgggcagtg gcagcaccat catcgacaag atcaacagga tcaaaatcgg
     2341 tccggcaagg atctgccatt atgtcgattt caggtttgcg ggcaggcgca gcatcgcggc
     2401 cagattactc atgggtcggt ttctcgttaa taaaatccag ggctgccggc accggaagat
     2461 gagtaggtgt acagggcgct gtccaccagc cagacgccag cgtttcagtt gccagaccca
     2521 ggccagcact tccgccaccg cggcgtacag ttgacccggg atttgttgac caatctccgc
     2581 atgtcgatac agcgctcgcg ccagcggcgg cgcttcaagc gtcgggacgt tattttcagc
     2641 gccaatttca cgaatgcgca gcgcgaccag ccctgcacct ttagcgacca ctttcggtgc
     2701 gctcattttg ttttcgtcat actgcaacgc taccgaatag tgggtcgggt tattgacaat
     2761 gacatccgct ttcggcacat cggccatcat ccgacgccgt gcggcagctc gctgcatctg
     2821 acagatccgt cctttaacat gggggtcgcc ttcgccttgt ttgaactcat cacgaatatc
     2881 ctgccgcgac attcgcagct ttttcaggtg gctgaagatt tggaaaaaga cgtcaaatcc
     2941 caccattgga atgacaccaa gcaccaccag cagtgcgcat agccctacca aatccatcgc
     3001 attacccatg gcggtaatcg gtgactcggc cattaagcgc atcatctgcg gccagtgatg
     3061 ccagagaaaa aaccccgtca cgctgccaac caggatggtt ttcaaaattg ccttaagcaa
     3121 ctccgcgcta gtctgtgccg agaatatccg tttaatgccc ggtagcgggt tgagtttgga
     3181 aaacttcggc tgcaaggatt tgccgctaaa taccagcccc cccagcataa ccggagaaat
     3241 gagcgccacc agcaccacgc cgctaatcag cggtagcagc gccagcatgg cttctctgat
     3301 cagcagaata atttgcccga ggatcagatt cgggtctttg ataatactgt gatcaaaatg
     3361 cagcccagcg gagagcatgc ccgacaatcg acgggccagc gacacaccgc caaaccagat
     3421 aacactaacg cccaccagca aaatcagcag tgaggtcagt tcacgggaac gcgggatttg
     3481 cccctcttcc cgcgcttttt ctagtcggtg aggtgtgggg gcttctgttt tgtcgtcgct
     3541 ctcgtcagac acgtcgccaa tcctggaaag agtattaagc gtaaatgatg ccagagcgca
     3601 aagcgttcaa tggtttaagt aaggggcaaa acaggcggga tttagggcgt ttatgcaaaa
     3661 ataggttcag ttgcagaacg ttttgcgaac cattttccag ttctgatatt tagccattta
     3721 gcctatagaa tcgcactccg cctctctgtt agtttgctaa taattaaatt attacaaagg
     3781 tgatcaaatg gaatggcaac aattgcttaa ctgcaaccgt cggaaagata atctaaaaaa
     3841 aaccaacata gcatttttac atccccttgc tagcggaaga caagagatcg agcgggatta
     3901 cgaccgatta ctttttgctg caccaactcg tcggcttgcg gataaaacac aagttttccc
     3961 gttagatcaa aatgacagca tacggacgag gctgactcat tctcacgaag tagcaaactt
     4021 tgctcgcggg ataggaatga ggctcgcttt cgagatgaag gagcacatct ttggcgaaat
     4081 acccacgggg attgtcgttg aacgcgacgt tcctgctctg ttagcagcga tcggactggc
     4141 acacgatctc ggtaatccac catttgggca tctgaagtgg tcaacaaaaa ctggccaccg
     4201 agttagagtt tttccagtat cgattttccg attcgtttgg gggtaaccca ccgttatatt
     4261 cgtgcggtct tagtgcactg taatatccaa cgatatagtc cgttatgg
//
LOCUS       DAEEGA010000201         4246 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEEGA010000201 DAEEGA010000000
VERSION     DAEEGA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.203"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            33..914
                     /gene="yddG"
                     /locus_tag="KLH46_004120"
     CDS             33..914
                     /gene="yddG"
                     /locus_tag="KLH46_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBE0380569.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1169..2269
                     /gene="ompC"
                     /locus_tag="KLH46_004121"
     CDS             1169..2269
                     /gene="ompC"
                     /locus_tag="KLH46_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBE0380570.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            2433..2918
                     /locus_tag="KLH46_004122"
     CDS             2433..2918
                     /locus_tag="KLH46_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBE0380571.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            3028..4017
                     /locus_tag="KLH46_004123"
     CDS             3028..4017
                     /locus_tag="KLH46_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBE0380572.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         1316 a          875 c          805 g         1250 t
ORIGIN      
        1 tttttaacgg caagagagac aaaacagcga gcatgacacg acaaaaagca acgctcatag
       61 ggctaatagc gatcgtcctg tggagcacga tggtaggatt gattcgcggt gtcagcgagg
      121 ggctcggccc ggtcggcggc gcagctgcta tctattcatt aagcgggctg ctgttaatct
      181 tcacggttgg atttccgcgt attcggcaga ttccgaaagg ctatttactc gccgggagtc
      241 tgttattcgt cagctatgaa atctgtctgg cgctttcctt agggtatgcg gcgacccgtc
      301 atcaggcgat tgaagtgggt atggtgaact atctgtggcc cagcctgaca attctctttg
      361 ccattctgtt taatggtcag aaaaccaact ggctgattgt accaggatta ttattagccc
      421 tggtcggcgt ctgctgggtg ttaggcggtg agaatgggtt acattatgat gaaatcatca
      481 ataatatcac caccagccca ttgagttatt tcctggcgtt tattggtgcg tttatctggg
      541 cagcctattg cacagtaacg aataaatacg cacgcggatt taacggaatt accgtttttg
      601 tcctgctaac gggagcatgt ctgtggattt actattttct cacgccacaa ccagaaatgg
      661 tatttagcac gcccgtcatg attaaactca tctctgcggc atttacctta ggatttgctt
      721 atactgcatg gaatgtcggt atattgcatg gcaatgtcac cataatggcg gtaggctcgt
      781 attttacgcc tgtactttcc tcagcgcttg cagccgtgct gctcagcgcc ccgctgtcgt
      841 tctcgttctg gcaaggcgca ctaatggtct gcggcggttc cctgctctgc tggctggcga
      901 cacgtcgtgg ttaaaataat tgccgggtct acggcccggc agttaatcag ataaattccg
      961 acaatattaa aaaataaatt tcaaatacca gtaattcacc ttatatctca tttcgacttc
     1021 gatcacatat cgataaataa tattattcta tttttctcat tgtttttaat caccattcta
     1081 ttttaaaaac agtatgtcat ttataaatga cgccgtttaa aattcgttta gaaaatacgt
     1141 ttaacgataa ttaaaaggat tctgttaaat gaaattaaaa atagttgcgg tggttgtaac
     1201 tggtttgtta gctgcgaacg tagcacacgc tgccgaagtc tataacaagg atggtaataa
     1261 actcgacctt tatggcaagg ttaccgctct acgttatttt actgatgata agcgtgacga
     1321 tggtgataaa acttatgccc gtctcggctt taaaggagaa acgcaaatca atgatcaaat
     1381 gattggtttt ggtcactggg aatatgattt taaaggctat aacgatgaag ccaacggctc
     1441 gcgcggcaac aagacccgtc tggcctatgc tggtttaaaa attagtgaat ttggctctct
     1501 ggactatggt cgtaactacg gtgtcggcta cgacattggt tcatggaccg atatgttgcc
     1561 agaatttggt ggcgatacct ggagtcagaa agatgtcttc atgacatacc gtaccaccgg
     1621 tctagcaacc tatcgcaact acgatttctt tggcttaatt gaagggctga actttgccgc
     1681 gcaatatcaa ggcaaaaatg aacgtactga caacagtcat ctttatggtg ctgactacac
     1741 gcgtgccaac ggtgacggtt tcggtatctc ctcaacttat gtttatgatg gctttggtat
     1801 cggtgcggta tataccaaat ccgatcggac aaatgcgcag gaaagagccg cagctaatcc
     1861 tctcaatgcc tccggtaaga atgcagaact atgggccaca ggtataaagt atgatgccaa
     1921 caacatctac tttgcagcta attacgatga aacattaaac atgaccacct atggggatgg
     1981 ttatatctct aacaaagcac aaagttttga agtggtggcg caatatcaat tcgacttcgg
     2041 cttgcgccca tcactcgctt acctgaaatc gaaaggcaga gatctgggcc gctacgccga
     2101 ccaggacatg attgagtata tcgacgttgg tgcgacgtat ttcttcaaca aaaatatgtc
     2161 gacctatgtt gattataaaa tcaacctgat tgatgaaagc gactttaccc gtgccgtaga
     2221 tattcgcacc gataacatcg tcgctacggg cattacctat cagttctaac gtcctctcct
     2281 gaccagccct tctctgggct ggtttttgat ttaagtgctg ctgtatgact ccttaagtaa
     2341 aaatgagcaa ttcttgatat ttctggtcat tcacctcaat taatattatt ccatatcctc
     2401 cggggacatc ttactataat atttattgat gagtgattat gaccaatatt aatacagctt
     2461 gtgtaaaaaa taatgccagt tatcaactta ataacgcatt acctaacaaa gaaaccatct
     2521 ccagcaattt ttgtgaacga ctagaacaat ggggtaataa atcggttaat aatggtgaag
     2581 aaagagcaat tgccgtggag cgaattaaag aagcttacaa ttcgaacatg gcatctcttg
     2641 atttgtcata tcttgatttg agtgaattac cgcctatccc ctcgacagtt aatacgttaa
     2701 atctggaaaa caattgtcta acttgtcttg acttagctga caatgccagt ctcgttaata
     2761 tcaacctcag cgttaataaa attaacacga taacctttcc aaaagaacca aaactggaaa
     2821 acatttatat tgatcataac aatctggaaa gtttagattt aaaaaatcag cattcattgg
     2881 ttaatctgga agcgcaaaat aatagcctaa aaaaatgata tttctgatag ttataaactg
     2941 aaatttctta atcttgatta taacaagcta gcatcgctgg atgtctcccg gcaagaatct
     3001 ctgattgagt taagtgccca tcacaatatg atcactgacc ttatattaca caatcatccc
     3061 aggatgaaaa caatcacttt aaacgacaac catattgcac atttaaacgc caaaaacact
     3121 acaaaactgg aatatttaaa cttaagcaat aacaatttac tgccaaccaa tgacattgat
     3181 caactaatat catcaaaaca tctttggcat gtattagtta acggcatcaa caatgatcca
     3241 cttgcacaaa tgcagtactg gactgcagta agaaatataa ttgatgacac taatgaagtg
     3301 accattgatt tatcaggact taatttaacc actcaaccac cagggctgca aaacttcacc
     3361 tctatcaatc ttgataataa ccaactcaca cattttgatg caaccaacta cgatagactc
     3421 gtaaagctaa gtctgaatag taatgctctt gagtcaataa attttcctca aggcagaaat
     3481 gtaagtatta cacatatatc tatgaataat aatgctctca gaaatattga tatagatagg
     3541 ctttcatcag ttacttattt tagtgcggca cataatcaac tagagtttgt gcaattagaa
     3601 tcttgcgaat ggctgcaata cctgaatctc agccataatc aattaactga tattgttgca
     3661 ggtaataaaa atgaactctt actgctggat ctatcccata ataaactaac aagtttacac
     3721 aatgtcttat ttcccaactt gaatacgtta cttattaaca acaatttgct ttctgaaatt
     3781 aaaatattct atagcaactt ccgcaatgtt cagacattaa acgctgctaa caaccagttg
     3841 aaatatataa atcttgattt cctgacttat cttccatcta tcaaaagttt aagactggac
     3901 aataataaaa taacccacat tgatactaat aatacatccg atattggaac tttattcccc
     3961 ataataaaac agagcaaaaa cttaaatttt ttaaatgttt ctgggaagaa caattgacct
     4021 actatgcagc tcatgttatt taatttgttt tccccagcac ttaagcttaa tactggcctg
     4081 gcaattcttt cgcctggtgc atttgaagtt cactctgacg gaatagatgc ggataacgaa
     4141 ttgtttcact atactattaa aaaagcatat accccatata atatacacac ttacaagaca
     4201 gaggaagttg taaaccagag gaatataaaa gttaaaaaca tgacct
//
LOCUS       DAEEGA010000202         4183 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEEGA010000202 DAEEGA010000000
VERSION     DAEEGA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.204"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="KLH46_004124"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="KLH46_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBE0380573.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="KLH46_004125"
     tRNA            complement(324..399)
                     /locus_tag="KLH46_004125"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="KLH46_004126"
     tRNA            complement(406..480)
                     /locus_tag="KLH46_004126"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="KLH46_004127"
     tRNA            complement(597..681)
                     /locus_tag="KLH46_004127"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="KLH46_004128"
     tRNA            complement(690..765)
                     /locus_tag="KLH46_004128"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="KLH46_004129"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="KLH46_004129"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBE0380574.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="KLH46_004130"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="KLH46_004130"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBE0380575.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGEMLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="KLH46_004131"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="KLH46_004131"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBE0380576.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1039 c          930 g         1134 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcgctgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcat ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tag
//
LOCUS       DAEEGA010000203         4110 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEEGA010000203 DAEEGA010000000
VERSION     DAEEGA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.205"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(954..1145)
                     /locus_tag="KLH46_004132"
     CDS             complement(954..1145)
                     /locus_tag="KLH46_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBE0380577.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            1209..1532
                     /locus_tag="KLH46_004133"
     CDS             1209..1532
                     /locus_tag="KLH46_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBE0380578.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1522..1833
                     /locus_tag="KLH46_004134"
     CDS             1522..1833
                     /locus_tag="KLH46_004134"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380579.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVDAPGQGT
                     A"
     gene            1808..2230
                     /locus_tag="KLH46_004135"
                     /pseudo
     CDS             1808..2230
                     /locus_tag="KLH46_004135"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2211..2741
                     /locus_tag="KLH46_004136"
                     /pseudo
     CDS             2211..2741
                     /locus_tag="KLH46_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
     gene            2748..2981
                     /locus_tag="KLH46_004137"
     CDS             2748..2981
                     /locus_tag="KLH46_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBE0380580.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3527..3994)
                     /locus_tag="KLH46_004138"
     CDS             complement(3527..3994)
                     /locus_tag="KLH46_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380581.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT         1001 a          938 c         1194 g          977 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtccctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggtatt tcatacccgg tatggcgcac ttttcataca
      781 atctgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ctgctgatgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacaga caagctgtga gcggatgccg ggagcagcat atgtcagagg
      961 ttttcaccgt catccccgaa acgtgcgagg cagctgcggt aaagctcatc ggcgtggtcg
     1021 tgaaacgatt cacagatatc agcctgttca tccgcgtcca gttcgttgag cttctccagc
     1081 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgttta
     1141 gtcactgatg cctccgtgta agggggattt ctgttcagga ggtaatgata ccgatgaaac
     1201 gcgagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgct
     1261 gtgagggtaa acagctggcg gtatggatgc ggcgggtctg cctgggggag ccggttgccc
     1321 gttccgggaa actgccgaca ctggcaccgc cgttactgtg tcagctggct gccatcggga
     1381 ataacctgaa tcagacagcg aggaaggtga acagcgggca gtggtcttcc ggtaaccggg
     1441 ttcaggtggt ggccgcactg atggccatcg gggacgagct gcgccggctg cgtctggccg
     1501 tcagggaaca ggggacgcgg gatgatagtt aagtttcatg cccggggaaa aggtggtggc
     1561 agtggtccgg ttgattacct gctgggaagg gagcgtaacc gggaaggggc aacggtgctt
     1621 cagggtaatc cggaagaagt ccgggaactc atcgatgcca cgccatttgc gaagaaatac
     1681 acgtccggtg ttctgtcgtt cgcggagaag gagctgccgc cgggaggacg tgaaaaagtg
     1741 atggcgagct ttgagcgtgt actgatgccc ggtctcgaaa aaaatcagta cagcatcctg
     1801 tgggtggatg caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga
     1861 gctacagacc ggaaaacgcc tccagccgta ctacgaccgc gcagacaggc ctagaattga
     1921 tgcctggcag acgctggtta atcactatta cgggctgcat gacccgaacg ccccggagaa
     1981 ccgcaggacg ctgacactcc ctgataatct gccggaaacg aaacaggcga ttgctgaggg
     2041 cgttacacgc gggatagacg cgctttacca tgccggagag ataaaaggcc gtcaggatgt
     2101 gattcaggct ctcacagagg cagggattga cgtggttagg gtgacgcgaa gcagtatcag
     2161 cattgcagac ccgaacggcg ggaagaatat cagactgaaa ggagcgtttt atgagcaatc
     2221 ttttacagac gggtgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga
     2281 aaatgctgaa cgacgagttc aggaagctcg aagaatctgt aaacagggaa ctgacctcaa
     2341 acgaatcttt aatcaggaac gctatcaacg accatacatc agccctgaaa gaacttctgg
     2401 agaggtatca gaaaacgacc gtcgatacga tggacgcaca ctggaaaacc gtactgaaaa
     2461 tgagcgtgaa acgctggctg tggctgatta tcgtctcagt actgatgttt gccacgaccg
     2521 ggagcctgct gtggtaccag ggcatgaaga taaacgcgaa catgaatatt ctccgcgagt
     2581 agaaagagag tctggagaag ctgaacgcga agacatgggg cgtgcgttat cacgaggaca
     2641 gcaacggacg ttttctggtg ttgcccgagg ggatgaaagc cgaaacgaac tggacgatgg
     2701 aaaacgggaa acagaatgcc gtaaggctgg tacaggagta acagagcatg acggagctgg
     2761 aaagacattt gctgaacgca ttagaacaac tgcaacagga ctatatgcag cggctgaacg
     2821 aatgggagag cgccttcgtg gaattgcaga aaatgttttc gcttacgcaa cgggacaacg
     2881 cgatgctgaa cgagcgggtc atgcagttga gtcagcaggt gcagcacttg agcgagcaga
     2941 cagaacgctt gagccagtta tacagcgaga acaggagata agggacgaac ggctgataca
     3001 ggaatgcgaa catgcgttat ccctggagcg tgagcaccag ctggaaatac aggaacggac
     3061 actggatggc ccttcgctgg gatggtaatc agagacaggc taaaatgttg tcagcgcgtt
     3121 tccggtgcat tttttgggat aatggttggt tcgggggcac cgggaacgcg cttcagtcag
     3181 accttgctga aaacatagga acgcgttaaa acgcatacgg gcgcgattct ggtgtgtctt
     3241 ttgttgctgt gttttgcctc tattgccaga tcgcgctcat tcccctgaac ttcttttttc
     3301 ccgctcaggt ccccgtcttc ttcgcttccg aaagcatgaa aaaaatccgt ttcagctgtc
     3361 tctggctggc aattcctgcg tgattcacat ggctgcatag ctatgcatgt ggttaaaatt
     3421 taccgggtgc gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg
     3481 ccgtgatcgc gccgaacgcg tttcagcggt gcgtacaatt aagatattac gttaaattgt
     3541 gagggaatgg tccccatata ctgactttat catagggata gggttggtag tgggtcgatt
     3601 gggtttcggc attgtttccg ttaatgccaa ttgcattttt gtatttgatg caacagaagg
     3661 cgtgagattc tcctgggcct ggaaaggata agtgtttaga attgatggca aaatatcttc
     3721 catccgattt ttgaagtagt tgttgtttgg ctgtctccca aagggtgttg ttacaaactt
     3781 cttgaatttg tagtttttgc aggactctta ttactgcgtt atcatgttcc aattctttag
     3841 gggagtgtat tattctctct tttaataagc tgtttaccac agggtttagt tgttgtctaa
     3901 agcacgcaga taatgccatt gctacacaat tttgttcccg aggaagcatg tttggcgggg
     3961 tatgccgtgt ttgatgtgat atgtctattc tcatagaagg tctctggctt atagcatcaa
     4021 caaatacggc tataaaacaa aattttcata gagtgattca agatcttctt gagatccttt
     4081 tttgcggcgc gtaatctgct gcctgcaaac
//
LOCUS       DAEEGA010000204         3972 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEEGA010000204 DAEEGA010000000
VERSION     DAEEGA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.207"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..221
                     /locus_tag="KLH46_004139"
     CDS             <1..221
                     /locus_tag="KLH46_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBE0380582.1"
                     /translation="QRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPV
                     LQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            267..476
                     /gene="hha"
                     /locus_tag="KLH46_004140"
     CDS             267..476
                     /gene="hha"
                     /locus_tag="KLH46_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBE0380583.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            515..1105
                     /locus_tag="KLH46_004141"
     CDS             515..1105
                     /locus_tag="KLH46_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBE0380584.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1345..1605
                     /gene="repA"
                     /locus_tag="KLH46_004142"
     CDS             1345..1605
                     /gene="repA"
                     /locus_tag="KLH46_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBE0380585.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1829..1903
                     /gene="tap"
                     /locus_tag="KLH46_004143"
     CDS             1829..1903
                     /gene="tap"
                     /locus_tag="KLH46_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBE0380586.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1896..2753
                     /gene="repA"
                     /locus_tag="KLH46_004144"
     CDS             1896..2753
                     /gene="repA"
                     /locus_tag="KLH46_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBE0380587.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3453..3644
                     /locus_tag="KLH46_004145"
     CDS             3453..3644
                     /locus_tag="KLH46_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380588.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT         1072 a          988 c          989 g          923 t
ORIGIN      
        1 ttcagcgaga tcgttatggt cgtattctcg ggcaggttta tgcaccagac ggaatgaata
       61 ttaatcagtt tatggtccgg gccggggctg cctgggtata tgagcagtac aacacagatc
      121 ccgtgctacc tgtccttcag aacgaagcca gacaacaaaa acgtgggctc tggtcagatg
      181 ctgatccggt tcccccctgg atatggaggc accgtaaata aagcgatggc aatcctatca
      241 ttcatctgtt ctcagcggga ataaaaatgg caaaaacaaa acaggaatgg ctgtatcagt
      301 tacggcgatg ttcatcagtc aatacgctgg aaaaaattat tcataaaaac agagacagtt
      361 tatcaaacag tgaacgtgaa tcatttaatt cagcagcaga ccaccggcta gcggaattaa
      421 tcaccggaaa gttatatgac aggataccaa aagagatatg gaaatatgta aggtaataat
      481 tcagcaataa ttaattgcca ggagcagata aataatgaag ttgattattt ttattctcat
      541 tgttctgatt attgcagcgc tacttatccg gattatcctc agaagtgtaa atcagcattc
      601 tcctttgctg atgcaattgc atgcggcagg tatacggact ggtgatgcag agcgaatact
      661 gtccagcggt gaatactggc aacgtcagaa gacgctgctg acagaaaggg aagtcagttt
      721 tatgaaagga ctgttcagaa ttgtggatat gaagcggtgg tatctgtgtc cgcaggtacg
      781 ggtagcggat atcgtccagc tgaacgggaa tatccggcca cgatcacgcc agtggtggca
      841 gttattcagg atggtgtctc agtggcatgt tgatgtggtt atcgttgagc tgcgttcgtt
      901 cagtattgtt gcagcagtag agctggatga tgccagccat ttacgaccgg aacgcagacg
      961 ccgggatatt cttctggaag aggttctgag gcaggctggt attccgttgc tcagaagcca
     1021 cgatgccaga aaactgctgc agatgaccgg agaatggctg aatacaacag gggctgatca
     1081 gcagtcaccg gaacatcgta gctgacgcct tcgcgttgct cagttgtcca accccggaaa
     1141 cgggaaaaag caagttttcc tcgctcccgg cgtttccata actgaaaacc atactatttc
     1201 acagtttaaa tcacattaaa cgacagtaat ccccgttgat ttgtgcgcca acacagatct
     1261 tcgccacaat tctcaagtcg ctgatttcaa aaaactgtag tatcctctgc gaaacgatcc
     1321 ctgtttgagt attgaggagg cgagatgtcg cagacagaaa atgcagtgac ttcctcattg
     1381 agtcaaaagc ggtttgtgcg cagaggtaag cctatgactg actctgagaa acaaatggcc
     1441 gctgttgcaa gaaaacgtct tacacacaaa gagataaaag tttttgtcaa aaatcctctg
     1501 aaagatctca tggttgagta ctgcgagaga gaggggataa cacaggctca gttcgttgag
     1561 aaaatcatca aagatgaact gcagagactg gatatactaa agtaaagact ttactttgtg
     1621 gcgtagcatg ctagattact gatcgtttaa ggaattttat ggctggccac gccataaggt
     1681 ggcagggaac tggttctgat gtggatgtac aggagccaga aaagcaaaaa ccccgataat
     1741 cttctttaac tttggcgagt cagaaagatt accggggccc acttaaaccg tatagccaac
     1801 aatcaagcta tgcggggagt atagttatat gcccggaaaa gttcaagact tctttctgtg
     1861 ctcgctcctt ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa caaacttatt
     1921 accgccaggt aaagaacccg aatccggtgt tcacaccccg tgaaggtgcc cggacgcttc
     1981 cgttctgcgg gaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt gatttcgcca
     2041 ttcatgtggc acatgcccgt tcgcttggtc tgcgtcggcg catgccaccg gtactacgtc
     2101 gccgggccat tgatgcgctg ctgcaggggc tgtgttttca ctatgaccca ctggccaacc
     2161 gcgtccagtg ttccatcacc acgctggcca ttgagtgcgg actggcgacg gagtctgctg
     2221 ccggaaaact ctccatcacc cgggccaccc gggccctgac gttccttgca gagctgggac
     2281 tgattaccta ccagacggaa tatgacccgc ttatcgggtg ctacattccg accgatatca
     2341 cgttcacatc tgcactgttt gctgccctcg atgtatcaga ggaggcagtg gccgccgcgc
     2401 gccgcagtcg tgtggaatgg gaaaacagac agcgcaaaaa gcaggggctg gataccctgg
     2461 gtatggatga actgatggcg aaagcctggc gttttgttcg tgagcgtttc cgcagttacc
     2521 agacagagct taagtcccgt ggaatgaagc gtgcccgtgc gcgtcgtgat gcggacaggc
     2581 agcgtcagga tattgtcacc ctggtgaaac ggcaactgac gcgtgaaatc tcggaagggc
     2641 gcttcactgc cagtcgtgag gcggtaaaac gtgaagtgga gcgtcgtgtg aaggagcgca
     2701 tgattctgtc acgtaaccgt aattacagcc ggctggccac agcttccccc tgaaagtgac
     2761 ctcctcagaa taatccggca cgcgccggag gcattcgcac gcctgaagcc cgccagtgca
     2821 caaaaaatca gcgctacata caaaaaacaa cctcatcatc caccttctgg tgcatccggt
     2881 cccccctgtt tttgatacaa aacacgcctc acagacgggg aattttgctt atccacatta
     2941 aactgcaagg gacttcacca taaggttaca accgttcacg tcataaagcg ccagacgtca
     3001 accctacagg gtgcaatgta tcttttaaac acctgtttat atctccttta aactacttaa
     3061 ttacattcat ttaaaaaaaa aacctgttca ctgcctgtcc tgtggacaga cagatatgca
     3121 cctcccaccg caagcggcgg gccccaaccg aagccggttt agttacaaca tgcaaacata
     3181 aacccctaaa aaacccaagt ccaacacaga actgaaacca caacgccccc ctcataactg
     3241 aaaagcgccg ccgcccccgc ccaaagggcg ggaacagagt cgctattaat tatgaatgtt
     3301 gtaactacat catcatcgct gtcagtcttc tggctggacg tatcgagtac acgctcgtaa
     3361 gcggccctca cggcccgcta acgcggagat acgccccgac ttcgggtaaa accctcgtcg
     3421 ggaccactcc gaccgcgcac agaagctcta tcatggctga aaacgggtat ggcttggcag
     3481 ggctggggat gggtaaggtg aaatctgtca atcagtaccg gctgacgccg ggcttcggcg
     3541 gttttactcc ggtatcacat gtaaccacag cgtgccgcct tccatgccgc tggcgcggca
     3601 tcagaataat acaagcagca tttaatgcat ttgcaaaagt gtgagtaata ttaagtggtc
     3661 gtttcaggga tatcgttaag acttgagcga taggaattaa aaccccaaaa agattaaaaa
     3721 aacaccacaa aacggatgtt tcttcaacac cacttttgct ccatatgaac ggaaccgacg
     3781 attaaactgg atggctctga ttgattcagg gtatgaatgg cggttttttg ctccgtttcc
     3841 ctcaaaatgg acgcaacttc ccctctgcgg ctctcagccg caccaccgca tccgggccag
     3901 cggctcatgc atcaggacct gctctgccag acggtagccc cgcttcagcc ccgtaaaacg
     3961 catctgactc cc
//
LOCUS       DAEEGA010000205         3934 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEEGA010000205 DAEEGA010000000
VERSION     DAEEGA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.208"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..178)
                     /locus_tag="KLH46_004146"
     CDS             complement(<1..178)
                     /locus_tag="KLH46_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0380589.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTG"
     gene            231..685
                     /locus_tag="KLH46_004147"
                     /pseudo
     CDS             231..685
                     /locus_tag="KLH46_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            709..1117
                     /locus_tag="KLH46_004148"
                     /pseudo
     CDS             709..1117
                     /locus_tag="KLH46_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912930.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1701..2417
                     /locus_tag="KLH46_004149"
     CDS             1701..2417
                     /locus_tag="KLH46_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380590.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2969..3373
                     /locus_tag="KLH46_004150"
     CDS             2969..3373
                     /locus_tag="KLH46_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380591.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            3436..3934
                     /locus_tag="KLH46_004151"
                     /pseudo
     CDS             3436..3934
                     /locus_tag="KLH46_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1097 a          836 c          941 g         1060 t
ORIGIN      
        1 ccccggtatc ccgctcatgc tggcgaaccc agacacgcag agtctccggc gtacagccaa
       61 tctttggagc aatggaacaa attgtcgccc attgtgagtc atattcgccc tgactttcca
      121 gaaccatacg gactgcccgt tgacggactt cgggggaaaa acgagtattt ttagtcatcc
      181 tgtttacctc tttctcagga agtttagtct ccaggattcc cggggcggtt cagaatgaac
      241 atccgcatct tcctgttgaa agtcgtcgtc aggcaaaggg aactcgcttc ctgtggcagc
      301 acagcgataa atggatgacc gaatccaggc aggaaaagct gatatggctg cgtgcacaga
      361 tgaagctgac gagcctgtgc tgggcgctga aagaactggc aaaggatatc tggagcagac
      421 catggagcga ggaaagacgc aacgactggc agagatggct gcggccaaca gtgacgtccc
      481 catgatgaag aatgtggcta aaacgatagg gaaaaggttg tacggtatcc tgaacgccat
      541 gcgccatggc gtatcaaacg gaaatgcgga agcactgaac agcaagatca ggctgctgag
      601 aataaaagcc aagggctacc gcaaccggga gcgctttaag ctgggagtga tgttccacta
      661 tgggaaactg aatatagcgt tctaagcctt cccaccatga tcggggaaga cccagatatg
      721 aacaggcaca ttcagagtta ctttaaagag cttactgaac gtctgaggtg ttagagccat
      781 gactgccgca gcattgctgg cggaaatgcc tgaatcgagc agcctctcga gacgtgaaat
      841 cagcgcactt gtcggtgttg ctcaggtaaa ccgggactcg ggaactctgc gcggtcgacg
      901 aaccattttc ggtgattgtg ctggggaaga acagctctgt acatggcggc gcttgaggcc
      961 cccccggttt aatctggtga taaaggcgtt ttacatgcgc ctacttgcag ccggaaacgc
     1021 caaaaaagtg gcactggttg cctgtatgcg taagttgctg accattatga acgcaatgct
     1081 cagaaaaaac gaagaatgga atgaatcgta tttatgaaaa ctggattgtg ggccataaat
     1141 attccaaaaa gatagcccgc tccgtttatt accggaacgg gcgagagcat gacattgtct
     1201 gttattataa tgagactgct gattctactt atttttaatt tcaagaaaag cgttttctac
     1261 aaagaattat gagatttaat gttttgttta gacaatatta tcattctgtt caataatgaa
     1321 agtcttcatc aacaacaagt aaaaacaacg tccctctcat cgaaccggac ttgcagattt
     1381 cccgcatccg gctctcttgc aacacatcag accttcacgt tcgggcggtt atggggaagg
     1441 tccagcaagc tgtatgagcc ctaactgttt gaataccagt agattaccag aaacttctat
     1501 cgattgaaga cattaccaac tagcgttact aaatgttgac caagttttgt tcacttgtct
     1561 ggttttgata gtagcattct gcagagtatt ttgatatgat aaatatgcat taacaatgat
     1621 attattgtta atttcctagc atgaaaatta aaagatcaac ttttatctcg aatatttttt
     1681 acattatcag ttggtttctt atgaatgaca attcattgct tagaaattct tcattattca
     1741 tcgcctatat gggatgtgta gggtgggtca gtgcttattc ttatggctgg ggaacctctt
     1801 tttactatgg atttccatgg tgggttgttg gtgcaggttt ggatgatgtt gccagaagtt
     1861 tactctatgc tatcatagta atgggtatac tttttaccgg ttgggggatt ggtattttat
     1921 tttttcttct tataaagaaa agaagtaaaa tacaagactt gagttttttt agactatttt
     1981 ttgctattac cctactgttt ttccctgtaa tattcgaact tttaattctc aaacaatatt
     2041 tcatacttcc attatcactt tcattcatta ttagttcatt ggttatttca ataataattc
     2101 gtatatatgg acggatcttt tctgtatcat gtttttcaga tatccctttt gttagagaac
     2161 accgcattaa gttaattatg gctggttttt tggtttattt ttggtttttt tcctttcttg
     2221 ttggctggta taaacctcaa ttaaaaaaag aatatcagat gctttgttat aataatagtt
     2281 ggtattatgt tcttgctcgt tatgattcac ggctcgtttt atctagttca tttaaagatg
     2341 acagtaacag attcttaata tttaatacag aacagtcggg attttatgaa atcaatgatg
     2401 tttatgtcag aaagtaataa ccctctccca caaagtgaat taaatgaata tataatgcgt
     2461 tcgaagtata tgtattgagc tgtcatactg agaaacaaac cttagggcag cacaattaaa
     2521 gccagaaaca actatgcaag aaagttacta aataagtgct cggaacagta aaacactcag
     2581 gggggactca gtccggatta tgtaatcaga taaattgcca aacatcacaa atcaccagcc
     2641 ggactgagtg atgccgagca ttgcaccaat accccgtgat gaacgatgcc tgatgcagaa
     2701 agctccctat aaaacaaaga acaaaaacta cgaccggcga ttcaccgtca tactgatgct
     2761 gcatcgtggt gacactgtct gtcactttca tgttgactga acaactctgc tgcgaagaag
     2821 gtgtaccgcc ggcgggattt gattgtccgg cgtctagctt ccacttccct gatggaactg
     2881 aaagttgtta tggccggtca gcttgccctg tccgcaggac atgaggtgct gtgacggaca
     2941 gcaccagtaa cggaaagtga caatgaaaat gaatactgcg ctgatagttg cactaatgtg
     3001 tatgtggtat gcggtacccg ctgctgcaaa agagacttta ctggctatgc cacgtaattc
     3061 aacagaacat tgctatgcgg agattaatgt gcacggacca tatggggtgt atttccgagt
     3121 ggtcccgcat ccccccgggg gaaagagctg ggtcgaatgt aacagtgact actattactc
     3181 tgataaacca ccgggagtac agatactcgg aacccgtgca gggtgtcggg tatacggaat
     3241 ctgcgggacc acctcgacac tgcatgtggc tggaagaggg gttgtgtgta ttaaaaatat
     3301 atgcagtcca cgtgggatga taattcacag gatacggaaa cgtcctgtag tggctgtaag
     3361 cgatgagatg tgatcttacc cagcaatagt agacacgcgg ctaagtgagt aaactctcag
     3421 tcagaggtga ctcacatgac aaaaacagta tcaaccagca aaaaaacacg taaacagcat
     3481 tcgcctgaat tttgcagtga agccctgaag cttgctgaac gcatcggtgt tgctgccgca
     3541 gcccgtgaac tcagcctgta tgaatctcag ctctatgcgt ggcgcagcaa actgcagcaa
     3601 cagatgacct cgtctgagcg tgaaagtgaa ctgcccgcct gaagcggcaa ctggcggagc
     3661 aggcagagga actggctatc ctccaaaagg ccgcgacata ctttgcgcag cggctggtat
     3721 acgtggtgtc agcgtcggac aggaataagc cctcgtcagc agttccgtca gcactgcgac
     3781 agcgttgtcc tcgcggcggc ctttacccgg tcaaaacagc gttacggtgc accacgcctg
     3841 acggatgaac tgcgtgctca gggttaccac tttaacgtaa aaaccgtggc ggcaagcctg
     3901 cgccgtcagg ggctgagggc aaaggcctcc cgga
//
LOCUS       DAEEGA010000206         3777 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEEGA010000206 DAEEGA010000000
VERSION     DAEEGA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.209"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            32..493
                     /locus_tag="KLH46_004152"
     CDS             32..493
                     /locus_tag="KLH46_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBE0380592.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..1004)
                     /locus_tag="KLH46_004153"
     CDS             complement(534..1004)
                     /locus_tag="KLH46_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBE0380593.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="KLH46_004154"
     CDS             complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="KLH46_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBE0380594.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="KLH46_004155"
     CDS             complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="KLH46_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBE0380595.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          993 a          975 c          858 g          951 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctgacgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatgtcat cggttttcag cgaaaacgcg
      721 atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac gttccggctc cagtgccgga
      781 gcagactcat ccttgccgcg tttttcataa atcaggccat tgaggaatga cgacaaaaca
      841 atgtccggac aacgctgaaa accctcttcg tcttctttac gtagccagac ggcgatctgt
      901 tccgcggtgg cttcgacatt atccagcgcc agaatacgca ccaggtcatt attattggct
      961 ttcaaaatgt agcgcacgct gcgcagaata tcgttactta tcatgaggcc ttcaggtgtt
     1021 gatgaggcaa aaagccattt tagcagtctt ttacaggcca atcgcctctt ttaagctttt
     1081 cagataacgg cggctgaccg gcacggttaa gccattacgc aaaatcaact cggcctggcc
     1141 gttatcttcc agacgaatct cctgtaaatg cgcgaggtta accagatact gacgatggca
     1201 gcgcagtagt ggtgtacgac tttccaaggt acgtaatgtc aattcggtaa agccctcttt
     1261 cccttcgtgg ctggtaacgt agacaccgct catccgactg ctgacaaatg ccacatcttt
     1321 catttgcagc aaataaatcc gactatgccc cgtacaaggg ataaatttca gcgcctgttg
     1381 attttccggt aacagcgaaa catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt
     1441 tttctccagt cgcgcttcat caattggctt cagcagataa tcaaaggcat gttcttcaaa
     1501 ggctttaata gcgtattcgt caaacgcagt gagaaaaaca atatacgggc gatgttccgg
     1561 gtcaagcatc cccaccattt ccagaccact gatgcgcggc atctggatat cgagaaacag
     1621 cacatccggg cgcagtttat gcaccgcgcc gatcccttcc acggcgtttg aacactctcc
     1681 aacgatttca atatcgctct gctcctgcaa aaatatacgc aggttctccc gtgctaacgg
     1741 ttcatcatcg acaattaaga ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt
     1801 attcgggtgt aactatcagg ctcacaggcg acgcttattc catagtcatc gccaaaccgt
     1861 tcacgtaaac gcttatccac cagattcatc cccagcccac tggcattggt taccggttga
     1921 tacaaaccgg cattgtcttc gatctccagc atcaaatgtt gcccctcacg tcgggcgctg
     1981 attgccactc gccctgtatc cagcagttgt gatgtcccat gtttaatggc gttttccact
     2041 atcggttgca gggtaaacgc gggcaattgc tgctgggata attcttgcgg aatagcaatg
     2101 ttgacctgca accgcgactg gaagcgcgcc ttttcaattt gcagataagc gttcacatgt
     2161 tcaatttcgt cggcgagagt aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa
     2221 gtggaaagat actgcaccag ctggctggcc tgttcgctgt cgcggcggat caccgcttta
     2281 atggtgttaa gcgcattaaa caaaaaatga ggattcacct gggcgtgaag cagtttgatt
     2341 tctgactggg tgagcatcgc tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa
     2401 agcagttgcg caatcccctc gcccagcgtg cggtttattg aactgaataa ccggtttttg
     2461 gcttcataca atttgatggt gcccatcacg cgctgatttt cgccacgcaa cggaattacc
     2521 agcgtcgacc ccagtttgca ttgcggatgc aaagagcaac gataaggtac ttcgttgcca
     2581 tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt
     2641 ttgccgggta aatggtggtc gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg
     2701 gtaatcgcga ctgcaccaat atccagctcc tgatacagta cctgagccac tttcatgctg
     2761 ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca
     2821 gtggcagaaa aagccgaagt gtatttttca aacatcgcgc gtttatcgag caatatacgc
     2881 ataaacagcg ccgcgccgac ggtattggtg accatcattg gcgcagcaat attactcacc
     2941 agacgcaccg catcttcata aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt
     3001 tcagcgacga acgtgacggc accggcggta atggggttaa agactttatc agtgcgcccg
     3061 cgacggatca ggatgctgtg taccaggccg ccgagcaatc cttcaacgat ggttgagatc
     3121 atgcaactta gcgcggtcat gccccccatc gaatatcgat gtaagccgcc ggtcagacca
     3181 accagcccac cgacgaccgg accgccgagt aagccgccca ttaccgcgcc tatcgcacgg
     3241 gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg
     3301 gaaaagacga tgtagcagag aaatttatgc ggcagacgaa ccgtgacctg cattaacggt
     3361 atgaataatg gcgttttact cattaaccac gcaatgacta aaaaaacgca catctgctga
     3421 agcagcagca acaccagatt aaaatcgtac atacccgcaa accacacttc cctttaaaac
     3481 gcgtaacata cattgcctgc gtttaacttt ctttgaactc ttgcagaaaa atgagaattc
     3541 gtgagtacga tcactcaaaa tcgcctggca aaaataaaat caccctatag atgtaatgcc
     3601 gatcagttaa ggatcagttg accgatccag tggctgtgta agaatccgga aacgctcact
     3661 tgtttccgga tttttttatg cacattggac aggctcttga tctggtatcc cgttacgatt
     3721 ctctgcgtaa cccactgact tctctggggg attacctcga ccccgaactc atctctc
//
LOCUS       DAEEGA010000207         3613 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEEGA010000207 DAEEGA010000000
VERSION     DAEEGA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.210"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(226..429)
                     /locus_tag="KLH46_004156"
     CDS             complement(226..429)
                     /locus_tag="KLH46_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380596.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="KLH46_004157"
     CDS             complement(431..586)
                     /locus_tag="KLH46_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBE0380597.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="KLH46_004158"
     CDS             complement(789..1196)
                     /locus_tag="KLH46_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0380598.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="KLH46_004159"
     CDS             1273..1500
                     /locus_tag="KLH46_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE0380599.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="KLH46_004160"
     CDS             1484..1906
                     /locus_tag="KLH46_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380600.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2767
                     /locus_tag="KLH46_004161"
                     /pseudo
     CDS             1984..2767
                     /locus_tag="KLH46_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2774..3520
                     /locus_tag="KLH46_004162"
                     /pseudo
     CDS             2774..3520
                     /locus_tag="KLH46_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3535..>3613
                     /locus_tag="KLH46_004163"
     CDS             3535..>3613
                     /locus_tag="KLH46_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBE0380601.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGR"
BASE COUNT          927 a          788 c          990 g          908 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagattttta tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gttggcaagc attgctgcgt ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccttggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagaggaaga tctaaatact gatctccccc taaatccccc tcgccaaaaa
     2461 cgagcgtcta aaaaattcga gccggaggct attgagctgc ccgattggtt gccggaaaca
     2521 ctctggcatg agtgggtccg gttcagacag gcattgcgaa aaccgattcg aacggagcag
     2581 ggcgatacgg gaactggaaa aattccgtca gcagggtttt acacctgagc aggtgattcg
     2641 acacagcatc gccaatgaat accagggcct gttcgcgccg aaaggtgttc ggcctgagac
     2701 gttgctccga caggttaaca ccgtctcgtt gccggacagt gcgatcccgc caggcttcag
     2761 ggggtaacgg accatgaaaa atattgcgac aggcggcgtt ctggaacgca tccgccgact
     2821 gaccccgcaa catgtaaccg ccccattcag aacggtagcg gagtggcgcg agtggcaact
     2881 tgctgaaggc cagaaacgta gcgaggagat caaccgcctg aatcgccagt tgcgggtgga
     2941 aaaaattctg aatcgctcag gcatccagcc gttgcaccgt aaatgctcgt ttgcgaatta
     3001 ccaggtgcag aacgacggcc agcgatacgc gttaagccag gcgaaatcca tcgccgatga
     3061 actgatgacc gggtgtacaa attttgcgtt cagcggaaaa cctgataccg ggtagaatca
     3121 cttagcggca gctatcggga atcgcctgct gaaagacggt cagacagtga ttgtggttac
     3181 cgtggctgat gttatgagcg ccctgcacgc cagctatgac gatgggcagt caggcgaaaa
     3241 atttttgcgg gagctgtgcg aagtggatct gctggttctt gatgaaattg gcattcagcg
     3301 cgagacgaaa aacgagcagg tggtgctgca ccagattgtt gatcgccgga cagcgtcgat
     3361 gcgcagcgtg gggatgctga caaacctgaa ctatgaggtg atgaaaacat tgctcggcga
     3421 gcgggtgatg gatcgcatgg tcatgaacgg cgggcgctgg gttaatttta actgggagag
     3481 ctggcgtccg aatgttagcc attcgagggt tgttaagtag tttcaggagg atttatggcg
     3541 aaacctttta ctcccgaaca gcgggaagaa ctgaagacgc gaattgtgga actcgtgcat
     3601 caggacggtc ggg
//
LOCUS       DAEEGA010000208         3610 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEEGA010000208 DAEEGA010000000
VERSION     DAEEGA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.211"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(153..419)
                     /gene="ospE2"
                     /locus_tag="KLH46_004164"
     CDS             complement(153..419)
                     /gene="ospE2"
                     /locus_tag="KLH46_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HBE0380602.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(601..1002)
                     /locus_tag="KLH46_004165"
                     /pseudo
     CDS             complement(601..1002)
                     /locus_tag="KLH46_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1431..2219
                     /gene="virF"
                     /locus_tag="KLH46_004166"
     CDS             1431..2219
                     /gene="virF"
                     /locus_tag="KLH46_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HBE0380603.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(2212..2583)
                     /locus_tag="KLH46_004167"
                     /pseudo
     CDS             complement(2212..2583)
                     /locus_tag="KLH46_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2582..3336
                     /locus_tag="KLH46_004168"
                     /pseudo
     CDS             2582..3336
                     /locus_tag="KLH46_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1030 a          731 c          744 g         1105 t
ORIGIN      
        1 taagttggca gcattaccgt atcgaggata gatggagtta ctcctctctg aaatagaatt
       61 actgctctac tttttctgtt tccagaaatt attagtaaca aaccctgata tattcttttg
      121 aaaatggagc ttaaacgagt ctgaaattaa cttcagaaat cagaataccg ttggctttgt
      181 ttttcatcaa ttccggcttc atttatcttt gtcaaccatt gcatttctgt agcagtgaat
      241 ttcaacgttt tcgtccggtt ggcagcaggt atttgtaatt cgtatatagc tgctttttta
      301 ttgaaaacag tatatccatc tgtagtaaca gtagaagata ataatacagg atttgatact
      361 tcaagaatga tattttcttg tttttgtgga aggcaaggga atatagtttg tgtaagcata
      421 ttgcttcctt atgttatatt ttagtaaaga atttattttt tacattatag tcggctaaat
      481 gaagtttact gtaaaaaaac gatacttttt tcgtaagcga tgagatgtgg agccagaaaa
      541 atggctgcgt tacgtcattg aactgtcctt gacccacgat attcagacag tcgttgtatc
      601 ttaagttgac attacccgct gacgtcgata ttcccttggt gagagataac ccaacgcact
      661 atgcggatgg ttttcattgt aatgctcgat cgccactgca aggttatgca atgccgttct
      721 tggtttcggc atgaacgcga tgcagtcttc cttcatcgtt ttcatgaacc gttctgctat
      781 tccattactc tgagggctac tgactgccgt cgtgcagggc tccagattca actctctggc
      841 gaactgccgt gtttcatgag ccgtataagc cgaaccgtta tcggccactg gattggccgt
      901 tccggtcctt tatcgccata tcgctcctga catgacatcc tgcaccgttg ctttgtcata
      961 atctcccgtg cctgctgccc agtctatggc ttcacgatcg caatatggaa cgtaacgctg
     1021 tcatcgggag aatcgtcaat gcggcattcg gttaacgctt accaatatca acaccaaccg
     1081 gatttgtgga gcctccagtc tgaaggggct ttatgcgttc cgtataggat attatgatgc
     1141 tggagttttg cgaagctcca ctcctttccg aacatttaca cagatattgc ctaagaaaag
     1201 tgaaaccata atatggttat agtccctttc agtgcaaata cttagcttgt tgcacagaga
     1261 aatagaagct gcataagctc tttcttcaaa aaatgtaaat aaagttaaat ataggaaaaa
     1321 ttacttaatc tatcttaata acggaaagat tttgtataca atcactgtta cagaaatttc
     1381 ttagttactc tgtaaacact aaatatagtt tggtatattc tgttgaattt atgatggata
     1441 tgggacataa aaacaaaata gatataaagg ttcgcttgca taactatatt attttatatg
     1501 caaaaaggtg ttcaatgacg gttagctcag gcaatgaaac tttgactatc gatgaagggc
     1561 aaattgcttt tatagagcga aatatacaaa taaacgtctc cataaaaaaa tctgatagca
     1621 ttaatccatt tgagattata agccttgaca gaaatttatt attaagcatt attagaataa
     1681 tggaaccaat ttattcattt caacactcct attctgagga gaaaaggggg ttaaacaaaa
     1741 aaatattcct cctctctgag gaggaggttt ctatcgattt gttcaaatct ataaaagaga
     1801 tgcctttcgg caaaagaaag atctatagtt tagcttgcct tttatcagct gtttctgatg
     1861 aggaagcttt atatacttcg atatcgatag cttcttctct tagtttttct gatcagataa
     1921 ggaagattgt tgaaaaaaac atcgagaaga gatggcgtct ttctgatatt tcaaataact
     1981 tgaatttatc agaaatagct gttagaaaac gattggagag tgaaaaatta acatttcaac
     2041 aaatccttct tgatattcgc atgcatcatg cagcaaagct tttattgaat agtcaaagct
     2101 atattaatga tgtatcaaga cttatcggaa tatcaagccc atcttatttt ataaggaaat
     2161 ttaatgaata ttatggtata actccaaaga aattttactt atatcataaa aaattttaaa
     2221 tgcttcatag cccatcgcta ttgtcagatg ggttttcccc acacctgatg gccccagtaa
     2281 tacgatattt tcattacgtt ctatgaagct gagtgagcgt aacgactgga gttgcttctg
     2341 cggtgctccg gtggcgaatg tgaagtcata ctcttcgaac gttttcaccg ccgggaaggc
     2401 tgccattcgg gtatacatcg cctgtttacg ttgatgacgt gccagttttt cttcatgaag
     2461 cagatgctcc aggaagtcca tataactcca ttcctggtct actgcctgtt gtgacagcgc
     2521 aggcgctgcg cttatgtact gcacccattt tgttggacga tgaaatggaa tagcccctga
     2581 tatgtcaaag ccaaaatacc cttttgaaaa gcgccttgaa gtcgtgaatc actacttcac
     2641 tactgatgat ggttacagga tcatctcggc acgttttggt gtcccccgaa cccaggtcag
     2701 gacatgggtt gccctctatg aaaaacatgg agaaaaaggc ttaattccca aacctaaagg
     2761 tgttagtgct gatccagagt tgcgtattaa ggtcgtgaaa gctgtgattg agcagcacat
     2821 gtcccttaat caggctgctg ctcactttat gcttgctggt agtggttctg tagccaggtg
     2881 gctgaaggtt tatgaagagc gcggagaagc tggtttacgc gcgctcaaga ttggcaccaa
     2941 aagaaacatt gcaatatcag ttgatccaga aaaagcggca tcagcattgg agctgtcaaa
     3001 agaccgacgc attgaggatc ttgaaaggca agttcgattt cttgaaacgc ggcttgtgta
     3061 tctaaaaaag ctgaaagcct tagctcatcc cacgaaaaag tgaaagtact caacgagcta
     3121 aggcagtttt atcctcttga tgagcttctc agggctgcgg agataccgcg cagtacgttt
     3181 tattaccatc taaaggctct cagcaagcct gacaagtatg cggacgttaa aaagcgtatt
     3241 ggtgagattt atcatgagaa tagaggccga tacggatacc gtagggtaac gctgtctctt
     3301 catcgagaag ggaaacagat taaccataaa gctgttcacg tataggaaat tgaaaaacta
     3361 agcgataccc ctgtccgcgc cttatgccac gtgagctttt ctgcttttcc ctccactccg
     3421 gtgttttctc tctctgcatg gtcgattctt ataccgcaac aggctgtttt ttatacttgc
     3481 accgattccc gggcacactt agcctgtgtc aggccggcat atacctcagc agactgatat
     3541 cccgcgcgtt tttcttcagc ccctccacat tcagtcccgc gatggcccgg cggtatacca
     3601 tccggccgcc
//
LOCUS       DAEEGA010000209         3550 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEEGA010000209 DAEEGA010000000
VERSION     DAEEGA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.212"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(85..297)
                     /locus_tag="KLH46_004169"
                     /pseudo
     CDS             complement(85..297)
                     /locus_tag="KLH46_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..876
                     /locus_tag="KLH46_004170"
                     /pseudo
     CDS             572..876
                     /locus_tag="KLH46_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            1045..2409
                     /locus_tag="KLH46_004171"
     CDS             1045..2409
                     /locus_tag="KLH46_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0380604.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSRPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGVSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
     gene            2459..2668
                     /locus_tag="KLH46_004172"
                     /pseudo
     CDS             2459..2668
                     /locus_tag="KLH46_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(2762..2953)
                     /locus_tag="KLH46_004173"
                     /pseudo
     CDS             complement(2762..2953)
                     /locus_tag="KLH46_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2994..3491)
                     /locus_tag="KLH46_004174"
                     /pseudo
     CDS             complement(2994..3491)
                     /locus_tag="KLH46_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          868 a          802 c          843 g         1037 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcagtgaacc tcgatcggtg tggtggatta gcccggcagg
      121 tgggcgctgg ctcctgagcg ccataaacag ggctttaccc gtcaactctt tcgtcatgcg
      181 ctctcccatg gcgtaaccga caatttcgca cgtataaaca tctttgacgc cagcgagata
      241 cagccatcct tcccgggtgg caacatacgt tatatctgcg acccagacct gattaggggg
      301 gttggggagc aatggaacag taaacgccgt taagaagtca atttaaatag ccaaaaatta
      361 actagccaat ttacattgca ggttgtaatt tttaaccacc ttttttacga tagccaaatt
      421 acctataccc atcccggccc tgccaagcca tacccgctat cggcgatgaa aatgcttttg
      481 tgcgcggtcg gagtggtccc gacgagggtt ttacccgaag tcggggcgta tctccgcgtt
      541 agcgggccgt gagggccgct tacgagcgtg tatgagagac gtacagtggt tttgtccgct
      601 cacaccagta catacaggta cggaaataaa accagtcgaa atgccggtaa cgacgattac
      661 tcctgtctct gatgccggtg gactacaaga ttttatttac tggcgtcctg atgcggacag
      721 gactggtatg gaacctgttt atgtgatact taatgatcct ctggattccg ggcgatttac
      781 gcgtaaacaa ctaaataaaa atatcttaaa catgcaagta attttggtat tctcgataca
      841 agaataaata gtagtaacta ctatagttgc aaataatgtt aaaaacattt ctatgatcat
      901 ctttacattt ttcatgattg acaattgttt ttggttaaaa aaagacaact aatgagtgtg
      961 ataatgcaat actttgagtg attatataat gataagtaaa caggtttgcg acaattggtt
     1021 atcagtatta cagaaggatt aaatatgtca ttattcacag aacctaagga aattgaaaga
     1081 ttaccctctg aagagattga acgattatat cccgtcctgc gatacagggt ctttatcagc
     1141 attttcctcg gatatatggg gtattatttt gtccgaaaca ccaccagtgt gttatccggt
     1201 gtgctgcaca tgtctgcaac cgagatcggc attatctcct gtgccggttt tttatcctat
     1261 ggtatatcaa agtttgtttc cggacttatt tctgacagaa gtaattcaaa agtatttttg
     1321 tctcttggtt tattcctgtc cggactggta aattttctga taggatatat tcccgggatc
     1381 atcacatccg ttacgttatt ttcaacaatg tatttactta acggctggat ccaaggtatg
     1441 ggatatcctc ctggtgccaa aacactggtg ttctggtatg aacatcggga aaggatcacc
     1501 tgggccacac tgtggaattt atcgcacaat gtcgggggag ctctggcccc cgttctgatt
     1561 ggtttcagtt ttggtttttt cggcgacagt gctctggatc atgcccgggc cgcatttatc
     1621 ttcccaggag tactgtgtat ggcaatgagt gtgctgattt actttattca ggtggacaga
     1681 cctgtatcag tggggcttcc tcccattgaa gaatggaaag gtaacgtcgt ttcacgcccg
     1741 gctaaaggcc gtgaacaagg tccgagactc tccattcctg atatcatcag aaaacatatc
     1801 atccgaaata ataaactcat ttactgctgt atctatggtt cgtttgttta tattctgaga
     1861 tacggtattg tttcctgggc tcctaagttt ttaagtgaca gtctggatgt cggtggtaag
     1921 gatatgggaa aactggcatc aatggggggg gtttctgttt ttgaaatcgg tggtgtggct
     1981 ggtatgcttc ttgccggata cctgtctgtc agactgttca gaaactcaaa accattaaca
     2041 aatactttat ttctggcact taccattatt ttattaatcg cgtactggta cgtgccatca
     2101 ggcaatgaat atctctggct aaactataca atccttattc tgttggggct ggcagtgtat
     2161 ggtccggtga tgtttattgg tctttattca atggagcttg ttcctaaaga agctgccgga
     2221 gccgcatcag ggctgtcagg cacattttcc tatatcttcg gctcaattgt tgcgacactg
     2281 ggaatgggac ttgttgtcga ttatctggga tggggggcca catttatcgt actgatactg
     2341 tctgctgttt ttgccatcat tttcacactg atgagtcgtg aacgctcact ggagttcgaa
     2401 aaagagtaat tttctcagga aaaacataaa attactgact aaatatttat tttttgaatc
     2461 gcagggcatt ccggataagg ttgatatcgt gtcacggctg aagacctatg gatacagtat
     2521 ttctggtgtg gagacggatg acggatataa ggcgcttgtc cgcgcttttc agcttcattt
     2581 ccggcagaaa aattatgacg gcataatgga tgctgaaacc gctgccatcc tgtatgcatt
     2641 gctggagaag tatttcccgg gtaaataagg agtgagtcgt cagagctgcg atatatctcc
     2701 ttcctccatg ggctgtctct tatacacaaa tccccacccg gggatttgtg atgtcagatg
     2761 tccccagtaa gactatgttt tcattgcgct ctatgaagct gagtgagcgt aacgactgga
     2821 gctgcttctg cggtgctccg gtggcgaacg tgaagtcata ctcttcgaac attttcaccg
     2881 ccgggaaggc tgccattcgg gtatacatcg cctgtttgcg ttgatgacgt gccagttttt
     2941 cttcatgaag catgatgctc caagtaaacg ttcaccgaac accgtattga tgattatcct
     3001 gatggtagca ccaccttcca tggcaggagg tcgtcaactc ggttggaagg ccattccggc
     3061 agtacgctca gaatatggcg cagatacgct tccggatcga taccgttcag acggcaggtg
     3121 ccgatcagcc cgtacagcag tgcaccacgc tcgccgccgt gatcgctacc gaagaacacg
     3181 taatttttct ttccgagaca gactgcacga agcgctcttt ccgctgtgtt attgtccgcc
     3241 tccgccagac cgtcatcact gtaataacag agggcgtccc actgattcag tacatagctg
     3301 aatgcttcgc ccagtctgga ttttttcgac agcgtgccat tcttctccac catccattca
     3361 tgcagcgacg tcagtaacgc tttgcttcgc tgctgcctga ctgcaagaca ctctgactct
     3421 ggtaatcccc gtatttcatc ctcgatggcg tacagttcac tgattcgctt cagggcttct
     3481 tctgccgtcg cacgcaagta atccggggcc cgctatgccg cgctctatcg ggcgggacgg
     3541 ggctggcgct
//
LOCUS       DAEEGA010000210         3547 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEEGA010000210 DAEEGA010000000
VERSION     DAEEGA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.213"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="KLH46_004175"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="KLH46_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBE0380605.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="KLH46_004176"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="KLH46_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBE0380606.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="KLH46_004177"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="KLH46_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBE0380607.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          772 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctgg
//
LOCUS       DAEEGA010000211         3539 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEEGA010000211 DAEEGA010000000
VERSION     DAEEGA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.214"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..322
                     /locus_tag="KLH46_004178"
     CDS             <1..322
                     /locus_tag="KLH46_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBE0380608.1"
                     /translation="ETGISRASVGRLCIELVASGDVYNSGYGLFPSEQARKDWQSARK
                     KLSRVKVRKPVVVDPDLIWSLPDGEIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQ
                     EAIL"
     gene            380..736
                     /locus_tag="KLH46_004179"
     CDS             380..736
                     /locus_tag="KLH46_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380609.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            829..1047
                     /locus_tag="KLH46_004180"
     CDS             829..1047
                     /locus_tag="KLH46_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBE0380610.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            1049..1414
                     /locus_tag="KLH46_004181"
     CDS             1049..1414
                     /locus_tag="KLH46_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBE0380611.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            1411..2076
                     /locus_tag="KLH46_004182"
     CDS             1411..2076
                     /locus_tag="KLH46_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380612.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            2076..2441
                     /locus_tag="KLH46_004183"
     CDS             2076..2441
                     /locus_tag="KLH46_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBE0380613.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2896..3051
                     /gene="hokD"
                     /locus_tag="KLH46_004184"
     CDS             2896..3051
                     /gene="hokD"
                     /locus_tag="KLH46_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBE0380614.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT         1019 a          724 c          917 g          879 t
ORIGIN      
        1 tgaaacaggt atcagtcgtg cgtctgtcgg tcgcttatgc atagaactgg tcgcaagtgg
       61 tgatgtatat aattctggct acggcttatt cccgtctgaa caggctcgca aggactggca
      121 aagcgcccgc aaaaaactct cgagagtaaa ggtgaggaaa ccggttgttg ttgatccgga
      181 ccttatctgg tcattacctg acggagaaat acgccgctac gacaggcgcc tgaatataat
      241 ctgtcgcgag tgccggaaga gcgaagctat gcagcgtgta ctggctttct atcagggtaa
      301 ttttcaggag gcgatactgt gaatgaaatt agctatcagg cttcaattac cgctggcatt
      361 cgcatcaaag gagaggagca tggaaataaa accagaagat gagttaagca atatcgtttt
      421 atttccgata aaagaggatg accctcgtaa tcaggttaat tttctttata agccatcgga
      481 aagaccatat tgccatcacg cctctgtacg ggttgacgaa aaagagcgtc aggtccgctg
      541 taaaatctgc ggtgcggttg tggagccgtt tgactggatg ctctctgtgg caaaaagaga
      601 aaccagactg gcagatgatg taaggctatt gcgccaggag gaacaggaaa ggcggagaaa
      661 tatagaaaag ctgatacaga ttgagcgtaa cgcgaaagcg cggatacgca gggcgacaaa
      721 atccagaact gaataattaa atttagcact gttaaaaatt taatccttaa ccggagggat
      781 ttctgcaccc tcaaatcatc aggagaccgc ccgaaagggc ggtaatgaat ggttacatta
      841 tttagaaaaa aatatccgcg aaagagtaga acaacagaat tcctgtttct cattctgttt
      901 atcgtgttga tgacaccgat atccccgcaa atttttgtct ggataatcgg aaaaataatt
      961 gagccagtta ttgaattgta taacgacgtg gtatgggcgt cattcaacac actgcacaat
     1021 aaaattaatc cgtataagga aagctgatat ggcactgacg aaaaaacaac gtgcagaact
     1081 gcgcatgaag ttcggtggtc gctgcgctta ttgcggctgc gaacttggcg aaaagtggca
     1141 tgcagaccat gtaaaaccgg tcattcgttt tgatggaaat atgcttcacc aggaacgtga
     1201 cgatatatcc aacatggtac cagaatgcca cccatgcaat ctgcacaagc attgcagtag
     1261 cctggaagat tatcgacgaa ttatcagtga tggtcgtcgt gaattccttg cgtccgggaa
     1321 aggcaaagcg ctggttcgta tgggattggt tgaaatgaaa tctgacccgg ttgtgttctg
     1381 gtttgaaaaa tatcaagaag gggctacggc atgactacta ttaccaaaga gcgactgcaa
     1441 tggctggcta acatttctgg ccgcgatgat attgacgata tagacggcgg tgaaattcgt
     1501 gaactggcgc ttatcgctct ggcatcactg gaagcagagc ctgtagcgga atgcattgtt
     1561 gaagatgggg gcatgtgtat tgacgggttc ggtgagtatg tgggtcactc gctgcctgat
     1621 ggaatgcatg agctttatgc tgccccgcga atgcgacaac ctgatggatg gaaagccgta
     1681 gctgtagcgt ggaaggtgac gtttactcaa gttgaccagg aatctaatac gttcactgct
     1741 atatattttt acaaagcgga agttgagcgt tgggtacgac tgcataaagc atgtgatttt
     1801 cgggcagata taacaccgct ttacgcagcg ccggcagtgc cggttgcagt aaacgacgac
     1861 atggcttacg cattccatca cgcactgtca gattcatcgc ttggctctga tgaaatcgaa
     1921 gaaattaaaa ccggtttgcg tgctgccttt gccaacgtca ctatccaacc agagccgata
     1981 gtgccggatg aaatcgggcc aaacgatagt aatacgtttg attatgttga tggctggaac
     2041 gcctgccgcg ctgccatgct taagggagat aaataatgat taatcgaacc aaactggagc
     2101 acattctcga atatgccagg cagcagaaat gtattgggca actttgtaaa attccaccag
     2161 gagatatggt tgaaatcgtg gaaatggcca tgcgtaaggc tggcaactct ccggtaactc
     2221 tggctggctg gataagctgt agtgatgcag ttcctgctga atattgcgat gtgattcttc
     2281 tcgatgatct cgggaatgta ttccccggtt cctgggataa ggttttttgc cccactcgtg
     2341 gcgggaataa gatggctttt gtggacaaag acggcgttga agtagagagc tcaactcact
     2401 ggatgccgct accggaacca ccgcaggagg tgaatcaatg acctggcctg aggcattcac
     2461 cacggtagga attgcgatgg cggtggcgct ggtggtgtat tcgatttgcc gttggggtta
     2521 acaaacaaaa accccggatt gatggtccgg ggtttttgaa ggaaacaaaa cagaaacagc
     2581 aattgccgtt acctgttgtt accatggcaa gtaaacgtat ctcaggcgag cgcattgcgc
     2641 cgttctgacg cagataaatt agcctggata gaaggtgctg gcaataaaaa atagcgtttt
     2701 cttatcggta tcggtaagat tgctgcgggt gcttgaggct gtctgcctcg ggcatgccac
     2761 tgtaaggcag acagagaaaa gccccagtta acattacgcg tcctgcaaga cgcttaacat
     2821 taatctgagg cccaatctat gtctcacaaa tgtaggttag cctcttacgt gccgaaaggc
     2881 aaggagaagc aggctatgaa gcagcaaaag gcgatgttaa tcgccctgat cgtcatctgt
     2941 ttaaccgtca tagtgacggc actggtaacg aggaaagacc tctgcgaggt acgaatccga
     3001 accggccaga cggaggtcgc tgtcttcaca gcttacgaac ctgaggagta agagaccagg
     3061 cgggggagaa atccctcgcc acctctcatg tgtcaggcat cctcaacgca cccgcactaa
     3121 acccgcttcg gcgggttttt tgttgcgtgc tgaatacgca gggtgaaaaa taaccatata
     3181 tttgattata tacacaacaa aaaataaaag tcattgtact tgcacattaa acaatcaaat
     3241 atacggcgtg aaataaatat ttatcagatt aatatttttg tctctatgtg gatataaccg
     3301 tttgtactta taaacctggg ggcatcgtgg aaaaaataaa gaaactattt agtagcaaat
     3361 acgcagtcat acgtcgtgat gacctgtcag ttatagtcga aatggattac ttccctgaaa
     3421 tcccaaaatc aataatgtat cgtaatggtc gaaaggcaat ttttttaccg atgagggtaa
     3481 gtgacattat gggaaatgat aaactgctgg atgaattgcg agtcagagca tcctgttag
//
LOCUS       DAEEGA010000212         3422 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEEGA010000212 DAEEGA010000000
VERSION     DAEEGA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.215"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            174..1277
                     /gene="proB"
                     /locus_tag="KLH46_004185"
     CDS             174..1277
                     /gene="proB"
                     /locus_tag="KLH46_004185"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBE0380615.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1289..2542
                     /gene="proA"
                     /locus_tag="KLH46_004186"
     CDS             1289..2542
                     /gene="proA"
                     /locus_tag="KLH46_004186"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBE0380616.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2657..2732
                     /locus_tag="KLH46_004187"
     tRNA            2657..2732
                     /locus_tag="KLH46_004187"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2690..2692,aa:Thr,seq:cgt)
     gene            complement(2747..>3422)
                     /locus_tag="KLH46_004188"
     CDS             complement(2747..>3422)
                     /locus_tag="KLH46_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE0380617.1"
                     /translation="WKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVE
                     FALATGLRRSNIINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGN
                     HHRWVFVYKESCTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASW
                     LVQAGVPLSVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNK
                     EGTNDV"
BASE COUNT          803 a          865 c          928 g          826 t
ORIGIN      
        1 aaaatgtggt aatttattaa atctgtaata aaagcgtaaa caactgccgc tacgcttgct
       61 gatcccgcgc aacaaaacgc catgctttgc tcgcagatgg ttggcaaccg acgacagtcc
      121 tgctaaaacg ttcgtttgat atcatttttc ctaaaattga atggcagaga atcatgagtg
      181 acagccagac gctggtggta aaactcggca ccagtgtgct aacaggcgga tcgcgccgtc
      241 tgaaccgtgc ccatatcgtt gaacttgttc gccagtgcgc gcagttacat gccgccgggc
      301 atcggattgt tattgtgacg tcgggcgcga tcgccgccgg acgtgagcac ctgggttacc
      361 cggaactgcc agcgactatc gcctcgaaac aactgctggc ggcggtaggg cagagtcgac
      421 tgattcaact gtgggaacag ctgttttcga tttatggcat tcacgtcggg caaatgctgc
      481 tgacccgtgc tgatatggaa gaccgtgaac gcttcctgaa cgcccgcgac accctgcgtg
      541 cgttgctcga taacaatatc gttccggtaa tcaatgagaa cgatgctgtc gctacggcag
      601 agattaaggt cggcgataac gataaccttt ctgcgctggc ggcgattctg gcgggtgccg
      661 ataaactgtt gctgctgacc gatcaaaaag gtttgtatac cgctgatccg cgcagaaatc
      721 cgcaggcaga actgattaaa gatgtttacg gcattgatga cgcactgcgc gcgattgccg
      781 gtgacagcgt ttcaggcctc ggaactggcg gcatgagtac taaattgcag gccgctgacg
      841 tggcttgccg tgcgggtatc gacaccatta ttgccgcggg cagcaagccg ggcgttattg
      901 gtgatgtgat ggaaggcatt tccgtcggta cgctgttcca tgcccaggcg actccgcttg
      961 aaaaccgtaa acgctggatt ttcggtgcgc cgccggcggg tgaaatcacg gtagatgaag
     1021 gggcaactgc cgccattctg gaacgcggca gctccctgtt gccgaaaggc attaaaagcg
     1081 tgactggcaa tttctcgcgt ggtgaagtca tccgcatttg caacctcgaa ggtcgcgata
     1141 tcgcccacgg tgtcagtcgt tacaacagcg atgcattacg ccgtattgcc ggacaccact
     1201 cgcaagaaat tgatgcaata ctgggatatg aatacggccc ggttgccgtt caccgtgatg
     1261 acatgattac ccgttaagga gctggccgat gctggaacaa atgggcattg ccgcgaagca
     1321 agcctcgtat aaattagcgc aactctccag ccgcgaaaaa aatcgcgtgc tggaaaaaat
     1381 cgccgatgaa ctggaagcac aaagcgaaat catcctcaac gctaacgccc aggatgttgc
     1441 tgacgcgcga gccaatggcc ttagcgaagc gatgcttgac cgtctggcac tgacgcccgc
     1501 acggctgaaa ggcattgccg acgatgtacg tcaggtgtgc aacctcgccg atccggtggg
     1561 gcaggtaatc gatggcggcg tactggacag cggcctgcgt cttgagcgtc gtcgcgtacc
     1621 gctgggggtt attggcgtga tttatgaagc gcgcccgaac gtgacggttg atgtcgcttc
     1681 gctgtgcctg aaaaccggta atgcggtgat cctgcgtggt ggtaaagaaa cctgtcgcac
     1741 taacgcggca acggtggcgg tgattcagga cgccctgaaa tcctgtggct taccggcggg
     1801 tgccgtgcag gcgattgata atcctgaccg tgcgctggtc agtgaaatgc tgcgtatgga
     1861 taaatacatc gacatgctga tcccgcgtgg tggcgctggt ttgcataaac tgtgccgtga
     1921 acagtcgaca atcccggtga tcacaggtgg tataggcgta tgccatattt acgttgatga
     1981 aagtgcagag atcgctgaag cattaaaagt gatcgtcaac gcgaaaactc agcgtccgag
     2041 cacatgtaat acggttgaaa cgttgctggt gaataaaaac atcgccgata gcttcctgcc
     2101 cgcattaagc aaacaaatgg cggaaagcgg cgtgacatta cacgcagatg cagctgcgct
     2161 gacacagttg caggcaggcc ctgcgaaggt ggtggcggtt aaagccgaag agtatgacga
     2221 tgagtttctg tcattagatt tgaacgtcaa aatcgtcagc gatcttgacg atgccatcgc
     2281 ccatattcgt gaacacggca cacaacactc cgatgcgatc ctgacccgcg atatgcgcaa
     2341 cgcccagcgt tttgttaacg aagtggattc gtccgctgtt tacgttaacg cctctacgcg
     2401 ttttaccgac ggcggccagt ttggtctggg tgcggaagtg gcggtaagca cacaaaaact
     2461 ccacgcgcgt ggcccaatgg ggctggaagc actgaccact tacaagtgga tcggcattgg
     2521 tgattacacc attcgtgcgt aaataaaacc gggtgatgca aaagtagcca tttgattcac
     2581 aaggccattg acgcatcgcc cggttagttt taaccttgtc caccgtgatt cacgttcgtg
     2641 aacatgtcct ttcagggccg atatagctca gttggtagag cagcgcattc gtaatgcgaa
     2701 ggtcgtaggt tcgactccta ttatcggcac cattaaaatc aataagttac acatcattag
     2761 taccttcctt attttttgac tgggacaaat ttgggaccga tgggttcagg atcgagtcta
     2821 tttgccgtgc gtgttcggta aggtgattag gtgcaaggtg agcatatcga cgaaccattt
     2881 cgatagactc ccagcctccc atttcctgta acactgacaa cgggactccg gcttgaacca
     2941 gccaacttgc ccaggtgtgt ctcaagtcgt gaaatctgaa atcatcaata ccagcccgtc
     3001 tcagcgccgc tttccaggct gtgtttgcgt cataccgcat cttccttact gttggcgctt
     3061 tcgttccgtc tggtttggta cagctttcct tgtacacaaa tacccaacgg tgatgattcc
     3121 cgatttgttt tttcaatacg cgacatgcag tatcattcag cgcaacgcca attgcgcggt
     3181 ttgatttact ctcttccggg tttatccatg ccacccggcg ctgcatatct atttgttgcc
     3241 attcaaggtt gatgatgttc gagcgtctta agcctgttgc cagtgcaaat tcaacaacag
     3301 actttaatgg ctccggacat tcatcaatca gcctttgtgc ttcatggggc tccagccagc
     3361 ggatccgttt attctttggt tgaggcactt taataattgg tgccttatcc agcattttcc
     3421 at
//
LOCUS       DAEEGA010000213         3405 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEEGA010000213 DAEEGA010000000
VERSION     DAEEGA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.216"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..693)
                     /gene="hypT"
                     /locus_tag="KLH46_004189"
                     /gene_synonym="qseD"
     CDS             complement(<1..693)
                     /gene="hypT"
                     /locus_tag="KLH46_004189"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBE0380618.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSEL"
     gene            1038..1757
                     /locus_tag="KLH46_004190"
     CDS             1038..1757
                     /locus_tag="KLH46_004190"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBE0380619.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            1823..3121
                     /locus_tag="KLH46_004191"
     CDS             1823..3121
                     /locus_tag="KLH46_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HBE0380620.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT          839 a          755 c          813 g          998 t
ORIGIN      
        1 taactcgctc atcgaagaga taaatatcgt gcgcccggta atacctccaa cctctgccag
       61 atgacgattg accaacctgc ccatataaga tgtggcggta tagttgagca aaggaacctg
      121 gggctgagtg atatcgaata cagggtgccc ctgtgcgtcg gcggcacata ccgggtacaa
      181 ctcagattca aacagtttca ggcagcaaaa aggcgattgc ataagatctt catcgcgaaa
      241 cgaaataata aagtcactct tgccttcgcg taacgtattt acggcctgga caacatcaat
      301 ggcttcaaca tgatagacaa attctccgcc ataggcggta agtgaatgca ccagtttggg
      361 taatacgctc agggatagtg agtgcgcggc tgcaatttta atgttgggaa cgccgagtaa
      421 actctggccg tttaactccc ccaaattgca ttcaagctgc tgtaataagc tgcgagtctg
      481 ggaatgaaag agcttgccct cttcggttaa ttgtaaaggg gtggtcgtgc gatcgaataa
      541 aacaacgccg atcgctgcct ccagcgcctt aattcgtcgg ctgaatgcgg gttgcgaaag
      601 attacgctct tttgccgcct gagaaaaatg gcgacaggct tccagtgtca ggaaatcgta
      661 taaccatttt gtctcgatat tcttcattac catggcacct gtattttcaa tccaaagaca
      721 taataaaaca tcccggcaat aaaaataatg ttctcaatta gcacaacctg gcctttatca
      781 ttcgttttgt gcataacccc ttcatttccg gtattgacgt tgatatcaca tttcgccaaa
      841 aaaacgaaat ttttccgtca gataaccaga atacatcatc actatggtga ataagcggaa
      901 tgttccatag ctgcatcatg cgtgtcttgc atcgccttgt acaaccttgc atttctcatc
      961 gcccatttcg actgttaaaa aagagagcag cagttattta ccgcgtattg tcattaacgt
     1021 tttgagaggc aggtattgtg aaaggtttaa ttggtgtact cggtggaatg gggccggcag
     1081 ctaccgtcga cctgtttaat aaatttgtaa cttttaccgc tgcacaatgt gatcaggaac
     1141 atattccttt aattatttct tcgatcccgg atattcctga ccgtaccgat gcattaatgc
     1201 atcatgggcg ttcaccatta cccgttatgc gtgattatat gcataagctg gaagatgccg
     1261 gggcagaatg tattgttatt ccctgtaata ccgcgcatta ctggttcaaa gaattaaaag
     1321 atgcttgtca tattgatata ttaagtatcg tagaaactac gatcaatgaa gttcgcgcct
     1381 gtggtaaaac ccgaattggc ttgttagcca ccaatgccac gctatatatg ggtttatacc
     1441 aaaaaggaat tgagtcgctg ggatttacct gtgtcagccc cgatgccgac ggtcagaaaa
     1501 aagtgatgga aagtatttat tccttgaaag ctggcaatat agcgcacgca caaaagttga
     1561 tgaatgagca ggctgaaata ttattttccc gcggtgcaga aatccttgtt cttggttgta
     1621 cagaaattcc ggttattctg gcgcaagccg ttaaagagca gccttcccgc tatattgact
     1681 caacggcgtc actcgttcgt gccggaataa aatggtacga aaatcgtgtc ggtaaacatt
     1741 atcttttaac ccaataatca tttaaatcgc agccagaaag ttattcgctt ttaactgaat
     1801 tatatttata acggagaaca ttatggtttg gctggaaatt atcgtagtac ttggtgcaat
     1861 attttttggt attcgccagg ggggaatcgg tattggttta tgtggcgggc ttgggcttgc
     1921 cattctgact ctgggatttg gtctgcctat ggggtcgccg ccagtcgacg tgatcctgat
     1981 tattatgacc gttgttgtgg cggcctcagc cttacaggcc gcaggcggaa tggattatct
     2041 ggtacgcgtc gctggtaatt ttatgcggcg taacccgaaa tatatcaaca taatcgcgcc
     2101 gattatcacc tggtctatga ccattatggc cggtactggg tttattgttt tttccacgct
     2161 gccggtgatt gctgaagtgg cgaaagagtc cggtatccgt ccttcccgca cgcttgccgg
     2221 ttcggttgtc gcttcgcagg ttgcgatttc cggttcgccg atcagcgccg caatggcggc
     2281 tatgctcact attatggagg taaacggtgt cagttttatt caagtgatgt cggtatgtct
     2341 tcccacctct tttgctgccg cgatggtcgc cgcttttatt gcttcccgac agggatgtga
     2401 gttgcaggat gacgaggtct accttgagcg cctgcaaaaa ggactggtgc aaaaatacga
     2461 gaataatagc agcatcaagc caggtgcgac gctctccgtc ggattgttcc tgctggcgac
     2521 ggtcgctatt gttattcttg ctgccttccc ccaactgcgt ccgggttttg atatcggtaa
     2581 accgatggaa acacgcgata tcattattat ctgtatgctc tctgccgcct gcctgatggt
     2641 ggtgctgtgt aagacggcga ccgatgccat tattctcacc tccacattcc gtgcggggat
     2701 gagttcgctg gcggtaattc tcggcattgt aaccttaggc accactttta ttgatgcgca
     2761 tttaactgag attaaagata ttgctggtga tattttgcag gcgtatccga tgttgttggc
     2821 gctggttctt tttggcactt gcgcgctgct ctactcgcaa ggtgcgacca cgccgttaat
     2881 tatcccgctt gccgtcgcgc ttgacgtgcc cacctgggca attctggcgt cctatgtggc
     2941 cgtcaccgga gtatttgtac tgccaacgta tccaacatca ctggctgcca tggaatttga
     3001 tactaccggc acaactcgtg taggcaaata tgtgctgaat catccattta tgctgcctgg
     3061 gttgggggga attattgctg gagtggcatt aggctttgtg attgcgccaa tgattattta
     3121 gcactatcaa acatcagtat cgatggttta tacacaataa agacagatat ggcggaatat
     3181 agtctgttgt aagcgccaac aaagaatagg ggaattccgc catgtctgaa catcttcaca
     3241 atatggtatt aagtgttgtc gtttaatgat ttatttcttg gttatgttgt gtattagctg
     3301 catctatcgc tttttgatac ctgaggtgta ctggcaatag cggacactac catttgttct
     3361 ttttttaagc agtcatctga tgatattttt ccctgaaggc tgccg
//
LOCUS       DAEEGA010000214         3309 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEEGA010000214 DAEEGA010000000
VERSION     DAEEGA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.218"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            86..1504
                     /locus_tag="KLH46_004192"
     CDS             86..1504
                     /locus_tag="KLH46_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBE0380621.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            1731..3182
                     /gene="uxaB"
                     /locus_tag="KLH46_004193"
     CDS             1731..3182
                     /gene="uxaB"
                     /locus_tag="KLH46_004193"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBE0380622.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT          769 a          765 c          862 g          913 t
ORIGIN      
        1 ctgttagcga acatgctatc ctttatgaga atttacttat cacgttgaga taattgccac
       61 ccgcaattgt atggacagat aattaatgca tgtacacccc atttcaactt tccgtttgtt
      121 ccaggaaggt catctgctac gtaatagcat cgctattttt gcgctaacca cgctgttcta
      181 ttttattggt gcagagttac ggctggttca cgaactttct cttttctggc cgctgaatgg
      241 cgtaatggcg ggggtgtttg cccgctatgt ctggcttaat cgactgcatt actatgcgat
      301 cagttatgtg gcgatgctgg tttatgatgc cataaccacc gaatggggac tggtttcact
      361 ggttatcaat ttctccaata tgatgtttat tgttaccgtc gccttactgg tcgtgcggga
      421 taagcgtctg gggaaaaata agtatgagcc agtaagtgct ttacggctat ttaattactg
      481 tctgattgcc gcattattat gcgctattgt cggggcgatt ggttcggtca gtattgatag
      541 tctggatttc tggcctttgc ttgcggactg gttcagtgag caattctcaa cgggcgtgtt
      601 gatcgtgcct tgtatgctga cgttggctat tcctggagtg ctgccgcgct ttaaagcaga
      661 acagatgatg cccgccatcg cgcttattgt gtcggttatt gcctcggtag tcattggcgg
      721 agcggggagt ctggcgttcc cgctgcctgc attaatctgg tgtgcagtgc gctatacgcc
      781 gcaggtaaca tgtctgttga cctttgtcac cggtgcggtg gaaatcgtac tggtggcaaa
      841 ttcggtgatt gatatctcgg tcggttcgcc gttctccatt ccggaaatgt tctccgcacg
      901 tctcggtatt gccacgatgg cgatatgccc aattatggtt tcttttagcg tggcagcgat
      961 caatttgcta atgaagcaag ttgcgctgcg agccgacttt gattttctga ctcaggttta
     1021 ctcacggtcc ggtctttatg aggcgctgaa aagtccatcg ctgaaacaga cacaacatct
     1081 gacggtcatg ctgcttgata tcgactattt caaaagcatt aacgataact atggacatga
     1141 atgtggcgat aaagtgttaa gcgtgtttgc ccggcatatt cagaagattg tcggtgataa
     1201 ggggctggtg gcgcgcatgg gcggcgaaga atttgctgtt gcagtgccgt cggtgaatcc
     1261 tgtcgatggt ctgctaatgg cggaaaaaat ccgtaaaggc gttgaactgc aaccattcac
     1321 ctggcaacaa aaaacgcttt atctcacggt aagtattggc gtcggtagtg gtcgcgcatc
     1381 gtaccgaacg ctgaccgatg attttaataa attgatggtc gaagccgata cgtgtctgta
     1441 tcgctcgaag aaagatgggc gcaaccgtac cagcactatg cgttacggtg aggaagttgt
     1501 ctgacatctt tattcattcc gcgcgatttg aatgcaatgt gttctaccac tttatgatcc
     1561 aggaaaaggc gtatttcctc gattaaacca tgatccgcgc cacacttttc tcataaaact
     1621 gctcattaat catcaggagt tgatcaaaac aggggttcgg cgtattaaag tttgctaacc
     1681 tgtcgcacca attcaaacag gttgtatgac taatcagaag ggaacccatt gtgaaaacac
     1741 taaatcgtcg cgattttccc ggtgcacagt atccagaacg tatcattcag tttggtgaag
     1801 gtaacttcct gcgcgccttt gttgactggc aaatcgatct cctgaatgag cacaccgatc
     1861 tgaattctgg cgtggtcgtt gttcgtccga ttgaaacttc attcccgccg tcactgagca
     1921 cgcaggatgg tctgtacacc accattatcc gtggcctgaa tgagaagggg gaagcggtca
     1981 gcgacgcgcg tctgattcgc tcggttaatc gtgaaatcag cgtctacagt gaatacgatg
     2041 aattcctgaa actggcgcac aacccggaaa tgcgctttgt tttctctaac accaccgaag
     2101 cgggtatcag ctaccatgcg ggcgataaat tcgatgacgc gccagcggta agctatccgg
     2161 caaaactgac ccgtctgctg ttcgaacgct ttagccattt caacggtgcg ctggataaag
     2221 gttggatcat cattccgtgt gagttgattg actacaatgg cgacgcgttg cgtgaactgg
     2281 tgctgcgcta tgcgcaagag tgggcactgc cagaagcatt tattcaatgg ctggatcagg
     2341 ctaacagctt ctgttctacg ctggtggacc gtatcgttac cggttatccg cgcgatgaag
     2401 tggctaaact ggaagaagaa ctgggttatc acgatggttt cctcgacacc gctgaacact
     2461 tttacctgtt tgtgattcag gggccgaaat ccttagcgac tgagctgcgc ctggacaaat
     2521 atccgctcaa cgtgctgatt gttgacgata ttaaaccgta taaagaacgc aaagtggcaa
     2581 tcctcaacgg tgcgcacacc gcgctggtgc cggtggcgtt ccaggcaggg ctggataccg
     2641 taggtgaagc gatgaacgat gctgaaattt gcgcattcgt tgaaaaagcc atctacgaag
     2701 aaattattcc ggtactggat ttgcctcgtg atgaactgga atctttcgcc agtgcagtca
     2761 ccgggcgttt ccgtaacccg tacattaagc atcagctgct gtctatcgcg ctcaacggta
     2821 tgaccaagtt ccgcacccgc atcctgccgc agctgctggc agggcagaag gcaaatggca
     2881 cacttccggc gcgccttact ttcgcattag cggcattgat tgcgttctat cgtggtgagc
     2941 gtaacggaga aacatatccg gtacaggacg atgcacactg gctggaacgt tatcagcaac
     3001 tctggagcca gcatcgtgac cgtgttatcg gtacccagga actggtagcg attgtgttgg
     3061 cagaaaaaga ccactgggag caggacctga cgcaagtgcc tggtctggtc gagcaggttg
     3121 ctaatgacct ggatgcgatt cttgaaaaag ggatgcgcga agctgtacgt ccgttgtgct
     3181 aataacaccg ttacttctga acccggtttc gaccgggttt cttatttcac tatgttagtt
     3241 acaacatcct atatcccttt atcattaaca ggtgtcacat gcagcgcctg ttaatgttgt
     3301 aagcttgac
//
LOCUS       DAEEGA010000215         3180 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEEGA010000215 DAEEGA010000000
VERSION     DAEEGA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.219"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            419..1528
                     /gene="fepE"
                     /locus_tag="KLH46_004194"
     CDS             419..1528
                     /gene="fepE"
                     /locus_tag="KLH46_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBE0380623.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2553..2747)
                     /locus_tag="KLH46_004195"
                     /pseudo
     CDS             complement(2553..2747)
                     /locus_tag="KLH46_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          802 a          658 c          775 g          945 t
ORIGIN      
        1 aaaaaaaaaa catcatctta tattaggggt gctgctagcg gcgcggtgtg tttttttaca
       61 ggacgccctg ggggcgctgc taggggtgtc tgttcagata tgtagcaatg agtcagggat
      121 tcacgaactg aaaatgtccg aaaatgtgtc tcatgggcac tgtatgcgcc caaaccgatc
      181 gtcattcact ttcatgcata gctatgcagt gagctgaaag cgatcctgac gcatttttcc
      241 ggtttacccc ggggaaaaca tctctttttg cggtgtttgc ggcagaatcg cgttcagcgc
      301 gttttagcgg tgcgcgcaat aaaatgttat gttaaatgtc tgctgaatgg tttttaggtt
      361 ttttcctaac atatgataga ataaaaaaac catatgttaa tatattgtgt ttttttcaat
      421 gaaaatctca aaggtctctg acgaacattt ttctaattac aacatgttgc cgaatggtaa
      481 tgaagaaatt gacctttttg gcttgattag cgttttatgg gcagcaaaaa taaaaattgt
      541 tggggtgatt ctattttttg cttgtattgg ctttcttgtt tcttttgttt tgccacaaaa
      601 atggactagt tctgctgtgg ttactcctgc tgagtctgtt caatggagtg aattaaataa
      661 ggagctttcc aagcttcatg ttctgggagt gggttttgat atcaatcgtg atagcgcatt
      721 tgctctgttt attaagaaat ttcagtccgt taattcgttg aatgagtata tgagatcatc
      781 tccttatgtt atggagatta ttaaaaagaa taacattagc gctctcgaac tgcatcgtgc
      841 aattgttgag ttgagtaaaa atatgaagtc gattgatgat aatgccagta agaaaaacga
      901 caagtcatca ttgtatgtat catggactct gagttttact gctccaacaa gtaaagaagc
      961 tcacgatgtg ttgtctgggt atattaatta tgtttcttcc cttgttgtaa gggatttgat
     1021 ggaagatata agaaataaac tagaagttaa aactaatgtt gaaaaagaaa ttcttgcact
     1081 ggatgagata aaaattagaa accagctgaa tgcagatatt cgacgcctca attattcact
     1141 ggaggttgct aatgcggctg gaataaaaaa acctgtatac agcaatggtc agattatgaa
     1201 ggatgaccca gattttcctg tggctctcgg ttctgatggt atagcaacta aattgaacat
     1261 caaaaaatca atcaaggatg tttcggaatt gagtggggag ttgcgaaatc gtcaatatgt
     1321 tgtgaatcaa ttggttgtgg cgaaagtggg ggatgttgat tttatgccat ttcagtatca
     1381 attgagccca acgttaccgg tcagaaagga tgggccaagt aatgccataa ttgtaattct
     1441 ttcttctgtc attggaggga tgcttgcatg tggctatata cttttacgtc atgccatgga
     1501 ggccagaaag atgagtgtga ttgagtaaaa gttgtgctga tgcatcattt tataagcaac
     1561 ttttataaaa agttcaaagg atcttcttga gatcattccc ttacagccgt agtcttctac
     1621 tctagaaaac gaaaaaacca cctggggagg tggtttttcg aaggttcagt aagttgggga
     1681 acttctgaac cgtggtaaca gtcttgtgcg agacatgtca ccaaatctgt ccttctagtg
     1741 tagcctcagt aaggctgcca cttcaagaac tctcaataca tctctcgcac atcctgttta
     1801 ccagtggccg ctgccaatgg cgttaagtcg tgtcttaccg ggttggactc aagacgatag
     1861 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1921 gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacgaac gcggccataa
     1981 cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccacc
     2041 agggggaaac gcctggtatc tttaagtcct gtcgggtttc gccacctctg acttgagcgt
     2101 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccac cggcgcggcc
     2161 ttttttcggg gcctctcttt tgctcacatg ttttttctgg tgttatcccc tgattctgtg
     2221 gataaccgta tatccgtctt tgcgtgagca gacaccgctc gccgcagccg aacgaccgag
     2281 cgcagcgagt cagtgagcga ggaagcggaa gagcgccgga agctgcattt tctccttacg
     2341 tatctgtgca gcatttcaca cctcatatgt cgctgttctc tcaccatctg ctctgccgcc
     2401 tcataattaa gccagtatac actccgttat cgctacgtga ctgggtcatg gctgcgcccc
     2461 gacacccgct aaaaccccct gacgcgccct gacgggcttg tctgctgacg aaaacgccca
     2521 cgggaaagcc gtgaccgcct ccgtcagctt cgttatgttc ctgtttcact ttcggcctca
     2581 aagcgaatct ggatgctgtt ctggagttct tctgcgagtt cgtgcagtcg tttgcacatg
     2641 gccgcctgtt cgtcggcatc cagagcatcc agtttttcaa gcagtgtcag gctctggctt
     2701 ttgatacatc gcgccatatt cagcgcagtt tgttgttatt tgttcacctt ccgttgttct
     2761 ccgtcagttc cgggtgtcgg gggggggacc ctgagcaggt ggtaattgta agacgttgcg
     2821 ggcgatggta ttacaattgc acatcctgtc ctgtttcttt aggacgtatc atggcacagc
     2881 atatcaactg ataaataatt cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg
     2941 cgtcgagtgt ttcttaatca ataattccgc tgatactccg gaaaaacgcg agtctgttct
     3001 catgcctgaa atgcccacac cccacgcaaa aacaagtttt tgctgatttt tctttataaa
     3061 tagagagtta tgacaaatta gttcttcttg ctctctttgt gatatttaaa aaagcggtgt
     3121 cggcgcggtg ttgtagctgc gccaacaccg ctttttaggg gtggtactga ctattttcat
//
LOCUS       DAEEGA010000216         3165 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEEGA010000216 DAEEGA010000000
VERSION     DAEEGA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.220"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(85..357)
                     /locus_tag="KLH46_004196"
                     /pseudo
     CDS             complement(85..357)
                     /locus_tag="KLH46_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            complement(451..1013)
                     /locus_tag="KLH46_004197"
                     /pseudo
     CDS             complement(451..1013)
                     /locus_tag="KLH46_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1217..1423)
                     /locus_tag="KLH46_004198"
     CDS             complement(1217..1423)
                     /locus_tag="KLH46_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBE0380624.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1530..2093)
                     /locus_tag="KLH46_004199"
     CDS             complement(1530..2093)
                     /locus_tag="KLH46_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HBE0380625.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
BASE COUNT         1000 a          698 c          614 g          853 t
ORIGIN      
        1 gcttattatg cttccatcgg aaacgatgat ctggcagcct gagttcacag ataaaacact
       61 ctccaggaaa cccggggcgg ttcacttaca ccagacatcg ttattaagta gcagcaccat
      121 aagataagga gtggcatcgt tagccacagc ttccctactc cagaggtaat ataaaggggt
      181 gggctcgact gatttatctt tacgtcgctt acactgcaaa tgttcagaaa ttagtctatg
      241 cagctcacca gtaaaatcag caatcagaga aggaattgcc ttattcatac cagggcaagg
      301 tattaattta aactgtaata atttaatttt aggatgtgta gcagcagccc gatacagagt
      361 tgcaaggaca cacttttgcc agagggcgtt acaggaaagc ttaatgtttg attctgtata
      421 cataataaat caccttatag ttacaatagg tcaaaaaccg ctgtagccaa agttacgctg
      481 gcctgatgct ttagtgtcga gcttcgtcaa ataatccaga cgtaccaata agcgctggta
      541 ctgctctggg aaattaggat cgttaatatc caggatgtaa cgtccattag cagggaaatg
      601 aactaatccc tggtgatctt ccagttccaa ccctaaggca ctgtaccagg ctccggtaat
      661 taaccctctc agggtgtcag agcgctcata gtttcccaga tggtaatagg cgtctttgtt
      721 aaaaaaaggc agacgtgata atgacattta aaggactgcg tatattcttt cgcccagata
      781 atacaaaccg acgttgcgta ggtgcgtttg ccttgttgtt ttttacggtg ttgatctgca
      841 ttaattttcg cattcagcga gttacggaaa cgagatatct ctccggggat caggttaggg
      901 aaacaggtaa tgatatcccc gttgtcaatc acaggagggt aatggagatc cccccgtatt
      961 gctgttattc tggaagtgtt tttaatattc gtattgatag ttctcagaga tatatcacac
     1021 tctagatatt ttaattaagt atcagttcga tatcggcata ttgtacacta cctcaggtag
     1081 atgatgagat cagatacagg gatcaactga cggtgagcgc agtccccaca ttttatccag
     1141 ggcttactgc atattcccaa cagccattcg ttagccaggt agacgaatac cctgatttgc
     1201 cgctggtttt gctttcctat cgaagggccc atacatcatc acgcccacga aacaaccgac
     1261 gaaataacgc aactttctca tccgtggata atacgaaaac gcactgcaca gggctctgcg
     1321 gtttacgtcg ccattcaatc ccatgagctt caagtaatga gaccagccgg gtattttctg
     1381 cccgcagtac tgctagttca tcgctgtcag atttataaac catatgcgtt atcacttgta
     1441 cctgtctccc ggcggcacca cgactgaaat tcgtgtgaag gcaaatccac gcgataactt
     1501 acgatagttc ggacataagc ccccataatt cagttatgtc gtggtcgacc ccggttactt
     1561 ttttcgggaa gcctgctacg gcccagaata ataccgtgtt ctttctgatc ctgtttagcc
     1621 agatagctaa gggcgtaacg taagtcgtct accgcagatt tatcactata gtgaatcacg
     1681 tgatcgatac gcaccgggta tttgtcttta gcccggcaca gatgaaaata accctcccct
     1741 tccgtgatcc gcctccagat atctcccagt tgcctggata tctgataaga gttcttgtga
     1801 cgttgtccat ccaggtagcc gataaaatga atatgaagcc ctttgtctgg tgtatattcc
     1861 attacccagt aatatcccgc caacatcgtc tgggtttcgc taagtaaacg gtatatttcc
     1921 atacacatac tgtgcttaca ggaatgcccg aaactgggcg tgtctttcct gtaagcaaaa
     1981 tcaattctga aaggtaacag tttagaaaaa cgttgaaaca taccatccat gtgttcattc
     2041 acgtctttca gaatcatgaa atccattttg taattgggat tggcattata cattttatag
     2101 tccttacttt ataaaactca caggaagaga gaattacgta aatactgaga gtagagaaac
     2161 gccgtaccac ggcattaata aacgaagtaa accagcagca cagacaacct gataaaactg
     2221 ttttgtatta ccgcattact gtgatgaggt aatacttcag aatacagtga gactctctta
     2281 aactgagaac cagtacgaat tacaatggaa aaacacacct catagaattg agtttcagga
     2341 agcaccacta aattctttac aggaagtaat ctgacattca caaaactcag tatcggctag
     2401 ctacaggcaa catcgctatt aatattgtcg aaccattaag taaagccgag agagaccacg
     2461 ggtatttaat cattagcata aaagaataat ggtatacaga acatacccac acaccataag
     2521 aagattgttc catacagaaa agatcatatt tacctatacc tataaactgt aacggagtca
     2581 taacacgctc taatagtgtt ccgatacgta accagtgtta agtacgtaca ggacaacatt
     2641 acacacataa ttcaccatac agcttccccc atatgcttca ctgcgtagtg tatggagata
     2701 tatgcattac acacatacag aatacttagc ataaagagga gtgacattac cacgtaaaga
     2761 gataagttat acaacaaaca tacaaacaat acggcacaga cacacaatac cataactcca
     2821 actaataata ctaattaacc ttaattattt atggtatatg aaataaatgt gattctcccg
     2881 gaactaacta acctaaccag acagatgatc actgtataaa aacttcagtt aatactcatt
     2941 tgttttgtcg actcagtata taatgagtga ccagtaatca ggataatcac acttacataa
     3001 cctgtaaaaa agaacaagaa gaataagcca ctaacataat gcaaaatggc atctgaatcg
     3061 atcgcagttt ggcattcgaa tcgatcatga tttggcccaa tccgtgatcg aatagagcgg
     3121 aatacgcaac ataattcacc agacaagagc ttacaccgaa ttaca
//
LOCUS       DAEEGA010000217         3119 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEEGA010000217 DAEEGA010000000
VERSION     DAEEGA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.221"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            102..3005
                     /locus_tag="KLH46_004200"
     rRNA            102..3005
                     /locus_tag="KLH46_004200"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          834 a          676 c          967 g          642 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgagtcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttattacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1321 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1381 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1441 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1501 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1561 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg
     1621 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1681 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1741 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1801 aaggcacgct gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata
     1861 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1921 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc
     1981 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     2041 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     2101 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     2161 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     2221 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     2281 tttaatgttt gatgttctaa cgtggacccg tgatccgggt tgcggacagt gtctggtggg
     2341 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2401 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2461 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2521 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2581 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2641 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2701 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2761 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2821 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2881 gagttctccc tgacccttta agggtcctga aggaacgttg aagacgacga cgttgatagg
     2941 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     3001 accttacaac gccgaagatg ttttggcgga ttgagagaag attttcagcc tgatacagat
     3061 taaatcagaa cgcagaagcg gtctgataaa acagaattag cctggcggcc gtagcgcgg
//
LOCUS       DAEEGA010000218         3112 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEEGA010000218 DAEEGA010000000
VERSION     DAEEGA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.222"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="KLH46_004201"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="KLH46_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HBE0380626.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="KLH46_004202"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="KLH46_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="KLH46_004203"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="KLH46_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HBE0380627.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="KLH46_004204"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="KLH46_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HBE0380628.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="KLH46_004205"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="KLH46_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          772 a          754 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcaccgctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAEEGA010000219         3067 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEEGA010000219 DAEEGA010000000
VERSION     DAEEGA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.223"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            542..1708
                     /gene="nhaA"
                     /locus_tag="KLH46_004206"
     CDS             542..1708
                     /gene="nhaA"
                     /locus_tag="KLH46_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBE0380629.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1774..2673
                     /gene="nhaR"
                     /locus_tag="KLH46_004207"
     CDS             1774..2673
                     /gene="nhaR"
                     /locus_tag="KLH46_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBE0380630.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2711..>3067)
                     /locus_tag="KLH46_004208"
     CDS             complement(2711..>3067)
                     /locus_tag="KLH46_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBE0380631.1"
                     /translation="NYQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILK
                     NELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNV
                     SSGQIMSRVKIVVMYN"
BASE COUNT          721 a          677 c          753 g          916 t
ORIGIN      
        1 atgtgttcag catataggag gcctcgggtt gatggtaaaa tatcactcgg ggcttttctc
       61 tatctgccgt tcagctaatg cctgagacag acagcctcaa gcacccgccg ctattatatc
      121 gctctcttta acccattttg ttttatcgat tctaatcctg aagacgcctc gcatttttgt
      181 ggcgtaattt tttaatgatt taattattta actttaattt atctcttcat cgcaattatt
      241 gacgacaagc tggattattt ttgaaatatt ggcctaacaa gcatcgccga ctgacaacaa
      301 attaattatt acttttccta attaatccct caggaatcct caccttaagc tatgattatc
      361 tgggcttagg gtcactcgtg agcgcttaca gccgtcaaaa atgcatctca ccgctgatgg
      421 cgcaaattct tcaatggctc gtaaaaaacg aattattcct acactataat ctgattttaa
      481 cgatgattcg tgcggggtaa aatagtaaaa acgatctatt cacctgaaag agaaataaaa
      541 agtgaaacat ctgcatcgat tctttagcag tgatgcctcg ggaggcatta ttcttatcat
      601 tgccgctatc ctggcgatga ttatggccaa cagcggcgca accagtggat ggtatcacga
      661 ctttctggag acgccggttc agctccgggt tggttcactc gaaatcaaca aaaacatgct
      721 gttatggata aatgacgcgc tgatggcggt atttttcctg ttagtcggtc tggaagttaa
      781 acgtgaactg atgcaaggat cgctagccag cttacgccag gccgcatttc cagttatcgc
      841 cgctattggt gggatgattg tgccggcatt actctatctg gcttttaact atgccgatcc
      901 gattacccgc gaagggtggg cgatcccggc ggctactgac attgcttttg cacttggtgt
      961 actggcgctg ttgggaagtc gtgttccgtt agtgctgaag atctttttga tggctctggc
     1021 tattatcgac gatcttgggg ccatcattat catcgcattg ttctacacta atgacttatc
     1081 gatggcctct cttggcgtcg cggctgtagc aattgcggta ctcgcggtat tgaatctgtg
     1141 tggtgtacgc cgcacgggcg tctatattct tgttggcgtg gtgttgtgga ctgcggtgtt
     1201 gaaatcgggg gttcacgcaa ctctggcggg ggtaattgtc ggcttcttta ttcctttgaa
     1261 agagaagcat gggcgttctc cagcgaagcg actggagcat gtgttgcacc cgtgggtggc
     1321 gtatctgatt ttgccgctgt ttgcatttgc taatgctggc gtttcactgc aaggcgtcac
     1381 gctggatggc ttgacctcca ttctgccatt ggggatcatc gctggcttgc tgattggcaa
     1441 accgctgggg attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg cgcatctgcc
     1501 tgagggaacg acttatcagc aaattatggt ggtggggatc ctgtgcggta tcggttttac
     1561 tatgtctatc tttattgcca gcctggcctt tggtagcgta gatccagaac tgattaactg
     1621 ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg gtaattggat acagctggtt
     1681 acgcgttcgt ttgcgtccat cagtttgaca ggacggttta ccggggagcc ataaacggct
     1741 cccttttcat tgttatcagg gagagaaatg agcatgtctc atatcaatta caaccacttg
     1801 tattacttct ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga ggcgctttat
     1861 ttaactccac aaaccattac cggacagatt cgagcgctgg aagagcgcct gcaaggcaaa
     1921 ttatttaaac gcaagggacg tggtctcgaa cccagcgagc tgggagaact ggtctatcgc
     1981 tatgccgata aaatgttcac cttaagccag gaaatgctgg atattgtgaa ctatcgcaaa
     2041 gaatccaatt tattgtttga cgttggcgtg gctgatgcac tttccaaacg cctggtcagt
     2101 agcgtactta acgccgcagt ggtagaaggc gagcccattc atcttcgctg cttcgaatcc
     2161 acccacgaaa tgctgctgga gcaattaagt cagcataaac tggatatgat catttctgac
     2221 tgtccgatag actctacgca gcaggaaggc ctgttctccg tgagaattgg cgaatgtggc
     2281 gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg tctggaagaa
     2341 cggcgacttt tgattcctgg gcgacgttca atgttagggc gcaaattgct taactggttt
     2401 aactcccagg gattaaacgt agaaatcctc ggcgagtttg atgatgccgc tttgatgaaa
     2461 gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc atatgacttt
     2521 tatgccgata aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga agagtaccat
     2581 gctatttttg ctgagcggat gattcagcac ccggcggtac agcgaatctg caatacggat
     2641 tattctgcgc tttttagtcc agcggtgcgt taatcggcag ctcccccaaa gttaaggtgg
     2701 gggagataga ttagttgtac attaccacga ttttgactcg gctcattatt tgcccgcttg
     2761 agacattgtt tccatatgta cgcgggcgaa taaatagagg aatctgatta cttccttcat
     2821 ggggatgctg aaaagagtag taattgcttt taccatactg gatttcattc gtaccatcat
     2881 tggagagttg aaagcctatt tgacccgcac cgtcctcact gttttctaac tcatttttta
     2941 atatgctggt tcctgactcc tgtatcgtca ctggttcaaa gtaatattca acgtcactgg
     3001 gttcaaaaac taattcacct tgctcgccct ggatgcagtc aaatcttata tgatgaaact
     3061 gataatt
//
LOCUS       DAEEGA010000220         3066 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEEGA010000220 DAEEGA010000000
VERSION     DAEEGA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.224"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..163
                     /locus_tag="KLH46_004209"
     CDS             <1..163
                     /locus_tag="KLH46_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0380632.1"
                     /translation="PKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACNG
                     LSDNRCLEI"
     gene            176..958
                     /gene="lsrF"
                     /locus_tag="KLH46_004210"
     CDS             176..958
                     /gene="lsrF"
                     /locus_tag="KLH46_004210"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HBE0380633.1"
                     /translation="MDWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINI
                     APLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRL
                     NSCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLAT
                     RIAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASG
                     VDMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            982..1272
                     /gene="lsrG"
                     /locus_tag="KLH46_004211"
     CDS             982..1272
                     /gene="lsrG"
                     /locus_tag="KLH46_004211"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HBE0380634.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1311..2069
                     /gene="tam"
                     /locus_tag="KLH46_004212"
     CDS             1311..2069
                     /gene="tam"
                     /locus_tag="KLH46_004212"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HBE0380635.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(2073..2987)
                     /locus_tag="KLH46_004213"
     CDS             complement(2073..2987)
                     /locus_tag="KLH46_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HBE0380636.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEEPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          807 a          707 c          825 g          727 t
ORIGIN      
        1 gcccaaacca gacgggttaa cggcagcaaa gaaccttgca gaggcgttcg agcattataa
       61 cgaatggcat ccgcatagtg cgctgggtta tcgctcgcca cgggaatatc tgcggcagcg
      121 ggcttgtaat gggttaagtg ataacagatg tctggaaata taggggcaaa tccagctgga
      181 ttggggaatg cagtcacgct tatcgtggat atttaatccg aaaacgggta aaactgtgat
      241 gctggccttt gaccatggtt attttcaggg gccgactacc ggacttgaac gcattgatat
      301 aaatatcgcc ccgctgtttg aacatgccga tgtattaatg tgtacgcgcg gcattttgcg
      361 cagcgtagtt cccccggcga ccaataagcc ggtggtactg cgggcgtcag gtgcgaactc
      421 tattctggcg gaattaagta atgaagccgt ggcgttatcg atggatgatg ccgtgcgcct
      481 gaacagttgc gcggtggcgg cgcaggttta tatcggcagc gaatatgaac atcagtcgat
      541 caaaaatatt attcagctgg tcgatgccgg aatgaaagtg ggaatgccga ccatggccgt
      601 gactggcgtg ggcaaagata tggtgcgcga tcagcgttat ttctcgctcg cgactcgaat
      661 cgccgctgaa atgggggcgc aaattatcaa aacctattat gtcgaaaaag gttttgaacg
      721 gattgttgcc ggatgtccgg tacccattgt tattgctggc ggtaaaaaat taccggagcg
      781 cgagacgctg gaaatgtgct ggcaggctat cgatcagggc gcttctggtg tggatatggg
      841 gcgtaatatt ttccagtctg accatccggt ggcgatgatg aaagccgtac aggcggtggt
      901 tcaccataac gaaacggctg atcgggcata tgaactctat ctgagtgaaa aacagtaact
      961 gcggatttaa ggagaagaat tatgcacgtc acactggttg aaattaacgt tcatgaagac
     1021 aaggttgacg agtttatcga agtttttcgc cagaaccacc tgggctctgt acaggaagaa
     1081 ggtaatttgc gcttcgatgt cttacaggac ccggaagtga attcgcgctt ttatatttac
     1141 gaagcctata aagatgaaga cacagtggca ttccataaaa ccacgcctca ctacaaaacc
     1201 tgtgtcgcga aactggaatc tttaatgacc gggccgcgta aaaaacgtct gttcaatggg
     1261 ttgatgccgt gaggctacaa ttggggtaac gcgctgacgg gagtaaaaaa atgtctgact
     1321 ggaacccctc tttatatctg cacttttccg ctgaacgatc gcgtccggcg gtggagctgc
     1381 ttgccagagt gccgctggag aatgtcgaat atgttgccga tcttggctgt ggcccaggta
     1441 acagcaccgc cctgctgcat caacgttggc ctgcggccag gataacaggc atcgactcgt
     1501 ctccggcgat gattgctgaa gctcgcagtg ctttgccaga ctgccagttc gtggaagcag
     1561 acatccgcaa ctggcaaccg gaacaggcac tcgatctgat ttttgctaat gcctcactgc
     1621 aatggctgcc cgaccactac gaattgtttc ctcacttggt ttctttactt aatccgcagg
     1681 gcgtgctggc agtacagatg ccagataact ggctggagcc gacccatgtg ctcatgcgcg
     1741 aagttgcttg ggaacaaaac tacccggatc gcgggcgtga gccgttggct ggcgttcatg
     1801 cttactacga tattttgagc gaagccggat gtgaggtcga tatctggcga accacttact
     1861 atcaccagat gccgtcgcac caggcgatta tcgattgggt gaccgccact ggattacgtc
     1921 cgtggttaca ggatctgacc gagagcgaac agcagctttt tcttacgcgc taccatcaga
     1981 tgctggaaga gcagtatcca ctgcaagaga acggacagat actgctggca tttccgcgtc
     2041 tgtttattgt tgcccgccgt acggagtaat tatcacgtca gctggtaatg acgatcggga
     2101 agaatttttg ctggaatttc tgcttcatcg ttcatctgta acaggtcaat ttcaatagcg
     2161 ttgcagatgg catccagcgg taaatcattg ttttcagtac cgaacggttc ttccagttct
     2221 tccgccagac aatccagcga aataaaagtg taggaaatca gcacagagat aaaaggcgtc
     2281 atgtaatgca ggtccacgac cagcgcgaac ggcagcatga tacaaaacag ataaacggta
     2341 cgatgcaaaa tcagggtgta ggcaaaggga attggcgtgt aggcaatgcg ctcacagcct
     2401 gccagaaccg ctgaaatatc attaagccga tcgttgaggc taataaacag gatatctgaa
     2461 agctgtccat tgcggcgctg aaccgccaac cattctccca ttattaacaa gatacggtta
     2521 gctggagagt tcgaagccag tacacgctga agatctccag tcttgagata atgagccagc
     2581 acttccgcct gtggctgttt gcgtaatgtc atgcgtaaac agtgggcgaa ggcgatttgc
     2641 agcctggcaa actcccttac acttgccgaa tccggcaatg tcgtttttac ctctcgcagt
     2701 aacgaccgtg aggcaatcat caactgcccc caaagttttc gcgcttcaac gtaacgggcg
     2761 tacccggcat tattacgaaa accaagaaaa atggcgatgg cgacaccgag aatgctgaac
     2821 ggtgcgaggg tgaatttgat gcccagatgc gtgtaccagg gcagcatgaa tataacagca
     2881 atagaaaaga gaaaattgag tagtaagcgc gaggatatct tggataatac tgagccgtgc
     2941 cagacaaaaa tacggcgcag ccagtgttgt tgtggacgaa caatcatggt tatcttcagg
     3001 cgtggaaaag tcgccctatt aaacgtgatt acgatcacat tctcaagacg ctacttacaa
     3061 attacc
//
LOCUS       DAEEGA010000221         2952 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEEGA010000221 DAEEGA010000000
VERSION     DAEEGA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.225"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="KLH46_004214"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="KLH46_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBE0380637.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="KLH46_004215"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="KLH46_004215"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBE0380638.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..>2952
                     /locus_tag="KLH46_004216"
     CDS             2604..>2952
                     /locus_tag="KLH46_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE0380639.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          659 a          702 c          779 g          812 t
ORIGIN      
        1 aaaggggggg ctttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg gtgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgctg tcgattacgg ggggcgaggt gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgttgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccactgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtac tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag cgggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgccagacgc ctcctttctt cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaagct gcggatcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccaaggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg cagcgttaaa tgtctatccg ggccgtgtga
     2701 tggcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aa
//
LOCUS       DAEEGA010000222         2807 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEEGA010000222 DAEEGA010000000
VERSION     DAEEGA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.226"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            6..81
                     /locus_tag="KLH46_004217"
     tRNA            6..81
                     /locus_tag="KLH46_004217"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="KLH46_004218"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="KLH46_004218"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="KLH46_004219"
     CDS             complement(299..1057)
                     /locus_tag="KLH46_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBE0380640.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="KLH46_004220"
     CDS             complement(1065..2168)
                     /locus_tag="KLH46_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBE0380641.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="KLH46_004221"
     CDS             complement(2178..>2807)
                     /locus_tag="KLH46_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBE0380642.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLATAIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          762 a          738 c          676 g          631 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg tggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAEEGA010000223         2745 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEEGA010000223 DAEEGA010000000
VERSION     DAEEGA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.227"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(1399..>2745)
                     /locus_tag="KLH46_004222"
     CDS             complement(1399..>2745)
                     /locus_tag="KLH46_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HBE0380643.1"
                     /translation="IVAAVFLYKLTVASGQFDIIRSLVISITDDQRLQVLLIGFSFGA
                     LLEGAAGFGATVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMM
                     DGWKGVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPK
                     NTETAISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFK
                     ALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAA
                     IISIFILGVGIKKGIGVFAEMLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGT
                     GVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTG
                     KMISPQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
BASE COUNT          858 a          621 c          546 g          720 t
ORIGIN      
        1 tttacgtacc gtttaaacaa agacaatacg tctctttatt tctcataaaa ccccaatccc
       61 ttacagggct tattgaaaaa tttaaaaatc acaaatttct caatactcga aatgagatca
      121 cataagaaat caaaaagcct tctcatctat tttaagtttt cccaacggaa aataagagct
      181 ttcattaata ctgaaccact ttactgttct atttgttcag gtattgcatt acggtgtgct
      241 gagatccatc ggtgcatccc tgtatcccgc gatgagacta aaatagtcgt gatgtaagct
      301 cgtatcagat tcttagtgaa taccacattt gttataatta agtgaaaaga tacattgctg
      361 atttacatta tttggtttac aaaatgtcat cacttgcaga gttatcgctg acagatacct
      421 ttaatctctt actactttcg tgttattgca atacaaccat ccgtaaagca gggctatttt
      481 tagcaggaaa tactcaggtg tcaacattcg aactccaact ctgaaatatt catataagtt
      541 aatgatttta atgattttaa tgattttaat gattttaatg attttaatga ttttaatgat
      601 tttaatgatt ttaatgattt taatgatttt aatgatttta atgatttttt catttattca
      661 cacctaataa aagtattcaa attcagatga tttttctgct caacagacac aaacaggcaa
      721 taatactctc caaagcgaac aatatttggc attacgattt aatgcttcat aatcaccaaa
      781 ttatttaacc ataaattttc ttatttctaa caaaatgatt ggtgtgttgt tggagttaaa
      841 atacatacat aatattaaca tctgccaata tttcactttc cgcaagacgg atgatttcga
      901 atcctcatcc acgttaccct atcccaaata tctttattaa taggcagtaa cgaacactgt
      961 tatagcaaga ttaacttatt aacctaacgg catcttttat gtaacaagtg gagataaatt
     1021 gcttaaaatt gatcctcgac aaatcttaaa agttctcatt attctgcaaa atcagctttg
     1081 cgaataatct tatgtttttg ggagttttgg gaattttgat ctactcattg ttctagtgaa
     1141 aaatataaat aagagaaaaa acgccattcg tcatgaaagc aacagatgaa gcaatgtaat
     1201 aacatactaa aattgatctc atgaaagtgg gtactttata accagcacag aagccaagtt
     1261 gtataaatat taaacaattc gcatcgtaaa acggagacta cgtataaaga agccaatcgt
     1321 aaaaacggtt ccctggcgag ataaacgaca gacaaatgga aagccgggtg atatttacct
     1381 tcatccggct ttattgattt acgagactaa catcccggta aacacatacg cctgcagcag
     1441 ggtgataacg ccgataacgc tggcaaaaat cagactgtgc ttcacggtgt agcggaacag
     1501 ttcagattct cggcccacca tgcccgtcgc ggcgcaggcc acggcgatag attgcgggga
     1561 gatcatcttg ccagttacgc cgccgctggt atttgctgcc accagcagag tgtcagagac
     1621 gttgatttgc tgcgccgtgg tcgattgcag tgaaccaaac agggcgtttg aagaagtgtc
     1681 cgacccggta aggaatacgc ccagccagcc gagaaacggt gagaaaaacg ggaacatcac
     1741 gcctgtacct gccagtacca gcgccagcgt ggtggacatg ccggaatagt tggtgacgaa
     1801 ggcgaatgcc agcaccatgc caatcgacaa tatcggccac ttcagactaa ttagcatttc
     1861 ggcaaagacg ccaatccctt tcttgatgcc cacgccgagg atgaagatag agataatcgc
     1921 cgcaataaaa atagcggtgc cgccagccga gagagggtcg aatttaaaca ccgcatccat
     1981 tggcgttggt tgggcgacaa tgggtgccgc tttcaacact tgttgatgca aatgagggat
     2041 ctggaaatta atcaccagtg aataaaacgc gccgcccgga gcaaataacg ctttaaacgg
     2101 cttcatggtc cagatggtga ccagcaccgt taagattaaa aacggtgacc acgcacgaat
     2161 gatttgcccc agactatatt ctgaaggcac gggaccgcca gaagatggct tatttaccac
     2221 catcgcacct gcggattgcc ccatgctgat tgccgtttcg gtatttttcg gccgccagac
     2281 tttaagaaat aaagcgagtg agacgatact caccagcgcc gaagtaatat ccggcagttc
     2341 cggaccaata tagttagagg taaagaactg agtgacagcg aagcttcccc cagcaaccag
     2401 cgccgctggc cacgtctctt tcaccccttt ccagccgtcc atcattgcta ccagccagaa
     2461 cggcacaaga accgacagga acggtaactg acgtcccgcc attgcgccaa tgtggaacgg
     2521 atcgattccc gttacctgac cggcgaccag aatcggcacg cccaacgcac caaacgccac
     2581 cgtcgcacca aagccagcag ctccttccag caacgcacca aaggagaaac caatcagtaa
     2641 cacctgcaaa cgctgatcgt cggtgatgga gataaccaag ctgcggataa tatcgaactg
     2701 cccgctggca acggttaatt tatacaggaa caccgccgcg acaat
//
LOCUS       DAEEGA010000224         2730 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEEGA010000224 DAEEGA010000000
VERSION     DAEEGA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.228"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(220..384)
                     /locus_tag="KLH46_004223"
     CDS             complement(220..384)
                     /locus_tag="KLH46_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380644.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="KLH46_004224"
     CDS             1286..1570
                     /locus_tag="KLH46_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBE0380645.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="KLH46_004225"
     CDS             1558..2043
                     /locus_tag="KLH46_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0380646.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
     gene            complement(2216..2497)
                     /locus_tag="KLH46_004226"
                     /pseudo
     CDS             complement(2216..2497)
                     /locus_tag="KLH46_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
     gene            2564..>2730
                     /locus_tag="KLH46_004227"
     CDS             2564..>2730
                     /locus_tag="KLH46_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0380647.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPG"
BASE COUNT          721 a          585 c          745 g          679 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataaggg ctgagggatc cccacaaaat tactgctaat
     2221 aaaccagcac catatttcgg taggttctgg acagatggtt tagaactgtg ctcagtactg
     2281 ttcgtcttaa aataagtggc gagtgtcgaa gaacattctt cgctaacgtc agatatgaga
     2341 taacccgccg ggatttaatg ctgttagcct gatatttcag gtgtaaccct ttattttcag
     2401 cgtgataacc gagtaaccac attacgatag tgctcaacgt tgccagcaga ctcagaacca
     2461 gaattcttcc tgttgaacga cttttgctgg cccgaagact gaggtagcct gagtttaacg
     2521 gacactcctt cctgaaatag aatggcatca gaaggagcta ataatgagca gaaaaaccca
     2581 acgttactct aaagagttca aagccgaagc tgtcagaacg gttcttgaaa atcaactttc
     2641 gatcagtgaa ggcgcttccc gattatctct tcctgaaggc actttaggac aatgggttac
     2701 cgccgccaga aaagggctcg gtactcctgg
//
LOCUS       DAEEGA010000225         2718 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEEGA010000225 DAEEGA010000000
VERSION     DAEEGA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.229"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..437
                     /locus_tag="KLH46_004228"
     CDS             <1..437
                     /locus_tag="KLH46_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0380648.1"
                     /translation="NRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPT
                     VRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEM
                     VRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
     gene            786..1958
                     /locus_tag="KLH46_004229"
     CDS             786..1958
                     /locus_tag="KLH46_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBE0380649.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2012..2635)
                     /locus_tag="KLH46_004230"
     CDS             complement(2012..2635)
                     /locus_tag="KLH46_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0380650.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT          837 a          564 c          521 g          796 t
ORIGIN      
        1 caaaccgcgc aattggcgtt gcgctgaatg atactgcatg tcgcgtattg aaaaaacaaa
       61 tcggaaatca tcaccgttgg gtatttgtgt acaaggaaag ctgtaccaaa ccagacggaa
      121 cgaaagcgcc aacagtaagg aagatgcggt atgacgcaaa cacagcctgg aaagcggcgc
      181 tgagacgggc tggtattgat gatttcagat ttcacgactt gagacacacc tgggcaagtt
      241 ggctggttca agccggagtc ccgttatcag tgttacagga aatgggaggc tgggagtcta
      301 tcgaaatggt tcgtcgatat gctcacctcg cacctaatca ccttaccgaa cacgcacggc
      361 aaatagactt gattctgaac ccatcggtcc caaatttgtc ccagtcaaga aataaggaag
      421 gtactaatga tgtgtaactt attgatttaa atggtgccga taatagcagt cgaacctacg
      481 accttcgcat tacgaatctg tagcaccaat cataactatc tgttttagca agcattaacc
      541 gcattcacta agctatagtt gatgacacaa acagaaagtt gatgcatgat gttgccatat
      601 atatgtcaca aatacggcac aacgatcttc aaacatatag ccacacattc acagaagagc
      661 acaaagcctt gcaatccagt gcaaagcttg gtgtgtctcc gttttgtttc atcagttaca
      721 cataactata tgatcattaa ctaaaagtca gtatacttgg agaaatttta attgcgtaat
      781 agagcatgca tatgattgaa attaacagtt tattattaat aacatccgtg atattgatgt
      841 cgttattagc tgtggggtta tttgataaaa tttccccaat aaatcttgtt gagcacggca
      901 ggaataatca aatagatgga atgcgggggt tcttagcaat tttcgtgctt attcatcacg
      961 cagcaatttg gaatggatac ttgtcatctg gagtatggga agcaccttca tcaaatctgt
     1021 tagcaaattt aggccaagtt ggtgtgtcat tcttttttat gattactggt tatctgttct
     1081 tttcaaagat tatctctgga gatcaggact ggacaaggct ttatgtatca agattactac
     1141 gattaacccc aatgttcata gttagcctat gtctcatttt tatcattgta ggttttaagt
     1201 ctggatggag aatgcaggta tccacagaag agctttttgt gtcaataatg aagtggttgc
     1261 cattcactgc gctaggtatg ccgaacatta atgacgtaaa agattcattt actatcaatg
     1321 ccgctgtaac atggacactt gtatatgaat ggttctttta tttttctctt ccggtaattt
     1381 ccgcgctcat aaaaagaaaa gtcagtattt acatggttat gattagcgca atatcgctat
     1441 ttgttttcat tttatttttc agtaaaatac acatagcatc atttcttttt gggctcctgg
     1501 catttttgct aaataaatca aagatagtca atggaattgc caaagcaaaa gtaacaccaa
     1561 ttataataac tgcgataatg gtttttgaaa tgacgtattt caaaacaact tacgcaccgc
     1621 taccactgat tctttgtgga ataacattta tcattatcgc atcaggttgt gatttgtatg
     1681 gaatattaag attaaatata accagaaagc taggagaaac tacctacagc gtttaccttc
     1741 tgcatgggat attcctttat tgcctaatga cgtggattat tcctaataat tacactgaaa
     1801 atacatttat catactggta agcacaactg catttcttat tacgttcaca tcatgcctaa
     1861 cttttaaatt aatagagacg ccattcatca aattaacaaa acaaaccaca actttagtaa
     1921 aagaattaat acccacatta acaaacaaca atcaataaac aataaacata agccgcccta
     1981 catatatgtc caaatggggc ggcgaatgta tttatattag tctggtaatg ctaaatgcaa
     2041 acgaactgga gattcctgcc ccgctaagcg tattgcgcag ggtcacccta aatgtatcta
     2101 cgccaatata agtaacatca caactaactg aatcaggcgc agatataggt gttatctgca
     2161 caatataacc gctagcaact gctgcgagcg gatgagaaaa gctaacatca tacacacctg
     2221 tagaaaccac gttaaccgat gaaacaccag tagcaacgtc gatactacca ttggcgttaa
     2281 tttttcccga gcgtgtgact gacaatgcag aagccgatgt taccttactg gttgtttctg
     2341 taaaaacaca cccaatgtca actacctcgc aattagctcc tgttatccag tatggtcgtt
     2401 ctgcacttgg ctgataatcc cctgcgctct ggaacgttgt gccgttgagt atgactgtaa
     2461 cttttccccc acctgagctt gttacttgaa tattcgtgtg cgtgtatctt gcactgctca
     2521 cgcgatgaaa atttccaccg tttataacca cagttacagg gcgcgtccca gtattatcta
     2581 tagcaaggtc tgcccccccg gcattagcct caaagtatgg gttgtttaat accaaagcgc
     2641 acgaaccgtt aaggccattt atagctaatt gcgcaccacc tgtactggaa tccccctgag
     2701 taccatttcc ttcacaag
//
LOCUS       DAEEGA010000226         2690 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEEGA010000226 DAEEGA010000000
VERSION     DAEEGA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.230"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            230..769
                     /locus_tag="KLH46_004231"
     CDS             230..769
                     /locus_tag="KLH46_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBE0380651.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="KLH46_004232"
     CDS             complement(995..1318)
                     /locus_tag="KLH46_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HBE0380652.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="KLH46_004233"
     CDS             1604..1798
                     /locus_tag="KLH46_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBE0380653.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcagggg caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttgatagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAEEGA010000227         2644 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEEGA010000227 DAEEGA010000000
VERSION     DAEEGA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.231"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..570
                     /locus_tag="KLH46_004234"
     CDS             <1..570
                     /locus_tag="KLH46_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBE0380654.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMP
                     VYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKD
                     FEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            571..936
                     /locus_tag="KLH46_004235"
     CDS             571..936
                     /locus_tag="KLH46_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBE0380655.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVC
                     GQLVPGGRLGIKITELGCA"
     gene            933..1616
                     /locus_tag="KLH46_004236"
     CDS             933..1616
                     /locus_tag="KLH46_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBE0380656.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1815..1890
                     /locus_tag="KLH46_004237"
     tRNA            1815..1890
                     /locus_tag="KLH46_004237"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1848..1850,aa:Ile,seq:cat)
     gene            1892..1968
                     /locus_tag="KLH46_004238"
     tRNA            1892..1968
                     /locus_tag="KLH46_004238"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1926..1928,aa:Lys,seq:ttt)
     gene            1971..2046
                     /locus_tag="KLH46_004239"
     tRNA            1971..2046
                     /locus_tag="KLH46_004239"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2004..2006,aa:Thr,seq:tgt)
     gene            2050..2124
                     /locus_tag="KLH46_004240"
     tRNA            2050..2124
                     /locus_tag="KLH46_004240"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2083..2085,aa:Gly,seq:tcc)
     gene            2417..2632
                     /locus_tag="KLH46_004241"
     CDS             2417..2632
                     /locus_tag="KLH46_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBE0380657.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          691 a          581 c          715 g          657 t
ORIGIN      
        1 gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta
       61 ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac
      121 atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa aaatcttgcg
      181 ccgctgcctc acgcccacca gcaacagaat ccaccacagg aagagacggt ggtcagcatt
      241 gccgttgatc cggaatctcc ggctcagtat ctccagcgcc agaaaccaca acgggaagag
      301 atgcctgtat acacgcgctg ggtaaaaacg cagaaatgca tgacgtgtgg caatcaggca
      361 gatgatccgc accacatcat tggtcatgga ctgggaggga tgggaacaaa ggctgacgat
      421 ttgtttgtta ttccgctgtg tcgtaaatgc cataacgaac tacacgccgg ggtaaaagat
      481 tttgaagaaa aacacggtag tcagttgttg ttgctgattc gttttttaat gcacgcgaga
      541 aattcgggtg ttctgaagtg gaaagcataa atgaccgaac gcatagaatt tgttttgcct
      601 tacccgccaa cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      661 gccggtgagc gttatcgccg tgatgtggcg ctaattgttc gccagcagcg actgaaatta
      721 aacctgtccg gaaggctggc gataaagatt attgcagagc caccggataa gcgccgtcgt
      781 gacctggaca atatcctgaa ggcaccgctg gatgcgctga cgcatgctgg actacttata
      841 gacgacgagc agtttgatga aatcaatatt gtgtgcggtc agctcgttcc tggtgggcgg
      901 ctggggataa aaatcacaga actggggtgc gcatgaataa ccagtattta cagtttgtgc
      961 gtgagcagct cattatcgcc accgctgatt tgagtggggc aacaaaaggt cagcttgaag
     1021 cctggcaaga gaatgccatg ttcgatacag ggcgttacag gcgtaaaaaa atccggtacc
     1081 gcgatgaagt gactggaaaa atgataacgc gggataatcc accaatcccg ggaaagcaat
     1141 cgctggcgaa ggggacgtca attcctctgg tcagtccggt tgagttttcg acatcatcgt
     1201 ggcggcgggc tgttctgtct cttgaagagc actataaagc ctggttgttg tggtgttaca
     1261 gcgggagtat ttgttgggaa tatcagatcg cgataacaca gtgggcgtgg aatgaattta
     1321 atgctcaatc cggtaccaga aaaattgcag ggaaaacgca ggaacgcctg aaaaaattaa
     1381 tctggctggc ggcgcaggca gtaaaagcag aactttttgg tggggaaggt tatgaatacc
     1441 aggagctggc attactggcg ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc
     1501 actgggttgc aatgaaacac atttttcacc gactggatag tgaggcttta ttgtttgtaa
     1561 tgagaacacg ttcaaaaaaa agacggcatt ttcaaagcaa agtgttgcaa aagtagattg
     1621 aaaggcatat attcatgcaa atctgatact ttgccgattt tgtacgtgat ggcaaaagca
     1681 aacaaaaccc gcccacaagc gggttttttg tgccacttat ctcggataga catggtgaat
     1741 gcgctggtgg aggagataag ggtgattttt gaatgcttgc aacattgatt tcgtaacgtt
     1801 attatcctgc gcccggccct ttagctcagt ggtgagagcg agcgactcat aatcgccagg
     1861 tcgctggttc aaatccagca agggccacca accgccacta gctcatcagg aaagagcgtc
     1921 aaccctttaa gttgagtgtg cgaggttcga gtccccggtg gcggtccagt gccgacttgg
     1981 ctcagtaggt agagcaactg acttgtaatc agtaggtcac cagttcgatt ccggtagtcg
     2041 gcaccatatg cgggcatcgt ataatggcta ttacctcagc cttccaagct gatgatgcgg
     2101 gttcgattcc cgctgcccgc tccagtcaga gtctttcagt ctgcgatgat gggaaatccc
     2161 ggagtgactg aaagacgttt aagttatgaa tgatcgcttt tttttgcaaa attgctgtgc
     2221 agaaatacta accttcgggc aggcgatcat tcataagcac tctgctttta ttccgattaa
     2281 ctgtgagtgg tttgttggat agagtgcttt ccttactgta tatatcgttt cgcccgcttt
     2341 tgcggttttt tcttttcaaa tccctttcat ttctcagtgt aaaactacgc catccgttat
     2401 ttgcggaggt gaggctatga aatccatgga caaaatttca acgggcattg cctacggcac
     2461 ctccgcaggc agtgctggct actggttttt acagtggctt gatcaggtca gtccgtcaca
     2521 gtgggctgcg attggtgtac tggggagtct ggttctgggc ttcctgactt atctgacaaa
     2581 tctgtacttc aaaatcagag aagacaagcg taaggctgcg agaggtgaat aatgtcgcca
     2641 tcat
//
LOCUS       DAEEGA010000228         2567 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEEGA010000228 DAEEGA010000000
VERSION     DAEEGA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.233"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..489
                     /gene="capU"
                     /locus_tag="KLH46_004242"
     CDS             <1..489
                     /gene="capU"
                     /locus_tag="KLH46_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBE0380658.1"
                     /translation="EFMLNLLFHLKMNGRQFCWLIVGSGSPELREHLQYQIDSMGMHD
                     DVFIADNVFPAAPVYRVASLVVLPSENESFGMVLAEASAFSVPVVATQIGGIPEVIQN
                     NQTGTLLPASNKHAWMCALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAK
                     QK"
     gene            494..1444
                     /gene="virK"
                     /locus_tag="KLH46_004243"
     CDS             494..1444
                     /gene="virK"
                     /locus_tag="KLH46_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HBE0380659.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1509..2453
                     /gene="lpxM"
                     /locus_tag="KLH46_004244"
                     /gene_synonym="msbB"
     CDS             1509..2453
                     /gene="lpxM"
                     /locus_tag="KLH46_004244"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBE0380660.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          741 a          489 c          582 g          755 t
ORIGIN      
        1 gaatttatgt tgaatttact gttccattta aaaatgaatg gacgacagtt ctgttggttg
       61 attgtggggt ctggttcacc tgaactgcgg gaacatttac agtatcagat tgacagtatg
      121 ggcatgcatg atgatgtttt tattgctgac aatgtttttc ctgccgcccc cgtatatcgg
      181 gttgccagtc tggtggttct gccttcagaa aacgaatctt ttggtatggt gctggcagaa
      241 gcatcggcat tttctgtgcc tgtagtggcc actcagattg gtggaatccc tgaggttatt
      301 cagaacaacc agaccgggac attgttacca gcaagtaata agcacgcatg gatgtgcgcc
      361 ctgaatgatt tttttaatga ccctgggcgt ttttatcaga tggctcgcct ggcaaaacag
      421 gatatagaag agcggtttga tattaataaa actgcgttaa aaatactcac attagcgaag
      481 caaaagtaac aatatgtttt ctataagtaa cttatcattt atcggtttcc ttaaaaggat
      541 tattttttcc tcagattcac taccggggaa gtgggaacac agaaaatttc ggttcatgta
      601 cattttgcga tgttctataa atccggttgt cagtattcga tattattatg aactgcgttc
      661 cttgccgtgc attgaggata ttctggcaat acaccccacg ttgccagcaa gaattcatcg
      721 cccttatttg cataaagggg ggcgcgcctg gacccgtgga caatatattc ttgagcatta
      781 tcgctttgtg caaaatttgc cagaaaaata ttctaaattt ttgtttccac aaaaatcggt
      841 atcattagtc caattcatcg ggaaagatgg agagaatttt gatatccagt gttctcccag
      901 tggttttgac cgggaaggag agttgatgtt gtcattgttt tacaataaaa cagttattgc
      961 ccggttaaca ttttcagtca ttctgactca aaatggacac attgctttta ttggcggatt
     1021 acagggagcc cctaaaaata ccggacctga tgttattcgg tgcgcaaccc gggcctgtta
     1081 tgggattttc ccaaaacgga ttatttttga agcattttgt gcgttgatga aagcttgcaa
     1141 tatctctgag tgtctggcag tcagtgagca cagtcatgtt ttccggcaat tgagatactg
     1201 gtatcagaaa cgcaaaacct ttgttgcggt ctatagtgat ttctgggagt ctgtagcagg
     1261 aaaaacctgt ggtgactggt acagattacc aacccaggtg attcgaaagc cgctaagcga
     1321 tatagccagt aaaaaacgct ctgagtaccg aaaacgatac gctttgctgg attatatcca
     1381 tgaaactact atccgctctt tggatgcata tcctgtacac tcagaacatc agaatttaaa
     1441 ttaaggtttg atgtattctc tgaataaaac agtaatgata attttggtag aggcattcgc
     1501 actaaataat gaaaaaatat aaatctgagt ttattcctga atttaagaaa aattaccttt
     1561 cccctgttta ctggtctaca tggttccttt tgggaatgat tgcaggtatt tcaatgtttc
     1621 ccccttcatt cagagatcct gtcttggcaa aaatagggcg ttgggcgggc agattgagca
     1681 aaaaagctcg tcgcagggcg acgattaatt tatcgctttg tttcccagaa aagagtgata
     1741 cagaacggga aataattgtc gacaaaatgt ttgtcacagc attgcaatcc atagtgatga
     1801 tggcagaact ggcaattcgt ggtccggaaa agttccagaa acgagtgttc tggaaaggcc
     1861 tcgaaattct tgaggaaatt cgacacaata atagaaatgt gatttttctg gttccccatg
     1921 gctggagcgt ggatattcct gcaatgttgc tggcagctca gggaaaaaaa atggccgcca
     1981 tgtttcatca gcaacgaaat ccagtgattg attatgtctg gaattcagta cggcgtaaat
     2041 tcggggggcg cttacatgcc cgggaggatg ggataaaacc atttattcag tcagtacgcc
     2101 agggatactg gggatattac ctcccagatc aggatcatgg tcctgaatac agtgaatttg
     2161 ctgatttttt tgcgacctat aaagcgacat taccaatcat tggacgtctg atgaacatca
     2221 gtcaggctat gattatacca cttttcccgg tttatgatga aaaaaaacat ctactgacta
     2281 ttgaaattcg gccaccaatg gatgcttgca ttgccagcgc ggacaataaa acgattgccc
     2341 gacaaatgaa caaaacagtg gaaattttgg tggggccaca tccggaacag tatgtctggg
     2401 ttttaaaatt gttaaaaaca cgcaaatcaa acgaagcgga cccgtaccct tgaaattata
     2461 aagtacaagt atttccacta gttgtttgta agcgtacagc ctgaaccgtc tggtcagaat
     2521 ctgacgaatt agacaaagtg gtgtccacca aataagtagt gggaacc
//
LOCUS       DAEEGA010000229         2540 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEEGA010000229 DAEEGA010000000
VERSION     DAEEGA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.234"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..87
                     /locus_tag="KLH46_004245"
     CDS             <1..87
                     /locus_tag="KLH46_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0380661.1"
                     /translation="GRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            171..1649
                     /locus_tag="KLH46_004246"
                     /pseudo
     CDS             171..1649
                     /locus_tag="KLH46_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            1646..>2540
                     /locus_tag="KLH46_004247"
     CDS             1646..>2540
                     /locus_tag="KLH46_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBE0380662.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQ"
BASE COUNT          661 a          602 c          629 g          648 t
ORIGIN      
        1 ggacggaagt cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt catcgggcat
       61 tatctgaaca taaaacacta tcagtaagtt ggagtcatta cccgcttact cgtgaggatt
      121 tatgttctgc tggcagtttc gtgatcaaca cctcgcgcca ggatggcttt atgcactatc
      181 gcaatgtggt ttgtttgttg tcttgttcat tgtttttatc gtcggcatgg ggatgtcggc
      241 tttccgcacc tgaacataat atttaccaga aacagggaaa aggcgtggtg tacctgcgcc
      301 cttataaaga aacaaatctt tctctacctg acgtgaatta caagagtttg cgtcggttac
      361 ccaatttatt aatagacccg acaacactag acgaatggga taaagaaccg cctctgactg
      421 atttgacaac cgactacctc tatgagggcg cacaggcctg gtatcctcat tatccatggc
      481 atagcgatgg tcgcaatatc ctctacgcgg gtgaagttgt gcaaaaccct cccggcacac
      541 ctcccgtcga tgtcgcctcg ttcaaggcat ggggtgattt tgccgcagat aaacacagtc
      601 tctattttga aggcaagcgc accgatgaca atggtggtgg aaatagcttg gatatcaaaa
      661 cgctgcatca ggtggaattc cggcctccct gggatcctga cttactgggg ctgatattac
      721 gtgacgctaa tttcttgtat atcaatgggc atcgcctcgc tgaccccgag agtttccgtg
      781 tgctggcgca gaagtcctgg gatcagcgag gaaagttttc caccacgttt aacccctgca
      841 ttgccgtacc atttggcccc tgggataccc tggctcgtac acggaccaaa atcctgctca
      901 acggcgaaca gcttgatgcc gacccggaca ccttttccgt cgtgcgctgg atgcccggct
      961 cactcttgac ctggcgtgat aaaaacgggc tgcagcgtaa agtcctcgac aaggaaaatc
     1021 tggcgtggga tgaagattta acaaagcact gtctggattt ttctctgctg gaaaagaaag
     1081 tgttctggcg taaaggacct gcttgtaaac aggaagaatt acccggactc gatccggagc
     1141 agtttcaacc catcagtgat gctgtcgccc agcatcagga ctcgctttat accatcatcg
     1201 aaacagagtc tggtgaccgt aagctggaga tcgtgaaact tgatgatccc aatcttatta
     1261 tcaacaaacg tttcaacgcc gggaaacgcc acggctattt acttacgcgt gccgaagggt
     1321 agccatacca ttccggttta cacgtgtttg aatctgacgg accgctgatc ttactggata
     1381 accgttctcc ggatgaacgc gaagcccatc ttaatgacca tccctttttg cgcagatggt
     1441 atgcccgcga taaccgctac atttacagct ttgatggcgc gcagctctgg cgataccgca
     1501 ccgctgatcc gaaacaagtt cgcttaatct ggaaggaaca acattcggga tatgtctatg
     1561 gcgtaaatta caaaacggga tatctggacg gaaaaattac tgatgacggc gaatttattc
     1621 ctgccccgcg caatgaggcg acaaaatgaa gggtcaaata atcacagttg cactcatcct
     1681 gttgggacac acatttctga cgcctgctgt acaggccata gggttcgact attacaatga
     1741 tcacggtgtt atgtcttacg gtaaaggtta ttggggggat aagaaaatca ttgcccagtt
     1801 tccggagatg agcagggccg gtttgcgcct tgtgaaaaat atctctggga aaacgggtta
     1861 tataccttcc gatgaaataa gcatagaaaa tgaggaatac cactgggtga cagacggccg
     1921 cgttattctc tggcgtggca aaatcgttag caatccaccg ggaacactca ctgtcgatat
     1981 tgccagcttt caggctatgg gccgcttcgc ggtcgataaa tatagcctct attttgacgg
     2041 acagcgcacc gaaagtaata gcggcgcgtc acgtgttgag ctggcgacac taaaagctat
     2101 cgaaggtaac tctaccacgc tggtggatag taaaaatctc tacttgtccg gtcgacgtca
     2161 ggggagcagc agtgatgtta ctgtattaga aaaaagatgg tggggtatta atccacgtct
     2221 tatgagtgta aacagaaatt catattccaa tgatctgctt atccgcagtg ggcagaatat
     2281 ttatttaaat ggcgttcacc ttacggcgaa tgcagactca tttgagataa ttcgctggat
     2341 acctcattca ctgctggttt ttcgcgacaa taagggtctg catcgttatc cctttggtca
     2401 attatcaggc aaagcgatac ccgtagatga tgacgtctct tttgaagtag gggaaagtcg
     2461 cgttcgctgg cgtaaacagc tcacgcccga ccgtcagtgg agcaagtgga tagacctacc
     2521 aggtattgaa cctgaacaat
//
LOCUS       DAEEGA010000230         2490 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEEGA010000230 DAEEGA010000000
VERSION     DAEEGA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.235"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            63..284
                     /gene="kilR"
                     /locus_tag="KLH46_004248"
     CDS             63..284
                     /gene="kilR"
                     /locus_tag="KLH46_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HBE0380663.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            278..454
                     /locus_tag="KLH46_004249"
     CDS             278..454
                     /locus_tag="KLH46_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380664.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            529..804
                     /locus_tag="KLH46_004250"
     CDS             529..804
                     /locus_tag="KLH46_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380665.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            904..2319
                     /locus_tag="KLH46_004251"
                     /pseudo
     CDS             904..2319
                     /locus_tag="KLH46_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
     gene            complement(2376..>2490)
                     /locus_tag="KLH46_004252"
     CDS             complement(2376..>2490)
                     /locus_tag="KLH46_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0380666.1"
                     /translation="SVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          759 a          567 c          570 g          594 t
ORIGIN      
        1 gctaagtatt aaataacttt cctgagattt cctttgtaag actgtttatt aaggaggctt
       61 ccatgattgc tcatcacttc ggaactgatg aaataccacg tcagtgtgtg actcctggcg
      121 attatgttct tcatgaaggt cggacatata tcgcctcggc aaacaatatt aaaaagcgaa
      181 aactttatat tcgtagcctg actacaaaaa catgcatttc tgactgcatg attaaagtct
      241 tcctcggtcg tgatggttta cctgtaaagg cggagtcatg gtaatgacta agaaaataaa
      301 atgtgcttat cacctttgca ataaagaaat tgaagaaagc aaaatcatta caagaccact
      361 tcatttcatg cgtggagtta taccaacgac ggaaatgaaa aaatattgta gtgaaatctg
      421 tgccgaaaaa gaccagatgg cacacgaact ttaattaact gactatccga aactgaattt
      481 atgccagcaa tggcagggat tcgctcaacc ttaattaagg agaaaaacat gattaccagt
      541 tatgaagcca ctgttgtaac tactgatgac attgttcacg aggttaatct ggaaggaaag
      601 cgtattggct acgtgattaa gacagaaaat aaagaaaccc cattcactgt ggttgatatc
      661 gacggtccat caggcaacgt taaaacactt aacgatggtg taaaaaaaat gtgtctggtg
      721 cacataggaa agaatctgcc cgcagaaaaa aaagccgaat ttctggcaac tctgattgca
      781 atgaaattaa aaggtgaaat ctgaaaaaaa gaaagcctgc acactgtgca ggcctgaatg
      841 aagaacctgg gacatttatt catcactcgc agtaatttta atctgagttg aggttaaaaa
      901 acaatgaaca ccgataaaca agtttaccca ctgtattacg aagcaaaaaa tgacaaagta
      961 agaaaacgtc tcggtattaa aggcggtttt tactgggctg aagcgaaaaa attatccatt
     1021 gccatctccc gtggtgctgt tgcgattgac gatgcaggct acgatgaaga tgactttaaa
     1081 aaacctgttc gcgtcaattt gcccgttgtt gatgacctcc cgccagaagg cgtatttgat
     1141 acggaattct gcaaccgtta cgaaaaaggc ggggaagatg gcatcacaat ggtatttatc
     1201 gcatcttctc cctctgttca ggacaaaccg gccagcactg acaataccaa cgtcaacggc
     1261 gaagacatga cagaaattga ggagaacatg ctcctgccag tttccggtca ggaactgccc
     1321 attcgctggc ttgcgcaaca cggcagcgaa aaaccagtaa cgcacgtttc acgcgacgaa
     1381 ctccaggcat tacacattgc tcgggatgaa gaactaccag ctgttactgc cctggctgtt
     1441 tcccacaaaa ccagcctgct cgatccgctg gaaattcgcg atctccacaa actggtgcgt
     1501 gatactgaca gagttttccc taatccaggc aattcaagcc tggggctgat gactgctttt
     1561 ttcgaagcat acatggacgc agactacacc gatcgcggtc tgctgacaaa agagtggatg
     1621 aaaggaaatc gtgtttcacg catcacgcgc acggcttccg gcgctaatgc tggcggcggg
     1681 aacctcaccg atcgcggcga aggtttcgtt cacgatctga cgtcactggc gcgcgacgta
     1741 gccactggcg tactggctcg ttcaatggac gtggacattt ataaccttca tccggcacac
     1801 gctaaacgtg tcgaggaaat tatcgctgaa aataaaccgc ccttttctgt tttccgcgac
     1861 aaattcatca ccatgcctgg cgggatggat tattcccgcg ccatcgtggt tgcgtcagtg
     1921 aaagaagcac cgattggtat cgaggtcatc cccgctcacg tcactgaata tctgaacaag
     1981 gtactgactg aaaccgatca tgccaaccct gatccggaaa tcgtggatat tgcctgcggt
     2041 cgctcctctg ccccgatgcc gcaacgtgta acagaagaag gaaaacagga tgatgaagaa
     2101 aaaacgcagc catctggcgc aatggcagat gaacaggcaa cggctgaaac aatggaaccg
     2161 gatgcaactg aacatcatca gaacacgcag ccgctggatg ctcagtcaca ggtaaattct
     2221 gttgatgaga aatatcagga actacgggca gaactccatg aagcccggaa aaacattccg
     2281 cccaaaaatc ctgtcgatgc agacaaatta ctggctgcct tgaggtagcc tgtgaggtgt
     2341 actggcaata gcggacacta ccatttgttc tttttttaag cagccatctg atgatatttt
     2401 tccctgaagg ctgccgggga gatattcccc agacgagagt gacgacgctg acgattgtag
     2461 aaaatctcaa tgtattcccg tattactgag
//
LOCUS       DAEEGA010000231         2458 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEEGA010000231 DAEEGA010000000
VERSION     DAEEGA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.236"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..797)
                     /locus_tag="KLH46_004253"
     CDS             complement(<1..797)
                     /locus_tag="KLH46_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBE0380667.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLT"
     gene            complement(1397..1645)
                     /locus_tag="KLH46_004254"
     CDS             complement(1397..1645)
                     /locus_tag="KLH46_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBE0380668.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2000..2347)
                     /locus_tag="KLH46_004255"
                     /pseudo
     CDS             complement(2000..2347)
                     /locus_tag="KLH46_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
BASE COUNT          632 a          463 c          595 g          768 t
ORIGIN      
        1 gttaatcttt gcagggattg cagagcatgg gatgacaatg ggttatcact aatatctatt
       61 gaacattcat tcctcagatt aagaatgctt tccgggatat gacttatctg atttatgtca
      121 aaataaccgt tccttaaatt ttccggcagg tatggaatat cggttaacat attgtcgatt
      181 gcccatatgg agtgaatgtt gtgaggtaga ggggggagtt cactaagaag attccctatt
      241 gcgctaatct cttgtaaaga aaatggtaaa ggtggtaaaa ctgccagccc attacatgat
      301 acatctaatg tttccagtag ttctggtaaa acaggaagag aacataattg attatttgag
      361 acatgaagct ccttcaaaga tatggggagt gtgggtagtg tgattagttg attgtgggac
      421 gcattcaata atttaagtcc ttgaggcaaa gcaggcagtt caataagtct gttatagctg
      481 acattaagtt gtgtaaggga cgcaggtaat ggggagatta agcttaaatt atttttactt
      541 atattaattg atttaattcc cggggggatt tcaggtaatg ttgtcaggcc taattcagac
      601 aagtccaggt tcgtctcttg gttttgtaga catgatacta gtcgctgaaa agcgatgtct
      661 cgttgttctt cttgtatgcg gttatttttc cattcagtcc aatgggttag gtaattttca
      721 tatgcgcggc tagtgtcgat tgaataagtg gagaacgaat ttgaaattaa tctgtgattg
      781 ttatttgtcg ggagcataaa tatcagggcg tattctttat gttgagaggt gttggattct
      841 tttttatctg ctttgcaatg ttattggttc ccttcagcag tagacaggaa cttctggagg
      901 aggcggatat atagtttgtg taagcatatt aactccatat gttatatatt gaagaacttc
      961 tgctttacac tatcctaaac tggatcggtt taatgtaaaa aaacgatact ttttgggggg
     1021 ggggcaattt ttccttaagg tatgaaaaaa ggagctgaag ctatataata gctattaatg
     1081 ccactggtat cagtgaatca tgtatataaa aaactgtgtt aaggaggctg tccattagcg
     1141 gttttttatt cttctggaac cgcggcatct ttgcgtatct ttttgtcttc tcaccgtctg
     1201 gtcggtgttt tgctgagact gctaatttcc tgtctttgct agtgctgtac tggtactgcc
     1261 caatcatgat tggcgggggc gggcggagtt gagaccgcag acacgtcgta tgcaagaacg
     1321 tgctgcggct ggctggtgaa cttccgatag tgcgagtatt gaatgatttc cagccgttac
     1381 tgattttacg tgctaattag tgaacaaacc actcgtcagc agactcccag gtatctttca
     1441 gagtttcctg aacaaaagtt ttagctgaat ctttatcggc ggtgcgcgta acagaaaggc
     1501 catcgttgct ggtggctttt acgatcacct ctacatcgtc ataacgttta ctgatgcgtc
     1561 gggttaattc ttcctttaac gcatccacag caccggttgg cattttagtc attttctctt
     1621 tggctatgca gatttcaaaa cgcataaaaa tccctctgca ctgtgttttt atacagtatt
     1681 atttttaact gtatggataa acagtgtcaa gagtgaggga aagtgatttt ttggggcatg
     1741 catggggcaa aaatatttgt tgtggggcat tttggggcat gattgggaca ctttgttaca
     1801 tatgaacttt gccgattttc atatgacatg gcaatccaac gctttgatta ttctaaagaa
     1861 aacacatgct cttgggcgtt ctttagtgat ttttaaaaat cccgtatcac gcagttaaag
     1921 tggcgggcat actcttcaag gctggtgatc cccaaccgca cccatttagg atgtgaccat
     1981 tgcggtagac cgatgtaaat cataacgcgt gaaggatctc ttccacgcta cgcacacggg
     2041 cgatgcgcgg gtagatatga ttaatgctgt tattgtgttg ctcggcgctg gcggcactac
     2101 aggcgtcttc ggcaatcacc agattaaagc caagttccca ggcattgcgg gcggtggatt
     2161 caacaccgat attggtcgag atcccacata acactattgt atcgatacct cggcggcgca
     2221 gttgcaactc cagatccgta ccgtaaaacg ccccccactg acgtttgatg atttcgatat
     2281 cactgtcagt tgcacctaat gcagctggat gttgccacca gttttcgggc aacactttag
     2341 cgggggagtg cccctattaa gttgtcaagc atgttatgac ccctgcgggg tataaaaagt
     2401 cccgtcgcgc atcatggcga acagaacgtc gcagcgtcgt ctcgccaggg cgataagc
//
LOCUS       DAEEGA010000232         2392 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEEGA010000232 DAEEGA010000000
VERSION     DAEEGA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.237"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..109
                     /locus_tag="KLH46_004256"
     CDS             <1..109
                     /locus_tag="KLH46_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBE0380669.1"
                     /translation="LQTHLDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            102..1061
                     /gene="ssuA"
                     /locus_tag="KLH46_004257"
     CDS             102..1061
                     /gene="ssuA"
                     /locus_tag="KLH46_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBE0380670.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1058..2203
                     /gene="ssuD"
                     /locus_tag="KLH46_004258"
     CDS             1058..2203
                     /gene="ssuD"
                     /locus_tag="KLH46_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBE0380671.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2212..>2392
                     /locus_tag="KLH46_004259"
     CDS             2212..>2392
                     /locus_tag="KLH46_004259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HBE0380672.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          527 a          654 c          673 g          538 t
ORIGIN      
        1 tctgcaaacc catcttgata ccgcgctaga aactttctgg caggcattgc accgccgcga
       61 tgttcaggtt cctgaccttc tgtctctgcg aggtaatgcc catgcgtaac atcattaaac
      121 tggcgctggc gggattgctt agcgtctcta cttttgcggt tgctgcagaa tcctcgcctg
      181 aagcgttacg tataggctat cagaaaggca gtattggtat ggtgctggca aaaagccacc
      241 agttactgga aaaacgctat ccgcaatcaa aaatctcctg ggtggagttc cccgcgggtc
      301 cgcaaatgtt ggaagcgtta aacgttggca gtattgatct cggcagtacc ggggatattc
      361 cgccaatctt tgcccaggct gccggggctg atttggtata tgtgggcgtc gagccaccga
      421 agcccaaagc cgaagtgatt ctggtggcag aaaacagccc gatcaaaacc gtagccgatc
      481 ttaaaggtca caaagttgcc tttcagaaag gttccagttc acacaacctt ttactgcgtg
      541 cactgcgtca ggccggactt aaattcaccg atatccagcc cacttacctg acgcctgccg
      601 atgcccgcgc cgcgttccag caaggtaacg ttgacgcctg ggctatctgg gatccctact
      661 actccgctgc gttattacag ggcggcgtgc gggtgctgaa agacggcacc gatctcaatc
      721 aaactggatc gttttatctg gcagctcgtc cctatgcaga aaaaaacggc gcttttattc
      781 agggcgtact ggcaaccttt agtgaggccg atgcgttaac ccgcagccag cgcgaacaaa
      841 gcatcgcttt actggcaaaa acgatgggct taccggcacc ggtgattgcc tcttacttag
      901 atcatcgccc tcctaccacc atcaaaccgg ttaacgccga ggttgccgcc ttacagcagc
      961 aaacggcaga tctgttttat gaaaaccgtc tggtgccgaa aaaagtcgat attcgccagc
     1021 gcatctggca gcccactcaa ctggaaggaa aacaattatg agtctgaata tgttctggtt
     1081 tttaccgacc cacggtgacg ggcactatct gggaacggaa gaaggttcac gcccggttga
     1141 tcacggttat ctgcaacaaa ttgcgcaagc ggcggatcgt ctgggctata ccggtgtgct
     1201 gatcccaacg gggcgatcgt gtgaagatgc gtggctggtt gcagcgtcga tgatcccggt
     1261 gacgcagcgg ctgaagtttc ttgtcgccct gcgtcccagc gtaacctcac ctactgttgc
     1321 cgcccgccag gccgccacgc ttgaccgtct ctcaaatggt cgtgcgttgt ttaatctggt
     1381 cacaggcagc gatccacaag agctggcagg cgacggcgtg ttccttgatc atagcgagcg
     1441 ctacgaagcc tcggcggaat ttacccaggt ctggcggcgc ttattgctgg gggaaaccgt
     1501 caatttcaac ggtaaacata ttcatgtgcg cggagcaaaa ctgctcttcc cgccgattca
     1561 acagccttat cctccgcttt actttggcgg atcgtcagat gtcgcccagg agctggcggc
     1621 agaacaagtt gatctctacc tcacctgggg cgaaccgccg gaacttgtta aagagaaaat
     1681 cgaacaagtg cgggcgaaag ctgccgcgta tgggcgcaaa attcgtttcg gtattcgtct
     1741 gcatgtgatt gttcgtgaaa ctaacgacga agcgtggcag gccgccgagc ggttaatctc
     1801 gcatcttgat gatgagacta tcgccaaagc acaagccgca ttcgcccgga cggattccgt
     1861 aggacaacag cgaatggcgg cgctacataa cggcaagcgc gacaatctgg agatcagccc
     1921 caatttatgg gcgggcgttg gcttagtgcg cggcggtgcc ggaacggcgc tggtgggcga
     1981 tggtcctacg gtcgccgcgc gaatcaacga atacgccgcg cttggcatcg acagctttgt
     2041 gctttcgggc tacccgcatc tggaagaagc gtatcgggtt ggcgagttgc tgttcccaca
     2101 tctggatgtc gccattccgg aaattcccca gccgcagccg ctgaatccgc aaggcgaagc
     2161 ggtggcaaat gattttatcc cccgtaaagt cgcgcaaagc taaggaacac gatgatggca
     2221 acgccagtga agaagtggtt attgcgcgtt gccccctggt ttttaccggt gggcatcgtg
     2281 gcggtgtggc aactggcctc ctcggttggc tggctgtcga cgcgtatttt gccttcaccg
     2341 gaaggagtgg tgacggcgtt ctggacgctc tccgccagcg gcgaactgtg gc
//
LOCUS       DAEEGA010000233         2358 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEEGA010000233 DAEEGA010000000
VERSION     DAEEGA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.238"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(419..1873)
                     /gene="ospC4"
                     /locus_tag="KLH46_004260"
     CDS             complement(419..1873)
                     /gene="ospC4"
                     /locus_tag="KLH46_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HBE0380673.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSK
                     FNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKVN
                     SGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          684 a          440 c          408 g          826 t
ORIGIN      
        1 gatcgaaagc tgattttcag gaaccgtttt gacggcttcg gctttaaact ccgtagagta
       61 acgttgggtt tttctgctca ttattggctc cttctgatgc cattctattt caggaaggag
      121 tgtccgttaa actcaggcta cctcactccc gtaaagacag gagtcaagtt cccgctccga
      181 cataactgcg tgcaatggat tttgaaggaa ccgtcagccc attccccgga ggcaaacatg
      241 ggctggcaaa agtgtagcgg tattaggcac agctatttag gaaagaggga ccatctccat
      301 ctcattaaat ggaccacgcc agacaattca ctgtcatgct gatgctgcac tctggggaca
      361 gaatcactca tgatgacttc gataatcgac gacattatta tttggccgag cttttaggtc
      421 atatctcaaa tcgtttgcct aatattgctc cattttttaa taaaaaatca atcatttttg
      481 aatctttgct tttcattgca ttgtcaagca tagtgtcccc actatttact ttttgaaatt
      541 ttttgtttac atcaaccaat ccattattga taaaatattc tagcacttta tagttggcac
      601 catctttact aagtaagtat tcaacatcat acagattaca atatctattg tttttcattt
      661 tctccatctc tgcgacatct tgttttgtaa aattaaattt agatagtaaa taaagtgcta
      721 tatcagcttt tttattgcct aaagaataag caagggcctg atgagccatt tttttatcag
      781 tcactttatt gttaatctct tcataattag atgctttaat cgcatcctcc aaagatatgt
      841 ctctgagttt tatttttttg aagttatctg tacttagcat tcttggtata tgatactcaa
      901 gctgaaacac aaagtttata attctatcca gactaacagg atcaatattt ttgttataac
      961 aaaactctct gaatccttga tctttactct ttcttataaa atcaataaga tgcaacccaa
     1021 ttccaagcct catatcttta gtgttaaata ttggaacatc agttttcccc tgattaccat
     1081 gcacatacct gctaacttca tccacaacct ctttaaagtt atctaagaaa aatgattggt
     1141 gctcatggta aaaaacaggt ggaatagcat tatcaaagtg atcggttaat gtcatatatc
     1201 catatggaga gtcatgatca atgatatacg cattggcgcc aaaatctaca gtatgatgtg
     1261 ttttatttag tgggagtttt tcttgatgat tggaaatttc gaccccaaaa aaaacaaaat
     1321 cgtggttaga gatttcttta atatcctcag agtatgtatt cttatgtgtt tgtatatttt
     1381 cagactgaat tttatcaaag gatttaatat taagaatatt atttgacaga ttagagttac
     1441 tttgatgtct gaagttaaaa ttagaattaa taataataga aagtaagtga cgttcgtctg
     1501 aagaaagagg atgaactttt tgtattatct cagagatata gtgcctggac accttatcga
     1561 gatgctcaag tgaccgaaat gatgaccgtg ctccagatgg cgcttcaata gaaagattta
     1621 gtgatttgaa actggtccct tgagaaaaca ttttgctata actttgcgct gctatagact
     1681 tccttaaaaa gtttttcaca gtattagcac agtgatctaa atgtttttca cttatgccag
     1741 aagaagatga gtttgttacg cgtaatacat ttttttgtaa tgcttttgtt gctttattcg
     1801 gattcgtttt tccatcaaca agatcaatat tattctgaac attaatatga tttactgctt
     1861 caggtatttt catccccccc cccaattatg tgcgttttta tctgtggtta tttagataac
     1921 attatctaac tggacacttt cttgtatgta ttatagctaa atgaacagat tttaatatgt
     1981 tgtatggttt tcactctgaa atcccctgaa tttaatcacg tttcctttac tgaaggcaat
     2041 tttaaccgta gtatcaatta ttaacccttg ggcttgatat ccctctcctc ttctgggcac
     2101 gggggatgtc agattactta ttacagaaac caaaagaatt gtaagcgtca tctcagtgtc
     2161 gtctatttca atgtctgatg attggcgatg atagagtcct cttataacgt taatggactc
     2221 tatcaatgtc agattctctt cgcccccgca ggattcgtgc ctcttactct atgactctat
     2281 ggagtttaag cttgatctta ttgaaaaatc ttaccagcta ggggcctgcg tggcccaact
     2341 ggccagagag tacgggat
//
LOCUS       DAEEGA010000234         2283 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEEGA010000234 DAEEGA010000000
VERSION     DAEEGA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.239"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(485..877)
                     /locus_tag="KLH46_004261"
     CDS             complement(485..877)
                     /locus_tag="KLH46_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380674.1"
                     /translation="MARPVQSHDNTEVRQYLIDKLQMDYGFRYELPGWLDDVLTGAGR
                     LNGSLGSNTKPVSKYVILTQLMSHDVITVEAVRTSISRKKMALEGKMISERYARHVAS
                     VVAAASKSIEFYKLRSKTHWKDEIALAA"
     gene            complement(1355..1918)
                     /locus_tag="KLH46_004262"
     CDS             complement(1355..1918)
                     /locus_tag="KLH46_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE0380675.1"
                     /translation="MRFGYVRVSSQDQNTERQEAALKPHCDEILIEMKSGKDIKRNVL
                     ESLLARLRAGDELVVKSIDRLARNTKDLLTLMDELVGRGVTVRFLDNAMSFDNSPTSR
                     FMLTMMGAVAELERGFINQRQREGIAIAKEKGVYQGKPKNAELRKTLAKLFARGMSAE
                     EAAKVAKCGRATAFRIKKELSEASSGK"
BASE COUNT          559 a          578 c          567 g          579 t
ORIGIN      
        1 aaaaagggcg aacccctgat gcatctattc gacacgtcag gggttctata gtaagttgct
       61 tagcgttacg gctaggcttc tcaccgattg gcgtcattga gaataaccta ggttccgcgc
      121 cgagtccaac gggaacagaa gaaccagcca ttcggactat aaattaccgt aaccagtgag
      181 cgcccttgat gcggggtcct tagtgataaa caggcaagaa gtggtgacgt aacttggccg
      241 cgaaagagtc aagctctcac cgggaaccag accgcccgag ttaaagggtt tacctgataa
      301 gctccagatt aagacgccaa ccacaaggat gcctgccggc ctgtctctag gtcaccacta
      361 cggcatccaa ggacccttat tgccgtaaat tcactctccg tatgcttgct ggtggccccc
      421 tctggtgaat cccgaggggc aatctccttg ctggtcatcc agatttcaca cagcgcgttc
      481 atacctaagc agccagtgct atttcatcct tccaatgtgt cttacttctg agcttgtaga
      541 actcgatact cttgcttgcc gctgcgacca ccgaagctac gtgtcgggca tacctttctg
      601 aaatcatctt gccttcaagg gccatcttct tgcggcttat ggaagtcctg acggcttcca
      661 ctgtgatgac atcatgggac atcaactggg tcaggataac gtacttggat accggcttag
      721 tgttgctgcc aaggcttcca ttgaggcgtc cagcgcctgt gagtacatca tcgagccagc
      781 ccggaagctc atagcggaag ccgtagtcca tttggagttt atcgattagg tactgtcgta
      841 cttcggtatt gtcatgtgat tgaactggtc gtgccatcag gaacctcctt gaatatgctg
      901 tacgttgctg aatagggtgg cgaaatggca gacccagcaa gcaggcgcag ggagaccagt
      961 gattacggaa gtagatataa gggacctgta ggaacaggag aacctggagt tacggaagta
     1021 gatataagag aactcattga gcaactttgg ggggatggat gttgatgaca attacccatc
     1081 caactttgag aacaacagta ggccaatacg gttctcttaa agttattatt taagtagtaa
     1141 tatcaataat taatacaata ataagaacta actttaagtt tatctttaag tatggtcgct
     1201 tcgctccctg ctcgtcacgc tctccctata gtgagttgaa ttgcttgacc tgccgattta
     1261 aaagtaaaac ctgagttttc tcaaggtcct gaaaacggtc aacagataag caaacacaga
     1321 tgctcccagc ccgatggtaa ttggctaccg gtcatcactt cccgctggaa gcctcactga
     1381 gttccttctt gatgcggaaa gctgtcgccc ggccacactt ggcaacctta gctgcttcct
     1441 cagcggacat cccacgagca aacagcttag ccagcgtctt gcgcagctca gcgttcttcg
     1501 gtttcccttg gtagacaccc ttttctttgg caatggcaat gccttctctc tgtcgctgat
     1561 tgatgaagcc acgctccagt tcagccactg cacccatcat ggtcagcata aagcgactgg
     1621 tgggcgaatt gtcgaagctc atggcgttat cgaggaatct cacggttacc ccacggccta
     1681 ccagttcatc catgagggtc agcaggtcct tcgtgttcct ggctaacctg tcgatacttt
     1741 tgaccaccag ctcatcacca gccctcagac gcgctaggag cgattctaag acgttcctct
     1801 tgatatcctt accagacttc atctcaatca atatctcgtc acagtgtggc tttaaggccg
     1861 cttcctgacg ctctgtgttc tggtcctgcg aggacacgcg gacataaccg aatcgcatga
     1921 ggtattttcc tgcttacttg tatcattaag gtggctctca agatacagtc tcaataggct
     1981 cagtgtcaac gtcataatga tactcataaa cgtggagcct ccttggtatc tttggagtgt
     2041 actcaatgga tactgagctg gtgggccaat ggcaggccgg atgccgtacg cacagaaacg
     2101 cagtcagatg ttctctgcga ggctgagggg tatcaataat agggatgtac gggacccgcc
     2161 aagataacgc gacaggggcc ttctggtgaa gcctcagagg gtcgctgggg gaacctgagc
     2221 ggagttaatt agtgatatga ggtcagacaa atttgtggtg gatttctaaa ggctccggga
     2281 ccc
//
LOCUS       DAEEGA010000235         2236 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEEGA010000235 DAEEGA010000000
VERSION     DAEEGA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.240"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            53..2236
                     /locus_tag="KLH46_004263"
                     /pseudo
     CDS             53..2236
                     /locus_tag="KLH46_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          599 a          497 c          562 g          578 t
ORIGIN      
        1 cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac
       61 gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat
      121 ggccgaggtc agtgtattca atccggcgct tctggaaatc aaccatcaat ccggagtcga
      181 tattcgccag tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt
      241 tatcaacggt aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc
      301 tgatctgcac gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt
      361 tgatgcgctc aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg
      421 tattgaaggc tcatcctggc agtttgacag tgataaattg cagctgaata tatccattca
      481 ccaaatctac atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg
      541 gattaatgcg ctcaccatca actacgattt ttctggttca catacactac gttcagatta
      601 tggttcacaa gagacagata ccagttatct caatctgcgc aatggactga atattggacc
      661 gtggcggcta cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc
      721 cattagtacc tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga
      781 tacgtggacg gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac
      841 tgataacgat atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc
      901 aaagtccaac gccaccgtca tcattcggca gaatggctac gtgatttatc agtcagccgt
      961 tccacaaggt gcttttgaga tcaccgatct caacaccgca agtacaggtg gcgatttgga
     1021 cgtaaccatc aaagaagaag acggtagcga acaacgattc acccaacctt atgcttcatt
     1081 ggcgattctt aaacgtgaag gtctgacaga tgttgatgtc agcgtgggtg aattgcgcga
     1141 tgaagacgga tttacaccgg acgtccttca ggcgcaaata cttcatggtt tttcccacgg
     1201 gatcacttta tatggaggta tgcaggctgc tgaaaattat ggttctgcag ctctgggtgt
     1261 cggtaaagat cttggcgctt tgggcgcaat ttctttcgat gtgacacatg ctcgtgcgaa
     1321 ttttagccat gatgatacag aaacgggtca gtcatatcgc tttctctatt caaaactatt
     1381 tgacgacaca gacactagct tgcgcctggt tggctatcgt tactccaccg agggctacta
     1441 taccactcaa tgagtgggca tcgcggcgca acagccctga agacttttgg gaaacaggta
     1501 accgacgtag tcgcgtggag ggaacgctaa cgcagtcgtt ggggagagat tatggcaatt
     1561 tatacctgac attaagccgg caacaatact ggcataccga tgatgtcgaa cgattaatgc
     1621 aatttggcta cagcagtagc tggaagcgtc tctcgtggaa cgtctcctgg agttattcca
     1681 atactgccag acaggggacg gggaacaacc atgccagtga taacaccagt gagcagatct
     1741 acatgctctc tttatctgtt cctttatcgg gctggtgggg taatagttac gccacctatt
     1801 ctgtttcgca aaacgataat tccggtagct cacatcaact cggactcagc ggtacggcgc
     1861 tggaaagaaa taacctttca tggaatttaa tgcagtccta taacagtcat gatgatgagg
     1921 ttggcggtaa tatgtccctg acctatgatg gctcttatgg cacggtgaac ggcagctata
     1981 actacagcca aaattcccag aggctgaatt atggtatcag agggggaatt ctggcacaca
     2041 gcgaaggggt aacgttaagt caggagttag gtgaaactat tgctcttgtt aaagcacctg
     2101 gggccgccgg gttagaaata gataatatgc gcggtgctgc gacggactgg cgcggctata
     2161 cggtcaagac acagctaaac ccttatgatg aaaatcgggt agcaatcagc gataactatt
     2221 tctcgaagtc gaatat
//
LOCUS       DAEEGA010000236         2213 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEEGA010000236 DAEEGA010000000
VERSION     DAEEGA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.241"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(53..322)
                     /locus_tag="KLH46_004264"
     CDS             complement(53..322)
                     /locus_tag="KLH46_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBE0380676.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            379..1047
                     /locus_tag="KLH46_004265"
     CDS             379..1047
                     /locus_tag="KLH46_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE0380677.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1363..>2213
                     /locus_tag="KLH46_004266"
     CDS             1363..>2213
                     /locus_tag="KLH46_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBE0380678.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIW
                     HAFEHEE"
BASE COUNT          666 a          497 c          416 g          634 t
ORIGIN      
        1 gccaaccgca gcacgtcctg catacgtcgt gtctgcggtt tttctttttc gcttacattg
       61 tgtctggttc ttccggccac tcaatatcag gtgcagttga tgtatcaaca cggttcagca
      121 acacccgata cttcttccag gcttccagca acgagttttc ttcctccgtt gcgatctcca
      181 gatctacagc atcctgaagt ggcgcaatat gctcactgaa tccctggatg tagaactgtg
      241 tggtgacggt cttccagcca ttcggctcct gctgtatcga agcataccag gctatttcaa
      301 tatcgctatg ctgcggcagc atttaacccc ttgtaattca tcgccataat tgatttaatt
      361 cacaaataaa actataatat ggtgaaatta atgaaaaaaa acacagatga tggggctaaa
      421 atttacacac cacttaccct aaagctttat gactggtggg ttttgggagt atcaaatcgg
      481 cttgcatggg gatgtcctac aaaggaacac cttcttccac actttctgga acatttaggt
      541 aacaaccatc tggatattgg tgttggaact gggttttacc ttactcacgt acctgagagt
      601 agtctgatat ctttaatgga tttgaacgaa gctagcctga acgcggcatc tacaagggct
      661 ggggaatcaa aaattaaaca taaaattagc catgatgttt ttgaacctta tcctgcggcg
      721 ttacatggtc aatttgattc catttccatg ttttaccttc ttcactgcct gcctggaaat
      781 atatctacaa aaagctgtgt aatacgcaat gcggcgcagg ccttaactga tgatggaact
      841 ctatacggag ccacaattct tggcgatgga gttgtgcaca atagcttcgg tcaaaaactg
      901 atgcgcattt acaatcagaa aggcatcttt tcaaacacaa aagattccga agaaggctta
      961 acacatatac tctcagagca tttcgagaat gttaaaacca aggttcaagg tactgtagta
     1021 atgttttccg cttcagggaa aaaatagcat ccaaccgcag cacgttcttg cttaagacgt
     1081 gctacggttt cctttacgat ttggggttgc gactttacct ctattgataa tgcattccgg
     1141 cagaacgttc aaatatgaac gtacgatatt taactaaccg aaaaacaaaa tatagcacag
     1201 gcgagacatt taccatctaa gaaaatagta tcgttttttt acagttaaat caacatcact
     1261 tccttaaaat gaaaacaata aatttcaatc ctgaaacatt ttatttcagc ttattattcc
     1321 ccagtatcac attttatcag gatatccttc tgcgaggtta ttatgcttcc tgtaaataat
     1381 ccccccctat ccactggaaa cgtctctttt tacagaacta catcaatcga caatgttcac
     1441 aataattatc tctccgaatg ggttgaatgg actaaaaaca gcatttccgg agaaaacagg
     1501 gaaactgctt ttacccggct ccaattatgt ctggagaaca gtgaaacatc gttggactta
     1561 tcttgtttag gtctcagatc tctaccacga ttgcctgaca atcttgatga aattaatgta
     1621 agcaataacc aactatcaat gctccccgag ctaccaaggg cattgaaaga gctgaatgca
     1681 agcagtaatc aattatctgc acttcctgaa ttaccagtgt cgctggaata tataaatgtg
     1741 agtgataacc atttgttcgc acttcctgaa ttacctgcgt cactagaata tattaatgta
     1801 agtgacaatc acctgtctgt acttccgagg ttaccaatgt cattggaatt acttgatgca
     1861 gccagaaatg ctttggaagt aataccagat tttccagaaa gagatgatca tattataaga
     1921 atattctggc ttaatcagaa ccggatcacg gcaattccgg aaagcatact tggcctcagt
     1981 tctgatagcg ttgtcaatct tagagaaaat caactatctc ccagaataat gcaaactttg
     2041 ttacaacaaa ccgcccaacc ggactaccac ggcccacgga tttacttctc catgagtgac
     2101 ggacaacaga atacactcca tcgccccctg gctgatgccg tgacagcatg gttcccggaa
     2161 aacaaacaat ctgatgtatc acagatatgg catgcttttg aacatgaaga gca
//
LOCUS       DAEEGA010000237         2187 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEEGA010000237 DAEEGA010000000
VERSION     DAEEGA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.242"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..698
                     /locus_tag="KLH46_004267"
     CDS             <1..698
                     /locus_tag="KLH46_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380679.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAPPGIDMNGN
                     TTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAFN
                     GTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPV
                     LDVSDISVKLQEA"
     gene            700..1305
                     /locus_tag="KLH46_004268"
     CDS             700..1305
                     /locus_tag="KLH46_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HBE0380680.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSCWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1292..>2187
                     /locus_tag="KLH46_004269"
     CDS             1292..>2187
                     /locus_tag="KLH46_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0380681.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          532 a          507 c          610 g          538 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtcagtat ccattaacac ccggttcgct ttatgttggc gtgtacggcg
      241 gcagcccggc agatatcgcg gcggccatct ggcgtaaggc tccgccgggt attgatatga
      301 atggcaacac aacgttcact gttgcagata aggagtacga tccgccgtat cctgaatacg
      361 tgatcagctg gcagacactc aaacccgtca gtctgcatgt cagtgtgacg ctgaaaaaaa
      421 gtgactacct gccttcagat attacccgac aggtacagca atccgtgctg gacgcattta
      481 atggtacaga tggtggtctg cgggcaagag ttgcctctgt tgtctctgcc gggcgctact
      541 atgccggcat ttacaaaacc gatccggaac atattgatat tctgggcctt actgtgagcc
      601 gtgatggttc gtcatggaca acggctgtca ctttcgggat agatgagatt ccggttctgg
      661 atgtgtcgga tatcagtgtg aaactccagg aggcataacg tgcagaatgt ggctgccact
      721 gtgcttgcgc agtatgctgc cagcccccga ctcaatgccc tcattaacag ctttaacgca
      781 gcgctttccc ccgacagttt tatcagtgat ttttatggtc ttatctggaa catcgatacc
      841 gcagaaaagt atggtcttga tgtctgggga aagattgtgg gtgtcagttg ctggctgacg
      901 gtaaaggacg attttaatta cctgggcttc agcgagtcca ggatggacac cccggtaatg
      961 gatgatccct gtccgtttaa tcaggcaccg ttttacaacg gaaaatcgga tacccggact
     1021 gttgacctgt ctgatgctgt ataccggcgg ctgatactga tgaaagccat gtcgaacatt
     1081 actgactgtt ccgttccgga tattaacctg atgctgagat ttatgttcgg aaaaaaacgc
     1141 cgggcttatg ttctgaataa tggcgggctg aggatgagtt acgtctttga gtccgcgctc
     1201 tcgttggcag aactggcgat tatccagtcg tcgggtgcac tgccatcccc gccgggtgtt
     1261 tatgtttcag tagttttaaa ggagtcccgt aatgaaggcc agtgataaac cccgccagct
     1321 ggcggtcccc tttgcgagta ccggagataa aaaccgtatc ccggacaagg cgacacagca
     1381 gaccagagag agcggtaatg ctgcgtatga ttcaggtttt cctccggtga ccatgacagc
     1441 ggtctcagcg ggaggtatac cgccacacgg caaggatttt aacggtctga tgtacgatat
     1501 taccgcagca atacggttcg cccaggctgg cggtttgtac acgtataatg ccggttttgc
     1561 gggggccatt ggtggatatg caaaaggagc cattctcgcc ggagtcgcaa caacagcggt
     1621 ctggctgaat accacggacg ataacctgac cgatcctgaa ggctccgaca gtgcgggctg
     1681 ggtaaatctt cttgaggatc cgaaaaggat attcctgcgg cagaagaaca atctgtcaga
     1741 ccttcagaat aaagggacgg cacgggataa tcttcaggtt tacagtaaag agcagtcaga
     1801 tcaacgctat gttcatcggg aaggcgataa aataaccgga gagctgaaaa tccgtggtgt
     1861 taatgcgctg aggattttca acgaagcttt tggcctgatt tttcgtcgtt cggaagagtg
     1921 cctgcacctt attcccacca gtgaaggtca gggggaaaat ggcgatattg gtcccctgcg
     1981 cccgttcacc attaatttgc ggacgggtga aatatccatg tcgcataaag tgtctgttgg
     2041 cggtggttcg caggtcaatg gtgcgctggg tatcggcgtt cagaacgccc tgggggggaa
     2101 ttcaattgtt cttggtgata atgacaccgg atttaaacag aatggagacg gtattctgga
     2161 tgtttatgct aatagtcagc gtgtatt
//
LOCUS       DAEEGA010000238         2168 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEEGA010000238 DAEEGA010000000
VERSION     DAEEGA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.243"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            155..340
                     /locus_tag="KLH46_004270"
                     /pseudo
     CDS             155..340
                     /locus_tag="KLH46_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(440..649)
                     /locus_tag="KLH46_004271"
                     /pseudo
     CDS             complement(440..649)
                     /locus_tag="KLH46_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(699..878)
                     /locus_tag="KLH46_004272"
                     /pseudo
     CDS             complement(699..878)
                     /locus_tag="KLH46_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1402..1686
                     /locus_tag="KLH46_004273"
     CDS             1402..1686
                     /locus_tag="KLH46_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBE0380682.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHPLPVPR"
     gene            1686..1961
                     /locus_tag="KLH46_004274"
     CDS             1686..1961
                     /locus_tag="KLH46_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE0380683.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          601 a          539 c          522 g          506 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaaggg gatatatcgc
      421 agctctgacg actcactcct tatttacccg ggaaatactt ctccagcaat gcatacagga
      481 tggcagcggt ttcagcatcc attatgccgt cataattttt ctgccggaaa tgaagctgaa
      541 aagcgcggac aagcgcctta tatccgtcat ccgtctccac accagaaata ctgtatccat
      601 aggtcttcag ccgtgacacg atatcaacct tatccggaat gccctgcgat tcaaaaaata
      661 aatatttagt cagtaatttt atgtttttcc tgaaaaaatt actctttttc gaactccagt
      721 gagcgttcac gactcatcag tgtgaaaatg atggcaaaaa cagcagacag tatcagtacg
      781 ataaatgtgg ccccccatcc cagataatcg acaacaagtc ccattcccag tgtcgcaaca
      841 attgagccga agatatagga aaatgtgcct gacagcccta acagaataag tttacgcgta
      901 aatcgcccgg aatccagaag agcattaagt atcacataaa caggttccat accagtcctg
      961 accgcatcag gacgccagta aataaaatct cgtagtccac cggcatcaga gacaagagta
     1021 atcgtcgtta ccggtatttc gactggtttg tactggtgtg agcggacaaa actactgtac
     1081 gtctctcata cacgctcgta agcggccctc acggcccgct aacgcggaga tacgccccga
     1141 ctgcgggtaa acccttgtcg ggaccactcc gaccgcgcac agaggcattt tcatcgctga
     1201 caggccgcat ggcctcgcag agccggaggg ggactggtga tattcggacg accgtaccgg
     1261 cttgcgccgg gcttcggcgg ttttacacct gtgctacatg taaccaccgt atgccgcctt
     1321 atatgcgctg actcgcatta tttgccttgt tccgtctgaa agtaatcact atgattaaat
     1381 atgattaaca actaatcggg tatgcaaatg aaaaacaata ccgcacaagc aacaaaagta
     1441 attaccgcgc atgtgccatt acctatggct gataaagtcg accagatggc cgccagactg
     1501 gaacgctccc ggggctgggt tatcaaacag gcgctttctg catggcttgc ccaagaggag
     1561 gagcgtaatc gcctgacgct ggaagccctg gacgatgtga catccggaca ggttatcgac
     1621 catcaggctg tacagtcctg ggcggacagc ctcagtactg accatccgtt accggtgcca
     1681 cgctgatgga actgaagtgg accagtaagg cactttctga tttggcgcgg ttatatgatt
     1741 ttctggtgct ggccagtaaa cctgctgccg ccagaacggt gcagtccctg acacaggcgc
     1801 cggtcattct gttaactcat ccacgtatgg gagaacagtt gtttcagttt gaacccaggg
     1861 aggtcagacg gatttttact ggcgagtacg aaatccgtta cgaacttact ggccagacta
     1921 tttatgtatt gcgcctgtgg cacacacgag agaacaggta gattgggtgt ggaggcaact
     1981 ttaacatagc ctgcaggtat taattctgga gcattgttat caggccagat attttgcatt
     2041 ccattaaata atcttgtagg agataaaata aattacgatg aaataacgaa aattaccgct
     2101 cgtgactggc ggcaatatcg cgctccgggc tggcaaataa cccatcagaa acgttattgc
     2161 cagacatt
//
LOCUS       DAEEGA010000239         2127 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEEGA010000239 DAEEGA010000000
VERSION     DAEEGA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.244"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            1..839
                     /locus_tag="KLH46_004275"
                     /pseudo
     CDS             1..839
                     /locus_tag="KLH46_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            954..1295
                     /locus_tag="KLH46_004276"
                     /pseudo
     CDS             954..1295
                     /locus_tag="KLH46_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1278..2115
                     /locus_tag="KLH46_004277"
                     /pseudo
     CDS             1278..2115
                     /locus_tag="KLH46_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          527 a          515 c          640 g          445 t
ORIGIN      
        1 gaacaaatgg tagtgtccgc tattgccagt acacctcagt tctgggcctt gcccgtagca
       61 gttattttta tcaccgggct gcactgaaag ccggtgataa atacgccacg atacgtaaga
      121 tgctgacaga tatctttaac agtaattacc agtgttatgg ctatcgtcgc ctgcatgcga
      181 tgctcaggca tgaggggggg cggctatcag aaaaggttgt acgcagactt atggtggagg
      241 aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag ctcatattgc ggagaaatcg
      301 gaccggctcc ggataacctt atcgccagag attttaaggc ggagcaacct aatcagaaat
      361 agctgacaga tatcacggag ttccagctcc ctgcaggtaa agtctggctg tcaccagtgg
      421 tggactgctt cgatggaaaa gttgtgagct ggtctctcag tacacgccct gatgctgaac
      481 tggtcaacac tatgctggat agcgctgtcg aaacgttaaa tgctggcgaa cgaccggtga
      541 tacacagtga cagaggtggg cattatcgct ggccaggctg gctggaaaga gtgaatgcag
      601 caggtcttat tcgctcaatg tcccgtaaag gatgttcacc tgataatgct gcatgcgaag
      661 gctttttcgg cagactgaaa acggaaatgt attatgggcg taaatggtcg ggcatcacgc
      721 cagaaaagtt catgcagcaa gtggatgctt acatcagatg gtataacgag cggcgtataa
      781 aattatcgct gggtgccgtc agccccaaaa tgtaccgcca acaatgcggg ctggaatgat
      841 aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat ttggattgtc cgctccaccc
      901 aacatgttgt ttccttaagg ttcctacacc acaaaggaaa ctctcatgct gcgcagtgga
      961 tggctgacgt tgccgacagg cgggaacttc gccagttcag gcagacaccg gaacagcgct
     1021 tcacgcagga acaggagcat ctgcagccgc tgctgggtac ggacttcgat atccgtcatg
     1081 tctcctggga tggctatatc gaggttggcg ggaaccgtta cagtgtgccg gaaagcctgt
     1141 gtggtcagct ggtatcaata cggatctcgc tggatgatga actgcggatc tacagtaatg
     1201 agcaacaggt agcatcgcac cgtctctgtt cggctgcata cggctggcag accgtgccgg
     1261 agcatcacgc tccactctgg cagcaggctt cggagcgcat ggccgaatgg ctgggggaga
     1321 tacagaaacg ggtaatcact gtctgtgacc gggaagcgga tatctggcat tacctgtatt
     1381 ataaagtcag ccacggacag cgtggtgcgt gctgcacaga atcgccggct ggaagaggca
     1441 cccggcaagc tcttcgaact gccggaagtc ctggcaaccg caggaagcca cacgctgaat
     1501 gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga tgttcatcag ttacagcgaa
     1561 gtcagcataa aaaatcccga caacagcggc caggcgctcc cgctcacgta tgtctgttgc
     1621 cgggagcagg cagaggacgg tgcctgctgg catctgctga cgtcaggaaa agcggcgagt
     1681 gccgccgatg cacgacgtat tgtcagccat tacgagcgac gctggctgac agaggaatac
     1741 cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg aatgcagacc cgggataacc
     1801 ttgagcgcat ggtggttatc caggcgttta tagcggtacg ggtgctgggc ctgcggcagg
     1861 gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa aatactgacg ccgacagagt
     1921 ggaaactact gtgggttaag ctggaaggaa agccgcttcc ggtgcaggca ccgaccctga
     1981 aatgggcctg gggcgatggc atgacagcaa acgcacaggt cgtcccggtt ggggcgtcat
     2041 gtgggatggc tggtcagact tcaggatatg gttgagggtt acctggtgat gaagtctctt
     2101 gatcaggagc tctgatcaag agacagc
//
LOCUS       DAEEGA010000240         2126 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEEGA010000240 DAEEGA010000000
VERSION     DAEEGA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.245"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            43..222
                     /locus_tag="KLH46_004278"
     CDS             43..222
                     /locus_tag="KLH46_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380684.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            274..468
                     /locus_tag="KLH46_004279"
     CDS             274..468
                     /locus_tag="KLH46_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380685.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1267..1603)
                     /locus_tag="KLH46_004280"
                     /pseudo
     CDS             complement(1267..1603)
                     /locus_tag="KLH46_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          682 a          437 c          436 g          571 t
ORIGIN      
        1 tagcgtaagg cactacagcg cattgcttta cgagtaataa caatggattt atcaccagaa
       61 gacgcaaagt ttttacgaga actgcaacac cgtatgctca gagaagccat tatcgaacag
      121 caaacgggca tgagcgccat agacccattg cgatacaaac gggcctcatc tcctttagtt
      181 gacgacaatt ccaatgcgga taaaacacgt ccaccacact gattacattt ttcattagca
      241 cagtggtgtg atgcagcata cgaggaaaac cggatgaatg agaacaaaat aaaaaggctg
      301 gagcaactgt tacaggcacg ccaacgggag tttgccacta aatggggacg tgccgcgctg
      361 ccacatgaac gcctaaccta ttttccactt gaagacctca ttaaaaataa tggcaaaaaa
      421 atcaggtctg ataagaaaca cttcttagaa tgcaatgaag acaaataaca acaatctgca
      481 tattgccacc aaccacagat gtggcatcgt tatcagcgca cttgattttc tttatattca
      541 ctgacgagct tgccggggca cctttgtttg ccggtcattc tggtatcatg cagatattta
      601 cggcgtacaa gcccactagc atgacattgt aataaggcct gagcctaacg ctttaatgtg
      661 tgtcatacac tcagtggcgt aatcgttgac aatatggtgc atgtaatcgt aagcgtccag
      721 cgaaatccat ctggtcataa tctgaagcat ccgacaaagt ggtgtccacc aaataagtga
      781 tctgcgccca atatcctgag ccggaacttt gacaaaggcc aacgaaaaat gggagcggat
      841 gttacagagt tctcggtaca ggggaaaagt ggtacctgtc gccagttctc cgatctgttt
      901 aaccgggaaa ttatctccta cagcttgtca gaaaggtcgg tgatggagca tcgttaatac
      961 gatgctatac gatgcattca cagtgcccgg ccagaggatg ccccgtcgct gcatatggat
     1021 cagggttggc aatatcgaat cgcaggctat aggcaaagtt aaagccccat ggagtagcac
     1081 aaaatatgcc gcacaaagga aacggtctga ataacgcagt gatgaagaac ttcttcagca
     1141 cactgctaaa acgcatagtg atcgagcgct gattctggcg aacaactgaa ctaatacatc
     1201 agaatatgca ttatgttaaa taaatatgaa aagatggttt aaataccccg ttacttttga
     1261 cttacactat acggtatcgc atcgtttaat attcgcaccg gccagatttt tatttctatt
     1321 agttgtcaca atactgaatg cgtacgacca cagtattctg gctcctgtgt ggttatgctt
     1381 taattctgcg ttccgggcag ataagcagtt gcttgcagga atccttcttg tgttaatgtc
     1441 agttcccctt tttaccagtg ctgatttcca cattccgtcc aacagagctt atagcctttc
     1501 cctggattat agcattgtcc ggctgaagtt ctttttgaat aataatagaa gcactgctgg
     1561 cagatccagt ccgtttttca taacccactg tactgataac cataatctaa tcagtagaaa
     1621 ttgagtcgaa aataagcact actccataca ggataattag aggtcagttt gattattcac
     1681 aattcatcat cagcattttc tatttctgac gaaatcaaca tgaaaataac catatatgat
     1741 aattattata ataacggctt taattggaat acatatatta caacgtatta tatataattg
     1801 gtattctggg aactatattc tcaaaatata gtagaaacga ggtatgtttc tggtggaaag
     1861 gacagtggga ttaaaaagta agaattgata aaaaaaacgc cagcaacaca tagtgccggc
     1921 ggagggaata ccccatggag aaaatgtgat gcctagagca tcatgaatat ataattaaaa
     1981 atagttagcg ttgtcaatac tttcacaaaa ataattttcg tagtatagaa agatattttt
     2041 atgcatgacc tacctgaatt tgctccgggt agaggttata aataaaaatt gaatcacgac
     2101 aaacacaata ttcatagtat ggcgat
//
LOCUS       DAEEGA010000241         2115 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEEGA010000241 DAEEGA010000000
VERSION     DAEEGA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.246"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(137..604)
                     /locus_tag="KLH46_004281"
     CDS             complement(137..604)
                     /locus_tag="KLH46_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBE0380686.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKTNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(670..909)
                     /locus_tag="KLH46_004282"
     CDS             complement(670..909)
                     /locus_tag="KLH46_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HBE0380687.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1044..1649)
                     /locus_tag="KLH46_004283"
     CDS             complement(1044..1649)
                     /locus_tag="KLH46_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE0380688.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
     gene            complement(1764..>2115)
                     /locus_tag="KLH46_004284"
     CDS             complement(1764..>2115)
                     /locus_tag="KLH46_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0380689.1"
                     /translation="AKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGCPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKLVELH
                     DKVIGHYLNIKHYQ"
BASE COUNT          651 a          490 c          391 g          583 t
ORIGIN      
        1 ccggaacaat ttgaaaacca gaaccttgct taggcctgtg tccatattac gtgggtagga
       61 tcacaagacc tactgctatc acaattactg agagaaaaaa aagcatccga tgattcattt
      121 atgctgtcct aaaaaattaa caaaacaatg ggaaaacagg atatgtaagc agaaaagcca
      181 atgaaattgg tacagcataa ggcacaccgt tttcacgaat tgattttcga aaaaaaagaa
      241 gacctgccag aacaaccaga gcgccgcaaa aggtcattgc gacgagaaaa ttagccagtt
      301 catgttcatc aatagctaac gaaagaacag ttaacagctt gacatcaccg ccgccaatta
      361 tccctgcgga aaaaagaaag aagcccacga gcaaaacgct cccggctgca accagattgg
      421 ttttttgatg ctgtaaaatg agtgccaaaa aaataagcac ggttaacacc agtaaataac
      481 gatggctaat aattctgagt aatatatccc cccatgaaat aaaaataaga aagagcgcaa
      541 caaataaaag atcgactctt agtaaccata ttgctgccat tgaaagactc catgtaccat
      601 acaaggttgc tgccagacat attcctttac gtctgacagc ctctagagag tttttttatt
      661 ttttataagt tacggattac tcgttccgga aatcgttacc tgttgaattg ctgcagcgat
      721 agcgtcgaat gcatctttaa ttgcgcccag gaagcctgag ttctgatcgc caaagatgaa
      781 agcaagcaga gttgccatag caacaccaat caatgcatat tcgattgcag taacaccacg
      841 ttcatccttc gcgaaacgcg caaagcactg tttagtttta ccatagtact tagtaataaa
      901 agtattcatg ttttcctcgt atattattat ttaaattcaa accaccagct tcatctgcgt
      961 gtcaccggta tcccatattt tttggatgtg gctataatta tccattcccg ctatttagtc
     1021 ttcaaaaaaa attacattta atatcattta tcttgattaa gaaatagtaa gactaaaaaa
     1081 acaagcgtcg tcagccagca cactgctaac acggtaagaa cgctaataaa aacactccac
     1141 tgcttcgttc tataccattc aatatttttg tcagtatgtt ttattgggag cagctcttca
     1201 gtagagaatt cctggctgtt atcattctca ggaatgtttg catcagtgtt tacttccctg
     1261 gcctcgctat cgttggtata gctactactg tcttcatcac tgtcacgctc attctcacag
     1321 acatgtttct cgacctttcc cagatatctg acgccttttc gcggaatggt tttaattaga
     1381 gaactcgaca aacctgcgac gtctaatgcc tttctaagca tgtaaatctg cccataataa
     1441 ctgctgtcgc ttaccgaacc tttctgctcc cgccaaacct cattgataat atgttgctta
     1501 tcggattctc cctgtagtaa aaggagtaaa aatcgatagc cgttttcaga cagattgatt
     1561 acattgcctg tactggtggt gatacgtcga gacgctgggt aaaacttaac tccctgccct
     1621 aaagaaaaat aaaaacggtc atcgctcata agtaccgacc cctgttttct tcttcacatg
     1681 actactcatt acaactttgt atgaagtaag atgcaaaatc atattgttta cattaagtta
     1741 agcaattagg taatgactcc aacttactga tagtgtttta tgttcagata atgcccgatg
     1801 accttgtcat gcagctccac caattttgag aacgacagtg acttccgtcc cagccttgcc
     1861 agatgttgtc tcagattcag attatgtcgc tcaatgcgct gagtgtaacg cttgctgata
     1921 acgtgcagct ttcccttcag gcgtgattca tacagcggac agccatctgt catccatacc
     1981 acgacctcaa aggccgacag caggctcaga agacgctcca gtgtggccag agtgcgttca
     2041 ccgaagacgt gcgccacaac cgtcctccgt atcctgtcat acgcgtaaaa cagccagcgc
     2101 tgacgtgatt tagca
//
LOCUS       DAEEGA010000242         2100 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEEGA010000242 DAEEGA010000000
VERSION     DAEEGA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.247"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..715)
                     /gene="ipaH7.8"
                     /locus_tag="KLH46_004285"
     CDS             complement(<1..715)
                     /gene="ipaH7.8"
                     /locus_tag="KLH46_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HBE0380690.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDELEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLST"
     gene            1044..1223
                     /locus_tag="KLH46_004286"
                     /pseudo
     CDS             1044..1223
                     /locus_tag="KLH46_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1240..1931)
                     /locus_tag="KLH46_004287"
     CDS             complement(join(1240..1685,1685..1931))
                     /locus_tag="KLH46_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0380691.1"
                     /translation="MTTVTVHCPRCNSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKLGVKEQIVEMAHNGAGGCYTARTLKIGINTVIRTFKKLSPRRITSAPVAHADVA
                     LICELDEQWRFVGSKAQQHWPWYAYNTKTGGVLAYTCGPRNDETCRELLALLTPFCIG
                     MVTSDDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHE
                     KVIGSFIEKHIF"
BASE COUNT          621 a          386 c          471 g          622 t
ORIGIN      
        1 gagtcgacaa tggattccct gaaatattaa tcctgtaact tgaatctaat tgtgaaaaac
       61 tctccggaag agtggttatc tgattaaaat gaaaataata ttccttttgt ctgatattgt
      121 ttctatcaga aaattgagga aaagtaacca gattattgcg ctgagcacag agaatttcca
      181 gttcatccgg taagtcgggt aatgttgtca gcctgttata ataaacatgt aaaaatttca
      241 atttttcggg aagagccggt aagttatata gcctgttatt ttcaacaaat aatgatgtta
      301 aatggtttgg caaaacagga aggttttcca gttgattaga acgaacactt aaagttgtta
      361 atgactcagg aagttctggt atcacagaaa gcctgttatt atcagcatta agttctttaa
      421 gaaatgcagg cagctttggc aatgatgtta atttattatt ttctacaatc aaagcagaaa
      481 tatgctttgg taattcggga agagacgtca aatttaggtc tgataaattt aatactgctt
      541 cttgattctg aaagcactgg gagagtctgt taaaagcaat gcctcgctct tccccgggag
      601 aagagttctt ttcccattca gtcaggattc tcagataatg ttcattactg gttgagtttg
      661 agttaataga gggggagcaa gaaactgatg agtgtgtatt atttacagag aacataatgc
      721 taggtcaacc ttatatttgt gagaataaat atttaaaatt gtatatttat gcaaatttca
      781 cttaccattt ccgatattaa gttttaatag tttccatttt acacataaca gaagttagtt
      841 gtaatttatt tatggaggtt ttctatggaa ttcttttttt attgtgactg cagtcaataa
      901 taacactgaa ttcaacattc cagtgagcaa ggaaatgtaa tccgagtaag tatatttgtt
      961 ttacatcata ttttagtcaa gctcatttat tcctattata gaatgacaaa ttaaaattac
     1021 tgtaaaaaaa cgatacacat ttaatacgag ggaggccggc agaaaaacgg cttgcggtca
     1081 ggcaggagaa atgtgtgccg cgaatgaagt ccttgtatga ctggataaag ctccagagga
     1141 aaactttgtc aaaccatgct gaaatggtaa aggcgttcaa ctatatcctg aattactgga
     1201 atgcactgaa tgagttttgc cgggtagtac ttccaatcat tagaatatgt gcttttcaat
     1261 gaaggaaccg atgacttttt cgtggatttc cacggaacgc gagaagcaga ttgttttacg
     1321 tgccagccgc ttgatgcggg tgcggagggt ccggttgtta cgtgcaatac gttgcgtgaa
     1381 gattttgccg gtcaggtgtt tctccttcgg cacttctctg gcgtagctgc cccagtcatc
     1441 gctggtcacc atgccgatac agaacggagt caacagggct aacagttccc gacaggtctc
     1501 atcattccgt ggcccgcaag tgtaagccaa cacaccgcca gttttagtgt tgtacgcata
     1561 ccagggccag tgttgttgcg ccttgctgcc aacgaagcgc cactgctcat caagttcaca
     1621 gataagggca acatcagcgt gagcgactgg cgcagatgtt atccgccggg gcgagagctt
     1681 tttaatgtcc gtatgacggt attgatgccg attttcagtg ttctggcggt ataacagccc
     1741 cctgcgccgt tatgtgccat ttcaacgatc tgctccttaa cgccgagttt tctggcttca
     1801 taagagtaag tgagctggaa aacacgttta catgagcgac aacggaaacg ctcatgccgg
     1861 gaacaactac ggccatgtcg ataaacttca tcggaattac agcgcggaca atgaacagta
     1921 acggtagtca tggaaaacct caaataccag tagattacca gaaacttcta tcgattgaag
     1981 acattacctt ttgccgcaat aggtggggta acattggtga aaacgcatta cgccgggtgg
     2041 cagttggaaa aaaattatct tttttcggct ctgatcgtgg tggagaaaat gcggcaataa
//
LOCUS       DAEEGA010000243         2096 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEEGA010000243 DAEEGA010000000
VERSION     DAEEGA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.248"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="KLH46_004288"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="KLH46_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBE0380692.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="KLH46_004289"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="KLH46_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBE0380693.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMLILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          560 g          515 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgctcatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tttcgt
//
LOCUS       DAEEGA010000244         2072 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEEGA010000244 DAEEGA010000000
VERSION     DAEEGA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.249"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..367)
                     /locus_tag="KLH46_004290"
     CDS             complement(<1..367)
                     /locus_tag="KLH46_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBE0380694.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFVYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFE"
     gene            complement(406..693)
                     /locus_tag="KLH46_004291"
     CDS             complement(406..693)
                     /locus_tag="KLH46_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380695.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKKIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(943..1098)
                     /gene="hokD"
                     /locus_tag="KLH46_004292"
     CDS             complement(943..1098)
                     /gene="hokD"
                     /locus_tag="KLH46_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBE0380696.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1326..1991)
                     /locus_tag="KLH46_004293"
     CDS             complement(1326..1991)
                     /locus_tag="KLH46_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBE0380697.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          533 a          533 c          466 g          540 t
ORIGIN      
        1 cctcaaacga ctttagcaat gccgtatcac gggttctgcg tgtcgcaact gtattcagat
       61 attgctctgc cgcttcgctc agagctggcg tatgttcccg gcctactgat tcacacaggt
      121 aatcaacgaa accggacacc agttttcgtt cgcgaggcgt gatcgcccca ccgaccggag
      181 tccagtaaac gaaacccagt tgcaggagtt tgaaaaaacg cttgtggaat gcgtagttac
      241 gcacacgctt aaagtctgcg tgtatccact cacctatttt gatttgatgc agaaaatcgc
      301 aactctccgg cgtcgccggg agaagtaatc cggaagaggt ttgtttgacc agttgtatat
      361 gtgccatcgg ctttctccgg tggcacggtg ttacacagca ggagttcaat cctgctcaag
      421 attgtagatg agtttattct tcagcaaaag cagaaaacca gccttaaaac caatcttttt
      481 caaaacccgt aatgatgtga caaattcgtc ctcacgcaaa ataaaaccgt ccgtcagaag
      541 tccattacaa aaataaaata acacagcacc gctcttcctt tgttgagatt gcaaacatct
      601 aatgcggcaa tggctgacaa tcgctccatt ctcaacgcgc acagcataga ggccattttc
      661 actaaaaatt tcacgcaatt cttcgatttt catcttcaga atccttccag ataaatagct
      721 ctcccctgtt cggggtccat ccctcttctc cctgcgcgct acttaagtga gtcgattcta
      781 tctgcgaagg tgcgcgaatc aaattcaccg gaaataaaca acaaaaaacc cgccgaaacg
      841 ctgagagatc acagcgatgg gcgggttaag tgcgggtgcg ttgaggatgc ctgacacatc
      901 agaggtgggc ggggatggga tcagctcccc acccggtcac tcttacttcc tggattcgta
      961 gtctacgaag acagcgacct ccgtctggcc ggttcggatt cgtacctcac agaggtcttt
     1021 cctcgttacc agtgccgtca ctatgacggt taaacagatg acgatcaggg cgattaacat
     1081 cgccttttgc tgcttcatag cctgcttctc cttgcctttc ggcatgtaag aggctaacct
     1141 acatgtgcaa agcatgaaat tggcctcaga ttaatgttaa gcgtcttgcc ggacgcgtaa
     1201 tgttaactgg ggcttttctc tatctgcctt ttggtgttca tgcctgaagc agatagcctc
     1261 aagcacccgc agtcattcta cttacctcgc caatatgaaa tcaatcagaa aggtgcccca
     1321 taaaatcact ccttctcttc tttaccgtag tggagttgac caattttgat aagagggcgt
     1381 ccctgagatt tgcggtgtag attggtatcg cgcagagaat acacacagcc acaatattcc
     1441 tgctgataga atttttcgcg cttgctgatt tcaatcatac gggacgagcc gccctgcttg
     1501 cgccagttat aatcccagta caccatgccc ggataatgcg cgacggctcg ccgcccacag
     1561 tcgttaacct gctgcatatt tttccagcgt gaaatgccca gtgaactgct gatcacactg
     1621 aaaccatttt cagcggcgta cagcgctgtc cgctcaaaac gcatgtcaaa acacatggtg
     1681 caacggatcc ctcgttcggg ctcccattcc attcctttgg ctcgttcaaa ccagttgtct
     1741 gtgtcgtaat cagcatcaat aaacggcacg ccgtgttgtt cagcaaagcg aatattctca
     1801 tccttacgaa ttaaatactc tttctgagga tgaatgttcg ggttgtagaa aaagatggtg
     1861 tagtcgattc ccgaggcctg aagcgcctcc atcacctcac cggaacatgg agcacagcaa
     1921 gagtgcagta gtagtttgtt tgccccgttt gggagctcca atttgggccg tttgaaatca
     1981 gcaactgtca taaatatgtt tattgaggtc ataaaaatat caaagagtgt agcattagaa
     2041 cggggctatc ggaaacagat gtgtgactgc tc
//
LOCUS       DAEEGA010000245         2070 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEEGA010000245 DAEEGA010000000
VERSION     DAEEGA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.250"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(135..1589)
                     /gene="ospC2"
                     /locus_tag="KLH46_004294"
     CDS             complement(135..1589)
                     /gene="ospC2"
                     /locus_tag="KLH46_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HBE0380698.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
     gene            1993..>2070
                     /locus_tag="KLH46_004295"
     CDS             1993..>2070
                     /locus_tag="KLH46_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0380699.1"
                     /translation="MSIRCPSCSATEGVVRNGKSTAGHQR"
BASE COUNT          642 a          367 c          339 g          722 t
ORIGIN      
        1 tctccaggat tcccggggcg ttcaccacat gcaatgtgtt gttgcagggg aacggtctgc
       61 accatcccct atatcgatca atataacaaa aatgacaacg tcagattcag ggcacaacct
      121 aaatatattt taggtcacct cccaaatcgt ttgcctgata ccgctccatt ttttaataaa
      181 aaatcaattg tttttgaatc tttgcttttc attgcattgt caagcatagt gtccccacta
      241 tttgcttttt gaaatctttt gtttacatca accaatccat tactaataaa atattctagc
      301 actttatagt tggcactatc ttcactaagt aaatactcaa catcatacag ctcacaatac
      361 atattgttgt tcattttctc catctctgcg acatcttgtt ttgtaaaatt aaatttagat
      421 agtaaataaa gtgccatatc agattttgca ttgcctagag aataagcaag agcttgatga
      481 gccatttttt tatcagtcac cttattgtta atttcttcat aattggatgc tttaatggca
      541 tcctccaaag atatatctct gagtcttatc tttttgaagt tatctgtgct tagcattctt
      601 ggtatatgat actcaagctg aaacacaaag tttataattc tatccagact aacaggatca
      661 atgtttttat tataacaaaa ctctcggaat ctttgatctt tactctttct tataaaatca
      721 ataagatgca acccaattcc aagcctcata tctttagtat taaatattgg cacatcagtt
      781 tttccctgat taccatgcac atacctgcta acttcatcca caacctcttt aaagttatct
      841 aagaaaaatg attggtgctc atggtaaaaa acaggtggaa tagcattatc aaagtggtcg
      901 gttaatgtca tatatccata tggagagtca tgatcaatga tatacgcatt agcaccaaaa
      961 tccacagtat gatgtgtttt atttagtggg agtgtttctt gatgattgga aatttcaacc
     1021 ccaaaaaaaa caaaatcgtg gttagctatt tcttcaatat cctcagagaa tgtgttttta
     1081 tatgtttgta tattctcaga cttaatttta tcaaaggact taatattaag agtattattt
     1141 gacaggttag cgttactttg atgtctgaag ttaaaatcag aattaataat aatagagagc
     1201 aagtgacgtt catccgaaga aagaggatga gttttttgta ttatttcaga aagatagtgt
     1261 ctggacacct tatcgaggtg ctcaagtgac cgaaatgatg accgtgctcc agatggcgct
     1321 tcaatagaaa gatttaatga tttgaaactg gtcccttgag agaacatttt actataactt
     1381 tgcgctgcta tagacttcct taaaaagctt ttcacagtat cagcacagtg atctaaatgt
     1441 ttttcactta tgccagaaga ggatgagttt gttacgcatg atatgttttt ttgtaatgct
     1501 tttgtatctt tgttcggatt tatttttcca tcaacaagat caatattgtt ctgaacatta
     1561 atatgattta ctgcttcagg tattttcata aaacatcccc cattatgtgc gtttttatct
     1621 gcagttattt agaataatat tatctaactt aatattttct tgtatgtatt ataacgaaca
     1681 tattttaata tgttgtacga ttttcactct gaaatcccct gagtttaatc acacttcctt
     1741 tactgaaagg aattattgca acagtattat ttgtttttgg atatatatct tgcgccttgt
     1801 tatgtagtta atgctcagaa tatgatagat aaaatagaga cgcaaaacag ggcatgctca
     1861 taacatgatg ataacataaa aaattagtaa caataaatga aagaatcatg ggcgctcaac
     1921 aaaaagaggg taatgctgcc aacttactga tttagtgtat gatggtgatt ttaaggtgct
     1981 tgcgtagctt ccatttccat cagatgtcct tcctgctccg ctactgaagg cgtggtgcgt
     2041 aacggcaaaa gcactgccgg acatcagcgc
//
LOCUS       DAEEGA010000246         2038 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEEGA010000246 DAEEGA010000000
VERSION     DAEEGA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.251"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..388)
                     /gene="istB"
                     /locus_tag="KLH46_004296"
     CDS             complement(<1..388)
                     /gene="istB"
                     /locus_tag="KLH46_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HBE0380700.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVR"
     gene            complement(385..741)
                     /locus_tag="KLH46_004297"
                     /pseudo
     CDS             complement(385..741)
                     /locus_tag="KLH46_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            821..2014
                     /locus_tag="KLH46_004298"
     CDS             821..2014
                     /locus_tag="KLH46_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HBE0380701.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
BASE COUNT          532 a          466 c          499 g          541 t
ORIGIN      
        1 cacggactgc ttcatagccc atcgctattg ccagatgggt tttccccaca cctgatggcc
       61 ccagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac gactggagtt
      121 gcttctgcgg tgctccggtg gcgaatgtga agtcatactc ttcgaacgtt ttcaccgccg
      181 ggaaggctgc cattcgggta tacatcgcct gtttacgttg atgacgtgcc agtttttctt
      241 catgaagcag atgctccagg aagtccatat aactccattc ctggtctact gcctgttgtg
      301 acagcgcagg cgctgcgctt ataaggcttt ccagttgcaa ctgtccggcg agcgccatca
      361 gtcgttgatg ttgcagttcc atcatcacgc cactcctctg cagaatgagt cgtagatgga
      421 gagtggatga tgctgggggg ggttgtcgaa gctcaccaga ttttcaccag gatgcacgtc
      481 atactctttt ttctccggag gcagtgccag catggactgc tgctcttcga gccagcgatc
      541 acagggacgg gcctggattg tttcatgctt tcgttggtta gcgacatcgt gcagccagcg
      601 cagaccgtgg cggttggctg tttcaacatc gacagtgctc cccatcgggc gcaggcgagt
      661 cattagtggg atgtaaaaac tgttacgggt gtactgcacc atccgttcca ccttaccttt
      721 agtctgtgcc ctgaagggct gaggaatccc cagaaaacga gacaaacata gcattatgtg
      781 atctactgat ttgtgccaac aattcaatga gatcactgct atgcctgttc ataaagtatg
      841 tcagaatttc ttccgggatg ccttagcacc atttcatcag taccgacaaa atgccctgat
      901 ggatgccaca atggcgttaa tcaacggagc atctctgacg cagaccagta tcggacgttt
      961 tttgccgggt aatgcacaag ttaagaataa gatcaaacgt attgatcgcc tcatgggtaa
     1021 tgaggcgctc catcgcgata ttccaatgat tttcaggaat atcacttcaa tgctgacacg
     1081 gcagttatct ttatgtgtaa tagccgtaga ctggagtggc tatccctccc aggagcatca
     1141 tgttcttcgt gccagccttc tgtgtgacgg gcgttccatt ccgttattga gtaaggttgt
     1201 tccttctgaa aaacaaaata atccattgat acaacatgat ttccttgatt cccttgctca
     1261 atccctgccc cccgatgcca gggttattat tgtcacggat gctggatttc agagcgcatg
     1321 gtttcaccat attacttctc tgggctggga ttttattggt cggatcagaa ataacgttca
     1381 gtattgcctc gataacgcgc ctgaaagatg gttaaaagtg tcggatagcc ctgaatgtaa
     1441 aacaccggaa tatatggggg ctggaaggct ggttaaagaa aggaaaaaaa gcatccgggg
     1501 gcatttttac acctataaga aatcggcaaa agggcgaaaa aagaaacgtt cgaaaggtca
     1561 gagtggttta aacaaaacag acaaagaaca gagcaaatca gcaaaagaag cctggttgat
     1621 cttcagcagt acaaatgact tcagagcgcg agaaatcatc aagttataca gtcggcgaat
     1681 gcagatagag caaaactttc gtgatgaaaa gaacgggcgc ttcggatttg gtcttcgggc
     1741 cagcaaaagt cgttcaacag gaagaattct ggttctgagt ctgctggcaa cgttgagcac
     1801 tatcgtaatg tggttactcg gttatcacgc tgaaaataaa gggttacacc tgaaatatca
     1861 ggctaacagc attaaatccc ggcgggttat ctcatatctg acgttagcga agaatgttct
     1921 tcgacactcg ccacttattt taagacgaac agtactgagc acagttctaa accatctgtc
     1981 cagaacctac cgaaatatgg tgctggttta ttagcagtaa ttttgtgggg atccctca
//
LOCUS       DAEEGA010000247         2033 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEEGA010000247 DAEEGA010000000
VERSION     DAEEGA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.252"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..>2033
                     /gene="sat"
                     /locus_tag="KLH46_004299"
     CDS             <1..>2033
                     /gene="sat"
                     /locus_tag="KLH46_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HBE0380702.1"
                     /translation="GMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGIA
                     SVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQEK
                     ITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKESIV
                     NWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGKVI
                     LNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVLAI
                     NNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQGHA
                     TEHAIFRSTASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDWKT
                     GVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGFDF
                     KQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIAQ
                     NSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTG
                     NIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNAI
                     WHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQAD"
BASE COUNT          746 a          358 c          401 g          528 t
ORIGIN      
        1 acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata ttcggagaca
       61 gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta gtcggaacaa
      121 cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa tacaatgata
      181 aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat ggcaataatg
      241 taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc actggtactc
      301 aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat gttctgttta
      361 aggataattt ggattttggt agcggtggta ttatctttga cgaaggccat gaatataaca
      421 taaacggtca gggatttaca tttaaaggag caggaattga tatcggaaaa gaaagcattg
      481 taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc cccggtactc
      541 tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat gttattctta
      601 atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag gtaatactaa
      661 ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact aaacgtggtg
      721 gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc actgacgatg
      781 gaacaacaat aactaactca gatacaacga aagaagccgt tctggcaatc aataacgaag
      841 actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac aatattaatt
      901 ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac acaaaaaatg
      961 atgttgaagt cagtaatgcc agtcttacca tgcaaggcca tgcaacagag catgcaatat
     1021 tcagaagcac agcgagtcat tgctccctgg tatttctttg tggaacggac tgggtcaccg
     1081 ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac aaaagtaata
     1141 atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa tttgatacat
     1201 tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa ggaaatatat
     1261 cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat aatcttgcag
     1321 ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact aatctatcca
     1381 taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata gccataggtg
     1441 atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct ataagtatag
     1501 ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt attgatatct
     1561 ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta acgccgggtc
     1621 tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc attgccagaa
     1681 atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt actctgggac
     1741 agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca gagactcttt
     1801 tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct acagttagca
     1861 tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta gaaacaaaag
     1921 acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca gtggacaacc
     1981 ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca gat
//
LOCUS       DAEEGA010000248         2033 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEEGA010000248 DAEEGA010000000
VERSION     DAEEGA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.253"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..363)
                     /locus_tag="KLH46_004300"
     CDS             complement(<1..363)
                     /locus_tag="KLH46_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0380703.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(430..1644)
                     /locus_tag="KLH46_004301"
     CDS             complement(430..1644)
                     /locus_tag="KLH46_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HBE0380704.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          493 c          412 g          494 t
ORIGIN      
        1 cagaccgata cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg
       61 cattaatgac cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag
      121 caaaataccg gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac
      181 accaaactct gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa
      241 tgacgaacgg tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt
      301 ctgaatacga cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc
      361 catttttagc tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc
      421 gaaccgttat taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc
      481 cgggtcatac ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc
      541 atcgcctggg tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat
      601 ttgttcggaa tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg
      661 cagcgccgcc gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac
      721 ctgataaagc gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt
      781 gatcgtcgtg cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat
      841 actgttaaac acttcaccga ccgcaatcgg cgtcacggta tgctggcgaa tcagacgaaa
      901 acattcctgg ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat
      961 gctcttccca aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag
     1021 atgttcgtta aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag
     1081 atacttttct gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata
     1141 cgccagtcct ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg
     1201 cacacgaatc gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc
     1261 aatcgtacgc ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag
     1321 cagttgatac aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat
     1381 ggcagaaatg gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa
     1441 ttgccagata tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc
     1501 tttcagataa gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca
     1561 aagtccgctt tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa
     1621 aacatctgca gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa
     1681 acctaatttt acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac
     1741 attaaaaaca aaaatttcca ccgaggagaa aaaataccct caaaaaccac ctgaataatt
     1801 aaccattatt gttaaaacaa cacgttaaat aatcacataa caaactccat taatcattga
     1861 aaatgagcac agaaatagaa attaattata ttcaattaat taaaatgtgt attgcttgat
     1921 gtatttaaaa atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa
     1981 ggaatgtgac aaataatggt tctggttggg cgttaaattt aaggttacca gaa
//
LOCUS       DAEEGA010000249         1986 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEEGA010000249 DAEEGA010000000
VERSION     DAEEGA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.254"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            441..536
                     /locus_tag="KLH46_004302"
                     /pseudo
     CDS             441..536
                     /locus_tag="KLH46_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            534..664
                     /locus_tag="KLH46_004303"
                     /pseudo
     CDS             534..664
                     /locus_tag="KLH46_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            773..1039
                     /gene="ospE1"
                     /locus_tag="KLH46_004304"
     CDS             773..1039
                     /gene="ospE1"
                     /locus_tag="KLH46_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HBE0380705.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1430..>1986
                     /locus_tag="KLH46_004305"
     CDS             1430..>1986
                     /locus_tag="KLH46_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBE0380706.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNQ
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          666 a          417 c          337 g          566 t
ORIGIN      
        1 tctcgtctgg ggaatatctc cccggcagcc ttcaggataa aatattatca gatgactgct
       61 tagaaaaaga acaaatggtg gtgtccgcta ttgccagtac acctcaagct tcttaccata
      121 tttgaaataa agctcaatag cgcgaatttt ttgttcttca gtaaacatgt ttcatcttct
      181 gagagtccag gaaatcgtcc gcatccccat taccgtctta tatcactggc gctgacatgt
      241 gctcattgat gaagtgaatc tgaatgacat gttttgccat atcaacgcca accggtgtat
      301 atttcatttg tggagtctcc agtctgagga cgctttatgc atcccgtatt gggcagtatg
      361 atgccggaaa gctgcgaggc tccacacctg ccagtttcac ccttttaacc atcatactac
      421 tccgtgttta attgtgtggg atgcgttcat tacattttaa cagtgtcacc ccgtcggcca
      481 cgaaagataa acaggtgacc agaaaaggga tcttcatcca gcacatgcta tatcttgaat
      541 gtaacaacct gaccctccgc acccgcatca agcggctggc acataaaaca atctgcttct
      601 cgcgttccgt ggaaatccac gaaaagtcat cggctccttc attgaaaaac acatattcta
      661 ctgattggaa gcgccacccg aaaaagtatc gtttttttac agtaaacttc atttagccga
      721 ctataatgta aaaaaataaa ttttttgcca aaatataaca taaggaagta atatgcttac
      781 acaaactata ttcccttgtc ttccacaaaa acaagaaaat atcatccttg aagtatcaaa
      841 tcctgtatta ttatcttcta ctgttactac agatggatat actgttttca ataaaaaagc
      901 agctatatac gaattacaaa tacctgctgc cagccggacg aaaacgttga aattcactgc
      961 tacagaaatg caatggttga caaagataaa tgaagccgga attgatgaaa aacaaagcca
     1021 acggtattct gatttctgaa gttaatttca gactcgttta agctccattt tcaaaaaaat
     1081 atatcagggt ttgttactaa taacttctga aaacagaaaa aaaaatagag cagtaattct
     1141 atttcagaga ggagtaactc catctatcct cgatacgtac tgaacgttat cgtgatatat
     1201 tcatcatgct ttcttcacca ttattcgagt atagggagag acgcaatgac cttaaatacc
     1261 tttttatggc tgaattacca ttctcaggaa taacagcaga ctccttccca tagactgctt
     1321 agggggaaag caatacccgg agaaagggta caaagcaaac tgttttaaaa ttaataagct
     1381 atattctatt taactagaat tcttatcgtt gataagaatt ctagttataa tgattaaatc
     1441 aaccaatata caggcaatcg gttctggcat tatgcatcaa ataaacaatg tatactcgtt
     1501 aactccatta tctttaccta tggaactgac tccatcttgt aatgaatttt atttaaaaac
     1561 ctggagcgaa tgggaaaaga acggtacccc aggcgagcaa cgcaatatcg ccttcaatag
     1621 gctgaaaata tgtttacaaa atcaagaggc agaattaaat ttatctgagc tagatttaaa
     1681 aacattacca gatttaccgc ctcagataac aacactggaa ataagaaaaa accaattaac
     1741 acatctccct gatttaccac caatgcttaa ggtaatacat gctcaattta atcaactgga
     1801 aagcttacct gccttacccg agacgttaga agagcttaat gcgggtgata acaagataaa
     1861 agaattacca tttcttcctg aaaatctaac tcatttacgg gttcataata accgattgca
     1921 tattctgcca ctattgccac cggaactaaa attactggta gtttctggaa acagattaga
     1981 cagcat
//
LOCUS       DAEEGA010000250         1974 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEEGA010000250 DAEEGA010000000
VERSION     DAEEGA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.255"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(1..836)
                     /locus_tag="KLH46_004306"
                     /pseudo
     CDS             complement(1..836)
                     /locus_tag="KLH46_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            1313..1549
                     /locus_tag="KLH46_004307"
     CDS             1313..1549
                     /locus_tag="KLH46_004307"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380707.1"
                     /translation="MSALKETASMLKLFNEAKTQAEVDRAVSEINSKLSSIQLEYLSL
                     IDELTISKSNEAALKAKLTEIEKFKFQSEDCEPR"
BASE COUNT          533 a          464 c          456 g          521 t
ORIGIN      
        1 gctgctctac ggtcatattt ttaattgcgc tccggtttac aagagcccac ccctgttttt
       61 ccagataaaa ctggaaagta tccagggtac agaccagtgc accgtcagga acagtttcgg
      121 tgaatctgat attgccgtgt tcgtcgaagt gcacaaccag agtgcgaccg ttacccggaa
      181 tcatcttgtc agcggacggg gtattattct ggtgcagttc tgcctccatg cggtcgaaca
      241 tcagcgatgt aggcctcttt gaatgcagcg gcttttttcc cagtgaagcc catcaccagg
      301 aaaacgaagc cgtttttggt gatttggtac attggacgtt tttcgccttt ggtgtcggtg
      361 taggtgacgt cctcaaaatt gagggcgtta aattcaggtg agcattcagt atttgcgatg
      421 gcacgtaaca cattgtcgtg tcgtttgtgg aagaactctg caaccgcaac agacgtagtg
      481 acagcgcgac cgttttcgat ggttacgtca gggtgagaaa gggcagggat agtagccatg
      541 atggcatcct cgagtgataa gttaattaac tcaccaccga ggttttccac gaccataagg
      601 gtggtgagac gtacaggggt ggaaataccg gtcactcgag aacccggcca gtcttgcgac
      661 tgccctgcac atcccaccat aatttgaatg gagctgtgca ttacgcataa aaaaaccgct
      721 tcagcgcggc tatgcgctcg agtaacgttc gggtttccac gcccggcccc cgttttatga
      781 ggtgcagatg cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc
      841 tcttaccttt catcaccgaa gtgaactttg ttgatgcggt gcctggtgcc tccaggtgac
      901 gttaaccagt taacaattaa cgccggatac agggaaaccc ataaccaccc gatacgtggg
      961 aactaatcgc tttttaactg ttccgcgtgc gctcagccgc attcaccgca tcacaaaatt
     1021 cactttaaaa agggcggaca tcagccagca atgaaactga tgccgccaaa ggtaccaatc
     1081 aacatggagt gttgtggcgt ggttgtcact taagcgtatg gtcaacctga caacccggtg
     1141 tcctcaacgg ggaaggaata actccgccat acttaccgcc gcgtcatttc gcgttgtgtg
     1201 cctgctttta actacgtcag gcgaggtggt tcctgttatt ccccaacaac aagaaatttg
     1261 tataatctga ataccccaac aataagaaga gtgagcaagt gatgtattag cgatgtcagc
     1321 tctcaaagag actgcatcaa tgttaaaatt attcaatgaa gcaaaaactc aggctgaggt
     1381 tgatcgcgct gtgtctgaaa taaactctaa gttgtcaagc attcagcttg aatatttgtc
     1441 tctcattgat gagctgacaa tctctaaaag caatgaggct gcgctcaaag caaaactcac
     1501 ggagatagaa aagtttaagt tccaaagtga agactgcgaa cctcgctaat tgattactgg
     1561 aacgattgtg tatgccagac agtgcaatac tagctctggc aagattacca tgtatttttg
     1621 tccagaatgt tttgcgaaga gagcaatatc tatattgcaa cctttcccta tcgaggaagg
     1681 cgataaatat acgaaaactc aatgcccctc ctgtaaaaat gagtatctca tggactacaa
     1741 tttcaataat gatttttgag tcaaggtatt cagattttta aagaacatgc cggatactca
     1801 cccgtgtccg gcgcacgcac tccacctcac ccgtggagaa ctccttaatt accaaccttg
     1861 gcttcgttgg ttagccatta acgctggtat gtaatcattc tggcaatgct taatttgaag
     1921 tggcacactg aatttggcca cctgaacaga ggtgatatgc tcacctcaga acaa
//
LOCUS       DAEEGA010000251         1974 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEEGA010000251 DAEEGA010000000
VERSION     DAEEGA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.256"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(38..583)
                     /locus_tag="KLH46_004308"
                     /pseudo
     CDS             complement(38..583)
                     /locus_tag="KLH46_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1238..>1974)
                     /locus_tag="KLH46_004309"
     CDS             complement(1238..>1974)
                     /locus_tag="KLH46_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380708.1"
                     /translation="NGAFNPAKDAPSTTVIKGASVTVTPVLNPETNKTGILCSAATHV
                     SDLDMMGGWTEGALIQTVCDMQVQYCRQMFAAVVDVLKDTSDLQIIEAAPLSGKPSDQ
                     AEDLLDTLALNLPVELGNTLSDYAVLVPEKLEAILDRAAQRAGHEGISELLGCTVCGY
                     AGDDTGVYLLPKRFASISFRSTKDAKTVDVKVTRNSNTAGYDLELISVVDVLATGSVK
                     VKAGEFDVEEDASFPLVHVIRFTAAE"
BASE COUNT          552 a          448 c          429 g          545 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcagaat tccatccagt cgttttgggt
       61 tgctaatgat gtcctgcatc cgcttaatct ggtcgggcgt agacatataa ccccacaggg
      121 tgagcagaac cttttttgcg gctacactat ttccttggtc tgatactaat gccatatagc
      181 cccataaact atccctcagc cagacagcat catagtgggt ttctgccgct gttttgtcat
      241 cttcgaaagt agaagcaaga ataaacgttc cccagggttt ctccgtcaac gttaactttc
      301 gtgtgttttc tgttaatgtt agcaatcctt gagtttcgtc tttggtatag tgactcttta
      361 tttcattttt taatttggct gagtgaatca gaagacgata attaggatac ttatctggat
      421 ttaacgataa ataactttta tctgttgctg cccccctgga attaagaatg ttaaatacag
      481 attcgcttgt cacttctgcc tctgtcgcct ggcatgtgcc gcacgaaaat ataccagcga
      541 gcagaagagt taatgcagat ttccctttca taaaataatc catcaatcgt ttgttaaaag
      601 cgtagataac cacttattca acaacttgaa aataaggtat tttacctggg ttgttacaaa
      661 aggattgcat tgcgtaaacg ctttttattt acaaaaaaat ggggaagtgt tacagcgatt
      721 gcgaaaagcc tgaagtgatt gaattaagat aatatagaaa atcagataca gcaaaaatgg
      781 cagattgatc aggtgaagat aaattggagc gggcgaaggg aacctaggta tcgagggtat
      841 cgaagataat cagccgctaa ccatttgttt ccttaaagga tgatgaatcc ttgtggttac
      901 agttatggtt acacattaat ttatctctgg tcaagtcccg aagtcctaac tttaagacag
      961 tggccccaat cgtccagact tgaaaggggc ctatggggga agtcgctgac ctttatctat
     1021 taaataccta cagagatttt tgtgagattt tttaatccaa ggtcataacc tactgccccc
     1081 acaggccgca aagtgaagac ctgtaagcac cagagcaata gcgagtatct atcactccct
     1141 gtagagtatc tggtagacca agaggacaca gaggacgacc atatacaact cctgtaagct
     1201 catggatgta ctccttatgt tcactcaggg tttatagtta ctctgcggca gtaaaacgaa
     1261 taacatggac caacgggaaa gaggcgtctt cctcaacgtc aaactcacct gctttcacct
     1321 taacggaacc agtagccaac acatcgacca cactgattaa ctcaaggtca tacccagcag
     1381 tgttgctatt tcgggtcact ttgacatcta ccgtcttagc gtctttagtt gacctgaaag
     1441 agatagaagc gaaccgctta ggcaggagat agacacctgt atcatcgccc gcatagccgc
     1501 atacagtgca gcccaatagc tcactaatac cttcatgacc tgcgcgttga gcggctcggt
     1561 ccaagattgc ctcaagtttc tcagggacca atacagcgta atcgctcagt gtatttccca
     1621 actcgaccgg aagattcaac gctaatgtat ccagaagatc ttcagcttga tcagacggtt
     1681 taccacttag tggggctgcc tcaatgattt gaaggtctga cgtatctttc aggacatcga
     1741 ccacagcggc gaacatctgg cggcagtatt gaacctgcat atcgcataca gtctggatta
     1801 atgccccctc ggtccaaccg cccatcatat cgaggtcaga cacatgagta gcagcagaac
     1861 acaggatgcc cgtcttattg gtctcagggt tcaacactgg ggtgacagtt acgcttgccc
     1921 ctttgatgac tgtggtagac ggagcatcct tagctggatt aaacgcgcca ttat
//
LOCUS       DAEEGA010000252         1936 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEEGA010000252 DAEEGA010000000
VERSION     DAEEGA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.257"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="KLH46_004310"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="KLH46_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HBE0380709.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1936
                     /locus_tag="KLH46_004311"
     CDS             1494..>1936
                     /locus_tag="KLH46_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBE0380710.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          552 a          337 c          466 g          581 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
     1441 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
     1681 gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat catgaacaga
     1741 tggcgatgga tgcgctggca ttccagcaga tgtctttgtg gttgatgaat ggcggcgact
     1801 ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc
     1861 agcatttgcc ctttattctt gaagtacaca gtcgcaccgg caagcatgtt attgctcatg
     1921 ccgattatcc agatga
//
LOCUS       DAEEGA010000253         1921 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEEGA010000253 DAEEGA010000000
VERSION     DAEEGA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.258"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            193..462
                     /locus_tag="KLH46_004312"
                     /pseudo
     CDS             193..462
                     /locus_tag="KLH46_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            459..685
                     /locus_tag="KLH46_004313"
                     /pseudo
     CDS             459..685
                     /locus_tag="KLH46_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            714..1304
                     /gene="ospG"
                     /locus_tag="KLH46_004314"
     CDS             714..1304
                     /gene="ospG"
                     /locus_tag="KLH46_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HBE0380711.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1643..1921
                     /locus_tag="KLH46_004315"
                     /pseudo
     CDS             1643..1921
                     /locus_tag="KLH46_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          539 a          417 c          459 g          506 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgactaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaatctct tgtgattttt aaatatcgta acaaaggtat gacatgaaaa
      721 taacatctac cattattcaa acaccttttc catttgagaa taataattct catgctggca
      781 tagtaacgga gcccattctc ggtaagttaa taggtcaggg gtcgacagca gaaatctttg
      841 aagatgtgaa tgattcatct gctttgtata aaaagtatga tcttattggc aaccagtaca
      901 atgagattct ggaaatggct tggcaagaat ctgagctttt taatgctttt tatggcgatg
      961 aagcatccgt tgttatacag tatggcggag atgtgtacct ccgaatgctg cgcgtgcctg
     1021 ggactcccct tagtgacatt gatacagctg atatccctga taatatagag agcctttatc
     1081 tacagttgat atgtaaattg aatgagttga gtataatcca ttacgatctt aatacaggta
     1141 atatgctgta tgataaagaa agtgagagtt tattcccaat agattttcgc aatatttatg
     1201 ctgaatatta cgctgcaacc aaaaaagata aagagattat cgaccgacga ttacaaatgc
     1261 gtacaaatga tttttattcg ttattaaaca ggaaatattt atagacgtat ttgttgatgc
     1321 tataagccag aggctcttct atgagaatat ggggaccatt ccctcgcgat attaacgtaa
     1381 tatcttaatt gtatgcaccg ccagaatgcg ttcagtgcga tcacggcagc agacaggtaa
     1441 aaatagcaac aaaccacccg aaaaaccgcc gcgatcgcgt atgataaatt ttaaccacat
     1501 gcatacctgt gcagcaaggc gaatcacgca cggatttcca gccagagaca cctgaaacgt
     1561 aaccggctca tttaaaccgt ctggtctgtt acctccggtt ccacaaaaat aatgtccatc
     1621 attttgaagt ggacactatc gtatggaata ccggacctga atcactgaag ctttacgcct
     1681 tcatttcgaa gaacatttac cacgggttgt ggccggatgt cgcctgggcg taccaaaatc
     1741 aactgtctgt ggtatgttcg tgcgcttccg caacgctggc ctttcatggc ctctgcccgc
     1801 aggtatgtcg gagcaggaac ttgatgccct tctttacgga agtgcctcca cagtacctgt
     1861 cgtacttact gaaagcaccg ttatgcccaa actcccggtg gtaaaaaaac gtccccggcg
     1921 g
//
LOCUS       DAEEGA010000254         1913 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEEGA010000254 DAEEGA010000000
VERSION     DAEEGA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.259"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(53..1702)
                     /locus_tag="KLH46_004316"
     CDS             complement(53..1702)
                     /locus_tag="KLH46_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBE0380712.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALTVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          451 a          506 c          492 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accgtcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAEEGA010000255         1875 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEEGA010000255 DAEEGA010000000
VERSION     DAEEGA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.260"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(854..1327)
                     /locus_tag="KLH46_004317"
                     /pseudo
     CDS             complement(854..1327)
                     /locus_tag="KLH46_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1255..1621)
                     /locus_tag="KLH46_004318"
                     /pseudo
     CDS             complement(1255..1621)
                     /locus_tag="KLH46_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1623..1875)
                     /locus_tag="KLH46_004319"
                     /pseudo
     CDS             complement(1623..1875)
                     /locus_tag="KLH46_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          417 a          531 c          446 g          481 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctactcgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacctgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccaaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgt atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggc gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgctgacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttga
     1621 tgacggttta cgcggtcgcc tgcggcaacg gctttccggc tgacggtagt gtggaccttt
     1681 ttaccccgga gaacaccggc aagccccata accgccatga gacgtgccac agtgcatctg
     1741 gccactctga taccttcccg taacaactga tgccagactt tacgcacagc gtatacctga
     1801 tgattctcat cgtatacgcg cagtatctct ttcttcagcc agtcatcgcg ctgcgcacgg
     1861 gcactgcgtt tatca
//
LOCUS       DAEEGA010000256         1850 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEEGA010000256 DAEEGA010000000
VERSION     DAEEGA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.261"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..258)
                     /locus_tag="KLH46_004320"
     CDS             complement(<1..258)
                     /locus_tag="KLH46_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380713.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            283..522
                     /gene="relB"
                     /locus_tag="KLH46_004321"
     CDS             283..522
                     /gene="relB"
                     /locus_tag="KLH46_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HBE0380714.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            522..809
                     /gene="relE"
                     /locus_tag="KLH46_004322"
     CDS             522..809
                     /gene="relE"
                     /locus_tag="KLH46_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HBE0380715.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            881..1036
                     /gene="hokD"
                     /locus_tag="KLH46_004323"
     CDS             881..1036
                     /gene="hokD"
                     /locus_tag="KLH46_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBE0380716.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1254..1469
                     /locus_tag="KLH46_004324"
     CDS             1254..1469
                     /locus_tag="KLH46_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380717.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEI"
     gene            1572..1850
                     /locus_tag="KLH46_004325"
                     /pseudo
     CDS             1572..1850
                     /locus_tag="KLH46_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          409 c          467 g          467 t
ORIGIN      
        1 tgacatgcta aagcaaaaaa tagatgaata agttgagttg tgcatatgta gcctgaccgt
       61 cacaaagtat atggtgtctg taccagtaag atgatggccg gactctttaa aaaagagctg
      121 acctgcacaa tacaggatgg acttagcaat ggctgctcct ggcacaaagc ggacagtgat
      181 caccgttctt acgactactt tctgacttcc ttcgtgactt gccctaagca tgttgtagtg
      241 cgatacttgt aatgacattt gtaattacaa gaggtgtaag acatgggtag cattaacctg
      301 cgtattgacg atgaacttaa agcgcgttct tacgccgcgc ttgaaaaaat gggtgtaact
      361 ccttctgaag cgcttcgtct catgctcgag tatatcgctg acaatgaacg cttgccgttc
      421 aaacagacac tcctgagtga tgaagatgct gaacttgtgg agatagtgaa agaacggctt
      481 cgtaatccta agccagtacg tgtgacgctg gatgaactct gatggcgtat tttctggatt
      541 ttgacgagcg ggcactaaag gaatggcgaa agctgggctc gacggtacgt gaacagttga
      601 aaaagaagct ggttgaagta cttgagtcac cccggattga agcaaacaag ctccgtggta
      661 tgcctgattg ttacaagatt aagctccggt cttcaggcta tcgccttgta taccaggtta
      721 tagacgagaa agttgtcgtt ttcgtgattt ctgttgggaa aagagaacgc tcggaagtat
      781 atagcgaggc ggtcaaacgc attctctgaa ccaaagcatg acatctctgt ttcgcaccga
      841 aggtgacact tctgctttgc gttgacagga gaagcaggct atgaagcagc aaaaggcgat
      901 gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg acggcactgg taacgaggaa
      961 agacctctgc gaggtacgaa tccgaaccgg ccagacggag gtcgctgtct tcacagctta
     1021 cgaacctgag gagtaagaga cccggcgagt gagaaatccc tcgccacctc tgatgtggca
     1081 ggcatcctca acgcacccgc acttaacccg cttcggcggg tttttgtttt tattttcaac
     1141 gcatttgaag ttctggatgg tgccggaata gaatcaaaaa tacttaagta gcgcgcaggg
     1201 ataaaaggga tggaccccga acaggggagt gctacttatc tggaaggatt ctgatgatga
     1261 acatcgaaga actgcgtaaa attttttgtg aagatggcct ttatgctgtg tgcgttgaaa
     1321 atggaaatat tgttagtcat taccgcattg tgtgtttgca aaaaaatggg gctgcgttaa
     1381 ttaattttgt ggatgcccga gtgacggacg gatttatctt gcgcgacggt gagtttgtca
     1441 cttcattaca ggcattgaaa gagatctgaa taaaagctgg cttttctgct ttttcagaag
     1501 aataaactca tctacaatct tgcgcggggc tgaactcccg ctgagtaaca ccgtgccacc
     1561 ggagaaaacc gatggcacgc aacgcaaaat attacaatca tgataattcg accgttcttg
     1621 cccacacgca cgagcggtat tctcacgcat ttaagtcaga ctggtaccag catccctcat
     1681 gcactgaaga acaggccgaa tggcttattc agtgttaccg caggcgcgga tgcgaggtta
     1741 aaaaagccct tagcctcgac taccgtcact agataatctc cgtcaggctc ccttactccg
     1801 aacagccacc gcgtccgtcc cgcacattcc agcaacggat ctggaggtaa
//
LOCUS       DAEEGA010000257         1833 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEEGA010000257 DAEEGA010000000
VERSION     DAEEGA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.262"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..180)
                     /locus_tag="KLH46_004326"
     CDS             complement(<1..180)
                     /locus_tag="KLH46_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBE0380718.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(321..875)
                     /locus_tag="KLH46_004327"
     CDS             complement(321..875)
                     /locus_tag="KLH46_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBE0380719.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(872..1162)
                     /locus_tag="KLH46_004328"
     CDS             complement(872..1162)
                     /locus_tag="KLH46_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBE0380720.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1162..1761)
                     /locus_tag="KLH46_004329"
     CDS             complement(1162..1761)
                     /locus_tag="KLH46_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBE0380721.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          497 c          421 g          470 t
ORIGIN      
        1 gttatgggtg atcggggaga gagtttcaac agagatttga gtggtcataa cgataactcc
       61 gtacatttgg aaattatcgc caccgacgac gccaatcgaa ctggtggcga actgtgcagg
      121 gttggcgtaa ccgggtacgg aaaccggcga gcctttcggc tcccccacac agcccgccat
      181 aaatcgcgaa tgtgactgtg caaacgatat gaaaaaagac gcgggcgcgt ctcatatcgc
      241 tccgtaaaca tccgggacgc caatcccgac gccagatttt gctggcgcgt gaggaatata
      301 gccccggata acagattgag tcaacaaacg gtttttagat ccccggaaga gaatgcatca
      361 cgcatcggta gatagagcat atattcagca agatttagcc atacatctac ccgactgcag
      421 cacgtccgga aacaccactc agggtgtgcg tcattcagca attcagccat tttacgctta
      481 gtcatccccc gcccttcata ccgttgccgg aggacactaa tcaatccagg atgctctgcc
      541 agcacctcac ttatgactct atcaatacat aacgcctctg catcagtaca atgcgccagc
      601 caggtctttt gcttgccatt gatcatctct cgcaaaaacg cttccagctc aggtttctct
      661 attcccgctt ttttcatcct gcgcagggct tcattgacgg ctgttttcgt caattttttg
      721 gatgccagca actggttaaa catatttccc gtcttaccgc cgccaatata cgaccagcgc
      781 ccccacatgc gcagttttcc ctgaatccag acactttcca gcgtggtgag acgaaggtgt
      841 tccccgcttt tgcctgtatt tgttgggtaa atcataaata accttccttt ctccagattt
      901 cttgtgtgcg aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc
      961 tggtttttac ccgcccgtcg attaaatcgt ggcatgagct acaggcaatc gctgcctgca
     1021 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
     1081 aagtttcggg attgtgattg cagtagccag gaattctgac ggtgcacatc tgcccccgcg
     1141 ccgctttacg taaatccacc attacgcaaa ctccagtaac tgcgcggcca cattttcgac
     1201 ttcctccgga gaggaaaatt tacggaacag gatccagttc cacagcacat tcagtacaga
     1261 tttataaacc tgctgaaact cggtttcgtc catgttcgca aatgcgatag attttgccct
     1321 gcgcccacga ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac
     1381 ccactcgcgg aaagcgtcaa acgactttag caatgccgta tcccgggttc tgcatgtcgc
     1441 aactgtatta aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac
     1501 tgattcgcac aggtaatcaa cgaagcctga taccagtttt cgttcgcgag gcgtgatcgc
     1561 cccaccgacc ggagtccagt aatcaaatcc cagttgcagg agtttgaaaa aacgcttgtg
     1621 gaatgcgtag ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg
     1681 atgcaaaaaa tcgcaactct ccggcgtcgc cgggagaagt aatccggaag aggtttgttt
     1741 gaccagttgt atatgcgcca tcgtagttct ccgctggcgc agtagaatgg gtgttcagcc
     1801 cgttatgtag tataccagaa ttaatgccaa tac
//
LOCUS       DAEEGA010000258         1826 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEEGA010000258 DAEEGA010000000
VERSION     DAEEGA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.263"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(1..335)
                     /locus_tag="KLH46_004330"
                     /pseudo
     CDS             complement(1..335)
                     /locus_tag="KLH46_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(335..1255)
                     /locus_tag="KLH46_004331"
     CDS             complement(335..1255)
                     /locus_tag="KLH46_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE0380722.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1393..>1826
                     /gene="pgrR"
                     /locus_tag="KLH46_004332"
     CDS             1393..>1826
                     /gene="pgrR"
                     /locus_tag="KLH46_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBE0380723.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          456 a          440 c          424 g          506 t
ORIGIN      
        1 agcacgccaa tgcgttcagc atcaacatat ggcagagtta ctgcataatc gataacacgg
       61 ctaatatcct ctacgcgctg tgtcggatct tctatccaac gaggctaccc accagattct
      121 ccctggaaac tggcgtcata agcgagaacg acatagcctt tttcagccaa tgccttgcca
      181 tagatattgc cagacgtctg ctccttacaa ctgccaaaag gatggacgct gataatcgcc
      241 ggatactgtt ttgactcatc aaaatttggt ggaaactgaa tattggcagc aatatgccag
      301 gccatatcac tattgcggat ttgaatatgt ttcattacag agtttcctca aagaacggag
      361 ctaatactga aattgcttca gccacatagg ctttgccgtc ataaagatcc atatggtttg
      421 cgccttcaac aatgtgatag cgtttatcct ggcttgatgc ccgatcgtac aggtcgtcac
      481 tcatccattt gctccctgcc tggctgcccg ccacaatctg catcggctga gtcaggtaca
      541 cttccgccat atggtaagca tcataggtaa taatctggtt aaggctgcgc aaagtagcgt
      601 aacccggtgc tgttggatac tgcgcacgag gtgtgtggta atactcccag gcctgacgca
      661 gttcttcgtt cggcgcatcg gcctccttca ttggagccag tggcataatg gcgtattctt
      721 cgctgctaat atcgctggtt cgggcgtttg agccagcttc aatgtgtggc agagcatcaa
      781 tagatttcac attgttttcc caaccattgc ggaacatcga accaatattg accgcactaa
      841 cggtaccgat ggccttgatg cggcgatcct gaattgcagc attggctgta tatcctgcac
      901 cggcacaaat tcccatcgca ccaattcggg tattgtcgac ataagaaagc gttgtcaggt
      961 aatcaatcac ggcactgacg tcttcagtac gaatgtatgg gttttctaac tgacgcggct
     1021 cgccgccact ttcaccttga taagatgcgt cataagcaat agtgacaaat cccttttccg
     1081 ccagcttttc ggcataggtt ccggccgttt gctctttaac gcccccacct gggtgagata
     1141 ccacaattgc cggatactga cgggtttcat caaattttgg agggaaatag atcactgcag
     1201 acaaagagat ggttggatta ttgctgttag tgaagctgac tttattattc atcattcagt
     1261 tcctcatgga gttgtcggtt cgttttgacg gttggtgata tcactataga tattgatcat
     1321 taagttgatt agaccctaaa tcatgattag acctatcatt taaatgatta atagattgta
     1381 tggagatact gaatgaagcg tgaagaaatc gctgatctga tggcgtttgt cgtcgttgca
     1441 gaggagcgta gcttcactcg cgcagcagcc cgcctgagca tggtgcagtc agctttaagc
     1501 cagatagtgc gccgtataga agaacgattg ggattgcggc ttctgacgcg aaccacgcgc
     1561 agcgttgtac ccactgaagc gggcgagcat cttttgtctg ttcttggccc tatattgcat
     1621 gacatagatt ctgccctgac atccctgagc gatctgcaga accgcccatc cgggacaata
     1681 cgtattacta ctgtagaaca tgcagcaaaa acgatattgt taccagccat gcgcacattc
     1741 ctgaaatcgc atcctgaaat tgatattcag cttaccattg attatggttt gaccgatgtc
     1801 gtttctgaac gttttgatgc aggcgt
//
LOCUS       DAEEGA010000259         1809 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEEGA010000259 DAEEGA010000000
VERSION     DAEEGA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.264"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            173..1198
                     /locus_tag="KLH46_004333"
     CDS             173..1198
                     /locus_tag="KLH46_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0380724.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="KLH46_004334"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="KLH46_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          420 c          462 g          506 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctagct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtggg
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgtc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAEEGA010000260         1760 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEEGA010000260 DAEEGA010000000
VERSION     DAEEGA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.265"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(1..263)
                     /locus_tag="KLH46_004335"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="KLH46_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="KLH46_004336"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="KLH46_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HBE0380725.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1175..1645)
                     /locus_tag="KLH46_004337"
                     /pseudo
     CDS             complement(1175..1645)
                     /locus_tag="KLH46_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          501 a          435 c          318 g          506 t
ORIGIN      
        1 agcaagcact gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatctt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg actaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacgc ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa tgacagcgcg
     1621 gacaatgaac agtaacggta gccatggaaa accttaaata ccagtagatt accagaaact
     1681 tctgtcgatt gaagacatca cccgaagttt gaattcattc gaatactttt tacgaggttc
     1741 ttttcgccag tcctgtaatt
//
LOCUS       DAEEGA010000261         1745 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEEGA010000261 DAEEGA010000000
VERSION     DAEEGA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.266"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            452..1300
                     /locus_tag="KLH46_004338"
     CDS             452..1300
                     /locus_tag="KLH46_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBE0380726.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1317..1598
                     /locus_tag="KLH46_004339"
     CDS             1317..1598
                     /locus_tag="KLH46_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380727.1"
                     /translation="MNPMLDYCFTSAPQQTITHTGIKNLLWIIKKNLQQKGDPDLIPC
                     AYGLCDRQVSPDKAYTAELIYMHGTMMRKKKRKYCSEICAEKDQMAHEL"
BASE COUNT          497 a          399 c          410 g          439 t
ORIGIN      
        1 cattatatag ttctactctg gcgagatacg ttggcatatt catttcctta ctggttgtgt
       61 gagaacttca gtaaagatac caccaaagcc cggaagtggt gaaataaaac cgggcacaac
      121 acgaaggcgc atttccggta tccataaaga gtcggtcttg tctgtgaaat ttaaatggtg
      181 ggagtgcgct tccggttgta aataacgaca ttgctgtgtg aagtaccagt tggcggcatc
      241 ggtttaattg ctggctgatg tccgcccttt ttaaagtgaa ttttgtgatg cggtgaatgc
      301 ggctatgcgc acgcggcaca gttaaaagtc atgttagtcc ttattggttt gggtgggaaa
      361 gccaactgta attgttaact ggttaacgtc acctggaggc accaggcacc gcatcaacaa
      421 agttcacttc ggtgatgaaa ggaaagagaa aatgttgaat gtagctattg aaaaccagaa
      481 cgggtggaat tatagtgcac ctgcacctca taaaacgggt gccgggattg ctaccccgac
      541 gatgactact gcgcataacc gcgctcaggc ggttttttta tgcgtcaagc acagccacat
      601 tcagattatg gtggggcgtg cagggcagcc gcaaggctgg ccggtttcgg tagtcaccgg
      661 ttgtagcaac cctgtgcgtc tcaccaccca tgagattgct acctctggtg gtgagtcatt
      721 taaactgact atcgaggctg ccatcatggc tactatcctt accctttctc accctgacgc
      781 aaccatcgaa aatggtcgcg ctgtcactac gtctgttgcg gttgcggagt tcttccggaa
      841 aatgcacaaa aacgttattc agaagatcga aactctcgaa tgctcgccgg agtttaaccg
      901 gcttaatttt aagcccgtca cctataccga cgccaaaggc gaaaaacgcc caatgtacca
      961 aatcaccaaa aacggcttcg ttttcctggt gatgggcttc actggcaaaa aagccgccgc
     1021 attcaaggaa gcctacattg ctgagttcga ccgcatggag gccgaactgc gccagaataa
     1081 caccccacct gctgacaaga tgattccggg tgatggtcgc actctggttg ttcacttcga
     1141 caaattcggc aatgtcgaat tcaccgaaac cgttcctgat ggcgcactgg tctgtaccct
     1201 ggagactttc cggttttatc tggaaaaaca gggatggact cttgttaacc ggggcgcaat
     1261 taaaaatatg actgtggaac aattactttc tttaaaataa aaatggagct acggatatga
     1321 accctatgct tgattattgt tttacctctg cacctcagca gaccataaca cacaccggaa
     1381 tcaaaaattt gttatggata atcaaaaaaa acctccagca gaaaggtgat cccgatttaa
     1441 ttccctgtgc ttatggcctg tgtgacagac aagtaagccc tgataaggct tacactgctg
     1501 aacttattta catgcacgga accatgatga ggaaaaagaa acgcaaatat tgtagtgaaa
     1561 tctgcgccga aaaagaccag atggcacacg aactttaatt aactgactat tcgaaactga
     1621 atttatgcca gcaatggcag ggattcgctc aaccttaatt aaggagaaaa acatgaacac
     1681 caattatgaa gccactgttg caactactga taacattgtt cacgaggttt acctggaagg
     1741 aaagc
//
LOCUS       DAEEGA010000262         1718 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEEGA010000262 DAEEGA010000000
VERSION     DAEEGA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.267"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            390..626
                     /locus_tag="KLH46_004340"
     CDS             390..626
                     /locus_tag="KLH46_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380728.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARA
                     QIESQRYADRQRALREDERQQSDFAWSRPRAPRR"
     gene            840..1244
                     /locus_tag="KLH46_004341"
     CDS             840..1244
                     /locus_tag="KLH46_004341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380729.1"
                     /translation="MKILAAMEIADCDFEHMDMDVLVGALLKLKLWDSHHHNLNMQGK
                     RSFLDDLRADGIEAIKKYEAEIESENESFAEERLILRIYQNELGLWLGRLFCGEAEIG
                     QCGVYGSADEVRTVARQSGILCDSIEIFQKDA"
BASE COUNT          392 a          401 c          485 g          440 t
ORIGIN      
        1 aaaaaagttg tagtgcccgc cgcagtctca cgtccggagc gtagcgaccg agtgagcgag
       61 gaggcatcat ttcactattc gggcatggta ttgcggacgc ttctgagcca catatcacac
      121 ctgccacatg acgcactttc ctgataatcg cacctatgcc aacatattaa gccagtatac
      181 actccgctag cgctacgtga ctggttcagg gctgcgcccc gaaacccgct aaatccactg
      241 acgcgcctgc ggcttgtcca acgcctcacg gcaccggatt aaaatccgct cccgttcacc
      301 gtttctgact gcctttcacc gtcgcttacg cttcatcgtt cctactgtgt ctggcagact
      361 gcaggggcta ttctcatacg gagataaaca tgacgagact gacaaacctg acccccgctg
      421 agaaaaagtt tatcgacgac gctatagccg ctgccgaacg tgcggccggg aagaagctca
      481 atcagcctaa ccggcacatc gtcctgaaca gagcgcgtgc gcagattgag tcgcagcgtt
      541 acgccgaccg ccagcgtgca ttacgtgagg atgagcggca gcagtccgat tttgcctggt
      601 cacgtccgcg cgcgccgcgc cgctgattgc ggattttggc ggtttagtgg tgctgatgct
      661 gcgaaatcac ctgcggttca cccgcgtgca tagctatgca gtcgcgcaga aatcatcagg
      721 ggcgtttttc ctggatgtgg gtgaggaaat gtgccggaaa cggctctgat gcgtcagaat
      781 tgcgctgagg gcctgtcagg gggtgctgat aacggatgtt atggtaaatc ggagggtgta
      841 tgaagatttt ggctgcaatg gaaatagctg attgtgattt tgaacatatg gatatggatg
      901 tcttagtagg ggctttactg aaattaaaat tgtgggatag ccaccatcac aacttgaaca
      961 tgcaaggtaa gaggagcttt ctcgatgatt taagagcgga tggtattgag gctattaaga
     1021 aatacgaggc tgaaattgaa tctgagaatg agtcctttgc ggaggagcga ttaatactga
     1081 ggatctatca gaacgagctt ggcttatggt tagggaggct cttttgtggt gaggctgaga
     1141 tcggtcagtg tggtgtttat ggatcggctg atgaggttcg aacagttgcg cgtcaaagcg
     1201 gtattttgtg cgatagcatc gaaatttttc aaaaggatgc ctaatgcccc cgtggtgggt
     1261 acaccagaaa acggggagcg gagcgaccag cgtcaggtcg ttctatgttg ttctgattgt
     1321 gtgggtgttg tgccagactg ggcaatcgca tttcaccgca actcctgcct tgaatctcac
     1381 tttatcaggt gcctgttatc gtacagcgag acggggtaga gcttaactct ttcgcggtta
     1441 agttacgttt ccaccgtttg cctgtttatc ctgccggagt ccggggcgta gacctgagtt
     1501 tcaagggtgc agatatgatt ttaatgtcgt tgaatggtat taagctggta gtgacaaggc
     1561 tagccagccc tgctaggata ggattgctac gtctgatcct ggcaggtatc tgcgaagaat
     1621 tagagtaccg aagccctgtg attctgtcac ggggcttttg tacatctgta gcctgatctt
     1681 tcaacaagat ccccgtcaca cctcacttca tgtatgtg
//
LOCUS       DAEEGA010000263         1709 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEEGA010000263 DAEEGA010000000
VERSION     DAEEGA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.268"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..89)
                     /locus_tag="KLH46_004342"
     CDS             complement(<1..89)
                     /locus_tag="KLH46_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBE0380730.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLD"
     gene            complement(770..844)
                     /locus_tag="KLH46_004343"
     tRNA            complement(770..844)
                     /locus_tag="KLH46_004343"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(809..811),aa:Gly,seq:tcc)
     gene            complement(848..922)
                     /locus_tag="KLH46_004344"
     tRNA            complement(848..922)
                     /locus_tag="KLH46_004344"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(887..889),aa:Thr,seq:tgt)
     gene            complement(925..1001)
                     /locus_tag="KLH46_004345"
     tRNA            complement(925..1001)
                     /locus_tag="KLH46_004345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(965..967),aa:Arg,seq:tct)
     gene            complement(1003..1079)
                     /locus_tag="KLH46_004346"
     tRNA            complement(1003..1079)
                     /locus_tag="KLH46_004346"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1043..1045),aa:Ile,seq:cat)
     gene            complement(1269..>1709)
                     /locus_tag="KLH46_004347"
     CDS             complement(1269..>1709)
                     /locus_tag="KLH46_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBE0380731.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          468 a          452 c          349 g          440 t
ORIGIN      
        1 tgatccaacc actgcaaaaa ccagtagccg gcactgccgg cggaggtgcc gtaggcaatg
       61 cctgttgata ttttttccat ctgatacata tcccgccccc ccgacagacc tgtgctatcg
      121 gaaagaaaaa aggccatcag caaaactctg atggcctgaa tcacctttac caatattgta
      181 tgaaaaaaca cgcacgacta attgacaata attttcattt ccattaaaaa agatcgcgta
      241 gcattcttaa ttcatgagga actttacccg ccagcaatct gagtagcgaa agctgttcat
      301 ccccaatgtt tttgctggcg ggtccttttt tctacggtcc tcctccccgg agggggcatt
      361 tcattatttt tctaatcgtt actggtgaga aggcggaaga aaaagccagt tcttcgcccc
      421 taaataacag catgattcca gtccatgcaa aatctgattt ccagctcaca cataccgagc
      481 actgtacaaa aaaccgccaa ccaaggcagt taagagtgtg ttcccggggt ttgcttagaa
      541 tattttgtaa gttgtccgaa gagatattta caacaccaga atgatgcatt atcagtcccc
      601 tgccagaggt tcactacgta acctgaccga caatgcacca ttctggtgct gaaaaaagag
      661 cactggaact gcaacaaaac aacatcacag aacaggagat ggtaaggagt cagacattcc
      721 cacacaatat tgtgtcagtg caaataacaa ccttcgtctc aaatctcgct ggagcgggca
      781 gcgggaatcg aacccgcatc atcagcttgg aaggctgagg taatagccat tatacgatgc
      841 ccgcatatgg tgcgactacc ggaatcgaac tggtgaccta ctgattacaa gtcagttgcc
      901 ctgcctgctg agctaagtcg gcgctggccc accaccgagg actcgaacct cgcaccgtca
      961 acttagaagg ttgatgctct atccggatga gctagtggtg gttggtggcc cttgctggac
     1021 ttgaaccagc gacctggcga ttatgagtcg ctcgctctga ccaactgagc taaagggccg
     1081 gaggcagaat aataaccata tgtcatcaca tctgcaaact catctgatca ccagcacgtt
     1141 taacgtcctg tgccgttttt caggtataaa aaaaaccgca taaagcgggc tctttcaaat
     1201 gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa gcgtacaaga attgaagcag
     1261 tttttacgtc aaaaagcaat aacttttttc tctataccaa aagccataac cattggtttg
     1321 tacaaaataa attctgccac ctttagccaa tgctcaatgc gtctttcaca ggttcttaaa
     1381 ctccatttcg ggtgtgcatc attcagcagt tcagccattt tgcatttggt catcccccgc
     1441 ccctcatacc gttgccgaag gacgctaatc aatcctggat gctctgccag cacctcactt
     1501 atgacccgat caatacataa cgcctctgca tcagtacaat gcgccagcca gctcttttgc
     1561 ttaccgttaa tcatatcccg caaaaaagcc tcaagttcag acttgttcag acctgctttt
     1621 ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca gctttttaga ggtcaacatc
     1681 aggttaaaca tattccccgt cttaccgcc
//
LOCUS       DAEEGA010000264         1652 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEEGA010000264 DAEEGA010000000
VERSION     DAEEGA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.269"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..236)
                     /locus_tag="KLH46_004348"
     CDS             complement(<1..236)
                     /locus_tag="KLH46_004348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380732.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEG"
     gene            444..1241
                     /locus_tag="KLH46_004349"
                     /pseudo
     CDS             444..1241
                     /locus_tag="KLH46_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT          493 a          322 c          377 g          460 t
ORIGIN      
        1 ccttcgtcat caacacgtac aaaccagaag accagctttt tgtttcccgc atccacaaag
       61 aacggaatat tcaggtctgc gcagcattca acggcatcgt caaaactatc aaagcgcaga
      121 acttctgcgt cttcttcgtc aaaaaaatca tcttcgtgaa gcttcacgac atagcgggga
      181 agtttgcttc tttgagaggc gggtttacgt ttacggggtt tagctgaacg ggccatataa
      241 ccacctgaaa gacaatgaca ttgcctgttt ttataacggt aattgcagac catgacaagc
      301 cgcagccgtc aggctgccta ctcgataatt taagttcagc agcagttaaa acagcaggtg
      361 tacttatcag ctttcaggct gtactatccg cttatcagcg agtaatggaa gttggccttc
      421 aacgtgcaag tgctgaacgt tcaattgatt tcgtattttc taaagatgca aatcaagtaa
      481 aagatttcac aaagtcatta gctcaaacaa cgggtttaga tatcgctgaa ctgcaatcac
      541 agtttgctgg tttcggtgct tcagctagag agtctctagg cattcaaggt tctgaagaac
      601 tttttagaaa tatgattggc tattctcgtc tgatggggcg aagtgaagaa gagattaagc
      661 gagcactgac agcattatca cagatggctg gtaagggtca gattatggct gaagagctta
      721 aggggcaact agctgaagct gttcctggta tggttcaggt attcgctaaa gcaaccggaa
      781 agactgaaca ggaattattt gatgctatga agaagggggc tttaaaatct gccgatactc
      841 ttcagaaggt cactcaggaa ctgaataaac aaatcaccgc taaaggtggc tggaaagcta
      901 tttcagaaag tacacaggca caactaggga acttgaaaaa ctcttggaat accacgcttg
      961 atagtatctt cagaggttct gaaaatggtc tgcaagattt tacaagaagc ttaaccaatc
     1021 ttttaaacgc tcttggtgga agtggtaaga gtcttggtga atctcttggt agtctcatga
     1081 cttctatgtc acatggtgtc gattcattga ccgatattag ttacagggta agggcgttct
     1141 ttgatgaggt gacattagct taccgtccac taaatgacac tcagaggaaa attgttgatg
     1201 gtttatctgg tgggttaatt gatggtctga agatgcttgc tgtggctgtt actgctcaaa
     1261 aggctgcaaa tgtagcagga ggcttgttaa accttgcaaa cgctgttact tcattcggtg
     1321 acgctgttaa taaagctaaa gctggcaagg ctggttcttc aggtggtgcg ctaaataaag
     1381 tcggtagagt tgtcctttat gctgagttgg taaattttgc tcttgataat ttaaaccctg
     1441 aagcagctaa gaatccgaac ttacatcaat caggtattgg cacagaaaca aacttcaacc
     1501 ctgaatacaa aggtgattca tctaagccaa tagttctatc gctgactggt ttaatggtgc
     1561 aactaataaa attgctaact tccaagaacc gatgaataac ttcgtaaata acacacaatc
     1621 aactccatca gctatcacag gtgacatggt ta
//
LOCUS       DAEEGA010000265         1603 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEEGA010000265 DAEEGA010000000
VERSION     DAEEGA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.270"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(335..481)
                     /locus_tag="KLH46_004350"
                     /pseudo
     CDS             complement(335..481)
                     /locus_tag="KLH46_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            535..717
                     /locus_tag="KLH46_004351"
     CDS             535..717
                     /locus_tag="KLH46_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HBE0380733.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(818..1147)
                     /locus_tag="KLH46_004352"
     CDS             complement(818..1147)
                     /locus_tag="KLH46_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBE0380734.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1319..>1603)
                     /locus_tag="KLH46_004353"
     CDS             complement(1319..>1603)
                     /locus_tag="KLH46_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380735.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          437 a          368 c          376 g          422 t
ORIGIN      
        1 atagcaatgt ctgtcgcaca acaaccacaa tgtcacaaat aaaaaatgag aataaccagt
       61 tccctgaagg cgacgtagcc cggtacaaga gtaacccacg ttagcgcttg gcgacggcgg
      121 aacaaagctg attcgcgacg taagaaagtg gcaggctcat atgacggacc tctgtaatgc
      181 ggtatccaac ccgcgaatat cagcatgatt caccgtcgga tttactgcta cgtcgccctc
      241 agggaattaa acctaaaaag aaaatcgtca aaattagaga ttccggtttg acacggaaag
      301 tcatatcagc gtcaacggag caccgtattg acgcttattt attggtgaga actacgttcc
      361 atggcaggag ttcgtcaaca cggttggagg gccattcagg cagtatgctc agaatatggc
      421 gcagatacgc ttccggatcg ataccgttca gacggcaggt gcctgctttt ccggcaacaa
      481 gcaggaatct cagcgctgaa gcaggaatta gaggtaaaaa cgccctaccg ggccatgaat
      541 cacccggtca ttggagtagt aaccaaagca gatttagcca gcatggaaca aatatctttg
      601 gtgaaaagtt ggttacggga agctggagcg cacaacgtgt tagtaaccag tgcagttaat
      661 aacaatggag ttacagaact ttttgccttg ctgcatacag aagaaggctg ttgttaattt
      721 cgattggttc tcagatgaga atgatgacgg tgatacacag ataagaatca atgagcaatg
      781 agggttgccg ggcaaccctc attgaataaa acgggaatta cttcgcttcg ccgtttttca
      841 tttcgcccat agctttcagc tttttggaga tatcgcggcg ttctttggag agctcggcat
      901 ttttgatgat gtaatcgtca acgcgatctt catagtcacc tttcatgctg gcgatgatgc
      961 cctggattgc ttcaacgctc atccctggct tgatgtaatc gctcaggttg tccagcaaca
     1021 ggacgcgctt ctggttgtca cggatctttt tctcaacgtc ctgaatttca cgttgcagtt
     1081 tgttcttacg acggaacaga cgaacaaatt ccagtacgtc ctggaatgaa ggcttggtag
     1141 tttccatttt tatacccctg ataatgtgag agtcggattc gtttaagcaa ccgctattcg
     1201 ttagggccaa cattactcat agctgccgca gatgacaatg cttttatccc ttttcactat
     1261 catacccttt atccttgctg aatcgaagca gcagcaagat gattctgaag ttcagaaatt
     1321 atttgcgcaa ggcccggcaa atcagattcg atagcaactc aagctgatgt gccgtttcca
     1381 tgttcagcca cacataacca tagattggtg tttccacgac tttcaggtca tggtggttat
     1441 cgagcaactg acgcagctct tccacagcat cgttcaattt ttccgtattg gcaaaaaccg
     1501 gctgcggatt accttcgtac agcgcatgaa caaggctcag cagtatttgc tgcatcatgt
     1561 gctgggtatc acaaagtttt tgcgcgttca ataacacgaa atg
//
LOCUS       DAEEGA010000266         1598 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEEGA010000266 DAEEGA010000000
VERSION     DAEEGA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.271"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            154..477
                     /locus_tag="KLH46_004354"
     CDS             154..477
                     /locus_tag="KLH46_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBE0380736.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            474..890
                     /locus_tag="KLH46_004355"
     CDS             474..890
                     /locus_tag="KLH46_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBE0380737.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            856..1380
                     /locus_tag="KLH46_004356"
     CDS             856..1380
                     /locus_tag="KLH46_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380738.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERRRHWTQELLSR
                     ELQRSLRPVKRKHK"
     gene            1377..>1598
                     /locus_tag="KLH46_004357"
     CDS             1377..>1598
                     /locus_tag="KLH46_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380739.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLS"
BASE COUNT          389 a          379 c          456 g          374 t
ORIGIN      
        1 gttcgtgggt ttttatacca ccaagcgaac cggcggtatg ctggtggatt ctcaggcgat
       61 taagctgatg aaaattgggg ctgcaacccg ccagaaagcc gctgcgtaat gcggtttttt
      121 gtgcccgcgt aatcgcgggc acaggaggaa aatatgctcc tgaaagaaga ggagattaaa
      181 tctcatctcc ggctcgatga tggtttgtac agtgacggcg attttctgaa actgctggca
      241 caggcggtac agaaaaaaac agagacatac ctgaacagga agttgtatgc accggaagag
      301 acgattccgg aagatgatcc tgacgggatg catctgactg atgacgttcg tctggcaatg
      361 ctgatgctgg tcagtcattt ttatgaaaat cgctcaacga ttaccgatgt ggagaaactg
      421 gaaacgccaa tgagtttcag atggcttgct ggcccttaca ggattgtccc gctatgaaaa
      481 tcaggcaaag tcagaccagt gccacttacc ttttaccgga tcccggagag ctgaatcgcc
      541 ggataaagat ccgccttcgt gtggatgaac ccaccgctga ttttggtacg gagccaacgt
      601 atccggagtc gtttgatgtc tgggcaaagg tggctcagcc aggcgctgcc gcttatcagg
      661 gctcagtgca gacagaaaat atcgttacgc attattttac gatccgtttc cggcacgaca
      721 tcacggcaga tcacgaagtg gtttattacg gccaagagta ccggatccgg cgaatacgtg
      781 acctgaacgg tcagcggcgt tttttattac tggaatgtga agaactgcgt acagcgcgac
      841 gacggggtga atgccatgaa tcagacagca tttttacacg tcgactttaa acaaccagag
      901 gagctggagt ttaatcgcgc ccgtctgcgt cgggcgtttg tgcaaatcgg gcgcgtttac
      961 atgcgtgatg ccagacgtct ggtgattaaa cgcggacgct ccggtccggg agagaacccg
     1021 ggatatcaga ccgggcgtct ggcccgctcc attggttatt acgttcccaa aaaaacgacg
     1081 cgtcgccccg gactgatggt gaaaatttcc cctgaccaga aaaacgggca ggggaatcgc
     1141 cgttttcctg aaggtgctcc ttattatccg gcgtttctgt attacggcgt tcgccattca
     1201 gcgtatggaa tggataaaaa ggataagcgg cagaagaagc accattcttc gactttccgg
     1261 ctggccccgc gtaataactt tatggctgac gttattgagc gtcggcgtca ctggacgcag
     1321 gaactgttgt cccgtgagct gcagcgttcg ttacgtccgg taaaaaggaa gcataaatga
     1381 aactgacaac gataattgcg gcactgcgtg agcgatgccc gcgttttgag gagcgtgttg
     1441 gtggcgcggc gcagtttaag gcgatccctg atgccggaaa acttcgcctg cctgctgcct
     1501 atgtggttcc ctctgacgat gcgccggggg aacaaaaatc acagaccgat tactggcagg
     1561 atttgactga aggcttctcc gtgattgtgg tgctcagc
//
LOCUS       DAEEGA010000267         1597 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEEGA010000267 DAEEGA010000000
VERSION     DAEEGA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.272"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(1..877)
                     /gene="pdeC"
                     /locus_tag="KLH46_004358"
                     /pseudo
     CDS             complement(1..877)
                     /gene="pdeC"
                     /locus_tag="KLH46_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            1306..1587
                     /locus_tag="KLH46_004359"
     CDS             1306..1587
                     /locus_tag="KLH46_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HBE0380740.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          467 a          337 c          359 g          434 t
ORIGIN      
        1 gttttgatat cgattattgg ctggtaataa agacaaagtt gatgtttttc gagggcgcgt
       61 tgcaatttac gtttgggaga taaatagttt tgtcgaatac gtagccagag taatagcaga
      121 acaagactcc ccaaaatacc ggcgggcaac gcaaaaataa gatgattata aaaatgggtt
      181 ataagacgtt gatatgaagt cgcaacaatg gctgcaattg ggcgttttgt cgaataaatt
      241 gtcgcatata aatagccatt tctttgtacg gttaaatcct tcaaatgaat cagcggagaa
      301 aacgttgctg ccgaggcctc tttgcttaac gagaaaaagg ttttcgtcac cgtatcatac
      361 acaccccatt gcaatgtcgg gtcatcagac atcacttcac tccagaagag agggttgata
      421 accgccatat aatttccccg ctgcatatag gtcattttat agccagagaa aaaaggcgta
      481 tcgcggtaat aatagataga aacgttaggt tcacgcttat aatcggccgg tgcaatcgta
      541 tagccgttta caggcgctat cagcgatgag cataaaaaat ggttatcacg aattcattaa
      601 tataaagata gccacgaata atattcaaca ttcgcttttg atgggctgga gtgcaaactt
      661 gcccctgata gcgttcagct gcatcgctca ctaaatctgc ctggcgaatg accagctcgg
      721 atttatccag tgccagttga gcaaaggttc ggagctgatt attcacttct gattttgccc
      781 aaaggaaggc aatccatagc gaaagaatga tgggaaagag aactaaaaag ataatgcccg
      841 gcaacgccag taattggtgt cgtgcacgat gactcattgc tatcaatttc ctgcctgttt
      901 atacccagcc attgcaaggt gaaaccctgg ctaccgacgt ctttagataa atcttacggt
      961 ataaatagca cttttgcctg atttgaaatt gatacctttt taatcgtcac ctggatgaaa
     1021 ataattcatt gatattcaaa attaattttc tacaggaata cagaggcggc gggaagatat
     1081 gcccttaatg ccactgaaaa accacataat ttgtacagca ataaaagtca cggcctaata
     1141 tggctaacaa acaatcaatt gtgatatagt tcacaaaatt aatgaaacaa acagagtgtt
     1201 tcatttttgt gttctgcaag ccccgcgata gccagagtgt accacgcctc ccgtgaacaa
     1261 cgccgcgctg tccagggcat cggctctttt cagaggaatt gtttgatggc taccctcacc
     1321 actggcgtgg ttcttcttcg ctggcaactt cttagtgccg taatgatgtt tctggccagc
     1381 acactcaaca tccgttttcg tcggtctgat tatgtcgggc ttgcggtgat cagcagcggt
     1441 ctgggcgttg tttccgcctg ttggttcgca atggggttgc ttggcatcac aatggcggac
     1501 atcaccgcta tctggcacaa catcgagtcg gtgatgatag aagagatgaa tcagacaccg
     1561 ccacaatggc caatgatttt gacctgatac gtagaag
//
LOCUS       DAEEGA010000268         1597 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEEGA010000268 DAEEGA010000000
VERSION     DAEEGA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.273"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..636)
                     /locus_tag="KLH46_004360"
     CDS             complement(<1..636)
                     /locus_tag="KLH46_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBE0380741.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1322)
                     /locus_tag="KLH46_004361"
     CDS             complement(750..1322)
                     /locus_tag="KLH46_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0380742.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGKIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          475 a          336 c          318 g          468 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aattttaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtatttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAEEGA010000269         1582 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEEGA010000269 DAEEGA010000000
VERSION     DAEEGA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.274"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            1..253
                     /locus_tag="KLH46_004362"
                     /pseudo
     CDS             1..253
                     /locus_tag="KLH46_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..1489
                     /locus_tag="KLH46_004363"
     CDS             746..1489
                     /locus_tag="KLH46_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE0380743.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCTN
                     FVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQEL
                     CGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERVM
                     DRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1504..>1582
                     /locus_tag="KLH46_004364"
     CDS             1504..>1582
                     /locus_tag="KLH46_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBE0380744.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGR"
BASE COUNT          384 a          358 c          491 g          349 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gctaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tccgctccgg catccagccg ttgcaccgga agtgttcgtt
      961 tgcgaattac cgggcgcaga acgacggtca gcgacatgca ctgagtcagg cgaaatccat
     1021 agctgacgaa ctgatgacag gctgtacgaa ttttgtgttc agcggtaagc ccggcaccgg
     1081 aaaaaatcat cttgcagccg cgattggcaa ccggctgatg gcgaaggggc gtagcgtgat
     1141 tatcgtcacc gtgtccgatg tcatgagcgt gttgcatgag agctacgaca acggcaaatc
     1201 cggtgaaaaa tttttacagg agctttgtgg tgttgacctg ctggtcctgg atgaaattgg
     1261 catgcagcgg gatacgaaaa acgagcaggt ggtactgaac cagattgttg atcgccggac
     1321 ggcatcgtta cgcggtgtgg ggatgctgac aaatattaac catgcagcga tgaatacact
     1381 tctcggcgag cgggtgatgg atcgcatggt catgaacggc gggcgctggg ttaattttaa
     1441 ctgggagagc tggcgtccga atgttagcca ttcgagggtt gttaagtagt ttcaggagga
     1501 tttatggcga aaccttttac tcccgaacag cgggaagaac tgaagacgcg aattgtggaa
     1561 ctcgtgcatc aggacggtcg gg
//
LOCUS       DAEEGA010000270         1573 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEEGA010000270 DAEEGA010000000
VERSION     DAEEGA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.275"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(373..447)
                     /locus_tag="KLH46_004365"
     tRNA            complement(373..447)
                     /locus_tag="KLH46_004365"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(412..414),aa:Gly,seq:tcc)
     gene            complement(451..526)
                     /locus_tag="KLH46_004366"
     tRNA            complement(451..526)
                     /locus_tag="KLH46_004366"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(491..493),aa:Thr,seq:tgt)
     gene            complement(529..605)
                     /locus_tag="KLH46_004367"
     tRNA            complement(529..605)
                     /locus_tag="KLH46_004367"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(569..571),aa:Arg,seq:tct)
     gene            complement(607..682)
                     /locus_tag="KLH46_004368"
     tRNA            complement(607..682)
                     /locus_tag="KLH46_004368"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(647..649),aa:Ile,seq:cat)
     gene            complement(859..1548)
                     /locus_tag="KLH46_004369"
     CDS             complement(859..1548)
                     /locus_tag="KLH46_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBE0380745.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
BASE COUNT          415 a          417 c          332 g          409 t
ORIGIN      
        1 agaaaaagcc agttcttcgc ccctaaataa cagcatgatt ccagtccatg caaaatctga
       61 tttccagctc acacataccg agcgctgtac aaaaaaccgc cagccaaggc agttaagagt
      121 gtgttcccgg ggtttgctta gaatattttg taagttgtcc gaagagatat ttacaacacc
      181 agaatgatga atcatcagtc ccctgccaga ggttcactac gtaacctgac cgacaatgca
      241 ccattctggt gctgaaaaaa gagcactgga actgcaacaa aacaacatca cagaacagga
      301 gatggtaagg agtcagacat tcccacacaa tattgtgtca gtgcaaataa caaccttcgt
      361 ctcaaatctc gctggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg
      421 aggtaatagc cattatacga tgcccgcata tggtgccgac taccggaatc gaactggtga
      481 cctactgatt acaagtcagt tgctctgcct gctgagctaa gtcggcgctg gcccaccacc
      541 gaggactcga acctcggacc gtcaacttag aaggttgatg ctctatccgg atgagctagt
      601 ggtggttggt ggcccttgct ggacttgaac cagcgacctg gcgattatga gtcgctcgct
      661 ctcaccactg agctaaaggg ccggaggcag aataataacc atatgtcatc acatctgcaa
      721 actcatctga ccaccagcac gtttaacgtc ctgtgccatt tttcaggcat aaaaaaaccg
      781 ctcggcggcg ggttttgctt actttaccat cgcgtacaaa atcggcaaag tatcagattt
      841 gcatgagata tatgcctttt aatctacttt tgcaatactt tgctttgaaa atgccgcttt
      901 ttgttctgaa cgtgttctca tcacaaacaa taaagcctca ctatccagtc ggtgaaaaat
      961 gtgtttcatt acaacccagt gaccagtaaa tgttttggac cagtttttgg ttgttactcc
     1021 cgccagtaat gccagctcct gatattcata accttcccca ccaaaaagtt ctgcttttac
     1081 cgcctgcgcc gccagccaga ttaatttttt caggcgttcc tgtgttttct ctgcaatttt
     1141 cctagtgcca gaatgagcct taaactcttc ccacgcccag tgggttatgg tgatctgata
     1201 ctcccagcaa atacttccgc tgtaacacca caacaaccag gctttatagt gctcttcaag
     1261 agacagaaca gcccgccgcc acgatgatgt cgaaaacgca acctgactga ccagaggaat
     1321 tgacgccccc ttcgccagtg attgttttcc cgggattggt ggattatccc gcgttatcat
     1381 ttttccggtc acctcatcgc ggtaccggat ttttttacgc ctgtaacgcc ctgtatcgaa
     1441 catggcattc tcctgccagg cttcaagctg accttttgtc gccccactca aatcagcggt
     1501 ggcgataatg agctgctcac gaacaaactg taaatactgg ttattcatgc acactccagc
     1561 tctgtgattt tta
//
LOCUS       DAEEGA010000271         1502 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEEGA010000271 DAEEGA010000000
VERSION     DAEEGA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.276"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(149..631)
                     /locus_tag="KLH46_004370"
     CDS             complement(149..631)
                     /locus_tag="KLH46_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0380746.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(622..924)
                     /locus_tag="KLH46_004371"
     CDS             complement(622..924)
                     /locus_tag="KLH46_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBE0380747.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1196..1468
                     /locus_tag="KLH46_004372"
     CDS             1196..1468
                     /locus_tag="KLH46_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380748.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          429 a          374 c          322 g          377 t
ORIGIN      
        1 gatcgaaagc tgattttcag gaaccgtttt gacggcttcg gctttaaact ccgtagagta
       61 acgttgggtt tttctgctca ttattggctc cttctgatgc cattctattt caggaaggag
      121 tgtccgttaa actcaggcta cctcagactc agactttata aaacaaggta ttaggtgcca
      181 caactgactg aacaaaacca aaacgctcat aaaatgctct ggcgtcatca tcaattgcat
      241 gtaccataac agccttaatg ccaacctgat cagccagatt gacaacccgg tgaatggcat
      301 cacttagtag ccatttaccg aaaccatgcc cctgggtaca aacatcaaca gccagacgcc
      361 ctaacagaac aacagggaca ggattgggca tgttatgccg caaactgcga ccaagctcgg
      421 catgagtgac ggaaccagta gctaatgagt agtatcccac aatacgtaaa gtatcctcca
      481 agcaaataac gaacgtgcga gaagcattga gcatctggtt tttcatagcc cgaccacgta
      541 accagttact tagcacctca tttccacagt caaatggctg aagtatatgc tcatccgtca
      601 acaatgctgg cgcggtgaca tttatttcca ttcaggcttt accgccatca gacgttcacg
      661 cccttctgca ttctgcacag gagcttccag ttgcgtaata aacgaatcat aacgttcgtt
      721 atccagcaca aataaacgtt gatccaggat aatgttttta gcttcctggt acgccagttc
      781 catgatgaaa tcggtacggt tcttattgac caagctcgca gcataatcaa taacggctcg
      841 ttcctcatca gttgcccgga tgttaatctg atgttctcgc ggtgttttac ggacgcttgc
      901 agctgtagac ataaaaacct ccattggtaa caatttgcta tgcaattaat gttatcgcaa
      961 atggtaacag aacgctatac agaatcactt acgggaaaag cgatcccctg ataaaaacaa
     1021 tggcagagac ctgaacattg tgacgatgat ctgtgtcggc gcacaaatca tgtctgatta
     1081 cggccgttta atgtgatttt gataatgaaa tgcctgaacc acggcagcct ggcggctgca
     1141 aatcggggtg tggcagaata tgagctttca gagtaaccat ccagaatgag ttcccatgtc
     1201 acacaacctg gagcaccaga aagtccacac cagaatggtg aaagaggttc tgaaagccgt
     1261 cgccagagcc aataatcacc cttataagtc cgtatttgca gactttatca ccggtcatcc
     1321 ctcctgcacg gtctgtttct gggaaacctt ccacaaaatg tacccggatt caccgtacga
     1381 atacgtcacg ttctgccata cctgccgtcg ttttgattta tacgaaacag aagcggagat
     1441 gaaggcggat gacccgaagt ggtggtgatt ttcagccccg accgctgaaa cgcctgttca
     1501 ca
//
LOCUS       DAEEGA010000272         1481 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEEGA010000272 DAEEGA010000000
VERSION     DAEEGA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.277"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..243
                     /locus_tag="KLH46_004373"
     CDS             <1..243
                     /locus_tag="KLH46_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBE0380749.1"
                     /translation="AENDALRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVEL
                     SPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(332..844)
                     /locus_tag="KLH46_004374"
     CDS             complement(332..844)
                     /locus_tag="KLH46_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HBE0380750.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1039..>1481
                     /locus_tag="KLH46_004375"
     CDS             1039..>1481
                     /locus_tag="KLH46_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380751.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVA"
BASE COUNT          443 a          338 c          362 g          338 t
ORIGIN      
        1 gctgaaaatg atgctcttcg gcgcaagctt gataatggtg gtcgggtgct cgtcaaagga
       61 aaatgccctg tgccatcctc agccgaaacc tccagcgcct ccggcatggg caatgatgcc
      121 accgtcgaac tctctccagt tgctggacga aacgttctcg gtatccggga cggaatcatc
      181 agcgaccaga cagcattgag aatgcttcag gagtacatca ggatccaatg ccttgggggg
      241 tagcggtaat tttactcatt atccttcaaa tcagattctg ttgtcagagg aatgggggaa
      301 gctggattcg caagagcttt ttatgggcag tttagctaaa aaaaggcaac acgaagctaa
      361 aactaccaac ataaagtgct gaccagctag cagctgaaat cagactcact gtatacactt
      421 tcactggatg aagttgcaac attcctgcca ccaaaggagc aatgtaacgc agcacggcga
      481 taaagcgcga agtgaaaagt attgacacag agttattttg caactgaagg cgaacccgtt
      541 ctattgtatt ggaacggctc gatattatac atgcaagttg agggatatgc ccaattatta
      601 taccaagatg gtaattaacg atagtaccgc accatgcgcc actcatgacc gtgattccag
      661 cttcccatgg tgaaagagtc gtcttgctga ctgtaattac agtggccatc ataattgatg
      721 ctggtggcaa tatcgccgaa atgagtaaag tggattttgt gaacgctatt ataaaaagaa
      781 gcccccatag gcgatatgga tgaagtacaa aatagttcat caaagcattt atccactcca
      841 tcaatgtctt tccttctgtg aatttttctt tgctatttaa ccgtgatacg acttaattta
      901 ggcttaaaca ccttacattt acaaggtact cctgtggggg gccctgccac ggagcgtcgc
      961 aggagtgagt gatgaggaag aattaaagca acctattgtg acaggcagcg ataacagtaa
     1021 atcacaggaa gcataatcat ggcaaaaccg gactgggagg ccatcgaatc ggcataccgg
     1081 gccggagtcc ttagcctccg tgatataggc gagaaatacg gcgttacaga aggggctatc
     1141 aggaagaggg ccaaaaagct tgattgggca cgcagtggcg gtacgcaggt ttgcaaaaat
     1201 ggtacgcaaa aaaggaaagt gcgtaccagc agaaagcctg ccattactgg ccttacacaa
     1261 aaaagtacgc aactaaaaac agaatctaca ccggatacga aaccgatacg cggaatgcgt
     1321 accgatcccc cgactaaccc attccaaccc ggtaaccagc aggcattaaa acacggtggt
     1381 tatgcccgtc gccttctgct caaagatgag gtgatagagg acgctaaagc gttgacgctc
     1441 gaggacgaat tatttcgcct tcgtgctaac aaccttgtcg c
//
LOCUS       DAEEGA010000273         1406 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEEGA010000273 DAEEGA010000000
VERSION     DAEEGA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.278"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..169
                     /locus_tag="KLH46_004376"
     CDS             <1..169
                     /locus_tag="KLH46_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBE0380752.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGTL"
     gene            154..792
                     /locus_tag="KLH46_004377"
     CDS             154..792
                     /locus_tag="KLH46_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HBE0380753.1"
                     /translation="MGDSVTPETLGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1020..>1406
                     /locus_tag="KLH46_004378"
     CDS             1020..>1406
                     /locus_tag="KLH46_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380754.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          348 g          394 t
ORIGIN      
        1 gtccgctatt gccagtacac ctcacccgtc ctggcgacgt atctgtaaga cactcataaa
       61 gaatgatttc tggtgccgga ctctctcctt cagtccgaac aaaccccggc actatgaacg
      121 ttacctgcag cgtatgaagg aaaggaggaa ggaatggggg actctgtgac accggagaca
      181 cttggcaaca tgattcgtca gtatttcagt caggtacgaa ctgaaaaaga gagcatacag
      241 gctctgaatc atctgcgcag agtgcttcat gaggtcagtc cttttgcaca ggagcctgtg
      301 gactgtgtgt tgtgggtgaa agcggatgag gtggtggcca acgattacaa ccctaatgtc
      361 atggcactag gtgagaaaaa actactgaaa cagtctctgg agaaagatgg tttcacacaa
      421 ccggtggtgg tgtcagagga gaagaatcat tatctggtgg tggatggttt tcatcgtcag
      481 ttactggggc gggaagcaga tgcaggaaaa cgcctgaggg gctggttgcc tgtagcctgt
      541 attaatccgg agagaaaggg acaggccgca cgtattgccg cgaccattcg acataaccgg
      601 gccaggggga aacaccagat cactttaatg tctgatattg tcagggatct ttcacgtctg
      661 ggctggacga atgaacgtat tggtacagag ctgggaatgg accaggatga agtgctgagg
      721 ctgaaacaga ttagcgggct gacagagttg tttcaggaag aggatttcag ctcggcttgg
      781 acggttcgtt gatgttgtta aatatgggcg gtatttctga aagttgatac tttgggtaga
      841 aaaaatgata ccgaaaacta tggaggatgt gcttgtcagg tgactggaca ccataggtct
      901 taggctttga taacgggata agatgagcgg cttttctcat accatcaggt gtgatgatgg
      961 cgcagattta caaaatctga ttgattctaa agatgtataa gtaaaaatca agctttgata
     1021 tgaaaaaaca gatatttatt aataataaac ctcctgtagt cccttactca gggacgcacg
     1081 cgaaaatatt taaatacatt gaaatccctc tgccgttttt ctactttatc tatacatcag
     1141 gcgaaccatt ccacatatct gtccaaaata ctgttattta tgtatcaaaa tacaatggta
     1201 tatttattaa taaattagtt cctttttctc ttttatttga cagggatata tctgttcttc
     1261 aaagaagaga tatttgtgtt gtgcgattta cgtcagagga aataagtgaa cataacgttt
     1321 tatttgatca tgacattgaa cgattaaaaa aaatttccaa agctcagctt atatctcccg
     1381 attatgtttt gattgatttt tctagt
//
LOCUS       DAEEGA010000274         1334 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEEGA010000274 DAEEGA010000000
VERSION     DAEEGA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.279"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..171)
                     /locus_tag="KLH46_004379"
     CDS             complement(<1..171)
                     /locus_tag="KLH46_004379"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380755.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASL"
     gene            308..>1334
                     /locus_tag="KLH46_004380"
     CDS             308..>1334
                     /locus_tag="KLH46_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HBE0380756.1"
                     /translation="MEDASTTKKGIVQLSSATNSASESLAATVKAVKVVMDETNKKAP
                     LNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAALVDSSPDALNTLNELAAALGND
                     PNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAK
                     STVAAVIEYLGLQETVNKADNAVQKTGDTLSGGLTFENDSILAWIRNTDWAKIGFKND
                     SDADTDSYMWFETGDNGNEYFKWRHRLAGGQLKELMNLKWDSLNILVNAVINGCLGIG
                     TTNALGGNSIAFGDNDTGLKQNGDGLLDVYANGQHVFRFQNGVAIAFKNIQAGTARKF
                     TLSSANNS"
BASE COUNT          352 a          332 c          380 g          270 t
ORIGIN      
        1 cagggatgcc ggcacatctg ccagtgaggc ggcaaccaat gcgactgatg ctgcaggctc
       61 agcacgcgca gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc
      121 ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc
      181 ctctaaggcg acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc
      241 agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt
      301 cgtatctctg gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac
      361 caacagtgca tccgagtcac tggccgcgac agtgaaagcg gttaaggtgg taatggatga
      421 aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc ggaacgccaa ccacacccac
      481 tgcgccaaag gggactaata atactcagat cgccagtacg gcttatgtta tggccgcgat
      541 tgctgccctc gtggactcgt cgcctgatgc actgaacacg ctgaatgaac tggcggcggc
      601 gctgggcaac gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc
      661 gaaagatgcc actctgacgg cgctggcggg gcttgctact gcggcagaca ggtttccgta
      721 ttttacgggg aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct
      781 tgcgaaatcg accgttgccg ccgttatcga atacctcggt ttacaggaaa cggtaaacaa
      841 ggctgataac gctgtccaaa agacaggcga taccttgtcc ggtggactta cttttgaaaa
      901 cgactcaatc cttgcctgga ttcggaatac tgactgggcg aagattggat ttaaaaatga
      961 ttcggatgca gacaccgatt cctacatgtg gtttgaaaca ggtgacaacg gcaatgagta
     1021 tttcaaatgg agacaccgcc tcgctggcgg ccagctcaaa gagctgatga atcttaaatg
     1081 ggattcacta aatattctgg ttaatgccgt cattaatggt tgccttggca ttggtacgac
     1141 gaatgcgtta ggtggaaatt caattgcttt cggggataac gataccggcc tgaaacagaa
     1201 cggcgatggt ctgctggatg tttatgcgaa tggacagcat gtatttcgtt ttcagaatgg
     1261 cgtggctatt gcttttaaga atattcaggc cggaactgcc agaaaattca cgttatccag
     1321 cgccaacaac tcca
//
LOCUS       DAEEGA010000275         1271 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEEGA010000275 DAEEGA010000000
VERSION     DAEEGA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.280"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            482..>1271
                     /gene="uacT"
                     /locus_tag="KLH46_004381"
     CDS             482..>1271
                     /gene="uacT"
                     /locus_tag="KLH46_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBE0380757.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGLMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAV"
BASE COUNT          294 a          292 c          279 g          406 t
ORIGIN      
        1 gacagcctat acattccgca gcttcagcag cgataaactt attcatttgc atccctttca
       61 tttaatgagt tatgtcttct caaatttcgc gatgcaataa acgggcatat attaggtagc
      121 atgaccgttt taattataat tttgcgactg agttcaaatt attgcccctc aatctatgcc
      181 gctttcatta tttcaacatt gccgatcaaa agagcgctat ccagataaaa acgattaaat
      241 gcgagagtgc gacatgccag aatgattaat gaaatatcaa tatgagaaaa ttacagataa
      301 ttccattgtc aggttctctt gtcattttcg tggtttaagc acgattatgt gattgcctcc
      361 gcaattcctg tctctaactc cccttcctcg caaaaactgg cactccacga gcatgtgttt
      421 agacagtttc attaacgtaa acggttgctt tttattctgg cgggcgaaag gagaaacact
      481 gatgagcgcc atagattccc aacttccctc atcttctggg caagaccgcc caactgatga
      541 ggttgaccgc atattatcac caggaaagct gatcatactc ggtctgcaac acgtccttgt
      601 catgtacgca ggtgcagtcg ctgttcctct tatgattggt gaccgactgg gcctctcaaa
      661 agatgctatt gcgatgctca ttagctcgga tctcttttgc tgcgggatcg tcacattatt
      721 gcaatgtatc ggtatcggcc gctttatggg gatccgcctg ccggtgatta tgtcggtgac
      781 ctttgctgct gtaacaccaa tgatagccat tgggatgaac ccggatatcg gcctgctggg
      841 gatatttggt gccactatcg ccgcgggttt tatcaccaca ttattagcgc cacttattgg
      901 tcgcttgatg cctttattcc cgccactggt taccggtgtg gttattactt ctattgggct
      961 tagcatcatt caggtgggta ttgactgggc cgctggaggt aaagggaatc cgcaatatgg
     1021 taatcccgtt tatttaggta tctcctttgc cgtcttaatt tttatcttgc tcattactcg
     1081 ctatgcgaaa ggattaatgt ccaacgtcgc cgtattactg gggattgtat ttggcttttt
     1141 actttcgtgg atgatgaatg aagtcaattt atccgggcta catgatgcct cgtggtttgc
     1201 gattgtcacc ccgatgtcgt ttggtatgcc gattttcgat cccgtttcca ttctgaccat
     1261 gactgccgtg t
//
LOCUS       DAEEGA010000276         1266 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEEGA010000276 DAEEGA010000000
VERSION     DAEEGA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.281"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..340
                     /locus_tag="KLH46_004382"
     CDS             <1..340
                     /locus_tag="KLH46_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380758.1"
                     /translation="PVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARY
                     KYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYY
                     RPLQKLINGT"
     gene            364..908
                     /locus_tag="KLH46_004383"
                     /pseudo
     CDS             364..908
                     /locus_tag="KLH46_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            932..>1266
                     /locus_tag="KLH46_004384"
     CDS             932..>1266
                     /locus_tag="KLH46_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBE0380759.1"
                     /translation="MSVVISGALTDGAGISMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWHNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVA"
BASE COUNT          357 a          271 c          332 g          306 t
ORIGIN      
        1 gcctgttgtt gcagagcttg gcgatgattc aggctggcat tttttcagcc agagaaaccc
       61 ggataacagc atagtgttca ctgttaacgg acaggtaatt ccgttaaatt acggaaactt
      121 cgatgcccgc tataaatatc gaacagaggg ggtacaggat gtacggtatg gccatgaaat
      181 gtattacagc cccggcagta acaccgtttc gtggagattt tgcgcacctt cgggacacgg
      241 gctgtcaggg atggcgatat cggataccgg ccgtaactca gcggataacg ttgacggtgt
      301 gtattaccga ccactgcaaa aactgattaa tggcacctag tataacgtag cgagtattta
      361 acaatgttgc atttaaaaaa tattactacg ggtaatccga aaacagcaga acaatatcag
      421 atgacaaaac gatattcggt cacctggctt ttttcagaag acggaaaaaa ctggtatgaa
      481 gagctgaaga atttcgccag gacacaatta aaataactta caccggagac ggtcgcgtgg
      541 tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt attgaagttc
      601 ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac cgcaatgatg
      661 aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt ctggctcagg
      721 tcagtgcccg gacaggggaa tgggaagaag acctgttgct ggggctaatc agcgacgaag
      781 accgggaaaa actgaaagcg taccgcattt acgcgaaatc gctgcaggcg atggatttca
      841 gtgtcatcac tgataaaacc tcatacaacg ccattgaatg gcccgcttct ccggaaattt
      901 cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag gtgcgctgac
      961 tgatggcgca ggtatctcca tgtccggata ccatattatt ctgaaatccc gggtaaacac
     1021 cccggaagtg gtgatgcaca ctgttgctga tgtgatgaca ggaaacgatg gtgaatactg
     1081 tttccatgcg cggactggaa aatatggtgt gtatctgaaa caggactggc acaacgagta
     1141 caacgttggc gacattgctg tatatgagga ctcaaagccc ggtacgctga atgactttct
     1201 gattgctcct gatgagggcg acctgaaacc agatgtcgtc aaacgctttg aggaaatggt
     1261 ggcgca
//
LOCUS       DAEEGA010000277         1259 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEEGA010000277 DAEEGA010000000
VERSION     DAEEGA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.282"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            34..435
                     /locus_tag="KLH46_004385"
     CDS             34..435
                     /locus_tag="KLH46_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBE0380760.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="KLH46_004386"
     CDS             447..818
                     /locus_tag="KLH46_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0380761.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGIFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1259
                     /locus_tag="KLH46_004387"
     CDS             805..>1259
                     /locus_tag="KLH46_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0380762.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPV"
BASE COUNT          299 a          281 c          390 g          289 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatcttgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccggga tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtgga
//
LOCUS       DAEEGA010000278         1225 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEEGA010000278 DAEEGA010000000
VERSION     DAEEGA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.283"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..781)
                     /gene="ipaH4.5"
                     /locus_tag="KLH46_004388"
     CDS             complement(<1..781)
                     /gene="ipaH4.5"
                     /locus_tag="KLH46_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HBE0380763.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLST"
BASE COUNT          349 a          220 c          264 g          392 t
ORIGIN      
        1 gagtcgacaa tggattccct gaaatgttaa cattcgtaca aatgggcaat gtgattatat
       61 tttcgggtaa tgaagttagt aggttattgg aaacatccag tgccaatagt tcttgcggta
      121 atctggataa tctgcgtagt tgattttcac ctaccttcaa atattttaag ctctgaggaa
      181 attctggtag ttcctgtagt ctgttgccgg aaacaaagag ttcttgtagt ctatgaggca
      241 attcaggcaa gacagtaagg cgattacctt caatacgtaa tattgttagc gttgatggaa
      301 gctctggtaa cgtctccaga ctattaaaac gtgccgagag tgattgtaaa tatggtggta
      361 atgatggcag acatgacaaa tttcgattga atgaaatatt aagcttaatc agttgagatg
      421 gaagtgtcgg gagtatattc aagttgttac catttacatg aagttctgtc aaaagcggag
      481 aattttcagg tagggagatt aactcattgt tggaaatatt aagttcacgt atatgcaaag
      541 ggagtggtgg taaagaacgt agtttcagta agcttaaatt gagaacaggc tcccgggtat
      601 ccagacatat tttcaataat cgaaccgcct ggatgcgatt ttctgttgtt gttccttccc
      661 tagcccagtc atcccagatg cggtggtaat ctctggtaca ctgttcttct accgataaac
      721 gagatataca tgataagcca caaagggaac gaaaaaaaga atgattgttg atcggtttca
      781 tttattaacc cctcactatt catatcttgg tctattaatg caaataaaac gagaaagtct
      841 ttaaaaaaac aatcctagaa ctttatcggc gttttcattc cggctggagg aatatgttcc
      901 tgtctttcca gtaaccgtcg attgttatat cagtctaccc atgttaatgt gaccagttct
      961 actttcatcc agtttggatt gatctttatc ccctatattt tcaaacaatt tttatcattt
     1021 aactcattac tgtctcgata ttcccatagc aaacgatctg gttggttatc ccctcaaaaa
     1081 agtcagttgc acgccgtaaa tccctgccgg tattgagcga ggatttacgg gttgttgtta
     1141 aggtccgtaa tcgggtcaca gtttttccgg agtcaatccg gtctgcggtt tatgcttatg
     1201 cgacgtgatt atgaatggtg cagtc
//
LOCUS       DAEEGA010000279         1202 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEEGA010000279 DAEEGA010000000
VERSION     DAEEGA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.284"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..>1202
                     /locus_tag="KLH46_004389"
     CDS             join(<1..299,299..>1202)
                     /locus_tag="KLH46_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HBE0380764.1"
                     /translation="PEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQLFLWR
                     KQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYGRGKK
                     VDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHH
                     VIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRLMRQNALLLERKPAVPPSKRAH
                     TGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQD
                     VMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRAC
                     NGLSDNRCL"
BASE COUNT          295 a          294 c          365 g          248 t
ORIGIN      
        1 gccggagaaa cgcagacggc gtaccacaca ggaaaagatc gcaattgttc agcagagctt
       61 tgaaccgggg atgacggtct ccctcgttgc ccggcaacat ggtgtagcag ccagccagtt
      121 atttctctgg cgtaagcaat accaggaagg aagtcttact gctgtggccg ccggagaaca
      181 ggttgttcct gcctctgaac ttgctgccgc catgaagcag attaaagaac tccagcgcct
      241 gctcggcaag aaaacgatgg aaaatgaact cctcaaagaa gccgttgaat atggacgggc
      301 aaaaaagtgg atagcgcacg cgcccttatt gcccggggat ggggagtaag cttagtcagc
      361 cgttgtctcc gggtgtcgcg tgcgcagttg cacgtcattc tcagacgaac cgatgactgg
      421 atggatggcc gccgcagtcg tcacactgat gatacggatg tgcttctccg tatacaccat
      481 gttatcggag agctgcccac gtatggttat cgtcgggtat gggcgctgct tcgcagacag
      541 gcagaacttg atggtatgcc tgcgatcaat gccaaacgtg tttaccggct catgcgccag
      601 aatgcgctgt tgcttgagcg aaaacctgct gtaccgccat cgaaacgggc acatacaggc
      661 agagtggccg tgaaagaaag caatcagcga tggtgctctg acgggttcga gttctgctgt
      721 gataacggag agagactgcg tgtcacgttc gcgctggact gctgtgatcg tgaggcactg
      781 cactgggcgg tgactaccgg cggcttcaac agtgaaacag tacaggacgt catgctggga
      841 gcggtggaac gccgcttcgg caacgatctt ccgtcgtctc cagtggagtg gctgacggat
      901 aatggttcat gctaccgggc taatgaaaca cgccagttcg cccggatgtt gggacttgaa
      961 ccgaagaaca cggcggtgcg gagtccggag agtaacggaa tagcagagag cttcgtgaaa
     1021 acgataaagc gtgactacat cagtatcatg cccaaaccag acgggttaac ggcagcaaag
     1081 aaccttgcag aggcgttcga gcattataac gaatggcatc cgcatagtgc gctgggttat
     1141 cgctcgccac gggaatatct gcggcagcgg gcttgtaatg ggttaagtga taacagatgt
     1201 ct
//
LOCUS       DAEEGA010000280         1166 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEEGA010000280 DAEEGA010000000
VERSION     DAEEGA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.285"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            119..>1166
                     /gene="cadB"
                     /locus_tag="KLH46_004390"
     CDS             119..>1166
                     /gene="cadB"
                     /locus_tag="KLH46_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HBE0380765.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASAILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASD"
BASE COUNT          248 a          258 c          299 g          361 t
ORIGIN      
        1 aatctttagg attaatcctt ttttcgtgag taatcttatc gccagtttgg tctggtcagg
       61 aaatagttat acatcatgac ccggactcca aattcaaaaa tgaaattagg agaagagcat
      121 gagttctgcc aagaagatcg ggctatttgc ctgtaccggt gttgttgccg gtaatatgat
      181 ggggagcggt attgcattat tacctgcgaa cctagcaagt atcggtggta ttgctatctg
      241 gggttggatt atctctatta ttggtgcaat gtcgctggcg tatgtatatg cccgactggc
      301 aacaaaaaac ccgcaacaag gtggcccaat tgcttatgcc ggagaaattt cccctgcatt
      361 tggttttcag acaggtgttc tttattacca tgctaactgg attggtaacc tggcgattgg
      421 tattaccgct gtatcttatc tttccacctt cttcccagta ttaaatgatc ctgttccggc
      481 gggtatcgcc tgtattgcta tcgtctgggt atttaccttt gtaaatatgc tcggcggtac
      541 ctgggtaagc cgtttaacca ctattggtct ggtgctggtt cttattcctg tggtgatgac
      601 tgctattgtt ggctggcatt ggtttgatgc ggcaacttat gcagctaact ggaatactgc
      661 ggataccact gatggtcatg cgatcattaa aagtattctg ctctgcctgt gggccttcgt
      721 gggtgttgaa tccgcagcag taagtactgg tatggttaaa aacccgaaac gtaccgttcc
      781 gctggcaacc atgctgggta ctggtttagc aggtattgtt tatatcgctg cgactcaggt
      841 gctttccggt atgtatccgt cttctgtaat ggcggcttcc ggtgctccgt ttgcaatcag
      901 tgcttcagct atcctcggta actgggctgc gccgctggtt tctgcattca ccgcctttgc
      961 gtgcctgact tctctgggct cctggatgat gttggtaggc caggcaggtg tacgtgccgc
     1021 taacgacggt aacttcccga aagtttatgg tgaagtcgac agcaacggta ttccgaaaaa
     1081 aggtctgctg ctggctgcag tgaaaatgac tgccctgatg atcctcatca ctctgatgaa
     1141 ctctgccggt ggtaaagcat ctgacc
//
LOCUS       DAEEGA010000281         1160 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEEGA010000281 DAEEGA010000000
VERSION     DAEEGA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.286"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..387
                     /locus_tag="KLH46_004391"
     CDS             <1..387
                     /locus_tag="KLH46_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380766.1"
                     /translation="LVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(388..1032)
                     /locus_tag="KLH46_004392"
     CDS             complement(388..1032)
                     /locus_tag="KLH46_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0380767.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAAMPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          296 a          263 c          277 g          324 t
ORIGIN      
        1 ctggtgctgg tggcaattct gattatgctg ccgactctcg gcatcggtga tatgaacaat
       61 ctctataaat ggctgttgaa tcttaactcg gtagtgatgc cgctgcgtta tttatgggta
      121 tttgttgcat ttattgcagt cgttcgcctg gcgcagaaat ataaaccaga atatgtcttt
      181 attcgtaata agcctctggc aatgaccgtc gggatttggt gttttgcctt taccgcctgt
      241 gcctgtttga cggggatctt cccgaaaatg gaagccttca ctgcagagtg gaccttccag
      301 ttggcgctga atgttgcaac gccgtttgtg ctggtaggat taggactgat attcccgctg
      361 ctggcgcgta aagcgaatag caaataatta ttgtggtcgg tgacgctgac tctggcagat
      421 ctgacccgtt tttacataat cgagaaatgc gcgcaagccg caggacatat actggcggtt
      481 tgggtaatag acttggaagc ctggacgctc tgtgatccag tcttccagta cacattctaa
      541 acgaccagta tcaaggtact ccttgatctg ttcatagagc agatacccga taccagcacc
      601 catcaaaacg gcctccagtt cagcatcaac atcatccaga atgatatttc ctgcgacagc
      661 aatttccagc tcctttgcaa attgccagtg gaaaggtttg ccgctgggat aacgaaaaac
      721 aacacattga tgattaagca gatcatgagg atggcggggt ttgccatatc ttgcaaagta
      781 ctctggcatt gctgctacac ataatttcac tggtgggccg atcgccacgc tgatcatatc
      841 tttttcgacg atgccactaa gtcgaacgcc agcgtcaaag ccctgttgga cgatatcgat
      901 aagagaatcg tcagttgtga gctctacttt aatatcagga tattcacgtg tgaaaccaac
      961 cagtaaaggc attaaaaata tacgtgcagc tacccgggcg gcattgattt tgagcgttcc
     1021 tgtcggtgtc agacgaaaat cattcatctc atctaacatg atctgaattt cgtcgaaagc
     1081 tgggcgcaat cgttcataga gattagaacc cgcttccgtt aacgatacgc ttcgggttgt
     1141 ccgattgaat aagcgaattt
//
LOCUS       DAEEGA010000282         1111 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEEGA010000282 DAEEGA010000000
VERSION     DAEEGA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.287"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            1..1019
                     /locus_tag="KLH46_004393"
                     /pseudo
     CDS             1..1019
                     /locus_tag="KLH46_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          272 c          259 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag c
//
LOCUS       DAEEGA010000283         1087 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEEGA010000283 DAEEGA010000000
VERSION     DAEEGA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.288"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..249
                     /locus_tag="KLH46_004394"
     CDS             <1..249
                     /locus_tag="KLH46_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0380768.1"
                     /translation="KNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            complement(367..808)
                     /gene="tnpA"
                     /locus_tag="KLH46_004395"
                     /pseudo
     CDS             complement(367..808)
                     /gene="tnpA"
                     /locus_tag="KLH46_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317271.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            862..>1087
                     /locus_tag="KLH46_004396"
     CDS             862..>1087
                     /locus_tag="KLH46_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0380769.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQSEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKE"
BASE COUNT          275 a          293 c          241 g          278 t
ORIGIN      
        1 aagaatatgg cgctcaacgg atacggcatt gcctggttac cgatctggtc gatcgtcgat
       61 gaactacaga ccaaacgtct gatatgcctt gatgctgcta aattgacagt ccccattcag
      121 gcttatattt accgaatgaa cacccgactg aatagaaccg ccgagaacct ctggcgtatt
      181 ttacaggaac atatgccaga cgatttaatc cagcagatat caatggaaga gccagcacgc
      241 cgcaattgac gacgtgcaat aaatatcctc cggcatagcc ggaggttttt caaatgcgcc
      301 tataaggctc tcttaccagc cgcgccctaa caggcgcaca cgatctgaca tttgcatcca
      361 acttcgttac ttacggtccg taaacgggct gcccggacag ggaatcgata actgctctcc
      421 cattttatcc tcttcaagct ggtgctttat gtaatcctgt atcttcgccg tgttcttacc
      481 cactgtatcg acgtagtccc ccctgcacca gaactccctg ttcctgtatt tgaatttcaa
      541 atcaccaaac tgctcgtaaa gcatcagact gcttttccct ttcagatatc ccataaagcc
      601 ggatacgctc attttgggcg ggatctccac aagcatatgg atatgatctg cacagcattc
      661 agcttccaga atccgtacgc ttttccactc acacagcttt ctcaaaatac tgcctgttgc
      721 tctacgcttc tctctgtaga acacctgtct tcggtatttt ggcgcaaaaa actatgtgat
      781 atttacagtt ccatcgggtg tgcgctatga accgccccgg gaatcctgga gactaaactt
      841 cctgagaaag aggtaaacag gatgactaaa aatactcgtt tttcccccga agtccgtcaa
      901 cgggcagtcc gtatggttct ggaaagtcag agcgaatatg actcacaatg ggcgacaatt
      961 tgttccattg ctccaaagat tggctgtacg ccggagactc tgcgtgtctg ggttcgccag
     1021 catgagcggg ataccggggg cggtgatgga gggctcacca ccgctgaacg tcagcgtctg
     1081 aaagagc
//
LOCUS       DAEEGA010000284         1068 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEEGA010000284 DAEEGA010000000
VERSION     DAEEGA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.289"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            18..560
                     /locus_tag="KLH46_004397"
     CDS             18..560
                     /locus_tag="KLH46_004397"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380770.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            787..>1068
                     /locus_tag="KLH46_004398"
     CDS             787..>1068
                     /locus_tag="KLH46_004398"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HBE0380771.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DAEEGA010000285         1066 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEEGA010000285 DAEEGA010000000
VERSION     DAEEGA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.290"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(570..749)
                     /locus_tag="KLH46_004399"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="KLH46_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="KLH46_004400"
     CDS             843..>1066
                     /locus_tag="KLH46_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0380772.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DAEEGA010000286         1051 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEEGA010000286 DAEEGA010000000
VERSION     DAEEGA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.291"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..217
                     /locus_tag="KLH46_004401"
     CDS             <1..217
                     /locus_tag="KLH46_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380773.1"
                     /translation="AEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRET
                     EQQIYRQLTDEVLALRLPENGSQLHHS"
     gene            complement(397..1020)
                     /locus_tag="KLH46_004402"
     CDS             complement(397..1020)
                     /locus_tag="KLH46_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBE0380774.1"
                     /translation="MLMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RTEQEAKPFTFEWNGRTWNADASSVARLSPVVMLAKSVAAQTHMVWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSSLDDLASIRAFDVK"
BASE COUNT          232 a          314 c          268 g          237 t
ORIGIN      
        1 ggcagaagag cagaaatatg agatgctgga gaatgagtac cctcagaggg tggctgaccg
       61 gctgaaagca tcaggtctga gcggtgatgc ggatgcggag agggaagccg gtgcacaggt
      121 gatgcgtgag actgaacagc agatttaccg tcagctgact gacgaggtac tggccctgcg
      181 attgcctgaa aacggctcac aactgcacca ttcataatca catcgcataa accacagacc
      241 ggactgactc cggaaaaaca gaggcccgcc cccgggcctc cccggattca tccgtttctc
      301 tgttcagcct gaccgcacac cccggcggcc ggatgacaga ctccgcctcg gtaagcaaag
      361 cggtcttctg tgattccgcc agttgcggct tattcattac ttaacgtcaa acgcccgaat
      421 tgaagccaaa tcatccagac tgctcagctc ctctttcatc tcccgctgac ggcgataaat
      481 ctcatcgttg cgatcgacct gcgcctgcac cattgctgcc gccagttctt ccagttccgg
      541 catcgacagt ttcacctgct gattatccgc atcactccac accatatgtg tttgtgccgc
      601 gacagatttt gccagcatga ccaccgggga caggcgggca actgacgagg cgtcagcgtt
      661 ccacgtgcgg ccgttccatt caaacgtgaa cggcttcgcc tcctgttctg tgcgccatgc
      721 ttcaatttcc tgacgtctgg cctctctggc cgcttccagc atttctggtg tcacagtgaa
      781 tggggctatc tcaccccatt tgccactttg cagttcctgc cagatttgct gacccgtcgg
      841 tgcaacatca tcagcggtgg ctgtgtaggg aactgcctgg tccctgtcgt caaaaaaaac
      901 gtcacagtct actgcgccac tttcggtata acggggatta atgatttttt taatttccac
      961 ggtgcattcc tcacgatgtg cgaataaaaa gcccgggcat tgcgccagag acatgagcat
     1021 ccggcacccc ggacagggcg caatatgacc c
//
LOCUS       DAEEGA010000287         1041 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEEGA010000287 DAEEGA010000000
VERSION     DAEEGA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.292"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..769
                     /locus_tag="KLH46_004403"
     CDS             <1..769
                     /locus_tag="KLH46_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE0380775.1"
                     /translation="QFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQRGYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQM
                     VFNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKPG"
     gene            790..>1041
                     /locus_tag="KLH46_004404"
     CDS             790..>1041
                     /locus_tag="KLH46_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBE0380776.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQS"
BASE COUNT          336 a          232 c          235 g          238 t
ORIGIN      
        1 tcaatttgca acagataatg gttatacatc aggaaatcca tttaacggtc tgacaccatt
       61 aaaaaagtcc aagatagaac cagatcccct cacccgtgac gaatttattc gttttattga
      121 ggcttgccgt catcaacaaa caaaaaacct gtggattatc gctgtataca cgggtattcg
      181 tcacggggag ctggtatcac tggcctggga agatatagac cttaaagcga ggactataac
      241 catccgccgg aattatacaa aactaggcga attcactcca ccaaaaactg atgctggtac
      301 cggaagaacg attcatctag ttcaaccagc tattgatgct cttaaaagcc aagcggaaat
      361 gaccatgctt ggaaaacagc attctgtaga ggtaaagcag aggggatatg ggagaagtac
      421 tgtgcataaa tgcacttttg tttttagtcc tcaggtaata aaacagcggc agttttccgg
      481 accgcactat aaggttgact ccatcaggga gtcatggaca agtatcttaa aacgcgcagg
      541 tctgagacac agaaaatcgt atcaatccag gcatacctat gcatgctggt cacttgccgc
      601 aggagctaat cctagtttta tcgcaagcca gatgggccac acaaacgcac aaatggtatt
      661 caatgtttac ggagcatgga tgaaagacaa caatcacgaa cagatagaac ttcttaacag
      721 aagactatct gaaagtgtcc catgcatgcc ccataagaaa ccggggtaaa ataaaaactt
      781 gcaaaatcaa ttggtttacc tttaatccct gtcacgttac gcgcgtggca gaggcgttac
      841 ggattgctga aaccgcaacg gacagacggc ggtcatcggc tgttcaacga tgccgatatt
      901 gaccggatcc gcgagatcaa acgctggatc gacaacggcg tgcaggtcag caaagttaaa
      961 atactgctca gtaatgaaaa tgttgatgtg cagaacgtct ggcgcgatca gcaagaaaca
     1021 ttactgactt acctgcaaag c
//
LOCUS       DAEEGA010000288         1028 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEEGA010000288 DAEEGA010000000
VERSION     DAEEGA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.293"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..>1028
                     /locus_tag="KLH46_004405"
     CDS             <1..>1028
                     /locus_tag="KLH46_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE0380777.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSA"
BASE COUNT          317 a          193 c          268 g          250 t
ORIGIN      
        1 tgtctataac cacgaaccgt tatgcatcag gttatgccac actgactgaa gcagtaagtg
       61 cacaggatga acggaacaga aaaagagata agaatagtca cgatggctct acgatatcct
      121 tatcccagcc ccttggtaat atcgggaatt tgaacttcaa taccacgagg tataattctt
      181 cccggggcac aggaaacacg cggtctacca gcctgagtta tagtacggta tggagaggca
      241 taacattctc cattaactgg gcaaaaaacg acttgctgac atcgcataaa tggaaagtag
      301 atcggaaatt aagcgtgggt atttcagtgc cattatcgct gggtgacgaa aatcaaattt
      361 atgcgtcttc acagatgagt cgttcgggag agcagggtaa taactatcag gtttcattat
      421 caggtcagaa tagtggtggt gtatggtggg atgtagcgac aaatataact aacgctcatc
      481 agtcacaacc gaagagcacg atgaacattg tgcaagttgg caaaaatgga tcatatggtc
      541 agtttagtag ccattacagc tccagcgaaa acatgaaaca gctaggtgca aatttaagcg
      601 gtgggatact tattacaagg gatggactta catttggtca aaatgtggat gggacattag
      661 cccttataga ggcacccgga gcaacggggg ttaacgttaa tggctggccg ggactgagta
      721 ctgattttcg tgggtatgca atactgccag tgcagccata ccgaagagac gatgttattc
      781 tggatgaaaa aacgataggt aagaattacg acttaccaca aactagtcaa ttggttgtac
      841 cgacagctgg agccgttgtg cctgcgacat tggcagtgaa gagcggggat aaaggattag
      901 taaccttaaa acagaaagag ggtaaacccg tccccttcgg cgctgtgatt agttatagca
      961 aagacactga aaatatggcc ggcattgtag gtgaagatgg tattgcttat gtttctggct
     1021 tgagtgcg
//
LOCUS       DAEEGA010000289         1021 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEEGA010000289 DAEEGA010000000
VERSION     DAEEGA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.294"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(50..190)
                     /locus_tag="KLH46_004406"
     CDS             complement(50..190)
                     /locus_tag="KLH46_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380778.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="KLH46_004407"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="KLH46_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBE0380779.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          296 a          206 c          237 g          282 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt ccccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgtta
     1021 t
//
LOCUS       DAEEGA010000290          983 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEEGA010000290 DAEEGA010000000
VERSION     DAEEGA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.295"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="KLH46_004408"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="KLH46_004408"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBE0380780.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          220 c          222 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAEEGA010000291          935 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEEGA010000291 DAEEGA010000000
VERSION     DAEEGA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.296"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..>935
                     /locus_tag="KLH46_004409"
     CDS             <1..>935
                     /locus_tag="KLH46_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBE0380781.1"
                     /translation="KKLDVSCNKLTILTDLPENIKELIARDNFLTHISALPHYLITLD
                     VSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPSLPSNLKI
                     LKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQITTIPTNL
                     FRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLRQMLPQNL
                     HSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRAFSAFLDR
                     LSDTVSARNTSGFREQVAAWLEKLSASAELRQQSF"
BASE COUNT          281 a          229 c          151 g          274 t
ORIGIN      
        1 ttaaaaagct tgatgtgagc tgtaataaat taaccatcct tactgattta cctgaaaata
       61 ttaaagaact tattgcaaga gataatttct taacacatat atctgcatta ccacattatc
      121 taataacttt ggatgtgtcc gaaaatcaat tagagaatct gccgttatta ccagacacca
      181 tcaaatcact aagcgcagag tataataggt tatccacact gccttcatta cccttgaatt
      241 taaaaaaact tgaggttagg aacaacgaac tgcaaactct tccatctctg ccttctaatc
      301 ttaagatact taaggttgcg cacaaccatc ttactgaact gcccccttta cctaggagac
      361 tgcaacttct ttttgcatat agcaatagat taagcaactt accaaacatc caagaaaata
      421 ttatcatgag aagatttttt tattttgaaa acaaccaaat aactacaatc ccgacaaatc
      481 tttttcgttt agatcctcat ataactattg agattgcaaa taacccctta tcagatcaaa
      541 ctctgctatt cttaatacag caaacttcgg ttccaaattt taacgggcct cagtttcgta
      601 tttccctgtc agaccaaaac agactgtttt tacgccagat gttgccgcaa aatttacatt
      661 cgcgccatat cagagtcatc actgaagggg ggcagaactt tcagatcccc cctcttcccg
      721 aaactgtggc agcctggttt cctgaagcag atcgtcggga ggtttctaca caatggactt
      781 ctttttccac cgaggagaat tcccgggcat tctccgcgtt ccttgaccgc ctttccgata
      841 ccgtctctgc acgcaatacc tccggattcc gtgaacaggt cgctgcatgg ctggaaaaac
      901 tcagtgcctc tgcggagctt cgacagcagt ctttc
//
LOCUS       DAEEGA010000292          933 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEEGA010000292 DAEEGA010000000
VERSION     DAEEGA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.297"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(55..243)
                     /locus_tag="KLH46_004410"
     CDS             complement(55..243)
                     /locus_tag="KLH46_004410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380782.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          245 a          181 c          217 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aag
//
LOCUS       DAEEGA010000293          927 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEEGA010000293 DAEEGA010000000
VERSION     DAEEGA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.298"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            1..927
                     /locus_tag="KLH46_004411"
                     /pseudo
     CDS             1..927
                     /locus_tag="KLH46_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT          214 a          234 c          301 g          178 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatgcgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
      541 ggtcagaggg gctggcgatg ccggagtcgt tgtcacatat cacgacggaa tcacagagga
      601 gaagcctggt gctgaaagcc ggaggaaaat actggcatgt gtacatgtcg aaaaaaacgg
      661 ccggcgggag gaatacggcg cgctacctgg gtcgttatct gaagaagccg ccgatagcgg
      721 cctcccggct ggcacattac aacggagggg gagcctgagc ttccgttacc tggaccacaa
      781 aacgggagaa acggcgacgg aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca
      841 gcacatcccg gagaagtttt ttaagatggt gaggtacttc gggttccttg ccaaccgggt
      901 gtgtggagag aagctgccgc aggtgta
//
LOCUS       DAEEGA010000294          863 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEEGA010000294 DAEEGA010000000
VERSION     DAEEGA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.299"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            1..525
                     /locus_tag="KLH46_004412"
                     /pseudo
     CDS             1..525
                     /locus_tag="KLH46_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            589..696
                     /locus_tag="KLH46_004413"
                     /pseudo
     CDS             589..696
                     /locus_tag="KLH46_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            761..>863
                     /locus_tag="KLH46_004414"
     CDS             761..>863
                     /locus_tag="KLH46_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0380783.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRL"
BASE COUNT          250 a          180 c          203 g          230 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg ttgcaacacg tctcgatatc accacctgag gtagcctgag tttaacggac
      721 actccttcct gaaatagaat ggcatcagaa ggagctaata atgagcagaa aaacccaacg
      781 ttactctaaa gagttcaaag ccgaagctgt cagaacggtt cttgaaaatc aactttcgat
      841 cagtgaaggc gcttcccgat tat
//
LOCUS       DAEEGA010000295          854 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEEGA010000295 DAEEGA010000000
VERSION     DAEEGA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.300"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..209
                     /locus_tag="KLH46_004415"
     CDS             <1..209
                     /locus_tag="KLH46_004415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380784.1"
                     /translation="DLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCMEREFATIPAG
                     INADGMYCPECGTVHWPDGVIPPF"
     gene            257..686
                     /locus_tag="KLH46_004416"
                     /pseudo
     CDS             257..686
                     /locus_tag="KLH46_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          212 a          170 c          268 g          204 t
ORIGIN      
        1 cagacctgaa tattccgttc tttgtggatg ccggaaacaa aaaactggtc ttctggtttg
       61 tacgtgttga tgacgaaggg tatccggaaa ttgcccgctg catggagcgg gaatttgcga
      121 caattcctgc cggtatcaac gccgacggca tgtattgccc tgaatgcggt acggttcact
      181 ggccggatgg cgttattccg cctttctgat gcttccccgt tttgccgata tttttcagca
      241 gggaaaccga tggcttaact ggctggagaa gcagccggtg cagatgagcc gcctggaaca
      301 ttatgccggt caggatgaaa ttggcctgcg ttacaacagc caccggacaa aacgagaaga
      361 aaaccttgtg atgagtggcg acgagtttat ggaaaggttc tcctggcatg tggcggataa
      421 ggggttccgg atggtgatac ggggtcctga gtccggtgaa gcggcgatta ctggaagatg
      481 tggtgtacgc cataacggag acagtgagaa aaacggcgag gcaaatcaca aggagaggga
      541 tgtatcagca gtgactgaag gttgacccgc tgaagtgcga gctgcgcgga ggtcagatgc
      601 ggtttacggg gctgaagagg ggttaccgtc tggcagagca ggttatgatg catgagccgc
      661 tggctcgaat acggtggtgc ggctgagagt cgcagagggg aagttgcgtc catgttgagg
      721 ggaacggagc aaaaaaccat cagagataca ttgaatcaat caatgtaagc aatattaagt
      781 gtctataccg attacttgat gatcaggttg tgttgaataa acgcgcattc ttcggtgctt
      841 ttttaatctt ttgg
//
LOCUS       DAEEGA010000296          833 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEEGA010000296 DAEEGA010000000
VERSION     DAEEGA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.301"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..326
                     /locus_tag="KLH46_004417"
     CDS             <1..326
                     /locus_tag="KLH46_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBE0380785.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="KLH46_004418"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="KLH46_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBE0380786.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          253 a          183 c          155 g          242 t
ORIGIN      
        1 ttagtgaaac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga gtg
//
LOCUS       DAEEGA010000297          820 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEEGA010000297 DAEEGA010000000
VERSION     DAEEGA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.302"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            52..820
                     /locus_tag="KLH46_004419"
                     /pseudo
     CDS             52..820
                     /locus_tag="KLH46_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          231 a          206 c          218 g          165 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt cgcaagaaaa ccagtgggct gggccatgtc
//
LOCUS       DAEEGA010000298          802 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEEGA010000298 DAEEGA010000000
VERSION     DAEEGA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.303"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            69..341
                     /locus_tag="KLH46_004420"
     CDS             69..341
                     /locus_tag="KLH46_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380787.1"
                     /translation="MVRKFDELEAKCAAQENKVIPVSTELPPANESVLLFDANGEGWL
                     IGWRSLWYTWGQKETGEWQWTFQVGDLENVNITHWAVMPKAPEAGA"
     gene            341..646
                     /locus_tag="KLH46_004421"
     CDS             341..646
                     /locus_tag="KLH46_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380788.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            646..>802
                     /locus_tag="KLH46_004422"
     CDS             646..>802
                     /locus_tag="KLH46_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0380789.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQAC"
BASE COUNT          260 a          178 c          192 g          172 t
ORIGIN      
        1 tcacagataa aacactctcc aggaaacccg gggcggttca gaaagtaaat gaaacagatg
       61 ctgaatacct ggtgcgtaaa ttcgatgagc ttgaagctaa atgtgcagca caggaaaaca
      121 aagtaatacc agtgtcaact gaactgccac cagcaaatga aagtgttttg ttattcgatg
      181 ctaacggaga aggctggcta attggctggc gttctctctg gtacacctgg ggacaaaaag
      241 aaaccggaga atggcagtgg acatttcagg tcggggacct tgaaaacgtc aatatcactc
      301 actgggcagt aatgccaaaa gcaccggagg ctggagcata atgaccactt ttaccgacaa
      361 agaactgatt aaagaaatta aagagcgtat cagcagcctt gacgtgcgag acgatattga
      421 gcgccgtgct tatgaaatcg cactcctatc tctggaagta gaaccagatg aacgcgaagc
      481 ttatgaatta ttcatggaaa agcgttttgg tgacttagta gatcgtcgga gagcaaaaaa
      541 cggcgataac gaatacatgg catgggatat gactctcggt tggatcgtct ggcagcaacg
      601 agctggtatc catttctcaa caatgtcaca gcaagaggtg aaataatgga gccatacagc
      661 ctcacactcg atgaggcctg tcattttctc aagatatcca gaccgactgc cattaactgg
      721 atacgcacag ggcgtcttca ggcaacacgc aaagatccca ctaagaataa atctccttac
      781 ctcacaacac gacaagcctg ca
//
LOCUS       DAEEGA010000299          764 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEEGA010000299 DAEEGA010000000
VERSION     DAEEGA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.304"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            68..>764
                     /locus_tag="KLH46_004423"
     CDS             68..>764
                     /locus_tag="KLH46_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0380790.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRR"
BASE COUNT          261 a          163 c          189 g          151 t
ORIGIN      
        1 cggctcttca gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg
       61 aaagaaaatg tcactcttcc gcagaggtga aatatggtac gccagtttca cattgccgaa
      121 cggtaaaaga tttaaacagt ctcttggaac aaaggacaaa aggcaggcga cagaactcca
      181 tgacaagcta aaggctgaag catggcgggt cagcaaactt ggtgaaatac ctgatataac
      241 gttcgaggaa gcgtgtgtca ggtggcttga agagaaagca cataaaaaat cactggacga
      301 tgacaaaagc cggatcggat tctggcttca acatttcgca ggaatacaac taagagacat
      361 tactgaatca aaaatttatt cagcaatgca gaaaatgacg aaccggcgtc atgaggaaaa
      421 ctggaaactc agggcagaat catgcagaaa aaaagggaaa cctgttccag aatacacgcc
      481 aaaaccagcg tccgttgcaa cgaaggctac gcatctttca tttataaagg ccctactaag
      541 agccgcagag cgtgaatgga aaatgctgga taaggcacca attattaaag tgcctcaacc
      601 aaagaataaa cggatccgct ggctggagcc ccatgaagca caaaggctga ttgatgaatg
      661 tccggagcca ttaaagtctg ttgttgaatt tgcactggca acaggcttaa gacgctcgaa
      721 catcatcaac cttgaatggc aacaaataga tatgcagcgc cggg
//
LOCUS       DAEEGA010000300          758 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEEGA010000300 DAEEGA010000000
VERSION     DAEEGA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.305"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            202..>758
                     /locus_tag="KLH46_004424"
     CDS             202..>758
                     /locus_tag="KLH46_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBE0380791.1"
                     /translation="MIKSTNIQVIGSGIMHQINKIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          264 a          145 c          140 g          209 t
ORIGIN      
        1 acatcacccc ccggatcatg gacgactggg ggcagtaatc atgccgggtc tggctggcta
       61 acaccccatt ataaggggta cgaataaagt aacgggggaa gcaatacctg gagaaagagt
      121 acaaagcaaa ctgtattaaa cttaataagt tatattgtat ttcactagag ttcttatcgt
      181 tgataagaaa ttctggttat aatgattaaa tcaacgaata tacaggtaat cggttctggt
      241 attatgcatc aaataaacaa aatacactcg ttaactctat tttctttgcc tgtgagtttg
      301 agtccatctt gtaatgaata ttatttaaaa gtctggagtg aatgggaaaa gaacggtacc
      361 ccaggcgagc aacgcaatat cgccttcaat aggctaaaaa tatgtttgca aaatcaaggg
      421 gcggaattaa atctatctga gttagattta aaaacattac ctgatttacc gcctcagata
      481 acaacactgg aaataagaaa aaacctatta acatatctcc ctgatttccc accaatgctt
      541 aaggtaatac atgctcaatt taatcaactg gaaagcttac ctgccttacc cgagacgtta
      601 gaagagctta atgtgggtga taacaagata aaagaattac catatcttcc tgaaactcta
      661 actcatttac ggatccataa taaccgattg catattctgc cactattgcc accggagcta
      721 aaattactga tagtttctgg aaacagatta gacagcat
//
LOCUS       DAEEGA010000301          756 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEEGA010000301 DAEEGA010000000
VERSION     DAEEGA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.306"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..>756
                     /locus_tag="KLH46_004425"
     CDS             <1..>756
                     /locus_tag="KLH46_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBE0380792.1"
                     /translation="RILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFELD
                     LEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGTD
                     LFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDG
                     FSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDV
                     VNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          210 a          155 c          182 g          209 t
ORIGIN      
        1 cgcattctga ccgaccgtga aaatggtgaa aaaggcgata attctcaaga tacctcttat
       61 gcccgtgtag gcgtgaaagg tgaaactcag atcaaccctg aaatgactgg ttacggtcag
      121 tttgaactag acctggaagc atcaaacaga cacaaccctg accaaactcg tttggcatat
      181 gctggtttga gttacaaaga ttttggttca ttcgactaca gtcgtaatgt cggtgtcgcg
      241 tatgatgcag aagcatttac tgatatgttt gttgagtggg gcggtgactc atgggctggt
      301 actgacctgt tcatgaccaa ccgtaccaac ggtgttgcaa cctatcgtaa cacagatttc
      361 tttggcatgg ttgaaggtct aaattttgct ctgcaatatc agggtaaaaa tgaaggtaca
      421 ggcaactaca aagcaaatgg cgatggccat ggtttatctg caacttacac tattgacggt
      481 ttctcatttg ctggtgctta tgcaaactct gaccgtactg attggcagtc tggtgatggt
      541 aaaggcgaaa gagccgaagt ttgggcgttg tcaacaaaat atgacgctaa caatgtttat
      601 gctgctgtga tgtacggtga atctcacaac atgaattccg acgacggtga tgttgtgaac
      661 aaaacacaaa acttcgaagc tgttctgcaa tatcagttcg actttggtct gcgtccatcc
      721 atcggttact cctattctaa agcactggac gtagca
//
LOCUS       DAEEGA010000302          752 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEEGA010000302 DAEEGA010000000
VERSION     DAEEGA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.307"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..707)
                     /locus_tag="KLH46_004426"
     CDS             complement(<1..707)
                     /locus_tag="KLH46_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HBE0380793.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNGWSLAGEHRH"
BASE COUNT          168 a          197 c          223 g          164 t
ORIGIN      
        1 cagtggcggt gttctcccgc caggctccag ccatttaaca gtcccagtga gtaataacct
       61 ttatccatta acgtcagagt gttatcgccg gtttgttcta taagttgctc agcaagctca
      121 ttttcgctgt tcttcatcgt gccgaaggct gcagccgtca gcagatggct ggtcagttcc
      181 atctggcaga ccattttgac ctgcgggtag agcgccgggt tcccggcatg tgtctggcgg
      241 gggaaggctg catcgttctc tggtgtatcc ggtgtgcgcc agaacacacc atcgatggcc
      301 agcagggtca ggccgcacca gtgcggatgc ggcgtggcgt tatgccagag ctgcgctgtt
      361 ttcgtgaaca cgcggcggac agcctcactt cccaggcgct ggcgggcctg aataacggca
      421 ctgggggcaa cgaaggggcg attgcccggc agcatgatgt ccaggcgatt cacaatctgg
      481 tgaagaggtt ctttacgctc aagcgccatg ccaacaatac accagaccat catttcgagg
      541 ggaagacggc gcttgcgtag cgttacagta cctgattcgg caaggcaacg agagatgagt
      601 tcggggtcga ggtaatcccc cagagaagtc agtgggttac gcagagaatc gtaacgggat
      661 accagatcaa gagcctgtcc aatgtgcata aaaaaatccg gaaacaagtg agcgtttccg
      721 gattcttaca cagccactgg atcggtcaac tg
//
LOCUS       DAEEGA010000303          742 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEEGA010000303 DAEEGA010000000
VERSION     DAEEGA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.308"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            19..492
                     /locus_tag="KLH46_004427"
     CDS             19..492
                     /locus_tag="KLH46_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0380794.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          234 a          148 c          152 g          208 t
ORIGIN      
        1 ggagatgaac cctatgcaat gtcctggtta atatctcagt gtgctcatca gtgcactgat
       61 aataaaaaga cggagacaga tgccatttac gataaagttc ggagttctta tttgctttca
      121 tgtattctga aaaaaaacaa aaatgttggg cttattcttc atgcaccatc atttgtttct
      181 gtgtctgaaa aaatcgccag aattgtgatg gcaaattact cccgtaactg gagtaattcc
      241 gagttggcca gtgcggtttt aatgagtgaa tcttctttaa aaagaagaat gtacaaagag
      301 gtcggttcta taagtacatt tgtacataaa ataaagctta cagaagctat caggaaatta
      361 cgaaggacaa atactcccat ttctgtcata tcatctgagt taggttacag ttcaccatcg
      421 tatttttcta aagtcttctt taaatattta aagacatacc ctcaaaacat aaggaagaaa
      481 aatggtcggt aatgtcacca attaggagga cttgcagagc cgctttttta cgatactgag
      541 atcaaacaga cataatctat gcggagattt acagcagggc atggttcaca aatctgacag
      601 cgatgaatta tcggcactgc gggcagaaaa tgctcgaatc atcaagcccc tgttacctcc
      661 tgaacccgcc acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg
      721 catgttctgg gtgttatgtt cc
//
LOCUS       DAEEGA010000304          692 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEEGA010000304 DAEEGA010000000
VERSION     DAEEGA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.309"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            36..>692
                     /gene="istA"
                     /locus_tag="KLH46_004428"
     CDS             36..>692
                     /gene="istA"
                     /locus_tag="KLH46_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBE0380795.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     P"
BASE COUNT          160 a          177 c          182 g          173 t
ORIGIN      
        1 agggggtaat ctgctctcct gattcaggag agcttatggt cacttttgag acagttatgg
       61 aaattaaaat cctgcacaag cagggaatga gtagccggac gattgccaga gaactggggc
      121 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
      181 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      241 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      301 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      361 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      421 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      481 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      541 gtaatgcgtt ccgcttcttt ggtggtgtgc cgcgcgaagt gttgtatgac aatatgaaaa
      601 ctgtggttct gcaacgtgac gcatatcaga ccggtcagca ccggttccat ccttcgctgt
      661 ggcagttcgg caaggagatg ggcttctctc cc
//
LOCUS       DAEEGA010000305          687 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEEGA010000305 DAEEGA010000000
VERSION     DAEEGA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.310"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..665)
                     /locus_tag="KLH46_004429"
     CDS             complement(<1..665)
                     /locus_tag="KLH46_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBE0380796.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          185 a          175 c          154 g          173 t
ORIGIN      
        1 gccgcatcat catcagactc cggaacgccc tcatacacat gtttaaaacc atccggatca
       61 cggcgtcgcg ccgcgtcgat aaccttaagc atggtgtcag acagaaacgg attttcgtca
      121 tagttaattt tgcggatgag agtgccttcg ggcggatcaa caacgaagtt gcgccagacg
      181 aaatcagtaa caagtccggg gttgaatatg aaccagcatt ccgaaccctc tttacggatc
      241 gttggctcca gaattttcca ctggtattcc gtcagtgcgt gggcttcttc cagccacaac
      301 atatcgatcc cctccagtga cttaatttct tcgatgttgc gccataatcc ataaaaaaca
      361 aattccgagc cagtaacccg gttaatgatt ttgttgttca gaatacggaa acggtgccgc
      421 agaccaaacc tgtctatctg aattttgagc agggtataca ccgactcttc gattttattc
      481 tggatctgac gggcacaaca aaaacgcagg gtgtatttat tcgacagaaa tatggcaatg
      541 ccagcggcat cccatgattt tgacgatgac cgaccaccat aaagcacttt gttacgtgcc
      601 cgcatagtcc agaaactacg taaagccgga ttcagcgtcg gtttggatgt cagagtagaa
      661 gtcattgagg tcacgctctc cattgcc
//
LOCUS       DAEEGA010000306          681 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEEGA010000306 DAEEGA010000000
VERSION     DAEEGA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.311"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..>681
                     /locus_tag="KLH46_004430"
     CDS             <1..>681
                     /locus_tag="KLH46_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBE0380797.1"
                     /translation="TLADNFKQKNEALDTWYVGMNDFARIAGGQNSRSNILSPRAFLE
                     FLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKFYMNNPEGERADLS
                     KVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDDSSLHLITNKVLAC
                     YQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNNSQVSCKGSVDSTI
                     TDLLEKALN"
BASE COUNT          238 a           99 c          147 g          197 t
ORIGIN      
        1 acattagccg acaattttaa acaaaaaaat gaagccttag atacctggta tgtgggaatg
       61 aatgattttg cccgaattgc cggggggcag aatagcagaa gcaatattct ttctcctaga
      121 gcatttttgg agtttttggc aaagatattt accctgggtt atgtggattt tagcaaacgc
      181 tccaacgaag cgggtagaaa tatgatggct catattgagt tctcatctta ttctaaagat
      241 actgatggca atgaaaaaat gaagttttac atgaataatc ctgaagggga gcgagcggat
      301 ttatctaagg tgaagataga aattacactt gcctcagctt ctactaaagg gattcgtgaa
      361 ggacatacag tcattatatt taaacaatct gatggttcga ctaatcgtta tgaagggaag
      421 tcttttgaaa gaaaagatga tagttcatta cacctgatta ctaacaaggt tctggcgtgt
      481 taccaacgcg aagctaacaa gaaaatagcg cgtctattaa ataatcatca gaagttaaat
      541 aatatacagg agttaaatga ttcgcaggag ttaaataatt cacagaagtt aaataattcg
      601 caggagttaa ataactcgca ggtgagttgt aaaggttcag ttgattctac gattacggat
      661 ttattagaaa aagcattgaa t
//
LOCUS       DAEEGA010000307          656 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEEGA010000307 DAEEGA010000000
VERSION     DAEEGA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.312"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..492
                     /locus_tag="KLH46_004431"
     CDS             <1..492
                     /locus_tag="KLH46_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0380798.1"
                     /translation="VLGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAF
                     LKIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLA
                     DRFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMF
                     TEM"
     gene            554..>656
                     /locus_tag="KLH46_004432"
     CDS             554..>656
                     /locus_tag="KLH46_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0380799.1"
                     /translation="MSRKTRRYSKEFKAEAVRTVLENQLSISEDASRL"
BASE COUNT          166 a          142 c          162 g          186 t
ORIGIN      
        1 gtactgggag cattgtgggt gcgcaatggc atggaggagt ccgcggaatt tgaacaacag
       61 caacataatc aagcggccgc gaaaaaacgc atcccggtta tcgaagctct gttacgacat
      121 cccggtgctt tcctgaagat tattgcacta cgactgtgcg agttgctgac gatgtacatt
      181 gttactgcct ttgcacttaa ttattcaacc cagaatatgg ggttaccgcg cgaacttttc
      241 cttaatattg gtttgctggt aggtggatta agctgcctga caattccctg ttttgcctgg
      301 cttgccgatc gttttggtcg gcgcagggtt tatatcacag gcgcgttgat cggaacgttg
      361 agcgcatttc ctttctttat ggcgcttgaa gcacaatcta ttttctggat agttttcttc
      421 tccataatgc tggcaaacat tgcgcatgac atggtggtgt gtgtgcaaca accgatgttt
      481 accgaaatgt gaggtagcct gagtttaacg gacactcctt cctgaaatag aatggcatca
      541 gaaggagcta ataatgagca gaaaaacccg acgttactct aaagagttca aagccgaagc
      601 tgtcagaacg gttcttgaaa atcaactttc gatcagtgaa gacgcttccc gattat
//
LOCUS       DAEEGA010000308          636 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEEGA010000308 DAEEGA010000000
VERSION     DAEEGA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.313"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            88..153
                     /locus_tag="KLH46_004433"
                     /pseudo
     CDS             88..153
                     /locus_tag="KLH46_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="KLH46_004434"
     CDS             381..629
                     /locus_tag="KLH46_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBE0380800.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DAEEGA010000309          630 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEEGA010000309 DAEEGA010000000
VERSION     DAEEGA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.315"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..112)
                     /locus_tag="KLH46_004435"
     CDS             complement(<1..112)
                     /locus_tag="KLH46_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBE0380801.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYI"
     gene            complement(109..399)
                     /locus_tag="KLH46_004436"
     CDS             complement(109..399)
                     /locus_tag="KLH46_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBE0380802.1"
                     /translation="MVDLLKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(399..>630)
                     /locus_tag="KLH46_004437"
     CDS             complement(399..>630)
                     /locus_tag="KLH46_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBE0380803.1"
                     /translation="FREWVAIQAGFYTEHFYPDGSRGCRAKSIAFANMDETEFQQVYK
                     SVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
BASE COUNT          149 a          181 c          136 g          164 t
ORIGIN      
        1 caatatacga ccagcgcccc cacatacgta gttttccctg aatccagaca ctttccagcg
       61 tggtgagacg aaggtgttct ccgctttttc ctgtattcgt tgggtaaatc ataaatatcc
      121 ctcctttctc cagatttctt gtgtgcgaaa aacaccttct gcatgcatca ggcgtaattc
      181 ttctttggtg taatcgctgg tttttacccg cccgtcgatt aaatcgtggc acgagctaca
      241 ggcaatcgct gcctgcatat cgtgtggctt tatcgctgtt ccgcacgttc ccgccagtcg
      301 gtaatgcgcc agcacagacg tttccggatc gtgattgcag tagccaggaa ttctgacggt
      361 gcacatctgc ccccgcgccg ctttaagtaa atccaccatt acgcaaactc cagcagctgc
      421 gcggccacat tttcgacttg ttccggagag gaaaatttac ggaacagaat ccagttccac
      481 agcacattca gtacagattt ataaacctgc tgaaactcgg tttcgtccat attcgcaaac
      541 gcgatagatt tcgcccgaca cccacggcta ccgtccggat aaaaatgctc ggtgtaaaat
      601 ccggcctgaa tggctaccca ctcgcggaaa
//
LOCUS       DAEEGA010000310          628 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEEGA010000310 DAEEGA010000000
VERSION     DAEEGA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.316"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..131)
                     /gene="csgC"
                     /locus_tag="KLH46_004438"
     CDS             complement(<1..131)
                     /gene="csgC"
                     /locus_tag="KLH46_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBE0380804.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQI"
     gene            complement(190..555)
                     /gene="csgA"
                     /locus_tag="KLH46_004439"
     CDS             complement(190..555)
                     /gene="csgA"
                     /locus_tag="KLH46_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBE0380805.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT          164 a          142 c          150 g          172 t
ORIGIN      
        1 aatatttgta ctctgcacag acaagattga gtaagagtaa cttcaggaat aatggtgtac
       61 atatcccctt gctgggtcgt attaaaggtt atctggctgg aaagtgccgc aaggagtaat
      121 aacgcattca tattcttctc ccgaaaaaaa acagggcttg cgccctgttt ctgtaataca
      181 aatgatgtat taatactgat gagcggtcgc gttgttacca aagccaacct gagtcacgtt
      241 gacggaggag ttcgatgcag tctggtcaac tgcagcaccg ttgccaccac cgaactgttt
      301 aacagtcatt tcagaatttt tgccgttcca ctgatcaaga gtggcgctgt taccgaagcc
      361 acgttgggtc agatcgattg agctgtcatc tgagccctga ccaacatctg caccattacc
      421 accgccatgc tgggtaatag tcaagtcaga gttacgggca tcagtttgca gagcaagtgc
      481 agagttaccg ccaccgtact ggtaaatgtt cagctctgaa tttgggccgc tattattact
      541 gaggtgtact ggcaatagcg gacactacca tttgttcttt tttaagcagc catctgatga
      601 tatttttccc tgaaggctgc cggggaga
//
LOCUS       DAEEGA010000311          601 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEEGA010000311 DAEEGA010000000
VERSION     DAEEGA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.317"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..127
                     /locus_tag="KLH46_004440"
     CDS             <1..127
                     /locus_tag="KLH46_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HBE0380806.1"
                     /translation="VTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            complement(161..364)
                     /locus_tag="KLH46_004441"
                     /pseudo
     CDS             complement(161..364)
                     /locus_tag="KLH46_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            464..>601
                     /locus_tag="KLH46_004442"
     CDS             464..>601
                     /locus_tag="KLH46_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0380807.1"
                     /translation="MRMNMSDFATSFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LK"
BASE COUNT          156 a          146 c          137 g          162 t
ORIGIN      
        1 ggtcacctgc aaacagaacg aagtggagcc ggaggactgg ttgcgcgaag tgatcgagaa
       61 gctcaatgac tggccgtcga accaagtgca tgaactgctg ccctggaact tctcgtctgt
      121 aaaataatcc ttacgctacg tacttctcgg ggcgcttacc tggagaataa cgtttatcca
      181 tattcttcca gtctggttca tcttccgacg tcatcaccgg gttgaaatta cgccgatata
      241 aatcgagttc cgtcacctgt attttccgct ccattgcccg ctgatggatc gcatcagcaa
      301 tatttgcggt caatgaatca tgacgaggat gggcccatac caaataaata cgttcagact
      361 gcataatttt acctccacgt taagttctgg agcgcacttt acgcaggcgg cataatcctg
      421 taaacgtgat gattcaggat gagttaatga aggattttca ctaatgcgga tgaatatgtc
      481 tgactttgcc acttcctttg ccgtggcccg taatcaaagc tttcgtgcag caggcgatga
      541 gttaggctta tcctcgtccg ccattagcca tagtattaaa acactggaac aacgtcttaa
      601 a
//
LOCUS       DAEEGA010000312          566 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEEGA010000312 DAEEGA010000000
VERSION     DAEEGA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.321"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..297
                     /locus_tag="KLH46_004443"
     CDS             <1..297
                     /locus_tag="KLH46_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBE0380808.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="KLH46_004444"
     CDS             complement(405..>566)
                     /locus_tag="KLH46_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380809.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAEEGA010000313          529 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEEGA010000313 DAEEGA010000000
VERSION     DAEEGA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.325"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            1..238
                     /locus_tag="KLH46_004445"
                     /pseudo
     CDS             1..238
                     /locus_tag="KLH46_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            338..>529
                     /locus_tag="KLH46_004446"
     CDS             338..>529
                     /locus_tag="KLH46_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380810.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDL"
BASE COUNT          181 a          109 c          104 g          135 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcatat tggctacgta attaaaacag
       61 aaaataaaga aaccccattc actgtagttg atatcgacgg tctatcagga aactttaaaa
      121 ctcttgttga aggtgccaca aaaatgtgcc tagtttacat cggaaataat ctgcccgcag
      181 aaaaaaagcc gaatttctgg caaatctgat tgcaatgaaa ttaaaaggtg aaatctgaaa
      241 aaaagaaagc ctgcacagtg tgcaggcctg agtgaagaac ctgggacatt tattcatcac
      301 tcgcagtaat tttaatctga gttgaggtta aaaaacaatg agcacaaaac cactcttcct
      361 gttacggaaa gcgaaaaaat catccggtga acctgacgtc gtcctgtggg caagcgacga
      421 ttttgaatcg accagcacca ctctggacta tctgctcgtt aagtcaggta aaaaactgag
      481 caactatttt aaagctgttg ccacaaattt tcctgtcgtt aatgacctt
//
LOCUS       DAEEGA010000314          526 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEEGA010000314 DAEEGA010000000
VERSION     DAEEGA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.326"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..>526)
                     /locus_tag="KLH46_004447"
     CDS             complement(<1..>526)
                     /locus_tag="KLH46_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0380811.1"
                     /translation="AVRLRYAFIRDNSCCWPVRLLCRVLDVHPSGFYAWLQQPHSQRH
                     QADLRLTGQIKQFWLESGCVYGYRKIHLDLRDSGQQCGVNRVWRLMKRVGIKAQVGYR
                     SPRARKGEASIVSPNRLQRQFNPDAPDERWVTDITYIRTHEGWLYLAVVVDLFSRKII
                     GWSMQSRMTKDIVLN"
BASE COUNT          121 a          146 c          136 g          123 t
ORIGIN      
        1 gttcaggaca atgtcctttg tcatccggga ttgcatggac cagccgataa ttttgcgtga
       61 gaacagatca acaacaacgg caagatacag ccagccttcg tgggtcctga tgtaggttat
      121 gtccgttacc caacgctcat caggagcatc cggattgaac tgtcgctgga gcctgttggg
      181 tgacacgata ctggcctcgc ctttacgtgc ccgcgggctt cggtatccga cctgagcctt
      241 tattccgaca cgtttcatca gtctccagac tctgtttact ccgcactgtt gcccgctgtc
      301 acgcagatcc agatggattt tgcgataacc atagacgcat cccgattcca gccagaactg
      361 tttaatctgt cctgtcagtc tcaggtctgc ctgatggcgt tgtgaatgcg gctgctgaag
      421 ccaggcgtaa aaaccactgg gatgaacatc cagcacccga cagagcaggc gaacaggcca
      481 gcaacaggag ttgtcacgga taaaggcgta cctcagtcgg acagct
//
LOCUS       DAEEGA010000315          516 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEEGA010000315 DAEEGA010000000
VERSION     DAEEGA010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.327"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            3..170
                     /gene="xisR"
                     /locus_tag="KLH46_004448"
     CDS             3..170
                     /gene="xisR"
                     /locus_tag="KLH46_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014884030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HBE0380812.1"
                     /translation="MLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWYN
                     YPKINQFIKDS"
     gene            172..342
                     /locus_tag="KLH46_004449"
                     /pseudo
     CDS             172..342
                     /locus_tag="KLH46_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            complement(359..>516)
                     /locus_tag="KLH46_004450"
     CDS             complement(359..>516)
                     /locus_tag="KLH46_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0380813.1"
                     /translation="ISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSVELHDKVIGHY
                     LNIKHYQ"
BASE COUNT          150 a          103 c          123 g          140 t
ORIGIN      
        1 tgatgcttcg cactggtctg ggtgccagac aaattgaagc ataccgccag aactgttggg
       61 tggaaggctt ccacttcaaa cgagtatctc ctttagggaa gccagacagt aagcgaggga
      121 ttatctggta caactatccg aagataaatc agtttatcaa agactcatga tatgtctaaa
      181 ttaccaacag gtgtcgagat tcgaggtaaa tacattcgca tctggttcat gtttcgagga
      241 aaacgatgtc gggaaacatt gaaaggctgg gagattacta acagtaacat taaaaaggcc
      301 gggaatttaa gagcgttgat agttcatcaa atcagttccg gtgggtaatg actccaactt
      361 actgatagtg ttttatgttc agataatgcc cgatgacctt gtcatgcagc tccaccgatt
      421 ttgagaacga cagtgacttc cgtaccagcc ttgccagatg ttgtctcaga ttcagattat
      481 gtcgctcaat gcgctgagtg taacgcttgc tgataa
//
LOCUS       DAEEGA010000316          484 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.328, whole genome shotgun sequence.
ACCESSION   DAEEGA010000316 DAEEGA010000000
VERSION     DAEEGA010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.328"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..>484)
                     /locus_tag="KLH46_004451"
     rRNA            complement(<1..>484)
                     /locus_tag="KLH46_004451"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          101 a          144 c          118 g          121 t
ORIGIN      
        1 ggtgtgtaca aggcccggga acgtattcac cgtggcattc tgatccacga ttactagcga
       61 ttccgacttc atggagtcga gttgcagact ccaatccgga ctacgacgca ctttatgagg
      121 tccgcttgct ctcgcgaggt cgcttctctt tgtatgcgcc attgtagcac gtgtgtagcc
      181 ctggtcgtaa gggccatgat gacttgacgt catccccacc ttcctccagt ttatcactgg
      241 cagtctcctt tgagttcccg gccggaccgc tggcaacaaa ggataagggt tgcgctcgtt
      301 gcgggactta acccaacatt tcacaacacg agctgacgac agccatgcag cacctgtctc
      361 acggttcccg aaggcacatt ctcatctctg aaaacttccg tggatgtcaa gaccaggtaa
      421 ggttcttcgc gttgcatcga attaaaccac atgctccacc gcttgtgcgg gcccccgtca
      481 attc
//
LOCUS       DAEEGA010000317          480 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEEGA010000317 DAEEGA010000000
VERSION     DAEEGA010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.329"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(52..480)
                     /locus_tag="KLH46_004452"
                     /pseudo
     CDS             complement(52..480)
                     /locus_tag="KLH46_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="The RecE (also known as exonuclease VIII) pathway
                     is an alternative pathway for the initiation of homologous
                     recombination in Escherichia coli. The RecE pathway is
                     activated in recB, recC sbcA (suppressors of recB and
                     recC) mutants. Activation of the RecE pathway results in
                     the production of at least two phage proteins relevant for
                     recombinational repair. RecE is a duplex DNA-specific
                     exonuclease which produces 3' overhangs in duplex DNA by
                     preferentially degrading the 5'-ending strands.
                     Recombinational repair of E.coli chromosomal DNA involves
                     as well other proteins: RecA, RecF, RecJ, RecO, RecR, and
                     RuvC.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
BASE COUNT           99 a          122 c          124 g          135 t
ORIGIN      
        1 gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct cagccttaat
       61 tgcatcagtc aggcggagca gggcggcatt atccagagtt gtcggttctg ccatgccgcg
      121 tatggccaac agcagattct ggacatagct gttttcctga tccatctcaa gagcagtaat
      181 gtgtttgcgt tgttcacggg tggcatgatg caggtatttc cgatccccgg cagcatacgt
      241 aaaaatgtgc agaagacgct gtgtgaaccg caaagtggct acagagactt cgcaatcctg
      301 gcaatcctcg tgggcgtctg cctgcgcgtt ttcttcctgg cctcccgcca gttctttggt
      361 ttcttgagca ttatcctggt ggtgaacgtc gtctggcgct gctcccggtt ttagttccca
      421 tgtcatggag tctttgctga gttgatagcg ttcactccag gtaaaatcga tctcaccttc
//
LOCUS       DAEEGA010000318          454 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEEGA010000318 DAEEGA010000000
VERSION     DAEEGA010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.331"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            60..454
                     /locus_tag="KLH46_004453"
                     /pseudo
     CDS             60..454
                     /locus_tag="KLH46_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          100 a          117 c          120 g          117 t
ORIGIN      
        1 acgaatcgga aaatcgatac tggaaaaact ctaactcggt ggccagtttt tgttgaccac
       61 ttcacgctcg catggtcgac gttctggaat cctctaagct gctgaagacc gtagagcttc
      121 ctgtcctgac tggtagcctg accgctaagg cagacgctat catggacgct ctgtatgaga
      181 acaccgagcc ttctttcggt tctgaggtgt ctgactacgg tattatcgcc catgagagcc
      241 aactgaaagc actgtcccgt ctggctgcta aacagggctt ctctggtgaa gacgctatcg
      301 tggatatgct gggtaccgac atcgcgtatt acaacggtga ggacaaaggt gtgttcatgt
      361 tggctaaacg cttcacggcc ctgagcttcg gctgcttccg tcacgacggt gagaatatta
      421 cggtcgtcct ttcacgtgat ggcgactctc agtc
//
LOCUS       DAEEGA010000319          446 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEEGA010000319 DAEEGA010000000
VERSION     DAEEGA010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.332"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..>446)
                     /locus_tag="KLH46_004454"
     CDS             complement(<1..>446)
                     /locus_tag="KLH46_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HBE0380814.1"
                     /translation="ILSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLL
                     MFNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVR
                     EKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEH"
BASE COUNT          102 a          113 c           86 g          145 t
ORIGIN      
        1 catgttcgaa caccactcca acatctgttt gaacacctgt taaatttctc tcttgttttg
       61 tttgaacatc tgcttccttt ctgcttcttc tggcctgaac agatgctttt cctgcggctg
      121 attttttggt taatttttct ctgacagatg ccagatcttc ctcaatccga agatgcatcc
      181 attcgtcgcc gttatcacaa aaaaactccc gcaaggatgg ttcaacatca acccatcgct
      241 cgttagtcag acgggcaatt tttgccaacc tgtttttggg tattggcttt cctgtttgcc
      301 agtaattgaa catcagcaac aaatacgcgc cgtgctcctc tgctgacaaa tgcatggtgt
      361 cagccaggta atcagctatg tacagttgca tgtatggtaa tgcggccata attgccccgt
      421 atgatgctac ccggttgctt agaata
//
LOCUS       DAEEGA010000320          446 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.333, whole genome shotgun sequence.
ACCESSION   DAEEGA010000320 DAEEGA010000000
VERSION     DAEEGA010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.333"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..395)
                     /locus_tag="KLH46_004455"
     CDS             complement(<1..395)
                     /locus_tag="KLH46_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380815.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGT"
BASE COUNT          105 a          123 c          123 g           95 t
ORIGIN      
        1 gtaccgctta ttgcgtgacc gatgacgtca atcatccatg ctgacaggtt ttgatgagca
       61 agttgctcga gtgattcgga tgtctggtca cgcagctggt tgtcgcagtg ccagcacaat
      121 accattgcgc cggtaccata acggtgaatg acggtttcgc tgtgatgata atcgccgtgt
      181 ggccactggc aggatttaat atggcgcaac agccagtcag acaatgcgcc agcaccacca
      241 gcagcacgaa tcacccgtgc gttactgaaa aacggcagca atgttttgtc ttccactagc
      301 ggctggcgaa cggcaggaac gaccccggac ggcagattac gcatgctttt cggttccggc
      361 tccaccagta cccgggtatt gtggaatacc ggcatggatt cacgaccggg cttaaggacc
      421 accagcccga gttccggaac cagaac
//
LOCUS       DAEEGA010000321          444 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.334, whole genome shotgun sequence.
ACCESSION   DAEEGA010000321 DAEEGA010000000
VERSION     DAEEGA010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.334"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..373)
                     /locus_tag="KLH46_004456"
     CDS             complement(<1..373)
                     /locus_tag="KLH46_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0380816.1"
                     /translation="MVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFGGM
                     VRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEFDI
                     WNLNETWAFQEKLIFCEDESSL"
BASE COUNT          134 a          106 c           95 g          109 t
ORIGIN      
        1 acagacttga ttcatcctca caaaatataa gtttttcctg gaatgcccat gtctcattga
       61 ggttccagat atcaaattcg aagtcgtgat aacgaaaacg gatcccacca aacttattaa
      121 aacgcagttg tttgacgggc agttcagcca atgtctggaa gagttcctca cgggagcgac
      181 cgatgacgat atcaaggtct gaagagaatc catcgacgcc atacaagcct aagtcacgca
      241 ccatcccgcc aaaaatcctg gccggacagg aacaggccag agtatcaagt aatactctgg
      301 ctttcttatg ctcatcacta agcgtaaaaa aatcggcgat acgctgttca agcagttggg
      361 tgtttgagac cacattcata cagtgatttt agctcatcgt gaaggcgcat taaataacta
      421 tccgtcatgc ctgagattgc gtcg
//
LOCUS       DAEEGA010000322          418 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.336, whole genome shotgun sequence.
ACCESSION   DAEEGA010000322 DAEEGA010000000
VERSION     DAEEGA010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.336"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..373
                     /gene="istB"
                     /locus_tag="KLH46_004457"
     CDS             <1..373
                     /gene="istB"
                     /locus_tag="KLH46_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HBE0380817.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT          121 a          100 c          105 g           92 t
ORIGIN      
        1 aacagcagca gatctgttac ttcagttatc tacggcacaa cgtcagggcc gttataaaac
       61 gacgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa taggctatct
      121 gccgttcagt caggaagaag caaaactgtt cttccaggtc atcgctaaac gttacgaaaa
      181 gagcgcaatg atcctgacat ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg
      241 tgatgcagca ctgacctcag cgatgctggg ccgtatctta caccactcac atgtcgttca
      301 aatcaaagga gaaagctatc gactcagaca gaaacgaaag gccggggtta tagcagaagc
      361 taatcctgag taaaacggtg gatcaatatt gggccgttgg tggagatata agtggatc
//
LOCUS       DAEEGA010000323          415 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.337, whole genome shotgun sequence.
ACCESSION   DAEEGA010000323 DAEEGA010000000
VERSION     DAEEGA010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.337"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..126
                     /locus_tag="KLH46_004458"
     CDS             <1..126
                     /locus_tag="KLH46_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBE0380818.1"
                     /translation="VSPSQWAAIGVLGSLLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            126..>415
                     /locus_tag="KLH46_004459"
     CDS             126..>415
                     /locus_tag="KLH46_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HBE0380819.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLVTVARQINPYIKVDIPET"
BASE COUNT          117 a           86 c          108 g          104 t
ORIGIN      
        1 gtcagtccat cacagtgggc tgcgattggt gtgctgggaa gtctgcttct ggggcttctg
       61 acttatctga cgaatctgta tttcaaaata agagaagata agcgtaaggc tgcgagaggt
      121 gaataatgtc gccatcatta cgcaaggctg ttgcagttgc tattggtggc ggggctgttg
      181 ctatagcatc tgtgttaatc actggcccag gtggtaacga tggtctggaa ggtgtcagct
      241 acataccata caaagatatc attggtgtat ggactgtatg tcacggacac accggaaaag
      301 acatcatgcc tggtaaaacg tataccgaag cagaatgcaa agctctcctg aataaagacc
      361 ttgtcactgt cgccagacaa attaacccgt acatcaaagt cgatataccg gaaac
//
LOCUS       DAEEGA010000324          407 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.338, whole genome shotgun sequence.
ACCESSION   DAEEGA010000324 DAEEGA010000000
VERSION     DAEEGA010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.338"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..>407
                     /locus_tag="KLH46_004460"
     CDS             <1..>407
                     /locus_tag="KLH46_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0380820.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLI"
BASE COUNT           85 a          144 c          102 g           76 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatc
//
LOCUS       DAEEGA010000325          380 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.341, whole genome shotgun sequence.
ACCESSION   DAEEGA010000325 DAEEGA010000000
VERSION     DAEEGA010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.341"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            1..380
                     /locus_tag="KLH46_004461"
                     /pseudo
     CDS             1..380
                     /locus_tag="KLH46_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           94 a          104 c          109 g           73 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc
//
LOCUS       DAEEGA010000326          376 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.342, whole genome shotgun sequence.
ACCESSION   DAEEGA010000326 DAEEGA010000000
VERSION     DAEEGA010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.342"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(229..>376)
                     /locus_tag="KLH46_004462"
     CDS             complement(229..>376)
                     /locus_tag="KLH46_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0380821.1"
                     /translation="RYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNI
                     KHYQ"
BASE COUNT          121 a           71 c           58 g          126 t
ORIGIN      
        1 tggagtcatt accttaaatc aagcatacta aatccaataa gtgacaaatc tttaatttat
       61 agatttcatg aatatttaaa tattaactga acgagtaatt cattgattca acatctaatg
      121 actatccaac aaataaatat gcgtgatata taaagccctt tctttagttt attttcagaa
      181 aaagatacac aaaatttata tttggcattt tctggtaatg actccaactt actgatagtg
      241 ttttatgttc agataatgcc cgatgacctt gtcatgcagc tccaccgatt ttgagaacga
      301 cagtgacttc cgtcccagcc ttgccagatg ttgtctcaga ttcagattat gtcgctcaat
      361 gcgctgagtg taacgc
//
LOCUS       DAEEGA010000327          375 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.343, whole genome shotgun sequence.
ACCESSION   DAEEGA010000327 DAEEGA010000000
VERSION     DAEEGA010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.343"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..>375
                     /locus_tag="KLH46_004463"
     CDS             <1..>375
                     /locus_tag="KLH46_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0380822.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAEEGA010000328          372 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.denovo.344, whole genome shotgun sequence.
ACCESSION   DAEEGA010000328 DAEEGA010000000
VERSION     DAEEGA010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.denovo.344"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..311)
                     /locus_tag="KLH46_004464"
     CDS             complement(<1..311)
                     /locus_tag="KLH46_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0380823.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAEEGA010000329         7481 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.guided.146, whole genome shotgun sequence.
ACCESSION   DAEEGA010000329 DAEEGA010000000
VERSION     DAEEGA010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.guided.146"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..1293
                     /locus_tag="KLH46_004465"
     CDS             <1..1293
                     /locus_tag="KLH46_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBE0380824.1"
                     /translation="AINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGM
                     ALSHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETP
                     LGEPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFI
                     CELAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGAN
                     RLGSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGEN
                     WAKIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYT
                     IELSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRL
                     EYSDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            1286..2020
                     /gene="frdB"
                     /locus_tag="KLH46_004466"
     CDS             1286..2020
                     /gene="frdB"
                     /locus_tag="KLH46_004466"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBE0380825.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2031..2426
                     /gene="frdC"
                     /locus_tag="KLH46_004467"
     CDS             2031..2426
                     /gene="frdC"
                     /locus_tag="KLH46_004467"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBE0380826.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            2437..2796
                     /gene="frdD"
                     /locus_tag="KLH46_004468"
     CDS             2437..2796
                     /gene="frdD"
                     /locus_tag="KLH46_004468"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBE0380827.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            2859..3992
                     /locus_tag="KLH46_004469"
     CDS             2859..3992
                     /locus_tag="KLH46_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBE0380828.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            4081..4614
                     /gene="blc"
                     /locus_tag="KLH46_004470"
     CDS             4081..4614
                     /gene="blc"
                     /locus_tag="KLH46_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBE0380829.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4611..4928)
                     /gene="sugE"
                     /locus_tag="KLH46_004471"
     CDS             complement(4611..4928)
                     /gene="sugE"
                     /locus_tag="KLH46_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBE0380830.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(5104..5250)
                     /gene="ecnB"
                     /locus_tag="KLH46_004472"
     CDS             complement(5104..5250)
                     /gene="ecnB"
                     /locus_tag="KLH46_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBE0380831.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(5361..5486)
                     /gene="ecnA"
                     /locus_tag="KLH46_004473"
     CDS             complement(5361..5486)
                     /gene="ecnA"
                     /locus_tag="KLH46_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBE0380832.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(5538..6104)
                     /gene="efp"
                     /locus_tag="KLH46_004474"
     CDS             complement(5538..6104)
                     /gene="efp"
                     /locus_tag="KLH46_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBE0380833.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            6146..7174
                     /gene="epmB"
                     /locus_tag="KLH46_004475"
     CDS             6146..7174
                     /gene="epmB"
                     /locus_tag="KLH46_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBE0380834.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1867 a         1921 c         1946 g         1747 t
ORIGIN      
        1 gcaataaaca tgatggaagg cacgttggtg cagatccgtg ctaacgcggt cgttatggct
       61 accggcggtg cgggtcgcgt ttatcgttac aacaccaacg gcggcatcgt taccggtgac
      121 ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat
      181 cacccaaccg gtctgccagg ttccggtatc ctgatgaccg aaggctgccg tggtgaaggc
      241 ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag attacggcat gggcccggaa
      301 actccgctgg gcgagccgaa aaacaaatat atggaactgg gtccacgcga caaagtttct
      361 caggccttct ggcacgaatg gcgtaaaggc aacaccatcc ccacgccgcg tggtgatgtg
      421 gtttatctcg acctgcgtca cctcggcgag aaaaaactgc atgaacgtct gccgttcatc
      481 tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta aagaaccgat tccggtacgt
      541 ccgactgcac actacaccat gggcggtatc gaaaccgatc agaactgtga aacccgcatt
      601 aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg
      661 ggctccaact ccctggcgga actggtggtc ttcggccgtc tggccggtga acaagcgaca
      721 gagcgtgcag caactgccgg taatggcaac gaagcggcaa ttgaagcgca ggcagctggc
      781 gttgaacaac gtctgaaaga tctagttaac caggatggcg gcgaaaactg ggcgaagatc
      841 cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta tctaccgtac gccggaactg
      901 atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac gcttcaagcg cgtgcgcatc
      961 accgacacct ccagcgtgtt caacaccgac ctgctctaca ccattgaact gagccacggt
     1021 ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca
     1081 caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg tcaactttct caaacacacc
     1141 ctcgccttcc gcgatgctga tggcacgact cgcctggagt acagcgacgt gaagattact
     1201 acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg atgcagccga taaggcggaa
     1261 gcagccaata ataaggagaa ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt
     1321 gcgctataac ccggaagtcg ataccgcacc gcatagcgca ttctatgaag tgccttatga
     1381 cgcaactacc tcattactgg atgcgctggg ctacatcaaa gacaacctgg caccggacct
     1441 gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga tggttaacaa
     1501 cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac accgacggta tgaaggttga
     1561 agcgttagct aacttcccga ttgaacgcga tctggtggtc gatatgaccc acttcatcga
     1621 aagtctggaa gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg atcagggtac
     1681 taacatccag accccggcgc agatggcaaa gtatcaccag ttctccggtt gcatcaactg
     1741 tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac ccagagttca tcggtccggc
     1801 tgccattacg ctggcgcatc gttataacga agatagccgc gaccacggta agaaggagcg
     1861 tatggcgcag ttgaacagcc agaacggtgt atggagctgt actttcgtgg gctattgctc
     1921 cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt cagcagggca aagtagaaag
     1981 ttcgaaagac tttcttatcg cgaccctgaa accacgctaa ggagtgcaac atgacgacta
     2041 aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc
     2101 gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc attgaactga
     2161 ttttcgggct gtttgccctg aaaaatggcc cggaagcctg ggcgggattc gtcgactttt
     2221 tacaaaaccc ggttatcgtg atcattaacc tgatcactct gacggcagct ctgctgcaca
     2281 ccaaaacctg gtttgaactg gcaccgaaag cggccaatat cattgtaaaa gacgaaaaaa
     2341 tgggaccaga gccaattatc aaaagtctct gggcggtaac tgtggttgcc accatcgtaa
     2401 tcctgtttgt tgccctgtac tggtaaggag cctgagatga ttaatccaaa tccaaagcgt
     2461 tctgacgaac cggtattctg gggcctcttc ggggccggtg gtatgtggag cgcaattatt
     2521 gcgccggtga tgatcctgct ggtgggtatt ctgctgccac tggggctgtt tccgggtgat
     2581 gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg cgtattcctg
     2641 ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac
     2701 gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct acggtctggc tgctatcctg
     2761 acagttgtca cgctgattgg tgtcgttaca atctaacgca tcgccaatgt aaatccggcc
     2821 cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg acgctctgcg
     2881 ccttattaat taccgcctct tactccacat ttgctgcccc tcaacaaatc aacgatattg
     2941 tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg gcggtggcgg
     3001 taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc
     3061 agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg
     3121 tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat
     3181 actggcctga acttaccgct aaacagtgga atgggatcac actattacat cttgcgacct
     3241 acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt gaaatcctca agcgacttgc
     3301 tgcgcttcta tcaaaactgg cagcctgcat gggctccagg aacacaacgt ctgtatgcca
     3361 actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc
     3421 aggcgatgca aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg attaatgtac
     3481 ctcccccaga agaaaagaat tacgcctggg gatatcgcga aggtaaggca gtgcatgttt
     3541 cgccaggggc gttagatgct gaagcttatg gtgtgaagtc gaccattgaa gatatggccc
     3601 gctgggtgca aagcaattta cccccccttg atatcaatga gaaaacgctt caacaaggga
     3661 tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc ctgggctggg
     3721 aaatgctgga ctggccggtc aatcctgaca gcatcattaa cggcagtgac aataaaattg
     3781 cactggcagc acgcccggta aaagcgatta agcccccaac tcctgcagta cgcgcatcat
     3841 gggtacataa aacgggggcg accggcggat ttggtagcta tgtcgcgttt attccagaaa
     3901 aagagctggg tatcgtgatg ctggctaaca aaaactatcc caatccagcg agagtcgccg
     3961 ccgcctgtca gattctcaac gctctacagt aaaatttcat cgggtccgaa ttttcggacc
     4021 ttttctgcgc ttttccttgc tgtcatctac acttagaaaa aaaccagtaa ggaaacattt
     4081 atgcgcctgc tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgtagt
     4141 tttcctacgc cgccgcgtgg cgtgaccgta gtgaataatt tcgacgccaa acgttatctc
     4201 ggtacctggt atgagattgc ccgttttgat caccgttttg aacgtggact ggaaaaagtc
     4261 accgcaacat acagcctgcg tgatgacggc ggcctgaatg tcattaataa aggctataac
     4321 cctgacagag gaatgtggca gcagagtgaa gggaaagcgt actttaccgg cgcaccaact
     4381 cgcgctgcgc tgaaagtgtc attctttggt cctttctatg gcggttataa cgttattgca
     4441 ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg accgcgacta cctgtggata
     4501 ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc
     4561 cgggaagggt ttgatgtcag taagtttatt tgggtacagc agcccggtag ttagtgagtg
     4621 ctgagtttca gaccaataat ccccaatacg attaacgcca gactcgccag acgcatcggg
     4681 ttagcggact caccgagcag cacaatgccg gtgatggccg cgccgactgc gccaataccc
     4741 gtccacacgg cataagccgt ccctaccggt aacgatttca tcgcccaggc aagtagcgcc
     4801 atactgacta tcatcgccgt cacggtaata acactcggcg tcaaacggct aaagccgtgg
     4861 gtatatttca ggccaacggc ccataccact tccagcagac cagcaataac taagataatc
     4921 caggacatat caggctccgg aaatttgggg ccgtccccgg tgaaaagagg cgtttgcagg
     4981 tcgtcctgca aagctgatgg ttgaacggat attttgccag gtggcagggg aatttcaact
     5041 attaccttta tgacgatcgc agaaaaaaca aacggcacga cactgctgtc gtaccgttat
     5101 tgcttattgc tgcgctttcg ttgctgcgcc agaaatcgcg ttaccgccat cagaaatgtc
     5161 ttcaccaacg ccacgcgtgg tgttgcaggc agttaatact gttgaaagca ccagaacaga
     5221 aaagatcgct gcaattgtct tcttcaccat aacgtcttcc ttttaggtaa tgttatttat
     5281 gtttgcctat agcaaattaa taatagacaa catcgccaaa aatgatggaa atctgatgat
     5341 ttttcggaag agaagaaaaa ttagctggca gcgcgagaga tggagttacc gagatgtttg
     5401 atgtcttcgc cgaaaccgca agcggtgtta cagcccgtga gcagcgtgct ggcaagcaaa
     5461 acaagaacga taaggcgttt catcatctct gacctgaata agtgatggtg cagcctgcag
     5521 gccgcaccac aaccgcatta cttcacgcga gagacgtatt caccagagcg ggtatccact
     5581 ttgatgactt cgccgatttg tacgaacagc ggaactttaa ccacagcgcc agtagacagg
     5641 gtagccggtt tgccgccagt acctgcggta tcacctttca ggcccggatc ggtatcaacg
     5701 atttccagtt caacgaagtt cggcggagta acggagatcg gctgaccatt ccacagagtt
     5761 acgatacact ctgcctgatc cagcagccat ttagcattgt cgccaattgc ttttgcatca
     5821 gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc agaactcacc gtcgttgtac
     5881 aggtaagtca ggttcatatc gacaacatca gcgccttcag cggaatcagt agatttgaag
     5941 gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt taacgcgagc aaatgcctgg
     6001 cctttacccg gttttacgaa ttcactcgct tcaaccgcgt aaggttcgcc gtctaacatg
     6061 attttaagac cagcacgaaa atcgttgcta tagtacgttg ccataaggcc ctctgaaatt
     6121 tgttaattgg tagctaagcc acaaaatggc gcatattgta accctaaata ccccatccag
     6181 agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat cctgatgaac ttctgcgtct
     6241 tttgaatata gacgcggacg aaaaactgtt ggccggacgc agcgccaaaa agctgtttgc
     6301 cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa ggcaatccgg acgatcctct
     6361 tttgcgtcag gtacttacct cgcaagatga gtttgtcgtc gcgtccggat tctccaccga
     6421 cccactggaa gaacagcaca gcgtagtgcc tggtttgttg cataaatacc acaaccgggc
     6481 gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc tattgcttcc gtcgccactt
     6541 cccctatgcc gaaaatcagg gcaacaagcg taactggcag actgcacttg agtatgttgc
     6601 tgcgcatccg gaactggacg agatgatttt ctccggcggc gatccgctga tggcgaaaga
     6661 gcacgagctg gactggttgc tcacacaact ggaagccatc ccgcatatca aacgtctgcg
     6721 gattcacagc cgtctgccga ttgtgatccc ggcacgtatc accgaggcgc tggttgaacg
     6781 ctttgcccgt tctacgctgc aaatcttgct ggtgaatcac atcaatcatg ccaatgaggt
     6841 agatgaaacg ttccgtcagg cgatggctaa attgcgccgt gtcggtgtca ccctgcttaa
     6901 ccagagcgtt ctgttacgtg gtgtgaacga taacgcacaa acgttggcaa acctgagtaa
     6961 tgcgttgttc gatgccggcg tgatgccgta ttacctgcat gtgctagata aagtacaggg
     7021 cgcggcgcat tttatggtga gtgatgacga agcacggcag attatgcgtg agttgctgac
     7081 attggtgtcg ggatatctgg tgccgaaact ggcgcgagaa attggcggcg aacccagcaa
     7141 aacgccgctg gatctccagc tacgccagca gtaactgatt aaacagggaa gttaaacatt
     7201 aaaattgcct gatgcgctac gcttatcagg cctacatcgt gcattgaaat tgaatggatt
     7261 tgcaatgaac tgtaggccgg ataaggcgtt tacgccgcat ccggcaagag caacaaacgc
     7321 aagtacagca acctgtcatt aaactgagaa atttaatctt tgcacgttgc ataaccttta
     7381 attattcata tgcattgctg ttgttacttt ttatatagct aacaccaata attaaccttt
     7441 cttttcggta atgactccaa cttactgata gtgttttatg t
//
LOCUS       DAEEGA010000330         4556 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.guided.198, whole genome shotgun sequence.
ACCESSION   DAEEGA010000330 DAEEGA010000000
VERSION     DAEEGA010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.guided.198"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..238)
                     /locus_tag="KLH46_004476"
     CDS             complement(<1..238)
                     /locus_tag="KLH46_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HBE0380835.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLV"
     gene            complement(241..801)
                     /locus_tag="KLH46_004477"
     CDS             complement(241..801)
                     /locus_tag="KLH46_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE0380836.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(927..1277)
                     /locus_tag="KLH46_004478"
     CDS             complement(927..1277)
                     /locus_tag="KLH46_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBE0380837.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(1480..2493)
                     /gene="intI1"
                     /locus_tag="KLH46_004479"
     CDS             complement(1480..2493)
                     /gene="intI1"
                     /locus_tag="KLH46_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBE0380838.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2703..3533
                     /locus_tag="KLH46_004480"
     CDS             2703..3533
                     /locus_tag="KLH46_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HBE0380839.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            3646..4437
                     /gene="aadA1"
                     /locus_tag="KLH46_004481"
     CDS             3646..4437
                     /gene="aadA1"
                     /locus_tag="KLH46_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBE0380840.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1150 a         1209 c         1205 g          992 t
ORIGIN      
        1 cgaccagctt caacaagggc gggaacggta gttcatcgac gcccaggatg acgccgggaa
       61 agcgcaggta acagagctgc accgcgaagc ccagccgatt ggctggcccg cgccgctgtc
      121 ggatgatcga gaggtcggta tcgttgaatg tgtaatgtcg gatcaggtcg tccttggagt
      181 ccggcaacgc cagcaggctt tcccgctcgg cggcggacag gatggaacga cgtggcatat
      241 ttactgatcc gttctcaagt attgatacag ggtttcgcga ctgattccga attcacgagc
      301 aagcttggtc ttttgctcgc cagcctcgac acgttggcgc agttcggcaa tacgctcaga
      361 cgacagggat ttcttcctgc cacggtaagc cccgcgttgc ttggcgagcg caataccctc
      421 gcgctgacgc tcgcggatca gggcgcgctc gaactcggcg aacgcgccca tcaccgagag
      481 catcaggttc gccatcggag agtcttcgcc agtaaaactg aggtgttcct tgacgaattc
      541 gatatgcacg ccgcgttgtg tcagcgtttg cacgatccgg cgcaaatcat cgagattgcg
      601 cgccaggcga tccatgctat gcaccaccac ggtgtcgccg gtgcgggcga agcttatcag
      661 cgcttccagt tgcggacgct tgacatcctt gccggatgcc ttgtcgctaa aagcgcgatc
      721 aaccttgacg ccttccagtt gccgttccgg gttctggtcg aaggtgctga ccctgatata
      781 cccaatgcgc tgtccagtca tggaattccc tgcaaaatgt cagggaagac tctatgacct
      841 tcaacgagat atgtcaataa attcaaaatt caatcctatc ctgacgcaat ttacacatgg
      901 catctgacat caggttaggg tatgcctcaa cctgacggcg gcgaatcaca agcgtccggt
      961 ttgacgctgg tttcggtcgc agtgctggcc cgcgcctgga agcgctgata gcactccagc
     1021 ccgcagaaat gctccacgta ctcggcccct tccggcgtga aggcggcatc gagcgggatt
     1081 tccttgcagc acacgcagca actggtgctc ggttcattgg cgttcatggt ggtgttcctc
     1141 cattggttga cgaagccgac gaaggccgcc gccggcatcg gcctggcgaa caggaaaccc
     1201 tgcaccgtgt cgcaacccgc ctgccgcaac cacgcaaggc agtcgggtgt ttccacaccc
     1261 tcggctacca cctccattgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     1321 tgcactatag cagcggaggg gttggatcca tcaggcaacg acgggctgct gccggccatc
     1381 agcggacgca gggaggactt tccgcaaccg gccgttcgat gcggcaccga tggccttcgc
     1441 gcaggggtag tgaatccgcc aggattgact tgcgctgccc tacctctcac tagtgagggg
     1501 cggcagcgca tcaagcggtg agcgcactcc ggcaccgcca actttcagca catgcgtgta
     1561 aatcatcgtc gtagagacgt cggaatggcc gagcagatcc tgcacggttc gaatgtcgta
     1621 accgctgcgg agcaaggccg tcgcgaacga gtggcggagg gtgtgcggtg tggcgggctt
     1681 cgtgatgcct gcttgttcta cggcacgttt gaaggcgcgc tgaaaggtct ggtcatacat
     1741 gtgatggcga cgcacgacac cgctccgtgg atcggtcgaa tgcgtgtgct gcgcaaaaac
     1801 ccagaaccac ggccaggaat gcccggcgcg cggatacttc cgctcaaggg cgtcgggaag
     1861 cgcaacgccg ctgcggccct cggcctggtc cttcagccac catgcccgtg cacgcgacag
     1921 ctgctcgcgc aggctgggtg ccaagctctc gggtaacatc aaggcccgat ccttggagcc
     1981 cttgccctcc cgcacgatga tcgtgccgtg atcgaaatcc agatccttga cccgcagttg
     2041 caaaccctca ctgatccgca tgcccgttcc atacagaagc tgggcgaaca aacgatgctc
     2101 gccttccaga aaaccgagga tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg
     2161 ccgcgacggc cgaggtcttc cgatctcctg aagccagggc agatccgtgc acagcacctt
     2221 gccgtagaag aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg
     2281 ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg
     2341 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
     2401 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     2461 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     2521 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     2581 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttgggcga
     2641 acccggagcc tcattaattg ttagccgtta aaattaagcc ctttaccaaa ccaatactta
     2701 ttatgaaaaa cacaatacat atcaacttcg ctattttttt aataattgca aatattatct
     2761 acagcagcgc cagtgcatca acagatatct ctactgttgc atctccatta tttgaaggaa
     2821 ctgaaggttg ttttttactt tacgatgcat ccacaaacgc tgaaattgct caattcaata
     2881 aagcaaagtg tgcaacgcaa atggcaccag attcaacttt caagatcgca ttatcactta
     2941 tggcatttga tgcggaaata atagatcaga aaaccatatt caaatgggat aaaaccccca
     3001 aaggaatgga gatctggaac agcaatcata caccaaagac gtggatgcaa ttttctgttg
     3061 tttgggtttc gcaagaaata acccaaaaaa ttggattaaa taaaatcaag aattatctca
     3121 aagattttga ttatggaaat caagacttct ctggagataa agaaagaaac aacggattaa
     3181 cagaagcatg gctcgaaagt agcttaaaaa tttcaccaga agaacaaatt caattcctgc
     3241 gtaaaattat taatcacaat ctcccagtta aaaactcagc catagaaaac accatagaga
     3301 acatgtatct acaagatctg gataatagta caaaactgta tgggaaaact ggtgcaggat
     3361 tcacagcaaa tagaacctta caaaacggat ggtttgaagg gtttattata agcaaatcag
     3421 gacataaata tgtttttgtg tccgcactta caggaaactt ggggtcgaat ttaacatcaa
     3481 gcataaaagc caagaaaaat gcgatcacca ttctaaacac actaaattta taaaaaatct
     3541 aatggcaaaa tcgcccaacc cttcaatcaa gtcgggacgg ccaaaagcaa gcttttggct
     3601 cccctcgctg gcgctcggcg ccccttattt caaacgttaa acatcatgag ggaagtggtg
     3661 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     3721 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     3781 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     3841 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     3901 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     3961 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     4021 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     4081 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     4141 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     4201 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     4261 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     4321 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     4381 ttggaagaat ttgttcacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt
     4441 ctaacaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc
     4501 actaagcaca taattgcggt gatgctgcca acttactgat ttagtgtatg atggtg
//
LOCUS       DAEEGA010000331         4083 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.guided.206, whole genome shotgun sequence.
ACCESSION   DAEEGA010000331 DAEEGA010000000
VERSION     DAEEGA010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.guided.206"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            complement(<1..932)
                     /gene="pepD"
                     /locus_tag="KLH46_004482"
     CDS             complement(<1..932)
                     /gene="pepD"
                     /locus_tag="KLH46_004482"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HBE0380841.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAA"
     gene            1194..1652
                     /gene="gpt"
                     /locus_tag="KLH46_004483"
     CDS             1194..1652
                     /gene="gpt"
                     /locus_tag="KLH46_004483"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBE0380842.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1744..2988
                     /gene="frsA"
                     /locus_tag="KLH46_004484"
     CDS             1744..2988
                     /gene="frsA"
                     /locus_tag="KLH46_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBE0380843.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            3046..3447
                     /gene="crl"
                     /locus_tag="KLH46_004485"
     CDS             3046..3447
                     /gene="crl"
                     /locus_tag="KLH46_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBE0380844.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(3486..>4083)
                     /locus_tag="KLH46_004486"
     CDS             complement(3486..>4083)
                     /locus_tag="KLH46_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBE0380845.1"
                     /translation="YRNTDFFGVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDF
                     GGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMT
                     PITGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYY
                     FNKNMSAFVDYKINQLDSDNKLNINNDDTVAVGMTYQF"
BASE COUNT          988 a         1006 c         1037 g         1052 t
ORIGIN      
        1 gcagctttat cgttcgctac agagtccagc aacaaggcca gatttttctc tttctctgcc
       61 agctcgtttt tcaggatctc ctgataggta ttcaccagag atttcagtgc gtcgacttta
      121 tcagctgcga cagcaatggt cgcaaaggct tcacgcggga tggcgttacg cagtgtgccg
      181 ccgttgaaat cgataaggcg caggtccagt tcttctgcat gacccgccag gaagcgcacc
      241 agcagtttgt tggcattacc caggccaacg tggatttcac cgccggagtg accgcctttc
      301 agacctttta aggttaactt gaaggtttca aaaccagctg gaaccgcttc acgatctaaa
      361 tgcaggttgg aggtgaagtc gatacccccc gcacaaccca tgtagatttc accttcttct
      421 tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt tgctctgtaa gccgaacgca
      481 ccgtccatac cggcttcttc ggtcatggtc agcagcactt ccagcgggcc gtgaaccacg
      541 ttttcgtcag ccagaaccgc cagtgcagag gccataccaa tgccgttatc cgcacccagc
      601 gtggtgccgc gcgctttaac ccattcgcca tcaatataag gctggatagg atctttcgtg
      661 aagtcatgca cggtgtcgtt atttttctgc ggcaccatat cgaggtgggc ctgtaagacg
      721 accggtttac gattttccat acctgcggta gcaggtttac gaatcaggat attacctacc
      781 tgatcgcgtt cgacatggaa acctttctct tttgcccaac caacaatgta ttcagcgagt
      841 tgctcttcat gataggacgg gtgaggaata gaacagattt tggcaaaaat atcccacagc
      901 ggctgtggag ataattgaga cagttcagac acgttaagtc tccttgtcga tcacccgcaa
      961 aacagtattg caggtcacag ggttagcaga aaatgttgtc aacacaagac aggcttgcga
     1021 gatatgtatt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
     1081 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
     1141 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
     1201 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
     1261 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctggtaccgg
     1321 gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct
     1381 acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag
     1441 gcttcatcgt tattgatgac ctggtggata ccggtggtac tgctgttgcg attcgtgaaa
     1501 tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg
     1561 atgactatgt tgttgatatc ccgcaagata cctggatcga acagccgtgg gatatgggcg
     1621 tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca ttgccgggcg
     1681 ttgttctttt taacttcagg caggttacaa tagtttccag taagtattct ggaggctgca
     1741 tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa
     1801 acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggctctt
     1861 gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc
     1921 ggtattgacc cacgtgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgctgaa
     1981 cgtaccgacg atgatttata cgataccgtg attggctacc gtggtggcaa ctggatttat
     2041 gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct gtacggaaga agatccgaaa
     2101 ctcagtggtc gacactggct gcatgcggct acgttgtaca acattgccgc ctatcctcat
     2161 ctgaaaggag atgacctggc cgagcaagcg caggctttat caaaccgcgc ctatgaagag
     2221 gccgcccagc gtctgccggg cacgatgcgg cagatggagt ttaccgtacc cggcggtgcg
     2281 cccatcaccg gctttttgca tatgccgaaa ggcgatggcc cgttcccgac agtattaatg
     2341 tgtggtggtc tggatgcgat gcagacggac tattacagcc tgtatgaacg ttattttgcg
     2401 ccgcgcggca ttgcgatgct gactattgat atgccgtcgg tgggcttttc ttcaaaatgg
     2461 aagctcaccc aggactccag cctgttgcat cagcacgtct taaaggcgct gcctaacgta
     2521 ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc gtttcggcgc taacgttgcc
     2581 gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg ttgcctgtct tggtccggta
     2641 gttcataccc tgttgagtga ttttaagtgc cagcaacagg tgccggaaat gtatcttgac
     2701 gttctggcga gtcgtttggg gatgcatgat gcttccgacg aagcgttacg cgtggagctg
     2761 aatcgctatt cattaaaagt gcaaggattg ctggggcgtc gctgcccaac gccaatgtta
     2821 tcaggctact ggaagaacga tccgttcagc ccggaagagg actcacgctt aatcacctca
     2881 tcatctgctg acggtaaatt attagagatc ccatttaacc cggtgtatcg gaattttgac
     2941 aaaggtcttc aggaaatcac cggctggatc gaaaaacgct tgtgttaaaa atttgctaaa
     3001 ttttgccaat ttggtaaaac agttgcatca caacaggaga tagcaatgac gttaccgagt
     3061 ggacacccga agagcagatt gatcaaaaaa tttaccgcac taggcccgta tattcgtgaa
     3121 ggtaagtgcg aagataatcg attctttttc gattgtctgg ctgtatgcgt caacgtgaaa
     3181 ccggcaccgg aagtgcgtga attctggggc tggtggatgg agctggaagc gcaggaatcc
     3241 cgttttacct acagctacca gtttggtctg ttcgataaag caggcgactg gaagagtgtt
     3301 ccggtaaaag atactgaagt ggttgaacga ctggagcaca ccctgcgtga gtttcacgag
     3361 aagctgcgtg aactgctgac gacgctgaat ctgaagctgg aaccggcgaa tgattttcgt
     3421 gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg gatgcatcac atccggcaat
     3481 attcattaaa actgatacgt catcccaacc gcgacagtat catcattatt aatattcagc
     3541 ttattatcgc tatccagttg gttgatttta taatcaacaa acgctgacat atttttgttg
     3601 aaataatacg tagcaccgac gtcgatataa ttgaccagat cttcatcacc gatgccctca
     3661 atatctttcc ctttcgataa gacataaccc agcgatggac gcagaccaaa gtcaaactgg
     3721 tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa agccgccagt tattggcgtc
     3781 attttgcgtg tttcagaata gaaagttgcc agataaatat tattggcatc gtatttcaga
     3841 cctgttgccc atgcttctgc acgcttgcct gtgccacggc tttgcaggtt ctgctcgttg
     3901 gtgcgatctg agttggtata ggccccacta atggcgaaat cgctgccgcc aaagtcatat
     3961 gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa cgtcgcggtt ttcgtttttc
     4021 ccttgatatt gcagggttaa gttcaggcca tcgataacgc cgaagaagtc ggtgttccga
     4081 tac
//
LOCUS       DAEEGA010000332         3310 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.guided.217, whole genome shotgun sequence.
ACCESSION   DAEEGA010000332 DAEEGA010000000
VERSION     DAEEGA010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.guided.217"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            <1..206
                     /locus_tag="KLH46_004487"
     CDS             <1..206
                     /locus_tag="KLH46_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBE0380846.1"
                     /translation="DVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTR
                     HLGYREVTITEKGYAAFKTHFHI"
     gene            complement(184..807)
                     /gene="tetR(B)"
                     /locus_tag="KLH46_004488"
     CDS             complement(184..807)
                     /gene="tetR(B)"
                     /locus_tag="KLH46_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HBE0380847.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            889..2094
                     /gene="tet(B)"
                     /locus_tag="KLH46_004489"
     CDS             889..2094
                     /gene="tet(B)"
                     /locus_tag="KLH46_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBE0380848.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2207..2800)
                     /gene="tetC"
                     /locus_tag="KLH46_004490"
     CDS             complement(2207..2800)
                     /gene="tetC"
                     /locus_tag="KLH46_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HBE0380849.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2933..3304
                     /locus_tag="KLH46_004491"
     CDS             2933..3304
                     /locus_tag="KLH46_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0380850.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          894 a          606 c          680 g         1130 t
ORIGIN      
        1 ttgacgttcc ttccaaacat tcacgtaaaa tctgttgtgc gtgtttagat tggagtgaac
       61 gccgtttcca tttaggtggg tacgttggag ccgcattatt ttcgctttat gaatctaaag
      121 ggtggttaac tcgacatctt ggttaccgtg aagttaccat cacggaaaaa ggttatgctg
      181 cttttaagac ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag
      241 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa
      301 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc
      361 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc
      421 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata
      481 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa
      541 agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa
      601 agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc
      661 ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag
      721 tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat
      781 cactttactt ttatctaatc tagacatcat taattcctaa tttttgttga cactctatca
      841 ttgatagagt tattttacca ctccctatca gtgatagaga aaagtgaaat gaatagttcg
      901 acaaagatcg cattggtaat tacgttactc gatgccatgg ggattggcct tatcatgcca
      961 gtcttgccaa cgttattacg tgaatttatt gcttcggaag atatcgctaa ccactttggc
     1021 gtattgcttg cactttatgc gttaatgcag gttatctttg ctccttggct tggaaaaatg
     1081 tctgaccgat ttggtcggcg cccagtgctg ttgttgtcat taataggcgc atcgctggat
     1141 tacttattgc tggctttttc aagtgcgctt tggatgctgt atttaggccg tttgctttca
     1201 gggatcacag gagctactgg ggctgtcgcg gcatcggtca ttgccgatac cacctcagct
     1261 tctcaacgcg tgaagtggtt cggttggtta ggggcaagtt ttgggcttgg tttaatagcg
     1321 gggcctatta ttggtggttt tgcaggagag atttcaccgc atagtccctt ttttatcgct
     1381 gcgttgctaa atattgtcgc tttccttgtg gttatgtttt ggttccgtga aaccaaaaat
     1441 acacgtgata atacagatac cgaagtaggg gttgagacgc aatcgaattc ggtatacatc
     1501 actttattta aaacgatgcc cattttgttg attatttatt tttcagcgca attgataggc
     1561 caaattcccg caacggtgtg ggtgctattt accgaaaatc gttttggatg gaatagcatg
     1621 atggttggct tttcattagc gggtcttggt cttttacact cagtattcca agcctttgtg
     1681 gcaggaagaa tagccactaa atggggcgaa aaaacggcag tactgctcgg atttattgca
     1741 gatagtagtg catttgcctt tttagcgttt atatctgaag gttggttagt tttccctgtt
     1801 ttaattttat tggctggtgg tgggatcgct ttacctgcat tacagggagt gatgtctatc
     1861 caaacaaaga gtcatcagca aggtgcttta cagggattat tggtgagcct taccaatgca
     1921 accggtgtta ttggcccatt actgtttgct gttatttata atcattcact accaatttgg
     1981 gatggctgga tttggattat tggtttagcg ttttactgta ttattatcct gctatcgatg
     2041 accttcatgt taacccctca agctcagggg agtaaacagg agacaagtgc ttagttattt
     2101 cgtcaccaaa tgatgttatt ccgcgaaata taatgaccct cttgataacc caagagggca
     2161 ttttttacga taaagaagat ttagcttcaa ataaaaccta tctattttat ttatctttca
     2221 agctcaataa aaagccgcgg taaatagcaa taaattggcc ttttttatcg gcaagctctt
     2281 ttaggttttt cgcatgtatt gcgatatgca taaaccagcc attgagtaag tttttaagca
     2341 catcatcatc ataagcttta agttggttct cttggatcaa tttgctgaca atggcgttta
     2401 ccttaccagt aatgtattca aggctaattt tttcaagttc attccaacca atgataggca
     2461 tcacttcttg gatagggata aggtttttat tattatcaat aatataatca agataatgtt
     2521 caaatatact ttctaaggca gaccaaccat ttgttaaatc agtttttgtt gtgatgtagg
     2581 catcaatcat aattaattgc tgcttataac aggcactgag taattgtttt ttatttttaa
     2641 agtgatgata aaaggcacct ttggtcacca acgcttttcc cgagatctca tctattgaaa
     2701 cagcttgata gcctttttca acaaacaata ttcgtgctga gttaaccagt gattgatagg
     2761 tactcttaaa attttcttgt tgatgatttt tattttccat gatagattta aaataacata
     2821 ccgtcagtat gtttatggta tcatgatgat gtggtcgtga caatcttaag aacatttagg
     2881 ttattttatg tatattgaac agcattctcg ctatcaaaat aaagctaata acatccaatt
     2941 aagatatgat gataagcagt ttcatacaac ggttatcaaa gatgttctat tatggattga
     3001 acataattta gatcagtctt tactgcttga tgatgtggcg aataaagcgg gttataccaa
     3061 gtggtatttt cagcggctgt tcaaaaaagt aacaggggtc acactggcta gctatattcg
     3121 tgctcgtcgt ttgacgaaag cggctgttga gttgaggttg acgaaaaaaa ctatccttga
     3181 gatcgcatta aaatatcaat ttgattccca acaatctttt acacgtcgat ttaagtacat
     3241 ttttaaggtt acaccaagtt attatcggcg taataaatta tgggaattgg aggcaatgca
     3301 ctgagagatc
//
LOCUS       DAEEGA010000333         2602 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri 1b strain SF105577
            SAMN17129069-rid12250813.guided.232, whole genome shotgun sequence.
ACCESSION   DAEEGA010000333 DAEEGA010000000
VERSION     DAEEGA010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17129069
            Sequence Read Archive: SRR13348478
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 1b
  ORGANISM  Shigella flexneri 1b
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042365.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042365.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 22:21:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,494
            CDSs (total)                      :: 4,399
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 95
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 408
            CDSs (without protein)            :: 408
            Pseudo Genes (ambiguous residues) :: 0 of 408
            Pseudo Genes (frameshifted)       :: 178 of 408
            Pseudo Genes (incomplete)         :: 132 of 408
            Pseudo Genes (internal stop)      :: 148 of 408
            Pseudo Genes (multiple problems)  :: 47 of 408
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Shigella flexneri 1b"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17129069-rid12250813.guided.232"
                     /strain="SF105577"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:41434"
                     /country="Canada: Montreal"
                     /lat_lon="45.4342 N 73.9070 W"
                     /collection_date="2018-10"
                     /collected_by="LSPQ"
     gene            264..923
                     /locus_tag="KLH46_004492"
     CDS             264..923
                     /locus_tag="KLH46_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HBE0380851.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="KLH46_004493"
     CDS             complement(1124..1501)
                     /locus_tag="KLH46_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0380852.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..>2602)
                     /locus_tag="KLH46_004494"
     CDS             complement(1568..>2602)
                     /locus_tag="KLH46_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBE0380853.1"
                     /translation="HTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQNFRTAS
                     KAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLYHESDLR
                     IEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKPVIGGTL
                     NIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFI
                     LDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTA
                     AIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSAKIGAGK
                     FRPLRPLQPA"
BASE COUNT          599 a          655 c          718 g          630 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1741 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1801 cccggtagcg ctgctgctcg aaactgcggt cacggatttc accaaggcgg ttgaagaaca
     1861 cggcacgggc cagcgcattg cgcgcctcgc ccttgttcag cccggcatgc acgcggcggc
     1921 gtagctcgac gctttgcagc cagtcgagga tgaacagcgt gcgctcgatg cggcccaact
     1981 cgcgcagcgc gacggccaag ccgttctggc gcgggtagct gccgagtttc ctgagcatca
     2041 gcgaggccgt caccgtgccc tgcttgatcg aggtggccag ccgcaggatt tcgtcccaat
     2101 gggcgcggac gtgcttgatg ttgagcgtgc cgccgatcac cggcttgagc gcgtcatagg
     2161 cggcatcgcc cttcgggatg tagagcttgg tgtcgcccag gtcgcggatg cgcggcgcga
     2221 agcggaagcc caagaggtgc atcagggcga agacgtgatc ggtgaagccc gccgtgtcgg
     2281 tgtagtgctc ctcgatccgc aggtcggatt cgtggtacag caggccgtcg agcacgtagg
     2341 ttgagtcgcg caggccgaca ttgaccacct tggtgtggaa tggcgcgtat tggtcggaga
     2401 tgtgggtgta gaaagtccgt cctgggctgc tgccatattt tgggttgatg tgccccgtgc
     2461 tctttgcctt gctagcggtt cggaaattct gtccgtccga tgatgatgtg gtgccatcgc
     2521 cccagtgccc ggcaaaggga tgccgaaact gagcgttgac cagttcagcc aacgctgtcg
     2581 agtacgtttc gtcgcgggta tg
//
